LOCUS       DATDRL010000001         5666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3694997, whole genome shotgun sequence.
ACCESSION   DATDRL010000001 DATDRL010000000
VERSION     DATDRL010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3694997"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VI935_00005"
     CDS             <1..1192
                     /locus_tag="VI935_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLE24009.1"
                     /translation="KDMEFEETTSGVNELGEFVHEDDEGFIPLEMADDLMPDIVDSES
                     ELELKGPESEEDEGERQWVPDEQFRLLYVYFKDMASEPLLTAKEEIEVSAKIKKCEAR
                     AREIKALLDGFSKNKNGNSKRFAKTNGHKNGNGRALSKRLERLNGFMRAYSARGRALR
                     DRFVKANLRLVVSVAKRYMGRGLPLPDLIQEGNVGLMRAVERFDHTKGFKFSTYASWW
                     IHQAISRSLLDQTRTIRVPVYVLEQASKVHRISSILHKEMGRKPAPEEIAGKSGISIE
                     GVKRIQDSANDVVHLDSPIIDGEKTTLLEFVPDGGSSAPDSVMAKVALTGRIRGALSL
                     LTPREEEIIRLRFGIDQESTYTLDEIGRKFDLTRERIRQIEKRALEKLATSEVGEVLK
                     SFLE"
     gene            1652..1894
                     /locus_tag="VI935_00010"
     CDS             1652..1894
                     /locus_tag="VI935_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24010.1"
                     /translation="MKDLIPKLLPLLCTGLGEGNTNTDKLTRHILLTDPDRNYNQTKI
                     EIVEALRELKDSGQIQIITIGWELGEEFFYICARRL"
     gene            2100..2441
                     /locus_tag="VI935_00015"
     CDS             2100..2441
                     /locus_tag="VI935_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HLE24011.1"
                     /translation="MERKQLVHKIAEGANITKAQAEKAVTLITQALSTALREGKKVPK
                     YLIPESPTDTPLWSTVFPFGTPLFARRQMFLKEQYIKALRDYREALLEELKQIDKEIE
                     ELEKTEKQTEK"
     gene            2459..2830
                     /locus_tag="VI935_00020"
     CDS             2459..2830
                     /locus_tag="VI935_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24012.1"
                     /translation="MRVLDYIRIVVVSDICTQSSNVTYNIVYSEESAFCNPKRGVFTL
                     SLYDFIDTPNLDIKEIEKIASRLNGQLLIRGKEEDGTITILHLLGLPLSDWLMKNAMM
                     YRLHQVMNSKISTGVLFIYVS"
     gene            complement(2798..3976)
                     /locus_tag="VI935_00025"
     CDS             complement(2798..3976)
                     /locus_tag="VI935_00025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HLE24013.1"
                     /translation="MNPLPQIITSLIHKSGPITFAKFMELALYHPEYGYYTSGKARIG
                     KKGDYYTSPCVHPAFGEIVGRFIGRAYEVVNSPDFTVIEMGAGKGYLALDILDSLKRN
                     NHRLYDKLNYLITEVGTPQREDWKITLKEHMHKIKRFDSLYDLERESNHGVFISNELV
                     DSFPFHRAKFKDGKLLEIFVSLRNEEFIEILDEPSSNALKDYFDGYNLGFEDEQEVEI
                     NLHAKQWLSEVSHILARGFILTIDYGYLAPELYNPSRIRGTFMCYYKHTTNENPYINL
                     GEQDITAHVDFSNLIRVGQSLGLNMVKYTTQGQFLVDWGILDIVERYTGEKEISDISS
                     YRNRIAIKNLFLPETMGDKFKVLIQEKNIKANDKIFYPESNVRISFQKSTDVYKEHPG
                     "
     gene            4622..5179
                     /locus_tag="VI935_00030"
     CDS             4622..5179
                     /locus_tag="VI935_00030"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018033460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HLE24014.1"
                     /translation="MAKKRITKVYTRTGDKGQTSLVGGKRVSKSSARVEAYGDIDELN
                     SVLGIVRVEAQDNEINEITREIQNDLFIIGADLASPSDIQVPRISKESIKGLEKIIDK
                     FLQELEPLKEFILPSGRGGGPYLHLARTVSRRAERRIVRLIEEEQVNENILAYVNRLS
                     DLLFVMARIENKHGNFEETYVRFNK"
     gene            5274..>5666
                     /locus_tag="VI935_00035"
     CDS             5274..>5666
                     /locus_tag="VI935_00035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLE24015.1"
                     /translation="MKKIETQTYILNPKIEEYLRNLTPVQDEVLKKMEGLGNERGFPI
                     IGPLIGRLLYQLAVMTGAKRVFDMGSGFGYSAYWFAKAVRDEGLVVFAELSQKNASLA
                     KEFFRRGGLDNRVEIHVGDALQILEETDG"
BASE COUNT         1800 a          966 c         1211 g         1689 t
ORIGIN      
        1 aaaggacatg gagtttgaag aaacaacttc tggagtaaac gaacttggag agtttgttca
       61 tgaagatgat gagggtttta taccccttga gatggcagat gatctcatgc ctgatatagt
      121 tgactcagag tccgaattag aactaaaagg accggagtcc gaagaagacg aaggggagcg
      181 gcagtgggtc ccagatgagc agtttaggct tctttatgtt tacttcaagg acatggcatc
      241 ggaaccactt cttacagcta aggaggagat agaggtttca gcaaagataa aaaaatgcga
      301 agcaagggca agggagataa aagctcttct ggatggattt tcaaagaata agaatggcaa
      361 tagcaaaagg ttcgcgaaaa ctaatggaca taagaatgga aacggaagag ctttgtcaaa
      421 gcgtttagag agactaaatg ggtttatgag ggcgtactct gcgaggggga gggcgttaag
      481 ggacaggttt gtgaaggcga acttgaggtt agtagttagt gtagcgaaaa gatacatggg
      541 cagagggctt ccacttccag acctcattca ggaaggaaac gttggattaa tgagggcagt
      601 agaaagattc gaccacacaa agggattcaa gttttcaacc tacgcttcgt ggtggataca
      661 tcaagcgata tctaggtcgc ttcttgatca gacaagaaca ataagggttc ctgtgtatgt
      721 gcttgagcag gccagtaagg tacatagaat aagttctata cttcataagg agatgggtag
      781 aaaacctgcg cctgaagaaa ttgcggggaa atctgggatt tctatagagg gtgtaaagcg
      841 tatacaggac tctgcaaatg atgtggtgca tctagattcc ccgataatag atggagagaa
      901 aacaacccta cttgaatttg tcccagatgg tggttcctcc gcaccggatt ctgttatggc
      961 aaaggtagct cttacaggga gaataagagg ggctctttca cttcttacac ctagggaaga
     1021 ggaaataata aggttgagat ttgggattga tcaagagtca acctacacac ttgatgaaat
     1081 tgggaggaag tttgacctta cacgtgagcg tataagacag attgagaaaa gagcgctaga
     1141 aaagcttgca acatctgagg taggagaagt tttaaaaagc ttcctagaat aagaaccttg
     1201 cacctctcaa ctgttatata gcattcgatt ctctggtaat tcttaggtaa tatgccacgg
     1261 cgtagcatac aaggtcgcca ttgtttcatt tattaggtga ttctttctgt agtgacctct
     1321 ccttataaca cctttgaaca ctctacattg taagaatgtt aatcatagat aacccgtatc
     1381 attcacgcaa agggctgggc taaatgtgtt taagttagag aaaaactttt ctaaaactct
     1441 aaagttgtct aagcttcctg tcattttata acaaaggcta tatttattct tcttttcaca
     1501 ttatattccg tcaacttatt acatattgcg atctgttgca cattaaacta tattttgtat
     1561 taatattggc acaggaatta ggattttttg tagcgttatt tatcaaaaaa gtttatagag
     1621 ttttccattt actagataac cggcaaagaa aatgaaagat ttaatcccta aattgcttcc
     1681 gttgttatgc accggtttag gagaagggaa cacaaatacc gataagctaa caagacatat
     1741 tctgcttacc gatccagata ggaactataa ccaaactaaa atagaaattg tagaggcact
     1801 tagggagctc aaagattcag ggcagattca gattataact ataggctggg agctaggaga
     1861 agagtttttc tacatctgcg caaggagatt atagttgtat gtagcctatt ccttgatcag
     1921 taaatatcct gataatagtt gcaggaaaaa ggctttctat atgccttagt gattaatatt
     1981 ttttacttta ggaggaaaaa gtaaagttct atacaaggaa caatatttga ttcattcgga
     2041 agaaattaag ttgaaaaggt tatagtttca ccgaatataa cgcaacggcg ggtaattaaa
     2101 tggaaagaaa gcaacttgta cacaaaattg ctgaaggtgc aaatatcacg aaagctcaag
     2161 ctgaaaaagc tgttactctt atcacacaag ccctttctac cgcgcttaga gaaggcaaga
     2221 aagtccccaa atatctaata cccgaatctc ctactgacac cccgctttgg agcactgtat
     2281 ttccttttgg gacaccatta tttgcgcgga ggcagatgtt tctaaaagaa caatatataa
     2341 aggcgttgag agactacaga gaagctctgt tagaagagct taaacaaatt gacaaagaga
     2401 tcgaggaact ggaaaagaca gaaaagcaaa ctgagaagtg aaactattgg aggattagtt
     2461 gagggtcctc gattacataa gaattgttgt cgtctcagat atttgtactc agtcttcaaa
     2521 tgtgacatac aacattgtgt attctgagga gtcagctttc tgtaatccaa aaaggggagt
     2581 atttacactt agcttatatg attttattga tactcctaat ctagatatta aagagataga
     2641 gaaaatagct tctcgtctaa atggtcagtt gttgataagg ggaaaggagg aagatggaac
     2701 gattacaatt cttcatcttc tgggattgcc gcttagcgac tggttgatga aaaatgctat
     2761 gatgtataga ttacatcagg taatgaattc taaaatctca accggggtgc tctttatata
     2821 cgtcagttga cttttgaaag cttattctaa cattcgattc aggataaaat attttatcat
     2881 tggccttaat atttttctcc tgaataagta ccttgaactt atctcccatc gtctcaggta
     2941 ggaataggtt tttgatagct attctatttc tatatgagga aatatccgaa atttctttct
     3001 cacccgtata tctctcaaca atatccaaga taccccagtc aacaagaaat tggccttgag
     3061 tcgtgtattt aaccatgttt aaccctaaag actgtccaac tcggattaga ttgctgaagt
     3121 ccacatgagc ggtaatatcc tgctctccta gattaatata agggttttcg ttagtagtgt
     3181 gtttgtagta gcacataaac gtgcccctta ttctggaagg gttatataat tcaggcgcaa
     3241 gatagccgta gtcgattgta agaataaaac ctctggctaa aatatgtgac acttcggaaa
     3301 gccattgttt tgcatgaagg tttatctcaa cctcttgttc gtcttcaaac cctaaattat
     3361 aaccatcaaa ataatctttc aaggcattgc tgctgggttc atccagaatc tcaataaact
     3421 cttcgtttcg aagtgaaaca aatatttcta gtagcttgcc gtctttaaac ttggctcggt
     3481 gaaaagggaa ggagtctact agttcgtttg aaataaaaac accatggttg ctttcgcgct
     3541 cgaggtcgta aagagaatca aatcttttta ttttatgcat gtgctctttc aaagtaattt
     3601 tccaatcttc gcgttgcggt gtgccgactt ctgtaatcaa gtagttaagc ttgtcatata
     3661 atctatggtt atttctttta agtgaatcta aaatatcaag cgctagataa cctttccccg
     3721 ctcccatttc tatgacagta aaatcaggtg aattaactac ttcataggca cgaccaataa
     3781 acctgccaac gatctcccca aacgctggat gcacacacgg acttgtataa taatcgccct
     3841 ttttcccaat tctagctttt cccgaagtgt agtaaccata ctcgggatgg taaagcgcaa
     3901 gctccataaa tttagcaaat gtaatcgggc ctgatttatg aattagagat gttattatct
     3961 ggggtagagg gttcattcta tcctaatttc ttttcttata ttttttaaat tccaagcgga
     4021 ttacatgaat aaattttcta gattgttttt gtcccaatcc cattttaatg ggttgcttac
     4081 aatgtattct cgaattttat ttaaggtctt ttcatttctg atgatatgat catagaaaga
     4141 tttatgccat ttgaaacaaa aatcattttg taatgaattg atttcacgcg tcacagatga
     4201 tttgtattgc tgaattattc tagtagggaa cgcatatatg cgttccctac aataattaca
     4261 ataccatgaa tatggttggg catcacaata aattcatcca agctaaaatt cttaaagtgt
     4321 tttggtattt ccacccaaaa attcttagta atctctccaa acttgttcaa aatcattttg
     4381 gcactctaga tttgcccaaa ccattcttcc ttattttttg tgcatagggt gacaaaatag
     4441 cacccgcttt gagaataatc ataatttttt aatctgttta attttctttc tctgtgactg
     4501 tatttaatgt ttattgcctt aattctccat tcgcaatttg tatattatat cgcttttatc
     4561 cagactttaa acaaattctc ctaagtttga accgctagct ctattgggtt agactttcga
     4621 catggcaaag aagcgaataa cgaaggttta tacgaggact ggagataaag gacagacatc
     4681 gctagtggga ggtaaaaggg ttagtaaatc ctctgctcga gtcgaggcct atggtgatat
     4741 tgatgagctt aattcagttc ttggaatcgt tcgggtagag gctcaagata acgaaattaa
     4801 cgaaataaca agagaaatcc agaatgacct ttttattata ggagcggatt tggcaagtcc
     4861 ttctgatatt caagttccta gaataagtaa agagagcata aaagggcttg aaaagatcat
     4921 agataaattt cttcaagagc ttgagccact taaagagttt atacttccta gcgggagggg
     4981 gggaggtcct tatcttcacc ttgcaagaac ggtatcaagg agggcggaaa gaagaatcgt
     5041 aaggcttata gaggaagaac aggtgaacga aaacatactt gcctatgtaa accgtctctc
     5101 ggatctgctt tttgtgatgg ctaggattga gaataagcat ggaaacttcg aggaaacata
     5161 tgtgagattc aataagtgaa gaattaaatt ggctatcagt tatcagtggt cagttgtcag
     5221 taaaagattt tgctgataac tgacggctaa tggctactac gctgacgaac aagatgaaaa
     5281 aaattgagac acaaacctac atacttaatc ctaaaattga agaataccta agaaacctca
     5341 ccccggttca ggatgaggta ttaaagaaga tggaagggtt aggaaacgaa agagggtttc
     5401 caataatcgg gccgcttata ggaaggctgc tttatcaact agctgtcatg acaggggcaa
     5461 aaagggtttt tgatatgggc tcagggtttg gttactcagc ctattggttt gctaaggcgg
     5521 taagggacga aggacttgtt gtgtttgcag agctttctca aaagaatgcc agtcttgcaa
     5581 aagagttttt tagacgtggt ggtcttgaca atagagtaga aatacatgtt ggagatgcac
     5641 ttcagatctt ggaagaaact gatggt
//
LOCUS       DATDRL010000002        12083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1579171, whole genome shotgun sequence.
ACCESSION   DATDRL010000002 DATDRL010000000
VERSION     DATDRL010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12083
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579171"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VI935_00040"
     CDS             complement(<1..754)
                     /locus_tag="VI935_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HLE24016.1"
                     /translation="MPKLTIDGQEADVLDGLNIIQAAALIGIEVPHFCYHPALSIVAQ
                     CRQCLVEIEGVPKVMPACSTFVRDGLVVKTNTTKAIKAREAVVEFTLINHPLDCPICD
                     KGGECPLQLTAFKHGPGYSRFDGPEEKKVRKKYFLSEKIMYDPNRCIMCTRCVRFTDE
                     VTQTGELGYDGRGFRKKIVVFPGKNLDNELSGNVIDLCPVGALLSKDTLHEERVWYWK
                     YTDTICPLCSNGCNITVGIDPRKGRVARVRPRV"
     gene            complement(1052..1462)
                     /locus_tag="VI935_00045"
     CDS             complement(1052..1462)
                     /locus_tag="VI935_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLE24017.1"
                     /translation="MERWFGMALLVVLLWGLGSLFGKLALIRDSPYRVYLFEGIGTLA
                     VLILFVFFRKHDTFANFSFNFYALLMGLTWGLGTIFFIIALQPAKLSVIVPLTALYPA
                     ITVLLSLLFLHERLELKEAIGIVLAILSIVLLSK"
     gene            2178..3170
                     /locus_tag="VI935_00050"
     CDS             2178..3170
                     /locus_tag="VI935_00050"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLE24018.1"
                     /translation="MSTMLSERLAPVERLKSGLNGTLKFIYLQATGRPRLVNLEVTKL
                     CNARCDFCDYWETKHEIRLSDYRPIIKKINPLVVSITGGEPMLRRDLSQIVRQIKECS
                     IFIFTTMVTKGDLLTIAKAQELLNSGLDQISISLDFLGKKHDAYRGIPGLWDQLSSLI
                     PQLSKISSNIVLNTIIMEDNLDQIIDISHQAKDWGVKVSYSSYSVMKTNNNSHFINGQ
                     LERVKKIVEELIELRRKWKGVIVSTEYYLKEIPGYFANGGIPDCLAGLNMITVTPSGH
                     MKRCSEMPVIAHGSEYYPGIFDKTRCTSCWYSCRGESQAPFTFKRALECLGIWV"
     gene            complement(3447..4883)
                     /gene="cysS"
                     /locus_tag="VI935_00055"
     CDS             complement(3447..4883)
                     /gene="cysS"
                     /locus_tag="VI935_00055"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HLE24019.1"
                     /translation="MENKIKVYNTLSQKKEDLIPFEGKKVRMYVCGPTVYSSAHLGHA
                     RAAITFDVITRFLRKVGYKVKYARNYTDVDDKIISKSKETGIPPEEVARIYTEEYKND
                     MFNLGVETPTYEPKVTEHIAEIIDLIKTIIDKGYAYQSDGDVFFSIKMFRGYGKLSKR
                     TPDEMLAGIRVDINEKKENPLDFALWKGVKPGEPWWESPWGKGRPGWHIECSAMSMRY
                     LGKSFEIHGGGKDLIFPHHENEIAQSEAATGVEFVKYWVHNGVIQINREKMSKSLGNI
                     INVKDALSIWSREAIRLFLLSHHYQNPADFSERALDESETALERLYIMLKRANDLTKN
                     NKREDKELATSLNLFNTEWIESMCDDFNTADTIGQLFDLVRATNRSIDAFGWTPTLQE
                     TIEEIRNFGSTLGILELDPNDYLKKEKLSKKSLGIDENEINELVEERTAARKEKNWKR
                     ADEIRNYLDSRGITLEDTSKGTIWRIKS"
     gene            complement(5111..5923)
                     /locus_tag="VI935_00060"
     CDS             complement(5111..5923)
                     /locus_tag="VI935_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLE24020.1"
                     /translation="MSVQNSVIKGNWALILGASSGFGEAISLKLAEAGMHIFGIHLDR
                     KSTIANVERVISQIKEMGGQVAFFNINAADPVKRKETVDHIESVLKENENASVIKVLV
                     HSLAFGTLKPYISSSQNEIVTESNMNMTLDVMAHSLVYWVQELVLRKLMVSGGRIFAM
                     TSAGGHRVWPTYGPVSAAKAALEAHIRQLAFELASLGITANAIRAGVTKTPALHRIPG
                     SETIIKKALEANPSGRLTTPKDVAEAIVALSQSGTHWITGNIIGVDGGEDLI"
     gene            6100..6969
                     /locus_tag="VI935_00065"
     CDS             6100..6969
                     /locus_tag="VI935_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24021.1"
                     /translation="MTILCSPCAQWLPFIFGFSLTMVDFNSELVYQFFVAQSLLIVYE
                     PFDSFVENKGYWKEYITFYERYWGGPTSPYGVWFGKEYETFREALTNYLGIDDHEIED
                     CFFMKDKDGRYYICPLTPKINPYLLCSEYIIPAEWFILFKDNERKFFNTPWGFAGIHY
                     DTKINLSLERIKEVEEILRNISTANKKNEPKLALFQKLNEIQARILELKNWLSGFDSS
                     SYVLLNYGEICSFIHPYTLKNERSVKEVWQLIYFLNEGRFNEAQSVLNLLTEKWDDIR
                     RKASGEISKLTVQ"
     gene            7350..8561
                     /locus_tag="VI935_00070"
     CDS             7350..8561
                     /locus_tag="VI935_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLE24022.1"
                     /translation="MINNTEFEALVDEYSSVQGGYIEDEEISPTIMSDEGFEDEFNLD
                     EEDARGVKLPGKTEEIQNHDLRLLNDYFKEIGSLSLLTAREEVEVAAKIRKCETRAKE
                     IKMIIERIIGKKIGGDIRSTVQGLKEVSDKTPNKSKKERVFKRLGRLLTLFDAYSKKA
                     TQFRNRFITANLRLVASIAKRYIGRRLPFMDLIQEGNLGLIKAVERFDYTRGYKFSTY
                     ACWWIHQAISRAIFDQTRTIRVPTYVLERSGKVKNVRSGLEKKTGRNPLPEEIAKKVN
                     MPVEGVKRVLGADERLAYLDSPIWQGEKMTLMDLVPDLNSPPPDLLIAAASIPKNLDE
                     ALMKLRPREREVLKMRFGIGYDNTSTLDEVGLHLGLTRERIRQIEKRALERLGKSKFA
                     PALRSLIEAYR"
     gene            8760..9200
                     /locus_tag="VI935_00075"
     CDS             8760..9200
                     /locus_tag="VI935_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24023.1"
                     /translation="MVIKIFKWKFDEGVFEGFESYLSGIGKDIDSLSEEDVEEFLKGF
                     NSEVSDTMREAFLNIMARRGLSKLISFGVEFDYKCDGCGEMINSSHGVELELDEVSYL
                     VCETCSQVIMKVKEHLDMGKTLEEASKIAEELSGGRTQIVVHQE"
     gene            complement(9384..9716)
                     /locus_tag="VI935_00080"
     CDS             complement(9384..9716)
                     /locus_tag="VI935_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HLE24024.1"
                     /translation="MGTYIMLSKLTPEGRKTIKERPGRIKEVDQELEAMGLKVIKQYA
                     TLGPYDFVNIVEASDNETVGKVSVDLCSRGTIEIMSLPAIPVEKFISSLKTVKRVKPK
                     KTTKAKRR"
     gene            9914..11272
                     /locus_tag="VI935_00085"
     CDS             9914..11272
                     /locus_tag="VI935_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24025.1"
                     /translation="MQYLISIILNTYTFSRPFILFFALICLISLDNSSVDAQSKAPGS
                     PDKEIVVKDYKWGSGGAGTVGIIREITLENKGKTAYKDIHIEVDLYTASDVPLGSLRS
                     TIHEELSGGSQKTFYNVNLGFMHSDLQKSVIRVVGAKPVDTSGQPKDLIIVKKWEWTG
                     GLYSTEGILKEITLENKSGTNYKNIEIQLDYLSGGGTKLGPTRAVIHDVLPAKGEKTF
                     YGINVGFRHPETNKTLVTVIDAERAPVKKPKPSVAKKGGVLDKYKKKVRPGDKTTSLE
                     TSEQTQVAHLSEKTGTAKTPQTRLALGVTKGAPQANDIKEPYDKGSLAEKEIGSPPYQ
                     REEAPDLGVPEEEYEEEPIPQDDIVVKDFKWGGNVTGTLGTFRELILENKSGITYTKI
                     ELMVDFYGVTERRPLGSYRVTIYDVLPPNSEKVFRDVKVGYVNVIPEDIEVRIIGALA
                     VR"
     gene            11623..>12083
                     /locus_tag="VI935_00090"
     CDS             11623..>12083
                     /locus_tag="VI935_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HLE24026.1"
                     /translation="MIFDTIMGWFSNDLAVDLGTANTLVYVKGKGIVANEPSVVAIQE
                     DGRGAKRILAVGKEAKEMVGRTPGSIRAIRPLKEGVIADFEVAQKMIGYFIRRTHNNR
                     KSFVRPRIIVSVPIGITEVEKRAVKESAEAAGAREVYLIEESMAAALGAGMP"
BASE COUNT         3572 a         2253 c         2546 g         3712 t
ORIGIN      
        1 ttacccttgg gcgtaccctt gccactctac cttttctcgg gtcaatccca accgtgatat
       61 tacaaccgtt gctacaaaga gggcatattg tatctgtgta cttccagtac caaacacgct
      121 cttcatgaag ggtatcctta ctgagcagcg cgccaactgg acaaaggtca atcacgtttc
      181 ctgaaagctc attgtcaagg tttttgccag gaaaaacaac gatctttttt ctaaatccac
      241 gcccatcata tccgagctcg ccggtttgtg taacctcatc cgtaaacctc acacatcttg
      301 tacacattat gcagcgattt gggtcataca ttattttttc gctaaggaag tatttctttc
      361 taaccttttt ctcctctgga ccatcaaatc tgctgtagcc tgggccgtgt ttaaaagcag
      421 taagctgaag ggggcactca ccccctttat cacaaattgg acaatcaagt ggatggttta
      481 tgagggtaaa ctccactact gcttctcttg ctttgattgc tttagtagtg ttggttttaa
      541 caactagacc atctctaaca aaggtgctac aggcaggcat tacttttgga acaccctcaa
      601 tctctaccag gcactgtctg cattgagcga cgatgcttaa agcggggtga taacagaaat
      661 gaggtacttc aatccctatt agtgcagcag cctgaataat gtttaaacca tccagaacgt
      721 ctgcttcctg tccatctatt gtcagcttgg gcatctatag actctttatt ttaaaaattg
      781 tatctgacct aagttttatc ttcaaggaag gttatttgtt tatttgattt gataatactc
      841 tttagtttgc tacaggtttc cgctgtcatt gcaatgacct aaagatactt tttatggaag
      901 gctaaagcct tcccctacaa tttttgtttt atggtgaatc caaaatgaag cggagaccct
      961 cgggtctcca gatgaagatc gaaacgatgg ccccttcctc ttatgtgctg gtacccttgc
     1021 tgctgggttc ttcatttggt aatttagatt ttcattttga taacaagact attgaaagaa
     1081 tagctaaaac aattccgata gcctctttta gctctaacct ttcatgtaaa aagaggagag
     1141 aaagcagaac agtgattgca gggtaaagtg cagttaaagg tactattaca gaaagctttg
     1201 caggctgtaa agcaataata aagaatattg tccctaagcc ccaagttaaa cccattagaa
     1261 gagcatagaa attgaaagaa aaattggcaa aggtgtcatg ttttctaaaa aacacaaaga
     1321 gtattaaaac agctagtgtc ccaatccctt caaataggta taccctgtag ggtgaatctc
     1381 ttatcaatgc cagttttcca aaaaggcttc ccaaacccca caggaggact acaagaagag
     1441 ccattccaaa ccatcgttcc atttatctcc tctattgaat tttgtagatt tataacttta
     1501 atctgaagca cggcaaccag atgattctaa aacaaatgag gttttatatc aaacctatgc
     1561 atgtgctatc cggcttaatg agcaagagcg gggggaggat tttgcctccc tcaggcttgg
     1621 tatgtacgta caacgttgga tcatgtgggt cagggctggg tttaccggta gcacatcgca
     1681 tataggttcg tgtgcatagt tgattccaga atttttacac tataaataag acgaaatatt
     1741 aaacgggttg atttaagttg aatgatacca cttccaataa agaaatgacc aataactgtt
     1801 gtaggcacga acttgttcgt gctttaaaat agcacagaca agcctgtgcc tacaagtatt
     1861 tgaacttatt aattggaaat ggtatgagtt atagcggcct ttttgtcgga ggatgtcagg
     1921 tggggttaag cgaaaccctt ttcggtgtaa tattttgcga ctcatttgaa tgctaagtgt
     1981 acggtgttcc ggtcggttga tttaaagatt aggcttatag ctcaacatca tcgacgggac
     2041 gttctagtcc cgcggttaaa ttgtggctgg aagccgctct tacggttttt ttcctgacat
     2101 tggcatgtat aaatactatt cgtaaatcta aaattagcta aaattccagg cttgactctt
     2161 ttttgataat acatataatg tcaacaatgc tctccgaaag attagcgcca gttgagagat
     2221 taaaaagcgg gttaaatggc acccttaaat tcatctacct ccaagctaca gggcgtccaa
     2281 gacttgttaa ccttgaagta actaaactct gtaatgctcg atgtgatttt tgtgactact
     2341 gggaaacaaa acacgagatc cgcctttctg actatcgtcc gataataaaa aaaataaacc
     2401 ccttggtagt atcaataact ggcggagagc cgatgctaag gcgcgatctt tcgcaaatag
     2461 taagacagat aaaagagtgc tctatattta ttttcacgac tatggttaca aaaggcgacc
     2521 tactaacaat tgcgaaggct caggaactcc taaattctgg gcttgaccaa atatctatct
     2581 cgctcgattt ccttgggaaa aagcatgatg cctatagagg tattccgggg ctctgggatc
     2641 agttgtcaag cctcattcct caactgagca agatatccag taatattgtt cttaacacaa
     2701 ttataatgga agacaacctt gaccagatta tagatatttc tcaccaagca aaagattggg
     2761 gtgtaaaggt atcctatagt tcttattctg tgatgaaaac caacaacaat agtcacttta
     2821 taaacgggca acttgaaagg gttaaaaaaa tcgttgagga gctaatagag cttagaagga
     2881 agtggaaagg cgttattgta tctactgaat attatttaaa agaaattccc ggatattttg
     2941 cgaatggtgg cattccggat tgcctagctg ggttaaacat gatcactgta accccaagcg
     3001 ggcacatgaa gcgctgttct gaaatgccag taattgctca cggtagcgaa tactatccgg
     3061 ggattttcga taaaactaga tgcacatctt gctggtatag ctgtcgtggt gaaagccaag
     3121 ccccctttac ctttaaaagg gctcttgaat gccttggaat ttgggtttag gttggtagct
     3181 atttacccaa taataaacac ggatttacac gaaaatatgt tactaactgg cgttaagcac
     3241 ttatgcgctt aagggcaaga ggttatcatt cccgcatgta tttagcggga atccaaatac
     3301 ctggcagttc atggatgccc gataaaagca ttcagtcatg acaagcaccc tagtgagatt
     3361 gctgaatcca gtaaaaattg cgtaacatga gtcaataagc cttttcctcc gtgatcattt
     3421 gtgtcaattt gtggattaat tgttatttag cttttaatcc tccaaatggt gccttttgaa
     3481 gtgtcttcca gtgttattcc cctagaatct agataatttc taatttcatc agcgcgcttc
     3541 cagttttttt cttttcttgc ggcagttctc tcttctacca actcgtttat ttcattctca
     3601 tcaatcccga gagatttctt acttagtttc tccttcttaa ggtaatcgtt tgggtctagt
     3661 tctaggattc ctaaggtact tccaaaattt cggatttctt caatggtttc ttgtaaagtg
     3721 ggagtccaac caaaagcatc aattgatcta tttgttgctc ttaccaaatc aaagagttgg
     3781 ccgatggtgt cagcagtgtt gaagtcatca cacattgatt cgatccattc tgtgttaaaa
     3841 agatttaagc tggttgcgag ttctttatcc tctcttttgt tgttctttgt caaatcgttt
     3901 gcacgcttaa gcattatgta tagcctttct agagctgtct ctgattcatc caatgccctt
     3961 tctgaaaaat cagcagggtt ttgataatga tgagaaagca ggaaaagacg aatcgcttct
     4021 ctactccata tagaaagtgc atctttaaca tttataatgt taccaaggga tttcgacatc
     4081 ttttcccgat ttatctgaat aaccccgtta tgaacccagt actttacgaa ttcaaccccg
     4141 gttgcagctt ctgattgggc aatctcattt tcatgatggg gaaagattaa atccttacca
     4201 cctccatgga tctcaaagct ctttccaaga tacctcatgc tcattgcaga acactcgata
     4261 tgccatccgg gtcgtccctt tccccatggg ctttcccacc atggttcacc aggctttacg
     4321 cctttccata gcgcaaagtc aagtgggttt tctttcttct catttatatc aacccttatt
     4381 cctgcaagca tttcatcagg tgttctcttt gagagctttc cgtatccacg aaacatcttt
     4441 attgagaaga aaacgtctcc gtcagactga tatgcgtatc ctttgtcaat aattgttttt
     4501 atcaggtcaa ttatctcagc tatgtgttcg gttacctttg gttcatatgt aggagtttca
     4561 actcctagat taaacatatc gtttttatat tcctcggtgt atattcgtgc tacctcttca
     4621 ggtgggattc cggtctcttt ggatttgctt ataatcttgt catcaacgtc tgtgtagttt
     4681 ctcgcatact ttactttgta gccgaccttt cttaaaaatc ttgtaattac atcaaatgta
     4741 atagcagccc ttgcatgacc aagatgtgcg gaactataaa ctgtgggacc gcagacatac
     4801 attctgactt tcttgccttc gaagggaatt aggtcttctt ttttttgtga aagggtgtta
     4861 tagaccttta ttttattttc cattgccagc ttccatgttt atttagttct cttatatcct
     4921 tccatatgta ctttttggcc actaatgaac cattgtagaa gcgccatcct tggcgcggct
     4981 ggattgcggc tggaagccgc tcttacgctt gtccttacgt ctaattgaca atgactaacg
     5041 acgaatagcc aatttatttt cttttgtttc gtgtagattt ttgctaattc gtggctgaat
     5101 aactctttta ttaaatcaga tcctctcccc catctactcc aataatgttt cctgtgatcc
     5161 agtgcgtgcc actttgagaa agtgcaacta tagcctctgc tacgtctttt ggcgttgtta
     5221 ggcgcccgct tgggttagct tcaagtgctt tttttattat agtttcactg ccaggaattc
     5281 tatgtaaagc aggcgtcttc gttacacccg cccttatggc atttgctgtt attcctaggg
     5341 aggctagctc aaatgcaagt tgtcttatgt gggcttcaag tgcggcctta gcagcggaaa
     5401 ctggaccata ggttggccaa accctatgtc cgccagcgct tgtcattgcg aatatccttc
     5461 cgcctgagac cataagtttt cttaaaacta attcttgaac ccagtaaacg aggctatgtg
     5521 ccataacgtc tagagtcata ttcatgttgg actcagttac gatctcattc tgtgaagatg
     5581 aaatgtaagg ctttagggtt ccaaaggcta aggagtgaac tagtactttt atcaccgaag
     5641 cattttcgtt ttccttaaga actgattcga tgtgatctac cgtttccttg cgtttcacag
     5701 ggtcggcagc attaatatta aagaaagcga cctgtcctcc catttcttta atttgactaa
     5761 taaccctttc cacattagct attgtggatt ttctatctag atgtattcca aatatatgca
     5821 ttccagcttc tgcaagctta aggcttatcg cttcaccaaa accgctcgaa gccccaagta
     5881 tcaatgccca gttacctttt atcacgctat tttgaacact caaaaccctt cctcctttga
     5941 aaacaaacac tagagattct acgcagtttg agattaattt caaggaaacc atatttagcc
     6001 acagagggca cagagtagtt agtcgctagc tattaatcgt tgggcgtttg attatcgtcc
     6061 gattctgagc ccacgatgaa ttctaatgac cgatgactaa tgactattct ctgttctcct
     6121 tgtgctcagt ggcttccttt catctttggt tttagtctga cgatggttga ctttaattct
     6181 gaattggttt atcaattctt tgtggctcaa agtcttctca tagtttacga accatttgat
     6241 agttttgtag aaaacaaagg gtactggaag gagtatatca ccttttacga aaggtactgg
     6301 ggtggcccaa cctcacccta tggagtttgg tttggcaagg aatatgagac ttttagggaa
     6361 gcattaacga attatcttgg aattgatgat catgaaatcg aggactgctt ctttatgaaa
     6421 gataaagacg gaagatatta tatctgtcct cttaccccaa aaattaaccc ctatcttttg
     6481 tgctcggagt acattatacc agcggaatgg tttatcctat ttaaagacaa tgagaggaag
     6541 ttctttaaca ctccgtgggg atttgctggg attcattatg atacaaaaat aaatttaagc
     6601 ctcgagcgca taaaagaagt agaggaaatt ctgagaaata tctcaactgc aaataaaaag
     6661 aatgaaccaa aattagcttt attccaaaag ctaaatgaga ttcaggctcg cattttagag
     6721 ctcaaaaact ggctatcagg gtttgactca tcaagttatg tacttctaaa ttatggggag
     6781 atatgttctt tcatacatcc ctatacctta aagaatgagc gttctgtaaa ggaggtttgg
     6841 cagttaatct actttctaaa cgaaggaagg tttaacgaag ctcaatcggt gcttaatttg
     6901 ctaacagaga aatgggatga tataagaaga aaggctagtg gcgagattag taagttaacc
     6961 gttcagtaat taagggtaat acaccacaag gtcgcactaa aattctagaa tataacaaca
     7021 cttgtaaagg gggattaagc gaggatttaa tttgattttc ccattttgtg agagggagga
     7081 agaagattct tataagtaaa ttcagaaaat aataaaaatg attaaacagc ttgtaatatc
     7141 aaagcaatcc atgcccaatt aactaaatta tactatttat aggcgttata aaggataaat
     7201 tattgaatct ttcgtgaaaa ttatgagtca aactgtaact ttgaataatg tgaaacctat
     7261 ttcttcttaa ctggttaaat ataataatgc ttcttactac ttcctgcatc catttatagg
     7321 aattttgcat cttaatggag agcaaagtca tgataaacaa tacagaattt gaggctcttg
     7381 ttgacgaata tagttcagtt caaggtggtt atattgaaga tgaagaaatc tcccccacaa
     7441 taatgtccga tgaaggtttt gaagatgaat ttaatctcga tgaagaagac gccagagggg
     7501 tcaaactccc tggcaaaact gaggagatcc agaatcatga cctaagactt ttaaacgact
     7561 actttaaaga aatcgggtct ctttctttgc ttaccgcgag ggaagaggtt gaggtcgccg
     7621 caaaaataag aaaatgtgag acaagagcaa aagaaataaa aatgattatc gaaagaatta
     7681 ttggaaagaa gatcggaggt gatataagat caactgtgca ggggttaaag gaggtttctg
     7741 ataagacccc caataagtcg aaaaaggaaa gggtcttcaa acgccttggc aggctattaa
     7801 cactctttga cgcatattca aagaaggcga ctcagtttag aaacaggttc ataactgcga
     7861 acctgaggct ggttgcgagt atagcgaaac ggtacatagg tcgcaggctt ccttttatgg
     7921 atttgattca agaagggaat ctcggactaa ttaaggctgt agaaaggttt gattacacaa
     7981 gggggtataa gttttcaaca tatgcatgct ggtggattca tcaagctata tcgagggcga
     8041 tctttgatca aacaaggaca ataagggttc caacctatgt actcgaaaga tcaggtaagg
     8101 taaagaacgt acgttctggg cttgagaaaa aaactggaag aaatccgctc cctgaggaga
     8161 ttgcaaaaaa ggtgaatatg cctgtagagg gtgtaaagcg tgtacttgga gctgatgaaa
     8221 ggttggcata tcttgattct cctatatggc agggtgagaa gatgaccctt atggatcttg
     8281 ttcccgacct taactcaccg ccacccgatt tactcatagc ggctgcttcc attccaaaaa
     8341 accttgatga agcactcatg aagctccggc ctagggaaag agaggttctc aaaatgaggt
     8401 ttggaatcgg ttatgataat acatctactc tagatgaggt cggattgcat ttaggtctaa
     8461 ctagagagag gattaggcag atagaaaaac gggctcttga aagactaggg aaatcgaaat
     8521 ttgcccctgc actaagaagt ttaattgaag catatcgttg agtaatctag tatggtttcc
     8581 atctgatcta acccagggtt ttacgccgct tctatttttt ctgacaacca atcatgcaat
     8641 tcgtctggat tttttatttt taacctagct aactatgtga agctcaaaat atttgccttt
     8701 ctatatattg ctattgtatc atatcacacc tcgtttttta tattagcagg ggggacaata
     8761 tggttattaa gatttttaaa tggaaatttg atgaaggcgt cttcgagggt tttgagagct
     8821 atttatcagg gattggaaaa gatatagatt cccttagcga agaggacgtt gaggaattct
     8881 tgaaaggatt caattctgag gtctcagata caatgagaga ggctttctta aatattatgg
     8941 ctagaagagg actcagtaaa ctgatttctt tcggagttga atttgattac aaatgcgatg
     9001 gttgtgggga aatgataaat tcctcacatg gtgttgagtt ggaactcgat gaggtatcat
     9061 accttgtgtg tgaaacttgc tcgcaggtta ttatgaaggt aaaagagcac ttagatatgg
     9121 gaaagacgct tgaagaggct tcgaaaatcg cagaagaact ctctggggga aggactcaga
     9181 tagttgttca tcaagaatga taggggtttt cttcggaatg gagtgaatgg tgtgtagaat
     9241 aagctataat gaattttctc atgcttacac aaatcttagt ttacgcccaa ttcgtgatta
     9301 aatggatcgg agtggaagag gaacaaaaac ctaaaaccac aaatatagag tgacgataat
     9361 tatagttatc gaagtctctt tatttatctt cttttagctt tagtagtttt tttgggttta
     9421 acccttttca ctgttttaag cgatgatata aatttttcta cagggattgc aggcaatgac
     9481 ataatctcta ttgttcctcg agagcacaaa tccacagaga ccttgccgac tgtttcgttg
     9541 tctgaggctt ctacaatgtt tacaaaatcg tatggaccaa gggtagcgta ttgtttaata
     9601 accttcaaac ccatggcttc aagctcttgg tccacttctt taattcttcc cggtctctct
     9661 tttatggttt ttcttccctc tggtgtaagt ttgcttaaca taatataggt tcccataatt
     9721 taaccccctc cttatttaat gacattaatc atatttatat catttgttag ggtcataatt
     9781 caagtggttt ttttcttttc catcacaatt ctcttccatc cattattgaa aagaaggcgg
     9841 attttaaaat atatgaggct gccatttcag gttttgttgt gtataatcta ttttagttaa
     9901 gttggagaga gatatgcaat atctaataag cattattcta aatacgtata cgttttcgcg
     9961 gccatttatc ttattttttg ctctcatatg tttaattagc cttgataatt catcggtcga
    10021 tgctcaatct aaagctcctg ggtctccaga taaggaaata gtggtaaagg attataaatg
    10081 gggttcgggt ggtgcgggta cagttggaat tatcagggag attacattag aaaacaaggg
    10141 taagacggca tataaggata ttcacataga ggttgatctc tacaccgcaa gcgacgtacc
    10201 gcttggttcc ttgagatcaa cgattcatga agagctttct ggcggtagtc agaagacctt
    10261 ttataatgtt aatcttgggt ttatgcactc tgatctacag aaatccgtga taagggttgt
    10321 gggagcaaaa cctgtagata cctcaggtca accaaaagat ttaataatag taaaaaaatg
    10381 ggagtggaca gggggcctct actctactga ggggatacta aaagaaatca ctctcgagaa
    10441 taaaagcggg accaattata aaaacataga gatacaactg gattacctca gtggaggcgg
    10501 cacaaagcta ggtcccacta gggctgtaat ccatgatgtt cttcccgcaa aaggtgaaaa
    10561 gacattttat gggataaacg ttggattcag acaccctgaa acgaacaaaa ccttggtcac
    10621 tgttatagat gctgaacgcg cccctgttaa aaaacctaag ccaagcgtgg ctaagaaggg
    10681 tggtgtgtta gacaagtata agaagaaggt aagaccaggt gataaaacaa cttctcttga
    10741 aactagcgag caaactcaag tcgcccattt atccgaaaaa actggtactg caaaaactcc
    10801 tcagacgaga ttagcattgg gtgttacgaa aggggctcca caagctaatg atattaaaga
    10861 accttatgat aaaggttctt tagccgaaaa agagattggg tctccacctt atcaaaggga
    10921 agaggctcca gatttaggag ttccagaaga agagtacgag gaggagccaa ttcctcagga
    10981 tgatattgtt gttaaggatt ttaagtgggg gggcaatgtt accggaacgc tagggacttt
    11041 tcgtgaactc atacttgaga ataaaagtgg tattacttat acaaagatag agcttatggt
    11101 ggatttctac ggagtaactg agcgaagacc tcttggaagc tatagggtta cgatttacga
    11161 cgtccttcct cctaatagtg agaaggtatt tcgagatgta aaggttgggt atgtcaatgt
    11221 tattccagag gatatagagg taagaattat tggcgcttta gctgtaagat agtaattttt
    11281 gcgattatca attataaaaa tgttcttcac atcttcatga tttcgaggga caacagagta
    11341 gaattaatta agcctcggac aaattggtaa gggtgaagtc tatttgagaa caagtaagaa
    11401 ttagaaaact ctaaggttca acagatttta cctgaatctt gtttagtttt gattaaaaaa
    11461 tcaagaacac cgggttgaaa agggttaata aatattaggt tgcaacgtga gataccaact
    11521 gcgatcttca tttttctctc ttgattttat ctgccttctt tgatataggt ataaaatcgt
    11581 ataacattaa ccttgagaaa tgggaaggag ggttgactaa atatgatttt tgacactatc
    11641 atgggatggt tttctaatga cctagcagta gatttaggga ctgcaaacac actcgtatat
    11701 gtaaagggaa agggtatagt tgctaatgag ccttctgtgg ttgcgataca agaagatggg
    11761 aggggggcaa agagaatact agctgttggt aaggaagcaa aagaaatggt tgggcgcacc
    11821 cccgggtcaa ttagggctat taggccgtta aaagagggtg tgatagcgga ctttgaggta
    11881 gctcagaaga tgatagggta cttcattaga aggacacata acaatagaaa aagctttgta
    11941 aggcctagga taattgtttc agttcctata ggaatcactg aggtagagaa gagagcggta
    12001 aaggaatcgg cagaggctgc tggtgctagg gaggtgtatt taatagagga gagtatggcg
    12061 gctgcacttg gagcaggaat gcc
//
LOCUS       DATDRL010000003        13030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2631597, whole genome shotgun sequence.
ACCESSION   DATDRL010000003 DATDRL010000000
VERSION     DATDRL010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13030
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2631597"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..895
                     /locus_tag="VI935_00095"
     CDS             239..895
                     /locus_tag="VI935_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HLE24027.1"
                     /translation="MFGESSVIEFMRKGGFFMYPILLCSIVGLAIFLQKIWMLRVKRI
                     IPEQFLNTLYGFLSQGKISEAIVLSRTNESSTARIALAVLENSNKSKEELKEEIEAAG
                     RKEAQELGRYIEGLGAISNASTLLGLLGTISGMIKIFKVISEQTIVNPPSLAAGISEA
                     LYTTAFGLLVAIPAFIAYKYASGKAEGLITDMEGEAKRIMEEVLFANERKDKTREIAR
                     "
     gene            892..1320
                     /locus_tag="VI935_00100"
     CDS             892..1320
                     /locus_tag="VI935_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HLE24028.1"
                     /translation="MRFRKENGNKGGLGAGVDITPIVDTVFNLLIFFALSLNFAATSG
                     GINIKLPKASSAEPVKADQLTINLTKDGKMYLNDKVITSEKLSETLEKNQNKESLVII
                     RADSLVPHGRVVEVMDTVKTKGYSRLAIAVEHAPSQKEEQ"
     gene            1413..1964
                     /locus_tag="VI935_00105"
     CDS             1413..1964
                     /locus_tag="VI935_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HLE24029.1"
                     /translation="MILLIITFITSSSLAEEKKTLELRVFGICPTCSSDVKSKVLELP
                     GVHEAQVDIVKKKIRASYNPESVSETKIILDLRRGGYEVRRPFEKSRVERTALGISGI
                     KDKSDLIEIEWILYVYFDVDQVEISKISDNIVAIVNYKKGGGDPKQFVLSVSGILPEL
                     EIEVLKNLDTLKVELDKVKPILN"
     gene            complement(1992..2216)
                     /locus_tag="VI935_00110"
     CDS             complement(1992..2216)
                     /locus_tag="VI935_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24030.1"
                     /translation="MGTTITISPFTFYPECGCKVPLFGHEREEHDKMDHQPITYWGIY
                     MDGENISYTSTRELADKTKVWMEKWLEDRL"
     gene            complement(2462..2635)
                     /locus_tag="VI935_00115"
     CDS             complement(2462..2635)
                     /locus_tag="VI935_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HLE24031.1"
                     /translation="MSGKERRSATYGIDLNIESQGGENLRILFSINGEHVEEGGELKE
                     GDEVPFIPPVSGG"
     gene            2865..3362
                     /locus_tag="VI935_00120"
     CDS             2865..3362
                     /locus_tag="VI935_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HLE24032.1"
                     /translation="MSKDKIKKKTKKTKSKVITGLKSKDVIELIEALENIKLDFDHLH
                     YYERTGLIVPSIRKGQGRGIPKLYSVEDFIILRWLVSLQRNGISIQRFRDIIEFLKEK
                     IPDAIKKPQNYVLITDGTSIQFFDKVSAKALDVLKDTGQYLFVFPVGKIIEQSEKAIK
                     KIDKE"
     gene            3862..5130
                     /locus_tag="VI935_00125"
     CDS             3862..5130
                     /locus_tag="VI935_00125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLE24033.1"
                     /translation="MTDYNLDFLFIGGGILGLSGAMALSKRLEERNQSARIAVIDVDL
                     EGEHSSTLKNAGGVRATWKNHANIELCKYSINFYETIREEIQFRELGYYWMHDEAWWK
                     EIKKNHPLYKEYGLTVELYPGEAVPEILPFVDNLDAIAGLSISRNAGLVDHYSLREYY
                     RREAKKRGVKFFDRLYVKKIIVEDGRVTEIIAHQVEKPEGNIRDNSELIKTYLVNGEI
                     GYQTEAVSFKCDVLINTAGAWAPRISKLYGFNDEKIKPRRRQMVVIRCPEVDLSPYGV
                     VIDTSHVYFHKDAENILAGYSNMDEPYGYNLEFNFGGLDEGSPFVKYIWLPLWRRISK
                     FERIKLIRGWAGLYEETPDRSGYLGRVPGFKNVYECGAHTGRGLMISYGAGTALTDLI
                     LDGKFREELKHAKDLSRERLSGELFEELHL"
     gene            complement(5134..5442)
                     /locus_tag="VI935_00130"
     CDS             complement(5134..5442)
                     /locus_tag="VI935_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HLE24034.1"
                     /translation="MGNLVEVAKTGDLEPGQGMMVEVEGKNIALFNINGTYYAIDDTC
                     THVGGPLSEGEIQEHTVICPWHGAEFDLKTGEVLGPPAKNSVKSYKVHLEGNSIKIEV
                     "
     gene            5499..5735
                     /locus_tag="VI935_00135"
     CDS             5499..5735
                     /locus_tag="VI935_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24035.1"
                     /translation="MVDKSTHIIKDVYGVYAKALPDIQRHLLPGVLFACLRGHSIYVS
                     DVASRMKGVSIGAKEIKFLRLLHPQAIQSVWLGL"
     gene            complement(5732..5989)
                     /locus_tag="VI935_00140"
     CDS             complement(5732..5989)
                     /locus_tag="VI935_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24036.1"
                     /translation="MTVDTVLTKNRFFRLTTKLTPIKNGTRVSWYFAKLSGTNPFHTL
                     VSRWKTSKMKGMLTNIFTQGDVSLREMIETDLGAGKVIVQV"
     gene            complement(6149..6841)
                     /locus_tag="VI935_00145"
     CDS             complement(6149..6841)
                     /locus_tag="VI935_00145"
                     /inference="COORDINATES: protein motif:HMM:NF022307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HLE24037.1"
                     /translation="MSQKGYLVIADISGYTAFLTHAELDHAEGIMKRLIKTLLEQMKS
                     PFFVSKLEGDAIFVYAPEGSFVQGQTLLEAIENIYCLFAMTLESMFRDTTCRCKACEL
                     IPTLDLKFVLHHGTFVLSDIAGRQELSGPDVILEHNLLKNKINEKLGVKAYAFISRAF
                     ADAMSLGELTEGMKMHTGNYEHLGDVSGYIHDLHSVWARERERRRVYVDPKQAWFVVE
                     ADVPVQPALAWD"
     gene            7242..7604
                     /locus_tag="VI935_00150"
     CDS             7242..7604
                     /locus_tag="VI935_00150"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HLE24038.1"
                     /translation="MSRKKQSQQKTGANSNLFKVAKIGEVQSGKRKVVLVNGTEIALF
                     NINGEFHAINNRCPHAEFSLGAGLIYEDMVICPGHAWTFKLKTGECITNKSQPVKTYK
                     VVIEGKDIKIQLFNHFAT"
     gene            complement(7719..8480)
                     /locus_tag="VI935_00155"
     CDS             complement(7719..8480)
                     /locus_tag="VI935_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HLE24039.1"
                     /translation="MNEIKLLYKPENKPMSVAVLVSGTGTNLLALYEEQKRLERLGEK
                     NYGRIEVVFTNFPDCKGVERAREFGIPVISLSSKSFFDILQKGPDDNKARDFYDAAAI
                     TLIEEICYPDLVVLAGYRRRLGSIFIKRYKNRILNIYPGDITKTYLERGVEAPIQALR
                     AGENTIKCTIFLAREEERFGPAIIQSDQISLDGFKQEDINEIEEKVRKEGEWVIYPFA
                     VHHLIAKGRVGIDLEDNIYINGIRMPKEGYQFKIK"
     gene            8635..9513
                     /locus_tag="VI935_00160"
     CDS             8635..9513
                     /locus_tag="VI935_00160"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HLE24040.1"
                     /translation="MTMSNLTQRILTAVVGVPLLYFIFYLGGIPFLVLILAVIFLGEI
                     EFFKLLEVKGVQSERELAVAFSLALGIAAYFGYFYFAVVFSFSVIITFLLALRQQDLS
                     NTLIAISTTLFAIIYLGWFLSHAILLRNIDCNSNLKTYAETVQGLSDVGFFYIFLVVS
                     CTFLNDTGAYFIGRWKGKRKLIPHVSPGKTVEGTIGGIISSIITGGVANLIFKAPLEY
                     HWVILFGLVIGIVAVLGDLTESLIKRSAEAKDSGGILPGHGGILDRFDSLIFTFPVSY
                     YMVLLYYWLHGVLVSS"
     gene            9973..11052
                     /locus_tag="VI935_00165"
     CDS             9973..11052
                     /locus_tag="VI935_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HLE24041.1"
                     /translation="MGKEYTKFIAAAVQTAPVFLNKEETIGKACSLIEEARDNGADLI
                     VFPEAFIPTYPYWPKDLGTGPERKLILDAYTELYKNSVEIPSEDTDKLREAAKKAGAY
                     VIIGMNERDGGTLYNTILFIGKNGTILGKHRKLMSIDSEKCIWGMGDGSDLRVFDTEL
                     GRVGGFFCYEHHMTLAKYAMFAEGEQIHAGLWAGHGFVKPTMDFASRQYAFEGQVFVI
                     AASLYINEDMVPNSFPLKKYTKWDFPGGSGIINPRGEYLAGPLYDKEGILYSEIDMDI
                     IIRAKAVIDAMGHFSRPDILSLNINKEKYSLIKIKGSGVEASSNEHGEIKKEMKELRE
                     RYERLEAKIEELIKGSTTGYKRIRR"
     gene            complement(11554..11805)
                     /locus_tag="VI935_00170"
     CDS             complement(11554..11805)
                     /locus_tag="VI935_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HLE24042.1"
                     /translation="MASNIAEGFERKTNKDFVKFLYYAKGSTAEVRTQVHIAKNLSHL
                     SDEDFNEIVTQTNLLTAKIGKLISYLTYSTRKQANSPTR"
     gene            12205..12654
                     /locus_tag="VI935_00175"
     CDS             12205..12654
                     /locus_tag="VI935_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24043.1"
                     /translation="MAQKKGPKDPLSEFIRVFEQQRKEMEKLAKPFLNYQSQMEKMVK
                     PFLDYQKRSEKVAKPILEYHKKLLEETTRFQETFAQNIIETIGKLINQIIEEQRKQTE
                     ETNKLLSEVRLPNQATEYLHSIQKIQERWIEQLKKSTEMIESFVKTK"
BASE COUNT         4032 a         2402 c         2629 g         3967 t
ORIGIN      
        1 tgcaagcgga taggtgtcat tgcgaggcta acaaagtcag ccaaagcatt ctcaatgctc
       61 agggcaagct cagcaatatc tttatttcag aggtattaga ttgaacgcca tgttttgtaa
      121 ccatttctca cacgcttttt atttcccttt gaaccacact gcggttcaaa gaatgttgcg
      181 cttgaaatac tgcaagtggc tcgcaataac tttataggaa tctacaagga ggcttctaat
      241 gtttggagag agcagtgtaa ttgagtttat gagaaagggt gggtttttta tgtaccccat
      301 tcttctctgc tcgattgttg ggcttgcaat atttctccag aaaatatgga tgcttagggt
      361 taaaagaata atccctgagc aatttctcaa cacactttat ggatttcttt ctcagggaaa
      421 aataagtgaa gctatagtgc tttctcgcac aaatgaatct tcaactgcga ggattgcgct
      481 tgctgtactt gagaattcga acaaaagcaa ggaagaactc aaagaagaaa tagaggcagc
      541 gggtcgaaaa gaagcacaag agctcggacg ttacatagag ggacttggtg cgataagcaa
      601 cgctagcact cttcttggac tcctaggaac aatctctgga atgataaaaa tattcaaagt
      661 catatccgaa cagacaatag tcaatccgcc cagcctagcg gcaggaatat ctgaggctct
      721 ttacacaaca gcatttgggc tattggttgc tattcctgcc ttcatagcat ataaatacgc
      781 ctcgggtaaa gcggaaggac ttataacgga tatggaggga gaggctaaaa gaataatgga
      841 agaagttctt tttgccaatg aaaggaagga taaaactagg gagattgcaa gatgagattc
      901 agaaaggaaa atgggaacaa aggcggatta ggtgcagggg ttgatattac accgattgta
      961 gatacagttt ttaacctcct catatttttt gcactctctc ttaatttcgc tgcaacttca
     1021 ggtggaataa acataaagct accgaaagca agttcagccg aacctgtaaa agctgatcag
     1081 cttactatta atcttactaa ggacggaaag atgtacctta atgataaggt aatcacttca
     1141 gaaaagcttt ctgaaacact agagaaaaat caaaacaaag agtcacttgt aataatacgg
     1201 gctgatagcc ttgttccaca cggaagggtc gttgaagtaa tggatactgt aaaaacaaag
     1261 gggtattccc gacttgcaat agccgtagag catgctccgt cacagaagga agaacagtaa
     1321 ctataagtat attaccaacc tttgaacctt ttcccataaa aatcgtctaa aagctacaaa
     1381 gtggtaagtc aagaataaga gcttttgtcg cagtgatatt attaataatt actttcatta
     1441 cgtcttcttc attagcggaa gaaaagaaaa cactagaact cagggttttt ggaatttgtc
     1501 caacgtgtag tagtgatgtt aaatcaaaag tcttagaact tcctggcgtc catgaagctc
     1561 aggttgacat agtaaaaaag aaaattcgtg caagctacaa tccagaatct gtaagtgaaa
     1621 caaaaataat attagacctc agacgaggcg gatacgaggt gaggcgtccg tttgaaaaat
     1681 caagggtaga aaggacagct cttggaatat ctggaatcaa ggataaaagc gatttaatcg
     1741 agattgaatg gattctctac gtctacttcg atgtagatca ggttgaaata tccaagatct
     1801 cagataatat cgtcgcaatc gtaaattata aaaaaggtgg aggtgaccca aaacagttcg
     1861 ttttatctgt aagcggaatt ttgccggagc tagaaattga agtacttaaa aacttagaca
     1921 cgcttaaggt tgagttagat aaagtaaaac ctatactgaa ctaagcatgt ttaaactcac
     1981 tgctatgctg attaaagcct gtcctctaac catttctcca tccaaacttt tgtcttatca
     2041 gccaattctc tggtagatgt gtaagagatg ttttcaccgt ccatataaat accccaatat
     2101 gtaattggtt gatgatccat tttatcgtgc tcttcccttt catgcccaaa tagaggcact
     2161 ttgcagccgc actctgggta aaatgtaaaa ggagaaattg ttatcgtcgt tcccatttta
     2221 gctacgtccc atttctaact aatctgttta cacagtgttt ataagttaat catttattgg
     2281 gtgttttcaa gggattagga aggtaaaaag tcattttcca tgttaattcc cataaatatc
     2341 ttaaataact cgttatcgaa tttatattag ggggattgtt agttcattcg gttttcttaa
     2401 caaaagacga ggaaaaccgt ttgaataata taagggcgaa atagttttat tacatagtta
     2461 attatccacc acttacaggc gggataaaag gtacctcatc gccttcctta agctctccac
     2521 cctcttctac atgctctccg tttattgaaa agagaatacg aagattctca ccaccctggg
     2581 attctatgtt taaatctatt ccataggtcg cggaccttcg ttcctttccg gatattcatt
     2641 actttctctt tagacccatc cccagagttt ctttcaaata tgcaaatagc gatattctga
     2701 ctttcatcat ttaatgggaa ataatataca caatgagtat ataagttaat aaattgatgt
     2761 gtgaaattcg aaacagattg aacgacttta gtgtttgtgt tattttggag ttaaaattgg
     2821 ttactttata aactcacccc tttttacaca ttaacttgga ataaatgagc aaggataaga
     2881 tcaaaaagaa gacaaaaaaa acaaaatcaa aggtcataac tggtttaaaa agcaaggacg
     2941 ttattgaact aatagaagcc cttgagaata tcaagctgga tttcgatcat ctacactact
     3001 atgaacgaac cggacttatt gtaccaagca tcagaaaagg gcaggggaga ggtatcccta
     3061 agctctactc tgtagaagat tttataatcc taagatggct tgtttcactt cagagaaatg
     3121 ggatttctat acagcgtttt agggatataa ttgaatttct taaagaaaaa attccagatg
     3181 ccattaagaa gcctcagaat tatgtattga ttaccgatgg tacatcaatt caattctttg
     3241 ataaggtgtc tgccaaggca ctagatgtgc taaaagacac aggacaatac ctttttgttt
     3301 tcccagttgg gaaaataatc gagcaaagtg aaaaggcgat caagaaaatc gataaagaat
     3361 aagtttaaag taaattcttt tatgcctctt aagccaaaaa atgtatagaa tttttcctat
     3421 gattcatctt ttctgaacag cacagaagct atatgctcag atcaaagagg taagcttatt
     3481 aagttgtaat aggtttcaat cgcaataagc aagcttcatt agtaggcctt tggttttttc
     3541 aatcggtttt attttatccc tagttctagg tcatagtaat gttctagcca aagtaccaag
     3601 ttggcatttt gatttctatt tctaatcttt agttaacatt ttttatttga ttatagggct
     3661 aggtttgagg tgtctaggaa atgtctattc ccgagttgtt tccatgattt tcattcctat
     3721 tgcatttatt taccaatgat aagggtgcaa caacagggct ctatgtgata aaatataaaa
     3781 agggtttaaa ggtagtttga ttaggggtag gaatttaaaa ttatttccac cccattaggt
     3841 gagggaggaa gtagggaaat tatgacagat tataatcttg attttctatt tattggtgga
     3901 ggcattttgg gtttatcagg ggcgatggcg ctttctaaga ggcttgagga aagaaatcaa
     3961 agcgcacgga tagcagtaat tgatgtggat ttagagggag aacattcttc cacccttaaa
     4021 aatgcaggag gggttcgagc cacatggaaa aaccacgcaa atattgaact atgtaaatac
     4081 tcgattaatt tctacgaaac aattagagaa gaaatccagt ttcgtgaact cggctattat
     4141 tggatgcacg atgaagcatg gtggaaggaa ataaagaaga atcatccact ttataaagaa
     4201 tacggactga ctgttgagct ttatccagga gaagccgtgc ctgaaatttt accctttgta
     4261 gataatctcg atgcaattgc aggcctttct atatcgagaa atgccggctt ggttgaccat
     4321 tattcgctta gggaatatta ccgaagggaa gcaaagaaaa gaggagtcaa gttttttgac
     4381 aggctgtacg ttaagaaaat aatcgtagaa gatgggagag taaccgaaat tattgcacac
     4441 caagtggaaa aaccagaagg aaatattaga gataattccg agttaattaa aacctatctt
     4501 gtgaatggag agattggata tcaaactgag gctgtttcat ttaagtgtga tgtcttaata
     4561 aacaccgcag gggcatgggc tcctaggatt tctaaactct atggctttaa cgatgaaaaa
     4621 atcaaacctc gaagaaggca gatggttgta atacgctgtc ctgaagttga tctgtcacct
     4681 tatggagttg tcatagacac gtcccatgta tattttcata aggacgctga aaacattcta
     4741 gctggatatt caaacatgga tgagccttat ggatacaatc ttgagttcaa ttttggagga
     4801 ttggatgaag gaagtccttt tgtaaagtat atctggcttc ctctttggag gagaatcagt
     4861 aaattcgaga ggataaagtt aataagaggg tgggccggtc tttatgaaga aacccctgat
     4921 cgctcaggct acttagggcg cgttcctggg tttaaaaatg tttatgaatg cggagcgcat
     4981 acaggaagag ggttgatgat ttcctatgga gctgggacgg cgcttacgga tttaatcttg
     5041 gatggcaaat ttagagaaga attaaaacac gcaaaagacc tgtcacgaga aagactctcg
     5101 ggcgagttat tcgaagaact acacttgtag tttttaaacc tctatcttta tcgaatttcc
     5161 ttctaagtga actttataac tttttacaga atttttagct ggtggaccta gaacttctcc
     5221 tgtttttaaa tcgaactctg ctccatgcca aggacagatt acggtatgtt cttggatttc
     5281 tccttctgaa agtggacccc cgacatgggt gcatgtatca tcaatcgcat aataagttcc
     5341 attaatgtta aaaagggcga tattttttcc ttctacttca accatcattc cctgtccagg
     5401 ttctagatcg cctgtttttg caacttctac taggttaccc atcttattct ccgttttaac
     5461 taatttttct agtaagttaa atattaaaat atatactttt ggttgataaa tcaacccata
     5521 ttatcaagga tgtctatggt gtttatgcca aagcccttcc tgatattcaa agacatcttt
     5581 tacctggggt tttatttgcc tgcttaaggg gccattctat ttatgtatct gatgtagcga
     5641 gcaggatgaa aggggtctct ataggggcaa aggagataaa gtttctaaga cttctacatc
     5701 ctcaagctat ccagtctgtc tggctaggtc tctaaacctg cactatgacc ttgcccgccc
     5761 ctagatctgt ctctatcatc tcgcgcaggc tcacatcgcc ttgcgtgaaa atattcgtta
     5821 gcatcccctt catcttcgat gtcttccacc ttgatacaag agtgtggaag gggttggttc
     5881 cgcttagctt ggcaaaatac catgacaccc tcgttccatt ttttattgga gtaagcttag
     5941 tggtcagtcg gaagaatcga ttctttgtga gaactgtgtc caccgtcatg tagtcaaaag
     6001 gtttccagtc aactatagtt tgaattacca tgagttcacc atgtgcacaa tgatattcag
     6061 atccgactcc taatcttcct ttgtcaccac cttgagtagt tatgctgtct gcctcgatcc
     6121 aatgacgtct tagttctggc ttattaagtt aatcccatgc gagggcaggc tgaacgggca
     6181 catccgcctc aacgacgaac caagcctgct tagggtccac gtagacccga cgtcgttcgc
     6241 gttcacgggc ccagaccgag tgaaggtcgt ggatatagcc gctcacatca cccagatgct
     6301 cgtagttccc agtatgcatc ttcatcccct cggtcaattc accaagcgac atggcatcgg
     6361 caaaggcacg agagataaag gcgtaggctt taactccaag tttctcattg attttattct
     6421 taagcaggtt gtgttctaag atgacatccg gtccgctgag ttcctgtcgt cctgctatgt
     6481 cgctcaagac aaaggtgccg tgatgcagaa cgaacttgag gtctagcgta ggaatgagtt
     6541 cgcatgcctt acatctgcag gtagtatcgc gaaacatgct ctccaacgtc atagcaaata
     6601 aacaatagat gttctcaatc gcttcaagca aggtctgccc ttgcacaaaa ctgccctccg
     6661 gcgcgtagac gaaaatggca tctccctcaa gcttagaaac aaaaaagggg gatttcattt
     6721 gttccaatag ggttttgatc aatcttttca ttatcccctc tgcatgatcc agctccgcgt
     6781 gagtcaagaa cgccgtataa ccgctgatat cggcaataac aagataacct ttttgtgaca
     6841 tatattaatc tccttcgata ctcacaattc ttcagcagtc atcagctact tactcaactt
     6901 cgtgtcgtac cctaactcga tgtattcgta ctaaaagaca tagtaaaatt atacgccgat
     6961 taagacatat aatatgcaaa aaacttttct tttccattgt ctcacaaata ataacccctc
     7021 gtcaagaaaa tttctattaa accgtctatt tctataaaaa gaaatggttt tttaccttca
     7081 caggtaaaca aacatatagc aattcctaca ccttttttat aagatgaaag cagtcttcct
     7141 tggccccata aaaatcggag acatttacat ctgagaatta gtctgtgtaa aaacgataaa
     7201 aactgagcct tcagatttta gtatgttaaa ataaatcaaa aatgagtagg aagaagcaaa
     7261 gccagcaaaa aaccggagct aattccaatc tctttaaggt agctaaaatt ggggaggtac
     7321 aatcaggtaa aagaaaagtg gttttagtaa atggcacgga gatagcttta tttaacataa
     7381 acggtgagtt ccacgctata aacaacagat gtccacatgc tgagttttca cttggtgccg
     7441 ggcttattta tgaagatatg gttatctgtc cgggtcacgc ctggacgttt aaattaaaaa
     7501 ctggtgaatg catcactaac aaatctcagc ctgtaaagac ttataaggtg gttatagagg
     7561 gtaaagatat aaagattcag ctttttaacc attttgccac ttagtcacca aggcacaagg
     7621 ggagggttta aggtctaaga gttcaagagt taaacgttaa acctttgaac ttgtaaccct
     7681 taaacctttc ttacctttgt gtctttgtgg ctagttagtt acttaatttt aaattggtac
     7741 ccttctttcg gcattctaat cccattaatg tatatgttat cttctaggtc tattcctact
     7801 cttccttttg ctatgaggtg atggactgca aagggataga tcacccactc tccttctttt
     7861 cttacctttt cctctatttc atttatatct tcttgtttga atccatcaag ggaaatttga
     7921 tctgattgga tgatcgctgg accgaaacgc tcttcttctc tggcaagaaa gatggtgcat
     7981 ttaatcgtat tttctccagc ccttagcgct tgaataggtg cctcgacacc tcgttccaga
     8041 taagtctttg taatatcgcc tggatatatg tttaaaattc tgttcttgta tcgtttgata
     8101 aaaatacttc caagtcgtct tctataacca gctagaacaa ctaggtcggg ataacatatc
     8161 tcttctatga gagtgattgc ggcggcatca tagaaatccc tcgctttatt atcatcaggg
     8221 cctttttgta aaatatcaaa gaacgatttt gaacttagac taatcaccgg aataccgaac
     8281 tctcttgctc tttctactcc tttacagtct ggaaaatttg taaaaaccac ttcaatcctt
     8341 ccatagtttt tctctcctag tctctctaat cttttttgtt cctcatataa ggctaatagg
     8401 tttgtgcctg tgcccgagac aagaactgca acgctcatag gtttattttc tggtttatat
     8461 aatagtttta tttcattcat gctagccagc tatagagcgt ggtccattat tcggctttca
     8521 tgattcgact ccgtcatgag ttcagtcaaa caaacgacta tccacgattc aggaaccccg
     8581 aaatacgcaa ttgatttgct ataatccttt ttttatggaa agtatatccg cttaatgacc
     8641 atgagcaacc tcactcaaag aattcttacc gccgtagtcg gagtacctct cctatatttc
     8701 atcttttatt tgggcgggat tccattcctg gtccttattc tcgccgttat ctttcttgga
     8761 gagatagagt tttttaagct tttggaagta aagggcgttc aatctgaaag ggaattagca
     8821 gtagcattca gtttagcgct agggatagct gcatactttg ggtatttcta ttttgccgtt
     8881 gttttttcat tctcagtaat aatcaccttt ttattagcat taagacaaca ggatttatct
     8941 aacactctca ttgctataag tacaactctt tttgcgatta tttatttagg atggttttta
     9001 agccacgcaa ttttacttag aaacattgat tgtaactcaa accttaaaac ttatgctgaa
     9061 actgttcagg gtcttagcga tgtaggattt ttctatatct tcctagttgt ttcttgtacc
     9121 ttcttaaacg atacaggagc atatttcatt ggaagatgga agggaaaaag aaagcttatc
     9181 ccacatgtaa gtcctggtaa gacagtcgag ggaacgattg gaggcattat ttcttctatt
     9241 attactggcg gggtagcaaa tctcattttt aaagctcctt tggaatacca ctgggttatt
     9301 ttatttggat tagtaatagg aattgttgct gttcttggtg acctaacgga atcattgatt
     9361 aaaaggagcg ctgaagcaaa agactccgga ggtatccttc ctggccacgg cggcatactt
     9421 gatagattcg atagcctaat ttttactttt ccggtttctt actacatggt cctactttac
     9481 tattggttac atggtgtctt agttagctct tagcactaat ttaataagtt ttaacttgat
     9541 aatttccagt agattgctac ggggtctcca caatgctgta ttgtcattgc gaacacgttc
     9601 acttcgttca gtgcaggctc cgtgtagcaa tctttcttcc tgtcattccg agcgaagcga
     9661 ggaatctcgt atttagattg cttcgtcact ttgttcctcg caatgacagt tgcggcgttt
     9721 aatattaaga ccataccctg cagcttggag gctgtccgac aattatggat ggtaaaaatt
     9781 tgacccctca gaatgccaag gatcaacgat cggcaacctg ggaggcgttt tgctaccccc
     9841 aagaatgccg ttttttgcat ttatcgaaca acctccttgc tgcggcgttc ttcattaatc
     9901 ctaaaatcaa ctattttttt ctttttaaat tgagtaaaat tagtagcatc attatgatat
     9961 gaggttaatg agatgggaaa agaatatact aagtttatag ccgctgctgt gcaaacagcg
    10021 cctgtttttt taaacaaaga agagacgata ggtaaggcct gcagtttaat agaagaggct
    10081 agggataatg gagcagattt aatagtattt ccagaggctt ttattcccac ttatccttac
    10141 tggcctaagg acttgggaac aggacccgaa agaaagctta ttttggatgc atatacagag
    10201 ctttataaaa actcagttga aatcccaagc gaagatacag ataagcttcg agaagctgca
    10261 aaaaaggctg gggcttacgt aattattggt atgaacgaaa gagacggtgg cactctatat
    10321 aacacaatac tttttatagg taaaaacggt acgatccttg gcaagcacag gaaacttatg
    10381 tcaatagata gtgaaaagtg tatatgggga atgggtgacg gaagcgacct tagggtgttt
    10441 gatactgagc ttggaagggt aggagggttt ttctgctatg agcatcatat gacattagct
    10501 aaatacgcaa tgtttgctga aggggagcag atacatgcag ggctttgggc agggcatggt
    10561 tttgttaaac ccacaatgga cttcgcaagt agacaatacg cctttgaggg acaggtattt
    10621 gtaatcgcgg cttccctata tattaatgag gatatggttc ccaatagttt tccccttaag
    10681 aaatacacaa aatgggactt cccgggcgga agcggcataa taaatccaag gggggagtac
    10741 ctagctggtc ctctatacga taaagaaggg atcctctatt ccgaaattga tatggatata
    10801 atcataaggg ctaaggctgt aatagatgct atggggcact tttcaagacc tgatatactg
    10861 agcctaaaca taaataaaga aaagtattct ttgataaaaa tcaaaggtag tggagttgaa
    10921 gcttcgtcga atgaacatgg cgagattaag aaagagatga aagagttaag agaaagatac
    10981 gaaagacttg aagctaaaat cgaagagctt attaagggtt caactacagg ttacaaacga
    11041 attaggagat agatagtaac agaagctttt ttgacaaact caatcagtat taacatgtat
    11101 ggttggtgtt ttgctttaat taataatcgt ttggcagaaa tttactttaa tgaaaaagcc
    11161 gtatttggat atacatatgt gtccagtaat gagaaccttg caaagaaaga acaaagatgg
    11221 attcacgctg atattgaaaa atttcgattc acttatagga gacatgtgtt taaaaataaa
    11281 ttcacaggtt aaaaactaaa accagtcaaa tttggtagac aatagaagtt gagacgcaac
    11341 taaacgaaat gctgttttgt ttatttagtt aagaggaatg aaattagttt taatctcttc
    11401 tagggggtaa tgatcttttc tactcctgtc tcccgtccct tctccgtttg aatccctttc
    11461 agggagtaat gagcttttct acccagtccc ctgtcttctg tctcctgtct tctctcttgg
    11521 tttcaattcc tcataggtag gcggctaaac ctgttagcgt gttggcgagt ttgcttgttt
    11581 acgtgttgaa taagtcaagt atgaaataag tttacctatc ttggcagtaa gaagattagt
    11641 ttgggtaact atctcgttaa agtcctcgtc agataaatgg ctaagatttt tagcgatatg
    11701 tacttgtgtt cttacttctg cagttgaacc tttagcataa tacagaaatt taacgaaatc
    11761 cttgttcgtc tttctctcaa aaccctcagc tatatttgac gctattgaaa cagcagcccg
    11821 ttgggcctgg tcttttagcc caaagtcttt agcaaacctt tagctctagc actatagatc
    11881 ataacggcaa gtgttctagc tccctgccaa atttctaggt cttcgaacct ctgtagagtc
    11941 ttcatttagg taacgtttta gagataaata ttattttaaa gagtaacacg gaaatctaat
    12001 tctgttaact tatttacgtg ttaagtaagg tggaatcaat agccataaat ctgtatattg
    12061 gaaaagctgg gtttttaact cacaacccat aaaccagtta acaagttaac cgaactagtt
    12121 tcaattcttg atttaacaat acaacttatg tcataattaa tgttagataa gtaattcata
    12181 atataaaaag aggagggaaa ggctatggca caaaagaaag gtcctaaaga cccacttagc
    12241 gagtttatca gggtttttga acagcagaga aaagaaatgg agaagttagc caaaccattt
    12301 cttaattatc agagtcaaat ggaaaagatg gtaaagccct ttttagatta tcagaaaaga
    12361 agtgagaagg tggctaagcc tattcttgaa taccataaga agctattaga agaaacaaca
    12421 cggtttcagg agacgttcgc tcaaaatatc atagagacaa tagggaagct gataaaccaa
    12481 ataatagagg agcaaaggaa gcaaacggaa gaaacaaata agcttctttc agaagtgcgt
    12541 ctacctaacc aagctacaga gtaccttcac agtatacaga agattcagga aagatggata
    12601 gaacaactaa agaagtctac cgagatgatt gaaagttttg ttaagaccaa ataaaaggga
    12661 caccatacct acctaagaat ttgctccttt agaacatctg tgtactgaag gtgcaggtac
    12721 aggtagttga agagcattcc gtatcaacca acatggcttg gcattcataa ttccctaatg
    12781 ctaaacaatt ttcttcttaa ataaatttct acttgacatt gagtacaatt tttagtataa
    12841 atattaataa gcggctccaa ggggctaagg ctgatttgtt tgaaaaccaa aaactactaa
    12901 gtactgaaag aggagattca aatggtcaaa tcaagtaggg aaaggaaaca atggttttca
    12961 aaaagaacaa ggtcactcgg agtcgttttt atattgtaaa ccaattttcc ttagtaattt
    13021 cttttatatc
//
LOCUS       DATDRL010000004         5843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1306474, whole genome shotgun sequence.
ACCESSION   DATDRL010000004 DATDRL010000000
VERSION     DATDRL010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306474"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            498..1082
                     /locus_tag="VI935_00180"
     CDS             498..1082
                     /locus_tag="VI935_00180"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HLE24044.1"
                     /translation="MGVYYKEKISDKNFNTFMLPSTIPLLGKEGEKQWYAIYTRSRHE
                     KVVYELLNDKGVETFLPLRVLLSQWKDRKKKIESPLFPGYLFTRINILDDFTKVITSK
                     GVVKILGTNGTPIPIPTDEIDSVKTLLKSGLKYDPYPFLKSGMKVQVIRGPLQGIIGK
                     INERLGKYKLLISIELIKRSISVEIDVKDVEPIE"
     gene            1186..2622
                     /locus_tag="VI935_00185"
     CDS             1186..2622
                     /locus_tag="VI935_00185"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HLE24045.1"
                     /translation="MFDNEIEVYTILLAGGVGTRLWPLSRELLPKQFISINGSDSLIQ
                     STIKRLLPIIDVEKLRIVCGESHYHEIKRHIESMSISPIDKIITEPCGRDTAPAILLA
                     TLNILKSEKDAVVLVFPADHMISNVVQFHKKIIAAINLAKIEFIVTLGVRPTYPETGY
                     GYIEGGEAINEGALSIKRFVEKPDQKTAEDYIERGNFFWNSGMFVFKASVIMTEFEAY
                     ESSLLKSMQDMVSIGGPLSNEEYKKLRSISFDYAIMEKTRRGVVIPSEFGWSDIGSWK
                     SLYDFLPKDKDNNVIEGDVISNDTRDCFIKGEGRLIVANCLRNIAVVETADAVLVSDL
                     DKSKNIKSIVNGLAVKGRREYQTHTKVYRPWGYFLVLEEKDNTKVKRIVVNPGARLSL
                     QKHHHRSEHWVIVQGIGKVINGDLTLILEENQSTYIPQNNLHRIENIGKIPLEIVEVE
                     IGDYLEEDDIVRYEDDFGRADSNALEKN"
     gene            2789..4204
                     /locus_tag="VI935_00190"
     CDS             2789..4204
                     /locus_tag="VI935_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HLE24046.1"
                     /translation="MKIIVVGTGYVGLVHAAVCSEYGHEVYAYDNDLEKVDAFSTGKE
                     EDIEKYVIEPGLSIIIRETLGRYLFFSTDFESIAEGSDVIFLCLPTPPNLDGSTDLSI
                     YNTVVELMAQILARRRDKRRVVIVNKSTVPIGTTRHLQKILSQHKVPNFGIASNPEFL
                     AEGTAVDQARRPDRVVVGCDYEEDFKILRRVYSQFVNHVRIKYIETTPETAESIKYVA
                     NTMLLTYISFWNGVGAKIGERISNVKIDDLRIGVTADERISTWGSFVSNGAGGSCFGK
                     DIRSLIYQLRRLDVNTKMLEASYEVNEYQKIYLVERAITEAGFEFNNKTVAVLGLAFK
                     KQTNDMRDASSIRVIESLLGKGVAKINAYDPLAIEEAKSVFSPLKNILFEKIVYCQTP
                     KEALEGSHAVFISSDCDEFRGLSGTIENVVNLPYLIIDGRRMIPDYQELINKGYDYLA
                     VGASFIRGNAKGEERPHLKVL"
     gene            complement(4208..5776)
                     /locus_tag="VI935_00195"
     CDS             complement(4208..5776)
                     /locus_tag="VI935_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HLE24047.1"
                     /translation="MCDLVFDKEDIKQIEARGLTLQKVISQIETFKKGIPFINLLRPC
                     TMSDGITVIEKSDLLRLVEIYSQAALSGRVMKFIPASGASSRMFNLLLSFNNRYEKID
                     VRHISAKADKGDSDYITFFQFIKGIKQLPFYEDLKSAMSKNGLDIEALISNGQYKEIL
                     ENILTSKGLNYANIPKGLIKFHQYTDYSRTPFEEHLIVAALYKLDKNGVTRVHFTVSP
                     VHLDTIKEHIAKFLTRYKNSGVKYEITFSTQKPLTDTIAVDLENKPYRDKGGRLVFRP
                     GGHGALLENLNDIKGDIIFIKNIDNVVPDQIKQDIRIYTKAIGGCLINLQDEIFSYLE
                     RLSRRDVDEQLLKKMAEFARYQLSIIPPEGLVQSSKDERVNFLFSRFNRPLRVCGMVK
                     NDGEQGGAPFWVEHEDNTLSLQIVESSQVNLESDEQRAIWESATHFNPVDLACGVRDY
                     LGKPFNLLDFTDPYTGFISIKSHEGRELKTLEMPGLWNGGMAKWNTVFVEVPKIVFNP
                     VKTIMDLLREQHQL"
BASE COUNT         1877 a          987 c         1178 g         1801 t
ORIGIN      
        1 ctttaagttt ttcgtattct gccttgattc gaccaccttc acaaccagcc attattgaaa
       61 ccgataataa catcactaat gcaacaaaca ttgctgtaac ctttctcata tttactacct
      121 ccttgcaaat tcctttttgg gtaatttaat ctatattcat tacatttaaa tgtcaataag
      181 gaagttattg gaagttattg gagagcaatt aaggaggatt cataataaat caatcgtcga
      241 ggtataggct aagagagcgc cggtgttcct ataagatgtc tttatctaaa tctacggctc
      301 attgttttat ttggttgata ccttagttaa gagatggata atattgacga ttagtattgt
      361 ctgatgcata atatttctag tttttttcaa cttatctatc ttttgataat gaaaaattcc
      421 ggatggagat agggtagggt ggatacgtaa taaaaccttc aaagtatgaa attaagcaga
      481 gatatataag ctttaaaatg ggtgtttatt ataaagagaa aattagtgat aagaacttta
      541 atacctttat gttaccttct acaattcctc tgcttggtaa agaaggggag aaacaatggt
      601 atgccatata tactagaagt aggcatgaaa aggttgttta tgaacttctt aacgataaag
      661 gggtagagac cttcctgcca cttagagtgc ttttgagtca gtggaaagac aggaaaaaga
      721 aaatcgaatc tcctttattc ccaggatatt tatttacacg tattaatata ttggatgact
      781 ttacaaaagt tataacctct aagggggttg taaagatctt aggtacaaat ggaactccta
      841 tcccgatacc aactgatgaa atagattcag taaaaaccct tcttaaaagt ggtttaaagt
      901 atgaccctta tccatttctt aagtctggaa tgaaagtgca ggtaataagg ggccccttac
      961 aaggaataat tggaaaaata aatgaaaggc ttggaaagta taagctgctt atctctattg
     1021 agctaataaa acgatccatc tcagtagaaa ttgatgtgaa ggatgttgaa cctatagaat
     1081 agacttttag agtgtaaagt tttctatgtt caaaaactga attatttttt ataacgcttt
     1141 gtaaggtagt ttgaagatgt ctatagttaa aaggcaagtt tagttatgtt cgataatgaa
     1201 attgaggtct acactattct acttgccggg ggagtgggca cacgcctatg gcccttatca
     1261 agagaactct tgcccaagca attcatcagt attaatggct cggactcgct gattcagagt
     1321 acaattaaga ggcttcttcc aataattgat gtagaaaaat taaggattgt atgtggagaa
     1381 tctcactatc acgagatcaa aaggcatata gaatccatgt ctatttcccc catagataaa
     1441 attattacag agccatgcgg acgggatact gcaccggcca ttcttctagc cactctaaac
     1501 attttgaaat cggaaaagga tgctgttgtt ctagtctttc ccgctgatca catgatctcc
     1561 aacgtagtac aatttcacaa aaaaataata gcagcgatta atcttgcaaa aatcgaattt
     1621 attgtaacat tgggtgtaag acccacatat ccggaaacag gctatggata tatagaaggt
     1681 ggagaagcga taaacgaagg tgctctttct attaaaaggt ttgtggaaaa accggatcaa
     1741 aaaacagcag aggattatat agaaagagga aacttttttt ggaatagtgg tatgtttgtt
     1801 ttcaaggctt ctgttattat gacagaattt gaggcttatg agtcctctct tctaaaaagt
     1861 atgcaggata tggtttcaat tggcggcccc ctttcaaatg aagaatataa aaagctacga
     1921 tctatttcat ttgattacgc tataatggag aaaacgagaa ggggtgttgt tatcccttct
     1981 gaattcggat ggagcgatat tggttcttgg aaatcccttt atgattttct tccaaaggac
     2041 aaggataata atgtgataga aggagatgtc atttcaaacg atacaaggga ctgttttatc
     2101 aagggtgaag gaagactaat agtcgcaaat tgtcttagaa atatagcagt ggtagaaacg
     2161 gccgacgcag ttttagtttc tgacttggac aaaagtaaaa atattaagtc tatagttaat
     2221 ggcctggcag ttaaaggacg cagggaatac caaacacaca cgaaggttta ccgtccttgg
     2281 ggttactttt tagtgttaga agaaaaggat aatacaaaag ttaaaagaat cgtcgtcaac
     2341 ccaggtgcaa gattgtctct tcaaaagcat caccatagaa gtgaacactg ggttatagtt
     2401 caaggtattg ggaaagttat taatggtgat ctcacattaa ttttggaaga gaaccagtcc
     2461 acatatattc cccaaaacaa tcttcatcgc attgagaata taggcaaaat tccacttgaa
     2521 atagttgagg tagaaattgg agattaccta gaagaagatg atatcgttcg ctatgaggat
     2581 gattttggaa gggcggattc taatgcttta gagaaaaatt aaaatgatta tgctttacta
     2641 agggattctg ataggatcaa cgagcttgtc tttaatatta aaaataggcg attggaaaat
     2701 aaacattgta atggtatctt gataaacttg aattaaaaaa taggtggata ttcaggaaac
     2761 agcagtcatg gactaaaagg aggcctagat gaaaatcata gttgtaggaa cggggtatgt
     2821 aggattagta cacgcagcag tgtgttctga atatggtcat gaagtatatg catacgacaa
     2881 cgaccttgag aaggttgatg cgttttcaac tggaaaagaa gaggatattg aaaaatatgt
     2941 tattgaacca ggtcttagca taattataag ggagacgctg ggtaggtact tattcttctc
     3001 tacggatttt gaatcaatag cggagggctc ggatgtaatt ttcttatgtt tacctacacc
     3061 acctaatctt gacggttcaa ctgatctaag tatctataac actgtcgttg agctaatggc
     3121 tcagattcta gcaagacgaa gagacaagcg tagagttgtt atcgtgaata aaagcacagt
     3181 tcctattggt actacaagac acctgcagaa gattctatct caacataagg ttccaaactt
     3241 cggtatcgcc tcaaaccctg aattccttgc agaaggcaca gcagtggatc aggcacgaag
     3301 acctgatcgg gttgtggtag gttgtgacta tgaagaggac tttaaaatcc tacgtcgtgt
     3361 gtattcccaa tttgtaaatc atgtcagaat aaaatatata gagacaaccc cagagacagc
     3421 cgagtctata aaatacgttg ctaacactat gctcttaaca tatatatcat tttggaacgg
     3481 tgtaggagca aagatcggag aaaggatctc caatgtgaaa attgatgatt tgagaattgg
     3541 agtaacagcg gatgaaagaa taagtacctg ggggtcgttt gtgagcaacg gggctggtgg
     3601 aagttgtttt ggaaaggata ttcgttcact tatttatcaa ttgagacgcc ttgatgtaaa
     3661 tactaagatg ctcgaagcct cttatgaagt gaacgaatat caaaaaatct atctggttga
     3721 aagagctatt accgaagctg gcttcgagtt taataacaaa acggttgctg tgctagggct
     3781 ggcgtttaaa aagcagacca atgacatgcg cgatgcctct tcaattaggg tcatagaatc
     3841 cctgcttgga aagggggtag ccaagataaa tgcatatgat ccattggcta tcgaagaagc
     3901 taaatctgtt tttagtccat tgaagaacat cctttttgaa aagatagttt actgtcagac
     3961 cccgaaagag gctctcgaag gctctcatgc ggtatttata tcatccgatt gtgatgaatt
     4021 tcgtggactt tcaggtacta tagaaaatgt tgttaactta ccttatctga taatagacgg
     4081 aaggcgcatg atccctgatt atcaagagct gattaacaaa ggatacgatt atcttgcggt
     4141 gggagcatcc tttatcaggg gaaatgcgaa gggcgaggag aggcctcatc tcaaggtgtt
     4201 ataaatatta aagttggtgt tgttctcgga gcaagtccat tattgttttt acaggattaa
     4261 atacaatttt aggaacttca acgaaaacag tattccactt agccatgccg ccgttccaca
     4321 gtccgggcat ctcaagtgtc ttaagttctc tgccttcgtg agatttgatt gatatgaacc
     4381 ctgtataagg atccgtaaaa tccaaaagat taaatggctt acctaaataa tcacgaacac
     4441 cacaagctaa atcaactgga ttaaaatgag tagctgattc ccatatagct cgttgctcgt
     4501 ctgattctaa attaacctga gatgactcta caatttgtaa tgataaagta ttatcttcat
     4561 gctctaccca aaaaggtgcg cccccttgtt caccatcatt cttaaccatt ccgcataccc
     4621 taaggggtct gttgaatctt gaaaaaagaa aatttactct ttcatctttt gagctttgca
     4681 ccaagccttc gggtggaatg atagagagct gataccgagc aaattcagcc atcttcttta
     4741 gtagttgctc atcaacatct ctcctagata acctttctaa gtaactgaat atctcgtctt
     4801 gcagatttat taaacaccct ccaattgcct tagtataaat tctaatatct tgctttattt
     4861 gatcaggcac cacgttatct atattcttta tgaatataat gtctcctttg atgtcattaa
     4921 gattttctaa taaggctcca tgcccccctg gtcgaaatac aagtcttcct cccttatctc
     4981 gataaggttt attttctaaa tctacggcga tagtatctgt taaaggcttt tgagtggaaa
     5041 aggttatttc atatttcact cctgaatttt tgtagcgagt taagaacttc gctatatgtt
     5101 cttttatagt gtctaggtga acaggagata ctgtaaaatg cacccgagta actccgtttt
     5161 tatccagctt gtaaagcgct gcaacaataa gatgttcttc aaagggggtg cgagaatagt
     5221 cggtatactg atgaaacttt ataagtccct tggggatatt agcgtagtta agcccttttg
     5281 atgtcaatat attttctaga atttccttat actgaccatt tgatataagg gcttcgatat
     5341 ctaaaccgtt ttttgacatt gctgatttca aatcttcata aaatggtaac tgctttattc
     5401 cctttataaa ttggaaaaag gttatataat ctgaatcacc cttgtcagcc tttgcagaaa
     5461 tatgtcttac atcaatcttt tcataacgat tattgaaaga aagcaatagg ttaaacattc
     5521 gacttgaagc gcctgatgcg gggataaatt tcattactct tccggataag gcggcctgtg
     5581 aataaatctc cactagtcta aggaggtcac ttttctcaat tacagtgata ccatcactca
     5641 tagtgcaagg cctaagcaag tttataaagg gtatcccttt tttgaaagtc tctatttgag
     5701 aaatgacctt ttggagcgta agtcctctag cctcaatttg cttaatatct tctttatcaa
     5761 aaactaaatc gcacatcttc taattatcct tactatttat atattataca attcaatcaa
     5821 attgtggtgt aacaaaaagt cgt
//
LOCUS       DATDRL010000005         2594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3944705, whole genome shotgun sequence.
ACCESSION   DATDRL010000005 DATDRL010000000
VERSION     DATDRL010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3944705"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VI935_00200"
     CDS             complement(<1..413)
                     /locus_tag="VI935_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLE24048.1"
                     /translation="MIEEAYIKLRPKSKVLYEKAINIFPSGVTHDTRFILPFPTYITE
                     ARGSRKWDVDGNEYVDFAMGHGALLLGHGREEVINAVREAVLKGTHYGGSSELEIKWG
                     EIVKRLVPSAERVKFTSSGTEATLLALRIARAYTG"
     gene            831..1445
                     /locus_tag="VI935_00205"
     CDS             831..1445
                     /locus_tag="VI935_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24049.1"
                     /translation="MKRIFYLIPLIFILGCAKSLPQKEARPIKPKETPTLKQYYVFNA
                     PFSEVWSATLQGLKWMKWNPAFVEKEEGTIRLKEAYVYTKSGRLFRNYRWPSKEEAKQ
                     SDIDDYVDKVVVYDKNIFDFDRPVFSQENMTIEVIKLSKSQTKVNINYKINIYLDSGK
                     FASGVRSSVYIESLLFEQIKEGLNGKPLSYDNKIYEENSILSLQ"
     gene            1620..2147
                     /locus_tag="VI935_00210"
     CDS             1620..2147
                     /locus_tag="VI935_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24050.1"
                     /translation="MNSILILSAFIIAIILLFLLDRKSKTEKPEVERIYNEIFKIFNK
                     IKWGMAMGELREAFKDKEFVTSEESGEVMGTGFMDKLDGQDIFISFYFPKGGKDTLIR
                     ADYYLIGMPTSKVNPLFSKFIEKYGTPLPQNSSDQKSSSWDLGNSLLTLEPTDGEALQ
                     IQLWSKEFYNKIDKM"
     gene            complement(2240..>2594)
                     /locus_tag="VI935_00215"
     CDS             complement(2240..>2594)
                     /locus_tag="VI935_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HLE24051.1"
                     /translation="TSVFTCSRFGRIVICAGTSGYDCVFDVRHLWMHQKRIIGSHFAN
                     ALECLRANELIRKGIIKPVLSHTYSYDEIPKVHQIMYENKHSGKMAVLVQAGQLGLKN
                     TEEARSAAKSLQARA"
BASE COUNT          821 a          530 c          512 g          731 t
ORIGIN      
        1 tttccggtgt aggcgcgcgc gattcttaga gcaagaagcg ttgcttcagt gccagagctt
       61 gtaaacttca ctcgttccgc cgatggaaca agccttttta ctatctcacc ccattttatt
      121 tcaagctcac ttgagccccc ataatgcgtt cctttaagaa cggcttccct cacagcatta
      181 attacctcct ctctcccatg ccctaggaga agagcgccgt gtcccattgc aaagtccaca
      241 tattcgttcc catcaacatc ccatttccta gagccccttg cctcagttat ataagttgga
      301 aatgggagga taaaccttgt gtcgtgtgtt acaccacttg gaaagatgtt tatagctttt
      361 tcatatagga cctttgattt gggtcttagt tttatgtacg cctcttcaat cataaaagtg
      421 tctccttaac cctggtgacc atttaaccac tattaaacac ataaaatgat ccatgatgca
      481 ggattcatgt tacacgaaag tttttaaatg catcctgcac ccctctaccg agctcacgac
      541 gaagctacta cctgcgtttt cgtggctaaa tagttacgtt aagtttacac caaaacagta
      601 agttaattag gagttgtttt tttattttaa tgctcagatt ttttggggta ggttacgagt
      661 cccctcaacc aaactcggga caggcttgct cgtaacctcg tgatgattca atctccacca
      721 gcaagcctgt cctgagcgaa gtcgaaagaa gtggtggacc cactggttga cggactagat
      781 agttgaaact agaaagcatt gaagggataa taagataaag gacaaacttg atgaaaagga
      841 ttttttatct aattcctctg attttcattc taggttgtgc taagtcattg ccgcagaaag
      901 aggcacgacc aattaagcca aaagagacac ccacccttaa acaatactat gtatttaatg
      961 ctccattcag cgaagtctgg tctgcgaccc tgcaagggct aaaatggatg aagtggaatc
     1021 cagcctttgt agaaaaagaa gaaggaacta ttagactaaa agaagcctac gtttatacaa
     1081 aatcaggaag gctatttaga aactatcgct ggccctctaa agaggaggct aaacaatcgg
     1141 atatagatga ttacgttgac aaagtggtcg tctatgacaa aaacatattt gattttgata
     1201 gaccggtttt ttctcaagaa aacatgacaa tagaagtaat taagctctca aaatcacaaa
     1261 ctaaggttaa catcaactat aaaatcaata tttacttaga ctccggtaaa tttgcaagtg
     1321 gagttagatc aagtgtatac atagaatcgt tgcttttcga gcaaattaaa gagggattaa
     1381 atggtaaacc tttgtcgtat gataacaaaa tttatgagga aaattctatt ctttcattgc
     1441 aatgacattg tggactaaag gtgtcattgc gaggagccct tcaatttggc tcagatgaac
     1501 tccgcggcga agcaatccca aaaagagaga ttgcttcgga acttcgtccc tcgcaatgac
     1561 agaaaaagcc cagcagcaac ctacaggaaa ttaacaagtt aaagaggaag gataaacaaa
     1621 tgaacagcat tttaatctta tctgcgttta ttatagctat aatactcctc tttttgttag
     1681 ataggaaaag caagacagaa aaaccagaag ttgaaagaat ctacaatgag atatttaaga
     1741 tctttaataa gattaaatgg ggtatggcaa tgggtgaact aagagaagca ttcaaggata
     1801 aggagtttgt gacttcagaa gaatctggag aggtgatggg cacgggcttt atggataaat
     1861 tagatggaca ggatattttc attagcttct attttcccaa aggaggcaaa gacacactaa
     1921 ttagagctga ttattacctt attggtatgc ccacatcaaa agttaatcca ttattcagca
     1981 agttcataga gaaatatgga acccctcttc cgcaaaatag cagtgatcaa aaatccagtt
     2041 catgggatct aggaaatagc cttcttactc tagaaccaac cgatggcgaa gcattacaga
     2101 tacagctatg gagtaaggaa ttttacaata aaattgataa aatgtagggg caggtcttgt
     2161 atctgcccta ttcttttgga caaccgcaag ggttgcccta caacaataac aattatgaaa
     2221 ggttatgcgt tagcagcaac taggcgcggg cctgaaggga tttagccgca ctccttgctt
     2281 cctccgtatt cttcaacccg agttgacccg cctgcacaag aactgccatt ttgccagagt
     2341 gcttattttc atacatgatc tgatgcacct tcggaatctc atcataagaa tatgtatgag
     2401 atagcacagg ctttattatt ccctttctta tcagctcatt tgcacgaagg cactcaagtg
     2461 cattagcaaa gtgtgatcct attatccgtt tttggtgcat ccataggtgt cttacatcaa
     2521 aaacacagtc atatcccgat gttcctgcac aaataacaat tcgaccaaat cggctacatg
     2581 taaatacact cgtt
//
LOCUS       DATDRL010000006         8348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2099992, whole genome shotgun sequence.
ACCESSION   DATDRL010000006 DATDRL010000000
VERSION     DATDRL010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2099992"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VI935_00220"
     CDS             complement(<1..327)
                     /locus_tag="VI935_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24052.1"
                     /translation="MKRYLLLAMLLTLAAYAVPTYAADPTEGSVSVLFVQTSKGVAFD
                     KGTMTLKGVSPSTVFFSDRPNRIAGHLPTSHFLKVWDEGKDNFKNDPPNANVTILGKT
                     EGATNVV"
     gene            709..1794
                     /locus_tag="VI935_00225"
     CDS             709..1794
                     /locus_tag="VI935_00225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLE24053.1"
                     /translation="MDYGYFTMPLHPQGSNMTETLDEDLEQIVILDNLGFKEAWIGEH
                     FTSSWENIPAPDLLIAKAIPLTKNIILGIGVSCMPNHNPFVLAHRIAMLDHLAHGRFY
                     WGVGAGSFPGDFKVFGINPKTGEQRVLTHDAIDLILKLWEDPQPGLYENKYWRFSVPE
                     PQDDVGLRLHLKPYQKPHPPIAVAGISEGSGTLTIAGERDWIPMSINLVAKQIIRSHW
                     KAVEEGAQKGGKIADRSKWRVARDVYVAETTEQARREAIEGTLGRDFKEYFFRLVPKI
                     RGSLDIFKTDTGISDSDVTLDYMVDNMWIVGSPDDVAHKIRELYEYLGGFGVLLVMGH
                     EWRPRDKWVRSMALLVKEVMPKLQDLG"
     gene            1888..3537
                     /locus_tag="VI935_00230"
     CDS             1888..3537
                     /locus_tag="VI935_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLE24054.1"
                     /translation="MGRSLLFNKLARTIRIAHHCEENDISTKEGLERVADLEARLRKR
                     RASRRDFLGAMGKVAVVGAIGSVAWPLRSTLAAPPPGPVVNIAIVGAGLAGITCGGEL
                     RKNGINATLYEANNNRVGGRCFSLRGFFPNDQVAERGGEFIDNLHKTMLGYAQEFNLQ
                     LEDVEKEPPGEVFYFFNGQHFPESQVVDEFRNLVAAMHDDLRVLTPPTADNHTEADED
                     LDRTNLQEYLDTRGAGDLIKSAVKAAYIAEYGLEIEEQSCLNFLLFIHLDKRSKFRPF
                     GVFSDERYHIIGGNDQIVEGLRSKFQDQIELGMLLVRARKTSTGRVELTFKDGARTVT
                     VTHDAVVFAIPFSTLREVELDESLELTTEKRLAINELRYGTNAKMMIGFNSRPWIAQG
                     SKGTSYSDLLNHQNTWETNPIKATNTHAVLTDYSGGHRGASLNPQKVQNEAKLFLTDL
                     DRVYPGAFAAAFRDTKGRFRAHLEHWPSNPLVKGSYTCNHPGYFTTIADNEAKKVGNL
                     YFAGEHTSSFYEWQGFMEGAALSGIRAAQEILQDIKDGDLG"
     gene            complement(3836..4672)
                     /locus_tag="VI935_00235"
     CDS             complement(3836..4672)
                     /locus_tag="VI935_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLE24055.1"
                     /translation="MTLTKRISYLWLMLCVSTALAVELNVSNLADLLIKAQKENQLIP
                     VLSNQYPELHVETAYLVQRAYVKRKLSDDQIAGFKAGLTSSEIQKKFCVTFPVSGVLF
                     GSGKKLNSPMIDKSIFKVPMIETEIGFLVGKPITHSLKNVSDLYEAIQAVLPVVELPD
                     LGFTDLKKVKVADIIAANVSSSQFIVGTEKHTKSLDLDNIIVTLSLNGQVVNQGKGRD
                     ALGDQWKAALWLVNAITKQGRKIEPGYILITGVLGKMIPRKEGKYIADYGELGKIEFE
                     IK"
     gene            4881..5312
                     /locus_tag="VI935_00240"
     CDS             4881..5312
                     /locus_tag="VI935_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLE24056.1"
                     /translation="MPNNSDYEEVLKVNEMFYKALGTRNLELMDQVWVKDSRAGCVHP
                     GWIILSGWQAIRQSWENVFDPNDQVDIRLSNITVEIKRDVAWVTCIQQLIYINRDPIG
                     INMSQSTNIFERHDSGWLMILHHASPIPITNYEIADRSNLQ"
     gene            complement(5798..6130)
                     /locus_tag="VI935_00245"
     CDS             complement(5798..6130)
                     /locus_tag="VI935_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24057.1"
                     /translation="MIRVQIVEQHGTNLYKTLVEAMRSGKLRTFSVTKRGRRVTHTKS
                     DYPGWMNWSYADGIIVCEVLSPRKPGSEWKLLSALIGRLADKYADCIHSISIQFPDAI
                     VPESTKRR"
     gene            complement(6179..7858)
                     /locus_tag="VI935_00250"
     CDS             complement(6179..7858)
                     /locus_tag="VI935_00250"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLE24058.1"
                     /translation="MIWKLKEKAKDILSNETGTIFKEGSLRVALAYPNTYQIGTSNLG
                     FQLIYRILNSIPNISAERVYLPDKEDIKHYESGEDLFSLESQRQIGEFDVIAFSIPFE
                     GDYINVLKILELSKIPLRGIDREENYPLIIAGGMATLLNPEPLAPFIDAFVIGEGEYI
                     TREVFEILARRELKWKTLERLSKVKGVYVPSFFEPNYNEDGTFAGHLPSLSVQKRFVD
                     DLHLHPAGSTILTKNTTFSDTYLIEVSRGCGGACRFCAEGFVYRYPRHRDKESIITEI
                     KKAKELGRRVGLISPMLLDHPDLKEIIAEAKKEGIQASLSSLRADALDEDMLETIRMS
                     GQKTLTIAPEAGSDFLRRSVNKHFTNGDIIRSAELSFKFGITNLKLYFMVGLPGETDE
                     DVEAIGFLGKAIRDVFPRGKVTIGVNPLVPKPWTPFQWAPFPRLEDVERKYRLIKDEV
                     KEISGIELSLSSPRLFYIQSLLSRGSRRVSRFLELALEEKGSYARALRKWEFNPDFFT
                     YREHRADETFPWDFIDHGISKRFLRKEYEKSFEGRKTPECVVPICKLCGVC"
BASE COUNT         2317 a         1741 c         1765 g         2525 t
ORIGIN      
        1 tacaacgttg gtcgctcctt ctgttttacc aagaatcgtt acattagcgt ttggagggtc
       61 gtttttaaag ttatccttac cttcatccca aacctttagg aaatgcgagg taggcaaatg
      121 accagctatt ctgtttggac ggtctgaaaa gaaaactgta gacggactta cgcctttcaa
      181 tgtcattgtt cctttatcaa aggctacgcc ttttgaagtt tgaacaaata atacactaac
      241 cgaaccctca gttgggtcgg cggcataagt aggcacagca tatgccgcta atgttagcaa
      301 cattgcaagt aaaagatacc tcttcataat ttcttcctcc ttacaacaaa tttttgattt
      361 gaatttagcc tccggcaaat taatatttag gaggctgctt caaattccca tttcatatat
      421 cattttaaaa cttaggtgtc aagattatta ttatgcctgg aaacgactta aaagtaaggg
      481 attattttct ttgatctaga tatcacaata aaataaaaaa cgagaatttc ttgaatcaat
      541 cggctaataa aaatcttttg tttgtattac ttgttccagt ataattattt tccgattcga
      601 tcaaatagtg aatgagctaa agttctgagt cgaggtgtgg ttgtgtgcta tcgaaggttg
      661 gaatatgtag agctatacga tttctagtga tgtgaaacgg aggttcagat ggattacggt
      721 tactttacca tgccacttca tccacaggga tcaaacatga cagagaccct tgacgaggac
      781 cttgaacaga ttgtgattct agacaatctt ggatttaaag aggcttggat cggagagcac
      841 ttcacctctt cttgggaaaa cattccagct cctgacctcc tcatagcaaa agccatcccc
      901 ttaacaaaga atatcatact tggcattggt gtctcctgta tgccaaacca caatccattt
      961 gtactcgccc acaggatcgc aatgttggat cacctggcac acggccgctt ttattgggga
     1021 gttggggctg gtagctttcc aggagacttt aaggtctttg gaattaatcc taaaacaggc
     1081 gaacagcggg tcttaaccca tgatgctatt gatctgatac tcaagctatg ggaggatccg
     1141 cagcccggac tttacgaaaa caaatattgg cgattttcgg ttcctgaacc tcaagatgat
     1201 gtgggactaa ggttacatct gaagccttat caaaaacccc atcctccaat agctgtcgct
     1261 ggtatttctg agggatctgg aacattgacg attgctggtg agcgcgattg gattccgatg
     1321 agtattaatc ttgtagctaa gcaaatcatt agaagtcact ggaaggcagt tgaagaaggc
     1381 gctcaaaagg ggggaaagat tgcggatcgc tcgaaatgga gggtagcgag ggatgtttac
     1441 gttgcagaga cgactgaaca agcgcggcgt gaggcaattg aaggaacctt aggcagggat
     1501 ttcaaggaat actttttccg actcgttcca aaaatcaggg gttctttaga catttttaaa
     1561 actgatacag gtatatccga ttcagatgtt acccttgatt acatggttga caatatgtgg
     1621 atagttggta gtccagacga tgtagctcat aagattcgag aattgtatga atacctcgga
     1681 gggtttggcg ttcttcttgt aatggggcat gaatggcggc caagagataa gtgggtgcgc
     1741 tcaatggcac tcctggtaaa agaagttatg ccaaaacttc aagacttagg ctaggatttt
     1801 attctcttta tgttagcgat tatatttatt tctaattcgt tgttgagttt tacttaggtc
     1861 ataggatctt tttaaggagg aagccatatg ggtcgttcgc ttcttttcaa caaacttgcc
     1921 cgaacaatac ggattgcgca ccactgcgaa gaaaacgata tctctactaa agagggttta
     1981 gagcgggttg cggatttaga ggcacgcttg cggaagcgcc gtgctagcag gcgcgatttt
     2041 cttggtgcca tgggtaaggt tgcggtagtc ggcgctattg gttcggttgc gtggccttta
     2101 cgaagcacat tagcagctcc ccctccaggt cctgttgtta atattgccat cgtaggtgct
     2161 ggcctagcag gaattacttg cggaggtgaa ctaaggaaga acggtattaa tgcaacatta
     2221 tatgaagcaa acaataatag ggttggcggt cgttgcttct cactccgagg ctttttccct
     2281 aacgatcagg ttgcggagcg gggaggcgaa ttcatagaca acctgcataa gacaatgctt
     2341 ggttacgcac aggaattcaa tcttcagctt gaggatgtag agaaagagcc tccaggtgag
     2401 gtcttttact tttttaacgg acagcatttt ccagagtctc aagttgttga cgagttccgt
     2461 aatctggttg cagcaatgca tgatgacctg cgcgtgctaa ctcccccgac cgccgataat
     2521 cacactgagg ccgatgagga tctcgaccgt acgaacctac aggagtacct cgatacccgc
     2581 ggggcgggag acttgatcaa gtccgcagtc aaagcagcct acattgctga gtacggtctt
     2641 gagatcgagg agcaaagctg ccttaatttt cttctcttta ttcacttgga taagcgatca
     2701 aaatttcgcc ccttcggtgt ttttagtgac gagcggtatc acattattgg gggtaatgat
     2761 cagattgttg aaggacttcg ctctaagttc caggatcaga tcgagcttgg aatgctgctt
     2821 gtgagagcgc gaaagacctc tacaggaaga gtcgagctta cattcaaaga tggtgctaga
     2881 actgttaccg taacccatga cgctgttgtc tttgctatac ctttttcaac tctacgcgag
     2941 gtcgagttgg atgaaagcct tgagcttacc accgagaagc gtcttgcaat caacgaactt
     3001 cgctacggca caaacgcaaa aatgatgatc ggatttaata gccgtccctg gatagctcaa
     3061 ggaagcaagg gtacgtctta ctctgacctt cttaaccacc agaatacctg ggagacaaat
     3121 cctataaagg caacaaatac acatgcggta cttacggact actcaggcgg gcaccgaggt
     3181 gcaagcttaa atccccagaa agtccagaat gaggcgaaac tttttctaac agaccttgat
     3241 cgcgtctacc caggagcatt tgcggcagcc ttccgcgata cgaaaggtag attccgtgca
     3301 caccttgagc actggccttc aaaccctctc gtgaaaggaa gttatacgtg caaccatcca
     3361 ggatatttta cgacgatagc tgacaacgaa gctaagaagg tcggaaacct ctacttcgct
     3421 ggtgagcaca caagctcctt ctacgagtgg cagggtttca tggaaggtgc tgccctttca
     3481 ggcattcgag cagcacaaga aattctccag gatattaagg acggtgattt aggctgattc
     3541 taagtgataa gcttactctc tggtttatta atctaattta actcgacaat tccccctagc
     3601 ttgctatagg atttctactg tcattacgag cgttgcggtt ccctaactac cctccctcca
     3661 ttattgctac cacagatccc agcgaagcga tctcgtcttt cttgagattg ctccgtcact
     3721 tcgttcctcg caatgaccta atggaatgtc tgtttatatg ccgataccgt gcaccttgct
     3781 tcagggcatt tcattttagg aaatgtggta agcattaatt tcagattaga taggatcact
     3841 taatttcaaa ctcgatcttc cccagttctc catagtctgc tatatatttc ccttcctttc
     3901 taggaatcat ctttcccaga actccagtga tgaggatata gcctggttca atcttcctac
     3961 cttgctttgt tatggcattt acaagccata gtgcggcctt ccactgatca cccagggcat
     4021 ctcttccctt accctggttg actacctgtc cgtttagtga aagtgttact attatattgt
     4081 caagatctag actctttgta tgcttttcag ttccaacgat gaattgagat gatgagacat
     4141 ttgcagctat gatgtcggct actttaacct ttttcaagtc tgtaaaccct agatctggaa
     4201 gttcaacgac aggcagtact gcttgaatgg cttcataaag gtcggatacg ttcttaagtg
     4261 aatgtgttat aggttttccc actaggaatc ctatttctgt ttcaatcatc ggtaccttaa
     4321 atatagattt gtcaatcatc ggtgagttaa gcttctttcc agacccaaag agaacacccg
     4381 atacaggaaa cgtcacgcaa aacttttttt ggatttctga agaagtaagg ccagccttaa
     4441 accctgcaat ctgatcatca gacagctttc ttttgacgta ggctctctgc actaggtaag
     4501 cagtctcaac atgaagctca gggtattgat tagaaagaac ggggattagc tggttttcct
     4561 tttgggcttt tattaagaga tcggctaagt tggaaacatt taattcaacg gctaatgcgg
     4621 tagatacaca tagcataagc cataaatatg aaatcctttt tgttaatgtc atctccactt
     4681 cttttttgat ttacaatttg tttcgctttt gagaaatctt aatatggaaa aagtaaatat
     4741 acatattgat tcctgaatca tagcgcttgt ttatctgtca atctatagat aaaataaagt
     4801 taagaagatt tgaggttttt gaaatcaatt tcacttgata aatcttaaac aaaccaatct
     4861 tataaaaaca ggggattctc atgcctaata attcagacta cgaagaggtg ctgaaggtaa
     4921 acgaaatgtt ttataaggcg cttggtacaa gaaacctgga actaatggat caagtctggg
     4981 tcaaagactc aagggcaggg tgtgtacacc caggctggat aatacttagt ggatggcagg
     5041 ctataagaca gagttgggaa aatgtattcg acccaaacga tcaagtggat atcaggcttt
     5101 caaacattac tgtggaaata aaaagggacg ttgcatgggt tacttgtata cagcaactaa
     5161 tttacataaa tagagacccg atagggataa acatgtctca gtcaacaaac atttttgaac
     5221 gtcatgattc cggctggctc atgattctac atcatgcttc tcctataccg ataacgaatt
     5281 atgaaatcgc agatagaagt aatttgcagt agttcttttt gttctttctt atacggattg
     5341 ttttttttct ctaaatcttg tctgaaaagt tttcgtcgga gggatcaaac ggagggacgg
     5401 ctataacgca gagtctattt ttctccgatg attctatgat ctcatgatgt gggcaacgcc
     5461 ggttttaaga tccaacattt gctctacagt taaccgcgca tccattcagg caacaattgc
     5521 cagcttacga gcgggtggta taggaagata tcccagttta aagttctttg catagtcttt
     5581 gttagccttt agaacctcat caattaaact catgtattta ggcctcctaa ctatttttac
     5641 ttaaaattag caagattaat gccagaggta agtatcttta attacaacaa attataatat
     5701 gaatggaaga attagctggt tactttttaa ccaaatagta ttgctctgtt acaaggatga
     5761 aaaaactata caagcatagc agcttcgcct gaacctatca gcgacgcttg gtggattcag
     5821 ggacgatcgc atccgggaac tgaatgctta tgctgtggat gcagtcggca tatttgtctg
     5881 ctaaacgtcc gataagggcg cttaacagct tccactcgct ccctggctta cgtgggctta
     5941 gtacttcaca aacaattatt ccatccgcat aagaccagtt catccaacca ggataatctg
     6001 atttagtatg agtgaccctc cgcccacgtt tcgttacaga gaaggttcga agctttccag
     6061 agcgcatcgc ctcgactaga gttttataga gattggttcc atgttgctca acgatctgaa
     6121 ccctgatcat aatcctctct ccatttgtgc tgtcatctaa tttaaattgt acattttatt
     6181 agcacactcc gcagagtttg catataggca ctacgcattc aggggttttt cttccttcga
     6241 atgatttttc gtattccttt cttaaaaacc tttttgatat tccatggtca ataaaatccc
     6301 atggaaatgt ttcgtccgcc cggtgttcac gataggtaaa gaagtcaggg ttaaattccc
     6361 attttcttaa cgctctagcg tagctccctt tttcttcgag tgctagttca aggaaacgag
     6421 aaacacggcg tgagccgcgt gatagaaggg actggatgta gaaaagtctt ggggagctca
     6481 aagaaagttc tatcccgctt atttctttaa cctcatcttt tataagtcgg tattttcttt
     6541 cgacatcttc aagcctagga aatggagccc actgaaaggg agtccaaggt tttggtacga
     6601 gtggattaac accaatagtc actttgcctc gtggaaaaac gtctcttatt gcttttccga
     6661 gaaacccaat tgcttctaca tcttcatctg tctctccagg aagtcctacc ataaagtaga
     6721 gtttcaagtt tgttattcca aacttaaacg aaagctcggc acttctaatt atatccccgt
     6781 tcgtaaaatg tttatttaca gagcgtctta gaaaatcgct accagcttca ggcgcgattg
     6841 taagggtttt ctgtccactc atacgaatag tttcgagcat atcttcatca agagcgtctg
     6901 ctctaagtga agaaagagag gcctgtatac cctccttttt agcctcggcg attatttcct
     6961 ttaaatctgg gtggtcaagg agcatagggc ttatcagtcc tactctcctt cccaattctt
     7021 tggccttctt tatttcagtg ataattgatt ctttatctct gtgcctcggg tatctgtata
     7081 caaacccttc tgcacagaac cggcatgcac ctccgcaccc tctacttact tcgatgagat
     7141 acgtatcaga aaaggttgtg ttttttgtaa gaatcgtaga tccagcagga tgtaaatgga
     7201 ggtcatctac gaatcttttt tgtacactga gagagggaag atgccctgca aatgttccat
     7261 cttcgttgta attaggctca aagaaagaag ggacataaac acctttaacc tttgagagcc
     7321 gctctagagt cttccacttt agttctctgc gtgctagaat ctcgaaaact tctctagtta
     7381 tatattcgcc ttcgcctatc acaaatgcat caataaaagg agcaagcggt tctgggttga
     7441 gaagggttgc catcccccct gctattataa gcgggtagtt ttcttctcta tctatgcccc
     7501 gaagagggat ttttgataac tctaggattt tgagcacatt tatataatcg ccctcgaacg
     7561 ggatagagaa ggcgattaca tcaaactctc caatctgcct ttggctttcg agtgaaaaaa
     7621 ggtcctcgcc cgattcatag tgctttatgt cttccttatc ggggagataa accctctctg
     7681 ctgagatgtt agggattgaa tttaggatgc gatatattag ctgaaaccct aggtttgacg
     7741 ttccaatctg gtatgtattt ggatatgcga gagcaactct tagcgaacct tctttaaata
     7801 tagtcccagt ttcattagac aaaatatcct tagctttttc tttaagtttc catatcatca
     7861 gttatttaat taacgttact cgtggattaa agcggaaata acccgcgctt attattatgc
     7921 atattttacc taagttagta agcgtaggaa acccatgtct tatattatgg aaatatgatt
     7981 aagcagaacc tcttaaggaa aaaacggaat ataaacttgg atttgctgtt taaaattaca
     8041 cagtaacaat tttattatct tattaaaaaa acgtgactca tgttaagtag cttgaatttt
     8101 aattacggat tgtctttaag tagggtataa aactacacga atcaaatcat ttcctcagtt
     8161 gacattacct aaatcaaagc ccgttttaat tttcaaggat agtggtcact ttccttccct
     8221 aacgcttcgc cttcttataa aagcgggtat ttcaaattca tcgctcattt cttcaaccgc
     8281 ggaagtaagt tttgtgacaa cctcctttag ggatgcatcg tctgttaatt tattttcagc
     8341 cctcttac
//
LOCUS       DATDRL010000007         2866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1836304, whole genome shotgun sequence.
ACCESSION   DATDRL010000007 DATDRL010000000
VERSION     DATDRL010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836304"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VI935_00255"
     CDS             complement(<1..585)
                     /locus_tag="VI935_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HLE24059.1"
                     /translation="MNSNKFDVIIVGAGPSGSMTGYYLAKAGVRVAIFDKMKFPREKA
                     CGGGLQVKAVKRIPFDLTSVLRGTLQGITFSFRFDDRYSKKYHLPLVYSVLRIELDNL
                     LLESAKDLGATVYDRANVISVESLKPDLVSVKTEAGEYLCNVLVGADGANSVTRKSVN
                     VLNDYFWQVGLYGEVPEEYLNKNVIDYEQMRVDWG"
     gene            1064..>2866
                     /locus_tag="VI935_00260"
     CDS             1064..>2866
                     /locus_tag="VI935_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24060.1"
                     /translation="MTKTYILRVVPFLVIFLVASFFLQLHSSSKYSSEDKTKPVIGNV
                     ENFVAAYEPWKAMAKKSGADRNLVLPLGYSKALSFKFTNAHGIAKIDLVNGSISVEVS
                     SLPDKEVFDVWLVDNKSSPKDSVKPEPWDKMVRVGSLKHEGTTSRLEAGLDREALKDF
                     KIDLLAVAQDNKGPGEAGLLFGSPSLFQRLYYNEQRGHVAKLGFDASRATSSTDQTSL
                     SFPFNFMVPKPALAVGESTLSMEEMIAFGEELFFEDTFDGNGRTCGTCHPMENNFTID
                     PDFIADLPDDDPLFVAEFNPKLAQLENPALMRQFGLILENVDGLDNPTQKFVMRGVPH
                     TLGLPTSLLRDANIPGVFPAEMTGWSGDGAPGGGALRDFATAAVTQHFTKTLKRKPGK
                     DFRLPNDEELDAMEAFQLSLGRQMDPDLSTLMLQSPDAQTGKNIFVNGTGNMAAPGKC
                     SACHRNGGALNTPNGENRNFNTGVENLPKPTPMAGIPRDGGFGKASVNPAGCTDAPSP
                     NCGFGNGSFNTPPLVEAADTGPFFHNNIIDNIEDAVDFFNSSQFNTPFLISIGANILL
                     DPTQVTQVATFLRVINALENIRSAIQFDNSAMGAS"
BASE COUNT          843 a          665 c          588 g          770 t
ORIGIN      
        1 accccagtca accctcattt gctcatagtc aattacgttc ttgttcaaat actcttccgg
       61 aacctcccca tacaaaccga cttgccagaa gtagtcatta agtacattaa cactttttct
      121 cgttacacta tttgctccat cagctccgac aagaacgtta cataagtact ctccggcctc
      181 cgttttaact gagactaaat caggcttcaa actttctact gatataacat ttgctctatc
      241 ataaaccgta gcgcctagat ctttagctga ttctaagaga agattatcaa gctcaatccg
      301 aagcacacta tagacgagtg gaaggtgata ttttttcgaa tacctatcat caaacctaaa
      361 actaaaagta atgccttgta acgttccacg gagaaccgat gtcaaatcga atggtattcg
      421 ctttacagcc tttacctgaa ggcctccgcc acatgccttt tcacgaggaa acttcatctt
      481 atcaaatata gctaccctta ctccagcctt tgcgagatag taaccggtca tcgaacccga
      541 aggaccagcc ccaactataa tgacatcaaa cttgttgctg ttcatcttac acaattattt
      601 taaatcacct tactgagcga ctgcataaca tctttacatt atatgtatat atcgaataca
      661 gtttaagata aatttgcaaa aacctaaact cagcattcaa tataaagcga cagtccttac
      721 atatcattga gagggggaaa agcccctatg caattttctg cgaagtgtca ttccaacctc
      781 tgcactgaaa tcggtaatgc tttaaacata gctgaaataa agcatcttga cataagaatt
      841 tgtatttgat ataagagtaa tatcaaataa ctagtcctaa ctaaagggcc agccattcaa
      901 aaggaggagg atcaaaacct tagatttagg ccttaaatgg cggttcatgg taactgggtt
      961 gtagagttat aagtttttaa actatttctt ccattacaat ctagggaggg gctttccagc
     1021 ttctatgccc gattagttta aaattaactt aaaggaggat ggaatgacga agacatatat
     1081 ccttagggtt gtccctttcc ttgtaatctt tttagtagct agtttctttt tgcagctcca
     1141 ctcaagcagt aaatattctt cagaggataa gacaaaacct gttataggca atgttgaaaa
     1201 ctttgttgca gcctacgaac catggaaagc aatggcgaag aagagtggag cagacaggaa
     1261 tctagtccta ccgctagggt acagcaaagc tctttctttt aaattcacta atgctcatgg
     1321 aatagccaag atcgacctcg tcaacggctc aatctcagtt gaggtctcaa gcctacccga
     1381 caaagaggtt ttcgacgttt ggcttgtaga taataaaagt tcacctaaag acagcgtaaa
     1441 acctgagcct tgggataaga tggtaagggt tggtagtttg aaacacgaag gtacaacctc
     1501 gaggcttgag gcaggccttg accgtgaggc actcaaggat ttcaagatag atctattggc
     1561 tgtagcgcaa gataacaagg gccctggaga agctggtctt ctcttcggtt cgccaagcct
     1621 atttcaaagg ctttattaca acgaacagcg tggacacgtt gccaagcttg gatttgatgc
     1681 atcacgtgca acaagctcta cagatcaaac tagtttatct ttcccattca atttcatggt
     1741 gccaaaacca gctcttgctg tgggagaaag tacactaagt atggaagaaa tgatagcctt
     1801 tggcgaagag cttttctttg aagacacatt tgatggaaat ggcaggactt gtggaacatg
     1861 tcacccaatg gaaaacaact ttactataga tccagatttt atcgcggatc tacccgacga
     1921 tgatcctctc tttgtggctg aattcaatcc taagctcgcg caactcgaaa acccggctct
     1981 gatgcgccag ttcggcctga ttctcgaaaa cgtcgacggg ttagataatc cgacgcagaa
     2041 gtttgtcatg cgcggtgtac ctcacacact cggtttgcct acatcactct taagggacgc
     2101 aaacattcct ggtgtttttc ctgcagagat gactggatgg tccggtgacg gtgcacctgg
     2161 aggtggtgct ttaagggact tcgccacagc cgcagttaca cagcacttca caaagacctt
     2221 aaaacggaaa ccgggtaagg acttccgttt gccgaacgac gaggaactag atgcaatgga
     2281 agcattccaa ctatcactcg gtcgccaaat ggatccggac ctgagcacgt taatgctaca
     2341 atcaccagac gcacaaaccg gaaagaatat cttcgttaat ggtacgggaa atatggccgc
     2401 cccaggaaag tgtagcgcct gtcaccgtaa cggcggggct ctcaatactc cgaatggtga
     2461 gaatcgtaat tttaatacgg gagtagagaa ccttcccaaa cccacaccca tggcaggaat
     2521 cccacgagat gggggctttg gaaaagcatc tgttaatcct gctggttgta ctgatgcccc
     2581 gtcgcccaac tgcggattcg gcaacggctc tttcaatact cccccattgg ttgaggcagc
     2641 cgacacgggt ccattcttcc acaacaatat aattgataat attgaggatg cagtggattt
     2701 ttttaatagc agccaattta acactccttt tctcatctca attggcgcca atatactact
     2761 tgaccccact caagtcactc aagtcgccac gttccttcgt gttataaatg cacttgagaa
     2821 cattcggtct gctattcaat tcgataacag tgcgatggga gctagc
//
LOCUS       DATDRL010000008         3003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1295187, whole genome shotgun sequence.
ACCESSION   DATDRL010000008 DATDRL010000000
VERSION     DATDRL010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295187"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VI935_00265"
     CDS             complement(<1..233)
                     /locus_tag="VI935_00265"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HLE24061.1"
                     /translation="MDTVTHSLIGALGSRAGFSQRVGRVATIAFVVGAVFPDIDIVVS
                     IFGPEFSLRHHRGITHSVIAAPFFALILGAIIYR"
     gene            complement(344..1471)
                     /locus_tag="VI935_00270"
     CDS             complement(344..1471)
                     /locus_tag="VI935_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HLE24062.1"
                     /translation="MKKDTFLIVDSSENNADLYYRTGFFVPDPVIFIEHNRKRILVLS
                     DLELDRGKKEANVDKVLSLSEYRKKLPSRKRKLSGMTDIVDLVFKELRIKSAVVPGRF
                     PLRYADELRKLGYKISYKSNEPFFEERLRKTPQEIKFISDSLRKTEKAMDLAIRMIAS
                     SEIRGKRLFLKGRALTSEMIKSEINAGLSRLGCVASHTIVACGIHSSMPHHTGEGPLF
                     GNQPIVIDIFPRSQKSGYFGDMTRTVIKGMPSKELEKMYQTVLKGQRLGMSLIKHGAK
                     VKEIHKAIVQFFKKSGFETGTIDGKQQGFIHSTGHGLGLEIHEPPRVGPTEGVLEEGN
                     VVTVEPGLYYEKLGGIRIEDVVVVKKGGCVNLTRYPKKFRV"
     gene            1743..1907
                     /locus_tag="VI935_00275"
     CDS             1743..1907
                     /locus_tag="VI935_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24063.1"
                     /translation="MLETAINKFGLSARAYDRILKVAQTIADLEDSEQVHSHHISEVI
                     QYRTLNRNLI"
     gene            2203..>3003
                     /locus_tag="VI935_00280"
     CDS             2203..>3003
                     /locus_tag="VI935_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24064.1"
                     /translation="MYPSCDINKTLETGSRINIWKQDPSVRGLGVRTTYIHNKVNPGP
                     SDSQIAIKGLPTLYPDSNGDFLFPTPEPRIVEDIYNPPEVTVEEQKFDAAHTYAVVRK
                     VLTMYQRVLGREIKWAWNTGSNDEPLEIYPHANKGRTAYYDRSLKSLRFEYFHILGKY
                     SVFSCRSLDVVSHETGHAMLDALKPSWNITEPGRRISLEIAALQEALCDLTSIFFVLS
                     QLDLVEYIISETKANLHHVDNILAIWSEQLEVIPGYGGARNAINNYKLG"
BASE COUNT          937 a          636 c          522 g          908 t
ORIGIN      
        1 cgatatatta tcgcaccaag aataagggca aaaaatggtg ctgcgattac cgaatgggtt
       61 atccctctat ggtgtcttag agaaaactca ggaccgaata ttgagacaac tatatcgata
      121 tcaggaaaaa ccgcaccgac aacaaaggca atcgttgcaa cccttcctac tctttgggaa
      181 aaacccgccc tcgaacctaa agcccctata agactgtgag taactgtatc caatttttat
      241 aaatgataca cgctgcatga cacatgattt atgatatatc ttgtatcctg catcttgcat
      301 cctgcccccc tggttttttt catttgtgtc aattagtaac tgattaaacc ctaaatttct
      361 ttggatatct ggtgagattt acacatccac ctttttttac aaccacgaca tcctcaattc
      421 ttataccgcc taatttctca tagtaaagtc caggctcaac cgttaccacg ttaccttcct
      481 caagaacacc ttcagtagga cccactctag gcggttcatg tatttcaagt ccgagtccgt
      541 gtcctgtgga atgaataaac ccctgctgct ttccatctat tgtacctgtc tcaaagcctg
      601 atttcttaaa aaactgcact attgctttat gaatctcctt cacctttgca ccgtgtttta
      661 taaggctcat tcctagcctc tgacctttaa gaaccgtctg atacatcttt tctagctcct
      721 tagatggcat gccttttata acggttcttg tcatatctcc aaaataccca cttttctgag
      781 atctcggaaa tatatcaatc acaatcggct gatttccaaa aaggggaccc tctcctgtat
      841 gatgaggcat ggaagaatga attccacaag caacaattgt gtgtgaagca acgcaaccga
      901 gcctggacaa ccctgcgttt atctcacttt ttatcatctc tgatgtgaga gcacgacctt
      961 ttaaaaagag tctttttcct cgaatctcgg atgaagcaat cattcgaata gctaaatcca
     1021 tcgccttctc agtcttccta agggaatcac ttataaactt tatttcttgt ggcgtcttcc
     1081 gcaatctttc ttcaaaaaac ggctcattac ttttgtaact gattttgtag ccgagttttc
     1141 ttaattcatc tgcatatctc agaggaaatc ttccaggtac aactgcactt tttattctta
     1201 attctttaaa aacgagatcc acaatatcag tcatacccga tagcttacgt ttcctcgatg
     1261 gcagtttttt tctatactca gaaagagaaa gaaccttgtc tacgttagct tctttttttc
     1321 ctctgtccaa ttcaagatca ctaagaacga ggattctttt tctgttatgt tcaatgaaaa
     1381 taactggatc agggacaaaa aaccctgttc tataatagag atcggcgtta ttttcacttg
     1441 aatcaacaat aagaaacgtg tccttcttca tagaaacact ataccaaaga gtttgattcc
     1501 ttatcaagaa ataggattac agttacaatc catcaagatc gtttgtaatg aaagatttgc
     1561 caaaaactac gcttaaccac acggacatcc gtgcggttaa caatagcaat aatctcttta
     1621 tagagatagt aaaaatgtac ttaatgaatt aaggataaaa tagcgacata caatcaacaa
     1681 caacgcctta ccgtaacctc tccggcagaa aattttcaag gccggattaa gccgttcaca
     1741 ggctgcttga gactgccata aacaagtttg gcctgagcgc cagggcttat gataggattc
     1801 ttaaggttgc tcaaacaata gctgaccttg aagattcaga gcaggttcac tcccatcaca
     1861 tctctgaagt catccaatat agaaccctta ataggaactt gatttagtaa taaagggttc
     1921 aggactcatc agaaagttgc ccaaatccta atgacttatg acccatacta aaaatgaaaa
     1981 gggacgataa attttttagg ggatccctat tttaaaaaat ttaacaatgt aactgttatc
     2041 ataaagtcgc cttatagtaa aaacatagat cggggcacca aatcctcagc cacttggtaa
     2101 aattggtgtc cctaatttat taaagaggaa aagggattga tattttaata cagatttcat
     2161 ataatggatt ggggatattt aaatatgcct ttaattgttt aaatgtatcc gtcctgcgat
     2221 attaacaaga cacttgaaac aggatctcga ataaatatct ggaaacaaga cccaagtgtt
     2281 cgtggccttg gtgtaagaac tacttatata cataataagg tgaatcctgg accaagtgat
     2341 tctcaaatag ctattaaagg tctaccaacc ctctacccag attctaatgg agattttctc
     2401 tttcctactc ccgaaccacg tattgtagag gatatttata atccacctga agtaactgtt
     2461 gaagaacaaa aatttgatgc agcacatacc tatgcagttg tacgtaaagt attaacgatg
     2521 tatcaacggg tacttggcag ggaaataaaa tgggcatgga acacaggaag taatgatgaa
     2581 cctcttgaaa tttaccctca tgccaataaa ggaaggactg catattatga cagaagtttg
     2641 aaatcattaa ggtttgagta tttccatata ctcggcaagt attccgtttt ctcttgtcgc
     2701 tcattagacg ttgtttcaca cgaaacaggt catgcaatgc ttgatgcttt aaaaccgagt
     2761 tggaatatta cagaaccagg aaggcgaatc tcgcttgaaa tagccgcttt gcaggaagct
     2821 ttatgtgatc tgacatctat ttttttcgtg ctatctcaac tggatcttgt tgaatatatt
     2881 atctctgaaa caaaggcaaa cctgcatcac gtagataaca tacttgctat ttggagtgag
     2941 caacttgagg tgatccccgg atatggtggt gccagaaatg caattaataa ctataaatta
     3001 ggc
//
LOCUS       DATDRL010000009         2708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_510338, whole genome shotgun sequence.
ACCESSION   DATDRL010000009 DATDRL010000000
VERSION     DATDRL010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510338"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VI935_00285"
     CDS             <1..802
                     /locus_tag="VI935_00285"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HLE24065.1"
                     /translation="TKIEVRKWKEYIQGKVKKALTKKSLFALASVSFFAVYREAFETV
                     LFYQALLLQAGNAKDEVIWGLIVGAVLLLCLVVVVFKLGLRIPLKYFFSITSFLLYFL
                     SFVFAGKGIRELQEAGIIGITPLNFIPQIEILGIYPTVETSTVQGILLIALIISLFWL
                     GLIKQEKEKKEIAVSVTRIADDMKSMHEAFEHIKGHIIEWKKCEEIDLEAEELDRKIQ
                     DVITHVDELENKLGDFFDVVSKNRETITAGESQLVTDKPDSQNQPKSS"
     gene            860..1528
                     /gene="queC"
                     /locus_tag="VI935_00290"
     CDS             860..1528
                     /gene="queC"
                     /locus_tag="VI935_00290"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HLE24066.1"
                     /translation="MKAVIIASGGVDSSTLLYKTVKEGCETYGLTFIYGQKHKREIES
                     AKKICEGLNVHHKVIDLSILKDILSGSALTDSGVEIPEVPETAEHYETLKTTIVPNRN
                     SIFLSVAIGYTVSMGANHVYFGAHHSDRGVYPDCRLEFVEAFEHAERLANDSSLLVVA
                     APFVNMTKSEIVKLGVELGVPYKETWSCYKGGEIHCGVCSSCRERKRAFQEAGVVDPT
                     EYAK"
     gene            1760..2173
                     /locus_tag="VI935_00295"
     CDS             1760..2173
                     /locus_tag="VI935_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24067.1"
                     /translation="MTKFSGSFTGKVNWQTKVSLPHLANHHLILEEGSGQQDCTDAKW
                     KGAIVTYWSMITLVEDRGSSQAYYSNVDVDGDRDWGTFGGKITASGDRITLEGTWKLD
                     GGTGKFEGIKGDGNYKGRMVSPTEVKIEWGGEYEI"
     gene            2257..>2708
                     /locus_tag="VI935_00300"
     CDS             2257..>2708
                     /locus_tag="VI935_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLE24068.1"
                     /translation="MKISEIFFSIQGEGVEIGLPTVFVRLFACDLRCTWCDSMYAVEG
                     TDFKEMAIEDVRAEIERYSCKRVCFTGGEPLIQQKELEILAKDLVHYGYEIILETSGH
                     KEPPPVFWTDSCLISMDCKCPGSGMEKRMDFELMEKLRSKDQLKFVIQ"
BASE COUNT          844 a          410 c          669 g          785 t
ORIGIN      
        1 cacaaagatt gaggttagaa aatggaagga gtatattcag ggaaaggtaa agaaagccct
       61 tacaaagaaa agcctatttg cgcttgcaag cgtttctttt tttgctgtat atagggaagc
      121 ctttgaaacc gttctttttt accaggcgct gttgcttcag gctggaaacg ccaaagacga
      181 ggttatttgg ggacttattg ttggagcagt tttgcttttg tgtcttgttg tagtggtttt
      241 taaactcggg cttcgaatac ctcttaaata cttcttcagt atcacgagtt ttcttcttta
      301 tttcctttct tttgtttttg ctggaaaggg aataagggaa cttcaagaag ctggtatcat
      361 cgggataaca cctcttaact ttatccctca gattgaaatt ctcggaatct atccaacagt
      421 cgaaacgtca actgtgcaag ggattttgct tattgcatta attatatcgc ttttctggct
      481 tggattaatc aaacaagaaa aggaaaagaa ggaaatcgca gttagcgtca cgcgaatagc
      541 tgatgatatg aaatctatgc atgaggcttt tgaacacata aaagggcata ttatagagtg
      601 gaagaaatgc gaagaaattg accttgaagc cgaagagctt gatagaaaaa tacaggatgt
      661 aatcactcat gtagatgaac tagagaataa gttaggtgac tttttcgacg ttgtctcaaa
      721 gaatagagaa accattactg ctggggaatc acaattggtt actgataaac ctgatagcca
      781 aaaccaacct aagagctctt aaatcattag tttgtcccat ttcttttaaa catttaatat
      841 taataattag gtgtgttaaa tgaaagcagt tattattgct tcaggagggg tggatagctc
      901 aactcttctt tataaaacgg ttaaagaggg ttgcgaaacc tatggactta cattcatcta
      961 tgggcagaag cataaaaggg aaatcgagtc tgcaaaaaag atttgtgaag gtttaaatgt
     1021 tcatcataaa gttatagacc tttccattct aaaagacatt ctttctggtt ctgctctaac
     1081 agattctggt gtagagattc ctgaggttcc ggaaactgcc gagcattatg aaacattaaa
     1141 gacaacaata gttcctaaca ggaactcgat ttttctcagt gttgcgattg gatacaccgt
     1201 gagcatgggg gcgaaccatg tttacttcgg ggctcatcat tcagatagag gtgtttatcc
     1261 tgactgtagg cttgagtttg tggaggcctt tgaacatgca gagagactgg ctaatgatag
     1321 ttctctctta gtcgtggcag caccgtttgt taatatgact aaatctgaga ttgttaagct
     1381 cggtgttgaa ttgggcgttc catacaagga gacctggtct tgttacaagg gtggagaaat
     1441 acactgcggt gtttgtagtt cgtgcagaga aagaaaaaga gcatttcagg aagcaggtgt
     1501 tgttgaccca acggagtatg caaaatgatt ccactgaaat ttcaggctgt aggtgtaggt
     1561 aaaaaacagt ttctttcaat ggaaatgggg gattggagag aggatttttg ttgctaagaa
     1621 accttatttt gttatatgat aggtttatgc ataagatggg ataattcaag tagaaatggg
     1681 tagggttatt ttatggctgg tggatgactt taccttaaaa agcaccagct aaatttaaaa
     1741 aacaaaaagg agaaaaaaaa tgacaaagtt ttctggatca ttcactggaa aggtaaattg
     1801 gcaaactaag gtatcgttgc cacatttggc taaccatcat ctaattcttg aggaaggatc
     1861 ggggcaacag gattgcacag acgccaaatg gaagggtgca attgttactt actggagtat
     1921 gatcactctt gtcgaagata gaggatcgtc acaagcgtac tacagcaacg tggatgtaga
     1981 cggggatcgg gactggggta cattcggtgg caaaatcaca gcttcaggtg accggataac
     2041 actggagggg acatggaagc tcgatggggg aactggtaag ttcgagggaa tcaagggaga
     2101 tggaaattat aaaggacgta tggtttctcc tacggaggtt aagatagaat ggggcggaga
     2161 atatgaaatt taaatttata gaaatctgat atctgaatta ataggcatga tgaatctatt
     2221 taaggaggtt gtattagagc aagaggaaac aaaagggtga agataagtga gattttcttt
     2281 tcaatacagg gtgagggtgt agagattggt ctgcctactg tgtttgtgcg tttatttgca
     2341 tgtgatctta gatgcacgtg gtgtgattct atgtacgctg ttgaaggtac tgattttaaa
     2401 gagatggcta tagaggatgt aagggcggaa atagaaaggt acagctgcaa aagagtttgc
     2461 ttcacaggtg gcgaaccact gattcaacaa aaggaactgg aaatccttgc gaaggattta
     2521 gtccactatg gatatgagat tatacttgaa acaagcggac acaaagaacc accacctgta
     2581 ttttggactg atagctgtct cataagtatg gattgtaaat gcccgggttc agggatggaa
     2641 aagagaatgg attttgaact catggagaaa cttcgctcta aggatcagct taaattcgtc
     2701 attcagga
//
LOCUS       DATDRL010000010         9003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2878152, whole genome shotgun sequence.
ACCESSION   DATDRL010000010 DATDRL010000000
VERSION     DATDRL010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9003
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2878152"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1869
                     /locus_tag="VI935_00305"
     CDS             313..1869
                     /locus_tag="VI935_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24069.1"
                     /translation="MIKIREKKTRYLNLLTVSALSIALVSLSLSPANSMNEDKDDEKG
                     NFNKSPSSSSSSSSSSSSSSKNSEEDNRFSMKSLEEALPDDGYDSDTEYQRHQEVSER
                     FFRRLTRKPVLRGIIDTFKHEVQNNHPPTQISLDGLSSRTRILLFRELTTYSLAQKLS
                     IPFIFHNKPFQLDLIPDLRQPGNGIITTIGAYTIIYDILHIYSPEYQRQISEKLVNSR
                     SAKGGYHPIILYPTLSKEAATLDLEILNILLDFEVARRLQGTPEEEKTSGFAIKNNTA
                     TESLASFFTTIEKNQKQYKTKVPSSPRVSAERKAEYMLGDKRSYDSVPVASAIVGALT
                     LSKQETPTPLDEFFHATGSITARDGSDFPYGPLNAFQGAPDEAHRMQATKRIIKELRG
                     GEKAHNLKKEDVHQEYLDLFGGASESEGENYDEKSSVTNNKEEIDETPPGKQTTSSSS
                     SSSTFCPKEGEEEKDVKKAQEISSAENEEEETADAIQFSIQDNANEKGIPLVIPSSKT
                     ESPKKEEPEK"
     gene            complement(2235..2591)
                     /locus_tag="VI935_00310"
     CDS             complement(2235..2591)
                     /locus_tag="VI935_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLE24070.1"
                     /translation="MMEYKGYVGNVTFDDDVGIFHGDVVNTRDVITFQGRTVDEIRKA
                     FHESVDEYLDWCKERNKTPDKPFSGKLIVRIPPALHRSIFLSAKQEGKSLNRWIEDHL
                     MSSFPPTSEESLTHHH"
     gene            complement(2588..2842)
                     /locus_tag="VI935_00315"
     CDS             complement(2588..2842)
                     /locus_tag="VI935_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HLE24071.1"
                     /translation="MRRKHARTLNAIFSDPIQSGILWRDIETMLEALGAEIYERRGSR
                     VCVSLNGIDAVFHRPHPRKETDKGAVSSMRRFLKEAGVEL"
     gene            complement(2896..3114)
                     /locus_tag="VI935_00320"
     CDS             complement(2896..3114)
                     /locus_tag="VI935_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLE24072.1"
                     /translation="MITYKGYTGSFIFDEKENLFKGKVANSNYPISFQGKSIKEMKQT
                     FYDSVDEYLHWCKKYGKIPEKPSTDSTE"
     gene            complement(3243..3950)
                     /locus_tag="VI935_00325"
     CDS             complement(3243..3950)
                     /locus_tag="VI935_00325"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HLE24073.1"
                     /translation="MFDKRKVITTPQKYPWSIHGYLRCVFANGTVHRGTGTMISKNCL
                     LTAGHCLYNEDAEEEDFRLPISLTYFSGLHDNNYFLKTEYRKVFIHTAYKESNDESFD
                     FGVVQLERDIGEQTGWASLKTLDDKALHSLKINVTGYPVHVSPYSVLIKKPAYSMYTM
                     EGAVELVKKHKFYYKLDTSGGQSGSGVWTLDKNGNVLCVGIHVTGSKLEGNGAVRVNE
                     ENLETITYWLDMMRQNP"
     gene            complement(4052..4438)
                     /locus_tag="VI935_00330"
     CDS             complement(4052..4438)
                     /locus_tag="VI935_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24074.1"
                     /translation="MKNFVLPSIGILAFSISAVYAGGGFSRLAIENSYPMVRHRISVK
                     PNWFMIYSSRKYGTLRPTIGSKPTKPMPEKPFYEEMPKKVRFLDEQHERYVPGTGSVK
                     ETSKDSLPNVEKKDPNTTIITPQKKR"
     gene            4780..4962
                     /locus_tag="VI935_00335"
     CDS             4780..4962
                     /locus_tag="VI935_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24075.1"
                     /translation="MTQNSYLTRLFLEPTFSQVVPIQMSENGNVLVQSSGDNVAAGNP
                     VGFVCENKSETTSIGW"
     gene            4995..5381
                     /locus_tag="VI935_00340"
     CDS             4995..5381
                     /locus_tag="VI935_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24076.1"
                     /translation="MNSYNYRQRQRVKDTATLSSKGTATGNARIGCSCIANHRVAGGE
                     RAPKFCVSLQSGTWQACRSPGNRKMYRKVTLMGLQVEEVVKSKGLPVMGGIQSFLWRE
                     SEPTYHRSFIARKFEEPLRRNANDNS"
     gene            5368..5538
                     /locus_tag="VI935_00345"
     CDS             5368..5538
                     /locus_tag="VI935_00345"
                     /inference="COORDINATES: protein motif:HMM:NF025039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase N-terminal
                     domain-containing protein"
                     /protein_id="HLE24077.1"
                     /translation="MTIASPIGASPDQISTLSWDQIPWKQAEKTVQRLQMRIAKAIRL
                     WSGVSNPTLLKA"
     gene            complement(5879..6832)
                     /locus_tag="VI935_00350"
     CDS             complement(5879..6832)
                     /locus_tag="VI935_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HLE24078.1"
                     /translation="MITCINLGKSLSLLLDAATEIQMDPEPTEQDKAFLTRQLVQVTL
                     PHSSPGNVPIWKRRNGNLTLSIRPGWNHEKDQPIGYPFGSIPRLLLFWITTEALRTQS
                     RKLELGESLAGFMRDIGLDPSTGGGKRGDGRRLQNQMERLFRATISLEMMDTKDKNVI
                     GKRWIDMQVAPEGELWWNFKNPGQATLFGSWIELGEKFYEAIIASPVPLDKRALRALK
                     RSPLALDLYAWATYKTYSLNHHKKVEQFIPWHAFMAQLGAEYNDHKNFKKKAKAAFKK
                     VRSVYPGLEMEEVDGGFIIRQGKTAIRQKQSRRKLSTKISG"
     gene            complement(6880..7122)
                     /locus_tag="VI935_00355"
     CDS             complement(6880..7122)
                     /locus_tag="VI935_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24079.1"
                     /translation="MTQKPSLHEALHKSSAQRKKIIEAETSSARETKKLIVVIPTEAH
                     LQIDQMKRDLRRRSMKDVVTEALNDLFKKYEYPPIA"
     gene            complement(7085..7747)
                     /locus_tag="VI935_00360"
     CDS             complement(7085..7747)
                     /locus_tag="VI935_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLE24080.1"
                     /translation="MKVLAIISQKGGTGKTTLALNLAVASELSGNPAAVFDLDPQASS
                     MSWKDNRPDESPAVVSLHSARITHYLDTARQSGAKLVIFDTAPHSQKDALEAAQASDL
                     ILIPCKPSLIDLRAISSSVKIAQLAGKPAVAILTQTQTRGSLTDEAVEAIKSYEVSIA
                     PVTIGNRMAFIHAFTAGRGVLEIEPQSKAGEEITSLFSFITNLMENKDNDAKAKPSRS
                     AA"
     gene            complement(7851..8408)
                     /locus_tag="VI935_00365"
     CDS             complement(7851..8408)
                     /locus_tag="VI935_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HLE24081.1"
                     /translation="MHIGYARVSTSDQNINLQKEALEIAACQRIYEDHTSSTREDRPG
                     LHKALEQLREGDTFVVWKLDRLGRNVKNLIELVTSLEKKNIHFKSLTDHIDTSNPSGR
                     FFFHVMASLSQMERELIVERTRAGLKAAKLRGRVGGRKRRMTPSKIDSAKQLLANGMP
                     PKEVANNLGVSIPTLYRWIPASSLP"
     gene            complement(8419..8742)
                     /locus_tag="VI935_00370"
     CDS             complement(8419..8742)
                     /locus_tag="VI935_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24082.1"
                     /translation="MSRSYKKTPIMENTTCHSERLDKSLWLRLGRRKERINLDALNAE
                     NKDDYCTTHQREVSNPRYMGKDGKNYFSPEEQAQFVKRMANTIGQDSQERASLKRLLY
                     KLMGK"
BASE COUNT         2628 a         1800 c         1772 g         2803 t
ORIGIN      
        1 ctaacacatc ggcctcacca tacgacatag tggttcggct cgcgaacatt ccctacaatg
       61 caatttccat aagggtccct cctaaaattc tcggactttc ctttgctcgt tcctgctcgc
      121 ctgcttcctc cgtaaaaatg gaaggctatt aatcccacaa aagcttgtga gaaaatcaac
      181 tgtggccgtt gtgtggaaat attcacagtg tctaaaaatc agggccttga cttatttaaa
      241 ttaagagtaa atactttttt gttagctaat caagattagc taactaataa gacacaaaaa
      301 acaggagaat ttatgattaa aattagggag aaaaaaacaa gatatttgaa cttattaact
      361 gtttctgctc tttcaattgc attggtttct ctatcattat cgccagcaaa ctcaatgaat
      421 gaggataagg atgatgagaa aggtaacttt aataaatccc catcctcttc ttcttcatct
      481 tcttcatctt cctcatcttc ttctaaaaat tcagaagaag ataataggtt ttctatgaaa
      541 agcttagaag aagcacttcc tgatgatggt tatgacagtg atacagagta tcaacgacat
      601 caagaggtaa gtgaacgttt ctttagacga ctcacgcgca agcctgtgtt aagaggaata
      661 atagacacgt ttaaacacga ggtccaaaac aaccatcctc caacgcaaat tagtttagac
      721 ggattatctt ctcgcacgag aatattgttg ttcagagagt taaccacgta tagtttagca
      781 caaaagctat ccataccttt tatatttcat aataaaccat tccaacttga tttaatccct
      841 gacctccgac aaccagggaa tggaataatt actactattg gtgcatatac aattatatat
      901 gacatattgc atatttattc tcctgaatat caacgacaaa tttcggaaaa actcgtaaat
      961 tctcggagcg ctaagggagg ttatcatcct attattcttt atcctactct ttcaaaagaa
     1021 gctgcaactt tggatttgga aattttaaat attctcctag attttgaagt cgcacgacga
     1081 ttgcagggga ctccagaaga ggagaaaacc tccgggtttg ccataaaaaa taacacagca
     1141 acagaatctc ttgctagttt ttttaccact attgagaaga atcagaagca atataaaaca
     1201 aaagtgcctt ctagcccgag agtgtctgct gaaaggaaag ccgagtatat gttaggagac
     1261 aagcgttcct atgatagtgt acctgtagcg tcagctatcg ttggggcatt aactttatca
     1321 aaacaagaaa ctccaacccc tcttgatgag ttttttcatg ccacaggctc tattactgct
     1381 cgagatggct ccgactttcc atatggtccc cttaatgcat ttcaaggggc tcctgatgag
     1441 gctcatcgaa tgcaagcaac aaagagaatt attaaagagc taagaggggg agagaaggcg
     1501 cacaacttaa aaaaggaaga tgttcatcaa gaatatctag atctttttgg aggggctagt
     1561 gaaagcgaag gtgagaacta cgatgaaaaa tcttcagtta ccaataataa agaagaaatt
     1621 gatgagactc ctccaggaaa gcaaactaca agttcttcaa gctcttcttc cactttctgt
     1681 cctaaagaag gagaagagga aaaagacgtc aagaaggcac aagaaatttc tagtgctgaa
     1741 aatgaggaag aagaaacagc agatgcaata caattttcaa ttcaagacaa cgcaaatgaa
     1801 aaagggatac cattagtaat accatcatca aaaactgaat cgccaaaaaa agaagaaccg
     1861 gagaaatgat tgattctcaa gttcattaat ttctagctgt aacccagggc tttgaatggg
     1921 gagagataga gaaaacaata agagtctttt gaccagagat ttcctacaca acgcaaataa
     1981 acgcgttttt atggccctct ggcgtgttta attctttttt acgcattctt ccgcgaaaaa
     2041 atcattttcg cttctgtgag acgatcctag gaggttttag aggtatttta tgctaggggg
     2101 aaccctcaag aatttatcat ccgaaggagg tgcacttatg cgctcataaa atgagcgtag
     2161 tgctttatgg ccacgggcgt taaaagttat gacaaagcaa gaataacaag agccgctgag
     2221 acttataaat catcttaatg atgatgagta agcgattctt cagatgtagg ggggaacgag
     2281 ctcatcaaat gatcttcgat ccatctgttc aagctttttc cttcttgttt tgcagaaaga
     2341 aagatagaac gatgaagagc agggggaatc cgaacaataa gctttcctga aaaaggtttg
     2401 tctggcgttt tatttctctc tttacaccag tcaagatact catcaacaga ctcatgaaaa
     2461 gcctttcgaa tttcatctac agtccttcct tgaaaggtga tgacatcgcg tgtattaaca
     2521 acatctccat gaaaaatgcc tacgtcatca tcaaaagtaa cgtttccaac atagccttta
     2581 tattccatca tagttcaact cctgcttctt ttaaaaaccg tctcatagaa ctcacggcac
     2641 ctttatctgt ttctttacga gggtgaggac gatgaaagac agcatcaatt ccattcagag
     2701 aaacacaaac acgagagcct cttctttcat atatctctgc ccccaaagct tctaacattg
     2761 tctcaatatc acgccataat attcctgatt gaataggatc agagaaaata gcattcaagg
     2821 ttctggcgtg ttttcttctc attctttctt aatagtatca ctttctgata ctatcttcaa
     2881 gatgaaaaag catgtttatt ccgttgagtc tgttgaaggt ttttcaggga tcttgccgta
     2941 ttttttacac cagtggagat actcatcaac ggaatcatag aaggtttgtt tcatttcttt
     3001 tatggatttt ccttggaaag agattggata attagagttt gcaacctttc ctttgaagag
     3061 attttctttt tcatcgaaga taaaagatcc agtatagcct ttatatgtga tcataaaaat
     3121 tgtgtgatgt gtatgacttt tttctttagt ttaatagggt cgaatctaat cttagtagcc
     3181 tctttctttt ctacttctta tagatgaagg atagcaaacc tgtgtttctt aagcaaggga
     3241 gcttatggat tttgtctcat catatccaac caatatgtaa ttgtttctaa attttcctcg
     3301 tttaccctaa cagcaccatt tccttctaat ttagagcctg taacgtgaat tcctacacat
     3361 aagacattac cgttcttgtc taaagtccaa acaccagatc cactttgtcc acctgaggta
     3421 tcgagcttgt agtaaaattt atgctttttt accaactcaa cagcaccttc cattgtgtac
     3481 atggagtacg ctggtttttt tattagaaca ctataaggac tcacatgtac aggataccct
     3541 gtaacgttaa ttttgagaga gtgtaaggct ttatcatcca atgtttttaa agaggcccaa
     3601 cctgtttgtt ctcctatatc cctttctagc tgtacaactc caaaatcaaa actttcatca
     3661 ttggactctt tataagcagt atgaataaaa acttttcgat actctgtctt caaaaagtaa
     3721 ttattgtcgt gcaatccaga aaaatacgta agacttatag gaagtctaaa atcttcctcc
     3781 tcagcatctt cgttatataa acagtgacca gcagttaaca gacaattttt gcttatcatt
     3841 gttccagttc cacgatgcac tgtaccatta gcaaatacac aacgaagata cccatggata
     3901 ctccatgggt acttttgggg cgttgtaata acctttcgtt tgtcaaacaa ggcgtctacg
     3961 acattatagc taagacctac aaagggacta ctcagaacag caaactgatc atgcttgcct
     4021 ttttggggct gaccaaactc ttcaggaaga gttacctttt tttttgagga gtgatgattg
     4081 tcgtattagg gtcttttttc tcaacatttg gcagagaatc ttttgaagtt tcttttacgc
     4141 tgccagttcc gggaacatat ctttcatgct gctcatctag aaatctaacc ttttttggca
     4201 tctcttcata aaaaggtttt tcaggcattg gtttggtagg ttttgaacct attgtaggcc
     4261 ttaatgttcc atattttcta gaagagtata tcataaacca attaggtttt actgaaattc
     4321 tatgtcttac catagggtat gagttctcaa tagctaatct tgaaaaacca ccacctgcat
     4381 agacagcaga aatactaaat gcaaggatac caatagatgg taaaacaaag tttttcatga
     4441 gagagctcct atagaattac aaaatatatc ataaccctga tatattaaat tttcaatgaa
     4501 taattaatga aattgggttt ttcaactaag acctgagcgt ttgctagttt agtgtgggcg
     4561 gtaaatttcg caactgcctt gtttttgttt cttgatgagc tttgttagat taatagtctt
     4621 tcatttttta tggagaaaat aacgagcgga agtgaataaa ggaaagtccg aatattttag
     4681 aggcgcgctt atacgtcggg aaatccgacg cgcactttgt tgcaagcaac tgtgcgacac
     4741 gaagtcgctt tgtagaatgt tcaaaaggag aattgagaaa tgacccaaaa tagttatctc
     4801 acaaggctct ttcttgaacc aactttttcg caagttgtcc ctatccagat gagcgagaat
     4861 ggcaacgttc tggttcaaag ctctggagac aatgtagccg caggaaaccc tgtaggattt
     4921 gtttgtgaga ataaatctga aactaccagt atcgggtggt gaggaactag ggaagcagaa
     4981 tatggagaac ggatgtgaac tcttataact atcgtcagcg acaaagagtg aaagatactg
     5041 ccacactctc gtcaaaaggc actgcaactg ggaatgcaag gatcggttgc tcctgcatcg
     5101 cgaaccacag agttgccggt ggagaaagag ctcctaagtt ctgtgtaagt ctacaaagtg
     5161 gaacgtggca agcctgtaga tctcctggca acaggaaaat gtaccgcaag gttacattga
     5221 tgggtctgca ggtagaggaa gtggtaaaaa gcaaaggcct tcctgtaatg ggagggatac
     5281 agagttttct ctggcgcgaa agcgagccca cttaccacag gtcttttatc gcaagaaagt
     5341 ttgaagaacc cttaaggagg aatgcaaatg acaatagcta gtcctattgg tgcctctcct
     5401 gaccagatat caaccctctc ctgggatcaa atcccttgga aacaggctga gaaaacggtt
     5461 caacggcttc agatgcgtat tgcgaaggcc ataagattat ggtcgggtgt gagcaatccc
     5521 accttactaa aggcttgagc cgtatgatgg aaaactatca cgtacggttc ttaggggacg
     5581 ggagaagcag caatgcttct cttgttaccc gaccagataa attttgagaa ttacatcttt
     5641 tttttcttat aaaaaacaaa ttctttttgt tgttgtgcaa gcgagctgtg taaaacaatt
     5701 ttagttttgc acagctaagc gtcgcaaact acaggttctt atactatagg atatatctat
     5761 aggaaggggg taccgtttca ccgtataaga ggggtaccgt ttcaccgtgg ataactcaaa
     5821 attccgttga aataattgaa ttaattgggg ggtaccgttt caccgtggat aacttatttt
     5881 acccactgat ttttgtggat agtttgcgac gtgattgttt ttgacgtatt gcagtttttc
     5941 cctgtcgaat tatgaatcca ccatcaacct cctccatttc taatccaggg tagacagagc
     6001 gcactttctt gaatgcagct tttgcttttt tcttgaaatt cttatggtca ttatattcag
     6061 ccccaagttg tgccataaat gcatgccatg gaataaactg ttccactttt ttatggtgat
     6121 taagagaata ggttttataa gttgcccagg cgtacagatc taaggctagt ggagagcgtt
     6181 taagggctct taaagcccgt ttatcaagcg gaacaggtga agctataatt gcttcataga
     6241 acttttcacc aagttcaatc caagatccaa aaagcgtcgc ctgaccaggg tttttgaaat
     6301 tccaccataa ttcgccttct ggagctacct gcatgtcaat ccaacgtttt ccaattacgt
     6361 ttttatcttt tgtatccatc atctccaagc taatagttgc ccgaaaaagc ctctccattt
     6421 ggttttgaag cctccgtcca tcaccacgct taccaccacc cgttgaagga tcaagaccaa
     6481 tatcacgcat gaatcctgct aggctttctc ctagctcaag tttgcgactt tgtgttcgta
     6541 gggcttctgt tgttatccag aataacagaa gtcttggaat ggaaccaaat ggataaccga
     6601 taggctgatc cttttcatga ttccatccag gtctgattga taaggttaaa tttccgttcc
     6661 tgcgtttcca tataggtaca ttgccgggac tggagtgcgg aagagttacc tgtacaagtt
     6721 gtcgagttag aaatgcttta tcttgctcag ttggttctgg atccatttgg atttcagttg
     6781 ctgcatcaag taagagagaa aggcttttcc ctagatttat acaggttatc atttttatat
     6841 tttcatatct tcatatttat gattttgcat aaaactatat taagcaatcg gcggatactc
     6901 gtatttttta aacaagtcgt tcaaggcttc tgttacaaca tccttcatcg atctacgcct
     6961 caaatcacgt ttcatttggt caatctgtaa atgtgcctct gttggaatta ctacaatcag
     7021 ctttttagtt tcccttgctg atgaagtttc tgcttcgatg attttcttcc tctgtgctga
     7081 tgatttatgc agcgcttcgt gaaggcttgg cttttgcgtc attgtccttg ttctccataa
     7141 ggttggttat aaaagaaaaa agagaagtaa tttcctctcc tgctttactc tgcggctcga
     7201 tttccaaaac accccttccg gcagtaaagg catgaataaa tgccatacgg ttgccaatcg
     7261 ttactggggc aatagaaact tcgtagctct taattgcctc tacagcctca tctgttagtg
     7321 agccgcgagt ctgtgtttgg gttagaatag caactgccgg cttgccagcc aactgtgcga
     7381 ttttcacgct tgagctgata gctctcaaat ctatcagtga cggcttacag ggaatcaaga
     7441 ttaaatcaga ggcctgagcg gcttccagtg catctttttg agaatgaggg gccgtgtcaa
     7501 atattacaag ttttgccccg ctctgacggg ctgtatcgag ataatgtgtt atacgggctg
     7561 aatgtagaga tacgacagct ggtgattcat caggtctatt gtctttccaa ctcatagatg
     7621 atgcttgcgg gtctaggtca aagactgcgg ctgggtttcc tgatagttca cttgcgacag
     7681 ccagattaag agcaagtgtt gttttccctg tgccgccttt ttgcgagatg attgccaaaa
     7741 ctttcatatt tacacatttc catatcatta tagtttcata aatcaacaat gccacattta
     7801 tgaaaaatta caatagtatt cttttaaggt atcacctcac taaacgcacg ttaaggaaga
     7861 gatgaggcag gtatccatct atagagtgtt ggaattgaaa caccgaggtt gtttgctact
     7921 tccttagggg gcattccatt ggccaacaac tgcttcgctg agtcaatttt gctgggggtc
     7981 atgcgtcgtt ttcgacctcc aaccctccct cgtagttttg ctgcctttaa tcctgcacgt
     8041 gttcgttcaa caatgagttc tctctccatt tgagaaagac tggccatcac atgaaaaaag
     8101 aacctccctg aggggttact ggtatcaata tgatctgtga gactcttaaa gtgaatattc
     8161 ttcttttcaa gagaggtgac aagctcaatc aaattcttaa cattgcgccc cagacgatca
     8221 agcttccaga caacaaaggt atctccttca cgaagttgtt cgagggcttt atgtaatcca
     8281 gggcggtctt ctcgtgtgct gcttgtatga tcctcataga ttctttgaca agccgcgatc
     8341 tctaaggctt ctttttgtaa attaatgttt tgatcacttg ttgaaactct ggcgtaacca
     8401 atatgcattc ttaacccttt attttcccat tagcttataa agtaggcgtt ttaatgaggc
     8461 tcgttcttga gaatcttgtc caattgtatt ggccattctt tttacaaact gcgcttgttc
     8521 ctctggggag aagtaatttt taccgtcttt tcccatatac cgggggttac tgacttctcg
     8581 ttgatgggtc gtgcaataat cgtccttatt ttctgcattg agagcgtcga gatttatccg
     8641 ttccttccgt ctccctaatc taagccaaag gcttttatct aacctttcac tatggcaggt
     8701 tgtattttcc atgataggtg tttttttgta gctacgactc atagatggtt tttccttctc
     8761 aaaacccatc atcagagtaa tctaataaga aaatactttc aagatgggtt tttgagaatt
     8821 ttgaagtcaa agatctgggt ctcaattcct ttggtgattt agattatcac aaacgaacga
     8881 ttttgataaa aggaggatgt ttttacatcg ttgattatta tgaataaacg gaaacttttc
     8941 ttttcttgat gcaaaattcc ggctgtaaaa tactattgac ttttatgaaa aggaggcctt
     9001 cac
//
LOCUS       DATDRL010000011        13232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_233259, whole genome shotgun sequence.
ACCESSION   DATDRL010000011 DATDRL010000000
VERSION     DATDRL010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13232
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233259"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VI935_00375"
     CDS             complement(<1..386)
                     /locus_tag="VI935_00375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812977.1"
                     /note="catalyzes the transcription of DNA into RNA using
                     the four ribonucleoside triphosphates as substrates.
                     Dimerization of the alpha subunit is the first step in the
                     sequential assembly of subunits to form the holoenzyme;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HLE24083.1"
                     /translation="MKDFQRNWQELIRPKRLEKDEKDSSMVYGKFICEPLERGYGVTF
                     GNALRRVLLSSIQGPAITSLKIEGVLHEFSTIPGVKEDVTEIVLNLKGIELKMHTYEP
                     QLITVSAMGECDVKAGDIITGHQIEIL"
     gene            complement(457..1089)
                     /gene="rpsD"
                     /locus_tag="VI935_00380"
     CDS             complement(457..1089)
                     /gene="rpsD"
                     /locus_tag="VI935_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HLE24084.1"
                     /translation="MAKYTGAVCRLCRREGIKLFLKGDRCYTEKCAVERRPDQVPGEH
                     GLRRPKFSEFGIRLREKQKVRRIYGLTERQFRRYFERASRVKGITGELLISLLERRLD
                     NVVYRLGFAGSRKEARELVRYGHILINGRKLDIPSYQTKLSDVIEVKHGSQKLDRINK
                     SLEILARRGLPDWLELEKESYKGVVKRLPQRIDVTFPIEEQLIVEFYSRV"
     gene            complement(1249..1635)
                     /gene="rpsK"
                     /locus_tag="VI935_00385"
     CDS             complement(1249..1635)
                     /gene="rpsK"
                     /locus_tag="VI935_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HLE24085.1"
                     /translation="MLAKKKAAKKSKKFADQGVAHIQATFNNTIITISDNQGNVIAWS
                     SSGAKGFKGTRKGTPFAAQIAAEDAARKVVAHGMKSLVVYVKGPGPGREPAIRSLQSA
                     GLRITMIRDVTPIPHNGCRPAGRRRV"
     gene            complement(1774..2154)
                     /gene="rpsM"
                     /locus_tag="VI935_00390"
     CDS             complement(1774..2154)
                     /gene="rpsM"
                     /locus_tag="VI935_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HLE24086.1"
                     /translation="MPRIAGVDIPPNKRVEVGLTFIYGIGRLTSRKILAKAEVDLNRR
                     SKDLNEQEIAKIRDVIERDYTVEGELRKEVQTNIKRFIEIGCYRGTRHKLGLPVRGQR
                     TRSNARTRKGPRGTAITKRKKLKG"
     gene            complement(2166..2279)
                     /gene="rpmJ"
                     /locus_tag="VI935_00395"
     CDS             complement(2166..2279)
                     /gene="rpmJ"
                     /locus_tag="VI935_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HLE24087.1"
                     /translation="MKVRASVKKICDKCKVIKRRGAIRVICNNKKHMQKQG"
     gene            complement(2310..2531)
                     /gene="infA"
                     /locus_tag="VI935_00400"
     CDS             complement(2310..2531)
                     /gene="infA"
                     /locus_tag="VI935_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HLE24088.1"
                     /translation="MPKEERIEFEGTVIEALPNAMFRVEIEGGPKILAYVSGKMRTHF
                     IRILPGDKVKVELSPYDLTKGRITYRLKK"
     gene            complement(2542..3306)
                     /gene="map"
                     /locus_tag="VI935_00405"
     CDS             complement(2542..3306)
                     /gene="map"
                     /locus_tag="VI935_00405"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HLE24089.1"
                     /translation="MIFLKSEEELEKIRRASKIVVEVLERLSEETKPGVSTWGLDKIA
                     EELTFKKGAIPAFKGYRGYPASVCFAVNEEVVHGIPSKKKILKEGDIVSLDFGAYLDG
                     FYGDSAITVPVGKVSQLADSLLRVTRNSLYEGIKMARASNRLFDISQAIQNCVENEGF
                     SVVRAFVGHGIGRKLHEDPQVPNFVPENGHWGKGIRLKPGMVIAIEPMVNVGRPDVKV
                     LGDGWTAVTVDGTLSAHFEHTVAILEDGPKILTEFR"
     gene            complement(3307..3957)
                     /locus_tag="VI935_00410"
     CDS             complement(3307..3957)
                     /locus_tag="VI935_00410"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HLE24090.1"
                     /translation="MRLVIFGPPGAGKGTQAAKISEDYKVLHISTGDILRASVKEGTE
                     LGKLAKTYMDKGELVPDNVIIGIIKERIAMQDSQKGFLLDGFPRTIEQAEALERMLSK
                     EGQRIDSVISIEVDDEEIIKRISGRRVCEKCGTMYHIIFDPPTNMGYCNKCGGGLYQR
                     DDDSEDVIRTRLQVYRRQTEPLKKFYKKVGILKRVDGLGTIDDVFERIEKILKGIA"
     gene            complement(3961..5280)
                     /gene="secY"
                     /locus_tag="VI935_00415"
     CDS             complement(3961..5280)
                     /gene="secY"
                     /locus_tag="VI935_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HLE24091.1"
                     /translation="MSRNIGAIPKIPELNRRLLFAALMLVVYRLGVFVPTPGIDPEEL
                     IKLFEQTKGTIFDILNLFSGGALERASIFALGVMPYITSSIIMSLLVKAFPTLEAMQK
                     EGEAGRRKINQYSRYGTVLICIIQGLMLAITLEGGLGSGASVVRDPGWMFRFTTVMTL
                     TAGTLFVMWIGEQISERGIGNGISLIIAASIITRVPAALWDSVRLIETGELTVFGMLL
                     LFAFMAFIVGLIVYFERSYRKIPVQYPRRVVGRKVYGGQASHLPLKVNTAGVIPPIFA
                     SSLMIFPATILSFVNIPVLRDMTDLFLSQGFIYNAMYAGLIIFFAYFYTAVIYNPDDL
                     ADNLRKNGGFIPGIRPGKKTAEYIEKILGRITFIGAIYVAAVCVLPSILQGAPFNIPF
                     FFGGTSLLIVVGVTLDTVQQIESFLITHSYEGFIRKRGMKGPRRYGI"
     gene            complement(5277..5708)
                     /gene="rplO"
                     /locus_tag="VI935_00420"
     CDS             complement(5277..5708)
                     /gene="rplO"
                     /locus_tag="VI935_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HLE24092.1"
                     /translation="MLNRLSSPEGAAKNRKRLGRGPGCHGKTSGKGHKGQRSRSGGRV
                     SPWFEGGQTPLKMRTPKRGFRSSQKTHFDIVNVKDLNNFKDGEVVTPEELVKLGFVSG
                     KNPVKILGDGEINAALTIRAHVFSKEAKRKIEERGGKAEIL"
     gene            complement(5870..6394)
                     /gene="rpsE"
                     /locus_tag="VI935_00425"
     CDS             complement(5870..6394)
                     /gene="rpsE"
                     /locus_tag="VI935_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HLE24093.1"
                     /translation="MPKERVDPSGLELKEKIVHVRRVAKVTKGGKRFHFTVLVVIGDN
                     KGIVGTALGKSNEVPDAIRKAIEKAKKNLIRVPRHGSTIPHDIIGEQSASRVILRPAA
                     PGTGVIAGGAVRAVVELAGIQDILSKVLGSTNPLNVVVAAIKGLSSLKIPENVARLRG
                     RETKDLDLPKYYQE"
     gene            complement(6435..6809)
                     /gene="rplR"
                     /locus_tag="VI935_00430"
     CDS             complement(6435..6809)
                     /gene="rplR"
                     /locus_tag="VI935_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HLE24094.1"
                     /translation="MIRKKTRKERRDIRHRRVRRKLRGTSTPRLSVFKSSKHIYSEII
                     DDIKGHTLLAASTLTPKIRTTLNNEGKKNKVETAKLVGKYIGETALSRGIKKVCFDRG
                     GYLYHGRVKALADGAREAGLEF"
     gene            complement(6806..7354)
                     /gene="rplF"
                     /locus_tag="VI935_00435"
     CDS             complement(6806..7354)
                     /gene="rplF"
                     /locus_tag="VI935_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HLE24095.1"
                     /translation="MSRIGRRSIFIPSGVKVNIKDGLVEVLGPKGKLARHIPFGIEVK
                     ADVSQVSVERKSDNKELRALHGLTRALLSNMIFGVTEGFSKSLDIVGVGYKAELLGKS
                     NLKFSLGYSKPVEFSLPEGLAVRIEERGTRMIIQGIDKEVVGETAARIKRLRLPDSYK
                     GKGIRYSDEILKLKPGKAGAKK"
     gene            complement(7365..7763)
                     /gene="rpsH"
                     /locus_tag="VI935_00440"
     CDS             complement(7365..7763)
                     /gene="rpsH"
                     /locus_tag="VI935_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HLE24096.1"
                     /translation="MVMTDPIADMLTRIRNALKARFGFVDVPASRIKFDIARVLKEEG
                     YISDFKVLEDGIKKIIKIALKYTPQKESMITEIRRVSKPGHRVYVNKNEIPRIRGGLG
                     IYILSTPKGIMTGIRARRSGIGGEVICTIW"
     gene            complement(7848..8033)
                     /locus_tag="VI935_00445"
     CDS             complement(7848..8033)
                     /locus_tag="VI935_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HLE24097.1"
                     /translation="MATKSMMEKLRSKPKFRVRHRNRCPLCGRPRAYLRIFNMCRLCF
                     RQAASFGKVPGVKKASW"
     gene            complement(8202..8756)
                     /gene="rplE"
                     /locus_tag="VI935_00450"
     CDS             complement(8202..8756)
                     /gene="rplE"
                     /locus_tag="VI935_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HLE24098.1"
                     /translation="MVPRLKQYYKDKVTKLLKERFGYKNLMEIPRIDKIVINMGLGAL
                     SDGGKENKIIDDAVQEISIITGQKPVVTRARKSIAGFKLREGATIGCMVTLRGERMYE
                     FFDRLVNLALPRVRDFRGVSPQAFDGNGNFTLGLREHTIFPEIEYGKVEKVKGVNITI
                     VTTAKTNEESKGLLKDMGMPFRER"
     gene            complement(8756..9094)
                     /gene="rplX"
                     /locus_tag="VI935_00455"
     CDS             complement(8756..9094)
                     /gene="rplX"
                     /locus_tag="VI935_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HLE24099.1"
                     /translation="MEVTQGKEVHIKSGDTVYVISGKEKGKTGKVLRILRDKRKAVVE
                     KLNMVKRHARPSQKNPGGGIIEKEAGIHISNLMIYCSKCSRPVRIGRKRLSSGEKIRY
                     CRRCDEEIKS"
     gene            complement(9098..9466)
                     /gene="rplN"
                     /locus_tag="VI935_00460"
     CDS             complement(9098..9466)
                     /gene="rplN"
                     /locus_tag="VI935_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HLE24100.1"
                     /translation="MIQPTTILQAADNTGAKRLFCIRVMGGSNRKYAGLGDVIVVSVK
                     EAIPNSKVEKGDVHRAVIVRTRKEQRRSDGSYVRFDDNAAVLITKENEPIGTRVFGPV
                     ARELRSKGFMKIISLAPEVV"
     gene            complement(9463..9771)
                     /gene="rpsQ"
                     /locus_tag="VI935_00465"
     CDS             complement(9463..9771)
                     /gene="rpsQ"
                     /locus_tag="VI935_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HLE24101.1"
                     /translation="MSRGKVKTRVGVVTSNKMNKTVVVDVTQIVHHPKYGKHVKRISR
                     FKAHDEKNDCRIGNKVFIIETSPLSRTKRWRVSKILERATLLEAELPKEDLGEGGKGE
                     "
     gene            complement(9777..9980)
                     /gene="rpmC"
                     /locus_tag="VI935_00470"
     CDS             complement(9777..9980)
                     /gene="rpmC"
                     /locus_tag="VI935_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013858087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HLE24102.1"
                     /translation="MKPAETRELTGDELKRKERDVKEELFNLEVQLSTRQTTNVSRVK
                     KVKKDLARVLTVMRERELGIRRR"
     gene            complement(9977..10402)
                     /gene="rplP"
                     /locus_tag="VI935_00475"
     CDS             complement(9977..10402)
                     /gene="rplP"
                     /locus_tag="VI935_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HLE24103.1"
                     /translation="MLQPARTKYRKQFKGRTRGIATRGATLAFGEFGLQSLECARLTS
                     QQIEAGRVAVTRHVKRGAKLWVRIFPDKPVTKKPAETRMGKGKGDVAGYVAPIKRGTM
                     LYEISGLAPEVAKEALRLASHKLPVKTRIVIREEGVLAG"
     gene            complement(10582..11235)
                     /gene="rpsC"
                     /locus_tag="VI935_00480"
     CDS             complement(10582..11235)
                     /gene="rpsC"
                     /locus_tag="VI935_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HLE24104.1"
                     /translation="MGQKICPIGLRIGITKSWDSKWYAEKKKYTELLHEDLKVRELIK
                     KGFYQSGISKIELERAASEKVRIVVHAARPGMIIGRRGQEIESLRRDLSNITGKEVYL
                     DIREVKRPEIDAQLASENIALQLERRVAFRRAMKRAMASAMRLGALGIKVMVAGRLGG
                     SEIARKEWYREGRVPLATLRADIDYGFAEAKTTYGVIGIKVWIFKGEVLEKKGRQLT"
     gene            complement(11237..11575)
                     /gene="rplV"
                     /locus_tag="VI935_00485"
     CDS             complement(11237..11575)
                     /gene="rplV"
                     /locus_tag="VI935_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HLE24105.1"
                     /translation="MVSKALLKYTRTSPKKARVVLDLIRGSGVEEAFATLASVKRRPA
                     PIIAKLLKSAVANASEKGHTDTESLYIKEAYASQGPAIKRFRPRAMGRAYPRKRRTSH
                     ITLVLEERGL"
     gene            complement(11596..11886)
                     /gene="rpsS"
                     /locus_tag="VI935_00490"
     CDS             complement(11596..11886)
                     /gene="rpsS"
                     /locus_tag="VI935_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HLE24106.1"
                     /translation="MARSKKKGPYIDLKLLKKVESAKSSGDQRIIKTWSRGSMVIPEM
                     IGLTFAVYNGRMFIPVYITEHMVGHKLGEFAPTRTFRGHGAGDKKAKVEKVQ"
     gene            complement(12034..12876)
                     /gene="rplB"
                     /locus_tag="VI935_00495"
     CDS             complement(12034..12876)
                     /gene="rplB"
                     /locus_tag="VI935_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HLE24107.1"
                     /translation="MGIKKYRPVTKGRRFMTSLDFSEITEDKPFKPLTVPLKNQVGRN
                     SQGRVSSLHRGGGHKRLYRVIDFKRDKFNVPGKVLSIEYDPNRSARIALIGYGDGEKR
                     YMIAPDGLVVGDKVISGENVDVLNGNALPLRNIPVGNFVHNIELKPLGGGKLARAAGA
                     SAQLLAKEGDYAQLRMGSAEIRLIHLNCMATVGRVGNVEHELIVIGKAGRSRYMGKRP
                     HVRGVAMNPVDHPHGGGEGKAPQGNPHPVSPWGWATIGYKTRKNKRTTKFIVKRRRIG
                     YGMD"
     gene            complement(12883..13170)
                     /gene="rplW"
                     /locus_tag="VI935_00500"
     CDS             complement(12883..13170)
                     /gene="rplW"
                     /locus_tag="VI935_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HLE24108.1"
                     /translation="MREPREVVKHPLVTEKSSAIKDTGWYVFAVDGRANKKEIKESVE
                     KLFKVKVDRVRTLVLAGKSVKKFGRISGKRSPLKKAYVKLKEGKIEFFEGV"
BASE COUNT         3425 a         3458 c         2044 g         4305 t
ORIGIN      
        1 aggatttcaa tctgatgccc ggtgataatg tcacctgcct ttacgtcgca ttctcccata
       61 gcactgactg taattaactg aggttcatag gtatgcattt tgagctctat tcccttgaga
      121 ttcaatacaa tctccgtcac atcctcttta acaccaggga tagtagaaaa ttcatgaagc
      181 actccttcta ttttaagcga tgtaattgca ggcccttgta tagaagatag caacactctt
      241 cttaatgcat ttccaaacgt gactccatat ccacgttcaa gcggctcaca tatgaacttc
      301 ccataaacca tacttgaatc cttctcatcc ttttcaagcc tttttggcct tattagttcc
      361 tgccaatttc tctgaaaatc cttcaccttg cctccctccc tttgttagtt ataggttgct
      421 ttttcaaaat taatattaag aataccccat taatgatcag actcttgaat agaattccac
      481 tattagctgc tcttctattg gaaacgtaac atctatcctt tgtggaagcc ttttaactac
      541 tcctttataa ctctctttct caagttcaag ccaatcagga agacctcgtc ttgcaaggat
      601 ttcaagcgac ttattaattc tatcaagctt ttgacttcca tgctttacct caataacgtc
      661 gctcagctta gtttgatatg atggtatatc caatttccta ccgttaataa gaatatgccc
      721 atagcgcact agctcccgtg cctccttcct cgaacctgca aaaccaagac ggtatacaac
      781 gttatcaagc cttctttcca gtaatgaaat tagaagctca ccagtaatac ccttgacacg
      841 ggaagccctt tcaaaatacc ttctgaactg cctttcagta agaccataaa ttcgtctcac
      901 cttttgtttt tcccttaaac gtatcccaaa ctcagaaaac ttaggacgtc ttagaccgtg
      961 ctcaccagga acttggtcag gacgcctctc tactgcgcac ttttctgtgt agcacctatc
     1021 gcctttgagg aatagtttta ttccttccct tctacataac ctgcaaacag ccccagtgta
     1081 ttttgccact tatccgttaa cctccatttt tctatcgcgg atttcgtgat tcgtgattcg
     1141 atacacgagc cacgaacaac gattcacgat gcacaatcta tgatgagtaa ttcatctaat
     1201 tttttacatg caccatgcat cttgtatcat gtatccttca tcctgtatct atacccttct
     1261 tcttccagct ggtctgcaac cattatgagg aattggagtt acatctctaa tcattgttat
     1321 tctaagccct gcagactgaa gagatctgat ggccggttct cttcctggac caggcccttt
     1381 gacataaacg acaaggcttt tcattccatg cgctaccact ttccttgcag catcctcagc
     1441 agcaatctga gcagcaaatg gtgttccttt tcttgtaccc ttgaatccct ttgcaccgct
     1501 gcttgaccaa gcgataacgt ttccttggtt atcacttata gtaattatag tgttattgaa
     1561 tgtagcctga atatgagcca caccctgatc tgcaaacttt ttgctctttt tagccgcctt
     1621 tttcttagcc aatattcacc tccagattaa gaacaagctc caataatcaa atcataaatt
     1681 ccaaattcta agccttgatg ttcttgattt ggaatttggt gtttataatt tgtttgatat
     1741 ttggtgcttg tgatttggaa tttgtagttt catttaaccc ttaagctttt tccgttttgt
     1801 aatagccgtg cctcttggtc ccttcctagt tctggcatta gaacgcgtcc gctgcccacg
     1861 aactggaaga ccaagcttat gtctcgttcc tctatagcag cctatttcga tgaagcgttt
     1921 gatatttgtc tgaacctctt ttcgtaattc accctcaacc gtgtaatccc tttcaattac
     1981 gtccctaatc ttggctatct cctgctcgtt taagtccttt gagcgcctgt ttaggtcaac
     2041 ctccgctttc gcaagaatct tccttgatgt cagacgacca atcccataaa tgaaggtaag
     2101 accaacctca acccttttat tcggtggaat atctacaccc gctattctcg gcatccagtt
     2161 acctcctaac cttgtttttg catatgcttt ttgttattac agattactcg aatggctcca
     2221 cgccttttta tcaccttgca tttatcacaa atcttcttca ctgatgccct tactttcaac
     2281 ttaaactcct tctcttttat ttcttgtaac tattttttca acctgtatgt tattcttcct
     2341 ttagtgaggt cgtagggtga tagctcaacc ttcaccttat cacccggaag aattctgatg
     2401 aaatgagtcc tcatctttcc agaaacataa gcaagtatct ttggaccacc ttcaatctcc
     2461 actctaaaca tagcatttgg aagcgcctca ataactgtac cctcaaactc tattctttcc
     2521 tctttcggca taacctcctc actatctaaa ttcagtcaaa atcttagggc catcctcgag
     2581 tattgcaaca gtatgctcaa agtgagctga aagggtacca tccacagtca cagcagtcca
     2641 accatcaccc aaaaccttga catcaggtct tccaacatta accataggct ctattgctat
     2701 taccatacca ggtttaagtc taatgccctt tccccagtgt ccattctctg gaacaaaatt
     2761 gggtacctga ggatcttcgt gaagcttcct cccaattcca tgtcctacaa aggccctcac
     2821 aactgagaat ccctcattct cgacgcaatt ttgaatagct tgagagatgt caaaaagcct
     2881 attagacgcc cgggccattt tgattccctc atagagagaa ttcctcgtta ctcgaagtag
     2941 gctatcagca agttgagata ccttacctac tggcacagta attgccgaat ccccatagaa
     3001 tccgtcaaga taggcaccaa aatccaggct tacaatatcg ccttctttca atatcttttt
     3061 ttttgatgga attccatgaa caacctcttc gttaactgcg aagcaaacag aggcggggta
     3121 acctcgatag cctttaaaag ctggtatggc ccccttttta aaggtaagct cctctgcaat
     3181 tttatcgagg ccccatgtag aaacaccagg ctttgtttcc tcactaagcc tttctagaac
     3241 ttcgacaact atctttgaag cccttctaat cttttctaat tcctcttcgc tctttaaaaa
     3301 aatcatctaa gcaatacctt ttaatatctt ttcgatcctc tcaaacacat catcaatcgt
     3361 acctagccca tcaacccttt ttaagattcc taccttttta tagaatttct ttaatggctc
     3421 ggtctgcctt cgataaacct gaagtcttgt ccttatcaca tcttcactat cgtcatctct
     3481 ttgataaagc ccacccccac atttgttaca ataccccata tttgttggtg gatcgaaaat
     3541 aatatgatac attgtgccac atttttcgca aacccgccta ccacttatcc tcttaataat
     3601 ctcttcatca tccacctcaa tagagattac actatctatc ctttgtccct ctttactaag
     3661 catcctttcc aaagcctccg cttgctctat tgtccttgga aaaccgtcga gaagaaaccc
     3721 cttttgcgaa tcttgcattg caatcctttc ttttattatc ccaattatta cgttatcagg
     3781 aactaattca cccttatcca tatatgtttt agcaagcttt ccaagttctg tcccttcttt
     3841 cacagaggcc ctcaatatat ctcctgtaga gatatgaagg accttgtaat cttcagaaat
     3901 cttagctgct tgtgtgcctt ttcccgcacc tggtggtccg aaaattacaa gtctcataga
     3961 ttatattcca tacctcctag gccctttcat tccccttttt cttataaatc cctcataact
     4021 gtgtgtaatt agaaaggact caatctgttg tacggtatca agtgttactc ctacaacaat
     4081 gagtagggat gtaccaccga agaagaaggg tatattaaaa ggggctccct gaagaataga
     4141 gggtagcaca cataccgctg caacgtatat cgcaccaata aaggtaatcc ttccaagaat
     4201 cttttcaata tactcagcag ttttctttcc cggtctaatc ccaggtataa aaccgccgtt
     4261 ttttcttaga ttatcagcaa gatcatccgg attataaata actgctgtat aaaagtaggc
     4321 aaaaaatatt attaaacctg catacattgc attataaata aaaccctggc ttaggaataa
     4381 atccgtcata tccctcaaaa ctgggatatt tacaaaacta agtatagttg ctggaaaaat
     4441 cattagcgaa gaggcaaaaa tgggcggtat gactccggct gtattaactt tgagtggaag
     4501 gtgagaagcc tgacccccat agaccttcct tcccactacg cgcctaggat attgaacagg
     4561 tatctttcta taagaccttt caaaataaac tattaagcca acaatgaaag ccataaatgc
     4621 aaaaagaaga agcattccaa atacagtcag ttcaccagtt tctattaacc ttaccgagtc
     4681 ccaaagggca gccggcaccc ttgtaataat tgaagccgca attataagag atataccgtt
     4741 tcctattcca cgctcagaaa tctgctcacc tatccacatt acaaaaaggg tgccagcagt
     4801 tagggtcata acggtagtaa atctaaacat ccatcccgga tctctaacaa cagaggcacc
     4861 agaaccaagc ccaccttcaa gcgtgatagc aagcattagt ccctggataa tacatattag
     4921 tacagtgcca taacgagagt actgattgat cttcctcctt ccggcttctc cctctttctg
     4981 catagcctca agcgtaggga acgccttcac aaggagagac atgatgattg aggatgtaat
     5041 ataaggcatc acgcctagag cgaagatgga agcccgctca agcgcaccac ctgaaaacag
     5101 gtttaatatg tcgaatattg tcccctttgt ctgctcgaaa agctttatta actcttcagg
     5161 atcaatccca ggtgtcggaa caaacacacc taatctataa acaacgagca tcaaggcagc
     5221 aaaaagaagc cttcgattaa gctcaggaat cttagggata gctcctatat tcctactcat
     5281 aaaatctctg cttttcctcc tcgttcttca atctttcttt tagcttcctt actgaaaaca
     5341 tgtgctctaa tagtaagtgc agcattaatt tccccatccc caagtatctt cacaggattt
     5401 ttaccactca caaaaccaag cttcactagc tcttcggggg ttacgacttc accatcttta
     5461 aaattattta agtccttaac attaactatg tcaaaatggg tcttttgaga acttctaaaa
     5521 ccacgcttgg gggtcctcat tttaagagga gtctgtccac cctcaaacca cggggatacc
     5581 ctaccgccag agcgtgatct ctgtccctta tgtccctttc cagaagtctt tccatgacaa
     5641 ccgggacccc tgcctaatct ctttctattc ttcgctgcac cttccggaga actcaatcta
     5701 tttaacattt tataatcacc ctatcacacc aagaaataat aactgtaaaa ttaaacggtc
     5761 tatactcatc ttaatgccga ggttatattg cgctagaatg tattttacac agataataag
     5821 ctaataatta ctctagcttg ttcatattct aaaggccgca tcaaatttat cattcttgat
     5881 agtacttagg aaggtctaaa tcctttgtct cacgtcctct cagtctagca acattctcag
     5941 gtatttttaa tgaagataac cccttaatag ctgctacaac tacattaagg gggtttgttg
     6001 agcctagaac cttagatagt atatcttgaa taccggcaag ctctacaaca gctcgtaccg
     6061 ctccacctgc aataacccca gtaccaggag cagcgggtct tagaataact cgacttgcac
     6121 tttgttcacc aataatatca tgcggaatag tactaccgtg cctaggaacc cttatgagat
     6181 tcttctttgc cttttctata gcctttctta tagcatctgg aacctcgtta gatttaccca
     6241 gagcggttcc aacgattcct ttattgtctc caataacaac tagtacggta aaatgaaacc
     6301 tctttccacc tttggttacc ttagcaaccc ttctaacatg aacaatcttt tccttcagct
     6361 caagcccact tggatcaact ctttcctttg gcaaatctcc ctcctagtag ggacgcaaaa
     6421 tttttcgtct ctagctaaaa ttcaagccct gcttctcttg ccccgtctgc gagggctttt
     6481 acccttccat ggtaaagata accaccccga tcaaaacaca ccttctttat accccttgat
     6541 aaagccgtct cccctatata ctttccaact aattttgcag tttcaacctt attctttttc
     6601 ccttcattat taagcgtcgt tctaatctta ggtgtaagtg ttgatgctgc taagagtgta
     6661 tgtcctttga tgtcatcaat tatttctgaa taaatatgtt tagaggactt aaagaccgaa
     6721 agcctaggtg tcgaagtacc ccttagcttt cttcttactc ttctgtgtct tatgtcccgt
     6781 ctttcctttc tagtcttctt tcttatcatt tctttgcgcc tgccttacca ggtttgagtt
     6841 taagtatttc atccgagtac cttattccct tacctttgta tgagtctgga agcctaagcc
     6901 ttttaatcct tgctgctgtt tcaccaacaa cctctttatc aattccttga attatcatcc
     6961 ttgtacctct ttcttcaatc ctcactgcta acccttcggg aagagaaaat tcgaccggtt
     7021 ttgaatagcc taagctgaac tttaggttac ttttccccaa aagctcggct ttgtaaccaa
     7081 cacctacaat atcaagagac tttgaaaatc cttcagtaac accaaaaatc atatttgata
     7141 atagagcacg tgttagacca tgaagtgctc taagctcttt attgtcgctt tttctctcga
     7201 cactaacctg actaacatca gccttaacct caatcccaaa aggaatatgt cttgcaagct
     7261 tcccttttgg ccccaaaacc tctactaatc catctttgat attaaccttt accccacttg
     7321 gtataaaaat cgatcttctt cctattctcg acatgatttc ttcctcacca gattgtacat
     7381 ataacctctc ccccgattcc agagcggcgt gcccgaattc ccgtcataat cccttttggg
     7441 gttgaaagga tataaattcc tagccctccc cttatccgag gtatttcatt cttgttaaca
     7501 taaaccctgt gccctggttt actcacccta cgaatctccg ttatcattga ttccttttga
     7561 ggtgtatatt tgagtgctat cttgattatc ttctttattc catcctcgag caccttgaaa
     7621 tcactaatat atccttcctc tttaagaacc ctggcgatgt caaacttaat cctcgatgca
     7681 ggaacatcta caaagccaaa acgagccttt agtgcatttc tgatccttgt aagcatatca
     7741 gctattggat ctgtcattac gattacccct ttttgaataa ttatgcaaga tgcatgactc
     7801 atgatgcatg aaatttagat tcatcaaatc ctgtatcctg tatcctgtta ccaactagcc
     7861 ttcttaaccc ctggcacctt cccaaaacta gccgcttgtc ggaagcataa cctacacata
     7921 ttaaatatcc ttaaatatgc ccttggtcgc ccacagagag ggcagcgatt tctatgtctc
     7981 accctaaact tgggtttgct cctcaatttc tccatcattg actttgtcgc catatgcccc
     8041 tcaataaaat gatgcaagat tcatgataca tgatgcacga ttttatccca tttattcatc
     8101 ttgtatcctg catcttgcat cctgtttttt gctcctcatc ttcattcgtg tcggaataac
     8161 ctattgctat acacgtttct gcgtctcaat agctacccct gttaacgctc cctaaatggc
     8221 attcccatat cctttaatag cccttttgat tcttcattag tttttgctgt ggtgacaatg
     8281 gttatattta ctccctttac cttttcaacc ttgccatact caatctcagg aaatattgta
     8341 tgttccctga gtcctagtgt gaaattaccg ttaccatcaa aggcctgagg agaaactcca
     8401 cgaaaatctc taacacgggg aagggcaaga ttaaccaatc tatcaaaaaa ctcatacatc
     8461 ctttcaccac gtaatgtaac catgcaaccg atagtcgcac cttctcttag cttaaaccct
     8521 gcgattgatt ttcttgctct cgttactact ggcttctgtc cagtaataat ggaaatttcc
     8581 tgaaccgcat catctatgat tttattttct tttccgccat cgctgagggc accgagtccc
     8641 atattaatca caatcttatc tattcgtgga atttccatta agtttttgta tccaaaccgt
     8701 tccttgagca gtttagtaac cttatctttg taatattgct ttagtctcgg aaccatcagc
     8761 tcttaatctc ctcgtcacat cttctgcagt aacgtatctt ttctccgctc gagagccttt
     8821 ttcgtcctat ccttacagga cggctacact ttgaacagta gatcatgagg tttgatatgt
     8881 gaattccggc ttccttctca attatgcctc caccaggatt cttttgtgaa ggacgagcat
     8941 ggcgttttac catatttagt ttttccacaa ccgcttttct cttatccctt aatattctta
     9001 gaaccttgcc tgtttttccc ttttccttac cagaaattac ataaacggta tcaccgcttt
     9061 tgatatgaac ctccttccct tgagtcactt ccatgagtta tacaacctcc ggtgcaagtg
     9121 atattatttt cataaatcct ttagaacgca attcccttgc caccggtcca aaaacacgag
     9181 tgccaatcgg ttcattctcc ttagttataa gcacggcagc attatcatca aaccttacat
     9241 atgagccatc tgagcgcctt tgctccttcc gagtacgtac gattacagcc ctatgtacat
     9301 cacccttttc aaccttagag ttgggtatgg cctcctttac tgaaactact attacatcac
     9361 caagccctgc atattttcta ttagacccac ccataaccct tatacagaaa agtcttttcg
     9421 caccggtatt gtctgcagcc tgaagaatag tagttggttg aatcattctc cctttcctcc
     9481 ctccccaaga tcctctttgg gtagttcggc ttcgagtagc gttgcacgct caaggatttt
     9541 actcactctc catctctttg tacggctgag aggacttgtt tcgatgataa aaactttatt
     9601 gccgattcta caatcgtttt tttcatcgtg agctttgaac ctcgatattc ttttaacatg
     9661 ctttccatac ttcggatggt gaacaatctg agttacatca acgacaacag tcttattcat
     9721 cttgttactt gtaactacac ccactcttgt tttgacttta cctctactca ttctgactat
     9781 cttctcctta tgccaagctc cctctccctc ataactgtaa gcactcgtgc caaatccttt
     9841 ttcaccttct ttaccctcga cacattagtt gtttgacgtg ttgaaagctg aacctctagg
     9901 ttaaaaagct cctcctttac atccctttct tttctcttta attcatcacc agtaagctct
     9961 cttgtttcag caggcttcat ccagcaagca caccctcttc gcgaataact atgcgagttt
    10021 ttacaggtag tttatgtgaa gcaagcctaa gcgcctcttt agcgacttcc ggtgcaagac
    10081 cgcttatttc gtagagcatc gtccctcttt ttattggagc aacataacca gcaacatcac
    10141 cctttccctt tcccatcctt gtctcagcag gcttcttcgt cacgggtttg tcagggaata
    10201 tccttaccca gagcttcgct ccacgcttga catggcgtgt taccgcaaca cgtcctgctt
    10261 cgatctgctg agaagtcaac ctggcgcatt cgagcgattg aagcccaaat tcgccaaagg
    10321 caagcgttgc gccccttgtt gcaatccctc ttgtccttcc cttaaattgc tttctatatt
    10381 ttgttctagc cggttgaagc atctttcttc cttaatctaa gtgtaatact tagccacaaa
    10441 gactctaaga cacgaagata gaattcagag gtcaggagtc agaattacct tttttcttcc
    10501 ggattccggc ttctgactgc tgacttcttc tttaaaaccc atatttctca tttgtgtctt
    10561 ggtgccttag tggctgaata attaggttaa ttgtcgtcct ttcttctcca acacctcgcc
    10621 tttaaatatc cataccttaa tacctatcac accgtatgtt gtctttgctt ctgcaaatcc
    10681 atagtcaata tccgcacgaa gcgttgcaag tggaacccta ccctcgcggt accattcctt
    10741 ccttgctatt tctgatcccc ctaaccttcc tgcaaccatt accttaatcc cgagtgcacc
    10801 gagtctcata gcagaggcca tcgccctttt catagctcgc ctaaaagcta cacgcctttc
    10861 aagctgtaac gctatgtttt ccgaagcgag ctgtgcatct atctcagggc gtttgacttc
    10921 acgaatatcc aaataaactt ccttacctgt aatattactc aaatctcgtc taagagattc
    10981 gatctcttgt ccccttcgtc ctattatcat tccaggtcga gcggcatgaa ctactattct
    11041 taccttttca gaagccgccc tttctagctc gatttttgag atcccagatt gataaaaccc
    11101 ctttttaatg agttctctaa ccttcaaatc ttcatgaagg agctctgtat acttcttctt
    11161 ctccgcatac cactttgaat cccaactttt agttattccg attctaagtc caatcgggca
    11221 gatcttctgt cccaagttat aacccccttt cttcaagcac aagcgttata tggctagttc
    11281 ttctttttct gggataagcc cttcccattg ctcttggtct aaatctttta atcgcaggcc
    11341 cttgagaggc atatgcctcc tttatataaa gagattcggt atcagtatga cctttctcac
    11401 tagcattggc tacagcggat ttaagaagtt tagcaataat tggcgcaggc ctccttttta
    11461 ctgacgcaag ggttgcaaag gcttcttcaa cgccactccc tcttattaaa tcgagtacaa
    11521 cccgcgcctt ctttggtgat gttctagtat atttaagcaa tgcttttgat accatttttt
    11581 tattacccta attatttact gaaccttttc aacctttgcc tttttatccc ctgctccatg
    11641 gcccctaaag gtacgagttg gagcaaactc accaagctta tgtcctacca tatgttctgt
    11701 tatatataca ggtatgaaca ttctcccatt atatacagca aaagtaagcc ctatcatctc
    11761 aggtataacc atagagcccc ttgaccaagt ttttataatg cgttgatccc ctgaggactt
    11821 tgctgactca accttcttta gtagttttaa atctatgtaa ggccccttct ttttcgatct
    11881 agccattttc actccttaag ttaaatgatg cacgatgcat gattcatgat acacgaagat
    11941 tttacatgca tcctgtatca tgcatcttgc atccggcata cagcctttcc attcgctatc
    12001 atttgtgtca attcgggatt tatagttaac tctttaatcc attccataac caattcttct
    12061 tcgctttact ataaatttcg ttgtcctctt gttttttctt gttttatatc cgattgtagc
    12121 ccatccccaa ggtgacaccg ggtgaggatt tccttgtgga gcctttccct ctccaccacc
    12181 gtgtggatgg tctacgggat tcatcgcgac acctctcaca tggggacgct ttcccatata
    12241 cctacttctt ccagcctttc caatcactat taattcatgc tccacgttgc caacccttcc
    12301 aacggtcgcc atacaattta gatgaattaa acgaatctca gcagagccca tcctaagctg
    12361 tgcatagtcg ccctccttag ctaagagttg tgccgatgcc ccagcggctc gtgcgagctt
    12421 ccctccccct aagggtttta attctatatt gtgaacgaaa tttccaactg gaatattcct
    12481 taaaggaagt gcgtttccat taagaacgtc cacattctct cctgagatta ccttatcgcc
    12541 aaccaccaac ccatctgggg ctatcatgta tctcttctct ccatccccat aacctataag
    12601 cgcaatcctt gctgaccgat tagggtcata ttctattgat aacactttac cgggcacatt
    12661 gaacttatcc cttttgaaat caataactct atataagcgc ttgtgaccgc ccccacggtg
    12721 gaggcttgaa acccttccct gagagtttct ccctacttga ttcttaagag gcaccgttag
    12781 aggtttaaaa ggtttatcct cagtaatctc tgaaaagtct aggcttgtca tgaaccttct
    12841 acctttagta actggacgat atttcttaat ccccataata acttatactc cttcgaagaa
    12901 ctcaatcttg ccttctttta gtttcacata tgccttctta agtggagacc tcttaccaga
    12961 tatgcgaccg aatttcttca cactctttcc agccaaaaca agcgttctta ctctatccac
    13021 ctttacttta aagagcttct ctacagattc ctttatctcc tttttattag cccttccatc
    13081 aaccgcaaat acataccatc cagtatcctt tattgctgat gatttttctg taacaagagg
    13141 atgcttaacg acttctcttg gttctctcaa ttctttagca cctcctgaac ccgactaagc
    13201 gaatcctctg tcataacaag tgagttaaac ct
//
LOCUS       DATDRL010000012         9966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_522219, whole genome shotgun sequence.
ACCESSION   DATDRL010000012 DATDRL010000000
VERSION     DATDRL010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9966
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522219"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VI935_00505"
     CDS             complement(<1..486)
                     /locus_tag="VI935_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24109.1"
                     /translation="MDFRNRLFASAAKVKSEDREQTEFFAAEFLERLILFEEYILESI
                     RLKEIPHLVQLFGNSPVLELLKSGKLKIYCDAVTMGSTGQAGGALESRKIKGNLPLSS
                     YCFDMIQTAYYKDYISKFLQQIHDINGISHKEAKKLKLAVVEKLVSFPELALSQIQTQ
                     TI"
     gene            complement(553..867)
                     /locus_tag="VI935_00510"
     CDS             complement(553..867)
                     /locus_tag="VI935_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24110.1"
                     /translation="MSDSKIETLRALVEKNPNNPLGRYGLANEYIKLEMYEEAINEIN
                     AYLKLKDDEGAVYRILGEALLKLGRKGEAKEAYRNGIEAAERHGHPGMAAEFEETLEL
                     ID"
     gene            complement(1009..2184)
                     /locus_tag="VI935_00515"
     CDS             complement(1009..2184)
                     /locus_tag="VI935_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014685137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HLE24111.1"
                     /translation="MGTFKITLKQIGISLKILNDCLEDEKYSIHSLITIILAVLVFIS
                     CTKLAYSSVDEIRLPNGFKIEEFASNLGSPRFMAFSPDDVLFVTIIGKGTVVALPDKD
                     KNGKTDKVITFVKGLNHPHGIAFYKGYLYIGETNQIVRFKYQGFDSTPGKKEIIVPNL
                     PARGHFTRTVGFGPDEKMYVSIGSSCNVCEEKDERRAAILQFNPDGGEERVFAKGLRN
                     SVGITWNPETKELWATDNGRDWLGDNLPPEEINIVKFEKHYGWPYCYGDRVPDPDFDT
                     PDFCKKTTPPVFEMQAHSAPLGLTFYMGKLFPEEYQGDLIVAFHGSWNRSMPTGYKVV
                     RIKMKNGKPQKIEDFATGWLKGQRAWGRPVDLIVGPDGSLYVSDDKGGFIYRITVTK"
     gene            complement(2364..2534)
                     /locus_tag="VI935_00520"
     CDS             complement(2364..2534)
                     /locus_tag="VI935_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLE24112.1"
                     /translation="MKEVSYIVYQEGEYYVAQCLNVDVSSFGENIDEAITNLIEAVEL
                     YLEGEDLNIPGI"
     gene            complement(2647..4179)
                     /locus_tag="VI935_00525"
     CDS             complement(2647..4179)
                     /locus_tag="VI935_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HLE24113.1"
                     /translation="MNNRITEEAWNYHNKTKHPGMPTHYLDWANQPIPFKIYTTLDTI
                     EIPADLPPSNVPALTAISNNVPITSDKCVPDLKTLGRLLYLSAGITKKVKYPGGEIYF
                     RAAACTGALYHIDLYIVTRDIEGLDAGVYHFGPHDFSLRILRKGNYRGVIMSASVEEA
                     AITKAPITIITTSTFWRNSWKYQSRAYRHTFWDTGTILANLLAAASAYKIPAKIITGF
                     VDSTINKLLGLDTNREVAVSLVPLGNTDEPTQAKQGEIKTINYETMPLSKKEVDYPLI
                     RSIHQASSLTSEEKVRDWRTATTKSTQPEINGKLFSLDNQDKEISQISKTLEETILKR
                     GSTRQFARTSITFKQLSNILYHSTSGIRADFLNPFGSTLNDMYLIVNDVEGIPKGAYF
                     YRRNDNALELLKEGDFRDDAGYLGLGQDIPGDASVDVFFLTDLNQVLEKFGNRGYRVA
                     KLEAGIIGGKLYLGAYSQNLGASGLTFFDDDVIRFFSPHAEGKSVMFLVALGKSIKRK
                     SG"
     gene            complement(4308..4676)
                     /locus_tag="VI935_00530"
     CDS             complement(4308..4676)
                     /locus_tag="VI935_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24114.1"
                     /translation="MVSYGAYKLIHVLGIMFLLLSLGAYLILSMNKSEKGKKLAAITH
                     GISVLIILVAGFGLLARLGYLSVETWPIWVWLKVIIWLILALIIILIKRMPELIPALW
                     FLIPILGGLAAYFAIYKVSF"
     gene            4885..5922
                     /locus_tag="VI935_00535"
     CDS             4885..5922
                     /locus_tag="VI935_00535"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLE24115.1"
                     /translation="MIAILTGGTGFIGKNLVSSLIARSYKVRCLVRSTSRVEGLSSLG
                     VELRCGDLSDLRTLKEITKGGEVVYHLAAVVSDWGSKEEFYRVNVEGTENLLKASVEN
                     GVKRFVFLSASSVLWSYNLWKINDLDNVDESYPYPVSYKDLYNETKAEAEKMVSEYFN
                     KTGLETVILRSSAVWGPGDTVILPRVVRAAKKGILTFVGKGDKWVSPCYVENLAEALI
                     LAGERENIAGNIYFINDGIKLDYRYFISLLLKTVGVDWSPGVSVPYSLAYGLASMIGL
                     GYKIVRFKKPPTLTRFAVASLAGSRTYSIEKARSQLGYHPNVTLEEGLRHLEEWVNSI
                     GGTEGLLGIRR"
     gene            complement(5905..6606)
                     /gene="lipB"
                     /locus_tag="VI935_00540"
     CDS             complement(5905..6606)
                     /gene="lipB"
                     /locus_tag="VI935_00540"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HLE24116.1"
                     /translation="MKLKKMDGFKVYRLGLVDYQDALKLQFSILEKRMKEEIEDVLLL
                     LQHPPTFTIGRSGKTENLLSNIEELKKRGIHFEEISRGGDITYHGPGQLVGYPIVNLN
                     KFRRDIHRYLRNVEEMIIYALSDFHIKAGRINALTGVWVNEEKIASIGVGVRRWITYH
                     GFALNVNTDLSYFDMIIPCGIPGAKITSMQKLLGEREDVNMMKVESSIINAFSSVFNR
                     ILISEITSDDLSSYT"
     gene            7098..9791
                     /gene="aceE"
                     /locus_tag="VI935_00545"
     CDS             7098..9791
                     /gene="aceE"
                     /locus_tag="VI935_00545"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HLE24117.1"
                     /translation="MVSVIDKEIKNKELSELEAIETHEWLESLDHILQRGGPERIRRL
                     LRQLQIHAQEAGVKLPFTANTPYINTIPAEEQPPFPGSREIERRIKSIIRWNAMAMVV
                     RANRFEDGVGGHISTYASAATLYEIGFNYFFRAKTEDHEGDIIYFQGHASPGIYARAF
                     LEGRLSVEQLANFRRELQHGGGLSSYPHPWLMPNFWEFPTVSMGLSPIMAIYQARFNR
                     YLEDRGLKKPSDAKVWAFVGDGEVDEPESLGAITLASRENLDNLIFVINCNLQRLDGP
                     VRGNGKIIQELEAIFRGAGWNVIKVIWGDDWDPLLAKDHDGILVKVMEENVDGQYQKY
                     IVETGKYIREHFFGADPRLLDMVKHLSDEQLQKLNRGGHDPEKVYAAYKAAVEHKGSS
                     TVILAKTIKGYGLGESGEGKNITHQQKKLNEEELREFRSRFGIPISDEDVVKAPFYKP
                     HDESPEMKYLHARRRELGGYIPSRTVKCEPLKTPSDELFEEFYKGSDGRDVSTTMAYV
                     RVLAKLLRDKEIGKLIVPIIPDEARTFGMEVLFRRFGIYSHVGQLYEPVDMDTLLYYK
                     EAKDGQILEEGITEAGSMSSFMAAGTAYATHGINTIPFFTFYSMFGFQRIGDLIWAAG
                     DMRCRGFLVGGTSGGTTLAGEGLQHQDGHSHLLAYPVPNLKAYDPAFAYEIAVIIRDG
                     IHHMYEEQQNIFYYLTVTNEPYVQPPMPDGVKEGILKGLYKFKASDNKNAKLHAHLFG
                     SGAILNEALKAQKILEEKYEIATDVWSVTSYKELYREGHEAERWNILHPSELPKIPYV
                     TKCLQDASGVFVAASDYVKAIPDSITRWLPKPIVSLGTDGFGRSDSRRALRDFFEVDS
                     RFITLATLTALAREKEIKTDVVQQAIKDLEINPAKANPMIS"
BASE COUNT         2924 a         1915 c         2075 g         3052 t
ORIGIN      
        1 tattgtctgc gtttggatct ggcttaaagc taattctgga aaagacacta atttttcaac
       61 aactgcgagc tttagttttt tagcctcctt atgagaaatg ccatttatat catgaatttg
      121 ttggagaaat ttactaatat aatctttata atatgcagtt tgaatcatat caaaacagta
      181 agaactaagt ggaagattac ccttaatctt tcgtgactct aatgcacctc ctgcctgacc
      241 agttgacccc atagttactg catcacaata gattttcaat tttccagatt ttaatagctc
      301 caggactgga gaatttccaa ataactgtac taagtgtggg atttctttaa gtcgaatgct
      361 ttcaaggata tattcttcaa aaagaattaa ccgttcgaga aactctgcag caaaaaattc
      421 agtttgctct cggtcctcag atttaacttt ggcggctgat gcaaacaaac gatttctgaa
      481 atccataact aaatatccat tgtcaatgcc tttatttttc taacttttct atgctaaaca
      541 gaaaaatatc ttctaatcaa taagctcaag tgtttcttca aattctgctg ccattcctgg
      601 atgaccgtga cgttctgcag cttcaatccc atttcgatac gcttcttttg cttctccctt
      661 tcttcctagt ttaaggagtg cttctccaag tattctgtag acggcaccct catcatcttt
      721 gagttttaag tacgcattaa tttcattaat tgcttcttcg tacatctcca attttatata
      781 ctcattcgca agtccgtacc ttccaagagg attgttagga tttttttcaa caagggctct
      841 cagggtttca attttagaat cactcatcga agtctccgat tttgaggttt tttatattat
      901 atattaacta aattaggagc acattgagat gagtagactt aatgtaaatt tcgtccattc
      961 tatagataga aaaattagta cattaagttt ctgataaaga atgagggttt atttagtaac
     1021 tgttattcta taaatgaatc cacctttatc atctgagaca taaaggcttc catccggtcc
     1081 tactattaaa tcaacaggtc ttccccaagc cctttggccc tttagccatc ctgttgcgaa
     1141 gtcttctatc ttttgaggtt taccgttttt catttttatt cgaacgactt tgtatccggt
     1201 aggcattgag cggttccatg agccatgaaa ggcaacaatt aaatctcctt ggtattcttc
     1261 tggaaaaagt tttcccatat aaaaggtaag tccaagtggt gctgaatgtg cctgcatttc
     1321 aaatacgggt ggagttgttt ttttacaaaa atcaggggta tcaaaatctg gatcaggaac
     1381 tctatcccca tagcaatagg gccatccgta gtgtttttca aatttaacaa tatttatttc
     1441 ctctgggggt aaattatctc caagccagtc cctaccgtta tcagtagccc ataactcctt
     1501 tgtctcaggg ttccatgtaa taccaacaga gttccttaac cctttggcga agaccctttc
     1561 ttcaccacca tctgggttaa actgaagaat tgctgcgcgc ctttcgtcct tttcttcaca
     1621 gacattacat gaagagccga tagaaacgta catcttttca tccgggccga aaccgacggt
     1681 tcttgtaaag tgaccgcgag cgggtaggtt aggaacaatt atctccttct tgccaggagt
     1741 tgagtcgaag ccttgatatt taaaacgtac aatctggttg gtttctccta tatagagata
     1801 gcctttgtaa aaggctatgc cgtgaggatg atttaatcct ttaacgaatg ttattacttt
     1861 atctgttttc ccgtttttat ctttatcagg aagtgctaca actgttcctt taccaatgat
     1921 agttacaaat aacacgtcat ctgggctgaa ggccatgaac cttgggctcc cgagatttga
     1981 ggcaaattct tctattttaa atccattggg gagacgtatt tcatcaactg aggaataagc
     2041 gagtttagta caagaaataa aaactaagac tgctaatatt atggtaataa gcgaatgtat
     2101 tgaatacttt tcatcttcta aacaatcgtt taaaatcttt aagcttattc caatctgttt
     2161 tagtgtaatt ttaaaggttc ccattggtat ttctttttat tcataggaag gatggctatt
     2221 ttaccacttt agtttttgaa tttgccatga gaacttctta gtgaagtaag tttgaaatta
     2281 agtcgagtta gcactcgttc tatttccttt ggagagtaca acttagcctc tgactcaatc
     2341 ttttcctact atactataac ttttcatata ccaggtatgt ttaggtcttc accttcgaga
     2401 tatagttcca cagcttctat aagattagtt atagcctcat caatattttc accaaatgag
     2461 gatacatcaa catttaaaca ctgcgctacg taatactctc cttcttgata aacgatataa
     2521 cttacctctt tcatgattct ttaacccgaa caatatttaa acttatttta tatacaaata
     2581 aatactatac ggatctgaga tttcagattt tcccaacatt ttatctataa tcagatgcta
     2641 gtttgtctat ccagacttcc tttttataga tttaccgagc gcgacgagga acatgacgct
     2701 cttgccctcc gcatgcggtg agaaaaaccg aattacatca tcatcgaaaa aggtgagtcc
     2761 actagcaccg agattctgac tataggcccc gaggtagagt tttcctccga taatgcctgc
     2821 ttcaagcttg gctactcggt agcctcggtt gccaaatttt tcaagcactt ggtttaagtc
     2881 agtcagaaag aaaacatcca cgcttgcatc ccccggtatg tcctggccta gtcctagata
     2941 cccagcatca tctctaaaat caccttcctt cagcaactca agggcgttgt cattgcgacg
     3001 gtaaaaatag gctccttttg ggattccttc aacgtcatta acaattagat acatgtcgtt
     3061 aagggttgag ccaaatggat tgaggaagtc tgcacggatt ccgctagtag aatgatatag
     3121 tatgttagat agttgtttaa atgtaattga ggtccgagcg aattgacgtg tagacccacg
     3181 ttttagaatt gtttcttcta atgtttttga aatctgacta atctctttat cctgattatc
     3241 gagtgaaaag agcttgccgt taatctcagg ctgcgtggat ttagtggttg cagttctcca
     3301 atctctcacc ttttcttcac tcgtaagtga agaagcctgg tgtatagatc taatgagcgg
     3361 gtaatcaacc tcctttttag ataacggcat ggtttcataa tttattgtct taatttctcc
     3421 ctgttttgcc tgagtgggtt catctgtatt tcctaatggt acaagagaaa ccgctacttc
     3481 acgatttgta tcaaggccaa gtagcttatt tattgttgaa tcaacaaagc ctgtgataat
     3541 ctttgcagga atcttatatg cagaagccgc agctaataaa ttagccagta tcgtgccggt
     3601 gtcccaaaag gtatgcctgt atgcgcggga ctgatatttc caggaatttc gccaaaatgt
     3661 gcttgttgta ataattgtga taggtgcttt ggttattgcc gcctcttcca cagaggcact
     3721 catgataacg cctcgataat tccctttacg aagtattctt aatgaaaaat catgcggtcc
     3781 gaaatgataa acaccagcgt caagcccttc aatgtcccta gttacgatat acaaatcaat
     3841 atgatatagg gctccagtac atgcagcagc gcggaaataa atctcacctc ctggatattt
     3901 aaccttcttt gtgatgccag ccgataggta aagcaagcgt ccaagggttt ttaaatcggg
     3961 tacacattta tcacttgtaa ttgggacatt gtttgatatt gcagttagag ctggaacgtt
     4021 cgaagggggt aggtcggcag gaatctcaat tgtatcgagt gttgtataaa tcttaaaggg
     4081 aataggctgg ttcgcccaat ctaagtaatg agttggcatt cctgggtgtt tggttttatt
     4141 atgataattc caggcttctt ctgtaatacg gttgttcatt atgctttacc ttatgttacg
     4201 aatttcaact ggtaattatt gaaccagagg acacagacag aagatgaaat aaaaagttta
     4261 ttttttattc tttctctatc tgctctgtgc tctctgtggc tattatatca aaagcttacc
     4321 ttataaatcg caaagtacgc tgctagccca cctagaattg gaatcaaaaa ccataaagct
     4381 ggtattagct caggcattct ttttatgagt ataataatta gggcgagtat taaccaaata
     4441 ataaccttaa gccataccca aataggccag gtttcaacac taaggtaacc aagtcgagca
     4501 agtaggccaa agcctgcaac aaggataata agaaccgata tgccatgggt tattgctgca
     4561 agtttcttac ccttttccga tttattcata gaaagaatca aataggctcc taatgaaagt
     4621 agcaagaaca tgattccaag gacgtgtatt aatttgtatg caccgtatga aaccatttca
     4681 cctccgtaaa attatatgct ttactatgct caatcataga tatttaatca actgcttatt
     4741 tacatagact gctaaaataa tgatactaac taattctctt tttgctattc tcgcctttta
     4801 agtgtcatga cggtgccaag attttaatga aggcaataac ttttgtatgg tgaaatttta
     4861 tcatgaagga ttgagattaa aagaatgata gcgatattaa cgggagggac gggttttatt
     4921 gggaaaaatc ttgtgagcag cttgattgct aggagttaca aggtgagatg tttggttaga
     4981 agtacaagca gggtagaagg actaagtagt ttaggtgttg aacttcgctg cggagacctc
     5041 tcagatctta gaacattaaa ggagattaca aaaggtggcg aagtagtcta tcatttagcg
     5101 gcagttgtct cagactgggg atcaaaagag gaattttaca gggttaatgt agaaggaaca
     5161 gaaaacttac taaaagcatc ggtagagaat ggtgtgaaga ggtttgtatt tttaagtgca
     5221 tcaagcgtat tatggagcta caatctttgg aaaataaatg atcttgataa tgttgacgag
     5281 agctatcctt atccagttag ttataaagat ctatacaatg aaacaaaagc agaggcggaa
     5341 aagatggtaa gtgaatattt caataagaca ggattagaaa cggttatttt aaggtcctca
     5401 gcggtgtggg gtcctggaga tacggtgatc cttccaaggg tagtgagggc tgcaaaaaag
     5461 gggattctta cctttgtagg aaagggtgat aaatgggtct caccatgcta tgtagaaaac
     5521 ctggctgaag cactcatact cgcaggcgag agggagaata tagctggaaa tatttatttt
     5581 ataaacgacg gtataaagct agattacagg tattttatat ctttacttct taaaacagtc
     5641 ggcgtagatt ggtcacctgg agtatcagtt ccttattctt tggcctatgg gttagcatca
     5701 atgattgggc ttgggtataa aatagtaaga tttaaaaagc ctccaacgtt aacaaggttt
     5761 gcggttgcat cccttgcggg aagcaggact tatagtattg agaaagcgag gagccaatta
     5821 ggataccatc caaatgtaac tcttgaagaa ggactgaggc atcttgaaga gtgggttaat
     5881 agtataggtg ggacagaagg tttgctaggt atacgaagat aggtcatctg aggttatctc
     5941 actgattagg attctattaa acacgcttga aaatgcatta atgatgcttg attctacctt
     6001 catcatatta acatcctctc tttcacccaa gagtttttgc atagacgtta tttttgctcc
     6061 tgggatgcca catggaatta tcatatcgaa ataagataga tcggtattta cattgagagc
     6121 gaatccgtga tatgtaatcc atctcctaac tccaacacct attgatgcaa tcttttcttc
     6181 gtttacccaa actccagtaa gggcgtttat tcttccagcc ttaatatgaa aatcgctcag
     6241 agcgtaaata atcatttcct cgacgtttct aagataccgg tgaatatcac gtctaaattt
     6301 atttaaatta acaatcgggt atccaaccaa ctgtccagga ccgtggtagg ttatatctcc
     6361 acctctactt atttcctcaa agtggatacc tctttttttt agttcttcta tgtttgaaag
     6421 aagattttcg gtcttaccac tccttccgat tgtaaaggtt gggggatgct ggagaagaag
     6481 gagtacatcc tctatttctt ctttcatcct cttttcaagt atggaaaatt ggagctttag
     6541 tgcatcttga taatcaacta accctaatct atatacctta aacccatcca tcttttttaa
     6601 tttcaatttt tagctaaaga tgaatacgaa cttctacaaa ttaataataa atgactgttt
     6661 tcattattaa aaggcaaaaa tcgagaaata aaagttactt atttcttacg agtgtgtttt
     6721 tgtaatttca tccgattctt gtatagaatc taatcaaagc atatgcaaag ggaacgatac
     6781 aattttgagg gattatggtt aaattctatc cggagtatgt caaatttata aacactactc
     6841 ataaaattca ttgtacattt atacgttaaa aataactggc acgttttatt gtgttgctgt
     6901 gtttaacttt tcacgtagtc attagtttag gtgagagtta gaaacgataa aattggaaaa
     6961 cacaaataca ttggattaaa ttaaattggc gttgtattat ttgttaagta ttcatcaaaa
     7021 ttgaaatgta tatatagttt ctactaatgt gctattgggt taatagcctt gccaatacct
     7081 ttaaggggag ggataacatg gttagtgtaa tagataaaga aattaaaaat aaagagcttt
     7141 ccgaactcga agccatagaa actcacgaat ggcttgagtc cttggaccat atattgcagc
     7201 gtggaggacc tgagcgaatc agacgccttc tcagacagct ccaaatccac gcccaggaag
     7261 ccggagtcaa attgccgttc acggcaaata caccttacat taacactatt ccggctgaag
     7321 aacagcctcc atttcccggc agcagagaga ttgagcggcg cattaaaagt atcattcgct
     7381 ggaacgccat ggctatggtg gtacgtgcaa atcgttttga ggatggggtt ggagggcaca
     7441 tctcgactta tgcctcggcg gcgacattgt atgaaattgg ctttaattat tttttccgtg
     7501 cgaagacaga agatcatgag ggtgatatta tctattttca gggtcatgcc tctccaggaa
     7561 tttatgcgcg ggccttctta gaaggtcgtc tgtccgtaga acagttggcg aacttcaggc
     7621 gtgagcttca gcatggaggg ggactttcat cttacccaca tccatggctt atgcctaact
     7681 tctgggaatt cccaacggtg tcaatggggc taagccccat catggcaatc taccaggccc
     7741 gatttaaccg ctatctcgaa gatcgagggt tgaagaagcc ctctgatgca aaagtgtggg
     7801 cgtttgtagg ggatggagag gttgatgagc ctgaatcact cggagctatc accctcgcat
     7861 caagagagaa tcttgataac ttgatttttg tgattaattg caatttgcaa agactggacg
     7921 gacctgtgcg tggcaatggt aaaatcatcc aagaactcga agccattttc cgtggcgcgg
     7981 gatggaatgt aataaaggtc atatgggggg atgactggga tccacttctt gccaaggatc
     8041 acgatggcat tctggtcaaa gtgatggagg aaaatgttga tggacagtat cagaaataca
     8101 tcgtcgaaac tggtaaatat atacgtgagc acttctttgg tgccgatcca agactattag
     8161 atatggtgaa acacttgtct gatgagcaac tccaaaagct aaatcgtggc gggcacgatc
     8221 cagaaaaggt ttatgcggcc tataaggcag cggtggaaca taaaggatcg tcaactgtga
     8281 tactggctaa aacgatcaaa ggctatggac ttggtgaaag cggcgaaggc aaaaatataa
     8341 cccatcagca gaaaaaattg aatgaggaag agttgcgtga atttcgtagt cgattcggca
     8401 ttcctatttc cgatgaagat gttgtgaaag ctccttttta caaacctcat gatgagagcc
     8461 ctgaaatgaa atatctccac gcacgtcgac gagaactcgg gggctatatt ccaagccgta
     8521 cggttaagtg cgagcctctc aagacaccat cggatgaatt gtttgaggag ttctacaagg
     8581 gttcagacgg tcgagatgtg tccacaacga tggcctatgt tcgggtattg gccaagttac
     8641 tccgagacaa ggaaatcgga aaactgattg ttcctattat cccggatgag gcgcgaacgt
     8701 ttggtatgga ggtgttgttt cggcggtttg gtatttactc tcacgttggt caactctacg
     8761 agccagttga tatggacacc ctcctctatt acaaggaagc caaggacgga cagatcctag
     8821 aagaggggat cacggaagct ggttcgatgt cctcttttat ggctgccgga acagcatatg
     8881 ccacccacgg tattaacacg attccattct tcacctttta ctctatgttt gggtttcaaa
     8941 gaataggtga ccttatttgg gcggcaggag atatgcgctg ccgtggattt ttggttggtg
     9001 gtacttctgg aggtactaca ctggctggag aaggacttca gcatcaggac gggcatagtc
     9061 atcttttagc ctatccagtt cctaatctca aggcatacga cccagccttt gcttatgaaa
     9121 tagccgtcat cattcgggat ggtattcacc atatgtatga agaacagcag aatatcttct
     9181 actatctaac cgttacaaat gaaccctacg tgcagccgcc catgcccgat ggtgtaaaag
     9241 aagggattct aaaagggttg tataagttca aggcttcgga taataagaat gcaaagcttc
     9301 acgcgcatct tttcggcagt ggcgccatct tgaatgaagc gctgaaggct cagaagatat
     9361 tagaagagaa atacgaaatc gctacagatg tatggagcgt aaccagctat aaggaacttt
     9421 atcgtgaagg gcacgaggca gagcgctgga atatacttca cccctccgag ctacctaaaa
     9481 tcccatacgt caccaaatgc cttcaggatg cgtcaggcgt atttgtagcg gcttcggact
     9541 acgttaaggc gattcctgac tccattactc ggtggttacc taagcccatt gtatcgctcg
     9601 ggaccgatgg ctttggcaga agtgacagcc ggcgagcgtt gcgtgacttt ttcgaggttg
     9661 attcaagatt catcactcta gctacgctga ctgccctggc gcgggagaag gaaatcaaaa
     9721 ccgatgttgt gcaacaagca ataaaagacc ttgagattaa cccagcgaag gctaacccaa
     9781 tgatttcata aaaaggtaat tattcaggca caaatttgca caaaagaaca caaatgaaag
     9841 aaaaattctg attagtcatt gaggtcacgg aggagacaga aaaagatttt ttaagaaaga
     9901 attctgttaa ctctgtgttc tctgtggcta aataaatcca acgagaggaa attatggtag
     9961 aggaat
//
LOCUS       DATDRL010000013         4738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_771162, whole genome shotgun sequence.
ACCESSION   DATDRL010000013 DATDRL010000000
VERSION     DATDRL010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771162"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VI935_00550"
     CDS             <1..433
                     /locus_tag="VI935_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24118.1"
                     /translation="KMFQLANNKKSSVIIVRESDGTGGCICCSGGMGEFAKKDHSYGK
                     IRKIAEECGRLHREITDRFGDEEVEIIQIEPRAYFYLMPKVLKDVLKFKPSILSTIRT
                     IFLWYPVPAVIVNGKPVGLGRIPTINEVLKELEHSKANPVN"
     gene            728..1915
                     /locus_tag="VI935_00555"
     CDS             728..1915
                     /locus_tag="VI935_00555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HLE24119.1"
                     /translation="MKSKFFKTTFFVVAKLFALGFLLQSVSHAQADIEMVPGEILVKF
                     NAGADVRNIHAANGTRTLEVIPRINVHVVGLGHGVDPIAAVERFNKNPNVEYAEVNGL
                     YYVIHTSGVSVPNDTYFDSDQWNMYSINATSAWYVTKGSNTVKIAILDTGIQSNHEDL
                     SGKVVLSKNYSKSRTLQDKYGHGTHVAGIAAAATNNGKGVAGVGYNCSLMNVKVLNDK
                     GSGTWSGIANGIIWAADNGAKVINMSLGGYTGSSTLENAVNYAWGKGVVVVAAAGNEE
                     TNTPLYPAYYGNVIAVAATDFLDELAYFSTFGNWVDVAAPGDFIPSTYPLPYPYNYVY
                     LSGTSMASPHVAGLACLLASQGRSRDNIRAAIENTTLYKGSYPIANGLIDAHKAVDYL
                     IVP"
     gene            1964..2470
                     /locus_tag="VI935_00560"
     CDS             1964..2470
                     /locus_tag="VI935_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HLE24120.1"
                     /translation="MGFTHCSSVTHFVKLLIVIPFLVGCARQEIISAKEVPKTLSSVQ
                     NTEGLKVELNLNKPSYKLKEPTIMILTVTNKSKETCRYSFRSSQVYDFVVKEDNKEIW
                     RWSADEVFAQVITELTLAPGKSESFEEIWEQVDNEGKNVRAGKYQVIGILATQPERIS
                     HPVSIEIR"
     gene            complement(2577..3506)
                     /gene="lipA"
                     /locus_tag="VI935_00565"
     CDS             complement(2577..3506)
                     /gene="lipA"
                     /locus_tag="VI935_00565"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HLE24121.1"
                     /translation="MIQTLRKPSWIKAKIPAGSNYTRLKNLMRSLNLHTVCEEAQCPN
                     VGECWGTGTLTFMILGDTCTRSCGFCAVKTGKPGDIDWNEPERVAKAVRELTLPDAYL
                     THVVITSVNRDDKNYESALIFADTVRKVRKYNPEITIEVLIPDFKGDIKALKVILDAK
                     PDVLNHNVETMPRLYYLPVETESGLKRSVRPQARYEWSLRILEHGKEFGHQGLLTKSG
                     IMVGLGEEIEEIIETLWDLKNARCDVVTIGQYLRPSLDQLPVVRYYHPLEFESLKKYG
                     ETIIGIPHIEAGPLVRSSYHAEKQVMKLVGNFI"
     gene            complement(3705..4061)
                     /locus_tag="VI935_00570"
     CDS             complement(3705..4061)
                     /locus_tag="VI935_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24122.1"
                     /translation="MRFIRRLFAFSIIAIGLLSAEAIGVEGVAPNNGWDYEGQLIVIT
                     ASTGSMAQYCGLSLMEYNAEVIKPSGMGFGFITMAFSVDSNVQTGMHFKVIDQNECSI
                     DPSRLILVLQQIDMMD"
BASE COUNT         1405 a          974 c          970 g         1389 t
ORIGIN      
        1 taagatgttt caactggcta ataataaaaa gtcatctgtg ataatagtga gagagagtga
       61 tggcacaggt ggctgcatat gctgctcagg cggaatgggt gagttcgcaa aaaaggatca
      121 ctcttatgga aaaattagaa agatagccga ggaatgtgga aggctccatc gagaaatcac
      181 cgatagattt ggggatgaag aggttgaaat tattcagatt gaaccaaggg cttatttcta
      241 tttgatgcca aaggttttaa aggacgtttt aaaatttaaa ccctctatac tttctaccat
      301 ccgcacaata ttcttatggt atccagttcc ggctgtgatt gtaaacggca agccagtagg
      361 cttaggaaga atcccaacga ttaatgaggt tttaaaggaa ctagaacatt ccaaagcaaa
      421 tcccgttaat tagtttgtta tattttgtaa caaacctgta atagtcttta attattggat
      481 ttcttatctt ctacttgccc aaggttacat ttgaagttta aagatttgtt tttcccatat
      541 aatagaatgg ttataggaga aaaggcgaat tatagggtta aagtgatacc agaaatgata
      601 caaaaaacct attgacattt tctttaaatg ggattataca ataagctgta taaatgttta
      661 tgcataaaat cgggtaataa cttttggcat aatacgggtt aaaggttata taaaggaggt
      721 aacgaggatg aaatccaaat tttttaaaac cacctttttt gttgtagcta aactttttgc
      781 tttaggcttt ttactccaat ccgtctcaca tgctcaagca gacatagaaa tggtgcctgg
      841 agagatcttg gtgaagttta atgcgggcgc cgacgttcgt aacattcacg ccgccaacgg
      901 cacaaggact ttggaggtta tccctagaat caacgtgcat gttgtgggtt tgggccatgg
      961 cgtagaccct atagctgcgg tagagagatt caacaagaat ccgaacgtgg aatatgctga
     1021 ggtaaacggg ctctactatg ttatacacac ctctggcgta tccgtcccta acgacacata
     1081 ttttgattct gatcagtgga atatgtactc cattaacgct acttctgcat ggtatgtcac
     1141 gaagggaagc aacaccgtca agatcgcgat tctagacact ggcatccagt cgaaccacga
     1201 ggatctgagc ggaaaagtcg tactttcgaa aaattactct aagagcagaa cccttcagga
     1261 taaatacggt catggcactc atgttgccgg aattgcagcc gctgcgacaa acaacggcaa
     1321 aggggttgca ggcgttggat ataactgcag tctgatgaac gtcaaagtct taaacgacaa
     1381 aggcagcggt acatggagtg ggatcgccaa cggcatcata tgggctgctg ataacggggc
     1441 aaaggtaatc aacatgagcc ttggcggata cactggttct tcaacacttg aaaatgcggt
     1501 taactacgct tggggcaagg gcgtcgttgt agttgccgca gcgggaaatg aagaaactaa
     1561 tactccactc tatccagctt actatggtaa cgtgattgcc gttgctgcta cagatttctt
     1621 agacgaactc gcgtactttt ccaccttcgg aaattgggtt gacgtggcag ctcccggaga
     1681 ttttataccc tccacatatc cattaccata tccatataac tacgtgtatc tgagcggaac
     1741 atctatggct tcaccgcatg tagctggctt ggcttgccta ttagcctcac agggaaggag
     1801 cagagacaat atacgagcgg ctattgaaaa taccaccctc tataaaggct cctatccgat
     1861 tgcaaacggg ctaatcgacg cccataaggc tgtggattat cttatcgttc cataatagct
     1921 gagtgtgaaa accatatgga gcccattata gcctcaagct attatgggct tcactcattg
     1981 ttcatctgtg actcatttcg taaaacttct aatagttata cctttcttag tcggatgtgc
     2041 tcgacaagaa atcatttcag ctaaagaggt tccgaagacg cttagttcag ttcaaaacac
     2101 agaaggctta aaagtcgaac tcaacctcaa caaaccctca tataagctaa aagagccgac
     2161 aatcatgatc ctaactgtaa ccaacaaatc caaagagaca tgccgctaca gcttcagatc
     2221 atcccaggtg tatgacttcg tcgtgaaaga agacaacaag gagatctggc gctggtcggc
     2281 ggatgaagta tttgcgcaag tcataactga acttacactt gcgccaggaa aatcggagtc
     2341 ttttgaggaa atttgggaac aagttgataa cgaaggaaag aatgttaggg ctggaaagta
     2401 tcaggtaatt ggcatattag caacccaacc tgaaagaata tcacaccctg tttctattga
     2461 aattcgttga cttagtttca tcataatact tgggtgagtt tttggagaat tcttctaact
     2521 gccgaatcca actctcgtct gtacacaatt tctaaattta acttagacta aaaatactag
     2581 atgaaattcc caaccagctt cattacttgc ttctccgcat ggtaggaact tcttacaagt
     2641 gggccggctt ctatatgcgg aattccgatt attgtctcgc cgtatttttt tagtgattca
     2701 aactcaagcg gatggtagta tctaacaaca ggaagttgat ctaaactcgg tcttagatac
     2761 tgcccgattg ttacaacatc acatcttgca ttttttaaat cccaaagcgt ttcgataatt
     2821 tcttcaatct cctctccaag cccaaccata atccctgatt ttgtaagaag gccttgatga
     2881 ccaaactctt tgccgtgttc caaaatccta agcgaccatt cgtaccttgc ctgaggtcgt
     2941 actgaacgtt tgagccctga ttcggtttct acgggaaggt aataaagcct aggcattgtt
     3001 tcaacattat gattaagcac atccggctta gcatctaaaa tgactttgag agcttttata
     3061 tctcctttaa aatcgggaat aagaacctca atcgttatct cgggattgta tttccttacc
     3121 tttcttacag tatctgcaaa tataagggcg ctttcatagt tcttgtcatc acggttgacg
     3181 gaggttataa caacatgggt tagataggcg tcaggaagtg taagctctct tacagccttt
     3241 gctacccttt ctggctcgtt ccagtcaata tctccaggct ttcccgtttt tactgcacaa
     3301 aacccgcagc ttcttgtgca agtatcgcct agaatcataa aggtaagcgt tcccgttccc
     3361 cagcactctc caacattagg acactgcgct tcttcacata cagtgtgaag attaaggctt
     3421 ctcatgaggt tttttaagcg ggtgtagttt gaacctgcgg gtatcttggc tttaatccaa
     3481 ctcggttttc gcaaggtttg aatcattttt gtaactgttt agccacagag atcacagaga
     3541 aatagttttt ttattaattg attttttttg ctttgtgtaa attgtgacta aatcatatcc
     3601 tgaatattta caatatttct aacatatatg gtagaggtaa gaaaaggtaa agtaaatatg
     3661 ttaaaaatgt gatactcaat attaacaaac aggctacttc aaatctaatc catcatatct
     3721 atttgttgaa gaacaagtat gagacggctt gggtcaatgg aacactcatt ttgatcaata
     3781 accttgaaat gcataccggt ttggacatta gaatcaacag aaaatgccat agttataaaa
     3841 ccaaaaccca tcccgcttgg ctttattacc tcggcattgt attccatgag tgagagaccg
     3901 cagtattgcg ccattgaacc tgtcgaagcg gtgattacaa tgagttgtcc ttcataatcc
     3961 caaccgttgt taggtgcaac gccttctact ccaatagctt cagctgatag aagacctatt
     4021 gctatgatag aaaatgcaaa tagccttctt ataaacctca tataagtaca cctcctcgtt
     4081 ctctaaatta tttaggtttt ttctttaatt tcaacctctt gttttttcat aactaaaata
     4141 actaatataa aagcagccag ggatatacca aataaagcaa cataccaccg ctaattgcat
     4201 gccttctcca ggtaatttaa gtttgttgat gatatacatg gcacaaaagc aatttcatgc
     4261 tatcttgcgg tttgttgtgt attggttgag actgaatggc tgccaacagc aaaaccgctc
     4321 tgagcctagt ccctgattta atcaggtaag tgacttgtaa actgccctac attttttata
     4381 tgccatgtcc ccgttcgcat gaaatcccct ctaactcccc tttaagaaag tggagaattt
     4441 tcaggtccct cttttttaaa gagggatcta gggagatttt aattcttcgt tacccccctt
     4501 tacgaaacga ggaaaataag tagagaggca ttgcaatgtt cttctactaa aactaaagtt
     4561 ctacctattt ccatcgaaaa acggtggagg gagaagggga ggttgttcct tttcttcgga
     4621 cgtgcgctga agctctatct ccgagagtaa aacgcttggt gcgatggcag agactgaaac
     4681 atagccagat tctgctccac agaatccgtt gaacacatcg tagtcatttg ctgtggct
//
LOCUS       DATDRL010000014        20153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2610823, whole genome shotgun sequence.
ACCESSION   DATDRL010000014 DATDRL010000000
VERSION     DATDRL010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 20153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20153
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2610823"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="VI935_00575"
     CDS             <1..1119
                     /locus_tag="VI935_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24123.1"
                     /translation="LKTIGETFIIENGFIQDKGRAKVHFTGRMENSLNGNRRLHLDIL
                     DFPILLIKTIFAPLLPKVIIDGQVKGSASLSLTSNHFLQEEYSFDGSLNIKGVSFTGR
                     YNDNYFYINGINGTILLKDKIKPENPLASLMGERLTLDQRVFRGFIDAVTPDYLNKEG
                     EFLSIREVEYGFLRIEDIECDVELDRSKLNIKRFETILYKGKVFGTSLIDFSGGGPKY
                     STSLLFKGISLQSITDSIPSIKDYITGRINGIIWLTGNGTKLNTIDGPFHFWDIKSKD
                     EDRRIGAAFLKHLGAKEKFFFGTSHKYDNAEINGYVKDGVITFKELDISHTFFGFNTL
                     SVRVDERRNSISAAHFLSVIREMARRAGAGRLKIQFEN"
     gene            1140..1847
                     /locus_tag="VI935_00580"
     CDS             1140..1847
                     /locus_tag="VI935_00580"
                     /inference="COORDINATES: protein motif:HMM:NF018701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HLE24124.1"
                     /translation="MMRVRTMVFQVVVISVSIYLLSCATVTVNVYFPAEEVRQAYETL
                     EEELLKPPQEETPGTEKAPKQGEIEKKPQSILKYPDKPQLQSRRLIILTRKFSLDLGT
                     FAWAQDDLDKAIVNEIRNMPDVVQAYRNRGGRLDVINNMLSQGKVGIGNKGLLVDRGG
                     LTKEEKADFNAENKDRETIIEGMATAIIKINNIDPTPQNIEKVIPEAAEQFAAVRRNE
                     AQPGWWIQLPDGRWMQK"
     gene            1873..2880
                     /locus_tag="VI935_00585"
     CDS             1873..2880
                     /locus_tag="VI935_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLE24125.1"
                     /translation="MSSPSKIGIVIPYWSNITKSELIEFARLVEELGYDSIWAEETWG
                     RDAFSLLTLLALNTKKVKLGTGVVSVFSRTPALIAQTIATLDEISEGRMILGLGTSGP
                     IVIKDWHGLEFEKPLQRTREYVEIIRMILSGERVDYDGGIFKLKNFRLQFTPIRKNIP
                     IYIASLGPKNIQLTGELADGWIPFLVPPGFLRDARKELEVGAKAKGRDLDQVKVCPYI
                     PACISKENDFPKRVIQEFIAYYVGGMGTFYYKTLSKLGFQNEAELIVKAWKRGSKNQA
                     VQAVSDKMLNSIAILGTKDEGKKKLKEYRLAGADLPILISPPKSSRDMVKETIRGLAP
                     A"
     gene            complement(2904..3848)
                     /locus_tag="VI935_00590"
     CDS             complement(2904..3848)
                     /locus_tag="VI935_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLE24126.1"
                     /translation="MARQLRLEFPGAIYHITSRGNARQEIFLDDEDRRSFLYLIGKEM
                     EQQRWRCYSYCLMNNHYHLVIETPEGNLISGMRRLNGVYTQMFNRRHGRVGHLFQGRY
                     KSIIVDREGYLLELCRYVVLNPVRAGIVEGPEQWGWSSYNCTVGLREGLSWLDTKWIL
                     EQFSYDEKSARDQYRRFVLDGIGGPRPWEGLKGQIWLGSTEFLEKMDRLINKKSIKNV
                     PITQTLPARPTKFEVIESVARVYDMSIDDVIYRRDAGAYKAAVYLLRRVVNLGLKNVA
                     EEFGISTTRVSKIQGEIEGIDKIDPKLIRLLRKYKVKK"
     gene            complement(4114..5781)
                     /locus_tag="VI935_00595"
     CDS             complement(4114..5781)
                     /locus_tag="VI935_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HLE24127.1"
                     /translation="MLNPLFNKNGGQRQLSYDKYNLMFGRLITLLILAVLLLQCEDRE
                     KESLSIALPNRDSLNINIGSDPPTLDWSLATDSTSYTIILNIMDGLTRFGKDYKPEPS
                     LAESWDISEDGKTIVFHLRKNVSWSDGRPLKAKDFEYSWKRLLNPKTGADYAYFLYDI
                     EGAEEYNTGKEDDPEKVGVKAIDDSTLLVKLRRPASYFLSLLTFMSTFPMREDVVKKH
                     GLKWTEPKNIVTVGPFRLSNWRHHEKILLTKNPNYWGEKPKLTEVEMIMNENPTSALA
                     LYEGGELDYIDGRSIPTLEVPRLRLSREFNTEPQFRGNYIGFNAKKPPFDNPLVRRAF
                     SAAIDRAKIAELIQGAGVPTTSWIPQGMLGYDSEIGIEFNPEQARAWLAEAGYPDGNG
                     FPEVLFLYPDVGNNRIVSEALQSMWRRYLGVDVELQNQEWKVYLSTLDTDPPQVFRAN
                     WGADFPDPHNFMNLFECNSGNNETRWCNKLYDELVEKAAVEENPEKRVALYKEAQKIL
                     TEADVPIAPILRSIQQSMIKPYVVGLNLNPLGMVFFNQVSFADKISN"
     gene            complement(5996..6889)
                     /locus_tag="VI935_00600"
     CDS             complement(5996..6889)
                     /locus_tag="VI935_00600"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HLE24128.1"
                     /translation="MKEEIVGVTGGTGFVGRNIVKKLLEDKWKVRCLVRKPEGSENLK
                     KQGIEIYKGDITNKSSIDKFFLQDLSYLIHLVGIIKEVETQTFERIHFQGTKNLVDLA
                     KDSSVKKFIHMSALGTRPDARSRYHSTKWMAEEYLRKSGLNYTIFRPSIIFGPNDEFI
                     NTFIRIIKISPFIPVIKGGKLQPIYVGDVARCFIQALNNPKTDGKTFELGGTKKLTLE
                     EIIKALLRVLNVERVLIPIPSFLMYPPAYLFQKVLRNPPLTLDQLIMLEEENACDMKE
                     VQIIFSFSPLPLEEGLTQYLS"
     gene            complement(7088..7618)
                     /locus_tag="VI935_00605"
     CDS             complement(7088..7618)
                     /locus_tag="VI935_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HLE24129.1"
                     /translation="MMYTFKFQDQTYKIKLEEEGKQTEIEIAGNSEANRQSSFAERKI
                     PLEFQKIDESFYSIIVDGRSIGVGILKKGKRVEVFVGGDLYEFESIAEREREITKGVI
                     LGVQEIKSPMPSRVVKILKANDDGVKEGEGVIVIEAMKMESELKSPIEGKVKEVKVKE
                     GDAVESGTVLVLISSD"
     gene            complement(7615..9111)
                     /gene="accC"
                     /locus_tag="VI935_00610"
     CDS             complement(7615..9111)
                     /gene="accC"
                     /locus_tag="VI935_00610"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HLE24130.1"
                     /translation="MFNKILIANRGEIAVRIIRACRELGVKAVAVYSDADRRSLHVTM
                     ADEAYHIGSPAPGESYLVIEKIIDVIKRSGAEAVHPGYGFLSENHAFAEMCEKEGIVF
                     IGPTSEAIRLMGDKVTARRIAQEAKVPLVHGSEGAVSDVEAEVIAKRIGYPIMVKASA
                     GGGGKGMRLVRKEEDFKASLRMARSEARSAFGDDSVYIEKFIERPRHVEIQVIGDSYG
                     KVLHLFERECSVQRRHQKVIEEAPSPAIGDDTRRKMGEVAVRVAKAVSYRGAGTVEFI
                     LDQNGNFYFLEMNTRVQVEHPVTEMITGFDIVKWMIRIAAGEKLPFSQDDLSINGHAL
                     ECRVYAEDPEKNFLPSPGKLEYLRTPSGPGVRDDSSIYNGYEVTPFYDPMLSKLIVWG
                     GTRHEAIKRMRTALCEYIVLGVKTNIGFLRRIMENEEFIRGEIDTSFIERHPELLPPS
                     LGEIEPALIAAALAMRNSTKAVETSKQAPASNWKLLSRKLAVSRNPLI"
     gene            complement(9230..10774)
                     /locus_tag="VI935_00615"
     CDS             complement(9230..10774)
                     /locus_tag="VI935_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HLE24131.1"
                     /translation="MSDKIAFLEEKEKEAELGGGAVRIQKQHDMGKLTARERINLLLD
                     KNSFVELDKFVVHRCTDFDMEKNRFLGDGVVTGYGKIDGRQLFVFAQDFTVFGGSLGM
                     VHSKKICKIMDLAMKVGAPVIGLNDSGGARIQEGVESLAGYAEIFLRNVLASGVVPQI
                     SAVLGPCAGGAVYSPVMTDFILMVKDTSYMFITGPDVIKAVTHEEVTREELGGAMIHN
                     STSGVAHFATSDDRECIQVIRELLSFLPSNNMEDPPRISCDDPPDRHDMRLKDAVPEN
                     PNKPYDIKDVIRGVVDDGYFFEIQEHFAQNIVIGFSRLAGRVVGIVGNQPNVLAGCLD
                     INASVKGARFVRFCDCFNIPLLTFVDVPGFLPGTAQEWGGIIKSGAKLLYAYCEATVP
                     RVTVITRKAYGGAYDVMSSKHIRGDINYAYPTAEIAVMGPEGAVNIIARKEIEQAENP
                     EAERARLANDYRNTFANPYRAAELGYIDQVIRPEDTRPRVIGAFEMLEGKRETNPPRK
                     HGNIPL"
     gene            complement(11440..12036)
                     /gene="coaE"
                     /locus_tag="VI935_00620"
     CDS             complement(11440..12036)
                     /gene="coaE"
                     /locus_tag="VI935_00620"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609833.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HLE24132.1"
                     /translation="MKVIGLTGNIACGKTVVARMFEELGAKIIDADYIARLIVEPGEP
                     AWKDIVEKFGHKILNPDGTINRKKLGDIVFRDIEKREELNRTTHPRIIDKIESMLEEY
                     RKENIKVVIVEAALILEKGGMKPIISDLMVVAADEEAQIKRLIERDGISREEALTRIK
                     AQMPVSEKVKFATYVIDNSRTLEETRKQVEEVWKKINA"
     gene            12494..13357
                     /locus_tag="VI935_00625"
     CDS             12494..13357
                     /locus_tag="VI935_00625"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HLE24133.1"
                     /translation="MALKVEDVKKVGVVGAGTMGSGIAQVVASNGRNVILLDLSDSAL
                     DRGMKAIGNSLGRLVKKGDINEGDSKSILSRIMTTTRFEDLADTDLVIEAVFEDLSVK
                     NETFKKLDATTRPDIMLATNTSSLPIIEIAVSTKRPDKVIGMHFFNPVPLMKLVEIIK
                     SLATSEETVQFAYDFAMALGKEPVNAKDVPGFIVNRVLMPMLNEAVFALDEGVGTPED
                     IDKAMKLGTNQPIGPLALIDLIGLDVTLDVIDVLYREFKDPKFRAAPLLRQMVRAGWL
                     GRKTGRGFYKY"
     gene            13890..14456
                     /locus_tag="VI935_00630"
     CDS             13890..14456
                     /locus_tag="VI935_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017323030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLE24134.1"
                     /translation="MGQVKTSKGNKVLHKRSGGNQNTKPAAVALSKAYFGDSALASLI
                     GEVVALFHRLRIVSEQIHRQGEMTSGKRGILGSLDRFGPQTVPEMARARPVSRQYIQT
                     LVNQLMHEGLVEFIENPAHKRSHFVRLTPKGKELLDSMIQRETELLSQLKLDIPEKAL
                     HDGAGVLKAVRELFESKQWKELLKEDGV"
     gene            14453..15568
                     /locus_tag="VI935_00635"
     CDS             14453..15568
                     /locus_tag="VI935_00635"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HLE24135.1"
                     /translation="MKVINRILRPATWVFYLVIVCEILFMISPFALYFYSAYGPTLNF
                     LHHSPYAAWLTQFFLPHFSETSSPLLNALPKLGGFLIVIGVLMFFAGAIPIYWAKIRG
                     RGAVTGGLYKFIRHPQYVGLAIVGLGTVLVWPRFLVLITYVTMLFLYDLLARLEEERC
                     LTKFGETYRAYKARTGMYLPQALSTSIPRILPPSGPKRVLATIGIYIVVISATVTFAF
                     SLRDDSLRKISSFYTDDIAVLSPATLTDEELRAAFNIAMAEDEVQNKLRTDGAAKLIV
                     YVVPQEWYIPDLPIESEYKPGGHHVPQDFDRRYYKLLFTKPRTHTPEATARNIVKSAY
                     GRDPIVLVKVDILSSKLIGIETPPSHVYWGDIPTPMF"
     gene            15672..16139
                     /locus_tag="VI935_00640"
     CDS             15672..16139
                     /locus_tag="VI935_00640"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HLE24136.1"
                     /translation="MNIVLISLTVITGIVLISALGFFGTLRGKESREERKERAVEWVS
                     SELDLTKEQRSKLVHISDELSNLENEMKKDRDELKEEFINSIASDELDQAKILEWVKE
                     KQKLVDEFAPFIIAELADFHKSLNPEQRKKLADELNKHSHENRFGHGLYHGNH"
     gene            complement(16212..17648)
                     /gene="gltX"
                     /locus_tag="VI935_00645"
     CDS             complement(16212..17648)
                     /gene="gltX"
                     /locus_tag="VI935_00645"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HLE24137.1"
                     /translation="MSVRVRFAPSPTGALHIGGVRTAIFNWLFARHHSGKFILRIEDT
                     DRTRSTEESIAEILDAMKWLGLDWDEGPFRQSDRLSIYQSNAQKLIQVGKAYRCYCSQ
                     EELEIRRKEAEKQGKVYRYEGICRNTFDRLATGLKSPPSHKSYAIRLMTPEIGSIEVQ
                     DVIRGTITFDAKEIDDFIIQKTDGFPTYNFAVVIDDATMDITHVIRGDDHIANTPKQV
                     LIYEALSQEIPKFAHVSTILGSDKAKLSKRHGATSVVAYREMGYLPEALVNFLTRLGW
                     SYGDQEIFTKQELIEKFTLENVGKSPAVFNPEKLLWLNGWYIRNKPAEEIAQLVIPFL
                     EKKGFQVILDERLIKIVNILRERAKTLVELAISSYYFFIDEIKYEEAARKKFLTPEVL
                     PIFEALIERFSSLDEFTDPELQKIFGEIIEKRGLKLVQIAQPVRVALTGGTVSPGIFE
                     VMEIMGKEKVKGRLKKAVHNIKSLNPPL"
     gene            complement(17788..18039)
                     /locus_tag="VI935_00650"
     CDS             complement(17788..18039)
                     /locus_tag="VI935_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004813145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HLE24138.1"
                     /translation="MSTVVIYTTTYCPYCRAAKRLLESKGVNYEEFDLTENEEIKTKV
                     MEELGWQSVPIILINEKLIGGYDQLRELEREGKLDEMFK"
     gene            18269..18892
                     /locus_tag="VI935_00655"
     CDS             18269..18892
                     /locus_tag="VI935_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HLE24139.1"
                     /translation="MFRFFPKEEKFFDYFDDASEKMIRGIKLLKEMVQDLSNAEEKAK
                     QIKDVEHEADHITHETVSKLHKTFITPIDREYIYALITKMDDILDLIDATCERIILYK
                     IKSSTPEVIALVNTLEKAIEEVAKGIRGLRNLENSRSILDICIEINRLENEGDRTYRN
                     AQSVLFNSPSDPIEIIKWKDIYETLEEAIDTCEDVANIIEGIVLENA"
     gene            18885..19883
                     /locus_tag="VI935_00660"
     CDS             18885..19883
                     /locus_tag="VI935_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HLE24140.1"
                     /translation="MLDLHLSFIIFVIIVALFFDFTNGIHDAANSIATIVSTRVLTPK
                     QAVAWAAFFNFVAFLIFGTSVAKTVGKGLIEISTVTPAVVFAGLIGAIGWNLTTWYLG
                     LPTSSSHALIGGYAGAAIAKSGIGVIIASGWYKTLVFIILAPTIGLLLGMILIIIATW
                     LVYKETPAFVNKWSRRFQFLSAALYSLGHGGNDAQKTMGIITGLLFSGGLISKFAVPL
                     WVVLSAHAVIGLGTLVGGWRIVKTMGQKITKLRPIDGLCAETASAASIFLATYLGIPV
                     STTHVITGAISGVGASKGLSTVRWGITLRIVWAWIFTIPAAAFIAGLAYNILNFFT"
BASE COUNT         5720 a         4275 c         4025 g         6133 t
ORIGIN      
        1 cttaaaacaa ttggcgaaac ttttatcata gaaaatgggt ttatacagga taagggaagg
       61 gcgaaggttc acttcacagg cagaatggaa aactctttaa acggaaatag gaggcttcac
      121 ctcgatatac tggattttcc catattacta atcaagacaa tctttgctcc tctactaccc
      181 aaagtcataa ttgatggtca ggttaaagga agcgctagct tatccttaac ttcaaatcac
      241 tttcttcaag aagaatattc atttgatgga agtttaaata taaaaggcgt ttccttcaca
      301 ggtaggtata acgataatta tttttatatt aatggaataa atggaacaat cttgttaaag
      361 gataaaatca aaccagaaaa ccctctagcc tctttaatgg gagaacgctt aacactggat
      421 caaagggtat ttagaggctt tatagatgcc gttaccccag attatctgaa caaagaaggg
      481 gaatttctaa gtatcagaga agtagaatat ggatttttaa gaattgagga tatagaatgt
      541 gatgtcgagt tggatagaag taaactcaat ataaaacggt ttgaaaccat actttataaa
      601 ggcaaagtat ttggcacaag cttaatagat tttagtggag gaggacctaa atatagtacg
      661 tcacttctgt ttaaaggtat aagcctacag agtattacag acagcatacc ttcaatcaag
      721 gactatatta caggtagaat aaacggtatt atttggctca ctgggaatgg tacaaaactc
      781 aatactattg atgggccttt tcatttttgg gatatcaagt caaaagatga ggacagaaga
      841 atcggggcgg cgttcttaaa acacctcggc gcaaaggaaa aattcttttt tggaacatca
      901 cacaaatacg acaatgccga gattaacggt tatgtaaagg acggagtgat tacatttaaa
      961 gagcttgaca tttctcacac ctttttcggc tttaatactc tttccgttag ggtggacgaa
     1021 agaagaaatt ccatatctgc agctcatttt ctttcagtta taagagaaat ggcaaggagg
     1081 gctggtgcag ggaggcttaa aatccaattc gaaaattaaa taacagaggt aaaataaaaa
     1141 tgatgagagt aagaacaatg gtatttcaag ttgttgtaat atctgtgtct atttaccttt
     1201 tatcttgcgc aaccgtaaca gtaaacgtct actttccggc ggaagaggtg agacaggctt
     1261 atgaaacatt agaagaggaa cttctaaaac ctcctcaaga agaaacccca ggaactgaga
     1321 aagctcctaa acaaggtgag atagagaaaa agcctcagtc cattcttaag tatccagata
     1381 aaccacagct tcaatctaga aggttaatta tccttacgag aaaattctcg ctagaccttg
     1441 gcacgtttgc gtgggcacag gatgatcttg acaaagccat tgtaaatgaa attagaaata
     1501 tgccggatgt ggtgcaagca tacaggaata gaggggggcg ccttgatgtc ataaacaata
     1561 tgttatcaca gggcaaggtt ggaataggga ataaaggact tctagtggat aggggtggtc
     1621 tcacaaaaga agaaaaggca gatttcaatg ctgaaaataa agatagagaa actataattg
     1681 agggaatggc tacggctatt attaaaatta ataacattga tccaactcct caaaatatag
     1741 aaaaggtgat acccgaagcg gctgagcagt ttgctgcggt gagaagaaac gaagctcaac
     1801 ctggatggtg gattcagctt cctgacggca gatggatgca aaaatgaaac gcctaatcct
     1861 attggattaa taatgagtag tccatctaag atcggtattg taattccata ctggagcaat
     1921 atcaccaagt ctgaacttat tgaatttgca aggcttgtgg aagagcttgg ttatgactct
     1981 atttgggctg aagagacatg gggaagagat gctttctcac tacttacact acttgctctt
     2041 aatacaaaaa aggtaaaact tggaacaggg gttgtatccg tatttagccg aaccccagca
     2101 ttaattgctc aaaccattgc tacgctcgat gaaatttcgg aaggacggat gatcctagga
     2161 ttaggtacaa gcggtcctat cgttataaaa gactggcatg gattagaatt tgaaaaaccc
     2221 cttcagagaa caagagaata tgtagaaatt ataagaatga tcctaagcgg agaacgggta
     2281 gattacgatg gcggaatatt taaactcaaa aattttcgcc tccaatttac acctataaga
     2341 aaaaatattc ctatttacat cgcttccctt ggccccaaaa acattcagct tactggggag
     2401 ctagcggatg gatggattcc ttttttagtt ccacctgggt ttcttcgtga tgcaagaaag
     2461 gagcttgaag ttggagcaaa ggctaaggga agggatttgg atcaagtcaa ggtgtgtcct
     2521 tatattccag cctgtatctc gaaggaaaat gattttccaa aacgcgtaat tcaagaattt
     2581 atagcttatt atgtgggagg gatgggtaca ttttactata aaacattaag caaacttggc
     2641 tttcaaaatg aagcagagct tattgtgaag gcatggaaga gagggagtaa gaatcaagcg
     2701 gttcaagctg taagtgataa gatgctaaat tctatcgcaa tacttgggac taaagacgag
     2761 ggcaagaaaa aactaaaaga atacaggctg gctggagcag atttaccaat acttatttcc
     2821 ccacctaaat catctcgtga tatggtaaag gaaacaatca gggggcttgc accagcatag
     2881 aaattaaagg aaattaaata gggtcatttc ttgactttgt acttcctcaa cagtcttatt
     2941 aattttggat cgattttgtc tattccttca atttctccct gtatcttgga tactcttgtt
     3001 gttgatatac caaattcttc tgccacattt ttgaggccca gattaactac cctcctaagc
     3061 aaataaacag ccgctttata cgccccggca tctctacgat aaattacatc gtctatactc
     3121 atatcatata ccctcgccac tgattcaatt acctcaaatt tagtcggcct ggcgggtagt
     3181 gtttgagtta ttggtacatt tttaatgctc ttcttattta ttagcctatc catcttctct
     3241 aaaaattccg tactccccaa ccaaatttgg ccctttagac cctcccatgg gcgtgggcct
     3301 cctatgccat ctaatacaaa acgtctgtat tgatcacgag cacttttctc atcataacta
     3361 aattgctcca aaatccactt tgtatctaac caactcaacc cctctcttag accaacagta
     3421 caattatagc tactccatcc ccactgctca ggcccttcaa caataccagc ccttacaggg
     3481 ttcaaaacta cataacgaca taactccaat aaatagccct cccgatcaac gattatactt
     3541 ttatatcgtc cctgaaataa atgccctact cgtccatgtc gcctattaaa catttgagta
     3601 taaactccat tcagccgtct catcccagag atcagattcc cttccggtgt ctctattacg
     3661 aggtggtaat gattgttcat aagacagtag gaatagcatc tccatctttg ttgttccatt
     3721 tctttaccaa ttaaataaag aaaactcctt cgatcttcat cgtccaaaaa gatctcctgt
     3781 ctggcgtttc cacgtgatgt aatgtgatat atagcaccgg gaaattctaa tcttaactgc
     3841 cttgccatga tattgtgaaa gtatactatt agtacaaagt caagcaatga cccttagagt
     3901 ccttataagg gcgccccccg gatttaatcg tgagacccat ttgcccaact ttttacattc
     3961 cttcgactgc cactgccgcg acttcggtat agcgagttga aaaacccctc ccacataatt
     4021 agggcagaca caggggtctt cccctacatt aataatttga taatttgtaa taagataggc
     4081 cgacagccct agtaaagtta aatgcaaaca gcactagttt gagatcttat cagcaaaaga
     4141 aacctggttg aaaaaaacca taccaagtgg attgaggtta agaccgacca cataaggttt
     4201 tatcatactc tgttgaatcg atctcaggat aggggcaatt ggaacatctg cctctgttag
     4261 aatcttctgt gcttctttat aaagggcgac acgtttttca ggattctctt ctacggctgc
     4321 tttttctaca agttcatcgt atagtttgtt acaccatctt gtttcgttgt ttccgctgtt
     4381 acactcaaag aggttcataa aattatgcgg gtctgggaaa tccgcacccc agttggcgcg
     4441 aaacacctgg ggagggtcag tgtcaagtgt gcttagatag accttccatt cctgattctg
     4501 tagttctaca tctactccaa gatatctcct ccacatgctt tgcaaggcct cggatacaat
     4561 tctattattt ccgacgtcgg gatacaaaaa gagtacctcc ggaaagccat ttccatcggg
     4621 gtatcccgct tctgctagcc atgccctagc ctgctcagga ttaaactcga tcccaatttc
     4681 agaatcatat cctagcatcc cctgaggaat ccaagaggtg gtagggactc ctgcaccctg
     4741 gataagctca gcaatcttag ccctgtctat agctgcagaa aaggcgcgcc ttacaagagg
     4801 attgtcaaat ggcggtttct ttgcattaaa ccctatgtaa tttcctctga actgcggctc
     4861 tgtgttaaac tctcgtgaaa gtctcagtct tgggacttca agcgttggta tgcttcttcc
     4921 gtcaatgtaa tcaagctctc caccttcata gagggccaat gccgatgtcg gattctcatt
     4981 cattatcatt tcgacctcgg taagttttgg cttctctccc cagtagtttg ggttcttagt
     5041 gagtaagatc ttttcgtgat gacgccagtt ggaaagacgg aagggtccta cagtgactat
     5101 atttttaggc tcagtccatt ttaagccgtg ttttttaaca acatcctctc tcattggaaa
     5161 ggttgacatg aaggtaagaa ggctaaggaa gtaggaagcc ggtcgcctta atttcactag
     5221 gagtgtgctg tcatctatcg cttttacacc gaccttttcg gggtcatcct ccttgcctgt
     5281 gttatattct tctgcccctt caatatcata aagaaaatat gcataatccg cccctgtctt
     5341 agggtttagg agacgcttcc atgagtattc aaaatccttt gcctttaggg gccttccgtc
     5401 gctccatgat acattttttc tcaaatggaa aactatggtc tttccatctt cgcttatatc
     5461 ccagctctcg gcaagagatg gctcaggttt atagtctttt ccaaaccttg taagcccgtc
     5521 cattatgtta agtattatgg tgtatgaggt gctatctgtc gcaagagacc aatcaagcgt
     5581 aggaggatca gagccaatat ttatgttaag cgaatctcta ttcggtaaag ctattgataa
     5641 agattctttt tccctgtctt cacattgaag gagaagaaca gcaagtatta ataatgtaat
     5701 taacctacca aacataagat tatatttatc ataacttaat tgcctttgtc caccgttttt
     5761 gttaaaaagt gggttcagca taacatttta agttccgaaa atatagcgga aaccttcagt
     5821 ttctataata tctaccatta atttatcact atgtagcggc gaccttcagg tcaccacata
     5881 gaaatgtcat ggagggctta agccctcctc tagatttaga agtcatttct agaattcaga
     5941 tcacgactgg tggttccaca agctcatctt gagttagaca gaaatgatat attcatcaac
     6001 tcagatattg cgttaaaccc tcttctagag gaagtggtga gaatgagaat ataatttgta
     6061 cctccttcat atcacatgca ttttcctcct caagcattat gagctgatct agtgtcaggg
     6121 gagggtttct aagaactttt tgaaaaagat atgccggagg atacatcaaa aaacttggaa
     6181 ttggaattag gactctctct acattcagga ccctcaataa tgcctttata atctcctcaa
     6241 gcgttaattt cttagtgccg cctaactcaa acgtttttcc atcggttttc gggttattta
     6301 gagcctgaat aaaacagcgt gcaacatccc caacataaat gggctgtagt tttccacctt
     6361 ttattacggg gataaatgga gaaatcttta ttattcgtat aaaggtattt ataaactcat
     6421 cattggggcc gaagataatc gaaggtctaa atattgtgta gtttagaccg cttttcctta
     6481 aatattcctc agccatccat tttgtggaat gatatctgct tctcgcatcg ggccttgttc
     6541 caagtgcgct catatggata aatttcttta cactagaatc tttcgctaag tcaacaaggt
     6601 tcttagttcc ctgaaagtga attctttcaa atgtctgtgt ctctacttct ttgattattc
     6661 caactaggtg aattagataa cttaaatctt gtaagaagaa cttatcgatt gaggatttat
     6721 tcgtaatgtc tcctttatat atctctattc cttgtttttt taagttttct gacccctccg
     6781 gctttcttac aaggcacctt actttccact tatcttctag gagttttttt acaatatttc
     6841 ttccaacaaa acccgtaccg cctgtgactc ctacaatttc ctctttcatc ggctttaatc
     6901 atccttgaaa atctaatagg atataaaaat acggatagtc caccagtccc ctgtactgag
     6961 caaagtcgaa ggattgctgg tggacggcaa cgagcgagcc tgtcctgagt tgcaacgaag
     7021 ggactcgttg cctacccact aggagattgg ttaatcaaac tcctataatt acctatttaa
     7081 caaatcccta atcagatgaa atcaagacaa gaaccgttcc gctctcaact gcatctccct
     7141 ctttgacctt tacttctttg acttttcctt caatcggtga tttaagttcg ctttccatct
     7201 tcatcgcctc tatgacgatc acaccttctc cttccttcac cccatcatcg ttagctttga
     7261 gaatctttac tacacggctc ggcattggtg attttatctc ttgaacccct aagataaccc
     7321 cctttgttat ctccctttct ctctcagcga ttgattcaaa ttcgtaaagg tcaccaccca
     7381 cgaatacctc aacccttttc ccttttttta gtattcctac tccaatagac ctgccatcaa
     7441 ctatgattga gtaaaagctt tcatcaatct tttgaaactc aagtgggatc tttctctcag
     7501 caaaactgct ttgcctgttt gcctcgctat taccagcaat ttcaatctct gtttgctttc
     7561 cttcttcttc gagtttgatc ttgtatgtct gatcctgaaa tttgaatgtg tacatcatat
     7621 aagtggattc cttgaaaccg ctaattttct actaaggagt ttccaatttg aggctggtgc
     7681 ttgttttgaa gtctctactg cctttgttga attcctcatc gcaagagctg ctgctataag
     7741 cgctggttca atttcgccta aagagggtgg gagtaattcg ggatggcgtt ctataaagct
     7801 agtatcaatc tctccacgga taaattcttc attctccata attcttcgaa ggaaaccaat
     7861 atttgttttc acgccaagca ctatatattc gcagagtgct gttctcatcc tctttattgc
     7921 ctcatgcctt gttcctcccc atacaatgag ctttgaaagc atcgggtcat aaaatggggt
     7981 tacctcatac ccattgtaga ttgacgaatc gtctcttaca ccagggccgc ttggagtccg
     8041 taggtattcc agttttccgg gagagggaag gaagttcttt tccgggtctt cagcataaac
     8101 tctacactca agtgcatggc cattgataga taaatcatct tgactgaatg ggagtttttc
     8161 acctgctgcg attcttatca tccactttac aatatcaaat cctgtaatca tctctgttac
     8221 tggatgctca acctgaacgc gtgtgttcat ctccaggaaa taaaaattcc cattctgatc
     8281 aagaataaac tcaaccgttc cagcacctcg gtaggatacc gcctttgcta cccttacagc
     8341 aacctctccc atttttcttc tagtatcgtc cccaatggca ggtgatggtg cttcttctat
     8401 taccttttga tgtcttctct gaactgagca ttcacgttca aaaagatgta aaacttttcc
     8461 gtaggagtca cctattacct gaatttctac atggcgtgga cgctcgatga atttttcgat
     8521 atagactgaa tcatcgccaa aagcggagcg agcctcgctt ctggccattc taagagaagc
     8581 cttaaaatcc tcttcctttc ttacaagtct cattcccttt ccgccgccgc ctgctgaagc
     8641 ctttaccata atcggatagc caatacgctt agcaatgacc tcagcttcaa cgtcgcttac
     8701 cgctccttct gaaccgtgaa ctagtggaac cttggcttct tgggcgattc tccttgctgt
     8761 tactttatct cccataaggc gaatggcctc tgatgtggga ccgataaaaa cgattccctc
     8821 tttttcacac atctctgcaa atgcatggtt ctccgaaaga aagccgtatc cgggatgaac
     8881 tgcttctgcg ccggagcgtt ttataacatc aatgattttt tctattacca gatagctttc
     8941 tccgggtgca ggagacccaa tgtgataggc ctcgtctgcc attgttacgt gaagcgaacg
     9001 acggtcagcg tcggagtaaa ctgctacagc ttttactcct agctctctac aggcccttat
     9061 aattctgacg gctatctcac ctctattcgc aatgaggatt ttattaaaca tttatctttc
     9121 ctttttgtaa ttactagcca caaataaaca ccgattttca ctaattaagt gatgcaagat
     9181 acatgatgca agaaagttat atccatcttg tatcgtgcat cgtgcatcat cataatggaa
     9241 tgttcccgtg ctttcttggt gggtttgttt ccctctttcc ttcgagcatc tcaaaggctc
     9301 ctatcactcg tggtcttgtg tcttcaggcc ttatgacctg atcaatataa ccgagttctg
     9361 ccgctctgta tgggtttgca aaggtgtttc tgtaatcgtt agcaagcctt gccctctcgg
     9421 cttcaggatt ctcagcctgc tcgatttcct ttcttgcaat gatgttcaca gccccttccg
     9481 gacccattac tgcaatctcg gctgtagggt atgcgtagtt tatatctccc ctaatgtgtt
     9541 ttgaagacat aacgtcgtaa gcgccaccgt aggcttttct tgttatcacc gtaaccctcg
     9601 gtactgtagc ttcacagtag gcatagagaa gctttgcgcc gctcttgata atcccacccc
     9661 actcttgagc cgtgccagga agaaacccgg ggacatcaac aaaggtaagt agtggaatat
     9721 taaagcagtc acagaatctc acgaacctag ccccttttac tgaggcgttt atatcaaggc
     9781 aaccggcaag cacatttggt tgatttccta ctataccgac gacacgacca gcaagccttg
     9841 aaaaccctat aacgatattt tgtgcaaaat gctcttggat ttcaaaaaag tatccgtcat
     9901 caacgactcc acgaatcaca tccttaatat catagggctt attcgggttc tcaggtacag
     9961 cgtctttaag cctcatgtca tgacggtcag gtgggtcatc gcaggaaatc cttggagggt
    10021 cttccatatt gtttgacgga agaaagctaa gaagctcgcg tattacttga atgcactcgc
    10081 ggtcatcaga agtagcgaag tgcgctacac cacttgttga attgtgaatc atagctccac
    10141 ctagttcttc tctggtgacc tcttcatgtg taactgcttt aatcacatct ggtccagtga
    10201 taaacatgta gcttgtgtct tttaccatga gaataaaatc agtcattaca ggtgagtaaa
    10261 ccgctccgcc agcgcaaggt cccaagacgg ctgatatttg aggaacaacg cctgacgcaa
    10321 gtacgtttct taaaaatatt tctgcatagc ctgcaaggct ctctacgcct tcttggattc
    10381 tcgctccacc agagtcgtta agaccgataa caggtgctcc aaccttcata gctaggtcca
    10441 tgattttgca gattttcttt gagtgaacca ttcccaggga accaccaaag acagtgaaat
    10501 cttgagcgaa tacaaataat tgtcgtccat caatcttgcc gtagcctgta acaactccgt
    10561 cgccaagaaa tctattcttt tccatatcga aatcagtgca tctatgaacg acaaacttat
    10621 caagctctac aaaagaattt ttatcgagaa gaaggtttat ccgctctctt gccgtgagtt
    10681 ttcccatatc atgttgcttt tgtatcctta cagcaccacc acctagctct gcttccttct
    10741 ctttttcctc aagaaatgcg atcttatcac tcatttttta ctccttccct ttggctcgac
    10801 aaaaatctac tcttaagcaa taacgttggc aagtcaggtt tttatggatt tcttttcatg
    10861 agattttcat atgcctgtcc tgagtttatc gaaggagtgg atttaagcga aatctaattg
    10921 gaatcaaact ataccagata aaactatgtc aatcaaaatt atgtggttcg tgaatcgtga
    10981 ttagtgaatc ggaacgttcg gccgagccgt tcgctctgag gcttcgtagc gagctcagcc
    11041 gaacggctca cgacagggcc aagtcagacg tcactaagaa ttcgggagtc agcccacgaa
    11101 gaattcatcg gggacggaag ccagaagata aacttctgaa ttctgaagcc tcgactgatc
    11161 tcagcgaagt ctactgactt ctggattcaa atttagtgcc tttgtggttg aataattcag
    11221 cagcagcggg gtcttccata taggaatcaa aaagctaggt ttacttggcg cgttgtacca
    11281 tttgtctttc ccgcatgtct gaagcgggaa tctagtattt gaatgcatgg atgcccgatt
    11341 gcaacattcg ggcatgacaa agagtaaaat gacccaacac tgaattggat agtttgagca
    11401 attaaaaaat ccgccacccc tcatataatt acaaaaaatc taggcattaa tctttttcca
    11461 aacctcttca acctgctttc ttgtttcttc taaagttctg gaattatcaa ttacataggt
    11521 tgcaaatttc actttttcag aaaccggcat ctgagctttg attcgagtaa gtgcctcttc
    11581 cctcgaaatt ccatctcttt cgataagtct cttaatctgt gcttcttcat ctgctgcgac
    11641 aaccattaaa tcactaatga ttggcttcat ccctcctttt tctagtatta atgctgcctc
    11701 aacaatcaca accttaatgt tttcttttct atactcctcg agcatcgatt ctatcttatc
    11761 gatgattcta gggtgagtag tcctgttaag ctcttctctc ttttctatat ctctaaaaac
    11821 aatatctcct agcttcttcc tgttaattgt cccatcagga ttaaggattt tatgtccaaa
    11881 cttctctacg atgtccttcc atgcaggttc acctggttct acaatcaatc ttgcaatgta
    11941 atcggcatca ataatctttg ccccaagttc ctcgaacatc cttgctacaa cagtcttacc
    12001 acatgctata ttccctgtaa gcccgattac tttcatctcg tggtagttta atttgcctag
    12061 acctttgttt caaatattta atattagttc gatatattcc catttccaat ataagatggt
    12121 taaggttccg atagacggcg ttttctaagg ccgtctggga gagcttccaa tggaaaaggg
    12181 tttaaaatgc caaaagaatg tcattcccgc atgcctttag cgggaatcta gcatttaagt
    12241 cgtttatgga tacgcccccg atttaatcgg gggtaactgc actcgggtat gacaggttag
    12301 gagttgccag gagtcgaagc ggcttaacga aggaaatgtc ccgcctatag tggagggaaa
    12361 atgtttaacc agttaatcga agtgactcgc tgtctaccca gatagttttc tattcccggg
    12421 ttaataaata aaatgcgatc attaggtata atacttctca aacttctaat tttcaaaaat
    12481 caaaggaggt tctatggctt taaaggtaga agacgtaaaa aaggttggtg ttgttggagc
    12541 tggaacaatg ggttctggaa tcgctcaggt tgtggcttca aatggaagaa atgtgattct
    12601 cctggatctt tctgattctg ctcttgatcg tggtatgaag gcaatcggaa atagtcttgg
    12661 aagacttgta aaaaagggag atataaatga gggagatagc aaatctattt tatctcggat
    12721 tatgacaaca acaaggtttg aggatttagc agatacggat ttagttattg aagctgtttt
    12781 tgaagaccta agtgtcaaaa atgaaacatt taaaaagttg gatgcaacta cacgtcctga
    12841 tattatgctt gccacaaata catcctcgct ccccattata gaaatcgccg tgagtacgaa
    12901 aagaccagac aaagtaatcg ggatgcactt ctttaatcct gtccctctta tgaagcttgt
    12961 agagataata aaaagccttg ctacatcaga agaaactgta cagtttgctt atgattttgc
    13021 tatggctctc ggaaaggagc cggtaaatgc aaaagacgtt cctgggttta tcgtaaaccg
    13081 tgttcttatg cccatgctta atgaagcagt atttgctctt gatgagggtg taggaacacc
    13141 tgaggatatt gataaagcga tgaagctcgg aacgaatcaa ccaatcggcc cccttgcact
    13201 tatagaccta ataggtcttg atgtaactct ggatgtaata gatgtactct atagggaatt
    13261 taaagaccca aagtttcgtg ccgcaccgct tctacggcag atggttcgtg caggatggtt
    13321 aggaagaaaa acaggaagag gattttataa atattgagct ttataattgt aatgtagcgg
    13381 atttttttcg acatagaccc atggacggtt ctcataaggg cgaacggccg tttcgcccct
    13441 acccatctac atgacttcga cagacacagg ggcaacttcg gtatggccgg gttggaaaac
    13501 cccacctatc cttattggtc gtaaaattta ttatcaccga tagaagggac cttccagtcc
    13561 cgattaaatc ccattcgcta acacaaaaca tcaaacatct cctatgagca ccatggttgt
    13621 gcgattaaaa cggcggtgaa atcgcacctg gaagctgctg ctaaagtaga gacaccccat
    13681 gacgtgtttc tacaataagt cttattttaa gagtttctca agaatcttcc cttttttatt
    13741 attttaaaaa tttactcttg acaataatat tgtcattatt ataatataat tgtctatatg
    13801 acaatatagt tgtcaaactt atgatgcgga ttacacaaat atcagttgga aaatcgagga
    13861 tgtaaatact aggttggaga aagctagtaa tgggacaggt gaaaacgagc aaaggaaata
    13921 aagtgctcca taaaagaagt ggcggcaatc aaaatacaaa gcctgctgct gtggcgttaa
    13981 gtaaggctta ttttggagat tcggcacttg cttcccttat tggagaggtg gtggctctct
    14041 ttcaccgcct tcggatagta tccgagcaga ttcaccggca gggcgagatg acatcgggaa
    14101 agcgaggtat attaggaagc cttgatcggt ttggaccgca gacggtgcca gagatggccc
    14161 gagctcggcc ggtctcccgt cagtacattc agacactcgt gaaccagctt atgcatgaag
    14221 ggcttgtcga atttatagaa aacccagccc ataagcgatc acattttgtg cgtttgactc
    14281 caaagggaaa ggaattactg gactcaatga ttcaacgtga gactgagtta ttgtcgcagt
    14341 tgaaactcga tataccggaa aaagcgctgc atgatggggc gggggttctt aaagccgtgc
    14401 gagagttgtt cgaaagcaaa cagtggaaag agcttttgaa ggaggatgga gtatgaaagt
    14461 tataaacaga atcttaaggc ctgctacatg ggttttctac ctagttatcg tttgtgagat
    14521 tttgtttatg ataagcccat ttgctttgta tttttattcc gcctacgggc cgacgctaaa
    14581 cttcttacat cattcgcctt atgccgcttg gctcacacag ttctttttac ctcatttctc
    14641 agagacgagt agcccattgc ttaacgcact gcccaagctt ggtggttttc tcattgttat
    14701 tggtgtgttg atgttttttg caggggcgat acccatttac tgggcaaaaa tcaggggtcg
    14761 aggtgcagtg accggcggac tctacaagtt cattaggcac ccccagtacg ttggattagc
    14821 tatcgtgggc ttaggaaccg tcctggtttg gcctcgcttc ctggtactta taacctacgt
    14881 caccatgctt tttctttacg acttgctagc tcgccttgaa gaagagcggt gtcttacgaa
    14941 attcggtgag acctatcgtg cttataaggc gcggacagga atgtaccttc ctcaagccct
    15001 atccactagt attccaagga ttctcccacc ttctgggccc aaaagggttt tagcaactat
    15061 cgggatatac atagtggtga tctcagctac tgtcacgttt gctttcagct tgcgtgatga
    15121 ttctcttaga aagatttctt cattctatac agatgatata gcagtgctgt caccagctac
    15181 ccttactgac gaagaactaa gggcggcttt taatatagcg atggcagagg atgaagttca
    15241 aaataaacta agaacagacg gtgcagcaaa gttgattgtc tacgttgttc ctcaggagtg
    15301 gtatattcct gatttgccta tagagtcgga gtacaagcct ggtggtcatc atgttcctca
    15361 agactttgac cggcggtatt acaaattact cttcacaaag ccacgcaccc acacaccaga
    15421 ggctactgca agaaatatag tcaaatccgc atacggtcga gatccaatcg ttctagttaa
    15481 ggttgatatt ctatcatcaa agctaatcgg aatcgaaaca ccaccgtctc atgtgtattg
    15541 gggagacatc ccaaccccta tgttttaagg aggtgctaaa aatgcatacg ataattgaga
    15601 tggttagttg atatttttgg tcaagccgag tgagtttaat ggtatttaaa aaaataaaaa
    15661 ggaggtaaat catgaacata gtgctaatca gtttaacggt aataaccggg attgttttaa
    15721 tatcagccct cggatttttt gggactttaa gaggcaaaga atctcgcgaa gaaaggaagg
    15781 aaagagctgt cgaatgggta tcttctgagt tggatcttac aaaggaacaa agatcaaaac
    15841 ttgtgcatat aagtgatgag ctgtcgaatt tagagaatga gatgaagaaa gaccgtgatg
    15901 aactcaaaga ggagtttatc aattcaattg cctctgatga gttagatcag gctaagattc
    15961 tggagtgggt aaaagaaaaa cagaaactag tggatgaatt tgctcccttt atcattgccg
    16021 aattagcaga tttccacaaa agcctaaatc cagaacaacg aaagaagctt gctgatgagt
    16081 taaataagca ctctcacgaa aacagatttg ggcatggcct ttaccatgga aatcactaag
    16141 gaatttagac tggtgtaaat gagtggaaat cccctctatc tcccctttaa gaaaggaaat
    16201 tagtccccct ttcacaaagg gggattcagg gatttaatat tatgaacagc cttctttaac
    16261 ctccctttca ctttttcctt tcccataatc tccataacct cgaaaatccc tgggcttaca
    16321 gttccaccgg tgagggcgac ccttacgggc tgggcgattt gaacgagttt tagtcctctt
    16381 ttctctataa tttctccgaa tatcttttgc agctcaggat ctgtaaactc atcaagagaa
    16441 gagaatctct ctataagggc ttcaaaaata ggaagtactt caggggttag aaacttcttt
    16501 cgagcagctt cttcgtattt aatttcatca atgaaaaaat aatacgatga gatggcaagt
    16561 tctacaagag ttttcgccct ttctctcagt atatttacga tttttatgag cctttcatcc
    16621 aatatgactt gaaatccttt cttttcgaga aatggaataa ctaattgtgc aatctcttcg
    16681 gcaggcttgt ttcttatata ccaaccgttt agccagagga gcttttctgg gttaaaaacc
    16741 gcaggagatt tcccaacatt ttcgagtgta aatttttcaa ttaactcttg tttagtgaag
    16801 atttcttggt ccccgtaaga ccagccaagg cgagtaagga aatttacaag cgcttcggga
    16861 aggtaaccca tttctctata tgcgacaaca gaagttgcac catgtctttt actcagtttt
    16921 gctttgtctg agccaagaat agttgaaaca tgagcgaatt taggaatttc ttgcgataag
    16981 gcctcgtata tcaaaacctg tttcggggtg tttgcaatat ggtcatctcc tcgtataacg
    17041 tgcgttatat ccattgtggc gtcatcaatc actacagcga agttatatgt aggaaatcca
    17101 tctgtcttct ggataatgaa atcgtcaatc tccttagcat caaatgtaat cgtccctctg
    17161 attacatcct gaacttctat acttcctatc tcaggtgtca taaggcggat agcataggat
    17221 ttgtgagatg gaggtgattt tagacctgta gcgagcctgt cgaacgtatt cctgcatatg
    17281 ccttcatatc tgtagacctt tccctgcttt tctgcttctt ttcttcttat ttcaagttct
    17341 tcttgagagc aatagcatct atatgctttt ccaacttgaa tgagtttttg agcgttgctt
    17401 tgatatatag aaagtctgtc agactgtcta aatggacctt catcccagtc gagtcctaac
    17461 catttcatag cgtctagtat ttcagcaatg gactcctcgg tagagcgggt acggtctgtg
    17521 tcttcaattc tcagaataaa ttttccacta tggtgtcttg caaatagcca gttgaagata
    17581 gcagttcgca cgccaccgat atgaagcgct cctgtagggc ttggtgcaaa tcttactctt
    17641 acactcataa ggtttaggtt aaattagagg ttgtggggag tcaaactaaa ggtgattcgg
    17701 gtttcgtggc tcgtggttcg ttatgaagta ttcacctgag ctaacccaaa cgattcgaat
    17761 cacgatacac gattcacgaa ccactagcta tttaaacatt tcatccaact ttccttctct
    17821 ttctagctct cgaagctgat cgtatccgcc tataagtttt tcattaatta gaattatagg
    17881 tacgctctgc cagccgagtt cttccatgac ctttgttttt atttcttcat tttctgtgag
    17941 atcaaattcc tcgtaattaa cacctttgct ttcaagaagt ctcttcgcag cacggcagta
    18001 tggacagtag gttgttgtat aaatcacaac ggtactcaat tttaattagc ctccattcca
    18061 acatttctaa tcttaatgat gcttggtttc tattttggtt tcaagaagag ttagtaattc
    18121 gtggctcgta tatcgaatat cgaacttaag ccgaatcaca aatccgaaca acgggtcacg
    18181 agtcactaac cacgattcat tatattttca tactggtatt ctctctccaa tatggtataa
    18241 atatttaaat tttcatgaag gtgatctgat gttcaggttt tttccaaagg aagaaaaatt
    18301 ttttgactac tttgatgatg cctcagaaaa gatgatccgc ggtataaaac ttcttaagga
    18361 aatggtgcag gatttgtcaa atgcagaaga gaaggccaag cagataaagg atgttgagca
    18421 tgaagccgac cacataaccc atgaaactgt atccaagttg cataaaacct ttataacccc
    18481 gatagaccga gaatatattt acgccctcat aacaaagatg gacgatattc tagaccttat
    18541 tgatgctacc tgcgagagga taattcttta caaaattaaa agctcaaccc ctgaggttat
    18601 agctttagtc aatactcttg agaaagccat agaggaagtc gcaaaaggta taagaggact
    18661 aaggaatcta gagaattctc gttcgatcct tgatatctgt attgaaatca atcggcttga
    18721 aaatgagggc gatagaacgt ataggaatgc tcagtcagta ctattcaatt caccctctga
    18781 tccaattgag ataatcaaat ggaaagacat atacgaaacg cttgaagaag ctattgatac
    18841 ctgtgaagat gtggcaaaca tcatagaggg aattgtccta gagaatgctt gatctccact
    18901 tatcttttat catctttgta attatagttg ctctcttctt tgactttact aacggcattc
    18961 acgacgcagc gaattctatc gccacaattg tttcaacacg ggtgctgacc ccaaaacaag
    19021 cagtcgcatg ggcagccttt tttaactttg tagcatttct aatttttggc acctcagtag
    19081 ctaaaacagt tggtaaaggt ttaattgaga ttagtacggt aactcctgcc gttgtttttg
    19141 ctggactgat aggcgctatt gggtggaatc tcaccacatg gtatctaggt ttaccaacta
    19201 gctcatctca tgccttaatt ggaggttacg ctggtgcggc tattgcaaaa tcaggcatag
    19261 gggtgatcat agcaagcggt tggtacaaaa cgctggtgtt tattattctg gcgccaacta
    19321 tcggtttact tcttggaatg attttaataa ttatagctac ctggctagtc tacaaggaaa
    19381 ctcctgcttt tgttaataaa tggtctaggc gattccagtt cctttctgca gctctttata
    19441 gcttgggtca tggtggtaat gatgcccaaa aaactatggg aatcatcaca gggctattgt
    19501 tttcaggtgg tttgattagc aaattcgcag ttcccctttg ggttgtactt agtgctcatg
    19561 ctgttatagg cttaggtact cttgtgggcg gctggaggat tgtaaaaaca atgggtcaaa
    19621 agattactaa actaagaccg attgacggat tgtgcgccga aactgcaagt gcagcaagca
    19681 tatttcttgc aacctatctc ggcataccag tcagcacaac tcatgtaatt acaggtgcga
    19741 tttcaggtgt aggcgcatca aaagggcttt ctactgtccg ttggggtata acattaagaa
    19801 ttgtatgggc ttggattttc accattcccg ctgcagcttt tattgcagga ctagcttata
    19861 atatattgaa tttttttact tagaaaccaa ctaattctta tttctccgaa ccatgacgta
    19921 tcaaaatcct taggtgttaa actgaaatcc actggttttt aaaatatctt ttgattaaac
    19981 taaaccctgt caaaaaaatg aacaacctcc ttacgatatt ggtcttcttt tctgtttttt
    20041 ttagttcctg ttccgttatt aaatttgctc gttactatgc ttccgctgat aattcttcaa
    20101 gcggcaaact cagtgggcag gtatatgagt ccaaagatac gatttatgag ata
//
LOCUS       DATDRL010000015         6105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1552315, whole genome shotgun sequence.
ACCESSION   DATDRL010000015 DATDRL010000000
VERSION     DATDRL010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552315"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VI935_00665"
     CDS             <1..717
                     /locus_tag="VI935_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HLE24141.1"
                     /translation="SLKPPTSQKTFGFYRAEILAAFLNSLLLFGISLGIIVEAYDRLR
                     IPTEVKSLEMTVVAFFGLGVNLLSAYLLSRIKSISLNIRGAIYHVISDALGSFGALVA
                     GLVMLKTKWYDADPIISILISILILRGAWKIFTESVHVLLEGTPRGIDLVSLELAIGS
                     HKGVLGVHDLHAWTLTQGFEALSAHLVIEDINLSEEIIEEIKRHLHDEFAIQHVTLQI
                     ETTKCNPLSVGCYETRTPSV"
     gene            complement(1074..2324)
                     /locus_tag="VI935_00670"
     CDS             complement(1074..2324)
                     /locus_tag="VI935_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HLE24142.1"
                     /translation="MAKSVKTAIKFIHLSDIHIGFETHGKLNPITGWNTRLEDVLGSL
                     DSVFETAIRESVDLVLIAGDVFHRENPHPTEETEFAKRISKLVSQGGTQVVIVLGNHD
                     YPTSFGKAAAVEIFPALNLEGVTIAKRPDIYPVITKNGQVQIACLPWARISTLLTKDE
                     YKSLYSEALQLEIEKRLIHIIRDLIKRIESTYPAVFLGHIALREATRSGTEMTTLLTQ
                     DPTIPRGELANSVFTYVALGHVHKFQNLNEGNKPPIVYSGSLERIDFSEEKEKKGFVM
                     GEIFKGEGGWECHFEFIETKARRFITIEAEEKDIYTSEEIINIIKKEDVRGAVVRLRL
                     RASKPEEVDEKRIKEALNEAYSIKIEKIFEKQEKVFRQSELSKSMDIVEALDKYISTK
                     PELKNIAEDMKRYAADLIQKSKEI"
     gene            complement(2579..3718)
                     /locus_tag="VI935_00675"
     CDS             complement(2579..3718)
                     /locus_tag="VI935_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HLE24143.1"
                     /translation="MKKYLFTPGPVPVPEEILLEMARPIIHHRTVEFENIFAEVREGL
                     KYIFQTKEEVIVLASSGTGAMEGAVANTLSRGDSALVVNGGKFGERWVKICQAYGIEV
                     HEIKVEWGEAVNPNQIKDALSKNPSIRAVLIQASESSTGVMHPTKEIAEITKERDDVI
                     LIVDGISAVGVFPQPLDAWGIDVLIGGSQKAFMLPPGLAFAALSKKAWKFNERSNLPK
                     FYFDFIRYLKDAKSNTTPWTPAITLIIGLASVLRNFKNEGLEKMYQRHARLAQATREA
                     IKAIGLELYAKHSPSTALTAAISPGDINAGKIISGLRDRFGMTLAGGQDQAKGKIFRI
                     SHMGFIDRADIIAVISALECVLRDLGYKFDFGAGSRSASDILGVE"
     gene            3856..4960
                     /gene="prfB"
                     /locus_tag="VI935_00680"
     CDS             join(3856..3924,3926..4960)
                     /gene="prfB"
                     /locus_tag="VI935_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139172183.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HLE24144.1"
                     /translation="MFAENKEKIEDLKKRIQILRDFLDLPKKKTRLEELETIISESNF
                     WTEPEKAQEVLKERAQLKSVIDDIEYLYHELEDAEVLAELSSEEEDEETAKETLAKIS
                     DIEDKIESLEFKRMFGDQDDEKNAIVSINSGAGGTEAQDWADMLLRMYLRYGERNGHQ
                     VEMLDYQPGDGAGIKSATFFVKGSYAYGYLKAESGVHRLVRISPFDANKRRHTSFASV
                     FVSPEIDEDIQVEIDEKDLRIDTFRAGGKGGQHVNKTDSAVRITHLPTGIVASCQNER
                     SQHQNRVVAMKVLRARLYELEKEKQKEKLEELHATKKDIAWGSQIRSYVLHPYRMIKD
                     HRTGYETGNVEPVLDGDLSEFINSYLLYKFANS"
     gene            5161..6024
                     /locus_tag="VI935_00685"
     CDS             5161..6024
                     /locus_tag="VI935_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HLE24145.1"
                     /translation="MRIGIIGNTKKKETFEITTELSKLLEKKGIEVYVEKELAAVIGH
                     KNSVPRSELLELVDLVLVFGGDGTFLGVARLVSKYDIPILGVNLGGLGFLTEITLDEL
                     YPMMEYILSGKYEIEKREMLSATIHRRNEIIGNYLVLNDVVVNKGAVARIIDLAIYIN
                     GFHVTTFKADGIILSTPTGSTAYSLSAGGPIVYPTLPLTIITPICPHTLTDRPLVVSN
                     ETTIRVKVLTDTLDIYLTLDGQVGVNLEVGDVVEVKKADSSVKVIKSPFRDYFTILKT
                     KLKWGERYGAV"
BASE COUNT         1747 a         1228 c         1239 g         1891 t
ORIGIN      
        1 tcacttaagc cacctacttc tcaaaaaacc tttggatttt atagagctga gatactagcc
       61 gctttcttaa atagtcttct tctctttggt atctcccttg gaattatagt tgaggcatat
      121 gacagattga gaattccaac cgaagtaaaa agccttgaga tgacagtggt agctttcttc
      181 ggtctcggcg ttaatcttct tagcgcatat cttctttcaa gaattaagag cattagctta
      241 aatattaggg gggccattta tcatgtcatt agtgatgcgc ttggttcctt tggcgcgctt
      301 gttgcaggtt tagtaatgct aaaaaccaaa tggtatgatg ccgacccgat aataagtatc
      361 ttgatttcga ttttgatact tagaggcgct tggaaaatat ttactgaatc tgttcatgtt
      421 cttcttgaag ggactccaag gggaatagac cttgtatctc ttgaattggc tatcggatct
      481 cataagggtg tactcggtgt acatgatctt catgcctgga ctctgaccca aggctttgaa
      541 gcactaagtg cacatcttgt gattgaagat attaacttga gtgaggaaat tattgaggaa
      601 attaaaaggc atctacacga cgaattcgca atccaacatg tgacactgca aatagaaact
      661 acaaaatgta atcctttaag tgtgggttgt tacgaaacga gaacaccgtc tgtgtaaggt
      721 tgagtattcc tttataagac ggtttatagt taattacaca tctaacttct cttgatcgta
      781 atccttaaat tatctttttt ccctgttgct gcaagctgag gcgaagcaac ccattctata
      841 tatatgagat tgcctcggga ctctgcccct cgcgatgaca ctcccgtgtt gtcattgcga
      901 acatgttctc catgctcagt gtaaaatctg tgaagcaatc tctatttttg gattgtttcg
      961 tcgctcctct ctccaggtga cggactgggg tttttattct cgccctactc agcgcttcac
     1021 ttcgctctag cggtaataaa gtaatcacgc ttgttttagg gtaaatatat aatttagatt
     1081 tcttttgatt tttgaataag gtctgccgca taccttttca tatcttcggc aatattttta
     1141 agttccggtt tagtgctgat atatttatcc agagcttcca caatatccat agattttgat
     1201 aattctgatt gcctgaatac tttttcttgc ttttcaaaga ttttttctat ttttattgaa
     1261 taagcctcat ttagagcttc cttaattctt ttttcatcga cttcttctgg ttttgatgct
     1321 cttaacctta atctaaccac ggcacctcta acatcctctt ttttaattat gttaatgatt
     1381 tcttcgcttg tatatatatc tttctcttca gcttcgatgg taataaacct tcttgctttc
     1441 gtttctataa attcaaagtg acattcccaa ccgccttcgc ctttgaatat ttcacccata
     1501 acaaatccct ttttttcttt ttcttctgaa aagtcaatcc tttctagact acctgaataa
     1561 actattggag gtttattgcc ctcattcaaa ttctgaaatt tatgtacatg accgagagct
     1621 acataagtaa acacagagtt tgcaagctca ccccttggga tggttgggtc ttgtgtaaga
     1681 agagtggtca tttctgtacc tgaacgcgtt gcctctctta aagcaatatg tcctagaaaa
     1741 acagcaggat atgtgctttc tattctctta attaagtcac gtattatatg tattagtctc
     1801 ttttcgatct ccaactggag cgcctccgaa taaagcgatt tgtattcgtc ctttgttagt
     1861 aacgtgctaa ttcttgccca aggaaggcag gcaatctgaa cttgtccatt ttttgtaatt
     1921 acagggtaaa tatcgggtct ttttgcgatt gttactcctt caagattgag agcaggaaat
     1981 atttcaacag cagcggcttt tccaaagctt gttggatagt cgtggttgcc aagcactata
     2041 acaacctgtg ttccaccctg agagacgagt tttgatattc gctttgcaaa ctcagtctct
     2101 tccgtaggat gggggttttc tctatggaaa acgtcaccag ctattaggac taaatctact
     2161 gactctcgaa tagcagtttc aaaaactgaa tctagggaac ctagtacatc ttcaagcctt
     2221 gtgttccatc ccgttatggg attaagcttt ccatgagttt caaacccaat atgaatgtcg
     2281 gatagatgga tgaatttaat tgcggttttc actgattttg ccacagagac cacagagttc
     2341 acggagaggg attctttttt tcattcataa actctgtgtt ctccttgctg tcttaaggtc
     2401 taggaatttc ttatgaactc ccgttaagca tgtgttactt ttgtcatgcc cgaatgttcc
     2461 ccgctaaaga catgcgggga caagatttaa cgggcatcca taatgtgatt gaaatgttgg
     2521 attcccgctt aatacatgcg ggaatgacaa ccttagtaca taatattact tatgaacctt
     2581 attccactcc caaaatgtca gaggcgcttc ttgacccggc tccaaaatcg aatttatatc
     2641 caagatctct gagaacgcat tcgagagccg agataacggc aatgatatca gccctatcaa
     2701 tgaaccccat atgagagatt ctaaagatct ttccttttgc ctggtcctgt cctccagcaa
     2761 gggtcattcc aaatctgtct ctcagtccgc ttataatctt tcctgcattt atatcaccgg
     2821 gagaaatagc tgctgtaaga gcggtgctcg gcgaatgctt cgcatagagt tcaagcccta
     2881 ttgcctttat cgcttctctt gtggcttgag caagccttgc gtggcgctga tacattttct
     2941 caagaccttc atttttaaag tttcttagaa ctgaagcaag acctatgata agagttatag
     3001 ctggtgtcca tggtgtggtg ttactctttg catctttgag ataccttatg aaatcaaaat
     3061 agaacttggg cagattcgat ctttcattaa atttccacgc ctttttgctc aaagcggcaa
     3121 aagcgagtcc tggaggaagc ataaatgcct tttgagagcc tccaataaga acatctatac
     3181 cccatgcatc aaggggttgt gggaatacac caactgcgct aattccatct actattagaa
     3241 taacgtcatc cctttctttt gtaatttcag caatctcttt tgtcggatgc ataactcctg
     3301 ttgacgattc gcttgcctga attagaactg cacggattga agggttctta ctaagggcgt
     3361 cttttatctg attaggatta acagcctcac cccattcaac ctttatttca tggacttcaa
     3421 tgccataagc ctgacagatt tttacccatc tctcgccgaa ctttccacca tttacaacaa
     3481 gagcagaatc tccgcgagaa agtgtattcg caactgcccc ttccattgcc cctgtgcctg
     3541 aagaagcaag tacaattacc tcttcttttg tttgaaatat gtacttgagt ccttccctaa
     3601 cctcggcaaa tatgttttcg aactctacag tcctgtgatg tattataggc cttgccatct
     3661 caaggagtat ttcctctgga acaggaacag gaccaggtgt aaagaggtac tttttcacgc
     3721 taaaaattct cctttttgcc tacaaataga tttaagaaac tctaccttac actggggata
     3781 attcaactaa tggcttgttt ttcttacatt cagatcttat attaatttat atatgaacat
     3841 ggaggattta atttaatgtt tgcagaaaat aaagagaaaa ttgaagattt gaaaaagaga
     3901 atacaaatcc taagggactt tctttgacct ccctaagaag aaaacacgcc tagaagaatt
     3961 agaaacaatc ataagtgagt caaacttctg gacagaacct gaaaaggctc aagaagtgct
     4021 aaaagaacga gctcaattaa aaagcgttat tgacgacata gagtatcttt accatgagct
     4081 tgaggatgcg gaggtgctgg cggagctatc ttctgaagag gaggatgaag aaacggcaaa
     4141 agagacacta gcaaaaatct ctgatatcga agacaaaatc gagagcttgg agtttaaacg
     4201 aatgtttggt gatcaggatg acgaaaagaa tgcaatagtc tctatcaatt caggcgctgg
     4261 tggaaccgag gctcaagatt gggctgatat gcttcttaga atgtatctca ggtatgggga
     4321 aagaaacgga caccaagtag aaatgcttga ttatcaaccg ggagatggag ctggaatcaa
     4381 aagcgctaca ttttttgtaa aaggttctta tgcctatggg tatctcaaag cagagagtgg
     4441 cgtacatcgt ctagtccgta tttctccatt tgatgcgaac aagagaaggc acacctcttt
     4501 tgcatccgta ttcgtttcac ctgagataga tgaagatatt caggtagaga ttgatgagaa
     4561 ggatttaagg attgatacat ttcgagccgg tggcaagggt ggccaacatg taaataaaac
     4621 cgactcagct gtgaggatta cacatcttcc gaccgggata gtggctagtt gtcagaatga
     4681 gcgttctcag catcaaaatc gtgttgtagc aatgaaggtt cttcgtgcaa ggctatacga
     4741 acttgaaaaa gaaaaacaaa aagagaagct cgaggagcta catgcaacaa agaaggacat
     4801 agcatggggt agtcagattc gttcctatgt gctacatcca tataggatga tcaaggatca
     4861 tagaactgga tacgagacgg ggaacgttga gccggttctt gatggagacc ttagtgaatt
     4921 tattaacagc tacctgcttt acaaatttgc taattcatag ttgaccccat agtacctgat
     4981 gcgtgattcg tgaatcgtgt atggtggttc gaaccttcaa tcacgggtca cgaaacccga
     5041 gttacgagcc acgaagaccc gtatttgatt ctttaacccg ccaacgtata ttatattcac
     5101 actccttaga ctgcaaaaac tccataaatg gcagccgatt gagtgggagg aaggagagtt
     5161 ttgaggattg gtattatcgg caataccaag aaaaaagaaa cctttgagat tacaacagaa
     5221 ctatccaagt tgcttgaaaa gaagggcatt gaggtttatg ttgagaaaga acttgcagcg
     5281 gtaatagggc ataaaaactc tgttccacga agcgaactcc ttgaacttgt agatctggtt
     5341 ttagtttttg gtggagatgg gacttttctt ggagttgccc gccttgtctc aaaatacgat
     5401 attccgattt taggagtaaa ccttggagga ctgggcttcc tcaccgagat taccctagat
     5461 gagctctatc cgatgatgga gtatatcctt tcaggcaaat acgaaattga aaaaagagaa
     5521 atgctttcag caaccattca taggaggaat gaaataatag ggaattatct ggttcttaac
     5581 gacgttgtag ttaacaaggg agcagttgca agaataattg atcttgcaat atacataaac
     5641 ggttttcatg taactacctt taaggcagac ggaataatcc tttcaacccc tacaggttct
     5701 actgcttatt ccctttctgc tggaggaccc attgtttatc caacgcttcc tcttactata
     5761 attaccccga tttgtcctca tacccttacc gaccgccctc tagttgtttc caatgaaact
     5821 acaatcaggg tcaaagtcct tacagataca ctagatattt acttgacact agatggccag
     5881 gttggagtta acttggaggt tggagatgtg gttgaagtaa aaaaagctga ttcttctgtg
     5941 aaggtgatta aatccccatt cagggattat tttacgattc ttaaaaccaa gcttaagtgg
     6001 ggagaaagat atggagcggt ctagggatga acagtttctc cacgattggg taattagcaa
     6061 gataaaagag aaatactcaa gactctacaa agaagtccat acaaa
//
LOCUS       DATDRL010000016        14869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2609120, whole genome shotgun sequence.
ACCESSION   DATDRL010000016 DATDRL010000000
VERSION     DATDRL010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14869
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2609120"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1722)
                     /locus_tag="VI935_00690"
     CDS             complement(<1..1722)
                     /locus_tag="VI935_00690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HLE24146.1"
                     /translation="MTEELLKILEALEKPLRFTSKNDFLNIGKVKDLDQLVSDLTLKA
                     LTLSLSSGELRVIQTLRKSFSVYKELEPISKKKLIEESLNAIVDLKNYKYSDYSYATQ
                     TTHSIVKEASEKKDLSKIPIQYLKGVGPRISNILAKKGIKSVEDALYYFPRRYEDRRH
                     IKKISELQPFKRETVMGKIILTGKVRTKRRELFQVVLSDGTGRLNLVWFQYNEKYLRA
                     VYKKEATVILSGEITVDRYTDSLQIIHPRPEDTEIIDSEENLEKDFLNFNRIVPIYPL
                     TEGIKQRRLRSIMKTVVDAYCPHVIDYVPEAIKRRRHILNLGDALSRAHFSSASDRLV
                     DLFDRDSVYGSIPHRTVSFNEFFFMELGLALRKRDVTKLPGIAFNPTGRLSGRLLSNL
                     PFSLTSAQERVVAEIERDMRADSPMNRLLQGDVGSGKTIVALLSMLKAVDSGYQAAIM
                     APTEILAEQHLRSIIQFTRTAGVKVVLLTSGLSKKERYTYYEAIKTGEADIAIGTHAL
                     IEEAVEFKNLGFIVIDEQHRFGVIQRAELRSKGKNPDVLVMTATPIPRTLAMTVYGDL
                     DVSVIDEM"
     gene            1933..2181
                     /locus_tag="VI935_00695"
     CDS             1933..2181
                     /locus_tag="VI935_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24147.1"
                     /translation="MAVLKATFTIPEELLRRLEKVPAGKRSLLVAEAIKRELNRKSLA
                     KALKKLKRRTVWKEKDHPDLLSPEDFSRYRPAKSRVTG"
     gene            2178..2567
                     /locus_tag="VI935_00700"
     CDS             2178..2567
                     /locus_tag="VI935_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLE24148.1"
                     /translation="MSYLLDTNFVIGLLRGASSYWDYLDKLVDETVPAVSAVTRSEVF
                     AGCHPHEERATTNLLDRFEAIAIQSSIADLAGRYVYQYRKRGFTIHLEDALIGSTVVL
                     EGLVLVTRNVDHFPMLKFRHNLVRFPD"
     gene            complement(2618..4789)
                     /gene="topA"
                     /locus_tag="VI935_00705"
     CDS             complement(2618..4789)
                     /gene="topA"
                     /locus_tag="VI935_00705"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HLE24149.1"
                     /translation="MSKSLVIVESPAKAKTIKKYLGKDFSVEASSGHLIDLPNNKLGV
                     NIEKNFKPEYVVIRDKKKFLDQLEKASKKADNIYLASDPDREGEAIAWHIVDRLKIRD
                     KAYRVLIYEITEKAVLEAIRKPSRLSRDMFEAQQARRILDRLVGYQVSPLLWKKVRRG
                     LSAGRVQSVALRLVVEREREVENFKPDEYWTIEAELKQLLDDAISFVAVLSKYDGEKV
                     DIKTEEAANRAVEALRNETYSVISVEKKERSRSAPPPFITSTLQQEASRKLRFPVKKT
                     MTIAQKLYEGVELGDEGPSGLITYMRTDSVRVSEHAINEARVYIKEAYGADFLPEDPN
                     IFKVKKSAQDAHEAIRPTTVINSPDSLKIYLSEDEFELYKLIWQRFIACQMNPALYDQ
                     TTVEIEAGRGLFRATGSVIKFPGFTVVYMEGKEEEEEAKEKEEEKRLPDLSEGEDLKL
                     IALEGKQHFTQPPPRFTESALVKELEERGIGRPSTYATILSTIQDREYVIREKNRLKP
                     TLLGCAITDLLIEGFPEVMDVQFTAEMEESLDEVEEGRVNWVELLNGFYKGFGKRLKQ
                     AEVSMRSLRREGMPTELDCEQCGGQMIIKWGKRGEFLSCSKYPECKNAKQFEYGEDGE
                     IRIKQKKEPEIRHDILCDLCGKPMVVRTSRYGKFLGCTGYPECKGIKRFKDIQDEEAG
                     KVIESGESTEENKKESKRKSFRKSPAKKKADGRPQAKGYQS"
     gene            complement(5326..7431)
                     /locus_tag="VI935_00710"
     CDS             complement(5326..7431)
                     /locus_tag="VI935_00710"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HLE24150.1"
                     /translation="MLLVLVLGISVISLGFAGYLAKYVLNSDTGTSAMQDISNAIKEG
                     AEAFLKRQYSTIIMLSIVLAVLIFILYWFAKGDRDMAWRTTVAFLFGAGCSALAGYIG
                     MWISIRSNIRTASAVRSSLDRALKISLRGGAVSGITIVAMSLLGVGILFTLYGGSADD
                     PEVVKRVPFLIVGYAFGASFVALFAQLGGGIYTKAADVGADLVGKVEAGIPEDDPRNP
                     AVIADLVGDNVGDCAGRGADLFESTAAENIGAMILGVALFPIFGIKGILFPLVARAFG
                     LIASMVGVAIVGLKNEKEDPMSALNRGYYVACILAAVGFYIATYLLLDNNFWLFAAGV
                     IGIATSIVFVFITQYYTEYRYRPVKFIAEASQTGPATNIIAGLSVGLECTALPVIAIS
                     AALVGAYQCGIYGLKEFSDISTTSAGLYGTAIATMGMLGTAAYILAMDTFGPITDNAG
                     GIVEMSKQPEDIRVKTDRLDAVGNTTKALTKGYAIGSAALAAFLLFSAYLDEVTHITG
                     KAFHSVDITKVEVFVGGLLGAMLVFLFSALAIRAVARAAYYIIGDVRAQFKENPGILE
                     GTAKPNYARCVDIVTKGALKEMVPPGILAVAMPIVVGLVFKQFGLGAETVAAFLMVGT
                     ISGILVATLMNNGGGAWDNAKKFIETGMYGGKRSETHKAAVVGDTVGDPLKDTAGPSI
                     HVLIKLLSTITLVLAPLFI"
     gene            8374..12300
                     /locus_tag="VI935_00715"
     CDS             8374..12300
                     /locus_tag="VI935_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017315797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLE24151.1"
                     /translation="MYNDLIKTYQKITRIFRDLIIFTIIAIFTFVLAIVFIFDAFEKL
                     VELTRKYEIVEIDELITLLAGLSFAFSIFSFLRWKELRHETIKRKQVEEELNNRNKDL
                     EILNTITQAVHQSFDLKEVYRIALDQVIELDNVDLACIYLVYEAKNVAVMQDQRNFPE
                     EFLQRAGVIPYPKGATWKVISTGKISNVKNAQTDPDVGPAGRDLGFRSMLGIPINLEG
                     KTIGVIWLLSYKEYLFTTREEGLLVSIGNHIATAIAKAKLYGELSKKSRYETIISAVT
                     RSVHQSIELQEVLENAVDAMSKNISGVEHVAIHLVEGEEAVIKAHRGHPDWFVERVRR
                     IPYPKGFTWKTIIEGKPRYCPDVDQDTVIGPAGREVGTKSYLSMPIRDEVKTVGCINV
                     HSFRKNSFDEEEIKLLEIVAQQIETAIKNAQHAEALRESEERFRNMADTAPVMIWMSA
                     PDKLCNYFNKVWLEFTGRTVEQEMGNGWTEGVHPDDLQRCLDTYFTAFDDRRDFKMDY
                     RLRRFDGEYRWIIDAGTPRFMHDGGFAGYIGSAIDVAERKHIEDELKTLNAIMEAVHR
                     SSDLKEVFNIALDKVRELTDIDIVGIYLVDEGTNEAMLEAHRGYPDIYIERAGRIPYP
                     KGVTWKVINSGENYVVQDVSTDPYVGPVGKESRFQSFMSVPIKMVDKVIGAIHFHSYK
                     KNKFGKREIELFSSIGTQIAIAVAKAKQREDLQLANEDLSVLNAIATSIHKSLNLQEL
                     YNIALDTVLEITAFDIIMIYLVDENTNEAVLQAYRGLTEDYIKRAGRIPYPKGVTWKV
                     INSGELTLIDDIQKDPNLGPAGRVLGHHTMLIVPIKQEEKAIGIIGFASLKVLELSSR
                     EISVLNAIGSQIGTAIVQARLYEKSQNQSEELRALYEDLDRRNRDLEILNTITQAVHQ
                     SFNLDEVYNVALDAVISLENVDMAIIYLVDDDRKEAIVQAHRNFPEDYLQKARRIPYP
                     KGITWKVINTGEMINIEDAQKDPEIGPAGRELGHHSLLGVPILLEGKAAGVIWFLSYK
                     ERKFAEREANLLSTLGNQISIAIAKARLYRDLKKTNEKLKELDVMKDEFVSMSSHELR
                     TPMSAIAGYVSMLFEGDFGELPSEAKDALTDVNTATQRLISLVNDILDVSRIEQGRLE
                     LKILEFDLGEICQEVLDNLTPISQEKGIKLAYQKSAEVVRPLVSADMNKVMQVLNNLV
                     GNALKFTQKGEVIITHRFDGNLIITDVIDTGVGISSQDQQFLFQKFQQLDKTLSGNRL
                     GGTGLGLYFSRELVRAMDGDLWLERSEIGEGSTFSFSLSRAKAMGS"
     gene            12357..12728
                     /locus_tag="VI935_00720"
     CDS             12357..12728
                     /locus_tag="VI935_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLE24152.1"
                     /translation="MPKLLLVEDDEILIRMYRTKLERENFEVDTAQDGEECLTKIREF
                     KPDIVLLDIVMPKLNGLDVLKRVKAQPELKGIPVVILTNAPVHIAAKECLQLGALGYI
                     IKSDNTPSEVVKKVREFLNMG"
     gene            12732..13334
                     /locus_tag="VI935_00725"
     CDS             12732..13334
                     /locus_tag="VI935_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLE24153.1"
                     /translation="MADSNKSKVLMIEDDPVLQRMYISKLTEGGYEVDLARDGAEGLH
                     KALNTDPDLVLLDLMLPKVDGLQILAEMRKNPKTRKTPVIVLSNLAQADDATKALQLG
                     ADDYVVKLNTPPSSVLKKVQMLLSQRKARDAIYTSRYHIKIDTTQADAPQLVRDFGLI
                     FFCAHCRTQQILELMPDYNSTGSGHRFVAQFVCPICKSVY"
     gene            13460..13789
                     /gene="trxA"
                     /locus_tag="VI935_00730"
     CDS             13460..13789
                     /gene="trxA"
                     /locus_tag="VI935_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HLE24154.1"
                     /translation="MASAGIVVLSDSTFEGEVLKSESPVLVDFWAPWCGPCRILAPVV
                     EEIANSYTGRIKVGKLNVDDNQETTMQYSIRSIPTLILFKNGKALDQIIGAVPKSEIE
                     KMVKKAL"
     gene            complement(14139..14522)
                     /gene="speD"
                     /locus_tag="VI935_00735"
     CDS             complement(14139..14522)
                     /gene="speD"
                     /locus_tag="VI935_00735"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HLE24155.1"
                     /translation="MRALGRHLLAEYLGCDPKVLNDLERVKQCMLEAASHCGATVLES
                     SFRYFTPQGVSGVVVIAESHLAIHTWPEYEYAAVDIFTCGTAVDPWAAFHHLKLSLDS
                     KECCVREIVRGDVSHLEGWLSRKTV"
     gene            complement(14686..>14869)
                     /locus_tag="VI935_00740"
     CDS             complement(14686..>14869)
                     /locus_tag="VI935_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HLE24156.1"
                     /translation="IEDISHLPAILVERLRHYFITYKLELGTQSQMTIEKIYNCEHAL
                     QVVEAAMEDYYEEYGA"
BASE COUNT         4419 a         3154 c         2998 g         4298 t
ORIGIN      
        1 catctcatct ataaccgaaa catcaagatc accataaaca gtcatagcaa gtgtcctagg
       61 tataggggtc gcagtcataa caagcacatc aggattttta cccttgcttc ttaactccgc
      121 cctttgtata acgccaaatc tatgttgctc gtcaattaca ataaagccaa gatttttaaa
      181 ctccactgct tcctcgataa gcgcatgtgt gcctatagca atatccgctt ccccagtctt
      241 aatagcctca taataggtat atctttcctt tttacttaaa ccactcgtca aaagtacgac
      301 tttgacacca gcggttctag taaattgtat gattgacctc aaatgctgct cagcaaggat
      361 ttctgtaggc gccattatag cagcctggta tccactatca accgccttca gcattgatag
      421 cagtgcaact attgtttttc cactcccgac atctccttga agaagtctat tcatcgggct
      481 atcagccctc atatctcttt ctatttcagc aactacccgc tcctgcgctg aagtcaaact
      541 gaaaggaaga ttgctgagaa gtcttccaga aagccttcca gtaggattaa aagctattcc
      601 aggcaatttg gtaacatctc tttttcttaa tgcaagccca agttccatga aaaagaactc
      661 gttaaatgaa accgttctat gggggattga accataaacc gagtccctat caaagagatc
      721 tactaatcgg tcagaagcac ttgaaaaatg ggccctcgat aaagcgtcgc ctaggtttag
      781 aatatgcctc cttcgtttta tcgcttctgg aacatagtca attacatgag gacaataagc
      841 atctactacc gttttcatta tgctcctaag gcgtctttgc ttaatgccct ccgttagagg
      901 atagatcgga acgatacgat taaagttaag aaaatctttc tcgaggtttt cttcactatc
      961 aataatctcg gtgtcctcag gccttggatg aattatctgg agggaatcag tatatctatc
     1021 caccgttatc tcaccgctta atattactgt cgcttccttt ttatagacag cacgtaaata
     1081 tttttcatta tattgaaacc aaacaaggtt aagcctgcca gtcccatcag ataaaaccac
     1141 ttgaaagagc tctcttcgtt ttgtccttac cttccctgtt aggattatct ttcccataac
     1201 tgtttcacgc ttaaaaggtt ggagttccga aattttctta atatgtcttc tatcttcata
     1261 ccttcttgga aaatagtaga gggcatcttc tacactctta attccctttt ttgctaaaat
     1321 attggaaatc ctggggccaa cgcctttcaa gtactgtatc ggaattttag aaaggtcttt
     1381 tttctctgac gcttctttca caatcgaatg ggttgtttgg gtagcataag aatagtctga
     1441 atatttataa ttttttaaat ctactatagc gtttaatgat tcttctatta attttttctt
     1501 gctaattggt tctaattctt tataaacaga gaatgacttt ctaagagttt ggattaccct
     1561 aagctcgcca ctagataaac taagagtaag ggcttttaaa gttaaatcag ataccaactg
     1621 gtcaaggtct tttactttac cgatattaag aaaatcattt ttagaagtaa acctaagcgg
     1681 tttctctagt gcctctagta tcttcagaag ttcctcagtc attcataata atcttactgt
     1741 ttcctaccta catatttcaa gttgactccg ttcagtgcta aaataatggt acttgccctt
     1801 tatgatctca ctaacccgat ctagaagttt cttaggacgt tattcaataa tttcgctgca
     1861 tgtacattga cttgcgttga ctatctaatt tgtatcagta tacttatacc aataagtatt
     1921 tggggaaaca aaatggctgt tctaaaagca acttttacta ttcctgagga actgctaaga
     1981 aggctagaaa aggtccctgc tggtaaacgg agtttactgg tagctgaggc cattaagcgg
     2041 gaacttaatc gtaaatcatt ggccaaggct ttgaagaagc taaaacgcag gactgtatgg
     2101 aaggaaaagg accatcctga tcttctatcc ccagaggact ttagtcgcta tcgacctgcc
     2161 aagagtcgtg tgacaggatg agctatcttc ttgatacaaa ttttgttatc ggtcttctac
     2221 gtggtgcaag ctcctactgg gattatttag acaaattagt agatgaaacg gtacctgccg
     2281 tctcagcagt tactcgttca gaggttttcg caggctgtca ccctcatgaa gagagagcaa
     2341 caacaaatct ccttgatcgc ttcgaggcca tcgcgattca atcctccatt gctgatcttg
     2401 ctggaaggta cgtctatcag taccggaaaa gaggatttac tatacactta gaagacgctt
     2461 taattgggtc tactgtggtt ttggaagggc tcgtattagt aacccggaat gtggaccact
     2521 tccctatgct taagtttagg cacaaccttg tccggtttcc cgattgatta ttaacttaca
     2581 aacttggagc tctttggaaa atgggtcatg aatggggtca tgattggtat ccttttgctt
     2641 gcggccttcc atcagctttc tttttagcag gacttttccg aaatgacttt ctttttgact
     2701 ctttcttatt ttcttcagtg ctctccccgc tctctattac tttacccgct tcttcatcct
     2761 gaatatcttt aaaacgcttt atacctttac attcaggata acctgtacaa ccgaggaact
     2821 tcccatacct gcttgttctt actaccatag gtttcccgca tagatcacag aggatatcgt
     2881 gcctgatttc gggctccttc ttctgtttaa tccttatttc accgtcttca ccatattcaa
     2941 actgttttgc attcttacat tcgggatatt tcgagcagga taaaaactct cctctttttc
     3001 cccactttat tatcatctgc ccaccgcact gctcacaatc taactctgta ggcataccct
     3061 cgcgcctaag actcctcata gaaacttccg cctgtttgag cctctttccg aatcccttat
     3121 agaagccatt taaaagctcc acccagttga cccttccttc ttccacctca tcaaggcttt
     3181 cctccatctc tgctgtaaac tgcacatcca taacctcagg aaaaccctct ataagaagat
     3241 cggttatagc acagccgagt aatgtgggtt ttaacctatt cttttccctt attacatact
     3301 ccctgtcctg aatcgtagat aatatcgttg cataagttga agggcgtcct attcccctct
     3361 cttctaactc ctttacaagc gcactttctg tgaaccttgg aggtggttgt gtaaagtgct
     3421 gttttccttc aagagcaatg agttttaagt cctcaccctc acttaggtca ggaagccttt
     3481 tctcttcctc tttttccttt gcttcctcct cctcttcctt tccttccata tacactacag
     3541 tgaatcctgg gaatttaatt actgaacctg ttgcacgaaa taagcccctt ccagcttcaa
     3601 tctcaaccgt tgtctggtca tagagggctg gattcatctg acaggcaata aacctttgcc
     3661 aaataagctt ataaagttcg aactcgtcct ctgataaata gatttttaaa gaatccggag
     3721 aattaattac cgtggtgggt cttatagctt catgcgcatc ctgggcggat tttttcactt
     3781 taaaaatatt agggtcttct ggaagaaaat cagctccgta agcctcttta atatagaccc
     3841 ttgcctcatt aatcgcatgc tctgaaaccc taactgaatc tgttctcata tacgtgataa
     3901 gccctgaagg accttcatca cctagctcca caccctcgta aagcttttga gcaatagtca
     3961 tagttttttt cacaggaaac ctaagctttc tcgatgcctc ttgctggagt gtgcttgtta
     4021 taaatggggg tggtgcagac ctcgatctct cttttttctc aaccgatata acactgtatg
     4081 tctcatttct taaggcctct acagctctat ttgctgcttc ctccgttttg atatctactt
     4141 tctctccgtc atatttagaa agtacggcaa caaatgaaat agcatcatcc aaaagttgtt
     4201 ttaactcagc ttcaatagtc cagtactcat ccggtttaaa attctctact tccctttctc
     4261 tttcaacgac gagtcttaaa gcaacactct gtacccttcc cgcactaaga cctcttctta
     4321 cctttttcca gagaagcggg cttacctgat aacccacgag acggtcgagt attcgccttg
     4381 cctgttgcgc ttcaaacatg tcacggctta gcctactcgg ctttcttatc gcctcaagaa
     4441 cagccttctc cgtaatttca taaatcagga ctctataagc tttatctctt atctttagcc
     4501 tatccactat atgccaagct atcgcttccc cttccctatc agggtcagaa gcaagatata
     4561 tgttatcggc tttcttcgaa gccttttcaa gctggtcgag gaattttttc ttatccctaa
     4621 tcacaacata ttcaggttta aaattcttct cgatatttac gccgagtttg ttattgggaa
     4681 gatcaatcaa atgaccagat gaagcctcga cgctgaaatc ctttcctaga tactttttta
     4741 tagtttttgc tttagcagga gactcaacaa taactagaga cttggacatt taacctccac
     4801 tatttcaact ttgttataaa gttaataaca ttcagaatac tttcaataag tctattaatt
     4861 ttttgaaaca ttattacctg aaattattct ctgtcaatca gctaattgtt aattgagtcg
     4921 ctaactaata gtaattgcgt acttttattg gttaaatatc ttttaaaaat gagtgtggag
     4981 gaaatttaga gtgggaatat atattttctt aattatctag atcattgcat ttgaaaatca
     5041 acaatcctgc agcaaggagg ctgtccgata aatgcaaaaa tcggcattct tggggtggca
     5101 aaacccctcc cgggttgccg accgttgatc ctggggcatt ctgaggggtc aaatttttac
     5161 catccataat tgtcggacag cctctaagct acatggaatt accaaggtaa agaaagagtt
     5221 atgtggatat ttaaggattg agcaatattt cgataattca atttgacaag aggaagagag
     5281 gcatcttaga tgtccctctt cctcctcagt gttatttcaa ccaagttaaa taaacaaggg
     5341 tgcgagaaca agtgtaattg tagagagaag tttaattaga acgtggattg atggtccagc
     5401 agtgtcctta agtgggtcac ctaccgtgtc tcccactact gcagctttat gagtttcgga
     5461 cctctttcca ccgtacattc cggtttctat gaacttcttt gcgttatccc atgcgcctcc
     5521 cccattattc ataagggttg caacaagaat tccactgatt gttccaacca taagaaacgc
     5581 agctactgtt tcagccccga gaccaaattg cttaaacact agccccacca ctataggcat
     5641 ggcaacggca agaatcccag gaggaaccat ttccttgagt gccccttttg taacgatgtc
     5701 aacacacctt gcataatttg gtttcgctgt tccctcaaga atgccggggt tttccttaaa
     5761 ctgagcgcgg acatcaccga ttatataata agccgcacgt gcaacggctc taattgcaag
     5821 agcactgaag agaaacacaa gcatcgctcc cagaagacct cctacgaaca cctcaacctt
     5881 ggtaatatca accgagtgga aagccttacc tgtgatatgg gttacttcat cgagataagc
     5941 tgagaaaagt aggaatgctg cgagcgccgc ggagccgatt gcatatcctt ttgtaagagc
     6001 tttggttgtg tttcctacag catcgagcct gtctgtttta accctgatgt cctcaggctg
     6061 tttgctcatc tcgacaatac cacctgcgtt atcagtgatc ggtccgaatg tatccatagc
     6121 aagaatgtat gcggctgttc ctagcattcc catcgtagcg attgcagtgc cgtaaagccc
     6181 agcactagtc gtagagatat cactgaactc ctttagccca taaatcccgc attgatatgc
     6241 cccaaccaat gcagccgaga ttgcaatcac agggagtgct gtacactcaa gcccaactga
     6301 aagcccggct atgatgtttg tggcaggacc cgtttgagag gcttcggcaa taaacttcac
     6361 aggacgatac ctgtactccg tgtaatactg cgtgataaag acaaatacga tgcttgttgc
     6421 aattccgata acaccagcag caaatagcca gaaattattg tcgagtaaca aataagtagc
     6481 tatataaaaa cctacagcgg caaggatgca agcaacatag tacccgcgat ttaatgcaga
     6541 catcgggtct tccttttcat tttttaaccc cactatcgca acccccacca tactggcaat
     6601 aagcccaaag gcacgtgcaa caagagggaa tagaatcccc tttataccaa agatagggaa
     6661 aagtgcgact cccagaatca tagccccaat gttttcagcc gctgtggatt caaagaggtc
     6721 tgcaccacga cccgcacagt cgccgacatt atcacccacg aggtctgcaa taaccgcagg
     6781 gtttctgggg tcatcttccg ggattccagc ttctaccttt cctacaaggt ctgctccaac
     6841 gtcggcggct ttagtgtaga ttcccccacc aagctgagcg aaaagggcaa cgaaacttgc
     6901 cccaaatgca tatccgacta tgagaaaggg aacccttttc acaacctccg ggtcatctgc
     6961 tgagccaccg tagagggtaa aaaggattcc tactccaaga agactcatag caacgattgt
     7021 aattcctgaa actgctcccc ctcgaaggga tattttaaga gccctatcca ggctcgatcg
     7081 aacggcactc gccgtgcgga tgtttgacct gattgaaatc cacattccaa tgtagcccgc
     7141 aagagccgag caccccgcgc cgaaaagaaa agctactgtg gtgcgccatg ccatatctcg
     7201 atcacccttt gcaaaccaat atagtataaa gataagaacg gcaagaacga tgcttagcat
     7261 tattatggtt gagtactgcc tttttagaaa cgcctcagca ccctctttga tagcgttgct
     7321 tatgtcctgc atcgccgaag ttcctgtatc actgttaagc acgtacttgg ctagataccc
     7381 agcgaaacct agtgatataa cactgatgcc tagaacaagc acaaggagca tgtggtcaaa
     7441 ttccattttt gattcctcct ttctattatt taaagaacag gcctaatttt gttggaatat
     7501 taggaaaaag tatgtcagcc aatgaagtta gacctaatct gcaagcatag tttcctcttt
     7561 atcctcagtc tatatggaaa caaatttaag cccaatccct attggaaagt cccaacttag
     7621 tttttaggga atgaaatcgt aactgggaaa ctcataaatt acaagtgcga ttcctttatt
     7681 agtatccgat ttcaaataat ttattcctgt ttgaccctaa aacagtaagt catttgcatt
     7741 attcaatttt aaagtaccaa gtaaattatc ataactgacc gctactaagc ctatactgaa
     7801 cagagccaag gggagtgacg gtccgtttaa ttcccttctc acacactagg gtaaccaacg
     7861 agagtcttgt tcgtaacatt atattgcaac agcaagcctg tgctgagtga aatcgaagga
     7921 actaactggc tatgcaatgg attcaatagt ttatttgcat ttgtgaaagg agaaatccat
     7981 ctttttattg accttggtat aatagacctc tcactttctt ttgattttac aacatatatt
     8041 ttcggaaccc tgctacctgc ttaaccttta tttaatacta ctaccagcca ttccgattta
     8101 agtatagtta aatatattgt aaaatgtata acataaaaag cataactaat atttagtgca
     8161 gtaatatgaa attgaaagta agataaagta agattgagta aaaatgactt tttgccctgc
     8221 cttactttgt tttcggttac attttccgat gccagaaaca gaatacgagt gaagccggtc
     8281 aaggagaaac agtgacgaac aaagatcaat caagtcatta cagataagat agtaaaatcg
     8341 aatacgccaa cctgaaattt gaaggcaaaa ggtatgtata acgaccttat aaagacatat
     8401 caaaaaataa caaggatatt cagagactta atcatattca caataattgc aatttttacc
     8461 tttgtgttag caattgtatt tatatttgat gcatttgaga agctcgtcga attgactaga
     8521 aaatatgaaa tagtcgaaat cgatgaactt attactctac tcgcaggttt gtcatttgct
     8581 tttagcattt tctctttcct aaggtggaaa gaacttagac atgaaacaat taagcgtaag
     8641 caggttgagg aggaattaaa taatagaaat aaggacttag aaattctaaa tactatcact
     8701 caagcagtac atcagtcctt tgatctgaag gaggtctata ggattgcgtt agatcaagta
     8761 attgaactag ataatgttga ccttgcctgc atatatttag tttacgaggc caaaaatgta
     8821 gcagtaatgc aggaccaaag gaatttccct gaggagtttc tacaaagggc tggggtaatt
     8881 ccatacccta agggtgcaac ctggaaggtg ataagcacag gaaaaatatc gaatgttaaa
     8941 aatgcgcaga cagatccgga tgtaggacct gctggacgcg accttggatt ccgtagtatg
     9001 ctcgggattc ctataaacct tgaaggaaaa acgataggag taatatggct gcttagttat
     9061 aaagaatatc tctttactac gcgtgaagag gggttacttg tatctattgg gaatcatatt
     9121 gcaacagcta tagcaaaggc aaagctatat ggagagttat ccaaaaagag tcgttacgag
     9181 acaatcatta gcgctgtgac aaggagtgtc catcagtcaa tagaactaca agaggttttg
     9241 gaaaacgccg ttgatgcaat gagtaagaat ataagtgggg tagaacacgt tgcaattcac
     9301 ctagtcgaag gagaagaagc ggttatcaaa gcccatagag gccaccctga ttggtttgtc
     9361 gagcgtgtaa gaagaattcc ctatccaaaa ggctttacct ggaagacaat aatagagggg
     9421 aaacccaggt attgtccaga tgtagaccag gatacagtta tcggtcctgc tggaagggag
     9481 gtcggaacaa agagctattt gtccatgccc attcgcgacg aggttaagac agtagggtgt
     9541 ataaatgtac attcttttcg gaagaattcc tttgacgaag aagaaataaa gttattagag
     9601 attgtagcac agcaaatcga aacggcgatc aaaaatgcac agcatgcgga ggcgcttcga
     9661 gagagcgaag aacgatttcg caatatggca gatacagccc ccgttatgat ctggatgtct
     9721 gctcccgaca agctttgcaa ttacttcaat aaagtctggc tagaatttac tggacggaca
     9781 gtggaacaag agatgggcaa cggttggact gaaggtgtgc atcctgacga tttacagcgt
     9841 tgtttagaca cctattttac tgcgtttgat gatcgccggg atttcaaaat ggattaccgc
     9901 cttaggcgtt ttgatggtga gtatcgctgg attattgatg caggcacccc gagatttatg
     9961 catgatggcg ggtttgccgg ttacattggc tcagcgattg acgtcgctga gcgcaagcat
    10021 atagaagatg aacttaagac gctaaacgct atcatggaag cggttcatag gtcatcagac
    10081 cttaaagagg tttttaatat cgctctagac aaggttagag aactaacgga tatagatatt
    10141 gtagggatat acttggtgga tgagggtaca aatgaagcta tgctagaggc tcatagagga
    10201 taccccgata tatatataga aagagcagga aggattccat atcctaaggg tgtaacatgg
    10261 aaggttatca actcaggtga gaactatgtt gttcaagatg tatcaactga cccatacgtt
    10321 ggtcccgtcg gtaaggaatc tcgatttcaa agctttatgt cggtacctat aaagatggtg
    10381 gataaggtaa taggtgctat acactttcat agctacaaaa agaataaatt tggtaaacgg
    10441 gaaatagagc ttttttcttc cattggcact cagatcgcca tagccgtagc aaaggcaaaa
    10501 cagagggaag acttacagtt agcaaatgaa gatttatccg tcctaaatgc aatagctacc
    10561 tctattcata agtctttaaa tcttcaagag ttatacaata ttgctctaga tactgtttta
    10621 gaaataacag cctttgatat cattatgatt tatcttgtgg atgaaaatac aaatgaggct
    10681 gtacttcaag catatagagg actcacagag gactatatca agagggcagg aaggatacca
    10741 taccctaagg gagtaacatg gaaggttatt aactcaggtg aactgaccct tattgatgac
    10801 attcaaaaag acccaaacct tggtcctgct ggtagggtat tgggtcacca cactatgttg
    10861 atagtgccta taaagcagga ggagaaagca ataggaataa taggttttgc cagtctcaag
    10921 gtgttagagc ttagttctag agaaataagt gttcttaatg ctataggcag tcaaattgga
    10981 acagcaatag tgcaggcaag actctacgaa aaatcccaga atcagtcaga ggagctaaga
    11041 gctttatatg aggacttaga tagaagaaat agggacttag agatactgaa tactataact
    11101 caagcagtgc atcaatcctt taatttagat gaggtttaca atgttgcctt agatgcggtt
    11161 ataagtttag aaaatgtgga tatggctatt atttatttgg tagatgacga tagaaaagaa
    11221 gctattgtcc aagcccatag gaatttccct gaggattacc tgcaaaaggc taggagaata
    11281 ccatacccta agggtattac ttggaaggtt attaacaccg gtgagatgat caatattgaa
    11341 gatgcccaaa aggatcctga gataggtcct gcgggcaggg aactaggaca tcatagcctt
    11401 ttaggagtac ctatacttct agagggaaag gctgcagggg ttatctggtt cctaagctat
    11461 aaggaacgca agtttgctga gagagaggcg aacttacttt ctactcttgg taatcagatc
    11521 tcaatagcta ttgcaaaagc aaggctatat agggatttga aaaagacaaa tgagaaactt
    11581 aaggagctag acgtgatgaa agatgagttt gtatcaatgt cttcccatga gcttagaact
    11641 cctatgagtg ccatagcagg ttacgtctcg atgctttttg aaggtgactt tggcgaactg
    11701 ccttctgaag caaaagatgc tttaactgat gtaaacacag ccacacagcg tttaattagt
    11761 ttagttaatg atatactcga cgtatccagg attgagcagg gaaggttaga actgaaaatc
    11821 ttagaatttg atttaggaga aatttgccaa gaggtattag acaatctgac ccctatatct
    11881 caagaaaaag ggataaaact tgcctaccag aagtcggcag aggttgtgag accgttggtt
    11941 tcagccgata tgaataaggt tatgcaagtt ctcaacaacc ttgttgggaa tgcgttaaaa
    12001 ttcacccaaa agggtgaagt aattataact caccgctttg atggtaacct gattatcacc
    12061 gacgtgattg ataccggagt tggaatttct tctcaagacc agcaatttct ctttcaaaag
    12121 tttcagcagt tagataaaac cctttccggc aaccgcttgg gaggaactgg gttgggactc
    12181 tacttttccc gtgagcttgt gagagcgatg gatggggact tatggctcga aagaagtgaa
    12241 ataggagagg gaagcacctt ttccttttcc ctgtctaggg caaaagctat ggggagctag
    12301 tctcgaagga aagagtgccg tagaagttag tttataaatg gagagaggag gctaagatgc
    12361 ctaagctttt attagttgag gacgacgaga ttcttattag gatgtatcga acaaagttag
    12421 agcgcgaaaa ctttgaggtg gatacagccc aagacgggga agagtgtttg acaaagatta
    12481 gggaatttaa gcctgacatt gtgcttcttg atattgttat gcctaaacta aacggattag
    12541 atgttctaaa aagggttaaa gcccaacccg aattaaaggg aatacctgtg gttatactaa
    12601 ctaacgcacc agttcatatt gctgccaagg aatgtttaca attaggagct ttgggatata
    12661 ttatcaaaag cgataacact ccaagtgagg tcgtcaaaaa agtaagagaa tttttaaata
    12721 tggggtgaaa agtggcggat tccaataaat ctaaagtctt aatgatagag gatgaccccg
    12781 tgctccagag gatgtacata agtaaactca cagagggtgg ttatgaggta gaccttgcca
    12841 gagatggcgc tgaagggctg cataaagcgt taaacaccga ccctgaccta gtgcttttgg
    12901 atcttatgct tcctaaggta gacgggctcc aaatcctggc tgaaatgaga aaaaacccta
    12961 agacgcgaaa aacccctgtt atagttctca gtaacctagc acaggctgac gatgcaacga
    13021 aggccctaca actaggagca gacgattatg ttgtaaagtt gaacacccct ccaagcagtg
    13081 tattgaagaa agttcagatg ctcttatcac aaaggaaggc aagggatgct atctacacca
    13141 gtcggtacca tattaagatt gatactactc aggcagatgc gccgcagctt gtacgagact
    13201 ttggccttat atttttctgt gctcattgta ggacacaaca aattcttgaa ctcatgcctg
    13261 attataactc cactggttcg gggcatcgtt tcgtagcgca gtttgtttgc cctatttgca
    13321 aatcggttta ttaaacacct ctacctaatc tctgatcaaa atccatccct aaatgccctc
    13381 ctttctcgtg ctcttgaagg ataacttcag tttcagctat aatatcaaag ctaactaata
    13441 cattaaagga gaaaccagca tggcaagtgc aggaatagta gtgttaagcg attccacctt
    13501 tgaaggcgag gttttaaaga gtgaatcacc ggttttagtt gatttctggg ccccctggtg
    13561 cggcccctgc aggatcctag ccccagtagt tgaggagatt gcaaactcct atactggacg
    13621 tattaaagta ggaaagctca acgttgatga caaccaagaa accactatgc aatatagtat
    13681 aagaagtatc cctaccctta tactttttaa aaatggaaag gccctagatc agattatagg
    13741 agccgtcccc aagagtgaaa tagagaagat ggttaaaaaa gctctttagc tattaaaagc
    13801 taatctgaag cttatataat agttaagaat acaaaggtac gtgttattag ttaagtacgt
    13861 gaataaataa aaaacctttc ctctataagt ctatcaatgt tccacgatta aagcatgaaa
    13921 agtaattttt gagtgagatt tccagatttt ggtagtttga atgctattca agaatataat
    13981 ttaaaaaaga gttattaggt aaacccgacc taacgcatcc tcagtgcgaa tgagctcgac
    14041 ccatcttgga gaccctagag cggtcaatct gaaaccgcca gtaaaacccc ctcagagaca
    14101 acatatttaa cactttgtaa acaaccaatc atccatagct agacggtttt gcgactcagc
    14161 cagccttcga gatgagaaac gtcacccctt acaatctcgc ggacacaaca ctcttttgaa
    14221 tctaaactta atttaaggtg atgaaatgct gcccatgggt ctaccgccgt accacaggtg
    14281 aaaatatcta cggctgcata ctcatattct ggccacgtgt gaattgccag atgagactcg
    14341 gcaataacca caacaccact tacaccttgg ggtgtgaagt aacgaaacga actttccagc
    14401 actgtggctc cgcaatgtga ggctgcctcg agcatacatt gtttaactct ctccaagtca
    14461 tttagaactt tagggtcgca tccaaggtat tccgccaaga gatgcctccc tagggccctc
    14521 aatctctcct ccttcgtcct tcaaggtatt ttgcatagaa agtcagaata tacattccta
    14581 gctaaaaaag tcaaacaaat tttgacgaat caaacaattt tcaattcata attaactcct
    14641 tagtaaaccg tcaagttgag tgagctatta gatattgtca agtactcagg caccgtactc
    14701 ttcataatag tcttccattg ctgcctcgac tacttgtaga gcatgctcgc agttgtaaat
    14761 tttctcgatg gtcatctgag attgcgttcc aagctcaagc ttgtaggtaa taaagtagtg
    14821 ccgtaagcgt tcgactagaa ttgccggcag gtgcgaaatg tcttcgatt
//
LOCUS       DATDRL010000017         3274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1548966, whole genome shotgun sequence.
ACCESSION   DATDRL010000017 DATDRL010000000
VERSION     DATDRL010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548966"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..930
                     /locus_tag="VI935_00745"
     CDS             196..930
                     /locus_tag="VI935_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HLE24157.1"
                     /translation="MKILVLSDIHANWYALEAILAKESYNALIFLGDVVDFGPNPRKC
                     VRFLMDSSRNRFWGVRGDHDHALAYGINCGSSLNLRELSMKTREWGEGLVASDEVGFL
                     RRLPFENHATLDGLKFHFMHGSKRDRLFRSITTDIGELEIENEFGETDTDFILIGHSH
                     KPFIIHLGRVTVLNPGSVGQPRDFNPRASYAVIEDGKAMIKRVRYDIEKTVRDLEKSS
                     LPKDVIRELVSILVIGGLVKDEIYYC"
     gene            complement(1217..2419)
                     /locus_tag="VI935_00750"
     CDS             complement(1217..2419)
                     /locus_tag="VI935_00750"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HLE24158.1"
                     /translation="MSEAVTVEFNKTFNRMMNEVVALGSCCGCSMCVVTCPYGVIKYD
                     HQSMFPYVAKPKGDFDYCPISEEVGCDVCANACERLGPEQMKPIKPEEIDVHVFGRAR
                     REDEYYGVTRFICAARSTNPSVVANAEDGGAVTEILKCAFEDGIIDGAAVAAPSDKMW
                     LEPAPRVVKSVDELMQTAKSWYTYCATPLALKEAVDKHKLSKLALVGVSCEVSGYGTT
                     VGADSSFIAAERGEKNVERQNKHLKQYVDAVQFTIGLLCTETFVFDGFIHEYLQEKVG
                     INPQDVVKVNVKGKVVTDIKDGTRIETPLLEARVYQRHQCNYCGDFSAEHSDISAGGV
                     GSDGWTILIVRSERGERIFKRTIEKGYLEVRPASEFERSMKVLKRLTETQRKRADEQY
                     ISIGSLRR"
     gene            complement(2419..>3274)
                     /gene="cofH"
                     /locus_tag="VI935_00755"
     CDS             complement(2419..>3274)
                     /gene="cofH"
                     /locus_tag="VI935_00755"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009831997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HLE24159.1"
                     /translation="EIAEKTKEARELGATEVCMQGGMHPDIDGRFYVELIKTVKKAVP
                     DMHTHAFSPFEIYYGAKTLDISEEDFIEMLKDVGHNTFPGTAAEILDEEVRTIIAPRK
                     IPTEVWIRVVKKAHKAGMRTTSTIMYGHVDKPYHWAKHIGVLRDIQKETGGFTEFVPL
                     RFIPWKTRLYRKSKGKVRPGPTDLDQLKMYGVSRLMLRGWINNIQVSWVKQGPEFAQF
                     SLTAGANDFGGTLMEEHISRAAGAEHGQYFPPEEFRRLTRGIGRIPAQRSTTYEIIKT
                     FSREEVEE"
BASE COUNT          902 a          776 c          595 g         1001 t
ORIGIN      
        1 agacaaatgg ctttttagtt gcgtgtttct ttggattata gcaacagcca taattattta
       61 tttccaacat aacccaagca tcttgagata atcgctgacg aagtttaaga cccgaaaata
      121 gaaatttttt acctaacaca atcttaactt cttctttata ctccattaat aaattaaaga
      181 tagaatacca atactatgaa aatcctagta ctttcagaca tacacgctaa ctggtacgca
      241 ctcgaagcca tactggctaa ggagagctac aatgccctca ttttccttgg tgatgtggtt
      301 gattttggac caaatccacg aaagtgcgtc aggtttctta tggattcatc cagaaaccgc
      361 ttctggggtg ttagaggaga tcatgaccac gctcttgctt atggcattaa ctgcgggtca
      421 tcgttaaatc ttagagaact atctatgaaa acacgagaat ggggagaggg tttagtcgca
      481 agcgacgagg ttggttttct aaggaggctc cctttcgaaa accacgcaac acttgacggg
      541 ttaaagttcc atttcatgca tggctcaaaa agagataggc tttttagaag cataaccact
      601 gatattggag aattagaaat tgagaatgaa tttggagaaa ccgatacaga ctttatactt
      661 ataggccatt ctcataaacc cttcataata cacttaggga gagtaactgt tttaaatccg
      721 ggttcagtgg gacaaccaag ggattttaat ccaagggctt cttacgcagt catcgaagac
      781 gggaaagcaa tgataaagag ggtaagatac gatattgaaa agactgttag agacttagag
      841 aaaagctcgt taccaaaaga tgtcatcagg gagctcgttt ctatacttgt aattggtggt
      901 ttggtaaaag atgaaattta ttattgctaa aagcaatata gtttggcaag cggtttttga
      961 taataaattt ctttaattcc ctattgatgg cattgtaaaa aaatagtgtc tgtcactatt
     1021 ttcagggaag aatattaagt ccctctttgc taaagaggga tttagggaaa tttgaatctc
     1081 cctaagctca tttaaaaagg aaaggattaa atcccctctg gctccccttt ttttgaaagg
     1141 ggagaaaata atcaaaatca attgtgtaaa ggtggtttga gttgtggctt gattaaatct
     1201 cgcctttact ggacttttat ctccgcaaac ttcctattga aatatattgc tcgtctgccc
     1261 tttttctttg tgtttcagtt agtcttttta ataccttcat agaacgctcg aattcagatg
     1321 caggtctaac ctcaagatag cccttttcaa tcgttcgttt aaaaatcctc tctcctcttt
     1381 cagaccttac gattagtatt gtccatccgt cagaacctac gccacctgct gaaatatccg
     1441 aatgctcagc gctaaagtca ccgcaatagt tgcactggtg tctctgatag accctagcct
     1501 caagaagagg tgtttcaatc cttgttccat cctttatgtc tgtaactacc tttcctttca
     1561 catttacctt gaccacatct tgaggattta ttccgacctt ttcttgaaga tactcgtgga
     1621 taaatccatc aaaaacaaag gtctctgtgc agagaagtcc tattgtgaac tgaactgcat
     1681 ctacatactg cttgaggtgt ttattctgtc tctctacgtt tttttcacct ctttcagcag
     1741 cgatgaagct actgtccgct cctaccgtcg tcccgtatcc cgatacctcg catgaaaccc
     1801 caactaacgc aagcttactg agcttatgct tatctacagc ctctttaaga gccaaaggag
     1861 ttgcacaata tgtgtaccag gattttgctg tttgcatgag ttcatctaca ctcttaacta
     1921 cccttggcgc aggctcaagc cacatcttat ctgaaggagc agccactgct gcaccgtcaa
     1981 ttatcccatc ttcaaaagca cacttaagaa tctcggttac cgctccgccg tcctcagcgt
     2041 ttgcaaccac acttggattt gtgctccttg ccgcacaaat gaaccttgta acgccatagt
     2101 attcatcttc acgccttgct cttccaaaca cgtgaacatc aatctcttca ggctttatag
     2161 gcttcatttg ctcaggtccc aatctctcac acgcatttgc gcagacatcg cagccaacct
     2221 cttcgcttat agggcagtaa tcaaaatccc ccttaggttt tgccacatac ggaaacattg
     2281 attgatggtc gtacttaata accccataag gacatgtgac aacgcacata gaacagccac
     2341 aacagctccc aagcgcaacg acctcattca tcatcctgtt aaatgtctta ttaaactcga
     2401 ctgtaacagc ttcgctcatt attcttccac ttcctcccta ctaaatgtct ttattatttc
     2461 ataagtggtt gacctctgag caggaatgcg tccaatcccc cttgtaagac gacgaaactc
     2521 ctcaggtggg aaatattgtc catgctctgc tccggctgct ctcgatatat gctcctccat
     2581 caatgttcca ccaaaatcat ttgctcctgc agtaagtgaa aactgcgcaa actcagggcc
     2641 ctgctttacc catgagacct ggatattatt aatccatccc cgaagcatga gtcttgaaac
     2701 accatacatc tttaattgat caaggtctgt cggaccaggc ctaactttac ccttagattt
     2761 tctataaagc cttgtcttcc atggaataaa ccttagtgga acaaactctg taaacccacc
     2821 tgtctctttc tggatatccc taagaacgcc tatgtgtttc gcccaatggt agggcttgtc
     2881 tacatgtcca tacattatgg ttgatgttgt tctcatccca gctttgtgag ccttcttcac
     2941 tactcgtatc cacacctctg tagggatctt tctaggcgca attatcgttc ttacctcctc
     3001 atctagaatc tcagcggcag tgccagggaa ggtgttatgt ccgacgtcct tgagcatctc
     3061 tataaaatcc tcttcactta tgtcaagggt ctttgctcca taataaatct caaatggaga
     3121 aaacgcatgc gtgtgcatat ctggcacggc tttctttacc gtcttaataa gctcaacgta
     3181 aaatcttcca tctatatcag gatgcattcc gccctgcata caaacctcag ttgcacccaa
     3241 ttcgcgtgcc tcttttgttt tttctgcaat ctct
//
LOCUS       DATDRL010000018        13313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3921349, whole genome shotgun sequence.
ACCESSION   DATDRL010000018 DATDRL010000000
VERSION     DATDRL010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13313
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3921349"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..1099)
                     /locus_tag="VI935_00760"
     CDS             complement(377..1099)
                     /locus_tag="VI935_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24160.1"
                     /translation="MKKIKVLGAPVIVVAVMLSTLLAGPGIVCAHNPGFTDSFMFEEC
                     DGFSSIGDNPFFSLEPGFELILEGEEDGVDIGLTITVLNQTQTITGIKTRVVEERETH
                     DGELVEVSRNYFAICNRDNSVVYFGEDVDFYENGVIVGHEGSWRAGVNGARAGVIMPG
                     IQLLGARYIQEVAPGVALDRAETNSISEVVETPAGTFKNCLKTKETTPLEPGVVGFKF
                     YAPDIGLVQDGMLKLVEVFEAD"
     gene            complement(1331..2716)
                     /locus_tag="VI935_00765"
     CDS             complement(1331..2716)
                     /locus_tag="VI935_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLE24161.1"
                     /translation="MFLKNPRLLLKSSSFRLITWYTLLFILCSLVINVYAYTIISSFI
                     YEQSRKEIEEDIDDLAEIYQKDGLEALREDVYEEEDEPFLIRLIGNQGNTLISKIPND
                     WSGFNIEQLEKSGYLKNKEWKYLKGGDDENEFEITSLSLSNGSTLQLGQNIVEREDLL
                     RRIRKIYMIAIIPIILFAYLGGIFVADRALNPIRQLINTLKSIVSSGKINVRVPVYQT
                     DKLHDELISLFNTMLEKIEALVSGMRNVLDNVAHDLRTPITRLRGTAEVALQSEQDPD
                     ILREALSDCIEESERILIMLNTLMDVSEAETGAMKLDPEEMNVAPLIEDIVGLYGYVA
                     EEKGVSVYTGFPEELYLRADRNWIRQVLANLLDNAIKYTPSGGRIDIEAYRRDGEVTI
                     TVKDTGVGIPPEELEKIWDRLYRGDKSRSQRGLGLGLSLVKAIVRAHRGYVEVSSNPG
                     AGSVFTVYLPV"
     gene            complement(2697..3371)
                     /locus_tag="VI935_00770"
     CDS             complement(2697..3371)
                     /locus_tag="VI935_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLE24162.1"
                     /translation="MRVLVIEDDKKIASFVVKGFKQAGFAVDHASDGDDGIHLALHES
                     YDAAVIDIMLPKLDGLSLIEELRRNKINTPVIILSAKRSVDDRIKGLQTGSDDYLTKP
                     FSFSELLARVQALIRRASGAAETTLLTFGNLSMDILKREVTRADKRINLQPREFALLE
                     YLMRNAGRMVSKTIIMEHVYDYNFDPQTNVVDVLVCRLRDKVDRDFEKKMIHTIRGVG
                     YVLKES"
     gene            complement(3675..4454)
                     /locus_tag="VI935_00775"
     CDS             complement(3675..4454)
                     /locus_tag="VI935_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24163.1"
                     /translation="MKSIKVLAALVIGVAVGVLSLGVAAPEAWAAPLSAAKIIFETNA
                     TAGDTGIQVFLDGEPWKNVMIINPDGLQIFEVQGQGGLEGLEGFGLTELFSESNEPPF
                     EEVPLAEVLAMFPAGKYRFEGTTVEGASLKSMATLSHVIPCGPEVVSPAEGAMVNQPV
                     VIDWEKVTKEFDPDSDPNFDPDNDCKESSVLEIVGYQVIVEQQKPQTPPLVFSVFLPA
                     DTTEVTVPPEFIEVDTKWKFEVLAIEAGGNQTITESFFETN"
     gene            complement(5140..5544)
                     /locus_tag="VI935_00780"
     CDS             complement(5140..5544)
                     /locus_tag="VI935_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HLE24164.1"
                     /translation="MIEKNKKENIGEEIKRAKEAMRVTYLLFENSFVNDAISKLYYFL
                     LYNVRALLLTKGLEPKSHEGALRLFGLHFVKKGIFNPRDSHIFSKLMKFREEADYNPS
                     YTFTNEDFIEFKKEAEELADKIRKYLRKKRYI"
     gene            complement(5541..5885)
                     /locus_tag="VI935_00785"
     CDS             complement(5541..5885)
                     /locus_tag="VI935_00785"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLE24165.1"
                     /translation="MAYGKNNLLPKEKAALIELKKSLNGFLRERLIKFVLFGSRARED
                     YDNKSDIDLAIIVRGLTRNLKNQVLEKVADIEFKHLVPISTLVLSEVEFENLKRLERR
                     IALDIEREGKPL"
     gene            6160..8631
                     /gene="pheT"
                     /locus_tag="VI935_00790"
     CDS             6160..8631
                     /gene="pheT"
                     /locus_tag="VI935_00790"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HLE24166.1"
                     /translation="MRFTLSWLKEYVDFDASPEELAERLTMVGLEVESIEYMGMGLED
                     VFVAEILDIRPHPNASKLVICDVTDGSKLYNIVCGAKNMKAGDKVALAKAGTRLPPSV
                     RFPEGLLIQSTKIRGELSEGMLCAENELGIGEDGEGILILPEYAMIGERLVDALGLND
                     VVIGIGVTPNRPDCLSVIGIAREVAAILATKVKYPDYGVLEEGEDIENLAAVEVLDVD
                     GCPRYSCRLITDVKIAPSPDWLKTRLEASGIRSINNVVDVTNFILLELGQPLHAFDHN
                     LLVGRKIIVRPAKNGELFETIDRIKKTLTNEDLVICDARKPIALAGVMGGSNTEISEK
                     TRNILLESAYFNPARVRRSSKRTNLKSESSYRFERGVDPNGVVKALDRASELIRKVAK
                     GKVAKGKIDIYPSPIRPKEVEISTRRINSILGTDIKTEKIKEIAERLEIQALTAKDGE
                     ISLCVPTFRVDLMRETDLIEEVARHYGYNSIPLTLPVVPMKTENSKREKNLENKVKEI
                     LPSFGFLEVVNYSFDDSELLSLYSEAEQLKILNPLTKESSVMRTNLIVGILRNVSLNL
                     NHQVQDIRIFEIGKVYFSKAGGLPKEITKISGAATGKRQLELWDKEEFDFFDMKGILE
                     RVFEAFSISQYVRFVDSSIRHVYPEQGRRAHDSPQDDASEIRFLHPGKSARVLAEGEE
                     LGFFGELHPEFQEKLGLSKRVYIFEIDLEKLISLEKTVEKKFVSLPKFPYVRRDIALV
                     VDDNISLGEILNEIRRMKSPLIEDLRVFDVFKGGNVSQGKKSIAVSMILRASDKTLTD
                     EEVNEVQKMAISGLQKALGAELRKT"
     gene            8833..9120
                     /locus_tag="VI935_00795"
     CDS             8833..9120
                     /locus_tag="VI935_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HLE24167.1"
                     /translation="MTKKELADVIHTRIGLSKREAAEIVEFFFEIVKEKLSNGEGFKI
                     PKFGSFRVQSRKARKGRNPATGETIEIIKRKAVVFKPSRFLRDAINKKSPV"
     gene            9120..9785
                     /locus_tag="VI935_00800"
     CDS             9120..9785
                     /locus_tag="VI935_00800"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HLE24168.1"
                     /translation="MKRITRSLRRNFLAGLLVTVPFGFTVFILFKLGKWIISLVSAAP
                     AKVLIKPLSELHPLLFQVLTFATGFFGTLLIIFIVGAITRNYIGRKLLDIGERLISKI
                     PFARTIYIATKQMIETIFLGTGFKGISRVVIFEYPRKGIYSIGLVTGIPRHTESGKRL
                     LGIFLPTAFNPTGGYYFMIPEDEVKELPISVEDAFRIIISGGLASNQLETAKPSKEDD
                     KTL"
     gene            9787..11115
                     /gene="tilS"
                     /locus_tag="VI935_00805"
     CDS             9787..11115
                     /gene="tilS"
                     /locus_tag="VI935_00805"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HLE24169.1"
                     /translation="MLASDETVIVGVSGGADSVGLLYILTELKEYRLKLIVSHLNHGI
                     RPKDGKRDAEFVKGIAASLNLPFELKEVNTPMYKKSSNLSLEEAGRILRYEFFKEVSD
                     KYQAQKIATAHTLNDQAETVLMRILRGSGRVGLSGIPPVSEGNIVRPLIETKRSEIEE
                     FLRMKGVEWVEDSSNWARDFLRNRIRHELISELEKYNPRIKETLARTARLLRTEEDFI
                     KKEAGKKLGSVFTILDQDELVGTTSRYKTIPEAIRLVVLRLAVEKLKGNLRKISSDHI
                     FSVDELLFSKKPSGEISLPDGIVVIKGYDLFLVTRKSELMREISYRIPSTGKWRFPGL
                     EFEVEITKVKRPGEDKSIGFFDADSVEFPIEVRSFRPGDRFIPLGMRTFKKVKRFFID
                     EKVPRFLRNRIPIFLSKGKIMWISGMRVDERFKLLGKTAVKIKLIKPKIY"
     gene            complement(11306..12004)
                     /locus_tag="VI935_00810"
     CDS             complement(11306..12004)
                     /locus_tag="VI935_00810"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLE24170.1"
                     /translation="MSLELKIKDDETLDGPYGLKILQKKDGYRYSQDSLQLMDFALVK
                     KNDEVIDLGTGCGVMALILARKGFGKRIVGLEIQKELAELARRSVELNGFQKKIEIVE
                     GDIRKVKSLFIPSSFDYVITNPPYIEAKSGLISPGSERALARHEILCDMNDILEAIKY
                     LLKPSKRGSCVYPASRLDDLIIKAKKKGLEPKKVQFVHPGPEEKAELVMVEFIKEGRP
                     GLEVLPPLYSKLAF"
     gene            12456..13073
                     /locus_tag="VI935_00815"
     CDS             12456..13073
                     /locus_tag="VI935_00815"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HLE24171.1"
                     /translation="MRLTTDKIIFLIACLISIFWQLEGGISQELDKKNNWKPTEGLEL
                     IGTKAPSFEGLNWLNTEPINIEDLKGKVVLIRFWLVGCPLCEHTAPALVELYYKYKDD
                     GFIVVGIHHPKSEDTKNPDLVRRAMNVFSFDFPVAQDNDWKVINSYWLGGKKRSFTSS
                     SILIDKNGIIRFVHDGGEFYRSGSNPDADRAYQAIEEKIQELLGE"
BASE COUNT         3949 a         2816 c         2767 g         3781 t
ORIGIN      
        1 tcggatcgtt ccgtcctcac tctcaagcac gctctcactt tccggaacca cagggaagga
       61 atcgttgtcg atgtctagtg agaatgggcc gccggtttcc gggtcgcgaa ctttaatgtc
      121 cagattgggc tctgggcatg caccaccccg ccactgttgt tgcacccaac cccccagagg
      181 acaaggaaaa gtgaaaaaca ttaggcaaat agatgaatac atcgtttcat ggtttagcca
      241 tccttgtaag tattttttac tatcacaaac ttagatatat atagaagttt tcaattccat
      301 gtatggctct ataggcggga ctttccagtc ccgcattgcg tggcagggtt agaaaactca
      361 gcctatccta aacctcctag tcagcttcga acacctcgac cagtttcaac atcccatcct
      421 gtactagccc aatgtccggc gcatagaatt tgaatcctac gaccccgggc tctaacgggg
      481 tcgtctcttt tgttttgaga cagttcttaa acgtgcccgc cggagtctcg acgacctcac
      541 tgatactgtt ggtttctgcc ctatccaggg caaccccagg agcgacctcc tgaatatatc
      601 tcgcgcctaa taactgaatc ccaggcatga ttaccccggc tcttgcgcca tttactcctg
      661 cacgccaaga tccttcatga cccacaataa ccccattttc atagaaatca acatcctccc
      721 caaaatagac gacactgtta tcccggttac atatggcaaa gtagttcctt gaaacctcaa
      781 ctaactcgcc atcatgcgtt tctctttctt caaccaccct cgtttttatg ccagtgattg
      841 tttgtgtctg gttgagaacg gttattgtca aaccaatatc gacaccgtcc tcttctcctt
      901 ccaaaattag ctcaaaacca ggttccaagc tgaaaaaggg attgtctccg atggacgaaa
      961 acccatcgca ttcctcaaac ataaaggaat cggtaaaccc cggattatgg gcgcagacga
     1021 ttcccggccc tgccaagaga gtggacaaca taaccgcaac tactataaca ggcgctccca
     1081 atacttttat ttttttcata ctgcacctcc tgaattggat tttatgtaca tgatatcagg
     1141 agacaattac gggaaccttt cctgaacatt acttcttgga aaggtttaca aaatgtgaag
     1201 atggatgaac cagattgaat aattaataat tagtcataga gagcacagag aacacaaaga
     1261 aaagaattaa aaataaattt tctgtttttt tctctctgtg aactctgtgg ctagacaaca
     1321 tcagttaaac ttaaacggga agataaaccg tgaagacaga gccggcgccc gggttgctgg
     1381 agacctcgac gtatcctcta tgcgccctga cgatggcttt gacaaggctt aagcccagcc
     1441 caagtcctct ttgagaccgg ctcttatcgc cccggtacag acgatcccag attttttcaa
     1501 gctcttcagg ggggattccg actccggtat ctttaacggt gatagtgacc tctccgtctc
     1561 tccgataggc ttcgatgtct attcttccgc cactcggggt gtacttgatt gcattatcta
     1621 gcagatttgc cagtacctga cgtatccagt tgcggtctgc tcttaagtaa agctcctctg
     1681 gaaaacccgt atatactgaa acgccctttt cctcagcgac ataaccgtag agcccgacaa
     1741 tgtcctctat caggggggcg acgttcatct cttccggatc gagtttcata gctccggtct
     1801 ctgcttctga tacatccata agggtattta acattatgag aatccgttca gactcctcta
     1861 tgcaatcgct caaggcctcc cggagaatat ctgggtcctg ctcagactgc aatgccactt
     1921 cagcagtgcc tctcaatctc gtaatcggcg ttcgcaagtc atgtgcgacg ttatccaaaa
     1981 cgtttctcat tccacttaca agggcttcga ttttttctaa catcgtatta aaaagcgaga
     2041 tcaattcatc gtgcaattta tcagtttgat agacgggaac tctcacattt atcttgccgc
     2101 ttgaaactat cgatttaagc gtatttataa gttgtcgaat cggattcaga gcgcggtcgg
     2161 caacgaatat tccgcctaaa taagcaaaca gtattatagg gatgatggcg atcatatata
     2221 ttttgcgaat tctccttaaa aggtcttccc gctcgactat gttctgaccg agttgcaaag
     2281 tcgagccgtt ggacaagctt agagacgtta tttcgaattc attctcgtca tcgccgcctt
     2341 ttagatattt ccactccttg tttttgagat agccgctttt ttcaagctgc tcgatgttga
     2401 aacctgacca atcattcgga atttttgaaa tgagcgtatt accttgattt ccaataaggc
     2461 gaatgagaaa gggttcgtcc tcctcttcat atacgtcctc tcgtaaagct tccaatccgt
     2521 ctttttgata tatctctgca aggtcgtcta tgtcctcctc gatttccttt ctgctttgtt
     2581 catagataaa agaagaaatg atggtatatg cgtaaacatt tattacaagc gagcatagaa
     2641 taaagagaag ggtgtaccaa gttatgagtc taaagctgct tgatttcagt aagagcctag
     2701 gattctttaa gaacataacc aactcctcga atagtgtgaa tcatcttttt ctcaaaatcc
     2761 ctgtcaacct tatctcgcag tctgcaaact aatacatcca ccacattcgt ctgcgggtca
     2821 aagttgtaat catagacatg ctccataata atggttttgg aaaccatcct tcctgcatta
     2881 cgcataaggt attcgagtaa ggcaaattca cggggttgaa ggttaatcct tttatcagct
     2941 ctagtaacct ctcgtttaag aatgtccata gagaggtttc caaatgtcag gagagtggtc
     3001 tcagctgcgc cgctggctct gcggatgagt gcctgaacac gtgcaagaag ctccgagaaa
     3061 gaaaatggct tagtcaggta gtcgtcgctc cctgtttgaa gccctttgat cctatcatct
     3121 accgatcgct tggcgcttag aataatcacg ggtgtattta tcttgttccg ccggagctct
     3181 tcgataaggc tcagaccatc caatttaggc agcattatat ctatgactgc cgcgtcgtag
     3241 gactcgtgta gagccagatg aatgccatcg tcgccgtcag acgcatggtc tacggcgaat
     3301 cccgcctgtt taaatccctt aacaacgaaa gaggcaatct ttttatcgtc ttctataacc
     3361 agtaccctca aagctcatct cctcgttcga caatactcat aagaatttaa attaatccga
     3421 gttcgatgta aagatctttg aaagcggact gtcgttacgc cgaccctgcg cttaccgaag
     3481 ccccagtttc tcaatctcta agaaccctct tcccttgttg cagctaacgc tgccccggcg
     3541 ataggcgatt gacgctaggg ttcatttgca acggatacca ttggtaccat tcgggtcgcc
     3601 tctcaacttt agagattcat tttctcgtta ctcgttatct caaatgttga ggcctgcccc
     3661 tttgtttatt ctttctaatt cgtctcgaag aagctctccg taatcgtctg gtttccgcct
     3721 gcctctatcg ccaacacctc gaacttccac ttcgtgtcta cctcgatgaa ttccggcgga
     3781 accgtcacct ctgtggtatc ggccgggagg aagacgctaa acacgagtgg gggggtttgg
     3841 ggtttctgtt gctcgacgat gacctggtag ccgacaattt caagtactga tgattcttta
     3901 cagtcattat cggggtcaaa attggggtct gaatcggggt caaactcctt tgtaactttc
     3961 tcccaatcaa tgacaactgg ttggttcacc attgccccct cggcaggtga gacaacttcg
     4021 ggcccacatg gaataacgtg cgaaagcgtc gccatgctct tcagactggc tccctcaacc
     4081 gtcgtgccct caaacctata tttcccagcc ggaaacatgg ccaggacctc tgcgagcggg
     4141 acctcttcaa acggcggctc attgctctcg gagaacagct ccgtaaggcc gaacccctcc
     4201 aacccctcca agcccccttg accctggacc tcaaagatct gcagcccgtc cgggttgata
     4261 atcatgacat tcttccaggg ttctccatct aagaagacct gaatgcccgt atcgccagcc
     4321 gtggcgtttg tctcgaagat aattttcgca gccgacaagg gtgcggccca agcctccggg
     4381 gctgcgaccc ccaaactgag gacgcctacc gcaactccaa taacaagcgc tgctaatact
     4441 tttatacttt tcatactgca cctcctgaat tggttttatg ttcataatat caggagacta
     4501 ttacgggaac ctttcctgaa cattacatat tggaaaggtt tacaaaagtt gaagtggggt
     4561 ggatcagaag ttaaagggga agataaacgg tgaagacagt gtggggtcta gtctcaacgg
     4621 gctgttgccc taaccctctc acaaatagtt gtaaaattga tataatggga aaaacttaaa
     4681 ggaggataaa agccgccagc catgatgagt taccaagacc caccacagag gaagttgttt
     4741 tatataggta ttaatctgga taagaggata agaaagaatc ctccacgagc ttttgacctt
     4801 ttttttgaac ttatctatcc ctgactgtta aatatttcca tacctaacct tcttgatcgt
     4861 aagatttctt attcaaaaac aagaagacca ccgcaacgct gaaaagctat aggtaactca
     4921 catattataa tattccctaa tgtagagacg ccattaatga cgtctctgta aattaaaaaa
     4981 gtggtggaag gttttgggat ataagaattt aaatttttcc ttggttcata caatgagcat
     5041 tgcaaacacg ggtaatatcg atcccactgc tgtacacatt aatcaaggat gataatttaa
     5101 aacataaggt ctgaaaacaa tataacaagc actcgacaat caaatatacc ttttttttcg
     5161 taagtacttc cttattttat cagccagttc ctccgcttct ttcttaaatt caataaaatc
     5221 ctcgtttgtg aatgtatatg agggattata atcagcttcc tctctaaact tcataagctt
     5281 tgaaaatata tgagagtccc taggattaaa gatgcctttt tttacaaaat gtagaccaaa
     5341 aagcctaagc gccccttcgt ggctttttgg ttcaagacct tttgtaagaa ggagtgccct
     5401 tacattatag agcaggaagt agtacaactt tgaaatagcg tcattaacaa aactattttc
     5461 aaaaagaaga taagtaacac gcatcgcttc ctttgccctt ttaatctcct cgccaatgtt
     5521 ctcttttttg ttcttctcta tcataatggc ttgccttccc tttcaatatc aagggctatt
     5581 cttctttcca accttttaag attttcaaat tcaacttccg agagcacgag ggtagagatc
     5641 ggaacaaggt gtttgaactc tatatcagca accttttcaa gtacctgatt tttaagattt
     5701 cttgtaagac ctctgacaat gattgctaag tctatatcgg atttgttatc ataatcctcc
     5761 cttgcccgtg atccaaagag aacaaatttt ataaggcgtt ctcgtaaaaa accattaagg
     5821 ctttttttta gttcaattaa tgctgccttc tctttaggca acaggttatt tttcccatat
     5881 gccactaaaa atactcccat atgtgattta tcatcattat acattaattc catttttctc
     5941 taacacagtt aaaaaggcta ttataaaaaa tcattgcaag tattgaaaac cggaaaatag
     6001 agaagattcg tattatcttt tgtcacattt taaaagtttg cagttcacta ttaccttaat
     6061 gcagatttgc ttttagcagg caagcaaata taacatactc aaattacaca aaatattgac
     6121 atttcatctt ttaatatgtt accttgattc acaaataaga tgagatttac tctcagttgg
     6181 ctcaaagaat atgttgattt tgacgcctcg cctgaagaat tagcagaaag gctcaccatg
     6241 gtagggcttg aggtagaatc cattgaatat atgggaatgg gattagaaga tgtattcgta
     6301 gccgagattc tagatataag accacaccca aacgcatcaa agcttgttat ctgcgatgtt
     6361 acagatggca gcaaattata caacatagtt tgcggggcaa agaatatgaa agccggggat
     6421 aaagtagccc ttgcaaaagc aggaacaagg ctccccccga gtgtaaggtt tcctgaaggc
     6481 ttattaatcc aatctactaa gattcgaggt gagttatcag aaggtatgct ctgcgctgag
     6541 aatgaactag ggatcggcga agatggtgag ggcattttga ttcttccaga gtatgcgatg
     6601 attggtgaaa gattagttga tgctcttggg cttaatgacg ttgttatagg aatcggcgtc
     6661 acaccgaatc gtcccgattg cctgagcgta attggaattg caagagaggt cgcagctatt
     6721 ttagcaacaa aggtaaaata ccctgactac ggggtattag aagaaggaga agatatagag
     6781 aacctagccg cagtcgaagt gcttgatgta gatgggtgtc ctagatactc ctgccggcta
     6841 attacagatg taaaaatagc accttcccca gattggctaa agactcgact cgaagcctca
     6901 ggcatcagat caattaataa cgtagtagac gttacgaact ttatacttct cgagttaggt
     6961 caacctcttc acgcatttga ccataaccta cttgttggaa gaaaaatcat agttagacct
     7021 gcaaaaaatg gagagctctt tgaaactatt gatagaataa agaaaacact aaccaacgag
     7081 gatctggtta tatgtgacgc acgaaaaccc atcgcgcttg ccggggtaat gggcggatca
     7141 aatactgaga tttcagaaaa aacaagaaat attctccttg aaagtgcata ttttaatcca
     7201 gcgagggttc gaagatcctc aaagagaact aacctaaaat ctgagtcttc ttaccggttt
     7261 gaacgggggg tagacccaaa cggggttgta aaagcgcttg atagggcatc tgagcttatt
     7321 agaaaggttg ctaaaggaaa ggtagctaaa ggtaaaattg atatataccc aagtcctatt
     7381 agaccaaaag aggtagagat ctctacgaga cggataaatt ctatattggg cacagatatt
     7441 aagactgaaa aaataaaaga aatagccgag aggttagaga ttcaggcctt aacagcaaaa
     7501 gacggtgaga tttcgttatg tgttcccacc tttagggtag atcttatgcg tgagactgat
     7561 ttaatagaag aggtggcaag gcattatggg tataacagta ttcccttaac ccttccagtt
     7621 gttcctatga aaacggagaa ttcaaaaaga gaaaagaatc ttgagaacaa ggtaaaagaa
     7681 atactccctt cgtttgggtt tctagaagtg gtcaactaca gctttgatga ttctgagctt
     7741 cttagtttgt atagtgaggc tgagcaactt aagatattga atcctctcac taaagaaagc
     7801 tctgtaatga gaaccaatct gatcgtaggt attttgagaa atgtcagtct taatctaaat
     7861 catcaggttc aagacataag gatatttgaa attggcaagg tgtatttctc aaaagcaggc
     7921 gggcttccta aagaaataac aaaaatctct ggggcggcaa cagggaaaag gcaactggag
     7981 ctgtgggaca aggaggaatt tgatttcttt gatatgaaag gaatcctgga aagggtattt
     8041 gaggctttct ccatttccca atatgttcga tttgtggatt cctccattcg acatgtctat
     8101 cctgagcaag gtcgaagggc tcatgattca ccacaggatg acgcatcaga aatcagattc
     8161 ctccacccag gaaaatctgc aagagttctt gcagaaggcg aggaacttgg gttcttcggg
     8221 gagcttcacc ctgagtttca ggaaaaactc ggactttcaa agagggttta tatctttgaa
     8281 attgacttag aaaaattaat ctcacttgag aaaacagtgg aaaagaaatt cgtctcgctt
     8341 ccaaaattcc cttacgtaag aagggatatt gccctcgtag ttgatgataa tatatccctc
     8401 ggagaaattt taaatgagat tagaaggatg aaatcccctt taattgaaga cttaagggtg
     8461 tttgatgttt ttaaaggcgg aaatgtatct caagggaaaa agagtatagc cgtttctatg
     8521 atcctgaggg cctcagataa aacactcact gacgaagagg taaacgaggt tcaaaaaatg
     8581 gcaataagcg ggcttcaaaa ggcccttggg gcagaattac gaaaaaccta aaatattaac
     8641 cactaaggcg ctaagacaca aagaaggaat atgagtttta acttgatagt caggagtcag
     8701 aagagagaat atcgtgaggt gtaagtcgtt agacgtaagg ggtcaggaga tcttcttacc
     8761 ccccttacgc ctcacgccct attcgtgtct tggtgtattc gtggctaaat aaattacaaa
     8821 gagggaaagg ttatgacgaa aaaagagctt gccgatgtaa tccatacaag gatcgggctt
     8881 tcaaagagag aggctgccga gattgtggag tttttctttg agatagtaaa agagaagctc
     8941 agcaatggag aaggatttaa gattcccaaa tttggtagct ttagggttca aagtagaaag
     9001 gccaggaagg gtaggaaccc cgcaacaggc gagacgattg aaatcattaa gaggaaagca
     9061 gtcgtcttta aacctagcag atttcttcga gatgcgatta ataaaaaatc acctgtttga
     9121 tgaaacgtat tacccgatct cttagacgga atttcctagc tggacttctt gttactgtgc
     9181 catttggatt tacggtattc atactcttta aactcggcaa atggattatc agccttgtaa
     9241 gcgctgcccc tgctaaggtt cttattaaac ctctttcaga gcttcaccct ctcttatttc
     9301 aagttcttac ctttgcaaca ggattttttg gaacattact aatcattttc attgtcggag
     9361 caattacaag gaactacata ggtcgaaagt tgcttgatat aggggaaagg ttgatttcaa
     9421 agatcccatt tgcaagaacg atttatatag ccactaaaca gatgattgag acaatctttc
     9481 ttggcacggg ttttaaaggc ataagtcgag tagttatctt tgaataccca cgaaaaggga
     9541 tatattcgat aggacttgtc acagggatcc cacggcatac agaatcagga aaaagactct
     9601 tgggaatatt tcttcccact gcttttaacc cgactggcgg atattatttc atgatacccg
     9661 aagatgaagt aaaagagctt ccaataagcg ttgaagacgc atttaggata ataatctctg
     9721 gaggactagc gtcaaatcaa cttgaaactg ctaagcctag taaggaagac gataaaacgc
     9781 tttaatatgc tcgcttctga tgagacggtt attgtgggtg tttctggagg agcggattct
     9841 gttggattac tttacatact gactgagctt aaagaatata gattgaaact catcgtttcc
     9901 cacctaaatc atggaataag acccaaagat ggaaaaagag atgcagaatt tgtaaaggga
     9961 atagcagcta gccttaacct tccatttgaa ctcaaggaag tcaatacacc catgtataaa
    10021 aagagttcga atctttcgct tgaagaggcg ggaaggattc ttagatacga gttttttaag
    10081 gaggtctcag acaaatacca ggctcaaaag atcgcaactg ctcatacgct taacgaccag
    10141 gctgaaacgg ttcttatgcg gattttgaga gggagcggaa gagtcgggct ttctgggatt
    10201 cctcctgtat cagaaggtaa tatagttaga ccgcttatcg aaacaaaaag gtcggagatt
    10261 gaggagtttt taagaatgaa gggcgttgaa tgggttgaag actcgtcgaa ttgggcaagg
    10321 gattttctta gaaacagaat aagacatgag ttaatttcag agcttgaaaa atacaatcca
    10381 agaattaaag agacgcttgc taggactgca aggttattga ggactgaaga ggattttatt
    10441 aaaaaggagg cgggcaaaaa gcttggaagt gtattcacta tattagatca agacgaactc
    10501 gttggaacca cctctcgata taaaactatt ccggaggcaa taaggcttgt cgtcttaaga
    10561 ttagcagttg aaaaattaaa agggaatcta aggaaaatct catccgatca catattctct
    10621 gttgatgaac ttcttttttc taaaaaacca tcaggtgaaa tctcgctacc tgatggaatc
    10681 gtcgtgataa aagggtatga cctcttttta gttacccgaa aatccgagct aatgcgtgaa
    10741 atctcttata gaattccttc tacaggaaaa tggcgttttc cagggcttga atttgaggtt
    10801 gaaattacta aagtgaagcg cccgggtgaa gataaatcca ttgggttctt tgatgccgat
    10861 tcggttgagt ttccgattga agtaagaagc tttcgtccag gagataggtt cattccgctt
    10921 ggaatgagaa catttaaaaa ggtaaagaga ttttttattg acgaaaaggt ccctagattc
    10981 cttagaaatc gtattccgat attccttagt aaaggcaaga tcatgtggat tagtggtatg
    11041 agggtagatg aaagatttaa gttgctagga aaaacggcgg ttaaaatcaa attaatcaag
    11101 ccgaagatat attgattccg gatttgtgat ttcggattgc agatttaaaa aatcttttat
    11161 tccgaaatcc acattccgat tcgaacaatt tgaagactaa ggtcttatac caattcaaga
    11221 gatgaattct aaatgcaggg cggttttatg gtatttaatt taaacagtaa tataacccat
    11281 ttagaatcta tttttcaaaa tggtattaga aagctagctt gctatagagg ggtggaagga
    11341 cctcaagccc aggtctccct tccttaataa attcaaccat aacaagttcc gctttttctt
    11401 cagggccagg atggacaaat tgtacctttt taggctcgag cccttttttc tttgctttga
    11461 taatcagatc gtctaacctc gaagcggggt agacacacga accgcgcttt gacggtttaa
    11521 gcaaatattt tatcgcttct aaaatatcgt tcatatcgca aagaatctca tgccttgcta
    11581 acgccctttc ggacccaggg cttatgagac ccgatttagc ctctatataa ggggggttcg
    11641 ttataacata gtcaaaggaa ctaggaatga aaagggattt aacctttctt atatcaccct
    11701 cgactatttc tatttttttt tgaaaaccgt taagttctac gcttcttctt gcaagttcag
    11761 caagttcttt ttgaatctca agcccaacga ttctcttccc aaaacccttt ctagccaata
    11821 taagagccat gacaccacac ccggttccaa ggtcaataac ctcgtcgttc ttctttacaa
    11881 gtgcgaaatc cataagctga agcgaatcct gtgagtacct atatccatcc ttcttctgaa
    11941 gtattttaag cccgtacgga ccgtctaggg tttcatcatc ctttattttc aattctaagc
    12001 tcattaattt tcccacgtat taaaactaat acacaaataa atgatacacg atgcacgata
    12061 cattaatatt attcgtcttg catcgtgtat tctgcatctt gcatcatgtc cattttattc
    12121 gtggtcatag ttctatcgag cctatccttg tctaagaact caaacgaagc ccttaatacg
    12181 aagacacgct aatttttagt catacttaac aaaatatcaa tgacaataga ctccttacta
    12241 attgctgggt attttattta cagcgtgcca taaaacagga cctctgattg cgttatcgca
    12301 aagagctagc catattatct gtcattgcga ggagagaagc gacgaagcaa tctgatacta
    12361 agtgccattc cggagtgagg aatctcaaag ggattgctta acggaggtta cactgaacga
    12421 agtgaatgtg ttcgcaatga cactgatgga aagtcatgcg tttaactaca gataaaataa
    12481 ttttcctaat cgcttgtcta atcagcatct tttggcaact cgaaggtggt atatcacaag
    12541 agctcgataa aaagaacaac tggaagccta cagaggggtt ggagcttatt ggaaccaaag
    12601 cgccctcttt tgagggactg aattggctta atacagaacc tataaacatt gaagatttaa
    12661 aaggtaaagt agttctaatc cgcttctggt tagtgggttg cccattatgt gaacacacag
    12721 ctcctgccct cgttgagctt tactacaaat ataaagatga tggttttatt gtagttggaa
    12781 ttcatcatcc taaatcagaa gatacaaaaa atccagattt agtccgacgt gctatgaatg
    12841 tatttagctt tgatttccct gtagcacagg ataatgattg gaaggttatc aattcctatt
    12901 ggctaggtgg taaaaaacgt tcgtttacct cttctagcat tctcatagat aaaaacggca
    12961 tcatccgatt cgtacatgat ggaggcgagt tttataggag tggaagtaac cccgatgccg
    13021 atcgtgccta ccaagccatt gaagaaaaaa tccaggaact attgggagaa taagcaaaaa
    13081 attaatgccc gttctgggaa cgggcgctac atttggtgtg gcaatatcaa acattgcttg
    13141 gtacgttccc tgaacgtacc aagcaaaaat taatgccttt tgagagaata taaactacca
    13201 cgacccgcct ccgcctccgc caaatcctcc accgctcgat ccaccgcctc caaatccgct
    13261 acctccagag tatccaccag atttactggg agtggatgca aaggtactgc tca
//
LOCUS       DATDRL010000019         4247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_217540, whole genome shotgun sequence.
ACCESSION   DATDRL010000019 DATDRL010000000
VERSION     DATDRL010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217540"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VI935_00820"
     CDS             complement(<1..331)
                     /locus_tag="VI935_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009671941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLE24172.1"
                     /translation="MVERVIAPKLQEEDVLFESNLRPLNLRDFLGQTKAKEKVEIFIE
                     AARRRQESLDHVLLYGPPGLGKTTLAHIVANELGVKIHATSGPVIERIADLASILTHL
                     NDGDVFFI"
     gene            complement(451..1038)
                     /gene="ruvA"
                     /locus_tag="VI935_00825"
     CDS             complement(451..1038)
                     /gene="ruvA"
                     /locus_tag="VI935_00825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HLE24173.1"
                     /translation="MISLLRGKIAEKSLGKIVIDIQGVGYGVTVPLSTYYRLPERGNE
                     TELKIYTHMKEDGIELFGFLTADERKIFTSLLGVAGVGPRVATNILSSISPGELVSAI
                     SSGDLVKKKIPGIGPKLALRLITELKDKVSGIQPAGDGGKEKEILEDIISALINLGYK
                     RNEIDERISQLEEVTSKENDIEKALKESLKIMRRV"
     gene            complement(1016..1567)
                     /gene="ruvC"
                     /locus_tag="VI935_00830"
     CDS             complement(1016..1567)
                     /gene="ruvC"
                     /locus_tag="VI935_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HLE24174.1"
                     /translation="MRVLGVDPGSRVCGYGVLESRNGELIHIESGCITAPSSFSLPLR
                     LKNIFEGLREVIERSSPDAMSIEDIFFAKNSKSALKLGEARGVALLAATISGIPVHEY
                     APTEVKLALTGQGRAKKLEVQKMISLILGISEWSKEDASDAVAIAICHINSSQINERL
                     GREVIRHRRRRRRWKKYDISSPR"
     gene            complement(1573..2316)
                     /locus_tag="VI935_00835"
     CDS             complement(1573..2316)
                     /locus_tag="VI935_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLE24175.1"
                     /translation="MSGHSKWSNIKHRKAAVDAKRGKIFTKLIRELTVAARLGGDPNT
                     NTRLRIAIAAAKNQNMPKDTIDRAVKRGTGEIGGEELHEIFYEGYGPGGSAVLVEVLT
                     DNKNRAVSEIRRIFTKYGGNLGESGCVSWMFEKKGRIAFEKGKVDEDKLFEIVLEAGA
                     DDVRTEENEVVVITSPDNFESVKAAIEQAGVKYASAEVTMVPKNNVRIEGKEAEQMLR
                     LMENLEDSDDVQNVYSNFDIAEELMQALG"
     gene            complement(2420..3385)
                     /locus_tag="VI935_00840"
     CDS             complement(2420..3385)
                     /locus_tag="VI935_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24176.1"
                     /translation="MNNNNRLKVVVVSLFFISCAFFLISGLLAQMLPSQALVQSSEQQ
                     IREEKRKSFSETITYVQVEFSSLIGEIFVNPRYTGRITTIRTFLKDGSPRDNPIVDVR
                     ETKDRWEKVVVSESESDAVELIATGRKGSLKVSLSKEKAPSEVDKLQLLNQIIISFGQ
                     LSTTLITPGIEISNPAGFEIGILDFKEDKFLNGDIIAIKNNQVAVGFHNLSSIVVNKD
                     GMIRLSLKTPDGSFVGADLKAWGYNMLVGEIEVGKPLPIKAEVFGLPEDAKLKFTFEP
                     LPEQNITPNTKTLTVKDINKGTPIAKIVTSIPGVQPLRVIVEKVD"
     gene            complement(3473..4045)
                     /locus_tag="VI935_00845"
     CDS             complement(3473..4045)
                     /locus_tag="VI935_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24177.1"
                     /translation="MIKLKPIALTIIVLVLFSSCAAWKNGSTESKVGPELEVWTSPLG
                     FVPSPPSLVSVRPYCCPSTAIEVTTSMPIRENDYQWINLGLTLPSKRNTKEVVITEFE
                     LCYTIESASSGTTYISQTRLSKITTSDSSLVIRDDSTRLASTTPTCHKVSAFDARIDG
                     TINLELKLVFGDTSDKIRIGGIKFTLDSRE"
BASE COUNT         1165 a          962 c          725 g         1395 t
ORIGIN      
        1 ctataaagaa aacgtctcca tcgtttagat gggtaagaat cgaggctaaa tctgcaatcc
       61 tttcaatgac gggaccggac gtcgcgtgga tttttacacc gagctcattt gcaacaatat
      121 gtgctagagt agtttttcca agacctgggg gaccataaag aagaacgtgg tctagagact
      181 cttgcctcct tcttgcggct tcaataaaaa tctctacctt ttccttagcc tttgtttgac
      241 caagaaagtc tcttagattt agtggcctga gattagattc aaaaagcaca tcctcttctt
      301 ggagttttgg agctatgact ctttctacca tttaattata taaataacat actcataatt
      361 ttttagccac aggagacacg gagaaaagat ttaaattttt cgtctttttt cttttctctg
      421 tatcctcttt gccctctgtg gctgatttcc ttacaccctt ctcataatct ttagggattc
      481 ttttaaggct ttctctatat cgttttcttt tgaagttacc tcctcaagct gcgatatgcg
      541 ctcatcaatt tcgttccttt tatagccgag attaattagc gcagagatta tgtcctccaa
      601 tatttctttc tcttttccac catcccctgc aggttgaatc cctgaaacct tatccttaag
      661 ctcggtaatg agccttaatg cgagcttggg gccgattcca ggaatctttt tcttaacgag
      721 gtcccctgaa gaaatagctg acacaagctc tccaggagat atgcttgaga ggatatttgt
      781 tgctacccta ggtcccacgc ctgcaacacc aagaagcgat gtaaaaattt ttctctcatc
      841 cgccgtaaga aaaccgaaaa gctcaatccc gtcttctttc atatgggtat agatcttaag
      901 ctctgtctcg tttcctcgtt caggaagacg gtaatatgta gacaggggaa ccgtaacccc
      961 gtatcccaca ccttgtatat ctatcacgat ttttcctaga gatttttcag cgattttacc
     1021 tcggagaaga gatatcatac ttcttccagc gacgcctcct tcttctgtgt ctaattacct
     1081 ctctacccag tcgttcattt atttgagatg aattgatatg acagattgca attgctactg
     1141 catctgaagc gtcttcttta ctccattcag aaattccgag tattagagaa atcatctttt
     1201 gaacctctaa tttctttgcc ctaccctgtc ctgtaagagc aagttttacc tcggtaggag
     1261 cgtactcatg tactggaatt ccagatatgg tcgcagcaag aagcgcaact cctcttgcct
     1321 ctccaagctt tagagcgctt ttagaatttt ttgcaaagaa gatatcttca atagacatcg
     1381 catcgggcga cgagcgttct attacttctc taagaccttc aaatatgttt tttaacctta
     1441 atggaaggga aaaactggag ggtgctgtta tacagccact ttctatatga attagctccc
     1501 catttcggct ttctaaaact ccataaccac atacccttga gccgggatca actcctagaa
     1561 ccctcattca acttatccaa gagcctgcat aagttcttct gcaatatcaa aatttgaata
     1621 gacattctga acatcgtcgc tatcttcaag gttttccata aggcgaagca tctgctcggc
     1681 ttcttttcct tcaatcctta cattgttctt tggaaccata gttacttcag ccgaggcata
     1741 cttaacacca gcttgttcta tagcagcctt aacagactca aaattatcag gtgaagtgat
     1801 tacgacaact tcattttctt ccgttcttac atcatccgct ccggcttcga gcacaatttc
     1861 aaagagctta tcttcatcta cttttccttt ttcaaaagcg attctgccct ttttctcaaa
     1921 catccacgaa acgcacccac tttccccaag gtttccgccg tattttgtaa agattctcct
     1981 aatctcagat acagcccgat ttttattgtc agtaagcacc tcgacgagaa ccgcgctccc
     2041 cccaggacca tatccctcat aaaaaatctc gtgaagttct tctccgccta tttcaccggt
     2101 gccacgcttt acagcacgat ctattgtatc cttgggcata ttttggttct tagctgcagc
     2161 aattgctatg cgaagtcgtg tattagtatt cgggtcgcct ccgagtcttg cagctacagt
     2221 aagttccctt ataagctttg taaatatttt cccacgcttg gcatccactg ccgcctttct
     2281 gtgttttata tttgaccatt ttgaatgacc agacataata aactcacccc tcttttataa
     2341 atctatctaa tatagaatat ctgtttgata aagcattgta aagtaggggc acgttgcaac
     2401 gtgcccctac aatcactctt taatctacct tctcaactat aactctaagc ggttgtacac
     2461 cggggataga ggttacgatt ttcgcaatag gtgtgccttt gtttatatct tttacagtaa
     2521 gtgtcttagt atttggtgtg atattttgct ccggaagcgg ctcaaaggtg aatttgagtt
     2581 tggcatcctc tggaaggcca aatacctccg ctttaatagg gagaggttta ccaacttcaa
     2641 tttcacctac aagcatgttg tagccccatg cctttaaatc agcacctacg aagcttccat
     2701 caggtgtttt gagactcagt ctaatcatcc catctttatt cacaacaatt gaagacaaat
     2761 tatggaatcc caccgcaacc tgattattct ttatcgcaat tatatcgcca tttaggaatt
     2821 tatcttcttt aaaatcaaga atacctattt caaatccggc tggattggag atttctatac
     2881 ctggagtaat tagagtggtg ctaagttgtc cgaaactaat aatgatttga tttagcaact
     2941 gaagtttgtc cacctcagaa ggcgctttct ccttagaaag cgagactttt aaactacctt
     3001 tccttcctgt agcaataagc tcaacagcat ccgattcaga ttcagaaacc actaccttct
     3061 cccatctatc tttagtttct ctaacatcaa caatagggtt atcccttgga gaaccatctt
     3121 taagaaatgt tctaatagtt gtaattcttc ctgtatatct tgggtttaca aaaatttcac
     3181 ctattaaaga actaaactca acctgaacat acgtaatagt ttcagagaaa ctcttccttt
     3241 tttcctctct aatctgctgt tccgacgact gaactagtgc ttgcgagggt aacatttgtg
     3301 ctaataaccc agatatcaaa aagaaggcac aagaaatgaa aaagagtgat accacaacta
     3361 cctttagtct gttattgtta ttcatattct cctcttcaac atgaagtttt ttttcatttt
     3421 ggatatttgt gccatttcgt ggattatcag gtatttctga tcagcatata aatcactccc
     3481 tggaatccaa ggtaaatttt ataccaccga tacggatttt atctgaggtg tctccaaaaa
     3541 ctaattttaa ttcaagatta attgtcccgt caatccttgc atcaaatgcg cttaccttat
     3601 gacatgtggg ggttgtgctt gcaagtcttg tagaatcatc acgaattaca agcgagctat
     3661 ctgaagtggt tatctttgaa agtcgtgttt gagagatgta ggttgtaccc gaagaagccg
     3721 attctatcgt atagcagagc tcgaactctg taataactac ctcttttgta tttctctttg
     3781 acggaagtgt tagaccaagg tttatccact gataatcatt ttctctaata ggcattgatg
     3841 tagtaacttc aatagcggta ctgggacagc aataaggtct tactgaaacc agtgatggcg
     3901 ggcttgggac aaaaccaagt gggctagtcc atacttcaag ctcggggcct accttcgatt
     3961 cagtagaacc attcttccaa gccgcacatg aagaaaaaag aactaatact attattgtga
     4021 gtgcaatagg ttttagcttg atcacattta ttcccctttt acttaatagc gatttgatat
     4081 tattttcata actatgataa attacactgt tagctaaaat ttaaaagtga actctttaag
     4141 aattaaaagt gaactatatt taactcacta tttttaaccc ttttgacatc aataagttta
     4201 tcattgcacc agaatcttac ctcagatatt ccattactca taggata
//
LOCUS       DATDRL010000020         8906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_486759, whole genome shotgun sequence.
ACCESSION   DATDRL010000020 DATDRL010000000
VERSION     DATDRL010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486759"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..576)
                     /locus_tag="VI935_00850"
     CDS             complement(106..576)
                     /locus_tag="VI935_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HLE24178.1"
                     /translation="MKNSALSFLMALSLIFSVFGFSSLLSSCATSQRTYDAGAVLGGP
                     AGSLVDRENSWRGGVLGAAIGGVLAGTVTEISARAAVEAAEEGRPVIYQSADGYQRVE
                     ETPVSYNAEAKCHKVRERIWQEGQLVKDEVKEVCQSEKKGYLLIQQQQNKLSSQ"
     gene            complement(637..1014)
                     /locus_tag="VI935_00855"
     CDS             complement(637..1014)
                     /locus_tag="VI935_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HLE24179.1"
                     /translation="MKRLAQIRGDFKSALRRLDEALKDAKTDLEVDGAIQRFEFTFEL
                     IWKLLRLFLEDQGIICRTPKACLKEAYRIGVIDDEVTWLNILDDRNMSVHIYDKETSR
                     KIFKRVKTKYIKEFKKVFEKVGK"
     gene            complement(992..1333)
                     /locus_tag="VI935_00860"
     CDS             complement(992..1333)
                     /locus_tag="VI935_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLE24180.1"
                     /translation="MAPKPRDDNENETKKPGLSMSLLENIISVIKRYIEPSRIIIFGS
                     RARGDYDKTSDIDIAIDCKGDVGFINEILGEDAPTLLKINLVNLRKVNKELRKEIIRE
                     GIIVYEEASTD"
     gene            1753..2127
                     /locus_tag="VI935_00865"
     CDS             1753..2127
                     /locus_tag="VI935_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24181.1"
                     /translation="MKIRAAIIVVKDWKERAFLRAELIEEGVRTFAVERMGEAEEWLA
                     DPEMLPIIIIYDTNNQDSPSRDLMRLSELITHIPVLIITSFLEKGASNLEELGFKHII
                     QRPIRIGDVVKYVKEILKGTKR"
     gene            2633..4213
                     /locus_tag="VI935_00870"
     CDS             2633..4213
                     /locus_tag="VI935_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24182.1"
                     /translation="MHSNNEKSGRPEDNCTGLAVFTFLFVWATIFELPVTAPLPQNFS
                     AISLNNLTKLVFVFLAIWAILKPSSLFRSIPFFAYTLLAIVITLPAVPNHVFVQIIIL
                     TTILTCFIFSIIKERSNFTTTKFYELFAPILRLELIIIYFWAAFHKLNTGFLNYKISC
                     ATVQIFNIKDMIPFFPTPDWFIHINPYLTLLIEGLIPILLIIPKTRIYGFVLGICFHF
                     ILGFQYPGFTVFAYSLYSLFIPSSSYDKIKTKVAELMDRIWEALPGISNYKHWKKTMF
                     GNITINVVLVLLIFFILRVFKAGGQRTLPPVSEVGLYLIFCTFLGITFIYFVVRRLKE
                     LRIDERLVFIPQKKWLLIFPAIVFLNGLLPYIGLKNIQVFAMFSNLQTEGGKTNHLLI
                     PSSFQLFNNLEDLVSIKRSNNKMLNQFSQGVFRKIKGVTTVLIPKSYVEYMKDKNENF
                     KIILKYKIPFVMLQNIVTRGTQLGVKNIELEYERGGETFNAKNAELDPLLNNASAFQI
                     IFLPQRAVPDDERGLCMW"
     gene            4549..7113
                     /locus_tag="VI935_00875"
     CDS             4549..7113
                     /locus_tag="VI935_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5808 family serine peptidase"
                     /protein_id="HLE24183.1"
                     /translation="MLPSVMQHIKRHYANKSAIDPKAMLISGLGRLERNLDEVLVDLP
                     DGENGTSFRVQVINDEKTFDMNGVNDLDSLTQKIEEVFKFVTPRLVSRDLTPDEIEYA
                     VIDEMLNKLDRHSGIITPQIYREFMIETEGSFGGLGIVIGIRDGQLTVIAPIEGTPAY
                     NAGIKPNDKIVQIEYESTVNMSLIEAVGKLRGPKGTNVNIYIMREGFPDSKKFSIVRA
                     TIKIESVEAYSLGDGIGYIRIRDFQRNTLDSIKASLRSLKKNGSLNGLILDLRGNPGG
                     LLDQAERISNLFLKSGIIVTTSSGDSKKPYKATPEDYEYGGRVVVLVDSGSASASEIV
                     AGALKNNERAIVVGDTTFGKGSVQQIFDLNDGSALKLTIAQYLTPGDISIQDIGITPD
                     IQISPVIVSKDAYNTSSENHLINELLNGEKKGKLENKEKPIYTIFYLETQKDTQNKGE
                     ETPDEALSREEKLKNIENDFSVAFAKDILLQSKSHSRKEILNQVQTGIKEISENEERK
                     IVGKWQSEGVDWSIGKRGSGVPALSIKVAPSPLKAKAGEKLEVKAQLENTGQIPLYRL
                     KAVTESDNPIFNGKEFIFGKLGLGEKMESAVTFEIPKGILQREDTITLKFVDFYKSDL
                     PGFTFNAQIEELPRPAFAFNYEIVDDGRMDSSGNGNGIPETGESIGLLVKVKNIGSGS
                     SEKGIFTLKNLSGDSIFLQKGRFEYKDLHPGEVKDAPFIFEVKKPVQQIEMEFGILDD
                     VFREELRDKFRLTHKEENRNQVFKEPPSISFSNLSHSTKSQEIALNGLVKDQNGLELV
                     SVFVGDDKVILLPSSTKEVPLSMNVKLNKGVNLITVLARDKDGLTSRESVVVRFGE"
     gene            7110..8030
                     /locus_tag="VI935_00880"
     CDS             7110..8030
                     /locus_tag="VI935_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLE24184.1"
                     /translation="MTKFILGRVLSGAFVVLIVATLTFFLLRLLPGGPFDTEKKVPPE
                     IRKNIEEKYRLNEPLWKQYYIYIRDLAHGDFGPSYKYIDRTVRDIIKETLPVSVKLGT
                     AALLISLTIGTGIGIFSAARPRGLFDILSLSFATALVSVPSFVVGAALIYIFSVELDW
                     FPAALWGRPENYVLPAFTLAAGPTAYLARLTRASMLEVSQATFVRTAWAKGLGEFKVI
                     LKHIFRNALIPVVTVLGPITAFLVTGSFVVEHIFSIPGMGRFFVFAVSNRDYPLVMGI
                     TIVYTVILVIANLLVDICYVILDPRIKFGK"
     gene            8094..8828
                     /gene="truA"
                     /locus_tag="VI935_00885"
     CDS             8094..8828
                     /gene="truA"
                     /locus_tag="VI935_00885"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HLE24185.1"
                     /translation="MKNIKLVLEYDGTNYLGWQKQPHGITIQEVIENTLNKITSETIT
                     VIGSGRTDSGVHALAQVANFKTEIRMTSLQFQKALNSVLPKDIVVKEVSEVDINFHAQ
                     LDVKSKTYLYKILNRPYPSALERNRVWFLPESLDVPKMAEAARLLGGEHDFRGFALSN
                     TTKTTVREVMNVNLKKKPDGLVEFEIESTGFLRGMVRLIVGTLVQVGKGKIAPEDFRE
                     ILESGEKTNLVFSAPPSGLYLKEVRY"
BASE COUNT         2736 a         1790 c         1825 g         2555 t
ORIGIN      
        1 tcattgcgag ccgaaggcga agcaatctcg tttttaaatt gcttcgtccc cttcgctttg
       61 ctctgcgctt cggctcactt gctcctcgca atgacagccc tattatcatt gcgatgacag
      121 cttattttgc tgttgctgta ttagtaagta gccttttttt tctgattgac aaacctcttt
      181 tacttcatcc ttcacgagct gtccttcttg ccaaatcctt tctctcacct tgtggcattt
      241 tgcctcagca ttgtagctaa cgggtgtttc ttcaaccctt tgatacccgt cggcgctctg
      301 ataaataaca ggacgcccct cctcagcggc ctcaactgcg gctcttgcag aaatttcagt
      361 gaccgttcca gcgagaacac cgccaatcgc agctccaaga acaccccctc gccaactgtt
      421 ttccctatca accaatgagc ccgcaggccc accaagaact gccccagcat cgtaagtgcg
      481 ctgactagta gcgcaagatg aaagaaggct tgaaaatccg aagaccgaaa atataaggct
      541 taatgccatt aaaaaagaga gcgcgctatt tttcatgatt gtcctccata ataacatgta
      601 taaactacga actgagagaa aagttgatag aggaacttat ttccctacct tttcaaatac
      661 ctttttaaat tccttaatat atttggtttt aaccctctta aatatctttc ttgaagtttc
      721 tttgtcatat atgtgaacac tcatatttct atcatcaagt atattaagcc aagtaacttc
      781 atcgtctata accccaattc tatatgcttc ttttaaacat gccttgggag tccggcaaat
      841 aatcccttga tcttcaagaa atagtctcag tagtttccaa attagttcaa atgtaaactc
      901 aaacctttga atagcgccat ctacttctaa atctgtcttg gcatctttaa gcgcctcatc
      961 caatctcctt aatgcgcttt taaaatcacc tctaatctgt gctagcctct tcataaacaa
     1021 taatcccttc tctaataatc tcctttctca attctttgtt tacctttctc aaattgacta
     1081 gattgatttt taaaagggtt ggtgcatctt cacctaatat ctcattaata aagcctacat
     1141 cccccttaca gtcaatagct atatctatat ctgaggtttt atcgtagtcc ccccttgccc
     1201 tagaaccaaa gattataatc ctacttggtt caatgtaccg tttaattaca ctgatgatat
     1261 tttctaataa gctcatagat agacccggct tttttgtttc attctcgtta tcatccctgg
     1321 gtttaggagc caacctctta ttataatgac caccttttga catccactaa acgctaacac
     1381 cgtaatacgg ccttttcaag aaatacaact ggacaagact aacccaatta tgacatccgc
     1441 tgggaagtac ttcctctata tgggtaatgc cataggttaa gtaggtagga acctctcctt
     1501 aaaaacagtc agccctgagt tcaggacagg cttgctggtg ggctgatagt ggcaacgagc
     1561 aagtcacgag caagactcgt gacctacccg gttggattgc tacctgtttc ataaatcaaa
     1621 tttcataagg tctccgctac attttgaatt cgtaacaata cacaaattgt aacggaaggc
     1681 tttggccttc catagaacag tatctttggc cattgcaatg acagtggatt gttactgaag
     1741 tattctcctt tcatgaaaat cagggctgca atcattgtgg tgaaagactg gaaagaaagg
     1801 gcgtttctta gagcggagct tattgaagag ggagtaagaa catttgctgt tgaacggatg
     1861 ggcgaggctg aggagtggct agccgatcca gagatgctgc caataataat tatctacgac
     1921 actaataatc aggacagtcc ctctcgggat cttatgcgtc ttagtgaatt aattacacac
     1981 ataccagttt tgattattac atccttttta gaaaaagggg caagcaacct tgaagaactt
     2041 gggtttaaac acataatcca aagacctata agaattggag atgtagttaa gtacgttaaa
     2101 gagatattga aagggactaa aagatgaact cttcggggac cagcatcaca ttacggggtt
     2161 agaaaacccc gcctatcggt gtattttgat tctacctggg atgtgcaaga tgctacatcc
     2221 caacctaagt ggataggcgg gactttccag tcccgcaatg gcaagcgagg ttcaaaaacc
     2281 ccgcctatcg atatgcttaa attctactaa gcgatccctg ccgggatgga gggggtgaaa
     2341 attacatttt cctcttttat aatgtgttaa tgaagaaccc tacagcaaag gtaccgacat
     2401 attaaaaaga caatactttt tgtcattgcg aggagtgaaa cgacgaagca atccaaagat
     2461 gggattgctt cgccttcggc tcgcaatgac aggcaagcat tgaattgctt cgcggagttt
     2521 acactgagca cttcggtttc ctcagtagag gctccgtgaa tgtgaccgca atgacagtgg
     2581 aaacctcgaa gcaagctttg gatattgtcg agttaaattt agataagaca ctatgcacag
     2641 taataatgaa aaatctggaa gacccgaaga taactgtacc ggcctagctg tttttacctt
     2701 tctatttgtc tgggctacta tatttgaact tccagtaaca gcacccctac ctcaaaattt
     2761 ttctgctatt tctctaaaca acttaacaaa gcttgtgttt gtattcttgg ctatctgggc
     2821 tattttaaaa ccctcatcct tgtttaggtc catacccttt tttgcttaca cgctactagc
     2881 aatagtaata acactgcctg cggtacctaa tcatgttttc gttcagatta ttattttaac
     2941 tactattttg acatgtttta tcttttctat aatcaaagag agaagtaatt ttacaacaac
     3001 aaaattttac gaattattcg ccccgatatt acgactagaa ctcattataa tatatttttg
     3061 ggctgccttt cataagctta acaccggatt ccttaattat aagattagtt gcgcaactgt
     3121 acagatattt aatattaaag atatgattcc atttttccca actcctgatt ggtttataca
     3181 tatcaaccca tatttaaccc ttttaatcga gggtttgatt ccgatacttt taattattcc
     3241 gaaaacccgt atctatggct ttgtcttagg catttgtttt cactttattt taggttttca
     3301 ataccctggt tttactgtat ttgcttattc gttatattct ttatttatac cctcctctag
     3361 ctatgacaag attaaaacga aggtagcaga gctaatggat aggatttggg aagcactccc
     3421 aggaattagc aattacaaac attggaaaaa gacgatgttt ggaaacatta ctatcaatgt
     3481 tgttcttgtt ttgttgatat tctttattct tcgagttttt aaggcaggag gtcaaagaac
     3541 cttgcctcct gtcagtgagg ttggtcttta tctaattttt tgtacatttt tgggcattac
     3601 cttcatttat tttgtagtaa gaaggttgaa ggaattgagg atagatgaaa gactggtttt
     3661 tattccgcaa aagaaatggc ttttaatatt tccagctatt gtttttctga atggtctttt
     3721 accttacata ggattaaaaa atatacaggt atttgcgatg ttcagtaatc ttcaaacaga
     3781 gggtggaaag accaaccatc ttttgattcc atcctccttt caactattca acaatctaga
     3841 agaccttgtt tcaataaagc gctcaaataa taaaatgctt aatcagtttt ctcaaggtgt
     3901 tttcagaaag ataaagggtg ttacaaccgt tttaatcccg aaatcttatg tcgaatacat
     3961 gaaggataaa aatgagaatt ttaaaataat attaaagtat aagattccct ttgtgatgct
     4021 ccagaatata gttactcgag gaacacagct tggcgttaag aacatcgaac tggagtatga
     4081 gaggggaggt gaaaccttta atgccaagaa tgccgagctt gaccctcttc tgaataacgc
     4141 ctctgctttt cagataatat ttctaccaca gagggcagtt ccagacgatg aaagaggact
     4201 ttgcatgtgg tgagaatgaa aattgaaata acgatacaca ccacaaacga taggcgggac
     4261 tttccagtcc cgtaatggca agcggggttg gaaaaccccg cctatagtaa tcgcgagagc
     4321 cgaaggcaaa gcacaatggc gattacgatt gcatataagc ctcaatttag ataactttaa
     4381 ctagtgcaac accataattt gagaatttca aaaatcttga gtaaaattct aggcttcatg
     4441 aaaagaaatc gtttgctcat tatcattctt ttacctttaa tttacctttc gtccttgtcc
     4501 ccaaaccccc aagagtctac gcaaaatcag agcggagacc ccttaaaggt gcttccttcg
     4561 gtgatgcagc atataaaaag gcactacgct aacaaatcag ctatagaccc aaaagctatg
     4621 ttaatctcag ggcttggaag gcttgagaga aacctggatg aagtactggt tgaccttcca
     4681 gacggagaaa acgggacctc attcagggtt caggtaatta atgatgaaaa aacattcgat
     4741 atgaacgggg ttaacgatct agacagccta acacagaaaa ttgaggaggt atttaagttt
     4801 gtaacacccc ggttggtctc aagagatctt acaccagatg aaatcgaata tgccgtgata
     4861 gacgagatgc ttaataaact tgatcgtcac tcagggataa tcacgccaca gatctaccgt
     4921 gaatttatga ttgagactga gggaagcttc ggcgggcttg ggatagtaat aggcattaga
     4981 gatggtcagc ttacggtaat tgcccctatt gagggaacac ctgcatataa tgccggtata
     5041 aaaccgaatg ataaaatagt tcaaattgaa tatgaatcca cagtaaacat gtctcttatt
     5101 gaggcggtcg gaaaattgag aggccccaag ggcacaaatg ttaacattta tataatgaga
     5161 gaaggttttc cagattcaaa gaaattctca attgtaaggg ctacgattaa gatcgaaagc
     5221 gttgaggctt atagcctagg agatggaatt ggatatataa ggataaggga ctttcagaga
     5281 aacacccttg acagcattaa ggctagttta agaagcctga agaaaaatgg aagccttaat
     5341 gggctcatac tagaccttag gggaaatcca ggcgggcttc tagatcaggc ggaaaggatt
     5401 tcaaacctct tcttaaaaag cgggattata gttactacaa gctcaggaga ttctaaaaag
     5461 ccatacaagg caacaccaga ggattacgaa tatggcggaa gagtggtagt tctggtagac
     5521 tcaggaagcg caagcgcatc agagatagtt gcaggggcat taaagaacaa cgaaagagca
     5581 attgtggtgg gtgatacgac ctttggcaag ggctccgtgc agcagatttt tgaccttaac
     5641 gacggttccg ctcttaaact cactattgct cagtatctca cacctgggga tatatctatt
     5701 caagatatag ggattacccc tgatattcaa atttcccccg tgattgtctc caaagatgcc
     5761 tacaacacct catctgaaaa tcacctaata aatgaattat tgaacgggga gaaaaaagga
     5821 aagttagaaa ataaggaaaa gccaatctac accattttct accttgagac ccaaaaagat
     5881 actcaaaata agggcgaaga aacccctgat gaagccctaa gccgtgagga aaagcttaag
     5941 aatattgaga acgacttctc cgtagccttt gctaaggata ttcttctaca atcaaaatcc
     6001 cattcaagaa aagaaatact gaatcaggtc caaaccggaa tcaaagaaat atcagaaaac
     6061 gaagaaagga agattgtggg gaaatggcag agtgaggggg ttgattggtc aatcggtaaa
     6121 agggggtctg gagtaccggc tctatccata aaagtagctc cctctcccct aaaagccaag
     6181 gctggagaaa agctcgaagt aaaagctcaa ttagaaaaca caggccagat accactatat
     6241 aggttaaagg ctgtaacaga atcggataat ccaatattta atggaaagga atttatcttt
     6301 gggaagctcg gactgggtga gaaaatggag tctgccgtaa cgtttgaaat cccgaagggg
     6361 attctccaga gagaagatac aattactttg aagtttgttg acttttataa atccgatcta
     6421 ccaggattta catttaatgc gcaaattgag gagcttccaa gacctgcatt cgcctttaac
     6481 tacgagattg ttgacgacgg aagaatggat tcatccggta atggaaatgg tattcctgaa
     6541 actggagaat caatagggct tcttgtaaag gtaaaaaaca taggaagtgg atcatctgaa
     6601 aagggcatct tcacacttaa gaatctctcc ggcgattcta tatttttaca aaagggaaga
     6661 ttcgagtata aagacctgca tccaggtgaa gtgaaagatg ccccgtttat attcgaagtt
     6721 aaaaagccgg tacagcaaat cgagatggaa tttgggatct tggacgatgt gttcagggaa
     6781 gagcttaggg acaaatttcg tttgactcac aaggaagaaa atagaaatca ggttttcaaa
     6841 gaacctccat ctataagctt ctccaacctt tctcattcaa ccaaatcaca agagatcgct
     6901 ctaaatggac ttgtaaagga ccaaaacggc ctagaactcg taagtgtctt tgtaggagat
     6961 gataaggtaa tcctgctccc ttcgtctacc aaagaggttc ctttgtctat gaacgtaaaa
     7021 ctcaataagg gagttaactt gataaccgtc cttgcaaggg ataaagatgg actgacctcc
     7081 agggaaagcg ttgtggtaag atttggtgaa tgacaaaatt catcctgggt cgagtcctaa
     7141 gcggggcatt tgttgtatta atagtcgcta cactaacctt ttttctacta aggctccttc
     7201 ccggcggtcc ctttgataca gaaaaaaagg tcccacccga gattcgaaag aatatcgaag
     7261 agaaatatag gcttaatgag cccctatgga agcaatacta tatatatata agagacctag
     7321 cacacggaga ttttggccca tcgtataagt atatcgatcg gacggttagg gacataatta
     7381 aggaaaccct tcccgtttcg gtaaaacttg gaactgcggc tcttttaatc tctttaacaa
     7441 tcggtacagg tatagggatc ttttcagccg ctcgtccaag aggcttattt gacatccttt
     7501 cactctcttt tgctacagcc cttgtgtcag tgccaagctt tgtggtaggt gcggcgttga
     7561 tttacatatt ttccgttgag cttgactggt ttccagccgc tctatggggg aggcctgaga
     7621 attatgtgct tcctgcattt acactggcgg caggacctac agcctatctt gcccgtctta
     7681 caagggcaag catgcttgag gtttctcaag ctacatttgt gagaacagca tgggcaaagg
     7741 gcctaggtga attcaaagtg attttaaaac acatctttag aaatgcgctt atccccgttg
     7801 ttacagtttt aggtcccatc acagcctttt tagtaacagg gtcatttgta gttgagcaca
     7861 ttttttcgat tccaggaatg ggaagattct ttgtatttgc ggtgtcgaat agggattacc
     7921 cacttgtcat ggggattacg attgtataca cagtgatcct tgtaatcgct aacctattgg
     7981 ttgatatatg ctatgtaata ttagatccgc ggataaagtt tggaaagtag tagaggcgta
     8041 tcactatacg ctcctatagc ttgctgacta cttgtttgtt tgaaccaata taaatgaaaa
     8101 acatcaaact ggtccttgaa tacgacggca ctaattacct gggatggcag aagcaacccc
     8161 atggaattac aattcaagag gttatagaaa atacattaaa taagattact agtgagacaa
     8221 tcacagtgat tggatcagga agaaccgatt ctggtgttca tgcgctcgcc caggtggcta
     8281 actttaagac agaaatcaga atgacgtctc tacaattcca aaaagcgctt aactcagttc
     8341 ttccgaagga cattgttgta aaagaggtca gtgaggttga tataaacttc catgcgcaac
     8401 ttgacgttaa gagcaaaaca tatctatata aaatacttaa ccgcccatac ccctcagccc
     8461 ttgagagaaa cagggtatgg tttctccctg aatcattaga tgtgcctaag atggccgagg
     8521 ccgctaggtt gctcggtggc gaacatgact ttcgcggatt cgctttatct aatacaacaa
     8581 aaacgactgt gcgagaggtt atgaatgtaa acctcaaaaa gaaacctgac ggactagttg
     8641 aatttgagat tgaatcaacg ggattcttaa gaggaatggt tcgcctgatt gtcgggacgc
     8701 ttgtccaggt aggaaaaggg aaaatcgcac ctgaggattt tagagaaatt ttagaatctg
     8761 gagagaaaac taatctcgta ttttctgccc caccatcggg attatatctt aaggaggtac
     8821 ggtattgata aatctaataa tccaacgaga aggctttcgt aatataaacc ttagagaagc
     8881 caaacagcag gagtggcata gttacg
//
LOCUS       DATDRL010000021         5391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3119668, whole genome shotgun sequence.
ACCESSION   DATDRL010000021 DATDRL010000000
VERSION     DATDRL010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3119668"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VI935_00890"
     CDS             <1..625
                     /locus_tag="VI935_00890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HLE24186.1"
                     /translation="RKINLILTARSYTQLSYPRLSKLYEKASYLEREKINGSFVECGV
                     WNGGSAGIIASVARYNKNRHIWLFDSWEGLPKPTEDDISYIGEPGQEGMSLGYEEKVK
                     ELLFKKLKLNSSKVYLVRGWFNNTIPSHKKDIGEIALLHLDCDWYESVKLCLEELYDN
                     VINGGFIFIDDYGHWKGCRRAVDEFVEERNLRIELIKIDYTGAYFQK"
     gene            894..2267
                     /locus_tag="VI935_00895"
     CDS             894..2267
                     /locus_tag="VI935_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HLE24187.1"
                     /translation="MNLSKLIMRSEALIRGKVRQDSLRVTLMWNAGGTFALKVASTAL
                     LFLISLLLARLLGATGYGAYVNAMAWISLLSVLSMMGLDKLVVREIAIYNTRSAWDLM
                     RGLLRWTNLLGFFVSLWFALLTGIIIWILRDHLELQMRSTLWIALIMLPLLVLIRLRQ
                     AAMRGLQHVVFGQLPEMLIMPIVFVVLFGGIYLIFRDTLSASATMGAQVVAAGIAILI
                     GARQLKISLPQSAKQAPPTYQGWIWVRGILPLLFIDSMMIISAQTDIIMLGAMKGAET
                     VGIYAVAKKGAGLITFVLLAVNMALAPNIASLYTAGDMDKLQRVVTKSARAILLGSLP
                     IALALIAFGHWFLLLFFGQDFTQGEKALAILSVGQLINTATGSVGLLLIMTGYERDAA
                     IGVGSGTVLNVMLNAILIPRWGLTGAAAATASSMIVLNILLAIWVYKRLGIHSTALGE
                     IGLWRQA"
     gene            2264..3181
                     /locus_tag="VI935_00900"
     CDS             2264..3181
                     /locus_tag="VI935_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HLE24188.1"
                     /translation="MRRPDFFIVGAPKCGTTAMQDYLRQHPEIFMPEAKPPLFRGKEL
                     HFFGSDLKVNHAGLTEEEYLSYFQEAKNEKRVGETTVWYLYSKRAAYEIKEFNPSASI
                     IIMLRNPVDMLYSFHSQLLYNGDEYIEDFKTALDVEPDRKLGLRLPKQGLVTAERLFY
                     REVGRYPEQVKRYFDVFSREKVHVIIFDDFKTDIAKVYKETLRFLGVNEDYQPAFRII
                     NPNKGVRNKFLRSLLRNPPQIAISFAKVVMPQPLRQGLIEALTRYNIRYEPRPPIDRE
                     LRKRLQTEFASEVERLGELLGRDLSHWSN"
     gene            3275..3976
                     /locus_tag="VI935_00905"
     CDS             3275..3976
                     /locus_tag="VI935_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLE24189.1"
                     /translation="MEYGPIKYKDGIPLINKRKTVSILANRLFLDAMKEVVNHAKGEL
                     LDVGCGESPFYPLFLQRINSYVKTDILTSPIKPDIYSEIIALPFKAESFDTILCTHVL
                     EHVFEPFCAFNEMKRVLRSRGVIIIGTPLIYWIHEAPVDYFRYTRYFYQKVAEIYDFE
                     ILYLNEIGGIMNVLVDMVSKGIILVSLKGNYLGFLKHVSFLIQDIGWVIDKAFKKDFA
                     ASIINLGYVVAFRKR"
     gene            4070..4825
                     /locus_tag="VI935_00910"
     CDS             4070..4825
                     /locus_tag="VI935_00910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLE24190.1"
                     /translation="MSYEPKSYWEERLGQSFSLKHTGHIGFSLVYNKWSYKAKARALE
                     NALSDLKINCKGKAVLDIGCGTGFWVEFYKSKGAGSIVGVDITSISVEKLKEKYPQFE
                     FCELDIGGEEINIDGRFDIVNVFDVLFHIKDKLKFGKAMSNIAHLSKKGSYIFITDSL
                     IDRSEAEYVTFRSLSTYEEEFKRNSLRLIGVTPLYYLLNSTYPFFLRGIKFAFLLTKF
                     NIDDIMAPILYYLDKVLLSLERSHFKLIICVKE"
     gene            4822..>5391
                     /locus_tag="VI935_00915"
     CDS             4822..>5391
                     /locus_tag="VI935_00915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE24191.1"
                     /translation="MKVALFLSNFPTLPETFILNQIIGLINSGINFDIYASANPDEGK
                     VHAQVEKYKLLERVSYSDSIAPLFKRLTKGAKIVLMNFYKNPLAYTEAIKSRSLRDIF
                     YVNDFIANKSKRSYDLIHAHYGSNGLIGVILKEAGVFRGKLITSFHGYDFSKLINERG
                     SETYKELFRIGDLFTANTNFTKGKLIELGC"
BASE COUNT         1647 a          944 c         1186 g         1614 t
ORIGIN      
        1 acgtaaaata aatttaattc ttactgccag gtcttacacg cagttgtctt atccaagatt
       61 atctaaactt tatgaaaaag cctcttattt agaaagagaa aaaatcaatg gtagttttgt
      121 agaatgcggt gtatggaatg gtggcagtgc aggcataatc gcttcggttg ctagatataa
      181 caagaataga cacatttggc tttttgattc ttgggaagga ttacccaaac caaccgaaga
      241 cgatatttca tatatagggg aaccgggtca agaaggaatg tcattaggat atgaggaaaa
      301 ggttaaagaa ttattattta aaaagctcaa attgaatagt agcaaggtct atcttgtaag
      361 gggttggttt aacaatacta ttccttctca caagaaagac attggcgaga tcgctcttct
      421 acatctagat tgtgattggt atgaatcagt aaagctatgt ttggaggagt tatatgataa
      481 tgtaattaat ggtggtttta ttttcataga cgactatgga cattggaagg gttgtagaag
      541 agctgttgat gagttcgtcg aagagagaaa tctaaggata gaattgatta agattgatta
      601 taccggagct tactttcaaa aatgaataaa ataaaatccg tccctcgcaa cactcactcg
      661 cttacgctag ttcagggtta tagcgctgtt taacagaaga taaagaggaa cgaaaaggac
      721 tttacacaaa ggcgggggca gagttgatca aagattttaa ggggactgga gaccagcacg
      781 agccaccaga gcatctggat atggggtaga ctcctcagac ctgattacac agagaagacc
      841 gccatctgat tctgtcgtac ctgcataagg aagggatact tggagagcaa tcggtgaact
      901 taagtaaatt aatcatgcgc agtgaagcgc tcatccgcgg taaagttaga caggatagtt
      961 tacgcgttac ccttatgtgg aatgcaggag gcacgttcgc tctcaaagta gcttctacag
     1021 cactgttgtt tcttataagt cttctattag cgcgcctgtt aggtgcgaca ggatatggtg
     1081 cttatgtcaa cgccatggct tggatcagct tgctaagcgt tctatccatg atgggattag
     1141 ataagctggt tgttcgggaa atagcaatat acaacactcg atcggcgtgg gacttaatgc
     1201 gtggtctcct acgttggaca aatctcttag gattcttcgt ctcgctgtgg tttgcactgc
     1261 taacgggaat tattatctgg atattaaggg atcatctgga attacagatg aggtccaccc
     1321 tttggattgc tctgattatg ctacctttac tggtcctcat acgtcttaga caagcggcta
     1381 tgaggggact tcagcacgtt gtgttcggac aattaccaga gatgctcatt atgcctatcg
     1441 tctttgtcgt attatttggc ggtatatacc tgatttttag ggatacacta agtgcgtcag
     1501 caactatggg agcgcaggta gtggctgccg ggattgctat ccttatagga gcaagacaac
     1561 taaaaataag cctaccgcag tctgccaaac aagcacctcc tacctatcaa ggctggatat
     1621 gggtacgtgg tattttacca ctgctattta tagattctat gatgattatt agtgctcaga
     1681 ccgatatcat tatgcttggg gcgatgaaag gggcggaaac ggttggtatt tatgccgttg
     1741 ccaaaaaagg agcgggactt attacttttg tacttcttgc cgtcaatatg gcgctggcgc
     1801 ccaacattgc cagtctatat acagcaggtg atatggacaa actgcagcgt gtagttacaa
     1861 aaagcgcaag ggcgatctta ttgggctctc tgcccatcgc tcttgcccta attgcattcg
     1921 gtcactggtt cttgttactt ttcttcggtc aggatttcac ccaaggtgaa aaagcccttg
     1981 caattttgag cgtcggccag cttataaaca ctgctacggg atcggtcggt ttattactta
     2041 tcatgactgg gtacgaacgt gatgctgcta ttggtgttgg gagcggtacg gtgttgaacg
     2101 tcatgcttaa tgctattctt atacccaggt ggggactgac gggtgcagcc gctgctacgg
     2161 caagtagcat gatagttttg aatatcttgt tagcaatatg ggtgtataag aggcttggaa
     2221 ttcattcaac agctctgggg gaaataggtc tgtggaggca agcatgagga ggccagactt
     2281 tttcatcgta ggtgctccaa agtgtggcac cactgccatg caggattatc tcagacagca
     2341 cccggaaatc tttatgcctg aagcgaaacc gcctctcttt cgcggaaaag aacttcattt
     2401 ctttgggagt gaccttaagg ttaatcatgc agggcttact gaagaagagt atctttctta
     2461 tttccaagag gcaaagaatg agaaacgagt cggtgaaacg acagtttggt atctgtactc
     2521 aaaacgtgct gcttatgaaa ttaaggagtt caatccctct gcaagcatca tcattatgct
     2581 caggaaccct gtggacatgc tctattcttt ccactcgcaa cttctttata acggtgatga
     2641 gtatattgag gattttaaaa ccgccttgga tgtagagcca gaccgaaaac ttggtctgcg
     2701 tctaccaaaa caggggttag tcactgctga acgtttgttt tatagagaag ttgggcgata
     2761 tcctgaacaa gttaaaagat actttgatgt ttttagcagg gaaaaggttc atgttattat
     2821 tttcgacgac ttcaaaactg acatagctaa ggtttataaa gaaacactcc gctttttagg
     2881 tgtaaacgaa gattatcaac cggcttttag aattatcaat cccaataagg gagtacgtaa
     2941 caagtttctg aggagtttat taagaaaccc gccacaaatc gctatatcgt ttgctaaggt
     3001 agtgatgccc cagccattgc gccaaggctt aatcgaagcg ctcacgagat ataatatcag
     3061 atatgagcca cgaccaccca ttgatcggga attaaggaag cgcctacaaa cagagtttgc
     3121 ttcggaagta gaaagacttg gtgaactatt aggaagagat cttagtcatt ggagtaatta
     3181 actataaaat ttttgagaac ttatgctcag ggtgcgactt atgaatagtg taacatctga
     3241 ttttatttta aaagaaaata aatgattaac gactatggaa tacggaccta ttaaatataa
     3301 agacgggata ccccttatta ataaaagaaa gactgtctcg atcctagcca atagactttt
     3361 cttggatgca atgaaagaag tcgtaaatca tgctaaagga gaactactcg atgtgggttg
     3421 cggtgaatct ccattttatc ctttgttttt acaaaggatt aatagctatg ttaaaacaga
     3481 tatacttacc tctcccataa agccggatat atactcagag ataattgccc ttccttttaa
     3541 agctgaaagc tttgatacta ttctttgtac tcacgtttta gagcatgttt tcgagccatt
     3601 ttgcgctttt aatgaaatga agcgcgttct tcgatcgaga ggagtgatta taatagggac
     3661 tcctcttatc tactggatac acgaagctcc tgttgattac tttagatata caagatactt
     3721 ttaccagaaa gttgctgaaa tatatgactt tgaaatcctt tacttgaacg aaataggagg
     3781 tattatgaac gtcctcgttg atatggtatc taagggaata attcttgtct ccttaaaggg
     3841 taactacctg ggcttcttaa agcatgtctc tttccttata caagatattg gatgggttat
     3901 tgataaagcc tttaaaaagg actttgctgc ctctattatc aacttaggtt acgttgtggc
     3961 ttttagaaaa agatgattag cataaaagat ttctgttaaa agtacttgaa atggagaaaa
     4021 gctattaaaa ttatcaagct aacatgactt acgaatttat tgagctaaaa tgagttatga
     4081 accaaagtcc tattgggaag aaagactagg gcagagtttc tcattaaaac ataccggtca
     4141 catcggcttt agccttgttt ataataaatg gtcatacaaa gcaaaagctc gggcccttga
     4201 aaatgcctta agtgatttaa agataaattg caagggcaaa gctgtcttag acattggatg
     4261 tggaacgggc ttttgggttg aattctataa atcaaaagga gctggttcaa tagtcggcgt
     4321 tgatataacc agcatcagtg tcgaaaaatt aaaagagaaa tatccacaat ttgaattctg
     4381 tgagctggat ataggaggtg aggagataaa catagatgga agatttgata tagttaatgt
     4441 ttttgatgtc ttgtttcata taaaagataa gctaaaattt ggaaaggcga tgtctaatat
     4501 cgcacattta tctaaaaaag gttcttatat cttcataacc gattcgttaa tcgacaggag
     4561 tgaagccgag tacgttacct ttagaagctt aagcacatac gaggaggaat ttaagagaaa
     4621 tagtttaagg ctaataggag ttacaccact ttattatcta ttgaacagta cttatccttt
     4681 ttttttgaga ggaatcaaat ttgcttttct tctaacaaaa tttaatatag acgatataat
     4741 ggctcccatc ctttattacc ttgataaggt tcttttatca cttgagagat ctcattttaa
     4801 gcttattatc tgtgtaaaag aatgaaggta gctcttttcc tttcaaactt ccccaccctc
     4861 cccgaaacat ttatcttaaa ccagataatc gggcttataa attcgggaat taactttgat
     4921 atttacgctt cggctaatcc cgatgaaggc aaagtccatg ctcaggtaga gaaatataag
     4981 ttgctagaaa gagttagtta ttccgattca atcgctccac tatttaagag attaaccaaa
     5041 ggtgcgaaga tagttttaat gaatttttac aaaaatcctc tagcatacac cgaagcaata
     5101 aaaagccggt cgcttagaga catattttat gtcaacgact tcatagctaa taaatcgaaa
     5161 agatcgtatg acttaataca cgcccactac ggttctaatg gacttatagg agtgatttta
     5221 aaggaagcag gtgtttttag agggaagctt attacttcct ttcacggata cgattttagc
     5281 aaattaatta atgaaagggg gagtgagacc tacaaggaac tttttagaat aggagacctt
     5341 tttaccgcta atactaattt tactaaaggg aaattgattg aattagggtg t
//
LOCUS       DATDRL010000022         4263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1805283, whole genome shotgun sequence.
ACCESSION   DATDRL010000022 DATDRL010000000
VERSION     DATDRL010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805283"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VI935_00920"
     CDS             <1..475
                     /locus_tag="VI935_00920"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HLE24192.1"
                     /translation="ASSAFVTQKEATLKKGESISVNHYTLRFDELNQYTTEMKHVTAA
                     KLSVFNSERNLGVLVPEKNVYKYEGNRDIDNETEVALRSTFKDDLYVILTNFDDNGTA
                     SFRVLINPMVNWIWAGGIVLALGAIVIMWPSARERREEAVVRYSVETSEEGAAKV"
     gene            697..1206
                     /locus_tag="VI935_00925"
     CDS             697..1206
                     /locus_tag="VI935_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HLE24193.1"
                     /translation="MEKLFILILAIAAAIFVALPFFRKRPVEIPQDINSSNTLEHKLK
                     RLNSEKESLYAALKELDFDYSMGKLSKEDYDELEKKYKAEALAVLKQIDNIQLKTNKL
                     NLEEEIEKEIKAVRESKLTDDEVIEKEILKARQSNVKVTSGLVCSKCGNEYKSSDRFC
                     SNCGARVYE"
     gene            1199..2689
                     /locus_tag="VI935_00930"
     CDS             1199..2689
                     /locus_tag="VI935_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLE24194.1"
                     /translation="MSRVIFLSVRTFLILLCMIISSYKEIQAQNKDTTQLSGKELFIQ
                     YRCVRCHTIGRGRFVGPDLEGVSSRYSKDDIKKWIENPQEIYQSKGKTPLNEGYPPMP
                     PLNIPPKEAELIAQYLLTVRTSPLSKEGGVIRGKVVNNSTEKPAKELEVILTAYLGDK
                     ATEEKKVRTNTDGIFEFKNLPWDRSYSISLRYEGGEYLTDKLVFYPDEDTKSLDLPIY
                     EPTDIDKDITVDISHVIVQTSENSLSVAELTVFNNGGKRTYIGAKEIKDDMRETLRFS
                     LPPGASNIELINGLNSESVVPTNNGFVDTSSVLPGIKRVVYAYTLPYKSGREIILKTV
                     DYQTDSFVLLISDSGVKVKVQGLTGSETVKIPDQGRFLQWSGTKIPSGTKIRVDIGES
                     RLLGKDSLKFITFGVLAILVGIGVLYSYVMKSRGKSKTKNISLPTASGIVVRKDLEEE
                     RRLLIHEIAKLDDSYGAKEITEEEYMKMRLKKKQRLVEITRKIKKE"
     gene            complement(2722..3444)
                     /locus_tag="VI935_00935"
     CDS             complement(2722..3444)
                     /locus_tag="VI935_00935"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HLE24195.1"
                     /translation="MPRFPISQNQIKNNLAIVSGSDYRHIVKVLRLKPGNEITLFDEK
                     GVEHFGQITEIRTKEIRITIAESKRIETKPHLNITLLQGIPKGDKMDFIVEKATELGV
                     KTIVPVVTERSQIRKTKRIERWRRIALESSKQSRRMMPPDILDLMSFDNAIYSNDKGE
                     LYLIFYEKCKDNIKDIIKHAGKATENIKIFIGPEGGFSENEVNAAKEKGFIPIGLGPR
                     ILRTETAGIVAVAVLQFLFGDI"
     gene            complement(3602..>4263)
                     /locus_tag="VI935_00940"
     CDS             complement(3602..>4263)
                     /locus_tag="VI935_00940"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HLE24196.1"
                     /translation="NDVDRAIEMLHIYNWIIFTSVNGVEFFFKRLRETKKDIRELKGI
                     NISTIGEKTAKAVENYGLTVNMIPDEYRAEGIIESFEKSKIKGKKILIPRAKDARETL
                     PHELQKMGAEIHVVTVYETKKPELKKVKEIKEMLNRGAIDVVTFASSGTVRNFLSIIP
                     KYKELLLKSTIACIGPITAKPLRDIGIEPQIVCKKYTIEELTREIIDYFAAIQPRIEK
                     K"
BASE COUNT         1344 a          721 c          844 g         1354 t
ORIGIN      
        1 tgcatcatca gcctttgtaa cacagaagga ggctactctc aagaaaggag aatctattag
       61 tgttaatcat tatactctaa gattcgatga gcttaatcag tatacaactg agatgaaaca
      121 tgtaaccgcc gcgaagttat ccgtattcaa ttcggaaagg aatcttggtg ttttagtccc
      181 tgagaagaac gtttacaaat acgagggaaa tcgagatata gataacgaaa ccgaggtagc
      241 gctccgctcg actttcaaag acgaccttta tgttattctg acaaacttcg atgataatgg
      301 aacagcaagc tttagggttc ttataaaccc aatggttaat tggatttggg ctggagggat
      361 tgtgcttgct ctcggggcta ttgtgattat gtggccgagc gcgagagaaa gaagagagga
      421 agctgttgtc cgatacagtg tagaaacttc ggaggaaggc gctgcaaagg tttagaattt
      481 tacaaaggta aggaactccc tctgaggttt gatcattagt atgtcgcaaa actatcctta
      541 atttattttg tcgttgtaag gaaaagactc cctaacccac gcatgcgtaa aatgcaaaaa
      601 gcgtcattgc gagggacaaa gtcccgaagc aatctcaaaa gcgatcaagg gattgcttcg
      661 ccaaagctcg caatgacaat tagggaatga ataaaattgg aaaagttgtt tattttaata
      721 ttagcaatcg cagccgcgat ttttgtagca ttgccatttt ttaggaagag acctgtagag
      781 atcccccaag acataaatag ctcaaatacc ttggaacata agctaaaaag gcttaactct
      841 gaaaaagaat ccctatatgc tgcactcaag gagctagatt ttgattacag tatgggaaaa
      901 ctttctaaag aggattatga cgaacttgag aagaaataca aggcagaggc cttagctgtg
      961 cttaaacaaa tcgacaatat tcaattgaaa accaataagt tgaatttaga agaagaaata
     1021 gagaaggaaa taaaagccgt tagagaatct aaattgactg atgacgaagt tattgaaaaa
     1081 gagattctca aagctcgtca atccaacgtt aaagtaactt caggattagt ctgctctaaa
     1141 tgcgggaatg aatataaatc tagtgataga ttctgttcta attgtggagc gagagtttat
     1201 gagtagggtg atttttttaa gtgtaaggac tttcttgatt cttctatgca tgattatatc
     1261 ttcatacaag gagattcaag ctcagaataa ggatacaact caattaagtg ggaaagagct
     1321 ttttatacag taccgctgtg tgcgatgcca cacaattgga agaggaagat ttgtagggcc
     1381 ggatttagaa ggggttagca gtaggtatag taaagatgac ataaaaaaat ggatagaaaa
     1441 tccccaggaa atttatcaat ctaaggggaa gacgcctcta aatgaaggat acccacctat
     1501 gcctccatta aatattcctc caaaggaggc agaattaatt gctcaatatc ttctcacggt
     1561 aagaacttct cctttatcaa aagaaggtgg tgtgataaga gggaaggtcg tgaataatag
     1621 cactgaaaag cctgcgaaag agcttgaggt tattcttaca gcttatctag gcgataaagc
     1681 aactgaagag aaaaaggtaa gaacaaacac agatggaatc tttgagttta agaatcttcc
     1741 atgggatcgt agctattcta tttcgcttag atatgaagga ggagaatatt taacagacaa
     1801 gcttgttttt tatcctgatg aagatacaaa atccctcgat ttgccaattt atgaacctac
     1861 agatattgac aaggatataa cggtagatat aagtcatgtg attgtacaga cttctgaaaa
     1921 ttcactttca gttgctgagt taacggtttt taataacgga ggcaaaagaa catacatagg
     1981 tgcaaaagag ataaaggatg acatgagaga aaccctcagg tttagtcttc cacccggggc
     2041 gagtaacatt gagcttatta atggacttaa cagtgagagt gttgtaccga ctaataacgg
     2101 ttttgtggat acttctagcg ttttgccagg gattaaaaga gtagtatatg catacacgct
     2161 cccatataaa tcgggtagag agattatctt gaaaacggta gattatcaaa ctgacagctt
     2221 tgtccttcta atttcagatt ctggagttaa agtaaaagtg caggggctaa ccggatctga
     2281 aactgtaaag atacctgatc aaggtcggtt tctccaatgg tctgggacta aaattccttc
     2341 tggaactaaa ataagggtag atatcggcga atcaaggttg cttggtaagg attctttaaa
     2401 atttataaca tttggagtat tggcgatttt agtaggtatt ggagttttat attcctatgt
     2461 aatgaaatct aggggtaaaa gcaaaacaaa gaatatatcc cttccaactg cctcaggcat
     2521 agttgtaaga aaggatttag aggaagagag gagattactc atccatgaga ttgcaaaact
     2581 cgatgatagc tatggggcta aggaaatcac tgaagaagaa tatatgaaga tgcggttaaa
     2641 gaagaaacaa aggctggttg agattactcg aaaaattaaa aaggaatgaa gtctcagcaa
     2701 atgactattt gtaaaaattt gctaaatatc tccaaataga aactgtaaaa ccgcaactgc
     2761 aactatccct gccgtctcag ttctgagtat tcttggacca agtcctattg gaataaagcc
     2821 tttttcttta gcggcattta cctcgttttc tgaaaatcca ccttctggtc caatgaaaat
     2881 cttaatattt tctgtggctt taccggcatg tttaattata tctttaatat tatctttaca
     2941 tttctcgtaa aatataaggt ataactctcc tttatcatta gaataaatag cattatcaaa
     3001 actcatcaaa tcaagaatat caggtggcat cattcttcta gattgttttg aagattccag
     3061 tgcaattcta cgccaccttt cgattctttt agtttttcta atttgagatc tttcagtaac
     3121 tacaggcact atggttttta ctcctagttc tgtggctttc tcgacaataa aatccatttt
     3181 atctccctta ggtatgccct ggaggagcgt gatatttaaa tgaggtttag tttctattct
     3241 tttagactca gctattgtaa ttctaatttc tttagttcta atctctgtta tttgaccaaa
     3301 gtgttctact cctttttcat caaatagggt aatttcattt cctggtttaa gccttaaaac
     3361 ctttacaatg tgtctgtagt ctgatccaga gacaatcgct aggttgttct taatctgatt
     3421 ttggcttatt ggaaatctag gcattatagc tttagctgct agtcttgtaa cttttaacga
     3481 caaataagca ctgacttata ctgataaaat gatgagtgat acacgattca tgatgcatga
     3541 aatatatatc atcttgcatt gtgcatcctg tatcttgaac cctaactatt ttcattcgtg
     3601 atcatttttt ttcaattcgt ggctgaatag ctgcaaagta atctattatt tctcttgtaa
     3661 gttcttctat tgtgtatttt ttgcaaacaa tctgaggttc aatgcctata tccctaagtg
     3721 gtttggctgt aatgggtccg atacacgcaa ttgttgattt taaaagaagt tctttgtatt
     3781 tagggattat agataggaaa tttctcacag ttccggagct agcaaacgta acaacatcga
     3841 tggcgccgcg atttagcatt tcctttattt cctttacctt ttttagttca ggtttttttg
     3901 tttcataaac ggttacgaca tgaatctctg cacccatttt ttgaagctca tgaggcaggg
     3961 tttctcttgc atcttttgct cttggtataa gaatcttttt gccttttatt ttactctttt
     4021 caaaactttc aattattccc tcagctctgt actcatctgg aatcatgttt acggtgagtc
     4081 cgtaattttc aactgctttt gcggtttttt ctcctatcgt agaaatatta attcctttaa
     4141 gctctcttat atcctttttt gtttctctta gtcttttaaa aaagaactct actccattaa
     4201 cacttgtaaa aataatccag ttatatatgt gcaacatttc tattgctctg tctacgtcgt
     4261 tcc
//
LOCUS       DATDRL010000023         3610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3646399, whole genome shotgun sequence.
ACCESSION   DATDRL010000023 DATDRL010000000
VERSION     DATDRL010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3646399"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..600
                     /locus_tag="VI935_00945"
     CDS             184..600
                     /locus_tag="VI935_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24197.1"
                     /translation="MTNEPDDMARARRLLEEFERAGGLSKQDCFEEAIILLRDFSTEH
                     PNSEFSEIANNLRYTYTKILIEKLSSVSFSNYADWFATWEWTRRFSEEIEKVIIDKPP
                     LKDSFNKFEEFWKEKPFQDELNKSIQEELKKRGIKK"
     gene            660..929
                     /locus_tag="VI935_00950"
     CDS             660..929
                     /locus_tag="VI935_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24198.1"
                     /translation="MEVKKEILELCKMKIYNELGSLDDLPYELANTVANVYYRLFNFL
                     LYANALLDSNIPGSKFIKEGDVKTTIKRTKSFFKDLAEYSTTGFI"
     gene            1022..2302
                     /locus_tag="VI935_00955"
     CDS             1022..2302
                     /locus_tag="VI935_00955"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLE24199.1"
                     /translation="MKNQNEYEKRCKAIQLYEEGFGFNKVLQLVQRSRRWLSKWLKRF
                     KEHGINGLKDQSRVPKRIWRKTAKRLVKKILSIREELESHKTRRSAFSGIGAEVIHWE
                     LKQRKVHNIPAISTIANILSRHGKTSKVKPKRNSNNQPYPYFKAEKMGDLHQTDLVGP
                     RHLRGSKGVTRFYSFHTVDVAGHTAFTSQFTDKQTISLCKHLVETWRFMGIPTVSQMD
                     NEMAATGGGRYPYIISQVIRLHLLMGIHLVFIPQGEPGRNASIESFNALWQERAIHRH
                     NCPTLTVLRKTSEQFLQYYHYEKPHRSLTQKEHGTRFSGVLKDHLWKSLIHLPKGFSL
                     DRYIDSNGHLNLPIAKGKISFIRKVDSQGRIEINGSTYFIRRKLEGQYVATTIFTHRK
                     RLVVKQENRIIKSFSFPIDGHIINPLVIYKKRKT"
     gene            2440..2991
                     /locus_tag="VI935_00960"
     CDS             2440..2991
                     /locus_tag="VI935_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HLE24200.1"
                     /translation="MKALTIKHSEITRESLLMLAEDIRGAWIGIRIAACLLLLSGWKS
                     THVAELFGLTRWSVVKWIRKANMKGLEGIEDRVRLGRPSQFDNALKKGLDKALSKSPK
                     EFGIQRVRWDGVVVAEYLKRFHGIEIHVRHAQRWIRNLGYSLRQPIYRYVQATKEGVN
                     EFHEELKKTQLGDKKRRGKGNTI"
     gene            2954..3481
                     /locus_tag="VI935_00965"
     CDS             2954..3481
                     /locus_tag="VI935_00965"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HLE24201.1"
                     /translation="MIKSGGERVILFEDEAGFSLHPKLGRVWVKKGTQPEVDTRSQHQ
                     KRLNVFGWVEPINGLHGMLEWVKGNTDGFLEVLQQIKNRFKGKVIDLWVDRASWHRGK
                     RIEAFCMEYSQLRINYLPSYHPELNYQERLWKVMRYEETTNAFFEDIEQLTVSVFKRS
                     QRWKPKKIRSLCQLI"
BASE COUNT         1245 a          579 c          759 g         1027 t
ORIGIN      
        1 aaaaatgatg ctgaagatat caagaccgaa aggaatgatg ctgttcataa ttgtaaaatt
       61 tataataatg ctgacacatt agaaattata gaaaaaacca aaataattat gcaaaacatt
      121 tatgaaagac aatgacctgc ccatcagcaa gctggcgggc aaattcaaaa acattgtaag
      181 agtatgacta atgaaccaga tgatatggca agggcaagaa ggctattgga agaatttgaa
      241 agggctggag ggctatctaa acaagactgt tttgaggaag ctataatatt attaagagat
      301 ttttcaactg aacatcctaa ttccgaattt tcagaaatag caaataactt aagatatact
      361 tatacaaaaa tattaataga aaaacttagt tcagtatctt tttcaaatta tgctgattgg
      421 tttgccacat gggagtggac taggagattt tcagaagaaa ttgaaaaagt aataatagat
      481 aagccgccac tgaaagatag ttttaacaaa tttgaggaat tctggaaaga gaagccattt
      541 caagatgaac tcaataaaag tattcaagaa gaattaaaaa aaagaggcat taaaaaatga
      601 cgattgataa ctaatatcta atatcagaat ttagatttct aaaattcatt taaggacata
      661 tggaggtcaa gaaagaaatc ttagaattgt gtaaaatgaa aatttacaat gagttgggct
      721 cactggatga cctcccatat gaacttgcca atacagttgc aaatgtgtat tacagattgt
      781 ttaatttttt gttatatgct aatgcccttt tggattcaaa cattcctggg tcaaaattta
      841 taaaggaagg tgatgttaaa actacaataa aaagaacaaa atcattcttc aaggatcttg
      901 cagaatatag cactaccggg tttatctaat aacatcgtat acttaaaaaa tctaataaca
      961 tcgtaaacta atttacatcg tctaaacttc ttccaaaaac tttttggagg aggtaaaggc
     1021 gatgaagaat caaaatgaat atgaaaaacg ctgtaaggca atacaacttt atgaagaagg
     1081 attcggcttt aataaagtgc tccaactggt tcaaaggagc agaagatggc tttctaaatg
     1141 gctcaaacga tttaaagagc atgggattaa cggtcttaaa gatcaaagcc gtgtccccaa
     1201 aaggatatgg agaaagactg ccaagcgttt ggttaaaaag attctctcca tcagagaaga
     1261 attggagtct cataagactc gtcgctctgc cttctccggc attggagcag aggttatcca
     1321 ttgggaactt aaacaaagaa aggttcataa tattccagct atctctacta ttgctaatat
     1381 tctctctcgt cacggaaaga caagtaaagt taagccgaaa agaaatagca ataatcagcc
     1441 ttatccttac tttaaagcag aaaagatggg agacctccat cagacagacc ttgttggacc
     1501 aaggcacctc agaggcagca aaggtgttac cagattctat tctttccata ctgtggatgt
     1561 ggcagggcat acagccttta caagtcagtt tactgataaa caaaccatct ctctctgcaa
     1621 acatctggtt gagacatgga gatttatggg cattcccaca gtatcccaga tggataatga
     1681 gatggctgct acaggtggag ggcgttatcc ttatatcatt tctcaggtta ttcgcctgca
     1741 cttgctcatg gggatacatc tggtatttat cccacaggga gaacccggca ggaatgcctc
     1801 tattgagagt ttcaacgccc tctggcaaga aagagcgatt cataggcata actgccctac
     1861 ccttactgtc cttagaaaaa ccagcgaaca attcttgcaa tattatcatt atgagaaacc
     1921 tcacagaagt cttactcaaa aagaacatgg cacaagattc tctggagtgc ttaaagacca
     1981 cctctggaaa tcccttattc atctgccaaa gggttttagc cttgatagat acattgactc
     2041 taacggacac cttaatctcc ctattgcgaa gggaaaaatc tccttcatca gaaaagttga
     2101 ctctcagggg agaattgaga tcaatggttc tacctatttc atcaggagaa aacttgaggg
     2161 acaatatgtt gctactacta tctttactca tagaaaaaga ttggttgtta aacaagaaaa
     2221 tagaattatc aaatcttttt cttttccaat tgatggtcat attattaatc ctttggttat
     2281 ctataaaaag agaaaaactt aatttaagta tacgatgtta ttagatttct gagcattaag
     2341 aattagtata cgatgttatt atagtgaacg acaaaaataa gtagacatca tttgcgattt
     2401 attatatgct aattccaata taatttattg gaggtgcaga tgaaggcatt aaccataaaa
     2461 cattcagaaa taacgagaga atcgctactt atgttggcgg aagacattcg tggtgcatgg
     2521 ataggtatca gaatagcggc gtgtttattg ttgctttcag gttggaagtc tacccatgta
     2581 gcagaattat ttggtttgac tcgttggtct gtggttaagt ggattcgtaa ggcaaacatg
     2641 aaagggttgg aagggattga agatagagtg cgactaggaa ggccgtcaca gtttgacaat
     2701 gccttaaaaa aggggttaga taaagcctta tctaagtctc cgaaagaatt tggaatacaa
     2761 cgagttagat gggacggagt tgttgttgca gagtatttga agagatttca tggaatagaa
     2821 attcatgtgc gtcatgctca gagatggata cggaatttgg ggtattcatt acggcagccg
     2881 atatatcgtt atgtccaggc tacaaaagaa ggagtgaatg agtttcatga ggagttaaaa
     2941 aaaactcaat tgggtgataa aaagcggagg ggaaagggta atactatttg aagatgaggc
     3001 ggggttttcc ctacatccca aattagggag ggtgtgggta aagaaaggca ctcagcctga
     3061 agttgacact cgcagtcaac atcagaagcg gttgaatgta ttcggatggg tggaaccgat
     3121 caatggactg cacggtatgc tcgaatgggt taaagggaat accgatggtt ttttggaagt
     3181 acttcagcag ataaagaacc ggtttaaagg aaaagttatt gacttatggg tagacagggc
     3241 aagttggcat aggggaaaac gtattgaggc gttttgcatg gaatacagcc aactccggat
     3301 caattattta ccttcttatc atccagagtt gaattatcaa gagaggctgt ggaaagtaat
     3361 gagatatgaa gaaacaacaa atgctttttt tgaggatatt gagcaattaa ctgtttctgt
     3421 atttaaacga tctcaacgat ggaagcctaa aaagataagg tcattatgtc aacttattta
     3481 agtcgttcac tataactcct ctaatgccct ctcaaattga ctctttggaa gcttattagc
     3541 cagttcatct tctattgtag gcttccctgt tgatttgtat gttgtgtgtc tgtcaagata
     3601 tatacttata
//
LOCUS       DATDRL010000024         7499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3379932, whole genome shotgun sequence.
ACCESSION   DATDRL010000024 DATDRL010000000
VERSION     DATDRL010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7499
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3379932"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VI935_00970"
     CDS             <1..341
                     /locus_tag="VI935_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24202.1"
                     /translation="GWDKILNLRIFDHVAQRIVGLSFIQWAELFSSLLSGVFVFLGVF
                     YIKRSRLIAFQMFERSILVSLFLTNVFIFYQKQFGALSHLIFDILIIVALRYMIGREG
                     SVILKQPKVR"
     gene            complement(684..1058)
                     /locus_tag="VI935_00975"
     CDS             complement(684..1058)
                     /locus_tag="VI935_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24203.1"
                     /translation="MREIIFGLSIIIVSSCASYAPVVDPQSVKNEDRYYRDQAECKNI
                     ASQNTSTAKNVAKDTVIGGAVGAGTGALLGVITGSVTTGLAAGTVIGGVAGGAKGAYD
                     SDKNYETIYRNCMRGRGYNVLN"
     gene            complement(1138..1815)
                     /locus_tag="VI935_00980"
     CDS             complement(1138..1815)
                     /locus_tag="VI935_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24204.1"
                     /translation="MRKIFILPIFVLALITTYSSNVYAKDEPLSVKEFRQLYDSLLAG
                     KILVTETTQDGVIVKKERQFGKAVDVGDGEFDIPVSIVITKTKDGKLEQKTTINVLDH
                     INNLGGTAIVSEEFRKTTVEESGSEPKTTREIEFNGLFRVSKNDKGGFDVHNFGLVPS
                     VLVDEDSMNLAGSMVSYSCFTEKNSAKCILTIRDYKLGDYKPLVGFTLIEPAGGDVVE
                     VSEEIVP"
     gene            2305..3195
                     /locus_tag="VI935_00985"
     CDS             2305..3195
                     /locus_tag="VI935_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLE24205.1"
                     /translation="MEKEYWFVLIAAILYGTITAGGQFFVNQGLSLFEISLYRVLFIS
                     LLLLPIVLIKRELLIKRGMLLFFLVYGLIGAFLELSQFGGIALGVPVAIVVLLLYSQP
                     IWTIIIGRFMLNEKITLRKVLAVFIALSGVFIILKSWDIQSPKSLAGVISSLLGGIIL
                     SLWVIWGRMSGINKQHYVTTTFGWTAFSVIWLLLLWPIVNIFIREPTIIRISTDFPLR
                     YWFYFAIFGFIGGVIPHLFFYRGVQKVQAGVAGIILLLEPVSATVLADILFNQPIGLN
                     IFIGGALILVSNYLIIHKSE"
     gene            3626..4039
                     /locus_tag="VI935_00990"
     CDS             3626..4039
                     /locus_tag="VI935_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24206.1"
                     /translation="MKSIYKDNFVYLKNLTQLIYETLGEYQHLWFNLIKPVNPPLERE
                     YEDDEELPGIQKKGHLFLSELYRLCKEDQSKEFNMYEIGERLGFDEFETELIVENLAN
                     SELISHQKSSDEVSITPYGIMTIKGEIRIGYAPVH"
     gene            complement(4083..5219)
                     /locus_tag="VI935_00995"
     CDS             complement(4083..5219)
                     /locus_tag="VI935_00995"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLE24207.1"
                     /translation="MKFEEFILSNSVNRAIRDMGFSIPTPIQEEAIPHALSGRNIIGQ
                     AQTGTGKTVAFGIPIVERIDPLDKEVQAIVLTPTRELAVQVSKELGKIGKHKRITVLP
                     VYGGQPIGKQLDQLSKGVNIVVGTPGRVIDHIRRGTLSLKSVRIAVLDEADRMLDMGF
                     IDDMKFILAQIPGRCQIMLFSATIPSEISWLAKRYMKDPVEIMVSKDTLTVPETKQIF
                     FEVREMGKLGVLSKLIELERGGVFLVFRRTKAGVDDLTAALKARGYNAQALHGDCSQR
                     ERDRVMGLFRRGEVDIVVATDVAARGLDIDGITHVVNYDIPQDAESYVHRIGRTGRCG
                     KEGKAITFVTHWEYGELKRIQAFSKAKIERASIPTEVGYARLGS"
     gene            5566..5769
                     /locus_tag="VI935_01000"
     CDS             5566..5769
                     /locus_tag="VI935_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24208.1"
                     /translation="MNGTKGFYVGHDRAIYTRGIKQIRAQSPNLWTWIAKGGRMGQVR
                     DRLNQIVLHICDGLIIFSIDIQS"
     gene            6483..6677
                     /locus_tag="VI935_01005"
     CDS             6483..6677
                     /locus_tag="VI935_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24209.1"
                     /translation="MVSKQVKVGMEEAVVKVVGMEDEGVMAAVGTVDMVVDGEAVMVV
                     DGMVVVVVGMGTTQISFTST"
     gene            complement(6933..7094)
                     /locus_tag="VI935_01010"
     CDS             complement(6933..7094)
                     /locus_tag="VI935_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24210.1"
                     /translation="MGTKITRRQTPEEKELNKKLSELTSLEANTSSPTNLGQVSKYKF
                     FNGRVDNKV"
BASE COUNT         2204 a         1545 c         1396 g         2354 t
ORIGIN      
        1 ttggttggga taaaatactc aatcttcgaa tcttcgatca tgtagctcaa cgaatagttg
       61 gtctctcctt cattcaatgg gctgagcttt tttcatcgct tttatccggt gtatttgtat
      121 ttctcggcgt attttacata aaacggtcaa gactcatagc cttccaaatg tttgaacgct
      181 ctattctagt atctttattt ctcactaatg ttttcatatt ttatcaaaag cagtttgggg
      241 ccctatcgca tcttattttt gatatcttga ttatagtggc ccttagatac atgataggac
      301 gagaaggatc agtgatatta aaacaaccaa aagtaagata attaatgtaa gtgggatgga
      361 ataaatctgt tcagtggctt acaatagaat aaaaattgta ggaatctata aattaatgtg
      421 agcccgacgt aagagaataa ttgtagtgga atcctttagg attccgttaa cgtagatacg
      481 atggtctttc aattattaaa ctggtaacaa ataacccaac tgcgtatgca acgagtccct
      541 tcgtttcaac tcaagacagg cttgctcgtt gctggacgaa gaatggcccc acaaggttaa
      601 ttatagaatc ttcacaagtg gtgcacaatc aaatcttcat ggattttgag aatcaatgac
      661 ctgaaagttt tttaccaact gatttagttt agcacattat accccctccc cctcatgcag
      721 tttctataga ttgtctcata atttttatca ctgtcgtaag cgccctttgc tccaccagct
      781 actcctccta tgactgtccc tgctgcaaga cccgttgtga ctgagcctgt tatcactcca
      841 agaagagccc ctgttccagc tccgactgca cctccaatta cagtatcctt cgctacgttt
      901 ttagcagtgc tcgtgttttg gcttgctata tttttacact ccgcttgatc tctatagtac
      961 ctgtcttcgt tcttaacgct ttgaggatct acaactggtg cataacttgc acaagaagaa
     1021 actataatta ttgataaacc aaatattatt tctctcatat aaataaacct cccaaaattt
     1081 tatcgttttt aatttcttcc ttttttggaa accattctag aaaatccgac ttttcaatta
     1141 cggtacgatc tcctctgaaa cttctactac atctccacca gccggttcaa taagtgtaaa
     1201 cccaactagc ggtttataat cacctaattt ataatctctt attgttaata tacatttcgc
     1261 actatttttt tcagtaaagc atgaataaga aaccatgctt cctgcgaggt tcatagaatc
     1321 ttcatcaaca agaaccgaag gaaccaatcc aaaattgtga acgtcaaatc cacctttatc
     1381 atttttagaa actcgaaaca agccattgaa ctctatttct cttgtagtct ttggctctga
     1441 gccagattcc tcaacagtag tttttctaaa ttcctcggaa acaatcgccg tgccccccaa
     1501 attattgata tggtccaaaa cattaatcgt tgtcttttgt tcaagttttc catccttagt
     1561 cttggttatt acaatgctaa ctggtatatc aaattcaccg tcacccacat ctactgcctt
     1621 tccaaattgc ctttcctttt taacaataac accatcctgt gtggtttctg ttacaagtat
     1681 tttgcctgca agtaaactat cataaagctg cctaaattcc ttaacgctaa gtggttcatc
     1741 ttttgcataa acgttcgatg aatacgtggt tattaaagct aaaacaaata ttggtagaat
     1801 aaaaattttt ctcataccta tcctcctgta tcacttcaat atatgtgttt caatttaacc
     1861 tcatctaata tcctaattca agggctactc tatttgtctt tctcaaatct gatgatcgaa
     1921 tttcgtttat ctattttaaa catcaattct taatcaaaag atgagatata ttaacttgat
     1981 aattccctgt agcttgctac agggtttcca caatgttgta ttgtcattgc gaacgaagtg
     2041 aagcaatctc aaagagagat ttaaggagca tatcgatagg ctgggttttc aaaccccgct
     2101 tcctgctgcg ggcaagaatg acccgcctat cgtgtttggt gacacagttc atggataggc
     2161 ggggttttcc aaccccgcat tgggtagaat taaaatatac cgatagacga ggttttctaa
     2221 cctcgtggat taatgctcgt aaatcaatgc gggacaagaa tgtcccgcct atcccctgca
     2281 gttgggtaac atataagcta tcttatggaa aaagaatatt ggtttgtttt aattgccgcc
     2341 atcctatatg gaacgatcac agcaggtggt caattttttg ttaatcaagg tttatctctt
     2401 tttgaaatat ccttatatcg cgttctcttc ataagtctac tactcttacc aatagttttg
     2461 attaagcgtg agttactaat caaaagagga atgctcttat ttttcttggt ttatgggtta
     2521 attggagcgt tcttggaact atcacaattt ggtggaattg ccttaggggt tccagttgca
     2581 attgttgtcc ttctactcta tagccaacct atttggacga ttataattgg aagatttatg
     2641 cttaatgaaa aaatcacact tagaaaggtt ttagcggttt ttatagccct atcaggcgtt
     2701 tttattatac tcaaatcatg ggatatacaa tctccgaaat ctttagcagg agttatttca
     2761 tcacttcttg ggggtatcat tttatctcta tgggtaattt ggggaagaat gagcggaata
     2821 aataaacagc attatgtaac tacaacattc ggatggaccg ctttttctgt gatatggctt
     2881 ttattgcttt ggccaattgt aaatatattt attagagagc caactatcat ccgaatatca
     2941 accgatttcc cattaaggta ctggttctac tttgcaattt ttggatttat tggtggagta
     3001 atcccgcact tattcttcta tagaggtgtt caaaaagttc aagctggagt tgcaggaatt
     3061 atcctactct tggagccagt tagtgcaaca gtacttgcgg atattttatt caatcagcca
     3121 attggcctaa atatatttat tggcggtgcc ttaatattgg tctctaatta tttaataatt
     3181 cacaaatcag aataaaatct ggcttttatt ttcaactcaa gaacactaca gcaagatgta
     3241 gagtatcgtc atatgagcca gataaccctt ttagtcaaaa gtatatcgat aggcggggtt
     3301 ttcgaacccc gcatctggtt gaattaaaat ataccgatag acgaggtttt ctaacctcgt
     3361 aatgtaatgt gagacaagaa tgttcagcct atcgtgtttg gtgacacaat tcaccgatag
     3421 gcggggtttt cgaaccccgc atgccattac tccgcgaatg tgttcgcaat gacatcggaa
     3481 accctagtaa caagctaaac agaattgcct aattaaggcc ttgatgtttt gaaaataatg
     3541 gttaatatat aaggaagttt aaaaaaatta gaaatagggc aagaaatacc cggaagaggt
     3601 acttgccaag aggataaaag ataagatgaa gagtatatat aaggataatt ttgtttactt
     3661 aaagaacctt acgcaattga tctacgaaac cctgggtgaa tatcaacacc tctggtttaa
     3721 tctaataaaa ccagtaaatc ctcctttaga aagagaatat gaagatgatg aggaattacc
     3781 agggatccag aaaaagggtc acctatttct ttcagaatta tatcgcttat gcaaagaaga
     3841 ccaatccaag gagtttaata tgtatgagat tggtgaaaga ctgggatttg atgaatttga
     3901 aacagaactt atagtagaaa atctcgcaaa ttcagaattg attagtcatc aaaaaagctc
     3961 cgacgaggtt tcgattacgc cttatggaat tatgacgata aagggcgaaa tacgtattgg
     4021 atatgccccg gttcattaaa acaattcact cagaaagtcc aaagactagg ttttagactg
     4081 ctctagctac ccaggcgagc ataccctacc tcagttggaa tgcttgccct ctcaatctta
     4141 gccttagaaa aagcctgaat tctttttagc tcgccatatt cccagtgagt tacaaatgtg
     4201 atagcctttc cctcttttcc gcatctacct gttctaccaa ttctgtggac ataactttca
     4261 gcgtcctgag gaatatcata attcacaaca tgagtaatac catctatatc gagccccctt
     4321 gccgcaacat ccgtggcaac tacaatgtcg acctcacccc ttctaaagag ccccataacc
     4381 ctatcgcgct ccctttgaga gcaatcccca tgtagcgcct gggcattata acccctggct
     4441 ttaagggcag ccgtcaaatc atcaaccccg gcttttgtcc ttcgaaagac aaggaatacc
     4501 cctcccctct ctaactcgat taatttactt agaacaccaa gcttacccat ctccctgacc
     4561 tcaaaaaata tctgcttggt ttctggaact gtaagggtat ctttggagac cattatctcg
     4621 acagggtctt tcatatatcg ctttgcaagc caggagatct ccgatggtat agtcgcagaa
     4681 aatagcatga tctgacacct tccaggaatt tgcgcaagaa taaacttcat atcatcaata
     4741 aatcccatat caagcatcct gtccgcctca tcgaggacag caatacgaac ggattttagg
     4801 ctcagtgtcc cccgccttat atggtcaatg acccttcccg gtgtaccaac aacgatgttt
     4861 acccccttac taagctgatc caactgcttc ccaataggct gaccgccgta aactggaagc
     4921 actgttattc gcttatgctt cccgattttc ccaagttcct tagacacctg aaccgcaagt
     4981 tctcttgttg gagtaagaac tattgcctgg acttccttgt ctaaagggtc aatcctttcc
     5041 actataggaa ttccgaaggc aacggttttc cccgttcccg tctgagcctg gcctattatg
     5101 tttctgccac ttaaggcatg tgggattgct tcctcctgaa tcggagtcgg aatactaaat
     5161 cccatatccc ttatagctct gttaacactg tttgataaaa taaactcttc aaacttcaat
     5221 ttacaaacct cctcgttagt ccaaaattgg acctttgatt ttatttttga gtcttttcag
     5281 ttattcgtcc taggagggta aaaagaagtt tttctactaa tttggagagt aagtgaatta
     5341 ctctagaaat gaggaaacta tttaccttcc aagctaaact caattagact ataggtaaca
     5401 atgttacatg caaaactaaa attatgcaaa tttcaatata atttgtcaga gtaaataaac
     5461 ctccttacat ctctattctt cttgaatcct gtcagagatg acaaactttt attacaacta
     5521 cttgaagaat aatctcttgc cagcatacct aaataagaca atggggtgaa tgggacaaaa
     5581 gggttttatg taggacatga tagggcgata tatacaaggg ggataaagca gattcgggct
     5641 cagagtccaa acctttggac atggatagct aaaggggggc gaatgggaca agtaagagac
     5701 aggttaaatc aaatagtgct ccatatctgc gatggtttga taatattctc gatagacatt
     5761 caaagttaat ttcgactttg aagctgaagt gggctagatt tattagggat agctcaaagc
     5821 aatcacgcta tccctaccat tgcctaaatg gtatggacga gtctaaaaaa tggttggtta
     5881 tatggttata agccgagatt tggagactca tatttctcaa gtcatgattt ctggaactgc
     5941 ttcataaagt agatcaacga aaggatttca aatcttatta tcgcttatat tactaattca
     6001 ctccacttct aatcctgcag cggagttatc tatacctcaa cttttcaaga ataaagatgt
     6061 gttcgcattg gagagacgca tagacgccct gaatcaataa agtttgtaac taacaccgta
     6121 gcggaatatc ttaagggtgg gaaatgtcta atagaatatc taatagtggg tttagaaatc
     6181 ccatcacacc aacagccagt tttagagaga gctttcttgg gggtatggag accaaacaga
     6241 atctccattt cctcctctac ctcgtgttga tccaggttgc cctattccga aggcataaac
     6301 ttaggtgaaa ccttagtcgg aacccacagt taatagagta tagaccctaa gactgggttg
     6361 acacaatatg ccatgtccta taatctacgg gaaaaggggt tatggttgga ggtgttaaat
     6421 gaaaacagtt aaattccttt tagtggcaac gcttattacg ctatcacttg caaccgtagg
     6481 gtttggtcag caagcaggtt aaggtcggca tggaggaggc ggtggtcaag gtggttggca
     6541 tggaggacga gggggtcatg gcggcggttg gcacggtgga catggtggtg gatggtgagg
     6601 cggtcatggt ggtggatggt atggtggtcg tggtggttgg catgggtact acccaaatca
     6661 gttttacttc aacttaggga ttccgttcat ttccccctat tactacccat atggaaacta
     6721 ccaaccttgt tatcctcctt accaatatta tcctcagtac tatccgtatg gagcgtaccc
     6781 ttacatgaac ttcggattcc agctctacgg tcgttaatgt agcagaactt ctgagtgaca
     6841 aatcggcata gtgcctagag gcagaacagc acagaactct gtttactgga agaaatagtg
     6901 gagaaatagg ggtcaggtct ttaatcgtgc attcatactt tattatcaac acgcccgtta
     6961 aaaaacttat atttcgagac ctgacccagg ttggttggtg agcttgtgtt cgcttcaagt
     7021 gaagttagct ctgatagctt tttattaagt tccttttctt ctggtgtttg ccttcgtgtg
     7081 attttagttc ccaatgctga tagtgtcctt tgcttacgct ttactaacct gatccctttt
     7141 ggatttaata ttgaatagta ccatgtgctt tttgttcaag gcccgcctcg taggatttct
     7201 tataaaatag atttggctat cttgaatcct atccaaatac caataaaacc accaatcaca
     7261 cttaatatca aggatgataa cgaaaaaaag ctcgctcccc atagtttcgg tataaaaccc
     7321 ccaattgtag aacctacaaa caaaccaatc catattaaag attttgtatt catagtatta
     7381 ttaatatatt ttatccgtaa actttattct atttccatta tcccaccaag gatattccct
     7441 tgtcaacacg cccaaatatg gcaaaagtag gaataggaga gcatcatagt tattgcttt
//
LOCUS       DATDRL010000025        10880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3689094, whole genome shotgun sequence.
ACCESSION   DATDRL010000025 DATDRL010000000
VERSION     DATDRL010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10880
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3689094"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VI935_01015"
     CDS             complement(<1..1322)
                     /locus_tag="VI935_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLE24211.1"
                     /translation="MKDPLEEIINIVGKEQSVSLHKELEHYSVDGKRPNLVVFPETIN
                     EISEIMKVASSESVAITPWGGGTKIGLGRVPDKVDLVVCTGRINRILEHEASDLIATA
                     ECGIKLKELQRVLREKNQFLAIDPPHVELGATVGGIIATNDSGPKRLKFGTMRESLIG
                     IKVVRPDGSIVKGGAKVVKNVAGYDLPKLYVGSLGTLGIIVEGTFRLYPIPEASKTYL
                     ATFPNLEMFQETVLSILNTSIVPTCLEALNPDLIGAISNKLNLDLRKQKYALAVRIES
                     LEKAASDQISKVKQISDEKNGEGLQLEGDIEENLWQEIREFPWRISCENRTVCKASIL
                     ITDVPGVFQASEELCKKSGLKTYISGRAGNGVLIFGIEVKRPFDCAQGSPQEEMLAVV
                     DAIKSLRALVSSLKGTLVIQDAPLSIKSQVDVWGEIGASHKVMEKLKNL"
     gene            complement(1496..2983)
                     /gene="glcD"
                     /locus_tag="VI935_01020"
     CDS             complement(1496..2983)
                     /gene="glcD"
                     /locus_tag="VI935_01020"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015159955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HLE24212.1"
                     /translation="MASKTITKTSNWEKSFADKLLQILGTDGVISSPDELIAYECDGL
                     TGYRVRPICVVLPKSAKEVSEVIKLCNIENIPFVPRGAGTGLSGGALPVEEGLVISLS
                     RMNRILEIDIPNQRVVVEPGVINLWVTDAVNSSGYYYAPDPSSQLVCTIGGNVAENSG
                     GVHCLKYGVTTNHVLGLEVVLPDGEIVEVGGKTLDYPGYDLLGLFVGSEGLLGIVTKV
                     TLRILRKPESIRTLLAAFEKVEDAGASVSVITARGIVPAGMEIMDNLAIRAVEEAVQA
                     GYPVDAGAILLVELDGPSAEVEAQMPLVIGVLKENRAKEVRIAKDDDERALFWKGRKS
                     AFAAMGRISPDYYVQDGVIPRTKLAEVLSEIGKLSTKYRLRVANVFHAGDGNLHPLIL
                     YDSSKAGELEKAEELAGEILKVCVDAGGSITGEHGVGYDKKQFMSLMFSEDDLDTMYL
                     IRCAFDSKGLCNPGKVFPTPRSCVEAGRTSYREHPIEKSGLGERF"
     gene            3542..4300
                     /locus_tag="VI935_01025"
     CDS             3542..4300
                     /locus_tag="VI935_01025"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HLE24213.1"
                     /translation="MKYIKFTVIALCLIVLILPTLSAKQLYKWVDKNGVVHVTDNPDS
                     IPSEYRSSAVKSKPKSRSGATIEKIENGFKNVFDEVSGLVKENIKKVVAGALVIVGLI
                     ALVYLFRGYLKFKEQKERERGLRALEILNIDGISRTEFENYVIRLLTHRGFKVETTGE
                     KIALGVTLIAKKDDHKYAVQIERQTGSVSRLAVSDIDREKHRYGCDKVMLITNGYFSE
                     DAIELAKSTGCELVDRDTLAKWVLDFQRSNVESS"
     gene            4396..5520
                     /locus_tag="VI935_01030"
     CDS             4396..5520
                     /locus_tag="VI935_01030"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLE24214.1"
                     /translation="MSFVDAHTHLEFFALKGLPVEKAKTKKDLVDMIESAERKPVVAY
                     GWNEEILGETIERKDIDRFSFPVLLIRVDAHIGVINKKVMEELQLNPSEKFDPERGYV
                     YEEVLWEITSMLKPKEIGWALIKGQEEALSKKIVEIHDFVDSKIAEIYFRLREEGNLK
                     LRVALMPFYKDYEKVLRLFDKYGEDDFLKLGWIKIFVDGSIGARTAYLREPYRDKTSK
                     GILLQTEEELINIIKELEAKNLRVAIHAIGDGAIDVCLNTFERADIRFKGHRIEHAEM
                     IDSYQAKRVNDMGITLCVQPNFNPVFMQTYIKALGEDRAHRMNPLKMLDEIGVNMIFG
                     SDMMPFDPGVGSDYASQILGKEKALYYYGGWKHNRTYNYL"
     gene            5697..6317
                     /locus_tag="VI935_01035"
     CDS             5697..6317
                     /locus_tag="VI935_01035"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HLE24215.1"
                     /translation="MTSVIKEENPDSPKIVASVLNNGGIIIYPTETLYGLGALAVNEE
                     SAKKVFDIKGRLQANPIPILVRDKEMLSEYVVVTDEANRLIEKFLPGPLTLVLKEKKN
                     LPYLISAGTGTIAIRISGHPFVKRLFDFLFQPLTSTSANISGQENLFSFDEIYKTFNS
                     KVELIVDSGNLPRSKGSTVIDLTVKPALIIRKGDISSVRLKEFINL"
     gene            6471..7697
                     /locus_tag="VI935_01040"
     CDS             6471..7697
                     /locus_tag="VI935_01040"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HLE24216.1"
                     /translation="MVMVVPFCGVRYNADKVGVLDSVFAPPYDVISPQEQNKLYEKNP
                     YNVIRLILSKGEGDEKYIEAAETFRRWIDEGVLIQDREPCIYPYYQEFKEEGKVVTRK
                     GFIAAARLEDFSSKKIIPHERTFAAPKLDRLKLTTACRANLSPVFAIYSDPEGALEKS
                     IEDRLSKEPIVRAVSGDGVKNNLWRISDSDLLSQIKSMMLDRVLLIADGHHRYETALE
                     YRNIQRKMADESPEDKDYEYVMMYLSRVEGEGLVIKPTHRVVKNLGPFDTEDFLKKLR
                     NKVYVEKVSFNEGISTLDHQEFAIAIKDSDFIHKVSSKEPLSFGYKNLGVMLLHEEVF
                     GKIINQEEAEILYTKSVEEVVRLVRTRDYKIGFILPQVSAKDLYEVALSGEKMPHKTT
                     YFYPKLLSGLVFHLLD"
     gene            7721..8278
                     /locus_tag="VI935_01045"
     CDS             7721..8278
                     /locus_tag="VI935_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HLE24217.1"
                     /translation="MLAFLLIVIILGILVAAFVGIYNSLVKLRNTSEQAWSDIDVQLK
                     RRYDLVPNLVETVKGYAAHEKSTFEKVVQARNAAMQASSPIDKAQAENILQSTLRSLF
                     ALAESYPELKANQNFIELQDELSKIEEQIQLSRRYYNAVVRDLNTKIETVPSNVIANM
                     FAFQKKEYFELESAEERRVPEVKFG"
     gene            8543..10246
                     /locus_tag="VI935_01050"
     CDS             8543..10246
                     /locus_tag="VI935_01050"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HLE24218.1"
                     /translation="MKSIIIVTFSLLISSLSFPVYSEEIRDFYSEILISSDGSINVQE
                     NIEYDFGYELRHGIFREIPYKYEIGIKNYNLRMNVIRVTDFEENLYSYKVSRERGRVN
                     VRIGDPDKEITGVHEYRIEYFIDGAILFFKEHDELYWNVTGNEWKIPILTASAKIYFD
                     KEILEGVKATCYTGIYGSKSQNCTSNILPNGIEFDVLGSLRAGEGLTIVVGLPKGILK
                     EPSSLKKALWFISDNWFFALPILTLFFISYVWYTRGRDPEGKGVIAVRYEPPKDLTPA
                     EAGTLMDERADILDITSTVIDLAVRGFLRIQEVLGTKFIFFSDRDYNLIKLKDLSGDT
                     LKAHETTIFTSIFEGDKKEVIVSDLRNKFYKHLPPIKNALYNELISGRYFPTNPENIR
                     RILKWIGIGIVVVSFVLIPHWDIKLSLIISGIFILIFSRFMPRKTKKGSLAKEEILGF
                     REFIERAEKDRIERLAKEDPTLFDRVLPYALVFGLGDRWAEAFHEMYKSPPSWYDSPR
                     YGDSFSPRIFVNDLGRSLSVMSSTFASTPSKSGGYSGGSGFGGGGSSGGGFGGGGGGS
                     W"
     gene            complement(10368..>10880)
                     /locus_tag="VI935_01055"
     CDS             complement(10368..>10880)
                     /locus_tag="VI935_01055"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HLE24219.1"
                     /translation="WKPKEGLELIGTKAPSFEGLNWLNTEPLNIEDLKGKVILIRFWL
                     VGCPLCEHTAPALVELYNKYKNDGFIVIGIHHPKSEEAKDPNLVRRALDAFDFDFPVA
                     QDSDWKVINAYWLGGKKRSFTSSSILIDKNSIIRFVHDGGEFYKSENNPDADLAYQAI
                     EEKIQELLGE"
BASE COUNT         3332 a         2051 c         2265 g         3232 t
ORIGIN      
        1 aggtttttta acttttccat taccttatgc gatgctccta tctcacccca tacatctact
       61 tgggatttta tagataaagg ggcgtcttga ataaccaggg taccttttag agaactaacc
      121 agagcacgaa gggatttgat ggcatcaacc accgctaaca tttcttcctg tggcgagccc
      181 tgagcacagt cgaagggtcg cttcacttca attccaaaaa ttaaaactcc gttacctgct
      241 cgtcctgata tataggtctt gagtccagat tttttacaca actcctctga tgcttggaaa
      301 acccctggga catctgtaat aaggatgctt gccttacaaa ccgttctatt ttcacaagaa
      361 atgcgccatg gaaattctct tatttcttgc cagaggtttt cttcaatgtc tccttcaagc
      421 tgaagacctt ctccattttt ttcatccgaa atctgcttta ctttagaaat ttggtcgctt
      481 gcggcttttt ctaggctttc tattcgaaca gcaagtgcat atttctgctt tctcaaatca
      541 agatttaatt tgttagagat ggcacctata agatcgggat tcagagcctc caagcaggtg
      601 ggcacaatag aggtgtttaa aattgaaaga acggtttcct gaaacatctc gagatttgga
      661 aaggtggcta agtaagtttt ggaggcttcc ggaattggat agaggcgaaa agtaccttca
      721 acaatgattc caagggtacc aagggaacca acatagagct ttggaagatc atagccagca
      781 acgtttttaa ctacttttgc cccgcctttt acaatacttc catctggtcg gactacttta
      841 attccgatta gggactctct catcgtacca aacttaagcc ttttcgggcc actatcattt
      901 gtggcgatga tcccaccgac ggttgctcca agttctacat gaggtgggtc gatagcaagg
      961 aactggtttt tctctctaag aactctttgc aactctttca gtttaattcc acactctgcg
     1021 gttgctataa gatcagaagc ctcatgttca agtattctat taatacgacc tgtgcatact
     1081 acaaggtcaa ctttatctgg aactctgcca agaccgattt ttgttccacc tccccatgga
     1141 gttatagcaa ctgattcaga tgaggctact ttcattatct ctgagatttc atttattgtt
     1201 tctggaaaaa caacaagatt tggccttttt ccgtctaccg agtagtgttc aagttcctta
     1261 tgtaaagaga ctgattgctc tttaccaact atattgatta tttcttctag tgggtctttc
     1321 atggtttttt tcgtaggagc atgtttatgt ttttgacaat ttaatcagct aataaacatg
     1381 agggcagccc accagtcttg ctggtgggct agggggcaac gagcaagact cgttgtctgc
     1441 ccagttggtt atctattact tgtttaataa ctcaagggcc gtcaatgcgc ctcatttaaa
     1501 atctctctcc taaaccagat ttttcaatcg gatgctccct gtatgatgtt ctccccgctt
     1561 ccacgcagga tcgcggcgta gggaatacct ttccaggatt gcataatcct tttgaatcaa
     1621 atgcgcatcg gattagatac atggtgtcta agtcgtcttc actaaacata agggacataa
     1681 attgtttttt atcatatccc acaccatgct ctcctgtaat gcttccacca gcatcaacac
     1741 agaccttgag aatttcaccc gccaactctt ccgccttctc taactctcct gcttttgaag
     1801 aatcataaag aattagagga tgaagattcc catcgcctgc gtgaaaaaca tttgctaccc
     1861 gtagcctgta tttagtgctt agtttcccaa tctcactcag tacctcagca agtttagtcc
     1921 ttggaataac accatcttgg acataataat cagggctaat ccgtcccatc gctgcgaagg
     1981 cactttttct tcctttccaa aatagcgctc tttcgtcatc gtcttttgca atcctcactt
     2041 cctttgctct gttttccttt aataccccta taacaagtgg catctgagct tcaacttcag
     2101 cacttggtcc atcaagttca actaaaagta tagctcctgc atctactggg tatcccgctt
     2161 ggacggcttc ttctactgca cggatggcca aattatccat aatctccatt cctgctggaa
     2221 cgattcccct tgcggtaatc acggataccg atgcaccggc atcctcaacc ttttcgaagg
     2281 ctgcaagaag tgttcttata gattcaggtt ttctaagaat tcttaatgtc actttggtaa
     2341 cgatccccaa aagtccctct gaaccaacaa acagtcctaa aagatcatag cctggatagt
     2401 ctagtgtttt tccgccaact tccacaatct ctccatcggg gagaaccact tcaagaccga
     2461 gaacatgatt agtcgtaact ccgtatttta gacaatgaac tccgccagag ttttctgcga
     2521 catttccacc tatggtgcag accaattggc tagaagggtc tggtgcataa tagtatccac
     2581 tgctatttac agcatctgtg acccagaggt ttattacacc tggctcaaca accacccgtt
     2641 gattgggaat gtcaatctcc aaaattcgat tcatgcgcga taaggaaatt acaagcccct
     2701 cttccacagg tagtgcacca ccagaaagtc cggttccagc acccctaggc acaaagggaa
     2761 tattttcaat attacaaagc ttaataacct ctgaaacctc ttttgccgat ttgggcagaa
     2821 ctacacatat aggtctaact ctgtatcctg tgagcccatc gcattcatat gctataagct
     2881 catcaggtga ggagataact ccatcagtgc ctaggatttg aagaagttta tcggcaaaag
     2941 atttttccca gtttgatgtc ttagttatag ttttgctggc cattttagtt tcttcgtgac
     3001 cctctacata gagcattata tcactgatga aagtgttaac tcaaatggaa tatattttga
     3061 agttgtaaat agacaagagg tgaaactaga attatgtgta taagtaattt gtagtttaac
     3121 ttgagaattc gatgtacctt gctacagact tccactgtta tcatcgcgtt attgtaaata
     3181 ttctttttct tgtcatttcg aaccatgtcc tgagcgggag cgaaggaaca gcgagaaatc
     3241 ttagattcct cactacgttc ggaatgacac attgcgtcag attgcttcgt ccccttcgac
     3301 ttcgctcagg gtcctcgcaa tgacagctat aacggtatat ttttaagact ataccatgca
     3361 acttgcaggc tgtccgacaa ttatggatgt tgaagatctg actcctcaga ataccccagg
     3421 atcaacgatc ggtaatctgg gaggggtttt gctacccccc aagaatgcgg atttttgcat
     3481 ttattggata gcctacttgc tgcacggtta ttaatttgta tttataataa acacttaaaa
     3541 aatgaaatac ataaaattca ccgtaatcgc tttatgttta attgttttaa tactacctac
     3601 tttatcagct aaacagctat acaaatgggt tgataaaaat ggtgtagttc atgttactga
     3661 taacccagat tctataccta gcgagtatcg gagctcggca gttaaatcga aacccaaaag
     3721 cagaagcggt gcaaccatag aaaaaataga gaacggtttt aagaatgttt ttgatgaggt
     3781 ttcaggactc gtaaaggaaa atattaagaa agttgtagct ggagccctcg taattgttgg
     3841 attaatcgca ttagtctatt tattccgcgg ttatctcaaa ttcaaagagc aaaaagagag
     3901 agaaagagga cttcgagcct tagaaatatt aaatatagat ggtataagtc gaactgaatt
     3961 tgaaaattat gttattaggc ttttaactca ccggggattt aaagttgaga cgacgggaga
     4021 aaagatagct ctcggtgtaa cattaattgc aaagaaggac gaccataaat atgctgttca
     4081 aatagaaaga caaaccggct cagtttcgag acttgcagta agcgatatag atagggagaa
     4141 acatcgctac ggttgcgata aggtaatgct gattaccaat ggttatttct ccgaagatgc
     4201 aattgagtta gccaaatcaa caggatgtga actggttgac agagataccc ttgctaagtg
     4261 ggttcttgac tttcaaagat cgaacgtaga atcttcataa cgagcattta aaattcattt
     4321 tattctcctt ggtactttta gcagttcact ttataggagg agaaaaattt ctttcaagta
     4381 atcgatattg aagtcatgag ttttgttgac gcgcatactc acttggagtt ttttgctctt
     4441 aaagggttgc ctgtagaaaa ggcaaagacc aaaaaagatt tggtggatat gatagaaagc
     4501 gcggaaagaa agccagtcgt agcatacggt tggaatgaag aaatactcgg agagacgata
     4561 gaaagaaagg acattgatag gttttccttt cctgttcttc ttataagagt tgacgcacat
     4621 ataggagtga taaataagaa ggtcatggaa gaactacagt taaatccttc tgagaaattc
     4681 gatccagaaa ggggttatgt ttatgaggag gtgttgtggg aaataacatc catgttaaag
     4741 ccaaaggaga taggttgggc tttaattaag gggcaagaag aagcgttatc aaagaagata
     4801 gttgaaattc atgattttgt agatagtaaa attgcggaga tatacttcag acttcgagag
     4861 gaaggcaatc taaaattgag agtagcttta atgcccttct ataaagatta cgaaaaggta
     4921 ttgaggcttt ttgataaata tggtgaagat gattttctaa aactcggatg gataaaaata
     4981 tttgttgatg gttcaatagg tgcaagaact gcgtatctca gagagcccta tagggacaaa
     5041 acttctaaag gaatcctttt acaaactgag gaggaattaa tcaatatcat aaaagaactc
     5101 gaggctaaaa atctacgagt cgcaattcat gccataggtg atggagctat tgatgtttgt
     5161 cttaatactt ttgaaagggc tgacataaga tttaagggac acagaataga acacgctgag
     5221 atgatagatt catatcaagc aaagagggta aatgatatgg gtatcaccct ttgtgttcaa
     5281 cctaatttta accctgtatt tatgcagacg tatatcaaag ctcttggaga ggatagagcg
     5341 cataggatga atcctttaaa gatgctcgat gagataggcg ttaatatgat ctttggatca
     5401 gacatgatgc cctttgatcc gggggttggg tcagattacg cttcccaaat tctaggtaag
     5461 gagaaagcgc tatactatta tggtgggtgg aagcataata gaacttataa ttatttgtaa
     5521 atgagatatt tcaaataact cttcacggag agtaggatag ttacgcatag ggatattgtt
     5581 ttgaagagct agaatagacg ttgaatagat tatttcagtt ctgtaaatta aaaaagaaag
     5641 acctaccaat tatgcacctt agacaaattc gataaataaa atattggcat ttaaccttga
     5701 catcggtaat taaagaagaa aatcctgact cacctaaaat agtggcgtcg gttctaaata
     5761 atggcgggat tattatttat ccaacggaaa cactatatgg tttaggtgca cttgcagtca
     5821 atgaagagtc agcaaaaaaa gtttttgata taaagggaag gcttcaagca aatccaattc
     5881 caatcttagt aagagacaaa gaaatgctct ctgaatacgt tgtggttaca gatgaagcca
     5941 ataggctaat cgaaaaattc cttccaggtc cactcaccct agtacttaaa gaaaagaaga
     6001 atctgccata tcttatttca gcgggtactg gaactatcgc tataagaata tcagggcacc
     6061 cgttcgtaaa gcgattgttt gatttcttat tccaacctct aacctcaaca agcgcaaata
     6121 taagtggtca ggagaaccta tttagctttg atgaaattta taaaacgttt aatagtaagg
     6181 ttgaacttat agttgattct ggtaaccttc cccgatcaaa aggatctaca gtaatagact
     6241 tgactgtaaa accagctttg ataattagaa aaggggacat tagcagtgtg agattaaagg
     6301 agtttataaa tctttagcca caacaaacta actctttctg tcattccgag cgaagcgagg
     6361 aatctcaatg agattgcttc gtcacttagc tcctcgcaat gacattcaag tgttgtcgtt
     6421 ccgagtaaat tcgtgtttat tagtggcaga tgtttattgg agttttttta atggtaatgg
     6481 ttgttccttt ttgtggagtt cggtataacg ctgataaagt aggggttctt gattctgtgt
     6541 ttgctcctcc ctatgacgtt attagtcctc aagaacaaaa caagctttat gagaaaaacc
     6601 cttacaacgt aatacgtctt atactttcaa aaggtgaagg cgatgaaaaa tacatcgagg
     6661 ctgcggaaac ctttagaaga tggattgatg agggggtgct cattcaggat agagaaccct
     6721 gcatctatcc ttattatcaa gaatttaaag aagaagggaa ggtagttacg cggaaaggat
     6781 ttatagcagc agcaagactt gaggattttt catcaaagaa gattattcct catgaaagaa
     6841 cgttcgctgc accaaaattg gatagactca aacttactac tgcctgtagg gcaaacttaa
     6901 gtccagtatt tgcgatctac tctgatcccg aaggagcgct ggaaaaatct atagaggata
     6961 gactatctaa ggaacctata gttagagcag ttagtgggga tggagtgaaa aataatctct
     7021 ggagaatatc ggacagcgat ttactttccc aaattaaaag catgatgcta gatagagtgc
     7081 ttctaatagc agatggacac caccggtatg agactgcttt ggaatataga aacattcaaa
     7141 gaaaaatggc tgatgagagt ccagaagata aagattatga atacgtcatg atgtatctct
     7201 cgcgtgtgga aggcgaaggg ctagtgataa agcctactca ccgtgtggtg aaaaatctcg
     7261 gaccatttga tacagaagat tttcttaaga aattaagaaa caaagtttat gtagagaaag
     7321 tgtcctttaa tgaaggcatt tccacgcttg atcaccaaga atttgctata gctataaagg
     7381 attcagactt catccacaag gtttcctcaa aggagccatt atcatttggt tataagaatc
     7441 ttggagtgat gcttcttcac gaagaggtgt ttggtaagat tataaaccaa gaagaagctg
     7501 aaatactcta cacaaagtca gtagaagagg ttgtcagact tgtaagaact agagattata
     7561 aaattggatt tatacttccc caagttagtg caaaagactt atatgaagtt gccctttcag
     7621 gagaaaagat gcctcacaag acgacttatt tctatccaaa gctcctttca gggctagtat
     7681 ttcatttgct tgattaaaga aatattagga ggtagagaaa atgctggcat tcttacttat
     7741 agtcataatt ttaggaatcc tagttgctgc ctttgtaggt atatacaatt cccttgtaaa
     7801 actaagaaac acatctgaac aggcgtggtc agatattgat gtgcagctaa agagaagata
     7861 cgatcttgtt cctaatctcg ttgaaacagt aaagggctat gcggcacatg agaaaagcac
     7921 gtttgagaag gtagttcaag cgagaaatgc cgccatgcag gcttcttctc caatagacaa
     7981 ggcacaggct gaaaatatcc tacagtcaac tctaagaagc ctttttgcac ttgccgagag
     8041 ctatcccgag cttaaggcaa accagaattt tatagagctt caagatgagc tatcaaaaat
     8101 tgaagaacag attcaactct ctcgccgcta ctataacgct gttgtaagag acctgaatac
     8161 taagattgaa accgttccaa gtaacgtcat tgcaaatatg tttgcctttc aaaagaagga
     8221 atattttgag ctggagtctg ctgaagagag gagggtgcct gaggtgaagt ttgggtaata
     8281 gcaacttgtt atatagcttg cattctgaaa cgggtatgaa tcataaaatg tcattgcgag
     8341 gagtgaaacg acgaagcaat ccgattggga ttcctcactc cattcggaat gacacttcgt
     8401 gtcagattgc ttcacgaagt ttacactgag tgaaacgaat gtgttcacaa tgacaaatgt
     8461 aggagattgc ttcgttagca ctcgcaatga caaattcaat tgacgccaca attatatcgg
     8521 aaacagtttt aatcagattt taatgaagtc tataattatt gtcacatttt ccttattgat
     8581 ttcctccctc tccttccccg tttactccga agagattaga gatttctaca gtgagatact
     8641 tattagtagt gacggttcta tcaatgtcca ggagaacatt gaatatgatt ttggatatga
     8701 attaaggcat gggattttta gagagattcc atataagtac gagataggta tcaagaacta
     8761 taacctcaga atgaacgtaa tcagggttac ggattttgag gaaaatctat acagctataa
     8821 agtcagtcgt gaaaggggaa gggtaaatgt tagaatcgga gatccagata aagaaataac
     8881 tggagttcac gaatatagaa tagagtactt tatagatgga gccatacttt tctttaaaga
     8941 acatgatgaa ctctattgga atgtaacagg taatgaatgg aaaatcccaa tattaacagc
     9001 aagtgctaag atttacttcg ataaggaaat cctcgaaggt gtaaaagcaa cttgttatac
     9061 aggtatatac ggttcaaaaa gtcagaattg cacatccaat attttgccaa acggaattga
     9121 gtttgatgta ttaggaagct tgagagccgg agagggcctc acgatcgttg taggattgcc
     9181 taaagggatt ctcaaggaac cctcttcgct taaaaaagcc ttatggttta tctcggataa
     9241 ctggtttttt gctctcccta tcttaaccct attttttata tcatacgttt ggtatacaag
     9301 aggcagagac ccggagggta aaggagttat tgctgtaagg tatgaaccgc ccaaggattt
     9361 aacccctgcc gaagctggca cgcttatgga tgaacgtgct gatattcttg atattacatc
     9421 aacagttata gaccttgctg taagagggtt tcttaggatt caggaggttt taggtacaaa
     9481 attcattttc ttctcagata gagactacaa tctgataaaa cttaaggatc tctcaggaga
     9541 cacattaaaa gctcacgaaa ccactatctt taccagtatc tttgagggtg acaaaaaaga
     9601 ggtaattgtc tctgaccttc gaaacaaatt ttataagcac ttaccaccta taaaaaatgc
     9661 actatacaac gagcttataa gtggtagata ctttcccaca aatccagaga acataagaag
     9721 gattttaaaa tggattggga taggcatagt agtcgtatcg tttgttctaa ttccacattg
     9781 ggacataaag ctttcgctta ttatatccgg gatttttatt ctcatattct caagatttat
     9841 gcctagaaaa acgaagaagg gttctttagc aaaagaagaa atcttgggct ttagagagtt
     9901 tatagagcgt gcggaaaaag atcgaattga aaggcttgcc aaggaagacc caaccctttt
     9961 tgatagggtg ctaccatatg ctttggtatt cgggctaggt gatagatggg cagaggcttt
    10021 tcatgaaatg tataagagcc cgccctcctg gtatgattct ccaagatatg gagattcatt
    10081 ctcacctcgt attttcgtaa acgaccttgg aagaagcctc agtgttatga gcagtacctt
    10141 tgcatccact cccagtaaat ctggtggata ctctggaggt agcggatttg gaggcggtgg
    10201 atctagcggt ggaggatttg gcggaggcgg tggagggtcg tggtagttta tattctctca
    10261 aaaggcatta atttttgctt ggtacgttca gggaacgtac cctacaatgt ttgatattgc
    10321 cacaccaaat gtagcgcccg ttcccagaac ggacattaat tttttgctta ttctcccaat
    10381 agttcttgga ttttttcttc gatagcttgg taggcaagat cggcatcggg gttattttcg
    10441 cttttataaa actcgcctcc gtcatgaaca aatcggatta tgctgttttt atctataaga
    10501 atgctggagg aagtaaatga gcgttttttg ccacctagcc agtaggcgtt aataaccttc
    10561 caatcactat cctgtgctac aggaaaatca aagtcgaatg catctaatgc gcggcggact
    10621 aaatttgggt ctttagcctc ctctgactta ggatgatgaa tccctattac tataaaacca
    10681 tcatttttat atttgttgta aagctcgact agagcgggtg cagtgtgttc acacaatgga
    10741 catccgacta accagaaacg aatgaggatg actttacctt ttaaatcttc gatatttaaa
    10801 ggttctgtat ttagccaatt cagtccctca aatgagggtg ctttggttcc aattagttct
    10861 aggccctctt taggcttcca
//
LOCUS       DATDRL010000026         6636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3115811, whole genome shotgun sequence.
ACCESSION   DATDRL010000026 DATDRL010000000
VERSION     DATDRL010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3115811"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..142
                     /locus_tag="VI935_01060"
     CDS             8..142
                     /locus_tag="VI935_01060"
                     /inference="COORDINATES: protein motif:HMM:NF044424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24220.1"
                     /translation="MHPSFGQGVVKRVEGLGDEAKVVISFHDHGEKKIMASFLGLKKI
                     "
     gene            644..2311
                     /locus_tag="VI935_01065"
     CDS             644..2311
                     /locus_tag="VI935_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLE24221.1"
                     /translation="MNGTLTSNWNSRSGTYDVVVIGSGYGGAITACRIAQAKLKPKPN
                     ICILERGMEWIPGMFPDEPEDITQHSLTPLNPLGLYEYRLFKDIDVIQGSGLGGTSLV
                     NANVAIIPEPAVFEQNPWPVGITGNMLAPYYERAAKTLDVGQHPRGMDLLKVRALKKR
                     ADLSPNAKFDLLRIAVNFKVDGIDPETGVERHPCIDCGDCVTGCNVRAKNTLYMNYLP
                     LARKAGVEIFTQVKVLFIKADPNGGYQIHYERFTPRHNLPETGIIKAKRAVIVSAGSL
                     GSTEIMLRSKNQGLKLSDTVCTRFSGNGDFFGISYNGNQRTDILGFGNHPNDPPRSNV
                     KAGPTIVGVIRYDRNKPLKEQITVEDLSVPRGGVIPTRKVLSTLSAGGRDTDPGDLVQ
                     ELARIARDIQFDAGGALNHSMIYLVMGHDDAGGTMNLKNDRLQIDWPGVGDQDVFGRI
                     NSELFQHAKALGATFIESPVWSFFHSRRLITAHPLGGCPMGETSHLGSVDECGRVYNG
                     KSDEEIHPGLYIADGSVIPTSIGVNPFLTISALSEYIAECIVKELKK"
     gene            2363..3952
                     /locus_tag="VI935_01070"
     CDS             2363..3952
                     /locus_tag="VI935_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24222.1"
                     /translation="MNVNHLEVIGYREDQLKKIFESSETLSMDIMINSGKRKIDRRNR
                     IIYNDVYWKGFFPVGHVLNLFSSNFHTGFKKRLFKEAGHIAGVTSDSDGFINARNLLE
                     VKKDSQLGKHILLRYIESPWQGFYDIFKVLSDKLLIGRVFLGPYPHGTEMFTFCMVRE
                     YTFDDITVDDHRELFDTAKIPKLEEIEGVWDMAAVSNSNHRAGVARLEFDVKPDHRIE
                     GRYQFMSLVQGQMRTELTEDELRMHDFTPLHDEIRSIDVDYMIGKWCSARKETFGPLS
                     AGLLQFEPTEDGQTRFCFYYTLKRSIMTELPPPGVLDRILKQKLGVGMIFEEEMVGRY
                     CPGETKNPFDLESDQGVHCQFNVKMTIPDLDEFVASKDHRVELTGKIKFAEFNGDRDV
                     ICALEEGSYFNYLRMNPETEEREMRYHIRFKHNDRLFLFDGTKFMQKDHKGDINEILE
                     DYTTLYCRVTKSDDGVQVGVAKLKFRTFEDIKSIASLLEFGVSFQVTGTTNLWKKIAA
                     RNKFNAFTMQFIFDEYNPSGL"
     gene            complement(4015..4785)
                     /locus_tag="VI935_01075"
     CDS             complement(4015..4785)
                     /locus_tag="VI935_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24223.1"
                     /translation="MIPTRQSEAKQSNPNLQERIRREEKPRRKIETNTEWVSRVTANS
                     NLEGQVLLLGSTALQDFRIRVAQSHVRQDLLPSFWSHVALFGAKSSARDWLLYEVQLY
                     PNNGFGNVPKENGVQEGKLSKYDDPERFPNIACINFFIKSKTIEEEVSKFRKQRSLID
                     IGSLIVQWLAFIWGVGEYGNPLLKGVGVPSAAFVEGVFAGAGLELTPGLSTRSSCPEA
                     IWQSAKWWHEFYESTATRNKAPKGFYVTDQKSAAVAEE"
     gene            complement(4827..6224)
                     /locus_tag="VI935_01080"
     CDS             complement(4827..6224)
                     /locus_tag="VI935_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24224.1"
                     /translation="MNMILVPHTIDHQKTDLWIAAIGATSPPEGSSVFLMPGEKEIKL
                     SKGNWRTITADGTLKPTLRNVFIQTVTIPNLQPNTTYEVKTKEGTNARFSTLPASLPK
                     EGERPFTVLLASCFYYGNDKEGRMGVAVKLLPEDAKPNVKILCGDQVYLDLPAFAEFS
                     DDEAWLAENFLNKYILTWGQTNGYQTLLRTGGTYFTADDHEFWNNFPNWATLINNTWK
                     EEGRERWKKVALALYKDFQSDDPSKAGHPRRFEIGNLSFFIADTRVFREEGDINFMSQ
                     QDFLQLKQWILGLQGPGVLVLGQPIFHEPARQSNAKLGDRALSNYKPQYTELVRTLFQ
                     AQHSILVLTGDVHYGRVASCELRSSPIPVKLIEVIASPSSLVSTITTGRPEDAPARFP
                     PEPIPGLVHSDIRTLYKTPTRRVLLFKEVSKDHFATLHFTERAGRVRMRVRYWFPKKV
                     SQEDVESDVLDPIDL"
     gene            complement(6485..>6636)
                     /locus_tag="VI935_01085"
     CDS             complement(6485..>6636)
                     /locus_tag="VI935_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HLE24225.1"
                     /translation="SDGLKPILGLDVWEHAYYLKYQNRRADYIGAWWNVVNWEQVAKN
                     YAGAK"
BASE COUNT         1804 a         1352 c         1560 g         1920 t
ORIGIN      
        1 aaacgtaata catccttcat tcggacaggg tgtggtaaag agggtggagg ggttaggtga
       61 cgaagcgaag gtggttatct ctttccatga tcatggggag aaaaagatta tggcaagctt
      121 cctagggctt aaaaagattt agcgtttttt tgttagtttt cctttcttgc tgactgatat
      181 acattattta atttgtaggt tttcctctct atctttatta aagcctggtt gagtagaatc
      241 aacttcttta ttgtatttta catcactagt tttttactcc actccgattt cggaatgttc
      301 atcataatct ttgtctagca tcatgacgtg caaatctggc accctctttt tcataatgct
      361 ttattgattt catattagca aacattgtgg gagttaaaca gttagaaaac tggttttacg
      421 ccttccaggt gaagaaacga tttttatatt tatgtgctag gagcaaaggc tatattttta
      481 aagagttgca ttatattgtt gacaatagtt tatttttctg agacaatggc aatcaagaag
      541 ttggttatgg tcaataagtg ttcgtcaata tattaggaat caatagttat taggtttctt
      601 agaattatga ataagtgagg aaaaagttaa agggagatca actatgaatg gaaccttaac
      661 cagtaattgg aacagtcgtt ctggaaccta tgacgttgtc gtaatcggtt cgggttatgg
      721 aggagccatt acggcctgtc gtatcgccca ggctaagctc aaaccaaaac caaacatatg
      781 tatcctggaa cgtggaatgg aatggatccc cggaatgttt cctgacgagc ctgaggacat
      841 cactcaacat tccctgaccc ctttaaatcc actgggtcta tacgagtatc ggcttttcaa
      901 agacattgat gtgattcaag ggtcaggact tgggggtact tcattggtta acgccaacgt
      961 tgctattatc ccagagccag ctgtattcga gcagaatcca tggcctgtgg ggatcacagg
     1021 taacatgctt gctccttact atgagcgggc ggctaaaacc ttagatgtcg gacaacatcc
     1081 acgcggaatg gatctgctca aggttcgtgc actaaagaag cgagctgatc tttcgccaaa
     1141 tgcaaagttt gatctactca gaatagctgt aaacttcaag gttgatggga ttgatccaga
     1201 aaccggtgta gaaagacatc cctgcattga ttgtggcgat tgtgttactg gctgtaatgt
     1261 ccgtgctaaa aatacccttt atatgaacta tctgccgctt gctcgaaaag caggagtgga
     1321 aatttttact caggttaaag tactttttat aaaagccgat ccaaatggtg gatatcagat
     1381 tcattatgaa cgattcactc ctcgtcacaa tttgcctgaa actggtatta ttaaagccaa
     1441 acgggcagtt atagtctcag ctggttcact aggttcgaca gagattatgt tgcgatcaaa
     1501 gaatcagggg ctcaaactgt ctgacactgt ttgtactcgc ttttccggca acggagactt
     1561 ttttggtatt tcatacaacg gtaatcagcg gacggatatc ctgggttttg gcaatcatcc
     1621 taacgacccc ccgcggtcta acgtgaaagc cgggccaacg attgtggggg taatccgata
     1681 cgatcgcaat aaaccactta aagaacagat taccgtagag gacttatcgg ttccgcgtgg
     1741 aggtgtaatt ccaacgcgga aagtcctaag tactctttca gccggaggca gggatacgga
     1801 ccctggagac ctagtccagg agctagcaag gattgctaga gacatccagt ttgatgcagg
     1861 gggagcatta aaccactcta tgatatatct agtcatggga catgatgatg ctggtggaac
     1921 catgaacttg aaaaacgaca ggctacagat cgactggccc ggtgtcggag accaagatgt
     1981 attcggccgg attaattcgg agctcttcca gcacgccaaa gcccttggcg ctacttttat
     2041 cgaaagtcct gtgtggagct tctttcatag ccgccgtttg atcacagctc atcctcttgg
     2101 cggctgtcca atgggagaga caagtcattt aggttcggtt gacgaatgcg gtcgggtcta
     2161 caatggcaag agtgacgaag aaatacatcc agggctgtat attgccgatg gatcagtcat
     2221 tcccacttcc attggtgtga acccatttct tacaatctca gcgctttccg aatacatcgc
     2281 tgagtgtatc gtaaaggaac ttaaaaaata agatgtgata cacactcagg gttattcagg
     2341 acaagttaag gaggaatgaa tcatgaacgt caaccatctg gaagtcattg gttatcgaga
     2401 ggatcagctt aaaaagatat tcgagtcctc ggagaccctg tctatggaca ttatgatcaa
     2461 cagtggcaag cgcaagattg atcgtagaaa ccgaatcatt tataacgacg tatactggaa
     2521 gggctttttc ccagtaggcc atgttttgaa tctcttctcc agcaacttcc acacaggttt
     2581 caagaagcgg ttatttaagg aggccgggca cattgctggt gttacaagtg attctgatgg
     2641 attcattaac gccaggaatc tcttggaggt gaaaaaggat agtcagctgg gcaagcacat
     2701 attgcttaga tacattgaaa gcccatggca ggggttctac gacatcttta aggtgttaag
     2761 cgacaaactt cttattggaa gggttttttt gggaccatat cctcacggga ccgagatgtt
     2821 tacattctgc atggttcgtg agtacacctt tgatgacatt actgtagacg accaccgtga
     2881 actctttgac actgcaaaga taccaaaact ggaagagatt gaaggtgtct gggatatggc
     2941 tgcggttagc aatagcaatc accgtgcagg ggttgctaga cttgagtttg acgtcaagcc
     3001 tgatcatcgg atcgaaggac gctaccagtt catgagtttg gtacaaggac aaatgagaac
     3061 agagctgaca gaggatgaat tacgaatgca cgacttcacc cccttgcacg acgagattcg
     3121 tagtatagat gtagattaca tgataggcaa atggtgtagt gctagaaagg agacttttgg
     3181 cccgctatcg gcaggccttc tccagttcga acctactgaa gatggtcaga cacgtttttg
     3241 tttctactat acgcttaaga gatctataat gacggagcta ccaccacctg gtgtccttga
     3301 tcgtattcta aaacagaaac ttggtgttgg catgatcttt gaggaagaaa tggttggccg
     3361 ctactgtccg ggagagacaa agaacccatt tgatctggaa tccgatcagg gtgttcactg
     3421 ccagtttaat gttaagatga ctattccaga ccttgatgaa ttcgtcgcaa gtaaagacca
     3481 cagagtagag ttaacaggta agattaagtt tgctgagttt aacggagatc gtgatgtaat
     3541 ctgtgccctc gaagaaggta gttatttcaa ttacctgagg atgaatccgg aaacagagga
     3601 gcgggagatg aggtatcaca tccgcttcaa acacaatgac cgattgttcc ttttcgatgg
     3661 tacaaagttc atgcagaagg accataaggg tgacatcaat gagatattag aggactacac
     3721 aacgctctac tgtagggtta ctaagtcgga tgatggtgta caggtaggtg tggcaaagtt
     3781 gaaattcaga acctttgagg atattaagag cattgctagc cttcttgaat ttggcgtctc
     3841 cttccaggtt actggaacga ctaacctatg gaagaagata gccgctagaa ataagttcaa
     3901 tgcctttaca atgcagttta tattcgatga atataaccca tcgggtctgt aggaataggt
     3961 tttgtggtca tttgattgag ctaaggacta tgtcgagatc ataagtattt tatctcattc
     4021 ttctgcaacg gctgcggatt tttggtcagt gacataaaaa cctttaggcg ccttatttct
     4081 tgtagctgtt gattcataga attcatgcca ccattttgcg gattgccata tggcttcagg
     4141 acagctagac ctggttgata ggcccggagt tagctcaaga cctgctcctg cgaatacgcc
     4201 ttcgacaaag gcagcacttg gaactcccac gcctttgaga agggggttgc cgtattctcc
     4261 tactccccat ataaaggcta gccactgaac gattagagaa ccgatgtcta taaggcttcg
     4321 ttgtttacgg aatttgctaa cttcttcctc gatggtttta gatttgataa agaagttaat
     4381 acaggcgatg ttaggaaacc tctcagggtc atcatactta gagagctttc cctcctgaac
     4441 tccattttcc ttaggaacat ttccgaaacc gttatttggg tatagctgca cttcatatag
     4501 gagccaatcc ctagcactgg acttagctcc gaataatgcc acatgggacc agaaactagg
     4561 aagcaagtcc tgacgcacgt gtgactgagc aacccgtatt cggaagtctt gtagagcagt
     4621 acttccaaga agtaaaacct gcccttctaa attggagttg gccgttacac gagaaaccca
     4681 ctcggtgtta gtttctatct ttcgacgagg tttctcctct cggcgtatcc tctcttgtag
     4741 gtttgggttt gattgcttag cttctgattg tcttgtaggt atcatgttag catcctcctt
     4801 tttttggtat tgtctctagg tctccattat aaatctattg ggtctaatac atcggattca
     4861 acgtcttctt ggctcacttt cttgggaaac caataacgaa cgcgcatacg gacgcgccca
     4921 gccctttcag tgaaatgaag tgtagcgaaa tggtcttttg atacttcttt gaaaaggagg
     4981 actcgcctcg taggtgtctt atagagtgtt cttatatctg aatgcactag tcctggaatt
     5041 ggttctgggg ggaatctcgc gggcgcgtct tcaggtcttc ctgtagttat ggtgctgact
     5101 agagaagatg gcgatgcaat aacttctatt aatttaaccg gtattgggct gcttctcaat
     5161 tcacacgagg ccacacgtcc atagtgtacg tctccagtta gcaccaatat tgaatgctga
     5221 gcctggaaga gtgtgcgaac gagttctgtg tattgcggct tataattgga cagggcccgg
     5281 tctcctaatt ttgcgttaga ttggcgggca ggttcatgaa atattggttg accaagcact
     5341 aatacacctg ggccttggag acctagaatc cattgtttca gctggagaaa atcttgctgg
     5401 ctcatgaaat ttatgtcacc ctcttctcga aatacacgtg tgtctgcaat gaaaaaagag
     5461 aggttaccta tctcgaagcg tctaggatgg cctgcttttg atggatcgtc agattggaaa
     5521 tctttatata aggcaagtgc cacttttttc catctctctc tgccttcctc tttccaggta
     5581 ttgtttataa gggttgccca attgggaaag ttgttccaga actcatggtc atctgcggta
     5641 aagtaggtac ccccagttct aaggagtgtt tggtacccat tggtttgtcc ccacgtcaga
     5701 atatatttat tgaggaagtt ttctgccagc caggcctcgt catcggaaaa ctcagcaaaa
     5761 gccggtaggt ctaaatagac ctggtcaccg catagaatct tgacattggg tttggcgtcc
     5821 tccggcagaa gcttaacagc tacacccata cggccttcct tgtcgttgcc gtagtagaaa
     5881 catgaggcaa gtaggaccgt gaaaggacgc tcaccttcct ttgggagcga tgctgggagc
     5941 gtagagaacc gcgcgtttgt accttcctta gttttaactt cataagtagt attcggctga
     6001 agattaggga tcgtcacagt ttgtataaag acattacgga gtgtaggttt caaggttcca
     6061 tctgctgtga tggttcgcca attccctttt gaaagcttga tctctttttc tcccggcatt
     6121 agaaaaacag acgagccttc aggaggcgag gtagccccta tagcagcgat ccaaagatcg
     6181 gttttctgat gatcaatagt gtgaggtact agaatcatat tcatgatacg aatcagttta
     6241 gagatccact tttaagagaa aagctagact aacatggtgg ataagtcaaa cccgatccta
     6301 ctataatttc tatgtatgaa gaaacagata gacggttaat tgtcttcctt gtttaacttc
     6361 caaatatctg gataaagaaa ggtgtttatt gtataaaggt gttaaatcgc tctttattgc
     6421 ctaattaagc taagccgacg gcgtttcacc gccgtgttat tgtcaattta aaatcttaaa
     6481 gtatttactt tgcaccagcg taatttttgg ctacctgttc ccagttaact acattccacc
     6541 atgccccaat atagtctgca cggcggtttt gatatttaag atagtaggcg tgctcccaaa
     6601 cgtccaaacc aagaataggc ttaagaccat cggaaa
//
LOCUS       DATDRL010000027        14635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1263167, whole genome shotgun sequence.
ACCESSION   DATDRL010000027 DATDRL010000000
VERSION     DATDRL010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14635
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263167"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..697
                     /locus_tag="VI935_01090"
     CDS             407..697
                     /locus_tag="VI935_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24226.1"
                     /translation="MTLVTSILSLENISITAKMLLIVLHCNTDSEEKCWLSDYEIARL
                     LAMHPKSIQKVLGELVRRKLIARVYDELRRRYIIPQGYEWETPLELQVGPQQ"
     gene            942..1229
                     /locus_tag="VI935_01095"
     CDS             942..1229
                     /locus_tag="VI935_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24227.1"
                     /translation="METQRLHLFACVQCGRVNEHACEVQGDNPNEVHYYHHEDSDKKP
                     HSVEISRNAKGEKNYSLKACGKTMEEAKVIAYAFMLETDVIIGKGDQGGKK"
     gene            1226..2017
                     /locus_tag="VI935_01100"
     CDS             1226..2017
                     /locus_tag="VI935_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24228.1"
                     /translation="MSDKDKSTSLAHVEESVFAIMDSLDDDLVIAEIEKRVVDTWVYH
                     FPAKEGPEIWGLSKKGVDQALEELEKQGLVFEEDFCSVVLDPSDSDYLLFSAKVNKYR
                     YDRNLNRIHLGSLTGYKRQWIREKRKDGSLTTDRFYFEKGCAKALRNAKSRAISVELE
                     SKIIAFAKDKGKVKDVENKPHKPDISPEEEEIQGLRDTRLKNRLRKVFAQHKRLGLND
                     KEKVKENIASMIKIPKDFSLAKFVEDDKLYNEYQDLMDLQMSEKA"
     gene            2018..2305
                     /locus_tag="VI935_01105"
     CDS             2018..2305
                     /locus_tag="VI935_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24229.1"
                     /translation="MDQFFKGWSVFIFVPTKRRADDTALGYHVIAENLIVAKNMLGAY
                     FQRECPLCSITLCRLHSDLTLQKEAPLTRMVVCWKTGDKISQGQVVKYGDA"
     gene            2534..2755
                     /locus_tag="VI935_01110"
     CDS             2534..2755
                     /locus_tag="VI935_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24230.1"
                     /translation="MAIVRVRRWGRTLAFTIPHGYARECGIEQRDVYEVLMTKTGLLY
                     RKIEKKPNLKRSLLPYAKDGKAIIEGGET"
     gene            2752..3576
                     /locus_tag="VI935_01115"
     CDS             2752..3576
                     /locus_tag="VI935_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HLE24231.1"
                     /translation="MTAVYNQTQFQLIPIYAIVPAEREIRETMSKEGLEELIEDIKRR
                     GLINPVTVKPLHDGKFEVIAGMRRFEACKQAGWYEIPCIIREDSVIEAEYTKLAENFK
                     REDVNPFDEGKYYQRLVDELGLTPKDIAEATNRSMGYIHARLDLIAGDPTLASAVQDG
                     IIGLGVGLELNKLPTPEKRTFYLQYAIDYGATLRLVQQWVRDETTSHPWTGQEVVGVT
                     SPLADFQPSTLQYECEVCGEMLVTNEVYTIRSSAQCKADVLRMRQILKEQEKRGKV"
     gene            3563..3979
                     /locus_tag="VI935_01120"
     CDS             3563..3979
                     /locus_tag="VI935_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24232.1"
                     /translation="MEKCNHITYTIVKKWKRAAAMIKPRKQINTNRKGSKFVDKVKKY
                     LEDRGYIVIKVSASKPFDLVAWIKDIQVAMLFECRTQGSVAQAFRDLWKASLGIDADR
                     FVVKPRGKTLQVWQARTHGHAKIELYVLTEDFRVAN"
     gene            4123..4278
                     /locus_tag="VI935_01125"
     CDS             4123..4278
                     /locus_tag="VI935_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24233.1"
                     /translation="MADPNDMEKLLQRFSTALPLDVTPPVVEECVVVEFEDGTVDCIP
                     VSELERA"
     gene            4371..4553
                     /locus_tag="VI935_01130"
     CDS             4371..4553
                     /locus_tag="VI935_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24234.1"
                     /translation="MGLAKGIIPEVFWDKSKKEEVIRVLINSPIPNDLKQIALKEWAN
                     EVGSDITEMDRKRVGL"
     gene            4663..5175
                     /locus_tag="VI935_01135"
     CDS             4663..5175
                     /locus_tag="VI935_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24235.1"
                     /translation="MPRPPGRFYRTQDLPGGYNYPHMLPRERDMFNIWLNEFGQSFEG
                     FMFDVRLGSGRPAQLQDIREVERKLGYKFSLNQEEQDRFDGQIRIAWQSLTQKRADSI
                     GFKGDETWLIEVKKVFNMSLIGQLDVYSVLLLRDYPPDGSFRRVAIVGDVDPDVGPIA
                     QERGIMIFRV"
     gene            5188..5379
                     /locus_tag="VI935_01140"
     CDS             5188..5379
                     /locus_tag="VI935_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24236.1"
                     /translation="MSVERIYCGIILEGDREIKSTFALCQFIAIAINAISANGIYVHP
                     VIGQENGKTCLYIDKETTK"
     gene            5376..5771
                     /locus_tag="VI935_01145"
     CDS             5376..5771
                     /locus_tag="VI935_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24237.1"
                     /translation="MSWKEVEATREGLVGKKTACGLVIRTKSIFVALPHWKALFKIVL
                     LAYDGKPYLAPVLDVGPWNEEDEQYVFGELRPLAEHGVGKYRKPTNKAGIDLSDGLIR
                     FMGFDPKDWGKRNVLWRFVEPQEVHLLPT"
     gene            5993..6976
                     /locus_tag="VI935_01150"
     CDS             5993..6976
                     /locus_tag="VI935_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24238.1"
                     /translation="MKRYIQIASQVASASGTHLWVNGGPLSGSGTLPFQGGLASDEWL
                     YGGFVIASLVTSLQTLDQLSGIVIHCLAYTESEVNQLWLSTPLPTPVPSPTPTPTTFI
                     GVAGCPPKLTIPVPSPPYRWYTSDEWSVASQLFPVPSAPLRPLGLGAYLIDSKDSVAI
                     QEASQAGGKLSVIIVENPTQWFLWYVVVTCPEGVTPTPTPTPTPTPTPTPTPTPTPTP
                     TPSPIPIIIPPPTGYLIFPVVITIYSFADQQAREIVEEIIRLLANIEPKRFPDPYGFQ
                     TDRELIRALLDQLRSLINAGLISAVNVPGLLYFIDQVSYIVNVLLTISPDP"
     gene            7186..7608
                     /locus_tag="VI935_01155"
     CDS             7186..7608
                     /locus_tag="VI935_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24239.1"
                     /translation="MPAIKSSAEIAGKWGRVTPQRSQDYQAGVTSPKNSWQEKTGAAE
                     QSWAQGTAEAASSGRFGRGVAQAGDAKWKRKTLDLGVQRWPDGVRKSETDFQTGFNKY
                     REVIQSTQLPPRGPKGSPTNNERARIMAQVLHEAKVRG"
     gene            7673..8476
                     /locus_tag="VI935_01160"
     CDS             7673..8476
                     /locus_tag="VI935_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24240.1"
                     /translation="MGKAFELIAGFVTAPSTTFTALTMATGSSLTVRNAPLGSEVRLL
                     QCWSDQQTAGNIRIRSPKLHDNVQGIRLFSVASEVEPLFPMGSYEKLIPQDILVIEMT
                     GSATASDIESFVALMYYSDLPGTDARLITPEEAMRRMSHIVTIENTLALGTAGGFSGE
                     EAINAEFDLLKANTDYALVGYAVSAECAAVRWRGADTGNLGVGGPGNELRKSMTAEWF
                     ILLSHAYMLPLIPVFNSANKGAVLLDGVQDENGTDVTVTSIFYELGMPR"
     gene            8490..9281
                     /locus_tag="VI935_01165"
     CDS             8490..9281
                     /locus_tag="VI935_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24241.1"
                     /translation="MHIQKPFYVSQDDKLVVSSIDFSDLTPIVIGVRLLRDDGTLEIM
                     QYSHIPSGDRTRRTTEYPLNKGYLLSVTASLLGATTKRGEAFVQVYLFYGLVASNLAT
                     ALLISDYVDGTFSPSWPSGVVRNSLDGMGMLKRFAGTNPAAGVEIVVTVPTGAYWRII
                     SFRAQLVTDATVATRQVHLLFDDGATTLFFRPAQTTQIASLTRNYDALSMGYEAVAVL
                     PQIAIELPPNLILPAGYRIQTATDNFAAGDNWGSPQLFIEEWLEL"
     gene            9376..9708
                     /locus_tag="VI935_01170"
     CDS             9376..9708
                     /locus_tag="VI935_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24242.1"
                     /translation="MIISSGAIILPFNTAQIIIRAWAQLTTGTATTTVTPRIRRGTAV
                     TGTLVGEENAENVKAAAGSTEPFFIIAAEGRSNERSVEYSLTLEQASATGDGSVLFAA
                     IEVEVLTG"
     gene            9709..9906
                     /locus_tag="VI935_01175"
     CDS             9709..9906
                     /locus_tag="VI935_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24243.1"
                     /translation="MDILIAISGAQLITLLGMAGKIWSWSHKLEARLSVLETHVMTLL
                     KRDEARMWYLQGLNREVKKDV"
     gene            9899..10165
                     /locus_tag="VI935_01180"
     CDS             9899..10165
                     /locus_tag="VI935_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24244.1"
                     /translation="MFKYLKYLSLLPILISMIGHMEELFKGIKEGEVKKNAVMAALSN
                     LFSMLSSAGVLGKEDIDKIFPNMPKLVDLIVDFLNSFGVFKHGS"
     gene            10155..10361
                     /locus_tag="VI935_01185"
     CDS             10155..10361
                     /locus_tag="VI935_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24245.1"
                     /translation="MEVNIIKIQEISVFTAAGILKAQIQVSFNVGEHGPFTILIPKEE
                     FDNTRATREIEKIKREVEAVSKRE"
     gene            10430..10987
                     /locus_tag="VI935_01190"
     CDS             10430..10987
                     /locus_tag="VI935_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24246.1"
                     /translation="MEGLLALPASGCDVVNYIRANKNIILSWATHLSQPESPDPCSQA
                     EASLFNFWRNFLSYGAAQIITADNCIYDAVRNANGYITGIQDKVNKPSVYADGVKCFG
                     TIEKYPPPAKTTECAQASDINKFTVLRSSLEYYIIDILQLWLTAKSLYEKAITICDGV
                     SDDEQCQSCMCEAVRQTEFLLAKLI"
     gene            10987..12153
                     /locus_tag="VI935_01195"
     CDS             10987..12153
                     /locus_tag="VI935_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24247.1"
                     /translation="MACIDCCNQIIELLNQIGSKVDGLLSRLRNTELKLAKVESVVTP
                     VIPYQNELDPIIAYFLDKLRTSKTYRLILLDRVVTSLRETGKDNRFITVSILSDALGK
                     WLLDCFLAFLAALPKELRIDPPTNGSGEPEYITVLTFGQLGKELEIKEALLASQVNLD
                     GGTSERTGGTEAYGASVGSTGLGLGQILGAYTFGLAEGVAKGLKAMMAKGFKIELPDP
                     DEIDPHWGRDKNSSFAFGQFPSEITPEQLAEVTATSRAQVAKNDILVTKTRTGTIQMT
                     PIPFGFTPFLTKKEGDVTSFTNITDPTATPIQRQVLPDGSILYTNINPLDDPFVTFTD
                     ADGVYHATNLQPGSDPFYVDVLPDGSSLYSNVPYTDIPFTTSIDENGTIIITQK"
     gene            12227..12460
                     /locus_tag="VI935_01200"
     CDS             12227..12460
                     /locus_tag="VI935_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24248.1"
                     /translation="MAFIGAFLVGIKEEPIEFYEDTFETVATDIIKGIANEALEIADK
                     GLPEMQLLVDKLMGPTEVILKKLVDAVLGGTKL"
     gene            12457..13827
                     /locus_tag="VI935_01205"
     CDS             12457..13827
                     /locus_tag="VI935_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24249.1"
                     /translation="MKTLFARLFKEFLTLPLTGGLQVVKRAVLAFETIANKTFEVFAW
                     VVVGRILLNLITDSTLTIPEQFKASLGKLVALQTGNQGLADKVTSSTGFDTFAALAQG
                     QGEFFMGGMLDLFTPEGPITPEIGMQNAQKFVGLNVLVNISAWLTKVMGDILSLGKFE
                     SLGGLPLQISWSLGLGWLTWVIMGPPLQMFIADPLVEGLNRKHFPYRLQLSQILDIFT
                     EGWKDQEWFIDQMGGLGIDEERALLLYEARKKKFSRQQGKRFFERGLIDKQQLREMIK
                     KEGWTDGELDLIMQELINERTFDLTEDIAKEAQKLFEDGQISETSAKTFLTNAHWQPE
                     EIDLAIQLANMKKLRTDLTPSQMLNMMSEKLFTQDQVLKRLKDRGFDDPDAQALIQYE
                     LITAGRGFGGGTVTPEKELTQAQILNAFRNGIILEADAVKRLEEQGLESEDVQILLEL
                     NRPTTQ"
     gene            complement(13832..14110)
                     /locus_tag="VI935_01210"
     CDS             complement(13832..14110)
                     /locus_tag="VI935_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24250.1"
                     /translation="MKQSASLVARSKISFQQLWSKEGWLTEGSQALSNIIALSQAHNR
                     TLPSSPLILSIQEGRIRAYLFPNLWKHKDSIKVNDPESTGDNGIKTPT"
     gene            complement(14082..14369)
                     /locus_tag="VI935_01215"
     CDS             complement(14082..14369)
                     /locus_tag="VI935_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24251.1"
                     /translation="MNLDGPLFLSLISVYSFIAGCMFTGVLTGRRLTKMVTGDRSGFA
                     LGMYIWSLILSAIWPLTLLITLLRRNRQKVDIAELERMARLLHETIRKPGS"
BASE COUNT         4313 a         2916 c         3437 g         3969 t
ORIGIN      
        1 agcctgaatt tcttttaggg aaggttttag tttttctttt tttgaaatat ttatacctac
       61 actgccttac ccttcccttt gggaagggct ttagcgaagc tctgagaacc caagatccat
      121 ctgggtagac tatgccatta gagccaaaag ggaaggggta gtaattttag gtgggaattt
      181 tttaaggaaa gaaaaaacag agtttgagct gtttggatat actctcattg agaggttata
      241 ctcttttttt tttgaaagtc aataggtttt gtaaaaatta tctaagtcgt cgtatgtact
      301 ggacaatgaa ttttataaaa accccttgac agataatagc cagcatggta gtaattaggc
      361 tactatgcag gcgaagatta cccaatccgc tatacagagg tgtgttatga cattagttac
      421 gtcaattcta agccttgaaa acatatccat aaccgctaag atgctcttga ttgttttaca
      481 ctgcaacact gacagtgagg agaagtgctg gctttcagac tatgaaattg ctaggcttct
      541 agctatgcat cccaaaagca tacagaaggt tttaggagag ctagtacgta ggaaattgat
      601 tgccagggtc tatgatgaac tgagacgtag gtacataata ccacaagggt atgaatggga
      661 aacaccgctc gagctgcaag tgggtccaca acaataatta ttttattatt ctaaaaatat
      721 cctcttgaca cactaaattg acgttgatat actgtttcgc agaataaaaa tgcaggagga
      781 cctagagatg cctaaaatat ttttgctcgc aacaagtcct tacagtcccc taatacttga
      841 gcttttgctt gtttgtacgc cgaggtggta cgaggttcgt agtacgggac tgcaacaact
      901 gggcaggtgg cagtattacc tggttgggga ggtgagctaa aatggaaaca caacgattgc
      961 atctgtttgc gtgtgtacag tgtgggcgcg tcaatgaaca cgcttgcgaa gtacaagggg
     1021 ataaccctaa cgaggttcac tattatcatc atgaagatag cgataaaaag cctcacagtg
     1081 tggagatttc acgtaatgcc aagggtgaga agaactattc acttaaagct tgcggaaaaa
     1141 cgatggaaga ggcgaaagtt attgcttatg cgtttatgtt ggaaacggat gttattatag
     1201 gaaagggtga tcaagggggt aaaaaatgag tgataaggat aaatctacta gcttggcaca
     1261 cgtggaagaa tcagttttcg ctataatgga ctccttagac gacgatcttg ttatagctga
     1321 aatagaaaag agggttgttg atacttgggt ttatcatttc cctgcaaagg aaggccctga
     1381 gatttggggc ctgtctaaga aaggcgtaga ccaggctctt gaggagctag aaaagcaagg
     1441 gttggtgttc gaggaggatt tttgcagtgt ggttttagac ccttccgatt ccgattattt
     1501 attgttttcc gctaaggtga acaaataccg ctatgaccgt aatttgaatc gtatacactt
     1561 aggctcactc acaggataca aaagacagtg gattagagaa aaacgcaaag atggctcact
     1621 taccacagac cgcttttatt ttgaaaaagg atgcgctaag gccctacgaa atgcaaagtc
     1681 aagagcaatt tcagttgagc ttgagtcgaa aataattgcc tttgccaagg acaaagggaa
     1741 ggtaaaagat gtagaaaata aacctcataa acccgatatt tcaccggaag aggaggaaat
     1801 acaaggcttg agggatacaa ggcttaaaaa tcgcttacgc aaggtctttg ctcaacacaa
     1861 gcgcctgggc ctaaacgaca aggaaaaggt taaggagaat atcgcctcta tgataaaaat
     1921 ccctaaagac tttagcctgg ccaagtttgt tgaagatgat aagctctata acgagtatca
     1981 ggatttgatg gatttacaaa tgtcggagaa ggcataaatg gatcaattct ttaaaggatg
     2041 gtcggtcttt atttttgttc ctacaaaaag gagagcggac gatactgctc taggctatca
     2101 cgttatagct gagaatttga ttgtagcaaa gaatatgtta ggagcgtact tccaaaggga
     2161 gtgtccgctt tgcagtatca cgctgtgccg attgcatagc gacttgactc ttcaaaaaga
     2221 agcaccactt actcggatgg tagtgtgttg gaaaacgggg gataaaatct cacaaggaca
     2281 ggtggttaaa tatggcgatg cttagtacgc acattctttt agcgataagc ctctcttttt
     2341 tggctgtcgc ggttgtcaat attatcataa gtatctttaa cttagctatt ctaccgtatt
     2401 tacttgaaaa acctactata tggaaatggt gtcttaattt aggggacaaa aagctcttag
     2461 gtggtttggt tcttgtggat attcttatat ggtcgggagt ttttgtgtct ctaacgtggt
     2521 tgggggtgga ttaatggcga tagttagagt gagaagatgg ggcaggacat tagcgtttac
     2581 aatccctcat ggctatgcca gggagtgcgg tatcgagcag agggacgttt atgaggtctt
     2641 aatgacgaaa actggcttgt tatacagaaa aatagagaaa aaaccgaatc tcaagcgcag
     2701 tcttttaccc tatgcaaagg atggaaaagc aataattgaa gggggtgaga catgaccgcc
     2761 gtttacaatc aaacccaatt tcaattaatt cctatatatg caatcgtgcc ggcagagaga
     2821 gagatacgtg aaactatgtc taaggaaggg cttgaagagc tgatagagga cattaagcgt
     2881 aggggcctta tcaatcctgt caccgttaag cctttacacg atggtaagtt tgaagttatt
     2941 gcagggatgc gaaggtttga ggcgtgcaaa caggcaggct ggtatgaaat accctgtata
     3001 attagagagg atagtgtcat agaggcagaa tataccaaac tcgcagaaaa ctttaaacga
     3061 gaggatgtta atcctttcga cgagggcaaa tactatcaaa ggctggttga cgagctcggg
     3121 cttactccga aggatattgc agaagctact aataggagta tgggatatat tcatgctagg
     3181 cttgatctta ttgctggcga ccctactctc gcgagtgctg tccaggatgg aataataggc
     3241 ttaggtgtag ggttagaact caataaacta cctacacctg agaaaaggac gttttattta
     3301 cagtatgcta tagactacgg agctacctta cggttggtcc aacaatgggt acgcgatgaa
     3361 actacgtctc acccctggac tggtcaagag gttgttggtg ttacctcgcc gcttgcagac
     3421 tttcagcctt ctactttgca atatgagtgt gaggtttgcg gcgagatgct ggttacgaat
     3481 gaggtctaca cgattaggtc tagcgctcag tgtaaagcgg atgttctcag aatgaggcag
     3541 attttgaagg agcaagagaa gcgtggaaaa gtgtaatcat attacatata ctattgtaaa
     3601 aaaatggaaa agggcagccg ctatgattaa gcctcgtaag cagattaaca ccaaccggaa
     3661 gggtagtaaa tttgttgata aggtgaagaa ataccttgag gatagggggt atatagttat
     3721 caaggttagc gcgtctaagc ctttcgacct ggtagcctgg ataaaagata ttcaagtggc
     3781 tatgcttttt gagtgtcgga ctcaagggag cgtggcgcag gcattcaggg atttatggaa
     3841 ggcctcgctc gggattgatg cagataggtt cgtcgtgaaa cccagaggaa aaacactaca
     3901 agtttggcaa gctagaacgc atggtcatgc caaaatagaa ctatacgtgc taactgagga
     3961 ttttagagtt gccaactaaa aaagaactaa ctcaaagctt gattgattca caaattcaca
     4021 taaatcagaa ggtagcggat agttttaagg tcacgattcc gacttcctcc tctcgtgata
     4081 cgtttctgtc cgttaccttc aaagaaggtg agagggtata tcatggcgga tccaaacgat
     4141 atggaaaagt tattacaacg attttcaacc gctctaccac tagacgtaac gcctccggtg
     4201 gttgaggaat gtgtcgttgt ggagtttgaa gatggaactg tagattgtat tccggtttca
     4261 gaattagaga gggcttaaat gattgacggc gtaataattt tagcatccgt tttgatagcg
     4321 tgttttgtgc tgttaggctg gacgatgcta tttcataggt gaggtgagcg atgggtctag
     4381 caaaaggaat aatccccgag gtgttctggg ataagtctaa aaaagaagag gttatcaggg
     4441 tattgataaa ttcccctatc cctaacgacc tcaaacagat agccctgaaa gaatgggcta
     4501 atgaggtggg gtctgatata actgagatgg atagaaagag ggtaggacta taattatgga
     4561 aggaataaat cttacaacta ttgattatgt gttcttcggt ttgggcatag gcggattatt
     4621 atttatggtt tttcacgcat tatttatcag gggatagcaa acatgcctag acctccagga
     4681 cgcttttata gaactcagga cctcccaggc ggatataact atcctcacat gctacctaga
     4741 gaaagggata tgtttaatat ctggctaaac gaattcggtc aatcctttga aggattcatg
     4801 tttgacgtaa gattaggctc aggtagaccg gcacaactcc aggacataag agaggtagag
     4861 agaaagctag gttacaagtt ttctcttaat caagaagagc aagaccgctt tgacggacag
     4921 attagaattg catggcagag cctaactcag aaaagagcgg acagcatcgg gtttaaagga
     4981 gacgagacat ggctgataga ggtaaaaaaa gtattcaata tgagcttgat aggacagcta
     5041 gacgtttact cggtgttgtt gttacgagac tacccgccgg atggctcctt ccgcagagtg
     5101 gcaatcgtag gagacgtaga tccagacgta gggccaatag cacaagaaag aggaataatg
     5161 atatttagag tgtagaggag aaatcaaatg agcgtagaaa gaatatattg tgggattatc
     5221 ctagaaggag atagggagat taaatcgact tttgcactgt gtcaatttat agctattgcg
     5281 ataaatgcta tttcagcaaa tggtatttat gttcacccag tgataggcca agaaaatggg
     5341 aaaacctgtt tatatataga taaggaaact acaaaatgag ctggaaagaa gtcgaagcga
     5401 cacgtgaagg gttagtaggg aaaaagacag cgtgtggtct tgttatcaga actaagagca
     5461 tctttgttgc gctgccacat tggaaagcct tgtttaaaat cgtgctactt gcctacgatg
     5521 gcaaacctta cctggctccg gtcctggacg taggcccctg gaatgaggaa gatgagcaat
     5581 acgtatttgg agagcttaga cccttagcag agcatggagt agggaaatac cgcaagccta
     5641 ccaataaggc tggtattgac cttagcgacg gtcttatccg ttttatgggc tttgacccta
     5701 aagattgggg caagcgcaat gtcttatggc gttttgtaga gcctcaagaa gttcatctac
     5761 ttcctaccta gatacttttc ttttctactt agttacaatg ttctgaatga ttcctaagaa
     5821 agatcaaatc cttgtgattc ctgtacccca gccctccgct ggtcaaaatt ttagttatac
     5881 cttaccctcc ggctatatct ataaattgat ttcagttcag tatacgttaa ctacgtccat
     5941 acaagctgcg tcacgcttta tgaggttaga tatatcttat ggtgggatac ttatgaaaag
     6001 atatatacaa attgcatctc aagtagcctc cgcctcggga acacatctct gggtaaatgg
     6061 cggcccactg tctggtagtg gaacactgcc ctttcaagga ggtttagcaa gtgatgagtg
     6121 gctttacggt ggatttgtaa tagcgtcact tgtaactagt ttgcaaactt tagatcagct
     6181 gtcaggcata gtaattcact gtttagcgta tacagaaagt gaagttaatc agttgtggct
     6241 ttctactccg ctacctactc cagtaccttc tccaactcca actcccacca cttttatcgg
     6301 tgttgcagga tgtcctccca aattaactat accagtcccg tctcctcctt atagatggta
     6361 cacatcagac gagtggtctg ttgcttcaca gctttttcct gtgccgtcag ccccattgag
     6421 accactcggg ctaggggcat atttaataga ttcaaaggat tctgtagcaa ttcaagaggc
     6481 ttcacaagct ggtgggaagt tgtccgttat tattgttgaa aaccctacgc agtggtttct
     6541 ctggtacgtt gtagttacgt gtcctgaggg agtgacgcca acgccaacgc caaccccaac
     6601 cccaacacca acgccaacac caacgccaac cccaacgcca accccaacgc cttcacctat
     6661 tccgattatt attccccctc caactggata cctaattttt cctgtcgtta ttacgatcta
     6721 ttcgtttgcg gatcagcaag ctagggaaat tgtagaggaa attatcaggt tgcttgccaa
     6781 tatagagcct aaacgcttcc ctgaccctta tgggttccaa actgataggg aacttattag
     6841 ggctttattg gatcagctaa ggtctttaat aaatgcagga ctaatcagcg cggtcaacgt
     6901 cccaggtctt ttatacttca tagaccaggt ttcttacata gtcaatgttt tattgacaat
     6961 ttctccagat ccttaagcag gtggtcggta tggttatcgt atgagcccca tacactcatt
     7021 aagtgggttc gattcccaca cctgcaataa caattatctc ttgacattcc cttctaattc
     7081 cgatattact taaaataagc ctagagaaga aggcaacaac gcttctctag gccttttttt
     7141 ttgttgcctt ctctctagga aatctaggct agggaggcaa caaagatgcc tgcaatcaaa
     7201 agctccgctg aaatagcggg caaatggggt cgcgtgaccc cacaaagatc acaggattac
     7261 caagcagggg taacatcccc taaaaattca tggcaggaaa agacaggtgc agctgaacag
     7321 tcttgggcgc aaggcacagc cgaagccgca agctcaggaa ggttcggtcg tggcgtggct
     7381 caagccggtg acgctaagtg gaagagaaag actcttgact taggcgttca gaggtggccc
     7441 gatggtgtga gaaagtccga gacggatttt cagacagggt tcaacaaata tcgtgaggta
     7501 atacagagta cacagttgcc cccacgtggc cctaaaggct cacctaccaa caacgagaga
     7561 gctagaataa tggctcaagt cttgcacgaa gctaaagtcc gtggataacg gagttttaaa
     7621 tgcgttgtat atacaacgca tagcgatata aatttattcg gagggaattg aaatgggtaa
     7681 agcatttgaa cttatcgcag gatttgtaac agctccgtct accacgttca cagcactaac
     7741 gatggcaacc ggaagctctt tgacggttag aaacgctcca cttggctctg aagtacgatt
     7801 gcttcagtgt tggtcagatc agcagacagc cggtaacatc cgtattaggt cgcctaaatt
     7861 acacgacaac gtccagggca taaggctttt cagcgtggct tctgaggtgg agccattatt
     7921 ccctatgggt agttatgaga agttaatccc tcaggacatt cttgtcattg aaatgactgg
     7981 atctgctact gccagtgaca tagaatcttt tgtagcactc atgtactact cagacttgcc
     8041 aggcacggat gcaaggctga ttactccaga ggaagctatg aggcgtatga gccacattgt
     8101 cacgatagaa aacaccctag cgttaggcac cgctggaggc ttctcagggg aagaggctat
     8161 aaacgctgaa tttgacctct tgaaagccaa cacagattac gccctggttg gatatgccgt
     8221 tagcgctgaa tgcgccgctg taaggtggag aggcgctgac actggaaacc tgggtgttgg
     8281 aggccctggc aacgagctta gaaagagcat gactgctgag tggtttattc tgctttcaca
     8341 cgcttatatg cttcctttga taccagtctt taactcagcc aacaaaggcg ctgtgctatt
     8401 ggatggtgtt caggacgaga acggaacgga cgtgactgta actagcatct tctatgagct
     8461 aggaatgcct agatagtcaa ggaggtgttg tgcatattca aaaacctttc tacgtctctc
     8521 aagacgataa gctagttgta tcttctattg atttttctga cctcaccccg atagttatcg
     8581 gggtacgctt actgcgtgac gatggcaccc tggagataat gcagtatagc catataccta
     8641 gcggagaccg taccaggcga actactgaat acccgcttaa taaagggtat ttactttctg
     8701 ttacagcgtc tctattgggt gctacaacca agcgtggaga ggcatttgta caagtttatc
     8761 tattctatgg acttgtggct tcaaaccttg ccactgctct tcttatttcc gattatgtgg
     8821 atggaacctt ttctcctagt tggccaagtg gcgttgtaag aaattcactt gatggcatgg
     8881 ggatgcttaa aaggtttgct ggaactaacc ctgcggctgg cgtagaaata gttgttacag
     8941 tgccgacagg agcttattgg cgtattatct catttagggc acagttagtt accgatgcaa
     9001 cggtggctac aagacaagtg catctattgt ttgatgatgg cgcaactacc ctttttttcc
     9061 ggccagcaca gacaacacaa atcgcttcct taacaagaaa ttacgatgcg ctaagtatgg
     9121 gatatgaggc agtggcagta cttccacaaa ttgccataga gttgcctcca aacttgatcc
     9181 tgccagcagg atataggata cagacagcca cagataattt tgcggctggt gataactggg
     9241 gtagtcctca attatttatt gaggaatggc tagagcttta atcaatggta tggaggtttt
     9301 atcatggcag acatcctaga gaaaattatt aatttttcag gtaccgccgt aaccatcaca
     9361 accacgactg agagggtgat catttcaagt ggcgctatca tcttaccttt caacacagcg
     9421 caaatcatca tcagagcatg ggctcagctc acgactggga cggcaaccac gactgtaact
     9481 cccagaataa gaagaggcac agccgtcacc ggcacgctag taggagaaga aaacgctgag
     9541 aacgtaaaag ccgccgctgg atccacagag ccattcttca taatagccgc tgagggtcgg
     9601 tcaaatgaac gatcggttga atattcactt acactagagc aggcatcggc aaccggagac
     9661 gggtcggttc tttttgctgc aatagaggtg gaggtcctaa caggataaat ggatatcctc
     9721 atagcaattt caggagcaca gcttataacc ttgctaggga tggctggtaa gatttggagt
     9781 tggtcgcata aattagaggc ccgattatca gtgctagaga cgcacgtaat gacgttgctt
     9841 aaaagagacg aggctcgtat gtggtattta caaggcctaa acagggaggt taaaaaagat
     9901 gtttaaatat ctaaaatact tatcgctatt gccgattcta atttccatga ttggtcacat
     9961 ggaagagctt tttaagggca ttaaagaagg tgaagtaaaa aagaacgccg tcatggcggc
    10021 tttatctaat ctcttctcta tgctctcaag cgctggtgtc ttaggcaagg aagatataga
    10081 taaaatcttt cctaacatgc caaagctggt tgacctcata gtggactttc taaactcatt
    10141 cggagtcttt aagcatggaa gttaatatca ttaagattca agagatttct gtttttactg
    10201 cggccggcat tctcaaagct caaatacaag tttcattcaa cgttggtgag catggcccct
    10261 tcactatact cattcccaaa gaagaatttg acaatactag agctactaga gaaatagaga
    10321 aaatcaaaag ggaagttgaa gcggtatcaa agcgtgaata atggcatttt ccctccctcc
    10381 cacggcgcca gcctgtgatc aaggttggtg taccattgtc aaacctttaa tggaaggact
    10441 tctagcattg cctgcgtccg gatgtgacgt agtaaattat attcgtgcaa ataaaaatat
    10501 tattctttcg tgggctacac atcttagcca acctgaatca cctgatccct gctcgcaagc
    10561 ggaagcctcc ctgttcaatt tttggaggaa ctttctaagc tatggcgctg ctcaaatcat
    10621 aacggcagac aactgtatat atgatgcggt taggaacgct aatgggtaca tcacagggat
    10681 acaagacaag gtaaacaagc ctagtgtcta cgctgatggc gttaaatgtt ttgggactat
    10741 cgagaaatat cctccacctg caaaaactac tgagtgcgct caggcgtcag atataaacaa
    10801 atttaccgtt cttcggagtt cgctagaata ttacataatt gatatattgc agctttggct
    10861 taccgcaaaa tctttgtatg agaaagctat cactatctgt gatggtgtca gtgacgatga
    10921 gcaatgtcaa tcttgcatgt gtgaggcggt tagacaaacg gagtttttat tggctaaact
    10981 aatataatgg cgtgtataga ttgttgcaat caaataatag aacttttaaa ccagataggt
    11041 agcaaggtag acggccttct atcccgatta cgaaacacgg aactaaaatt agccaaagtc
    11101 gagagtgtcg tcaccccagt aattccctat caaaatgagc ttgaccccat tatcgcttat
    11161 ttcctcgaca aactccgcac aagtaaaaca tatcgcttga tactgctcga cagggtcgtc
    11221 acctctctac gtgagaccgg aaaagataat cgctttataa cggttagcat cctatcagat
    11281 gcgttgggaa aatggttact ggattgtttt ttagcctttc ttgcggcgct acctaaagag
    11341 cttcgcattg acccacccac gaatgggtca ggtgaacccg aatatatcac tgtcctcact
    11401 tttgggcaat taggcaaaga gcttgaaatc aaagaggctc tcttagcttc tcaggtcaat
    11461 cttgatggtg gtacaagcga gagaactgga ggaacggagg cgtatggcgc aagcgttgga
    11521 tctacaggcc tggggttagg tcagattctt ggggcgtata cttttggatt agctgaaggt
    11581 gtagcaaaag ggcttaaagc aatgatggca aaaggtttta agattgagct ccctgatcca
    11641 gatgaaattg atccgcactg gggaagggat aaaaacagta gctttgcatt tggtcaattc
    11701 ccatctgaga ttactcccga acaacttgca gaggtaaccg ctacatccag agcacaggtg
    11761 gccaagaacg acatacttgt aaccaaaaca cgtaccggaa caatccaaat gacaccaatt
    11821 ccttttggct tcaccccctt cttgactaaa aaagaaggtg acgtcacatc ttttacgaat
    11881 ataactgatc ctacagccac acctattcag cgacaagtgc tccctgacgg ctcaatactc
    11941 tatactaaca tcaatccact tgacgatcct tttgttactt ttactgatgc tgacggtgtt
    12001 tatcatgcga ccaacttgca accaggctcc gacccctttt atgtcgatgt tttaccggat
    12061 ggttcaagtt tgtatagcaa tgtgccctat acagacatac cttttacaac ttctatagat
    12121 gaaaacggga ctataattat cacacaaaaa tgacatggct aaattcgtaa tcaaaaatcc
    12181 gaagacagta ataactgacg ttctaagcaa ctatgacgcg tggatcttgg catttattgg
    12241 agctttcctc gtagggatta aggaagaacc tatagagttt tatgaagata cgtttgaaac
    12301 ggtggctact gacattatca aaggcattgc caatgaagcc cttgaaatag ccgacaaggg
    12361 gctacccgag atgcaacttc tagtagacaa acttatggga cctacggaag tcattctcaa
    12421 gaagctcgtt gatgcggtgt taggaggcac aaaactatga aaaccctatt cgctagatta
    12481 ttcaaggagt ttttaaccct tcctttgacc ggcgggctac aagtagttaa aagagcagtg
    12541 ctggcatttg agactatcgc taataaaacc tttgaggttt tcgcttgggt ggttgttggg
    12601 aggatcctgt taaatctaat cactgactct accctaacca ttccagaaca atttaaagca
    12661 agcttgggaa aattagtcgc gctccaaaca ggcaatcagg gtctagccga caaagttact
    12721 tcctccaccg gcttcgacac ctttgcggct cttgcgcaag gtcaaggtga gtttttcatg
    12781 gggggtatgc tagatctatt tactccagaa ggccctatca ccccagaaat aggaatgcaa
    12841 aacgctcaga agttcgtagg cttgaacgtt ctagtgaaca tttcggcatg gcttactaaa
    12901 gtgatgggtg atatcctttc actaggtaag tttgaatctc ttgggggctt gccacttcag
    12961 atctcctgga gtttagggtt aggttggctc acctgggtaa ttatgggacc gccactccaa
    13021 atgttcatag cagaccctct cgtcgagggc ttaaacagaa agcattttcc ctatcgttta
    13081 cagttatccc aaatcctcga catctttaca gaagggtgga aagatcaaga atggtttata
    13141 gatcaaatgg gtggtttagg tattgacgaa gaacgtgccc ttctgcttta tgaggctagg
    13201 aaaaagaagt ttagtagaca acaaggtaaa aggttttttg aacgtggttt aatagacaaa
    13261 caacaactcc gcgagatgat taaaaaggaa ggatggacgg acggagagct tgacttaatt
    13321 atgcaggaac ttataaatga gagaactttt gacctaactg aagatatagc gaaagaagct
    13381 cagaaacttt ttgaggacgg acagatctcc gaaacatccg ccaaaacatt tctaacaaac
    13441 gcccactggc agcctgaaga aatagattta gcaattcagc ttgctaatat gaaaaaactt
    13501 aggactgacc ttaccccttc acagatgctt aatatgatgt ctgaaaagct atttacacaa
    13561 gaccaagttt tgaaaaggct aaaagataga ggctttgacg acccagatgc tcaagcttta
    13621 attcagtatg agcttatcac agcgggtaga ggtttcggag gagggaccgt gacccctgaa
    13681 aaggagttaa cccaagctca aatcctcaac gcatttagaa atgggattat acttgaagct
    13741 gatgcggtaa aaagattaga agagcagggg ctggagagtg aagatgttca aattttactc
    13801 gagcttaatc gtcctacaac gcaataaaac cttacgttgg ggtttttatg ccgttatctc
    13861 ctgtagattc cggatcgttg acctttatgc tatctttatg tttccataaa ttaggaaaga
    13921 gatatgctcg gattcttccc tcttgaatgc ttagaattag gggtgagctt ggcaacgttc
    13981 tgttatgggc ttgggatagt gcaattatat tcgacagggc ttgactacct tcagttaacc
    14041 aaccctcttt actccatagt tgttggaatg atattttact tctagctacc aggcttgcgg
    14101 attgtttcat gaagtaacct cgccatcctc tccaactctg caatatctac tttttgacgg
    14161 tttctcctta atagagttat caaaagagtt aggggccata ttgcactgag gataagactc
    14221 caaatataca tacctaatgc gaagcctgag cggtcaccag taaccatttt ggtcaagcgc
    14281 ctacccgtga gtacacctgt aaacatacac cctgctatga agctataaac gcttataagg
    14341 ctgaggaaaa gcggaccgtc taagttcatc taacacctcg ttttgaaaat ggagtaaaac
    14401 actatgcaca atttcaccgt accctaaaga tgagtttcgt agatcaagct ttgggtctgc
    14461 taagactcta taccttatgg aagtcataat ctccagaagc tcacctatat ctgacggtct
    14521 taattgcata acttcagtaa atcacatctg aaagtttttt gcaagggctt ttgaaaagtt
    14581 ctgactggtc aaactaagaa cgaagagata tattcttttg atgcccgaag tggca
//
LOCUS       DATDRL010000028         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3905259, whole genome shotgun sequence.
ACCESSION   DATDRL010000028 DATDRL010000000
VERSION     DATDRL010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3905259"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..963)
                     /locus_tag="VI935_01220"
     CDS             complement(124..963)
                     /locus_tag="VI935_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HLE24252.1"
                     /translation="MKRSVLGKGLDALIPAEKVGQGYVILGIDEIRPNTFQPRKDFDE
                     EAINELAVSIQEKGIIQPIVVRKNINAYEIIVGERRWRAAQRVGLTKIPVIIKDVSDR
                     EALELALVENLQREGLNPIEEASAYGQLIEDFGLTHEEISKRIGKERSTVTNQLRLLK
                     LPEEVREALIKGEITAGHARALLGLESPNKMKEVLEAIRKDKLSVRKTEKLVQKLLGD
                     KQTSIKSHDIDPYIKHLTDELKKVLGTQVKIIDKGGEGKIEIEYYSKDELERLIEILA
                     RGS"
     gene            complement(960..1748)
                     /locus_tag="VI935_01225"
     CDS             complement(960..1748)
                     /locus_tag="VI935_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLE24253.1"
                     /translation="MGKIISVANQKGGVGKTTTAVNLAASLASTHRMTLLIDFDPQGN
                     ATSGIGIDKEKIEKSIYHAIIGDVNIKETIIEVQHEGIKGYLRVSPSNPDLTGAEIEL
                     INLEGREGRLKEAIKPIVDEYDFILIDCPPSLSLLTVNALTAAHSVLIPVQCEYYAME
                     GLGQLERTLSLVKQRLNPELEVEGYVLTIFDSRNKLCHMVANEVRDFFKDHVFQTIIP
                     RSVRLAEAPSHGKPIILYDEKSIGAISYISLAKEIIERNGGQIL"
BASE COUNT          525 a          421 c          346 g          711 t
ORIGIN      
        1 ggctttagtt ttacttctat ttttactcta aactctttca tgtcgtgaat cgtgaatcgt
       61 gaatcgtgaa tcgtgtatca ggcctatatc actaaccaaa aaccacgaat cacgaaaccc
      121 gaatcacgaa ccccgcgcta agatttcaat aagtctttca agttcatctt ttgaataata
      181 ctctatttca atctttcctt cgccgccttt atctataatt ttaacctggg taccaaggac
      241 ttttttgagt tcatctgtta ggtgtttgat ataggggtca atatcatggg acttgattga
      301 cgtttgctta tcccctaata atttctgaac aagcttttca gtttttctta cggagagttt
      361 atcttttcta atagcttcaa gaacctcttt cattttattt ggagattcta aacccaggag
      421 ggctctcgca tgccctgcag taatttcgcc tttaattaga gcctctctaa cctcctcagg
      481 aagcttgaga agtctaagtt gatttgttac agtagaccgc tcttttccga ttctttttga
      541 tatctcctca tgagtgagac caaaatcttc aatgagttgg ccgtaagcac tagcctcttc
      601 tatgggattt agaccctctc tttgaaggtt ttcaacaagt gctaactcta gggcttctct
      661 atcagataca tcctttatta tcactgggat cttggtaagg ccaactcttt gcgcagccct
      721 ccaccttctt tcaccgacaa tgatttcata ggcatttatg ttctttctta caacgatagg
      781 ctgtataatc cccttttctt gtattgatac agcaagctcg tttatggcct cttcatcaaa
      841 atcctttctt ggttgaaatg tgtttggtct tatctcgtca atccccagga tcacataacc
      901 ctgcccaact ttttcagcag gaataagagc atcgagaccc ttacctaaga cgctcctttt
      961 caaagtattt gtcctccatt cctttctata atttctttag caagagaaat gtaacttata
     1021 gctcctattg atttttcatc gtatagaatt atgggttttc catggctagg tgcttctgcg
     1081 agcctaacgc ttctgggaat tatagtctga aaaacgtggt ctttaaaaaa gtctcttacc
     1141 tcatttgcca ccatgtgaca gagtttgttt ctcgaatcaa atattgtaag cacgtaacct
     1201 tcaacctcga gctcagggtt taatctctgc ttaacgagag aaagggttcg ttctagttgt
     1261 ccaagccctt ccattgcata gtattcacac tgaactggta taagaacgga atgggcggcg
     1321 gttaatgcgt ttactgttag aaggctaagc gaaggtgggc agtcaattag tatgaaatcg
     1381 tattcatcaa ctatcggctt tattgcttct tttaacctcc cctcccttcc ttctaggttt
     1441 ataagctcaa tttctgctcc tgtgaggtca ggatttgatg gtgagactct gaggtaacct
     1501 tttatacctt catgttgcac ttctataatt gtctctttta tatttacatc accgattata
     1561 gcatggtaaa tactcttttc aattttttct ttgtctattc cgatgccact tgttgcatta
     1621 ccctgtgggt caaaatcaat aagaagtgtc atcctatgtg tggacgcaag tgatgctgcg
     1681 agattcactg cagttgttgt ttttcctact ccgcctttct ggttagctac agatattatt
     1741 tttcccatta actatgacct tgatgataaa ccctctaaat atttacgtca accttttaat
     1801 cgggagtttc atataatgag ttcatagaag gatttgtcat cttatttttt tttagattat
     1861 gcattcaagt ttatcataac tataagaaaa aaattaaagc taaaaagaaa aagtattgca
     1921 tcgtttcaaa taaaaattta taatttaagt gagtaaacaa accttcaacc ctaaagggtc
     1981 tttgtcaagg gagatattgg gtg
//
LOCUS       DATDRL010000029         4151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1807897, whole genome shotgun sequence.
ACCESSION   DATDRL010000029 DATDRL010000000
VERSION     DATDRL010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807897"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VI935_01230"
     CDS             <1..339
                     /locus_tag="VI935_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HLE24254.1"
                     /translation="AKDEMHHAEWLIQRILFLEGTPIVSKLNPIKVGTTVLEIITGDQ
                     DAEISAVRAYNDAIALAHEVGDQASVDLLIRILKMEEDHVDWAEMQRAQIEQMGLENY
                     LTNQTRGAKD"
     gene            complement(627..1820)
                     /locus_tag="VI935_01235"
     CDS             complement(627..1820)
                     /locus_tag="VI935_01235"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HLE24255.1"
                     /translation="MKLAARAERLKPSATLAITAKAKALKAEGVDVVGFGAGEPDFDT
                     PENIKEEAIRAIKEGFTKYTAVGGIDELKNAIIHRIKEDHGIKYEKSEIIVSCGAKHT
                     LYNLSQAIFEEGDEVIIPSPYWVSYPEQVILAGAKPVFIETNEDYGFKIDPDELKKRI
                     TKKTKALILNYPSNPTGATYTEDELRDIVDVAMKAGLMIISDEIYEKIVYDGLTHTPV
                     VKLGEDIKKSTILVNGVSKSYSMTGWRIGYAAGDKRIVEAMSNLQGQSTSNPTSISQR
                     AAVEALKGSQEEVKRMAKEFEKRKNYIVERLNKIPGFRCFNPQGAFYVFPNISSFFGK
                     KYAGKEIRNSVDFTEFLLGEAKVAVVPGIEFGADSYVRISYATSMEEIKKGVDRIEKA
                     LEKLK"
     gene            complement(1836..2321)
                     /gene="coaD"
                     /locus_tag="VI935_01240"
     CDS             complement(1836..2321)
                     /gene="coaD"
                     /locus_tag="VI935_01240"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HLE24256.1"
                     /translation="MKKLAIYPGSFDPFTNGHLNIIERGVKVFDNVIVAVAHNVSKKT
                     IFTLDERVEIIREIFKDRPEVRVDYFQGVLVEYAKKLGTNIVLRGMRTVSDFEYELQM
                     ALSNKMLYPELETIFMVTESPYSHISSSLIKEIISLGGSAKEMVPKIVDKKLRKKLFR
                     K"
     gene            complement(2318..2869)
                     /gene="rsmD"
                     /locus_tag="VI935_01245"
     CDS             complement(2318..2869)
                     /gene="rsmD"
                     /locus_tag="VI935_01245"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HLE24257.1"
                     /translation="MLQVLTGEAKGRKLKVPKGRTVRPTTSRIKKTIFDTLGDISGFK
                     VLDIFAGSGGLGIEALSRRAAHVTFIEKNPLVYRILRENVSLCGFLDRTNLICAHYEG
                     ALKRLKKSGKKFDLIFIDPPYILYRRKQVGDFIQQLSELLEESGTIVIEHNYRIENSI
                     KGFKRITKPFGETNLSLFRRGDE"
     gene            complement(2997..3767)
                     /locus_tag="VI935_01250"
     CDS             complement(2997..3767)
                     /locus_tag="VI935_01250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HLE24258.1"
                     /translation="MKTASSIISLLILTSALFLGITMRTHSEALPEGKMVSYKSDGET
                     VSGFLVTPEGKGPFPAIIVIQEWWGLNDQIKNQAKKLAKEGYVTLAVDLYRGKVTTDP
                     EEAHELLRGLPEDRALRDLISAVSYLKTLPNVTKHKIGSIGWCMGGGYSLSLAINSPD
                     ITACVIYYGRLITDKEQLRRTKASVIGFFGEQDSGIPPSSVNAFEEDMKTLGKNVSIY
                     IYPGAGHAFANEEKPSYNPKAAKDSWDKTLTFFKEKLR"
BASE COUNT         1078 a          926 c          784 g         1363 t
ORIGIN      
        1 gcaaaggatg agatgcacca cgctgagtgg cttatccagc gtatactctt tctagagggc
       61 acaccgattg tctcaaagct caatccgatc aaagtcggca caactgtcct tgaaatcatt
      121 acaggcgacc aggatgctga aatttcagcc gtgcgtgcct acaatgatgc catcgccctg
      181 gcgcacgaag tgggcgacca ggctagtgtt gacctgttga tccgcattct caagatggaa
      241 gaggatcatg tggattgggc agaaatgcaa cgtgcgcaga ttgagcagat gggtctcgag
      301 aattacctca ccaatcaaac aaggggcgct aaagattagc ttcttccgca tgaataggaa
      361 aaggatgggt aaatatccgc gtactttaaa ccttgtgcgg cccaacactg catgcaccct
      421 acgcagctac cggctcgtcc cagcgaaggc tcatgtagtt ccagcaagtc ttgcccgcga
      481 aggagtcttt gacaaccgcc gcgagggtga tcctggccat tagaacctta caggcatcgc
      541 aagcatcgaa gtgaatggat tagaaatata agttgcatca ctcttatttc ataatgtaag
      601 aatcgaatgc tttttcatcc gtttccctac ttaagtttct ctagagcctt ttctatccta
      661 tcaactccct tttttatttc ttccatggag gttgcatagg atattctaac gtagctatct
      721 gctccaaatt cgatacctgg tacaactgct accttcgctt cccctaaaag gaattcggtg
      781 aaatctacgg aatttctgat ttccttacca gcatatttct ttccgaagaa cgatgaaata
      841 tttgggaaca cataaaatgc accctgtggg ttaaagcatc taaaaccagg gattttgttt
      901 aatctttcaa ctatataatt ctttcttttc tcaaactctt tagccatcct tttgacctct
      961 tcttgagacc cctttagcgc ttcaactgcc gccctctgag aaatcgaggt tggatttgat
     1021 gtagactgcc cctgaaggtt actcatggcc tcgactatcc ttttatcacc cgccgcataa
     1081 ccgattctcc atcccgtcat tgaataagat tttgaaacgc cgtttacaag aatcgtagat
     1141 ttttttatat cctctcctag cttcacaact ggggtatgtg taagtccatc gtaaacaatc
     1201 ttttcataaa tctcatctga aataatcata agccctgctt tcatcgctac atcaacaata
     1261 tctctaagtt cgtcttcggt ataagtagcc ccagttgggt ttgaggggta attaagtatt
     1321 agggcttttg ttttttttgt tatacgtttt ttgagctcat ctggatcaat cttaaaacca
     1381 taatcctcgt tggtttctat aaatacgggc tttgcgccag caagaatcac ctgctcaggg
     1441 taggaaaccc agtaaggcga cggaattata acctcatccc cttcttcaaa aatggcttga
     1501 gataaattgt aaagcgtatg ttttgctccg caggatacaa tgatttcaga tttttcatat
     1561 tttattccgt gatcctcttt aattctatga atgattgcat ttttaagctc gtcgataccc
     1621 cctactgccg tgtattttgt gaacccttcc ttaattgccc ttatcgcttc ttcttttata
     1681 ttttctgggg tgtcaaaatc cggctctcct gcgccgaatc caacgacatc gacaccttca
     1741 gccttgagcg cttttgcctt tgcggtgatt gcaagggttg cagatggttt tagtctctct
     1801 gcgcgggcgg cgagtttcat tcgagttgta acctcctatt ttctaaaaag tttctttctg
     1861 agttttttat caacaatctt tgggaccatt tctttagctg aaccacctag ggatattatt
     1921 tccttgataa gtgatgagct tatatgtgaa tatgggctct ctgttaccat aaatattgtt
     1981 tcaagctcag ggtagagcat tttattagac agcgccattt gaagctcgta ttcgaaatcc
     2041 gagacagttc tcataccacg gagcacaatg ttagtgccta gtttttttgc gtattctacg
     2101 agtacgcctt gaaagtagtc taccctcacc tcggggcgat ctttgaagat ctctcttatg
     2161 atctcaactc tctcatcaag tgtaaaaatc gtcttcttag acacattatg tgcgacagca
     2221 acaattacat tatcaaaaac ctttacacca cgctctatta tatttaagtg cccatttgta
     2281 aaaggatcga aggagccggg ataaatcgca agctttttca ttcatcacct cttctaaaga
     2341 ggcttagatt ggtttctccg aaaggttttg taattctttt aaagcccttt attgagtttt
     2401 ctatccgata attatgctcg attactatcg ttccactctc ttcgagaagc tcggatagtt
     2461 gttgtataaa atccccgacc tgttttcttc tgtataaaat atatggcggg tcaataaata
     2521 tgagatcaaa cttttttccg cttttcttaa gccttttgag cgctccttca taatgggcgc
     2581 agattaaatt tgttctatcg agaaaaccgc aaagagatac gttttctctg agtatcctgt
     2641 aaaccagtgg gtttttttca ataaatgtga cgtgagctgc tctccggctc aaagcctcta
     2701 taccaagtcc tcccgaacct gcaaaaatat cgagcacttt aaacccgctt atatcaccaa
     2761 gggtgtcaaa gatcgtcttc ttaatcctgc ttgtagttgg gcgaactgtt ctccccttcg
     2821 ggactttgag ctttcttccc tttgcttcac ctgttagcac ctgcaacatc ggcttgatat
     2881 actaccatgc taactagttt tggacaaaaa tttattgatt cgtggctcgt gaatcgtgac
     2941 tcgtgattcg tggctcgtgt atcgaattgc gaactgccat tcacgattaa tgggatttat
     3001 ctcaatttct ctttaaaaaa ggtaagggtt ttgtcccatg aatcctttgc tgctttcgga
     3061 ttataagagg gtttttcttc gtttgcaaag gcgtgtcctg ctcctggata gatgtagata
     3121 cttacatttt ttccgagtgt tttcatatct tcttcaaatg catttacgct cgatggtgga
     3181 attccactat cttgttcacc gaaaaaacca atgacagagg ccttggttct tcgtagctgc
     3241 tctttatcag taattagcct tccgtaataa ataacacacg ccgtaatgtc cggactattt
     3301 attgcaagcg acaacgaata tcctccaccc atgcaccagc cgatagagcc gattttatgt
     3361 ttcgttacat tgggtaatgt ttttagatag gaaacggctg aaatcaggtc tcttaatgct
     3421 ctatcctcgg gtaaccctct taaaagctca tgcgcttctt caggatcagt tgtcacctta
     3481 ccccggtata aatcaaccgc tagcgtaacg tacccctctt ttgccagttt ttttgcttgg
     3541 ttttttatct gatcatttag tccccaccat tcctgtatta ctataatcgc aggaaatggt
     3601 ccctttccct caggtgtaac tagaaatcct gaaactgttt ctccatctga tttgtagcta
     3661 accattttgc cttctggcag tgcttctgaa tgcgttctca ttgtgattcc caaaaacaat
     3721 gcggatgtta gaatcaaaag ggaaataata gaagaggccg ttttcatatt acatacctcc
     3781 ataaaaagtt ctatgcagca gtgatttaaa gtttatacgg ccaagagtat aattgctata
     3841 atattttctg ctcccaaaat gattagtatc tggctacctt gctgccctaa taatagttct
     3901 gtgtagtttt gcacagtcca cacagtgtaa taatacacag tattgaacat ctttattgtc
     3961 atcaagttca ttccatttat aacttcttgt aagcatttaa tattgaaaat gttaaaccgc
     4021 tggtatggat cttgcctatt atagcttttg gttatgaaac cttattgcgt ataactggtt
     4081 cttagataat aaggctgaat gtaggaggaa aggtaaatga aaaaattctt tctagtatta
     4141 attcttatag t
//
LOCUS       DATDRL010000030         2668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2852287, whole genome shotgun sequence.
ACCESSION   DATDRL010000030 DATDRL010000000
VERSION     DATDRL010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2852287"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /gene="obgE"
                     /locus_tag="VI935_01255"
     CDS             <1..889
                     /gene="obgE"
                     /locus_tag="VI935_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HLE24259.1"
                     /translation="VVARKNMSSLLDHHYKQHYKAKNGEHGRGKDQHGKNAPVLLVPV
                     PVGTVVKDFFTREILGDLKENGQILIVAKGGRGGRGNARFATSTNRAPRYAEPGEEAE
                     ERTLLLELKLLADVGIIGFPNAGKSTLISRISAARPKIADYPFTTLIPNLGVVSYNEG
                     KTFVVADIPGLIKGAHEGTGLGIKFLRHIERTKVLIHLLDLSPLTERDPIEDCHVMNK
                     ELEAYSPDLIRKPQIIAPNKIDITEAREKLKGIKTYFKKLGIEVFPISSATGEGLKRL
                     IEEVGKRLERLKNINTLHD"
     gene            complement(1322..1483)
                     /locus_tag="VI935_01260"
     CDS             complement(1322..1483)
                     /locus_tag="VI935_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24260.1"
                     /translation="MSEIKNGKMELNIAGQIIEKWWLELPHKFSNIIIDECIIMPDHL
                     HGIIIIVET"
     gene            1789..>2668
                     /locus_tag="VI935_01265"
     CDS             1789..>2668
                     /locus_tag="VI935_01265"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HLE24261.1"
                     /translation="MRKYLGTFIACFLALIAVSLSVNSALGESNNQIVRISKIKIKGA
                     DHVSIKGIKERIGTEFPSIRPWAKDPEFDEEILKDDMVRIQRLYADHGYYDAKAEYKL
                     KYNNKRDKVEITIKIIEGQPVILKDLNTDFLEDLNEDVKKDILDSLPIKVDKTFSSIK
                     YQQAKLVISAILSNNGYPKPVIEGEAIVNRKEKWAKVSLKINPGLLYRFGSIIAEGNE
                     KVESYVITREATFKKGDIYSTKELDDTQTRIFQLGLFSSVLIDTDLKEETTTADVKVK
                     VRERKLGEVKIGAGFGT"
BASE COUNT          931 a          489 c          523 g          725 t
ORIGIN      
        1 agtcgttgca agaaaaaata tgtcttccct tttagatcat cattataagc aacactataa
       61 agccaaaaat ggagagcatg gaagaggaaa agaccagcac gggaaaaacg caccagtgct
      121 tttagttcct gttcctgttg gaactgttgt aaaagacttt tttacaagag agatactggg
      181 agatcttaaa gagaacggtc agattcttat tgtagctaaa ggcgggcgtg gcggaagagg
      241 aaacgcaaga tttgcgacgt caacaaatcg agccccaagg tatgcagagc ctggagagga
      301 agctgaagaa agaaccctgc ttcttgagtt aaaacttctt gctgacgtag gaatcatagg
      361 atttccgaat gcaggaaagt caacgctgat atccagaatc tcagcagcac gccccaaaat
      421 cgccgactat ccttttacca cgctgatccc aaacctgggg gttgtaagct ataacgaagg
      481 aaagacattt gttgttgctg atattccagg actcataaag ggcgcacacg aagggactgg
      541 gcttggtata aaattcctaa gacacataga acgcacaaag gttcttattc atctccttga
      601 tttatcgccg ttaacagaga gagaccccat agaagattgt cacgtgatga ataaggaact
      661 cgaagcatac agccccgatc taattagaaa accacaaatc atcgcgccaa ataaaattga
      721 cataacagaa gcaagggaaa aactcaaggg aattaaaacc tacttcaaaa aacttggaat
      781 cgaagtattt ccgatttcct ctgcaacagg ggaaggatta aagagactaa tcgaagaggt
      841 agggaaaagg ttagagagac ttaaaaatat caatacatta cacgattaga atcaataacc
      901 ctgcagcaag ctgcagggta tggtcttaaa aatataccgt tatagctgtc attgcgagga
      961 gcaaagcgac gaagcaatct aaaatgagag attgcttcac ttcgttcgca atgacaatac
     1021 actattgtgg aaaccctgta gcaagctaca gggaattctc aagttaaaga gtcggtttca
     1081 ctcataccgg ctttgcctat ttgaggtggt ttttatattc ataggatttt cttcgtccat
     1141 ccaccatttt aagggatttt cggtgatgta ttcacgtata cgatttaatt cctcttcgct
     1201 gcggataata cgttcgtaat aattacgttg ccagaattta ccatgaaacg gcggttatct
     1261 atcacgtttt accctacgaa tgtattcgtt tacggtcatt gttttaatcc attgaataat
     1321 cttatgtttc aacaattatt ataatcccat gaagatggtc gggcatgatg atacattcat
     1381 caattattat gtttgagaat ttgtgaggta attccaacca ccatttttca ataatctgcc
     1441 ctgcgatatt caattccatt ttgccatttt taatctcact caataaattt tccgttatgg
     1501 acgcatatag ttacaagata ggcccacggt tgtgaattat cgtattattt caatcgtatt
     1561 gatcttctat gatgaaaatc aggatcggat ttcatactga cttagcctta ttgaagcata
     1621 atttagatga taagaattct actacccctt tcaaacattt ttccttaatt gattactagc
     1681 acctatatac aaccctctct tgaatattaa tggaagctca gcataataga aaccctaatt
     1741 aagcaattta aatattaaag agaagaaaat gaggttacag cccacctaat gagaaaatat
     1801 ctagggacgt ttattgcttg ttttcttgct ctaattgctg tctctctttc cgtaaactca
     1861 gccctcggtg aatccaataa ccaaatagtt cgaatttcaa aaataaagat aaagggcgca
     1921 gaccatgttt ctattaaagg aataaaagaa aggataggga cagaattccc atcaattcgg
     1981 ccgtgggcta aagaccctga gtttgacgaa gaaatcctaa aagatgatat ggtgagaatt
     2041 caaaggcttt atgcagatca cggatactac gatgccaagg cagaatacaa gctcaaatac
     2101 aacaacaaaa gggataaggt agaaattaca attaaaataa tagaaggaca acccgttatc
     2161 cttaaagatt tgaatacaga ttttctagaa gatctaaatg aagatgtcaa aaaggatatc
     2221 ctagattcat tacccattaa ggtggacaaa accttttctt cgataaaata tcaacaagcc
     2281 aaacttgtaa tctcagccat cctctctaat aacggttacc ctaaaccagt cattgaggga
     2341 gaagcaattg taaatagaaa agaaaaatgg gcaaaagtga gtttaaagat aaatccagga
     2401 ttgctttata gatttggttc aatcattgct gaaggtaatg agaaggttga aagctatgta
     2461 attacaagag aagccacgtt taaaaagggg gatatttatt ctacaaaaga actcgatgac
     2521 acccagacaa gaatctttca actcggactt tttagttccg tactaataga cactgattta
     2581 aaagaagaaa caacaactgc agatgtaaaa gtaaaggtta gagagagaaa actaggtgag
     2641 gttaaaatag gtgcaggatt cggcacag
//
LOCUS       DATDRL010000031         2958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_751984, whole genome shotgun sequence.
ACCESSION   DATDRL010000031 DATDRL010000000
VERSION     DATDRL010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751984"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2769
                     /locus_tag="VI935_01270"
     CDS             <1..2769
                     /locus_tag="VI935_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HLE24262.1"
                     /translation="WGQNVFTDGNQMIVSMDFAKQDDVMLALKDSKWDLCIVDEAHKM
                     SAYKYGEKISKTQRYNFGEHLSPITNYLLFLTATPHRGDPENFRLLLDLLEPGFFADT
                     AMLSQSIQNKDNPLFLRRLKEDLKDFEGAPIFPPRKVKSTKYYLSDDEKKLYNAVTEY
                     VEKHFNKALDKEKRNVTFALTILQRRLASSVRAIRKSLERRYERLNELYKKGKIVQEE
                     GYDEEFIEDLEEKERWKKEEELLEKLTSAETLEELKQEIGKLEELVVIAKEVEKKEIE
                     TKLSELRNVMDAEKLQQSGIKLLIFTESKDTLDYLVEKLRKWGYSVTLIHGGMNLDQR
                     IKAENDFRNQAQIMVSTEAGGEGINLQFCWLMVNYDIPWNPNRLEQRMGRIHRYGQQH
                     EVHIYNMVAVDTIEGKILIKLFEKLSRIKEHLGSDRVFDVINDIVVGKSLKDLIMNAI
                     ANPRTMDDILKDFERIPDEVAINRVKEASLEALATRHIDLTRILGEQRTAKENRLVPE
                     YVEEFFKRSAKVLNIKADKRQDGFWHVVSVPFDIRNQPYDFKTRFGEVQREYSKVSFD
                     KEKAFKGQAEFVAMGHPLLESIVSAILTRYDPVADEGATFIDTEGKKDGILWFFQAEI
                     KDGKNEIAGKRLFTAYQELTKSDQSFSFVNPAILWDLKPLSNQEQPETNKSKPYVPDT
                     NPIISFVISNGLENYKKELLERRLRDAEIKRKYGIRSLELMTLDSDGKIIDYQTRRDK
                     GENIPEVTIQNEVRKKEDLERKKQRLLKEIDAQVHLYPTEPKIIGAVRVIPGKVQGEM
                     IADEEIEQIGMDVTMEYERRQSRVPEDVHLENLGYDIRSIDEARYRYIEVKARAREGA
                     VALTPNEWLMSQRLKEEYWLYIVVNAASKPVLYVIQNPALHLNPQEEISIVRYIVKDW
                     KNKSEKAI"
     gene            2766..>2958
                     /locus_tag="VI935_01275"
     CDS             2766..>2958
                     /locus_tag="VI935_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HLE24263.1"
                     /translation="MNKSFIEVDFPIKQVSEESVREKNIRHGHISTLHIWWARRPLAS
                     SRASIYAALTPEPKDEEERL"
BASE COUNT         1027 a          517 c          671 g          743 t
ORIGIN      
        1 tggggacaga atgtctttac tgacggaaac cagatgattg tatctatgga ttttgccaag
       61 caggacgatg ttatgttagc tcttaaagac tccaaatggg acttgtgtat agtagacgaa
      121 gctcacaaga tgtctgcata taaatatggc gaaaagatta gtaaaaccca gcgttataat
      181 tttggtgaac acctctcacc cattacgaac tacttacttt tcttaaccgc aacgccccat
      241 agaggagacc ctgaaaactt tcgtctgctg cttgacctcc tagaaccagg cttctttgct
      301 gatacagcaa tgctttccca atccattcaa aacaaagata atcctctatt tttaagaagg
      361 cttaaagaag atttaaaaga tttcgaaggg gctccaatct tccctccaag aaaggtaaag
      421 tcgactaagt attatctgtc tgatgatgag aagaagcttt acaatgcggt tacagagtat
      481 gtagagaaac actttaacaa agctctagac aaagaaaaac gtaatgtcac ctttgctctt
      541 actatattgc agcggcgtct tgcttctagt gtccgtgcta taagaaaatc cttagagcgg
      601 agatatgaac gactaaatga actctataaa aaagggaaga tcgttcaaga agagggatac
      661 gatgaagagt ttattgagga tttagaggaa aaagagaggt ggaagaagga agaagaatta
      721 ctcgaaaaac tcacctccgc cgagactcta gaagaactta aacaagagat aggaaaactt
      781 gaggagctcg tggtcattgc taaagaggtt gaaaagaaag agatagaaac taagcttagt
      841 gaacttagaa acgtaatgga tgctgaaaag cttcagcaat cggggataaa attacttatc
      901 tttacagaat ccaaagacac cctagattat ttagtggaga aacttagaaa gtggggctat
      961 tcggttacac tcattcacgg cgggatgaat cttgaccaga ggattaaggc tgagaacgat
     1021 ttcaggaatc aagcccagat tatggtatca accgaggcag ggggagaagg cataaacctc
     1081 cagttttgct ggcttatggt caattatgac atcccatgga atccaaatcg cttagaacag
     1141 aggatgggca gaattcatcg ctacgggcaa cagcatgagg ttcacattta taacatggtg
     1201 gcagtggata caatcgaagg gaaaattctg ataaaacttt ttgaaaaact atcacgcata
     1261 aaagagcatt tagggtcgga cagagtgttt gatgtgatta atgatatagt agtcggaaag
     1321 agtttaaaag atttgattat gaatgctatc gcaaacccca gaacaatgga cgacattcta
     1381 aaggactttg aaagaattcc tgatgaggtg gctatcaata gagtaaagga agcaagccta
     1441 gaagccttgg ctacacgaca tattgatttg acaagaatct taggtgagca gagaacagcc
     1501 aaagagaata gacttgttcc agaatacgtt gaggaattct tcaaaaggag tgctaaggta
     1561 cttaatatca aggctgacaa aaggcaggac ggtttctggc atgtagtttc tgtacctttt
     1621 gatatccgca atcagccata cgacttcaag acaaggtttg gtgaggttca aagagaatac
     1681 agcaaagttt cttttgataa ggaaaaggct tttaaagggc aggccgaatt cgtagcgatg
     1741 ggtcatccac tcttggagtc aattgtaagt gctattttaa cccgttatga ccctgtagca
     1801 gatgaaggtg ctacttttat agatactgaa gggaaaaaag acggcattct ttggtttttt
     1861 caagcggaga taaaagacgg gaaaaatgag attgcgggta aacgattgtt tactgcttat
     1921 caggaactaa ctaaaagcga ccaatccttt tcatttgtca atcctgccat tctttgggat
     1981 ttaaaacctc taagtaacca agaacaaccg gaaactaata aatccaaacc ctatgtccct
     2041 gacacgaatc caatcatatc ttttgtcata agtaatgggc ttgaaaatta taagaaggaa
     2101 ctcctcgaga gacgccttag ggacgctgaa ataaaaagga agtacggaat tcgttctctt
     2161 gagttaatga ctcttgattc agatggcaag attattgatt atcaaacaag aagggacaaa
     2221 ggagagaaca tacctgaggt aacgattcag aatgaggtaa ggaaaaagga agatttagaa
     2281 cgcaaaaagc aacgcctact taaagaaatt gatgcccaag tccatcttta ccctactgaa
     2341 ccaaaaatca ttggtgctgt aagggtgatt cccggcaaag tgcaaggaga gatgatcgct
     2401 gatgaggaaa ttgaacaaat tggaatggat gttacgatgg aatacgaaag aagacaaagt
     2461 agagttcctg aggatgttca tcttgaaaac ctaggctatg acattcgttc tatagatgaa
     2521 gcaaggtatc gctacattga ggtaaaagcg agagcaagag aaggtgctgt tgcattgaca
     2581 ccaaatgaat ggcttatgtc gcagagactc aaagaggaat actggcttta tatcgtggta
     2641 aatgctgctt ctaaaccggt actctatgtc atccaaaatc ctgcacttca tcttaatccc
     2701 caagaagaga taagtattgt gagatacatt gtgaaggatt ggaaaaataa gtcagagaag
     2761 gctatatgaa taaatcattt atagaagtag atttccctat caaacaggta agcgaggaat
     2821 ccgtaaggga gaagaacatt aggcatggac atatttccac ccttcacatc tggtgggctc
     2881 gaagaccctt agcatcttca agggcatcca tctatgcagc actgacacct gaaccgaaag
     2941 acgaagaaga aagattaa
//
LOCUS       DATDRL010000032         6507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_472894, whole genome shotgun sequence.
ACCESSION   DATDRL010000032 DATDRL010000000
VERSION     DATDRL010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472894"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..1622)
                     /gene="mtaB"
                     /locus_tag="VI935_01280"
     CDS             complement(273..1622)
                     /gene="mtaB"
                     /locus_tag="VI935_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HLE24264.1"
                     /translation="MKRVSFVTLGCKVNQYDSAAILNTLPEGRYSEVSFPEEADIYVI
                     NTCTVTQKADAEARHYISRAKRINPDGVVIVTGCYAQVSPSELEKVNGVDYIVGNFDK
                     FSLKEAIQDAKPQPQPRVIVSDIFKVREFESPEIKNFPERTRAFLKVQDGCNYRCTFC
                     IIPYARGRSRSLPVIEVLRRMEVLAQSGYKEVVLTGVHLASYGRDIGTKLLKLLEEIE
                     KAKIIKRVRLSSLDPADTADELINFVASSGTICPQFHVALQSGDGDVLKRMRRRYTPD
                     KFLHCTNLIRKKMPDAAIGTDIMVGFPGETDEEFENSFNLAKESTLTYFHVFPYSKRK
                     GTPAAIMRNQVHPQVIKDRSSLLRELGQKKKTEFYRSFIGRRFTVLVERPVLSRSPER
                     SEGSVEGGSRGTTPNYISVRILDGDFKIGDEVHVEIRDVKGKEALGLPESTVNPQMG"
     gene            complement(1689..2756)
                     /gene="mnmA"
                     /locus_tag="VI935_01285"
     CDS             complement(1689..2756)
                     /gene="mnmA"
                     /locus_tag="VI935_01285"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HLE24265.1"
                     /translation="MKKRILVAMSGGVDSSTTAAILKEEGHEVIGVTMQLWDYGDGEG
                     GCCSADDVRDARMVAEEIGIPHYVVNYMDVFKKYIVEDFINKYLSGRTPNPCVLCNQF
                     MKFNFLLRRAMELGADYLATGHYARVETDEEGKRFYLCKANDESKDQSYFLFALTQNE
                     LKRLIFPLGSMTKSEVRNIAKSMDIKVANKPDSQEVCFITGGDYREFLKAHAEVKNGK
                     GEIVDINGNVLGNHDGVFSFTVGQRRGLGISKGKPLYVIKVDLESNKVVVGEENDISR
                     TTLMASDVIWINESPSDEIRVKTRIRHRHRESDSVVRMKSDGEALVEFKDPQRAITPG
                     QAVVFYEGNRVLGGGWIKEVL"
     gene            2961..3185
                     /locus_tag="VI935_01290"
     CDS             2961..3185
                     /locus_tag="VI935_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009544311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLE24266.1"
                     /translation="MSYKVSVVIEKDEYGYYAYCPELEGCQTQGGSLDEVMSNIKEAT
                     ELYLETLSEEERKELLSKEILTTALEVKVA"
     gene            3178..3369
                     /locus_tag="VI935_01295"
     CDS             3178..3369
                     /locus_tag="VI935_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002779783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HLE24267.1"
                     /translation="MPKLPRLTSKEAEEILLKDGFKLIRSKGSHRIYMKGNKRIVIPF
                     HTGITLHPKIVKQVLKCRK"
     gene            3478..4890
                     /locus_tag="VI935_01300"
     CDS             3478..4890
                     /locus_tag="VI935_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLE24268.1"
                     /translation="MRVLLIQPQQGTRFGISKIVTTEPLGLESIASPMLLDGHDVRII
                     DLKVEKPSRLDEELKKFSPEVVGISCSFTTDVYPTIEVANHIKGFNKKIFVFIGGHHA
                     SLLPSDLLVPSVDAIAIGEGEITGKELINQLQIGQDPSEVKGVMTHAHRGGEGFTPRG
                     LARDVNSFPAPARHLTKKLRHKYHMAFDGPMAALESSRGCPFDCNFCSVWVFFDRKAR
                     VKAPEKALEEIAGIEEKEIFLTDDIAFVNRKESEGLALLLKQSGIKKWYTCETRADLI
                     VRNKDLLKLWKEVGLKTVFVGIEKIDDEGLKSVNKRMKATTNEKALEVLTSIGIRPIA
                     TFIVDPMFTEEQFDRLKEYAIVNNLIAPTYTILTPLPGTEVYDERKHELTTKNYLMYD
                     LLHTVLPTRLPLERFYEKFAELYHSGHSTTKIGPRYFIRILKNLKRKNVGIAIKVYQL
                     MQMMRDPNLYLEAHKYLEKN"
     gene            4907..6328
                     /locus_tag="VI935_01305"
     CDS             4907..6328
                     /locus_tag="VI935_01305"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLE24269.1"
                     /translation="MRVLLIQPHQGTNLGLSRVLITEPLGLENIAAPLLEDFHDVRIL
                     DLRIESLKKLSEEIRYFAPQAAGISCSFTTDVYPTLDIAKTIKELTPGTFVFVGGHHA
                     SLLPSDLLEGQVDAIVIGEGEITTKEMINELERGGDVNKVKGVMTHEGFIPREIIKNL
                     DDMPLPARNLTKSLRRHYHIGFDCPMASLETSRGCPYECNFCSVWVFYSRKIRGKSPE
                     KLAEEIANVKEKYIFLTDDIAFLHKDPAVQLALKLKEMGVKKRFTCETRADIVVRHKD
                     VIELWKEVGLDFIFLGLEKIDDDGLKSVKKHTKASVNEKALNLVKSLGIRPMATFIVD
                     PEWEEEDFEKLKNYVIKHKLSTPSFTILTPLPGTEIYDERKNDLVTLNYQMFDLLHVV
                     LPTKLPLREFYARFADLCRQGHKNTKLGWEFVNKVTMGMIHGNPWAAVRIFNVARMMR
                     NPETYLDAHKYLEETPPILSLQS"
BASE COUNT         2007 a         1420 c         1224 g         1856 t
ORIGIN      
        1 caccattcaa tccgtactga aaagagctac gtgacctgta tcaaaaatta taccatttct
       61 aaaaacaaat tgtgcattta ttttctcctt tcttaacttc ttattatgaa gaagattgta
      121 ggggctgatg ccctcatcag ccctaactag gctcgttcag ggaactagcc ctacattttg
      181 aaatgcacta taagttaata gaattggcgt tacacctttt ttcacaataa acgccatcct
      241 aaggagctgg gacgtgctga aattgaggtt ttctaaccca tctgaggatt tactgtggat
      301 tcgggtaatc ctaaggcttc cttaccttta acatctctga tctctacgtg aacctcgtca
      361 ccaatcttaa aatcaccatc tagaatacgc acacttatat aattcggagt agttccccta
      421 gaccctcctt cgactgaccc ttcactgcgt tcagggcttc ggcttaggac aggtctttcg
      481 acaagaacag taaatcttct tcctatgaaa cttcggtaga attcagtttt tttcttttga
      541 cctaactccc tgagaaggct gcttctgtct tttatcacct gagggtgtac ctgatttcgc
      601 atgatagctg caggggtgcc ctttcttttt gagtatggaa agacatgaaa ataggtaaga
      661 gttgattctt tagctaaatt gaaactgttc tcgaattctt catccgtctc tccgggaaag
      721 ccgaccataa tatcagttcc gattgccgca tccggcatct tttttctaat aaggttagta
      781 caatgaagaa acttatctgg cgtatatctc cttctcattc ttttcagtac atctccatcc
      841 ccactttgaa gtgcgacgtg aaactgtgga cagatggtcc ctgaacttgc aacgaaatta
      901 atgagttcat ccgccgtatc tgcagggtca agtgaactta gtcgtaccct ttttatgatt
      961 tttgcctttt ctatttcttc aaggagtttt aaaagcttcg tcccaatatc ccttccataa
     1021 ctcgcaagat gaaccccagt aagaaccact tctttgtatc ctgattgggc aagcacctcc
     1081 attcttctca agacttctat cacaggaaga cttcttgacc taccccttgc ataggggatt
     1141 atgcaaaacg tgcatctata attacagcca tcttggactt taaggaatgc tctagtacgt
     1201 tccggaaagt ttttaatctc aggtgattca aattccctta ccttgaaaat atcagaaaca
     1261 ataaccctag gctgaggctg tggttttgca tcctgaatgg cttcttttaa agagaacttg
     1321 tcaaagttgc ctactatata atcaactccg tttaccttct caagctcact cggagaaacc
     1381 tgagcgtagc accctgtaac aataacaact ccatccggat ttattctctt tgcacgactt
     1441 atgtagtgtc ttgcctctgc atctgccttc tgagtaaccg tgcaggtgtt tatcacatat
     1501 atatcagcct cctcaggaaa cgaaacctca ctgtatctcc cctcgggaag cgtatttaaa
     1561 atagctgcac tgtcatactg attgaccttg caccctagcg taacaaacga gaccctcttc
     1621 actacaatac ctccttaaat tgcgtaagga gaattcatga attgcaccta cattacatcc
     1681 acgctttact agagaacctc cttaatccat cctccaccta aaaccctatt accctcgtaa
     1741 aaaaccacag cctggccagg cgttatcgca cgctggggat ctttaaattc cactaaagcc
     1801 tctccatctg atttcatacg aactacggaa tcactctctc tatgacggtg tcgtatcctg
     1861 gtctttactc gaatctcatc cgatgggctc tcattaatcc aaattacatc acttgccata
     1921 agcgtagttc tagaaatgtc attctcttct ccaactacaa ccttatttga ttcaagatca
     1981 accttaataa cataaagcgg ctttcctttc gaaatcccaa gccctcttct ctgacctact
     2041 gtgaatgaga aaaccccgtc atggttgccg aggacattcc cattaatatc aacaatctcg
     2101 ccctttccgt tttttacctc agcatgagct ttaagaaact ctctataatc cccgcctgtg
     2161 ataaagcata cctcctgact gtcaggctta ttcgccacct ttatatccat gctttttgca
     2221 atatttctaa cctcactctt cgtcatcgaa ccgagtggaa agataagacg cttaagttcg
     2281 ttctgagtaa gggcaaaaag aaagtaagac tgatccttac tctcatcatt tgctttacag
     2341 agataaaacc tctttccttc ctcatcagtt tctactcttg cataatgacc cgtggcaaga
     2401 tagtcagcgc ctagttccat agcgcgcctt agaagaaaat tgaacttcat aaactgattg
     2461 cagagcacac aagggttcgg tgttcttcca gaaaggtatt tattgataaa atcctccacg
     2521 atatacttct tgaaaacatc catatagttc actacgtaat gtggtatacc gatctcttcc
     2581 gcaaccattc tcgcatctct cacatcgtca gcggagcaac agccgccttc tccgtctccg
     2641 tagtcccaaa gctgcatagt cacaccaata acttcgtgcc cttcctcttt gagaatagca
     2701 gccgttgtag aactatctac ccctccactc attgctacaa ggattctctt cttcatattg
     2761 aattataaga ttacctgatg cttaagcttg ctcaagtaag tggctttcta tgatatttaa
     2821 atttttgtag aggagttgct ttgtcacctt ttaagttcat aagcttgaca atagactaac
     2881 taaaactatt cttttagtga ttggtagggg ttacttcatt aagtagaatt tgtataattc
     2941 ctagattcgg aggaaataaa atgtcttaca aagtcagtgt ggttattgaa aaagatgaat
     3001 acggatatta cgcatactgc cccgagcttg aagggtgcca aacacaaggg ggttctctcg
     3061 atgaagttat gagcaatata aaagaggcta cagaactcta cttagagaca ttatcagaag
     3121 aagaaagaaa agagctactc agcaaagaga ttttaaccac tgccttagag gtaaaagttg
     3181 cctaagttac cacggttgac ttctaaggag gcagaagaaa tacttctcaa agatggtttc
     3241 aaattaatta gaagtaaagg aagtcacagg atatatatga aaggaaataa aagaatagtt
     3301 attccatttc atacaggcat tacattacac ccaaagatag ttaagcaagt gctaaagtgt
     3361 aggaagtgaa taacattatc taaaagtaat atatctcccc agcctgaata atttcatttc
     3421 aaataataaa aacccacttg ctttttaaaa atccaaatta tataatcata ttccactatg
     3481 agagtacttc ttattcaacc tcagcaagga acgagattcg ggataagtaa aattgttaca
     3541 actgaacccc tagggcttga gtcaatagcc tctcctatgc tccttgatgg tcacgatgtc
     3601 agaattatag acctaaaggt agaaaagcca tctagattgg acgaagagct taagaagttt
     3661 tcccctgagg tggtcggtat aagctgttcc tttactactg atgtttatcc tactattgag
     3721 gtggcaaatc atataaaggg ttttaataag aagatctttg tttttatagg cggccaccac
     3781 gcctctctcc tcccttccga tctcttagtt ccctcagtag atgcaatcgc aatcggtgaa
     3841 ggagagatta caggcaagga gcttataaac cagctacaaa taggtcaaga cccatctgag
     3901 gtaaaagggg ttatgaccca tgcacaccgg ggaggtgaag gttttacacc aaggggactc
     3961 gcacgagacg taaattcttt tcccgcgcct gcaagacacc tgacaaagaa attaagacat
     4021 aaatatcata tggcttttga tggcccaatg gccgctctag aatccagccg tggttgccct
     4081 tttgactgta atttctgtag tgtgtgggtc ttctttgata gaaaagcccg ggtgaaagct
     4141 ccagagaagg ctttggaaga aatagctggc attgaggaaa aagagatatt tcttaccgat
     4201 gatatagcat tcgttaaccg taaggaatca gaaggactcg ctcttctcct taaacagagc
     4261 ggaatcaaaa aatggtatac ctgcgaaaca agagcagact taatagtgag aaataaagac
     4321 ctgcttaaac tctggaaaga agtggggctt aaaactgtgt ttgtaggaat tgaaaagata
     4381 gatgatgagg gtttaaaatc cgtaaataag cgtatgaagg caacaacaaa tgaaaaagcc
     4441 ctagaggtct taacatccat tggaatcaga cctatcgcta cctttattgt tgacccgatg
     4501 tttacagaag agcaatttga ccgcctaaag gaatatgcca ttgtaaacaa cctaatagcc
     4561 ccaacttaca caatacttac cccccttcct ggcactgagg tttacgatga gaggaagcat
     4621 gagctaacta caaaaaatta tcttatgtat gacctactac acaccgttct tcccacaagg
     4681 cttccacttg agcgtttcta tgagaagttt gctgaacttt atcactccgg acactcaaca
     4741 acgaaaatcg gaccaagata ttttataaga atccttaaga acttaaagcg aaagaacgta
     4801 gggatagcta ttaaggttta tcaactaatg cagatgatga gagacccaaa tctttatctg
     4861 gaagctcata aataccttga aaaaaactaa ttaataggag gttgccatga gagtattact
     4921 cattcagccc catcaaggta caaaccttgg gttgagtagg gttttaataa cagaacccct
     4981 cggactcgaa aacatagctg cccccctcct tgaagatttc cacgatgtaa gaatactgga
     5041 cctaagaatt gagagcctaa aaaagctctc tgaagagatc cggtacttcg ctcctcaagc
     5101 tgcgggaatt agttgttcat tcacaacaga tgtataccct acattagata tagccaaaac
     5161 tataaaagaa cttaccccag gcacatttgt attcgtaggg ggacaccacg catcactact
     5221 tccatccgat cttttagaag gtcaggtgga cgccattgta ataggagaag gggagattac
     5281 aactaaagaa atgatcaacg aattggaacg cggaggggat gtaaataaag tcaaaggtgt
     5341 tatgacgcat gagggtttta taccaaggga aatcataaaa aacctggatg atatgccact
     5401 tcctgcgagg aatcttacta agagtctaag aagacactat cacataggat ttgactgtcc
     5461 aatggcatct cttgagacga gtagaggatg cccatatgaa tgtaactttt gcagcgtttg
     5521 ggtcttctat tcaagaaaga taagaggaaa atcgcctgaa aaactagccg aagaaatagc
     5581 taatgtaaaa gaaaagtaca ttttcctcac agacgatatt gcatttctcc acaaagaccc
     5641 tgctgtacaa cttgctctta aactcaagga gatgggggtg aaaaagagat ttacatgtga
     5701 aacaagagca gatattgttg ttcgccataa agatgtgata gagctttgga aagaagtcgg
     5761 cctcgatttt atcttcctag gtcttgagaa gatagatgat gatggactaa aatcagtaaa
     5821 gaagcataca aaagcaagcg tcaatgaaaa ggcactcaac ttagtaaagt cactcggaat
     5881 aagacctatg gctacgttca tagttgatcc tgaatgggaa gaagaagact ttgagaagct
     5941 aaagaattat gtaattaagc ataaactatc tacaccttcg ttcacaatcc ttacccctct
     6001 tccaggaacc gagatttacg atgaaagaaa gaatgatctt gttacgctta actatcaaat
     6061 gttcgatctt cttcatgtcg ttcttcctac aaaacttcct cttcgtgaat tctatgcaag
     6121 attcgctgac ctctgtcgtc agggacataa aaacacaaaa ctaggctggg aatttgttaa
     6181 taaggttaca atgggaatga tacatggaaa cccctgggcc gctgttagaa tatttaacgt
     6241 tgctagaatg atgagaaatc ccgaaactta tctcgacgcc cataaatacc tagaagaaac
     6301 tccgccgatc cttagccttc agtcgtgaac aacgaatgtt gcctagattt tttagacact
     6361 tgtgggagag attaaaagag gaggtccact tcgactttat aactttggtt gtatgtattc
     6421 tcccaattgc atccttagat ttaattctac aggttcgagg tagaataatt caagccactg
     6481 acttagatta caattctttg gttcgga
//
LOCUS       DATDRL010000033         2841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1794633, whole genome shotgun sequence.
ACCESSION   DATDRL010000033 DATDRL010000000
VERSION     DATDRL010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794633"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VI935_01310"
     CDS             <1..1185
                     /locus_tag="VI935_01310"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HLE24270.1"
                     /translation="SIDEIDIKEKRVFIRVDFNTPLNKDDKVADDTRILAALPTIEYA
                     LREKARIIIASHLGRPGGKIHPKLTLDPVGRKLSEILRCEIIFPKDCIGDAVRKLSGD
                     LSPGGVMLLENLRFHKEEEENDPRFAKRLAEIADIYVNEAFSVSHRLHASVVGILDYI
                     ETACIGLRFKKEMENLNRLIENPEHPFVAIFGGRSASEKIQIMESLLDKVDIILIGGA
                     ISNTFLKALGTEVERSLVDQIALYSAAKLISSASVRDVRVVLSEDAVLIKGDLNNYSN
                     SFVSSTGAFPKDATSVDIGPKTINDFASRISSAKTILWNGPLGVYEAEDFRKGTMEIA
                     KAIAQSKAFSVVAGWDTVVSIKKGIFTNGNIYLSTGGKATLEFIQGKNLPGLEALTKR
                     LR"
     gene            1182..1952
                     /gene="tpiA"
                     /locus_tag="VI935_01315"
     CDS             1182..1952
                     /gene="tpiA"
                     /locus_tag="VI935_01315"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HLE24271.1"
                     /translation="MRKKLIVGNWKMNMLRKEALELAQELSELLGNLNEVDIVIAPPY
                     TSLDIVYQVIRKTNIKLAAQNVSWEPSGAFTGEVSPYMLRDSGCRWVIIGHSERRGIL
                     GESDTMVQRKIKASIKTGLIPILCVGETLEERERGNTMKVIKNQIENGLNDVNINNPD
                     SIAIAYEPVWAIGTGKNATPEEAEEVHVLIREILSKIFIGIADKMRILYGGSVTPENI
                     KDLISPPNIDGALVGGASLRANSFAKIVKIIGGVQNWT"
     gene            1943..2389
                     /gene="secG"
                     /locus_tag="VI935_01320"
     CDS             1943..2389
                     /gene="secG"
                     /locus_tag="VI935_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HLE24272.1"
                     /translation="MDIIITSVTIFHIIISILLIITVLLQPGKGDDLGTIFGGTSQSI
                     FGASGAVPFLTKVTRILAVLFIITSLSLGYFSAKSIKSSVVNESRPLTKEEGMVIPPE
                     GQSPTKPDKESTINPESQQRTTQSQGNELSSPESEPQPQEVPSSNK"
     gene            2419..>2841
                     /locus_tag="VI935_01325"
     CDS             2419..>2841
                     /locus_tag="VI935_01325"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE24273.1"
                     /translation="MGILDSVFSKKKEEKENEPAKTKLVEDADSLLKSGVRFYNDGYY
                     EKAESELRRVLNLHPKHVEAHYYLGKIHESKIHGEDDFETMEEAMNEHQEAIKIDPAY
                     LNAHISLGRLYVRSGFYEGAIRELEEAVRINPNSPEAHA"
BASE COUNT          951 a          511 c          647 g          732 t
ORIGIN      
        1 tccattgacg aaatagatat aaaggaaaaa agggttttta tcagggtgga tttcaacaca
       61 ccactcaata aagacgataa ggtcgcggat gatacgagaa tccttgcagc actgcccact
      121 atagaatatg cacttaggga aaaagcgaga ataataattg catctcacct aggaaggcct
      181 ggcggaaaaa tacacccaaa acttacgctt gatccggtag gaagaaaact ctcagaaatt
      241 ctaagatgtg aaataatctt tcccaaagac tgtattggag atgcagttag gaaattatcc
      301 ggtgaccttt ctcctggagg cgttatgctt cttgagaatc tcaggttcca taaagaagag
      361 gaagaaaacg atcctaggtt tgcaaagaga cttgcagaaa ttgccgatat atatgtgaac
      421 gaggcattct cagtatctca tagattacat gcttcagttg tcggaatatt ggattacatc
      481 gagaccgctt gtatcgggct aaggttcaaa aaggagatgg aaaatttaaa ccgattgatt
      541 gagaatcctg aacatccatt tgtggcgatt tttggtggtc gtagcgcttc agagaagatt
      601 caaataatgg aaagcctatt ggataaagtt gatataatat taatcggggg agcaatctca
      661 aatacctttc tcaaagcctt aggaacggaa gtagaaaggt cactagtgga tcagatcgct
      721 ctatatagtg ctgcaaaact aatttcctca gcaagcgtgc gcgatgttcg agtcgttctt
      781 tctgaagacg ctgtacttat aaaaggtgat ttgaataact actctaattc ctttgtaagc
      841 tctactggag cgttcccaaa ggacgctact tcagttgata taggaccaaa aacaatcaac
      901 gattttgcct cgaggatatc aagtgcaaaa accattttat ggaacggacc gttaggtgtt
      961 tacgaggctg aggactttag aaaagggacc atggaaatag ctaaagccat agctcaatct
     1021 aaagccttta gtgtcgttgc aggctgggat acagtcgtat ctattaaaaa agggattttt
     1081 actaatggta atatttatct ttccactggt gggaaggcaa ccctagaatt cattcaagga
     1141 aaaaacctac caggacttga agcactaacg aaaaggctaa gatgagaaaa aaattgattg
     1201 ttggaaactg gaagatgaat atgctaagga aagaggcgtt agagcttgca caagagctat
     1261 ctgaactcct cggaaattta aacgaggtgg acattgtgat agcaccgccc tatacctcgc
     1321 ttgatatcgt atatcaagtt attcgtaaga ctaatataaa gctcgcagct caaaatgttt
     1381 cctgggagcc atctggagca tttacaggag aggtatctcc gtatatgctt agagattctg
     1441 gatgtagatg ggtaattatt ggtcattcgg agaggagagg catattaggc gagagcgata
     1501 ctatggtgca gagaaaaatc aaggcctcaa ttaaaacagg gcttattcct atactctgcg
     1561 ttggtgaaac ccttgaggaa agagaaagag gaaatacgat gaaggtgata aaaaaccaga
     1621 ttgaaaatgg actcaacgac gttaatatca acaaccctga tagtattgca atcgcatatg
     1681 aaccggtttg ggcaattgga acaggaaaaa acgctacacc cgaagaggct gaggaggttc
     1741 atgtccttat tcgagaaata ttgagcaaga tttttattgg tattgctgat aaaatgagga
     1801 ttctctacgg tggtagcgta acgcctgaaa atataaagga tttaatttca ccaccaaaca
     1861 tagacggagc attagttggc ggagctagcc ttagggccaa ctcttttgca aaaatagtga
     1921 agataatagg gggtgtccaa aattggacat aatcataacg tctgtaacaa tttttcacat
     1981 aattataagt attctactta taatcaccgt gcttcttcaa ccaggtaaag gggacgatct
     2041 tggtacgatt tttggaggta cgagtcaatc aatctttggg gcgagcggtg cggttccatt
     2101 tcttacgaag gtcactagga ttcttgcagt gctcttcata attacgtcgc tatccctggg
     2161 atatttctca gcaaaaagca taaagtcctc agtggtaaat gaatcaagac cattaactaa
     2221 agaagagggg atggtgattc caccagaagg tcaatcccca actaaacctg acaaagagtc
     2281 tactattaat ccagaatccc aacaacgaac cactcaatct cagggtaacg aactatcgtc
     2341 gcctgaatca gaaccacaac ctcaagaagt accctctagt aataaatgag tattttttta
     2401 acctaggagg ttgccggaat gggcatactt gactcggtat tcagtaagaa aaaagaggaa
     2461 aaagaaaatg agccagcaaa aacaaagcta gttgaagatg ccgattcttt attgaaatca
     2521 ggggtccggt tttacaacga tggatattat gagaaagcag agtctgaact tagacgggtt
     2581 ttgaatctac accctaagca cgtagaggca cattattatc ttggaaagat tcatgaatcc
     2641 aaaatccatg gcgaagacga ttttgaaaca atggaagagg ctatgaatga gcatcaagaa
     2701 gccataaaga tagatcctgc ttatctcaat gcccacatta gtttggggag gctatatgta
     2761 agaagcggtt tttatgaggg ggcaattaga gagctagaag aggccgttag gataaatcca
     2821 aattcaccag aggctcacgc c
//
LOCUS       DATDRL010000034         6764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3637174, whole genome shotgun sequence.
ACCESSION   DATDRL010000034 DATDRL010000000
VERSION     DATDRL010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3637174"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..103)
                     /locus_tag="VI935_01330"
     CDS             complement(<1..103)
                     /locus_tag="VI935_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HLE24274.1"
                     /translation="MRKGKVKWFNESKGFGFIEQEGGKDVFVHYSAIQ"
     gene            371..889
                     /gene="tpx"
                     /locus_tag="VI935_01335"
     CDS             371..889
                     /gene="tpx"
                     /locus_tag="VI935_01335"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HLE24275.1"
                     /translation="MAQERAGAITMRGNPVTLVGSEIKVGDKAPNFTSLEALGSPITL
                     ESLKGKVKVFNVIVSVDTPVCDAQTRRFNEEAAKLPGDVAILTLSMDLPFAQKRYCGA
                     AGIDKVKTASDYRDASFGQAYGILIKEHRLLGRAIFVVDKNDTVQYVEYVKEVTQHPD
                     YDAALAVVKKLA"
     gene            1004..1561
                     /locus_tag="VI935_01340"
     CDS             1004..1561
                     /locus_tag="VI935_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HLE24276.1"
                     /translation="MGFGEMLSKIFKNESETKAPEKTGQDSKAETSTTERFVTRNENT
                     IIEEKVGSASQITESGQMEEIQLEEDLETEGVKTQESATSNEEVTKEKILEALSDVYD
                     PEIPIDIVNLGLIYDVQIENGNVFVKMTMTAPGCPSSAQIAAESQMLIEGLRGVKEAS
                     IEIVWDPPWDPSRMSEEAKKGLGFM"
     gene            1749..2234
                     /locus_tag="VI935_01345"
     CDS             1749..2234
                     /locus_tag="VI935_01345"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HLE24277.1"
                     /translation="MAKMPKVGDKAPDFEAETYGGKKIKLSDFTNKKTVALYFYPRDN
                     TPGCTKEACSMRDGMKVLEKLGIQVLGVSTDSVKSHEGFRDKYNLNFPLLSDKSKKLV
                     TTYGVESDYGSARRVTFLIDKTGTIRHIWEKVNTAGHSDEVINKIKELRLSTIRHGLT
                     L"
     gene            2371..3111
                     /gene="bioD"
                     /locus_tag="VI935_01350"
     CDS             2371..3111
                     /gene="bioD"
                     /locus_tag="VI935_01350"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HLE24278.1"
                     /translation="MSYLLKGIFITGTDTAVGKTVVSAALALTLKQSGNKVAVMKPVQ
                     TGTDLGVCLDIEFVHTVIEASYSLDEVCPYRFPYPLAPLVAANLAGEMIDLERIKSCF
                     NKLSSIHDTVIVEGAGGLLVPITDGYLMSDLAFDLGLSLIIVTGPGLGTINHTLLTVE
                     SAKARGLKVMGIVINQFPSNPGLAERTNPELIHRMIGAPILGVLPFDSGICVEEGRAG
                     RLREIASSSFTPELGGSFEVEGFLSKMK"
     gene            3679..4251
                     /locus_tag="VI935_01355"
     CDS             3679..4251
                     /locus_tag="VI935_01355"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HLE24279.1"
                     /translation="MMKKLLKLCSGVSLLILIFLGFYIYTAESSTKAPEQPIFFSHKI
                     HAGDNQIPCQYCHSYVTESTMPGIPSMQKCMGCHTFIAGKDEEYKYDGKTINMQKEIQ
                     KVKEYWDKKEPISWIRVNYLPDFVYFTHKRHVLRKGIECKNCHGEVEKMDVVQRVHRL
                     QMGWCVSCHQANAKDGDELTKLKDCLTCHK"
     gene            4264..>6764
                     /locus_tag="VI935_01360"
     CDS             4264..>6764
                     /locus_tag="VI935_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLE24280.1"
                     /translation="MSEIKKGGSAPRNSSRVNTGGIKRRDFLKILGVAGGTVAITGGC
                     SPEPVEKIIPYVIPQDTIIPGIPKWYASTCMECPAGCGILVKNREGRAIKIEGNPLSP
                     INSGGTCARGQAALQGLYNPDRIRGPLTRNSSGKLEPTSWDEAEGVLVEKIREIRGKG
                     NGNKIVFLTNNISGSLNKLIDDWLDALGGGSTGPVLSKGEGVTTGGRHIVYETFSHEP
                     LKEANRIAFGINKIPTYNIEKTKYLLSFGADILETWLSPVEYAKKFSGMHGYKDKSMG
                     KFVQVEPRLSLTGASADEWIAIRPGTEAFLALGIANAILDSGLASITGYETDRLNWLL
                     QDYKPDVVSSITDIPTETITRIVKEFVDLRPSLALGGGAATTSSKSTETLVAINILNY
                     IAGNVGDTVNFGDNLALSNANSFREIASLVDSMSKGEIELLLIYNVNPAFTLPKSLKF
                     DEALKKVPSIVSFSSFIDETTEQAGLVLPDHTTLEKWGDYIPQESVYGLIQPIMDPVF
                     NTKSTGDVILSISKKVEGLESQFASNTFFDYLRDSWRRFHSSYAPDRDFEAFWTETVE
                     RGGIYREVVPIQVQLSQEVSKLSFSEPKFEGDGTIYFMAYPSYRYYDGRGANKPWLQE
                     LPDAITTAVWDTWVEINPETARKMGVKEGDFLSIESPYGNITTQAYIYKGIRPDTIAV
                     PIGLGHKSYGRYAQNRGVNPIEILPVLTDEASGGFAWLSTKVNVSKTGRLEQLVKTQE
                     GTSQDGRGIAQTITLKELEGGMHEEHGEDEKFDMYPPQQYIKHRWGMSIDLSKCIGCG
                     ACVTACYAENNISVVGKAQVAKRREMSWIRIERFY"
BASE COUNT         2069 a         1232 c         1599 g         1864 t
ORIGIN      
        1 tctgtatagc actgtaatga acaaaaacgt ccttaccgcc ttcctgttca atgaatccga
       61 aacctttgga ctcattaaac cactttactt tacctttacg caatttactg cctccttaaa
      121 attaatggct taaatatcac atgcttttat agccttgtca aatcttatct attagacgca
      181 aataggcatg aatgataata ggcaagatgc gggatgcaaa atacaaaata cgtcatgaat
      241 catccatcgt gaatcgtttt attatcttaa attcgtggct aaataatcac cacttcctat
      301 tgacactaaa gggtcttata tatagtctaa tgctatatct aacaatatcc tttgaggagg
      361 tattagaata atggcacagg aaagagccgg tgcaattact atgagaggga acccggtaac
      421 actagtaggt tctgaaatta aagtcggcga taaagcccca aatttcactt ctctcgaggc
      481 cttgggatcg ccaattacac ttgagagttt gaagggtaaa gttaaggttt ttaatgtgat
      541 tgtttcagtt gataccccag tttgcgatgc ccaaacgagg cgttttaacg aggaggcggc
      601 taagcttccg ggtgacgttg caattcttac tttgagtatg gatttaccat ttgctcaaaa
      661 gcgctattgt ggtgctgctg ggattgacaa ggttaaaact gcatctgatt atcgtgatgc
      721 ctcctttggt caagcctatg gcattttgat aaaggaacac cgtcttcttg gcagggctat
      781 atttgtagta gataaaaacg atacggtaca gtacgtggaa tacgttaaag aagtaaccca
      841 acatccggac tatgatgcag ctcttgctgt tgttaagaaa ttagcttagg tgatgattta
      901 ggagagcccc gagttaatgg gattgggctg gatattcttt atatacaaaa cctgttcagg
      961 tattattatt aaagtgataa tatttagcta aggaggtatt agtatgggat ttggtgaaat
     1021 gctatcaaag atttttaaaa acgaaagtga aacaaaagcc ccagaaaaaa ccggacagga
     1081 cagcaaggct gaaacttcaa cgaccgaaag atttgtaact agaaacgaaa atacgatcat
     1141 tgaagaaaag gttggaagcg cttctcagat cactgaatct gggcaaatgg aggagattca
     1201 acttgaagaa gacttagaaa ccgagggagt caagactcaa gagtctgcca cttctaatga
     1261 ggaggttacg aaagaaaaga ttttagaggc gctgagcgac gtttatgatc ctgagattcc
     1321 gatagatatt gtaaaccttg gtcttatcta cgatgttcag attgagaatg gaaacgtttt
     1381 tgtaaaaatg acaatgacag cacctggctg tccttcttca gctcagatag cagccgagtc
     1441 acagatgctt atagaaggac ttcggggggt taaggaagcg tctattgaaa ttgtgtggga
     1501 cccaccctgg gatccaagca gaatgagcga ggaagcaaaa aagggcttag gatttatgtg
     1561 atgaagcaag caataaattc taaattacaa atcacaaatt ccaaataaat ccgaaatctt
     1621 agattcaaat gtttggtact cagagtttag agcctggtgc ttgtttgtga tttggtcatc
     1681 ggctcttgtt tattagcggc tacttaatag cttttccatt tataaccgag gcaaggaggt
     1741 tttcttagat ggcaaagatg cctaaggttg gtgataaggc tcctgatttt gaggctgaga
     1801 catatggagg aaaaaaaata aagcttagtg attttacaaa caaaaagaca gtagctcttt
     1861 atttttatcc aagagataat acaccgggct gtacaaagga ggcgtgttcg atgagggatg
     1921 gcatgaaggt gctagaaaaa ctgggtattc aagtattggg agtaagcact gacagtgtaa
     1981 aatcccatga ggggtttaga gataaataca accttaactt tccccttttg agcgataaaa
     2041 gtaagaagtt agtaactacc tatggtgttg agagtgatta cggctcggca agaagggtaa
     2101 cttttctgat agataaaaca ggtactatta gacatatttg ggaaaaggtt aatacagccg
     2161 ggcattctga cgaagtgatt aacaagataa aagaactccg attatcaacc atccgccacg
     2221 gattgacact atagagacga atacaataag agagatgtac aggatgcaaa atacacgatg
     2281 caagatttat catgcatcct gaatcatgta ttatgaatta ttttatctgt gtaaatccgt
     2341 gttcattagt ggctgaaaac aatttctata atgtcctatc ttctcaaagg aatcttcata
     2401 acaggcacgg atactgccgt aggaaaaacc gttgtatccg ctgcgcttgc cctgacatta
     2461 aaacagtccg gtaataaggt tgctgtaatg aagcccgttc agacgggcac tgatttaggt
     2521 gtatgcctgg acattgagtt tgtgcatact gttattgaag cgagttattc attagatgaa
     2581 gtatgtcctt atcgttttcc ttatccactt gcacctttag ttgcggctaa tcttgcggga
     2641 gaaatgattg atttagagag aataaaatct tgcttcaata agctttcttc gattcacgat
     2701 actgtcattg tcgagggggc tggaggatta cttgtgccca ttactgatgg ttatctaatg
     2761 tccgacttgg catttgattt aggtctatca ttgatcatag ttaccggtcc aggtcttgga
     2821 acgataaacc acacccttct cacggtagaa tctgcaaagg ctagggggct taaggtaatg
     2881 ggaatagtga taaatcaatt cccttctaat cctggtcttg ccgaacgcac aaatccagag
     2941 ttaattcata gaatgatagg agcaccgatt cttggtgttc tcccatttga ctctggaatt
     3001 tgcgtagaag agggaagggc tggaaggctt agagaaatag catcctcttc ttttacaccc
     3061 gagcttggcg ggagttttga agttgagggg tttctatcca aaatgaaata aattgacaaa
     3121 cggtcacgaa caatactcgt gacccaccct gtggtaattt aacaattcgc attttggaaa
     3181 cgtggatagg tgggtttttc taacctcacg agacaagcct gtcctgagtt taacgaagga
     3241 aatgtcttgt ctatcgtcac tacaataaat tgggtccgcc agtctcgcta gcggagggtc
     3301 aacaggcaac gagcaagcct gtcccgagtt taaaccgagg ggactcgttg cctacccatt
     3361 atacggggat ataaaacaaa aacaacgggt aatcaccagt ccctgccccg agcaaagtcg
     3421 aagggttgct ggtgggcata agcccgtcct catcttgatg aaaacctaaa gattaggtgc
     3481 tgagaaatga aagatgctca tagcttttgg gatgaattaa gctcttctgg taagtaattc
     3541 aatggctaga aaacgaaaga cctttagttt acgtattaca acttgacaga ttgtttgagt
     3601 atgatataca tactaagttt tttttgtacc taagttttag ctttccatta ggggtgcaat
     3661 ccaaatttaa ggtagaggat gatgaaaaaa ctcctgaaac tatgctctgg tgtttcactc
     3721 ctaattctaa tctttcttgg attttacatc tacactgcag aaagcagcac taaggcccca
     3781 gagcagccaa tttttttcag ccataagatt catgccggtg acaaccaaat tccctgtcaa
     3841 tactgtcata gctacgtcac agaatcaacg atgcctggaa ttccctctat gcaaaaatgt
     3901 atgggttgcc acacttttat tgctggaaag gatgaggaat acaagtacga cggaaagaca
     3961 ataaacatgc aaaaggaaat tcagaaggta aaagagtact gggataaaaa ggagccgatt
     4021 tcctggatta gagttaatta tctacctgat tttgtttact tcactcataa aaggcatgtt
     4081 cttcgtaaag gaatcgagtg taagaactgt catggtgagg tagaaaaaat ggatgtagtt
     4141 caacgtgtac atagactcca gatgggatgg tgtgtaagtt gccatcaagc taacgcaaag
     4201 gatggggatg agctgactaa gctcaaggat tgtttgacct gtcataaata gaatggagga
     4261 taaatgtcag agattaagaa gggcggttcg gccccgagga attcatctag ggtcaacaca
     4321 ggcgggataa aaagaaggga tttccttaaa atacttgggg tggctggggg cactgtggca
     4381 attacggggg gttgttctcc agagcctgtg gaaaagataa taccctatgt tattcctcag
     4441 gatactatta ttccagggat tccaaagtgg tacgcaagca catgtatgga atgccctgcg
     4501 gggtgtggaa ttctcgttaa gaatcgtgag ggaagagcga taaagatcga agggaatcct
     4561 cttagcccga ttaattctgg aggaacatgc gcccgtggac aggccgcact ccaggggctt
     4621 tataatcctg accgtattcg tggaccgctt acaagaaatt cttccgggaa gcttgaacct
     4681 acgtcatggg atgaggcgga aggtgtactt gttgaaaaga ttagggaaat cagagggaaa
     4741 ggcaacggaa ataagatagt cttcttaacc aataatatct cagggagtct aaataaactc
     4801 atagacgatt ggttggatgc actcggtggt ggttcgactg ggcctgtcct gagcaaaggc
     4861 gaaggggtca ccacaggtgg aagacatatc gtttatgaaa cgttttcaca cgaaccgctt
     4921 aaagaagcaa atcggattgc gttcggaata aataagatcc ctacatataa tatcgagaaa
     4981 acaaaatatc ttctctcatt cggtgcagac atactcgaaa cgtggttatc tccagttgaa
     5041 tacgcaaaaa agtttagcgg tatgcatggc tacaaagata aaagtatggg taagtttgtt
     5101 caggtggagc cgaggctatc tcttacgggt gcaagcgctg atgagtggat agctataaga
     5161 cctggaactg aggcattcct cgcactcgga atagcaaacg ccattcttga tagcggctta
     5221 gcctctatca cgggttatga gacagatagg ttaaactggc ttcttcaaga ttacaaaccc
     5281 gatgttgttt cctcaattac agatattcca acagaaacca taactcgtat tgtaaaggag
     5341 tttgtagatt taaggcctag tctggcttta ggcggtggcg cagcgactac ctcgtccaag
     5401 agcacagaaa cgcttgtcgc cataaatatt cttaactaca tagctggaaa cgtaggagat
     5461 acagtaaatt ttggagataa tcttgccctt tcaaatgcaa attcatttcg agaaattgcc
     5521 tctctcgttg actcgatgtc taagggtgag attgaacttc ttctgattta taacgtgaac
     5581 ccagccttta ctttacctaa gagtcttaag tttgatgagg ctcttaaaaa ggttccttcc
     5641 attgtaagct tttcaagctt tattgatgaa acgaccgagc aagcgggcct tgtccttcct
     5701 gaccatacaa ctcttgaaaa atggggtgat tacataccac aagagagtgt ttatgggttg
     5761 attcaaccta taatggaccc agtttttaac acaaagtcta caggtgatgt gattctttct
     5821 attagtaaga aggtcgaggg gcttgaaagc caatttgctt ctaatacatt ctttgattat
     5881 ttaagggatt cttggagacg atttcattca agttatgctc ctgataggga ttttgaagcc
     5941 ttttggacag agacagtgga gcgaggaggt atttatagag aggttgttcc tatacaagtt
     6001 caactttctc aagaagtgtc gaaactatca ttctcagagc ccaagttcga aggcgacggt
     6061 acgatctatt ttatggctta tccttcatat aggtactatg atggaagggg ggcaaataag
     6121 ccttggcttc aagagcttcc agatgctata acaactgctg tctgggatac ctgggtggag
     6181 ataaatcctg agactgccag aaaaatgggg gtcaaggaag gcgattttct ctcaattgag
     6241 tccccatacg gtaatatcac aactcaggct tatatatata aaggtattag gcccgataca
     6301 atcgcagttc caattgggct tggacataag agttacggga ggtatgcgca aaatagggga
     6361 gtaaacccaa tcgaaattct ccctgtctta actgacgagg cttcgggggg ctttgcatgg
     6421 ctatcaacta aggtaaatgt ctcgaagaca ggaaggcttg agcagttagt taaaacacag
     6481 gaaggcacga gtcaggatgg cagaggaatt gctcagacta taaccttaaa ggagcttgag
     6541 ggtgggatgc atgaggagca tggcgaagat gaaaaatttg acatgtatcc gcctcaacag
     6601 tacatcaagc accgctgggg gatgtcaatt gacctttcaa aatgtatagg atgcggtgcg
     6661 tgcgttacgg cgtgctatgc ggagaataat atttctgtag tgggcaaagc ccaggtcgca
     6721 aaaagacgag agatgtcatg gataaggatt gagagatttt atca
//
LOCUS       DATDRL010000035         5238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1000487, whole genome shotgun sequence.
ACCESSION   DATDRL010000035 DATDRL010000000
VERSION     DATDRL010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000487"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /gene="nosZ"
                     /locus_tag="VI935_01365"
     CDS             complement(<1..1577)
                     /gene="nosZ"
                     /locus_tag="VI935_01365"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR04246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HLE24281.1"
                     /translation="MKQINYGKIMRFLSLPLLVGFIGLLVACKGGQSPAEKVYVAPGK
                     LDTYYAFLSGGHSGQVGVFGLPSGRLIRMIPVFAPDVAVGWGIDEGSKEIMGGYIWGD
                     AHHPALSETNGDYDGRWLFINDTANGRIARIDLKDFVTKEIISVPNVGGNHASPFVTP
                     NTEYVVGATRFSIPVPNRHVPIETYKENFNGVIAFVNVDKDTGKMENAFQILMPPFDF
                     DLADAGKNASYGWAFFSSYNTEEAYKDLEVEASQKDRDFIAAVNWKAAEEAIKEGKYK
                     EINGVKVIDPQEVKGIVYFLPTPKSPHGSDVTPDGKYIVGGGKLAAVAVVHGFDKMQE
                     AIKNGDFDGEVMGIPILKYESVRVAEVPVGLGPLHTQFDDQGYAYTSLFVDSAIAKWK
                     LGTWEVVDKIPVYYNIGHLAAVHGDTAKPRGKFVLAMNKIAKGRYLPVGPSHPESAQL
                     IDITGKKMEMLLDFPTPVEPHYGQIIEATLIKPELIYPLDQNKDPYAIKSADQARVER
                     NGNRVDVYMLAIRSHFTP"
     gene            complement(1614..2087)
                     /locus_tag="VI935_01370"
     CDS             complement(1614..2087)
                     /locus_tag="VI935_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLE24282.1"
                     /translation="MKRKISTIILLGFIAMYVSCKGDKEEKSESEVVETKKEVIQAEQ
                     EKPKAPPPISEELADKGEELFKTKGCIACHTIGKGRLTGPDLAGVTERRKIDWIENQI
                     LHPEVMVEKDPIAKELLATYLVKMPNQNVTPEEAQAIIMYLREEDSEAKKESEHE"
     gene            complement(2276..3382)
                     /locus_tag="VI935_01375"
     CDS             complement(2276..3382)
                     /locus_tag="VI935_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24283.1"
                     /translation="MERKKPKFRFPIMALGGLALILALWGGLFRLGWNLPVVTVNLPY
                     SHGPLMVSGFLGTLISIELAVALKYKWAYVVPILSGIGALALIVGVPSLFGKMLMTLS
                     STGLVFLLVVILYPHPSLHAIIMMLGAFCWLVGNGLWLLGFPFYIVALSWIGFLMLTI
                     AGERLEISRVLQISKEKKLVFIFVVFVFIIGVLLAIKVFDIGVRVASIGMIALALWLL
                     RNDIARRELHQRGLSRYIALSLILGYVWLGVSGLFGLFFGGVSSGFYYDAILHSVFVG
                     FVFSMIFAHAPIIFPTLMGLPTFLPFQSSFYVHLALLHLSLALRIIGDLAAWPLGRQW
                     GALFNAIAILLFLGNIMYLNRKVKRTITQSSSIK"
     gene            complement(3388..3726)
                     /locus_tag="VI935_01380"
     CDS             complement(3388..3726)
                     /locus_tag="VI935_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLE24284.1"
                     /translation="MNKNYTYFIDLCEEIGEIPTNGIISRTLYSDDQLKVILFGFDQG
                     QELSEHTSTMPAIIHIVRGEARLTLGDYPKEAKAGSWVHMPPQLKHSVYAKTPLVMLL
                     ILVKSIEQKV"
     gene            complement(3754..4254)
                     /locus_tag="VI935_01385"
     CDS             complement(3754..4254)
                     /locus_tag="VI935_01385"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HLE24285.1"
                     /translation="MQFSYNLVVLIHVLATIIWLGGMFFIAIVMVPVLRRLEPPQKRI
                     EVLSATATRFRMVSWIAILVILVTGVLNAINRGVTLQAISTSEFFSSYFGKILTLKLI
                     LVFAILVLSAIHDFILGPRLIELLSSSGPDSFQKLQKNRRYVSWLARINALFGILVVA
                     CAVMLS"
     gene            complement(4286..4645)
                     /locus_tag="VI935_01390"
     CDS             complement(4286..4645)
                     /locus_tag="VI935_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009069910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HLE24286.1"
                     /translation="MIKTERVYDEVGKDDGIRVLVDRLWPRGIKKEEVKIDLWLKEVA
                     PSTKLRKWFSHDPSRWKEFCKRYKTELRKDEEKKIALIELTKIATSEKLTLIYSAKDK
                     EHNNAVVLKKLLESLLK"
     gene            complement(4719..5147)
                     /locus_tag="VI935_01395"
     CDS             complement(4719..5147)
                     /locus_tag="VI935_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HLE24287.1"
                     /translation="MFKLSRRYIKTAFAFFIVGLLLGLYMIVSKYILGKWVSPQLITA
                     HAHALLFGFVISLIMGVAIWMFPRPRDEMHYSPGLAEITYWFLTLGTVVRFICEITSS
                     YINSRILDWLIVLGSTSQVLAGLLFIYNVWSRVRPVGKQT"
BASE COUNT         1561 a         1189 c          916 g         1572 t
ORIGIN      
        1 ggagtgaagt ggctccttat cgcaagcatg tagacatcta ccctatttcc gtttcgttct
       61 acgcgagctt ggtcagcact tttaattgcg tatgggtctt tgttttggtc tagtggatat
      121 ataagttctg gctttatgag agtagcctca attatctgac cataatgtgg ttctactgga
      181 gtggggaaat caagtagcat ctccatcttt ttgcctgtta tatcaataag ctgagcgctt
      241 tctggatgag atggacctac agggagatac ctgcctttag cgattttgtt cattgctaga
      301 acaaacttcc ctctcggttt agctgtatcc ccatgaacag cggcaagatg cccgatattg
      361 taataaacag gaatcttatc taccacttcc caagttccaa gcttccattt tgcaattgca
      421 gagtccacaa aaagggatgt gtaagcataa ccttgatcat cgaactgagt atgaagagga
      481 ccaaggccaa ctggtacctc ggctactctc acagactcat atttaaggat aggtattccc
      541 attacctcac catcaaaatc gccatttttg atagcttcct gcattttgtc aaaaccgtgt
      601 acaactgcca ctgctgcgag ctttccgcca ccaactatat attttccatc aggagtgaca
      661 tcggaaccgt gtgggctctt aggtgtaggt agaaagtaga cgataccttt cacctcttgc
      721 ggatcaatga ccttaactcc gttaatctcc ttgtattttc cttccttaat agcttcttca
      781 gcagctttcc agtttacggc ggctataaaa tctctatctt tttgagaggc ttcgacctct
      841 aaatccttat atgcctcctc tgtgttgtaa gagctaaaaa acgcccaacc gtaactcgca
      901 ttcttaccag catctgctaa gtcgaaatca aacggcggca tcaggatttg aaatgcgttt
      961 tccattttac cagtatcttt atctacattt acaaatgcga ttacgccgtt aaaattttct
     1021 ttgtaggtct caattggaac atgacggttt ggcacaggaa tggaaaacct agttgcgcca
     1081 accacgtatt ctgtattggg tgtgacgaat gggcttgcat gattaccccc tacgtttgga
     1141 acgctgatta tctcctttgt aacgaaatcc ttcaggtcta ttcgagctat acgaccattg
     1201 gcagtatcat tgataaataa ccatctacca tcgtaatcac cgttagtttc tgaaagggcc
     1261 ggatgatggg catcacccca aatgtagcct cccattatct ccttacttcc ttcgtctata
     1321 ccccagccta cagctacgtc tggtgcaaaa acaggtatca tccttataag ccttccggaa
     1381 ggaagtccaa agaccccgac ctgacctgaa tgaccaccag agagaaaggc gtaataagta
     1441 tcaagctttc ccggtgccac ataaaccttc tctgccgggc tttgccctcc tttgcaggca
     1501 acaagaagcc caataaaacc aactaaaaga gggagagata agaatctcat tatcttccca
     1561 taatttattt gtttcatttt aattacctcc ttttttgtat aagtttattt ttatcactca
     1621 tgctcagatt ctttctttgc ttctgaatcc tcctctctta aatacattat gattgcctgt
     1681 gcttcttccg gtgtaacatt ctgattaggc atctttacaa gataagttgc cagcagttcc
     1741 ttagctatag ggtctttttc taccattacc tcaggatgaa gaatctggtt ttcaatccaa
     1801 tcgattttcc gtctttctgt aacaccggct agatccggac ctgtgagtct tccttttcca
     1861 atcgtatgac atgctatgca gccttttgtc ttaaataact cttcaccttt gtctgcaagc
     1921 tcttcgctaa tcggaggagg tgcttttggt ttctcctgct ctgcctgaat tacttctttc
     1981 tttgtctcaa caacttctga ttccgatttt tcttctttat ctcctttgca cgatacatac
     2041 attgcgataa aacccaatag gattatcgtt gagatttttc ttttcaattt tcttgcctcc
     2101 ttttggcttg ttttattaaa gcaacctctg tgccaattag aataccttgt aattattgtg
     2161 cattgaaaaa tttgaagtgt gtaagatgtc agaaaatcgg acagcctctt taactttatg
     2221 ctggcgttaa tatccatctg caacttcttg ttttaaattc gaacaatcca aaatatcatt
     2281 tgatggaact agattgagta attgtacgtt ttactttcct attcaagtac ataatatttc
     2341 cgagaaaaag aagaatcgct atagcgttaa ataaagctcc ccactgacga cctagaggcc
     2401 acgctgcaag gtctcctatt atacgcaagg ctaatgaaag gtgaagcaac gccagatgta
     2461 cataaaatga tgattggaaa gggagaaacg ttggtaaacc cattaacgtt gggaaaatga
     2521 tgggtgcatg ggcaaatatc atagaaaaga caaaaccaac aaagacagaa tgtaaaatag
     2581 cgtcataata aaaccctgat gatacacctc cgaagaacaa cccaaacaag ccacttacac
     2641 cgagccatac ataacctaat atcaaactca gagctatata ccggcttaac cctcgttgat
     2701 gtagttcacg tcttgcgatg tcatttcgta aaagccacaa ggctagagca atcattccta
     2761 tgcttgctac acgaaccccg atgtcaaaga ctttgattgc caagaggaca ccaattataa
     2821 aaacaaatac aacaaaaata aaaactaact tcttctcttt agatatctga agcactcgac
     2881 taatttctaa tctttctcca gcaattgtta gcattaaaaa tccaatccag gatagagcga
     2941 ctatataaaa agggaaaccc aaaagccaaa gtccattccc aacaagccaa caaaaggcac
     3001 caagcatcat gatgattgca tgaagagaag ggtgaggata aagaataact actaaaagaa
     3061 atactaagcc agtgctactt aaggtcatta gcattttccc aaacaaacta ggcacaccca
     3121 ctattaaagc caaagcacct ataccactta aaattggcac tacatatgcc catttgtatt
     3181 ttaaggctac cgctagctcg atactaataa gtgttcccaa aaaacctgaa accatgagag
     3241 gcccatggga ataaggtaag ttgaccgtaa ccacaggcaa gttccatcca agacgaaaaa
     3301 gtcctcccca tagcgctaga attaaagcaa gtcctccaag agccattata gggaaccgaa
     3361 acttgggttt tttcctttcc atttggccta gactttttgc tctattgatt taactagaat
     3421 aagtagcatt acaagaggtg ttttcgcata gacgctgtgt ttaagctgag gtggcatatg
     3481 aacccatgat cctgctttag cttctttcgg ataatctcct agcgtcagac gagcttctcc
     3541 tctgacgata tgaataatag cgggcatagt agacgtgtgc tctgaaagtt cttgaccttg
     3601 gtcgaaacca aaaagtatga cctttaattg gtcgtcacta tatagggtac gacttataat
     3661 accgttggtg ggaatctctc caatctcttc acacaggtct ataaagtagg tatagttttt
     3721 attcattgcc tcacctcatc aactattcat cttttaacta agcattactg cacaagcaac
     3781 taccagaatc ccaaagagtg cgttaatcct tgcaagccat gatacgtacc ttcggttctt
     3841 ctgtaatttc tgaaaggaat ctgggccgct actacttaga agctcaatca acctaggtcc
     3901 taaaataaaa tcatggatgg cacttaaaac tagaatagca aacacaagta ttagttttaa
     3961 tgtgagtatc ttcccaaagt aagatgaaaa aaattcacta gttgagatcg cctgtagggt
     4021 aacacctcgg tttattgcgt taagcacacc ggttactaag atgactaata tggctatcca
     4081 acttaccatt cgaaatcttg tggcagtagc tgaaagaacc tcgatgcgtt tttgaggtgg
     4141 ctctaacctt ctcagcaccg gaaccataac aattgctata aaaaacatac cacctagcca
     4201 tataatagtc gctagaacat gaattaggac aactaagtta taagaaaact gcacggcagt
     4261 gttttattaa ctcctctgct aatttctatt taagcagact ctctaataat tttttaagca
     4321 caacggcgtt attatgttct ttgtccttgg cactatagat taaagtaagt ttttcactag
     4381 tcgctatttt agtaagctca ataagagcta tctttttctc ctcatccttt cttagttccg
     4441 ttttatacct cttacaaaac tctttccatc gagaaggatc gtgagagaac catttcctaa
     4501 gtttagtgct tggagcaact tcctttagcc acaggtcaat cttaacttcc tcttttttaa
     4561 ttcccctcgg ccatagcctg tcaaccaaga cccgtattcc atcatctttc cccacttcat
     4621 cataaaccct ttcagtttta atcatgtgtt attcccttaa aaatcccttg ttagaaaagt
     4681 atagaatttt gtcttccccc taacccctca ttaataaatc aagtttgttt tcctacaggc
     4741 cgcactcgcg accaaacgtt ataaatgaaa agaagcccag ctaacacctg cgaagtgctg
     4801 cccaagacga ttaaccagtc aagtattctc gaatttatat aagaactagt aatttcacaa
     4861 atgaatcgaa ctacagttcc aagagttaaa aaccaataag ttatctccgc aagcccagga
     4921 ctatagtgca tttcatctcg tggacggggg aacatccaga tagccacacc catgataagg
     4981 gatattacaa aaccaaaaag cagggcatga gcatgtgctg taataagctg gggactcacc
     5041 cacttaccta gaatatattt gcttacaatc atatatagac caaggagtaa cccgactata
     5101 aagaaagcaa atgctgtttt tatgtaccgc ctcgatagtt tgaacataga ttcgccattt
     5161 agtttttagc aattcttatt tcttcatcca taataccatt tctaggtttg gtttgtcata
     5221 tcaaggtatc aatgaaag
//
LOCUS       DATDRL010000036         2666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2053188, whole genome shotgun sequence.
ACCESSION   DATDRL010000036 DATDRL010000000
VERSION     DATDRL010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2053188"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /gene="proA"
                     /locus_tag="VI935_01400"
     CDS             complement(<1..419)
                     /gene="proA"
                     /locus_tag="VI935_01400"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the phosphorylation of L-glutamate during
                     the proline biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HLE24288.1"
                     /translation="MLETKTAGISEGIQITKLIEDIASKAKWASRILAKASSNSKDQA
                     LLFMSDGLLSQSSFIIEENNKDKEDSKAKGFSTSFLDRLTLTPSRIKAIADGLKEIAS
                     LHDPVGEVVKMWRRPNGLLVGRMRIPLGVIGIIYEARP"
     gene            600..1403
                     /locus_tag="VI935_01405"
     CDS             600..1403
                     /locus_tag="VI935_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLE24289.1"
                     /translation="MPVIKIGSIRLNYELDGKGPAVVFINGLTMDVNGWYFQGPAFTQ
                     RYKILRYDCRGQGQSDKPEMDYSQEMHADDLKDLMDKLGIKKAHIVGISNGGMIAQHF
                     TLMYPEKVGGLVLVDTSSFIDTLLELIIKAWIKATEIGGSEFRYDMSLPALFAESYIK
                     KNPEAILVMRENNIRNNPQKPIVNLAKSCLKHNINDRTLEIKAPTLIIVGEEDILIPL
                     KYSKILHEKIKGSKLVIIKDCGHVPPIEKPEEFNKIVLDFLKDHDGLLK"
     gene            1480..2526
                     /locus_tag="VI935_01410"
     CDS             1480..2526
                     /locus_tag="VI935_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HLE24290.1"
                     /translation="MKAATYHGVRDIRVETVPDPKIQDSNDVIVRVTKSAICGSDLHL
                     YRGRVPIDEGFVVGHEFMGVIEDVGRSVKYFKKGDRVLVPFMIGCGVCVNCRNHVPAS
                     CTGGGGCFGFGKAWGDLPGGQAEHVRVPFADASLEKAPEGIPDEKILFLGDIFSTGYF
                     CAEWANIKPGYTVAVFGDGPIGLFAVASARLFGPALIITVGRHDFRLNIAKKMGSDIG
                     INSHKENAVEKILEITSGRGVDAVLECIGSDETLLDAFKIVRPAGTISFVGLFVQPVA
                     IPMLDFYLKDLTLRGGICSAKNYITKLMPLVEKGKIDTTAVITHDLPLSDTPRGYKLM
                     DSKEENAIKVVLTP"
BASE COUNT          841 a          523 c          563 g          739 t
ORIGIN      
        1 ggccttgcct cataaattat tccaatcaca ccaagaggta tcctcattct tcctactaga
       61 agaccgtttg gccttctcca catctttaca acctcaccaa cggggtcatg aagggacgct
      121 atctccttta acccatctgc tatagctttg atacgcgagg gagtgagggt aagacgatcg
      181 agaaaggacg ttgagaaccc ctttgcctta gaatcttctt tgtccttatt attctcctca
      241 attataaagc tcgactggga aagaagtcca tcggacataa ataggagagc ttgatccttt
      301 gagttggaag aggctttcgc caatattcta gaggcccatt tggctttact tgcaatatct
      361 tctattaact tagtaatctg aataccttca gagataccag cagtcttagt ttccaacata
      421 tatagtgaga ttaatccctt gccttataaa acataggcta ctattttcga ataatagtat
      481 atcacgtaat atattgtcaa gtacttttgt tttcattatt gttattaaat cttggatact
      541 taaatatgta agattaaccc attccttaaa atttagagag ctcaaccaag gggagtaaaa
      601 tgcctgtaat aaagattggc agcataagac ttaattatga actagatggt aagggccccg
      661 ctgttgtatt tataaatggc ctcactatgg acgtcaatgg gtggtatttt cagggtccag
      721 cctttactca aagatataag attctaagat acgactgccg tggtcaagga caatcagaca
      781 aacccgagat ggactatagt caggagatgc acgccgacga tctaaaagac ctcatggata
      841 agctaggcat taaaaaagcg catatagttg gcatatcaaa cggtggcatg atagcccaac
      901 atttcacact aatgtaccca gagaaggtcg gcggtttggt tcttgttgat acatccagtt
      961 tcattgacac ccttcttgag ctaatcatta aggcatggat aaaagcaacc gagatcggag
     1021 gaagcgaatt cagatatgat atgtcactac cagccctctt cgctgaaagt tacattaaga
     1081 aaaacccgga ggctattctt gtaatgaggg aaaacaacat aagaaacaat ccccaaaaac
     1141 ccatcgttaa ccttgcaaag agttgtctaa aacacaatat caatgaccgc accttagaga
     1201 ttaaagcccc aacccttata atcgtaggcg aggaagacat acttattcca ctaaaatatt
     1261 ccaaaatctt gcatgaaaaa atcaagggtt caaagctcgt cattatcaaa gattgcggcc
     1321 acgtaccgcc tatcgaaaaa cctgaagaat ttaataaaat tgttctagat ttcttgaagg
     1381 accacgatgg acttcttaaa taatgattat gccactacta gaaataacat tagataacta
     1441 gttataattc taatcaaaac aagagctagg aggtttcaca tgaaagctgc aacctatcac
     1501 ggtgttagag acataagggt tgaaacggtt cccgatccga agattcaaga ctcaaatgac
     1561 gtgatcgtca gagttacaaa aagcgcaatc tgcggctctg acttgcacct ctatcgaggt
     1621 cgcgtaccaa tagacgaggg atttgtagtt ggacatgagt ttatgggtgt catagaagac
     1681 gttggaagaa gcgttaagta ttttaagaag ggagacagag tgttagttcc ttttatgata
     1741 ggctgtggcg tctgtgtaaa ttgtagaaac catgtaccag cctcctgcac agggggcggg
     1801 ggatgctttg gttttggaaa agcttgggga gatttgcccg gtggacaggc tgagcatgta
     1861 agagttccct ttgcggatgc atcacttgaa aaagctccag aaggcattcc agatgaaaaa
     1921 atcctatttc ttggggacat cttttcgaca ggttatttct gtgctgaatg ggcgaatata
     1981 aagccgggat acacagttgc cgtatttggt gatggtccaa ttggactctt tgctgttgcg
     2041 tcagcaagac tttttggtcc ggcacttata ataacagtag gacggcatga ctttaggtta
     2101 aatatagcaa aaaagatggg gagcgatatc gggataaatt cacataaaga aaacgcagtt
     2161 gaaaaaattc tcgaaataac aagtggaagg ggcgtagatg cagttttaga gtgtattggt
     2221 tctgacgaaa cactgcttga cgcctttaaa atagtaagac ctgcaggaac tatttcattc
     2281 gttggactct ttgttcagcc ggtggcgatt ccaatgctgg atttttactt aaaagacctc
     2341 actttaagag gaggtatatg ttcggcgaag aattatataa caaagcttat gccccttgtt
     2401 gaaaaaggaa aaattgacac tactgctgta atcactcacg atcttcccct aagcgacacc
     2461 ccaaggggat ataaacttat ggacagcaaa gaggaaaatg cgataaaagt cgtgcttacg
     2521 ccttaaacga cttaatccca gagagacttt taggttctct gggatttcct tatatctact
     2581 tgcatataaa atcctaagtt ctgatctaca tattcaggtc tacaaaaacg ctttttacct
     2641 gagtataaag atccatagcc tgcatc
//
LOCUS       DATDRL010000037         2260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1791293, whole genome shotgun sequence.
ACCESSION   DATDRL010000037 DATDRL010000000
VERSION     DATDRL010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791293"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VI935_01415"
     CDS             <1..403
                     /locus_tag="VI935_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLE24291.1"
                     /translation="SIFGQAGPLIGSAPIWYGMQLLQRSVGDKKAREIVYLCNRYTAK
                     EAQEMGWVNKVVPKDKLDEEVDAWCKKILEMSPQSLRIAKFQINFASDTASPQITHGL
                     ELARFFLKSPEMVEGATAFIEKRKPDFYKVR"
     gene            467..1825
                     /locus_tag="VI935_01420"
     CDS             467..1825
                     /locus_tag="VI935_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24292.1"
                     /translation="MKDLESPCGCGSGKKFGACCNITKHVVDISQYKLDQAERDLRLK
                     LVDFTHRPEIQAQIGEAFYIWKNDPELLTENTGDEDDIDDLTFAKFFDWFVYDFKLIV
                     IGKRVIERFYDEEIHNLSDVEKTLIAEWFQNLYSFFEVEEVVPNEGCRIKDIFTGDVF
                     QIKDSSSSQQITPHDIVAGRPLKVGKNTYFSSVISVYPQAFKNLIIDFFNLQFKDYKK
                     NFGRKSTVKGYLKDLGFLIGQYIEDVVGHSQFLTPEGDELVFASAIYYVKNYEMAIKK
                     LKNIKSFVEIEGGTDDLRVFTWERKRGKNRTVVTIEVENDKLRIDCYSLNSLTDTKKI
                     VEKNLNEFIEHKRDSIKHLDLNRDKELNKTSILINLPSGVSTKRELDTILDEHYDGWI
                     DKPLSALRGKTPREALETKHGREILNSVLNELEGLYKHARERGEPYYDIKKLRKKLRL
                     ES"
     gene            complement(1861..>2260)
                     /locus_tag="VI935_01425"
     CDS             complement(1861..>2260)
                     /locus_tag="VI935_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24293.1"
                     /translation="LISYAKTGFSYNGLIEYYRGVESGYPKTYLELLETTHFHIFSMS
                     VVFLILAHVFLMSSWSYRLKIMVIIISFLAMLAEICSPWLITYVSPKFAVLMIISGTI
                     IALSMLLYIVVPIYEMWFIKLNNGSIQNSS"
BASE COUNT          776 a          399 c          462 g          623 t
ORIGIN      
        1 ttccattttt ggtcaagccg gtccattgat tggcagcgcc ccaatctggt acggcatgca
       61 actcctgcaa cgctcagtgg gtgataaaaa ggcgcgtgag atcgtgtatc tctgtaaccg
      121 ctatactgcc aaagaagcac aggaaatggg ctgggtaaac aaggtagtac cgaaggacaa
      181 actcgatgaa gaagttgacg cttggtgtaa aaagatactt gagatgagcc ctcagtccct
      241 tagaatcgct aagtttcaaa ttaactttgc gtccgacacg gcatcccctc agattactca
      301 tgggcttgag cttgcccgat tcttcctcaa atcccctgag atggtcgagg gcgctacagc
      361 atttatcgag aagcgtaagc ccgatttcta taaggttcga taaaaaaacc acaccgagaa
      421 tataatgatt gacactattt tcaatataat ttagctttta acatttatga aagacttaga
      481 atctccttgt gggtgtggga gtggaaaaaa gtttggcgcc tgctgcaata tcacaaaaca
      541 tgtagttgac atcagccaat ataaacttga tcaggctgag agagatttac gcttaaagct
      601 tgttgatttt acacatcgcc ctgagataca ggcacaaatc ggtgaggcat tttacatctg
      661 gaagaacgac ccagaacttt taactgaaaa cacgggggat gaggatgata ttgatgacct
      721 tacatttgca aaattctttg actggtttgt ctatgatttt aaattaattg ttattgggaa
      781 aagggtaata gagcgatttt atgatgaaga aatacacaat ctatcagatg ttgaaaagac
      841 attaatcgct gaatggtttc agaacctcta tagcttcttt gaggtagaag aagttgttcc
      901 aaatgaggga tgcagaataa aggacatatt cacaggtgat gtatttcaga taaaagactc
      961 ttcatcgtct cagcaaatca ccccccacga tatagttgcg ggaagacccc ttaaagtagg
     1021 aaagaatacg tacttttcaa gcgtgatttc agtttatccc caggcattta aaaatcttat
     1081 tatagatttt tttaatcttc agtttaagga ttacaaaaaa aactttggaa gaaaaagcac
     1141 ggtaaagggc tatttaaaag acttggggtt ccttataggc cagtatatcg aagatgtagt
     1201 tggacattct cagttcttaa cacctgaggg agatgagctc gtttttgcct ctgcaatcta
     1261 ctatgtaaaa aattatgaaa tggccataaa gaaactaaaa aatattaagt cttttgtaga
     1321 gatagagggg ggtacagatg atcttagggt ctttacttgg gagagaaaac gaggcaaaaa
     1381 tagaaccgta gttacaatag aggttgagaa tgataaacta agaattgatt gctattcact
     1441 taattcacta actgatacaa aaaagattgt tgaaaaaaac ctcaacgagt ttatcgaaca
     1501 taaaagagac tctataaaac atttagactt aaatcgtgat aaagaactta ataaaacctc
     1561 tatactcatc aatctaccct caggagtcag cactaaaaga gaacttgata ctattcttga
     1621 cgaacactac gatggatgga ttgataaacc tcttagtgca cttcgaggta aaacaccaag
     1681 agaggcttta gaaacaaagc atggtagaga aatactcaat tctgttttga atgaactcga
     1741 gggcctatac aaacatgcaa gggaacgtgg cgagccctac tacgacataa aaaagctcag
     1801 gaaaaaactt aggcttgaat cctgaataaa gggttttaaa gaagtttcaa gttcttattt
     1861 ctagctagaa ttttgaatac ttccattatt caactttata aaccacatct catatatagg
     1921 aaccactatg tatagcagca tgctaagagc aatgattgta cccgagataa tcattaagac
     1981 tgcaaactta ggagacacat aagttataag ccaaggactg catatctcag caagcattgc
     2041 taaaaatgag ataattatca ccattatttt caagcggtaa ctccaagagg acataaggaa
     2101 tacgtgggca aggattaaga agacaacaga cattgagaag atatgaaaat gggttgtctc
     2161 taatagttcg aggtaggtct ttgggtaacc gctttcgaca cctctgtagt attctatcaa
     2221 tccattatag ctaaagcctg tttttgcata agagattaat
//
LOCUS       DATDRL010000038         2120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1791071, whole genome shotgun sequence.
ACCESSION   DATDRL010000038 DATDRL010000000
VERSION     DATDRL010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791071"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /gene="purH"
                     /locus_tag="VI935_01430"
     CDS             complement(<1..1340)
                     /gene="purH"
                     /locus_tag="VI935_01430"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HLE24294.1"
                     /translation="MKKAERAIISVWKKEGIGKFAKELKELGIEILSTGGTSKIIRDA
                     GVEVTEISDYTESPELLGGRVKTLHSKIHAGILAARDEEAHLKDMVRQGIKPIDIVVV
                     NFYPFENVIKNEDVVLSEAIENIDIGGPTLLRAAAKNYKYVTVVTHPEDYKTVIDELK
                     RNKGSLSVEKNLRLAQKAFSYVARYDAAISNYLGALEDGKRTKFPESLTLHFEKQMKL
                     RYGENPHQEGSFYVQPGIEEPCVSNAIQLQGKELSLNNIYDADAALEAVKDFSETACV
                     IVKHNNPCGVATAETPYQAFLKARECDPESAFGGIVALNAEVDETTASELSSMFLEVV
                     IAPSYTEKALSSLSTKQNLRVMKTLILTRKPKSGLDIKKVVGGALVQDRDVTADEDFI
                     NFKVVTKREPTDEELLALKFAWKVCKHVKSNAIVFAREGQTIGIGAGQMSRIDSV"
     gene            1762..>2120
                     /locus_tag="VI935_01435"
     CDS             1762..>2120
                     /locus_tag="VI935_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HLE24295.1"
                     /translation="MRDQRILNLKVGIFVLLSLVIFLVFIFALGGESGLFKRTYKVQT
                     SFTNTAGLSQGAAVRLSGVLIGSVKEIQFPENPDDNFIKVLMEVNEEGIRRIGPDAIA
                     TIRTEGLLGDKYIEIIKG"
BASE COUNT          562 a          439 c          422 g          697 t
ORIGIN      
        1 acagagtcaa tgcggctcat ctgtcctgcg ccaattccaa tggtttggcc ctcgcgcgcg
       61 aatactatgg cgttagattt aacatgctta cataccttcc atgcaaattt aagtgctagg
      121 agctcttcat cagtaggttc acgttttgtt acaaccttaa agtttataaa gtcttcatca
      181 gccgtaacgt ctctatcctg aacgagtgcc ccacccacta ccttcttgat atcgagtcca
      241 ctcttaggct ttcttgttaa aatcaaagtc ttcatgaccc ttaaattctg cttggttgat
      301 agtgaactaa gagctttctc agtgtagctt ggtgcaatta cgacttctag aaacatagaa
      361 ctaagctccg atgcagtcgt ctcatctact tctgcattaa gagcaacgat tcctccaaat
      421 gcgctttctg gatcacattc tcttgcttta agaaacgcct gataaggagt ttccgctgtt
      481 gcaactccac atggattatt gtgctttact ataacacaag cggtttctga aaaatctttt
      541 acagcctcta atgcggcatc tgcgtcatat atattattca gtgaaagctc ttttccctga
      601 agttgaatgg catttgatac acagggctct tctatacccg gttgaacgta aaatgaacct
      661 tcttgatgag ggttctcacc atatctaagt ttcatctgct tttcgaaatg aagtgtcaag
      721 ctctctggga atttagttct tttcccgtct tctagcgcac ccaagtagtt agaaattgca
      781 gcatcatacc gtgctacata ggaaaaggcc ttttgtgcga ggcgcaaatt cttttcaacc
      841 gataaagaac ctttgtttct ttttaactcg tctattacag ttttataatc ttcaggatgg
      901 gtaactacag tgacgtattt gtagttctta gcggctgcac gaagaagtgt cggcccccca
      961 atatcaatgt tttcaattgc ctcgcttaaa actacatctt catttttaat tacattctca
     1021 aaaggataga agttcacaac gacaatatct atgggtttga ttccttgtcg taccatatct
     1081 ttaaggtggg cttcttcgtc acgagccgcg aggataccag catggatttt ggaatgcagt
     1141 gttttaactc tgcctcctag aagctcagga gactcggtat agtctgaaat ttctgttact
     1201 tcaactcccg cgtcgcgaat aatttttgat gtgcctccag tagaaagaat ttcaatacca
     1261 agttctttaa gttcctttgc gaatttacca attccttctt tcttccatac acttataata
     1321 gctctttctg cctttttcat ttttggtact ccttcgttcc tggaatggat tttggggttc
     1381 gtgactagtg gttcgtggtt cggtacgaga tccgcaagtc acgaaccacg attcactctt
     1441 taaacaaacc aaaaattaac atatcttttg agaagttgtc aatttcaaat tataatttct
     1501 aaattgagga ttccctataa cttgctacag ggctactact gttgtcatcg cgacgcccga
     1561 ttaagtcttc ggggggaagc gatctgtttg agattcctca cttcgttcgg aatgacactc
     1621 tgtgtcagat tgcttcgtca cggagtttat cctgagcgag gtcgaaggat tcctcgcaat
     1681 gacagtcgtg acggtgttat tataagacga taccctgcag cttgctgaag ggttcttcaa
     1741 ttcttaaata gggagtaatt catgagagac cagagaatct tgaatttaaa agtgggaata
     1801 tttgttttgc tctcgcttgt tattttttta gtttttattt ttgcactagg tggtgaaagc
     1861 ggtttattta aaagaaccta caaggttcaa acatccttta caaatactgc tgggctctct
     1921 caaggagctg ctgttagact ttctggggtg ttaattggta gtgttaagga aattcagttt
     1981 cctgaaaatc cagatgataa cttcataaaa gttttgatgg aggtaaacga agaaggcata
     2041 agaaggatag gtcctgatgc aatagcaaca attagaaccg agggtctttt aggagacaaa
     2101 tatatagaga ttattaaggg
//
LOCUS       DATDRL010000039        11269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1253729, whole genome shotgun sequence.
ACCESSION   DATDRL010000039 DATDRL010000000
VERSION     DATDRL010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253729"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VI935_01440"
     CDS             complement(<1..404)
                     /locus_tag="VI935_01440"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLE24296.1"
                     /translation="MNRYLGISGDYQYRAIHEGIFIQRVWHLNKIYLVDEKFNITSND
                     IVLEIGCGSGNLLSRMAKRALLVTGVDISEASIEFAEQQCAGLVNVDFQVNTINKMEL
                     PEGYYSKVVCQEVIEHLFEDEINQLMSKSYKT"
     gene            complement(415..1335)
                     /locus_tag="VI935_01445"
     CDS             complement(415..1335)
                     /locus_tag="VI935_01445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLE24297.1"
                     /translation="MREIKKITLVLPTYNEEKNIALMVEEVVPLCTKFSSDYQIIFVD
                     DGSEDDTTLEIKKATDKNDKIKFIFFTRNFGHDSAMRAGIDYADGDFLIVMDADLQHP
                     IETAEHMIGLWRKGFDVVHGVRSRSKKLTDIFAFYFYKLFNFVSNIRLYENATDFVLL
                     DKKVYEFIKEISETTFFFRGLVTWIGLRQTTVSFVPPPRIHGTTKYSFTKLINLSLDA
                     VTSFSIYPLRILSKLGFLIMCLSFCYALVAIGRRLFFETPFGFTAILVAILFLGGMNI
                     LAIGVIGEYIGKILLETKNRPKYLVKEKKV"
     gene            complement(1438..3408)
                     /locus_tag="VI935_01450"
     CDS             complement(1438..3408)
                     /locus_tag="VI935_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24298.1"
                     /translation="MEFVIAVVFLITSYLSGYLLTVRFFPNFKSLLCIASAYILGTLI
                     SVWLVLILSLILHPFTEEAITVGFILATAFLVAFTIHQRALFKAPLGLRPSHVIFFII
                     AFLYSWILFSSTFDYDEKLHEIKIAGLIWSDYGFHIPLIRSFSLGNNLSLEHPLYAHE
                     SIRYHFLFDFMVGVQEKMGIPLDYAINIPSALFWTCLLISIYYLSKKLFFGSRFVGFV
                     SVALFLLNSSLSFVEFINKYPPKSLESLINSWWHLPGYVAFGPWDGNIISAFWNWNIY
                     INQRHLAFGYTLVILVLIHYIDEHITKKGETTYRQKAFVGLTTGLLVLWHSQAFICLF
                     GLLGLFFLLFPGRKKSLVAVAVAFLIALPQILWLQKSSPNFESHFSLSPGYLVVSHLI
                     PIDFMPYQFLNKEISYILSFIRYWFFNLGLSFIIIPVSFFLVDTQRKKIFLMFLSLFI
                     IGNLFRFSPDMPGNHKFFNLWLILSNGFTAYLLYRLFHLGWLGRIATVVLLFFLTVSG
                     LVDAIPIKTDVTITFSDVQKQPLAKWVLKNTDTNEVFLTSFRIYNPVSFAGRRTMQGW
                     PYFAWGAGINTLKRESIGKKIYETNSKEGLCTLLRENRIDYIQTEKQLENNPVFKINH
                     EFFNTNFKPVFFDRRSHLQERVFATKDMCPQD"
     gene            complement(3396..4757)
                     /locus_tag="VI935_01455"
     CDS             complement(3396..4757)
                     /locus_tag="VI935_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015335932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLE24299.1"
                     /translation="MTTKRIIIIGAGPTGLGAAYRLRELGYTNWKIYEKNTYLGGHAS
                     SHQDENGFVWDEGGHVIFSHYKYFDDLIDKLLGKDYLEHMRESWIRMMGLWVPYPFQN
                     NIRYLPKEAQVKCITGLINTQNKAGKSSLNFKEWILETFGEGITEYFMHPYNLKVWAT
                     PLELMDKNWIAERVSVVNFKRVIENIIYERDDVNWGPNNKFKFPLYGGTGEIYRRLEP
                     YMEGNVAYGKKLVELDLKKKDVRFQDGSVDRYDFLINTSPLDRLVEIIRPVPHELRDE
                     VRRVIKHSSMFVVGIGIRKRLESNKCWMYFPEDTSPFYRVTYFSNYSPNNVPEGRVDT
                     YCSMMCEVSYSEFKRKNKDTIVEDTIQGLINTGMINDREKKLIVSKHLMDVDYAYPTP
                     TLERDIVLRAIQPFLMERGIYSRGRFGAWKYEIGNMDHSVMQGKEAVDCILEGTPEVT
                     WSL"
     gene            complement(4779..5759)
                     /locus_tag="VI935_01460"
     CDS             complement(4779..5759)
                     /locus_tag="VI935_01460"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HLE24300.1"
                     /translation="MSKIIGIAFSVIFLILALYRANFAQIGGALIGAKIGWLVLAAAV
                     YICSFIPRTLRWQRLLLPVRPLRFYQLLPVILVGYMANNLLPMRMGELFRAYFLKEKK
                     EVSGSSALATILVERVCDGLTLVLVLAVVLVFFPQKEWVNILGWTAGAVFFTGLIGTI
                     LLPRYGGLIQGAPLLSSLQDKPWGRWLITKFGAFLIGLRGLSSPADFAVIGTLSALIW
                     FVEGVVLYLVTLAFGLSLSPAGIALVLVIINFSTMIPSGPGFIGTFQYAFVLSFGLLG
                     IAKETAIAVSIATQLVFFSIVNPVGIGLLWKHNLSLRRAQEVMVKLKDLS"
     gene            complement(5962..6699)
                     /locus_tag="VI935_01465"
     CDS             complement(5962..6699)
                     /locus_tag="VI935_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLE24301.1"
                     /translation="METTDPSKLKSVSVVMPVFNEEGIIEKTVQDFYSEIVANIPGAE
                     MVIVDDCSTDKSPDILKKLVEELPSIRVLRPAKNGGHGKALRLAFENVRCDLIFHTDS
                     DYQNDPKDFWRLYKEIEGNDLVIGYRAVRHDPLPRLIITRLVRFVNFVLFGFHIRDAN
                     SPFKLIRRNCLEDCLKEISTEAFAPSISLAVTARWKGYRLKEVPVTHLPRKTGRVSIV
                     KWKLIKACFRTLREIYELRRGLSNSST"
     gene            6980..8770
                     /locus_tag="VI935_01470"
     CDS             6980..8770
                     /locus_tag="VI935_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HLE24302.1"
                     /translation="MFEERIFYGTLIGLVSFTWIGFIEALIFGLDLKTIVLTSSVLVL
                     ITLLIFYIKLYKRPIELSYTTGIFHSEVLFPIFTLCCIFFPVLHILFSRVILWKENGL
                     NTGLAYNLSDLPFHWSIINSFLYGDNFPPDNTIFSGYPLRYYFLSDFFTALLMKSGLS
                     LWGAFTLQGIMLSILLVGILYLLTYRLTQNKMAAAITPFLFFFNGGLGFIQFFKDLIS
                     DRGNLINFFLFVKDYTNIGDLGYRWINTTTSLLVPQRPFLFGFPTSIMVLTMLWVGIK
                     RQSRGLFTLAGVIAGTLPFFHVHSYISLGIISAVLFLLFPSLRWLWFFIPAGVLSLPQ
                     IFYLMPRGESAGHFFNIHLGWMANEDNVFLFYLKNSGLFIPVLVGTLIFSKNLSSSQR
                     KFAIPFILVFLITNVIQFTPWDWDNVKVLIYFYIGSIPFVAYGLASLWKNTYKIIPIV
                     LMLTLVFSGIISVARTTVSYYSENNNEEIELAETIKKITEPRSRFLTAPVYNHFILLT
                     GRSILHGFAGSLWGQGVDFSKRLEDIRKMYRGDRDAKELLDQYKVDYVVIGPPEIREK
                     LEPNLEFYKNNFPIIVQSENYYVFKSRSRN"
     gene            complement(8877..9572)
                     /locus_tag="VI935_01475"
     CDS             complement(8877..9572)
                     /locus_tag="VI935_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLE24303.1"
                     /translation="MISLSVVLPAYNEAKNIQKTINDAVFYLEKKVRDYEIIVVNDGS
                     VDGTREVVERLSLSNPKIALVNHQKNLGYGSALRTGFERASLDYIFFMDSDGQFDISD
                     IDRLIPFIKDYEVIVGYREKRADPFIRSLNTWLYHLFIRLLFGLKIRDMDCAFKLFPR
                     TAFQAIRPIKSGGALFSAELLIKLGMKGLEIKEVPVRHFPRRFGKQTGANLRVILRMF
                     KECRKLRDELRVL"
     gene            complement(9730..9963)
                     /locus_tag="VI935_01480"
     CDS             complement(9730..9963)
                     /locus_tag="VI935_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HLE24304.1"
                     /translation="MSRLPIVNFKIMEKVLLHLGFEAVRQKGSHVFYRHFDGRTTTVP
                     NHPGRDLARPLIREILREIELTPERFREGLKKI"
     gene            complement(9960..10178)
                     /locus_tag="VI935_01485"
     CDS             complement(9960..10178)
                     /locus_tag="VI935_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLE24305.1"
                     /translation="MRTFTAYVEFDPETKLYVGVVPGIPGAHTQGVTLDELQKNLKEV
                     LELCLEEYKGAKEDLPRFVGLQLIEIAV"
     gene            complement(10865..>11269)
                     /locus_tag="VI935_01490"
     CDS             complement(10865..>11269)
                     /locus_tag="VI935_01490"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HLE24306.1"
                     /translation="SDVFAATWGDYNNDGFMDVFLNGHSDKPLMENSGTQNNFLIIKL
                     VGNGEKTNISAIGTRVEVSSSKGIQVREVSGGRGCCEQDMLPLHFGVGNENVVEIQVQ
                     WTDGEICTFSNVNVEGGPLFQISESNCTISEF"
BASE COUNT         3329 a         2455 c         2198 g         3287 t
ORIGIN      
        1 aatgttttat aggacttaga cataagctgg ttgatttcgt cttcaaaaag atgctcgatt
       61 acttcttgac agaccacttt actgtaatag ccctccggta gctccatctt gttgatagtg
      121 ttcacttgaa agtcaacatt aactaagccg gcacattgtt gctcggcaaa ctcgatggac
      181 gcctcggaaa tgtcgacacc cgtcactagc aatgctcttt tagccattct tgataaaaga
      241 ttaccagagc cacatccaat ctctaacaca atatcattgg atgtgatgtt aaacttctcg
      301 tcgaccaaat aaattttgtt aagatgccac actctctgga tgaaaattcc ttcgtgaata
      361 gctcgatatt gataatcacc agaaattcct aaatacctat tcatatccga ttgcctacac
      421 ttttttttct ttgacgagat atttaggacg atttttagtt tctaaaagaa ttttaccgat
      481 gtattctccg ataacaccta tggctaatat gttcatcccg cctaaaaaga gtatagcaac
      541 taaaatagca gtgaaaccga aaggggtttc aaaaaacaat cttcgaccga tagctacgag
      601 ggcataacaa aaactcaggc acataatcaa aaaaccgagc tttgataaaa tgcgaagagg
      661 atagattgag aaggaggtga ccgcatcgag cgataagttg attaattttg taaaagagta
      721 tttggtggtg ccatgaatgc gaggcggtgg tacgaagctc accgttgttt gtctcaatcc
      781 tatccaggtg accaaacccc taaaaaagaa ggtcgtttct gaaatctcct tgatgaattc
      841 atacactttt ttatcaagaa gcacaaagtc tgttgcattt tcataaagcc gtatattaga
      901 gacgaaatta aaaagtttat agaagtaaaa ggcgaatatg tcagttaatt tttttgatcg
      961 tgacctaacg ccatgaacca catcaaatcc ttttctccag agtccgatca tatgctcggc
     1021 ggtttctatc ggatgttgta ggtccgcatc cataacaatt aaaaaatccc catcggcata
     1081 atcgattcct gcacgcattg ctgaatcgtg cccaaagttc ctggtaaaaa agataaattt
     1141 tattttatca tttttatccg tggctttttt tatctcaaga gtcgtatcat cctctgaacc
     1201 atcatcaaca aaaattatct gataatcaga ggaaaacttg gtacacaagg gcaccacctc
     1261 ttcaaccatt aaagctatat ttttttcttc gttatacgtg ggtaaaacaa gggttatttt
     1321 tttaatttct ctcatcttta ttgattttca acggatcgtt gtagtggtta caacagatga
     1381 taacatagta agtggctgat taacaaacga aagtagtgaa cataaaacta ctaatctcta
     1441 atcttgggga cacatatctt tagtcgcaaa aacccgctct tgaagatgag atcttcggtc
     1501 gaaaaatacg ggcttaaagt tggtgttaaa aaattcatga ttaattttaa atacgggatt
     1561 attctccaat tgtttctccg tctggatata gtcaatccta ttttctctca gcagggtaca
     1621 gagtccctct ttggagttag tttcatagat tttttttcct atactctctc ttttcaaagt
     1681 attaataccc gccccccaag caaaatatgg ccagccctgc atagttcttc tgcccgcgaa
     1741 ggatacaggg ttgtaaatcc tgaaggatgt gagaaaaact tcgttcgtgt ctgtattctt
     1801 cagaacccat tttgctaagg gctgtttctg tacatcagag aaggtaatag taacatcggt
     1861 ttttataggt attgcatcaa ctaacccaga gactgtcaga aagaaaagta gaacgactgt
     1921 ggctatgcga cccaaccaac cgaggtgaaa aagccggtag agtagatacg ctgtaaaccc
     1981 attactaagg atgagccaaa ggttgaaaaa cttgtggttg ccaggcatgt cgggactaaa
     2041 ccgaaagagg tttcctataa tgaacaggct taaaaacatg agaaaaatct tctttctttg
     2101 agtatctacc aagaagaagg agacaggaat gatgataaaa cttaagccca gattaaagaa
     2161 ccagtagcgt ataaaggaga gtatgtaact tatctccttg ttaagaaact gatagggcat
     2221 aaaatctatg ggtattagat ggcttacgac taaatagccg gggctgagtg aaaaatggct
     2281 ctcgaagttt ggggagcttt tttgaagcca gagtatctgt ggaagcgcaa taaggaaggc
     2341 aaccgcgaca gctaccaggc ttttcttcct ccctgggaaa agtaaaaaga aaagacccaa
     2401 gagtccaaaa aggcatataa aggcttgact gtgccagagg actaacaagc cagttgtcaa
     2461 cccgacaaaa gccttttgcc tgtaggttgt ctctcccttt ttcgtaatgt gctcatcaat
     2521 ataatgaata agaacgagaa taacgagtgt gtaaccaaag gcgagatgcc tctggttgat
     2581 gtatatattc cagttccaaa aagccgatat gatattgccg tcccagggac cgaatgcaac
     2641 gtacccggga agatgccacc atgagttgat aagtgactca agagacttag gaggatattt
     2701 attgataaac tcgacaaaag aaagcgagct attcagtaaa aaaagagcta cggagacgaa
     2761 gccaacaaat cggctcccaa agaaaagctt ttttgatagg tagtatatag atattaggag
     2821 gcaggtccaa aataaggcac tcggaatatt aatcgcataa tcaagaggta tccccatctt
     2881 ctcctgcact cccaccatga aatcgaataa aaagtgataa cgaatgcttt catgcgcgta
     2941 gagtggatgc tcaagggaga gattgttgcc gagactgaaa gagcgtatta aaggaatatg
     3001 gaagccgtag tcgctccata taagccctgc gatctttatc tcatgtagct tttcgtcgta
     3061 atcaaatgtg cttgagaaga gtatccagga gtaaaggaaa gcaattatga agaagataac
     3121 atgggacggt cttagaccaa gcggtgcttt gaagagagca cgctggtgta tggtgaaggc
     3181 aacaagaaaa gcagttgcaa ggatgaaacc cacagttata gcttcttcag tgaacggatg
     3241 gagaattaaa gacaatatga gaacaagcca aacagatata agggttccta atatataggc
     3301 agaggctata cacagtaagc ttttgaaatt tgggaaaaag cgaaccgtaa ggagataacc
     3361 agatagatag gatgttatga ggaagactac ggcgattaca aactccatgt tacttccggt
     3421 gtgccctcca gaatacagtc aaccgcctct ttgccctgca taaccgagtg gtccatattt
     3481 ccaatttcgt atttccacgc gccaaatcta ccccttgaat aaattcccct ctccatcaaa
     3541 aagggctgaa ttgccctcag gactatatcc ctttcaaggg tcggggttgg gtaggcataa
     3601 tctacatcca ttaagtgctt tgatacgatg agcttctttt ctctatcatt aatcatgccg
     3661 gtatttatta gtccttggat tgtatcctca actatcgtat ctttattttt acgtttaaac
     3721 tcggagtagg aaacctcgca catcatggag cagtatgtat ctaccctccc ctccgggacg
     3781 ttattcggtg aatagttgga aaagtaggtc accctataaa atggggacgt atcctcagga
     3841 aagtacatcc agcatttgtt actctcaagt cttttcctga ttccgatacc tacaacaaac
     3901 atactgctgt gtttgatcac tcttcttact tcatccctta gctcatgagg gaccggtctt
     3961 attatttcta ccaacctatc aagaggagag gtgtttatta aaaaatcgta tctatccact
     4021 gagccgtctt ggaacctgac atccttcttc tttagatcaa gctcaacaag cttcttacca
     4081 taagcaacgt tcccttccat atagggctct aacctgcgat atatctcacc ggttccccca
     4141 taaagaggga acttaaactt gttattaggc ccccagttaa cgtcgtccct ctcgtagatt
     4201 atattctcta taaccctctt aaaattaacc acgcttaccc gctcggctat ccagttcttg
     4261 tccatgagtt ctaggggagt tgcccagacc ttcaaattat agggatgcat aaagtactca
     4321 gttattcctt ctccgaaggt ctcgaggatc cactctttaa aattaagaga gcttttgccc
     4381 gctttattct gtgtgttaat tagtcctgta atgcatttta cctgcgcctc tttaggtaga
     4441 taccttatat tgttttgaaa aggatatggt acccatagcc ccatcatgcg aatccaactc
     4501 tcccgcatgt gttctaaata atccttcccc aatagtttat caattaagtc gtcaaagtat
     4561 ttataatgtg agaatatgac atgcccgcct tcatcccaga caaacccgtt ttcatcctga
     4621 tgagaagagg catgaccccc aaggtaggtg tttttttcat atatcttcca gtttgtgtaa
     4681 ccaagttcac gaagtctata agccgctcca agccctgtag gacctgcacc aattattata
     4741 attcgcttag ttgtcataag actccctttc ctcgaggttc aagataaatc ttttaatttt
     4801 accataacct cctgcgctct tctgagcgag aggttatgtt tccagagcaa acctataccc
     4861 acagggttca ctatgctgaa aaaaaccagt tgtgttgcaa tggagacagc gatggctgtc
     4921 tcttttgcga tcccaagcag cccaaaagaa agaacaaagg catattgaaa ggttcctata
     4981 aaccctggcc ccgatggaat catcgtgctg aaatttataa tcacaagcac taaagcaatg
     5041 cctgcagggg aaagagaaag accaaaagcc aaggtgacta ggtaaagaac cactccctcc
     5101 acgaaccaga ttaaggccga gagggtgcct ataactgcaa agtcggctgg agaagagagt
     5161 ccacgcagac ctataagaaa agccccgaac ttagtaatta gccatctgcc ccagggctta
     5221 tcttggaggc tggataaaag tggtgcccct tgtattagac ccccatatct tggcagaaga
     5281 atagttccaa taagccctgt gaaaaacacc gctcccgctg tccagccgag tatgttcacc
     5341 cactcctttt gcggaaaaaa aaccaggacg actgccaaga ccagcacgag cgtaagcccg
     5401 tcacagacac gttcaactaa gatagtggct aaggctgaag agccacttac ctctttcttc
     5461 tcctttagaa agtatgccct aaaaagctct cccatccgca ttggcaagag gttattagcc
     5521 atgtatccaa ccaaaattac aggtagtagt tggtaaaacc gtaggggtct tacaggcaag
     5581 agcaatctct gccaacgcag tgtacgagga ataaaactgc aaatatagac agcggcagcc
     5641 aaaaccagcc agcctatttt tgctccaatt aacgcccctc cgatttgggc aaaattggct
     5701 ctatagaggg ctaaaatcaa aaagattaca ctgaacgcta ttcctataat cttgctcatc
     5761 gtacgaaaca ctgactgaac actgagtgtg ccatactgga gtccttaggc tactctcagg
     5821 acttcgtcgt gatcagttcg aagagctcat acaagctcag tcaaacgata aacaccacga
     5881 agcatctttc aacgataaga ttattcacct ccgccgacga cttcgtcagt ctcagaacga
     5941 caatagccac aatcaagata gctatgtgct agaattactc aaccccctcc ttaattcata
     6001 aatctctcgt agagttctga aacaggcttt tattagcttc cactttacta ttgagacccg
     6061 tcctgtcttc ctggggaggt gagttacagg aacctccttc aggcgatacc ccttccacct
     6121 agccgtgacg gccagtgaga ttgagggagc aaaagcctcg gtactaatct ctttaaggca
     6181 atcctccaag cagtttcttc gaatgagttt aaaaggggag ttagcatctc gaatgtgaaa
     6241 gccaaataag acaaagttaa caaatcttac aagacgagtt ataatgagac gcggaagcgg
     6301 atcgtgacgg acggctctat atccaattac aagatcgttt ccttcaatct ctttatagag
     6361 cctccagaag tccttggggt cgttttgata gtcgctgtct gtgtggaata tgaggtcgca
     6421 cctaacgttt tcaaatgcaa gccgtagcgc cttcccgtgc cctccgttct ttgcaggtcg
     6481 caaaactcgg atggaaggaa gttcctctac tagtttctta agaatatcag gactcttatc
     6541 cgtagaacag tcgtcaacaa taaccatttc tgcgccaggg atattggcaa ctatctcaga
     6601 ataaaaatcc tgaaccgtct tctctattat cccctcctca ttaaagacgg gcatcactac
     6661 agaaacagat tttaatttgc tggggtcagt agtctccaat gtctaaacgg ttctctttat
     6721 gagtgtcatt caagccttta taatatatat ctccccgagt gcttactcaa gcatgcgtct
     6781 aaaagtttcc cttgctttct aattcctaat gtaatttgtc ttgcctattt gtctttggta
     6841 tatataataa tttttagtta tcaagccaga ctatggataa atagctttaa cccactctca
     6901 aatgctctta gcatttctct atatactaat ctccgtcatc tttggcttcg ccgtcaccct
     6961 tctcctcaag gaagaacttt tgttcgaaga gaggattttt tatggaacct tgatcggttt
     7021 agtttccttc acttggattg gctttattga ggcactgatt tttggtctgg atttaaagac
     7081 aattgtgctt acctcttctg ttttagtttt aataacccta ttaattttct atattaagct
     7141 ttataaaagg ccgattgaac tctcctacac tacaggcatt tttcattctg aggtgctttt
     7201 cccaattttc actttatgct gtattttttt ccctgttctt catatcctat ttagtagagt
     7261 aatactctgg aaggaaaacg gtcttaatac agggcttgcc tacaacctga gtgatctacc
     7321 tttccactgg agtataatta attctttcct ttatggggat aatttccctc cagataatac
     7381 aatttttagc ggctacccgc ttcgttatta ttttctctcg gatttcttta ccgctcttct
     7441 tatgaaatcg ggattaagcc tatggggtgc cttcaccctt cagggtatta tgctgagtat
     7501 actcctcgta gggatattat acttacttac ttatcgcctt acccagaata aaatggccgc
     7561 tgctataacc ccttttcttt ttttctttaa cgggggctta ggattcatac agttctttaa
     7621 ggatttgatt tcggatagag gaaacctcat aaattttttc ttattcgtaa aggattacac
     7681 taatattggg gacctgggat accgatggat aaacactaca acctctcttc ttgttcctca
     7741 aagacccttc ttattcggtt ttccaacgtc aataatggtc cttacaatgc tctgggtagg
     7801 tataaaaagg caatcaagag ggctttttac tctagcaggt gttatagcag gtacccttcc
     7861 tttttttcat gtacatagct acatttcttt agggataata agcgcagttc tattccttct
     7921 ttttccctcc ctcaggtggc tttggttctt tattccagcg ggggttctat ctctacccca
     7981 gattttttac cttatgcccc gtggagaatc tgctgggcat ttcttcaata ttcatctggg
     8041 atggatggct aatgaagata acgtcttcct gttttatcta aaaaattccg ggctctttat
     8101 ccccgttctc gtgggtacgc ttatatttag taaaaacctc tcctcctctc agaggaaatt
     8161 tgctataccc ttcatacttg tctttttgat cacaaatgtc atacaattta caccttggga
     8221 ttgggataac gtaaaggtat taatatattt ttacattggc tcgattcctt ttgtggctta
     8281 tggtttagcc agtctatgga agaacacata taagattatt ccgatagtgc tcatgcttac
     8341 gctagttttc tccggcatta taagtgtagc taggactact gtgtcgtatt actcagagaa
     8401 taataacgaa gaaatagaac tggctgagac cataaaaaag ataacagagc cgaggtccag
     8461 atttcttacg gcccctgtct ataaccactt tattcttctt acaggaagga gcatacttca
     8521 tggttttgca ggaagtcttt ggggacaagg ggtggatttt agtaaaagat tagaggacat
     8581 aagaaaaatg tacaggggag atagagatgc taaggagctt cttgatcagt ataaagtaga
     8641 ttatgtagta ataggacctc ctgagataag agagaagttg gaaccgaatt tagaatttta
     8701 taagaataat ttcccaatca ttgttcagtc tgagaattat tacgtgttca agtcgagatc
     8761 acgaaactga tttcatgact aaatgcttag aaatactctt taatgtgcca agtggttata
     8821 ccgcgatgca aaggcaagaa cgtaaaaagg gttcatgcct atttgctttt tcccctttat
     8881 aaaaccctaa gttcatctct cagtttccgg cactccttaa acattctcaa tataaccctt
     8941 agatttgcgc ctgtttgctt tccaaaccga cgaggaaagt gcctaaccgg aacctcttta
     9001 atttctaacc ccttcattcc taatttaata agcaactctg cactaaagag ggcgccaccc
     9061 gattttattg gtctaatggc ttgaaatgca gttctaggaa atagtttaaa cgcacagtcc
     9121 atatctctta ttttcaaacc aaaaagtaac cttatgaata aatggtaaag ccatgtgttt
     9181 agtgagcgaa tgaatgggtc agcccttttt tctctgtagc caactattac ctcataatct
     9241 tttataaaag gaataagacg gtcaatgtca cttatgtcaa actgaccatc gctatccata
     9301 aaaaatatgt aatcaagtga tgccctttca aacccggttc taagagcaga accataacct
     9361 aaattttttt ggtgatttac taaagctatc tttgggttag ataaagatag tctttctaca
     9421 acctctcgag ttccatcaac cgagccatca tttacaacaa tgatttcata atccctaacc
     9481 tttttctcga gataaaagac agcatcgtta atagtttttt gtatattttt tgcttcatta
     9541 taagctggga gcaccacaga gagagaaatc atgatgttgg attattgtaa catacatggc
     9601 gaatggattt gagcaagaga gcaggacaga aatgtcctgc ctatcgtgtg tgtggcaaga
     9661 atgttgtcta tcctatataa atcaacaata taccgatagg cggggttttc taaccccgtt
     9721 gacataagct tatatcttct tcaacccttc cctaaatcgc tctggcgtta gttcaatctc
     9781 acgaaggatt tcacggatca atgggcgggc aaggtctctt ccaggatgat tcggtacggt
     9841 ggttgtccta ccgtcaaaat gacggtaaaa gacatgactt cctttctgtc tcacagcttc
     9901 aaaaccaagg tgaaggagta ctttctccat tatcttgaag ttaacgattg gaagacggct
     9961 catacggcta tctcaatcag ctgaagtcct acaaaccgag gtaggtcttc cttcgccccc
    10021 ttatattcct ccaaacataa ttctagcacc tctttgaggt ttttctggag ctcatctaag
    10081 gttaccccct gcgtatgggc gcccggaatg cccgggacca ctcctacata gagtttggtc
    10141 tcagggtcaa attcaacata ggcagtaaag gttctcaatt tacacctcct tctagaattc
    10201 tcttaataga atacatagca gaagagaacg gaacaagcct gccatgagcc tttagaaggg
    10261 aatgttccac ctatctttaa aatacatcga taggtgatgt tttcaaaata catcaataca
    10321 acgataggcg gggttttcaa accccgcaga aaaagcggga atggaaagtc aagccttcta
    10381 ttgtgtttcg gcgtgcaata acaaggtctt gctgtgattt cgaggagcgt tatttcgagc
    10441 gccgctttct ttgaaccgca gtgtattatt tgatgcgcag tgtgcatcaa atggaaatag
    10501 gtggttcaaa gggaaataaa caaacgggaa attgaaataa aaggcgacga ggaggcaatc
    10561 caaaggaggg attgcttcgc taccgctcgc aatgacatca cggaattgtc attttattag
    10621 caatgacaaa tagtgctgcg agtagtgaaa cgacgaaaca atctaacacg aagtgtcatt
    10681 ccgaatgctt cgcgttggct cagtaaaggc ttcgtgaggg atctcaatcg gattgcctcg
    10741 ccattggctc gcaatgacag cgccctttct tgtcatttcc acgagaagcc gttcgaagga
    10801 gtttacaatg agcgaattcg aagtgctaag ggtaaactcc gcgacgaagc gattcaaaaa
    10861 atggtcaaaa ttcgcttata gtacaattgc tttccgaaat ctgaaacagg ggtccacctt
    10921 cgacattaac attactaaag gtgcatatct ctccatctgt ccactgaacc tgaatttcta
    10981 ccacattttc atttccaacc ccaaaatgaa gaggaagcat atcctgttca caacacccgc
    11041 gccctccaga gacctccctt acctgaatac ccttggacga actcacctca acccttgttc
    11101 ctattgcaga tatattagtc ttttccccat tgccaaccag cttaataata agaaagttgt
    11161 tttgtgtacc gctattctcc ataagaggct tatcactgtg cccgtttaaa aagacatcca
    11221 taaaaccgtc attattataa tccccccagg ttgctgcaaa aacatcact
//
LOCUS       DATDRL010000040         3879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_195425, whole genome shotgun sequence.
ACCESSION   DATDRL010000040 DATDRL010000000
VERSION     DATDRL010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195425"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VI935_01495"
     CDS             <1..543
                     /locus_tag="VI935_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLE24307.1"
                     /translation="RLNSYPHELSGGMCQRVMIAMALSCNPELLIADEPTTALDVTIQ
                     ASILELISELKEKIGMSILLITHDLGIISEVADKVYVMYAGKIVEEAPVINLFKNTRH
                     PYTVGLMNSIPSLHSRKERLRAIPGIVPGYKDLPIGCRFQDRCPMVIDKCRISEPPLR
                     RIENGHASACFRAEEVNAGV"
     gene            723..1691
                     /locus_tag="VI935_01500"
     CDS             723..1691
                     /locus_tag="VI935_01500"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HLE24308.1"
                     /translation="MERKELIKLLKLKKYFPIKSGIFSQVSNYVRALDGVDLSVFEGE
                     TLGLVGESGCGKTTLGKTAIKLLEPTEGVIFFNGRDITNLNSGEMRRLRREIQIIFQD
                     PYGSLNPRMKVESIVGEGLTIHKIITRKERRAEVKRLLETVGLREDVLSRYPHEFSGG
                     QRQRIAIARALAVNPKFIVCDEPVSALDVSVQAQIINLLLTLQEEFNLTYLFISHDLR
                     VVRHISDRVSVMYLGKIVELAESEEIYNNPLHPYTQVLLSSVPILDPETKRKIIILPG
                     DVPSPIDPPSGCRFHPRCPIAVERCKSEEPVLRDVGRGHMVACHLV"
     gene            1772..2287
                     /locus_tag="VI935_01505"
     CDS             1772..2287
                     /locus_tag="VI935_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HLE24309.1"
                     /translation="MKIRVSDIGETGVHIKTFRKPEWLTNIPELYSDEGDTHLSSNIN
                     VDLLLTKVLKEISVRGNIWFSIETPCARCLDTIDLILEPEVNLILLPEYLPREEDEDI
                     DFETYKGDEIDLGGYLRELIAISLPIKVLCGEGCKGLCPNCGVNLNLEGCFCKDNWVD
                     PRFAVLRNLKV"
     gene            2414..2596
                     /gene="rpmF"
                     /locus_tag="VI935_01510"
     CDS             2414..2596
                     /gene="rpmF"
                     /locus_tag="VI935_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HLE24310.1"
                     /translation="MALPKRKPSKSKSRKRRTHYKISHPTLSICPNCNELKPPHRVCP
                     HCGFYKGRTFKKETEE"
     gene            2604..3626
                     /gene="plsX"
                     /locus_tag="VI935_01515"
     CDS             2604..3626
                     /gene="plsX"
                     /locus_tag="VI935_01515"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HLE24311.1"
                     /translation="MKIALDAMGGDLAPRAVVEGALLALREIEGSVTLVGQKDEITRE
                     LSQFDEMGKEKIDIVHASEIVEMKEPITKAIRKSNSSIKVSAELVRRGEADAFVSMGH
                     SGAVMALGMITLGKISGIARPALCAVIPTLKGSSVLIDVGANVDSKPINLVQFAIMGE
                     AFSRIILERENPKVGLLSNGEEDIKGNETTKRTHSLLKECSINYIGYVEGKDLLLGDI
                     DIIVCDGFTGNVILKMGEGFVELLPKFIMQRALSNSEVVRYLPSIEEFEHLIREKFDY
                     NEYGGAPLLGVKGICIVGHGRSKAKAVKNAIRMAEELVRRDLVNKIEKEFTVNIKLSR
                     SLTNLG"
     gene            3633..>3879
                     /locus_tag="VI935_01520"
     CDS             3633..>3879
                     /locus_tag="VI935_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HLE24312.1"
                     /translation="MKAFIFPGQGSQYVGMGQDLYKEFITAKRTFEESSEVLRLDMAK
                     LCFEGDSEDLRRTANTQPAILTVSVAAWRVLDEEMGIK"
BASE COUNT         1240 a          650 c          922 g         1067 t
ORIGIN      
        1 agactaaaca gttatccaca tgagttgagc ggtggaatgt gtcagagggt gatgattgca
       61 atggctctat cgtgtaatcc agagcttctt atcgcagatg agcctacaac agcactcgac
      121 gttacaattc aggctagtat cttagaatta attagcgaac ttaaagaaaa aatagggatg
      181 tccattcttc ttattacaca cgatcttgga ataatctccg aggttgcaga taaggtttat
      241 gttatgtacg caggaaagat tgtagaagaa gcaccagtga taaatctatt taagaatact
      301 cgtcatccat atactgtagg gcttatgaac tcaattccta gcttgcatag caggaaggaa
      361 aggttgaggg cgattcccgg aatcgttccg ggttataaag accttccgat agggtgcagg
      421 tttcaggata gatgtccaat ggtaatagac aaatgtagaa tttctgagcc gcctcttaga
      481 cggattgaga atggacatgc ctctgcatgc tttagagctg aggaggtaaa tgcgggagtt
      541 taatgaaacg acagttaaac atgaagagtg aataaatggt gctgttgata gtgcacacgg
      601 aagcccgtct ttgcgaggag cgaagcgacg aagcaatctc aaacagtcaa ggaattgctt
      661 ccctcctctc ggtcgctcgc aatgacaact cctcgttcat tgggataaag tttagaatct
      721 aaatggaacg aaaagaacta attaaattac taaaactaaa aaaatacttc ccaatcaagt
      781 cagggatttt ctctcaagta agtaattacg taagggccct agacggagtg gatttgagtg
      841 tttttgaagg tgagacactt gggctagtgg gagaaagcgg atgtggaaag acaacacttg
      901 gaaagacagc tataaaacta cttgagccta ccgaaggagt tattttcttt aatggacgag
      961 atataactaa tttgaattct ggtgaaatgc gtcgcttaag acgcgagata caaataatct
     1021 ttcaagaccc ttatggatca cttaatccga gaatgaaggt tgaaagtatc gtaggcgaag
     1081 ggcttacaat tcacaaaatt attacgagaa aagaaagaag agctgaggtt aagaggctcc
     1141 ttgaaactgt aggtcttagg gaagatgttc tttctcgtta tccacatgag ttcagcggtg
     1201 gacagcgtca gaggatcgca atagctcgtg cacttgcggt aaaccctaag tttatagttt
     1261 gcgacgagcc agtttcagcc ctcgacgttt cagttcaagc acagataata aacctccttc
     1321 ttacgcttca agaagaattc aaccttacat atctttttat atctcatgac cttcgtgtcg
     1381 tgagacatat tagtgacagg gtatctgtga tgtatctagg aaaaatagta gaactggctg
     1441 aaagtgagga aatatacaat aatccgcttc atccctacac tcaggttctg ctttcttctg
     1501 ttcctattct agatccagag acgaagagaa agattataat tctccctggt gatgttccaa
     1561 gcccgattga tccaccttct ggatgtaggt ttcatccccg atgtccgatt gcggtcgaaa
     1621 gatgtaaaag cgaagaacca gttttgcgag atgtcggtag agggcacatg gttgcttgtc
     1681 atctagttta gattggagaa taggaatcaa cttgcaaata ccctttcttg tattatattt
     1741 tagaaaacaa attgtagttt agaaatataa aatgaaaatt agggtatccg atataggtga
     1801 gactggagta catataaaaa cgtttagaaa gcctgaatgg cttaccaaca ttcctgagct
     1861 ttattcagat gaaggggata cacatctttc ttctaacatc aatgttgatc ttcttctaac
     1921 gaaggtatta aaagaaatct cagttcgcgg aaatatttgg ttctcgattg aaacaccatg
     1981 tgcaaggtgt ctcgatacaa tagatttaat tctcgaacca gaggtgaatt tgattcttct
     2041 acccgaatat cttccgcgcg aagaggatga agatattgat tttgagactt acaaaggaga
     2101 tgagatagat ctaggtggtt atcttcgtga gctgattgct atttctttac ctataaaggt
     2161 actctgcggt gagggatgta aggggctctg ccctaactgc ggtgtcaact taaacctaga
     2221 ggggtgtttt tgcaaagata attgggttga ccctcggttc gcagtcctta gaaatctgaa
     2281 ggtttaggag taaatattca aacaagaatt ggcataaatg atcacgaatg aaaataggcg
     2341 ggatttatta gtgtaaattc gtgttcgttt gtgcataaat agtaacgttt agaacctaaa
     2401 agaggagatt ataatggcgc ttccaaagag aaaaccttct aagtcgaagt ctagaaagcg
     2461 aaggacgcat tacaaaattt cgcatccaac tctttctata tgtcctaatt gcaatgagct
     2521 taagcctccc cacagggttt gtccacactg tgggttttat aaaggaagaa cattcaaaaa
     2581 agaaacggag gagtaagagc ataatgaaga tcgctcttga tgcaatgggc ggggatttgg
     2641 ctccgagagc agtggttgag ggtgctcttc tagctttaag ggaaattgag ggctcagtaa
     2701 ctcttgtagg acaaaaagat gagataacaa gagaattatc tcaatttgat gagatgggaa
     2761 aagaaaagat agatattgtt catgcatcag aaatcgttga gatgaaagaa ccaatcacaa
     2821 aggcaataag aaaatccaat tcttccatta aggtttcggc agaacttgta cgaagagggg
     2881 aagcagatgc atttgtgagc atggggcatt caggagcggt aatggcactt ggaatgatta
     2941 ccttagggaa aatcagcggt attgccagac ccgccctatg tgcagtaatc cctacattga
     3001 aaggctcttc tgttttaatt gatgtaggag ccaatgtaga ctcaaaacct attaacttgg
     3061 ttcagtttgc aattatgggc gaggcgtttt caagaatcat ccttgagcgt gagaatccga
     3121 aagtaggcct acttagcaac ggtgaagagg atattaaggg aaatgagacc acaaagcgta
     3181 ctcactccct actcaaggag tgctcgatca actatatagg gtatgtagag ggaaaggacc
     3241 tacttttagg tgatatagat ataatagttt gtgacggatt tactggaaat gttattctca
     3301 aaatgggtga gggtttcgtt gagcttttgc caaaatttat aatgcaaagg gcgttaagca
     3361 attcagaagt agtaagatat cttccttcta ttgaggaatt tgaacacttg atcagagaaa
     3421 aatttgacta taatgaatat gggggtgcac ctcttttggg agtaaaaggt atatgtattg
     3481 tgggtcacgg aagatcaaaa gctaaggccg taaaaaatgc gattcggatg gctgaagaac
     3541 tcgtaaggcg tgacttggta aataagattg agaaagagtt cacggttaat attaaactaa
     3601 gtagaagttt aacaaactta ggctaaaatt ccatgaaggc atttatattt ccagggcagg
     3661 gatcacaata tgtaggaatg gggcaggatc tttataaaga atttatcact gcaaaaagaa
     3721 cgttcgagga atcgagtgag gtactcaggt tagatatggc aaaactctgt tttgagggag
     3781 actcagagga tttaagacgc acagcaaaca cgcagcctgc tatccttacg gtaagtgttg
     3841 ctgcatggcg agtactagat gaggaaatgg gtattaaag
//
LOCUS       DATDRL010000041         7291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3362166, whole genome shotgun sequence.
ACCESSION   DATDRL010000041 DATDRL010000000
VERSION     DATDRL010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3362166"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VI935_01525"
     CDS             <1..214
                     /locus_tag="VI935_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24313.1"
                     /translation="IRGDCLQCGGKLHATVTKGIVEKYFKLAMKLCNEFNVDQQTKTR
                     LEIIANNLKMLFKTEPVQSDLTGFLE"
     gene            complement(184..1029)
                     /locus_tag="VI935_01530"
     CDS             complement(184..1029)
                     /locus_tag="VI935_01530"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HLE24314.1"
                     /translation="MVQAEKPLSGKRILITRPRKQSKEISNEIKLFGGIPVLLPLIKI
                     RTLFDENEFNKFLAYLSESEIDYVVFMSVNSVKSLLSNARKFAKVSVLVKALRKSLIV
                     AVGERTATYLGRVNIHVDIIPKKFSSEGVVRELLNRNVRNKIIYIPKTNSSNNHIKKQ
                     LEKSGAIIHEYYVYETVIPKSLPLLQDVVKQLLERKIWAITFMSPSTVRSFLGLAGRL
                     LNRRNLISSLNQIVIAAIGSTTKNELEGEGILVDVVPAKHTSHELVLGLVKYAIYSKK
                     PVKSD"
     gene            complement(1005..1778)
                     /gene="cobA"
                     /locus_tag="VI935_01535"
     CDS             complement(1005..1778)
                     /gene="cobA"
                     /locus_tag="VI935_01535"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HLE24315.1"
                     /translation="MNGVKQKLIGKVYLVGAGPGDPGLLTLKGLEVLKKADVVIYDRL
                     VSSEVISLIPKKSKKIYSGKGAGDAEKLQRRINRIMVEEAKTGKDVVRLKGGDPFVFG
                     RGGEETQYLKKRGVVFEVVPGITSAFAAPAYAGIPVTHRNFASSVAVITGTEGIDGRT
                     SVNWRKIGSSIDTIVVLMGVSNLQKIIRYISSSGKSLRTDVAIIEWGTTRRQRVVSGT
                     LSNIIEKARKSNVKAPAVIIVGKVVSLGRELAWFKPKNR"
     gene            complement(1747..2679)
                     /gene="hemC"
                     /locus_tag="VI935_01540"
     CDS             complement(1747..2679)
                     /gene="hemC"
                     /locus_tag="VI935_01540"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HLE24316.1"
                     /translation="MIVKIGTRGSRLSLIQTDSVIDMLRRIGSDLEFQKVIMKTAGDV
                     FREKSLYSFGAKGIFEREINLAVIEGRVDFAVHSLKDLPSEEYDELGLAAIPKRESPN
                     DVLISNSRKTFTELKANNIVGTSSVRRMMQIKLHRPDLRVVRLRGNVETRLRKAANGE
                     FDAVVLAEAGVKRLGLQDKVSERLPLNMFVPAPGQGALVAVARKDDDKMLRLLSKIDD
                     RESRNECVVERIVASKLSGGCQVPMGIIAHQTGNSLSAYSALYSIDGRKKIACNIEGK
                     SDNASDVAERLANKLLEFGASGIIDEWRKTKVNW"
     gene            complement(2676..3947)
                     /gene="hemL"
                     /locus_tag="VI935_01545"
     CDS             complement(2676..3947)
                     /gene="hemL"
                     /locus_tag="VI935_01545"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HLE24317.1"
                     /translation="MNSEKLYKKALKLFPGGVNSPVRVFPPYPFFTKNGKGSKLTSVD
                     GRKYIDYCMSYGALLFGHAYPPIINAIKKSVRYGTIFGTPSEKEIELAELIIGSIPSI
                     EMVRFVNTGAEATMHSIRLARGFTNRSKIVKFEGCYHGAHDSVLVKAGSGATSFGVPD
                     SLGVTNDVAENTIVLPYNNSEKFESVIEEKHGEIAAVIVEPVIGNAGLIIPKDDFLQK
                     LRSMTRRYGIVLIFDEVITGFRLGLGGAQEYFGVKADLVTYGKVIGGGLPFAFFGGNR
                     EIMEMLAPEGKVYQAGTYSGNPLCVSVALVVLNELKRNHTRVYSKINGSCKSLSGALN
                     DIIRDRKVEAKVYSIASMFQIFFTRNNVIDYVSALTSNITLFRRYFAGILHAGIFVPP
                     SQFETCFISTTHTEDDIDKTVNVIDLTMSKL"
     gene            complement(3944..4927)
                     /gene="hemB"
                     /locus_tag="VI935_01550"
     CDS             complement(3944..4927)
                     /gene="hemB"
                     /locus_tag="VI935_01550"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HLE24318.1"
                     /translation="MSFPKVRMRRLRKNSAVRRLLGGTSLSVSNFVYPIFVDENIINK
                     QPIKSMPSQYRYSLKTLVHEVKEIVSLGIPAVILFGIPKVKNDTGSVAYARNGIIQKA
                     VRLLKSNFGNTLAVITDVCLCEYTTHGHCGILKENDVANDETLNALVKIAVSHVEAGA
                     DIVAPSSMMDGQVSRIRDGLDKDGFSDVSILAYSAKFASSFYRPFREAVDSKPAFGDR
                     KSYQMSYTNAQESLREVELDITEGADIVMVKPALVYLDVIKVVKGKFSIPLAAYNVSG
                     EYSMIKNASRIGLIDEKEAVLEILTAIKRAGADIIVTYHAKDVAKWLNEGI"
     gene            complement(4943..6199)
                     /gene="hemA"
                     /locus_tag="VI935_01555"
     CDS             complement(4943..6199)
                     /gene="hemA"
                     /locus_tag="VI935_01555"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HLE24319.1"
                     /translation="MDKAFTLSIINLRVTHKKVTIPILEAVRLKGDSISSIAQETIII
                     QTCNRVEIYVAVNEEQKEKTQRLLIQAWKKRSKKRVLNNIGKDFEDFLEIDYDADAIR
                     HLFRLVAGLESMIVGEDQILGQVRNSLVQVRLSKNIGPVLSLIFDRAIKLGAKVRTET
                     KINKNAVSIGSIAVNLAENVLGDLKNKSSLLIGAGEVGLLVAKSLVSKAQSTMFVTSR
                     SLNRARSLTSVVGGKALKFDDALKKLDSVDFVVLATSAPYHILTKQRIEQVNGKRNGK
                     LLFVLDLSNPRNVEESVADLPNVKLMTIDSIRDEIDHNLAARVNEIKHVEKRIENELV
                     KVFATLRREHVKPVIASFYKHAESIRVRELNRALQLLGDVDFGTLRIINDLSLALVEG
                     ILNEPVSNLRRVAENYDMKLAELIEI"
     gene            complement(6192..6851)
                     /locus_tag="VI935_01560"
     CDS             complement(6192..6851)
                     /locus_tag="VI935_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HLE24320.1"
                     /translation="MKREVFAVLIDLYLNGKQVVVFGGGSEAELKIVKALDGGAKITV
                     VSKDFTQGIMGLAHAGKVRVIEYDLKDGYSALKKQIGKPYVVFLATDDFKLNQRGVEF
                     AKGLGAIVCVVDTPELCDFAMPAIAKIGDIRIGISTGGRSPIVAKLIRQRIEGLITDE
                     DLVQLELQEYARRKIRSRMNAFPLRKKFLWKIIEDDEIQNLVKARKLEQAKLLVDRRV
                     G"
     gene            complement(6848..>7291)
                     /locus_tag="VI935_01565"
     CDS             complement(6848..>7291)
                     /locus_tag="VI935_01565"
                     /inference="COORDINATES: protein motif:HMM:NF037131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HLE24321.1"
                     /translation="KIIELQKDIELVSRPFLNMSKRLAMTEEQIINHVKMFEEIGIMR
                     RFAAILRHQKAGFVANGMVTWKVPSERIQEVANVAISFSQVSHCYERPVYEDWPYNLF
                     SMVHARSKEACERIALEISKRTGITDYVILYSTKEYKKDRVKYFV"
BASE COUNT         2234 a         1724 c         1029 g         2304 t
ORIGIN      
        1 tatcagagga gactgtcttc aatgcggggg gaaactacac gctactgtca ctaaaggcat
       61 agtagaaaaa tactttaaac tggctatgaa actgtgcaat gaattcaacg tagatcagca
      121 aactaaaact agattagaaa tcatagctaa taacttgaaa atgcttttta aaacagaacc
      181 cgttcaatca gatttgacag gtttcttgga gtaaatagca tatttcacta aacccaaaac
      241 caattcatgt gaagtatgtt ttgcaggaac cacatcaact aatataccct ccccctcaag
      301 ttcattttta gttgtcgaac caatagcggc aattacaatt tggttaagac tagaaattaa
      361 atttcgtctg tttaataaac gacctgctaa acccaaaaaa ctccttactg tggaaggact
      421 cataaatgtt atagcccaaa ttttacgctc taaaagctgt ttaacaacat cttgtaacaa
      481 aggcaaactc tttggaatta ctgtttcata cacgtaatac tcatgtataa tagcaccaga
      541 tttttctaat tgtttcttga tgtgattatt tgaagaattg gttttaggaa tgtaaattat
      601 cttattccga acattcctat ttaaaagctc cctaacaact ccttcagaac taaatttttt
      661 cggaataata tcaacatgaa tgtttacacg tcccaggtac gtagcggttc tttcaccaac
      721 agcgacaatg agagacttcc ttaatgcttt gaccaaaacc gaaactttgg caaattttct
      781 tgcatttgaa agaagagatt taacactatt aacgctcata aaaaccacat aatctatttc
      841 actctcagaa agataggcga ggaatttatt gaattcattt tcatcaaata acgtcctaat
      901 tttgattaat ggaagaagta cgggtatccc accaaacagc ttaatttcat tagaaatctc
      961 tttagactgt ttccttggac gcgtaattag gattctttta ccactcaacg gtttttcggc
     1021 ttgaaccatg ccagctctct tcccaatgaa acaaccttcc ctacaataat cactgcagga
     1081 gctttaacat ttgacttcct tgccttctct ataatattac tcagagtccc tgaaaccact
     1141 ctttgccttc tagtagtccc ccattctatt attgctacat ctgtcctgag acttttacca
     1201 gaactggaaa tatatctgat tattttctga agattagaca cacccattaa tacaacaatc
     1261 gtatcaatag aagaaccaat ctttctccaa ttgacactag ttcttccatc gatcccttca
     1321 gttccagtaa ttacagctac agatgatgca aagtttctat gagtaactgg tataccagcg
     1381 taagctggtg ctgcaaaagc agaagtaatc cccggtacca cttcgaatac aacgccgcgt
     1441 ttcttaagat attgggtttc ctctccgcct cgaccaaata caaacggatc tccacctttt
     1501 aatcgaacaa catctttacc agtttttgct tcctcaacca taatgcggtt tattcgccgc
     1561 tgaagttttt cagcatcacc agctccttta ccagaataga tttttttgga tttttttgga
     1621 attaagctaa taacttcact actaacaagt ctatcgtata taacaacatc agcttttttt
     1681 agaacttcta agcctttgag tgttaaaagt cctgggtctc ctggtcctgc accaaccaag
     1741 taaaccttac caattaactt ttgttttacg ccattcatct ataataccag acgctccaaa
     1801 ttcgagtaat ttatttgcaa gcctttcagc aacatcggaa gcgttatccg attttccttc
     1861 aatattacaa gctattttct ttcgaccgtc tatcgaatat aaagcagaat aagccgataa
     1921 actattacct gtctgatgag caataatacc cattggtact tggcaacctc cactcagttt
     1981 tgaagccaca atcctttcta caacacattc atttcttgat tccctatcat ctatcttcga
     2041 tagtaatctc aacattttat catcgtcttt cctagctact gcaactaacg caccttgccc
     2101 gggtgctggt acgaacatat tcaaagggag tctctcggaa actttgtcct gtaatcctaa
     2161 cctcttcaca cctgcttcag caagaacaac agcatcaaat tccccatttg cagctttacg
     2221 gagtcttgtc tcaacattcc ctctaagcct aacaaccctc aaatcaggcc tgtgaagttt
     2281 aatctgcatc attcttctaa cgctgctagt tcccacaata ttatttgctt tgagttcggt
     2341 aaacgtcttg cgactattcg aaattagcac atcgttggga ctttctcttt ttggaattgc
     2401 agctaaacct aattcatcat actcttctga aggtaggtct ttaaggctat ggactgcaaa
     2461 atccactcta ccttcaatta ctgctagatt aatttctctt tcaaatatac ccttagcacc
     2521 aaacgaataa agagactttt ccctgaacac atctccggca gtcttcatga taactttctg
     2581 gaattccaaa tcagaaccaa ttcttctgag catgtctatg acagaatctg tttgaataag
     2641 agaaagccta ctccctctag ttccgatttt aacaatcata atttcgacat cgtcaaatca
     2701 ataacattca cagttttatc aatatcatct tctgtatgtg tagtagaaat aaaacaagtc
     2761 tcaaattgtg agggaggaac aaatatacca gcgtgcaaaa tacctgcaaa gtacctgcga
     2821 aacagagtaa tgttagatgt taaagccgaa acataatcaa taacattatt tcgagtaaag
     2881 aagatctgaa acattgatgc tatagaatac accttggcct ccaccttcct atccctaata
     2941 atatcattta acgctccgct caaactctta caactcccat taatcttaga atatactcga
     3001 gtgtgatttc tttttaactc atttaacaca actagagcta cagatacaca taatggattt
     3061 ccactataag ttccggcttg atagacctta ccctctgggg ctaacatttc cataatttct
     3121 ctgtttcctc caaaaaatgc aaatggcaac cctcctccaa tcaccttccc atacgtaaca
     3181 agatctgcct tcactccgaa atattcctgc gctccgccca agcccaacct aaaaccagta
     3241 ataacttcat caaaaattaa gacgattcca tatcgtcttg tcatactcct caatttctgc
     3301 aaaaaatcgt ctttaggtat gataagccca gcattcccaa ttacaggttc gacgataact
     3361 gcggcaattt caccatgttt ctcttcaata acactctcaa atttctcgga attattatag
     3421 ggaagtacaa tggtgttttc tgcaacatca tttgtgacac caagagagtc tggaacacca
     3481 aaactcgtag caccggaacc ggcttttaca agtacagaat catgcgcacc atgataacag
     3541 ccttcaaatt ttacaatctt acttctatta gtaaaccccc tagcaagtct tattgagtgc
     3601 atagttgctt cagcaccagt attcacaaac ctgaccatct caatcgaagg aatactacca
     3661 attatcaact cagcaagttc aatttctttc tctgacggcg taccaaagat cgttccatac
     3721 ctaacagatt tttttatagc atttattatc ggaggataag cgtgaccaaa caacaaagct
     3781 ccatacgaca tacaataatc tatgtattta cgaccgtcaa cactagttag tttcgaacct
     3841 ttcccatttt tcgtgaaaaa cgggtaagga gggaaaaccc gcacagggct attgacacca
     3901 ccaggaaaca gttttagagc tttcttgtag agcttttctg aattcaaata ccttcattca
     3961 accatttagc tacatctttt gcatggtatg ttacaattat gtctgctcca gcacgtttaa
     4021 tcgccgtcaa tatctctaac acagcctctt tttcgtcaat cagaccaatt ctggatgcat
     4081 tttttatcat agaatactct ccactgacat tataggctgc tagaggaata gaaaacttac
     4141 ctttcactac ctttatcaca tcaaggtaaa caagtgcagg ctttaccata actatatcag
     4201 ccccttcagt aatatcgagt tcaacctctc ttaatgattc ctgtgcatta gtataactca
     4261 tctggtagct ttttctatca ccaaacgcag gtttcgaatc aaccgcttcc ctgaaaggtc
     4321 tgtaaaagga cgacgcaaat tttgcagaat aggcaagaat agaaacatcc gagaaaccat
     4381 ctttatccaa accatctctt attctagaaa cctgcccatc catcatgctt gatggtgcaa
     4441 cgatatctgc accagcctcc acatggctaa ccgcaatttt tacaagcgca ttaagggtct
     4501 catcatttgc aacatcattc tcctttaata taccacaatg accatgtgtc gtatactcac
     4561 atagacaaac atctgtaatc accgcaaggg tattaccaaa attcgatttc agtagtctca
     4621 cagccttctg aatgatgcca tttcttgcat acgccacaga tccagtatca ttcttaactt
     4681 taggtatccc aaacagaatt acagcgggaa tacctaaaga aacaatctcc ttaacttcat
     4741 gaacaagcgt ctttagtgaa tacctgtatt gagaaggcat agattttatt ggctgcttat
     4801 taataatatt ttcatccaca aatattggat aaacaaaatt ggatacactt aacgaagttc
     4861 cgcccaataa ccttctcaca gcagaatttt ttctgagcct tctcatcctt accttcggga
     4921 agctcaacac tctcactttg ccttaaatct cgattagttc ggccaatttc atatcataat
     4981 tttcagcaac cctacggaga ttcgaaacag gttcatttag aattccttct actaaagcaa
     5041 gactcaaatc attaatgata cgtaacgttc cgaaatctac gtcaccaagt aactgcaaag
     5101 ccctattcaa ttcacgaact ctaatagatt cagcatgctt gtagaacgag gctataacag
     5161 gtttcacatg ctctcttctt aacgtagcaa acacctttac caactcattt tcaattcttt
     5221 tttcaacatg cttgatttca ttaacacgtg cagctaaatt gtgatcgatt tcatctctga
     5281 tactatctat ggtcattaat tttacattgg gtaaatcagc aactgactcc tcaacatttc
     5341 tcggattcga gagatctaaa acaaataaca attttccatt tctttttcca tttacttgtt
     5401 caattcgctg ttttgttaga atgtggtatg gtgcagaggt agccagaaca acaaaatcaa
     5461 cactatcaag tttctttaac gcatcatcaa attttaatgc ttttccacca acaacactag
     5521 taagtgatct agctctgttc aaagatctac tcgtaacaaa catcgtactc tgagctttag
     5581 atactaaaga ctttgctaca agtaaaccaa cctccccagc acctattaac aacgaagatt
     5641 tgttcttcaa atctcctaga acattttcag caagatttac tgcaatcgag cctatcgaaa
     5701 cagcattctt gttaatctta gtttcagtcc taacttttgc tcctagcttt atagctctat
     5761 caaatatcag cgaaagcact gggcctatgt ttttagaaag tctcacttga actaaggaat
     5821 ttctaacttg accgagtatt tgatcttcac caacaatcat tgattcaaga cctgcaacta
     5881 gcctgaaaag atgccttatc gcgtctgcat cataatctat ttccaaaaaa tcctcaaaat
     5941 cctttccaat attatttaaa acacgtttct tggacctttt tttccaagct tgtattaaca
     6001 aacgctgggt tttttctttt tgttcttcat taactgcaac ataaatctca actctattgc
     6061 aagtttgaat aataatagtt tcctgagcaa tagaagatat cgaatcccct ttcaatctaa
     6121 ccgcttcaag tattggaatc gttacctttt tgtgtgtgac acgtaaatta attattgaca
     6181 aagtaaacgc cttatccaac tcttcggtct acaagtaact tcgcctgctc taatttcctt
     6241 gcttttacta gattttgaat ttcatcatct tcaattattt tccatagaaa tttctttctt
     6301 aatgggaatg cattcatcct agaccttatc tttcttctcg cgtattcttg caactccaat
     6361 tgaaccaaat cctcatcagt aatcaaacct tctatacgtt gtctgatcaa tttcgcaact
     6421 attggacttc tacctcctgt cgaaattccg attcttatat caccgatctt agctatagcg
     6481 ggcatggcga agtcacataa ttcaggagta tcgacaacac atacaatagc acctaaaccc
     6541 ttcgcaaatt ccacacctct ctgattcagt ttaaaatcat ccgtagctag gaaaaccaca
     6601 tatggctttc ctatctgttt ctttaaagca gaataaccat ccttcaaatc atattcaata
     6661 accctaactt taccggcgtg agcaagtccc attattccct gtgtgaaatc tttgctaact
     6721 actgtaatct tggcgccacc atcaagtgct ttaacaatct tcaactcagc ttcactccct
     6781 cctccaaata cgacgacttg cttaccgttg agataaagat ctataagcac tgcaaaaacc
     6841 tcacgtttca cacgaaatat ttgactctgt ccttcttgta ctctttagtg ctatataata
     6901 ttacataatc cgttattcct gtcctttttg atatctccaa ggctattctt tcacaagcct
     6961 ccttgctacg tgcatgtacc attgaaaaga ggttataagg ccagtcttca tacactggcc
     7021 tttcataaca gtgacttact tgcgagaatg agatcgcaac gttggcaact tcttgtattc
     7081 tctctgaagg aactttccat gttaccatac cgtttgccac aaaccctgct ttttggtgtc
     7141 ttagtatagc agcaaacctt cgcataatac caatttcttc aaacatcttt acgtgattaa
     7201 ttatctgctc ttctgtcata gctaaccgtt ttgacatatt caagaaaggc cttgacacca
     7261 attcaatgtc tttctgcaac tcaataattt t
//
LOCUS       DATDRL010000042         4858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_731992, whole genome shotgun sequence.
ACCESSION   DATDRL010000042 DATDRL010000000
VERSION     DATDRL010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731992"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VI935_01570"
     CDS             <1..394
                     /locus_tag="VI935_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006458384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLE24322.1"
                     /translation="IERLQELDPDLARYILEFPFGDIYNRPGLDLKSREIATLAALVA
                     LGNAIPQLKIHIHGALNVGVSRQEIIEIIIQMAVYAGFPAALNAMYAAKEVFIEIDEK
                     AKKKENVKLKDFKPEKSPPHLIKQNEQS"
     gene            381..1310
                     /locus_tag="VI935_01575"
     CDS             381..1310
                     /locus_tag="VI935_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLE24323.1"
                     /translation="MNNHKSGKVKVNDVNIYHEIHGEGKPLLLIEGLGYSSWMWFKQI
                     PALSRKYKVIVFDNRGVGNTDKPDREYTMEIMADDAAGLLKALGIDSAHVLGVSMGGF
                     IAQELALKYPDMVRGLVLVSTSFGGEGSMPRGSNLWNTFVKLWGLMPDVIEFSNKGNV
                     AMPLINSLCLTPKEKIRYGLSLAFTPEYFINHPGEIDRIVERRLADKQPSYAWKHQLI
                     AGMNFYAADRVHRIKAPTLVITGSEDRVVPPERSKRLAEEVPNSRFITFEETGHLLFI
                     ERAEEFNEIVLSFLQQLSKDKEPEIEWKEREKR"
     gene            complement(1748..2923)
                     /gene="bioF"
                     /locus_tag="VI935_01580"
     CDS             complement(1748..2923)
                     /gene="bioF"
                     /locus_tag="VI935_01580"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HLE24324.1"
                     /translation="MRNPLHWIEEELKLICDKNLFRVLTELQSGQSPEIIIEGKRYIL
                     LASNNYLGLTTDPKVKEAARRALEKYGTGSGGSRLVSGSTDLHRKLEERIAAFKKTEA
                     AILFSSGYLANVGTISALVRPGDVIYSDELNHASIIDGCRLSHAEIKIYKHCDVEHLN
                     SLLGSNKSKNKELIVTDTVFSMDGDLAPLPELIKLAEKYGCMLMIDEAHATGVLGKRG
                     SGATEYFGVEDKVSIVMGTLSKAVGSLGGYIAGSQKLIDFVRNTVRSYIFDTSLPASS
                     LAASLAAIDIIELEPERRKHLWHLIKSFKSGLKNIGLELLPSHSAVIPVLIGEAQLAL
                     DFARQLRKNGVFTPAVRPPSVPPGRCRIRATLMATHKEEHIELALRAFKAARESVLK"
     gene            complement(2990..4345)
                     /gene="bioA"
                     /locus_tag="VI935_01585"
     CDS             complement(2990..4345)
                     /gene="bioA"
                     /locus_tag="VI935_01585"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HLE24325.1"
                     /translation="MKGRTKKLGIYDREFVWHPFTQMREYEKEEPIVIEEGNGIYLVD
                     TEGRRYIDGVSSLWVNIHGHKVPEIDKAIKDQIDKIAHTTLLGITNPTAVELAKELIN
                     ICPPGLKKVFYSGDGASSVEVALKMAFQYWQHRGKPKKTKFVSLENGYHGDTLGAVSV
                     GGIDLFHATFKPLLFETYKAPSYYCYRCPLGKTYPMCELACAKEVEKILEQHHEEIAA
                     VIFEPYVQAAGGMIVSPQGYIKRVREYCDKYDVLMILDEVATGFGRTGKMFACEYEDI
                     TPDIMVLGKGMTGGYLPLSATITTQEVYDAFLGEYEEFKTFFHGHSYAGNPISCAAAL
                     GNLEVFKKYNTLEKLQEKVEFLEQELKEFKGLKHVGDVRNKGLMVGIELVEDKETKKP
                     YELKMKMGWKVADFAKEEEVLIRPLGNVVVLMPPIGISAKDLKKLLDVTYRAIRKATE
                     S"
     gene            complement(4453..>4858)
                     /locus_tag="VI935_01590"
     CDS             complement(4453..>4858)
                     /locus_tag="VI935_01590"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894481.1"
                     /note="catalyzes the formation of biotin from dethiobiotin
                     and sulfur 2 S-adenosyl-L-methionine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HLE24326.1"
                     /translation="VKKAGMNLCSGGILGMGESVRQRIELAFEIRELDPSWVPINFLN
                     PRPGTPFANLPLVSPFEAVKTIAIFRLILPDKILMTAGGREVTLRDLQAMGLLAGANA
                     MILGNYLTTPGRPPEEDLRMLDDLQMPVRKDV"
BASE COUNT         1423 a         1081 c          936 g         1418 t
ORIGIN      
        1 cattgagcga ctacaagagc ttgaccctga cctagctcgc tatatactcg aattcccatt
       61 cggagatatt tacaatcgtc caggattaga tttgaaatca agggagattg ctacccttgc
      121 tgcgctcgta gcgttaggta atgccattcc acaattgaag atacatattc acggagcgct
      181 taacgtcggg gtatcccgcc aagagattat cgaaataatc attcaaatgg cagtttatgc
      241 aggttttcct gcagcgctta acgctatgta cgcggcaaaa gaagttttta ttgaaataga
      301 cgaaaaagca aagaagaaag aaaacgtgaa acttaaagat tttaaaccag aaaaatcacc
      361 accccattta attaaacaaa atgaacaatc ataagtccgg caaagtaaaa gtaaacgacg
      421 tcaatatata ccatgaaatc catggagaag gtaagccgct tcttcttatc gaggggctcg
      481 gctactcaag ttggatgtgg tttaaacaga ttcctgcttt atccagaaaa tataaagtta
      541 ttgtttttga taatcgtgga gttggaaata ccgataagcc cgacagagag tatacgatgg
      601 agataatggc agatgatgca gcaggtcttc tcaaggctct tgggatagac tcagctcatg
      661 ttcttggggt ctctatgggc ggcttcattg cccaagagct ggctctaaag tatccagata
      721 tggtaagagg tcttgtttta gtctcaacga gctttggagg agaaggtagt atgcctaggg
      781 gttccaatct ctggaacacc tttgtaaaac tatggggatt aatgcctgat gtgatcgaat
      841 ttagtaataa aggcaatgtc gctatgcctt tgattaactc cctttgctta acgcctaaag
      901 aaaagattcg ttacgggctt tcattggctt tcacacctga atactttatt aaccatcctg
      961 gggaaattga ccgcattgta gagcggaggc ttgccgacaa acaaccatca tatgcatgga
     1021 aacaccaact tattgccggg atgaactttt acgcggctga tagggtgcat cgaataaaag
     1081 ccccaacact tgtgataact ggatctgagg atagagtcgt accacctgaa agatcaaagc
     1141 ggcttgcaga agaggttccg aattcaagat ttattacttt tgaagaaaca gggcatctcc
     1201 tctttataga gcgtgctgag gagtttaatg agattgtact tagctttttg cagcaactaa
     1261 gtaaagacaa ggagcctgag atagagtgga aggaaaggga aaaacggtga aagagaatct
     1321 tttctttctc cctccacaat aagaaaccaa ctttttacaa tcgcaaagat agtaagaaga
     1381 atatttcagc aggttatact tgttcctcac catcccaata atcaatttca gagtcacttc
     1441 gtaggtattt atgcacggta cgcttttcct taagcccgcc aggtgcagga cccacaaaga
     1501 taccaatctt attataggcc ggatacacta tcacgtctgg ttctatcata gtggagaaag
     1561 ataagaacag cagtgtacta tccgaagtat taatgattta tggttcataa tctccgccta
     1621 tatcgagtat agaacagaca agaatgtctg ttctaccaga tacccataga tatgcacaaa
     1681 tggttggtgg gtcagacatt cctgtctaat ttttggaaag aatctattcc attacgtcaa
     1741 actcacgtta ctttagaact gattcgcgtg ccgctttaaa cgcacgtagc gctaactcaa
     1801 tatgctcctc tttgtgtgtt gccataagcg ttgctctaat tcgacatctt cctggtggaa
     1861 ctgaaggtgg gcgaacagca ggtgtaaaaa ccccattttt acgaagctgt ctggcaaaat
     1921 ctaacgctag ctgcgcttca ccaatcagaa ccggaataac agcagaatgt gaaggaagca
     1981 attccagtcc aatatttttc agtcctgatt taaaactttt aatgagatgc catagatgct
     2041 ttcttctctc aggttcaagc tcgataatat caattgcagc caaagaggca gcaagggagc
     2101 ttgcaggaag tgaggtatcg aagatatagc ttctcaccgt gtttctcaca aagtcaatta
     2161 gtttttgact cccagcaata tatccaccca gagagccaac tgcttttgaa agtgttccca
     2221 taactatact aaccttgtct tctacaccaa agtattccgt ggcgccgctt cctctttttc
     2281 ccaaaactcc tgttgcatgc gcttcatcaa tcatcagcat gcagccatat ttttcggcaa
     2341 gtttaattaa ctcaggaaga ggtgctaaat ccccatccat actaaaaact gtatctgtca
     2401 caatgagttc tttattcttg gacttatttg agccaagaag agagtttaaa tgctccacat
     2461 cacaatgctt atatatcttt atttcagcat gggaaagtcg gcatccgtca atgattgatg
     2521 cgtgattaag ctcgtcactg tagattacat ctcctggtct gaccagagca gagatagtac
     2581 ctacatttgc aagataacca gaactaaata gtatagccgc ctcggttttc ttaaaagctg
     2641 caattctttc ctctaatttt ctatgaagat ctgtacttcc gcttacaaga cgtgaaccac
     2701 ctgaacctgt accgtacttt tctaaagccc ttcgtgcggc ttctttcacc tttggatcag
     2761 tcgtaagacc taggtagtta tttgaagcta gtagaatata acgcttacct tcaataataa
     2821 tttccggtga ctgcccgctt tggagttctg taaggactcg aaacaggttc ttatcacaaa
     2881 ttagttttaa ttcttcctca atccaatgaa gtgggtttct catgtttata gtgaaccgtg
     2941 actcgcggct tgtgaatcgt ggatcgaatc acgacccacg aaactcggat cacgattccg
     3001 tcgctttcct gatcgcccta taagtcacat caaggagttt ttttaaatcc ttcgctgaaa
     3061 tcccaatagg aggcataaga acaaccacgt ttccaagagg acgtattaga acctcttcct
     3121 ctttcgcaaa gtcagcaacc ttccaaccca ttttcatctt aagctcatat ggtttttttg
     3181 tttctttatc ctctacaagc tcaataccta ccattaatcc tttgttccta acatcaccta
     3241 catgctttag acctttaaat tccttaagtt cctgttctaa gaactctacc ttttcctgca
     3301 atttttcaag agtattatac ttcttaaaaa cctcaaggtt tcctaaagcc gctgcgcacg
     3361 aaatcgggtt tccagcatag ctatgaccat gaaagaatgt tttaaactcc tcatattcac
     3421 cgaggaaggc gtcgtagact tcttgagtcg taatcgtagc agaaaggggg agataaccgc
     3481 ctgtcatccc ttttccaaga accattatat cgggtgttat gtcttcatat tcacaggcaa
     3541 acatcttacc tgttcttcca aaccccgtag caacctcatc tagaatcata agcacatcat
     3601 atttatcgca atattctctt actctcttaa tataaccctg aggagaaaca atcataccac
     3661 ctgccgcttg aacgtaaggt tcaaatatta ccgctgcaat ctcttcatgg tgttgctcaa
     3721 gtattttctc gacctctttc gcacaagcga gctcacacat gggataagtc tttccaagag
     3781 ggcatcggta gcaataatac gatggggctt tatatgtttc aaaaagaagg ggtttaaaag
     3841 tagcgtgaaa taaatcaatt ccacctacag aaacggcgcc aagcgtatct ccatgatagc
     3901 cgttttctaa agaaacaaat ttcgtcttct ttggttttcc tctgtgctgc cagtattgaa
     3961 aagccatctt aagcgcaacc tcaactgagc ttgcaccatc acctgaataa aaaaccttct
     4021 ttagccctgg aggacaaata ttaatcagct cctttgcaag ctctactgct gtagggtttg
     4081 ttatgccaag aagggtggta tgagcaattt tgtctatctg gtctttaatt gccttatcaa
     4141 tttcaggaac tttatggcca tgaatattaa cccagagtga agatacgcca tctatgtatc
     4201 ttcgcccttc tgtatcaaca agataaattc cattgccttc ttcaataaca atcggctcct
     4261 ctttttcata ttccctcatc tgggtgaagg gatgccaaac gaattcccta tcgtaaatac
     4321 ctagtttttt tgtccggcct ttcatgttat tcagaaaata aagagagagt caccctttcg
     4381 tgaatcgtgg ctcgtgacta gtggttcgca attttataaa cgattcacga aacccgaatc
     4441 acatgtcacg aactaaacat cttttctcac aggcatttgg aggtcgtcta gcatccgtaa
     4501 atcctcctca ggaggacggc caggcgttgt aagatagttg ccaaggatca tagcatttgc
     4561 accagctaag agtcccattg cctgaagatc gcgaagggta acttctcttc cacctgcagt
     4621 cataagaatc ttatccggta gtattaatct gaatattgca attgtcttta ctgcctcaaa
     4681 cggtgatact aaaggtaaat tggcaaaagg tgtaccgggt cttgggttaa gaaagttaat
     4741 tggtacccaa cttggatcca gttctctaat ttcaaaagca agttcaatcc tctgccttac
     4801 agattcaccc atacccaaaa taccacctga gcaaaggttc ataccagcct tttttact
//
LOCUS       DATDRL010000043         3419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_460853, whole genome shotgun sequence.
ACCESSION   DATDRL010000043 DATDRL010000000
VERSION     DATDRL010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460853"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="VI935_01595"
     CDS             complement(<1..1130)
                     /locus_tag="VI935_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007354777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLE24327.1"
                     /translation="MQNTLEKPKKVVIIGAGPAGLTAAYELCKAGIESLVLEKDNIVG
                     GLARTVKHNGCHFDMGGHRFFTKVKVVEEMWHEVLGEDFLRRNRLSRIYYNKRYFYYP
                     LRALNALSGLGVWNSFLILLSYFKSRILPDTPEETFEQWVSNRFGRRLYNTFFKTYTE
                     KVWGIPCSEIRAEWAAQRIKGLSLLTALKNALLDQQNSHKGAIIKTLIDSFHYPKFGP
                     GMMWEKVANLVQNDGSEVRLGTEVERIFWTSDKIDSLEVKSNGQKELVKGTHFISTMP
                     IRELIQNFEPSVSENVLNAATVLNYRDFLTVVLIINKRDVFPDNWIYIHDPKAKVGRI
                     QNFKNWSPYMVPDQDKTCLGLEYFCFEGDGLWTMSNQELIELG"
     gene            1626..2693
                     /locus_tag="VI935_01600"
     CDS             1626..2693
                     /locus_tag="VI935_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24328.1"
                     /translation="MNKEGQKENSLVSEELRNESKEPVATGDVFNWNTLNLGKQEDKF
                     NIETPDTKGTEDKSGISNKADYSGFKWENIRIDNEPIDSLLDPPKLFDEVKEVDNTQQ
                     KGDNLSQENELSHVDTPKPKQGKLVVDNERLLITQRVSSKAYSSYMPKVRPKELGSKI
                     SLSKKIITFFVSLFILLIITSSSVWIMITLELIPKDKADRLTTFTLSKLPIRIFERLD
                     DSVVMSQDSGRWLSTRNGFIYVVSGSIENKSKYDVSYIKIKGEFLSGGKKIFEQVVYA
                     GNTFSETELKTLPFEYILNKLQRKNGDINFNYPNKLAGLNYDIKPDETVPFFIVFPSK
                     GKVLGLKYKTDIVGFEKANLK"
     gene            3228..>3419
                     /locus_tag="VI935_01605"
     CDS             3228..>3419
                     /locus_tag="VI935_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24329.1"
                     /translation="MKSTRFKISVSQDGLLPEETADVATLQFPILPKKLLKALGFERI
                     DTKTYVGVLPVFVNIGDKEQ"
BASE COUNT         1115 a          583 c          642 g         1079 t
ORIGIN      
        1 ccaagctcga ttaactcttg attagacatt gtccagagcc cgtcaccttc aaagcaaaaa
       61 tactctagtc ccaagcaggt tttgtcttga tctggaacca tataagggct ccagttctta
      121 aaattttgaa tccttcctac ctttgctttt ggatcatgta tataaatcca gttatcagga
      181 aatacgtcac gcttattaat aataagaacc acagttagaa agtctctata gttaagaacc
      241 gttgcagcgt ttaacacgtt ctcagacaca cttggttcaa aattttgaat gagctctcta
      301 atcggcatag tgcttatgaa gtgtgttcct ttaaccaact ctttttgacc attggatttt
      361 acttctaatg aatctatttt atcgcttgtc caaaaaattc tttcgacctc tgttcctaat
      421 cttacctcac tcccatcgtt ttgaacaaga ttagctacct tctcccacat catgcctggc
      481 ccgaacttag gataatggaa tgagtctatc agtgttttga tgatagctcc tttatgggag
      541 ttttgctgat ctaatagggc atttttaaga gcggttaata gtgaaagtcc ttttattcgt
      601 tgagcagccc attcagctct gatttcgctg catggtattc cccacacttt ttcggtatat
      661 gttttaaaaa aggtgttata aaggcgtctt ccaaatcggt tcgaaaccca ttgttcgaat
      721 gtttcttctg gcgtatctgg aagaattcta gacttaaagt aacttagaag aattagaaag
      781 ctattccaga ctcctaggcc agataaagcg ttaagggcgc gtaatggata ataaaaatac
      841 cttttattat aataaatacg tgacaaccgg ttacgcctaa gaaaatcttc acccaatacc
      901 tcatgccaca tttcttctac aaccttaacc ttggtaaaaa agcggtgtcc tcccatgtca
      961 aagtggcagc cattatgctt tacagtcctt gctaatccac caactatgtt atccttttct
     1021 aggactagag actcaatccc cgctttacat agttcatatg ctgccgttaa gcccgcaggc
     1081 ccagcaccta ttattactac tttttttggt ttttctaacg tgttttgcac ttaactctta
     1141 aataatgtgg attgttttaa cgatgtatct gttataagaa tgtagctccg ctttcttact
     1201 tacatcatag aagaataacc ccttgaagtc aacaagttta tctacttcat tgacaatcca
     1261 taaattgaat tgcaatattt taaaaatgaa atacaattaa acattgtata attcaacggt
     1321 tcaaaaaaag gaggaactat atgactgtaa caaggatatg ttgggaatgc ccggggtcac
     1381 ctgttcgtaa atggaacatc gtttctgaaa taaagggacg ggagggccac ctggttagta
     1441 ttagctgtcc aatttgccgc aaagaatcca gggtctttgg gtggatgatc ggttgtgaat
     1501 gtgattcatg taaatctcaa gttataggtg ctggcaaata attaatgtct tgttttttat
     1561 ttcaatccaa acattatact caaatgacga aaattctaat cagggattaa gaggctaata
     1621 cgcaattgaa taaggaaggc cagaaagaga attctttagt aagtgaagaa ttaaggaatg
     1681 agtcgaagga accagtagct acgggagatg tgttcaactg gaacacatta aatctcggaa
     1741 aacaagagga taaatttaat atagaaaccc ctgatactaa agggacagag gataagagcg
     1801 ggattagtaa taaagcagat tattcgggat ttaaatggga aaatatacgt attgataatg
     1861 aacctataga tagcctatta gatcccccaa agctgtttga tgaagtgaaa gaggtagata
     1921 atacacaaca aaagggtgat aatctaagtc aagaaaatga gttaagtcat gttgatacac
     1981 caaaaccaaa acaaggtaag cttgtagttg ataatgaaag gctattgata actcaaaggg
     2041 ttagctcaaa ggcatattcc tcatatatgc ctaaagtaag accaaaggaa ctgggttcaa
     2101 agataagctt atcaaagaag attattacat tctttgtttc cctttttatt ttattaatta
     2161 tcacctcctc ttcagtttgg attatgataa ctttggaatt aattcccaaa gacaaagctg
     2221 ataggttaac tacctttact ttatctaagc ttccaatcag gattttcgag cggctggatg
     2281 acagcgtagt catgtctcag gattcgggca ggtggttaag taccagaaac ggattcattt
     2341 acgttgtctc tggaagcatt gagaataaat ctaaatatga tgtaagttat ataaagataa
     2401 aaggcgaatt tttaagtggt gggaagaaga tttttgaaca ggttgtttat gctggcaaca
     2461 catttagcga aactgagcta aaaacccttc catttgaata tatactgaat aaacttcaga
     2521 gaaaaaatgg cgatattaat tttaactatc ctaacaaact tgcaggactt aattatgaca
     2581 ttaagcctga cgaaactgtg ccattcttta ttgtattccc atcgaagggc aaggtgctgg
     2641 gcttaaaata taagactgat attgtgggtt ttgagaaagc taatctgaaa tgatacctcc
     2701 cctttatatt aattgtagcg tccctaagta aaaccttcct tagaagtatt ttttaaggaa
     2761 gttttcgatg gtattagcaa tctaagtaaa tataagttgc acaatttaga gtttttgcat
     2821 gaaactattg ttttcttaat tttgaaaatt gtaatatgat taataatcta aatgaatttc
     2881 gaattatatc gcaaccgcaa cctctatcta ttaattataa actaattgtg gtttataaat
     2941 tacatataac aatatattgt ggttagaaat agttaatgcg gatctaggaa gcagtttaaa
     3001 cagtcttttt tacagaatgc ttgctatttt atataagtta agtaaaatac ttcattaata
     3061 aagaccgggt aagcgagggt aaagagatct cccctatttt ccttttagga agtctaaaca
     3121 acaataagag ggttgagatt aagctgaaaa ggatttaccg tgttactaat gcctggcttt
     3181 tagatataag gataagtgta ttttattaac aagggggaca acgcggcatg aaatctacac
     3241 gattcaaaat aagtgttagt caggatggtt tgcttccaga agaaacggca gatgtagcaa
     3301 cattacaatt tccgatccta cctaagaagc ttctaaaagc gcttggtttt gagaggattg
     3361 atacaaaaac ttatgttgga gtgcttcctg tttttgtgaa cataggtgat aaggagcag
//
LOCUS       DATDRL010000044         2931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_991628, whole genome shotgun sequence.
ACCESSION   DATDRL010000044 DATDRL010000000
VERSION     DATDRL010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991628"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VI935_01610"
     CDS             complement(<1..329)
                     /locus_tag="VI935_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HLE24330.1"
                     /translation="MSKKKIGIYVTALIIVILILAVLANRNSSTNNEVKVKKGNIDLI
                     VAAKGKVEPIEDVDLAPKTLGRLKEIYVGEGDFVKKDDVIAVLENEEIKAQVEQAKAN
                     ILRAEAEL"
     gene            complement(386..1108)
                     /locus_tag="VI935_01615"
     CDS             complement(386..1108)
                     /locus_tag="VI935_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLE24331.1"
                     /translation="MLTAFRKHTDLKTKQIEVIGIDKVYQEGSVRVHAVKGVTLSVDA
                     GELVLLMGPSGSGKTTLLSMMGCILKPTKGQVHIMGKEILYWDENSLPMIRRKYIGFI
                     FQHFNLLSALTACENVEASLNLKGIKGKVAELMSRELLKQVGLGDRCDFLPRDLSGGE
                     KQRVSIARALAGNPSIILADEPTGNLDSQNGRIAIELLKNLASKENKSVVVVTHDNRI
                     TDLADRIYHIEDGILKNGHPFL"
     gene            complement(1156..1458)
                     /locus_tag="VI935_01620"
     CDS             complement(1156..1458)
                     /locus_tag="VI935_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24332.1"
                     /translation="MKILQIVPKSGTKSKFKTLLKDKERDLRGRGTTFYREREGRWKH
                     ERYSGWISWDGAKGGLLVAEINTKRKESEWQLLQAFIGYLDRHLGEHIESISIYYR"
     gene            complement(1494..2621)
                     /locus_tag="VI935_01625"
     CDS             complement(1494..2621)
                     /locus_tag="VI935_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLE24333.1"
                     /translation="MMVSVARRNLFHDKTRLAITLVGIMFAVVLMTAQIGAYMAFVGN
                     ASQLIDNSPADIWITAKNTVNVDSAKPFSENKINKVRETKGVLWVEKIAQTWGLMKLK
                     SGGTESVQIIGFDTESGVGGPWKMKEGKIKDVKVGNSIIIDESSLKRLGAKLGDKVEI
                     FDKQTEIVGISQGVRSFTTYPIAFTSYKTAKKFSRIMDYDQTTFVLVKLEHGVNANEV
                     VEKLREKMSGVDVYTKEQFSRKTRMYWSIQTGMGLGVGITVFLGFIVGTVVVGQTIYS
                     STMEHIREFGTLKAIGATNLDIYKIILQQAIISASIGYALGLLFTLLTKNLYEYLGVV
                     LILTPKLLIAMFFITSIMCIISSFISIQKAAKVDPVIVFRA"
     gene            complement(2655..>2931)
                     /locus_tag="VI935_01630"
     CDS             complement(2655..>2931)
                     /locus_tag="VI935_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HLE24334.1"
                     /translation="DPRYYHDPFRFYPERWAPEQQASRPPFAYFPFGGGPRRCIGESF
                     AWMEGILVIATLAAKWKTSLVPGHRVELQPSITLRPKYGMRMTLERR"
BASE COUNT          794 a          681 c          521 g          935 t
ORIGIN      
        1 agctcggctt ctgcacgcag aatgtttgct tttgcttgct caacctgcgc ctttatttcc
       61 tcattttcga gcactgcaat tacatcatct ttcttaacga aatctccttc tccaacgtag
      121 atttccttta gtcttccaag ggtcttagga gcaaggtcaa catcctcaat tggttcaacc
      181 ttcccctttg cagcaacgat taaatctata ttgccttttt tgaccttaac ttcattattg
      241 gtagaggaat ttcggttcgc taatacagcc aatattagaa taactattat aagcgcagtt
      301 acatatattc ctattttctt tttactcatc gtatttatcg tattggtgcg ggtgtttaac
      361 aataattaac tttaatatca aacttttata aaaaagggtg tccatttttt aaaatcccgt
      421 cttcaatgtg atagatccta tctgcaaggt cggtgattct attatcatgt gtaacaacaa
      481 ctaccgattt gttttccttt gaagcaaggt tttttagaag ctctattgct atccttccat
      541 tctgggaatc aagattacca gtgggctcat cagctaaaat aatcgaaggg tttcctgcta
      601 gcgcacgcgc aatggaaacc ctctgtttct cccctccgct taaatctctc ggaagaaaat
      661 cacacctatc ccctagacca acctgcttta atagttctct agacataagt tccgcaactt
      721 tcccttttat accttttaaa tttaaagagg cctctacatt ttcgcaggca gtaagagcac
      781 ttaggaggtt gaagtgttga aagataaagc cgatgtactt tcgccttatc attggaagag
      841 agttttcatc ccaatacaat atctcttttc ccataatgtg aacttgaccc ttagtcggtt
      901 tcaagataca acccatcatt gataaaagtg ttgtctttcc acttcctgaa ggacccataa
      961 gaagaacaag ctcacccgca tctacagata aagttacccc ttttacagcg tgaactctta
     1021 ctgagccctc ttgataaacc ttgtctatcc cgattacttc tatttgtttc gtctttagat
     1081 ccgtgtgttt acgaaatgca gtaagcatgt gggtgagtaa agcgaatccc gttaatatta
     1141 ggtgacgttc ctttattatc tataatagat tgatatgctt tcaatatgtt cacctagatg
     1201 tcggtccaaa tacccaataa aagcctgtag cagttgccac tcagactcct tccttttagt
     1261 attaatttcg gctacaagca aaccaccctt tgctccatcc cagctgatcc aaccactata
     1321 cctttcatgt ttccatcgac cttcacgctc tcggtagaat gtggtacctc ttccccggag
     1381 gtctctctcc ttatccttca agagggtctt aaattttgat ttagttccgg atttagggac
     1441 aatttgcaaa atcttcatac ctaaacctcc atggttgagt attcctcact tctctaagcc
     1501 ctaaatacaa ttactgggtc tacctttgct gctttctgga tcgagatgaa agaagagata
     1561 atgcacataa ttgaggttat gaaaaacata gctattaaaa gtttaggtgt taaaatcaac
     1621 actacaccta ggtattcata caggttcttt gtaagcaagg taaacaaaag accaagggcg
     1681 tatccaattg aggcgcttat gatagcctgt tgaagtataa ttttgtaaat atcaaggttt
     1741 gttgccccga ttgcctttag cgtgccgaac tctcgtatgt gctccatagt tgatgagtat
     1801 attgtttgtc ctacaactac cgttcctaca ataaatccaa gaaacactgt tatgccgacc
     1861 cctaatccca ttcctgtttg aatgctccag tacattcgtg tttttctact gaattgctct
     1921 ttagtataaa catcaactcc gctcattttc tctcttagtt tttcaacaac ttcgtttgca
     1981 ttcactccgt gttcaagttt tacgagaaca aaggttgttt gatcataatc cataattcgt
     2041 gagaattttt ttgcagtttt gtatgaggta aatgctatag gataagtcgt gaatgacctt
     2101 acgccctggc ttattccaac aatctcagtt tgtttatcaa atatttcgac tttatcgcct
     2161 aacttagctc ctaatctctt caaggaggac tcgtcaatta taatggaatt cccaaccttt
     2221 acatctttga tcttgccttc tttcatcttc caggggccac ctacaccgct ttcggtatcg
     2281 aagccgatta tctgtacgga ttcagtgccg ccgctcttaa gtttcatgag cccccatgtc
     2341 tgagcgattt tttcaaccca aagcacgccc tttgtctctc ttactttgtt tatcttattt
     2401 tctgaaaaag gtttagctga gtcaacatta actgtgttct tcgctgtaat ccatatatcc
     2461 gcgggagaat tgtctataag ctgtgatgca ttgcctacaa acgccatata cgcaccaatc
     2521 tgagcggtca taagaacaac cgcaaacatt attcctacaa gggtaatggc aagtctcgtt
     2581 ttatcgtgaa agagattacg cctagcaaca gaaaccatca tgaaaagtat aactgatctg
     2641 tatggagaac ggagctaacg tcgctccagc gtcatccgca taccatattt tgggcggagt
     2701 gtaatggatg gctgaagctc aacacgatga cccggtacaa gactcgtctt ccattttgca
     2761 gcaagcgtgg cgattaccag tatcccttcc atccaagcaa aagattcgcc gatacaacgc
     2821 cttggacccc ccccaaaggg gaaataagcg aatggagggc gagaagcctg ctgctcaggg
     2881 gcccaccgct caggatagaa acgaaaagga tcatggtagt agcgtgggtc a
//
LOCUS       DATDRL010000045         3375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_746861, whole genome shotgun sequence.
ACCESSION   DATDRL010000045 DATDRL010000000
VERSION     DATDRL010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746861"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..2465)
                     /locus_tag="VI935_01635"
     CDS             complement(261..2465)
                     /locus_tag="VI935_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24335.1"
                     /translation="MERGIIKADDRHTARRIKTAMQGNIIRALVELITNADDSYIRIE
                     DEKLPIHKGMIEIFYKKEGYCGLFAVRDYAEGMSIEDVRSSFKKYGAATSGMKTGKRV
                     RGYFGQGAKDALASMVDGKICTFKDDIFVECRLFIENGKPMYEISDPIPATSELRSRH
                     KIDGNGTIAYFNADPQKTGRVPQFDTVQRELANNYLLRKIMTNPQRKVSLLDEDARTA
                     SRQLRYQMPEGKEILVDDFNVSYGHYGNFPIHLYIWRAERELTQTGDDRDGGILIVDD
                     EGIVLGISLFKYDNEPLASRFFGEVRIGRFRELLEKEEPVLSEERDGLLTRHPFSQVL
                     IAEIEKRIETKVKEERLRKQKEDQSKIDREEASRYKKAFSILNEIAEIEAQVIINLAQ
                     NPTDQVEEPPNGFCLYPSSAQITVGKRYVFELRLNTKEVRHGSIIKVTCTSPKIRILA
                     PEIKVSSDDGADIVRKYITVEGSEPNVEGILRATTGNNLSQAKIYVIPEKELLLSEGM
                     VFQPESLTLRPNQPRRVYLLVYIKMIEEGSAIRISPDNDSVHVSKDEIIVNEADATRH
                     VAKYELDIWGEGAGQDAIITAEYETYIALLEVRVRSKEEPGENGRKGMFNEPEFSYDP
                     EPLQRTSYSAETGKVTIYVNFPSVRHYLGDDCRYRKTLPAQVLIADLVAERCFHEIAK
                     KKVEWGVALRPEAVPDRIQRDAYELSRKYGKKLHEALVDQKLIEDAKSIGQS"
     gene            complement(2478..3110)
                     /locus_tag="VI935_01640"
     CDS             complement(2478..3110)
                     /locus_tag="VI935_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24336.1"
                     /translation="MNEKMKKLKPFDSASYDSIDLDRLVIYTTVELGKLGIELSIENI
                     IVGAFKLFPKKFSLFGYPEFPDATRVEKSLWRCKGKKREWIGGKTPHGYLITDRTRTI
                     ATQTEDQLSNPVLGKQKIASRMRRKEGILKEVMSSPAYKKYMSGKGESISEADFCYLL
                     QGTLDSSRETLRENLVSLNKFTEELEREDVLKFLNWLEQRFKNFLNNKRK"
BASE COUNT          864 a          773 c          604 g         1134 t
ORIGIN      
        1 tcaacacctt ttttaacatc atctaattct tcaattgtga gttcacgccc caatttatca
       61 attgcgattc gttgaacatc ttcaacagta ataccaaaaa ttaagtaatt tttattaaga
      121 tatttatcca gcatttttat agacgtttac ctaagtcatt accgatttca cggaccgtag
      181 tataatggtt ttgtaaaatg tttaaaaggt tttctagtaa ttcaaccgtg tccagaattt
      241 caacaactca tgctaactcc ctaactttgg ccaatagatt tagcatcttc gatgagtttt
      301 tggtcaacta acgcttcatg cagttttttg ccatattttc tagatagttc atatgcatcc
      361 cgctgaatcc tgtcaggaac agcctcgggc cttaacgcca caccccactc tactttcttc
      421 tttgcgattt cgtgaaaaca tctttcagca actaaatcag ctattaatac ttgtgctggt
      481 aatgtttttc tataccgaca atcatcaccg aggtagtgcc gaaccgaagg gaagtttaca
      541 tatatagtca ctttgcccgt ctctgcacta taggatgttc gctggagagg ctcaggatcg
      601 tagctgaact caggttcgtt aaacatccct tttcggccat tctcccccgg ctcctctttg
      661 gatcttacgc ggacttctaa aagagctatg tacgtttcgt attcagctgt gatgatggca
      721 tcctgacccg ctccctcgcc ccaaatatcc aattcatatt ttgcaacatg tcttgttgca
      781 tcagcttcgt ttacaataat ttcgtctttc gaaacatgta ctgaatcgtt gtccggtgaa
      841 atcctgattg cgcttccttc ctctatcatt tttatgtaaa caagtagata tacccttcga
      901 ggttgattgg gacgcaatgt taggctttct ggctggaata ccattccctc gctcagcagt
      961 agctcctttt ctggaatcac ataaattttc gcctgggata agttattacc agtagtagct
     1021 cggaggatac cttcaacatt tggctcactt ccttcaactg taatatattt acgtacaatg
     1081 tcagcaccgt catcggatga taccttaatc tcaggggcta gaatgcggat ctttgggcta
     1141 gtgcaggtga ctttaataat tgacccatgg cgaacctctt ttgtattcag gcgaagttca
     1201 aaaacgtaac gcttaccaac ggttatctgt gcagatgaag ggtagagaca gaagccatta
     1261 ggtggctctt ctacttggtc cgttggattt tgtgctagat ttattataac ctgtgcttcg
     1321 atttcagcta tttcattcaa gatgctaaaa gcctttttat atctagatgc ttcttcacga
     1381 tcaatcttac tttggtcctc tttttgtttg cgtaatctct cctcttttac ttttgtctcg
     1441 atacgctttt caatttctgc aataagtacc tgagagaatg ggtggcgagt aagaagacca
     1501 tctcgttctt cgctaagtac gggttcttct ttttcaagga gttctctaaa tcgtcctatt
     1561 ctgacctcac caaaaaagcg cgatgcaagt ggttcattgt cgtatttgaa cagggatatt
     1621 cctagaacaa ttccttcatc atccacaatc agtatccctc catctcggtc atcacctgtc
     1681 tgggtaagtt ctctctccgc acgccagata taaagatgga ttggaaaatt cccgtaatga
     1741 ccataggaaa cattgaaatc atctactaga atttcttttc cttctggcat ttgatatcgt
     1801 agctgccggg aagccgttct agcatcttca tctaagagag atacctttct ctgagggttg
     1861 gtcataatct tgcgtagaag atagttgttc gctaattccc gttggactgt atcgaattga
     1921 ggaaccctgc cagtcttttg aggatcggca ttgaagtaag caatagtccc atttccatca
     1981 atcttatgcc tgctccttag ctctgaggtg gcagggatag ggtcgctgat ttcatacata
     2041 ggcttcccat tttctatgaa aagcctgcac tcaacaaata tatcatcctt gaaggtgcaa
     2101 attttaccat ctaccatgct cgccagggca tctttcgcac cttgaccaaa atagccacga
     2161 actcttttcc ctgttttcat cccactcgtt gccgccccat atttcttaaa actacttcga
     2221 acgtcctcta ttgacatacc ttctgcatag tcacggacgg cgaagagacc acaataacct
     2281 tcctttttgt aaaaaatttc tatcataccc ttatggatgg ggagtttttc atcttctatc
     2341 ctgatgtagc tgtcatcagc atttgttatg agttcaacaa gcgcacgtat aatgttccct
     2401 tgcattgctg ttttgatacg ccgcgctgta tgtctatcat cggccttaat gattcctcgt
     2461 tccatgaaac acctccccta ttttcgtttg ttatttaaga aatttttgaa acgctgttct
     2521 aaccaattga gaaactttaa cacgtcctca cgctcaagtt cttctgtaaa cttattaagt
     2581 gaaacaaggt tttctcttaa agtctctcgt gaagaatcta atgtgccttg caatagatag
     2641 caaaagtcgg cttcggagat agattcaccc tttccgctca tatacttttt ataagcaggt
     2701 gaactcataa cctctttcaa aataccttct ttcctgcgca ttcgtgaagc aatcttttgt
     2761 tttcctaata caggattaga taattggtct tctgtctgtg tcgcaatagt tctggtccta
     2821 tcagtaatta ggtagccgtg cggtgtttta ccccctatcc attctctttt ctttcccttg
     2881 cagcgccata gagatttctc gacacgtgta gcatcaggaa attcagggta cccaaataag
     2941 gagaatttct tcgggaaaag tttgaaagct ccaactatta tgttctcaat ggaaagttca
     3001 atcccgagtt tcccaagttc gactgttgtg taaataacta acctgtcaag gtcaatcgag
     3061 tcataactgg cggaatcaaa tggcttaagt tttttcattt tttcattcat ttatcaccct
     3121 gactatgttt cacatagctc gttcatactc gaagttcgga tatataccta tagatgtttt
     3181 aagtccttat cacctatttt ataaaaacct tgatttttta caatagcttt attgtaaggg
     3241 aagtagaatt tcaaccggta gaaatactcc ccttatacgc ccaatctagt agctcaacgg
     3301 gatgagcggt tttgatgttt aatccatatc gttttatacc catccctatc tgtagcaggc
     3361 agccagggtt tcctg
//
LOCUS       DATDRL010000046         3819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1783241, whole genome shotgun sequence.
ACCESSION   DATDRL010000046 DATDRL010000000
VERSION     DATDRL010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783241"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VI935_01645"
     CDS             complement(<1..330)
                     /locus_tag="VI935_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HLE24337.1"
                     /translation="MNLTNQQISRYSRHLIMPEVGVEGQEKLVSSSVLCIGAGGLGSP
                     LALYLGAAGIGRLGIVDFDVVDFSNLQRQIIHGEAKIGKLKVESAKERIHEINSDIEV
                     ETYNLRLT"
     gene            complement(692..1492)
                     /locus_tag="VI935_01650"
     CDS             complement(692..1492)
                     /locus_tag="VI935_01650"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HLE24338.1"
                     /translation="MTPLFKIRNITYRVATRRDFLKALITGGVGISATPFLGFGEASK
                     FHFTQLIYRGEWDPRPRAFKRLMATLESRTSVETSFERKTLKIESPNLFYYPFLYLSG
                     KESFEPLNAEEKRRLRRFLKLGGTLLVDDVSGEENSAFDSQIRKELLNILPESPFSQI
                     FPDHVMFKSFYLLSLVSGRKIVKPYLEGITFTDEDRTPVIYSRNDLGGAWSEDEFGKW
                     QFECIPGGETQREFAFRLGLNIIMYALTGNYKKDQIHTPFIKRRQRNL"
     gene            1842..2993
                     /locus_tag="VI935_01655"
     CDS             1842..2993
                     /locus_tag="VI935_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLE24339.1"
                     /translation="MNSDKKLFEIYRDEFPVTKSYIYLDHAGVAPVSLSVKRAVEKFL
                     SEATKSGMFNYKQWMDRVEEIRKKCATFIGADPEEIAFVKNTSHGISMVAEGLDWKEG
                     DNLLICEKDFPSNIYPWLNLKRKGIEIGVVPSQNGRILIEDIEGLIDSRTKLLTVSSV
                     NFSSGFKIDLEVVGRLCKEKGILFFVDAIQSLGVIPMNVHEFEIDFLAADGHKWLLSP
                     EGTGIFYCRKELALMVNPPLIGWKSIINESDYDHIDFRLKTNSLRFEEGSLNVMGIFA
                     LGAAIDLLIKVGIDRIQARVIELGDLIIREAEKRDFLVRTPKNKEERGGIISIIGDFD
                     PINVKDRLKEEGILVNVRGGAIRISPHFYNTEYEVLRLFDAIDRVVKSK"
     gene            3074..3334
                     /locus_tag="VI935_01660"
     CDS             3074..3334
                     /locus_tag="VI935_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24340.1"
                     /translation="MDEIKELIEQLSQTLAVLSGNATILKHQYGPVKKAAQDDSLENI
                     KQALINFEMYLGNVKEKIVDAYILWEKIMEEINVNKSKTLNS"
     gene            3614..>3819
                     /locus_tag="VI935_01665"
     CDS             3614..>3819
                     /locus_tag="VI935_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HLE24341.1"
                     /translation="MQVSKTLDYAVRSLTYLSKEPVGRLSIKEISQNQHIPLNYLAKI
                     MRKLVNKGIVQSMVGPEGGYVLRKS"
BASE COUNT         1160 a          675 c          810 g         1174 t
ORIGIN      
        1 agtaagcctt aagttatagg tctcgacttc tatatcagag ttaatttcat gaattctttc
       61 ttttgcagat tctactttaa gttttcctat tttggcttcg ccgtgaatta tctgccgctg
      121 gaggttactg aaatccacta cgtcaaagtc cacgatgcca aggcgtccta tacctgctgc
      181 tccaagataa agtgcaagag gcgatccaag cccacctgct ccaatacaga gaacactcga
      241 tgaaaccaac ttttcctgcc cttccacccc tacttctggc ataatgaggt gtcggctata
      301 acggcttatt tgctgattcg taagattcat tttgtaatac cttccttttg agggttaaag
      361 attaatatat aggttaatac gaaaattcta aattttcaaa ccccccatct tcagattgcg
      421 gaatagaaga aatctttatt gccacagagt tcacggagtt catagagaga tttgttcttt
      481 aattaaaatt ctctattttc tctgcgtgct ctgtggttaa aagcattctt aatttcaaag
      541 attgcagaat agaagaaatc tttattgcca cagagttcac ggagttcata gagaggtttg
      601 ttgtttaatt aaaattctct attttctctg cgtgctctgt ggctataagt tttcttaatt
      661 ccaaattgcc aaatccgaaa tcagcaattt actaaagatt tctctgtctg cgcttgataa
      721 acggggtatg tatctggtct tttttataat ttccagttag tgcgtacata ataatgttta
      781 atcctagtct aaaggcaaac tctctctggg tttctcctcc aggtatacat tcaaactgcc
      841 atttgccaaa ttcatcctcc gaccaagcac cacctaggtc gtttcttgaa taaattactg
      901 gagttctgtc ctcatcggtg aatgtgattc cttcaaggta tggctttaca atcttccttc
      961 cactcactag tgataggaga taaaaggatt taaacatcac gtgatcggga aaaatctgac
     1021 taaatgggga ctcaggaaga atattgagaa gttctttgcg tatttgagaa tcgaatgcag
     1081 agttttcttc ccctgataca tcatctacta gaagagttcc accaagtttt agaaatcttc
     1141 taagccttct tttttcctca gcattaagcg gttcaaaact ttctttacct gatagataaa
     1201 gaaatggata atagaaaagg tttgggcttt cgatcttaag tgttttcctc tcaaacgagg
     1261 tttcaacact tgtccttgac tcaagtgttg ccatgagcct tttgaatgct ctaggacggg
     1321 gatcccactc acctcgatag atgagttggg taaagtgaaa ctttgaggct tctccaaaac
     1381 ccaaaaaggg ggtggcactt attccaaccc ctcctgtaat gagcgcttta agaaagtctc
     1441 tccttgttgc cactctatat gttatattgc ggattttaaa taatggtgtc aagttgatgc
     1501 tttcagtttg tggtgagagc agtttaaaca ggggtagccc acgggatctg tggtagctat
     1561 ccagagggag gttcttttag ggaaccacat gcttgctggt ggacgtaatc ccgtccccat
     1621 tttgaatgcg ggacaggaat gtcccgccta taggtggaat tttaatgtat acggataggc
     1681 ggggttttct aaccccgcct aattgtgtga tgattttcga caagctcagt cctgggacct
     1741 tgttccgacc tgtggtgagc ttgtcttttg ttctcaaaaa cttacctaac cttatcatac
     1801 aggatgattt tattatatat tgttagcaag aatactataa aatgaattca gataagaaac
     1861 tattcgaaat ctacagggat gaattccctg tgactaaaag ttacatttat ctagatcatg
     1921 ctggagtagc tcctgtgtcg ctaagtgtaa agagggcagt tgagaagttc ttatctgaag
     1981 ctacaaagag cggaatgttt aactataaac aatggatgga tagggttgaa gaaattagaa
     2041 agaaatgcgc cacctttatt ggtgctgatc ctgaagagat agcttttgta aaaaatacat
     2101 ctcatggcat ttccatggtg gcagaggggc tagactggaa ggaaggggat aacctactta
     2161 tctgtgagaa ggattttcct tctaatattt acccctggct gaatctaaag agaaagggaa
     2221 tcgaaatagg agttgtgccc tctcaaaatg ggaggattct aattgaggat atcgaggggt
     2281 taattgattc aagaacaaaa cttcttaccg taagctctgt gaatttctca agtggtttca
     2341 aaattgactt ggaggtggtt ggaaggctat gcaaagaaaa ggggattctt ttttttgttg
     2401 atgctattca aagcctgggc gtgattccaa tgaacgtaca tgaatttgaa atagatttcc
     2461 ttgcagcaga cggacataaa tggcttttaa gcccggaagg gactggtata ttctattgta
     2521 gaaaagaact tgccttaatg gttaaccctc ctcttatagg atggaagagt attattaatg
     2581 aatccgatta tgaccacatt gattttcgtc tcaaaactaa ttcactaagg tttgaagagg
     2641 gctcgctcaa tgttatggga atttttgccc ttggcgcagc aattgacctt cttatcaagg
     2701 ttggaattga taggattcag gctagggtca ttgagcttgg ggatcttatt ataagagagg
     2761 ctgaaaaaag ggattttcta gtgagaacac caaaaaataa agaggaaagg ggaggaatta
     2821 tctctattat tggggacttt gacccaatta atgtaaaaga taggttaaaa gaagagggta
     2881 ttctggtaaa tgtaagaggc ggtgcgattc gtatatcacc ccacttctac aatacagaat
     2941 atgaggtatt gagactattc gatgctatag atagagtagt aaaatctaag tagaaaaaac
     3001 atactctgcc ccaagggcac taagcaacaa agaatttttc tttgtgtctc cgtggctaat
     3061 taaggaggag gatatggatg aaattaaaga acttatagaa cagttgagtc aaaccttagc
     3121 tgtgcttagc ggaaacgcaa ccattctcaa acatcaatat gggccggtaa aaaaggcggc
     3181 ccaggatgat tccttggaaa atattaaaca agcactcatt aattttgaga tgtatttggg
     3241 caatgttaaa gagaaaatag ttgatgctta cattctctgg gaaaagatta tggaggagat
     3301 taatgtaaat aagtctaaga cactaaatag ttaaattgta gtaggggctt gattaatcaa
     3361 acccctacat ggggcaaaac aaaggaaatt atggaggggc ggattttaaa ccagccccta
     3421 acgttttctt tacgactgct tgacaatcca tgggtcgcat ctaaaatagc tgttggtatt
     3481 agaacataat ttacatcatc ccttttttag tacctttttc tcaacaaaag ttttatgaag
     3541 acttgacagt tttcgaggat atggtaattt atacatcata gatgcctact atccataata
     3601 gaggtaaagt gatatgcaag taagcaaaac actagactat gcggttaggt cgttaactta
     3661 tttaagtaaa gagcctgtag gaaggttaag cattaaagag atttctcaaa accagcacat
     3721 cccattaaat tacctggcaa agattatgcg aaaacttgtt aataagggga ttgtacaatc
     3781 aatggtagga cctgaaggtg gttatgtact gagaaaatc
//
LOCUS       DATDRL010000047         6079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2569644, whole genome shotgun sequence.
ACCESSION   DATDRL010000047 DATDRL010000000
VERSION     DATDRL010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2569644"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2198
                     /locus_tag="VI935_01670"
     CDS             <1..2198
                     /locus_tag="VI935_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24342.1"
                     /translation="SLQIWEEFPEVRDFRNFKEAKGGKGIPPQGCILEDSTRFLSIPR
                     QQSTQTALPCPSWNYVVSMNCRRGDRSIHDIVRDIATLIPASQRHRLGIIIGVNEKVG
                     IDRPVDYQPSWDDLLGGRGTEALLAEYNIPILLTYYQWTSFREHKDNKTLSPSAVRKS
                     ILQKLAGIGSERQRSITEQKLKEVDNTHQFPFGKAREHLLATQAARDFIGQFDSPNTD
                     VYVHIQDADYVSYNESLKFGDFQGQGTTRLIPADETYLLSKYDRLIEAHKKGHGRLPI
                     VVGGAHVYSPNEDMSPHPYLNTVGCKEWTRFGSEMGNCLKHIIGVQQPYGLYFHEPNT
                     LILSPTSARRLFTHEPLQDWKAIYSKLGAGFKFGIDSEVQDFTRKLFKGSSDSVCRTG
                     MVFSATTVLSTSMKRGADPFTIEFSGTYDPGNTRFFGCARSDLPKLRGMSQEVMHPNK
                     WVSNVSTSFASHRRFDARKVLCALFGIFDPLERFDPQSSESFKTLLINYETHLLENQS
                     SILDSFRSLSSFYDLKKQGKSVAKSLLSLSWECGQVMRLMLLDHICPPRDVNISPAQI
                     GKDVREFLTLRFNFLSTSPVSPTPNTFVETLIHYVPIVKELTPLLLMPVTIKQEEQII
                     QPASSSNVVILHILQHPGAIEEPYTEGEYFCLLGKLKQMVLAGTLTYNQITSWAGYSG
                     VNGARDFLTMERRTFRSKESKWNKLVKESKENKLLDGLLKDKGNPTPTA"
     gene            2290..3567
                     /locus_tag="VI935_01675"
     CDS             2290..3567
                     /locus_tag="VI935_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24343.1"
                     /translation="MKNLKNILFLTLVFCLGEPQHSWAMENEIEALPLEKEKAGKGSP
                     HKRKRSDKYIEEPDLTPPTITSQTSTLKSPAITLRSQPSKTPFARFKEIIEGEPYTPL
                     IPMEKITAAEEDKEGSRKWVKFLSSAEDGETHKWHQTMGKVITALRKENETKRYLPAQ
                     VNFAAVRLVFVRKGKEVIVQDIPYFFASGLPANPNKNGAKRLAADLANKKISDDLKDF
                     NGYGLRFITKAYKGTEEESIGASFTQNLTGIVEDELKKDDPIIGKNRLQTHKKLLEAV
                     DSKSILAMLYFHSEQSIWMFVKEMIEKFKRSYIESYCSSSSSSSSSSPCSPVTHVFVD
                     IASYYDMCWCCGDTFASCCHTNHLGSQVYVRATGCQAYPDPPFITKPTYALRHHRSEF
                     VGYGIGRAFEMPAPDKIDHYKPYIAHALVNDFQ"
     gene            3564..5318
                     /locus_tag="VI935_01680"
     CDS             3564..5318
                     /locus_tag="VI935_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24344.1"
                     /translation="MKKKINFLFFFLYIAGLCFILPGPCFSMIPDETPFEQGRRGVIL
                     YREENYQAASTLLRSALEAGELTFALTYADICHRNLDKGKHRPHEAAMWYVRSAEGGD
                     ADAIAYLWSLSIDLSDKNSEKDKTQQIVHIWARQDADRGFQLYIEEQFTEAAKLLKRA
                     SLNHCLAQELYGDICLRQLDGKPHSYLEASMWYLLSARGGDPQSLAYLKSLVPGILEN
                     DDHESQMKAIVGSWTLCETLPLLKPLDPGKVNNYLSFKYDRKINKTTAQKLPYLFERV
                     ASAIMFNLTGRAHSVIDDQLFPVPESDFPAPIRSCALSARKIAESKFITVPLVAFGYG
                     PVYLSYFKSLDAKLDKKGRFLKKPDKRPIISPIFALLLDALYEVTKGKTANEFTAISH
                     HDYPWLWARIMFWKKQNKEDFSFTSLSEEELLRRLTDMSTPISEEDIKAILEKVESKD
                     ISNDGTDPICKEFDRRMRTFFTTPLSRQEEVVRATEETFYASLRSHQLDCSNQDDLEK
                     LKTGLLMFYCHDLTLRNVSSTVSCHQALQEGQRYFVDLTVTVTFSPNFNSTSITGSNI
                     IKLLENIQKRGVKVSIVQ"
     gene            5567..>6079
                     /locus_tag="VI935_01685"
     CDS             5567..>6079
                     /locus_tag="VI935_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24345.1"
                     /translation="MKKQKLSLIFITCLGLIGIFIVAFNGWSQKERAITREEQLKLPQ
                     SWSVEDASSQPLFTSVEPSPDGKYTLVAQKYPPQDEKQKPLLVITLMENKSHKIIWKT
                     PLDKNFHSPQWSSDGNYISFLSFNEKNIPSLQITPFKTFEPQIALEKEGLAAYLWSPG
                     SHQIALISRDI"
BASE COUNT         1867 a         1195 c         1240 g         1777 t
ORIGIN      
        1 attctcttca aatttgggaa gaatttccgg aagtaaggga ttttagaaat tttaaagaag
       61 ccaagggagg gaagggaatt cctccccaag gctgcatttt ggaagactct acaagattct
      121 tatccattcc aagacagcaa agcactcaaa cagcacttcc ttgtccaagc tggaattacg
      181 ttgtttcaat gaattgtcga cgtggagatc gttccattca tgatattgtt cgagatattg
      241 ctacattaat tccggcctcg cagcgtcata ggctgggcat tatcattgga gttaacgaaa
      301 aagtgggtat agaccgtccc gtagattatc agccttcatg ggatgatctc cttgggggac
      361 ggggcaccga agctcttctt gctgagtata atattcctat tctacttacc tattatcaat
      421 ggacatcttt tcgtgaacat aaagataaca aaaccttgtc tccctctgct gtcaggaagt
      481 ctatcttaca aaaactggct gggattggtt ctgaaaggca gagaagcata acagaacaaa
      541 aacttaaaga agtagataat acccatcagt ttccctttgg caaagctcga gaacatttgt
      601 tggcaacaca agcagcaaga gattttatag gccagtttga ttcccctaac acggacgtct
      661 acgttcacat tcaagatgca gattatgtct cttataacga aagtctaaaa tttggagatt
      721 ttcaaggaca aggaacaacc cggttaattc ccgccgacga gacatatctt ttgagtaagt
      781 acgataggtt gattgaagct cataaaaaag ggcatggccg cctgccaatt gttgtaggcg
      841 gagcgcacgt atatagtcct aatgaagata tgagccctca cccttactta aatacagtag
      901 gttgtaaaga atggactcga tttgggtctg aaatgggaaa ctgcctaaaa catattattg
      961 gggttcaaca gccttatggc ctttatttcc atgagccaaa tactttaatt ttatcaccca
     1021 catccgcgcg tcgtttgttt acacatgaac cgttgcagga ttggaaagcg atatatagca
     1081 agcttggagc aggattcaaa tttggtattg atagcgaagt tcaagatttt acccgaaagc
     1141 tttttaaagg atcctctgat tctgtttgtc gtacaggaat ggttttttct gcaacaactg
     1201 tgctttcaac ttcaatgaag aggggggcag acccgtttac aatagagttc agtggaactt
     1261 atgacccagg caatacccgc tttttcggtt gtgcgaggtc tgatttaccg aaacttcgag
     1321 gaatgagcca agaagttatg caccctaaca aatgggtgtc aaatgtatca acctcatttg
     1381 cctcacatag aagatttgat gcaagaaaag tactctgcgc gctttttggt atttttgacc
     1441 cgttagagag attcgatcca caaagctccg aaagctttaa aactctcctt attaactacg
     1501 agacacatct tctagagaat caaagtagta ttttagattc atttcgttct ttgagttcat
     1561 tttatgatct aaagaaacag ggaaaatcgg ttgcgaagag tctcctctct cttagctggg
     1621 aatgtgggca agtcatgaga ttgatgttgt tagatcatat ttgtccgcca agagatgtaa
     1681 atatcagtcc agctcaaatt ggtaaggacg tgcgggaatt tcttactctt cggtttaatt
     1741 tcctttccac aagtcctgtt tcgcctaccc caaatacatt cgttgaaact cttattcatt
     1801 atgtgccgat agtaaaggaa ttaacgcctt tactcctaat gcccgtaaca attaaacagg
     1861 aagaacagat aatccaaccc gcttcctcat caaatgttgt tatacttcat atactccaac
     1921 atcctggagc tatagaagag ccgtatacag aaggagaata cttttgttta cttggaaagc
     1981 tcaaacagat ggttttggca ggaacgctaa cctacaatca aattacatca tgggcaggct
     2041 acagcggtgt gaatggggcg cgggattttt tgacaatgga gagacgaacg tttaggagca
     2101 aggaaagtaa atggaataaa cttgtgaaag aatcaaagga gaacaaactt cttgatggac
     2161 tcctaaaaga taagggaaac ccgactccca cagcatgagg acaaaaagtt cgatttgatg
     2221 tatgaataat aaaacgagac tatgcagtgc attccggact aaactctaaa agctataaga
     2281 tggtatttta tgaaaaatct taaaaatata ctttttctca cacttgtctt ttgtttaggg
     2341 gaaccccagc actcatgggc aatggagaat gaaatagaag cgctgcctct cgagaaagaa
     2401 aaggcaggaa agggctcacc tcataaaagg aagcgttccg acaaatatat tgaagagcct
     2461 gatttaactc ctcctaccat tacttcccag acctccactt taaaatctcc ggccattact
     2521 ttgcggtctc aaccttcaaa aacccccttt gcccgtttca aagagattat tgaaggtgaa
     2581 ccttacaccc cccttattcc gatggaaaaa atcactgcag ctgaagaaga taaagaaggc
     2641 agcagaaaat gggttaaatt tttgagtagc gctgaggatg gggaaaccca caagtggcat
     2701 caaacaatgg gaaaggttat tacggcctta agaaaggaaa atgaaacaaa acgatacttg
     2761 cctgcgcaag tgaattttgc tgctgtgcgt cttgtctttg ttcgtaaggg aaaggaagtt
     2821 atcgtgcagg atatccctta tttttttgca agtggattgc ctgcaaatcc taataaaaat
     2881 ggggccaaaa ggttagcagc agacctagcg aataaaaaaa ttagtgatga cctgaaagat
     2941 tttaatggtt atgggttaag atttattaca aaagcctata aaggaactga agaagaaagt
     3001 atcggcgcat cgtttacgca aaacttaaca ggaattgtgg aagatgaatt aaagaaagac
     3061 gaccctatta ttggaaaaaa ccgtcttcaa acccataaga aattattaga agctgttgat
     3121 tcaaaaagta ttttagcaat gttatatttt catagtgaac aatcgatttg gatgtttgta
     3181 aaagaaatga tagagaagtt taaaagatca tatattgaat catattgttc ctcttcctct
     3241 tcctcttcct cttcatcacc atgcagtcca gttacacatg tttttgtaga tatagcttct
     3301 tattacgaca tgtgttggtg ttgcggggat acttttgcga gttgttgtca tactaaccat
     3361 ttaggttctc aagtctatgt tagggccact ggctgtcaag cttatcctga cccacctttt
     3421 ataaccaaac ccacatacgc tttgagacat catcgaagcg aatttgtagg gtatgggatc
     3481 ggacgtgctt ttgaaatgcc tgccccagac aaaatcgacc actataaacc ttatattgcc
     3541 cacgcactgg tgaatgactt tcaatgaaaa aaaagataaa ttttcttttc ttctttttat
     3601 atatagcggg tctatgcttt atcttgcccg gtccctgttt ttctatgata cccgatgaaa
     3661 ccccctttga acaaggtcgg aggggggtta ttctttatcg agaagagaat tatcaagctg
     3721 cttccacctt attgcgcagt gctcttgaag caggagaact tacatttgca ctcacttacg
     3781 cagatatttg ccatcgcaac cttgataagg gaaaacatcg tccccatgaa gccgccatgt
     3841 ggtatgtgag gtcggctgag ggaggtgatg cggatgctat agcttatctt tggtctcttt
     3901 ccatagatct ttcggataag aactcggaaa aagataaaac acaacaaatt gttcacatat
     3961 gggcaaggca agatgcggat cgaggttttc aattgtatat agaagaacaa tttactgaag
     4021 ctgctaaatt gctgaagagg gccagtttaa atcactgcct tgctcaggag ttgtatggag
     4081 acatttgctt aaggcaactt gatgggaaac cccactccta ccttgaagcc tccatgtggt
     4141 atcttctttc agctagagga ggagatcctc agtccttagc ttacttaaag agccttgttc
     4201 caggaatatt ggaaaacgat gaccatgagt cacagatgaa agctattgtt gggagttgga
     4261 ctctctgtga aacgctccct cttctaaagc ctttagatcc tggcaaggtt aataactatt
     4321 tgagttttaa atacgatagg aaaattaata agacaacggc acaaaaatta ccttaccttt
     4381 ttgagcgggt tgcaagtgct atcatgttta atttaacagg tcgtgctcat tctgtgattg
     4441 atgatcaact ttttccagtt cctgaatccg acttccccgc ccctattcgt agctgtgctt
     4501 taagtgcaag aaaaattgct gaaagtaaat ttattactgt tcctttagtt gcatttggtt
     4561 atggtcccgt ttacttaagt tactttaaat cccttgatgc taagttagat aaaaaaggga
     4621 ggtttctgaa aaagcccgat aagcgaccta taatttctcc catttttgct ttgcttttgg
     4681 atgctctgta cgaagtaaca aaaggaaaaa cagctaacga gttcactgct atttctcatc
     4741 atgattatcc ttggttatgg gctaggatta tgttttggaa aaaacaaaat aaggaagact
     4801 ttagttttac ctccctttca gaggaggagc ttttacgaag actaacagat atgtctactc
     4861 ctatctctga agaggatatt aaagcaatcc ttgagaaagt cgagtctaag gatatttcca
     4921 atgatggaac agaccccatt tgtaaagaat ttgataggcg aatgcgtacc tttttcacaa
     4981 cacctctttc taggcaggag gaagtcgtaa gggcaacaga agaaactttc tatgcaagct
     5041 taaggtccca ccagttagac tgttcaaacc aagatgattt agagaaactt aaaacaggtc
     5101 ttttaatgtt ttattgtcat gatctaactc tcaggaatgt ttcaagtact gtttcttgtc
     5161 atcaggctct tcaagaggga caacgttatt ttgttgattt aacggtaaca gtgactttct
     5221 ctcctaattt taactcaact tcaataacgg gatcaaatat cattaaacta cttgaaaaca
     5281 ttcaaaaaag aggtgttaag gtatcaatag ttcagtagga tatcatttag tgataagcac
     5341 actcgaacca ttgccgtcgc tatgccgagt ggatcttctg aattttttcg ttgctcttca
     5401 gtatagcaga tatgtgggct aatttgacca gtcctatacc ttatagggca aaacagttag
     5461 cccttggagg gaggtgtaat ttaaaagggt aagtgatttg cctaaccttt tcttaaggat
     5521 ttccaaatat cctatagcta catattcaaa aattaacaaa aaccccatga aaaaacaaaa
     5581 gctgagtctt atttttatta cgtgcctggg attgataggg atttttatcg ttgcctttaa
     5641 tgggtggtct caaaaggaac gtgctataac ccgcgaagag cagctcaagc tcccgcaatc
     5701 atggtcggta gaagacgcca gctctcaacc tctttttaca agcgtggagc cctctccaga
     5761 tggaaagtat acgcttgtag ctcaaaaata tcctccccaa gacgaaaaac aaaagcctct
     5821 tttagttatc actctcatgg agaataagtc ccataaaatt atttggaaaa cgccactcga
     5881 taagaacttt catagtcccc aatggtcttc agatgggaat tacatttcat ttctttcttt
     5941 taatgaaaaa aacattcctt ctttgcagat tacccccttc aaaacatttg agcctcagat
     6001 tgcactcgaa aaggaagggc ttgcagccta tctttggtct ccgggcagcc atcaaatcgc
     6061 gcttatctca agagatata
//
LOCUS       DATDRL010000048         2161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_987882, whole genome shotgun sequence.
ACCESSION   DATDRL010000048 DATDRL010000000
VERSION     DATDRL010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987882"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..579)
                     /locus_tag="VI935_01690"
     CDS             complement(112..579)
                     /locus_tag="VI935_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HLE24346.1"
                     /translation="MPGLFSQAIELEVLKNRFGFSAGGIVLNPSYNIAPRQEVPIVVR
                     EYRSILKMMRWCLVPFWAKEESIGDNMINARAETVTQRLSFKNSFKERRCLVLADGFY
                     DWKDDEKRVLRLLIGLFSKAVSLLLLLVYGTSGKNLEETRFYLLQSLRQKRMI"
     gene            1298..>2161
                     /locus_tag="VI935_01695"
     CDS             1298..>2161
                     /locus_tag="VI935_01695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLE24347.1"
                     /translation="MADYNFDFLFIGGGILGSSGAMALSKRLEERGENARIAVIDVDL
                     EGEHSSTLKNAGGVRATWKNRANVELCKYSIDFYETIREEIQFRELGYFWMHDEVWWQ
                     EIKKNHPLYKEYGLSVELYPKESVPEFLPFVDNLDGIAGLSISRNAGLLDHYSLREYY
                     RREAKKRGVIFFDRLFVKKITIENSRVTEVIANRLGTLDDNIEEKSELIKTCLLNGEI
                     GYTSELASFKCGVLINTAGAWSPRISKLYGFNDEKIKPRRRQMVVIRCAEVDLSSFGL
                     IIDTSHVYFHKD"
BASE COUNT          654 a          394 c          460 g          653 t
ORIGIN      
        1 aagaaactaa aagggggagc aacctattaa catctttcat atcttgatca agccagatat
       61 cttcatcttt ctcactgaga ataacaggca ttctgttatg aattgtatgg attaaatcat
      121 tcgcttctgt cgtaatgatt gtaaaagata gaagcgtgtt tcctccaggt tttttccaga
      181 tgtcccatag accagcaaaa gcaaaaggct cacggctttt gagaacaaac cgataaggag
      241 tcttagaacc cttttctcat cgtctttcca atcatagaaa ccatctgcta aaaccaaaca
      301 tcgtctttct ttaaaagaat tcttgaagct cagtctttgg gttactgtct ctgctctggc
      361 gtttatcata ttgtcgccga tagactcttc ctttgcccag aatggaacaa gacaccatcg
      421 catcatttta agaatgcttc tatattctct gacaactata ggcacttctt gtcttggtgc
      481 gatgttataa cttggattaa gcacaatccc ccctgcagag aatccaaagc ggttctttag
      541 aacctctaac tcaattgctt gactaaagag tcctggcacc caaggtctcc aatctaatgt
      601 aactattttt cactaaattt actcaaactt acacaaaaat gatcatatta aataacaagt
      661 attctttact ttttctttat taaaaatacc tgttgaatac cccgtagttc tactgcgggg
      721 atgaaaacag gttgcccttt gggcaagaaa gaatatagtc ctcaggttta accagaagat
      781 gggtatgagc acggtgaaaa gaactactca tgcagtatct gatcttaatt aacaaagtat
      841 ttagttatag agatcgttac ccgaaccaac cttatatcga tcggactgat gcggggatca
      901 gtccctacat ttttaattgc acaagatgta gcgctcgttc cccgaacggg tagacacacc
      961 atggtgtgtc tctacattag gctgatgagg gtatgagttg ctactttgag aggatagaat
     1021 gcaggctgat acttttaatt tttagatgcc ccgtggctct gccacgggga gtttcacttg
     1081 attacagtgg gtcaggtttg agggtgttta gtaaatgtct attcccaagt tatttccatg
     1141 attttcattt ctactgcatt tacttatcaa tgataaaggt gcaacaacag ggctctatgt
     1201 gataaaatat aaaaagggtt taaaggtagt ttgattaggg gtaggagttt aaagttattt
     1261 ccaccccatt aggtgaggga ttaaccctag ggaaattatg gcagattata attttgattt
     1321 tctttttatt gggggaggta ttttaggttc gtccggagcg atggcgcttt ctaagaggct
     1381 tgaggaaaga ggcgaaaatg cacggatagc agtaattgat gtggatttag agggagaaca
     1441 ttcctccacc cttaaaaatg caggaggggt tcgagctacg tggaaaaatc gtgcaaatgt
     1501 tgaactatgt aaatactcga ttgatttcta cgaaacaatt agagaggaaa tccagtttcg
     1561 tgaactcggt tacttctgga tgcacgatga ggtatggtgg caggaaataa agaagaatca
     1621 tccactttat aaagaatacg ggcttagtgt tgagctttac ccaaaagaat ccgtgccgga
     1681 atttttaccc tttgtagata atctcgatgg aattgctggg ctttcaatct cgagaaatgc
     1741 aggcttgctt gaccattatt cacttagaga atattaccga agagaagcga agaaaagagg
     1801 ggttattttc tttgacaggt tgtttgtaaa gaaaataacc atagaaaaca gtagagtaac
     1861 tgaagttatt gcaaacagac ttggaacact ggatgacaac attgaggaga aatccgagtt
     1921 gatcaaaaca tgtctcttga acggagagat tggttatacc agtgagctcg cttcgtttaa
     1981 atgcggtgta cttataaaca ccgctggggc ttggtctcca agaatttcta agctctacgg
     2041 atttaatgat gaaaagatca aaccacgaag aaggcaaatg gtcgtcatac gttgtgccga
     2101 agttgatctt tcgtcctttg gattaataat tgatacatcg catgtttatt ttcataagga
     2161 c
//
LOCUS       DATDRL010000049         6055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3088715, whole genome shotgun sequence.
ACCESSION   DATDRL010000049 DATDRL010000000
VERSION     DATDRL010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3088715"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..379
                     /locus_tag="VI935_01700"
     tRNA            305..379
                     /locus_tag="VI935_01700"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:340..342,aa:Arg,seq:acg)
     gene            complement(482..1969)
                     /locus_tag="VI935_01705"
     CDS             complement(482..1969)
                     /locus_tag="VI935_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HLE24348.1"
                     /translation="MENLILINVTFNETRVAVTENGTLVELYIERKSQPRTVGNIYKG
                     KVGKVVPGMQAAFIDIGLERSGFISVEDVYEDSFVDLFLEEDESQLSKKRGKFLIQDV
                     LREGQEILIQVLKESTSGKGAKLSSNIALPGKYLVLLGMVDLIGVSRRIENEEERKRL
                     TELIKSSKPKGLGFIARTASTGICEEEIYQDMDNLIRLWEEIKKRAEEQKAPVLVYEE
                     PNLQIRAVRDLITSEVKSIIVDSMDAYRDISDYFLSRFPEFNTNIELYRDNTPLFIQF
                     GIEPEIKKIFEKKAWLKSGGYLIIEEAEALTVIDINTGRYLSGDTQEETIFKINIEAA
                     NEAARQIRLRNLVGIIVIDFIDLKNRSKREELFQTFVDALRKDKARSSIQEISSFGVV
                     QMTRQRVRESLLKNLAESCSYCDGIGYVKSKETASYEIIREIKNQIVNPFARKILVRA
                     SQNVIKTLEELERDNLKHLEAQFSKDILFEPRDDHDFKHFEVKAQ"
     gene            2161..3171
                     /locus_tag="VI935_01710"
     CDS             2161..3171
                     /locus_tag="VI935_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HLE24349.1"
                     /translation="MRLKLILAAVLFGGIALAQGELPEPKGGVTIYVVRPGDSLWNIS
                     KRFFNNPLLWPRLWELNPFIDNPNLIYPGDVLALKSMPPKAPTLPVVKIDPEIGVKKI
                     EEVQPPPPVFYYSRGGDEGFIAPDEWEDMGAILSSEPPKILLGEGDTAYTNVGSEDNV
                     QQGDAFTIFRSSKVVLHPITGKRVGYKVAILGELEIIEVLGNRISSAKITGSYREITR
                     GARIRPLGPFVKEVVIRKGLEKADGYVVESLNNVELTGKGDVIYVDIGEQDGVVPGNT
                     LSIYEHPRYAYDPDAGRNVVIPGALIGKLVVFNVRKDTATGIIVESARQIEKGDVVSV
                     DL"
     gene            complement(3168..3899)
                     /locus_tag="VI935_01715"
     CDS             complement(3168..3899)
                     /locus_tag="VI935_01715"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HLE24350.1"
                     /translation="MEFIVLALSLLSILLLFSNFSIYEDDENVYTQQRFEMVESQLIS
                     RGIQDSKIIQAMLRVPRHKFVPQGLRKLAYSDNPLPIGMDQTISQPYIVALMTELLKL
                     KGGEKVLEVGTGSGYQAAILADIGSEVFTIEILESLSERAQEILRSLGYSNINFRIGD
                     GYRGWPEHAPFDAIIVTAASEHIPEPLIEQLKENGRMVIPIGDMYQELLLITKTDKGI
                     EKKTVTPVRFVPMTGEAQKSKIGEN"
     gene            complement(4141..5151)
                     /gene="meaB"
                     /locus_tag="VI935_01720"
     CDS             complement(4141..5151)
                     /gene="meaB"
                     /locus_tag="VI935_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HLE24351.1"
                     /translation="MTNYITQFIVFLMPITDKEIKLVDKMLGGDRLALARLITMVESR
                     HPSVLEILKGISSKTGRAYTIGVTGPPGAGKSTLVNEVIQAFRKKDNKIGVIAVDPSS
                     PFTGGAVLGDRIRMQNHTLDDNVFIRSLGSRGSHGGLSRATKEIMKLYDAFGMDYIII
                     ETVGVGQTELDIIDLADTVLVVLVPEAGDSVQTMKAGLMEIGDIFIVNKADREGADRL
                     SKEIQFMISLKPKEDEWNIPVVLTSAHESKGIVEVVKEIERHKAFQIDGDLLKEKRVK
                     RREEEFFEILSEIIERKISEDLRSNELRRILDLVKNGELDPYEGAELILSKILHHRQG
                     LN"
     gene            5211..5465
                     /gene="tatA"
                     /locus_tag="VI935_01725"
     CDS             5211..5465
                     /gene="tatA"
                     /locus_tag="VI935_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HLE24352.1"
                     /translation="MFGAFGGFGVWELLIILVILLLIFGPSKLGDLGTALGKGIRGFR
                     KSMKEPDEIDVTPPKEESNRIEGTRLETQSKMAEKKEEVK"
     gene            complement(5489..5911)
                     /gene="mce"
                     /locus_tag="VI935_01730"
     CDS             complement(5489..5911)
                     /gene="mce"
                     /locus_tag="VI935_01730"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HLE24353.1"
                     /translation="MKNGITESLYHVAIVVRNIDEVAKLYEAALGLKVTHVEVVGEQG
                     VKAAMLEPEGGGTAIELLEPLSDNSPISKFLEKRGEGIHHICFLVDNIESALERLKKE
                     GVQLIDQSPRPGAYETRVAFIHPKALKGVLIEIAEKNK"
BASE COUNT         1664 a         1263 c         1169 g         1959 t
ORIGIN      
        1 ttctcaacac tatagggcga cttgagatat tcactatatt tcccccagca agcttcgaaa
       61 ttgtcaaagc cattttctat actaacttca tcgctattct tcacgatctt tacgataaac
      121 cacataatgt cgtaattcct ccctctagaa cttttatttg attaaactca tgtgaaaatt
      181 tttaagaaat tacaccatgc aaaaatacta taacaatatt gttttaaaag tccaacttaa
      241 aattataaaa tggaatggtg taagtattca caactcagga aaactaatgt agatttttaa
      301 ctatgcgccc gtagctcaga ttggatagag cgccggacta cgaatccgaa ggtcgggggt
      361 tcaaatccct ccgggcgcgc ttgcttttcc atgccctttt gttcattact gtacttatgc
      421 catgctgtta ttgctgccta cgaagagaat tcatggatcg aaattttccg gtcagctgtt
      481 ctcattgagc tttaacctca aaatgtttaa aatcatgatc gtcccttggc tcgaaaagta
      541 tgtcctttga gaactgtgct tcgaggtgct taagattatc cctctcaagc tcctctaggg
      601 tttttatgac attctggctt gctcggacca gaatctttct cgcaaaaggg ttcacaattt
      661 ggttttttat ttccctaatg atttcataag aagctgtctc tttggattta acatagccaa
      721 ttccgtcaca gtagctgcag gactctgcta ggtttttaag aagactttct ctcacacgct
      781 ggcgtgtcat ttgaactacg ccaaaggaag atatctcctg tattgatgac cgagctttgt
      841 cttttcttaa agcatcaaca aaagtttgaa atagctcttc cctttttgat cggtttttca
      901 agtcaataaa gtctatgact ataataccaa cgaggtttct aaggcgtatc tgtctggctg
      961 cctcattagc tgcttcaata ttgatcttaa atatagtttc ctcctgagtg tcgccgctta
     1021 ggtatctacc cgtgttgatg tcgattacag tcagagcctc agcttcttca ataatcaaat
     1081 aacccccaga tttaagccat gccttctttt caaaaatctt ctttatctca ggttctatgc
     1141 cgaattgaat aaaaagggga gtattgtctc tgtataattc tatattggtg ttaaactcgg
     1201 gaaagcggga aagaaaataa tctgatatgt ctcgataagc atccattgaa tccactatta
     1261 tgctttttac ttcacttgta atcaggtctc ttacagccct tatttgaagg ttaggctcct
     1321 cgtaaacaag caccggggct ttctgttcct cggccctttt tttaatttct tcccaaagtc
     1381 gaattaaatt atccatatcc tgatatatct cttcttcgca tatccctgtg ctggcagtcc
     1441 tcgcaataaa accaagcccc ttaggcttag aactttttat caattcagta agcctttttc
     1501 tttcttcctc attttctatc cttctcgata cacctataag atcgaccatg ccaagaagaa
     1561 caaggtattt ccctggaagt gcaatattag aagagagttt tgctcccttc ccacttgttg
     1621 attcctttaa aacctgtata agaatctcct gaccctctcg aagcacatct tgaatcagga
     1681 acttacccct ttttttgctt aactgtgatt catcttcctc tagaaataag tcaacaaatg
     1741 aatcctcata cacatcttct accgaaataa agccagacct ctcaaggcct atgtctataa
     1801 aagccgcttg cattccgggg acgaccttcc ccacttttcc tttataaata ttgccaacag
     1861 ttcgaggctg tgattttctc tcgatatata attcgacaag tgttccattc tctgtgacag
     1921 caaccctagt ttcgttaaac gttacattta ttaaaataag gttttccata agttgggctt
     1981 tttctaagca attattgatt ttaattggtt tatcagttaa ttatagacta taagatattt
     2041 agatgaaaga taagaatggt ataaaaacta aaagaaagat agggtgttaa gttttataat
     2101 atatatagta tattattatt cggcctttga gtaatacatt tcttttaagg aggtcaaaga
     2161 atgcgcctaa aattaatttt ggcagcagtt ctttttggag gtatagcctt agcacagggt
     2221 gagctccctg agcctaaagg cggggtgacg atttatgtgg ttcgccctgg tgactctctt
     2281 tggaatatat ctaaaaggtt ttttaataat cctcttcttt ggccccgtct atgggaatta
     2341 aacccattta ttgataaccc caaccttatc tacccaggag atgtactagc tttaaaatct
     2401 atgcctccaa aggcgcctac gcttccagta gtaaaaatag acccagagat tggtgtgaaa
     2461 aagattgaag aggttcagcc tcctccccca gtattttact attcgcgtgg tggagacgaa
     2521 ggtttcatag cccctgatga atgggaagat atgggggcta ttcttagctc agagccacca
     2581 aagattcttc ttggtgaagg agacactgct tatacgaatg tcggctcaga agataatgtc
     2641 caacagggag acgcgtttac aatcttccgt tcttcaaagg tggtgcttca tccaataacc
     2701 ggcaaaaggg ttggatataa ggtggctatt ctcggggagc tcgagatcat agaggtttta
     2761 gggaatagaa tttcatctgc aaagatcaca ggttcataca gggagattac tcggggggca
     2821 cggattagac ccctaggccc ttttgtaaag gaggtagtga ttagaaaggg tttggaaaag
     2881 gctgatgggt acgtagtcga gtctctcaat aatgtggaac taaccggcaa gggagacgta
     2941 atttacgtag atattggtga gcaagacggg gtggttccgg gaaatacatt atcaatctac
     3001 gagcaccccc ggtacgctta tgatccggat gctgggagaa atgtggttat acctggagcc
     3061 cttatcggga agctcgttgt gtttaatgtt cgtaaggata cagctacagg gattattgtc
     3121 gaaagtgcta ggcaaataga gaagggtgat gttgtgagcg tagacctcta attctctccg
     3181 atctttgatt tctgtgcttc tcctgtcatt ggaacgaaac ggaccggtgt gacagttttc
     3241 ttctcaatac ctttgtcggt cttagtaata agaaggagct cttgatacat atctccgatt
     3301 ggtattacca ttcttccatt ttcttttaat tgttcgataa gaggctcggg tatatgttct
     3361 gaagcggctg ttacaataat tgcgtcaaag ggcgcatgct caggccaacc tctatacccg
     3421 tcgccgattc tgaaatttat attactataa ccaagagatc ttaggatctc ctgcgctctt
     3481 tctgataagg attcgagtat ttctatagta aagacctcgc ttccgatatc tgcgagtatt
     3541 gccgcttgat acccggaacc tgtgcctact tctagtacct tttccccacc ctttagtttt
     3601 aaaagctcgg tcataagggc tacgatatag ggttgagata ttgtctgatc cattcctatt
     3661 ggaagtggat tgtcgctata tgccagtttt cttaaacctt gtggaacaaa tttatgcctc
     3721 gggactcgga gcattgcctg tatgattttg gaatcctgaa taccacgtga tatgagttga
     3781 gactctacca tttcgaagcg ctgctgtgta taaacatttt cgtcatcttc gtatatggaa
     3841 aaattggaga ataacaaaag gatagataaa agacttaaag ccaatacaat aaactccatt
     3901 aagatatacc ctagggctaa aagaatgtac ttaaagaaac cctcgggatt ctcccccaat
     3961 tacttctaaa aatccaacat gttggaaaga ttctaccgag gtgaagatta ataatcaagt
     4021 agcagtagat tgaatgatgc gataattcta aacaacagag gctgatttaa ggatttttta
     4081 cttgaatctt ttaatatcta actgaacctt agagaacaag atgtgagttt gtgtttataa
     4141 tcaattcagg ccttgacggt gatgaaggat ttttgaaagt attaactcgg ctccttcata
     4201 cgggtctaat tctccatttt tgactagatc taaaattctt ctaagttcgt tgcttcttaa
     4261 gtcctctgaa atcttacgct cgataatttc cgataaaatc tcaaaaaact cttcttctct
     4321 tctttttact cttttctctt taagtagatc gccatcaatc tggaatgcct tatgcctttc
     4381 aatctctttt accacttcga ctattccctt gctttcatgg gcggaggtaa gaactactgg
     4441 aatattccat tcatcttctt taggtttcag tgaaatcatg aactggattt ctttggatag
     4501 cctatctgcg ccttctctat ctgctttatt cacgatgaaa atgtctccga tctccataag
     4561 cccagctttc atggtttgaa ctgaatcccc tgcttcggga acaagtacga caagcacggt
     4621 atccgcaaga tctattatat cgagttccgt ctgcccaaca cctacggtct caataatgat
     4681 gtaatccatt ccaaaggcgt cgtatagctt cataatttct tttgtagctc ttgaaagccc
     4741 accgtggctt cccctgcttc ctaagctcct tataaatacg ttatcgtcaa gtgtgtgatt
     4801 ttgcattctg attctatctc cgagaactgc tcctcccgtg aatgggcttg atgggtcaac
     4861 tgctattact cctattttat tatctttctt cctaaaagcc tgaataactt catttacgag
     4921 agtgcttttt ccagcacctg gaggacctgt gacacctatt gtgtaagcac gtccggtctt
     4981 tgaacttatc cctttaagga tttctagaac cgatggatga cggctttcaa ccatagttat
     5041 aaggcgtgca agagccaatc tatccccgcc cagcatttta tctacaagtt ttatttcctt
     5101 atcagtgatc ggcataagaa atacgataaa ttgagtgata tagtttgtca aagctcaata
     5161 tgttgacatt aataccctct tcggtaatct tttattgagg aggtaaagat atgtttggcg
     5221 catttggtgg atttggcgtg tgggagcttc tgataattct agtaatcctt cttcttatat
     5281 tcggtccaag taaattggga gaccttggca ctgcacttgg aaagggtatt aggggattta
     5341 gaaaatctat gaaagagcca gatgaaattg atgtcacacc tcctaaagaa gaatccaacc
     5401 gaattgaagg cactcgtctt gaaacgcaga gtaaaatggc agagaaaaaa gaagaggtaa
     5461 aatagtttat ttattcagct tagcataatt acttattttt ttcggcaatc tcgattaaaa
     5521 ctccttttaa agcctttgga tggataaaag caacccttgt ttcataggcg ccagggcgtg
     5581 gtgactgatc tattaattgt acgccttctt ttttgagcct ttcaagcgct gattcaatat
     5641 tgtctacaag aaaacagata tgatgaatgc cttctcctct cttttctaag aactttgaga
     5701 tgggggagtt atcgcttaat ggttcaagaa gttctatagc agttccgcca ccctcgggtt
     5761 cgagcattgc tgctttcact ccctgctctc caacgacttc cacgtgagtg actttaagac
     5821 cgagtgctgc ttcgtagagc tttgccacct cgtcaatgtt tctcacaaca atcgctacgt
     5881 gatagagact ttctgttatt ccatttttca tcgcctacct tcctccgtca gtttcttcta
     5941 aaagcatttt aattggcact ttgagaaaat tcaaatttaa agaacgtgag gtcgacgcaa
     6001 ggacataatt gtagcggaat ccttccttcg ataaaactca ggacaggtta ggatt
//
LOCUS       DATDRL010000050         5209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_241898, whole genome shotgun sequence.
ACCESSION   DATDRL010000050 DATDRL010000000
VERSION     DATDRL010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241898"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="VI935_01735"
     CDS             complement(<1..1527)
                     /locus_tag="VI935_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLE24354.1"
                     /translation="MDKVELDRTVQVLQDNKDRWAKLPVMQKAELVGFLIQGVIAVAD
                     RQVARALEAKGVAPNSIESAEEWLSGPFIVLRNLRLLRQSLEQITRFGVPKIKPEKIH
                     IRSNGQVVVNVFPGSLFDKILYPGFRAEVWMQPEVHCHELHQAMASFYRQSQPQGKVT
                     LILGAGNVSSIGPLDVVYKLFVEGHVCLLKLNPVNDYLGPFIEKAFAPLIQEGFLRLA
                     YGDANVGSYLCEHTGIDEIHITGSAQTHDAIVFGSGEEGADCKRNNMPRLKKRITSEL
                     GNISPVIIVPGKWSEKDLKFQADNIATQLTNNAGFNCNAAKVLITHSNWVQRDAFLNT
                     LRETLRNIPPRRTYYPGAFSRYSRFIEAHPDAEVFGPKSEEIIPWTLIPNVDPDRQSD
                     ICFTQEAFCGIIAETPLQGSDAANFLQNAVEFCNNTLLGTLNACIIVHPQTEAQLRSR
                     LDQAISDLRYGSVGINHWPALSYALGSTTWGAYPGHTLNDIQSGIGVVHNTVMFDKPQ
                     K"
     gene            1708..2901
                     /locus_tag="VI935_01740"
     CDS             1708..2901
                     /locus_tag="VI935_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HLE24355.1"
                     /translation="MGLYSYTRLATYKQCPYRFRLKNIDKISETKTIEQFMGSCVHRV
                     LERLYKNVISAKLIPLEKILEYYHKRWARKWDDSIKFAKKDLSKDDYRALGEKCIEDY
                     YKSYYPFNQSKTIHVEKRVSFALDNEGRYKIVGFIDRLAIAPDGAYEIHDYKTSGNLP
                     TQKDKDEDKQLALYQIGVRDMWRDVNSIKYIWHYLVFNKEISSSRTDRDIEKIRAEKI
                     ALINEIESATEFPPRESSLCNWCSYQSICPLWKHKFIIESIRANELKQEEGFQLVDRL
                     EGLQSERREIETQIEETREALFEYSDREGVDRIFGSAKVAKVKREEKLSFPDSSDEKR
                     IELENVIRKLGKWNEVSLLDVRKLVKKVEEGAWSEDVLEAIGEFGEKEERKSIILVKK
                     TEREE"
     gene            3215..4297
                     /locus_tag="VI935_01745"
     CDS             3215..4297
                     /locus_tag="VI935_01745"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HLE24356.1"
                     /translation="MKNKKYINTRLFKISALVTINILIFLFLFAIIEIGYRIYHDGFS
                     GAINSVPYSNLGTSNWVIYDKDLGYRLNPDREKINNLSIKGEEIAIPKPNGLYRIIFL
                     GDSIPWANPGLVDYTRESLRGKGSFEVINAGVPGYTSYQEVLFFKRYLSQTDPDLVIW
                     TYCLNDNHQFLHRFDENARMLLTEEAKESLQINSYWDKLVSRSYILSLIRFTIISHNK
                     RSNLDNVEYKFIWEKLPDFNIAWKDYSWSGYEENLVELKNLLHKRGGKLAIVIFPHET
                     QVLYGLIKKENYDYIVKPQHKLLSLCKRYDVPCLDLYPSFINEYFQDRRRLFKKDMVH
                     LNKEGHKLATLEISKFLVEKGLLSRN"
     gene            complement(4874..>5209)
                     /locus_tag="VI935_01750"
     CDS             complement(4874..>5209)
                     /locus_tag="VI935_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24357.1"
                     /translation="RRIYIRYLNHLLESHHLALTPEEKLGIKELIRIFNTAKYVWFTL
                     HGLQAKSPIWLHLTRKVFVEIPMARMAELSQEAGVRLIYVFIPPKKNRAKYARTIDEF
                     EEALSREGC"
BASE COUNT         1604 a          921 c         1152 g         1532 t
ORIGIN      
        1 tttttgaggt ttatcgaaca taaccgtatt gtggacaacg ccaatgccgc tttggatgtc
       61 gttcaatgta tgacccgggt atgcgcccca ggtagtagag ccaagggcgt aacttagtgc
      121 cggccagtga ttgataccga cactaccgta gcgaagatct gagatagctt gatctagtcg
      181 gcttcttaat tgagcttcag tttgtgggtg aacaatgatg caagcattaa gcgtgcccaa
      241 gagtgtgtta ttgcagaatt caactgcgtt ctggaggaaa ttggctgcgt cagatccttg
      301 taaaggggtt tcagcaataa ttccacaaaa ggcttcttgt gtaaagcaga tatccgactg
      361 cctatcagga tcgacattag ggataagtgt ccagggaata atctcttcgc tctttggtcc
      421 aaatacctcg gcgtcgggat gtgcttcgat aaagcgtgaa taacggctaa aggcgccggg
      481 gtaataggta cgtcgtggag ggatattgcg tagggtttcg cgtaaggtat ttaggaaggc
      541 gtcacgttga acccagttag aatgtgtgat aagaactttg gcagcgttgc aattgaagcc
      601 agcgttgttg gttagctgag ttgctatatt atcggcttgg aactttaagt ctttctcact
      661 ccatttcccc ggaacaatta tgacagggct aatatttcca agctcactgg tgattcgttt
      721 cttcaagcgt ggcatgttat tgcgtttaca atcggcgcct tcttcccccg aaccaaaaac
      781 gatagcatca tgggtttggg cgctgccagt tatatgaatc tcgtcaatac cagtatgttc
      841 acaaagataa gagccaacat ttgcgtcacc ataggctaat cttagaaagc cttcttgaat
      901 tagtggagca aaggctttct cgatgaatgg accgagatag tcgtttacgg gattaagttt
      961 caagaggcag acatgtcctt ccacaaatag tttgtagact acatcgagag ggccgatgct
     1021 agagacgttg cctgcaccta ggataagagt taccttacct tggggttgag attggcggta
     1081 gaaggaagcc attgcttgat ggagctcatg acagtgtact tcaggctgca tccagacctc
     1141 ggcacgaaac ccaggataga gaatcttatc aaagagagaa ccagggaaga cattaacaac
     1201 cacttggcca tttgatctaa tatggatttt ctcaggtttg atcttaggga caccgaaacg
     1261 ggtaatctgt tctaggcttt gtcttagcaa tcgaagatta cgtaagacga taaaaggacc
     1321 tgaaagccat tcttccgccg attcgataga attaggagcg acccctttag cttcgagcgc
     1381 ccttgcgact tgacgatcgg ctacagctat cactccctgg attaggaatc ctactagttc
     1441 cgctttctgc attaccggaa gcttcgccca acggtcttta ttatcttgta aaacctgaac
     1501 cgtgcgatct aattctactt tatccattat aacctcatct ttataagctc agacctcgtt
     1561 aatatccaaa ctggtttctt atgcatttta gatggcggta tatcccaaac tatttgaaaa
     1621 aaacattgcc gaactaccct tccacaaatt acaattctat tttatattct tacacgatcg
     1681 aaatcttttt tagcgaggca tgtagttttg gggttatatt catacacaag actagctacc
     1741 tataagcagt gtccttatag gtttaggcta aaaaacattg ataagatttc agagacaaaa
     1801 acaatagagc agtttatggg ctcatgtgtt catagggtgc ttgaaaggct ctacaagaat
     1861 gtaatctccg ctaagttaat cccactagaa aagattcttg aatactacca caagagatgg
     1921 gcaaggaaat gggatgatag tataaagttt gcaaaaaagg atttgtctaa agatgactat
     1981 agagctcttg gtgaaaaatg tatcgaggac tactataaga gttattatcc atttaatcaa
     2041 tcaaagacta ttcatgtaga aaagagggtt tcttttgctt tagataatga agggaggtat
     2101 aagattgtcg gtttcataga ccgtcttgca attgctcctg atggtgctta tgagattcac
     2161 gattataaaa caagtggaaa ccttcctaca caaaaagaca aggatgagga taaacaactt
     2221 gcgctatatc agataggcgt aagggatatg tggagggatg taaattcaat taaatacatc
     2281 tggcactact tagttttcaa taaggaaata tcttcgtcta gaactgacag agatatagaa
     2341 aaaataagag ctgagaaaat cgcactcata aacgaaatag agtcagctac tgagtttccc
     2401 ccaagggaat cctcactatg taactggtgt tcgtatcaat caatctgtcc cctatggaag
     2461 cataaattca taatagaaag tattcgagcc aatgaactta aacaggaaga gggatttcaa
     2521 cttgtagata gattagaagg gcttcagagc gaaaggagag agattgagac acaaatagag
     2581 gaaacaaggg aggctttgtt tgaatattca gatagagaag gtgtggacag gattttcgga
     2641 agcgctaagg tcgcaaaagt aaagagagaa gaaaagctta gttttcctga ttcaagtgat
     2701 gaaaaaagga tcgagcttga gaatgtcata agaaaattgg gaaagtggaa cgaggtttca
     2761 ttgcttgatg ttagaaagct tgtcaaaaag gttgaagaag gggcttggag tgaagatgtt
     2821 cttgaggcga taggggagtt tggtgaaaaa gaggagagaa aatctattat actagtgaaa
     2881 aagacggaaa gggaggaata gattggttga cgcttctaaa gtgttttggg gggtcttttc
     2941 tacatttgtg agccatcatg agaattagaa attccaagag ataaagagtt cgagattggg
     3001 actaaagagt atgaaatcaa attcactgac ataggtctta ctaggcttag gactaaaata
     3061 atatgatttt gcaaggaatt tttatatgat ctaacgtgct ttttgtgccc ctttataata
     3121 atagaatata gttgttactt gttgtataca ttgttttttg ttttagcatt aaaacataaa
     3181 tttcaattgc ctgtttaata catagtgtgg gctaatgaaa aataaaaagt acataaacac
     3241 taggcttttc aaaatcagcg cgcttgtcac tatcaacatt ctaatatttc ttttcctttt
     3301 tgcgattatt gaaattggat atagaatata ccatgatggt ttctctggtg ctattaatag
     3361 tgtcccttat tcaaatctag gaacttccaa ctgggtaatt tatgataagg atcttggtta
     3421 tcgtcttaat cctgatagag agaaaattaa caatctctct ataaagggcg aagagatagc
     3481 tattcctaag cctaacggcc tctaccgaat catatttctt ggagactcca ttccgtgggc
     3541 taatccaggc cttgtagatt atacgagaga atctctaaga ggaaaaggaa gtttcgaagt
     3601 gataaacgct ggagtacctg ggtacaccag ttatcaggaa gtgctgtttt ttaaaaggta
     3661 tttatcacaa accgatcctg atttggtgat ttggacttat tgtctgaatg ataatcacca
     3721 atttttacat cgcttcgatg aaaatgctag aatgctcttg acagaagaag ctaaggaaag
     3781 tcttcaaatt aattcctatt gggataaatt agtaagtaga agttatattc tttccttaat
     3841 tcgatttacg attatttctc ataataaaag atccaatctg gataatgtag agtacaagtt
     3901 tatttgggag aagctacctg actttaatat tgcatggaag gactattcat ggtcaggtta
     3961 tgaagaaaat ctagtagagt taaaaaacct ccttcataaa agagggggga aattggcgat
     4021 tgtaatcttt ccacacgaaa ctcaggtttt atacggatta ataaagaagg agaattatga
     4081 ctatatagta aagccgcaac acaaattgct tagtttgtgt aagaggtatg atgttccctg
     4141 tttagacttg tacccaagct tcattaacga gtattttcaa gatagaagaa gattatttaa
     4201 aaaagacatg gtgcatctga ataaagaggg acataaactt gctactctgg aaatctctaa
     4261 gtttttagtg gaaaaaggat tattgtctcg taactagagg aattatatag aatccattat
     4321 atgttcataa ttataagaag gagttagtca gaagtcgttc aacttagttc taaatagtcc
     4381 acaatattgc tgaggttact tttcaggatg ctgtttataa taataaagtt aataaaataa
     4441 taaaagaagg gaataaaagg ggaggcacct ctggaaacaa ccggcgtgcc agcttcaata
     4501 ccaagtgagt atttttctgc cataaccggc tacttatgaa aaaacaatta actatcgccc
     4561 cgtgtcagcc gcgctaccga gcgtagcaag ggtgtgtcag ctgcattatc tatttaggca
     4621 ctccgtttcc tttaggttag acgtttctgt taaataacgg taaatgtgct cggcaataat
     4681 cgcgttccca ttttttgttg gatgcacaga atcgatgaat ttgtctctac gatgaaccgt
     4741 ttgtatactt cgtaatttca gaatccgttt cagttcacga ggccacaccc acgctaatcg
     4801 tgaatcactc gtgcgccctt tttttgtgaa ttcgttcttg tctggggtaa gagctgactg
     4861 tacgtccacg aattcagcac ccttctcgac taagagcttc ttcaaattca tcaatggtgc
     4921 gtgcgtactt cgcgcggttc ttttttggtg gaataaacac ataaatcagc cgcacaccag
     4981 cttcttggct gagctcagcc atccgtgcca tgggtatctc aacaaacacc ttccttgtga
     5041 gatgtagcca aatcggcgat ttcgcctgca atccatgcaa cgtaaaccaa acgtattttg
     5101 cagtgttgaa gatgcgaatg agctccttaa tgcccaattt ttcttcaggg gtgagtgcta
     5161 agtgatggct ttcgagaagg tggttgagat atcgaatata aattcttcg
//
LOCUS       DATDRL010000051         2921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_447343, whole genome shotgun sequence.
ACCESSION   DATDRL010000051 DATDRL010000000
VERSION     DATDRL010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447343"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1430
                     /locus_tag="VI935_01755"
     CDS             201..1430
                     /locus_tag="VI935_01755"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HLE24358.1"
                     /translation="MEIELITTGDEIMSGVTLDTNFHWAAEKLMSLGFALRFHTSVGD
                     DEETIIEALRTAKRRSQAVIISGGLGPTPDDLTAEVASRFFGIPLELNDDALQMIEER
                     FKEQGRKLLEINKRQAYIPKGSQILKNFWGTAPGFQYQKDGIFFFFLPGVPKEFMAMI
                     EEYVIPELDKRTLGRNKFKSRIVKTFGLRESEVAERLRGIEREWVKIGYRPSFPEIHI
                     RISGYGATEEKVERTVSEVIEEIVSRLGEYVFSTEGETLEEVVGKLLSRNRLTLALAE
                     SCTGGLVAHRITNVSGSSNYFERCIVSYSNESKVELLKVPKELIESFGAVSKEVVEEM
                     AQGVRRLANADIGVSISGIAGPTGGTPTKPVGTVFIGISNEKKGTKSQRFQFKDTREE
                     IKLIASEVALDWIRKFF"
     gene            1468..2490
                     /gene="recA"
                     /locus_tag="VI935_01760"
     CDS             1468..2490
                     /gene="recA"
                     /locus_tag="VI935_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HLE24359.1"
                     /translation="MSKEGQTKEVSKEKAIELALSSIEKQFGKGAIMRLGAETQVPEI
                     SVIHTGSIGLDIALGIGGLPRGRIVEIYGPEASGKTTLALHAIAEAQKQGGIVAFVDA
                     EHALDISYAQRLGVRVDDLLISQPDFGEQALEIVDTLVRSGAVDVIVLDSVAALTPKA
                     EIEGEMGDAHVGLQARLMSQALRKITATVSKSKTIVIFVNQTRMKIGVPSYMNPETTT
                     GGTALKFYATVRVDIRRIGSVKDGEEISGNRVRARVVKNKVSPPFREAEFDILFGTGI
                     SQEGELVDIGSNLRIIEKSGAWFSYNGERLGQGRENTRLFLKEHPEIMERIKEEILSK
                     GGIIKK"
     gene            2508..>2921
                     /locus_tag="VI935_01765"
     CDS             2508..>2921
                     /locus_tag="VI935_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HLE24360.1"
                     /translation="MAQMEIDDMLRAAVKADATDVHLKVGSPPVFRIDGSLVFIKEAP
                     SFTQEGLERMAFALMNKWQREKYERTFEMDLAYDIDGLSRFRFNVFQQQGTIAMAIRA
                     IPFEILNFSELNLPPILEKISLEERGLIIATGTTSS"
BASE COUNT          930 a          474 c          753 g          764 t
ORIGIN      
        1 gatttatgca aatattatat tacaggttat tacgaggata gtttgataaa atccggtttc
       61 aagtttagcc acaaataaac acaaatgaga atgagcagga tacacgatgc aggatacatg
      121 atggaagatg aatcatttat cacggatcat ggatcgtgta tcttgcatca ttttttcagc
      181 gcacatcagg agtaattcag atggaaatcg agctcataac tacaggcgat gagataatga
      241 gcggcgtgac gcttgataca aattttcact gggcagcaga gaaactaatg agtctcggtt
      301 ttgctctcag gttccataca agtgtaggcg atgatgaaga aaccataata gaagctctga
      361 gaactgctaa gagaagatca caggcagtaa ttatctctgg cgggcttgga cctacacccg
      421 atgatctaac agccgaggtc gcctctcgat tcttcggaat tccccttgag ctgaatgacg
      481 acgcacttca gatgatagag gagagattta aggaacaggg aagaaagttg ttggaaataa
      541 acaagaggca ggcttacatt cccaagggat cacaaatcct taaaaacttc tggggtactg
      601 ctcccgggtt tcaatatcag aaggatggta tatttttttt cttccttcct ggggtcccga
      661 aagagttcat ggctatgatc gaggagtatg taattccgga actagataag aggactttag
      721 gacggaataa atttaaaagc agaattgtaa aaacatttgg actaagggaa tcagaggttg
      781 cagaaaggct tagagggata gaaagagaat gggtaaaaat cggatataga cctagtttcc
      841 cagagattca tataagaata tctggttatg gtgctacaga agaaaaggtt gagaggacgg
      901 tatcagaagt tatagaagag atagtttcaa ggcttggtga gtacgttttt tctactgaag
      961 gtgagacact agaggaggtt gttggaaaac tcctttcccg aaatagactt acccttgcac
     1021 ttgctgagtc atgcaccggc gggcttgttg cacataggat cacaaacgtt tcaggaagct
     1081 ctaattattt tgaaagatgt atagtctctt acagcaacga atccaaggta gagttgctga
     1141 aggtaccaaa agaattaatt gaatctttcg gggcagttag taaagaggta gtagaggaga
     1201 tggctcaagg ggttagaaga ttagcaaatg cagatatagg tgtttcaata tccggtattg
     1261 caggaccaac cgggggtacg cctacgaaac ctgtagggac tgttttcatc gggatttcta
     1321 atgagaaaaa agggacaaaa tctcagcgat ttcagtttaa ggacacgagg gaggaaataa
     1381 aattaatcgc ttcagaggtt gcacttgatt ggattaggaa atttttttaa ataatgtata
     1441 atttatcaat tcaggaaaca tatcgttatg tctaaagagg gacagactaa agaggtaagc
     1501 aaggaaaagg cgatagagct tgccttatct tctatcgaga agcaatttgg aaagggcgct
     1561 ataatgcgcc ttggggctga aactcaagta cctgagatct ccgtaataca cacaggttcc
     1621 attggtttgg atatagcgct tggtattgga ggacttccaa ggggtaggat tgtggaaatc
     1681 tacggacctg aggcttctgg aaaaactaca ctcgcccttc acgctatagc cgaggcacaa
     1741 aaacaaggtg gtatagttgc atttgttgat gcggaacatg cacttgatat aagctatgcc
     1801 cagaggcttg gagtcagggt ggatgatcta ctaatttcac agccagattt tggtgaacag
     1861 gcacttgaaa tcgttgacac ccttgtaaga agtggcgctg ttgacgtgat agttctcgat
     1921 tctgttgcag cattaacacc aaaggcagag attgagggcg aaatgggaga cgctcatgtc
     1981 ggacttcagg caaggcttat gtcacaggct ttaagaaaga tcacggctac agtgagcaaa
     2041 tcaaaaacaa tcgtaatttt tgtaaaccaa acacgtatga agattggagt cccttcttat
     2101 atgaatcctg aaacaacaac tggcgggacg gcactaaagt tttatgccac tgtaagagta
     2161 gatataagaa gaattggctc tgtaaaagat ggagaggaga tttcaggaaa cagggtaaga
     2221 gcaagggttg taaaaaataa ggtctctcct ccttttaggg aagcagagtt cgatattctt
     2281 tttggaacgg ggatttctca agagggtgag ttagtggata ttggttcaaa cttaaggata
     2341 atagaaaaga gcggggcttg gttttcatat aatggggaaa ggctaggtca gggaagagaa
     2401 aatactagat tatttttaaa ggaacaccct gagattatgg agagaattaa agaagagatt
     2461 ctctcaaagg gtgggattat taagaaataa aatccgaggt gaagtagatg gcacagatgg
     2521 agatcgacga tatgttgaga gccgcagtta aagcagatgc tactgatgta catcttaagg
     2581 tcggaagtcc tccggtcttt cgtattgatg ggtcgcttgt atttataaag gaggcgccta
     2641 gctttacgca ggaaggtttg gagaggatgg ccttcgcttt gatgaataag tggcaaagag
     2701 agaagtatga acgaaccttt gagatggacc ttgcatacga tatagatgga ctatcacggt
     2761 tcaggtttaa tgtctttcaa caacagggaa caatagctat ggctataagg gcgattcctt
     2821 tcgagatcct gaatttttct gagttaaatc taccgcctat acttgagaag atttctctgg
     2881 aggagcgcgg gttaataatt gcaactggaa cgacgagtag c
//
LOCUS       DATDRL010000052        13011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3406806, whole genome shotgun sequence.
ACCESSION   DATDRL010000052 DATDRL010000000
VERSION     DATDRL010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13011
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3406806"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="VI935_01770"
     CDS             <1..1300
                     /locus_tag="VI935_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24361.1"
                     /translation="ECMVIKLHGDYLDTRIKNSPKELEKYSQPLNKLLDRIFHEYGLV
                     VCGWSAEWDTALNKALQRCKSHRFQTFWMIRGEQGDKTKKLIQLRQAEEIIIKDADTF
                     FKELEEKVLSLQEFDRPHPLSVKTAVTTLKKYLRDDNQDIRLQEMITEEVERLYQKLS
                     PENFPVNNYPTSEELLKRIQLYNSYTETLLAIMINGCYWGKESHEHLWIKCLQRIATP
                     HGEREVYEIWNKLRLYPALLLLYGGGIISIVAGKYPTFSGLLKKPKLRLGSQEYPALF
                     YLIPERVIEQSTLSQIGMQRNPLFLNENIFKVLREPIKTFLSSGIDYQKFFDRFEYLH
                     ALIFADFWKKQTQGNIGRSTIWGPVGYFAFREYYGQEPENLIPAEISKEIKQEQLDAG
                     DDWPPLKAGLFDGSAERFQFIKNEFDTWVREAYVRYHVHF"
     gene            1585..2892
                     /locus_tag="VI935_01775"
     CDS             1585..2892
                     /locus_tag="VI935_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24362.1"
                     /translation="MSKYPKGLDVAVDYRFLDAVFQRRSRRFGLGMEIERGPLKYKSK
                     HKSVPLTELEEAILVWTGLGIKSINLSDFPPHVGLDLEMQFTSKTIPALGDVHRTELF
                     YTNDEGTYMIKMHDKKPDDFRGLEGLNREERVDRILELFRESKIKMEDKRANLPDRPP
                     GIASHNLWNVNKPGTTVFMPVTDLSACIINLYYFYMRPDHRFNFVDELHGMRPPGTAG
                     WIKKGFIDEGKRMPLIEAELRFANGFIAEQAFMGQNMALALQALGLGGWLFSGFASMF
                     MLGGTPFFKGLGFRFISPKLKGETGNPNPVAVGKDGLFQAFCPPYYKDMGEAVEALND
                     LKWSNWATHKMPYRNPEGIMQEVERPSKEEIQIVKDICNYVYDTYGRFPAFSDPMFLR
                     FMVQAHHADLDFYDEFYPEGAYTENHKNHFKLWHPEIPDPFEK"
     gene            2991..3956
                     /locus_tag="VI935_01780"
     CDS             2991..3956
                     /locus_tag="VI935_01780"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HLE24363.1"
                     /translation="MKKVFITANLPGKSVELLKRDFHVEVFPEERPASRNELLKGAKD
                     AHAIITMLFDKVDRTLIDSCQNLLIISNCAVGTENIDIHYATKKRIFVTNTPGVLTET
                     TADLAWALILSVSRRLVEGDIFLRDGKFIGWRPLLLLGVNVYGKTLGIYGMGRIGTAV
                     ARRAKGFDMQVIYNNRRRKDEVEREVRANHVDYPTLLRESDFLVITAPLNEESQGRFG
                     IEEFRKMKRTSVLVNVGRGQIIKEKELAIALKEGIIWGAGLDVYEREPIVEEGLLNLK
                     NAVLIPHLGSATEETRTRMADMAVENVVLALSGNIPKNLVNPKAL"
     gene            complement(4042..4530)
                     /locus_tag="VI935_01785"
     CDS             complement(4042..4530)
                     /locus_tag="VI935_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24364.1"
                     /translation="MDDFDDSDYDSPSNYKGFDLSDIDEKELYDVMEKIDLIHKELKT
                     INELCKKLISIYEPSKQSIENLKTIEPDVHQTIMEEAIKLYGDEKGKEFVELILGKLD
                     EDSLKLQISKAKAFIISYERQFDEEDIEPILPSEFQNSDVFKTMRNLTGGKNPLPDLS
                     SR"
     gene            4854..5141
                     /locus_tag="VI935_01790"
     CDS             4854..5141
                     /locus_tag="VI935_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HLE24365.1"
                     /translation="MGKERVTAIIHGRVHGVFYRASAREKAIELGLTGWVRNRLDKTV
                     EVVAEGDKKQLDNLIEWCRVGPPDAYVTEVEVKWEPYNGEFKGFSVKYREH"
     gene            5153..5797
                     /locus_tag="VI935_01795"
     CDS             5153..5797
                     /locus_tag="VI935_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLE24366.1"
                     /translation="MLIKCIPGGSFLTNCYIIADEETKEGILIDPGSQVEEVLEEVER
                     LGLNVKKIVNTHAHIDHAAGVEDAKKTLRAEFYLHPKEEPVLQALPESAMRFPEFKKV
                     KVPKVEKYIEDGDEVEFGKYKAKVIHTPGHTYGGICLLIDDHVFTGDTLFAGSVGRVD
                     LTGGTTMRELVRSIKTKLMTLPPSYTVYPGHGPYTTIGIEKESNPFIAGGVILF"
     gene            5799..6068
                     /locus_tag="VI935_01800"
     CDS             5799..6068
                     /locus_tag="VI935_01800"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HLE24367.1"
                     /translation="MKKSIVKTTLIIILISCVIYLFTREGLHVHELYQENERIKGKIE
                     ELKMSNKELEKRISTLKNDKLYIEKVAREELGMIKQGEKVYKFEE"
     gene            6181..6645
                     /locus_tag="VI935_01805"
     CDS             6181..6645
                     /locus_tag="VI935_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24368.1"
                     /translation="MVGLFIAILIFQKLHHFLKYTNELSLLERLEAAFLFAIFFEVLS
                     ESPGRTLFPLIYIITPIFFAYLGWQEAVLAVVLVAIIETTNHSNGIEVILQLLILLIS
                     TFGLGYLIKRNENLKYGRKQLINSIRIVTNKIFKPARTEEKTRFNFTFSYNG"
     gene            6683..8182
                     /locus_tag="VI935_01810"
     CDS             6683..8182
                     /locus_tag="VI935_01810"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HLE24369.1"
                     /translation="MSELLSPHSVVLYIKGKDGFFEIEDFISSSEKYIDRSQKLHIRG
                     GYLGWVVKTSTPILVGEIKNHKENLIYYTKNIPVKSILAVPLILKDGKNESVNEQDTI
                     GILLVDSLKKDAFEEREKQIVMLISNKIVDTLIKFRMSEKIKLSLSGLTSFYEYTNKL
                     SSTLELDAIIDHVVVVLGKAIGADIVGLTLKDKETNTSILKRTGIDRRLEIEEKTFPN
                     LDTLVGLVSESQKYFYSEDLSAREKYRSVFGKEIDFAWGINKIKSIFIYSLKETKSPF
                     HQENENSIGSIIIGRKTEKAFSEEERNLIEIMCQEAANSISNSLTYNRAKELAIRDSL
                     TGLYNHRYFQERLDHEIARSDRFPEMLSLILMDVDNLKLLNDKYGHQAGDLILISIAQ
                     LISSSLRDIDIVARYGGDEFAILLLHTNEKGSKVIGEKLNHKVEKTALKFKGKELSVT
                     VSLGIATYPENASSKAALIEKADSALYEAKRLGKNRTLHYENIAEEAVK"
     gene            8461..9288
                     /gene="rsmA"
                     /locus_tag="VI935_01815"
     CDS             8461..9288
                     /gene="rsmA"
                     /locus_tag="VI935_01815"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HLE24370.1"
                     /translation="MRRPISSPKEFFQAEGIRPRKGLGQSFIHDKNILERVLEIAELS
                     KEDEVLEIGAGLGALTLFLGERSRRVIAIEKDNRLIEKLRQNVSHLENVEIISGDALQ
                     IDIRELYRGNKIKVVSNLPYSISSPILFKLLEERDIFSLLVLMLQSEVGERINALPGG
                     KQYGSISVLIQAYMDVSVELRVPPEAFWPKPKVWSVVVKLVPLSSPRTYIPEEKLFKK
                     IVKAAFSSRRKMLGNSLSVLFPKEKVRDILEKSGIDGKRRAETLTIEEFGKLAVSSL"
     gene            complement(9300..10736)
                     /gene="dacB"
                     /locus_tag="VI935_01820"
     CDS             complement(9300..10736)
                     /gene="dacB"
                     /locus_tag="VI935_01820"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HLE24371.1"
                     /translation="MKKLYVSISAIALLFFSQIVFSKNSSEYPHRLLESIPREALSNG
                     RLGVVVKSLTTDEFIFQYNSDKLFVPASNVKVLTSVSALSILNPDYRFKTEFYSGGDI
                     TNGVIHGGLYIKGYGDPTLSTEHLESIAQEFKSLGIKEIRGGIIVDESYFDNVRYGKG
                     WKEKWKGDVFSPPISALSLNYNTFYIKVYPSKPGRIPIVALEPKGTNINVITKAITTN
                     KGGRLSAVWLEGGKTIKLSGGISRKTPSYTLEVTTRNPALYTGSAFKRILEDAGIRVA
                     GYIIMGDVPDWAGVFYTHFSDPLYLIIAEFNKNSVNIIGENIIKTLGAEVIAEPGTWE
                     KGAQVVSSFLDKIGIKDRFEIVDGSGLSPLNRVSPNTMTEVLEYAYNNQIISLKFLSS
                     LSIGGVDGTLKRRFKRSDVEGRVMAKTGHLNNVNALSGYMFTKSGDVLAFSILANGLG
                     PRATSFQNSLLEELVDCCVSNNARDSSL"
     gene            complement(10831..>13011)
                     /gene="polA"
                     /locus_tag="VI935_01825"
     CDS             complement(10831..>13011)
                     /gene="polA"
                     /locus_tag="VI935_01825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HLE24372.1"
                     /translation="LSGDATDNIPGVRGVGEKTASELIGKFRTIESLYENLNELPQRQ
                     RDLLEANKESAILSKRLVTIETSIPMSVSLEGFEYKGFNKEKLREIFEELEFKSLLRE
                     LGDNEKVQEEIAIIESSGGKVSYDDYHLVLTERDLDMVLERIDLTKEVSIDLETTSQS
                     PMRAELVGVALSPAPHESYYIPVAHRSMVFDSSKQLNPKYVLEKLKPIVEDNLIKKIG
                     QNLKYEFIVLERNGLRLRGISCDTMVAAHLIDASRLSYSLDELAKYYLEHKTITYKDV
                     TGVGRDKINFEEVELERAKVYACEDADVAMILSKKLASELEKLTLLDVFKDIELKFIE
                     VLARMEMNGVKVNSELLERLSIDFEKSLEKIAEEVYTQVGFRFNLNSPIQLREILFDK
                     LGFPQKKMTKSGEASTDVEVLTELSKFHPVPEKVLEHRGLSKLKSTYIDSFPKLINPR
                     TGRIHTSFNQAGTSTGRLSSSDPNLQNIPVKTLEGRRIREAFIPEDGFLLLSADYSQI
                     ELRLLAHFSEDESLVSAFLAGSDIHNRTAAEIFGVSEDMVTPEMRRRSKTINFGIIYG
                     ISPFGLSKQLGISNTIAKSYINEYFKRYRKVKSYTEASIREAERRGYAVTILGRRRPI
                     PELKSKDRTTRGIGERAAINTPIQGSAADIIKIAMIKIHEKLSRDYRSRMILQVHDEL
                     LFEIHESEIDELSRIIKKEMEKAWQLRVPIRVDIGIGKNWAEAH"
BASE COUNT         4163 a         2516 c         2561 g         3771 t
ORIGIN      
        1 agagtgcatg gtaatcaaat tacatggtga ttacctagac acacgaataa agaactcgcc
       61 taaagaactg gaaaagtata gtcagccatt aaataaatta ctggacagaa tatttcacga
      121 gtacggactt gtagtatgtg gatggtcagc cgaatgggat accgccctaa ataaagctct
      181 gcaaagatgc aagagtcata gattccaaac cttctggatg ataagaggtg aacaaggaga
      241 caaaacaaaa aagttgatac aacttagaca ggctgaagaa atcataatca aagatgccga
      301 tacctttttt aaggaattag aagaaaaggt tctatcttta caagaatttg acagacctca
      361 cccgctttct gtaaaaactg ccgtcaccac tttaaaaaaa tacctcaggg atgacaacca
      421 agacattcgt cttcaagaga tgattacaga ggaagttgaa agattatacc aaaaactatc
      481 acctgagaat tttccagtca acaattatcc tacctcagaa gaacttctaa aaagaataca
      541 gctttataat tcttacacag agacactatt agcaattatg attaatggat gttactgggg
      601 taaggaatcc catgaacacc tttggataaa atgcctccaa aggatagcca ctccccacgg
      661 cgaaagagaa gtatatgaaa tttggaataa gcttagactt tatcctgcgc ttcttttgct
      721 ctatggcggt ggaatcattt ctatcgttgc cggaaaatat cctacttttt ctggattact
      781 taaaaaacct aagcttaggt tggggagcca ggaatatccc gccctatttt acttaattcc
      841 agaaagagtt atagaacagt cgacactatc tcaaataggt atgcaaagaa atcccttatt
      901 tttgaatgaa aatattttca aagtactacg agaacctatt aaaacctttt tatcttctgg
      961 tattgactat cagaaatttt ttgaccggtt tgaatatctt cacgctctta tcttcgctga
     1021 tttttggaag aaacagactc aaggaaatat aggaagatca actatatggg gaccagtcgg
     1081 atattttgcc ttcagagaat attatgggca agaacctgaa aaccttatac ctgcagaaat
     1141 atccaaagaa ataaaacaag agcaattaga tgcaggtgat gactggccgc ctttgaaggc
     1201 tggtcttttt gacggttctg ctgaaagatt tcaatttata aaaaatgaat ttgacacttg
     1261 ggtcagggag gcgtatgtac gctaccatgt acatttttaa accatccttg agaacgtata
     1321 ttgaaaactc ttttccctcc ctcctatttt atcttctaat aaagacactc attttaaaat
     1381 tattggataa aataaacaca tttgctaaac taagttacgt ttgatttatt tactggtgat
     1441 agataacata gctgggtagg caacgagtct tgctcgttgc cttttaaccc accagcaaga
     1501 ctggtgggct accctctatc tattaccaga ttaaatcttc aaagaccgaa agcttagaaa
     1561 ccaaaaagag aagagggaaa atatatgagc aaatatccaa aagggcttga tgttgcagtt
     1621 gattataggt ttcttgatgc ggtttttcag aggaggtcaa gaagattcgg gctggggatg
     1681 gaaattgaaa gaggaccgct taaatataag tcaaagcata aatctgttcc ccttacagaa
     1741 cttgaagaag caattctagt ttggacagga cttggaatca aaagtataaa tttatctgac
     1801 tttccacctc acgtgggact tgaccttgag atgcagttta caagtaagac catccctgca
     1861 cttggcgatg tccacagaac tgagcttttc tacactaacg acgaaggcac atacatgata
     1921 aaaatgcatg ataaaaagcc cgatgatttt agagggcttg agggactaaa tcgagaagaa
     1981 agggttgata ggattcttga actattcagg gaatcaaaaa taaagatgga ggataaacga
     2041 gctaatcttc cagaccgccc accgggaatt gcttcacata acctatggaa tgtgaataaa
     2101 ccaggcacaa ccgtttttat gccggtcaca gacctttcag cctgcattat aaacctctac
     2161 tatttctata tgagaccaga ccacaggttt aatttcgtag atgagcttca cggaatgaga
     2221 ccccctggaa ctgcgggatg gattaaaaag ggatttatag atgaaggcaa gcgaatgccc
     2281 ctcattgaag ctgagctcag gtttgcaaat ggatttatag cagagcaggc atttatgggt
     2341 caaaacatgg cgcttgcgct tcaagcactt gggcttgggg gatggctatt cagcgggttt
     2401 gctagcatgt ttatgcttgg gggtacgccg ttttttaaag gacttgggtt cagatttata
     2461 agcccgaagc tcaaaggtga aactggaaat ccaaatcccg ttgcagttgg caaagacggc
     2521 ctatttcagg cattttgtcc cccttattat aaggatatgg gtgaggccgt agaggcactt
     2581 aatgacctta agtggagcaa ctgggcgaca cacaaaatgc catacagaaa ccctgaagga
     2641 ataatgcaag aagttgagag acctagtaaa gaggagatcc agattgtaaa ggatatttgc
     2701 aactatgttt atgacaccta tggcagattc cctgccttct cagacccgat gtttctaagg
     2761 tttatggttc aggctcatca tgcagacctg gatttttatg atgagtttta tccggagggt
     2821 gcatacacag aaaatcacaa aaaccacttt aaactctggc accctgaaat acccgacccc
     2881 tttgaaaaat aaaaaagtgg ccacagagaa cacagagcag aaaaatgatt ttcaaatttt
     2941 tgtttttctt cactttaacc tctgtgtcct cagtggctaa agaataaaat atgaaaaagg
     3001 tatttataac tgcaaatctt ccaggaaaat ctgttgaact tctaaaaagg gatttccatg
     3061 tagaggtgtt tcctgaagag cgtccagcat cacggaacga attactgaag ggagcaaagg
     3121 acgctcatgc tataattaca atgcttttcg ataaggtaga tagaacactt attgattcat
     3181 gtcaaaatct cctgatcatc tccaattgcg ctgttggcac cgaaaatatt gatatccatt
     3241 atgctacaaa aaaaagaatt tttgttacaa acactcccgg cgtccttaca gaaacgactg
     3301 ccgatttggc ttgggctttg attctctccg tttctcgaag gttagtggaa ggggatattt
     3361 ttctgaggga tgggaaattt ataggatgga gaccgttact tcttctggga gtaaatgttt
     3421 atgggaaaac tctaggcatc tacggaatgg ggcgaattgg aactgctgtt gccaggcggg
     3481 caaaaggttt tgatatgcag gttatatata ataatagaag aagaaaagat gaagtggaaa
     3541 gagaagttag agcaaatcat gtagattatc caactctact tagggaatcc gactttcttg
     3601 taataacggc gcctctaaat gaggagagcc aagggcgatt tgggattgaa gaatttagga
     3661 agatgaaaag aacatctgta cttgtaaatg ttggtcgtgg gcagatcata aaggaaaaag
     3721 agcttgccat cgcactcaaa gaagggatta tatggggggc tggtcttgat gtttatgaaa
     3781 gagaaccgat tgtagaagag ggacttctaa atttaaaaaa cgctgttctt attccccatc
     3841 ttggaagtgc tactgaggaa actagaacaa gaatggcaga tatggcggtt gaaaacgtag
     3901 ttttagcgct cagcgggaat atacctaaaa atttggttaa tccgaaggcc ctttaagtta
     3961 gtgactcgtg gctcgtgatt caatacagga gattcacgat acacgtggcc cgattcacga
     4021 accacgcacc aaaaaaagac tttatctgga cgagagatca ggaagcgggt tcttaccgcc
     4081 agtcagattc cgcatcgttt taaaaacatc gctattttga aactcagagg gaagaattgg
     4141 ctcaatgtct tcttcatcga attgtctttc atatgagatt ataaaagcct ttgccttaga
     4201 tatttgaagt tttaaagaat cttcatccag tttacctaat atgagctcaa caaattcctt
     4261 acccttttca tccccatata gcttaatcgc ttcttccatg attgtctgat ggacatcggg
     4321 ctcgattgtt tttaagtttt caatactctg cttgcttggt tcataaatag aaattagttt
     4381 tttacacagc tcattaatgg tttttagctc cttatggatt aaatctattt tctccatcac
     4441 gtcgtacaac tctttctcat caatatcaga taggtcaaaa cccttataat tactagggct
     4501 gtcataatcg gaatcgtcga aatcatccat gatcatcaga gctccccccc tttaatgaca
     4561 tttcagcaca ttttttaatg taattcaata ggcaattcac attgaacaaa aatgagcgaa
     4621 aatgaatttg ggagtttatc ataatttatt ttaagcatac ttagcaactt ttcaaggcgt
     4681 gatggtttgt aaaacctgta ctagctaaat taacaacatc ttacttccaa ctacactcta
     4741 atttcagcct ttttcaagga attaaaaatc aagaaaagta attgttccca attgttccca
     4801 attgttccca atattgacag tatgttacgg ttttggtaac atgagcctgt ccaatgggca
     4861 aggaaagggt aaccgccatt atccatggca gagtgcatgg agtcttttat agagcatcgg
     4921 cgagggagaa agcaatagag ctcgggctaa caggctgggt aagaaacagg cttgataaaa
     4981 cagtcgaagt cgttgcagag ggtgataaaa agcagcttga caatttaatc gagtggtgtc
     5041 gtgtaggacc ccccgatgct tatgtaaccg aagtagaggt aaaatgggaa ccatacaacg
     5101 gggaattcaa aggtttttca gttaaataca gggaacatta gttgaggcaa caatgctaat
     5161 taaatgcata ccaggtggat ctttccttac taattgttat ataattgctg acgaagaaac
     5221 aaaggaagga atcttgatag accccggaag ccaagtcgaa gaagtacttg aagaagtaga
     5281 aagattgggc ttaaatgtta aaaagatcgt aaatactcat gcccatattg atcatgcagc
     5341 aggtgtggag gatgcgaaga aaaccctaag ggcagaattt tatcttcatc caaaagaaga
     5401 acctgtgctt caagcccttc ctgaatctgc gatgcgattc ccagaattca agaaggtcaa
     5461 agtccctaaa gttgaaaagt acatagaaga tggtgacgaa gtcgagtttg gcaaatataa
     5521 ggcaaaggta atacacacac caggacatac ctatggtgga atctgcctac ttattgatga
     5581 ccatgttttt accggagata ccctttttgc aggtagcgtt ggaagagtcg atttgactgg
     5641 tggcacaact atgagagagc tagtccgctc tatcaaaact aagcttatga cattaccccc
     5701 ttcatatact gtatacccag gacacggccc gtatactact atcggaattg agaaggaatc
     5761 aaatcctttc attgcaggtg gagtaatact tttttaatat gaaaaaatca atcgttaaaa
     5821 ctacgttaat aataatatta atatcatgtg tgatatatct ctttacaaga gaaggccttc
     5881 atgtgcatga actatatcaa gaaaacgagc gaataaaagg taaaattgaa gagcttaaaa
     5941 tgtcaaataa agagcttgag aagagaataa gtacccttaa aaacgataaa ctatatatag
     6001 aaaaggttgc aagagaagaa ctcggtatga ttaaacaagg tgaaaaagtc tataaatttg
     6061 aagaataagc taagcaactt cttatacctt cttaacagaa cctattcact tttcctctct
     6121 attttagtct tatgcctaat ttataatggt agctttcaaa gcttcaagcg gtattcattc
     6181 ttggtaggtt tatttatagc tattttgatt tttcaaaagt tgcatcactt tttaaaatat
     6241 accaacgaac tcagcctatt agaaaggtta gaggctgcat ttcttttcgc catctttttt
     6301 gaagtattat ctgaatcacc aggaagaact ctttttccac ttatatatat tattacccca
     6361 attttctttg cataccttgg atggcaagaa gctgtactag cagttgtact cgttgccatc
     6421 atagaaacta caaaccattc caatggtatt gaagttatcc ttcaactatt aatacttttg
     6481 atctcaacat ttggtctagg ttatttgatt aaacgaaatg aaaatttaaa gtatggtaga
     6541 aagcaactca taaatagtat aagaattgta acaaataaga tttttaagcc tgctagaaca
     6601 gaagaaaaaa ccagatttaa ctttaccttc tcctataatg gataaaaaac ccaaaagtga
     6661 tataagaagt tcaattgaat tcttgagcga gcttttgtct cctcatagtg ttgtacttta
     6721 tataaaaggt aaggatggct ttttcgagat tgaagatttc atatcaagca gtgaaaaata
     6781 tatagataga agccagaagc tacatattcg tggtggttat cttggatggg tggtaaaaac
     6841 aagcacaccc attctagttg gtgagattaa gaaccacaaa gaaaacctga tctactatac
     6901 aaaaaatatt cctgtaaaat caattcttgc agttcctctt attctcaaag atggaaaaaa
     6961 tgaatcggtt aatgagcagg atacaatcgg catactttta gttgacagcc tcaagaaaga
     7021 cgcctttgaa gaaagggaaa agcaaatcgt tatgctcata tcaaacaaga tagttgatac
     7081 gctgatcaaa ttccgaatgt ctgaaaagat taagctgagt ttgagtggcc taacctcttt
     7141 ttatgagtat acaaataaac ttagttccac cctagagctt gatgctatca tagatcatgt
     7201 ggttgtagta ttaggtaagg ctataggagc agatatagta ggattaacat taaaagataa
     7261 agaaacaaac acaagcatcc taaagcggac aggaatagac agacgattag aaatagaaga
     7321 gaaaacattc ccaaacctag ataccctagt tggattagtc agcgaaagcc aaaaatattt
     7381 ctactccgaa gacctatcag caagagaaaa gtacagaagc gtgtttggta aggaaataga
     7441 ttttgcttgg ggaataaata aaataaagtc aatctttatc tattccctta aagaaactaa
     7501 gagtcccttt catcaagaaa atgaaaactc aatcggatcc attataatcg gaagaaaaac
     7561 agagaaggcg tttagtgaag aagagaggaa tcttatagag atcatgtgtc aagaagcggc
     7621 taattctatt tcaaattctc ttacctataa tagggccaag gagcttgcga taagggatag
     7681 tttaacaggg ctttacaacc ataggtattt tcaagaaagg ctagatcatg aaattgcacg
     7741 ttctgataga ttccctgaaa tgctatcgct tattttaatg gatgtagata acttaaagct
     7801 ccttaatgat aaatatggtc accaagcagg agatttgatt ctcatatcta ttgctcagtt
     7861 aatttccagt tcacttagag atatagatat agtagcaagg tatggtggtg acgagtttgc
     7921 aatactactt ttacacacaa acgaaaaggg atcaaaggtt ataggagaaa aattgaatca
     7981 taaggtcgaa aagactgccc tgaaatttaa gggaaaagag cttagtgtta ctgtaagcct
     8041 aggaattgca acatatccag agaatgcatc atcaaaggct gctttgattg aaaaggcaga
     8101 ttccgcacta tatgaggcaa aacgccttgg aaaaaataga actctccatt atgagaatat
     8161 cgctgaggaa gctgtgaagt aatttacggt tatagagaac aggctggtta tgctttaaag
     8221 gttgtacttt ccctggctag cgtaagtaaa taataattaa aaacgaattt gcgatttaaa
     8281 attttttctt ctttatcaat aattttacta aaataactat caccccactg tacactgcta
     8341 actttgctgg aattaaggca taaataagca ttaacacaaa agaatgactt tctccgtata
     8401 cactgacttt cagagacaat tatgcttaac tataatttga gatcaaagcg caaaaactat
     8461 atgagacgac caatatcatc acctaaagaa ttcttccaag cggagggtat aaggcctaga
     8521 aaaggtcttg ggcagagttt tatacatgac aagaatattc ttgagcgcgt attggagata
     8581 gccgaacttt caaaagaaga cgaagtgctg gagattgggg ctggacttgg tgccttaaca
     8641 ctttttctag gagaaagaag cagaagagta atagctattg aaaaggataa tagactcata
     8701 gaaaagctta gacaaaatgt ttctcatcta gaaaacgtag aaataatttc cggcgatgct
     8761 ctacaaatag acattagaga gttgtacagg ggcaataaaa ttaaggttgt ctcaaatctt
     8821 ccctacagta tttcatcccc tatcctattt aagcttctag aagaaaggga tattttttca
     8881 cttttagttc tcatgcttca gagcgaggta ggagagagga taaatgcttt accagggggt
     8941 aaacagtatg gttcgatatc agttcttatt caggcataca tggatgtaag cgttgaactc
     9001 cgtgtgccac cggaggcctt ctggcccaag cccaaggtgt ggtcagtggt cgtaaagtta
     9061 gtaccactat cgagtccacg aacttatatc ccagaggaaa agctcttcaa aaaaatcgtt
     9121 aaagcagctt tttcatcaag acggaaaatg cttgggaatt ccctttctgt cctttttcca
     9181 aaagaaaaag taagagacat cctagaaaaa tcaggcatag atggaaaaag gcgagctgaa
     9241 accctaacaa tagaagaatt tggaaaacta gccgtctcaa gcctttaaaa ggtataagcc
     9301 tagaggcttg aatctctagc attattgctt acacaacaat ctacaagctc ttcaagtaag
     9361 ctattttgga aggaagtcgc ccttggtcca agcccatttg ccaagattga gaaagcgagt
     9421 acatcaccac tcttagtgaa catataccct gaaagcgcgt ttacattatt tagatgacca
     9481 gttttagcca ttaccctacc ctcaacgtct gacctcttga atcttcgttt aagtgttccg
     9541 tcaacgccac cgattgaaag agaactcaga aactttaaag aaataatttg gttattatag
     9601 gcgtattcta aaacttcggt cattgtattt ggagaaaccc tgtttaacgg ggagagcccc
     9661 gatccgtcaa ctatctcgaa tctatcttta attccaattt tatctagaaa gcttgaaact
     9721 acttgtgctc ctttctccca agttcctggc tcggcaatga cctcagctcc gagcgtttta
     9781 ataatatttt cgccgataat gttaacgctg tttttattga actctgcaat tattaaatat
     9841 agagggtcag aaaagtgggt gtagaaaaca cctgcccaat cgggtacatc tcccataatt
     9901 atatatcctg ccacccttat gcctgcatct tcaagaatcc tcttaaaggc gctgcctgta
     9961 tacagcgcag ggtttctcgt agtaacctct agcgtgtaag aaggggtttt gcgagatatt
    10021 ccaccactga gctttatcgt ctttccaccc tcaagccaaa ccgcagaaag cctcccccct
    10081 ttattggttg taatagcctt agttataaca ttgatattcg tacctttcgg ttccaatgcc
    10141 actatcggta ttcttccagg ttttgaagga taaactttta tatagaaggt attgtagtta
    10201 agagagagcg cgctgatagg agggctaaag acatctccct tccatttttc cttccatcct
    10261 tttccgtatc taacattatc aaaataagat tcgtctacta ttattccgcc cctaatttct
    10321 tttatcccta aactcttaaa ctcctgggct atggattcga gatgctctgt gctgagagtt
    10381 ggatcaccat atcccttaat gtaaagaccc ccatgaatca caccatttgt gatgtcccct
    10441 cctgagtaga actcagtttt aaatctatag tcagggttaa gaattgaaag cgcactaaca
    10501 gaagttaaaa cctttacgtt tgatgcaggg acaaaaagct tgtctgaatt atactgaaat
    10561 ataaactcgt ctgttgttag ggacttcaca acaaccccga gccttccgtt actcaacgcc
    10621 tctctgggga ttgattctaa aagcctatga ggatactcgg aactattttt tgaaaaaacg
    10681 atctgggaga aaaaaagcag agctatcgct gaaattgata catagagctt tttcatcctg
    10741 gtgaaaggat gctaacatac atcttaaaac atatcaatcg tgaatcgtgt atcgagaatc
    10801 gaaccccgaa tcacgaacca cggtccacac ttaatgtgcc tctgcccagt ttttacctat
    10861 cccaatatct acccttatcg gcacccgaag ttgccaggcc ttttccatct ctttttttat
    10921 gatcctagaa agctcatcaa tctcactttc atggatttca aataacagtt cgtcatgaac
    10981 ctgaagaatc atccgagacc tataatctct tgaaagcttc tcatggattt taatcatggc
    11041 tatcttaatt atatccgcag cagagccttg gatcggggtg ttaattgcgg cacgctctcc
    11101 aattcctcgt gtagtcctat ccttagactt aagctctgga atcgggcggc ggcgcccgag
    11161 aatggttact gcgtatcccc gccgttcggc ttcccgaatg gacgcttccg tataggattt
    11221 taccttccta tagcgcttaa aatattcatt gatatagctt ttagcaatcg tgtttgatat
    11281 accaagctgc tttgaaagac cgaatgggct aatcccataa atgatcccaa aattaatggt
    11341 tttagaacgc cttctcatct caggtgttac catgtcttct gatactccaa agatttcggc
    11401 tgcagttcgg ttatgaatat cactcccagc caaaaacgca ctcacaaggc tttcatcctc
    11461 ggagaaatgc gctaggagcc ttagttctat ctgggaatag tcggctgaga gaagtaaaaa
    11521 cccatcttca ggtataaaag cctcccttat ccttctccct tcaagggttt ttaccggaat
    11581 gttctgtaga ttgggatcac ttgagcttaa tctccctgta gaggttcccg cctgattaaa
    11641 agatgtgtga atccttcctg tccggggatt aataagcttt ggaaaggagt caatgtaggt
    11701 tgatttaagc tttgagagcc ctctatgctc aagcaccttt tcaggtacag ggtggaactt
    11761 ggaaagctct gttaatacct caacatctgt ggacgcctcg ccagacttag tcattttctt
    11821 ttgtggaaat cctagcttgt caaagagaat ctccctaagc tgaataggag aattcaaatt
    11881 aaacctaaat ccaacttggg tgtaaacctc ttctgcgatt ttctcaaggc tcttttcaaa
    11941 atcaattgat agcctctcga gaagctctga attgacttta accccgttca tctccattcg
    12001 ggcaagtacc tctatgaatt taagttcgat gtctttaaaa acatcgagga gagttaattt
    12061 ctccagctca cttgcgagtt tcttactgag tatcatagcc acatcagcgt cctcgcaggc
    12121 atagaccttc gccctttcta attctacttc ttcaaagttt attttatccc tgccaacacc
    12181 agttacgtct ttgtatgtaa tggttttgtg ttcaagataa tacttagcaa gttcgtcaag
    12241 gctgtaactt aaccttgatg catcaataag atgtgctgct accatcgtat cgcatgaaat
    12301 tccccggagc ctaagtccat ttcgctcaag tactataaat tcatatttta gattctgtcc
    12361 tatttttttt atcaaattgt cttcgactat aggttttaat ttttcaagaa catacttagg
    12421 attaagttgt tttgaggaat caaataccat tgacctatgg gcaacaggaa tataataaga
    12481 ttcgtgggga gccggcgaaa gagcaacgcc aacgagctct gccctcattg ggctctgtga
    12541 cgtagtttca aggtctatcg aaacctcctt agtcaaatct attctttcga gcaccatgtc
    12601 caaatccctt tcggttaaaa ccagatgata atcatcgtaa gaaactttgc caccagagga
    12661 ttcaattata gcaatctctt cttgaacctt ttcgttatca ccaagctcac gaagaagtga
    12721 tttaaactca agctcttcaa aaatctctct tagcttttcc ttgttaaacc ctttatattc
    12781 aaacccctct aggcttacgc tcattggaat agaggtctct atcgtaacta gccttttgct
    12841 tagaatagca ctttccttgt ttgcctctaa aagatccctc tgtctttgtg ggagttcatt
    12901 cagattctca tagaggcttt ctatagtcct aaatttgcct attagctctg aggcggtttt
    12961 ctctcctacc ccacgaaccc ctgggatgtt atccgtcgca tctccagaaa g
//
LOCUS       DATDRL010000053        16954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3342301, whole genome shotgun sequence.
ACCESSION   DATDRL010000053 DATDRL010000000
VERSION     DATDRL010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 16954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16954
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3342301"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VI935_01830"
     CDS             <1..351
                     /locus_tag="VI935_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24373.1"
                     /translation="RPNRIAGHLPTSHFLKVWDEGKDNFKNDPPNANVTILGKTEGAT
                     NVVVELSNPRFKGEDLIYDVQVLDGTPPATGDATAVFIDWWVSPRGMVCHRNWYTGQA
                     WCHWPGPYYYPRYY"
     gene            532..693
                     /locus_tag="VI935_01835"
                     /pseudo
     CDS             532..693
                     /locus_tag="VI935_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774638.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
     gene            complement(875..2584)
                     /locus_tag="VI935_01840"
     CDS             complement(875..2584)
                     /locus_tag="VI935_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLE24374.1"
                     /translation="MAVKTKRPKDLKYAVNIERRRPFDVRWYEREDRTKWVLPLILRE
                     RAEKLSDKPFLQFGDNPPFSFSKINLLANRIANGIMHLGIKKGEKVAVYMPNSSDYVL
                     TWFGILKMGGIMVPINTAYKMDFLQYILDSSDSKVLFVAEEYLNRMPPIADRIPQLER
                     VIVWTRTGAAEFDSQGFDSKPMVPFSEFINDRGEDEPDVKITHLDHARLMYTSGTTGR
                     SKGVVRPCQADYQSARNYAEVMDVWGDDVCFTCLPLFHSNAMVMSVYPALIMGARAII
                     QEKYSATQFWKWMKDYGVTKFNMVGTMSYFMWNTPPVPEENEHGVKLVLGSPAPHDII
                     EPFMERFNIKFMEGYGLTEIGQVTFMRPGEPFRVGSCGKESPGYEIKIVDPETDEELP
                     RGSVGEIVVRPRIPNIMLHYYHKMPEKTVQDFRNFWFHTGDAGRMDKKGYIYFVDRVK
                     DYIRRRGENISSFEVERIVNSHPAVAESAAVGVKAQTGGYAEDEVMIFVVKNPGMDVS
                     PEELLKFLEPKMPYFMIPRFIRFVSSFPKTGTERVQKNKLRDEGITPDTWDRESAGYQ
                     IKR"
     gene            complement(2797..3975)
                     /locus_tag="VI935_01845"
     CDS             complement(2797..3975)
                     /locus_tag="VI935_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24375.1"
                     /translation="MQGLNLISSRYVGDFHEGEIYHHGAVQITPMRNREYINKYRDGL
                     ILGANPELSLHLGYEDPDYKLKKEKYGIEKSALVSQGFIFNAGFGLSVHDISFNAIAN
                     LSYSDLRFGVPVYEGDVIYAKSEVLGKEFRKDGATNGSVQVKTTIYNQRGESVLEYVR
                     QVLVRAEGGKTYPKASTIKSQPDKIDIKKTVLPKGFRDLSKELLGKNGKTFEDFRVED
                     VYHGLFEDAIDLVTFSWLQIATLNDAAVHHNPSSNFIGYGGGVKGRAEGEVSGHFPLA
                     YHLGMNSGSHDAPTYPGDIVQVLFAGGKEGPHEKIRARIEITGAIHELPLLNGRGDIG
                     IITIKLTGEKYVTDGGMKALEAARFGGIDQIYEEGGNRFLKVLTLEQVLAVPTRRAFK
                     "
     gene            4095..4339
                     /locus_tag="VI935_01850"
                     /pseudo
     CDS             4095..4339
                     /locus_tag="VI935_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4693..6036
                     /locus_tag="VI935_01855"
     CDS             4693..6036
                     /locus_tag="VI935_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HLE24376.1"
                     /translation="MVKLKKGTDTTILGLTPVITLLTLVLLTSLSFAQEAAPPTVDTG
                     DTAWMLTSSALVLMMTIPGLFLFYGGLVRSKNTLGTVMQSFIIVGLISIQWALWGYSL
                     AFGPDKGGIIGSLAWFGLKGVGLDPNPDYAATIPHQAFMIYQLMFAIITPALITGAFA
                     ERAKFSTFIIFILLWATFIYDPLAHWVWGVGGWLRKLGALDFAGGTVVHISSGISALA
                     AAIIFGRRIGYGHEAMPPHNLPFSIIGASLLWVGWFGFNAGSALAAGGLATSAFVVTH
                     MATAAAALSWMFMDWIFRGKPTVLGAASGAVAGLVAITPASGFVGPISSIIIGIGAGV
                     FCSIACNVKPKLGYDDSLDVVGVHGIGGTWGALATGLFASKAINAAGNDGLFFGNPGQ
                     LWIQFVAVAVTWIFAFIGTLIILSILKVIMGLRVSEEEEQMGLDLSQHEEKSYTL"
     gene            complement(6146..7276)
                     /locus_tag="VI935_01860"
     CDS             complement(6146..7276)
                     /locus_tag="VI935_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HLE24377.1"
                     /translation="MTTESIVAEGLKGVVIGKSEICFIDGKRGKLIYRGYDIHDLVKH
                     SNFEEVVYLLWYGKLPTKKNFDDLYKSLVINRALSKEAISLLKSFPKKATPMEVLRTA
                     ISTLSLTDRSADDNSFESNVKKAVNLTAKTATIIAAFDRIRNGKEPIEPNPNLNHAAN
                     FLYMLAGKVPDEMVSRFFDICLILHADHELNASTFAARVTSSTLSDLYSAITSAVGTL
                     KGPLHGGANIEVMKMLTDMGTVEIAKEYVRTRLAERKRIMGFGHRVYKTEDPRATHLR
                     RMSKEMGEKTGETKWYEMSRVIEDFMLNEKGMYPNVDFYSASTYYSMGIPIDLYTPIF
                     ALSRVSGWIAHVLEQYSNNTLIRPVAEYVGPNDLTYLPMDKR"
     gene            complement(7615..8004)
                     /locus_tag="VI935_01865"
     CDS             complement(7615..8004)
                     /locus_tag="VI935_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLE24378.1"
                     /translation="MSLRKITKKKVVTVRPDDTVVKAAKLMEEKNVGSVVVVENRKPV
                     GILTDRDIAIRVVAKKADINSILVKDVMTKKIVTGRDNQRVAELAKVMHENGIRRVPI
                     VDKKGMLSGIITLDDLLYMMGLKARFF"
     gene            complement(8410..9468)
                     /locus_tag="VI935_01870"
     CDS             complement(8410..9468)
                     /locus_tag="VI935_01870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLE24379.1"
                     /translation="MGKDIKFGLSAPMPGADLNGVLNFSVKADKLGFDSVWYPDHVVF
                     VSPTDAHEAWTIATVAATKTKNIRLGTVSDPHRMHPAVFAQRLATIDHLSEGRVCLTL
                     GVGESMNLDAYGIKWDRPLARLREAMKVMQMLWKSEGPVDFHGNFYTLSQAFLQVKPY
                     KRDKIPMYIATHTPKGLRLTGELGDGWLPIDLTPGLYAEYLNNVKQGAQSVGRTLDDN
                     FDPGLWVFTSLGKNVDEAYKTLEPFKYVLIMQEQLKRAGYDIEIPEDYKGLNYFNVIP
                     QDAAGRQKFREIGKFFPREAVLDFTITGSKRDCIKKIEKYIDKGVRHFVLFYRFSPDP
                     EKALKTYAKQIIPYFKNR"
     gene            10003..11028
                     /locus_tag="VI935_01875"
     CDS             10003..11028
                     /locus_tag="VI935_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLE24380.1"
                     /translation="MKFGIGLFSLQTHPENPSSHAEICRDTLTQVKLAEEVNFDSAWI
                     SEHHFLDDGYCPSPAVIASAMAAVTSRIKIGSAGIILPLHNPVRVAEDSALVDNISNG
                     RFNVGVVLGYRKDEFDGFGIPMKQRPSRMEEGIEVIVKSWTEEKFSYEGKRFKLSNIN
                     VTPKPVQKPHPPIYIGAFEEPAIRRAGRLGCPLLIGPGRTMQMVRDTLGFYNDAAQKA
                     GRNPNNVEHILLREAYIAEDKKKAKGEADKYIISMYKYYFGLGVKMFVRGKQLTGLDD
                     PMFDHLAEDRFIIGDPEDCIREINRYRNELGINYITCRMVFPQATHETISKCIELFGK
                     EIIPILS"
     regulatory      11302..11494
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            11574..13601
                     /locus_tag="VI935_01880"
     CDS             11574..13601
                     /locus_tag="VI935_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLE24381.1"
                     /translation="MKKLLFILTLFYFASNLLYLFKPSLILFDKRHVQNKAAGKEVFI
                     DRDFLFYNYAFAQESGKTLEPIIVEETPLKPPLDYPSAFSTVIDLEDFGGEYNTTSEI
                     LSFSPGVFVRDFGGFGQLQTISIRGSSNDQAVVLLDGVRLNTALGGIDLSTIPLDYVE
                     KFEIIRGGSSALVGTDAIGGVVNIVTKKTDKPFTFGSVTYGSFNTLSINLSRAQKFKN
                     LSYLFSYTHAQSEGDFKFESVNDIILTRINNEFLSESVLTKFEYDLNGWKVGFLNEFY
                     FDDKGVPGLGEFQQPDSNQKDVRNLTSVKLSKEKFLRDDFDFEIIFFNRFDRLKFTNP
                     DPTIGVPIDTLQRDFAFGIDPKITWFAPFNQIVTFATEIKEEILTDKDFGEPKRFTFS
                     AFLSDEIHLFDDLFIIAPIVRYDLIITSAEEQVNTSPGVIGSPRAITTTESEFSPKLG
                     IIVSPIKHLSIKGNVGRSFRVPSFTELFFPEEGFIGGNPDLKPESSIDFDVGLVFSHP
                     RIGFEINYFRNQIDDLILFVFVSATRIEPLNVGEVTQQGIETSVVLRPINFIELFAGY
                     TFLDGKLEDTGAQLPGRPKNKFDLRGVFKYLFLKLFWETHIVGKIPLSPFPNSATTDA
                     STVSNVGGTVEWKQVFATLEAKNVFNNLDVQDALDFPLPGRTIFFTPGVKF"
     gene            13831..15342
                     /locus_tag="VI935_01885"
     CDS             13831..15342
                     /locus_tag="VI935_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24382.1"
                     /translation="MRSSSNLLIFGILGIVLWGCGGGGSGGETQQFFALTNSPEIVVG
                     GIKGSVGGGAIVQVRNLSNGQERQTTSSGDGSFVITLEGSIGDTIGISAPGVDTAVEV
                     MSVSDVSSKIKQEIMSGAVRRNLAAIEGFPTAIEIRGDRAYVLNFFNRIEIFNLDQDP
                     PQRIGEIVLPPESDPVSIAFLDDTFAYVVNNIGQSIALVNVETMQCELLIVRSDSVPP
                     ATSICQNQVITVDPEFFEDPSAIAIAPNGKVYVTNNNLDDFFEPIGNGFITVISSETN
                     QVINRIDATRANSDGITIIDNRLYVVNTGDTDFDLVTEENSCDFDFPPSIDVIDTQSD
                     TRITINISLSTQNPNVCSPNRIEPTPDGKFGYMGLGLVGALLKVDLENNSIINGPSDP
                     IIITPLDEFNFTADIKIRNDGLGFITLFNTDQIAVFDTATDEINPFPFIAFFPAGFGA
                     ENPDSEFFEGVQSLAIRPGIPGIDFQGADIFYITSVGQSTHLGSINTGPLTPE"
     gene            15491..16435
                     /locus_tag="VI935_01890"
     CDS             15491..16435
                     /locus_tag="VI935_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HLE24383.1"
                     /translation="MRICSFLPSATEIVYALGLGENLVGATHECDYPPEAESKPRVIA
                     SLINPEDLTSYEIDKAVSENYKAGKSTYIVDIETLRRTNPDIILTQGLCDVCAVSGNE
                     VIESVKVLGHNPKIISLEPNTLGEILNTILLVGEATERKEQSEYLVNNLRSRIAAIKS
                     LLGEEKDRPRVYCMEWLDPSYIAGHWVPEMVRIAGGDYGLGKEGKPSFKVSWDEIFDF
                     APQVIVLMPCGFNIERTLREIEILTSNEYWHRLPAVRKGHVYIVDANSYFSRPGPRIV
                     DGLEILAKIIHPELCTFEIPADSVLNLRNYMHLVAFLG"
     gene            16611..>16954
                     /locus_tag="VI935_01895"
     CDS             16611..>16954
                     /locus_tag="VI935_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HLE24384.1"
                     /translation="MRNFIKGFLPIFIPLLILLTLLKVSYSENFPRRIVSLSPSITEI
                     IYGLGAWENVVGVTIYSDFPPEATNLPKVGGWVNPNLEAILALKPDLVIMIEDQDRIF
                     GDKIRKIGLKTLA"
BASE COUNT         4940 a         3566 c         3361 g         5087 t
ORIGIN      
        1 cgtccaaaca gaatagctgg tcatttgcct acctcgcatt tcctaaaggt ttgggatgaa
       61 ggtaaggata actttaaaaa cgaccctcca aacgctaatg taacgattct tggtaaaaca
      121 gaaggagcga ccaacgttgt agtggaatta agcaatccac gttttaaagg tgaagatctt
      181 atatacgatg ttcaggtttt agatggaacc ccacctgcaa cgggcgacgc cactgcagta
      241 tttattgatt ggtgggtcag ccctcgtggt atggtctgtc accgcaactg gtacactggt
      301 caagcatggt gtcattggcc aggaccctac tattaccccc gatactatta atcaggatta
      361 atctgggtaa gtcacgagca agactcgtga ctggtagtcc gactattaaa aagaatgacc
      421 agtgtagggg cagggcttgc acctgcaccc ctcaaggtgt acagaattta aattaacccg
      481 ttcgtggtga gcctgtcgaa ccatgaacgg caataagttt accttgaagt catctttgag
      541 agaaaagaag gataattcgg ggaaacagaa aacgatgcag taatggaaaa ggttaacgag
      601 aaaggaaaca atatcggttt ttctgttcta aaagtgagtg ccttaaaaga gcaaaaacca
      661 ctttcaatga gccttaaaac caatatagct tagccgatgt tgcactcaag ttatacctta
      721 tgtcttcgac aaccttgcca ttgcagaaat tcatctatat tctatctctt atgtcaaatg
      781 ttgagacctg accccatttc gcccttaatg tcctgaccca ccccttatat agaataactc
      841 agttccttaa tttggtaaaa gtggattaaa agtcctaccg ctttatttgg tagcccgctg
      901 attctctatc ccaagtatca ggggttatgc cttcgtcgcg gagcttgttt ttctgcactc
      961 tttctgttcc agttttggga aaggaactta caaaccgaat aaaacgggga atcatgaagt
     1021 agggcatttt gggttcgagg aattttaaaa gctcttctgg tgaaacatcc ataccaggat
     1081 ttttcacaac aaaaatcatg acttcatcct ctgcatatcc accggtttga gcctttactc
     1141 caaccgctgc tgattctgca acagcaggat gagaatttac aatcctttca acctcaaatg
     1201 agctaatatt ttcgcctctt cttctgatgt aatcctttac cctgtcaaca aaatatatgt
     1261 aacccttttt atccattctc ccggcatcac cggtatggaa ccagaagttt cgaaaatcct
     1321 gaactgtttt ttccggcatc ttgtggtaat agtgaagcat tatgtttgga attcgggggc
     1381 gtactacaat ttctccaaca cttcctctcg gaagttcttc atctgtttca gggtcaacta
     1441 ttttaatttc gtaacctggt gattcttttc cacatgagcc gactcggaaa ggctcaccag
     1501 gacgcataaa tgtgacctga cctatttctg tgagtccgta tccttccata aacttgatat
     1561 taaacctctc cataaatggc tcaataatgt catgaggagc tggagacccg aggactaatt
     1621 ttactccatg ctcgttttcc tccggcactg gtggagtatt ccacataaaa taagacatag
     1681 taccaaccat attaaacttt gtaaccccat aatccttcat ccatttccag aactgagtgg
     1741 cgctatattt ttcctgtatg attgcccttg cccccattat tagagcagga tacacactca
     1801 tcaccattgc gtttgaatgg aaaagaggaa gacacgtaaa gcaaacatca tccccccata
     1861 catccataac ctcggcgtag tttctcgcgc tttggtaatc cgcctggcag ggtctgacaa
     1921 cgccctttga ccttcccgtt gtcccagatg tgtacatgag ccttgcatga tcaagatgag
     1981 tgattttaac gtcaggctcg tcttcacctc gatcatttat aaactcacta aacggaacca
     2041 tgggttttga atcaaaaccc tgagagtcaa attcagcagc gcctgttcgt gtccatacaa
     2101 taaccctttc aagctgagga attctatcgg caatcggagg cattctgttc agatattctt
     2161 ctgcaacgaa tagcacttta gaatctgaac tatctaagat gtactgaaga aaatccatct
     2221 tgtacgctgt gtttatagga accataatcc cgcccatctt tagaatccca aaccatgtaa
     2281 gaacgtagtc agaagagtta ggcatgtaga cggctacttt ttcacctttt ttaatgccga
     2341 ggtgcataat tccgttcgca attctattgg ctagaagatt gattttggaa aaactaaatg
     2401 gaggattgtc tccaaattgt aaaaaaggtt tgtcactgag cttttctgct ctttccctta
     2461 aaataagagg aagtacccac ttggttctat cttccctctc gtaccacctg acatcaaatg
     2521 gacgacgcct ttctatgttt acagcgtatt tcaggtcctt gggtcttttg gttttaacag
     2581 ccattggtct tttaccttct caaaaaaaca tttttatttt ctactgcaag gattagattg
     2641 tcaaccttcc taatgtctgt ttagctagta ggggcaaccc ttgcggttgc ccaaaataac
     2701 aatgcgggtg caaggcctgc ccctacactg acgtattggt aagcggggtt agaaaacccc
     2761 gactatctaa tgattcatga attgtcacta tatgaatcac ttaaacgcac gtctggttgg
     2821 tacagctaat acctgttcaa gtgtaaggac ttttaggaat ctatttccac cctcttcata
     2881 tatctgatca atccctccga acctcgcagc ttcgagcgcc ttcatcccgc catcagttac
     2941 gtatttctct cctgtaagct ttatcgttat aattccaata tcgcctcttc cattaagtag
     3001 gggcaattca tgaattgccc ctgtaatttc gattcttgcc cttatcttct catgtggacc
     3061 ttctttccca ccggcaaaca gtacctgaac aatatctcct ggataagtcg gagcatcatg
     3121 tgatccgcta ttcattccaa gatgatatgc aagcgggaaa tgtccactta cctctccttc
     3181 tgctcttcct ttcactcctc caccatagcc gatgaagttc gatgatggat tatgatgcac
     3241 agcagcatca tttagggttg caatctgaag ccagctaaat gttactaggt caattgcatc
     3301 ttcaaataat ccatgataaa catcctccac cctaaaatct tcaaatgttt ttccattttt
     3361 tccaaggagt tcttttgata aatcgcgaaa acctttaggt agaacagttt tttttatatc
     3421 aatcttatcc ggttgcgatt ttatagtgct cgctttagga taggtctttc caccctcggc
     3481 tcttacgagc acctgtctca cgtattcaag tactgattct cctctctggt tataaatcgt
     3541 agtctttacc tgtaccgagc cgtttgtcgc accatctttt ctgaattcct ttcccaggac
     3601 ctcagatttt gcgtagatta catcaccctc ataaaccgga acaccaaatc ttaagtcact
     3661 ataagacaga ttagcaatag cattaaatga aatgtcgtgg acactaaggc caaatcctgc
     3721 gttaaaaata aaaccctgac tcacgagagc cgatttctct attccatatt tttccttttt
     3781 caatttgtaa tcaggatcct catatcccag atgtaagctc agctctgggt ttgcccccag
     3841 gatcaaacca tctctatatt tgtttatata ctctctattt ctcattggcg taatttgaac
     3901 cgcgccgtgg tgataaattt caccttcatg aaaatcaccg acatatcttg aagaaatgag
     3961 attcaacccc tgcaatagaa attccctcct tgtatagccg ttttcggatc atatagagac
     4021 gccattaatg gcgtctctac aatattccac aattggttcc tttgtgttaa ttcgtggcta
     4081 aataaaacat ttaaatatta cccaatatca agaaggtggc aattagattc tggaaaggtg
     4141 ttggagctga tcctaaccca gaatatgccg ctactattcc gcaacaagtt tttatggttt
     4201 accaattgat gcttgcaatt atcacccccg ctctgatcac tggtgcgttt gcagagcgtg
     4261 caaaattcaa cacatttatc attttcatcc tactgtgggc aacatttata tatgatccct
     4321 agcacattgg gtatggggag accctgatga gataagaaga aagctacaga tcgttcttag
     4381 aagatttaat cctaaattca ggattgaggt agaggaagag taaacaatcc ttttcgtgtt
     4441 tccttctttg gtaattgaga gtatcgctat cctctcataa ttacactttg ttttaagatt
     4501 cgatcaattc tgtccaatta tctagaaagg taaaacagtt ttatgtttga ttaaatctta
     4561 agcacggtat gtccaattta tgggcataag agttaaaatt aactaacttt tcttaataaa
     4621 ataactggca cactttttgt atatctacta gcttgacttt tcccacactt ctagtctaag
     4681 ggaggtttac agatggtgaa gttaaaaaag ggtacagaca ctacaatttt gggtctaact
     4741 ccggttatta cgctcctaac actagtctta cttacaagtt tatctttcgc tcaagaggcc
     4801 gccccgccaa cggttgacac gggtgacacc gcttggatgc tcacatcctc cgctttggta
     4861 ttgatgatga ccatcccagg tctattccta ttctacggcg gcctggtcag atcaaaaaac
     4921 acacttggca ccgttatgca aagctttatc attgttggcc tgattagcat tcaatgggcg
     4981 ctgtgggggt atagcctggc cttcggacca gacaagggag gtatcattgg aagcctcgct
     5041 tggtttggat taaaaggagt gggacttgat cccaacccag actacgccgc tacgatacca
     5101 caccaggcct ttatgatcta tcaattgatg tttgcgatca tcacccctgc gctaatcacg
     5161 ggtgcatttg ctgagcgtgc gaagtttagc acatttatca tcttcattct gctctgggca
     5221 acatttatat acgaccctct tgctcattgg gtgtggggtg taggtggatg gttaagaaag
     5281 ttaggagcct tggattttgc tggtggaacg gtggtacata tcagttctgg tatatcggct
     5341 cttgctgctg ccattatatt tgggaggcgg attgggtatg gacatgaagc tatgccaccg
     5401 cataacctcc cttttagcat tatcggtgct tcactcctat gggtcggttg gtttggattc
     5461 aatgctggga gtgcgcttgc ggctggcgga ctggctacca gtgcctttgt ggtcacccac
     5521 atggcaacag cggcagccgc gctttcatgg atgtttatgg attggatatt taggggtaag
     5581 cctacggttc taggtgctgc cagtggagct gtggctggac ttgttgcaat cacgccagca
     5641 tcaggctttg tcgggccgat ttcgtcaata atcatcggaa ttggtgcagg tgtcttctgt
     5701 tctatcgcat gcaacgtgaa gccgaaattg ggttatgacg attctcttga tgttgtagga
     5761 gtgcacggga ttggaggaac ctggggagcg ctggccacag gtctatttgc ttcaaaggca
     5821 ataaatgcag ccggaaacga cggcctcttc ttcggtaacc ctgggcaact ctggattcag
     5881 ttcgtcgctg tagcggtaac ctggatcttt gcctttattg ggacactgat aatcctttca
     5941 attctcaagg ttataatggg cctaagggta tcggaggaag aggagcagat gggactggac
     6001 ttgagtcagc acgaggaaaa gagttatacc ttgtaacact acttacttga tagactcgat
     6061 ttagattgat agcagatttt aggatttcgg attaactaaa aaccgaagat ttagaaagag
     6121 agaagtgaaa ggataacacc attcattatc ttttatccat cggaaggtag gttaaatcgt
     6181 taggtccaac gtactcagca acgggtctta ttaaagtatt attgctatat tgctcaagta
     6241 catgagcaat ccatccgctt acgcggctta atgcaaaaat aggggtgtag agatctatcg
     6301 gtatgcccat ggagtaatat gttgaagcag aatagaaatc cacattggga tacatgcctt
     6361 tttcgtttag cataaaatcc tcaatcactc ttgacatctc ataccacttt gtctcaccgg
     6421 ttttctctcc catttcttta gacattcttc taagatgtgt ggccctcggg tcttcagttt
     6481 tatagaccct gtgaccaaag cccatgattc gtttcctttc ggctaacctt gtccttacat
     6541 actctttagc tatttcaaca gtacccatat cagtgagcat tttcattacc tcaatatttg
     6601 ccccgccgtg aagaggtcct ttaagagtgc caacggccga tgtaattgcc gagtataaat
     6661 cagaaagtgt agatgaggtt acccttgctg caaatgtaga agcgttaagc tcgtggtcag
     6721 catgaaggat tagacatata tcaaaaaacc tcgagaccat ctcatctgga actttgccag
     6781 ccaacatgta gagaaaattt gctgcatgat tgagattggg attcggttct atcggttctt
     6841 tgccatttct aattctatca aaggccgcaa taattgttgc ggttttcgct gtaagattaa
     6901 ctgccttttt tacatttgat tcaaacgagt tatcatcagc actacgatca gtcagcgaaa
     6961 gcgtcgaaat ggctgttctt aaaacctcca ttggtgtagc tttcttagga aaggatttca
     7021 gtaaagaaat ggcttctttt gaaagagctc tattaataac aaggctttta tatagatcat
     7081 caaaattctt ctttgtggga agcttaccat accagagtaa ataaacgact tcttcaaagt
     7141 tagaatgctt tactaaatca tgtatatcat aacccctata tataagcttt cctctctttc
     7201 catcaataaa gcagatttca gatttgccaa tcaccacacc ctttagtcct tctgctacta
     7261 tactttctgt ggtcatctta actcctttag gcagttaaat tgaattttaa accaaaatcg
     7321 ttgaattata acacaattta attaagctgt gattacaaaa aagaaatttt acataaatta
     7381 atcaataacc ctgcaacaag gagcccttcg tctagctcag gataaactac gcgacgaagc
     7441 aatctaaaat gagaaattgc ttcacggagc ctgcactgaa cgaaatgaac gtgttcgcaa
     7501 tgacaataaa ctattgtaga aaccctgtag aaaactacag agaattctca aggtaaattt
     7561 aatttctaac gttgaattag gatattttaa atcgcaaata agcattccaa catcttaaaa
     7621 gaatctcgct ttaagaccca tcatgtagag aaggtcgtca agtgtaatta ttccgcttag
     7681 cattcccttt ttgtcaacta tcggcaccct tcggattcca ttttcatgca ttactttcgc
     7741 aagctcagca accctttggt tgtccctccc ggttacaatc ttttttgtca ttacgtcttt
     7801 tacaagtatt gaatttatat ctgctttctt ggctacaact ctaattgcta tatctctatc
     7861 cgtaagaatt cctacaggtt ttctattttc aaccactaca acgctaccaa cattcttttc
     7921 ttccatgagc tttgcagctt tcactacagt gtcatcaggt cttactgtta caactttctt
     7981 cttggtaatc tttcttagag acattttttt acctcctatg cttcgcatat gtagggacag
     8041 cccttgcagt tgccgaaaag caggaatcta cacctatatg gtacagaatg acttacagtt
     8101 gttattaccc cacctcttaa gcgggaatcc ataaacatct gctcatcctg aataaaatgt
     8161 caagggtcgg ggctagaaag tccaacctat cgttgctaaa ttgatcctcc ggggatcctg
     8221 acgtttcttt atctttattt cccctggaga aatcaataca cgcgctttat acataattaa
     8281 tttacccaca aagacctcca aggcacaaag ttttttaagg agttgggaat taggcattag
     8341 gatttggata cccactagtt cccaatcccg aattcctaat tcctaacttc atgccttagt
     8401 cgcagaaagt tatctatttt taaaataagg gattatctgt tttgcatatg ttttaagggc
     8461 tttttccgga tctggactaa aacggtagaa aaggacaaaa tgtctaacgc ctttatcaat
     8521 gtatttttcg atctttttta tgcaatccct tttcgaaccc gtaattgtaa aatccaagac
     8581 cgcttccctt gggaagaact tccctatttc tctaaacttt tgccttcctg cagcatcttg
     8641 agggattaca ttgaaataat tcagtccttt atagtcttcc gggatctcga tatcatatcc
     8701 ggctcttttg agttgttctt gcattatcaa gacatatttg aatggttcga gtgtcttata
     8761 ggcttcatca acattctttc ctagagatgt aaatacccat agaccagggt cgaagttatc
     8821 atctagagtt cgccccacac tctgagcccc ttgtttaaca ttatttaagt actcagcata
     8881 aagaccgggg gttagatcaa ttggaagcca accgtctcca agttcccctg taagtcgcaa
     8941 cccctttgga gtatgagtcg caatatacat agggattttg tctcttttat aaggctttac
     9001 ctgtaggaaa gcttgtgaaa gagtataaaa attcccgtga aaatcaacag ggccttcact
     9061 tttccacagc atttgcataa ccttcatggc ttctctcagt ctagcaagtg gcctatccca
     9121 ctttattcca taagcatcta agttcattga ttctccaacc ccaagtgtta aacaaaccct
     9181 gccttcagag agatggtcta tcgttgcaag acgctgagca aaaacagcag gatgcatcct
     9241 atgaggatca gaaactgttc caagccttat attctttgtt ttcgttgcgg caactgttgc
     9301 aattgtccac gcctcatgcg catctgttgg cgacacaaaa acaacatgat cagggtacca
     9361 tacgctatca aaacccagtt tgtctgcttt cacagaaaaa tttaaaacac catttaaatc
     9421 agcacctggc ataggggctg ataatccgaa ttttatatct tttcccattt ttcacctcac
     9481 aatagatttc ctagtcatta taaacaaatg agtacattaa ttgcaacaca ctaaaaaatg
     9541 taggggcggg acactgacct gcctattttt tggttaccca aattgcaaat catagaatta
     9601 cctgcagggg cggttcaccg aaccgcccat atgcgattcg tctaattact tgtaagcgca
     9661 acctcttggc atgactgttg gcattgtttt aaaccattta acaattttgt gttcgttctg
     9721 tggtattgta ggagcacccc ttggctgtgg cgagcccagc cgagccacgg ttgtccaaaa
     9781 gcgggaatcc attaattata aattcaagac ccgctcctct atccttgtct tttcttcttc
     9841 cgatttcaga attctggctt ccgacttcta attatcccag attacattcg aaaaactctc
     9901 atctcttcac atgtactacc tcaaagctat ttgggagtta ggtcattctg gcttgggaag
     9961 aaatagatgt tataattaac cactttttat ggaggcaaat ggatgaaatt cggaattggt
    10021 ctctttagtt tacaaacaca tcctgaaaac cctagctcgc atgcagagat ctgcagagac
    10081 acgcttacac aagtaaaact cgcagaggag gtcaatttcg attcagcatg gatctcagaa
    10141 catcactttc ttgatgacgg ttattgtcca tcacctgcgg ttatagcctc ggctatggcg
    10201 gcagtaacaa gccgtataaa aatcggttca gccggaataa ttctcccact acataaccca
    10261 gtaagggtag ccgaagactc agccctcgtg gataatattt ctaatggaag atttaatgtt
    10321 ggggttgttc taggctatcg caaggatgaa ttcgatggtt ttgggattcc catgaaacag
    10381 agaccttcac gtatggaaga aggaattgag gtaatagtca aatcatggac tgaagaaaaa
    10441 ttctcttacg aaggaaaacg gttcaagttg agtaatataa acgttacgcc aaaaccggtt
    10501 caaaaaccac atcctccaat ttatatcggc gcatttgagg aacctgcgat aaggcgcgca
    10561 ggaagactgg gttgccccct tctcataggc cctggaagaa caatgcagat ggttagggat
    10621 acactaggat tctacaatga tgcagcccaa aaggctggga ggaatcctaa taatgttgag
    10681 cacatactcc ttagagaagc ttatatcgca gaagataaga aaaaagcaaa aggagaagcc
    10741 gataaataca taataagcat gtataaatac tacttcggct taggagtaaa gatgtttgtt
    10801 cgtggaaagc agctcactgg tttagacgac ccgatgtttg accatctagc agaagaccgt
    10861 tttataatcg gcgatccaga ggattgtata agagaaataa ataggtatag aaacgaactt
    10921 ggaattaatt atataacctg tcgaatggta ttcccccaag caacacacga aacaatatct
    10981 aagtgtatag agctttttgg aaaggaaatc atccccatat taagttaaac tagctcacac
    11041 caacaatctg taattcttcc ttctattttt aaaaatattt tgccttcatt caaatttata
    11101 tcactctgtg tcaaaactta agtcaacagt agcgagtcct tttacacaaa gtccagatag
    11161 tgttcattgc tacatataat aaaacatata aaaacataca agatagatta tcaaaaacag
    11221 cttaatattg tacagtcaca aaccccagct tgacatttcg ttgacattag agttaaattc
    11281 tttttaacct ttaagttctt tcataataaa aagttcttag gtgcccacaa cagggcgtaa
    11341 aagggaaatc cggtgagagt ccggtgcagc cccgctgctg taggtagccc taacccttag
    11401 catgatgcca ctggaatcat aagggattct gggaaggcgc taaggatctt gaaggctacg
    11461 agccagaaga cctacctgaa aactaaactt tactattctt cgagggaaga atgtagaggg
    11521 tccttaattt gaaaaagaac cccagttttc ttcctcgaag actggggttt ttaatgaaga
    11581 agcttttatt catcctaact cttttttatt ttgcttccaa tttactatac cttttcaaac
    11641 cctctttaat tctatttgat aaaagacatg tacaaaataa agcagcaggc aaagaagtat
    11701 ttattgatag agacttttta ttctataact atgcgttcgc tcaagagtca ggaaaaacgc
    11761 tcgaacctat tattgttgaa gagacacctt tgaaaccacc tcttgattac ccatctgcat
    11821 tctcaactgt gattgacctt gaggattttg gtggagaata taatactaca tcagagattc
    11881 tctcattttc gccaggagta tttgtaagag atttcggcgg atttggccaa cttcaaacca
    11941 tttcaatacg tgggtcctcg aacgatcagg ctgtagttct cttagacggt gtaaggttaa
    12001 atacagcgct tggcggaata gacctatcta caatcccttt agattacgtt gagaagtttg
    12061 aaataataag gggtggctca tctgctcttg taggcacgga tgcaataggt ggcgtagtaa
    12121 acatcgtaac aaaaaagact gataagccat ttacctttgg atccgtgaca tatggctcct
    12181 tcaacaccct aagtataaac ctttcaaggg cacaaaaatt taaaaactta agctacttat
    12241 tttcgtacac acacgctcag agcgaaggcg atttcaaatt cgagtcagta aatgacatta
    12301 tacttacaag aatcaataac gaattccttt ctgaaagcgt tcttacaaaa tttgaatatg
    12361 atcttaacgg ttggaaagtc ggttttctga atgaattcta cttcgatgat aagggagtcc
    12421 caggccttgg ggaattccag caacctgact ctaatcaaaa ggacgtaaga aaccttacga
    12481 gcgtaaaatt atcaaaggaa aagtttctaa gagatgactt tgattttgaa atcatctttt
    12541 tcaacagatt tgaccgacta aaatttacta acccagaccc aaccattgga gttcctatag
    12601 atacgttgca aagggacttt gcctttggga ttgacccaaa gatcacatgg tttgcaccgt
    12661 ttaatcaaat cgtgacattt gctactgaaa taaaagaaga gattttaact gataaagatt
    12721 ttggagaacc gaagagattt acttttagcg catttctatc ggatgaaatc cacctttttg
    12781 acgacctatt tatcatcgcc cctatagtga gatacgacct cattataacc tctgctgagg
    12841 aacaggtaaa tacatctcca ggggttatag gaagcccaag agctataacc acaactgaaa
    12901 gcgaattctc cccaaaactc ggaattattg tttcccctat taagcatctc tcgataaagg
    12961 ggaatgtggg aagatccttc agagttccta gctttactga gcttttcttt cctgaggagg
    13021 gtttcatagg tggtaatcct gatcttaaac ctgaaagctc aattgacttt gatgttgggc
    13081 tagttttttc acatccacgt ataggatttg aaattaacta tttcaggaat caaattgacg
    13141 atctgattct ttttgtattt gtaagtgcaa ccagaataga gccactaaat gttggagagg
    13201 taacacaaca agggatcgag actagcgttg tgcttcgacc aattaatttc atagagttgt
    13261 ttgctggata tacgtttctt gatggaaagc tcgaagacac aggggcacag cttccaggta
    13321 gacccaaaaa taagtttgat ctaagagggg tttttaaata cctctttctt aagcttttct
    13381 gggaaaccca cattgtaggg aagattccgc tcagtccttt tcccaactca gccactaccg
    13441 acgcgagcac tgttagtaat gtagggggaa ctgtagagtg gaaacaggtt tttgccacac
    13501 tcgaagcaaa aaacgttttt aacaacttag atgttcaaga tgcattagat tttcctttac
    13561 cgggaagaac aatctttttt actccaggag tgaagtttta acataattat tcagccacga
    13621 ggtgaaacaa atgaacacga ataaaacgac aactaaaaac ttatattttc atgaaatcct
    13681 aagtgactcc gctgcaatta tgtttttctt tttcatagag cttttgtgtg tagcgaaaag
    13741 cttgagcttt ccacggatga atcattttat tagtgtaaat tcgggttcat cagtagccaa
    13801 aagttacaaa ctacaaagga ggatttagct atgaggtcat ctagtaactt acttattttt
    13861 gggattctgg gcatagttct ttggggatgc ggaggaggag gcagtggagg ggagactcag
    13921 caattctttg cattaactaa ttccccagag atagttgtag ggggtataaa aggttctgta
    13981 ggtggcgggg ccatcgttca agtaaggaat ttaagtaatg gacaagagag acaaaccact
    14041 tctagtggag atgggagctt cgttattacg cttgaaggtt caattggtga cacaattgga
    14101 atttcagctc ccggtgttga tactgcagtt gaagtaatgt ctgtctctga tgtaagttca
    14161 aagattaagc aggaaattat gtcaggagca gtgaggagaa atttggcggc aatcgaaggc
    14221 ttcccaacag caattgaaat tcgaggagat agagcctatg tgctcaattt tttcaatcgt
    14281 atagaaatat tcaacttaga ccaggaccca ccgcaaagaa ttggagaaat agttttaccc
    14341 cctgagtctg atccagtgtc aattgctttc ttagatgaca cgtttgctta tgtagtgaac
    14401 aatataggcc agagcatagc tctagtaaat gtagaaacaa tgcaatgtga actccttatt
    14461 gttcgttccg actctgttcc acccgctaca agtatttgcc aaaatcaagt aatcaccgta
    14521 gaccctgagt tttttgagga cccgtccgcc atagcaatag caccaaatgg taaggtttat
    14581 gttacaaaca acaaccttga cgacttcttt gaacctatag gaaatggatt tataactgta
    14641 attagctccg agacaaatca ggttatcaat cgtatagacg caacgagagc taacagtgat
    14701 ggaataacta ttattgataa caggttatat gtcgtgaata ccggagacac ggattttgac
    14761 ctcgttacag aagagaattc ttgtgatttt gattttccgc cgtcaataga cgtgattgat
    14821 actcagtccg ataccagaat tacgatcaac atatctctta gtacccaaaa cccaaatgtt
    14881 tgttctccaa accgtataga accaacacct gacggaaaat ttggttacat ggggttagga
    14941 cttgtgggag ctttgctcaa agtagattta gaaaacaata gcattattaa tggccccagt
    15001 gatccaatta tcataacccc ccttgatgag tttaacttca cggcggatat aaaaatccgg
    15061 aatgacggtc tgggttttat aacgcttttt aacacagacc aaatcgcagt gtttgataca
    15121 gcaactgatg aaataaatcc atttccgttt atagcctttt tccccgcggg attcggggct
    15181 gagaatccag attccgaatt ctttgaaggt gtacagtctc ttgctataag gccaggaatt
    15241 ccaggaattg actttcaggg agcggatata ttctatatta ctagtgttgg acaaagcact
    15301 catctaggct cgattaatac gggacccctt acacctgaat aatttgttct gggtctatat
    15361 tgtggggggc agatctagta tctgcccttg taaagggcaa tccaattaat tgcccctgta
    15421 aattcgcctt gcatctagcc ataaagtagt taaagatatt atcagggaga tggttgttta
    15481 agaggtcaca atgcgtatct gttcatttct tccaagtgca acggaaattg tttatgcgct
    15541 aggattgggt gaaaatctag ttggagcaac tcacgaatgt gattatccac ctgaggcaga
    15601 aagtaaacct agggttatag cgagtttaat aaatcccgaa gatttaacaa gctatgaaat
    15661 tgataaagca gtaagcgaaa actacaaggc tggaaaaagt acatatattg tagacataga
    15721 aacgctacgt agaaccaacc ccgatataat ccttacccaa ggtttatgcg atgtttgtgc
    15781 tgtgtcggga aatgaagtta tagaatcagt gaaggttcta ggacataatc ccaagataat
    15841 ttccctagag ccaaatacgc ttggtgaaat cctaaataca atattactag ttggtgaagc
    15901 cacagaaaga aaggaacagt cagaatattt agtgaataat ctccgatcca gaattgctgc
    15961 aattaaatct cttctcggag aagaaaaaga ccgccccagg gtttattgta tggaatggct
    16021 tgatccttct tacatagccg gtcattgggt acctgaaatg gtacggattg caggcggtga
    16081 ctatggactt ggaaaggaag gcaagccatc ttttaaagtt tcatgggatg agattttcga
    16141 cttcgctcct caggttattg ttctaatgcc ctgtgggttt aatatagaaa gaacccttag
    16201 agaaatcgag attctaactt ctaatgaata ttggcataga ctccctgctg ttaggaaagg
    16261 ccacgtttat atagttgatg cgaactccta tttcagtaga cctggaccaa ggatcgtaga
    16321 tggcttggaa atattagcaa agataataca tcctgaatta tgcacatttg aaattcctgc
    16381 agattctgtg cttaatctta gaaactacat gcaccttgtg gcatttttag gatgatttga
    16441 tatatagttt ctgcaaggga ggaccgaagc cgagagtata gcgaagcaga aggaataccc
    16501 ctcgcctgtc attgcgaggg acgaagtccc gaagcaatct caaaaataat caagagattg
    16561 cttcgctagg ctcgcaatga caaccatggg gaaaaattac attgagttct atgagaaact
    16621 ttatcaaagg atttttacca atttttatac cccttcttat tttattaacg cttctaaagg
    16681 tttcctattc ggagaacttt ccaaggagaa ttgtttctct ttctccaagc ataactgaga
    16741 tcatctatgg actcggagcc tgggaaaatg ttgttggtgt aacaatttac tcagactttc
    16801 caccggaagc tacaaacctt cctaaagttg gcggatgggt taatccaaat cttgaggcta
    16861 tccttgctct aaaacctgat ttggtaataa tgatagaaga ccaagatagg atctttggcg
    16921 ataaaattag gaagataggg ttaaagacac ttgc
//
LOCUS       DATDRL010000054         3824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2819218, whole genome shotgun sequence.
ACCESSION   DATDRL010000054 DATDRL010000000
VERSION     DATDRL010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2819218"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VI935_01900"
     CDS             <1..316
                     /locus_tag="VI935_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HLE24385.1"
                     /translation="RGRIKELLNEIEIDETRLYQEVAILADRSDITEETVRLKAHINK
                     FKETFNKEGSIGRELDFLLQEMNREAGTISAKSKDAEISHLIVELRSEIEKMREQAQN
                     IE"
     gene            complement(449..1951)
                     /locus_tag="VI935_01905"
     CDS             complement(449..1951)
                     /locus_tag="VI935_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HLE24386.1"
                     /translation="MVSRELRQYKEEIEKRAHEYELDFFEVVFEVIDFEEMNMLASYS
                     GFPVRYTHWRFGMEYEKISKSYSYGLHKIYEMVINNDPAYAYLLKGNTDIDQKLVIAH
                     VFGHSDFFKNNFWFSVTNRKMLDEMANHAVRISRYIDKYGHEEVEGFIDICLSIEDLI
                     DIHSPFVKRQFAAKSFLEEDEGERKPVSKLRSPSEYLEDYINPPDFIEWQRRKSEDEK
                     IKKKKFPESPKRDVLLFLLENADLEEWQRDVLSMIREESYYYAPQAQTRIMNEGWAAY
                     WHSKILTEKVVTDTEIIDYADHHAGTLAGRTGSINPYKLGMELFRDIEDRWNKGKFGK
                     EYEECNDIEVKRKWDVKLGLGRDKIFEVRKFYNDVTFIDEFLTEDFCGEHKLFTYAYN
                     RSTNMYEIESRDFKKIKEKLLFLLTNCGRPIIEVTDGNYMNRGELYFIHKHEGVELKL
                     DWAKDVLKHMFRIWKRPVHLETSIDGKVRVFTADGGKKGKEVEVESFLVR"
     gene            complement(1951..3054)
                     /locus_tag="VI935_01910"
     CDS             complement(1951..3054)
                     /locus_tag="VI935_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HLE24387.1"
                     /translation="MSLRIEQDWGRFREIVRGKIKQNLRKYIVRSELIGKKGRDLVSI
                     PVPQIEIPRFIHSLEKSGGVGQGDGDVGTPVGLGEYEGDRQPGNQPGEHILEVEITLE
                     ELARILGEELELPKIEPRGKKNIKSFSGKYSDIHTAGPESLRHAKRTFREALKRQIIT
                     GSYDIVDPVIIPVTEDKRYKAREPVEQPAFNAVVIYMMDVSGSMGSEQKEIVRVESFW
                     IDTWLRYQYKDIETKYIVHDAIAREVDRDTFFRVRESGGTIISSAYKLFRKVTDERYP
                     YGEWNVYAFHFSDGDNWSRDDTHECIEILEESLLPWVNLFCYGQVESPYGSGQFYYDL
                     EDHLGEKENLVLSKIPNKDAVYDSIKKFLAKGR"
     gene            complement(3183..>3824)
                     /locus_tag="VI935_01915"
     CDS             complement(3183..>3824)
                     /locus_tag="VI935_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HLE24388.1"
                     /translation="NPFMVISEIEGGLVHHSLISSEAQRRRFKELLTLVRQEYEEIIK
                     NEVERAIAADEEAIARLCGNYIDNVKAYIRKEKIKNPFTGEDEEPEERLMRSIEEKID
                     IPESRKDDFRREIMNYIGALALEGKRFDYKSNERLRRALELKLFEDQKDSIKLTSLVT
                     NVIDKATQEKIEIVKSRLIRDYGYCESCSSDVLTYVASIFARGDIKEHREHRR"
BASE COUNT         1071 a          904 c          622 g         1227 t
ORIGIN      
        1 aagagggcgt ataaaggaac tcctaaatga gatagagatt gatgagacca ggctttatca
       61 agaggttgct attttagctg atagaagtga catcactgaa gaaaccgtga gacttaaagc
      121 ccatataaat aaatttaaag aaacttttaa taaagagggc tcaattggca gggagctgga
      181 ttttctcctg caggaaatga atcgtgaagc agggacaata tcagcaaaat caaaggatgc
      241 cgagatctca caccttattg tagagctgcg gtctgaaatt gaaaagatga gagagcaagc
      301 acagaatatt gagtgagatg caggatacaa gatttatata aaagcattca tgaattaatt
      361 ccatgcaccg tggatcatga attatgaatc atgaatcatt tcatttagtg ggaatttatg
      421 tttattcgtg gctaaatcgt caaagtaact atcttaccaa aaatgactcg acctcaacct
      481 cttttccttt ctttcctccg tcagcagtaa ataccctaac ttttccatct attgatgtct
      541 caaggtgaac tggacgcttc cagattctaa acatatgctt taatacatcc tttgcccagt
      601 caagttttaa ctccacccct tcatgcttat gaataaaata aagctcacct cgattcatat
      661 agtttccgtc tgtgacctcg attatcggtc ggccgcagtt tgtaagcaga aaaagcagtt
      721 tttcttttat ctttttgaaa tctcggcttt caatctcata catgttagta gacctattat
      781 aagcgtaggt aaaaagctta tgttctccgc agaaatcctc tgtaagaaac tcatcaataa
      841 aagtcacgtc attataaaac tttcttacct cgaatatctt gtctctccca agcccaagct
      901 tcacgtccca ttttcttttt acctcaatgt cattacactc ctcgtactcc tttccaaact
      961 ttcctttgtt ccatctatcc tcgatgtccc taaaaagctc catccctagc ttatatgggt
     1021 taattgagcc agttcttcca gcaagtgttc ctgcgtgatg gtcagcataa tctatgattt
     1081 cagtatcagt aacaaccttc tcagttaaaa tctttgaatg ccagtaagca gcccatcctt
     1141 cattcataat tcttgtttgc gcttgcggtg catagtagta gctttcctct cgaatcatag
     1201 agagtacgtc cctttgccat tcttcaagat ccgcattttc aaggagaaac aaaagcacat
     1261 ctcgtttggg tgattcaggg aatttctttt ttttaatttt ctcatcctcc gatttccttc
     1321 tctgccattc tataaaatca ggaggattga tataatcttc taaatattcg ctcggacttc
     1381 tgagtttaga aactggtttt ctttcacctt catcctcttc aagaaaactc ttagctgcaa
     1441 actgacgttt tacaaatggt gagtgaatat caatgaggtc ttcgattgaa aggcaaatat
     1501 ctataaaccc ctcgacttcc tcgtggccat atttatcaat ataacggctt attcttacag
     1561 cgtggttagc catttcatca agcatttttc tgttggtaac agaaaaccaa aagttatttt
     1621 taaaaaaatc agaatgaccg aagacatggg ctattacgag cttctggtct atatccgtgt
     1681 tacccttaag caaataggca taagctggat cattgtttat taccatttca taaatcttat
     1741 ggagcccata tgaatagctt tttgaaatct tttcatactc cattccgaaa cgccagtgcg
     1801 tatacctcac agggaaaccg ctgtatgagg cgagcatatt catctcttca aaatcaatca
     1861 cctcaaaaac aacctcaaaa aaatcaagct cgtactcatg tgcgcgcttt tctatttcct
     1921 ctttgtactg tcgaagctct cgagaaacca ttacctaccc tttgcaagaa acttctttat
     1981 cgagtcatac actgcatcct tgtttgggat tttagatagc acaaggtttt ctttttcccc
     2041 aagatgatcc tccaaatcat aatagaactg accgcttcca tatggactct caacctgtcc
     2101 gtagcagaaa agattaaccc aaggtaaaag gctttcctcc aatatctcaa tacactcatg
     2161 agtatcatca cgggaccagt tgtctccgtc agagaagtga aaggcataaa cgttccactc
     2221 accataggga tagcgctcat ctgttacttt ccgaaaaagc ttatatgcag aggaaattat
     2281 agtgcctcca ctttctctaa cacgaaaaaa agtgtcacgg tcaacctcgc gtgctattgc
     2341 atcatgcaca atatattttg tctctatatc tttgtattga tacctaagcc aagtatcgat
     2401 ccaaaatgat tcaaccctta caatttcctt ctgctccgat cccattgaac cgctaacatc
     2461 catcatataa atgacgacag cattaaaagc aggctgctca acaggttctc tagctttgta
     2521 acgtttatct tctgtgacag ggataattac tgggtctaca atgtcataag agcctgtgat
     2581 aatctgacgc tttagcgcct ccctgaaggt acgtttagcg tgcctcaggg attcagggcc
     2641 ggctgtgtga atatccgagt attttcccga aaaggatttg atattctttt tgccacgtgg
     2701 ctctatttta ggaagttcaa gctcctctcc taaaattctt gcgagttcct caagtgtaat
     2761 ctcaacttca agaatgtgct ctcctggttg attgcctggt tgtctatctc cttcatactc
     2821 gcctaatcca acaggcgttc ctacatctcc atcgccctga cccactcctc ccgatttctc
     2881 aagggaatga ataaaacgag gaatctcaat ctgtggtaca ggtatactaa caaggtccct
     2941 tcccttcttc ccgattaact cagatcgtac aatatacttt ctaagattct gtttaatctt
     3001 cccccttacg atctctctaa atcgccccca gtcctgttca attcgtagag acacctatac
     3061 ctccatattg caaacaaatt ccaaatctca agcaaatttc aaattcctga ttccaaattc
     3121 ccaactccta attccaaaag tttgtgattt gagatttgtt attcgggatt tgcttttcta
     3181 gtctatctcc tatgctctct atgttctttt atatcccctc gtgcaaatat gcttgctacg
     3241 tatgtgagaa catccgatga gcaggattca cagtatccgt agtcacgaat taatctactc
     3301 tttacgatct ctatcttttc ctgtgttgcc ttatcaataa cgttagttac aagactcgtc
     3361 agcttaatcg agtccttttg gtcctcaaat agcttgagtt ccagtgctcg cctaagtctc
     3421 tcgttagatt tatagtcaaa cctctttcct tcaagagcca gtgcaccgat atagttcatt
     3481 atctcccttc taaaatcatc ctttctcgac tcaggaatat ctatcttctc ttcaattgaa
     3541 cgcataagcc gctcctctgg ttcctcgtct tcgcccgtga acggattctt aatcttctcc
     3601 tttcttatat aggcctttac attgtcaata tagtttccac agagccttgc aatcgcttct
     3661 tcatcagcgg caatagcgcg ctctacctca tttttaatta tttcctcata ctcctgtctc
     3721 actaaagtaa gaagctcttt aaatcttctt ctctgagcct cacttgatat aagagagtga
     3781 tgaacaagac cgccttcaat ctcacttata accatgaatg gatt
//
LOCUS       DATDRL010000055         4472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3078706, whole genome shotgun sequence.
ACCESSION   DATDRL010000055 DATDRL010000000
VERSION     DATDRL010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3078706"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(326..676)
                     /locus_tag="VI935_01920"
     CDS             complement(326..676)
                     /locus_tag="VI935_01920"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HLE24389.1"
                     /translation="MKKRVLIPTVVGLVIGTMLAVSVTLARDTEHERDPGLSCSVTVP
                     DPAPTNLASLAKITAEEAKVKALTGQQDATTVIEVELENEDGCLVYGVELSNGFEVNI
                     DAGNGKVLQVEPDD"
     gene            complement(719..1027)
                     /locus_tag="VI935_01925"
     CDS             complement(719..1027)
                     /locus_tag="VI935_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24390.1"
                     /translation="MKYKVFSFLFSHSFLVRIEYSQYIIDAYMDFKIHDPSPVSQLPP
                     GFAIDDTQPQRQRTQEGALQKEHYSGKVIYLWPTAAGRSTTRRQPRRLRSPICSTPRL
                     "
     gene            complement(1173..1913)
                     /locus_tag="VI935_01930"
     CDS             complement(1173..1913)
                     /locus_tag="VI935_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24391.1"
                     /translation="MKRIVEMRKSMRLLALFLVAVVVLGLAAASRESWAAPFSAAKII
                     FEVNATDGDAGIQVFLDGEPWKNVKIFNPDKVQIAEFRAQGGPMNLEGFGLTELFSES
                     NEPPFEDFPLQDVLDLFLEGKYEFKGTTVGGASLKSIATLSHVIPCGPEIVLEDPLPF
                     DEAIIKWTPVTNEFDPATEECGESTDVDIVGYQVIVGTFQVTLPASATQVTVPPEFLE
                     PNTVYLFEVLAIEKSGNQTITEGSFETE"
     gene            complement(2111..2878)
                     /locus_tag="VI935_01935"
     CDS             complement(2111..2878)
                     /locus_tag="VI935_01935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLE24392.1"
                     /translation="MKLEDKITLITGGGTGIGKATALLFAREGAFVAVAGRRENKLGD
                     TVSIIHKNGGKAIHVVGDISKVSDTERIVKETVHKFGGLDILVNNAGVFKGIKITDTK
                     EEEYDYIMDINLKGTFFMCKYAIPEIQKRDGGAIINIGTALGIKGYRGSSTSAYSASK
                     AGVIMLTKALALELAPHKIRVNCVCPAVVETEIFETLGIPKEKIPERMKMWEAFHPIG
                     RNGRPEDIARAALYFASEDSSWATGSIFTLDGGVTAG"
     gene            complement(3067..3873)
                     /locus_tag="VI935_01940"
     CDS             complement(3067..3873)
                     /locus_tag="VI935_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLE24393.1"
                     /translation="MPKVKVNDININYEKQGTGEPLILIPFLSADQACYAFQVPEYSK
                     HLTCYSVDPRGAGETDKPGGNYTTELFADDIAGFMQTVGIEKAHIAGVSLGAAIGMWL
                     AAKYPEKVKSLSLHSGWTKSDPFLKTVVEGWQVMAKALGSVPEMVIKGIFPWCFTPEL
                     YAARPDYLNALSDFVRGRPAQPVDAFIRQSNAVIAHDCESQLGKIKAPTQITFGRHDM
                     VTSTRFADRMKNNIKNSELVVFEGCSHAGLYEKTEEFNQRTLEFLKQNSK"
     gene            4119..>4472
                     /locus_tag="VI935_01945"
     CDS             4119..>4472
                     /locus_tag="VI935_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24394.1"
                     /translation="MDSRLYKKALLLSYFTVGYNFLEGVVSILAGSLAGSIALVGFGL
                     DSFVESLSGGVMVWRLSKHGEISLEEEEKIEAKAIRLIAYTFFILGAYVLYESIKKLY
                     FREIPDPSLLGIIIAT"
BASE COUNT         1171 a         1063 c          892 g         1346 t
ORIGIN      
        1 catcctccac caccactgtt gttgcaccca accccccaga ggataaggaa aagagaaaga
       61 catatggcaa atagatgaat acatcgtttc atggttatta ttttcttcat tttttcccct
      121 cattatgaat ttattcttta agtgaagtta acacaggaag attaccggaa cctttcctga
      181 acattacatt ttggaaaggt ttataaaatg tgaagagggg atgaatcaga agctaaagtc
      241 agcggtgaaa attaaacatt aactttcacc gcccggataa ttaatcttct aactcagatt
      301 tttcgctatc tactgcttcg ttcttttagt cgtcaggctc tacctggagc acctttccat
      361 tgccggcgtc tatatttact tcaaacccgt tgctcagttc tactccataa accaggcaac
      421 catcctcgtt ttcaagctcc acttcgataa ccgtcgtggc atcctgctgg ccggtcagag
      481 ctttgacctt agcctcttca gccgtgattt tagccagcga agcaaggttt gtaggcgcag
      541 gatcgggcac agttacactg caactcaatc cgggatcgcg ctcatgctcc gtatcgcggg
      601 ctagagtaac ggacactgcc aacatagtgc caataacgag cccgacaaca gttgggatta
      661 acactcgctt tttcattatt tttacccccc ttacactgaa tttgttatgt aacttttatc
      721 atagtcgtgg tgttgagcag ataggtgatc tcagtctcct cggctgcctt cttgtcgtgc
      781 ttcttcctgc cgccgtcggc caaagataaa tcactttgcc actgtagtgc tctttttgca
      841 gggccccctc ctgcgttctt tgacgctgag gttgtgtgtc atctattgca aaaccaggag
      901 gaagttggga gacaggactt ggatcgtgga ttttaaaatc catgtatgca tctattatat
      961 attgtgaata ttcaatccga accagaaagc tgtgagaaaa gagaaaagaa aacaccttat
     1021 atttcaagac ctccatccgt ttttgcgcgt ttttgttcgc ttttgttttg gggccacaca
     1081 aagggtggga ctcccttcgc ctcattcact acaacaatac gggatgtgga tggaatccca
     1141 gcgaccccat ccacgcagat tttgtagggg gactattccg tctcgaagga gccctccgtg
     1201 attgtctggt ttccgctctt ctctatcgct aacacctcga ataagtacac cgtatttggc
     1261 tcgaggaatt ccgggggaac cgtcacctga gtggccgagg ccgggagggt gacctgaaac
     1321 gtgccgacga tgacctggta gccgacaata tctacatctg tggactctcc acattcttca
     1381 gtagcaggat cgaactcatt tgtaacggga gtccatttga ttattgcctc gtcaaagggt
     1441 agtggatctt ccaagacaat ttcgggccca catggaataa cgtgggagag ggtcgcaatg
     1501 ctcttcagac tggctccccc aaccgtcgtg cccttgaatt cgtatttccc ctctaggaac
     1561 aggtccaaga cgtcttggag cgggaaatct tcgaacggcg gttcattgct ctcggagaac
     1621 agctccgtaa ggccgaaccc ctccaggttc attggacccc cttgagcccg gaactcggcg
     1681 atctgcacct tgtccgggtt gaaaatcttc acattcttcc agggttctcc atctaagaag
     1741 acctggatgc ccgcatcccc atccgtggcg ttgacctcga agataatctt cgcagccgag
     1801 aagggtgcgg cccaactctc ccgggaagcg gccgccaaac cgaggacgac taccgccacc
     1861 agaaaaaggg ccaacagcct catgcttttt ctcatttcca ctatacgttt catactgcac
     1921 ctcctgaatt tatttttatt ataaagataa cacagcaaga ttacgggaac ctttcctgaa
     1981 cattacaatt tcgtaatgtt tgaacgaggg agatatgatc cccattttat gcaagaatct
     2041 tttacattgt cattatttca ctaagatgag cttaatgttc agatagaaag agtcgtcatt
     2101 aggtcgggaa ttagcctgca gtaacacctc catcaagggt aaatatagat cccgtagccc
     2161 atgatgaatc ttctgatgcg aagtaaagcg ctgcccttgc aatatcctct ggccttccat
     2221 ttctcccaat cggatgaaat gcctcccaca tcttcatacg ttcggggatc ttttccttcg
     2281 gaattccaag agtttcaaat atctctgtct ctaccactgc aggacaaaca caatttaccc
     2341 taattttatg cggggcaagc tcaagtgcta gtgcctttgt aagcataatc acacctgctt
     2401 ttgaggctga ataggctgac gttgaagacc ctctatatcc ctttattccg agtgcagttc
     2461 caatatttat aattgcgcct ccatctcttt tttgaatctc tggaattgcg tacttacaca
     2521 taaagaatgt tcccttgaga ttaatatcca taatgtaatc gtattcctct tctttggtat
     2581 cagttatttt aatcccttta aaaacgcctg cgttatttac gagaatatcc aagcctccaa
     2641 acttatggac tgtctctttt acaatccttt ccgtgtcgct tacctttgat atatcgccga
     2701 cgacgtggat agccttaccc ccatttttat gaattatact aacagtatct ccaagcttgt
     2761 tttcccttct tccggctact gctacaaagg ctccttccct tgcaaaaaga agagctgtag
     2821 ctttccctat ccccgttccc ccgcctgtaa taagggtgat tttatcttct agtttcatct
     2881 tctatataag gttgtaagtg gtttgtagtt cgtgacgccg aattatattt ctcagtttca
     2941 agaaatccgt tttgaggaag aagaaaagtc attgcgagcg aggtaggagt gatttgtgaa
     3001 tcgctactac tattgaaatc gaatctatta cccaatccca aatcccaagc cacgaatcaa
     3061 gaacttttat ttagaattct gtttcaaaaa ctctaatgtc ctctggttaa actcctctgt
     3121 cttctcgtag agccccgcat gagaacagcc ctcaaagacc actaactcgg aatttttaat
     3181 gttattcttc atcctgtcag caaagcgggt tgaggttacc atgtcgtgcc taccaaaagt
     3241 aatctgagta ggtgccttta tcttacccaa ctgagactca cagtcgtgag cgattacggc
     3301 attggattgt cttataaatg cgtccacagg ctgtgccggt cttcccctta caaaatcaga
     3361 cagtgcattt aggtaatcag gcctagcagc atagagttca ggagtaaagc accacggaaa
     3421 aatacccttt ataaccatct ctggaacgct tcctagggct ttagccatca cctgccagcc
     3481 ttccacaacg gttttaagaa agggatcact ctttgtccaa ccactatgga gtgatagcga
     3541 cttaaccttc tctggatact ttgccgcaag ccacatgcca atagctgcgc ccaatgaaac
     3601 acctgctata tgggcttttt ctattcctac tgtctgcatg aagcctgcga tatcatcagc
     3661 aaatagctct gtagtgtaat tacctcctgg tttatcagtt tcaccagctc ctcttggatc
     3721 tacggagtaa caggtaaggt gctttgagta ctctggaacc tgaaaagcat aacaagcctg
     3781 atcagcactt aaaaaaggta taagaataag aggttcacct gttccctgtt tctcataatt
     3841 tatatttata tcatttactt ttaccttagg catttgtttc ccctcctcgt cactggataa
     3901 ttttcattcc tatttatata taggcttacc atataacaaa catggtttct tgtcaacgaa
     3961 aacctaatta gagctgctaa gctttgttgc ctggtaaaaa gaacctggat tttgtttttg
     4021 taaaatacaa cactaatttc agcttccgcg cttagaatat taaatcatct ctttttttat
     4081 ctgaagaaat cgtcaaaata ttaatggaga ttgacattat ggattcaaga ctctacaaaa
     4141 aggcactttt actgtcgtat ttcacagtgg gatacaattt cttagaagga gtcgtatcaa
     4201 ttttagcggg ttcattagct ggtagtattg ctttggttgg atttggcctt gacagctttg
     4261 ttgaatccct ttcagggggt gtaatggttt ggcggttaag taaacatggc gaaatatcac
     4321 ttgaggaaga ggaaaaaata gaggcaaaag ctataagatt gatagcttat acctttttta
     4381 tccttggagc atatgtttta tatgaatcta tcaagaaatt gtactttcgc gagattccag
     4441 atccgagtct tttaggtata attatcgcta ct
//
LOCUS       DATDRL010000056         4699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_707406, whole genome shotgun sequence.
ACCESSION   DATDRL010000056 DATDRL010000000
VERSION     DATDRL010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707406"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VI935_01950"
     CDS             <1..341
                     /locus_tag="VI935_01950"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HLE24395.1"
                     /translation="SAPSFGPIRTGKTLWEVYQVIEDRKRNPREGSYVSGLFKKGLDT
                     ILKKVGEEASETIVAGKNKSKEEVIYEIADLWFHSIVMLVHLGIKLEDIYQELGKRFG
                     KSKESYRRGL"
     gene            complement(407..670)
                     /locus_tag="VI935_01955"
     CDS             complement(407..670)
                     /locus_tag="VI935_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HLE24396.1"
                     /translation="MPLYEYECLKCKHKFSEIIYKKDEEKKLKCPKCKSKKWERLFSS
                     FAVVADLSTNMPKPDLSNLPPEVRSKTMIGDYIEEKDRPKKNR"
     gene            complement(681..1193)
                     /locus_tag="VI935_01960"
     CDS             complement(681..1193)
                     /locus_tag="VI935_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24397.1"
                     /translation="MPVFEYECDRCIDEYNSDISSLVEKLNKSNATKIMKKNPKLCYV
                     EVYDEKKRKLTFAVGEKGKYNKYNIRRFRYNLKNNKILSIELRNFKFSELIYDKEDEK
                     KLKCPLCHKGDKVRRVYSTFKAILDDKNKRAPRPGDELAYHFDYKKKMDDEIKSSWVG
                     YDYLNQYFND"
     gene            1582..2379
                     /locus_tag="VI935_01965"
     CDS             1582..2379
                     /locus_tag="VI935_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLE24398.1"
                     /translation="MTNAKELIIEEKDSVCTITINRPEKRNSLTPEILLEISSELKKI
                     ATEEKTRCLVIRGSGDKAFCSGYDIAAIGKIKDDMMRDYRGDHPLIIASNAIENFPYP
                     VIAMINGHAFGAGLEIALTCDIRISVDNALFGMPPAKLGVIYPYSGVRRFLNLIGVGY
                     TKELFLVGRNIDAQMAQKMGLVNQILPKTDLEEFTYKLAHEICENAPLSVMTLKEMIN
                     AWQRNQSLSTHDEETIKEIILRVQESEDYKEGQRAFSEKRKPKFQGR"
     gene            2437..2805
                     /locus_tag="VI935_01970"
                     /pseudo
     CDS             2437..2805
                     /locus_tag="VI935_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015153928.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
     gene            complement(2815..3321)
                     /locus_tag="VI935_01975"
     CDS             complement(2815..3321)
                     /locus_tag="VI935_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HLE24399.1"
                     /translation="MDTFECIRKRRDIRSYLKKDVSDDVIGKIVEAGRLAPSAMNLQP
                     WHFIVIKNKETLKELGKFCISGRFIVDAFFAIVVITEPSNKWHEIDGARAVQNMSLAA
                     WNEGVGTCWIGAIDRDKVKEKLHIPKNLHVLTILPFGYPEEFTVKRKKVRKLPEEVFH
                     WGKFGKKL"
     gene            complement(3447..4355)
                     /gene="era"
                     /locus_tag="VI935_01980"
     CDS             complement(3447..4355)
                     /gene="era"
                     /locus_tag="VI935_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HLE24400.1"
                     /translation="MEQEIKAKEDFRSGFITIIGRPNVGKSTLLNVIVGEKIAAVSEK
                     PSTTRNRILGIKTLPDAQLIFLDTPGIHKAKDELGKVMVRTAIGAIGEADVILMMIEV
                     DEPFGKGDCFIIDSLPKPAILVINKIDTVKKSEILNIIAESQKFEGKFLEVIPISALK
                     SDGIDDLINTIIKYLPVGPKYFPDDMITDQPERFLTAELIREKIFNLTREEIPYKTAV
                     VVEEFKDLPEKNLVRISALIYVERDSHKGIIIGEAGKMLKQIGSLARLEMERILGTKV
                     YLELWVRVKERWTERASLIKEFGYGS"
     gene            complement(4365..>4699)
                     /locus_tag="VI935_01985"
     CDS             complement(4365..>4699)
                     /locus_tag="VI935_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLE24401.1"
                     /translation="MPNHIHGIIIVGATLAVAQAREKRAGARPAPTIGEIIGIFKSLC
                     LHDWLKYIIENRIDAVGKFWQRNYYEHVIRNEDELNQIRKYIAENPLKWERDEENPIN
                     IKSTTKRQ"
BASE COUNT         1382 a          965 c          844 g         1508 t
ORIGIN      
        1 ctagtgcccc atcatttgga cccataagaa ctggtaaaac cttatgggag gtgtatcaag
       61 ttatcgaaga tagaaaacga aaccctcgag aaggctctta tgtaagcggt ctctttaaaa
      121 agggactcga taccatactc aagaaagttg gagaggaagc tagtgaaacc atagtagcag
      181 ggaagaataa aagtaaagaa gaggttattt atgaaatagc ggacttatgg tttcattcaa
      241 tagtaatgct tgttcacctt ggaataaaac ttgaagatat atatcaagag ctcggtaaga
      301 ggtttggaaa atctaaagaa tcgtataggc gtggtttgtg actcgtgatt agtggttcgt
      361 ggtttcagta cacgattcac gagccgcgat tcacctgtca cgacggttat ctatttttct
      421 ttggcctgtc cttctcctca atgtagtcgc ctatcattgt ctttgacctt acttcaggtg
      481 gcaaatttga aagatcaggc ttaggcatgt tagtagataa gtctgctacc acagcaaagg
      541 aagaaaaaag tcgctcccat ttctttgatt tgcattttgg acatttgagc ttcttttctt
      601 cgtccttttt gtaaattatc tcagagaatt tgtgcttaca ctttaaacac tcatattcat
      661 acaaaggcat taatttcccc ctaatcgttg aaatattgat tcaaataatc gtatccaacc
      721 caggaactct tgatttcatc atccatcttt ttcttataat cgaaatgata tgcaagctca
      781 tcacccggac gcggtgccct tttatttttg tcatcaagaa tcgctttaaa tgttgaatag
      841 accctacgaa ctttatcacc tttatgacac aaagggcatt tcagtttttt ttcatcctct
      901 ttgtcatata tcagctcgct gaacttaaaa tttcttaatt caatagagag gattttatta
      961 ttctttaggt tataacgaaa tcttctgata ttatatttat tatatttccc cttctcaccc
     1021 actgcgaacg ttagcttacg ctttttctca tcataaacct cgacataaca taatttcggg
     1081 ttttttttca taatctttgt tgcattggat ttattaagct tctcaactaa agaagatata
     1141 tcagaattat attcgtctat acacctatcg cactcatatt caaaaacagg catagttttt
     1201 cctcctaatt tacaccttta gaatattaac aaaaaattct tagtttttcc agtggtagag
     1261 acataccatg ccatgtctct actaaattaa aaagatcatt atggatgcac gttaaacatt
     1321 acatttctgc tattggattt gaaaaaattg agcgctatat tagtacgaat ggttttctaa
     1381 ccctgcctga taggacagct atgttggata gacgggactg gaaagtccag tctcggtgta
     1441 ttaaacctcc atccatgata ggcggttttt tccaacaccg cctccaaatg ctggatgtta
     1501 ccaccatgca ttttcaaaaa tttgacaaag attggcatgg gataatacta atttcacact
     1561 tgctttcaag gaggaggtcc cgtgacaaat gccaaggaac tgataattga agaaaaggat
     1621 tcagtctgca ctatcactat aaataggcct gaaaaacgga attccctaac accagaaatt
     1681 cttcttgaaa ttagttcaga attaaaaaaa atagcaactg aagagaaaac aagatgccta
     1741 gtaattcggg gctcaggaga taaggctttc tgttcgggat atgatatagc agcaatcgga
     1801 aaaataaaag acgatatgat gagggattat agaggcgacc accctcttat catagcgtca
     1861 aatgcaattg aaaacttccc ctaccctgtt attgcaatga taaatggtca tgcgtttggc
     1921 gcaggactcg agattgcctt aacctgtgat attagaatat cagttgataa tgccctattc
     1981 ggtatgccgc ccgcaaaact tggagtcatt tatccatatt caggagtgag aagattcctt
     2041 aaccttattg gggttggtta tacaaaagag cttttcctcg taggaaggaa tattgatgcc
     2101 caaatggctc aaaaaatggg cctagtaaac cagatactac caaaaactga tttagaagag
     2161 ttcacatata agctcgctca tgaaatctgt gaaaatgctc cgctgtcagt aatgacacta
     2221 aaagagatga ttaatgcctg gcaaagaaat caatcattaa gcactcatga tgaagaaaca
     2281 attaaagaga ttatcttaag ggtgcaagaa agtgaagatt ataaagaggg tcaaagggct
     2341 ttttcagaga agagaaagcc aaagtttcaa gggagatgaa gataaggagt cagaagtcag
     2401 aatccagaag ttagaatttt gttgggagta tgctagatgg agagagtagc agcaaaaaca
     2461 ttttaggact tagtagtttg gaagaaggcg catgaatttg tgcttgcaat gtataggcac
     2521 acagcatcct ttccaaagca tgaaatttat ggacttacca ttcaaatgcg ccgagctgcg
     2581 atctctatcc cagctaacat tgcagagggg tttaagaaaa gaagcaaatc agataaagcc
     2641 cgattcatga atattgccct aggctctatt gaggaatgtc gttactacct tattttgtcg
     2701 aaagatttgg gttatgaaga tactggagaa ttgatgaata ctttgcaaga agtcagtcgt
     2761 ttattggaag cctatacaaa atcaattctg gcttctatct cctgacttct gaattcatag
     2821 tttttttcca aattttcccc aatgaaacac ctcctctggt aatttcctaa ccttttttct
     2881 ttttactgta aattcctcgg ggtagccaaa tggaaggatt gtaagaacat gtagattttt
     2941 tggaatgtgg agcttctcct ttaccttatc tctatcaatt gcaccaatcc agcaagtccc
     3001 aaccccttca ttccatgcag cgagggacat attctgcaca gctcttgctc catctatctc
     3061 atgccattta ttggagggtt cagtaattac tacgatagca aaaaaggcat caacaataaa
     3121 cctaccactg atacagaatt tgccgagttc ttttagtgtc tccttatttt ttatcacgat
     3181 gaaatgccaa ggctgtaggt tcatcgcact tggtgctagt cttcccgctt caactatctt
     3241 gcctatcaca tcatccgata catctttttt aagataactc cttatatccc ttctttttct
     3301 tatacattca aacgtgtcca tcagatctcc tctctaattt tgattttttc accactgact
     3361 tctggattcc ggctcctaac tccctatggc ctttgcatct tgtgtcatgt atctcgcatc
     3421 ctgcatcatg tatctatcat cctaaattag ctaccgtaac caaactcctt tataagactc
     3481 gccctctcgg tccatctttc ttttaccctc acccagagct caagatagac ctttgtacct
     3541 aaaatccttt ccatctcaag ccttgcaagg cttccgattt gctttagcat cttaccagcc
     3601 tctccaatta taatcccttt atgactatcc ctttcaacgt aaattaaagc tgaaatccgt
     3661 actaaatttt tctcagggag atctttaaac tcctcaacaa caactgcagt tttgtatgga
     3721 atctcctcgc gtgtgaggtt gaaaatcttt tctcttatga gctcggcggt aagaaatctt
     3781 tctggctggt cggtaatcat atcgtctgga aaatatttgg gacctacagg aagatacttt
     3841 ataatagtgt ttataagatc atcaatccca tcagatttaa gagcggaaat gggtatcact
     3901 tctaagaatt tcccttcgaa tttctgcgat tctgcaataa tatttaagat ttcacttttc
     3961 tttacagtat caattttatt aattaccagg atggccggtt taggaaggct atctattata
     4021 aagcagtcgc ccttgccaaa tggctcatcc acttcgatca tcattaaaat aacatccgcc
     4081 tctccgattg cacctatcgc agtccggacc ataacctttc caagttcgtc cttggctttg
     4141 tgtataccag gtgtatcgag aaagataagc tgagcatcag gaagggtttt tataccgagt
     4201 atccgattcc tagtcgtact tggcttctct gaaaccgcag caatcttctc acccacaata
     4261 acgttgagaa gggttgattt cccaacgttt ggtcttccga ttattgtaat gaaaccagag
     4321 cgaaaatcct ctttagcctt tatttcctgt tccattttag cctttcattg tcttttcgtg
     4381 gtggatttta tatttatggg attttcttcg tctctttccc attttaaggg attttcggca
     4441 atatatttac gaatctgatt taattcatct tcgttacgga taacgtgttc gtaataattt
     4501 cgttgccaga atttaccaac cgcatcaatt ctgttttcta ttatgtattt taaccaatca
     4561 tgtagacata atgatttaaa tattccaata atttccccaa ttgtaggggc gggtcttgca
     4621 cccgcccttt tctcccgggc ctgggcaacc gcaagggttg cccctacaat tattatccca
     4681 tgtatatgat ttggcataa
//
LOCUS       DATDRL010000057         2077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3678767, whole genome shotgun sequence.
ACCESSION   DATDRL010000057 DATDRL010000000
VERSION     DATDRL010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3678767"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VI935_01990"
     CDS             <1..313
                     /locus_tag="VI935_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HLE24402.1"
                     /translation="VTDKFPKEEKFSLADQIHRSAISIPSNIAEDAARNTKKEFVNFL
                     HIAQGSLSELDTQIELAKRLKYIGEQSWKELDITMERIDKMLTGLIRSQKSPNASRSH
                     P"
     gene            complement(325..1239)
                     /locus_tag="VI935_01995"
     CDS             complement(325..1239)
                     /locus_tag="VI935_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLE24403.1"
                     /translation="MLVRIIPYLSLIVLIFCACYLGKSVNSKTDENIWKDPQNTLKIW
                     LLSDIQPKNQGHKKQFQKAINDINKNVPDIDFAIVAGDIVQQANEEDFDWYLSTKPSS
                     YIKEWYEIAGNHDVKRDNGELYKKKINEIFNYSFVKGNMLFIFMSDEITSSETDISDE
                     TFNWWRDLVINNQDKIIVTVTHAPLEGSRILFSSFRRRQIKDSKRFTEVLEKYRVDIW
                     LSGHVHVPQWFPNVVGKSEKHNGTMFINVAGIRPEALGLKQSESRIMTFECGSDKVVL
                     KSRNHTEREYNKELETVFEVSKEFKCES"
     gene            complement(1696..>2077)
                     /locus_tag="VI935_02000"
     CDS             complement(1696..>2077)
                     /locus_tag="VI935_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24404.1"
                     /translation="QVNRLCLFHLIITLSKWKEFYDRYRDVIPEDVRDVAKTLSKVIE
                     AKGILDFRNKVVGHVWDEDARRALTATEIEQRLKVVLHPGLKEFMTWVNDPNDNLFPR
                     TAVSVIERVRDRIRELHSFTDAET"
BASE COUNT          581 a          466 c          397 g          633 t
ORIGIN      
        1 ggtaaccgac aaatttccta aagaagaaaa attctcatta gctgatcaaa ttcatagatc
       61 tgcaattagt atacctagca atattgctga agatgctgca agaaatacta aaaaggaatt
      121 cgtcaatttt cttcacatag ctcaaggatc tcttagtgaa ttagatactc agatagaatt
      181 agcaaagcgg ttgaaatata ttggagaaca aagttggaag gaactcgata ttacgatgga
      241 aaggattgat aaaatgctta ccggtctcat tcgctctcaa aaatccccta acgcctcacg
      301 ctctcacccc taacgcctta cgccttacga ctcgcactta aattccttgg aaacctcaaa
      361 tacagtttca agttctttat tatactccct ttccgtatga ttacgtgatt tcaagactac
      421 cttatctgaa ccacattcaa atgtcataat tcgtgattcg gattgcttaa gcccaagtgc
      481 ttcaggacgg atcccagcga catttataaa catagttcca ttgtgctttt ccgactttcc
      541 cacaacattc ggaaaccatt gaggcacgtg aacatgacct gaaagccaga tgtctactct
      601 gtatttttca agtacctcag taaatcgttt ggagtccttg atctgccttc ttctaaatga
      661 agaaaatagt attctacttc cttcgagtgg agcatgagta actgtaacaa ttatcttatc
      721 ctgattgttt attactaaat ccctccacca attgaatgtc tcgtccgata tatctgtttc
      781 tgaacttgtg atttcatccg acataaaaat aaaaagcata tttcctttaa caaatgaata
      841 attaaatatc tcatttattt tctttttgta aagctcgccg ttatcccgct ttacatcgtg
      901 gttaccagca atctcatacc attctttgat ataagatgaa ggtttcgtcg aaaggtacca
      961 atcaaagtcc tcttcatttg cctgctgaac aatatcccca gcaactatag caaaatctat
     1021 atcaggaaca ttcttattaa tatcgttaat cgccttctgg aattgtttct tgtgcccttg
     1081 gtttttaggt tgaatgtctg aaagcaacca gatttttagt gtattttgag ggtccttcca
     1141 tatattctca tcagtttttg aattaaccga tttacccaaa taacatgcac aaaaaattag
     1201 aacaataaga gacagataag gaattattct aacgagcatt gttaataact tttgttcctt
     1261 aaaatcttca agcttatccc acgtcctacg ttcaaactct ttctcgccgt ccctacatgt
     1321 cgtaagaagc aagagcgttt ttcagaagtg tttacagatt ttttatttga aaaatctttc
     1381 tcagtgatct ttgtgtgctc cgtggcgaag taatatgttt agaaagttaa taattggaaa
     1441 agtattatag ctagaattaa ccccagttca aactgaggct gtgggtggac tgtctcgtca
     1501 gtcattccta tggtcttctt ttgagttgct gcataatggc ctgccgctga gatgcgggtg
     1561 agcaagccaa cgccaacaaa acttacgttc ctttgaccta cgctggcact gagcgcgata
     1621 cccaggcagg gctggcgagc gcccccccgg tagctccagc ggtgtgttag gcagcatgtc
     1681 aggctcccta gcctgtcagg tctctgcatc tgtgaaggaa tggagttccc tgatgcggtc
     1741 ccggactcgc tcgataacgc tgacagctgt cctagggaat aaattgtcgt tcgggtcgtt
     1801 gacccaggtc atgaactctt tgagaccagg atggagtact actttgagcc gctgttcgat
     1861 ctcagtagct gtaagggccc ttctagcgtc ctcgtcccaa acgtggccga cgaccttgtt
     1921 gcgaaaatca aggatccctt tcgcttcaat caccttacta agcgtcttag ccacatcgcg
     1981 aacgtcctcc gggatgacgt ctctatagcg gtcgtagaac tccttccact tcgagagtgt
     2041 gatgatgaga tggaataggc aaagacgatt gacttgg
//
LOCUS       DATDRL010000058         5760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_171678, whole genome shotgun sequence.
ACCESSION   DATDRL010000058 DATDRL010000000
VERSION     DATDRL010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171678"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VI935_02005"
     CDS             complement(<1..623)
                     /locus_tag="VI935_02005"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HLE24405.1"
                     /translation="MLLSLVKGHGLILNAIFSVVLLYSISTHFLEAKEKNSGLSSINS
                     GDIVGGVKSSPSYQSYEVIVKTNVFKGVKSAQSNKDRLSVDLSTTRLNLTLLGTVIGK
                     GSSTAIIKNPSTGKISSYQQWDSIDLINTEQVKLVEISKCMVMIERDDRYETISCNNG
                     YVSEAAKHALITPLARYKIITTSGKQYKELYISKSKYEDEIQISSEK"
     gene            complement(686..1267)
                     /locus_tag="VI935_02010"
     CDS             complement(686..1267)
                     /locus_tag="VI935_02010"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HLE24406.1"
                     /translation="MYFEPYTEYRSHVSKISLRTADGKEVYIEIGPGASFGAEHMTTR
                     LCLRGIEEIFKINKIKTVLDLGCGSGILGIAAKALGAENVFLIDIDPLAVEEAKGNAL
                     RNGVDSSVLIFYGSLENIKRKFELVIANIVTGELLRMGEGIKSLFDNEGFLLVSGISE
                     LKKDEALSGLIDIGFSFNKEFIEGGWVAMLLNL"
     gene            1950..2564
                     /gene="tmk"
                     /locus_tag="VI935_02015"
     CDS             1950..2564
                     /gene="tmk"
                     /locus_tag="VI935_02015"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HLE24407.1"
                     /translation="MFITLEGVEGSGKSTQAELLKEFLEKQGRKVTLTREPGWGALGR
                     LIRRLLLEEKDLELDPLAELYLFCADRTQHVRDFILPKLKEGGVVICDRFTDSTIAYQ
                     GYGRRLELDLVERVAKEAALGLKPKLTILLNIPVKVGLSRLEKREGKTKMDEEPLDFH
                     ERVRQGYIFIAQKEPKRVKVVSATRDVYEIQQEIRNIVLSHISK"
     gene            2572..3591
                     /locus_tag="VI935_02020"
     CDS             2572..3591
                     /locus_tag="VI935_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HLE24408.1"
                     /translation="MFFTSIIGHEFQKQALRRAALENRVSHAYLFFGPDGVGKKLVAI
                     ELARVLNCIHLNDRIAGEKLETNTCDCISCKKIKKGIHPDVFLIEYGGIKDIKVDQIR
                     EGVEDRLYFKPFEGRFKVAIVDEAHRMNLNAQNAFLKTLEEPPSDSVIILITSQPQVL
                     LPTIRSRCQSFEFKPLSQDVIVEEIIKRKKLPRDEAILVARLSGRSLGKALSLDEDLL
                     SERKEIIRKLSSINPNSATQVLNFVESLPRGSTSQDVNKLMFLFDIILLWLRDVVLIK
                     IGFSPDSLSNRDLIHLTRKLADSWSMDNVLDKLKFLERAWYDAFRGNVNKQIMLENLV
                     LKITE"
     gene            3755..5371
                     /gene="metG"
                     /locus_tag="VI935_02025"
     CDS             3755..5371
                     /gene="metG"
                     /locus_tag="VI935_02025"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HLE24409.1"
                     /translation="MPKTFYVTTPIYYVNDAPHIGHAYTTIAADVIARFRRLMGDKVF
                     FLTGTDEHGQKIERVALASGERPIELADRVVLRFKGLWDKLNIMNDDFIRTTEERHIK
                     AVLELFQRVFDRGDIYLGEYEDWYCVFDETFLTETQLVVRSDSLQGERACPDCGRPVE
                     KLKEESYFFRLSKYQDPLLEFYEDNPDFVQPSFRRNEVINFVKGGLRDLSISRTSFSW
                     GIPIYFHSTQTADEMLDNLRDKNPSEAKQHVIYVWFDALTNYLTAAGFPDDSERFNEI
                     WPADIHLVGKDILRFHTVYWPAFLISAGLTPPHKVFAHGWWTVEGEKMSKSKGNVVDP
                     YAIIDEFGADVLRFFLLREVPFGLDGDFSRNALIGRINGDLANGLGNLVSRSLGMIEK
                     YSGGKVPKPGSFEPFDLSVREKALATARQVEEAINEIAFHKALGAIWDFIATVNKYVD
                     ESAPWALEKAGKRERLDTVLWTLVESIRVITLLVYPFIPKSAEEIWERIGIPQPIESQ
                     PLSNAKNWGLLKPGLKILKGESLFPRYGVK"
     gene            5372..>5760
                     /locus_tag="VI935_02030"
     CDS             5372..>5760
                     /locus_tag="VI935_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24410.1"
                     /translation="MLYNPEKHRRRTIRLKEYDYSQAGAYFITICTKNRERIFGDVVG
                     ETMNLSVVGKIAKMFFEAIPSHFQSVALDEFIVMPNHLHGIIIIQNVGVQNFEPLQKQ
                     NIFQHIIPKSLGSIIRTYKSAVTHWCR"
BASE COUNT         1773 a         1054 c         1254 g         1679 t
ORIGIN      
        1 tatttttcac tggaaatttg aatttcatct tcgtacttag atttagatat atataattct
       61 ttgtattgtt tgccagatgt agtgattatc ttatatcttg caagaggagt aataagagca
      121 tgttttgcag cttcactcac atacccatta ttacaactga ttgtctcgta tctatcgtct
      181 ctttcaatca ttaccataca ttttgaaatc tcaactagct taacctgttc tgtattaatc
      241 agatcaattg aatcccactg ttggtaggaa ctaatcttac ctgtagaagg gttttttata
      301 atagctgttg aggatccttt tccaataact gttccgagta gagttaaatt tagccttgtt
      361 gttgaaagat caaccgagag gcgatcttta ttagattgag cagatttcac tcctttaaaa
      421 acgttcgttt ttacaatgac ttcatatgac tgatatgaag gcgatgattt tacaccacct
      481 acaatatcac cgctatttat tgaggataaa ccagaattct tttctttagc ttctaaaaag
      541 tgagtactaa tggaatatag gagcacgact gaaaagatag cgtttaaaat caagccatgc
      601 cctttaacga gtgaaagtaa catttttagc ccaaatagaa taattaaact tgaggcaaaa
      661 gtaaataagt agagttgaat ttaccctata agtttaacaa catagcaacc caaccacctt
      721 ctataaattc cttattgaaa ctaaacccaa tgtcgataag ccccgataat gcctcgtctt
      781 tttttaactc ggagatcccc gaaaccaaga gaaaaccttc attatcaaat agagatttaa
      841 taccttcacc cattcggagg agttcacccg ttactatatt tgcaataact agttcaaatt
      901 ttctttttat attttctaag gaaccataaa aaattaaaac gcttgaatca actccatttc
      961 ttagagcatt tccctttgct tcctctactg ctaagggatc aatgtcaata agaaaaacat
     1021 tttcggctcc gagtgcttta gccgcaattc caagaatgcc actaccacac ccaaggtcca
     1081 aaacggtttt tattttattg attttgaata tttcctcaat tccccttaag caaagccttg
     1141 tcgtcatatg ctctgcacca aaggaagctc caggccctat ttcgatatat acctccttcc
     1201 catctgcagt ccttagacta atcttagaga catgtgaacg atattcagtg tatggttcga
     1261 aatacacttt gctagggttc tcaataatac tcaaatacta tttttagtaa ggaataatat
     1321 aaaatttaac ctttgatgcc gcaaaaatca aaaaagggat gagaaaaaat acatgcagtt
     1381 gatttaggtt ggacgtttgt tatatactga ctaacagtga cgggtgttac aaggtacgtg
     1441 ggctaatttc atatctaatg tactaaggat ttccgcataa tcactagtta ggcagtcggg
     1501 ataagagatg gccgtttaca ggctgggaat gttcaagtga gtaaccaaga cccaagtggc
     1561 ggttggcacg acgcctggag ggcacccctc gccactgtca ttggtggagg acagtatcaa
     1621 tggggtcgcg tctcaacttt tgacattcat tctctcgtta tgtcaaatat tgagacatga
     1681 ccccttcgtt acacatctct tgaccgcagt gccatcttta acgagaattg tagtcggagc
     1741 tttaaataaa gactttaaaa agaggccaat gactaaaaga atataacaaa ttatctttac
     1801 tattatttct tacttgtgat agaattttct ttgtcttcaa tgacatgatg tttttttgag
     1861 agcatagaag ctttatcgtc aatattgaat tctaagggtg attttctata ttgaacggag
     1921 catgtggtaa accgggagca tatagagata tgtttattac acttgaaggc gtagagggaa
     1981 gtgggaaatc aactcaggcg gagcttctta aggagtttct tgagaaacaa ggccgtaagg
     2041 taacccttac aagggagcct gggtggggcg ctcttggaag actcatccgc aggttactcc
     2101 ttgaggaaaa ggatctagag ttagaccctt tggctgagct ttatcttttt tgcgcggatc
     2161 gaactcagca tgtaagggat ttcatattac caaagctaaa ggagggtggg gttgtgatat
     2221 gcgacaggtt tacagattca acaattgcct atcagggcta cggtagaagg cttgagttag
     2281 acctagttga aagggtagca aaagaggcag cccttggttt gaagcctaag cttactatac
     2341 ttctcaacat tcctgtaaag gttggcctct cacgacttga aaaaagagag gggaagacta
     2401 agatggacga agagccttta gacttccatg agcgggtaag gcagggatac atttttatag
     2461 ctcaaaagga accgaaaagg gtaaaggtcg taagtgcgac aagggatgtt tatgagatac
     2521 aacaagaaat aaggaacata gtgctttcac atatatctaa gtgattttcc aatgtttttc
     2581 acctctataa taggtcatga atttcaaaaa caagcattaa ggcgagcggc tctagaaaac
     2641 cgagtctctc acgcatatct cttttttggt cctgatgggg tagggaaaaa acttgtggca
     2701 atcgagcttg caagggttct taattgcatc cacctgaatg atcggattgc aggggaaaaa
     2761 ctagaaacca atacatgtga ctgcatttca tgcaagaaga taaaaaaggg aatacatcca
     2821 gatgtgtttc ttatagagta cggagggata aaggatatta aggttgatca gatacgtgag
     2881 ggggtggagg ataggttata ttttaagccc tttgaaggaa ggttcaaggt agctatagtt
     2941 gacgaggctc atagaatgaa cctaaacgca cagaacgcat ttctcaaaac cctggaggag
     3001 ccaccgtctg actcagtaat cattcttatt acatcccaac ctcaggttct tctcccaaca
     3061 atccgttcaa gatgccagtc ttttgaattt aagccgttat ctcaggatgt aattgttgaa
     3121 gagattataa aaagaaaaaa attgcctcgc gatgaagcaa tccttgtagc aaggctatca
     3181 ggtagaagtt tgggaaaggc gctcagtcta gacgaggacc ttctttctga gagaaaggaa
     3241 ataatcagga aactatcaag cataaaccct aacagtgcta ctcaggtact taactttgtg
     3301 gaatctttgc caagaggttc aacatctcag gatgttaata aacttatgtt tctcttcgat
     3361 attattttac tctggcttcg tgatgtggtg cttatcaaga ttgggttttc tcccgattct
     3421 ctttcgaata gggatctaat ccacttaaca cgtaagctag cggacagttg gagtatggat
     3481 aatgttttgg ataaactcaa gtttcttgaa agagcttggt atgatgcatt tcgcggaaat
     3541 gtaaataagc agattatgct tgagaatttg gttttgaaga ttacagagta ggtgattcgt
     3601 tgcaaggtgc ccctaagttt tgtcattgcg agcaactgca aggagcgaag caatctaaaa
     3661 aacaatcgag ggattgcttc acttcattcg caatgacaga agggggggtt attactcaat
     3721 gtggaaaaac ataatggatt ggagataaaa tctgatgcca aagaccttct atgtaacaac
     3781 cccgatttac tatgttaatg acgctccaca cataggacac gcttacacca caattgctgc
     3841 agatgtgatt gcaagattca gaaggctcat gggggataag gtgtttttcc tgacgggcac
     3901 agatgagcat gggcaaaaga tagaaagggt agcattggca tcgggcgaaa ggccaattga
     3961 actagcggat cgagtagtcc ttaggttcaa gggtctttgg gataaattaa atatcatgaa
     4021 cgatgatttc ataagaacaa ccgaagaaag gcacattaaa gcagtactgg agcttttcca
     4081 aagggtcttt gatcgtggcg acatctatct tggagaatat gaagactggt attgtgtttt
     4141 tgatgaaaca ttcttgaccg aaacccagct tgtagttcgg tctgactcac tccagggaga
     4201 aagggcgtgt cctgattgtg gtcgccccgt tgagaagctg aaagaggaga gttatttctt
     4261 taggctttca aaataccaag acccactttt ggagttttat gaagataacc ctgattttgt
     4321 tcagccatcg tttagaagaa acgaggtcat aaactttgta aagggcgggc ttagagacct
     4381 cagtattagc aggacatctt tttcatgggg gattcctatt tatttccatt ccacccaaac
     4441 tgcggatgaa atgctcgata atctaaggga taaaaacccg agtgaagcga agcaacacgt
     4501 aatatacgtc tggtttgacg cgctcacgaa ctatctcact gcagccggtt ttcccgatga
     4561 ttcagaaaga ttcaatgaaa tctggcctgc ggatattcac ctagttggaa aggacatact
     4621 gagattccat actgtatact ggccagcgtt tttaatttca gcagggctta cgcccccaca
     4681 taaggttttt gcacacggct ggtggaccgt agaaggggaa aagatgtcta agtcaaaggg
     4741 gaatgttgta gatccttatg caattataga tgaatttggt gctgatgttt taaggttctt
     4801 ccttcttagg gaggttccgt ttggactaga tggtgatttt tcaagaaatg cccttatcgg
     4861 aaggataaac ggcgaccttg caaacgggct tgggaatctt gtaagccggt ctttagggat
     4921 gatagaaaag tattcagggg gcaaggttcc taaaccaggc tcattcgagc catttgacct
     4981 tagtgtaagg gaaaaggcgc ttgctactgc aaggcaagta gaagaagcga ttaatgaaat
     5041 tgcttttcac aaggctcttg gggctatctg ggattttatt gctactgtaa acaaatacgt
     5101 ggatgaatcc gctccatggg ctcttgagaa ggctgggaaa agagaaagac ttgatacagt
     5161 actctggacg cttgttgaat caatacgagt aatcaccctt ttggtatacc cttttattcc
     5221 taaatcggct gaagaaattt gggaaaggat aggcataccc cagcccattg aatctcagcc
     5281 tttatcaaat gcaaagaatt ggggactact caaacctggt ttgaagattc taaagggtga
     5341 gagtttattc ccgaggtatg gggtgaaata aatgttatat aatcctgaaa agcatcgccg
     5401 acgcaccatt cgattgaaag aatacgatta ttcacaggca ggtgcgtatt ttataacaat
     5461 atgcacaaaa aacagggaac gcatttttgg ggatgtggta ggtgaaacca tgaatttatc
     5521 tgtagttgga aagatagcaa agatgttctt tgaagcgata ccaagccatt ttcaatctgt
     5581 agcattggat gaatttattg tgatgccgaa ccatttacat gggataatta ttatccaaaa
     5641 tgtaggggtt caaaattttg aacccctaca aaaacaaaat atttttcagc atatcatacc
     5701 taaatcatta gggtctatta tccgcacata caaatctgcg gtaacccatt ggtgtagaaa
//
LOCUS       DATDRL010000059         7771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_438227, whole genome shotgun sequence.
ACCESSION   DATDRL010000059 DATDRL010000000
VERSION     DATDRL010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7771
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438227"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /gene="mtnA"
                     /locus_tag="VI935_02035"
     CDS             <1..784
                     /gene="mtnA"
                     /locus_tag="VI935_02035"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HLE24411.1"
                     /translation="ISNTRPTAVNLFWAANRMMNLVNENKDKDIDWIRDRLVEEAKKI
                     YEEDIQICKRIGRVGSELIKDRATILTHCNAGALATAGYGTALGVVRAAFEEGKGVKV
                     IASETRPFLQGARLTAWELDKDGIPVTIITDSMAGYVMSNGMVDIVVVGADRIASNGD
                     VANKIGTYSLSILARFHKIPFYVAAPISTIDFNCSSGDLIPIEKRDIKEVTHILGNKI
                     TPDVEVLNPAFDITPSDHISSIITDRGIAEAPYSKSLRSLLS"
     gene            complement(781..1359)
                     /locus_tag="VI935_02040"
     CDS             complement(781..1359)
                     /locus_tag="VI935_02040"
                     /inference="COORDINATES: protein motif:HMM:NF044994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HLE24412.1"
                     /translation="MKGKKKKEKKNKKKVLRGKESYKKKTKIPKRKISLKKTKKVRGK
                     KKQGGILLKPETKKTGTRGRTLKSPRKEEKPKWMNGIKDMLIEMRKELLKDVSQSAKM
                     ERDYLRFDIGDFYDHASSDRDRELMLTLTDRERDKLVLIDEALKRIKDGNYGICENCG
                     DEIGEDRLRAMPFAKFCLSCKIDLERQESRNI"
     gene            1532..2500
                     /gene="gshB"
                     /locus_tag="VI935_02045"
     CDS             1532..2500
                     /gene="gshB"
                     /locus_tag="VI935_02045"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF003573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HLE24413.1"
                     /translation="MKLRMAFVMDAIETTNIERDTTFVLMLESQNRGHEVWYLELKDL
                     FSENGKAWGTVSEINLKIASDFFKILGRATVPLEHFDVVWMRKDPPFNIDYIYATYIL
                     SLINPEKTLVVNNTKGLKESNEKFYALSFPELVPPTVVAKDMNKLKGFLTGIGGEMVV
                     KPLDGCGGEGVFYIKEGDRNANVILESITDGGKRFVLAQKFIKEVSQGDKRIIILNGT
                     PLGAVLRIARPGGEFRCNFHSGGSPAKTELTERDIEICQRIAPKLREDGLYFVGIDVI
                     GGYLTEVNMTSPTGVQEINRLCGTKLESVVIDFVEEVCNKNYLATN"
     gene            2595..2885
                     /gene="gatC"
                     /locus_tag="VI935_02050"
     CDS             2595..2885
                     /gene="gatC"
                     /locus_tag="VI935_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HLE24414.1"
                     /translation="MKISKDAVRHVAELARLEFKEDELEKFSEQLGNILEYMEKLNEI
                     NTASVEPTFHVLGLSTSLREDVVKPWLSLDEALENAPEKEEGFFTVPKFIED"
     gene            3051..4511
                     /gene="gatA"
                     /locus_tag="VI935_02055"
     CDS             3051..4511
                     /gene="gatA"
                     /locus_tag="VI935_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HLE24415.1"
                     /translation="MELPFLSIREVSELINRKEVSPVELTHSILDRIEDVDSKIHAYI
                     TILRGEALKAANEAEKEISSGTYLGALHGVPISVKDIFVMKGIRTTCGSKILENFVSP
                     YDAMVVEKLKHAGAIIVGKNNMDEFAMGSSTENSYFGPTRNPWDLERVPGGSSGGSAA
                     ATSASICFASVGTDTGGSIRQPAALCGIVGLKPTYGRVSRFGMIAFASSLDQGGPLTK
                     TVKDAALMLNVIAGGDARDSTSVHIPVPDYARQLDGEIKGIRIGVPKEYFLKGIDVEV
                     EEAVKNAIRLIEDLGCPVEEISLPHTKYAVSTYYLIAPSEASSNLARYDGVRYGFRFP
                     DSSSLREMYHRTRAEGFGAEVKRRIMIGTYALSAGYYDAYYLKAQRVRTLIKRDFEEA
                     FKKVDIIITPTSSEAAFKIGEKTDDPLKMYLSDIFTIPCNLAGLPGISIPCGFTSGGL
                     PIGLQILGKPFDEETLLRVAHAYELRTNWRERKPPI"
     gene            4666..4839
                     /locus_tag="VI935_02060"
     CDS             4666..4839
                     /locus_tag="VI935_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24416.1"
                     /translation="MSEKRRVCMVCGKPSEVSICDACKAQIQGEAIDKKQKIEREVRI
                     GPEIKKEKEKKKK"
     gene            complement(4902..5744)
                     /gene="rsmI"
                     /locus_tag="VI935_02065"
     CDS             complement(4902..5744)
                     /gene="rsmI"
                     /locus_tag="VI935_02065"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HLE24417.1"
                     /translation="MQGKLYIVSTPIGNLEDITLRAIRVLKEVDLIACEDTRVTKKLL
                     SHYQIQKPLTTYHEHNEKEKAVELLSLLQAGKNIAVVSNAGTPGVSDPGYRIVSLASQ
                     KCIEITSIPGPCAAIGALSVSGHPTSSFSFFGFLPKPAKKRKDFLELIRESSQTLIFY
                     ESPNRVIETLEDMLKILGDRKISVSRELTKMFEETLRGKTSEVLETLSQRQKLKGEFT
                     ITVEGYHNEIDNIEELLEEQLNIYKEKGLSLRDAVKEVSRKLMFSKSKTYKVALKLWE
                     NREK"
     gene            5953..6945
                     /locus_tag="VI935_02070"
     CDS             5953..6945
                     /locus_tag="VI935_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24418.1"
                     /translation="MIEQVHKVIYWLHRYSDISKLLLRELEGVLIRIEGYEFSRKGNL
                     LNNFGVTKNESNFTSAYSELFLTNFFIKNGIKLIEYEPLTKDGIYKADFKICFDSDNS
                     VTVELVTPGQEITDFETKADFLFEKLERVRSGLSIEVSGFESYDSSDLWRTKVEPPTH
                     KNIQEIISNFRSYVPSLSDHELPKELPTLCQDYPRIKVIVDHRIPNYGGTFVALSSSR
                     TAEGFPAGRIIKLILDEREHLSPDDCNLVFVDFSYWSQIDRHYLDTEVYRKVLIEELE
                     RKMSSRIDGVLTYMLSNQKDERLINRRILWLNSSKHWVNAKEFQCFLKFWESWN"
     gene            complement(7487..>7771)
                     /locus_tag="VI935_02075"
     CDS             complement(7487..>7771)
                     /locus_tag="VI935_02075"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019503336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HLE24419.1"
                     /translation="EIQKEFGSFDKYIWRFVGGKPIKHTVKSLSELPSTTKESDAMSK
                     DLVKRGFKFVGSTICYAFMQAVGMVNDHTINCFRYDEVSKIKSTPRQRKT"
BASE COUNT         2349 a         1326 c         1638 g         2458 t
ORIGIN      
        1 aatttcaaac acaaggccta ctgcggttaa ccttttctgg gcggctaaca gaatgatgaa
       61 tttggtgaat gaaaataagg ataaggatat tgattggatt agagataggt tggttgaaga
      121 ggcgaagaag atctatgaag aggatattca aatttgcaaa agaatcggaa gggtaggttc
      181 tgagttaata aaggacagag ctacaatcct tacacactgc aacgctggcg ccctagccac
      241 agcgggctac ggcactgcgc tgggagtcgt tagagcggct tttgaagaag ggaaaggggt
      301 taaggtcatc gcatcggaaa caagaccctt tttgcaaggt gccaggctta ctgcttggga
      361 gttggataaa gatggcatac cagtcacaat aattaccgac agtatggctg gttacgtaat
      421 gagtaacggc atggtggata ttgtagtcgt tggtgcagac aggattgcgt caaacgggga
      481 tgttgcaaat aaaattggaa cctattctct atctatactg gctagatttc ataaaattcc
      541 gttttacgtt gcagccccca tttctacgat tgatttcaat tgttcttcag gggatttaat
      601 accaattgaa aagcgtgaca ttaaagaggt aacacatata ctcgggaata aaattacacc
      661 ggatgtggag gttttaaacc ctgcatttga tataacgccg agcgatcata ttagttctat
      721 aataactgat agaggaatcg ccgaagcgcc ttattctaag agtttaaggt cgcttcttag
      781 ttagatattt ctactttctt gtctttctag gtcaatcttg caagagaggc aaaactttgc
      841 aaaaggcata gcccttagtc tgtcttcgcc tatttcatca ccacagtttt cgcaaattcc
      901 ataatttcca tctttaatcc tttttaaagc ctcatctatg agcactagct tatctctttc
      961 tctatcagtg agggtaagca taagctccct gtctctatcg cttgaagcgt gatcatagaa
     1021 gtcgccgatg tcaaatctta gataatctcg ttccatctta gcggattgtg agacatcctt
     1081 taggagttct tttctcatct cgatgagcat atcttttatt ccattcatcc atttaggttt
     1141 ttcctcttta cggggtgatt ttaaagttct tccccttgtc cccgtttttt ttgtttctgg
     1201 tttcaaaaga atgcctcctt gttttttctt accccttact ttttttgttt tttttagaga
     1261 gatctttctt ttcggtattt tagtcttttt cttgtaactc tctttgcctc ttaagacctt
     1321 tttcttgttc ttcttttctt tctttttttt acctttcatt gctcccccaa ttttatcctc
     1381 tttttacaag gaattattag aataagcata taaagcaatt tgtcaagagt gatctagcgc
     1441 gcgtgtctag ctcgcatttc tatcataact cctttttcaa tatcacatgt caattcttga
     1501 ctccatagtg gttttagata gattttacgc tatgaaactt agaatggcgt tcgttatgga
     1561 tgcgattgag acaactaata ttgagaggga tacaacattc gtactaatgt tagaatcgca
     1621 gaatagggga catgaggttt ggtatcttga gcttaaggat ttatttagtg aaaacgggaa
     1681 ggcttggggt acggtttcag agattaattt aaaaattgcg tcggattttt ttaaaatttt
     1741 aggaagagct acggtaccac ttgagcattt tgatgtagtc tggatgagaa aggatcctcc
     1801 ttttaatatt gattatatct atgccaccta tattcttagc ttgatcaacc cagagaaaac
     1861 actggttgtg aataatacta aagggctaaa agaatcgaat gaaaagttct acgctctaag
     1921 ctttcctgag ctagttccac caacggttgt tgcgaaagac atgaacaagc taaaaggttt
     1981 tcttacaggg atagggggtg agatggttgt aaaaccactt gatggatgcg ggggagaagg
     2041 tgtgttttat attaaagaag gtgatagaaa tgcgaatgta atattagaaa gcattacgga
     2101 tggcgggaag cgtttcgtac ttgcgcaaaa atttatcaaa gaggtatctc aaggggataa
     2161 aagaattatt attcttaacg gaacaccgct tggtgcagtg ctacgtatag cgagacccgg
     2221 aggagaattt cgatgcaatt ttcactcagg cggaagccct gctaaaacgg agctaactga
     2281 gagggacatt gaaatatgtc agaggatagc accaaagctt agagaggatg gactttattt
     2341 cgtaggaata gatgttatag gtggttatct tacggaagta aatatgacaa gccctacagg
     2401 tgttcaggag ataaatagac tttgtgggac taagttggag tctgtggtta tagattttgt
     2461 tgaagaagta tgcaataaaa attatttagc cacaaattaa cacggatgaa cacgaatgaa
     2521 atgaaaattt aatgtttttg catttattcg tgtaaattcg tgtccattca tggctaatta
     2581 agaaggagca gcacatgaag atttcaaaag atgcagtgag acacgtagca gagcttgcca
     2641 ggctagagtt taaagaagat gaacttgaga aattctctga gcagttaggg aacatccttg
     2701 agtacatgga aaaactaaat gagattaata ccgccagtgt tgagcctacg ttccatgtcc
     2761 ttgggctttc gacttctcta agagaagatg ttgtaaagcc atggctaagc ctggatgaag
     2821 ccttagaaaa cgctccagaa aaggaagaag gtttttttac agtcccaaaa tttatagagg
     2881 attagaggca tttagccact aaggcacaaa ggagaaatat gagtttttaa aaggtatata
     2941 caggagtcag aaaccagaac ccagaagact tctgaattcc gacttctggc ttctaatttc
     3001 atgttttagt gtctttgtgg cttgactatt acaagcctta aggggataag atggaactac
     3061 catttctttc aatacgtgag gtttcggagt taataaatag aaaagaggtt tctccagttg
     3121 aacttactca ttcaatcttg gataggatcg aggatgttga tagcaagata cacgcttata
     3181 taaccatctt aagaggggag gcactcaagg ctgcaaatga agcggaaaag gagatttctt
     3241 caggaaccta tctcggtgcc cttcatggtg ttccgattag tgtaaaggac atctttgtta
     3301 tgaagggaat acgaactact tgcggatcca agatattaga gaattttgtt tctccctacg
     3361 atgctatggt agttgagaag ctaaaacacg ctggcgctat tattgtcggg aaaaataaca
     3421 tggatgagtt tgcaatgggt tcatctacag aaaattccta ttttggacca acaagaaacc
     3481 cgtgggattt agaaagggtt ccaggtggct caagcggtgg ttcggccgcc gctacatcgg
     3541 catcaatttg ttttgcatcc gttggaacag acacaggggg ttctataagg cagcctgctg
     3601 cactatgtgg gattgtcggg ctaaaaccta cttatgggag ggtaagcagg tttggaatga
     3661 tagcctttgc gtcatcattg gatcaaggag gacctctcac aaaaactgtt aaagatgcgg
     3721 ctttgatgct aaacgttata gcaggcggag acgcaagaga ttcaacctct gtacatattc
     3781 ctgttcctga ttatgcaagg caacttgacg gtgaaattaa ggggattaga atcggggttc
     3841 cgaaagaata tttcctaaag ggaatagatg tcgaggttga ggaagcagta aagaatgcta
     3901 ttagactcat tgaagacttg ggttgtcctg tagaagaaat ctctcttccc cacacaaagt
     3961 atgctgtttc cacgtactat ctcattgccc catctgaggc tagttcaaac cttgcaaggt
     4021 atgatggagt aaggtatgga tttaggtttc ctgactcaag ttcacttagg gagatgtatc
     4081 atagaacaag agcagagggt tttggcgctg aggtcaaacg gcgaattatg attggaacgt
     4141 acgcgctttc tgcgggatat tatgacgcct actatcttaa agctcaacgt gtgagaaccc
     4201 ttataaaaag ggattttgaa gaggcattca aaaaggttga tataattata actcctactt
     4261 catctgaggc tgcatttaaa attggagaaa agactgacga tcctttaaag atgtatctat
     4321 cggatatatt cacaattcct tgcaaccttg ctgggcttcc aggcatttct attccctgtg
     4381 gatttacatc aggaggtctt ccgatagggc ttcagatact cggaaagcca tttgatgagg
     4441 agacactgct ccgagtagcc catgcttatg aactgcgcac aaattggagg gaacgaaaac
     4501 cgccgattta aaatagagga cggaagccag gagtcgggag acggaaatca gcagattcta
     4561 acttctgaat tctgacttct gacttctaaa ttctgtcatc tctgtagtta catatttttg
     4621 atttggtatt tgatgcttgg aatttgtttt aattaaggag gaaatatgtc agaaaaaagg
     4681 agagtttgca tggtatgtgg aaagccttct gaggtttcaa tctgtgatgc ctgtaaggct
     4741 cagattcaag gggaggcaat agataaaaag cagaagatag aaagagaagt gagaattggt
     4801 cccgagataa aaaaagaaaa agagaagaaa aagaaatagt agggactacc cttgtaattg
     4861 ctatttgaat ttttccttct ggatgaacat gaggttcgcc cttacttctc acggttttcc
     4921 cagagcttaa gtgctacctt ataagtttta cttttagaaa acattaattt tctagaaacc
     4981 tctttaaccg catctctcaa gcttaaccct ttttccttat aaatattaag ttgttcttca
     5041 agcaattctt ctatgttgtc aatttcgtta tgatacccct cgacagtaat cgtaaactct
     5101 ccctttaact tttgtctttg actcagggtt tcgaggacct cagaagtctt tcccctgaga
     5161 gtttcttcaa acatcttagt aagctctcgg ctcacagaga tttttctatc tccaagaatc
     5221 ttaagcatat cttctaaagt ttctattacc ctattaggag attcatagaa tattaaggtt
     5281 tgagaagatt ctcgtatcaa ttctaaaaaa tcctttcttt tttttgccgg cttgggaagg
     5341 aatccaaaaa aggaaaaact tgaagtagga tgccctgata cacttagtgc tccaattgca
     5401 gcacaggggc ctggaataga agttatttca atgcactttt gtgaggcaag gctaactatt
     5461 ctatatccgg ggtcagaaac tccgggtgtt ccagcattcg agacaactgc tatatttttc
     5521 ccagcttgta gaagagacag aagctctact gctttttcct tttcattatg ctcatggtat
     5581 gtagtgagag gtttttgaat ttgataatgg gaaaggagtt ttttcgtaac cctagtatct
     5641 tcacaggcaa taagatcaac ctcttttaga accctaattg ctctaagcgt aatatcctca
     5701 agatttccta taggtgttga aacgatgtag agcttgcctt gcattggttt gggagtatag
     5761 attactcaaa gtatgcagtt tagtaaaaag atatatgcta cgatatgtta tgatcttaat
     5821 atgtctaata aattaaaagc cgattttaca attaacattg agaatgtctt aaaaagggct
     5881 aagagctacc ctcgaatatc caaagtaatc caaccccgat tagaagaact atctaagttt
     5941 aaacaacctc cattgataga acaggttcat aaagttatat actggcttca cagatattca
     6001 gatattagta agcttttatt aagggaatta gaaggagttc ttattagaat agagggttat
     6061 gaatttagta gaaaaggaaa tttactaaat aattttggtg taactaaaaa cgaatcgaat
     6121 ttcaccagtg cttattctga attgttccta actaacttct ttattaaaaa cgggattaaa
     6181 ttgattgaat atgaaccctt aacaaaagat ggaatataca aagctgactt taagatttgt
     6241 ttcgattctg ataattctgt gaccgtggaa ttggtgactc ctggacagga aattacggac
     6301 tttgaaacca aagctgattt tctatttgaa aagttagagc gtgtaaggtc tggattatca
     6361 attgaggtta gtggctttga gtcttatgac agttcagacc tatggcgaac taaagttgaa
     6421 cctccaaccc acaagaatat acaagaaatc atcagtaatt ttagaagcta tgttcctagt
     6481 ttgtctgatc atgaactccc taaggagcta cccactctat gtcaggatta tccaaggatt
     6541 aaggtaatag ttgatcatag aataccaaat tatggaggca cctttgttgc tttaagctct
     6601 tcgaggactg ctgagggttt tcctgcaggt agaattataa agttaatttt agatgaaagg
     6661 gaacatttat ctccagatga ttgtaacttg gtttttgtgg attttagtta ttggagtcaa
     6721 attgatagac attaccttga tacggaagtt tataggaaag ttcttataga agaattggag
     6781 agaaaaatgt cttcaaggat tgacggggtt ttgacatata tgttaagtaa tcagaaagat
     6841 gagaggctta ttaatcgcag aatcttatgg ttgaattcaa gcaaacactg ggtaaatgca
     6901 aaagaatttc aatgtttttt gaagttttgg gaatcgtgga actaactttg atgtttttct
     6961 attcaattac ttctttctta gaattttgag attttttaat ctcactacat agcatcgtct
     7021 gggtctattc tacttttact atttcgcaca gcaatatgct atcttcacgc aatctgataa
     7081 agtttcccgg ctctaataca gcgataaaat cattgacttc atcttcgtat agcactgtta
     7141 gggtgtgttt gaaaacttta ttacattcga tttatccatt gagtctgtaa aaccttacga
     7201 attccttttc tttcatattt ttgttccaat taagaatact aagtattatt taatagtgtt
     7261 ctttagtgca aagaacaaat tccaagcacc aaatcacaaa tttcaaacaa ttttaaattt
     7321 ctaaattcta agcattcgga attgaaaatt cggatttgtt tgtaatttgg aacttgatca
     7381 tcggtgcatg ttcttcgatt aagcaaatga cgttagaatg ccgatgtctt tttgaaaata
     7441 tctgaactga atttgcgtgc tgtgaagtac agcggaaaga ataatttcaa gtttttcttt
     7501 ggcggggagt actttttatc ttagatactt catcatatct gaaacaatta atcgtgtggt
     7561 catttaccat accaactgct tgcataaagg cgtagcaaat agttgaacca acgaatttaa
     7621 agcctctttt aactaaatct ttactcattg cgtcggattc tttagttgtc gaaggtaatt
     7681 ctgaaagcga cttaaccgta tgtttaattg gtttaccacc aacgaaccgc caaatatatt
     7741 tatcaaaaga accaaactct ttttgaattt c
//
LOCUS       DATDRL010000060        16765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1226158, whole genome shotgun sequence.
ACCESSION   DATDRL010000060 DATDRL010000000
VERSION     DATDRL010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 16765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16765
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226158"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VI935_02080"
     CDS             <1..500
                     /locus_tag="VI935_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24420.1"
                     /translation="SSPVSGTVLEVRRFEISPSFSGREFVYRIGDLSYESDFYNQFFR
                     PPNLLTTDEVRKWFSESGLFKNVVVPSNNVESDYVLEGNVSELYGDFRATNEPKAVLG
                     IQFFLTEETSTNPKIVFENNYRREVVLRSNSAQDLVKGWNEALQQILTALEGHLKNVD
                     FKTPK"
     gene            832..1446
                     /locus_tag="VI935_02085"
     CDS             832..1446
                     /locus_tag="VI935_02085"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HLE24421.1"
                     /translation="MVRIKICGITNIEDALLSVDLGANALGFVFYQGSKRYIKPDNAQ
                     TIISKLPPFVTTVGVFVNQILEDIIKIKEKAGFDAFQLHGEESPDFCMKLKGKVIKAI
                     RVKDSIDPKEIESYPTQAILFDNYSMEYYGGAGETFGWEVLRGLDTSKKIILSGGLTS
                     ENVANAIRIVNPYAVDVSTGVEEIPGKKDPGKLKRFIEAARNET"
     gene            1436..2638
                     /gene="trpB"
                     /locus_tag="VI935_02090"
     CDS             1436..2638
                     /gene="trpB"
                     /locus_tag="VI935_02090"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HLE24422.1"
                     /translation="MKPKLKLPDEMGHFGIYGGRYVSETLMPALLELERAYLEIKDDP
                     KFKEEVNYYLREYAGRPTPLYFAERMTNELGGAKIYLKREDLAHTGAHKINNTIGQAL
                     LALRMGKRRIIAETGAGQHGVATATVAALLKMECEIFMGLEDTKRQALNVVRMKLLGA
                     KLREVASGTQTLKDAMNEAMRDWITNVRTTFYIIGSVAGPHPYPMMVRDFQSIIGEES
                     KRQILEKEERLPDLLIACVGGGSNAMGLFYPFLEDEKVKMIGIEAAGLGLETGVHAAS
                     ISAGRPGVLHGSKTYLLQNEDGQVKGTHSIAPGLDYPGVGPEHSFLKDSGRVSYTTVT
                     DKDALEGFMFLSEKEGIIPALESAHAVSYATKVAPTIPKDSIIIICLSGRGDKDIQVV
                     AKALGMNA"
     gene            2714..3526
                     /gene="trpA"
                     /locus_tag="VI935_02095"
     CDS             2714..3526
                     /gene="trpA"
                     /locus_tag="VI935_02095"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HLE24423.1"
                     /translation="MTRIHGKFKELKEKGKAALITYLTAGDPSLEATPDIIYKLEESG
                     ADIIEIGVPFSDPMADGPAIQLASERALKNGTTVKGVLDIVGKIRKRSEIPIITFGYY
                     NPFLSYGLRRFSEDARNAGVDGVLVVDLPPEEADEFKIHTDKAGLDLVFLLAPTSTSE
                     RIRLVSERASGFIYLVSVTGVTGERPAMDYSLESLVREIKNHTRLPVGIGFGVSTPDQ
                     ASKIANFADAVIVGSALVRIIEKYGRNGKGLLKEISTFINGLSQACFVVRGS"
     gene            3590..4918
                     /locus_tag="VI935_02100"
     CDS             3590..4918
                     /locus_tag="VI935_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HLE24424.1"
                     /translation="MQKELARYLFIGDFDSLLLGLFSKRGEVIWVSSLEEALNQTGNF
                     TVIMVEREHFSSIASKELSDHYPQTPIIVSNGEKPEEPPPYVRFVPFHKILIEEIARE
                     ERIARIELRVEELRRIHDEAKEMLILLHDNPDPDSIASALALRTLLKRNRQTAIIGHL
                     GEKISRPENVAMIDLLEIDLEKLVPPDIKEFDSIALVDVQPPYFGDLLPEVDTVIDHH
                     PYVGSYKARFSEIKTLEGATSTILTDFLRAAQVEISERLATALLYGIKTDTVSLNRDA
                     NPDDVEAFTFLYPLANLGLLRRIERAEAPPSEIKSFGRALANHWISEGIFFGNLGGVK
                     REYLIPKMADFGIQVKGIEWSVAYGIVSNSHLVISVRNVGYVKSAGRLVRTIFSELGS
                     AGGHRSAAKAIIPLKRVRKVLGKASQENIKKWVTELFINGVREKAGEEGS"
     gene            4994..5548
                     /locus_tag="VI935_02105"
     CDS             4994..5548
                     /locus_tag="VI935_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HLE24425.1"
                     /translation="MDFIQRDKISLVVIDMQEKLINAIPEERRETTIRNSKILIETAK
                     TFEIPITVTEQYPKGLGLTIPEIKDAVGDGFKPIEKLVFSCARSPEFKSALKDTKKEE
                     ALICGIETHVCVLQTVLDLINDGYKVYIPADGVTSRKELDWETGIKLIDKAGAIVGTT
                     ETFVFQLLERAGTEEFKKVSRLLK"
     gene            6057..6248
                     /locus_tag="VI935_02110"
     CDS             6057..6248
                     /locus_tag="VI935_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24426.1"
                     /translation="MENPYEELFKLMNLPMELSVKFFEVIEKGTKAMNSMAKAQKDME
                     EFQKAWKEFQELNPFLKLK"
     gene            6527..7087
                     /locus_tag="VI935_02115"
     CDS             6527..7087
                     /locus_tag="VI935_02115"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HLE24427.1"
                     /translation="MFLRKFFSDNHKRVLVFGINPGRFGAGITGVTFTDPVALETFCG
                     VPNDLPKKRELSSVFIYEFIKLWGGPKKFYRDFFLTAVSPLGFVKYGRNFNFYDHPAF
                     LRDIKPFLIRSIESQLGFGARREAAIVLGTGKIRQVFEDLNRECSFFKAVHALEHPRF
                     IMQYRRRKLRDYLRKYCEVFRQVLKE"
     gene            7284..7937
                     /locus_tag="VI935_02120"
     CDS             7284..7937
                     /locus_tag="VI935_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLE24428.1"
                     /translation="MNYVGVDLHQKTSWFYEVDSCGNKLDSRNVPNTEEDLKDYFGKI
                     PKPFTLAVESTCNWYFFVDLAQQYAEEVFLANSYELKAFAKRHKKTDKIDARLIANVL
                     HKGFLPAVTIADKQTRQIREIIRYRINIVRDRSRNVSRLKALLDKVGCHYSGDFTTYK
                     GLRRIDTEGLPLIHVYLISRYGEQIIELGQRIHDIEKQILHITEKDIDIELTHQKPI"
     gene            complement(7966..8997)
                     /locus_tag="VI935_02125"
     CDS             complement(7966..8997)
                     /locus_tag="VI935_02125"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HLE24429.1"
                     /translation="MASKLRLTILLLSSAVVWVAMPFQEATQAHPLAPSLLEIVERAP
                     GRVEVRWKTPATDVPGAELRPLLPPQCARSREIGVQVVGTGLIKRWEVECDAPLVESS
                     IEVRGISESKANVILRIALADGRKFSSVLSANRPSFIVPERKHSLIVLESYLVLGFKH
                     ILTGVDHLLFIFGLLLLIVGRRQLLFTITAFTIGHSITLSLAVLGFVKIPTAPVEALI
                     ALSILILAVELTRNTNAPPTLMRRFTWAMALVFGLLHGLGFAGALVQVGLPAGEIPLA
                     LFSFNLGIELGQLFFILVIFSAQALLRFVALPLPARSVLFPAYAIGSLSAFWLFERLS
                     RFLGLIYFE"
     gene            complement(8990..10060)
                     /locus_tag="VI935_02130"
     CDS             complement(8990..10060)
                     /locus_tag="VI935_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HLE24430.1"
                     /translation="MKRMINAFSRVIRAPALQFLFIGGLIFTANMWWKVLTQDNASRS
                     STEELVITAVHIEQLRRDWTGQTGFPPTPQQERALIEAAIDQEVLYREALALGLDRRD
                     IAIRRRLIQIARFVAEGSEQDEEALYRNPEQDDEAMYQKALELRLDRSDLVVRRRLVT
                     MMGLILERVPTEQAPSLFTDAELENYLKRHPERFIEPWHVQFTHVYLGEDRRGARAKE
                     DALRLLDKLRAQHIKPADAPGLGDPFLLGYHFLWKSRAEIEQLLGPRFASSITELQVG
                     VWLGPIPSSYGWHLVWVHATRPAGMQALDEVRNQVLQAVLKERRKQRLRDKLRQLRTR
                     YSVRVEYPVNSRGKLSGRDSDG"
     gene            complement(10079..12178)
                     /locus_tag="VI935_02135"
     CDS             complement(10079..12178)
                     /locus_tag="VI935_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HLE24431.1"
                     /translation="MKVWYKIVEAWVPIILLICLALPGVSRSEQNLPFKRTETREPCD
                     SFDPLRQPFFGETHVHTNFSFDAFAFSINNDPFDAYAFARFQKELELPPPIPSEPERT
                     ARIRKPLDFTAVTDHAELFGEMQICTRSSPDTPGIDSLECQLMREAGLNMPPPDPFNV
                     TAIWSLNPILRNFTARSLVMCEGPGVDCPGAAVSMWLDIQDAAEAFYDRSSECKFTTF
                     VAYEYTAQPSFDNLHRNVIFRNDKVPESPISNVTTGGPFIPVLWQKLREECLEAGTGC
                     DVLTIPHNPNLSGSTREDPAGRMFPDPRSVDEARERAFFEPLVEIIQHKGASECRFDR
                     IARMGVQTVDELCTFEQVLPDTLSTTAPSVPIDEFPTRDMVRNALKDGMALAPNFDGI
                     NPFKYGLIGSTDDHNGAPGSTLEPDFQGHGGTNDAPLARMIDKFYLGPGGLAVVWAEE
                     NSRDSIFEAMLRKEAYGTSGTRPVVRFFGGWEYDTRPNKLCNQVNRVEIGYEKGVPMG
                     SDLPPLVDGTNPRFLVAAMKDPGYPAVLPGQEPIRGTPLQRIQIVKGWVDSDGVTHEK
                     VVNVAGNANNRAKVKKKTCEPKGAGFKELCTVYEDKDFDPTQPAFYYARVLENPTCRW
                     STFACKAAGVDPFSRQKKCQRQAAVANAKAVEAGEIEEGDTPFNNCCLNEKNDPFLDQ
                     TLQERAWTSPIWYVPEE"
     gene            complement(12348..13304)
                     /locus_tag="VI935_02140"
     CDS             complement(12348..13304)
                     /locus_tag="VI935_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLE24432.1"
                     /translation="MARALRLAFEGACYHITSRGIRREDIFFSDNDKKVFIEKLNETF
                     NKYRFVCYAYCLMDNHYHLFIKTPHAKISEGMHYLNTSYTNWLKAEHNIVGTVLQGRY
                     KSMLVDQDNYGLKLSAYIHLNPLRAGMVKDLKQYRWSSYLDYVSDRMKMVERLDTEFI
                     LSQFGEDPKDARKRYRSYVKENTEMKDPLVESYRGIALGTEGYINKIIDRMKKIGRKR
                     EIVATRLSGAKNAQEMIGLIQNRYGVTRNDIVDRKRGNVYRKISMYLIKKHTDLRLSE
                     IGRIYGMDYAGVSQSCKRFGEEIKRDKKIRRTIEEIEREMLV"
     gene            complement(13429..13788)
                     /locus_tag="VI935_02145"
     CDS             complement(13429..13788)
                     /locus_tag="VI935_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HLE24433.1"
                     /translation="MNSNIYWILELQVQTGRESDFRVLMTEMVSATLANEPGTLNYEW
                     STSADGKLCHIYERYVDSAAVMTHLGTFGERFAARFLEVLKPVRFVVYGSPSTAVKDA
                     LAGFNPIYMQAVGGFSR"
     gene            complement(14491..14655)
                     /locus_tag="VI935_02150"
     CDS             complement(14491..14655)
                     /locus_tag="VI935_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24434.1"
                     /translation="MKLLTDEPQGNDITVKVNAENKERISSVQTKPDRFTIGKDALNP
                     PNVSYERNFP"
     gene            14930..15295
                     /locus_tag="VI935_02155"
     CDS             14930..15295
                     /locus_tag="VI935_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24435.1"
                     /translation="MEVNTRIAERFVLRGKEVYLSKKDIERVAQNGISGRGTAYFALI
                     GGKRIPVKNLLQEALKDKGFYFSLLEFTTQDAVRILGKLGVEIIEDRRVGTAKSLLRF
                     AGVVSMGGDAVEDEKGLYE"
     gene            15292..15687
                     /locus_tag="VI935_02160"
     CDS             15292..15687
                     /locus_tag="VI935_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLE24436.1"
                     /translation="MIVIDTNIIVALLDNKDTHYKHAVRILEELGDERVYILNVNLAE
                     VYSVIARRCRERRYNCKDALGKLRELEANINVIWIENLKETHVEIVDRVIESEGKLNY
                     NDAALLKYVRNKGTKLITLNRELLRGLNR"
     gene            complement(15927..16718)
                     /locus_tag="VI935_02165"
     CDS             complement(15927..16718)
                     /locus_tag="VI935_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLE24437.1"
                     /translation="MTTKAITVVNSIDNAELEKKVKEMYREVALNLHGEFHFEMGRAL
                     AERLGYLSEDLDRIPAEAIGSFAGVGYFFHLANLKEGESVIDLGSGSGMDTFVAALKV
                     GENGNVVGVDMTDEQRAKAERLRDQAGFNNVTYIKGYIDTIPCADNGFDAVISNGVIN
                     LASDKGKVFQEAARILKPGGRLAISDIVTEVQLPNGITCNTTLWASCIGGAMQQDSYR
                     AAIEAAGLRVEKVQENPQYQFISENARGASNKFGVKSISLLAVKE"
BASE COUNT         4741 a         3709 c         3796 g         4519 t
ORIGIN      
        1 attcatcccc cgtttcagga actgtcttgg aggtgcgtag gtttgagatt tcacctagtt
       61 tttctggtag ggagtttgtc taccgaatag gtgatctgag ttacgaatca gacttttata
      121 atcaattttt tagacctcct aacttactta ctacagatga agtgcgtaag tggttttctg
      181 aatcaggcct gtttaaaaac gttgtagtcc catcaaataa tgtggagtct gactatgtcc
      241 tagaaggcaa tgttagcgaa ttatatggtg attttagagc aactaatgaa ccaaaagcag
      301 ttttagggat tcagttcttt ctgacagaag agacttctac caatcctaaa atagtattcg
      361 aaaataatta tagaagagaa gtcgttctcc gttctaattc agctcaggat ttagtcaaag
      421 gttggaatga agcgcttcag caaatcttga cagctcttga aggtcatctt aaaaatgtag
      481 atttcaagac gcctaaataa agccatttaa gtaaacaaac ctataaattg ggatgcacta
      541 gtgtataaaa ttagcaccga aaacggattt attatttccc tttaaatagg agccaaacta
      601 cagtagagga acttgaaatc ccctctgact cccctttaga aaaggggaga aaaataaatg
      661 tcccactttg taaaggggga tttaggggga tttaaatttt cattaatatg ctctaatacc
      721 ccctctatta taattacact tttatcgaca ctgtctgcca ttataaatct tgtccctttc
      781 aatttatttt acatatggag tttcaacttt tataattatc cctctgtaaa tatggtcagg
      841 ataaaaatct gtggaatcac taatatagag gatgccctgt tatctgtaga cttgggtgcc
      901 aacgccctgg gatttgtgtt ttatcaggga agcaaaaggt atattaagcc tgataatgct
      961 caaaccataa tttcaaaatt acccccattt gttacaactg ttggagtctt tgtgaatcag
     1021 atattagaag acataataaa aataaaagag aaggcaggat ttgatgcctt tcaactccac
     1081 ggagaagaat ctccggattt ctgtatgaaa ttaaaaggaa aggtaataaa agccattcgg
     1141 gttaaagata gcatagaccc aaaggagatt gagtcctatc ctactcaagc tattctcttt
     1201 gataactact caatggagta ttatggagga gcaggagaaa ccttcgggtg ggaggttcta
     1261 agaggcttag atacatcaaa aaaaataatt ctttcaggtg ggcttacttc agaaaacgtc
     1321 gctaatgcaa ttaggatagt aaatccctat gcggtcgatg ttagcactgg tgttgaggaa
     1381 attcctggaa agaaagaccc agggaaatta aaaagattta tagaggcggc aaggaatgaa
     1441 acctaaacta aaacttcctg atgaaatggg acactttgga atctatggag gcagatatgt
     1501 atcagaaacc cttatgccag cacttcttga actagaaagg gcttatctcg agattaaaga
     1561 tgacccaaaa tttaaagaag aagtaaatta ttaccttaga gaatatgctg gccgccctac
     1621 accgctctac ttcgcagaaa gaatgacgaa cgagctaggt ggggctaaga tctacttaaa
     1681 acgcgaggac ttggcgcata caggcgcaca taagattaat aacactattg gacaggccct
     1741 tctcgcatta cgtatgggaa aaaggaggat tatcgcagaa acgggagcag gacagcacgg
     1801 ggtagctacg gcaacagttg cagcgctcct taaaatggaa tgtgaaatat tcatggggct
     1861 tgaggataca aaaaggcagg cacttaatgt tgtgcgcatg aaactacttg gagcaaaatt
     1921 aagggaagta gcttcaggca ctcagactct aaaggatgcg atgaatgagg ctatgaggga
     1981 ttggataaca aatgtgagga caactttcta catcatagga agcgtggcag gacctcatcc
     2041 ctatccgatg atggtaaggg attttcagtc aataatagga gaagaatcaa agagacagat
     2101 tctagaaaaa gaggaaaggc ttccagacct tcttatcgcc tgtgttgggg gtggttctaa
     2161 tgcaatgggg cttttctacc cttttctcga agacgaaaag gtaaagatga taggaattga
     2221 agccgcaggg ttagggcttg aaacaggtgt acatgccgca agtatttctg caggcaggcc
     2281 cggggttctt cacgggagca aaacctattt acttcaaaac gaagatggcc aggtaaaagg
     2341 cacccattca atcgcccctg gacttgatta cccaggggta ggtccagaac actccttctt
     2401 aaaggattcg gggcgggttt catacacaac ggtcaccgat aaagatgccc tagaagggtt
     2461 catgttcctc tcagaaaaag agggaataat ccctgcgcta gagtcagcac atgctgtttc
     2521 ttatgcaact aaagtagctc caactatccc caaagattca ataattataa tttgcctttc
     2581 agggcgcggg gacaaggata ttcaggtcgt agcaaaggct cttggaatga acgcataggg
     2641 cttgaggttt ccatcctatc ggatagaatt aagaggggta tttcctagac gataacaacc
     2701 aaagcttaga tatatgacca ggatccatgg caagtttaaa gaacttaagg aaaagggaaa
     2761 ggcggcttta attacctatc tcacagccgg tgacccatcg ttagaagcaa ctcccgatat
     2821 aatctataag cttgaagaaa gcggtgctga cataatcgag attggcgttc cgttttcaga
     2881 cccaatggca gatggtcctg caatccaact cgcctccgaa agggcgctca agaacggcac
     2941 aacagttaaa ggagtgcttg atatcgtggg taagatcagg aaacgctcag agatccccat
     3001 tataactttt ggatattata atccttttct ttcctacggt ctcaggagat tctcagaaga
     3061 tgcgcgcaac gccggagtag atggagtatt agtagttgac cttcctcctg aagaagcaga
     3121 tgaatttaag attcacactg ataaagcagg gcttgacctt gtgtttcttc ttgctcctac
     3181 aagcacatcc gaaagaataa ggctcgtctc agagcgtgca agcgggttta tctatctcgt
     3241 ttctgtaaca ggtgttactg gagaaaggcc agccatggat tattcacttg agtcccttgt
     3301 tcgtgaaatc aagaatcata caagacttcc tgttggaatt gggtttggtg tgtcaacacc
     3361 ggatcaggct tcaaagatag ctaattttgc agatgctgtg attgttggca gcgcacttgt
     3421 gagaataatt gagaaatatg gacgtaacgg gaaaggacta ttgaaggaga tttccacatt
     3481 tataaatgga cttagccagg cgtgtttcgt agttcgtggt tcgtgattcg tggttcgata
     3541 cacgatacac gattccagag ccacgattca ctattgtgga ttagaaagca tgcagaaaga
     3601 actcgcgaga tatttattca taggagattt cgattcgctt cttctaggac tcttttcaaa
     3661 aaggggtgag gtaatttggg ttagctcact cgaagaggca ttaaatcaaa cgggcaactt
     3721 tacagttatc atggttgaaa gagagcactt ttcaagcatc gcatctaagg agctttccga
     3781 tcactaccca cagacaccga tcattgtaag caacggtgaa aaaccagaag agcctccacc
     3841 ttatgtgagg tttgttcctt ttcacaaaat tctcatagag gagatcgcac gtgaagaaag
     3901 gattgctaga atagaactcc gagtcgaaga actaaggcgc attcacgatg aagcgaaaga
     3961 gatgcttatc cttcttcatg ataaccctga cccagattcg attgcaagtg cactagcgct
     4021 tagaacccta cttaaaagaa accgccagac tgccataatt ggacaccttg gtgaaaagat
     4081 atctcgtcca gaaaacgttg ccatgataga cctactcgaa attgacctag aaaaactcgt
     4141 acctcctgac ataaaagagt tcgattcaat tgcattagtt gacgttcagc caccttattt
     4201 tggagatctt cttccggagg ttgatacagt gattgatcat catccttatg ttggttcgta
     4261 taaggcccga ttttcggaaa taaaaaccct ggaaggagct acttctacca tacttactga
     4321 ttttcttcgt gctgcgcagg tagaaatatc agaaaggtta gccactgccc ttctctatgg
     4381 cataaaaact gacactgtat cccttaaccg tgatgcaaac ccagatgatg tcgaggcatt
     4441 tacctttctt tatccactcg caaatctagg cctattaaga cgaatcgagc gtgcagaggc
     4501 tccgccctca gaaatcaaat ctttcgggag ggcccttgct aatcactgga tatcagaagg
     4561 catctttttt ggcaatctcg gaggagtaaa aagggaatat ttaatcccaa agatggctga
     4621 tttcggaatt caggtaaagg ggatagagtg gtccgtggct tatggaatag taagtaattc
     4681 acatctggtt atttcagtaa gaaatgttgg atatgtaaaa agcgcaggga gacttgtaag
     4741 aaccatcttt agcgaacttg gtagcgcagg tgggcatagg tctgcagcca aggctattat
     4801 tcctctaaaa agggttagaa aagtccttgg caaggcttct caagaaaaca taaaaaaatg
     4861 ggttaccgag ctttttatca atggtgtcag agaaaaggct ggagaagagg gctcgtgaat
     4921 cgtgatttgt ggattgtcta tcgtatcaca agtcactaac cacaaaccac tatccacaaa
     4981 tagggaggtt gagatggatt tcattcaaag ggataagatt tcgctcgttg tcattgatat
     5041 gcaggaaaag cttataaacg ccattccaga agagagaaga gaaacaacta taagaaactc
     5101 caagatctta atagaaacgg caaagacctt tgagattcct attacagtaa ctgagcaata
     5161 cccaaaggga ttaggactta caatccctga gatcaaagat gccgttggag atggattcaa
     5221 accaattgaa aaactcgtgt ttagctgcgc tagatcccct gagtttaaat cggcattaaa
     5281 agatacaaag aaagaagaag cgctaatatg tgggattgaa acacatgttt gtgtactcca
     5341 gacggtgcta gatttaatta atgatgggta taaagtttat attcctgctg atggtgtaac
     5401 atcaagaaag gaactcgatt gggaaaccgg aataaagctc attgataaag ctggcgccat
     5461 tgtagggaca accgaaacct ttgtgtttca actcctcgaa cgcgcaggga cagaggagtt
     5521 caagaaggta tcaagattgc ttaagtaact tacaaaaccg tttaaaacta aagcaatcac
     5581 acctactgaa acctttatta caaactacct acggaggttt aataccacag ctcctccagg
     5641 gctattattt tctataaggc acgcctaatc atagtcatga atagggtgat tagggcaatc
     5701 aaccgtgaat gggtaggttt aacaatatta tgcaacctat agaaacgtcc atcgcccagt
     5761 gaggcactag accccagcac atcatgttta atactctact atcttcatta actatcaaac
     5821 tggggaattc taagatgtag cgataatgtt atccttcagc agacgaaaaa ataagtttat
     5881 catttatgga gtatgtaatt caatgagggg ggaaggcaga aaataaaagt ttttgaagtt
     5941 tgcataaaaa agctaaggca aaaatcaagg cttgatacat tctcccattt acgagtagtt
     6001 taaaatagtt attatttatt gatattaatc ttaaaaatct gaaaaaggag cctattatgg
     6061 aaaatccata tgaagaatta tttaaattga tgaatctacc tatggaatta agtgttaagt
     6121 ttttcgaggt gattgagaag ggtaccaaag ctatgaattc aatggcaaaa gctcaaaaag
     6181 atatggaaga gtttcaaaag gcttggaagg aatttcagga attaaatcca tttttaaaat
     6241 taaaataaaa aaaaggttgt ctcacccgtt cccccttttt tctcttttta cactatcagg
     6301 caaaattgat agtctggatc ttaatcttgt gtattttgtc attaactgat agcctgaaaa
     6361 ccgaggcttg gttcgattcc tatgtattct cattatgaaa cacttatcgg cattcggtga
     6421 acgcgcgctc cgattctata cctctcttga actacgcaac aaaccgccgc ggggcgtggt
     6481 cgccatgaat ccatatcgca ctccgcgcaa tagagattat ataagattgt ttctacgtaa
     6541 gttcttttcc gacaaccata aacgagtgct tgtctttggg attaatccag gaagatttgg
     6601 tgccggcatt actggtgtca ctttcaccga tccagtagcg ctcgaaacgt tttgtggtgt
     6661 tccgaacgac ttaccaaaaa agcgggaact ttcgtccgtg tttatttatg agttcatcaa
     6721 attatggggc ggtccaaaga agttttatcg agattttttc ctaacggctg tttctccgtt
     6781 agggtttgtc aagtacggca ggaattttaa cttctatgat caccccgctt ttcttcgcga
     6841 catcaaacca ttccttatcc gctcgatcga atctcaatta ggattcggag cacgacgcga
     6901 agcagccatt gtgctcggca cgggaaagat taggcaggtc ttcgaagatc tcaaccgcga
     6961 atgtagcttt ttcaaagccg tccacgccct agagcatcct cggtttatca tgcagtaccg
     7021 ccgtcgaaag cttagagatt atctccgaaa gtattgtgag gttttcaggc aagtgctcaa
     7081 agaataaatt ttagtaaatt ttagggttat agtaaatttt agggttaggt ctttaattgt
     7141 gcattcttgg tttataatca actccaccgt tacacaaaat cttatatttc aagacctgct
     7201 aatatgagag gtgattcctc gcaaagctgt tagaattgga ttggaaactt aggtttttaa
     7261 tccaacaaca aggaggaatc accatgaact atgtaggagt tgatcttcat cagaaaacat
     7321 cttggttcta tgaagttgat tcttgtggta ataaactaga cagtagaaat gtaccaaaca
     7381 ccgaagagga tttaaaggat tattttggga agataccgaa gccatttaca cttgcggttg
     7441 aatccacttg caattggtat ttctttgtag acttagccca gcaatatgct gaggaggtgt
     7501 ttttggctaa ttcttacgaa cttaaagcat ttgctaagag acataaaaag acagacaaaa
     7561 tagatgcccg attaatagct aatgtacttc acaaaggatt cctgccagct gtaacaattg
     7621 cagataaaca gaccagacaa ataagagaga taattcgtta tcggataaac atagtaagag
     7681 atcgctcgag aaacgtatct cgtcttaaag ctttactgga taaggtaggt tgtcattatt
     7741 caggtgattt tacaacttat aagggtttga gaagaataga cacagaaggt ttacccctca
     7801 tccacgttta tttaataagt agatatgggg aacagattat agaattaggc cagaggattc
     7861 atgatattga aaaacagatc ttgcatataa ctgagaaaga cattgacatc gaattgactc
     7921 atcaaaaacc tatctaaaat agtatctttg ccttatataa aaaatctatt cgaaatagat
     7981 caaccccaaa aatcttgaca accgctcgaa caaccaaaac gccgagagtg agccgattgc
     8041 gtaagcagga aacaacacac tcctcgctgg taagggcaaa gctacaaacc taagcaaggc
     8101 ttgagcacta aagatcacta ggatgaagaa aagctgaccc agttcgatgc cgaggttgaa
     8161 tgagaagagg gcaagaggaa tttcacctgc aggtagacca acctgcacca aagcaccggc
     8221 gaaaccaagt ccgtgaagga ggccaaagac caaggccatc gcccaggtaa agcggcgcat
     8281 tagagtgggg ggagcattag tattacgggt gagctccaca gccagaatca gaatgctgag
     8341 tgctatcaga gcctcaacag gtgcggttgg aattttcaca aacccgagga ctgcaagcga
     8401 aagagttata ctgtggccaa ttgtaaaggc agtaatggta aataagagct gacggcgacc
     8461 gacaatcagt aacagtagac caaatataaa gagtaggtga tccacccctg tcaatatatg
     8521 cttgaaaccc aggaccagat agctctccaa cacaataaga gaatgcttgc gctcaggcac
     8581 tataaacgaa gggcggttgg cgcttaacac gctactaaat tttcggccgt cggccagggc
     8641 gatccgtaga atgacgttgg ctttgctctc tgaaatcccc ctgacctcaa tggagctttc
     8701 cacaagaggt gcatcgcatt cgacctccca gcgcttgatg agccctgtcc ccacaacttg
     8761 cacaccgatt tcccgagacc gggcacattg aggtggtagc agtgggcgca attctgcacc
     8821 cggcacgtcg gtcgcagggg ttttccaccg aacttccacg cggcccgggg cacgctcgac
     8881 gatctccagt agagacggcg ctagaggatg cgcctgggta gcctcctgga atggcatcgc
     8941 gacccagact accgcgctgg atagcagcag aatggtgagc cgtaatttgc tagccatcgg
     9001 aatccctccc tgagagcttg cctcggctgt tcacgggata ctcaacgcgc accgaataac
     9061 gggtacgtag ttgcctcagc ttgtctcgca accgctgctt ccggcgctcc ttgagtaccg
     9121 cctgaagcac ctggttacgc acctcgtcca atgcctgcat tccagccggc cgtgtcgcat
     9181 ggacccagac caggtgccag ccgtagctgg agggaatcgg ccccaaccac acgccaactt
     9241 ggagttctgt tattgaactg gcgaagcgtg ggcccagaag ttgctcgatc tccgcgcgcg
     9301 atttccagag aaagtggtaa cctagtagaa agggatcgcc caggcccggc gcgtcggcgg
     9361 gcttgatgtg ctgcgctcgg agtttatcta gtaagcgaag tgcgtcctcc ttagcccgag
     9421 caccgcgcct gtcctcaccc agatatacgt gggtgaattg tacgtgccag ggctctatga
     9481 agcgttcggg atgccgcttg aggtagtttt cgagttctgc atcggtgaat agcgagggtg
     9541 cctgctccgt aggcactctt tctaagatga ggcccatcat ggtcactaac cttcgtcgca
     9601 cgacgaggtc gcttcgatcg agccggagtt ccagagcctt ctggtacatg gcttcgtcgt
     9661 cttgctccgg gttccggtac agcgcttcct cgtcctgctc cgagccctcc gccacaaatc
     9721 gcgcgatctg tatgaggcgg cgccgaatgg caatgtccct tctatcaagg cccagggcga
     9781 gcgcctctcg atagagcact tcctgatcta ttgcggcttc gatgagagcc cgctcctgct
     9841 ggggagtcgg agggaacccg gtttgtccgg tccaatcccg tcgaagctgc tcgatatgga
     9901 cagccgttat caccagttcc tcggtagagg accgactggc attgtcttgg gtcagaacct
     9961 tccaccacat atttgcggtg aatatcagcc caccgatgaa gaggaattgc aacgccggag
    10021 cacggattac tcgggagaaa gcgtttatca ttctcttcat ttttttctca tccttggact
    10081 actcctcggg cacgtaccag ataggtgaag tccaggcgcg ctcctgtagg gtctgatcta
    10141 ggaagggatc gttcttctcg ttcagacagc aattattgaa gggggtatca ccctcctcaa
    10201 tttcacccgc ctctactgct ttcgcattcg ccaccgcagc ctgccgctgg catttttttt
    10261 gtctggagaa gggatcaacg cctgccgctt tgcaagcaaa tgtgctccag cgacaggtgg
    10321 ggttttcgag gacgcgcgcg tagtagaacg cgggctgtgt cggatcaaag tctttatcct
    10381 catacacggt gcacaactct ttaaagccgg cacccttggg ctcacaggtt ttctttttaa
    10441 cctttgcgcg gttattcgca ttaccagcca cattcaccac cttttcatgg gtcacaccat
    10501 cactgtctac ccaacccttg acaatctgga tgcgctggag cggggttccg cggataggct
    10561 cttggccagg gagaacagcc gggtagccag ggtccttcat ggctgcaacc aggaaacgcg
    10621 gattagtgcc atccacaaga ggaggcagat ccgatcccat gggcacgccc ttctcatatc
    10681 caatttcaac gcggttcact tggttgcaca gcttgttcgg cctcgtgtca tactcccacc
    10741 ccccgaagaa acgtactacg gggcgtgtgc cgctggtgcc ataggcctcc tttcggagca
    10801 tggcctcaaa gatggagtcg cgggagttct cctcggccca gaccacagcg agcccgccag
    10861 gtccaagata aaatttatca atcattcgcg caagaggagc gtcgttggtg cccccgtggc
    10921 cctggaaatc gggttctagt gtggatcccg gagctccgtt gtggtcgtca gtactgccaa
    10981 tcaatccgta cttgaatgga tttatcccgt cgaagttcgg tgcaagtgcc atcccgtcct
    11041 tgagggcgtt gcggaccata tcccgtgtcg gaaattcatc aatgggcact gagggtgcgg
    11101 tcgtggaaag cgtgtccgga agcacctgct caaaggtgca gagttcgtca acagtctgga
    11161 cgcccatcct agctatccga tcaaagcggc attccgatgc gcccttgtgc tgaatgattt
    11221 cgacgagtgg ctcgaagaag gcgcgctcgc gagcctcgtc gacgctccgc gggtcgggga
    11281 acattcgccc ggcagggtcc tcacgagtac tgcccgaaag gttaggattg tgaggaatcg
    11341 tgaggacgtc acaacccgtt ccagcttcca ggcattcctc tctcagtttc tgccacaata
    11401 ccgggataaa gggtccacca gtcgtgacgt tgctgatagg actttcgggc accttatcgt
    11461 tcctgaagat tacgttgcgg tggagattgt caaatgacgg ctgcgccgtg tattcgtatg
    11521 ccacgaaggt tgtgaactta cattcggagc ttctatcata aaatgcctcg gcagcatctt
    11581 ggatatccag ccacatcgaa accgcggcac cagggcagtc cactccgggt ccttcgcaca
    11641 tgacaaggga acgcgcggta aaattcctta aaataggatt taaactccag atcgctgtca
    11701 cattaaacgg atcggggggc ggcatattga ggcctgcctc ccgcatcagc tggcactcta
    11761 aactgtcgat tccaggagtg tcaggggaag agcgggtgca tatctgcatc tcgccgaaga
    11821 gttctgcgtg atcagtgact gcggtgaaat ccagcggctt gcggatccgg gccgtccgct
    11881 ccggctcgct cggtatgggt ggcggaagtt cgagttcttt ctggaacctc gcgaaagcat
    11941 aggcgtcaaa tggatcatta ttgatcgaga aagcgaaggc gtcgaaggag aagttcgtgt
    12001 gaacgtgtgt ttcgccgaag aaaggctgac gcagcggatc gaaagagtca cagggctctc
    12061 gggtctcggt cctcttgaag gggaggttct gttccgatcg gcttactcca ggtaatgcca
    12121 aacaaataag caggataatc gggacccaag cttctacaat tttgtaccat actttcacga
    12181 tatacctccc aacatccaga gcctgacgat aattatggcg aatgaattga tctttgttaa
    12241 tttttatttc aatattttaa tattttaata aaggactaaa ggggtcacgc ttttgcattt
    12301 ttgtttaaac tttggaaact ttggggtcgt atctcaactt tcaacattca gactaacatt
    12361 tccctctcta tctcctcgat tgtccttctg atttttttat ccctcttaat ttcctctccg
    12421 aacctcttac agctctgtga aacacccgca taatccatac catatattct acctatttca
    12481 cttaaccgta aatcagtatg cttctttatc aaatacatcg atattttcct atacacattc
    12541 cccctcttcc tatctactat atcgttcctt gttactccat atctgttttg tattaatcct
    12601 atcatctcct gtgcattttt tgccccactt agccgtgttg ctactatttc tctttttctc
    12661 ccaatcttct tcattctatc tataatctta tttatatatc cctcagtccc tagtgctata
    12721 cccctgtagg attccaccaa tggatccttc atttcggtat tttcctttac gtaactcctg
    12781 tatctcttcc ttgcatcttt tggatcttct ccaaactgac tcaatatgaa ttctgtgtcc
    12841 aatctctcta ccattttcat cctatcactc acataatcca gataactgct ccatctgtat
    12901 tgctttaaat cctttaccat acctgcccta aggggattta ggtgtatata tgcactgagc
    12961 tttaacccat aattgtcttg atctactagc attgatttat atctcccctg cagtacagtg
    13021 cccactatgt tgtgctctgc cttaagccag tttgtatacg aggtgttcag ataatgcata
    13081 ccttctgata ttttcgcatg tggggtcttt ataaacaaat ggtagtgatt gtccataaga
    13141 caataggcat aacagacaaa acggtattta ttaaatgtct cgttaagttt ctctataaat
    13201 actttcttat cattatctga aaaaaatata tcttctcttc taatgcctcg ggaagtaata
    13261 tgataacatg caccttcaaa tgccagtctc aaagcccttg ccataagatc attataaaat
    13321 aacatccgtt gtttgtcaat tgttgagata cgacccctca agtcggcagg gcctctggtc
    13381 gcagcaggga cgtccgttgc gcggtcgcac cggcgccggt ccgtcgcgct atcggctaaa
    13441 gccgcctacc gcctgcatgt agatagggtt gaatccggcc agcgcatcct tgaccgccgt
    13501 gctcggtgag ccgtatacga caaagcggac gggtttcaat acctccagaa agcgggcggc
    13561 aaacctctca ccaaacgtgc ccagatgagt catcactgcc gcggagtcaa catatcgctc
    13621 atagatgtga cagagcttgc catccgcgct cgtgctccac tcataattaa gcgtgccagg
    13681 ttcattcgct agggtggcgc tgaccatctc tgtcatgaga acccggaagt cactctcccg
    13741 ccccgtttga acttgcagct ccaaaatcca ataaatgttg ctgttcatgt tgcctctctc
    13801 ggtgaagggt aaggtcgccg acccctgccc ggcgtcggcg ttgcctaact actgattata
    13861 cggatttttt tatataattc cctatataag gtattatact gaaatcctcg taatctctat
    13921 cctcgctgtt cattatataa caagcgaccc cgcctaagca acgccttccc tctctataca
    13981 aaatatacgg atatacaact ctaggtccca gcaacgtcag atgtcaaatt cctcatcgga
    14041 gtcctcttag cagttcctag aataaaaaac aggcctgtaa ataaacccgt tcttgatagc
    14101 gttactatag attaaagatt ttctttcgtc gcacactatc acccaaaaag ccaaagatcg
    14161 ttagtatttc aacttatttc taccgaacca atacaccgac tctcgagtgg taaaatcatg
    14221 ccatattctt gctatatagc gctaccacta tgcgcgtgtg gtaagggtaa tttcttgtac
    14281 ctgaacttat tcttaatatg tgattgagtt ttgaaagttg ataaggtgaa tttttcggcg
    14341 actggatctg actcatccca aaaagagcgt catggaacag cccccctagc cgatatctgt
    14401 ccatttgtac gttgcgacgt ggtcgttacc atcttggtct ctaacgttga cggttactgt
    14461 gtgtcttctc cctggtctag ccgagccttg ttaaggaaaa tttcgctcat atgaaacatt
    14521 cggagggtta agcgcgtctt tgccaatggt aaagcgatcc ggcttggttt gaacggagga
    14581 aattctttct ttgttctcag cgtttacttt gactgtgatg tcattccctt ggggctcgtc
    14641 tgtgagtaat ttcaattttc atatcttctc taacgactta atttgatcag tttcaataac
    14701 gacaatgcat catcgttaca tttttccaac tcccccatgc attactattg cttgaatatt
    14761 aaataagtag tgtatttata catatattac accacaacac aaaagccaga aaaacggtgg
    14821 tatggtgcct ctactttcct gattttgtaa gccatcctac acacataata aaactgctcc
    14881 tgactattga ctaagcagca aataaacata cacttatagc aatatataag tggaggtaaa
    14941 tacgaggata gcagaaaggt ttgtgttgag aggtaaagag gtttacctat caaagaaaga
    15001 tattgaaagg gttgctcaaa atgggatttc aggaagaggt acagcctact ttgctttgat
    15061 tggcgggaag agaataccgg taaagaattt gcttcaagaa gctcttaagg acaagggttt
    15121 ttatttctca ctcctcgaat tcacaactca agacgctgtg agaatacttg gaaaacttgg
    15181 ggttgaaatt atagaagaca gaagagtagg cactgcaaaa agtcttttga ggtttgcagg
    15241 ggtggtgtct atgggaggtg atgcagtaga agacgaaaag ggactttacg aatgatagtg
    15301 attgatacaa acattattgt ggctcttcta gataacaaag acacccatta caaacatgca
    15361 gttaggatat tagaagagct aggagatgag agagtatata ttcttaatgt aaatcttgca
    15421 gaggtatact ccgtgatagc gaggaggtgt agagaaagga gatataattg taaagatgca
    15481 ctagggaaat tgagagagct tgaggcaaat ataaatgtga tatggattga aaacctaaag
    15541 gaaactcacg tggaaatagt ggatagggtt attgaaagcg aagggaagct taattacaac
    15601 gatgcggcgc tattaaagta tgttaggaat aaaggaacta aactgataac gttaaataga
    15661 gaacttttaa ggggattaaa tcggtagtat atagataacc ctagtatacc aaccgcctga
    15721 aaagccagaa aacgatagta tttgggttat ctacgaggaa attaatttag cgaaattatc
    15781 gatggtcgcg cccaacgcca cacttaagcc gagtgataga gcgttggctt gaagtgcttg
    15841 ttaggtttcg gccaatgttc gtacaaaatg aaaagggttg ctctccttat aatttctaca
    15901 cccaagatga ttgacgcact tcaatattat tccttcactg ctaacagaga tatacttttc
    15961 acgccaaact tattgcttgc tccccgggca ttctcggaga tgaattgata ctgcggattt
    16021 tcttgtactt tctctacgcg caaacctgcc gcttcaatcg ccgcacgata actatcctgt
    16081 tgcatagccc ctccgatgca ggaggcccac aatgtggtat tacaggtaat gccattcggg
    16141 agctgcacct cggttacgat gtccgagatc gcgaggcgtc cgccaggttt tagtatccgt
    16201 gcagcctcct gaaacacctt tcctttgtca gaggcgagat tgattactcc gttgcttatt
    16261 acggcatcga aaccgttgtc tgcacaaggg atagtatcga tgtaaccctt gatataagtg
    16321 acgttgttga atcccgcctg gtcacgcagg cgctcagcct tggcccgctg ttcatctgtc
    16381 atgtcaactc ctaccacatt gccattttca cccaccttca aggctgcgac aaaagtatcc
    16441 atacccgatc cgctaccgag gtcaattacg ctttcacctt ctttgagatt tgctagatgg
    16501 aaaaagtaac cgacaccggc aaacgaaccg atcgcctcgg ccgggatgcg gtctagatct
    16561 tctgataaat agccaagtct ttccgccagg gcgcggccca tctcgaagtg aaactctcca
    16621 tgtaggttta gcgcgacctc gcggtacatt tccttaacct ttttttcaag ttcagcatta
    16681 tcgatcgagt taacaacagt aatagctttt gtagtcatat gttctcctat cgttttgaaa
    16741 taggcttctg atttctatcc taggg
//
LOCUS       DATDRL010000061         2272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3405473, whole genome shotgun sequence.
ACCESSION   DATDRL010000061 DATDRL010000000
VERSION     DATDRL010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3405473"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /gene="lepA"
                     /locus_tag="VI935_02170"
     CDS             <1..1587
                     /gene="lepA"
                     /locus_tag="VI935_02170"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HLE24438.1"
                     /translation="GTTYEFNLIDTPGHVDFSYEVSRSLTACEGVLLVVDASQGVEAQ
                     TLAHAYLASDSGLELIPIINKIDLPAADPERVKKEIEEAIGISAEEAILASAKEGIGT
                     KEILEAIVKKIPIPLGSGDSPLKALIFDSWFDSYQGVVMLVRVFEGRIKLGMKIQLMS
                     TGKAYEVKKLGIFAPRALEVDEISAGEVGFVTAAIKEISEAKVGDTITDALKPTDAPL
                     TGFRLMKPMVFSGLYPIDPGEYDKLRDALQKLRLNDSAFIYEPETSLALGFGFRCGFL
                     GLLHMEIVRERLEREFELELISTAPTVVYMVTDTKGEEIYVDNPSKLPSSEKIETIEE
                     PYVLVMLHTPSAYLGAIFELCENRRGTQKNIKYITQDRVIVEYELPLSEIMFDFFDRL
                     KSVSRGYASMDYELIGYKTSELVKLDVLINGEPVDALSIIVHKEKAYERGKELVEKLR
                     KLIPRQLFEVALQAAIGNRVIAKETVKPVRKDVTAKCYGGDITRKRKLLEKQKEGKKR
                     LKQIGKIEIPQEAFLAVLKT"
     gene            1910..>2272
                     /locus_tag="VI935_02175"
     CDS             1910..>2272
                     /locus_tag="VI935_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24439.1"
                     /translation="MTKVVSFSLVTVLLFSFAAYGFSGKHDGKHGKSNWWNNTKVLEQ
                     LKLSDQQKTKIGGIASSHKETLENLRAQIKTAHEKLNESVKNPNSTKDRILAEFDQLE
                     KTRGELRKVEFEMSLEMRD"
BASE COUNT          760 a          433 c          492 g          587 t
ORIGIN      
        1 gggaccactt atgaatttaa tctaattgat acccctggcc atgttgattt cagttacgag
       61 gtctcgagga gccttacagc ttgtgagggt gtacttctcg tggttgatgc ttctcaaggg
      121 gttgaagcac aaacacttgc gcacgcttat cttgcatcag actcaggact cgaactcatt
      181 cctattataa acaagattga cctaccagcc gctgacccag aaagggtgaa gaaggagata
      241 gaagaggcaa taggtataag tgccgaagag gcaatccttg caagcgcaaa agaagggatc
      301 ggaacgaagg aaatccttga agcaatagtg aagaaaatcc cgatacctct tggtagtggg
      361 gattctccac ttaaagcctt aatcttcgat agctggtttg actcatacca gggtgttgtg
      421 atgcttgtac gggtattcga aggaagaatc aaactcggta tgaagattca actcatgtca
      481 actggcaaag cctatgaagt aaagaagcta ggaatcttcg cgccaagagc attagaagta
      541 gatgagatat ctgcaggcga agttggattc gtaaccgcag caataaaaga gataagcgaa
      601 gcaaaggtag gagacacaat taccgacgct ttaaaaccta ctgacgctcc gcttactgga
      661 tttcgattga tgaaacctat ggtgtttagc ggtctttatc caatagaccc cggagaatat
      721 gataaactcc gtgacgccct tcaaaaactc cgattaaacg actctgcttt catttatgaa
      781 cccgaaacct ctcttgcact aggatttgga tttcgttgcg gattccttgg tctacttcat
      841 atggaaattg taagggaaag actagaaaga gaatttgaac ttgagcttat ttctacagcc
      901 ccaacggttg tttatatggt tacagatacg aaaggtgaag agatttacgt tgacaaccct
      961 agtaagctcc cgtcttctga gaaaatagag actatagaag aaccatacgt gctcgtaatg
     1021 ttacacacac caagcgctta tctcggagca atattcgagc tttgtgagaa caggcgagga
     1081 acccagaaga acatcaaata cataactcag gacagggtta tcgtggaata cgagcttccg
     1141 ctttccgaaa taatgtttga tttctttgac agacttaaat cggtctctcg tggatatgca
     1201 tcaatggact atgaactaat cggttataag acttctgagc ttgttaagct cgatgtactc
     1261 attaacggtg aacccgtaga tgcgctatca atcatagtcc acaaagaaaa ggcatacgaa
     1321 agaggaaagg agcttgtaga gaaactcagg aagttaattc caagacagct ttttgaggtt
     1381 gctttacaag cagcaatcgg aaatcgcgta atcgcaaaag aaaccgtaaa accagtgaga
     1441 aaagacgtca cagccaaatg ctacggcgga gacattacaa gaaagagaaa actattagaa
     1501 aagcagaaag aagggaaaaa acgtcttaag caaatcggca aaatcgaaat ccctcaagaa
     1561 gcatttctgg cagtccttaa aacctagtga tattttaggg cttgattaat caaaccgcta
     1621 actctgtgat ttgattagac ctttataaga agctgctctg tatattacct tgtgtaacgt
     1681 gaatccaaag aaaattcttt tattaacaaa aaacgcacaa tttctttata agaaactgcg
     1741 aggaatctaa tttttattcg tttaatagct taagagatcg caataaggtt tttctcgttc
     1801 ttaaaaaagg cattgggtgg tgagcttgaa tttacaaaac cctgtttaga aaccagatcg
     1861 aggtttcgtt agtttatata tagtaaaact ataattagga ggttataaaa tgactaaagt
     1921 tgtatcattt tcccttgtta cagtccttct tttttccttt gccgcctacg ggttttccgg
     1981 aaaacacgat ggtaagcatg gaaaaagtaa ttggtggaac aacactaaag tcttagaaca
     2041 actgaagctt tctgaccaac aaaaaaccaa aataggcggg atagcttcat cccacaagga
     2101 aacgctcgaa aatctccgcg ctcaaatcaa gacagcccat gaaaaactta atgaaagtgt
     2161 taaaaatccg aactcgacca aggatcgaat tttagccgag tttgatcagc tggaaaaaac
     2221 acgcggagaa ttgagaaagg ttgaatttga aatgtcactg gaaatgagag at
//
LOCUS       DATDRL010000062         5812 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2474743, whole genome shotgun sequence.
ACCESSION   DATDRL010000062 DATDRL010000000
VERSION     DATDRL010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474743"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VI935_02180"
     CDS             complement(<1..419)
                     /locus_tag="VI935_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HLE24440.1"
                     /translation="MFELSRRYIKTAFTFFIVGLFLGLYIIITKYVLGKWVSHQLITA
                     HVHVLLFGFVISLIMGVAIWMFPRPRDEMHYSPRLAEITYWFLTLGTVVRFICEITSS
                     YINSRILDWLIALGSASQVLAGLLFIYNVWSRVRPVG"
     gene            complement(633..1076)
                     /locus_tag="VI935_02185"
     CDS             complement(633..1076)
                     /locus_tag="VI935_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLE24441.1"
                     /translation="MNRSKPIFFILAFIIYLFIFNNCNSNQDEKQHDEASASINFQSF
                     QQKFKSNQDLQILDVRTLEEYKSGYIPRALVLPIQDLNSSKDVSDILSKLNKNEEVYV
                     YCSAGGPRSQRATQILRALGFKKAYFIEGGIAKWIVSGNPVTKVD"
     gene            1291..1584
                     /gene="fdxA"
                     /locus_tag="VI935_02190"
     CDS             1291..1584
                     /gene="fdxA"
                     /locus_tag="VI935_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HLE24442.1"
                     /translation="MTYVIAEPCIGVKDRACVDACPVDCIYEDEDQLYIHPDECIDCG
                     ACVEPCPVDAIFAEDELPEKWRSYAEKNRKFFEGKEGLSPAPGKSKIDERVGN"
     gene            1686..1994
                     /locus_tag="VI935_02195"
     CDS             1686..1994
                     /locus_tag="VI935_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HLE24443.1"
                     /translation="MTAITKEQIINCLKEVYDPEIGINVVDLGLIYEIEMKKGTILVK
                     MVLTSPSCPLPEVISSRVKNAIIELEGVDCVTVRIEKNRRWSPDMMTEEGRRMLDNIQ
                     "
     gene            2002..3117
                     /locus_tag="VI935_02200"
     CDS             2002..3117
                     /locus_tag="VI935_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002036164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HLE24444.1"
                     /translation="MKKPDLNSIDFNLLPFIVIWELTQACDLACIHCRAEARPWCDPN
                     ELTTEVGYRLIDEIKRFGSPLLVLTGGDPVKRKDIYDLISYSVSKGLRTTVSPSATPL
                     LTREAIRKFKESGVSRIAISLDGSYKEIHDSFRGVKGSFGWTMRAIWDTVQEKIPLQI
                     NTTVTRYNFEDLTNIASLIKNFNNVALWSVFFLVPTGRGKREDEISSEEYEAAFHIMY
                     DLSKTMPYDIKSTEAPHYRRLFIQRMVSESRNAKQPPTSNRIKVNMPDAIGRASRGVN
                     DGNGFVFISHTGEVFPSGFLPISAGNIRQESIVDIYRNSPLFRELRDYTKLKGKCGVC
                     EFRQVCGGSKARAFAVTGDYMESEPYCIYIPRNYRAN"
     gene            3473..>5812
                     /locus_tag="VI935_02205"
     CDS             3473..>5812
                     /locus_tag="VI935_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HLE24445.1"
                     /translation="MSTRKLDKIFDPKSIALVGASDTIGKLGYTLFSNLTQGGFKGPI
                     YPVNPKYKTIHGKKAYKSVTDIKGEVNLAVIVTPIDIVPEIIKGCASKKVPGAIIISA
                     GGKEVGEKGRELEEQILSEAGKGDIRIIGPNCLGIIRPSIGLNATFSHRMALPGKLAF
                     ISQSGALCTAILDKSFKENIGFSYFISIGSMADVDFGDLIDYLGSSEEVTSIILYIES
                     LTNTRKFISAARAVSRVKPIIAVKAGRSQAGAQAAASHTGAMAGEDDIYDAAFKRAGI
                     IRVRTIKELFNCAESLAKQPRPYGPRLGIVTNAGGPGVIAADTLADWGQEPANLSEET
                     IERLNLVLPPHWSRRNPVDIIGDATPERYLEAINIILQTKEIDGLLVMLTPQAVTSPT
                     DVARSISKLASKQRIPIFAVWMGGEEVEKGISILNEASIPTYDTPEEAVNTFMHMHSY
                     SYHLKLLQETPRELHTELNLDRKKAGEIIKRSLGKNSTLLPEPESKEVLSSYGIPVNR
                     TLISTSPEHAVDIAEVIGFPVVLKIHSPDIVHKTEAGGVILNLNSEEEIESAFKKIVE
                     NARNYNPKAQITGVTVQRMIKDRGYELILGSKQDPLFGPIILFGMGGIITEVIRDKAI
                     GLPPLNSTLARRLMEETKVYNLLRGFRNRPPVNLESLEEILVRLSHMVTDFPEITEID
                     INPLFVNESSILALDARVIIKSTNLRSPEHMVISPYPHRYETHWTLKDGTRVILRPIK
                     PEDEGMMIELFKTFSDTTILFRFFHILKSMSHEQIARYTQ"
BASE COUNT         1842 a         1113 c         1258 g         1599 t
ORIGIN      
        1 ttccctacag gtcttactcg tgaccatacg ttatagatga aaagaagtcc tgctaacacc
       61 tgcgaagcgc tacccaaggc gattaaccag tcaagtattc tcgaatttat ataagaacta
      121 gtaatttcac aaatgaatcg aactacagtt ccaagagtta aaaaccaata agttatctcc
      181 gcaagcctag gactatagtg catttcatct cgtggacggg gaaacatcca gatagccaca
      241 cccataataa gggatattac aaatccgaaa aggaggacat gaacatgggc ggtaataagc
      301 tgatggctta cccacttacc tagaacatat ttggttataa taatatatag accaagaaat
      361 aaaccgacta taaagaaagt aaatgcagtt tttatgtacc gtctcgatag ttcgaacatt
      421 ggatcaccac taattcttta gaaagtcttg tttttcatcc ataatatcat tgcaagttta
      481 gatttgtcct atcaaggcac cagtgaaagt gaatgtcaaa aatcggtaag agcggtttct
      541 agtcgtgact ttaaatcgca ccaagatggt gttcctacca gtcgtcgggg cacacattgt
      601 ttaagataca tcaaaacata aggaaaattt tactaatcaa cttttgtaac aggatttcct
      661 gatacaatcc atttagcaat cccaccctct atgaaataag cctttttaaa ccctaaagca
      721 cgaagtatct gagtagcacg ttgcgagcgt ggaccgccag cgctgcaata gacataaact
      781 tcttcgtttt tattgagctt tgataatata tcgcttacgt ccttagatga gtttagatcc
      841 tgaataggta aaaccagcgc tctaggaata tacccgcttt tatattcttc cagagttctt
      901 acgtctagta tctggagatc ttggttactt ttaaattttt gctggaatga ttgaaaatta
      961 atgcttgcgg aagcctcgtc gtgttgcttt tcatcttgat tgctattaca attgttgaag
     1021 ataaaaagat agattataaa cgctaatata aaaaatattg gttttgatct attcatactg
     1081 tacccaatct tataaattgt aggcaagctt tataccaggt tacttcgcta ctaatttcaa
     1141 caatatcgtc tgaaaccggt ttgtaaaagt gtcagatttt attacatctg acactttttt
     1201 taagccctgt acacctaata ttcagggaat tttcttggca cattcgttgc aaatttaact
     1261 catcgcttaa attgtaaagg aggcaaaatc atgacatacg ttattgctga accgtgtata
     1321 ggagtgaaag atagggcctg tgtagatgca tgccccgttg actgtatcta cgaggatgaa
     1381 gaccaacttt acattcatcc agacgagtgc atagattgcg gggcatgcgt agagccctgt
     1441 ccggtagatg cgatatttgc ggaggatgaa ctcccagaga aatggaggtc ttacgctgag
     1501 aagaaccgca aattctttga gggtaaagag ggcctttctc cagcccctgg aaagtctaaa
     1561 atcgatgaaa gggtagggaa ttaaaataag taaagtaaat ttagcttgag agcttagcta
     1621 taatcataga ggttcacgat caatcaaagg taaaaataat aattttttca taagtaggct
     1681 gtaacatgac cgctatcaca aaagagcaaa ttataaattg tcttaaagag gtttacgacc
     1741 ctgagattgg aataaatgtt gttgatttag gcctgatata tgaaatagag atgaagaaag
     1801 gaacaattct tgtaaaaatg gtgctaacat ccccaagttg tccattgccc gaggttatca
     1861 gctcaagagt taagaacgct attatagaat tggagggggt tgattgcgta acggtgcgga
     1921 ttgaaaagaa ccggaggtgg tcacctgata tgatgactga agaagggaga agaatgttag
     1981 ataatattca ataaaacgca tatgaaaaag cctgacttaa atagcattga ttttaacctt
     2041 ctaccattta tcgtcatctg ggaattaacc caagcatgtg accttgcctg cattcactgt
     2101 agagccgagg caagaccatg gtgtgaccca aatgaactta caactgaagt aggttatcgg
     2161 ctaatagatg agataaaaag atttggttca ccccttttgg tactgacggg aggcgatcct
     2221 gtaaagcgta aagatattta cgaccttata agctacagcg taagtaaagg actccgaact
     2281 actgtatctc ccagtgctac cccccttctt acaagggaag ccatacggaa attcaaagaa
     2341 tcaggagtgt caaggattgc tatcagcctt gatggctcct ataaagaaat ccatgattct
     2401 tttagaggag taaaaggaag ttttggatgg acaatgagag cgatatggga cactgtgcaa
     2461 gagaaaatcc cactccaaat aaacaccact gtaacaaggt ataactttga agatttaact
     2521 aacattgcgt ctctaattaa aaatttcaac aatgttgctc tatggagcgt attctttctc
     2581 gttcctactg gtagaggaaa gagagaagat gaaatttctt cagaggaata tgaagctgct
     2641 ttccatatca tgtatgacct atcaaaaacc atgccctatg atataaaatc cacagaggcc
     2701 ccacactata gaaggctatt tattcaaaga atggtatctg aatctagaaa tgctaagcaa
     2761 cctcctactt caaaccgcat aaaggtcaat atgccggatg ctattggcag agcatcaaga
     2821 ggagtaaatg acggaaatgg atttgtgttt atatcgcata ccggtgaagt cttcccaagt
     2881 ggttttctgc caatttctgc aggaaatata aggcaagaaa gtatcgtaga tatttataga
     2941 aactcacctc tttttagaga gcttagagat tacactaaac tcaagggtaa atgcggggtc
     3001 tgtgagttca ggcaggtttg tggaggctca aaagccagag cctttgccgt aacaggggac
     3061 tatatggaat ccgaacccta ctgcatttat atacctcgaa actatagggc aaactaaccc
     3121 acaatagcgg cagagtttag acctccatta attacactgt agattagggt ttcagccgcc
     3181 gacaaggtct tcaataaaat ggaaacctag aggtttctgc ctcgattgat tttaaacata
     3241 ataagaaatc ccctcctgtt gactatgtta ttcttttaca tcccagatag aactcggact
     3301 ttaagtcccg cattagcagt ggcaaaatac atcatgaaaa aatttgctca tacacaagcc
     3361 ctcaaaatgc cctcttatcg gattgaagta gaaaactggg atgggtagtc tggcgtttat
     3421 cggaaaatct agtgtgagga cggtggtctt acttttctaa ctggtaatta atatgagtac
     3481 tagaaaacta gataagattt ttgacccaaa atccattgcg ctggtagggg caagcgatac
     3541 cattgggaag cttggttaca cgctctttag taacttaacc cagggtgggt ttaaaggtcc
     3601 gatctatcca gtaaatccca agtataaaac tattcatggt aagaaagcgt ataaatcggt
     3661 taccgatatc aaaggtgaag taaaccttgc tgttattgta acaccgatag atattgttcc
     3721 tgagattata aagggttgcg caagtaaaaa agtacctggg gctattatta tctcagcagg
     3781 tggaaaagag gttggagaaa aagggagaga actggaagaa caaatcctca gtgaagcggg
     3841 taaaggggat atccgaatta tagggcctaa ctgcctgggg ataatacgcc ctagcattgg
     3901 gctaaatgca accttctctc acagaatggc tctgccaggg aaattagcct ttatttccca
     3961 aagcggagcg ctttgcactg cgatcttgga taagtctttc aaggagaata taggctttag
     4021 ttactttatc agtatcggtt ctatggctga tgtggacttt ggagacctca ttgattactt
     4081 aggcagtagt gaagaggtaa caagcattat tctttatata gagtccttga ctaacacaag
     4141 aaagtttatt agtgcagctc gggctgtgtc tcgagtaaaa cctataattg ccgtaaaggc
     4201 aggaaggagt caggccggag cgcaggctgc cgcctctcac accggcgcta tggcgggtga
     4261 ggacgatatc tacgatgcgg cttttaagcg tgctgggatt attagggttc gcactattaa
     4321 agaactcttt aactgtgccg agagcttagc caagcaaccg aggccttatg gcccacgttt
     4381 agggattgtc acaaacgctg gtggtccagg ggttatagcc gccgatacac ttgccgactg
     4441 ggggcaggag cctgctaact tgtccgaaga aacaattgag cgtcttaact tagttctacc
     4501 tcctcattgg agtagaagaa acccagttga tataattggg gatgccacac cagaacgcta
     4561 tttagaagct atcaatatca ttcttcagac gaaggaaatt gacggactac ttgtaatgtt
     4621 aacgccacag gctgtcacca gtcccacaga tgtggcaagg agtatttcca aattagcatc
     4681 caagcagcgt atacctattt ttgccgtctg gatgggtgga gaagaggttg aaaaagggat
     4741 ttctatcctg aatgaagcga gtataccgac ctacgataca cctgaggagg ctgtaaacac
     4801 atttatgcat atgcactcct acagctatca ccttaagctc cttcaagaaa ccccgaggga
     4861 gcttcatact gagcttaact tagaccgtaa gaaggcaggg gaaattataa agaggtcttt
     4921 aggtaaaaac tcaactcttc ttcctgagcc ggaatcaaag gaagtcctat caagttatgg
     4981 aatacctgta aacagaactc taatatctac atctccagag catgcagtgg atatagcaga
     5041 ggttataggg tttcctgtgg tgctaaaaat tcattcaccg gatattgttc ataaaaccga
     5101 ggcaggggga gtaattctaa acctaaattc agaagaagag atagaatcgg cttttaaaaa
     5161 gatagtagaa aacgctagaa actataatcc taaagcccag attactggtg tcaccgtgca
     5221 gcgtatgata aaagataggg gttatgagtt aatcttgggg agtaaacagg accctctatt
     5281 tggtccaata attctttttg ggatgggagg aataataacc gaggtaattc gggacaaagc
     5341 aattggtctt cctccactga actcgactct tgcaaggcgg cttatggaag agacaaaggt
     5401 ttataattta ctaagaggct ttcgtaacag accgcctgtg aatttagaat ccttagaaga
     5461 gattttagtc cgtctttcac acatggtaac cgattttccg gagattacgg agatagatat
     5521 taatccgtta ttcgtgaacg agagttccat tttggcctta gatgccaggg tgataattaa
     5581 atctacaaat ctgagatccc ctgagcacat ggtaataagc ccctatcctc atcgctatga
     5641 gactcattgg acactgaagg atggaaccag agtaatcctt cgccctatta agcctgagga
     5701 cgagggtatg atgatagagc tttttaaaac cttttcggat actacaatcc tatttaggtt
     5761 ctttcatatt ttaaaatcca tgtcacacga acagattgcc cgctatactc aa
//
LOCUS       DATDRL010000063        14891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2785677, whole genome shotgun sequence.
ACCESSION   DATDRL010000063 DATDRL010000000
VERSION     DATDRL010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14891
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2785677"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VI935_02210"
     CDS             <1..472
                     /locus_tag="VI935_02210"
                     /inference="COORDINATES: protein motif:HMM:NF015277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HLE24446.1"
                     /translation="FKPNEALFLDNTMDCNQLEKHLDTILPTKNIIYAFKIEGDFKYI
                     KTRSVSKQSKPYPPLIEVVKDQPTFEFHDAKGTIAGFWFPSYTQGINVPGYHFHFITG
                     DRKAGGHILDCQLHNGRIEIDYLSELFMALPENSDFYNVDLTKKKTGELEKVEK"
     gene            complement(538..2229)
                     /locus_tag="VI935_02215"
     CDS             complement(538..2229)
                     /locus_tag="VI935_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLE24447.1"
                     /translation="MKKFNKPVRKAPSIRPGFGVRTLPEMIEMCVEKYSDNIAYLIPR
                     KNSIYKLSYGQVLQYVKRLGRYLKELGLERGDHIAILGENRPEWAISYFSIAWIDAAA
                     IPLDARASNEAHKFILRFSTAKAIIVSGSYLSDIKSIKDELRELKHIIVMENFDEIYS
                     QYTTGVERENASLDDLLEILFTSGTTGVPKGVMLTHGNVMSNVDDIYKVIECTPDDRA
                     FSVLPIHHAYECTAGMLTQFYNGTSVFYSRSLKPNEMIEDLKTARPTVWLNVPLILEK
                     LYIRIGKELSNQNGFKGFLTNVLPKKFIGNKIKERLGLDRIKLIVSGGAALPEWVSRG
                     LEELGFPIIQGYGLSEASPLITVNPPSKPKNESVGMVIPSDEVEIRDIDSEGNGEIVA
                     KGPNIMKGYYKNESATKEALTHDEWLMTGDVGYFDEEGYLYITGRKKFVIVTKGGKNI
                     SPEEIEEKLTKSTYIEEVLVFSPDDQNIQAIIYPNLNEVEIALKTLGKVANDENIWGL
                     LRLEVKRINETLEAYKKIRHFAIRFEEFPKTTTSKIKRHLFRATNLTPGIKVFRD"
     gene            2561..3499
                     /locus_tag="VI935_02220"
     CDS             2561..3499
                     /locus_tag="VI935_02220"
                     /inference="COORDINATES: protein motif:HMM:NF019272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HLE24448.1"
                     /translation="MKRFLFLIIFLQASLVYATEEEGCGSWTSKLPMPTSRTEVAAAL
                     LDEKIYVVGGFDSQGETNLVEVYDISANTWQKGVLLPIPLHHVGAASVGGKIYVIGGY
                     TKNWEPVNTVFEYDPKQEKWTNRSPMPTKRGALAVGVVGGKIYAIGGVGAGHRENVSA
                     NEIYDPKTDKWEKRAAIPTGRDHLAIGVVSGKIYAIGGRINGNYGKNLNVNEVYNPAS
                     DKWGNNSLLPTKRSGIAAAVLEGKIYVFGGDQRAGTFNTNEKYDPITDSWEECKPMPT
                     SRHGLSAVTFENKIDVIGGGPIPGLSFSNVNEIFTP"
     gene            complement(3539..3721)
                     /locus_tag="VI935_02225"
     CDS             complement(3539..3721)
                     /locus_tag="VI935_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24449.1"
                     /translation="MVRIELSSDEVEMLHEVLSIYLSDLRMEIADTDSMNYRETLKKT
                     EVFLKDLLKRLEEKEA"
     gene            3898..4194
                     /locus_tag="VI935_02230"
     CDS             3898..4194
                     /locus_tag="VI935_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLE24450.1"
                     /translation="MADERWFEKRASHMAKGWWEFYNAVEGDTLLDKKTKALIAISAS
                     VVGRCPHCSESRIKKAIALGISKEEIAEAIMLTALLSSGTDIFWIKDTYEKYLG"
     gene            complement(4538..5455)
                     /locus_tag="VI935_02235"
     CDS             complement(4538..5455)
                     /locus_tag="VI935_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLE24451.1"
                     /translation="MIPADETFNGTFPFKPHFSEATGFRMHYVDEGKGESIICLHGEP
                     TWGYLYRKFIPPLSKTHRVIVPDHMGFGKSETPQDKEYHLRTHVENLTALIEELDLWD
                     ITFVVQDWGGPISGAYSVRHPERVKRFCLMNTLLGYSPFVKNAPKLPPLHTSPWFTWV
                     IESLKDGSYYEVMGNLGSTVLSVMKILGFENSAVVNETWLRAYSAPFENKAECKGAIE
                     FPLDVALQRVANYVKEGAGGVEELQKKPAMLAEGMRDRAIPPERAITDFKTLFPNGAI
                     VELENVGHFCQEDAPETLVALIQQFIQMT"
     gene            5672..6142
                     /locus_tag="VI935_02240"
     CDS             5672..6142
                     /locus_tag="VI935_02240"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HLE24452.1"
                     /translation="MDQLAFQDHMERNKCWGCGSSNEYGLHIKSYWSGDESVCTWKPH
                     QHHMAGPTHILNGGIIATLIDCHSVCTAIAAAYREEGRELNTEPFIWYVTASLKIDYI
                     RPTPISEPVTLRARVKEMRGRKTIVTCSLLSNGEECARGEVIAIRVSPDWYEEK"
     gene            6155..6562
                     /locus_tag="VI935_02245"
     CDS             6155..6562
                     /locus_tag="VI935_02245"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HLE24453.1"
                     /translation="MERNIQNIGLLFIRILAGGTMLVAHGVSKLFDPKPFIEALSSNG
                     FPLPNILAYLSISAEAVFPLFIILGVFTRISALIAAINMFVAAFVFHLNIKGDPFANF
                     EKAFLYMIVFIFIAIAGGGDWTLKSYFDRSRRT"
     gene            complement(6714..8114)
                     /locus_tag="VI935_02250"
     CDS             complement(6714..8114)
                     /locus_tag="VI935_02250"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HLE24454.1"
                     /translation="MASGDVREKGAILWTRVNQEAEVLAEVSTDCSFTQVDKMEMGKA
                     LAESDFIIKVTASDLEPDHTYCYRFKTEAAVSDVGTFRTAPSEGQDTTVRFAYSSDSD
                     GSKNPPFNNFEVLDLVRLDDIDFWVYLGDTVYMDTFGPKAETLDEIRAKYKQNRDFVA
                     LRDLLKATSTYAIWDDHEVENDFDGQTVAPTLYANGRRAFLEYMPIQESGLLNDPTCA
                     GNPLFRVFHWGKNVDIIILDERSCRSASVEEACEDDLIPTLPTEIRLAFGLLPQPPPG
                     CLDAINDPARTMLGINQKEALKNALLESKATWKLIINEVAISEFFALPYDHWEGYAAE
                     REELLNFIISNNIKNVVFLTGDLHANLIGDVVLNRFMAPIPIANEIIAGPIAQVTLFE
                     RLEDQLGSTDAVSALISLATLANLQCVHTNTFGYALVEANSDSLTITLKDDNGQQIQD
                     QLNRPGASCTITIQAQ"
     gene            8946..9221
                     /locus_tag="VI935_02255"
     CDS             8946..9221
                     /locus_tag="VI935_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HLE24455.1"
                     /translation="MARIVKREGPLVVALRTEGAVIIPKVLREKLGLRAGDLLEIEVR
                     GGDLVLHPIPIRRLMLKGVKAAFLDKLTGLVRLGGDALKDKKRLYER"
     gene            9211..9663
                     /locus_tag="VI935_02260"
     CDS             9211..9663
                     /locus_tag="VI935_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLE24456.1"
                     /translation="MSVRVLVDTSFLVAQLDERDVHHQTAKALHELFRKREVAYIYLD
                     CVMNETVTVLARRALERKADQRAIIRRLRKEIPTDLLDWEGPELPRLWERALDTLEMY
                     KGRLSFNDCLLVLICQEGGIEWIASFDQSFDKVSGIKRVGKTAAFTTR"
     gene            9890..10204
                     /locus_tag="VI935_02265"
     CDS             9890..10204
                     /locus_tag="VI935_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HLE24457.1"
                     /translation="MKKRQSKAFMGIPREKVVEFCKRNHIHKFALFGSVLRKGFHPNS
                     DLDVLVEFEPGHVPGLAFFSMEAELSDMLGRKVDLNTPNFLSPYFRNEVLAEAEVFYV
                     KE"
     gene            10283..10540
                     /locus_tag="VI935_02270"
     CDS             10283..10540
                     /locus_tag="VI935_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HLE24458.1"
                     /translation="MDNDCLLNLALVRLLEIIGEAANQTSKEERSRYPEIPWSQIVSL
                     RNRLIHGYDAVDFDILWQILAQDLPLLITALEKIVPPEETL"
     gene            11030..11842
                     /locus_tag="VI935_02275"
     CDS             11030..11842
                     /locus_tag="VI935_02275"
                     /inference="COORDINATES: protein motif:HMM:NF025881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HLE24459.1"
                     /translation="MKKYGISRRDKATAREFAQTKGKVVFNREDELGNKTKVTLQKNI
                     INKDFFKSWSPAMAYVLGVLYTEGNLHPGILRDPTAKGTSAASRFTVSQKEPELLQKI
                     LVLMGSNAKLLFRKKKGISGAVYYFHINDNEIYEDLLRLGLSPNKSLSLVFPNMEPLY
                     VKHFIRGCWDGDGSVYWENNDPVKPRASFISGSKIFAEGILKLLVGLGLSDRRMYTTK
                     SGRAFYFRFLGQDCAKLYHIFYDGVPESMHLTRKYERFKAIGNYFETDVRMC"
     gene            11925..13202
                     /locus_tag="VI935_02280"
     CDS             11925..13202
                     /locus_tag="VI935_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24460.1"
                     /translation="MKLKLKIPLYFYLSGPTEQSLEVETSFEHDDVKVNLRFVGENPL
                     TFPKGSDDSKVFSHVKYAEFVLEDTDPQQSRLAKIVTTEPDYVQILKYLLPIVNRVLC
                     SIRNWGLVAFVAELNPRDDDAKKYIQRWDVQSSEDGENWQAVVKPETGLLSVLLSSFF
                     LNDEKAGNLHVKDWSDIEEAIQDNLEPPPEQEFLTNSIEHLNLGNFRLALVEAIICLE
                     IVLTQFLQVFLSIDKRISKSRINDFLSPQFGLKAKVSALLDLILDPNDLKKINVDNVR
                     TAVEWRNGVIHKTGHLPQGVPEEELRRKINDVLSLALLLGRKREQISSSPELQRIGNQ
                     LSQKFRLPNPTILGLGRHRILLDFDFLLSSEIPQDLEEVLKSVADETSSIFTTKDSRF
                     NAAEHLHIRFLVLFPFPQKVKARWRNNKIEVVE"
     gene            13325..14041
                     /gene="ccmA"
                     /locus_tag="VI935_02285"
     CDS             13325..14041
                     /gene="ccmA"
                     /locus_tag="VI935_02285"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HLE24461.1"
                     /translation="MESSDLDIVAESVTKSFGYIQALRGIDLKIKKGEFVTVFGPNGA
                     GKTTLIKLLATLTKPTSGKVSISNHDIKKEPDKVRALIGVISHDPYLYGNLSALENIG
                     FFASLYGISQPKEKAIQVIKQVGLESRMYDLVRTFSRGMQQRLAVARAIVHEPKILLL
                     DEPYTGLDQHGARIFGELLNWLKTENRTIVMTTHNLPEGLEISDRVAILDGGRIIYES
                     DARGIELTKFKEIYFEKVGN"
     gene            14070..14435
                     /locus_tag="VI935_02290"
     CDS             14070..14435
                     /locus_tag="VI935_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HLE24462.1"
                     /translation="MKDELTEKIIGAAIEVHRILGSGLLESIYEEALCHELTLQNINN
                     ERQVAVDVFYKGHVIQGQRLDLLVEGEVIVEIKSLKELPDVAMAQVLSYLKATGLKRV
                     LLINFGEKRLIDGIKRISL"
     gene            14460..>14891
                     /locus_tag="VI935_02295"
     CDS             14460..>14891
                     /locus_tag="VI935_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HLE24463.1"
                     /translation="MKKIWAIVWKDIITELRTKELFASMFTFALLIIVIFNFAFDFSA
                     EIIPFAAPAILWISFTFAGVLGLSRSFALEKEGNSILGLLLTPTDRSLIFLGKMIGNI
                     IFVFIVEAIILPIFVLFFNYNLFGNAVPLLLVILLGTIGFVS"
BASE COUNT         4345 a         2814 c         3242 g         4490 t
ORIGIN      
        1 cttcaagccc aatgaggcac tatttttaga taatactatg gattgtaatc agttggaaaa
       61 gcatttagat actattttgc caacgaaaaa cattatctat gcctttaaga tagaaggaga
      121 tttcaagtac ataaaaacta gaagcgtttc gaagcaaagt aagccatatc cgcccctaat
      181 tgaagtcgta aaagaccaac caacattcga gtttcatgat gcaaaaggga caatagcagg
      241 attttggttc cctagctaca cgcagggtat aaatgttcca ggctatcatt tccattttat
      301 tacaggagat aggaaagctg gagggcatat actcgattgc cagttgcata atgggagaat
      361 tgaaattgat tatctttctg aattatttat ggcgttgcca gagaatagtg atttttataa
      421 cgtggattta acaaaaaaga aaacgggaga actcgagaag gtagaaaaat aacttggatt
      481 taaaaactcc tttatagttt cagtggagat tttattataa gagtcctaga ggcagtatta
      541 atctctaaac acctttattc cgggtgttaa atttgtagct ctaaacagat gtcttttaat
      601 cttactcgtc gtggtttttg ggaactcttc gaatcttatc gcaaagtgcc taatcttttt
      661 ataagcttca agggtttcat taatccgctt aacctctaat cttaaaagcc cccatatatt
      721 ttcatcatta gctactttgc ccaaggtttt taatgcgatt tcaacctcat taaggttggg
      781 gtagattata gcctgaatgt tctgatcatc tgggctaaat acaaggacct cttctatgta
      841 tgtggattta gttaattttt cctcaatctc ttcaggagat atattctttc cacctttagt
      901 aactattacg aatttttttc ttccagttat atatagataa ccctcttcat caaagtatcc
      961 aacgtcccct gtcattagcc attcatcatg ggttaaagct tcttttgtag cgctttcatt
     1021 cttataatac cccttcataa tgtttggacc cttggcgact atttcaccat tgccttcgct
     1081 gtctatatcc cttatttcaa cctcatcgct cggtattacc ataccaacac tttcattctt
     1141 aggttttgat ggggggttta cagtaatcag tggggatgcc tccgaaaggc catacccctg
     1201 tattattgga aatccgagtt cttccaagcc ccttgaaacc cactcgggta gagctgctcc
     1261 accagatact atgagcttta ttctatcaag ccccaatctt tccttaatct tattacctat
     1321 gaacttctta ggtaaaacat tagttagaaa tcctttaaat ccattctggt tggataattc
     1381 tttacctatt cttatataaa gcttttccaa aatgagtgga acattgagcc atactgttgg
     1441 acgcgcagtc tttaaatcct ctatcatctc attgggcttt aaacttctag agtagaatac
     1501 acttgtgcca ttataaaatt gggttagcat tcccgcggta cattcatatg catgatgaat
     1561 tgggagaact gagaatgctc tatcatcggg tgtgcactct atgaccttat agatgtcatc
     1621 tacgttagac ataacgttcc catgggttag cattacaccc tttgggacac ctgtagtacc
     1681 tgaggtaaaa agaatttcta aaaggtcatc caaagaagca ttttcccttt caaccccagt
     1741 cgtgtattga ctataaattt catcaaaatt ctccataaca attatatgtt ttagttcacg
     1801 caattcatcc tttatagact taatatcgga tagataagag ccggatacga ttattgcttt
     1861 tgctgttgag aatcgaagaa tgaacttgtg tgcttcattt gatgccctgg cgtcaagtgg
     1921 tatagctgca gcatcaatcc aagcaatcga gaagtaggat attgcccact ctggtctatt
     1981 ctcaccaagg attgctatat ggtctcccct ttcaagcccg agttctttca gatacctacc
     2041 taggcgtttg acgtattgaa gaacctgacc gtagctgagc ttatagatac tattcttcct
     2101 aggtataagg taagcaatgt tatcgctata tttctctaca cacatttcta tcatctctgg
     2161 aagggttcta acaccaaatc ctggtctaat tgatggcgct tttcgcacag gtttgttaaa
     2221 ctttttcata ttttaatttt aaaaatttca tttgctgttt tattatatta ttgcatgtaa
     2281 tttattaaat gggtaatggt aaactaacag ggtaggcaat gaattccctt agctaagctc
     2341 ggggcaagct tgctcgttgc cgtccaccag caagccttcc ccgactggat cggggaccga
     2401 gctcaggcga aacgactggt ggggtaccct agtgtttatt atttgattta attttcaaag
     2461 aggtactata ttataacctt tgaattactg gtaaagtcat ttctctacct accccttgga
     2521 acgaaacaag atggttaaaa ttttgccggt ctaaaagcat atgaagaggt ttttatttct
     2581 aattatattc cttcaagctt ctttggtata cgctactgaa gaggagggtt gtggaagttg
     2641 gacttctaaa ttgcccatgc ctacatcgcg cacagaagtg gcggcggctt tacttgacga
     2701 aaagatttat gtagttggag gttttgattc tcaaggtgaa acaaatttag tggaagtata
     2761 cgatatttct gcaaacacct ggcagaaagg cgttttattg cctatccctc ttcatcatgt
     2821 aggtgcggct tcagttggag gaaaaatcta cgtaataggt ggttacacaa aaaactggga
     2881 gcctgtaaac accgtgtttg aatatgatcc caaacaagag aaatggacta ataggtcacc
     2941 tatgccaaca aaaaggggag cgcttgctgt cggggtagta ggggggaaaa tctatgcgat
     3001 aggtggggta ggagcaggtc atagagaaaa tgtctccgca aatgagattt atgaccctaa
     3061 aacggacaag tgggagaaaa gagctgcaat tcccactgga agagatcacc ttgctattgg
     3121 tgttgttagt ggaaagattt atgcaatagg tggaagaatc aacggaaatt acggaaagaa
     3181 tctaaatgtt aacgaagtgt ataatcctgc ttcggataag tggggaaata atagcctgct
     3241 tcctacaaaa aggagcggta tagcggctgc agtactcgaa gggaaaattt atgtctttgg
     3301 tggggaccag cgtgctggta catttaatac taatgaaaaa tatgatccta tcacagactc
     3361 atgggaagag tgcaaaccta tgcctacatc cagacacggc ctttcagcgg ttacttttga
     3421 aaacaaaatc gatgtgattg gaggaggtcc gataccgggg ctgtcattta gtaatgtaaa
     3481 tgaaattttt acgccataaa gaaacagttc tttcgaattt caggtaaggg taatgtcttt
     3541 aagcctcctt ttcctccaga cgtttaagga gatcctttag aaacacctct gtcttcttaa
     3601 gggtttcgcg gtagttcatg ctatctgtat ccgcgatttc catacggagg tcagaaagat
     3661 agattgagag aacctcatgc aacatttcaa cttcatcaga actcaactca attcgtacca
     3721 tatgttccct cccttcaagc ttcggtttgc ttctgataag tttaactcaa agtttcaatt
     3781 aataacactt atacttttaa ttgagaaaga tctttgaatc cctatattca atacctcatc
     3841 ttaatatgtt aggatttgtt ctaggattaa taaaaaacat taaggagggt ttagaaaatg
     3901 gcggatgaaa ggtggtttga gaaaagggct tctcatatgg ctaagggctg gtgggaattc
     3961 tataatgcag ttgaggggga tactctttta gataaaaaga caaaggctct aatcgcaatt
     4021 tctgcttctg tagttggtag gtgcccacac tgctcggaat cacgcataaa gaaagctata
     4081 gctttaggta taagcaaaga agagatcgcg gaagcaatta tgctgactgc attgctttcc
     4141 tctggaacag acattttctg gataaaggat acttatgaaa agtatctggg ataattgatt
     4201 gtctttcaaa tattaatcta gtaataaata acctaactgg gtaggcaacg agtcttgctc
     4261 gttgccacct agctcaccag caagcctgtc ctgagtttat cgaaggaact agtaggttat
     4321 cctatgttgg tttttattac ctaattagtt tttcaaaaac aataagccat cctgatcgct
     4381 ttattattca tcagacttta tttgatagtt tttatgagtt tcttttacct tgagggcaga
     4441 ttgtaccgag aatcagctag ggtgcatctt gactaataaa gtgagctggg caggtaattc
     4501 atgttccatt tctgaagagc cttaacaggg attttcccta tgtcatctga ataaactgct
     4561 gaataagtgc gaccagggtt tctggtgcat cttcctggca gaagtgaccg acgttctcta
     4621 attcgactat tgccccgttg gggaaaaggg ttttgaagtc agtgatagcc ctttctggag
     4681 ggatcgcccg atcgcgcatg ccttcggcaa gcatggctgg tttcttctgt aattcttcaa
     4741 ctccgcctgc tccttccttg acataattgg ctaccctttg taaggccaca tcgagaggga
     4801 attcgattgc acccttacat tccgctttat tttcgaaagg cgcagagtag gcgcggagcc
     4861 acgtttcatt gacaaccgct gaattctcaa aacctaagat tttcattacg ctgaggactg
     4921 tacttcccag attacccatc acctcgtaat aagaaccgtc cttgaggctt tcaataaccc
     4981 atgtaaacca gggtgaggtg tgtaagggtg gcaacttagg tgcatttttt acaaagggac
     5041 tataaccaag aagcgtattc attaagcaaa agcgtttgac gcgttcaggg tgacgaacgc
     5101 tgtatgcacc acttatgggc ccgccccagt cttggacaac aaacgtaata tcccacagat
     5161 ccagctcttc tataagcgcc gtcaagtttt ctacgtgagt gcgaagatgg tattctttat
     5221 cttgcggtgt ttcgcttttt ccaaaaccca tgtgatcagg cacgatcact cgatgtgtct
     5281 ttgaaagcgg ggggataaat ttgcgataca aatatcccca tgtcggctca ccgtgaagac
     5341 aaataataga ttctcctttt ccttcatcaa cgtagtgcat acgaaagcca gtagcctcac
     5401 taaaatgggg cttaaatgga aacgttccgt taaaagtttc atcggcgggt atcatattgg
     5461 taatccttag tcgattttaa taagtcctta agcaataacc tttattcaca aaattttgct
     5521 gttaaccatt atctcactaa gaataatagg ttgtcaagaa aaaaattcta tctactttcc
     5581 atttttgtaa gaatcaggaa ttattagagg ttgtttgtat gtaaattcaa atacttgact
     5641 aagattaata atcagacctg gagtttcatt tatggatcaa cttgcatttc aggaccatat
     5701 ggagagaaac aaatgctggg gttgtgggag tagcaatgaa tatggtctgc acataaagag
     5761 ttattggtct ggtgacgaat ctgtgtgcac atggaagccg catcaacatc atatggctgg
     5821 cccgacgcat atactgaacg gaggaattat tgcaaccctt atagattgcc attcggtttg
     5881 caccgccatt gccgccgcat atagggagga gggtagggag ctaaatacag agccattcat
     5941 ctggtatgtt actgcatctt taaagataga ctatatcaga cctacgccga ttagcgagcc
     6001 tgttaccctt agggcacgcg ttaaggaaat gaggggtaga aaaacaattg ttacctgttc
     6061 ccttctttca aatggcgaag agtgcgcccg cggagaagtt atagcaatac gcgtgtctcc
     6121 cgattggtac gaagaaaaat aaggaggtta ttagatggaa agaaatattc agaatatcgg
     6181 tttattgttt ataaggatac ttgcaggtgg gactatgctt gtagcgcatg gtgtgtcaaa
     6241 gctatttgat ccaaagccat ttatagaagc tttaagttct aatgggtttc ctctaccaaa
     6301 tattttggct tacttgtcaa tttcagccga ggcggttttt cccttattca ttatcttagg
     6361 agtattcact agaatttcag cgctcatagc tgctataaat atgttcgtag cggcgtttgt
     6421 ttttcactta aacataaaag gtgatccttt tgcaaatttt gaaaaggctt ttctgtatat
     6481 gatagtgttc atttttattg caatagcagg cggcggggat tggacattga agagctattt
     6541 tgatagaagc aggaggacat aataactctg tgttatagtt tcaacaaata gggagagttt
     6601 gagccctgta tcgacccacc cggctgtgcg tttgatgccc taccgtgtag ataaatttta
     6661 aataaccaag tttaatgaac tcagcgataa aaaattaact cgatgatgca agtctattgg
     6721 gcctgtattg taatagtgca ggaagcaccc ggccggttaa gctggtcttg gatttgctga
     6781 ccgttgtcat cttttagggt gatggtcaag gagtcactat ttgcctcgac caaagcgtat
     6841 ccaaaggtgt tggtgtgcac gcactggaga ttggcaaggg tagccaaact tatgagggct
     6901 gagactgcat ctgtcgaacc cagttgatcc tctaacctct caaagagggt aacctgagca
     6961 ataggtccag cgatgatctc gttggcgatg ggtatcggtg ccataaacct atttagcacg
     7021 acatcgccga ttagattggc gtggaggtcg ccggtaagga agactacatt cttaatgttg
     7081 ttactgatga tgaagttgag aagctcctct cgttcggcag cgtagccctc ccagtggtca
     7141 taggggaggg cgaagaactc gctgatggca acttcattga tgataagctt ccaagtggct
     7201 ttcgactcta gaagggcatt cttgagagcc tccttttggt tgatacccag catcgttcga
     7261 gccggatcat tgatggcatc cagacagccg ggtggaggct gtggtagtag gccgaaggct
     7321 aaacgaatct cggtaggaag agtgggaatc aaatcatcct cacaagcttc ctccacgctg
     7381 gcgcttcggc aggagcgctc atctaagata ataatgtcca cgttcttccc ccagtggaac
     7441 acacggaaca gggggttgcc agcgcaggtt ggatcattga gcaaacccga ctcctgaatg
     7501 ggcatatatt ctaagaaagc cctacggccg ttagcataca acgtcggggc tacggtctga
     7561 ccatcgaagt cgttctccac ctcatggtca tcccagatgg cgtaggttga tgtagccttg
     7621 agaagatcgc ggagagctac aaagtctcgg ttttgcttat acttggcccg aatctcgtcc
     7681 aaggtttcag cttttggacc gaatgtatcc atgtagacgg tgtcacctag gtaaacccag
     7741 aagtcgatgt cgtccagccg aaccaggtct aggacttcga agttgttaaa tggtgggttt
     7801 ttagagccat cggagtcact ggagtaagca aagcgtacag tggtatcctg tccctcgcta
     7861 ggtgcagttc ggaaggttcc gacatcgctt acagcggcct ctgtcttgaa gcgataacag
     7921 tacgtgtgat caggttcgag atcgctagca gtaaccttga taatgaagtc gctttcagcc
     7981 aaagcctttc ccatctccat cttgtcaacc tgtgtgaaag agcagtcggt tgacacctca
     8041 gcaagaacct cggcctcctg attgacacga gtccagagaa tagctccttt ttctctgaca
     8101 tcgccactag caataccttg ggtaaagaag ctggaggatc caggacctcc tccaccctca
     8161 tcacagctaa atagagtgag ttacagtact gagattaaaa caactaagag cacataactt
     8221 agcttcttat acatatttca ccaccccctg tggtctttct aagaagcctt aacaaaggga
     8281 atataaaggt tctcttagaa ggtgaaattg aaaaatggtt tgtaggagca cacctcatta
     8341 attgcttttg gattgtgtag taacggttag tgaggtcctt cttacccatc ttaaccctgt
     8401 tcgctgcttt attaattatg attctagtat atttcgggta taagaatcaa ggaaattttt
     8461 tttggagttt aaccaggttc ttgtagctat ttattatcac gagatttctc tcgcattagc
     8521 tctgcctctc aatggcaaat gggtaagcta tggctgttga tatgaagacg tgaccttact
     8581 cagaaaccat gctataaagc ttaagttgcc tctgtcaaga acttataaaa atttgggtaa
     8641 agtccatctc gacatacgat taacaacaac gcttatatga gttagaatta acgaatggta
     8701 tttatatgtt atgataagaa cttgaaaaga ttcgggtggc aatacaattt taatgatata
     8761 gccttctgag gttacttctc ttattcttcg gaaaatcgtc tttgattact ttttcttcct
     8821 ttaggattac aatattcaca attgagaagc aaagcatctt tacatatgtt catccgtggc
     8881 tactttttct tttttcttct ttgacaaggt aacaagattc ttgtatcatt gctatagagg
     8941 taatcatggc aaggattgtt aagcgggaag gtcccttggt tgtagccctt cggacggagg
     9001 gagctgtgat tattcctaag gtccttcggg aaaagctcgg tcttcgggct ggcgatcttt
     9061 tggaaataga ggtacgcgga ggggatctag ttcttcatcc tattccgata aggcgactga
     9121 tgctaaaggg ggttaaggcg gcctttttag ataagctcac cggattagtc cgtctgggcg
     9181 gcgatgcgtt aaaggataaa aaacggctct atgagcgtta gggtcctagt agatacgagc
     9241 ttcttggttg cacaactgga tgagcgagac gtgcatcacc aaactgccaa ggctcttcat
     9301 gagcttttca ggaaacgaga agttgcatat atctatttgg actgtgtgat gaacgaaacc
     9361 gtgacggtgc tggcccgacg ggcgcttgag cgaaaagccg atcaaagggc aatcatcagg
     9421 cggctgcgta aagagattcc taccgattta ttggattggg aaggtcctga gttacccagg
     9481 ctatgggagc gggctcttga tacattagag atgtataagg gcaggttatc atttaacgat
     9541 tgccttcttg ttcttatctg tcaggaagga ggcattgagt ggatagccag ctttgaccag
     9601 agctttgata aggtcagtgg tatcaaacgg gtcgggaaaa ccgctgcttt tactaccaga
     9661 taataatttc ccaaggtctc atacctaaat atttgctttt aagtaaaccg attctcttac
     9721 gtctaactta cgttaatttt aaatccgctt attaaaagta gcaccggcct taaataactc
     9781 ttgccctttc tcccgtgttg acaagggttc attcctgatg aaataatagt gctaagagag
     9841 gttggtgtgt agatagacaa gtaggtgaaa aaaataaaca gtgggaaaga tgaagaaaag
     9901 gcaaagtaaa gcattcatgg ggattcctcg tgaaaaagta gttgaattct gtaaacgaaa
     9961 ccacattcac aaatttgcgc tattcggctc tgtcttgcgt aaaggcttcc atcccaacag
    10021 cgatttagat gttttagtgg agttcgagcc agggcatgtg ccagggcttg cgttcttctc
    10081 tatggaggct gagctttctg atatgcttgg aagaaaagtg gatttaaaca caccaaattt
    10141 tcttagtcca tatttccgta atgaagtctt agcggaagca gaagtatttt atgtcaagga
    10201 ataaaactac agttcgcctg cgtcataagt tggccatgtc cgtgaagcgg tttctttaac
    10261 acagggcaaa tccagagtag atattgacaa cgactgtctt ctcaatcttg cactggtgcg
    10321 tctgctggaa atcattggcg aggcggcaaa ccaaacttct aaagaagagc gttcccggta
    10381 ccccgaaatc ccatggtcgc agatcgtgag tctacgcaac cgcctgattc atggctacga
    10441 tgcggttgac tttgacattc tctggcagat tttagcacag gatttacctc tcctgattac
    10501 tgcactggaa aagattgttc ctccagaaga aacgttatga agtggattga ctgacttatc
    10561 gttaattacc aataccagga aacaaagcat cttcacggga gaaaagtcct atgatggatg
    10621 tttatgcgtg aataccttgt taccttagga ctgtttacag ggaatcattc tttgtgtgat
    10681 aattgtgata agagagaagt tcacgagtta tgttcgatta ctaacaattt agacgaaatt
    10741 caattcatcc aataggaata gtaatatggc ttggtcgaaa aagtatgata ggtgtttgag
    10801 atgtggaact caaacgtata aacacaaagg aagggggcta tgtgtttatt gttatcaatc
    10861 tgaaattgag aatagccata agtcacatat aacgggaggg ttgaaggaaa ggaagatatt
    10921 tgcacaaatt tctaaaaatg aattagaatt gcaatataaa gcactagaaa tgtccctcag
    10981 tgacttagct cgtaattata attgtacccg ccaatatatt cataagataa tgaaaaaata
    11041 cggaattagc aggcgagaca aagcaaccgc cagagagttc gcgcagacca agggaaaagt
    11101 tgtatttaat cgtgaagacg aattaggaaa taagacaaag gttactttac agaagaatat
    11161 tataaacaag gactttttta agtcatggtc tcctgctatg gcctatgttt taggtgtctt
    11221 atataccgaa gggaatttac acccaggcat attaagagat cctactgcaa aaggtacatc
    11281 tgcagcgtca cgatttactg tctctcagaa agaaccagaa cttcttcaga agattttggt
    11341 cttgatgggg agtaatgcta agctgctatt tagaaagaaa aaggggattt ctggtgctgt
    11401 gtattatttc catattaatg ataatgagat ttatgaagat ttattaagat tagggctctc
    11461 gcctaataaa agcctctcgt tggtttttcc aaatatggaa cctctttatg tgaaacattt
    11521 catacgagga tgctgggatg gggatggctc tgtctattgg gagaataacg accctgtcaa
    11581 accgagggca agttttatta gcggttcgaa aatattcgca gagggaattc taaaactgct
    11641 agtaggtctc ggattgtctg atcgaagaat gtatacaacg aaatcaggaa gagccttcta
    11701 ttttcgtttt ctaggtcagg attgtgctaa gctctatcac attttttacg atggagttcc
    11761 tgaaagcatg caccttacaa gaaagtacga acgttttaag gctattggta attactttga
    11821 aacagatgta aggatgtgtt agaaataaag caaaataagg aaataaaatg agaaaaaagg
    11881 acaaataaga actgttcttc attttggtta ccttatttag ttaaatgaaa ctcaaactga
    11941 aaataccttt atatttttat ctctctggac ctacagagca atccttagaa gtggaaacat
    12001 ctttcgagca tgatgatgtt aaagttaacc ttcggtttgt cggtgaaaat cctctgactt
    12061 tccccaaggg ttcagatgac agtaaggtct ttagtcacgt aaaatatgct gaatttgtgc
    12121 ttgaagatac tgacccgcag caatcaaggt tggcaaaaat tgtaacaact gaaccagact
    12181 atgtccaaat attaaaatac ctcttgccaa ttgtgaacag agtgctttgt tctatacgca
    12241 attggggcct tgttgcattt gttgcagaac ttaacccaag agatgatgat gcaaagaaat
    12301 atatacaaag atgggatgtg cagtcttccg aagatggtga aaattggcaa gcagttgtga
    12361 aacctgaaac aggtttgtta agtgttttgt tgtcaagttt tttcttaaat gatgagaagg
    12421 ctggaaatct acatgtcaaa gattggtccg atattgaaga agcaattcag gacaatcttg
    12481 aacctccacc tgagcaagag ttcttaacca attccatcga gcacttgaac ttagggaact
    12541 ttagactcgc tttggttgaa gccattattt gcctagagat tgttctcaca caattcctac
    12601 aagtttttct ttctatagat aaaagaatct ccaaaagtag aataaatgat tttctttccc
    12661 ctcaatttgg tctcaaggca aaagtatcag ctttactcga tcttattcta gatcctaatg
    12721 acttaaaaaa gatcaacgtt gacaatgtcc gaacagcagt cgagtggcgc aatggagtga
    12781 tacacaaaac tggtcacctg ccacaaggtg tcccagagga agaacttcga agaaagatta
    12841 atgatgtcct aagcctagct ctgttactgg gcagaaagcg agagcaaata tcaagttcac
    12901 ccgagttgca acggattggg aatcagttat ctcagaagtt taggcttcca aatccaacta
    12961 ttttaggttt gggaaggcac cgcattctct tagattttga tttcttgttg tcgagtgaga
    13021 taccacaaga tttagaggag gttttgaaat ccgttgcgga tgagacctct tctattttta
    13081 ctacaaagga ctcgagattc aatgctgctg agcaccttca cattagattt ttagtattat
    13141 ttcctttccc tcaaaaagtt aaggcccgtt ggcgtaacaa taaaattgaa gttgtcgaat
    13201 aatcaataaa gatactcctc cctaagcata ggtttttttc gtttttacaa aattgcaacc
    13261 aaattccttt taaggtagtt ttcttccaaa tttaatattt tactatccaa ggacaaaatc
    13321 ttaagtggaa agtagcgatt tagatattgt tgccgaaagt gtgacaaaat cttttggata
    13381 cattcaggct ctaaggggaa tagatttaaa gataaagaag ggggaatttg ttactgtttt
    13441 tggtccaaac ggggcgggga aaacaactct tataaaactt cttgctacac ttaccaaacc
    13501 tacttcagga aaggtatcca tatctaacca tgatataaaa aaagaaccgg ataaagtgag
    13561 ggctctaatt ggtgtaatct cacacgaccc ttatttgtat gggaacttga gtgccttaga
    13621 gaacatcgga ttctttgcct cgttgtatgg gatttcacaa cccaaagaga aagccataca
    13681 ggtaattaaa caggtaggtc ttgaaagcag aatgtacgac ctggtaagaa cgttttctcg
    13741 aggaatgcag cagcgtcttg ctgtagcaag agcgattgtt catgagccta agatcctact
    13801 tctcgacgaa ccctatacgg gtttagacca acacggtgca aggatttttg gagagttgct
    13861 aaattggctt aagactgaga atcgaacgat agttatgaca acccataacc ttccagaggg
    13921 acttgagatt agtgataggg tagcaatttt agacggaggt agaatcattt atgagagtga
    13981 tgcgagggga attgagctta ctaaatttaa ggagatttat tttgagaaag tagggaatta
    14041 gccacagagt acacagagaa aaagagaaaa tgaaagatga attgacggaa aaaatcatag
    14101 gtgccgccat tgaagtccat cgcattttgg gatcaggact tttagaatcc atttatgaag
    14161 aagcactttg tcacgaactc acacttcaaa acatcaacaa tgaaagacaa gttgcagtag
    14221 atgtgtttta taaaggtcat gttattcagg gccagagact agatttactt gttgaaggtg
    14281 aggtgatagt tgagataaaa tccttgaaag aattaccaga tgtggctatg gctcaagtgc
    14341 tctcatatct taaggcaact ggtttaaaac gtgtcttact tataaacttt ggggaaaaga
    14401 gacttataga tggtattaaa agaatctctc tttaatctct gtgctctctg tggctaaaca
    14461 tgaaaaaaat ttgggcgatt gtttggaagg acataattac tgaacttaga acaaaagagc
    14521 tttttgcgtc tatgtttacc ttcgcccttt taattatagt tatttttaat tttgcttttg
    14581 atttttcggc tgagattata ccttttgcag ctcctgctat tctttggatc tcttttactt
    14641 ttgctggagt gttgggtctt agccgttcat tcgctcttga aaaggaggga aattcgattc
    14701 taggtctact tcttacacca acggatagaa gcctaatttt ccttgggaaa atgattggaa
    14761 acataatatt tgtttttatt gtcgaggcaa ttattcttcc tatatttgtt cttttcttta
    14821 attacaattt gtttggtaat gctgttccac ttttacttgt gattcttctt ggtactatag
    14881 gtttcgtttc a
//
LOCUS       DATDRL010000064         2889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1942989, whole genome shotgun sequence.
ACCESSION   DATDRL010000064 DATDRL010000000
VERSION     DATDRL010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942989"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            637..1059
                     /locus_tag="VI935_02300"
     CDS             637..1059
                     /locus_tag="VI935_02300"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HLE24464.1"
                     /translation="MGYKTLLTKRVEFSASHRYWNPKWNEEANKAVFGKCISPYGHGH
                     NYVLEVTVEGTVDTDTGMIINIYDLKPLINEVLKDFDHKFLNEDNPYFRDLIPTTENI
                     ARVLLGLIEKKLKETTDCKLYRIRLYETPDLFVDYWRE"
     gene            1059..1496
                     /locus_tag="VI935_02305"
     CDS             1059..1496
                     /locus_tag="VI935_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011230966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HLE24465.1"
                     /translation="MRREICTLTKIYHFSAAHRLHATRYSDEENRRIFGKCNNPKGHG
                     HDYHIEVKVTGNIDPETGMVINLSELDEMMKDIVEELDHTRLDIEVPHFREFVPSGEN
                     IAKYIWMRLQPKMKKGAPLHIKLWETQDNYFEYFEEDNYSANE"
     gene            1615..2169
                     /gene="folE"
                     /locus_tag="VI935_02310"
     CDS             1615..2169
                     /gene="folE"
                     /locus_tag="VI935_02310"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HLE24466.1"
                     /translation="MSIEKAIKEILLEIGEDPERQGLKNTPDRIAKMYKELTVGYRVS
                     PQEIINGAIFDIKYDEMVIVKDIEFYSLCEHHLLPFFGTCHVAYIPNGKIIGLSKVPR
                     IVEVFSRKLQIQEKMTVEIADFLDKVLKPEGVAVVAEAYHLCMAMRGIKKAKAKMLTS
                     AMHGVFKEDERTRIEFLSFIGKHR"
     gene            2166..2348
                     /locus_tag="VI935_02315"
     CDS             2166..2348
                     /locus_tag="VI935_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24467.1"
                     /translation="MKDEVVVVTWTGHYSEFMLEDAPGFWELRGYHMYGNPWNEQRFW
                     DDDMKVWEIRERGKQK"
     gene            2712..>2889
                     /locus_tag="VI935_02320"
     CDS             2712..>2889
                     /locus_tag="VI935_02320"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLE24468.1"
                     /translation="MTIYKYTVLFEPIEEGGYNVVIPAIPEICTFGETLEEAREMAKD
                     AIHCFLESAIKAGEP"
BASE COUNT          931 a          491 c          652 g          815 t
ORIGIN      
        1 tctacgatgc catttgaaat ccaaaccctt tttcgttctt tttctagata ccttagaaat
       61 ttttcaagaa tctcttcaga gctagttttc atcgaataaa atagtaccgc agagtttaag
      121 aaataggaaa ccttacaaaa agtaggtaat aaaatttata tataaaaatg ggggtacttt
      181 tcttgattta aacatatgtg acattaactt gagaatttcc tgtgcttgct acagggtttc
      241 tactgtcatt gcaaacgcat tcgtggagtc tgcagtgaga acggataggc gggacattct
      301 tgtcccgcaa ctggatgcgg ggtttgaaaa ccccgcctat cgataaactc cctacagtca
      361 ccgaagagac catcttttcc atgctgatac cctgtggctt gccgcagtga gcttcattcg
      421 tcctggcgtc atttaaggta gtttgatatt tatataatag tttgtgctta aaattgtatc
      481 aaaaaattgg cctttctact gaccaagctt gtattctaag aaacgtcaat atggattggt
      541 cacatttatc tacctgtgat aatataataa gctatcatct aggataggaa agtagtgtcg
      601 ctattctggg gaaaagcgga aaaaaaggag aaatttatgg gttataaaac ccttcttaca
      661 aaaagggtag aattttctgc ttctcatcgg tattggaatc cgaaatggaa tgaagaggca
      721 aataaggctg tatttggcaa gtgcataagc ccatacggtc acgggcataa ttatgtcctt
      781 gaggtcaccg ttgaaggaac ggttgatact gataccggca tgattataaa catttatgat
      841 ctgaaaccgc ttataaatga ggttcttaag gattttgatc acaagtttct aaacgaagat
      901 aatccatact ttagggattt aatccctaca acagaaaata tcgcacgagt cttattgggt
      961 ttgatagaaa aaaagcttaa agaaacaaca gactgcaaac tctataggat tagactttac
     1021 gaaacacctg atttatttgt tgattattgg agggaataat gagaagggag atatgcactt
     1081 tgacgaagat ttaccatttt tctgctgccc acagacttca tgcgacaagg tattcggatg
     1141 aggaaaaccg gcgaatcttt ggaaaatgta acaatcccaa agggcatggg cacgactacc
     1201 atatcgaggt taaggtaact gggaatattg accctgaaac tgggatggta ataaatctaa
     1261 gtgagttaga cgaaatgatg aaggatattg tagaagaact tgaccacaca aggcttgata
     1321 ttgaggttcc tcacttcaga gaatttgttc cctctgggga gaacatagca aagtacatct
     1381 ggatgaggct tcagcctaag atgaagaaag gtgcacctct tcatataaag ctctgggaga
     1441 cacaggataa ctattttgag tattttgagg aggataatta ttcagctaat gagtaaaacc
     1501 ttagccacag agagcacaga gtacacagag ataggaaaaa caaaattcat ttttttaaat
     1561 cttttctctg tgtcctctgt ggctaaatag ttgccgagga ggaatggtag caatatgagt
     1621 attgaaaaag ctataaagga aatacttctt gagattggag aagaccctga gcgtcagggt
     1681 cttaaaaaca cacctgatag aatagctaag atgtataaag aactcactgt aggatacagg
     1741 gtgtctcctc aggagataat taatggcgca atctttgaca taaaatatga tgagatggtg
     1801 attgtaaagg atatagagtt ttacagcctt tgcgagcatc atctactccc ctttttcggc
     1861 acatgtcatg ttgcctatat accaaacgga aaaataattg gactcagtaa ggtcccaaga
     1921 atcgtggagg tgttctcccg taagcttcag attcaagaaa aaatgacagt agagattgca
     1981 gattttcttg ataaggttct taagccagaa ggcgtggcgg ttgttgcgga agcgtatcat
     2041 ctctgtatgg caatgagggg gattaaaaag gcaaaggcta agatgctgac cagtgcaatg
     2101 catggggtat ttaaagagga cgaaaggaca aggatcgagt ttctaagctt tatagggaag
     2161 catagatgaa ggatgaagtt gtagttgtaa cctggacagg tcactactca gaatttatgc
     2221 ttgaagatgc accaggattc tgggaactca ggggctacca catgtacggt aatccatgga
     2281 atgagcaaag gttttgggat gatgatatga aggtatggga aattagagaa agaggcaaac
     2341 agaagtaaat taagggaaca aaaaggggga cgcctttttc gccaattatt cttagaccgt
     2401 cccataggtc tgggaaattt atggtcacct atagaggtaa tagtgaagag aagataaagt
     2461 gattcctctg tctgtttttt taattcaaaa actaaactcc ttccatccag tcgatttttc
     2521 tatatcagga agcaccggac tcattaccgg tcttcagtca cctattggca gcattcacta
     2581 aaagggcctg gacgataatc ttggaggcga ctacctcagt ccttttttgt tcttatgcaa
     2641 accttgacga ataatcctga aagtttatgc ttacaagcac atatatggaa tttttacctt
     2701 aagttgaagg catgacaatt tataaataca cagtgctttt tgaaccaatt gaggaaggtg
     2761 gttataacgt cgttatacca gccattccag aaatatgtac cttcggcgag accttggaag
     2821 aggcaaggga gatggcaaag gatgctattc attgtttcct tgaaagtgcg attaaggcgg
     2881 gagaaccta
//
LOCUS       DATDRL010000065         3619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1651712, whole genome shotgun sequence.
ACCESSION   DATDRL010000065 DATDRL010000000
VERSION     DATDRL010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651712"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VI935_02325"
     CDS             complement(<1..582)
                     /locus_tag="VI935_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLE24469.1"
                     /translation="MGKKYSREDQSSISLFKPPKSSIPLAERIRPRDFSEFVGQSHLV
                     GSDGTIRKMIESGNIRPMILWGPAGSGKTTLARIIANKIEADFFQVNAISSGVKEMRE
                     IISHAKSSLEFGKRTILLIDEIHRFNKAQQASLLQSVEDGTLILIGATTENPSFEVIS
                     PLLSRCHLYILEPLVESDLESILSRALTQDEFLR"
     gene            complement(699..1307)
                     /locus_tag="VI935_02330"
     CDS             complement(699..1307)
                     /locus_tag="VI935_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24470.1"
                     /translation="MNIIGILAYGSLIDDPGQEIQAVIAKRVEGVETPFKVEFARKSR
                     RRDYAPTLVPVEEGGAYVKSVILVLKEHVSEAEAICMLWRRETNQVGSQRTYKPSANP
                     GPNTVIIDRLENFQGIGVVFYTKISANIDNPTPKKLAELAIQSARAKAGAEGRDGISY
                     LISAKRNGIMTPLMSEYEKEILRRTGTQSLEEALKFLRADSD"
     gene            1536..2504
                     /locus_tag="VI935_02335"
     CDS             1536..2504
                     /locus_tag="VI935_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24471.1"
                     /translation="MHNKKHHKIERRIKEYISKLLSDEAGLFGRFVPYYAYGTQIAIS
                     LELLLILENSEIYLSKLLEERELLQSQARKNFLINSVTALENFLIYWIINLFDWEFEV
                     LEKTNGEKYFLNWEGIKEVIEAKGNLGLLVEEIKEGEITRWKNPAHIIISTFSFQNLN
                     DINFVMTKITGRNFFKEVENMESEFADLVCPGSEAIKKLIDNYTKEIAKINLEKNDYI
                     EQGDWKSQLIDLKKKLLEKQGKFILKKEFPNWKEKLGYLFELRNEFVHFVKSRKLSPG
                     EVSEFLVALDYFVQAVEHFLFNEIRDPIVMELLYLDLKEKEEKNQT"
     gene            complement(2810..3121)
                     /locus_tag="VI935_02340"
     CDS             complement(2810..3121)
                     /locus_tag="VI935_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013798425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HLE24472.1"
                     /translation="MRTLEEVKNILAENKEGLKEKFKVKRLGIFGSYARNEQSEDSDL
                     DLLIAFEEESVGGWEYIGLMMDLEEFMENALEIKVHLATERQAMNSDKWEYVKKDLIY
                     V"
     gene            3215..>3619
                     /locus_tag="VI935_02345"
     CDS             3215..>3619
                     /locus_tag="VI935_02345"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HLE24473.1"
                     /translation="MEIKLLKQLCEAPGLPGAEEPVKEIVISNLKEHTEEIKIDALGN
                     VIAHIPGDGPRLLLDAHMDEVGFMVKHIDQRGFIRVIPLGGIDPRVFYGQRVVVWGKK
                     PLVGMVAALPPHIVNKEHEKEAPEVEDCTIDLG"
BASE COUNT         1154 a          677 c          647 g         1141 t
ORIGIN      
        1 tctcaaaaat tcatcttgag ttaaggccct gcttaatata ctttcaagat cgctttcaac
       61 taacggctcg agtatataaa gatggcacct agaaagtagc ggagatataa cctcaaatga
      121 tggattttcg gtagtagcgc cgataagaat cagtgtgccg tcttcaacac tctgtagaag
      181 tgaagcttgc tgtgctttat tgaagcgatg aatttcgtca atcaataaaa tggttcgctt
      241 tccgaattca agagagctct ttgcatgaga gataatctcg cgcatttctt tgacacccga
      301 cgaaattgca ttaacttgaa aaaagtctgc ctctatctta tttgcgataa ttcgtgcaag
      361 cgtagttttg ccgcttcccg ctgggcccca taaaatcatt ggacggatat taccgctttc
      421 aatcatcttg cgaattgttc catcagaacc aacaagatga ctttggccta caaactcact
      481 aaaatccctt ggtcttattc tttcagctag gggtatagag gattttggcg gtttaaaaag
      541 tgaaatgcta ctctgatctt ctcttgaata tttcttaccc atatccctat ataaagaaga
      601 ttagagtgag atgaaatgga aatcaaatgg taaatctaat atctcgcatg tttgcgaagt
      661 gctgaaaaca cttaggttag cgaaccgcaa acattttatt agtcgctatc agcacgtaaa
      721 aatttcaaag cttcttcaag actctgcgtt cctgttcgtc gaagtatttc tttctcgtac
      781 tccgacataa gaggagtcat gattccatta cgtttagcgg aaatcaggta acttattccg
      841 tctcgtcctt cagctcctgc tttagcacgg gcgctctgta tagccaattc agccagcttt
      901 tttggtgttg gattgtctat atttgccgaa atctttgtgt aaaacaccac tcctatgcct
      961 tggaagttct ctaatctatc tataatcaca gtgtttggac caggattagc tgatggtttg
     1021 taagttcgct gactaccgac ttgatttgtt tcccttcgcc ataacataca tatagcctct
     1081 gcctccgata catgctcttt caaaactaag atcacagatt tgacgtaagc tcctccttct
     1141 tcaacgggaa ctaatgttgg agcatagtct cgtctcctgc tttttctggc aaattctact
     1201 ttgaatggcg tttccacacc ttctacacgc ttagcaatta cagcttgtat ttcttgtcca
     1261 ggatcgtcta taagcgaacc ataagccaag ataccaataa tgttcatatt tgaatttagc
     1321 ttatattaag agtaagtact atattaacaa taatccttcc ctataagctc aagaaatcat
     1381 ttttttttca gtttaaccta cctcagagag ttctgcatag caattataat gaaccctatc
     1441 tgctttagaa tctagacaac cctataaact taatgtattt tgaggacaca gcaaatgttt
     1501 gttactttaa ataaaaagaa gctaatttaa aacatatgca taataaaaaa catcacaaga
     1561 ttgaaagaag aattaaagaa tatattagta aattattaag cgatgaagca gggttatttg
     1621 gacgtttcgt tccttactac gcctatggta ctcaaatagc tatttcccta gagttacttt
     1681 taatccttga aaattcggaa atttatctca gcaagttact agaggaaaga gaacttctgc
     1741 aatcgcaagc tagaaaaaat tttttaataa actctgttac agctttagaa aactttctta
     1801 tttactggat tataaactta ttcgactggg agttcgaggt tcttgagaaa actaacgggg
     1861 agaaatattt tttgaattgg gaaggaataa aggaagttat agaagcaaaa ggcaatcttg
     1921 gattactggt agaagaaata aaggaaggag aaatcacgag gtggaaaaac cctgctcata
     1981 ttataatttc aactttctcg ttccaaaacc ttaatgatat caacttcgta atgacaaaaa
     2041 ttaccgggag gaattttttt aaagaagtag aaaatatgga aagtgagttt gctgatcttg
     2101 tgtgtcctgg ctcggaagct ataaaaaagt taatcgataa ctatacaaag gaaattgcga
     2161 aaattaattt agaaaaaaat gattatattg agcaaggtga ctggaaatcg caacttattg
     2221 acctgaaaaa aaaattgctc gagaaacaag gtaagtttat tttaaaaaag gaatttccta
     2281 actggaaaga aaagttagga tacctttttg aactacgaaa tgaatttgtg cattttgtta
     2341 aatcaaggaa attaagccca ggcgaagtat cggaattctt agtggcattg gattattttg
     2401 tacaagcagt ggagcatttt ttatttaatg aaattcggga cccaatagtc atggaattat
     2461 tgtacttaga tctaaaggaa aaggaagaaa agaatcaaac atgattagtt aataatcacg
     2521 ctaaaattgt ggcttcgtat ttagttaaat taatccctct ttctaacaaa atatttaatt
     2581 caaacttgtt gtggaaacta aacctctaaa ataggtacat aaggtccaag aagcgatacc
     2641 tggagttgaa gagccagttt aagaaatcgt tatctccaat tttgtcgtgg agtggattgt
     2701 ctctttaagt caaactaagt ccagttgacg agcaaagcct caatccctaa accttcaatt
     2761 atgtcctgtg gatttaaact taacatccct ctgagttttt ctacattctt caaacataaa
     2821 ttaaatcctt tttcacatac tcccacttat cggagttcat tgcttgcctt tcagttgcaa
     2881 gatgtacttt tatctctaat gcattttcca taaattcttc taaatccatc atcagtccta
     2941 tatactccca ccctcctaca gactcttctt caaatgcaat tagcagatct aaatcactat
     3001 cctcactttg ttcatttctt gcataagacc caaagattcc aagtcgtttt accttgaatt
     3061 tttccttcaa tccctcctta ttttctgcta agatgttttt aacttcttca agagtcctca
     3121 tcttaactaa caaatcaaaa acttaacaag agtgtaaaac aattcgtatt gcattggcaa
     3181 atctatcttt gctagtatat aatcaaaatt tattatggaa ataaagcttc taaaacaact
     3241 ctgcgaggcg ccagggcttc ctggggctga agagcctgtt aaggaaatcg taatttctaa
     3301 tcttaaagag catacggaag aaattaaaat agatgccctc ggcaacgtta tcgcccacat
     3361 tccaggtgat ggtccaaggc ttcttttaga cgctcatatg gatgaagtag gctttatggt
     3421 taagcacata gaccagaggg gatttataag agtaattcct cttggcggga ttgacccccg
     3481 ggttttttat ggtcaaagag tagttgtgtg gggtaaaaaa ccacttgtag gaatggtcgc
     3541 cgctctaccg ccacatattg taaataaaga gcatgaaaag gaagcgccag aggttgagga
     3601 ttgtactatc gaccttgga
//
LOCUS       DATDRL010000066         6756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2551048, whole genome shotgun sequence.
ACCESSION   DATDRL010000066 DATDRL010000000
VERSION     DATDRL010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2551048"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1112
                     /locus_tag="VI935_02350"
     CDS             90..1112
                     /locus_tag="VI935_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HLE24474.1"
                     /translation="MFTPIYLLPQYNPKDRTKYEDRWNDPDNQHIQDEVLRMIRNGAG
                     EDFLERVFDQGFLEDMWDLKGMDIFQEEIDFPRGDNFEAIDFSYASFSHSKFKNATFV
                     NNIFHFTKVLNCEFVNCIFAFGEFYGVSLEKTRFINCDFIEYHCITNCDFKEVKFENC
                     FIPKRLFFNCKFDEQTIINEPIDKPQRMSGDSFKLSNSDLAEIFKGIKESYTAGNVIK
                     QSRLYFYRERQSITRYNANNWRDKIGGYFLELIAGYGIKPFRVLLNMLTIFFIFSIVF
                     IAKVGASKGLLLSAGAFFTFGANTNYLQTLGSFFKIIYIAESFFGISLMALFITVLAN
                     YWFREK"
     gene            complement(1299..2318)
                     /gene="pheS"
                     /locus_tag="VI935_02355"
     CDS             complement(1299..2318)
                     /gene="pheS"
                     /locus_tag="VI935_02355"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HLE24475.1"
                     /translation="MKEKLEEIRIEAESELKKAPDEASLQNVKAKYLGRKGVITEFLK
                     RMPEVSESERPEMGRLINEVKSFIENLFEKRLDEIKEERKTSVLFKERVDITLPGRGI
                     RLGGKHPITQVMEEIISIFERMGFEVVEGPEVELDYYNFEALNIPKDHPARDMQDTFY
                     ISDEIVLRTHTSPVQIRVMEKQKPPVKIIAPGKVYRCDSDISHTPMFHQVEGLLVDEH
                     VTFADLKGVLTEFAKLMFGANIGVRFRPSFFPFTEPSAEVDIECVICGGKGCRVCKNS
                     RWLEILGCGMVDPEVFKSVNYDPERYTGFAFGMGVERIAMLKFGINDIRLFFENDIRF
                     LRQFS"
     gene            complement(2532..3038)
                     /locus_tag="VI935_02360"
     CDS             complement(2532..3038)
                     /locus_tag="VI935_02360"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HLE24476.1"
                     /translation="MDYMVFSIFLIVALFGLVSLIFGLPGNFIILADSLLYGWYGGFK
                     EITFKVILILIILVIIGEVLEYGLGILGAKKRGATKGAIIASIIGGIAGAIIGTPFLF
                     GIGSIIGAFLGAFAGAFFLEYLQGKGVDQAIESGWGAFLGRVGGAISKGFIGIMMIVI
                     TIVSVVRN"
     gene            complement(3326..4081)
                     /locus_tag="VI935_02365"
     CDS             complement(3326..4081)
                     /locus_tag="VI935_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLE24477.1"
                     /translation="MSKRLTKLILFYFILIGLWQGLISLRLWPEYIFPSPFGVLKTLV
                     AGLEDKTFIIGILVSMKRILIGYGFSMVVGLCIGLLIGRIKTLDETFGSLVLGFQALP
                     SICWLPLALLWFGLSEYAMLAIVILGSLFSIIIGADTGVKNVPPIYIRAAKTMGAKEI
                     QLYTKVIIPAALPSIISGFKQGWSFAWRSLMAAELLYVGLGLGHLLMMGRELNDMNQV
                     IAVMIVIIIIGIVVDRLIFAKLENKVRERWGLA"
     gene            complement(4078..5166)
                     /locus_tag="VI935_02370"
     CDS             complement(4078..5166)
                     /locus_tag="VI935_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLE24478.1"
                     /translation="MIKGTHQLVQTEELKNFDKLKPNKIIDVRGAVCPHPLNLIRDSI
                     ISLKDKEILQAICDHEPTAKENAPRFCTRRGYPLQVTEQNGVWEIFIQKIEKTQPRLR
                     VLNVSKVFKTNHGKVNALDNVNLTIQEGEFVCIVGPSGCGKSTLLNLIAGLDKPTQGE
                     IWSNGNLVTGPGSDRVVVFQEGALFPWLTVMQNVEFGLRLRNVAHNIRRTLALDYLKL
                     VGLDEKFHNSFIHQLSGGMKQRVAIARALVMEPAILLMDEPFAALDVYTRETLQEELQ
                     NIWTESHKTIVFVTHNVQEAVLLGDRVVVFARNPGRVKAEYEIDIARPRKIDHPEVFL
                     NTKWITDDLREEMEEWNYEADTKKVVHR"
     gene            complement(5159..6223)
                     /locus_tag="VI935_02375"
     CDS             complement(5159..6223)
                     /locus_tag="VI935_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLE24479.1"
                     /translation="MKYLAWTLLADLAIGLLFLGLGAIAKEPKTTIRVGHFPNVTHAQ
                     GVIGQANGWFEKALGKDTKIEWKIFNAGPSAIEALFAGELDLTYIGPNPAINGYVKSK
                     GEALRIVAGAVSGGAGLVIRADSGINTVKDFDGKKIASPQLGNTQDVALRGWLKDNGY
                     EVKEKGGTVQVIPLANPDQLTLFLKKEIDGAWTIEPWVSRLILEGGGKLFLDEREIWP
                     DGKFVTTHLIVSTKFLKEHPDLVKKWIATHVELTEWINKNPEEANKQLNEEIKKETGK
                     ALPQNVLDESLKRIGVTYDPIASSLYKSARWAFQQGFLGKEQPDLSNIYDLTLLNEVL
                     SKKGLQPIKLDVKTQAVKND"
     gene            complement(6519..>6756)
                     /locus_tag="VI935_02380"
     CDS             complement(6519..>6756)
                     /locus_tag="VI935_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HLE24480.1"
                     /translation="GDKYFRFPVFKTQGVYYSILAILYAIRADLPEKRFVIHLGWPTS
                     SWIDRLAVGLFVFNLMKLPKLFPEFEFVIEHGSV"
BASE COUNT         2034 a         1480 c         1185 g         2057 t
ORIGIN      
        1 tcgcccaacc aaatcactct tatttacatc aacgtccttg gcaacgagaa gcaggagata
       61 aagacaacaa aggattttaa agagtaaata tgtttacgcc catatattta ttgccccaat
      121 acaatccaaa agatagaact aaatatgagg atcgttggaa tgaccccgac aatcaacata
      181 ttcaagatga agttctcagg atgataagaa atggtgcagg agaggatttt ttagaaaggg
      241 tttttgatca aggattttta gaagatatgt gggatttaaa aggaatggat atttttcaag
      301 aagaaataga ttttcctaga ggcgacaact ttgaagcaat agatttctcc tatgcctcct
      361 tctctcattc gaaattcaaa aatgcaacct tcgtaaataa tattttccac tttacaaaag
      421 tattaaactg cgaatttgtc aactgtattt tcgcatttgg agaattctac ggtgttagtt
      481 tggaaaaaac cagatttata aattgtgatt ttatagagta tcactgtata acaaactgcg
      541 attttaaaga agttaagttt gaaaattgtt tcatacctaa aagattattc ttcaactgta
      601 aatttgacga gcaaactatc ataaatgaac ccatagataa accccaaaga atgtcagggg
      661 acagttttaa attaagtaac agtgaccttg cagagatatt caagggaata aaagagagtt
      721 atacggctgg gaatgtaatt aaacaatcga gactttattt ctacagagaa agacaatcta
      781 ttaccagata caatgcgaat aactggagag acaaaatagg tggctatttt cttgagttaa
      841 tcgcgggtta tggcatcaaa ccatttcggg ttctcctaaa tatgctaacc atattcttta
      901 tcttctcaat agtttttata gctaaggtcg gggcttcaaa aggacttcta ctgagtgcag
      961 gtgctttttt tacttttggt gcaaatacca attaccttca aactctgggt tctttcttta
     1021 agattatcta tatagcagaa tctttcttcg gcatatcact gatggctttg tttattaccg
     1081 ttttggctaa ttactggttt agggagaaat aacgttacgc ctgagagcca aaacttcaaa
     1141 acaaggcttt cagaaaatga gaaagaacac gaaaacacaa gattatcaat cgggggattt
     1201 cgcacacagt gcgtgcagaa ttgcttccaa tttctcagac acaatatgaa atttccgaga
     1261 ctctgtctcg gaatatgtca gagacaaaaa tcccaaatct aagaaaactg cctcaagaac
     1321 cttatatcgt tttcaaaaaa caacctgatg tcgtttatgc cgaatttgag catggcgatt
     1381 cgttcaacgc ccattccaaa tgcgaaccct gtgtatcgct cggggtcgta gttaacgctt
     1441 ttaaatacct cagggtctac cattccgcag ccaagtattt ctagccatct ggagttctta
     1501 caaaccctgc atccctttcc tccgcagatt acgcactcaa tatccacctc tgcgctcggt
     1561 tctgtgaaag gaaagaagct cggtctaaat ctaaccccta tatttgcacc aaacattagt
     1621 tttgcaaatt cagtaagtac cccttttaaa tctgcaaacg ttacatgctc atcaactaaa
     1681 agcccctcta cctgatgaaa cataggtgta tgtgatatat cgctgtcaca tctataaact
     1741 ttccctgggg cgattatttt cacaggcggt ttttgtttct ccatcaccct gatttggact
     1801 ggggaggtgt gagttctaag aactatttca tctgaaatgt aaaatgtgtc ctgcatatca
     1861 cgcgctggat ggtcctttgg aatgttgagg gcttcaaaat tatagtaatc aagctcgact
     1921 tccggacctt ctacaacctc aaatcccatt cgctcgaaaa ttgagataat ctcttccatc
     1981 acctgagtta ttggatgctt cccaccaaga cgaatgcctc gaccaggaag cgtaatgtca
     2041 acacgctctt taaaaagcac acttgttttt ctctcttctt ttatttcatc aagtcttttc
     2101 tcaaataggt tttcgataaa actcttcacc tcgtttataa gacgccccat ctcaggtctt
     2161 tccgactcag aaacctcagg cattcttttt agaaattccg taataactcc ctttcttccg
     2221 agatacttcg ctttgacgtt ttggagagaa gcttcgtcag gagctttctt tagctcactc
     2281 tcagcctcaa tccttatttc ttcaagtttt tctttcaatt ttagctccta aattagtata
     2341 cgaacctcaa tattgtaggg gcaactcttg cggttgccca agaaaacagg gcgggtgcaa
     2401 gacccgcccc tacagttccg cgtagacagg cgggtttaga aaaccctgtc tatagtagaa
     2461 agccctttga ctctaatcgt gttccataat gtgcttaaga ccccaatgca tgtaactgca
     2521 ccctacagat ttcaatttct cactacagaa acaatcgtta ttacaatcat cattatacct
     2581 ataaaaccct ttgaaatggc tccacctact cttccaagaa aagctcccca tcccgattct
     2641 attgcctgat ctactccctt tccttgtaag tactcgagaa agaaagcccc ggcgaatgct
     2701 ccgagaaaag cacctataat tgaacctatc ccaaatagaa aaggcgtacc aattatagca
     2761 ccagcgattc cacctataat tgaagcgata attgcacctt tagtcgcccc acgctttttt
     2821 gctccgagta ttccaagtcc atattctaaa acttcgccta tgataacaag aataattaag
     2881 attaaaatta ccttaaaagt tatctcttta aacccgccgt accaaccgta tagaagagaa
     2941 tctgcaagaa taataaaatt ccccgggagt ccgaatataa gcgaaactaa accaaaaagt
     3001 gctacaataa gaaaaatact aaaaaccata tagtccattt ctctacttag aaaaatatat
     3061 tacctaatat actcgttatt ggaatttctt agcggtatgg ggtaaggagc aaagagtcag
     3121 caaaaaaaag cttaaatcat aactaggaaa tttcttgtta ttccatctcc tactcttgtt
     3181 tttttctaat ctcaacttac agtatcctaa ttactttttg tgaaaagtac aaagaaaaaa
     3241 gcctcgaagg ttattgaagt gaaaagctag taaaaactaa gtcatatcag gaaatagaac
     3301 catacaataa agacaacttt aaacactagg ctaatcccca tctttctctg actttgttct
     3361 ctaactttgc aaaaattaat ctatctacta ctattccaat tatgattatt acaatcatga
     3421 cagctataac ctgattcata tcgttgagtt ctctacccat cataagcagg tgaccgagac
     3481 cgagaccaac ataaagaagt tctgctgcca ttagggaacg ccacgcaaaa gaccatcctt
     3541 gtttaaaacc acttataata gatgggagtg ctgctggtat aataaccttc gtgtacaatt
     3601 gaatttcctt cgctcccatt gtttttgcag ctctaatgta aataggagga acatttttta
     3661 caccggtgtc ggcaccgatt atgattgaaa agagcgaccc aagtataaca atcgcaagca
     3721 tcgcatactc actcaaccca aaccataaaa gggctagcgg aagccagcat atgcttggaa
     3781 gcgcttgaaa acctaaaacc aaggagccaa atgtttcatc aagtgttttt attcttccta
     3841 taagaagacc tatgcacagt ccgactacca ttgagaaccc atatccaata agaattctct
     3901 tcatactgac taaaatgcct attatgaagg ttttgtcttc caatcctgca acaagtgttt
     3961 tcaatacacc aaatggtgat gggaaaatat actcgggcca aagtctgagc gatataagcc
     4021 cctgccatag gcctatgaga ataaaataga agagaattag tttggtcaat cgcttgctca
     4081 tctgtgaacc accttctttg tgtcggcttc gtagttccac tcttccattt cttcccttag
     4141 gtcatctgta atccactttg tgttcaaaaa cacctctgga tgatctattt ttctcggtct
     4201 tgcaatgtca atctcgtatt cagcttttac tcgtcccggg tttcttgcaa aaactaccac
     4261 tctatctcca agaaggacag cttcttgaac gttatgtgtg acaaaaacaa tagttttatg
     4321 tgattcagtc cagatgtttt gtagctcttc ctgaagtgtt tcccgtgtat aaacatccag
     4381 tgccgcaaag ggctcatcca tgagcagaat cgctggttcc ataactaaag ccctagctat
     4441 tgccacacgc tgtttcatac cccctgatag ttggtggata aaagagttgt gaaatttttc
     4501 atcaagccct acgagtttca ggtaatcaag tgcaagggtt ctccgtatat tatgagcaac
     4561 attgcgcaat ctaagaccaa attcgacatt ttgcatcact gtaagccagg ggaaaagcgc
     4621 accctcttga aacacaacaa cacggtcaga accaggtcca gtgacgagat ttccgtttga
     4681 ccaaatttca ccctgcgtgg gtttgtcaag tcctgcaatt aaatttagga gcgtagactt
     4741 accgcaccca gaaggaccaa caatacaaac aaattcccct tcttgaatgg tcagattgac
     4801 attatctaga gcattgacct ttccatgatt cgtcttgaat acctttgaca catttaacac
     4861 ccgaagcctt ggctgtgttt tttcaatctt ttgaataaaa atctcccaga cgccgttttg
     4921 ttcggtaacc tgaagtgggt aaccccttcg tgtgcagaat ctgggagcgt tttccttggc
     4981 tgtgggctcg tgatcacata tagcctgaag gatttctttg tccttaaggc ttattattga
     5041 atctctaatc aaattgagtg gatgagggca tactgctcct cgaacatcga ttatcttgtt
     5101 cggttttaat ttatcaaaat tttttaactc ttcagtttgt accaattgat gcgttccctt
     5161 aatcattttt caccgcttgc gttttaacat ccagctttat aggttgaagc cctttctttg
     5221 atagcacttc gtttaacaag gttagatcat aaatgtttga taggtctggc tgctctttac
     5281 ccaggaatcc ttgttggaag gcccaacgtg ccgacttata aagagagctt gcaattgggt
     5341 cgtaggtaac acctattctt ttcaatgatt catcaagaac gttttggggc agggctttcc
     5401 ccgtctcttt ttttatctct tcgttaagtt gcttatttgc ctcttcgggg tttttgttta
     5461 tccactctgt aagttctaca tgagttgcta tccatttttt aactaaatcc ggatgttctt
     5521 ttagaaactt tgtgctgaca ataaggtggg tagtaacaaa cttcccgtca ggccaaattt
     5581 ctctttcatc taagaaaagc tttccgccac cttctagtat cagccttgaa acccagggtt
     5641 cgatcgtcca cgctccatca atttcttttt tgagaaataa agtcaattga tctgggtttg
     5701 ctagagggat aacctgcacg gtgccaccct tttcttttac ctcataacca ttatctttaa
     5761 gccagcctcg aagcgctacg tcttgagtgt tacccaactg tggagacgca atcttcttgc
     5821 catcaaaatc tttcacagta tttatacctg aatctgccct aataacaagc cctgcgccgc
     5881 cacttactgc cccagcaaca atgcgaagag cttctccttt agattttaca taaccgttaa
     5941 tcgctggatt tggacctata tacgtgagat caagctctcc agcaaataat gcctcaatcg
     6001 ctgatggacc agcattaaat atcttccact cgatctttgt atcttttccc aaagctttct
     6061 cgaaccaccc gtttgcctgt ccgattacac cttgggcatg ggttacattg gggaaatgac
     6121 ctacgcgtat ggttgtcttg ggctcctttg caattgcccc taaccctaaa aatagtaagc
     6181 ctatagctaa atcggctaac aaagtccatg ccaggtattt catttgatta tcttcctcct
     6241 tagtttggtt tcagatcgta gagtgccatt aactggcaag gagggtgcca aataaaaagt
     6301 tccctttatt ttcgggtaga tagaaagaaa gtgtagatac gaaaagatta gctactaacc
     6361 aagttatggt tactaatcaa tcaagctgta tattgttgtg ccaaaccaaa ggttatgata
     6421 ttagatatga actacaagaa aaggctatat atggttttta aaacttaaga taattccccc
     6481 tttataatag agattgttat gtttggaatc catccgcttc atactgaacc atgttcaatt
     6541 acaaattcaa actctggaaa cagctttggg agtttcataa gattaaaaac aaatagaccc
     6601 acagccaatc tatctatcca agatgaggta ggccatccta agtgaatcac aaaccttttt
     6661 tctggaaggt ccgctctaat cgcatagaga atagcaagta tagaataata gacgccctgt
     6721 gtcttaaata caggaaacct gaaatattta tcacct
//
LOCUS       DATDRL010000067         9808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3794781, whole genome shotgun sequence.
ACCESSION   DATDRL010000067 DATDRL010000000
VERSION     DATDRL010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3794781"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VI935_02385"
     CDS             complement(<1..669)
                     /locus_tag="VI935_02385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLE24481.1"
                     /translation="MTIKTQAYLVLLILTTINFLNYIDRQIIYAVFPLIKQDMLLSDK
                     QLGLIGSAFVIVYLITSPIFGRLGDLVSRKGIIALGVGVWSLATVGASIAQNYSQLLL
                     ARSLVGIGEASYVVTAPTLISDYFGRERRGKALSFFVMALPVGSALGYILGGHLGEIY
                     SWRSAFLIVGFPGLLMVLLCLKIKEPKRGAVDDAVVYEPSGDSLQIVNLREVYIMLLK
                     NKQFV"
     gene            993..3323
                     /locus_tag="VI935_02390"
     CDS             993..3323
                     /locus_tag="VI935_02390"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HLE24482.1"
                     /translation="MHILKFRVSRAKIKLSLLFLLIASCSGAALLALPGISSSILPAV
                     IPIILFQPPPIRVPTAVLPFNSNIQSAAITPDGSKLYASDSFNNTVSVIDLSINEVIA
                     ILDAGNGPSSIQVTPDGSRAYVVNSGTDTNPGNTVSVIDISRDKVVTKVNLGSRPSGL
                     AITPNGSGVYVTGFSLLMGIVSVIDVSNNEVIRTIEVGIRPNAIAITPDSSKAYVADS
                     ASNIVSVINISTNEVSSTINVGLIPTNVVISPNGLRVYVANSGSNTVSVIDVEKSVTD
                     PLNAVIANVPVGLKPGVIAISPDGSRVYVSNLNSNTVSVIQASTNGIIATVNVGLVPI
                     GIAVAGDNSKLYVANSGTSTVSVIDIATNQVINTVEVEPNPIAVFIAPDNSRVYVANA
                     DTITVIDVPRDIVINKIFVRGGGPTNIAIAPDGSKVYLASSGRDRVSVIDVSVDRIIA
                     TINVGSKPSSMAITPDGSSLYVTNFSSNTVSVINISTNEVIDTLNVGNAPLAIAIAIT
                     PDGAKKAYVANFSSNTVSVIDVSTNSVIPTVIDVGLGPSFIAITPDGTRAYVTNVGND
                     NQSDSTVSVIDTTTDTVAATVGVGSRPVDLAITPDSTRVFVPNFLSNTLSVIDALTNL
                     VVTTVTDVGPGPSFISLTPPPDSFKAYVVNPADQSSFGETGNTVSVVDIINNTLIAAV
                     QVGSRPIDLAIRPDGSQVFVVNYFSNTVSVIDTLTNLVTATLNVGNAPLAIGIAITPD
                     GAKAYVANTVSETVSVINVLTDEVIKTITVSPRPSV"
     gene            3362..6163
                     /locus_tag="VI935_02395"
     CDS             3362..6163
                     /locus_tag="VI935_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE24483.1"
                     /translation="MKILNYISVILLICSLLTACIGVKQSDIKIKDEKAVQLVKEAIN
                     LYDKEKYDGALDLLSEAKNQARLPEDKLEIADILSKGGFGLLKKKEFNTALAYYENSL
                     DINRTLDFKPGLINNYSYIGIIYTDLGRYEDGIGYFKKALEIQEELNDKSGIAQNLNN
                     VANLYSYLGNYQESIKLLKQALGLSEEINDSTQTAKTLINLATINLRLRNYQNSIQYL
                     NSALDIAGSAKEENLKAYSLNLTGAIYRQQGDYTKALHYYEDALKINKKLGLQTEIAT
                     NLSIIGELYKELGKYDDALKYFQQSLGISESSKDRLLTAINLNFIGEVKYKQGNYDEA
                     LGLYNSSLEIFEELGFKDRIARSFNDIGYLQGEKKKLDNAIENLDKAITIYKDLGDRE
                     WVRVALFGRGVYSEEKGDLVSAEINYKEAVEVFESIRDDVSGDEEAKQLFSEVNIKVY
                     ENLVSLLIRQGKKEEALEYIERSRSKTLRDTFLKSGITSSDEKTRGLLTLYDELSRKE
                     ASINYELVNEKGKPKPNLDKVGNLVKTLAKTRKDFSQVTNELSTEHADVYKLLSIRPG
                     TFFDLEKKNKLPSDTVFIEYFITEKETYIFLMGERDLIVKRVSVTKEELNELVSLFRD
                     IIQKNKSIPSKAWRDGGTDKYTGNIKTFKDVSMRLYQYLLEPIENETRQADIVAIIPF
                     GSLHYLPFHALAKEDSDGGIEFFIEKKGLVYLVSTSANYLDLVLRDGKKREIGSVVAF
                     GNPDLGEPELVLPYSEEEVLVIKEMFPNATVFLKGDATKDNFKRSWGRHEIVHMAAHG
                     LIQDEPSILLAPLGSGSLTLSDITGLQPVRNTQLVVLSLCDAAIEGNGSNPTGGELNS
                     VALAFSSVGASSVIASLWRVDDRATSQLMEKFYRNLKIEGKFSYEALRKAQLDMLRRS
                     DKYGQPFYWAPFVLVGVWE"
     gene            6379..6975
                     /locus_tag="VI935_02400"
     CDS             6379..6975
                     /locus_tag="VI935_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24484.1"
                     /translation="MKFEKENEILGAVATSLIERKEGMCLTDEEFALYIEGRLSNDKR
                     KAMISHFVSCRDCRERLTIPIPHFEEAIEPNSIERLLAPLWRPLIVAPIAAVVIALLT
                     FTLFVYQKPEDITGERLRGANLVALKQLDITSNLLIIIKEGDEQEFKNELIKELPPDV
                     KVSRLIIEDIKQLKGSKEGDRIILILYNDGSLKVKRGE"
     gene            complement(7011..7799)
                     /locus_tag="VI935_02405"
     CDS             complement(7011..7799)
                     /locus_tag="VI935_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24485.1"
                     /translation="MDKKILSEEDILFVRDICTRSFQRNKDFANDCFLYVWEKLNEDD
                     SRRLKAFKGESKFRTFLYSVTNKLIIDFRRSQFGYKVLPKFFWVFDEINRHIFKLFFY
                     QSSTRTWVENEIRAEFKISQNEAQSRVDKVEKRITESRLRLETTKEIQLVMLGEDVNA
                     LQSEYIGANPEQNIIMAEAEEKRQKVLKALKEEVQKLEAEDALILQLYFEQNLTAKEI
                     TYAIPGIKDKGVYKKIERILKNLRKQIKEKGITDEDINDIFERL"
     gene            8333..9466
                     /locus_tag="VI935_02410"
     CDS             8333..9466
                     /locus_tag="VI935_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLE24486.1"
                     /translation="MNSLNQRVRSSDSQFKSFSNPISHTPRNTNKQRISNLDQKLIQT
                     YFKEVGSEPLLTATEEIKIATKMKKCQIRAKELKTSIERSLGISLGNNIEKSMHVLKE
                     ISGNGSDIPKSEKSSKQLSRLITLFKAYLEKGAQLRNIFIKSNLRLVVSIAKGYTGYG
                     LPFLDLIQEGNIGLIKAVERCDYTKGYKFSTYACWWIHQSICRAITDQTRTIRVPAYI
                     IQKSGKVRDIYSSLEEKTGRRPLPEEISKEVNISSEGVKRVLRANEKVVCLDSPVWQG
                     EKTTLMDSMPDPNSEPPDSLIAAASLPQSVDEALLILSPREREILKMRFGINYENPVT
                     LDEIGKGLDLTRERVRQIEKRSLEKLRKSDFAPALRSLMEVCQ"
     gene            9597..>9808
                     /locus_tag="VI935_02415"
     CDS             9597..>9808
                     /locus_tag="VI935_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24487.1"
                     /translation="MLRNPKTAEVKTPLKNIGFATAAMSRCLDSSIAAPVIRGVLSRK
                     ENLAASSLLKFLIKPPVIVIPERETPG"
BASE COUNT         3160 a         1735 c         2003 g         2910 t
ORIGIN      
        1 tacgaattgc ttattcttga gaagcataat ataaacttcc cttaggttta caatttgtag
       61 tgagtctccg gaaggctcat atactactgc atcgtcaaca gcgcctctct taggctcctt
      121 tatcttcagg caaagaagaa ccattagaag cccgggaaac ccaactataa gaaatgcaga
      181 cctccaactg taaatctcac ctaggtggcc tccgagtata tacccaaggg cactacctac
      241 aggtagtgcc atcacaaaaa aagaaagagc ctttcccctg cgttcccttc caaagtagtc
      301 agatattagg gttggagcag ttacaacgta agaggcttca cctatgccaa caagcgaacg
      361 cgcaagaaga agttgggagt aattctgtgc aatgctggct ccaactgtag ccaaactcca
      421 gactcctacc cctagagcaa ttatcccctt tcgggacaca agatcaccca gcctcccaaa
      481 gattggtgag gttatcagat aaactattac aaaagccgaa cctataagcc caagctgttt
      541 gtctgagaga agcatatcct gtttaataag ggggaatacg gcataaatta tctgtctgtc
      601 aatgtaattt aaaaagttta tcgtcgtcaa aataagaagt accaaatagg cttgagtttt
      661 aatagtcaat taactttcca caagtggttt tatttagaaa gatatgttag tcaagcggat
      721 agattataac aaggttcttt gcaaatcgat actaaaccgg gtaattccta tctcaccatg
      781 atcatttgtt tttggccatt taaagttttt tatctggttc agggaaattc cctcaatttt
      841 tcgtctaaaa ttataagcaa aagtctatga aaactattta ttggaataaa aaggattaaa
      901 ttaattaaaa cttgtctgtc gatttttatg tattatatat agcttgttta ttcaataatg
      961 gggagtaata gcttcaaaag acggtttgaa aaatgcatat actaaaattc agggtaagcc
     1021 gtgcaaaaat aaagctaagt cttttatttc tccttatagc ttcttgttca ggtgctgcac
     1081 ttcttgccct gcctgggatt tcttcgagca ttctaccagc tgtcatcccc atcattcttt
     1141 ttcaacctcc cccgatccgg gttcccacgg ctgtcctacc atttaattca aacattcaat
     1201 ctgcagcgat aaccccagat ggctcaaagc tatacgcaag cgatagtttt aacaatacag
     1261 tatccgtaat agacctgtca ataaatgagg ttattgcaat actagatgct ggaaatggac
     1321 ccagcagtat acaagtaaca ccggatggct ctagggctta tgtggttaat tcaggcactg
     1381 atactaaccc tggcaacact gtgtctgtaa tagatatatc tagggataaa gttgttacta
     1441 aagtgaatct tgggtcaaga cctagtggtt tggcaataac accgaacggt tccggggtat
     1501 atgtgacagg tttcagcctt ctcatgggta ttgtatcggt aattgatgtt tcaaataatg
     1561 aggttatcag gaccatagag gtcggtatta gaccgaatgc aattgcgata acccctgata
     1621 gttcgaaggc ttatgtagct gattctgcaa gcaatatagt atcggtgata aatatatcaa
     1681 caaatgaggt tagttctacg attaacgtgg gcttaatacc tacaaatgtt gtgatatccc
     1741 ctaatggttt aagggtttat gtggctaatt ctggtagtaa tactgtatct gtaatagatg
     1801 tagaaaaatc tgtaacagat ccattaaatg cagttatcgc taacgtgcca gttggcctca
     1861 aaccaggtgt gatagcaata tcgcctgacg gttctagggt ttatgtttcc aatcttaaca
     1921 gcaatacggt atcagttata caagcatcaa ctaatgggat tattgccact gttaacgttg
     1981 ggctagtccc tatcggtatt gctgtagcag gggataactc caaactgtat gttgctaatt
     2041 ccggtacttc tacagtatct gtcatagata tagcaactaa tcaagttatt aatacagtgg
     2101 aagtagagcc taacccaatc gctgttttta tagcgccaga taattctagg gtctatgtag
     2161 caaatgcgga taccattaca gtaatagatg taccgcgtga tatagttata aacaaaatct
     2221 ttgtaagagg tggtggacct accaatatag caatagcgcc ggatggctca aaggtctatc
     2281 tagctagctc aggaagggat agagtatctg taattgatgt atcagttgat cgtattattg
     2341 ctacaatcaa tgtaggttcg aaacctagta gtatggctat aacacctgat ggctctagtc
     2401 tttatgttac taatttctca agcaatacag tatcagtaat aaatatatca actaacgaag
     2461 ttattgatac gttgaatgta ggaaacgccc ccctcgcaat tgcaattgcc ataacccccg
     2521 atggggctaa aaaagcttat gttgccaatt tctcaagtaa cacagtatca gtaattgatg
     2581 tatcaactaa ctcagttatt ccaacagtga tagatgttgg tctcggaccg agctttatag
     2641 ctataacccc agacggtaca agggcatatg taactaacgt cggtaatgac aatcaatctg
     2701 acagtactgt gtcggtgatt gatactacta ctgatacagt cgcagctaca gtgggagtag
     2761 gatcaagacc tgttgatctg gctattactc cggatagcac cagggtcttc gtgcccaatt
     2821 ttctaagtaa tacgctgtca gtaatagatg cactgactaa tttagttgta actacagtga
     2881 ctgatgttgg ccctgggccg agctttatat ctttaacccc accccctgat agctttaagg
     2941 catatgtagt taatccggct gaccagtcct catttggcga aactggaaac actgtatcag
     3001 tagttgatat aataaataat acgctcatcg ccgctgtgca ggttggatct aggcctattg
     3061 atttagctat aagaccggac gggtctcaag tttttgttgt aaactacttc agtaacactg
     3121 tctcggtaat agatacgttg acaaatctgg ttactgctac gttgaatgtt ggaaatgccc
     3181 ctctcgcaat tggaattgcc ataacccctg atggagctaa ggcttacgtt gctaacaccg
     3241 ttagtgagac tgtatcagtt attaatgtat taacggatga ggttattaaa acaataactg
     3301 ttagccctag gccttctgta taaatacgaa tatgatgatt attgatttta tatgtgatcg
     3361 aatgaagatt ctcaattata tctcggttat tttgcttatt tgctcattat tgactgcttg
     3421 tatcggtgta aagcagagtg atattaaaat taaggatgaa aaggcagtcc agctagtcaa
     3481 agaagcgata aacttatacg ataaagaaaa gtatgatgga gcattagatt tactctcaga
     3541 agcaaaaaat caagccagac tgccggagga taaattagaa attgcagata tactttccaa
     3601 aggcggtttt gggctcctta agaaaaagga attcaacaca gcacttgcat attatgaaaa
     3661 ctctttagat ataaatagga ccttagattt taagcccgga ctaattaata actatagtta
     3721 tattggaata atttataccg acctcggaag atacgaggat ggaatcggat attttaagaa
     3781 agccttagag atacaagagg aattaaatga taaatctgga attgctcaaa acctaaataa
     3841 cgtcgctaac ctctatagct atctaggcaa ttatcaggaa tcaataaagc ttttaaaaca
     3901 ggcgcttgga cttagtgagg agattaatga ttctacacaa acagcaaaga cattgattaa
     3961 tctagctaca ataaatctcc gcctaaggaa ttatcaaaac tccatccaat atctaaacag
     4021 cgccttggat attgcgggta gtgcaaaaga agaaaatctc aaggcatact cacttaattt
     4081 gacaggggct atttatagac aacaaggtga ttacacaaag gcgttgcatt actacgagga
     4141 cgctttaaag ataaataaaa agttaggtct tcagactgaa atcgctacca atctaagcat
     4201 tattggcgag ttatataaag agttgggtaa gtatgatgat gcccttaaat attttcagca
     4261 atcgcttggc ataagcgagt catctaagga cagattacta accgcaataa acctaaattt
     4321 cataggagag gtaaaatata aacaggggaa ttatgacgaa gctcttggtc tttataattc
     4381 ttccctggaa atatttgagg aactcggttt taaagacaga attgctcgaa gtttcaatga
     4441 tataggctac ctgcaaggtg aaaagaagaa gttagataat gcaattgaaa accttgataa
     4501 agctatcaca atctataaag acctaggaga tcgagagtgg gttcgtgttg cattattcgg
     4561 aaggggggtt tattcagagg aaaaggggga tttagtttct gccgaaataa actacaaaga
     4621 agcggtagag gtttttgagt caataagaga tgacgtctca ggggatgaag aggctaagca
     4681 gctcttcagc gaggttaata ttaaggttta cgaaaatttg gtttctctgt taataagaca
     4741 gggtaaaaaa gaagaagccc ttgagtatat cgaaagaagc aggtctaaaa ctctccgtga
     4801 tacatttctt aagagcggca taacctcatc tgatgaaaaa actagaggat tattgaccct
     4861 atatgatgaa ctttctcgaa aagaggcttc tataaattac gaacttgtaa atgaaaaagg
     4921 aaaacccaaa cctaatttgg acaaggttgg taatcttgta aagacactcg caaaaaccag
     4981 aaaggatttc agtcaggtta ctaatgagtt gagtactgaa cacgcagacg tatataaact
     5041 tctcagcata agacccggga cattttttga cttagaaaag aaaaataaac ttcccagcga
     5101 tactgttttt attgagtatt ttatcacgga aaaagagacc tatatattcc taatgggcga
     5161 gagggattta atagtaaaac gtgtttcggt gacaaaagaa gaattaaatg aactggtttc
     5221 tctctttaga gatattatac aaaagaacaa atctattcca tctaaggcat ggcgggacgg
     5281 cggaactgat aaatatactg gcaatataaa aacttttaag gatgtgtcaa tgagattata
     5341 tcagtatctc ctggagccga ttgagaatga aacaagacag gcagatattg tagcaataat
     5401 cccatttggg tctcttcatt atcttccctt tcatgcatta gctaaggaag attcagatgg
     5461 gggcatagag ttttttatag agaaaaaggg gttggtttat cttgtatcta catctgctaa
     5521 ttatctagat ctagtactta gagacggtaa aaagagagaa attggtagcg ttgttgcttt
     5581 tggtaatcct gaccttgggg aacctgagct tgtgcttcct tattcagagg aggaggtctt
     5641 agtaataaaa gagatgtttc ctaatgcgac tgtcttttta aagggggatg ctaccaaaga
     5701 taatttcaag aggagttggg gaaggcatga aatagttcac atggcagcgc atggacttat
     5761 tcaggatgag ccatcaattt tattagcacc gctagggtct ggcagtctta ccctctcgga
     5821 tatcacggga ttacaaccgg tgagaaatac acaactagta gtgctttctt tgtgtgatgc
     5881 ggccatagag ggcaatggaa gtaatccgac agggggggaa ttaaacagtg tagcgcttgc
     5941 ttttagctct gttggtgcat cttctgtaat tgctagtctt tggagggtgg atgacagggc
     6001 tacatcacag ttgatggaga aattttatag gaatctaaaa atcgagggga aatttagcta
     6061 tgaggcttta agaaaggcgc agcttgatat gctcagaagg tctgacaagt acggtcaacc
     6121 tttttattgg gccccctttg tattagtcgg tgtatgggag taaatggatt tcaaaagaat
     6181 gaatatttct ttttatcaat ataaaggaac tagtcactta actggcattt tatggagagg
     6241 agcgctgagg caatgtctca atcgcttttt aaattattca ctgacactag aaatggaaat
     6301 acacggagcg agtgtaagta aaaatggcaa attccatgaa cttttcgtct aatcttagac
     6361 atgataggat tgaaggatat gaagtttgaa aaagagaatg aaatccttgg agcagtggca
     6421 acctccttga ttgaaagaaa ggaaggtatg tgtctgactg acgaggagtt tgcgctttat
     6481 atagagggaa ggctaagtaa tgacaaaaga aaggccatga tctcacactt tgtttcatgc
     6541 agggattgcc gcgagaggct taccattcca atacctcatt ttgaggaagc aatagaacca
     6601 aattctatag aaagattgct agcgccgtta tggcgtcctc ttattgtagc tcctatcgca
     6661 gcggtagtta tcgctctctt aactttcaca ctgtttgttt atcaaaaacc tgaggatatt
     6721 actggagaga gacttagagg agctaattta gtagctttaa aacagttaga tataacttct
     6781 aatctattaa taataatcaa ggagggagat gaacaggaat ttaaaaatga gctcattaaa
     6841 gagctgcccc cagacgttaa ggtatcacgc ctaataatag aggatattaa gcaactcaaa
     6901 ggctctaagg agggagacag gataatttta atcctttata acgatggttc tttaaaggta
     6961 aagcgtgggg aatgaattaa gactttctaa ttatacaagt aaatctgtcc ttacaggcgt
     7021 tcaaatatat catttatgtc ttcgtcagtt ataccctttt ccttaatttg ttttcttaag
     7081 ttctttagta tcctttctat ttttttataa acacccttgt ctttaattcc cgggatcgca
     7141 taagttattt cctttgcagt taggttttgc tcaaagtaaa gctgtaggat aagtgcgtct
     7201 tctgcctcga gtttttgaac ctcttccttt agggctttta aaaccttctg tcgtttttct
     7261 tcagcttctg ccattattat attctgctcc ggattcgctc caatatattc tgattggaga
     7321 gcattaacat cctccccaag cattactagc tgtatttcct tagttgtctc tagtcttagt
     7381 ctactttccg ttatcctctt ctcaacctta tcaactctgc tttgtgcctc attttgagag
     7441 attttaaatt ctgctctgat ttcattctct acccatgtgc gtgttgagct ttggtaaaaa
     7501 aaaagcttaa agatatgacg attaatttca tcaaagaccc agaagaattt aggtaaaact
     7561 ttatatccaa actgagagcg tctgaaatca attatgagct tgtttgtaac actgtagaga
     7621 aatgtccgga attttgattc tcctttaaaa gccttcaatc tgcgtgaatc atcctcgttg
     7681 agcttctccc atacatataa gaagcagtcg ttagcaaagt ccttatttct ttgaaaacta
     7741 cgtgtacaga tatctctaac aaaaagaata tcttcttctg ataagatttt tttatccatc
     7801 ttttcgtgaa tcgtggcttg tggttcgtga attgtgattg gtgaatcgaa tatcgtgtat
     7861 tgaactacga gtcacttccc tacgaattta tcggagcacg aatcacgaac cacgtattag
     7921 cagagatttt aatactttat ttgtttaatt aaaagggaga atatctaaaa acttttcgtc
     7981 taatcaaaac aaagaattaa tcattttttt gattgtgcaa caattaattc atagagaaag
     8041 aatggttatt atttgataag taatcggatc ccatcagaag cgacaatgaa aattcaaaaa
     8101 atttttttag cttctatata aattaacggt gcaatcctca tgggtcggtc attttgaaaa
     8161 gcctaaaaga ttttttattg atggaagatt agaggggaaa gtgtcaaaac atttcgtcta
     8221 acttaagatg attttgtttc atagctaaac tcaaccaact ttattgactt ttatatttag
     8281 gttttatgag atctaaatga acaaataagt taatagtttc ataggtgaga gaatgaacag
     8341 tttaaaccag agagttagat cctcggattc tcaatttaaa tctttttcaa atcctatttc
     8401 gcatactccc cgaaacacaa ataagcaaag aatatcaaat ctggatcaaa agcttataca
     8461 aacctacttc aaggaggttg ggtccgagcc acttctcaca gcgacggagg agattaaaat
     8521 tgcaactaaa atgaaaaaat gccaaataag ggctaaagaa ttaaaaacga gtattgaaag
     8581 aagtcttggc ataagcctcg gaaataatat cgagaaaagc atgcatgtgt taaaagaaat
     8641 ttcaggtaac ggctcagata ttcctaagtc agaaaagtcc tcaaaacagc tttcaaggct
     8701 tattaccctc tttaaggctt atctagaaaa aggagctcag ctcagaaata tttttataaa
     8761 atcaaattta aggctagtag taagtatagc aaagggatat acaggttatg gacttccctt
     8821 cttggactta attcaagaag gtaatatcgg actaataaaa gccgtagaaa ggtgtgatta
     8881 tacaaaggga tataagtttt ctacttatgc ctgctggtgg attcatcagt caatatgtag
     8941 ggcgattact gatcagacaa gaactataag ggttccagct tacataattc aaaaatcagg
     9001 taaggttcga gatatttatt cttctttaga ggaaaaaact ggtagacgcc ctttacctga
     9061 agagatttca aaggaagtca acatatcatc agaaggtgta aagcgggtgc ttagggctaa
     9121 tgaaaaggta gtttgtcttg actcgcctgt gtggcagggc gagaaaacaa cccttatgga
     9181 ttctatgcca gatcccaatt ccgaacctcc agattcactg atagcagcag ctagtcttcc
     9241 acaaagtgtg gatgaggcac ttttgatact tagcccaaga gaaagagaga ttcttaagat
     9301 gcgatttgga ataaactatg aaaatcccgt cactctcgat gagattggga aaggtcttga
     9361 tttaaccaga gagagggtca ggcaaataga gaagcgctct cttgaaaaac tccgtaagtc
     9421 tgattttgcc ccagcactga ggagtttaat ggaagtgtgt caataatttt ttgccatttt
     9481 aaactcagcc gctagctcgc tgcatgtata cgaacaaaat cagggcagca agaagaatgc
     9541 ggtaaataac aaaaactgaa tagctacttt tttgcacata tattagaaga tattttatgc
     9601 ttagaaatcc aaaaactgca gaggtaaaaa caccgctaaa aaatataggg tttgcaacgg
     9661 ctgcaatgtc cagatgtctt gattcaagta ttgcagcgcc tgtaataaga ggggtgctta
     9721 gcagaaaaga aaacctagca gcctcctctc ttttaaagtt tctgatcaaa cctcctgtga
     9781 ttgtaattcc agagcgtgag actccagg
//
LOCUS       DATDRL010000068         2616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2006226, whole genome shotgun sequence.
ACCESSION   DATDRL010000068 DATDRL010000000
VERSION     DATDRL010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2006226"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..922)
                     /locus_tag="VI935_02420"
     CDS             complement(146..922)
                     /locus_tag="VI935_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLE24488.1"
                     /translation="MPEVLVKGLKINYIAGEGLEKSRPSILMIHGAGQSGLTWEYQAD
                     ELKKHSKFNLFILDLPGHGRSEGNGLSSIKEYSGFIKDFTNTLALEKLVLVGHSMGGG
                     IAQVFTVNYPDTVYACVLAGTGSRLRVAKETLKVVKNNYDAYCKIAPTRAFASSSPEG
                     LKRRFVEGILRTPQSVVHLDFIACDEFDIMNEVEKIKVPTLIISATEDILTPVRYGEY
                     LHNRIEGSKFHIIKDSGHFMMQEKADEFNRVLLEFLISLS"
     gene            complement(1044..1340)
                     /locus_tag="VI935_02425"
     CDS             complement(1044..1340)
                     /locus_tag="VI935_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HLE24489.1"
                     /translation="MHSSKEESEVTIEVQVQPKSSRDEIVGLQNGRFKVKVAAPPEDG
                     KANERLREIIARALGVSKSGVKIVRGETSRIKILKIKGVNQYILDSFIRRFTQD"
     gene            complement(1341..1649)
                     /locus_tag="VI935_02430"
     CDS             complement(1341..1649)
                     /locus_tag="VI935_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HLE24490.1"
                     /translation="MFVLGNLVGALAIVLHYLLNVYMWIVIIRAIISWVSPDPYNPIV
                     RFLYKVTEPVLGYARRIVPPLGGIDLSPILVLVLIVFLDRFLVETISELAFKLKTGTL
                     "
     gene            complement(1649..2464)
                     /gene="proC"
                     /locus_tag="VI935_02435"
     CDS             complement(1649..2464)
                     /gene="proC"
                     /locus_tag="VI935_02435"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HLE24491.1"
                     /translation="MNKIGFVGAGNMTEALVKGLLTSGVFTRDQIILSDVIQERLHLM
                     SSLYGVKTTLKNKEVVKSSDLIVLALKPNRIGRVISEIKGGLTSKKVLISIAAGITTS
                     YISEAIKKKMKIVRVMPNTPALVLTGASVLYFNSQINQKEKDSIRRIFESVGTVDVVE
                     HEDLLDAVTGLSGSGPAYVAMFIEALSDGGVKMGLTKNMALKLAAQTVYGTAKMVLEG
                     GLHPAEFKDKVLSPGGTTIEGIHELEVGGLRGSVISAVQAAARRSKELSKEGK"
BASE COUNT          764 a          615 c          421 g          816 t
ORIGIN      
        1 atccctatca gaaatgacat ccctatcaga aataagaata acccggtttt attggaaaag
       61 gatttaccaa agagataatt attaaaagct accacacaca ttaaactttg tatatctgct
      121 taaccaagac atgccgcata atcattcaac tgagggagat caagaattca agcaaaaccc
      181 ggttaaactc atctgccttc tcttgcatca taaagtgccc tgaatcttta atgatatgga
      241 attttgaacc ttcgattcta ttgtgcaaat attcaccata tcttactgga gttaatatat
      301 cttcagttgc agaaataatc agcgttggaa cctttatttt ctcaacctca ttcataatat
      361 caaactcatc acatgctata aaatccaggt gtactactga ttgtggggtt ctaagaattc
      421 cctctacaaa ccttctcttt agaccctcgg gcgatgagct tgcaaaagct cttgtcggag
      481 caattttgca gtatgcgtcg tagttgtttt taacgacttt tagggtttcc ttagcaacac
      541 ggagccttga tccggtacct gctagaacac aagcatagac cgtatctggg taattaactg
      601 taaaaacctg tgctatcccg ccgcccatcg agtgccctac aagtacgagt ttctctaaag
      661 caagagtatt agtaaaatcc tttatgaagc cactgtattc tttaatgcta ctcaaaccgt
      721 ttccctcgga cctaccgtgg ccgggaagat ctaagataaa aagattaaac tttgagtgtt
      781 tttttaattc atcagcttga tattcccaag tgagaccgct ttggcccgca ccgtgaatca
      841 ttaaaatact cggacgtgac ttctctaaac cttctcctgc gatatagttt atttttaacc
      901 ctttaactaa gacctctggc actttatcac gcctcttatt aatctttatt atccactaag
      961 aagtcttatc cacctgctct tatttatgca gattcgaacg cttcctagat ttcttggaaa
     1021 agctttcact tgattatcaa atatcaatct tgcgtgaatc ttctaataaa cgaatctaat
     1081 atatactgat ttacaccctt aatttttaaa atctttattc tcgatgtctc gcctctaact
     1141 atcttaacgc ccgatttgga aactccaagc gctcttgcaa tgatttctct tagtctctca
     1201 ttcgctttcc catcttctgg gggagctgca accttcactt tgaatctacc attctgaagt
     1261 ccgactatct catcacgaga tgatttgggc tgaacctgaa cctcgatggt tacctcggat
     1321 tcttcctttg aagaatgcat ctaaagggtt cctgtcttta acttaaatgc aagttcactt
     1381 atggtttcaa ctagaaatcg atctaaaaat acaattagca cgagaactaa aatcggggat
     1441 agatcaattc cacctagggg cggaacaatt ctccttgcat acccaagcac tggctccgtt
     1501 accttataaa gaaaacgcac tatcgggtta tatgggtcag ggcttaccca tgagattata
     1561 gcccttataa tcacaatcca catatagaca ttaagcaaat agtgaagcac tatcgctaag
     1621 gctccaacaa gattcccaag aacaaacatt actttccctc cttggataat tcttttgaac
     1681 gtctagcagc ggcttgaaca gcagagataa cactccccct taagccaccc acctccagtt
     1741 catgaatccc ttcaatcgtt gtaccaccag gagacaaaac cttatcctta aattctgcag
     1801 gatgaagtcc gccttcgagc accattttag cagtgccata aacagtttga gcagcgagtt
     1861 taagggccat attttttgtg agccccatct taactcctcc atcggatagg gcttctataa
     1921 acatagctac ataagccgga ccactaccgc ttaaacctgt aacagcatca agaaggtctt
     1981 cgtgttcgac aacatctaca gtccctacag attcaaaaat tcttctaatg ctatctttct
     2041 ctttttggtt tatttgagag ttgaaatata gaactgaagc tcctgtaagc acaagtgcag
     2101 gtgtattggg catcacacga actattttca ttttcttttt tatcgcttcg gaaatataag
     2161 aagtggttat gcccgccgca atagagataa gaaccttttt agatgttaaa cctcccttta
     2221 tctcacttat aaccctacca atcctatttg gttttagagc gagtacaatc agatcagagg
     2281 atttcacaac ctccttattt ttaagtgtag tcttgactcc ataaagagag gacataagat
     2341 gcaatctctc ttgtatgaca tcactcaata ttatttggtc ccttgtaaat acacctgatg
     2401 taagaagccc ctttacaaga gcctcagtca tgtttcctgc acctacgaaa ccaatcttat
     2461 tcatctcttt ctcccgatga tattatttag ccacagagta cacagagagt agaaataact
     2521 aaaaattatt ttttaatctt tcctttgcgc cctctgtgct ctctgtggca aatgctttat
     2581 tcccttctag gtccaaagat cgctgttcca attcta
//
LOCUS       DATDRL010000069         2995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_858663, whole genome shotgun sequence.
ACCESSION   DATDRL010000069 DATDRL010000000
VERSION     DATDRL010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858663"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VI935_02440"
     CDS             complement(<1..307)
                     /locus_tag="VI935_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24492.1"
                     /translation="MLKASFYREISDERVEEFLQKGYKARYFFPHRIYYLPKCGPDGL
                     KLANRMCGVSDPNKQWEVVLYARCPSIDEFPEELFFDDDIIWHQQQFGKVGHVAFAYL
                     "
     gene            complement(469..669)
                     /locus_tag="VI935_02445"
     CDS             complement(469..669)
                     /locus_tag="VI935_02445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE24493.1"
                     /translation="MVEKYPFFSIIIPTYNRPAQLTNCLYTLTLLDYPRNCFEVIVVN
                     DGGEKMLEAVVDPFHNKIKRSI"
     gene            complement(896..1036)
                     /locus_tag="VI935_02450"
     CDS             complement(896..1036)
                     /locus_tag="VI935_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24494.1"
                     /translation="MKRRHTVTELCHFLGVDAPDDYLNDRASIVIKSPHKADTKHSGI
                     VN"
     gene            complement(1092..1619)
                     /locus_tag="VI935_02455"
     CDS             complement(1092..1619)
                     /locus_tag="VI935_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008186670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLE24495.1"
                     /translation="MLEIGDNFYTQKFGGDCITTSDVLHMVENNPKATIVADLTRADH
                     IPSDSFDCIICTQTLQMIYNVQAALQHLYRILRPGGVLLATSHGISKIGRREGIDPWG
                     EYWRLTAQSARRLFEEAFPAANITITTYGNVLTAIASLHGLSAEELCQEELDYCDTDY
                     EVLITIRAVKPEAKL"
     gene            complement(1894..2637)
                     /gene="cmoA"
                     /locus_tag="VI935_02460"
     CDS             complement(1894..2637)
                     /gene="cmoA"
                     /locus_tag="VI935_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HLE24496.1"
                     /translation="MKRDQVFQEGTARASDFVFNTAVAEVFDDMITRSVPFYLEQQSM
                     IKEIAKKFWIPGTNIYDLGCSTATTLINLCGEMKNTPYFIGYDNSIPMLERAKQKINE
                     KGFENRIELRYGDLNGDLSNLSLENASIVTMSWTLQFVRPLGRDNLIRQIYKGLVEGG
                     VLVVTEKVLTNNTHMNRFFIDFYYNFKKRNGYTENEILRKRESLENVLIPYRIDENLT
                     LFRLNGFEIVETFFQWYNFAGFLCVKKPT"
     gene            complement(2682..>2995)
                     /locus_tag="VI935_02465"
     CDS             complement(2682..>2995)
                     /locus_tag="VI935_02465"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24497.1"
                     /translation="GDGKIGQKLIPLKFLGQTEFFSTGMISLSKISGAPILPMFCTVE
                     KNGKTDLVIEHPIKVEENGDREHGLEIGVAKYAALLESYIRQYPEQYRNWHILGKLPK
                     N"
BASE COUNT          805 a          669 c          604 g          917 t
ORIGIN      
        1 ccagataggc aaatgctaca tgtccaacct tgccaaattg ctgctgatgc catattatgt
       61 catcatcaaa aaataattct tcaggaaact catctattga tggacaccta gcatataaca
      121 caacttccca ctgtttatta ggatcactta ctccacacat acgattagcc aatttaagcc
      181 catctgggcc acatttgggt aggtaatata tcctgtgagg aaagaaatac ctcgctttgt
      241 aacccttttg aaggaattcc tctactcttt catcagaaat ttctctataa aatgatgctt
      301 tcaacatatt agttattcgt tctttgaata tgcctatcta acatctatga agtttccttg
      361 tttctcaata gaacataagt gctacctaaa atcattcaac tgatacaggc ggctctgatt
      421 agtttagggt taattacagc gtcttgttat ttaagtgcac tagggcactt aaatacttct
      481 cttgatctta ttatggaatg gatcaactac agcttcaagc attttttccc cgccatcgtt
      541 tactacgatc acctcaaagc agttgcgtgg atagtccaga agggtcaagg tataaagaca
      601 gttcgttagt tgagctgggc ggttgtaagt cggaataata attgagaaga atggatattt
      661 ttctaccatt agaattcatc tgagtttttc ttagacgttg ctagcaagtt aagcatcgag
      721 cgaattcaaa caatcgcata gaaagaacag gaaaatgctg taagtgatca gatcgcttca
      781 gtttgagaaa gggcaacagg cctacactaa tcctgagaac agacaatata ttaatcctca
      841 taggaatagc cgtgcaagaa gggaaactcg gtaatttcct tccttacaat ctctatcaat
      901 tcactattcc actgtgtttc gtatcggctt tatgtggaga tttaatgacg atactagcgc
      961 gatcgtttag ataatcatca ggtgcatcta ctccaaggaa atggcagagt tccgttactg
     1021 tatgccttct tttcatattc taagtccata gctgctagag taatgatagt tgtgttcaag
     1081 actcacaaca gtcataactt tgcctctggt tttactgctc tgatcgtgat taaaacctca
     1141 taatcggtgt cgcaataatc cagctcttct tgacacagtt cctctgctga aagcccgtgc
     1201 agagaggcaa tcgccgttag gacattccca tacgttgtaa tcgtaatatt tgccgctgga
     1261 aaggcttcct cgaagagccg ccttgccgat tgggccgtca aacgccagta ctcgccccag
     1321 ggatctatac cctcgcggcg gccaatctta ctgataccat gagatgttgc caaaagcacc
     1381 ccacccggcc ttagtatgcg atagaggtgt tgtaatgctg cttgaacgtt gtaaatcatc
     1441 tgaagcgttt gggtacagat aatacagtca aaggagtccg atggaatatg gtctgcacgg
     1501 gttaaatccg ccacaatagt tgccttcgga ttgttttcta ccatatgaag tacatcactc
     1561 gtggtgatgc aatcgcctcc aaacttttgc gtgtagaaat tgtctcctat ctccagcacc
     1621 cgcccctgga tatctgaggc gtgttcggat aaaaactgtt cgatgtagta gcgatcaata
     1681 ggtaagccac ggttcaagcc gaagatgcgg ctgatgggcg tcactcgcca aagggttttt
     1741 tctctgaccg ttcctactct tggccactgc aatctgtata gtcgctgctt tgtccgcagc
     1801 cagcggcgaa gatcttctgg gaacaacgct gtcgttatta atctaatcca gtcgttgatt
     1861 gcgtatacta tcccctcatt attaaggtca aaattaggtg ggtttcttaa cgcataggaa
     1921 tccggcaaag ttgtaccatt ggaaaaaggt ctcgacaatc tcgaagccat ttagtcgaaa
     1981 tagcgtaaga ttttcatcaa tgcgataagg gataagcaca ttctcaagcg attcccgctt
     2041 tcgtaagatt tcattttcag tgtagccgtt tcgctttttg aaattataat aaaagtctat
     2101 gaagaaacga ttcatgtgtg tgttatttgt aaggaccttt tcggtaacta ccagcacgcc
     2161 accttcgaca agccccttat aaatctgcct aatcaggtta tctcttccta gaggacgtac
     2221 gaactgcagt gtccaactca tcgtgacaat gctagcattt tcgagggaca gattcgagag
     2281 atctccattc aggtcgccgt atcgtagctc gatacgattt tcaaatccct tttcgttgat
     2341 cttttgcttt gcacgctcta gcatcgggat ggagttatca tatccgataa agtacggcgt
     2401 attcttcatt tctccgcata aattgatcag agtagtggct gtagaacatc ctaggtcata
     2461 gatattggtg ccaggaatcc aaaacttctt tgcgatctct ttaatcatgg attgttgttc
     2521 aaggtaaaac ggtacactgc gggtaatcat gtcatcgaac acttcggcga ctgcggtgtt
     2581 aaatacaaag tcgctcgccc gcgctgtacc ttcttgaaag acttgatctc gtttcattct
     2641 cgcacctcct aatttgtctc aattccctat ggacggtgtg ttcaattctt tggtaacttt
     2701 cctaatatgt gccaatttcg gtactgttcg gggtattgtc taatgtatga ttccaaaagg
     2761 gccgcgtatt tagcaacacc tatttctaac ccatgttctc tatccccatt ctcttccact
     2821 ttgatagggt gctcgatgac caaatctgtt ttaccattct tctcaaccgt gcaaaacatg
     2881 ggcagaatag gtgcacctga tatttttgac agacttatca ttcccgttga aaagaactcg
     2941 gtttgaccaa ggaattttag aggaattaat ttttgtccta tcttaccgtc gccgg
//
LOCUS       DATDRL010000070         5212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3449197, whole genome shotgun sequence.
ACCESSION   DATDRL010000070 DATDRL010000000
VERSION     DATDRL010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3449197"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..756)
                     /locus_tag="VI935_02470"
     CDS             complement(10..756)
                     /locus_tag="VI935_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLE24498.1"
                     /translation="MIEKLTKNKAGKPRKKKVVQPYDPTVPVKVYMNSNPVCVSPETG
                     VKTAFKLLQAKGFKQLLVTDKGKLVGIVTDRDFRKPQFGDKIDTWQEYYRISDLYKVR
                     DIKTSNVVTINENTPLIEAVRIFEKKKFNALPVFSDKGKLVGILSLHDILSSLISSYK
                     GAGRIQEQAFSFLSKLYSLSKDVQSKQFNMYELGKKLGYNATETEYIVATLSRTELLR
                     YEKSSDKVTITPYGIMMAKGEIKVGYAPIL"
     gene            1317..1781
                     /locus_tag="VI935_02475"
     CDS             1317..1781
                     /locus_tag="VI935_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HLE24499.1"
                     /translation="MSPRFVVHEHEATHLHYDFRLEMHGVLRSWAIPKGPSMDDSQKR
                     LAVLVDDHPLEYIDFEGIIPEGSYGAGAVVVWDNGTYDLLEEKTDKISFILKGKKLKG
                     NFTLVRMKGKEKEWLLIKQKDKHSQAGWKLVTSLTAKKRAELNIRIPPCETS"
     gene            2127..2273
                     /locus_tag="VI935_02480"
     CDS             2127..2273
                     /locus_tag="VI935_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24500.1"
                     /translation="MARFVLITWKGRKALEFQAKSRVKNACFPWKWTIGKKVLVDTRI
                     YFVI"
     gene            2450..2836
                     /locus_tag="VI935_02485"
     CDS             2450..2836
                     /locus_tag="VI935_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HLE24501.1"
                     /translation="MANQNQPSLYERLGGVYSIATVVDDFINRIMVDPRLNANPRVDE
                     AHHRVSPAGFKYLVTEMVCWAACGPQQYTGRSMRDSHQHLLISAAEWEAFLDDFQQTL
                     DKFRVPEAEQAELKAIVASTRADIVV"
     gene            3018..3563
                     /locus_tag="VI935_02490"
     CDS             3018..3563
                     /locus_tag="VI935_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009768371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HLE24502.1"
                     /translation="MYGLVNQAIEDLVRSNHGDATWEVIKQRAEVETEAFLGMEEYPD
                     DVTYRLVQAAAEVLNTPSEEFLRAFGEYWVLYTAKKGYGEMLTMAGKNLPEFLQNLDT
                     LHTRVGVIMPNLRPPSFSCTEMSEHSLHLHYYTHREGLAPFVIGLVQGLGKMFGTEAE
                     VHHIKGYHTGEDHDVFYISWK"
     gene            3538..4911
                     /locus_tag="VI935_02495"
     CDS             3538..4911
                     /locus_tag="VI935_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015178133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLE24503.1"
                     /translation="MSSTSHGNSAGFYGNLNLSSQHLRALFPFHIVFDAGMRIIHHGE
                     RLVRLCPTLKAGDLFSEHFQIHRPERIDNFESIFRQLGALYVVRLRSAPTELKGQMVL
                     LQENLILFLCSPLVRRIAEVQALGLSLQDFPLHDPAGDLLFLLQAQHTTIEEAKALNR
                     RLLAEVQKVASLNEVFKKYVPQEFLKLLEKESIVHVQLGDNVQKTMTVLFSDIRSFTT
                     LSESMSPQETFKFINGYLQQMEPMIGKHGGFIDKYMGDAIMALFATAPDHAVKAALDM
                     LHTLDNYNQGRQRAGYIPIRIGIGINTGSLMLGTIGGESRMDSTVISDDVNLASRIED
                     LTKMYGAPLLISEKTFFSLETPLTYSVRVVGRVRVKGKSELTTVFEVFDGDPPEVKEA
                     KRTTLKLFEEAVDLYNRQEFREAHSLFLSCFNKNPHDQTAKIYLERCQEMISAGQQAD
                     WNGITKI"
BASE COUNT         1496 a         1093 c         1126 g         1497 t
ORIGIN      
        1 gatattttcc taaagaatag gggcataccc aacttttatt tcgccttttg ccatcataat
       61 tccatagggg gtaattgtta ctttatcaga agatttctca tatctaagca actctgtgcg
      121 ggaaagagtt gctactatat attcagtttc agttgcatta tatcctagct ttttaccaag
      181 ttcatacata ttaaattgtt tagactggac atctttactc agactatata gcttggaaag
      241 aaagctaaaa gcttgttcct gaattcttcc cgctccttta taggaactta ttaaagaaga
      301 aagaatgtca tgcaaggata atatacctac cagctttcct ttatctgaaa acacgggaag
      361 agcattgaac tttttctttt caaatatccg aactgcttca atcaaagggg tgttctcatt
      421 aatggtgacg acattagatg tttttatatc tcttacttta tacaaatcac taatcctata
      481 atattcttgc catgtatcaa ttttatcccc aaattgcggc ttgcgaaaat ctctgtcagt
      541 tactatgcct actagttttc ccttatccgt tacgagaagt tgcttaaatc ccttggcttg
      601 taaaagcttg aaggctgtct tgactcctgt ttcgggagac acgcatactg ggttactgtt
      661 catatacacc ttcacaggta cagttgggtc gtagggctgt acaaccttct tctttcttgg
      721 tttaccagct ttgtttttgg ttaatttttc tatcattgtt atctatccat cctttcttta
      781 tcttgaacaa cttcagttgt atgaactcct gtagtataat ttttcttatt attatgcacg
      841 aatttagttg tatcacaaaa caactggaaa atgcttttac caaagtcata tctattatca
      901 tccacgaggt ttagcttctt ctgcgtgcag agttatcgaa tgagattttg ttagttgttt
      961 ttatggctta ttagtagctg ttctttttct acctaaccca tagttgaaat tatctcattt
     1021 tatgcataac ccttctctat tcacattatc ttttcaatct tataccttgt caacacgcct
     1081 aaccaaggca agagacaact cttaggttgg tatccagttt ctggaacttc ctatttctgc
     1141 tctttatagt tagttatcca gtgtaaaatt aattttgata atcgatttag tcatgctgag
     1201 cgaaccattg cccaaacact aagattcttc acctccggct cagaatgaca atgtaatgta
     1261 aggtaactat ctgctagttc aggtattatt tagaatataa catactaaag tttaccatga
     1321 gtcctagatt cgttgtacat gaacatgagg caactcacct tcattatgac tttcgtcttg
     1381 aaatgcatgg tgtcctccgc tcatgggcga tccctaaagg accttctatg gatgactctc
     1441 aaaagcggct tgcagtgctt gttgacgatc atccattgga atatattgat tttgagggaa
     1501 ttatcccaga gggaagttat ggagcaggcg cagtggttgt ttgggataat ggaacctatg
     1561 accttcttga agagaaaacg gataaaatta gcttcattct aaaaggaaag aaactaaagg
     1621 gaaattttac attagttcgt atgaagggaa aggaaaaaga atggctcctt atcaaacaaa
     1681 aggacaaaca ttctcaagcc ggttggaagc tagtgaccag ccttacagcg aaaaaaagag
     1741 ctgagctaaa catacgaatc cctccttgtg aaacatcata atcccaaata attcattaag
     1801 gagatggcaa tgattaaaat ttattcttcc tctccttgaa tttgtcagca cctacatata
     1861 taaaccaaga aaaattacaa tatgaggagt cgactaaaaa gtgaaagatg agcttaccgt
     1921 ttcttctcat aagaggtttt tcttgataac tggaaagaag gtgggcggtg gggagattta
     1981 ttattaaagc ttaaagaggg ttggtcttac agagcggaat ataaaggggt agaaggaagg
     2041 cttgagggtt tagcttgtgt tatatagaag ggaggaaatg gtaaggagaa tgcaaaagag
     2101 acaaaataaa agaggaaaat tcttctatgg caaggtttgt tctaataaca tggaaaggga
     2161 ggaaagcact cgaatttcag gctaagagta gggtcaaaaa tgcctgcttc ccatggaaat
     2221 ggacaattgg aaagaaagtt cttgttgaca ctagaatcta ttttgtgata taaatagctt
     2281 gtccataaaa tgggagaatc actagttaga cgggctagtt tcgaatgaag cgtcgtagcc
     2341 gagtagagtt ggcggaggat ctgcgtgtcc acacacccaa tgatgttttc cacaaccgcg
     2401 ccaaacgctc ttccgacttc cgcctaccgc actcaaaagg aggcattcca tggccaacca
     2461 gaaccaacca agtctttatg agcggctggg gggtgtatat agcatcgcga ccgtagtgga
     2521 tgacttcatc aaccggatca tggtcgaccc gcgcttgaat gccaacccgc gagtagacga
     2581 agcccatcat cgggtatcgc ccgcggggtt caagtacctc gtgacggaaa tggtctgttg
     2641 ggcggcgtgt ggaccgcaac agtataccgg tcgttccatg cgcgattcac accaacatct
     2701 actgattagc gctgccgagt gggaggcttt tctagatgat ttccagcaga cactggacaa
     2761 gttcagggtg cccgaggcgg agcaggcaga gctgaaagcg attgttgcta gtacccgagc
     2821 agacatcgtc gtctgagagt gggacccgtc taactcaagt tggaccgctt ctcagatgca
     2881 ttcttctgga aagataggca aatgcaggca cgaaaacttt tgtatatgtt taaagtctta
     2941 attacgatgg tatttgtgga atacttaagt ctgtcaactc aattgatttt tcagactcga
     3001 gatagaagga gttcgatatg tacgggcttg tcaatcaagc aattgaagat ctagtgcgct
     3061 ccaatcatgg tgacgctaca tgggaagtca tcaaacaaag ggccgaagta gagacagaag
     3121 cctttctcgg tatggaagag taccctgatg atgtgactta tagactggta caagctgctg
     3181 ctgaagttct caatactccc tcagaagagt tcctccgtgc ttttggcgaa tattgggttc
     3241 tatatactgc gaaaaagggc tatggtgaaa tgttgactat ggccgggaaa aaccttccgg
     3301 aatttctaca gaaccttgac acgcttcaca cccgagtcgg tgtgattatg ccaaatcttc
     3361 gacccccctc attcagttgt acagagatga gtgagcattc cttacatttg cactattata
     3421 cgcaccgaga aggattagct cctttcgtca ttgggttggt gcagggacta ggcaaaatgt
     3481 ttggtactga agcagaagtg catcacatta aaggctatca tactggggaa gatcacgatg
     3541 tcttctacat ctcatggaaa tagtgccggt ttttatggaa accttaacct gtcttcgcaa
     3601 catctgcgag cactgttccc ctttcatata gtctttgatg cagggatgcg cattattcat
     3661 catggggaaa gactggtacg cctttgtcct acacttaaag cgggggatct attcagcgaa
     3721 cattttcaaa ttcatcgtcc ggagcgcatc gacaactttg agagtatttt ccgacagctt
     3781 ggcgcgctat atgtagtgcg attgcgctct gcgccgactg aactcaaagg acagatggtc
     3841 cttttgcaag aaaatctcat cctgtttctc tgttcccctc tagtacgcag aatcgccgaa
     3901 gtccaagccc ttggtctgtc gctgcaggac tttcctttgc atgatccagc gggggatttg
     3961 ctcttcctgc tgcaagccca gcacactaca attgaagagg caaaggcgtt aaaccgcaga
     4021 ctgttggccg aagtccaaaa ggttgcttcc ttaaacgaag ttttcaaaaa atacgtaccg
     4081 caagaattcc taaaactcct agagaaagaa agtatcgtcc atgtgcaatt gggggataac
     4141 gtccagaaaa ccatgaccgt ccttttctcc gacatccgct ccttcaccac tctgtcagaa
     4201 agcatgagtc cccaagaaac ctttaaattt atcaatgggt acttgcagca aatggagcca
     4261 atgattggta aacacggtgg ttttatcgac aagtatatgg gagatgccat catggctctc
     4321 tttgctacag ccccagatca tgccgtgaaa gcggcccttg atatgctgca taccttagat
     4381 aactacaatc agggaagaca gagagcaggc tacattccta ttcggattgg gatcggaatc
     4441 aacacgggtt ctttgatgtt aggaactatt ggcggagaaa gtcgcatgga tagcacggtc
     4501 atcagtgatg acgtcaatct agcctcccgt atcgaagatt tgactaaaat gtatggggct
     4561 ccactcctga tcagcgagaa aacctttttc agcttagaga caccgcttac ctattccgtt
     4621 cgtgtcgttg gccgtgtcag agtgaaaggg aagtcggagc tgactacggt gtttgaagtc
     4681 tttgatggag atcctccaga ggtcaaggaa gccaaacgca ccaccctgaa actctttgag
     4741 gaagctgttg acttgtataa tcgccaagag ttccgtgaag cgcacagcct atttttgtca
     4801 tgtttcaaca aaaatcctca cgatcaaaca gcaaaaatct acctggaacg ttgtcaggaa
     4861 atgatcagcg ctggccaaca agcagactgg aatggaatta ccaaaattta aacgaagtaa
     4921 tgtacagcgt tctaaccctt ataaggcgtt gcaaccgacg ccccccgcgc ttattgaaat
     4981 aaaattagat ttgttataga atgaatatta aatgatttgc agtataacac cactaatagg
     5041 gaattatacc gcatttttgc agtctaatta ttagttctat gaaaggggat gtcaagagaa
     5101 aaacctctac cgacctggct acagttgaaa aatttcttga cagccagccc cgttgcttta
     5161 gcccgaagcc tgactccctt gtctgatcta gagcttccat cactcaccac tc
//
LOCUS       DATDRL010000071         6476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2708795, whole genome shotgun sequence.
ACCESSION   DATDRL010000071 DATDRL010000000
VERSION     DATDRL010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2708795"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /gene="arsM"
                     /locus_tag="VI935_02500"
     CDS             <1..612
                     /gene="arsM"
                     /locus_tag="VI935_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HLE24504.1"
                     /translation="GYSQEEMDKAPSGSNLGLGCGNPVAIASLKPGEVVVDLGSGAGF
                     DAFLASPRVGKKGKVIGIDMTDEMLEKARENAKKGGFTNAEFKQGDIEALPLEDNLAD
                     VVISNCVINLAPDKKNVFGEIYRVLKPGGRMMVSDTVLTKPLPERIKKDEKMLSACVS
                     GAIMKDEYLKLIEQTGFKDMNIHKEIPGFLEGYSLSITYSAVK"
     gene            1165..1632
                     /locus_tag="VI935_02505"
     CDS             1165..1632
                     /locus_tag="VI935_02505"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HLE24505.1"
                     /translation="MSLIEDKKYLKLAVEQAKKSVEEGGFPAGSVVVKNDEIISEGVS
                     LGRKLNDPTSHAETSSIRKACKKLETRDLSGATLYASLEPCLMCFSVSNWSGISRIVF
                     GCQKTAEMVEKVYYEGSNNLVQINGQNSRKIELVLISDFEQEMLELIKDWERN"
     gene            1731..2546
                     /locus_tag="VI935_02510"
     CDS             1731..2546
                     /locus_tag="VI935_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24506.1"
                     /translation="MMMATVLDKVTRLPGWVYLQMVRRVESQMGYKVVLNSQKPNWID
                     GAMIFDMTPVWKKHGIDAKGVNYYTVGAVKDGLSSRYDYLSPYYQAWLGGYVVKFKKN
                     REWTAYDHFHLGEADQLNWLEMYGDKEPLASILQKDFKLVEKINISGFPGILYEGGGW
                     SHSDVGKSGRGFILSGMMAACANMFNMLNKNLDLVGENFIPQWNVNYSTNSYHKVNLW
                     GYVAILELDAKTKAVLYANATRFEDRNGKEYDYFIKIGKDIKRVLLSTRIEKV"
     gene            complement(2680..2898)
                     /locus_tag="VI935_02515"
     CDS             complement(2680..2898)
                     /locus_tag="VI935_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24507.1"
                     /translation="MDLKNDSYEIYIGTKNFTEKYYRDEKGWLKVSARGKKFRMTAEQ
                     VLNHLLPAFAGLKNNLIATVKHKEKREE"
     gene            2927..3376
                     /locus_tag="VI935_02520"
     CDS             2927..3376
                     /locus_tag="VI935_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24508.1"
                     /translation="MASITKKAVKNITPVSKTFTDEERAAMQERARELKAGKVDGEKA
                     VLAKIAEMPEPDRAMAKRLHALVKENAPTLLPKTWYGMPAYANKDGKVVCFFKSADKF
                     KSRYATFGFEEAANLDEGNMWLTSFALKKLTPAEEAKIAMLVAKAVS"
     gene            3446..3631
                     /locus_tag="VI935_02525"
     CDS             3446..3631
                     /locus_tag="VI935_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24509.1"
                     /translation="MNAMFKECMQPHALVHMVSGAAIVCLLLYFVPSLTANLLVLGVI
                     LFVVAFILEFFVNPARK"
     gene            3930..4235
                     /locus_tag="VI935_02530"
     CDS             3930..4235
                     /locus_tag="VI935_02530"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HLE24510.1"
                     /translation="MFITITASKVTPEQHKQIEDFLKGFLPRMKQQPGVVAIYHYARP
                     DKGDESTIVIWESPAAVKEYRESDLIKEVIAYEQKLGLQSVTTREGYPLIFSANQKE"
     gene            4385..4594
                     /locus_tag="VI935_02535"
     CDS             4385..4594
                     /locus_tag="VI935_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24511.1"
                     /translation="MFGGIAESKKAEELKLLEKALTTGDVWHGLVFKKTEDKRVKFEG
                     RLMIELTLFLAGDLKKLFHEQLRSV"
     gene            complement(4648..4992)
                     /locus_tag="VI935_02540"
     CDS             complement(4648..4992)
                     /locus_tag="VI935_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24512.1"
                     /translation="MENAFLILSNWWGLSDKPGSQKMFINADGSYVIKVEFSHPRDQV
                     ENSQKEGNIDKERLKQILEFAEINITQPTNHMIYDAGWAIEYIKESKAIKVVNDLDLW
                     KRLIELVPEYKI"
     gene            5011..5511
                     /locus_tag="VI935_02545"
                     /pseudo
     CDS             5011..5511
                     /locus_tag="VI935_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004475888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
     gene            6014..>6476
                     /locus_tag="VI935_02550"
     CDS             6014..>6476
                     /locus_tag="VI935_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HLE24513.1"
                     /translation="MSKTKVTSGMAMSLDGFTAGTNQSFEKPFGDNFDSDLLDRWMFA
                     EPEKHKHKKEIDAILVAGAFIMGSNMFGPKDRKYNPEWKGWWGDNPPYHAPVFVLSHK
                     KRQPVKMEGGTTFFFVTDGIESALSKAKEAAGDRNVKIMGGANTVNQYLAAG"
BASE COUNT         1989 a         1075 c         1483 g         1929 t
ORIGIN      
        1 ggttattctc aggaagagat ggacaaggct ccgtcaggtt caaacttagg cttaggttgt
       61 ggaaaccctg tagcaatcgc ctcattgaaa cctggagagg tagtagtaga tttggggtcg
      121 ggggcaggat ttgatgcttt cttagcttcg cccagagtcg gcaagaaagg aaaagtaatc
      181 ggtatcgata tgactgatga aatgctggaa aaggcaagag aaaatgccaa aaagggaggg
      241 tttactaatg cagagtttaa gcagggagat attgaagcgt tacccttgga ggacaatctg
      301 gcagatgtgg tgatttctaa ctgcgtgatt aacctggcac cggataagaa gaatgtattt
      361 ggggaaattt atcgagtctt aaaaccggga gggaggatga tggtttccga tacggtatta
      421 accaaacctc ttccggaaag aatcaaaaag gatgaaaaaa tgctgtctgc ctgtgtgagc
      481 ggagcaatta tgaaagatga gtatctaaaa ctgatagaac agacggggtt taaggatatg
      541 aatatccaca aagaaatccc cggttttttg gaaggatatt cattgagtat tacttattct
      601 gcggttaagt aagctagttt tgtaattgaa atttgagcgt ttgccgtccg gatatataat
      661 ggaattgtgg ctgttgataa acgcattgcg tttctaaaac ccggctggat aaacctcttg
      721 ctgactttac tggtgatgtg tttacccgta ttaagagagc aatacaataa cggacaatac
      781 gttacttact atagaccaat agctgttatc attgattact ttcaagattt ccgacagcct
      841 caccttctgt tggttatggc aatatttatt ttgacagttt actttgttgt atctctagcc
      901 attgttggtg tttcgaaatt tgcattccct ctattaaaga gtcaagggaa ccacttaagg
      961 aatacataag gcaatctgaa ttagattagg ggccgtagtc taagctaaga ggagggaggc
     1021 caaaggtggc tagctcctga ataattttta ttaatctctc catactctcg tttatattga
     1081 aactaggtat tggaaacaag ccatttttag cttcaatttt tgtgtaccct ttcatagact
     1141 ggagaacttt tatataattg gactatgtca ctaatagaag ataaaaaata tctaaagtta
     1201 gcggttgaac aagccaaaaa gtcagtagaa gaaggtggtt ttcctgctgg ttctgtagtt
     1261 gttaagaacg atgaaataat ttccgagggt gttagtttag gtcgcaaatt aaatgacccg
     1321 actagccatg ctgaaacttc atcaattaga aaagcttgta aaaagttaga aacaagagat
     1381 ttaagtggag caacacttta tgcctcactt gaaccctgct taatgtgttt tagtgtttct
     1441 aattggtctg gaatttctag aattgttttt ggatgtcaaa aaacagcgga gatggttgag
     1501 aaagtttatt atgaaggttc aaataatcta gttcaaatca atggacaaaa tagtagaaag
     1561 attgagttgg ttttaatttc tgattttgaa caagaaatgc ttgagttaat aaaagattgg
     1621 gaaagaaatt agtatttatt cgctcaaact agtcctgatt gactccaggg tggggagcaa
     1681 aaagaccatg tacacggttt aatataactt ctgtaagggt tggtataatt atgatgatgg
     1741 caacagtgtt ggataaggta actagattac ctggatgggt ttacttacag atggtaagaa
     1801 gggttgaaag ccaaatggga tataaagtgg tgttgaatag tcagaaaccc aactggatag
     1861 acggagcaat gatttttgac atgactccgg tttggaaaaa gcatggaata gatgctaaag
     1921 gggtaaatta ctatacggtt ggagcagtaa aggacggact atcttccaga tatgattatt
     1981 tatcgcctta ttatcaagct tggttgggag ggtatgtggt taagtttaag aaaaatcgtg
     2041 aatggacagc ttatgaccat tttcatctgg gagaagcaga ccaattaaac tggcttgaga
     2101 tgtatggtga taaggagccg ttagctagta ttcttcaaaa agattttaag ttggtggaaa
     2161 agataaatat ttctggattc ccggggatat tatacgaagg tggggggtgg tcgcatagtg
     2221 atgtgggaaa gagtggaagg gggtttattc tatctggaat gatggcagcc tgtgcaaata
     2281 tgtttaatat gttaaataaa aatctggact tagtggggga aaactttatc ccacaatgga
     2341 atgtaaatta ttctacaaat tcttatcata aagtgaactt atggggttat gtagcaattc
     2401 tagaactcga cgccaagacc aaagcggttt tatatgcaaa tgcaactagg tttgaagata
     2461 ggaatgggaa ggaatacgat tattttatta aaattggtaa agacataaaa agagttctct
     2521 taagcacgag aattgagaag gtttaagggt tggtgattgc agatttcaaa atagcgggta
     2581 gaggtccaaa agaggttagt tcttgcatag ctttaattag tctttcaata ctttcattta
     2641 tattgaaact tgggtttggg agcactaatt tgatcataat tactcttctc gtttctcttt
     2701 atgtttaaca gtagcaatta agttattttt gagacctgca aaagcaggca ataaatgatt
     2761 tagtacttgt tcagcagtca tcctgaattt ctttcctcgt gctgatactt tgagccatcc
     2821 tttttcgtct ctgtagtact tctcagtgaa atttttagtg ccaatataaa tttcatagct
     2881 gtcattcttg agatccatta gtttagattt tagtataatg cattctatgg caagcatcac
     2941 aaagaaagca gtcaagaaca ttaccccggt tagcaagacg ttcactgacg aggaacgagc
     3001 tgcgatgcag gagcgcgccc gagaactgaa ggcgggcaaa gtggacgggg aaaaggccgt
     3061 gcttgcgaag atcgccgaaa tgccggaacc ggatcgtgct atggctaagc ggctccatgc
     3121 tctcgtcaaa gagaacgcac caaccctctt gccgaaaact tggtatggga tgcccgcgta
     3181 tgccaacaag gacggcaaag tcgtctgctt tttcaaaagc gcggataagt ttaagtcgcg
     3241 gtacgcgact tttggctttg aagaagcggc gaatttagat gaaggcaata tgtggctaac
     3301 ctcctttgct ctgaagaagt tgacccccgc cgaagaggct aagatcgcta tgcttgtagc
     3361 aaaagctgtg agttgaagct atttcagttt gattttcttc aagttttctg caagactttt
     3421 atctaaggag gtgattctag tgaatatgaa cgcgatgttt aaagagtgta tgcaaccgca
     3481 tgcactcgtc catatggtat caggggctgc aattgtttgt ttgctcttat attttgtccc
     3541 aagtttaact gctaatctat tagtgcttgg tgttatattg tttgttgtag cctttatttt
     3601 ggagtttttt gtaaacccgg cacgaaaata ataatttaag aggtgattaa ttcaattttc
     3661 acaaatgtcg aattaatatc ggctcaaatt cctcgtctgt ctccagaaaa tacgtcctaa
     3721 gtgcactaac attggggagc taaatgtacc gctaagcttc ttagtataac cttgcgtttt
     3781 tttgatacac ttattatgta tgaaagatgt cttgaaaaat ctttcgaaaa cgaaaaagaa
     3841 tttgccgcca atgaaaaact tgaaattatt gacgcgggct ttaataaaat aaaggtcaaa
     3901 gaattaaaaa gtaaaattaa gaattaaata tgtttatcac aatcacagct agtaaggtta
     3961 ctcccgaaca gcataagcag attgaggatt ttctcaaagg gtttttaccc cgcatgaagc
     4021 aacagcctgg tgtcgttgct atctatcact acgcgcggcc agataaagga gatgaaagca
     4081 ccatcgtcat ctgggaaagc cccgctgccg tgaaagagta tcgggaaagt gatttgatta
     4141 aggaagtgat agcatacgaa caaaaacttg gattacagag tgtgactacc agggagggct
     4201 atccgctcat tttcagcgca aaccaaaaag aataattcca accgaatggt aatagaattt
     4261 gccgccaaag aaaaactgta cttttatcta agttgcgcct actgttgatg agatatcgtc
     4321 ctgtaactct cctttcaata gcttctttat ttcgatccat actttaaatg ttgatacatt
     4381 gtttgtgttt ggcgggatag ccgagagtaa gaaggcagaa gagcttaagt tgttggaaaa
     4441 ggcgctgact acgggagatg tttggcatgg attagtgttc aagaaaactg aagacaaaag
     4501 ggtgaaattt gagggaagac taatgattga gctgacattg tttttggcag gtgatctaaa
     4561 gaagctgttc catgaacaat tgagatcagt ttagctttgg gagtcttact gattttatcc
     4621 gaaccggata atttatacca tcaagcttca gatcttgtat tcgggaacta actcgattag
     4681 tcttttccaa agatctaaat cgttgacaac tttaattgct ttactttctt ttatgtattc
     4741 tatcgcccaa ccggcatcat atatcatgtg gtttgtcggt tgggtaatat ttatttccgc
     4801 aaattccaag atttgtttca atctctcttt gtcgatgttg ccctctttct gagaattctc
     4861 aacctgatca cgtggatggg aaaattctac ttttatcaca taggaaccgt ccgcatttat
     4921 aaacatcttt tgagaaccag gcttatcaga caaaccccac caattgctta aaataagaaa
     4981 ggcattttcc attactaata atatatcaaa attttgtcaa agttaaaagc caactttatt
     5041 agtcctcttc catattgtga tgaaaatata aatcgccata aaggttgcca gtgggctaaa
     5101 gtacgagtaa agctggaagc tgattctccc tgtggtcact caatgaaatg gaaagaactg
     5161 gtggtgaacc ggatgttgta ggtctcggta taaagacagg gcgcctgcct gccggcaggc
     5221 gggaatacat ttattgttca gcggaaagtc caaaagggcg cagaaatgtt tgttatgacc
     5281 gtgaggggct ggagtcaagg aaagggcata aaccactaaa tatcgctatt gacatggcag
     5341 ctgctatggg aatcgagctt ttgactgaag aacaatatcg agagttgcat aaacttggaa
     5401 atttcgatac gaagacgtcg agctgggtga aaacaccttc tgatattaga aaactcggcg
     5461 gcgccctctt actatggcgt tagggcattc cgtggctcac taagggtcta aattttgcac
     5521 agacagctca tttttcttgt actctacttt gattgtgata tttggtttta taccagacag
     5581 agccggagta gaaaaaattc atctgttaat ttcgtctcca ggtggatctg tctttcatgg
     5641 tttatccgtt tcccaaagga gcggacttgt ctgttattgg agaacttagt cagcgaaaac
     5701 agaacgcgga taagagaatc ttgagaaaaa cccacagatt taaaagaggg tgaaattaga
     5761 gtgatgcaac ttgatgaggt ttgattaaga tcatctcccc ttttaacttt tgaataaata
     5821 ttgcatcctc gaatactttg aaaatggatt cgacaggctc accgcaagta cacacaaatt
     5881 ggctggttat attaacatta cgatcctcga gcacatctat ttcagccttg aattagttaa
     5941 agtcggttga tacttaatat aacctctatc aaattcttgc gcgttccaat agaagatttg
     6001 ttaatataac aatatgagca aaacaaaagt tacctctggt atggcaatgt ctcttgatgg
     6061 atttactgca ggcactaatc aaagtttcga aaaaccattt ggagacaatt tcgattcaga
     6121 cttactagat cgatggatgt ttgcagagcc ggaaaagcat aagcataaaa aagaaataga
     6181 cgccattttg gttgctggtg cgttcattat gggtagcaat atgtttgggc ctaaagacag
     6241 aaaatacaat cctgaatgga aagggtggtg gggtgataac cctccgtatc acgctccagt
     6301 tttcgttctc tctcataaga agaggcaacc agtaaaaatg gagggtggaa cgacattctt
     6361 ctttgtgact gacggcattg aatctgctct ctcaaaggca aaggaagcgg caggtgatcg
     6421 aaatgtcaag attatgggcg gtgcgaatac agttaatcaa tatttggcgg ctgggc
//
LOCUS       DATDRL010000072         4038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3684883, whole genome shotgun sequence.
ACCESSION   DATDRL010000072 DATDRL010000000
VERSION     DATDRL010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3684883"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VI935_02555"
     CDS             <1..220
                     /locus_tag="VI935_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24514.1"
                     /translation="VNYGQIMLIAEVETQETINEEEVQEWRELSNLGTQLVLLVPKEL
                     QNAARDICWRNGIASKVKIGFFEVMLSI"
     gene            602..1006
                     /locus_tag="VI935_02560"
     CDS             602..1006
                     /locus_tag="VI935_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24515.1"
                     /translation="MGKYKEAPTGEDCTNVVKENTGLVREKYLNGFKLPLSLWEENLK
                     VLGTQVEQWHKFQEDYINAVIEFYEKFPSWNGDSKATNSHFEYFTAFQKNYVSLVIGA
                     SDRFMKQTLNIIQKNVEHASSLFDSYPNLLRA"
     gene            1271..1492
                     /locus_tag="VI935_02565"
     CDS             1271..1492
                     /locus_tag="VI935_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010621601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HLE24516.1"
                     /translation="MKGSIKKLVRDRGFGFISAEDGREIFFHTTALQGLTFDSLIEGQ
                     SVEFDVEKSPKDSRSKGPRAINVQAEKQN"
     gene            1612..2157
                     /locus_tag="VI935_02570"
     CDS             1612..2157
                     /locus_tag="VI935_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24517.1"
                     /translation="MINKQLRIARREWENIRQSLEMCGDIGDFNPEDLRNSIWFIGQE
                     YEGFISNIIDMDEKFSQCGYGTSDALNVLTKFASVAAEKLGFKEELASAFGMGYGFVR
                     TGLLAYDKLEPRQILFCKMFYPLGVNFKWDFNPSMVKTKLKIVFEKFRSWQNNPQLYT
                     QDFAQSQKMGEIWKGCNEVNQ"
     gene            complement(2270..3199)
                     /gene="fmt"
                     /locus_tag="VI935_02575"
     CDS             complement(2270..3199)
                     /gene="fmt"
                     /locus_tag="VI935_02575"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HLE24518.1"
                     /translation="MRILLMGTPEFAVPSLRALIESGEEVVAVVTQPDKPKGRGLEIV
                     PPPTKILAEKHRIPVLQPAKIKTEEFFIELKKFNPDLICVAAYGKILPKNILDLPTYD
                     CINVHASLLPKYRGAAPINWAIIRGEKVTGITTMKMDEGMDTGDMLLKKELPIDDEDT
                     GETLSEKLSEIGGKLLIETIRLLKEGRLNPIPQDHSQATYAPMLKKEDGKIDWKKSAE
                     EIKNLIRGTLPWPGAYTNLEGKLLKIYKARLAEDEGKPGEIIKSDSDTLRVATGKGAL
                     DILELQIEGAKRLKTEEFLRGRKIKERTILGGD"
     gene            3318..3647
                     /locus_tag="VI935_02580"
     CDS             3318..3647
                     /locus_tag="VI935_02580"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HLE24519.1"
                     /translation="MQLGVRDSRKNPTEAERKLWQHFRLRQLGGYKFRMQQPLGPYIV
                     DFVCLEKKLILEVDGGQHSEQLSYDTERSTWLEAQGFRVLRFWNNEVLKEIEVVKQVI
                     TEALGLV"
     gene            complement(3740..>4038)
                     /locus_tag="VI935_02585"
     CDS             complement(3740..>4038)
                     /locus_tag="VI935_02585"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HLE24520.1"
                     /translation="EPTAFCRIEQSGKLLRVSVKNQGNADAGASKTTVTFSNKALTLD
                     TPAIPAGGSVDLLFKVPANCFSPDCSFRITVDSINQVDELNNEGNNSVDGGCIG"
BASE COUNT         1234 a          726 c          892 g         1186 t
ORIGIN      
        1 tgtaaactac ggtcagatta tgttgatagc agaggtagag acccaggaga ctattaatga
       61 agaagaggtt caagagtgga gagagctatc aaatcttggc actcagcttg tgcttttagt
      121 cccgaaagaa ttgcagaatg ctgcgcgaga tatatgctgg aggaatggaa ttgcatctaa
      181 agtaaagatt ggtttttttg aggtgatgtt aagtatttag aaaatataac taactacata
      241 aattttcata atctgtataa acattccatc atttaattat tccacatcta gccctggtac
      301 taaagttgta gaggattcat aaggctaaaa tgcctctaaa gtcttattga ttcaatgatg
      361 aaatcagata gatttacttc actgccgcga agccaccttc aattaactag agtttaacgt
      421 ggtagaatcg gctcactcaa aatcattata gccaagagga acaacaattt gacaaatgct
      481 ttcaaatgtg tataatgttt tatctctaat attattccta ctatcaatag tgggaataat
      541 tcaataaata ctcccccttc cccagaattt agagacaatt tcgcttacag gagaaccaaa
      601 catgggtaaa tataaagaag caccaacggg agaggactgt actaatgtgg taaaggaaaa
      661 cacaggatta gtgagagaga aatatctaaa tggttttaag ctacctttat ctctttggga
      721 agagaatcta aaggttttag gcactcaggt tgagcagtgg cataagtttc aagaggacta
      781 catcaatgct gtaatagaat tctatgaaaa gtttccaagt tggaatggag actcaaaggc
      841 tacgaatagc cattttgaat actttacagc ttttcagaag aactatgtta gcttggttat
      901 aggtgcttca gataggttta tgaagcaaac tctcaacatc atccagaaaa acgttgagca
      961 tgcttcctcg ctttttgata gttatcccaa cctattaaga gcataagtag ttcatgctta
     1021 tcatcgggat ttatctacca agcctggcag acatctagct attatggtag ggctctaagc
     1081 tttaatccgg ctgacttaaa agtatttctt ggagaagaac gagataaaga gctatttgaa
     1141 gtagaagagg agataaggag agataagaca ggctgataat cagcagatag ttctcgatgg
     1201 gcagttcggt gaaagagaag aaaatacgaa aaagattata taaggacaat aaaaaaagag
     1261 gtgaatagag atgaaaggaa gtataaagaa gttagttaga gaccgagggt ttggatttat
     1321 tagcgcagaa gatggaagag agatattttt ccatactaca gcccttcagg gattgacctt
     1381 tgattcttta atcgaaggtc aatcggtaga gtttgatgtc gagaaaagcc ctaaggactc
     1441 ccggagcaag ggtcctaggg ctataaatgt acaagccgag aagcaaaatt aactgactag
     1501 agatatcaga ggagaagtta atcttcttaa ttgtattctt ctttcatctg agattttgac
     1561 taaaagagga agcgattctc tataaatact aaagcaacta aagggagaaa aatgataaat
     1621 aaacaacttc ggattgcaag aagggagtgg gagaatataa gacaatcact tgagatgtgt
     1681 ggagatatag gagactttaa ccctgaggat ctcaggaatt ctatatggtt tattgggcaa
     1741 gaatatgaag ggtttatatc aaacattatt gacatggatg agaagttttc ccaatgcgga
     1801 tatggtacct cggacgctct gaatgtactt actaagtttg cttctgtggc agcagagaag
     1861 cttgggttta aggaagaact agcctctgct tttggaatgg gctatggttt tgttcggaca
     1921 ggtctgcttg catacgataa gttagagcct aggcagattc ttttttgcaa gatgttctat
     1981 cccttgggag tgaattttaa atgggatttc aatccttcaa tggtgaagac aaaacttaaa
     2041 attgtctttg agaaattcag gagttggcag aataatcctc agctctatac acaggacttt
     2101 gcccaatctc aaaaaatggg agaaatatgg aagggatgta atgaggtaaa tcagtaaagt
     2161 agaaaggttc tgtactttct gaatgaaatt gaaacacgaa aacggaatct ttagataggt
     2221 ttgtaatgac aattatggaa ggttttgcag ataaccataa atcacggtgt taatctcctc
     2281 ccagtatcgt tctctctttg atttttcttc ccctcaaaaa ctcctcagtc tttagtcttt
     2341 tagctccctc tatttgaagc tctaaaatgt caagtgcgcc tttccctgtt gcaactctta
     2401 gagtgtcgga atcggatttg attatttctc caggcttacc ttcatcctca gcaaggcggg
     2461 ctttatatat ttttaggagt tttccctcaa gattcgtata cgctcctggc cagggaaggg
     2521 ttccacgaat caggtttttg atttcttcgg ctgatttttt ccagtctatc tttccatcct
     2581 cttttttaag catcggtgca taagtagctt gtgagtgatc ttgaggaata ggattaagcc
     2641 ttccttcttt aagaagtctt atagtttcaa tcaggagctt gcctcctatc tcggaaagct
     2701 tctctgataa agtttctcct gtgtcttcgt catcaatagg aagttctttt ttaagaagca
     2761 tgtcacccgt atccattccc tcgtccatct tcatcgtagt aattcccgtg accttttctc
     2821 cccttattat tgcccagttg ataggtgcag cgcctctata tttaggtaga agcgatgcgt
     2881 ggacgttaat acaatcgtaa gtcggaagat caaggatatt cttaggcagg attttcccat
     2941 aagcggcaac acagattagg tctggattaa actttttaag ctcgataaaa aactcctctg
     3001 ttttgatctt agctggttgt aaaacgggaa tcctgtgttt ttctgccaga atcttcgtag
     3061 gaggtggaac aatctcaagg cctcgcccct tgggtttatc aggttgtgtt acaaccgcaa
     3121 caacctcttc tcccgattcg attagagctc tcagagaagg cacagcaaat tcaggggtac
     3181 ccataagtaa gattctcatg tagatggatt atacttgtta attcaggaag tattcaacaa
     3241 acaaatttgt accccttccc cccttgtggg ggaagtgtat agactggaca catgggaagg
     3301 tgggatgggg gaaatgaata caactagggg ttagagattc aaggaaaaac ccgactgagg
     3361 ctgaaaggaa gttatggcaa cattttcgcc tacgccagct aggaggatat aagtttcgta
     3421 tgcaacagcc tttaggaccg tacatcgttg attttgtatg cttggaaaag aagcttattc
     3481 ttgaggtgga tggaggacaa cacagcgaac aactctctta tgatacagaa cgtagcacat
     3541 ggctggaagc tcaagggttt cgagtactca gattttggaa taatgaggtt ttgaaagaga
     3601 ttgaggttgt aaaacaggta ataacagaag cattgggttt ggtttaatcc ccccacctta
     3661 aattctcccc cacaaggtgg gaggagtgaa tctataattc agatgtctga gttgctggct
     3721 agcccactca agctttacat tatcctatgc agccgccgtc aacactattg ttgccctcat
     3781 tattcaattc gtctacctgg ttgatggaat caacagtaat cctaaaagag caatcggggc
     3841 tgaagcaatt tgcaggaacc ttgaacagaa gatcaacaga cccgcctgcg ggaatggcag
     3901 gagtatcaag ggtcaacgct ttattactga atgtaaccgt cgtttttgaa gctcctgcgt
     3961 cggcatttcc ctggttctta actgagactc tcaacagttt cccgctttgc tctattctgc
     4021 aaaaggcggt aggctcca
//
LOCUS       DATDRL010000073         3863 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2443647, whole genome shotgun sequence.
ACCESSION   DATDRL010000073 DATDRL010000000
VERSION     DATDRL010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2443647"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VI935_02590"
     CDS             complement(<1..335)
                     /locus_tag="VI935_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24521.1"
                     /translation="MRKKRLILLLLVIIPFLITHCGDSDRNKIEDLLSKRQKAFETKN
                     VNLYMSCISPTYRQEKDGKVIGIEEIKKNFLSNVTLFDQIKVSHYDRNIYNTDKRVNV
                     SQKTKVDVR"
     gene            complement(332..1135)
                     /locus_tag="VI935_02595"
     CDS             complement(332..1135)
                     /locus_tag="VI935_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HLE24522.1"
                     /translation="MRKIGPIVLIPAIIILFACGSKRLDGSAGPEVLYNRAMNELEKK
                     RGFPWVFTGTDYDLVLKLFKEIQLRYTYSPYASLAELRTGDTYFKKGEYEQAAVDYEE
                     FIKRHPGHEETPYATFRLALSHFKRKKSYDRDPTDTREALRWFNIFVDKYPDSPLVGD
                     AKSMINQCRNSLAKREIYLGNFYAKRKNYKAAAERYKVVVERYQVTSEYEEALYLLGK
                     SYYDMGERQIAKDVLTRVIEEFPTAKYHDKAVSLLAKIEKKEKEEAKEG"
     gene            complement(1213..1731)
                     /gene="def"
                     /locus_tag="VI935_02600"
     CDS             complement(1213..1731)
                     /gene="def"
                     /locus_tag="VI935_02600"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HLE24523.1"
                     /translation="MATLPVLKYPHQILRAMSEPVLKVDDEIRRIVNDMIETMYVLPS
                     CVGVAAPQVGYPLRILVIDVSRKIGPKKNHGLLAMINPVLIYAEGEKMTKEGCLSIPD
                     FLGNVKRSRKVIVKGINPEGNEVEVLSHGLEAIAIQHEVDHLNGILFIHRVSSLASDL
                     VRRKIAYKDIKT"
     gene            complement(1774..2322)
                     /locus_tag="VI935_02605"
     CDS             complement(1774..2322)
                     /locus_tag="VI935_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HLE24524.1"
                     /translation="MRRFFAITAVGKDRPGIVAGVSKALYDLGTNIEDSSMTILSGEF
                     AMILIVSTKEEVDASDLESHFKEVTNKLGLFISIKELNENEIQPKKPYSGIPYIISVI
                     GTDKPGIVYKVSELLASKGINITDLNTKVIPGESAPVYTMILEVEVPEYVDAALLEKE
                     FSIIEKEMSIDISIKELEVLEL"
     gene            complement(2433..3491)
                     /gene="ald"
                     /locus_tag="VI935_02610"
     CDS             complement(2433..3491)
                     /gene="ald"
                     /locus_tag="VI935_02610"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HLE24525.1"
                     /translation="MVIGIPKERKIREYRVSSTPQTIRVLVEGSHRVLVEKHAGIGSG
                     ISDEEFKKAGAEIVESEEEVFNTSKLIVKVKEPLSEEFPLIRKDHILFCFLHLAAYPE
                     LARTLQKIGVRAIAFETVELPDGFLPLLAPMSRIAGRLSVQIGIHLLEKPSGGKGVLI
                     SGAPGVRPAKVTVIGGGTVGYNAVLSAYGLGARVVLIDINPKKLEFFHEEFKGRVKTV
                     PSYPELIEKELLDSDLVIGAVLITGVKAPTVITREMISKMDPGSVFVDVAIDQGGCSE
                     TSRPTNLDSPVYNVDGVLHYCVTNIPSVVSRTSTFSLANSILPYIIKIAEGKIEEDPA
                     LIKGINVDKGKLLRMLSY"
     gene            3745..>3863
                     /locus_tag="VI935_02615"
     CDS             3745..>3863
                     /locus_tag="VI935_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HLE24526.1"
                     /translation="MVKFVDEAKIYVKSGRGGKGCVSFRREKFVPRGGPNGGDG"
BASE COUNT         1134 a          821 c          674 g         1234 t
ORIGIN      
        1 aatcttacat caaccttagt tttctgactc acattaaccc ttttatcggt attgtagata
       61 tttctatcat aatgggagac cttaatttgg tcaaaaagag taacgtttga aaggaaattc
      121 ttttttattt cctcaatacc tataactttg ccatcctttt cttgtctata ggttggcgat
      181 atacaagaca tataaagatt gacgtttttt gtctcaaagg ccttttgtct ttttgagagt
      241 aaatcctcaa ttttattcct atctgaatca ccacaatggg taatgagaaa tggaataata
      301 actaaaagga gaaggattaa tcgttttttc ctcatccttc tttagcttct tccttttcct
      361 tcttctcgat ctttgcaagg aggctaactg ccttatcgtg gtattttgcc gttgggaatt
      421 cctctattac gcgagtaagg acatcttttg caatttgtct ctcacccata tcgtagtatg
      481 actttccaag tagataaagc gcttcctcat attcgcttgt aacctggtac cgttctacaa
      541 ccaccttata tcgttcggca gctgctttat agttctttct ttttgcataa aagtttccta
      601 aatatatctc acgtttagca agactgttac ggcattggtt tatcatactt ttagcgtcac
      661 caacaagggg agaatctgga tacttatcga caaagatatt aaaccacctt agagcctctc
      721 ttgtgtctgt agggtctcta tcgtagcttt tcttccgttt aaaatgcgaa agcgctaaac
      781 gaaaggtagc ataaggggtc tcttcatggc ctggatgacg ctttataaac tcttcataat
      841 caaccgctgc ctgttcatac tccccttttt tgaaataagt atcgccagtt cgtagttccg
      901 caagtgaagc ataaggacta taggtatatc taagttggat ttccttaaat agcttaagaa
      961 ctaggtcata atccgtgcca gtaaaaaccc atggaaaacc tcgcttcttt tcaagttcat
     1021 tcatagccct attatataga acttctggcc ccgcgcttcc atcgaggcgc ttggagccac
     1081 aagcaaaaag aataataata gcaggaatca gtactatagg acctattttc ctcattaact
     1141 ttaacaaaat actatttggg taagggcacc ctgtcaagca ttaattttta aaggtctatc
     1201 ctcatgacag ttttaagttt taatgtcttt ataagcaatt tttctcctta ctaaatccga
     1261 tgctaaagag ctcaccctat gaataaagag aattccattt agatgatcaa cctcgtgctg
     1321 aatagctatc gcttcaagac catgagagag gacttcgact tcattaccct cagggttaat
     1381 tccctttact ataaccttcc tcgacctttt tacattccca aggaaatcgg ggatgcttaa
     1441 gcaaccttct ttggtcatct tctccccctc ggcatatatg agaacaggat ttatcatagc
     1501 cagaagccca tgattttttt taggtccgat ttttctagat acatcaataa caaggatcct
     1561 gagtggatat ccaacttgag gtgcagctac accaacacag ctaggcaata catacattgt
     1621 ttcgatcatg tcatttacta ttcgcctaat ctcatcgtca actttcaata ccggttcaga
     1681 catagctctg agtatttgat gcggatattt aagtactgga agagttgcca ttaatcgaaa
     1741 ttatccatta aatagggttg aaagggtcgg cttttaaagc tcaagaactt ctaattcttt
     1801 aatacttata tcgattgaca tttctttctc aattattgag aattcctttt caagaagagc
     1861 ggcatctaca tattcaggta cctctacttc aaggatcatt gtataaacag gtgcactctc
     1921 gccaggtatg acctttgtat tcagatcggt aatatttatc ccctttgaag caagtagttc
     1981 agagacctta taaactatcc ccggcttatc agttcctata acagagatta tatagggaat
     2041 cccagaatag ggcttctttg gttggatttc attttcatta agctctttta tagagataaa
     2101 aagcccaagt ttattcgtaa cctctttaaa atgactctca agatctgaag catcaacctc
     2161 ttccttggtt gaaacaataa gaatcatggc gaattcacca ctcagaatcg tcatacttga
     2221 atcctctata tttgttccga ggtcataaag ggcttttgat acacctgcaa caataccagg
     2281 gcggtcttta cctacagctg ttatagcaaa aaatcgtctc aagttttatt caccttaagt
     2341 tgatttaaaa agagagcatg taatctctta cgtttcgaat tttaaaataa aagcaactta
     2401 gtacacaatc attctaaatt ctaccattct gattaataac ttaacattcg aagcagcttc
     2461 cccttatcta cgtttatgcc ttttattaaa gcgggatctt cttcaatctt accctcagcg
     2521 atttttataa tatacggcaa aattgaatta gccaatgaaa aagtagaagt ccttgagaca
     2581 actgaaggaa tatttgtaac gcagtaatgc aacaccccat caacgttata tacaggggaa
     2641 tcaagattag tcggacgact tgtttcagag catccgcctt gatcaatcgc tacgtctaca
     2701 aacacgctcc cagggtccat ctttgaaatc atctctctcg taataaccgt aggcgccttg
     2761 actccggtga ttaatacagc acctatgact agatcagaat ctagaagctc tttttctatt
     2821 aattcagggt aagatggaac cgtttttact ctacctttaa actcctcgtg gaagaattct
     2881 agcttctttg gatttatatc tatgagcaca acccgagcac caagaccgta tgccgagagg
     2941 acagcgttat atcctacagt tcctcctccg ataaccgtaa ccttagctgg tcttactcct
     3001 ggggcaccgc ttattaaaac cccttttcca ccgcttggtt tctcaaggag gtgaatccca
     3061 atctgtactg agagtcttcc agcaattcta ctcataggag caagaagtgg aagaaatcca
     3121 tcaggaagtt ctacggtttc aaaggcgata gccctaacgc ctattttttg aagtgtgcgc
     3181 gcaagctcag gatatgcagc aagatgcaag aaacaaaaaa gaatgtgatc ctttcgaatt
     3241 aaagggaact cctctgaaag tggttccttt acctttacga taagcttaga tgtgttaaaa
     3301 acctcctctt cactttcaac aatttctgcc cctgcttttt taaactcctc atcgcttatt
     3361 ccgcttccta ttccagcatg cttttctacc agaacacgat gacttccttc aactaaaacc
     3421 cttattgttt gaggagtaga agaaactcta tactcgcgta tctttctttc tttaggaatt
     3481 ccaattacca tcgagagtta gaatagcatg agttttagaa gtctcaacaa ttttgaccgt
     3541 atgaagactg ttgattagta tttatcgtta gccacagaga acaaagagac ttcgacgagt
     3601 tgatctaggc attcgtagct gttattaaag aattttctta tatgccctcg agttgtcttt
     3661 gcttttaaag taaaaagata ggtaagagca gcttccagct gcgaacaaga aataaactca
     3721 acaatcataa taatataact aaagatggtt aaattcgtag atgaagcaaa aatctatgta
     3781 aagtcaggac gtggaggaaa gggatgcgtg agtttcagac gggagaaatt cgtccctcgt
     3841 ggtggtccta atggtggtga tgg
//
LOCUS       DATDRL010000074         7992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2170207, whole genome shotgun sequence.
ACCESSION   DATDRL010000074 DATDRL010000000
VERSION     DATDRL010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2170207"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VI935_02620"
     CDS             <1..1110
                     /locus_tag="VI935_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon-insertion domain-containing protein"
                     /protein_id="HLE24527.1"
                     /translation="LFKVKADFDSVVERTDDMLLQYACFIKSMCDKEHLKEFDRSGLE
                     TVIEYSSRLAGDQNKLSVQFGAISKVISEANYWASLDTDGNYVTRKHVEKAIEERIFR
                     SNMIEEKIQEMIEKNMILVDTQGEVIGQINGLAVYDLGDYAFGRPSRITCETFMGLEG
                     VVNIERKANLSGNIHNKGVLILTGYIGAKYAQNKPLSLSASLCFEQSYEMVEGDSASA
                     AELIALISSLSGVPIKQGFAITGSVNQKGQIQPIGGVNEKIEGFYHVCKAKDLTGEQS
                     VIIPHQNVRNLMLKKEVVEAIREGKFHLYPVEKIDQAIELLTGMEAGERGPDGKFKKG
                     TVNYLVDKRLREFTEEFKKLSKENRRKVGREETEE"
     gene            1192..1773
                     /locus_tag="VI935_02625"
     CDS             1192..1773
                     /locus_tag="VI935_02625"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HLE24528.1"
                     /translation="MLFCRESSLNVSNLSIMLPNGEEGFIQIEPGEAFGRGNQPSTRV
                     CIKVMESIFKERKVEIVLDVGCGTGVLSICAALLGANRVLALDVDPLAVEETRINIKK
                     NGFTNIQVVYDSLKGVHEKFDLVIANLGTTEILNISDELKYKLKDEGFLLLSGIWLDG
                     QKEMVIQRFKELGLCLDEELYEGGWIALSFRLN"
     gene            1789..3153
                     /gene="radA"
                     /locus_tag="VI935_02630"
     CDS             1789..3153
                     /gene="radA"
                     /locus_tag="VI935_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HLE24529.1"
                     /translation="MKKSKVQLQFVCQSCGNSSPRWLGKCPACGEWNSYVEERVVKEK
                     RGLERPSGKASAVNLTQIESDKEDRISTGIGELDRVLGGGFVPGSIVLVGGDPGIGKS
                     TLALEMLNKVGLSKRSVLYVTGEESPEQVKMRAERLGVSSDRLFIFPETSLEYILEEI
                     NKISPKLAVIDSIQTIFTGDFPSAPGSVGQIRECAAKLMQYAKTSGTAIFLIGHVTKE
                     GTIAGPKVLEHLVDTVLYFEGGQEHPYRILRAVKNRFGSTNEIGVFEMQDSGLQEVKN
                     PSEIFLSERPLNASGSVVVPSLEGTRPILVEIQALVSPCNFGVPRRTTIGLDYNRVSL
                     LVAVLEKRAGLHLLGQDIFMNVAGGVSIEEPAIDIGICACVASSFLNKPIESNIVVFG
                     EVGLAGEIRGVSQVELRLKEAEKLGFKKCVLPKINLDRLKRSDNTLSLVGVGSIEKVI
                     EVLF"
     gene            complement(3192..3626)
                     /locus_tag="VI935_02635"
     CDS             complement(3192..3626)
                     /locus_tag="VI935_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24530.1"
                     /translation="MLYIDTSVLLVYTLTQSVEIERFEVTNKFFAKVISGELIAATSF
                     YALHELYLFALGNTPDLNTGYAFGKAALMKILSTPVRILPFVSRRERANLTDKFSGLK
                     DTTDVPHAISAFSSGCDAIVAYDEHFRAISHIISYKTPEDYL"
     gene            3823..4005
                     /locus_tag="VI935_02640"
     CDS             3823..4005
                     /locus_tag="VI935_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24531.1"
                     /translation="MSKRGMEFFNNHAGHQEEADKVGFKKCVLPKINLDRQKRSDNAI
                     SLICAGSIEKVIEVLF"
     gene            4326..5129
                     /gene="murI"
                     /locus_tag="VI935_02645"
     CDS             4326..5129
                     /gene="murI"
                     /locus_tag="VI935_02645"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HLE24532.1"
                     /translation="MNRSESPLGVFDSGIGGLTVVREILRRLPNENIIYLGDTARVPY
                     GTKSSQTVVKYSQSNSKFLISKGIKMLVIACNTASAVALPSLRWDYEIPVMGVIEPGA
                     RKAVRITKTGKVGVIGTPSTIKSNAYKKAVENIAPEIKVFSKACPLFVPLAEEGWIDG
                     KIAELTANKYLEPLKESGIDVLILGCTHYPLLKSTIQKVIGDEVVLVDSAEETALEIE
                     RTLKENGISNESSSYNKRDFYLTDVSETFIEIAGRFLGDSIEKVEQVDI"
     gene            complement(5171..5908)
                     /gene="pgl"
                     /locus_tag="VI935_02650"
     CDS             complement(5171..5908)
                     /gene="pgl"
                     /locus_tag="VI935_02650"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HLE24533.1"
                     /translation="MMKLEIYIFSDRQQLADTLSRDFHRSLETQANAGGSVFVALSGG
                     NTPRIFFERLAVPPYREEIPWKNIHFFWGDERCVPPDDTESNFKMANDAFLSHVALPD
                     NNIHRVMGENPPQSEVHRYEKEIKTHLPLAENELPQFDRVFLGMGNDGHTASLFPGAP
                     TLLEKEAACVIAEHPTSGLKRISLTLPVINNAKRISFLVTGEEKATIVREILKKSPSS
                     LNYPAAKVKPFNGVLEWYLDKTAAKGL"
     gene            complement(5946..7478)
                     /gene="zwf"
                     /locus_tag="VI935_02655"
     CDS             complement(5946..7478)
                     /gene="zwf"
                     /locus_tag="VI935_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HLE24534.1"
                     /translation="MERPDSFILVIFGASGDLTKRKLIPSLFALYEQNLLPDRFAVLG
                     VSRSDLPDTAFRDRMTEGIKLYSEKQPEEKMLNSFCKNIYYQSLDAVNSPDYTKLKTR
                     LIQIDTELKTRGNYIYYLSTSPNLFGTISESLGKQNLEKEGNGPCWKRIIIEKPFGHD
                     LESAKKLNATLLKIFDEKQIYRIDHYLGKETVQNILAFRFTNGIFEPLWNRNYVHHIE
                     VTASEHIGVEDRGEYYDNYGALRDMVQNHLLQIVGIIAMEPPSFFDATAVRNETVKVF
                     QSLRRIKPEEIRKYAVRGQYTESTIRGEKVPGYRNEKRVAPDSRTETFVALKLYIDNW
                     RWGDVPFYIRTGKRLPTRVTEVVIHFKKTPHHLFKQNNICDVSCNELIIRIQPDEGIL
                     LKFGMKMPGAGFQIKDVNMNFHYSDLGDAYIPEAYERLLLDCILGDATLYPRTDAVEA
                     CWRYVDPILMAWREQPDIKIYGYPAGTWGPKEAHELFDNPEEDWRYPCKNLATDGEYC
                     EL"
     gene            complement(7681..>7992)
                     /locus_tag="VI935_02660"
     CDS             complement(7681..>7992)
                     /locus_tag="VI935_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017999234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HLE24535.1"
                     /translation="PGTPLNAIEEVLDIADFIQIMTVNPGFGGQKFIHSQLDKIRRLR
                     RILDERSSYIPIAVDGGVDTTTAPLVVNAGATVLVAGSSVYNSSGTVAENVNALLKSI
                     R"
BASE COUNT         2409 a         1440 c         1777 g         2366 t
ORIGIN      
        1 ctttttaaag taaaagcaga ttttgattct gttgtggaaa gaacagatga catgttgctt
       61 caatatgcct gttttataaa atctatgtgc gataaggaac accttaagga gtttgataga
      121 agcggtcttg aaaccgtaat tgaatactca tccaggcttg cgggggatca gaacaagttg
      181 tctgttcaat ttggtgctat ctcgaaagtt ataagtgaag caaattactg ggcgagttta
      241 gacaccgatg gtaattacgt aacgcgaaag catgtagaaa aggctataga agagaggatt
      301 tttaggtcaa acatgattga ggaaaagatt caagagatga ttgaaaagaa tatgatactt
      361 gtagatacac aaggtgaagt catcggacag attaatggac ttgctgttta cgaccttggt
      421 gattatgcct tcgggaggcc gtcacgtatc acatgcgaga cctttatggg attagaaggt
      481 gtggtgaata tcgaaagaaa ggcaaacctg agcgggaata tccataataa aggtgttctt
      541 attttaactg gctacatcgg agcaaagtac gcacaaaata aacctctaag cctctcagca
      601 agtttatgtt ttgaacagag ctatgagatg gttgaaggtg atagtgcatc tgcagcggag
      661 ctcattgcgc ttatatctag cctttcgggt gttcccatca agcagggttt tgctattaca
      721 ggctctgtaa atcagaaagg acagattcaa cctattggtg gggtgaatga aaagatcgaa
      781 gggttttatc atgtatgcaa agctaaagat ttaactggag agcagagtgt gatcattcct
      841 catcaaaatg taaggaactt gatgcttaaa aaagaggtcg ttgaagctat aagggaaggg
      901 aagtttcatc tttatcctgt ggaaaaaatt gatcaggcaa tagagcttct tacaggaatg
      961 gaagcaggcg agaggggccc cgatgggaaa tttaaaaaag gaacggtgaa ctatcttgtt
     1021 gataagcgac taagagaatt tacggaggaa ttcaaaaagc tatcaaagga aaatagaagg
     1081 aaagtaggta gggaagaaac ggaagagtag tgatttatgg attattcaga agagactaag
     1141 atcctttcaa tccaaaaggt tttaaatcgg ccacaataga ggaggttttc agtgttattt
     1201 tgccgtgaat caagtctcaa cgtatcaaat ctctctataa tgcttccaaa tggcgaagaa
     1261 gggtttattc aaatcgagcc gggagaggcc tttggtagag ggaaccaacc atctacaagg
     1321 gtttgtataa aagttatgga atccatattt aaagaaagga aggtagagat tgtcctagat
     1381 gtagggtgcg gaactggggt gctctcaatt tgcgctgctt tactgggagc aaacagggtt
     1441 ttggcgttag atgttgaccc tttagctgtt gaggagacta gaataaatat taaaaaaaat
     1501 ggatttacaa atattcaggt tgtatatgat tccttgaaag gcgttcatga aaaatttgac
     1561 ctagtgattg ctaatcttgg gacaactgaa attcttaata tcagtgatga gcttaaatac
     1621 aagttaaaag atgaaggttt ccttttactt tctggaattt ggttagatgg gcaaaaagaa
     1681 atggtgatcc aaagattcaa agagctaggt ctctgcttag atgaggaatt atatgagggg
     1741 ggatggattg ctttatcgtt tagattgaat tagataatct atcaaacaat gaaaaaatct
     1801 aaggtacagt tgcaatttgt ttgtcagagc tgtgggaatt cgtcacccag gtggttgggg
     1861 aaatgcccag cttgtggcga gtggaattcg tatgttgaag aaagggtagt gaaagaaaaa
     1921 aggggtctcg aaaggccgtc aggtaaagct agtgcggtca accttactca aatcgagagt
     1981 gataaggaag atagaatctc aacaggaatt ggagaactgg atagggttct tggaggtggg
     2041 tttgttcccg gctctatcgt tcttgtagga ggggatcctg gaattggaaa atcgaccctt
     2101 gcactcgaga tgctaaataa agtaggtctt tctaaaagaa gtgttcttta tgttactggt
     2161 gaagaatccc cagagcaggt aaaaatgagg gccgagcggt taggggttag ctcagacagg
     2221 ctttttattt ttccagagac atcacttgag tacatacttg aggagattaa caaaatttcc
     2281 cccaaactcg ccgtcattga ctcaatccaa accattttca ctggggattt cccatctgct
     2341 cctggaagcg tgggacagat aagagaatgt gctgcaaagc ttatgcagta cgccaagaca
     2401 agcggaacgg ctatatttct tattggccac gtgacaaaag agggtacaat tgcgggaccg
     2461 aaggtacttg agcacttagt agacacagtg ctttattttg aagggggaca ggagcatcct
     2521 tacaggattc ttagagccgt gaaaaataga ttcggctcaa caaatgaaat cggcgtcttc
     2581 gagatgcagg atagtgggct gcaggaggta aaaaaccctt cagagatatt cctttctgag
     2641 cgtcccctaa atgcttcggg ttcagttgtt gttcctagcc ttgagggtac tagaccaata
     2701 ctcgtagaga tacaagctct tgtatcgccc tgtaacttcg gggtgcctag aaggacaacg
     2761 ataggacttg attataaccg tgtctcgctt cttgtcgctg tgcttgaaaa acgggccgga
     2821 ttacatttat taggacagga tattttcatg aacgttgcgg gcggagtaag tattgaggag
     2881 cctgcgattg acattggcat atgtgcttgt gttgcgtcaa gctttttaaa taaaccgatt
     2941 gaatcgaata tagttgtgtt cggtgaagtc ggtcttgcag gtgaaatacg cggagtttca
     3001 caggtcgaac tgagactcaa ggaagcagag aaactgggtt ttaaaaaatg cgttcttcct
     3061 aagataaatc tcgatcggct aaagcgttct gataatactt tatctcttgt tggtgtaggt
     3121 tcgatagaaa aagtaataga ggtgttgttt tagaatttcg agagaaataa aagttaccgc
     3181 tatctccatc tctaaagata atcttccggc gtcttatatg aaatgatatg cgaaatagcc
     3241 cgaaaatgct catcgtaagc tacgatggca tcgcagccag aactaaaagc agaaattgcg
     3301 tgaggaacgt cagtagtatc ttttagcccg ctaaatttgt ccgtaagatt tgctctttct
     3361 cttcgtgaaa caaatggaag aatacgcaca ggtgtggaaa gaatcttcat gagagcggct
     3421 tttccaaaag cataaccagt gttgagatca ggcgtattcc ccagagcgaa taaataaagt
     3481 tcatgaaggg cataaaagga agttgcggca atcaactctc cagagatgac tttggcaaag
     3541 aacttatttg ttacttcaaa gcgttcgatt tcaacagatt gagttagggt atagacaagg
     3601 agaacagagg tatctatgta gagcaactaa ttatctctta actccaacct tttttggagt
     3661 agtttttttg tgaacagtct taatctcctc ttcacttgcc aaaatgcgtt tcaggctctg
     3721 ctttcgagct cgatgaagaa gtttattaat agacttaccc ttaagatact tgccaaattc
     3781 tcttagttgc atgaataaaa aaattacata gaaaccgttg cgatgtcaaa aagaggtatg
     3841 gagtttttca acaaccatgc agggcatcaa gaagaagctg ataaggtcgg ctttaaaaaa
     3901 tgcgttcttc ctaagataaa tctagatcgg cagaagcgct ctgataatgc gatatctctt
     3961 atttgtgcag gttcgattga aaaggtaata gaggtgttgt tttgatagaa tgtttttgaa
     4021 cttataaaca cgtatagttg aatgataact cagtaaatat ttctccttcc ccccttgcgg
     4081 gggaaggctg gagaaagggg ttagggtcag gctttgctta tttgtttttg tcatcaatat
     4141 aataatccaa caagcaaagc ctgaccctag atctttttat aggacgaaaa ataagatgat
     4201 aacaaatatg ttccttgtca tgctaaaata cttccctata agtttgatta tgccactata
     4261 tcaaagaaaa acgcaatacc tatttttatt gacataaaat tctctgcggc taatcttcta
     4321 gacctatgaa tagaagcgaa tccccactag gtgtattcga ttcaggtatt ggcgggctta
     4381 ctgttgtaag agaaatattg agaagacttc ctaatgaaaa cattatttat ttaggcgata
     4441 ctgcaagggt tccctacggt acaaaatcca gccaaaccgt agttaagtat tctcagagca
     4501 atagcaaatt tcttatatca aagggtataa agatgctagt gattgcatgc aatacagcct
     4561 ctgctgtagc gttaccaagc ttaagatggg attatgagat tccggtcatg ggtgtgattg
     4621 agcctggagc gagaaaagcg gtcaggataa caaaaacagg aaaagtaggt gtgataggaa
     4681 caccttcgac aattaaaagt aatgcataca aaaaggcagt ggaaaatatt gcgcctgaaa
     4741 ttaaagtctt ctcgaaagca tgtcctcttt ttgtgccgct tgctgaagaa gggtggattg
     4801 atggaaagat cgctgagctt acggcgaata aatacctaga acctttgaag gaatctggca
     4861 ttgatgttct tatattaggt tgcactcatt atccacttct gaaatccacg attcaaaagg
     4921 ttataggtga cgaagttgta ctagtagatt ccgctgaaga gacggcttta gaaatagaac
     4981 gaactcttaa agaaaacggt atctctaatg aaagttcctc ttataataag agagacttct
     5041 atcttaccga tgtctcggag acctttattg aaattgcagg aaggttttta ggggatagta
     5101 tagaaaaggt tgagcaggta gatatatagc gttatttcta ttaggtaagg aaaaaataat
     5161 acggtttagc ttacagccct tttgcagcag ttttatccag ataccactcc agcaccccat
     5221 taaatggctt aaccttagct gcgggatagt ttaaagagga agggcttttt ttgaggattt
     5281 ccctgacaat cgttgctttt tcctctccag taaccaaaaa tgaaatgcgc tttgcattat
     5341 ttataacggg gagtgtcagg cttattctct tcagaccgct tgtcggatgc tctgcaataa
     5401 cacacgctgc ttctttttcg agtaatgtag gagcaccagg aaataatgag gcagtatgcc
     5461 catcatttcc cattcctaag aaaacccgat caaattgtgg tagttcgttc tcagccaaag
     5521 gtaaatgggt tttaatttct ttttcataac gatgcacttc agattgtggt gggttttctc
     5581 ccataaccct gtgaatattg ttatcaggta aagctacgtg ggataaaaac gcatcattcg
     5641 ccattttgaa attgctctcc gtatcgtctg gaggaacaca tcgctcatct ccccaaaaga
     5701 agtgtatatt tttccatggg atttcttcac ggtatggtgg cactgcaagc ctttcaaaaa
     5761 atattcttgg agtgtttcca cctgagaggg ctacaaaaac tgaccctcct gcatttgcct
     5821 gagtctccaa ggaacggtga aaatctcgag ataatgtatc tgcaagttgt tgcctatcac
     5881 tgaaaatata gatttctagt ttcatcattc cctcctacta cggcaataat ccctaactac
     5941 tcaatctaca actcacaata ctcgccatct gttgccaggt ttttgcaagg atagcgccaa
     6001 tcctcttctg gattatcgaa aagttcatgc gcctctttgg gtccccaggt accggctggg
     6061 tagccataaa ttttgatgtc cggttgttct ctccaggcca ttaaaatagg atctacatag
     6121 cgccagcacg cctcaaccgc atcagtacga gggtaaaggg tcgcatcacc cagtatgcaa
     6181 tctagtagta aacgttcata ggcttctgga atatatgcat cgccaaggtc tgaataatgg
     6241 aagttcatat tgacgtcttt tatctggaaa ccggcgcctg gcattttcat tccgaattta
     6301 agcaagatgc cctcatcagg ttgaatgcga ataataagct cattacaact gacatcacaa
     6361 atattatttt gtttgaatag atgatggggg gtctttttaa aatggatgac cacttcagtt
     6421 actcgggtag gaaggcgttt tccagttcta atgtaaaatg gtacatctcc ccaacgccaa
     6481 ttgtcaatat ataatttgag tgctacaaag gtttcagtac gggaatctgg agcgactctt
     6541 ttttcattcc gatagcctgg aaccttttct ccgcgtatag ttgattctgt atattgaccg
     6601 cgcactgcat actttcttat ttcctcaggt ttgatgcgac gaagtgactg gaataccttt
     6661 actgtttcat tacgcactgc agtggcatcg aaaaacgaag gcggttccat tgcaattatt
     6721 cctacaatct gcaaaaggtg gttttgaacc atatccctaa gagcgccata gttatcgtag
     6781 tattcgcctc ggtcttccac accgatatgt tctgaagcag ttacttcaat atgatgaaca
     6841 taatttctgt tccacagtgg ttcaaaaatg ccgttagtga acctgaaggc taagatattc
     6901 tgtacagttt cctttcctaa gtaatgatca atgcgataga tttgcttctc atcaaaaatt
     6961 ttaagcagtg ttgcatttaa tttctttgcc gattctagat catgtccgaa tggtttttct
     7021 ataataatcc gcttccaaca gggaccattt ccctcctttt ctagattctg ttttccaagt
     7081 gattctgata tggttccaaa tagattaggt gaggtggata aataatatat gtaattgcca
     7141 cgagttttta attcagtgtc aatttgaata agccttgttt tcaattttgt ataatctgga
     7201 ctgttaactg cgtccaggga ctgataatag atatttttac aaaaggaatt tagcattttt
     7261 tcttctggtt gtttttcact atatagtttt atgccttcag tcattctatc tctaaatgct
     7321 gtatctggta gatccgaacg actaactcct aatacggcga agcgatcagg aagcaggttt
     7381 tgctcatata acgcaaaaag tgacgggatt agttttcgtt ttgttaaatc tcctgatgcg
     7441 ccaaaaatta caagtatgaa actatcaggt cgttccatat ttcttcgttg cctatgaaca
     7501 ctcttgaatt atttattctg catatttttt attctgtgag tttgtaattt aaataacaaa
     7561 accgcattag ttcacaggag cattttgacc atgattaaat tacaatttac ggcaaggttc
     7621 gaaatcgcct cagtgagata agaatacccc tgacatatga tacttaataa tgcaccttca
     7681 tcaacgaatg cttttaagaa gtgcattcac attctcagct actgtccctg atgaattata
     7741 cacacttgaa cctgctacaa gcactgttgc tccagcattt acaaccaaag gagctgtcgt
     7801 agtgtcaact cctccatcca ctgcgatggg tatataagag ctgcgttcgt caagtatccg
     7861 cctgagacgt cggattttat ccagttggct gtgaatgaat ttttgacctc caaagccagg
     7921 attaactgtc attatctgaa taaaatccgc tatatcaaga acctcttcta ttgcgttcaa
     7981 aggtgtacca gg
//
LOCUS       DATDRL010000075        14739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2643108, whole genome shotgun sequence.
ACCESSION   DATDRL010000075 DATDRL010000000
VERSION     DATDRL010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14739
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2643108"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..1625)
                     /locus_tag="VI935_02665"
     CDS             complement(279..1625)
                     /locus_tag="VI935_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24536.1"
                     /translation="MGNRRLITLIVTLAAICLFAVASGQVTGKKSMKQGPPLSGFDRA
                     ISQNAQRMIEEGRQIFRFDTFGDQAFWGDALKLHQEIVGAKLGGVGPGVSPKTALSVG
                     LKVDADALPLVLAKQIKAGKVNLDDPATTLALLKLNAVVGVTGFFDKQGNLRSVGIQC
                     ALCHSTVNDSFAPGIGQRLDGWPNRDLNVGAIIALAPDLMVVTGLLGVDDATVRKVLN
                     SWGPGKFDAELFMDGKAFRPDGKSAATLIPPAFGLAGINLSTWTGFGSVTYWNAFVAN
                     LEMHGKGTLFDPRLSNPEQYPVAAKAGFADVRNSPDLITPKLAALHFYQLAIPAPKPP
                     EGSFNKEAALRGKAVFESKTKCGSCHVPPLFVEPGWNMHKASEMGIDDFQASRSPDKR
                     YRTAPLKGLWTHKKGGFFHDGRFADLTAVVEHYNRLFKLNLTEQEKSDLVEYLKSL"
     gene            complement(1744..1941)
                     /locus_tag="VI935_02670"
     CDS             complement(1744..1941)
                     /locus_tag="VI935_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24537.1"
                     /translation="MFDNHFYNLMAQIVEEHKSLWRIKNMYKKDASGCKQCQTFWDKL
                     EKDKEDHIRELEEIIETHINK"
     gene            2164..2916
                     /locus_tag="VI935_02675"
     CDS             2164..2916
                     /locus_tag="VI935_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HLE24538.1"
                     /translation="MINHSGLLLIIFSLFLTSYRGAHAEPPFSDDIPILAKPYDESTF
                     IDIMGNEKMLDIFLKKETRSKSLADFEDFLRDTQNAYAGIWVFRALYVRQKDDQLIID
                     PRRWWKNISHFQNNGRRGPIAWNDGDGIMTNWVKHPAFGASAYLYYRALGYNRPTSAF
                     GAFLQSTLFEYTIEGIMRPPSLQDLIVTPGAGVPFGIILEETSNWLEDRDSGFLRALS
                     FVINPTKIFIKGSNNINLTPLMNQYVLIGFNW"
     gene            3044..3625
                     /locus_tag="VI935_02680"
     CDS             3044..3625
                     /locus_tag="VI935_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HLE24539.1"
                     /translation="MSKEEGASIAGVFWSLSPVVAITSSWQGRVNAQIAVTVVTASIV
                     HAYPRLIVGIWKGNYTHEFIINSRALAVHPLRRDQIEVVRNFGFYTGRDREKLKHIPY
                     DQGVTGSPILKDAHSYADCRVINAMDGGDMTAFLVNVVDGGILSGREWMTLDYFYNAA
                     PQEWIAEYSEKLSRSVALSLDRIHKIDYSTWIP"
     gene            3676..4143
                     /locus_tag="VI935_02685"
     CDS             3676..4143
                     /locus_tag="VI935_02685"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HLE24540.1"
                     /translation="MIEKESIEVINTHFVFPNDTNNYGTIFGGKLVELMDLTGALSAL
                     RFSGENVVTASIEALDFKIPINLADIVELRGKVIYSARTSMVVKIDVYREKKQGSAKD
                     FCCRGYFIFVAIDQNGRPKSIPKLKTVSEEDKRFWKIGETIKSRAHERRTASP"
     gene            4198..5547
                     /gene="thrC"
                     /locus_tag="VI935_02690"
     CDS             4198..5547
                     /gene="thrC"
                     /locus_tag="VI935_02690"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HLE24541.1"
                     /translation="MNNRNKYRVWFQCTNNTCNEIYPINEIIYRCKKCSNLLEVAHDL
                     KPLQEKSQGEWKQLFDNRYRRQVWPYGSSVWGKKEWVCPYVEDDNVVSMYEGATNLFW
                     AERFGRQVGVEDLWIKMCGNSHTGSFKDLGMTVLVSVVKQMIADGADIPAVACASTGD
                     TSAALSAYCAAAGIQAIVFLPRNKVSVAQLVQPLSNGALVLSLDTDFDGCMEMVQRIC
                     TEHNIYLANSMNSLRIEGQKTVSIELCQQFDWEVPDWVIIPGGNLGNVSALGKGFIIM
                     HELGLIDKLPRIVCAQAQRANPLYLSYLKGFNEFHPVKAEKTLASAIQIGNPVSVNKA
                     IKILKMFDGIVEQASEDELANATALVDRTGTFNCPHTGVATAAFLKLAERKVIKPTER
                     VVIISTAHGLKFVEFKIGYHEGRLEGIIPKHANTPLELPLDLAMVKKAIFENIKEAI"
     gene            complement(5675..6592)
                     /locus_tag="VI935_02695"
     CDS             complement(5675..6592)
                     /locus_tag="VI935_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLE24542.1"
                     /translation="MTAEAIVVASGISKSFGSIRAVDGISFQVMLRECYGFLGPNGAG
                     KTTAIRMVSCHIPLTSGILNVFGLDVSRRSREIKAMVGVAPQDNNLDPDLNVIENLVV
                     YSRYFDIPRREAERRSRELLEFMSIDERRKEQVTRLSGGMQRRLVVARALINEPKLLI
                     LDEPTTGLDPQARHLIWQRLRELKRKGVTMILTTHYMEEAEQLCDRVAVMDSGKILVE
                     GQPRELIERYAGRDVFEVRRAEDNRVLLRLEGLEFGFERAGDTIYIFSRDGDEIGRRL
                     LSIRELEVLHRRANLEDVFLRLTGRELRD"
     gene            complement(6720..7637)
                     /locus_tag="VI935_02700"
     CDS             complement(6720..7637)
                     /locus_tag="VI935_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24543.1"
                     /translation="MQAHKVSVLYFKTAVVFLVVLMGFCISANGVRAAGPMIPHSFFL
                     ENPYAYTGFRANVEVVDLDDPPGGVLIMNQYRVDINIFNNFFGLYAKFPFAGVIDFGP
                     DSDFNLTEKQDDYDFGNIGLGGKFALVNLDYLILTAGFEVILPTASDDLGAQAAQAYF
                     RDFVNFIDEAITLDPYLVLGVGSGMFALQGNLDFDIITNADKIDTIGSINTGGDDVEL
                     IIKYGGTASITPPLNLPFSTTVLVEVLAESTTSFDDNFTAVYVTPGLRFGGQIVSIGA
                     GVQIPVADNEVTDFANVDFLFDLIIRFGS"
     gene            complement(7847..8722)
                     /locus_tag="VI935_02705"
     CDS             complement(7847..8722)
                     /locus_tag="VI935_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24544.1"
                     /translation="MKILKSVLLPFALLVLISSTQGCGNAECVLTGFVNCGNDGGADK
                     SIINGTVVDVIGGPGVSGIKVIAERNGERVASDTTNSEGEFKLKVREGDITLLFEILD
                     AVTVGRVFTITEDSEVFLDVSLDLVQKQIEVEDWNVSQDPIRCGSNQSFIIAEEDLID
                     FTVNGNGKDCIRAKGDCLLIGITVQNIALNDCNEGILAEGSADVNLEALAVPTLTIDA
                     ESNGIHTKGNSSVTLIGGNIIVTSTQRDGIRAEDSSDVEIQPSGLCTIEGFDDAIDPD
                     PTATIDPDDCTLVQP"
     gene            9046..9672
                     /gene="thiE"
                     /locus_tag="VI935_02710"
     CDS             9046..9672
                     /gene="thiE"
                     /locus_tag="VI935_02710"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HLE24545.1"
                     /translation="MYILKGLYVITDQKLIPRDRFIEIIEKVIQGGAKIVQLREKDSP
                     DEEIIRLGKKLLKITKRHGIPLIINDSPKLAVEVGADGVHLGMDDPAIMEARRLLGSE
                     AIIGVSCYGEIERGLRAEREGADYLAFGTPFFTPTKPGRRPTPFEVLREAKRRITKIP
                     VFAIGGITKGNAKSVLETGVDGIAVITAVFSSPNPEEAARSLAEFFEK"
     gene            complement(10081..12600)
                     /gene="leuS"
                     /locus_tag="VI935_02715"
     CDS             complement(10081..12600)
                     /gene="leuS"
                     /locus_tag="VI935_02715"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HLE24546.1"
                     /translation="MKEAYNPQEIERKWQYIWGEKRVYEVTEDHNEEKYYVLEMFPYP
                     SGRIHMGHVRNYTIGDVIARYKRTLGFNVLHPIGWDAFGLPAENAAIERGVHPEKWIY
                     ENIAQMKAQLKRLGFSYDWNREITTSDPNYYKWEQMAFTRMFDRGLAYKRSSVVNWCP
                     SCETVLANEQVEDGRCWRCKSSVTPRELEGWFFKITDYTEQLLDGCERLKDGWPERVL
                     MMQKNWIGKSTGAEIEFPLEEAIGGVRSLRVFTTRPDTVFGVTFMCIAPENSISEKIV
                     KGKPNEKEVVQFIQRIKRLSNVERVAEGVKKVGAFTGAYCINPFSAERMPIYIANFVL
                     MEYGTGVIMCVPAHDQRDFEFAKEYCLPIKVVIQPIGALRELPLHPDDMKCAYEDVGT
                     MVNSGEFSGLPSEIGKIKVIEYAETRGVGKRQVNYRLRDWGISRQRYWGAPIPVIYCG
                     KCGILAVPDNELPVELPMDVEFTGKGGSPLANIDKFVNTKCYKCGGDARRETDTMDTF
                     VESSWYFLRYTSPDFEKGMLDGVRGKYWLPVDQYIGGIEHAILHLLYARFYTKVLRDI
                     GMCDLDEPFINLLTQGMVIKDGAKMSKSVGNIVDPDDMIAKYGADTVRIFMLFASPVE
                     RDLDWSDEGIEGAYRFLNRVWRLVYDFGIRDSGLKVGNAYMHSLQPQSAIRSPQSKDL
                     LIKVHKTIKKVTEDLERFQFNTGVAAIMELLNAVSRFQSNGNDEKAGLREAIESIVRL
                     LYPFAPHIAEELWEALYYKETLVDKPWIEWDREIVGGAAVIVVLQVNGKVRSQVFMDP
                     DTTEEEMKQAAFSDERVKSYISGKEIKKVIVVPKRLVNIVT"
     gene            13035..14522
                     /gene="dnaA"
                     /locus_tag="VI935_02720"
     CDS             13035..14522
                     /gene="dnaA"
                     /locus_tag="VI935_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004392913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HLE24547.1"
                     /translation="MAKKVTLGRNLKSLFKDENDKEPARGIGLLVSPIGNYSIATQKA
                     VFPQQNIWQEVIERIKKRANPQVFFWFDSLQPLSQTDELLTLKAKNHFERDWIYNHYL
                     DLISKTIEELHGRKLDIKIVAANPTSIEEEIEEVEKSREKNKETLFSGFLNPNYTFDR
                     FIVGASNQFAHAASIAVARKPGEAYNPLFIHGGVGLGKTHLINAIGNYILKNSHQLAR
                     VCSISAENFTNQVINSIKTSKMEEFRNRYRFGCDVLLIDDIQFIVGKESTQEEFFHTF
                     NTLYESKKQIVLTSDKTPKDMSYLEERLRSRFEWGLITDIQPPETETKVAILTKKAEA
                     EGIVLPNDVAIFLATNITSNIRELEGTLINISAYAKLLNTEINIDLAKEVLKNIIKEQ
                     TRCILNIESIQKEVANFFGVKIQDLKSEKKMKNIALPRQIAMYLTRKYTGASYPEIGE
                     KFGGKDHSTVIHAVRKIEAKIGEDSSLKDSVNAVVRKIEALIRSG"
BASE COUNT         4430 a         3221 c         2870 g         4218 t
ORIGIN      
        1 atcaatatct gaattttaag gagattagtt ctggtgaaac taaaaagatt aaaactcata
       61 ctctacccct tgagaatcta cttacaggtg acatttttac tttgcatcag gggtgacata
      121 ttcactttgt attgacagaa aaattctctc tctctagctg tctgtttctg attcatggat
      181 ttcctatact caattcgcca gtctttatga accgatttta tggacaagtt catatactct
      241 gattagtttg cttcttctac tctgttttgt cctgcaagct aaagtgactt caggtattcg
      301 accaaatcgc tcttttcttg ctctgtcaga ttcaacttaa aaagcctgtt atagtgctcg
      361 acaactgccg ttagatccgc aaagcggcca tcatggaaga agccgccttt cttgtgtgtc
      421 cagagaccct tcaacggcgc ggttctgtat cgtttatcag gcgaacgact cgcctggaaa
      481 tcgtcaattc ccatctcaga ggccttatgc atattccagc ctggctcgac gaagagtggc
      541 ggtacgtggc aggagccgca cttcgttttg ctttcgaaga ctgccttacc acgtaaggct
      601 gcctccttat tgaaggaacc ctccggtggc ttcggtgcag gaatggccaa ctgataaaaa
      661 tgtaaagcgg cgagtttggg ggtgattaaa tcaggactgt ttcgcacatc cgcaaacccg
      721 gctttggccg ccaccgggta ttgttccgga ttgctcaacc gaggatcgaa aagggttccc
      781 ttcccgtgca tttcgagatt tgcgacaaat gcattccagt aagtgaccga gccaaatcct
      841 gtccaggtgc taaggtttat accagcgagg ccgaaagcag gcgggattag ggtggctgct
      901 gatttgccgt cagggcgaaa agctttacca tccatgaata gctcggcgtc gaatttcccc
      961 ggcccccaac tgttgagtac ttttcggacg gttgcgtcat caacgcccaa aagaccggtc
     1021 actaccatca ggtcgggagc aagcgctatg attgcaccga cattcagatc gcggttgggc
     1081 cagccatcca gccgctggcc aatacccgga gcgaatgaat cattaactgt ggagtggcaa
     1141 agtgcacact gaattcccac agaccgcaag tttccttgtt tgtcaaagaa ccccgtaaca
     1201 ccgacaaccg cgttcagttt gaggagagcg agggtagtag ccggatcgtc cagattgact
     1261 ttgcctgctt taatttgctt agcaagaact agcggaagcg catcggcgtc cactttcaaa
     1321 cccacactca acgctgtttt cggacttacg cccggaccca cacctccgag cttggcaccc
     1381 acgatttcct gatgaagttt aagggcatca ccccagaagg cctggtcgcc aaaggtatcg
     1441 aagcgaaaga tttgtctgcc ttcctcgatc atccgctgcg cgttttgact aattgcacgg
     1501 tcgaaccccg agagcggcgg tccttgcttc atggacttct tgcccgtcac ttggccagag
     1561 gccacggcaa acaggcagat ggctgccagt gttactatga gcgtgatgag tcttcgattg
     1621 cccatatcct taaatcctcc tcatgttcct ttccaaaatt ggaaacaccc tcaaataaag
     1681 tgtcagtttt attttatctt ttgaaacttt tcaatttatt tgagacttat ctttttataa
     1741 ttcctatttg ttaatatgtg tttcgataat ttcctcaagc tctctgatat gatcctcttt
     1801 atctttttct aacttgtccc agaaggtttg acattgctta cagcctgaag catctttttt
     1861 atacatattt ttgatccgcc agaggctctt gtgttcttcg actatttgtg ccatgaggtt
     1921 gtagaaatgg ttgtcaaaca tcttttttac ctcctttccc ttttattctt ttttttctta
     1981 atcccatttt atacagaaaa ctttcctact actattttct tacaagtaag aagttcttgt
     2041 caaggaaatt ttggatgtat tccgatcaat cctattctct tacgcgttac ttgggattaa
     2101 aactactacc caaatcataa tcatggtgtt attatttgat ttagattttc gtggagaaga
     2161 tatttgatta atcattcagg tttattactt ataatattca gcttgttttt gacttcttat
     2221 cgcggtgcgc atgccgaacc accatttagc gatgacatac caatactagc taagccatat
     2281 gacgaatcta catttattga tataatggga aatgaaaaaa tgcttgatat atttcttaaa
     2341 aaagaaacca ggtctaaatc acttgccgat tttgaggatt ttctacgcga cactcaaaac
     2401 gcttatgctg ggatctgggt ttttcgagca ctttacgtaa ggcaaaaaga tgaccaactg
     2461 ataatagacc cgagaaggtg gtggaagaac atatcccatt ttcaaaataa tggacgtcgt
     2521 gggcctattg catggaatga cggcgatgga attatgacaa actgggtcaa acatcctgcc
     2581 tttggagctt ccgcttatct ttattacaga gcacttggat ataaccgccc tacttcagct
     2641 tttggtgctt ttcttcaaag tactctattt gaatatacaa ttgaaggcat aatgcgcccg
     2701 ccctcacttc aagaccttat tgtaacacct ggggctggag taccctttgg aatcattctt
     2761 gaggaaacct caaactggct tgaagataga gacagtggct ttttaagggc tttaagcttt
     2821 gtgataaacc ctactaagat atttatcaaa ggaagtaata atataaatct tacaccactt
     2881 atgaaccaat atgttttaat cggttttaat tggtaataga aatcattaat tcctgctatt
     2941 tgttaaacgc taaatataaa tgcgggctac ataagcccaa gaagaatgga ttaagctaat
     3001 actgtaagct ttaaaatata ttctaattta ggaagggatt ttgatgagta aagaagaagg
     3061 agcatccatt gcaggcgttt tttggtcact ttctccagta gtagcaatca caagctcgtg
     3121 gcagggtagg gttaatgctc agatcgcagt aactgtagtt acagcatcaa tcgttcacgc
     3181 ctacccaagg cttattgttg gaatctggaa gggaaactac acacatgaat ttataataaa
     3241 cagcagggcc ttagctgtcc atcctctcag aagagatcag atagaggttg taagaaactt
     3301 cggcttctac acaggtaggg atagagaaaa actcaaacac attccctatg accaaggagt
     3361 aacagggagc ccgattctta aagacgccca ttcctatgca gattgtaggg ttataaatgc
     3421 aatggatgga ggcgacatga ctgccttcct ggttaatgtt gtagatgggg ggatattaag
     3481 cggtagagaa tggatgaccc ttgattactt ctacaatgct gcacctcagg agtggattgc
     3541 ggaatatagc gaaaaactct ctcgctcagt tgctttatca cttgatagga ttcataagat
     3601 agattactca acctggattc catagttcac cattcagatt tgcattgaac cagttgacct
     3661 ataggaggat taaagatgat tgagaaagaa tcaattgaag taataaacac ccattttgtc
     3721 ttcccaaacg acacgaataa ctacgggacg atttttggtg gaaagcttgt tgagttgatg
     3781 gatcttaccg gggcattatc agcattacgt ttttcaggtg agaatgttgt cactgcatct
     3841 attgaagcgc tagatttcaa aattccgata aacctagcag atattgtaga attaaggggc
     3901 aaggttatct actcagctag aacctcgatg gttgtaaaga ttgacgttta tagagaaaaa
     3961 aagcagggta gcgcaaagga cttctgttgt agaggctatt ttatatttgt tgcaatagac
     4021 caaaacggga gaccaaagtc tataccgaag ctaaaaactg tttcagaaga ggataaaagg
     4081 ttctggaaaa ttggagaaac aataaaatcg agggcgcatg aaagacgaac ggcgagtcca
     4141 taaacaggtc ttgcacgttg acatacaaaa tccaaaggtg tattttgttc taaggaaatg
     4201 aataatagga ataaatatag ggtttggttt cagtgtacaa ataatacgtg taatgaaata
     4261 tacccaatta atgaaatcat ttatcgatgc aagaaatgca gcaacctttt agaagtagca
     4321 cacgacctaa aaccactcca agaaaaatca caaggcgaat ggaaacagct ttttgataac
     4381 cgttacagac gccaagtatg gccgtatggg agctctgtat ggggaaaaaa agaatgggtt
     4441 tgtccctacg tcgaagatga taacgttgta tcaatgtatg agggtgctac aaaccttttc
     4501 tgggcggaaa gatttggaag gcaggttgga gttgaggatc tctggataaa gatgtgtgga
     4561 aacagccata ccggctcttt taaagatttg ggtatgactg ttcttgtgtc agtcgtaaaa
     4621 caaatgatag cagatggggc ggatattcct gcagtagcat gcgcatcaac tggagatact
     4681 tctgcagcgc tttccgccta ctgcgcagca gcaggaattc aagctatcgt ttttcttccg
     4741 agaaacaagg tatccgttgc tcaacttgtt caaccgctct caaatggcgc ccttgttcta
     4801 tcactagaca cggattttga tggctgcatg gagatggttc aaagaatctg cactgagcac
     4861 aatatttatc ttgcaaactc catgaactcc ctacgcatcg aaggacagaa aactgtgagt
     4921 atcgaactct gccagcaatt tgattgggag gtgcctgatt gggtaatcat tcccggtggg
     4981 aatcttggaa acgtgtccgc tcttggaaag ggattcataa taatgcacga gcttggtctt
     5041 atcgacaaac tgcccagaat tgtgtgtgca caggcgcagc gcgcaaatcc actttatctc
     5101 agctacctca aaggatttaa tgaattccat ccagtaaaag ccgagaagac cctagcaagc
     5161 gctatacaga ttggaaaccc tgtaagcgtg aataaagcca taaagatact aaagatgttt
     5221 gatggaatag tcgaacaggc tagtgaagat gagcttgcaa acgctacggc gcttgtagat
     5281 agaactggga cattcaactg tccccacacc ggcgtagcga ctgcagcttt cttaaaatta
     5341 gctgaaagaa aggtaattaa acctactgag agggtcgtta taatctctac agcccatggt
     5401 ctaaaattcg ttgaatttaa aatcggatac cacgaaggca ggcttgaagg aatcatacct
     5461 aagcatgcta acaccccttt agaactccca ctggatttag ctatggtaaa aaaagcgata
     5521 tttgagaata taaaagaggc aatttgattt tccctatccc cctactaagc aaagaacaac
     5581 aattcagaag tctaaatcca agagtcagaa gcctaattga ctcttctgaa tacagaattc
     5641 tgccttccgt cttctgactc ttgtcttctc attcctaatc cctcaattct ctccccgtta
     5701 acctcaaaaa cacatcctcc aagtttgcgc ggcgatgaag aacctcaagc tctcttattg
     5761 aaagaagcct tcttcctatc tcatccccat cccttgaaaa gatgtaaatc gtatctccgg
     5821 ctctctcaaa accgaactca agcccctcaa gcctgaggag aaccctgtta tcctccgcgc
     5881 gtcttacctc aaacacatct cgtccggcat atctttctat gagttccctc ggttgtccct
     5941 ctacgagtat ctttccagaa tccattaccg caaccctatc gcatagttgt tcggcctctt
     6001 ccatataatg agtagtaagg atcatggtaa ccccctttct ttttaactca cgaagcctct
     6061 gccagataag gtgccttgcc tggggatcga gccctgttgt aggttcatca agaatcagaa
     6121 gcttaggctc gttgataagc gcccttgcta ctacaaggcg cctttgcatg ccgcctgaga
     6181 gccttgttac ttgttccttt cttctttcat ctatactcat gaattcaagt agttcccttg
     6241 atcttctctc cgcttccctc cttgggatat caaagtatcg agaataaaca acaagattct
     6301 caataacgtt taaatccggg tctaggttgt tgtcctgtgg ggctactcca accatagcct
     6361 taatttccct actacgccgg cttacatcta atccgaaaac attcagaatc ccggaagtaa
     6421 gaggaatgtg gcaggaaacc atacgaatag cagtggtttt ccctgctcca ttgggaccga
     6481 gaaaaccgta gcactctctt aacatcacct gaaatgatat cccatctact gcacggattg
     6541 aaccaaagct ttttgatata ccacttgcaa ctactattgc ctcagcagtc ataggtataa
     6601 ataggatacc ctaggtgagt aaaaaaaggt gactagaata taaagatatt attagcttat
     6661 taaataagct tatatcctaa tttgtgatta ataggagccc ttattaatgc ctacggaggt
     6721 cagctcccaa accttattat gaggtcaaaa agaaaatcta catttgcaaa atcagttact
     6781 tcgttgtcag caactggaat ttggactcct gcgcctatac ttactatctg cccaccaaac
     6841 cgaagccctg gggttacata aaccgccgta aagttgtcat caaacgatgt tgtagattct
     6901 gcaagcacct ctacaagaac tgtagttgaa aatgggagat taagaggagg tgttatgctt
     6961 gcagtgcccc catactttat tataagctca acgtcgtctc cacctgtgtt gattgacccg
     7021 attgtatcaa tcttgtcggc atttgtaatt atatcaaaat ctagattacc ttgtagggca
     7081 aacatcccag aaccaacccc taacaccaaa taggggtcta gggttatagc ctcatctata
     7141 aaattgacaa aatctctaaa ataagcctgt gctgcctgcg cacccaagtc atcgcttgct
     7201 gtgggcaata taacctcaaa accggctgtg agaattaagt aatcaaggtt tactaacgca
     7261 aatttgccac cgagtccgat atttccaaaa tcataatcat cttgtttctc tgttaagtta
     7321 aaatcagagt ctgggccaaa atcaattaca ccagcaaagg gaaactttgc atataagccg
     7381 aagaagttat taaatatgtt tatgtcaacc ctatattgat tcataattaa cacaccccca
     7441 ggtggatcat ctaaatccac cacctctaca tttgccctaa aacctgtata agcataggga
     7501 ttttcaagaa agaatgagtg gggaatcatt ggtcctgctg ccctaacacc atttgcagag
     7561 atacaaaagc ccattaaaac tactaagaat acaactgcgg ttttgaaata aagtacactt
     7621 actttatgtg cctgcatcct accatacctc cttattgagt ttataaaaga ttcaccttga
     7681 gaaatcgatt ttatcaaaac tctgtcacga tttcaagcct ttatcatttg tttatgaaaa
     7741 aaagcattta aaagtggaat ataggattta agtttctgat agataaagtt atgtcgcgaa
     7801 gctaagttgg cagaggtgaa acgcatttag ttgccaccta tatctactat ggttgtacaa
     7861 gtgtacaatc atctggatct attgtcgcag tgggatctgg atctattgca tcatcaaatc
     7921 cctcaattgt gcacagacct gatggttgaa tctcaacatc tgaagagtct tcagccctta
     7981 tgccatccct ctgagtagaa gttacaataa tattcccacc aataagcgtg acagacgaat
     8041 taccttttgt atgaatacca ttactttcag cgtcaattgt taatgttgga accgccagcg
     8101 cttcaagatt cacgtcggcg ctgccttcgg caagtatgcc ctcgttgcaa tcgttgagcg
     8161 caatattctg aacagtgata ccaatgagga ggcagtctcc tttagccctt atacaatcct
     8221 tcccgttacc attcactgta aaatctatta aatcttcttc ggctattata aaactttgat
     8281 tgctaccgca acgtatagga tcttgcgaaa cattccaatc ctcgacttct atttgttttt
     8341 gtactaggtc tagactcaca tctaaaaaaa cctcactgtc ctccgttata gtaaaaactc
     8401 taccaacagt aactgcatcg agtatttcga atagtagggt tatatcccct tcacgaacct
     8461 tgagtttaaa ttcaccctca gagttagtag tatcactggc taccctttca ccattccgtt
     8521 cagcaataac cttgattcca gaaaccccag gaccccctat aacatctaca actgttccat
     8581 taattatgct cttatcggca cccccatcat tgccacaatt aacgaagcct gttagtacgc
     8641 attcggcatt gccacaccct tgtgttgacg atattaatac cagcagggca aagggcaata
     8701 atacactttt taatatcttc atatatacca tcacatttag agagtagacg ataatatcac
     8761 gtagaataaa ctcctgtcaa gaaaacgcaa tcaataaccc tgcagcaagc tgcagggtat
     8821 ggtcttaaaa atataccgtt atagctgtca ttgcgaggag caaagcgacg aagcaatcta
     8881 aaatgagaga ttgcttcacg gagcctgcac tgaacgaaat gaacgtgttc gcaatgacaa
     8941 tacactattg tggaaaccct gtagcaagct acagggaatt ctcaagttaa atgtattaaa
     9001 tcaaaatttg aacaaatatt taatcaagat actataaaat ctgtaatgta cattctcaaa
     9061 ggactttatg ttataactga tcaaaaactc attccaagag ataggtttat agagataata
     9121 gaaaaagtca tccaaggggg agctaaaatc gtacagctta gagagaagga tagtccagac
     9181 gaagaaatca taaggcttgg gaaaaaactc ttaaagatca caaaaaggca tgggattcca
     9241 ttgataataa acgattcacc taagcttgca gtagaggttg gagcagacgg agttcatctg
     9301 ggaatggacg acccggccat catggaggct cgaaggcttc taggaagtga agcgataatc
     9361 ggggtttcgt gctacggaga gatagaaagg ggcttgcggg cagaaaggga aggagcagat
     9421 tatctggctt ttggaacccc gttttttacc cctacgaaac caggtagaag gcctacccct
     9481 tttgaggttc taagagaggc aaaaaggaga ataaccaaga ttccagtttt tgctattggt
     9541 ggaataacaa agggaaatgc aaaatcagtt ttagaaaccg gtgtagacgg gattgcagta
     9601 attacagcag tttttagctc gcctaaccct gaagaagcag cacggagtct tgctgaattt
     9661 tttgaaaaat aaacataaca tattatgttt tcgtacttca aatactaatc tagtaaaatt
     9721 taattgataa gctacctggt taggcgacga gccttggccc aaatctcagt acaggcttgt
     9781 tagttgcctg ctaacctctc tccagcaaga cctggtaagc tattgaacat agagcatgtt
     9841 taactttgag aggggcatac tacccacggt ggtcaaacca caaggctacg atggttctct
     9901 ctccaagcag gtcttttaga agcgtaacat ataaggttac gagtcccttc gacataactc
     9961 agggcaggct tgctcgtgac ctgttgtctg tctaccagca ataacatcaa gccacgatag
    10021 gtatagcgac aaaatgatgc tcttgctaaa atattaagaa acacctcaca accatttttc
    10081 ttatgtaaca atgttcacaa gcctttttgg aacgacgata acctttttga tttctttacc
    10141 agaaatatag cttttcaccc tttcatccga aaaagcagcc tgtttcatct cctcttctgt
    10201 tgtatcgggg tccatgaaaa cttgacttcg tacctttcca ttcacctgga ggactacgat
    10261 gaccgcggcg ccccctacaa tctctctatc ccattcaatc caaggcttat caactagagt
    10321 ttctttatag taaagggctt cccatagctc ttcagctata tgcggggcga agggataaag
    10381 aagacgaaca atactttcaa tcgcttccct gagtcctgct ttttcatcat tgccattaga
    10441 ttgaaaccta gatacagcgt ttagaagctc catgatagct gcgaccccag tattgaattg
    10501 aaacctttct aaatcttccg ttaccttttt aatcgtctta tgtaccttaa taagaagatc
    10561 ttttgattgt ggactgcgga ttgcggattg cggttgtagg gaatgcatat atgcattccc
    10621 tacctttaat cccgaatccc gaatcccgaa atcgtagaca agacgccaca cgcggtttag
    10681 gaatctataa gctccctcaa tgccctcatc gctccagtca aggtctctct caacaggaga
    10741 ggcaaatagc ataaagatac gtactgtatc tgccccgtat tttgctatca tatcgtcagg
    10801 gtcgacgatg tttccgacag attttgacat cttagcaccg tccttaatta ccatcccctg
    10861 agttagaaga tttatgaagg gttcatcaag atcgcacatc ccaatgtctc ttaaaacctt
    10921 tgtataaaat cttgcataaa gcagatgcaa aatagcatgt tctataccac ctatgtactg
    10981 gtctacaggg agccagtatt taccccttac accatctaac ataccctttt caaaatccgg
    11041 agaggtgtat ctaaggaagt accaggatga ctctacaaat gtatccatcg tatcggtctc
    11101 tcttcttgca tcaccaccac acttatagca ctttgtattt acaaacttgt ctatattagc
    11161 aagtggtgaa ccaccctttc ctgtgaactc tacatccatc ggaagctcta caggaagttc
    11221 gttatcaggt actgctaaga ttccacattt cccgcaataa attacaggga taggcgctcc
    11281 ccaatacctc tgacgagata tcccccagtc tctaagccgg tagttgactt gtcttttacc
    11341 aacccctcta gtctccgcat actcaatcac ctttattttg ccgatctcag aaggaagacc
    11401 tgaaaattca cctgaattta ccatggtgcc cacatcttca taagcgcatt tcatatcgtc
    11461 tggatgtagg ggcagctcgc gaagtgcacc tataggttga atcacgacct ttataggtaa
    11521 gcaatattcc tttgcaaact caaagtccct ttgatcatgt gccgggacac acataataac
    11581 acctgtgccg tactccataa gcacaaaatt ggctatgtaa atcggcatac gctctgcact
    11641 aaaggggttt atgcagtagg cacctgtaaa agcacccact ttttttacac cttcggccac
    11701 cctttcaacg ttgcttagtc tcttaatcct ttggatgaac tggactactt ctttttcatt
    11761 tggcttgcct ttgacgatct tctccgagat tgaattttcc ggtgctatgc acataaaagt
    11821 aacaccaaaa accgtatcag gacgggttgt aaaaaccctt aaacttctca cgccccctat
    11881 agcttcctca aggggaaatt caatctcagc gccagtgctt tttcctatcc agttcttctg
    11941 catcataaga accctttctg gccagccatc tttaagcctt tcgcacccat caaggagttg
    12001 ctctgtataa tctgtaattt taaaaaacca accttctaat tctctcggcg tcacactgga
    12061 tttacatctc caacatcgcc catcctccac ctgttcattt gccaaaaccg tttcacatga
    12121 agggcaccaa ttgacaactg aagacctttt ataagctagt ccgcgatcaa acatccgtgt
    12181 aaaagccatc tgctcccatt tataataatt agggtcagag gtagtaattt ccctattcca
    12241 gtcataacta aaaccaagcc tctttagttg cgctttcatt tgagcaatat tttcataaat
    12301 ccacttttca gggtgaaccc cacgctcaat agcagcgttt tctgcgggaa gtccaaaggc
    12361 atcccaacct attggatgga ggacattaaa ccccaatgtc cttttatacc gcgctataac
    12421 atctccaatt gtatagtttc ttacatgccc catatggatc ctgccagagg ggtatgggaa
    12481 catctcaagc acataatatt tttcttcgtt atggtcctca gttacttcat agactctttt
    12541 ctcaccccag atatactgcc attttctctc aatctcttgt ggattatacg cttctttcat
    12601 tatcacaaac tacgctcctt gctgtttagt cgatgttagt ggattgacca aatttaaata
    12661 ctacattact gcctttagag ggactcatta agcattttaa attaatgccc cctgagattc
    12721 aaatcgccaa acttactgat cataacagaa aacacagtag aataccgaaa acaaaggatg
    12781 atgtgctcta tataacccta cttcaatttt catttttaat gcgagagacc caattccaac
    12841 tctaattaat tgtaatcact aaattattta aaaatgcttg acaaccactt ttttttcctg
    12901 ttaggatttg attaatgcct caaatgtgtg actaatccta gttaaaaacg tttgaggtta
    12961 tccccttatt gcatagcaat ctatccacaa cctgttgata atttgtggaa atgttgttag
    13021 ctcatggagg agttttggca aagaaagtaa cgctaggtag aaaccttaag tcccttttta
    13081 aagatgaaaa tgataaagaa cctgcgagag gaatagggct tcttgtaagc cctattggca
    13141 attactctat agccacccaa aaggctgtgt ttcctcagca aaacatatgg caagaggtaa
    13201 ttgaaagaat aaaaaagagg gcaaacccac aggtgttttt ttggtttgac tctctacaac
    13261 ccttatcaca aactgatgaa ttattaaccc ttaaggctaa aaaccacttc gaaagggact
    13321 ggatatataa ccactatcta gaccttataa gtaaaacaat tgaagagctt catggtagga
    13381 agttggatat taagattgtt gctgcaaacc ctacttcaat agaggaagag atcgaagaag
    13441 tggagaaatc gagagaaaaa aataaggaga cactattttc tggttttctt aacccaaact
    13501 acaccttcga tcgatttatc gttggagcta gcaaccagtt cgcacatgcc gcttcaattg
    13561 cagtagcacg aaaacctggt gaagcttata acccgctttt tattcatggt ggtgttggac
    13621 tcggaaagac ccatttaatc aatgcgattg gcaattatat acttaaaaat tcacatcaac
    13681 tagcaagggt atgcagtatt tcagctgaaa actttactaa tcaagtgatt aatagcatca
    13741 aaactagcaa gatggaagag ttcaggaaca gatacagatt tggatgcgat gtactcctga
    13801 ttgatgacat acaatttatt gttggaaaag aaagcacaca ggaggagttt tttcatacat
    13861 tcaacacatt gtatgaatca aaaaaacaga tagtacttac tagtgacaag actccaaagg
    13921 atatgtctta ccttgaagag cgtcttagat caaggtttga atgggggctt atcactgata
    13981 tacaaccgcc tgaaactgag acgaaggtag cgattcttac gaaaaaagct gaagctgaag
    14041 gtattgtgct tcctaacgat gttgctatct tcctggctac taatattact tccaatatta
    14101 gagaacttga ggggacgctc ataaatatta gtgcctatgc gaaactacta aatacagaaa
    14161 tcaacatcga tctagctaaa gaggtcctga aaaacataat aaaagaacaa accagatgta
    14221 ttcttaatat agagtctatt cagaaagagg ttgccaactt ttttggtgtt aaaatacaag
    14281 atttaaagtc agaaaagaaa atgaagaata tcgctctacc aagacagata gctatgtact
    14341 taacaaggaa atacacagga gcctcttacc cagaaatcgg agaaaagttc ggtggtaaag
    14401 accactcaac tgtaatacac gctgtaagaa aaattgaagc taagattggt gaggattctt
    14461 cacttaaaga ctctgttaat gcagtggtta gaaaaattga agcattgatc aggagtggat
    14521 agaactgttc ataagcctgt ggtaaaagct gttaaagaaa ttgtggataa aaaagtttgt
    14581 ggataagtat ggatatgtct atgtgtttat caacagagtt atcaacccct taaggtaacg
    14641 ctatttaagg ctttcttata cctttccaca atttcacagc tactactact attactatat
    14701 atataaataa atattataat taggagtagg aaaaatgaa
//
LOCUS       DATDRL010000076         2120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_804739, whole genome shotgun sequence.
ACCESSION   DATDRL010000076 DATDRL010000000
VERSION     DATDRL010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804739"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VI935_02725"
     CDS             complement(<1..989)
                     /locus_tag="VI935_02725"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HLE24548.1"
                     /translation="MTEYNPFKALKEEMDNLVIGHEDVKTSLLLGIISREHIYIEGPP
                     GTAKTMLAEIISRAAQLNFFFYQLHRDTRLSELVGDLIISKEIHATGELIKQRIVKGG
                     ILTAEICLLDDISRAPGESLNVLLRILNERKFGEERIPLLTAIGTSNPTADEYYNEPL
                     DPANLDRFVLQIKARGLSYGKRWNEAKEVIKLYSNRPLEYDVPIRVNRKIFDEYYERL
                     STVIMPSEVQDVLIDFVTTMIEDYGLNESNSLITDRTFFVKSLKIIRSHALLHGRTNC
                     TLDDLYALKYMTAFRVPEEIFLKIDEILEDVISKKKLNRDPKSNIESPNSEFD"
     gene            complement(1138..1830)
                     /locus_tag="VI935_02730"
     CDS             complement(1138..1830)
                     /locus_tag="VI935_02730"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HLE24549.1"
                     /translation="MIVYYVKYKLKVIPIRDTIPSRRFPVVNTILIFVNSLVFFYELS
                     LGNSLDSFIKTYGLIPESLAGGTTFVDRVYPFFTSMFLHGGWFHLIGNMLYLFIFGDN
                     VEDRMGHFKYLIFYIFSGVAAAFSQILINTHSTIPMVGASGAISGVLGAYILYFPRSR
                     ILTLVPIFLFIQFVEIPALLFLLIWFVMQFFSGVYMLAVTNDTGGVAFWAHVGGFVAG
                     LILAKPFQKRGY"
BASE COUNT          676 a          397 c          393 g          654 t
ORIGIN      
        1 ctatcgaatt cagagttcgg tgattctata ttgctttttg ggtcccgatt gagttttttt
       61 ttagagatca cgtcctcaag gatttcatca atcttaagga atatctcttc gggtacacgg
      121 aaggcggtca tgtattttag ggcataaaga tcatcaagtg tacagttggt tcttccgtgg
      181 agaagagcgt gggaccttat gattttaagg gattttacaa agaaagttct atcagtgata
      241 agggaattcg attcattaag tccataatcc tctatcattg ttgtaacgaa gtcaatcaat
      301 acgtcctgaa cctcagaagg catgattaca gtgctgagcc tttcataata ttcatcaaag
      361 atctttctat ttacccttat ggggacatca tattcaagcg gtctatttga gtagagtttg
      421 attacctctt ttgcttcatt ccatctcttg ccataagaaa gcccacgagc cttgatctga
      481 agtacaaagc ggtcaagatt tgctggatct agaggttcat tatagtattc gtcagccgta
      541 ggattgctag tgccaattgc agttagaagg ggtatgcgtt cctcaccgaa ttttctttca
      601 ttcaaaatcc gaagaaggac gttaagagac tctccaggtg ctcgagagat gtcatcaaga
      661 agacaaattt ctgcagtaag tattccaccc ttcacaattc tctgcttaat aagttcgcca
      721 gtagcgtgta tttcctttga tattatgaga tcgccaacga gctctgagag cctagtgtcc
      781 ctatgaagct gatagaaaaa gaagttgagc tgtgcggctc ttgagattat ttcagcaagc
      841 atagtcttag cagtacctgg tggtccttca atatagatat gctccctgga tattatacca
      901 agtagaagag acgtttttac gtcctcatgc cctatgacga gattatccat ttcttctttg
      961 agcgctttaa atggattgta ttctgtcatt ttctaattta tcctccgact cgtcgattta
     1021 acacaataat aattctacat tattagatat ttttttgcaa tatacttacc ttagtgctct
     1081 gatgtttcat tcccaaatta atttctctta ggcagttcta aataaactcg cataatacta
     1141 ataacctctt ttctgaaagg gtttcgcaag aattaaaccc gcaacaaacc ctcctacatg
     1201 cgcccagaat gcaactccgc ccgtgtcatt agtaacagct agcatgtaga cgccgctgaa
     1261 aaactgcata acaaaccaga ttaggagaaa caataaagct gggatttcaa caaattgaat
     1321 aaatagaaat attggaacta gcgtaagaat cctagacctc ggaaaataca gtatataagc
     1381 acctaaaaca ccagatatag caccacttgc tccaaccatt ggaattgtgg agtgggtgtt
     1441 gataagaatc tgagaaaatg cagctgctac ccctgagaat atatagaaaa taaggtactt
     1501 aaaatggccc attctatcct ctacgttatc cccaaaaata aagagataaa gcatatttcc
     1561 tattaagtga aaccatcctc catgtaaaaa cattgaagta aaaaaagggt aaacacgatc
     1621 aacgaatgtc gtgcctcctg caagtgattc agggataaga ccataggttt ttataaaact
     1681 atctaagctg ttaccaagag agagctcata gaaaaataca aggctattaa caaaaataag
     1741 gatcgtatta acgacaggaa accgtctaga tggtattgta tcacggatag ggatcacttt
     1801 taatttatat tttacataat atacgatcac attcaaagaa aaatatgcat actaatagtg
     1861 cacaatgtat ttatagaact gcatgtgtag atttcaaaat attatgaaac tataagagtg
     1921 cataagcatt tcgatctcct caatttcaat gagtttttca tgagcgcctt attaattgta
     1981 acttactcat gagatgtgcc tttattttgg aaataatatt aaccagcttt acaaaaccgt
     2041 tgatttatat ttaggacttg ccatgtgtta ttttagggct taaaatgatt atccagttta
     2101 gacttggatg gaggagaaag
//
LOCUS       DATDRL010000077         5639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3388066, whole genome shotgun sequence.
ACCESSION   DATDRL010000077 DATDRL010000000
VERSION     DATDRL010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3388066"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            406..840
                     /locus_tag="VI935_02735"
     CDS             406..840
                     /locus_tag="VI935_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24550.1"
                     /translation="MSTSSTTSTPPPTPKISPSHVVTDFLEAIKRKDYGKTYEFISAG
                     YAANLDIEAYKANMKQFYEKFSWSLLNYQILGVQILGDHALVVAELEVQFKTLNSAKE
                     TQKKTSVQYGLIVVDDRWKISLSDCISNCESREAFTGQGLKQ"
     gene            864..1664
                     /gene="dapB"
                     /locus_tag="VI935_02740"
     CDS             864..1664
                     /gene="dapB"
                     /locus_tag="VI935_02740"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HLE24551.1"
                     /translation="MIKVAVTGVAGRMGGRIVSLLREEDGIKIVGATEKEGHSSIGKD
                     IGNLAGGAEIGVHISDKIEVAASNADAIIDFTTPVATLKNALYASKKGKGMVVGTTGF
                     LEEEKKRFEKLAEAFPCIMSPNMSIGVNVMFEVSKLIAWHLGKDFDVEIIEAHHKHKA
                     DSPSGTALRLGEAIAEALGRDFNKVARFERHGRIGERGKEEIGIQAIRGGDIIGEHTV
                     IFCGTGERIEFTHRALSRDNFAMGAIRALKWVVGKPPGIYTMKDVLGI"
     gene            1795..2910
                     /gene="tgt"
                     /locus_tag="VI935_02745"
     CDS             1795..2910
                     /gene="tgt"
                     /locus_tag="VI935_02745"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HLE24552.1"
                     /translation="MFKFEILNKDGSTNARRGMIYTAHGLVETPAFMPVATQGSVKAM
                     TPLEIERFGFEIIVVNAYHLYLRPGFRTVEKLGGLHGFTSWNRSILTDSGGYQVLSLS
                     RVRKIKEEGILFRSHLDGSEHFLTPQKSIEIQKALGADIMMCLDECPPHPSTYEYLYN
                     SLELTSRWARICKETKGDSESALFGIVQGGVFSELRKKSVLELLEIGFDGYAIGGLGV
                     GESKEETCEMVVLSLSFLPEDKPMYLMGLGLPEDIVEAVSMGVDIFDCVLPTRNARNG
                     TLFTNHGKMVIKNAQYAEDETPVDEDCECYTCRNFSRAYLRHLYQVGEILASRLLTFH
                     NLHFYGRLMKEIRESLSRGQFSSFRTRFQSIGGFDKS"
     gene            2907..3299
                     /gene="yajC"
                     /locus_tag="VI935_02750"
     CDS             2907..3299
                     /gene="yajC"
                     /locus_tag="VI935_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HLE24553.1"
                     /translation="MRKIILVLMVSLWLAGCPIPPGGGEGMGGSPITAILPFVLIFVL
                     FYFLILRPQQKQQKSKQEMLKNLKRGDMVLTTGGVYGKILNIVEDVITLEIAKGVSIR
                     VARGGISGLGNPGKDEEEKKGKEEKSEK"
     gene            3296..4903
                     /gene="secD"
                     /locus_tag="VI935_02755"
     CDS             3296..4903
                     /gene="secD"
                     /locus_tag="VI935_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HLE24554.1"
                     /translation="MRRAYRGWIIVILIALLLSVIFLTPTFLGDMLPGWWGKVFPQKG
                     LRLGLDLRGGIYLLLGVRAEKAVEQELSNMKELMNEELANEKVLPKSFQNSDKTLTIS
                     FFSKSDLEKARKLESNYKEIADSRENDLSLSFTLKDQYIKELQRRAIDQVKETIDNRI
                     DEFGVVEPSIQRAGADRILIQVPGATEGDRERIINIIRRAAVLEFKIVKDSGPTQETL
                     LAKYNVTTPEELLSQALRLHPNDPSDIKSENEKFFITESDAQVTGQYLSDARLIFDEY
                     GRPAVGFSFRGEGASKFGKLTGDNIGQRLAIVLDGTIKSAPVIQERISSQGRITGTFT
                     LDEAKDLALVLRSGALPVPVDVEQEQTVGPSLGRDSIEKGKLSMIVGGVAVVLFMIIF
                     YKLQGVIANIALALNMLFIMGFLSSFGVTLTLPGIAGLVLTIGMAVDGNIITFERIKE
                     ELRVGKSPLAAIDAGYERSLWTVLDSNITTLLTALILFWFGTGPIKGFAVTLSVGIIF
                     TIFSNVIVARVIADLIYGGKKVEALSI"
     gene            4995..>5639
                     /gene="secF"
                     /locus_tag="VI935_02760"
     CDS             4995..>5639
                     /gene="secF"
                     /locus_tag="VI935_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HLE24555.1"
                     /translation="MDLIKPGTHIDFVGKMKKAVFISWALILIGIISLVFHKGPNWGV
                     EFVGGTEIHVKFAKDIKLDDIRSILSSAGYSAESVQQLGLPKDNEYLVRFSPKVVAFE
                     KIQEFQGQIAGIFDNVVGQGGTSILRIDYIGPKVGTELIEKAIISIILGWVGILLYLM
                     IRFKFEFALGAVIAIIHDILITLGAISITDKEFTLTIVAALLTVIGYSVNDTIVI"
BASE COUNT         1692 a          948 c         1369 g         1630 t
ORIGIN      
        1 aataataaga aagaatgccc agtcgcttga tagaaatatt tgaaaggcta aagttgtagt
       61 ggagaagaga ttagatgagt ccatacacat aaagtacaaa gatcaatatc tgaattttaa
      121 ggagattagt tccggtgaaa ctaaaaagat taaaactcat actctacccc ttgagaatct
      181 acttacaggt gacattttta ctttgcatca ggggtgacat attcactttg tattgacagc
      241 tatggtatag agtcatggca tagagtcatt tataaaggag ttttcttgaa attcttctta
      301 ttaacactta tcctatttcc cagtttgaga atattaagaa gtaagaaaca agtgagattg
      361 atttctttgt taatatgtct aatggcattt tcagcatgta ttggcgtgtc tacatcatct
      421 acaacctcga ccccacctcc tactcctaaa atttctccat ctcatgttgt tactgatttt
      481 cttgaggcta taaaaagaaa agattatgga aaaacctatg agtttatttc agctgggtat
      541 gcagctaatc ttgatataga agcctacaag gcaaatatga agcaatttta tgaaaaattc
      601 agttggagct tgctcaatta tcagatttta ggggttcaaa tactgggtga ccatgctctt
      661 gtagttgctg agctcgaagt gcaattcaag acattaaatt ccgcgaaaga aactcaaaaa
      721 aagacaagtg ttcaatatgg gcttattgta gtagatgata gatggaagat ttctctttct
      781 gattgtatct cgaactgtga atcaagagag gctttcactg gacaaggctt gaaacaataa
      841 ccctatctga gctgggaact tcaatgatta aagtagctgt tacaggagtt gcgggtagaa
      901 tgggcggaag gatagtttcg cttcttcgag aagaagatgg tatcaagatc gtaggagcaa
      961 ctgagaagga aggtcattct tctataggaa aagatattgg aaatcttgcg ggaggagcgg
     1021 aaataggggt tcacatatca gataaaattg aagtagccgc tagcaatgca gacgctataa
     1081 ttgattttac gacacccgtc gcaaccctca aaaatgccct ttacgcatca aaaaagggaa
     1141 agggtatggt agtagggacc accggtttct tagaagaaga gaagaaaaga tttgagaaat
     1201 tagcagaggc atttccctgt attatgtcgc caaatatgag tatcggagtg aatgtgatgt
     1261 ttgaggtgtc aaaattgatt gcttggcatc ttggaaagga ttttgatgtc gaaatcatag
     1321 aagcacatca taaacataaa gctgattcac caagtgggac ggctctcaga ttaggtgaag
     1381 cgatagcaga ggctcttgga agggatttta ataaggtggc gaggtttgag agacatggta
     1441 gaattggaga gaggggaaaa gaagagattg gtatacaggc catccgtggt ggtgacatca
     1501 taggagagca tactgtcata ttttgtggca caggtgaaag gattgaattt acccataggg
     1561 ctttaagtcg tgataatttc gcaatgggtg ctatacgtgc acttaaatgg gttgttggta
     1621 aaccccctgg aatctatacg atgaaggacg ttcttggaat ttagtgaaat agttaggaga
     1681 aaataatatt agccacgtat gaacactaat taacacgagt aagtgcctta aaattataaa
     1741 aatatttatt cgtgaagact tgtgtgtgca ttcgtggctt tcgtttatca caatatgttc
     1801 aaatttgaga ttctaaataa ggatggatct acaaatgcaa ggcgcggcat gatttatacc
     1861 gctcatggtc tagttgaaac gcctgcgttt atgcccgttg caactcaggg ctcggtaaag
     1921 gccatgacgc ctttagagat tgaacgcttt ggattcgaga ttatagttgt caatgcttat
     1981 catctgtatt tgagacctgg tttcaggact gtagagaaac ttggaggact tcacgggttt
     2041 acatcctgga atcgttcgat tcttacagat agcggtggct atcaggtgct tagcctttca
     2101 agggtaagaa agattaaaga ggagggaatt ctttttcgtt ctcacttaga tggtagtgaa
     2161 cactttttaa ctcctcaaaa gtcaattgaa atacaaaagg ccttgggggc tgacattatg
     2221 atgtgcctag atgagtgtcc ccctcatcca tcaacttatg agtacttgta taattcactt
     2281 gagttaactt cacgttgggc aaggatttgt aaagagacaa agggtgattc agaaagcgcc
     2341 ctttttggta ttgttcaggg tggtgtgttt agtgaactta gaaaaaagtc agtgctcgag
     2401 ctacttgaaa ttggcttcga cggttatgct attggtggcc taggtgtcgg cgaatctaag
     2461 gaagaaacat gcgagatggt agtgctttct ctttcctttc ttcccgagga taaacccatg
     2521 tatcttatgg ggcttggatt gcctgaagac atagtagaag cagtttcaat gggggttgac
     2581 atctttgatt gtgtgcttcc tacaagaaat gcccgaaacg gtactctctt tacaaatcat
     2641 ggtaaaatgg ttataaagaa cgcccagtat gcggaggatg aaaccccggt tgacgaggat
     2701 tgcgaatgct acacgtgcag aaatttctca agagcctatc taaggcatct ttaccaggtg
     2761 ggtgagatac ttgcctctcg gcttcttact tttcataatc tccattttta tggaaggctg
     2821 atgaaagaga taagagagtc tttatcaagg gggcaattct ctagttttag aacacgattc
     2881 caatcaatag gaggctttga taagagttga gaaaaataat tttggtacta atggttagct
     2941 tatggctcgc agggtgcccc ataccgccag gaggaggaga gggtatgggt ggttctccaa
     3001 tcactgctat cttacccttt gttctgatat ttgtcttgtt ttatttcttg atactaagac
     3061 ctcagcagaa gcagcagaag tcgaagcaag agatgctaaa aaaccttaaa agaggggata
     3121 tggtgcttac gacaggaggc gtttacggga agattttaaa tatcgttgag gatgttatta
     3181 cccttgaaat agcgaagggc gtaagtatcc gtgtggctag gggaggcata tctggtttgg
     3241 gcaacccagg taaggacgag gaagagaaaa agggaaagga ggaaaaatct gagaaatgag
     3301 aagggcttat aggggttgga ttattgtaat actgattgca ctgctactct cagtaatttt
     3361 tttaaccccc acattccttg gagatatgct cccagggtgg tggggtaagg tatttccaca
     3421 gaaggggctc aggttaggat tagatttgag gggagggatt tatcttctcc taggggtaag
     3481 ggctgaaaaa gctgtagagc aagagctttc taacatgaag gagttgatga atgaggagtt
     3541 agcaaacgag aaggttctgc caaagagctt tcaaaacagc gataaaacgc ttactataag
     3601 tttcttctct aagagcgatc ttgaaaaggc aagaaagtta gagtctaact acaaggagat
     3661 tgcagatagt agagaaaatg atttgtctct tagcttcaca cttaaggatc aatacataaa
     3721 agagctacag agaagggcta tagatcaggt taaagaaact atagataacc gtatagatga
     3781 gtttggcgtg gttgaacctt ctatccagag agccggtgca gacaggatat taattcaggt
     3841 tcctggggca accgaaggtg atagagaaag gattataaat ataatcaggc gagccgcggt
     3901 tcttgagttt aaaatcgtaa aggactctgg ccctacccaa gaaacccttt tagccaaata
     3961 taatgttaca acacccgaag aacttctctc gcaggcactt agacttcatc caaatgatcc
     4021 aagcgatatt aaaagtgaga atgaaaaatt ctttattaca gaaagcgatg cccaggtcac
     4081 gggacaatat ctttcagatg caagactcat attcgatgag tatggtagac cagcagtcgg
     4141 ttttagcttc aggggcgaag gagcaagcaa gtttggaaaa cttacaggag acaacatcgg
     4201 acagagactt gcgattgtgc ttgacggcac cataaaatct gcgccggtta tccaggagag
     4261 aatctcttct cagggaagaa ttacagggac ttttacactc gatgaggcta aggatttagc
     4321 ccttgtactt cgttctggtg ccctaccggt acctgtggat gttgagcagg agcaaacggt
     4381 aggaccctca cttggtaggg attcaatcga gaaagggaag ctctctatga tcgtaggcgg
     4441 ggttgccgtt gtgcttttta tgattatttt ctataagctt cagggtgtga ttgcaaacat
     4501 tgcactcgct cttaacatgc tttttatcat ggggtttctc tcatcatttg gggtaactct
     4561 tacacttcct ggaattgcag gacttgttct aacaataggg atggcagtgg atggaaacat
     4621 aatcacattt gaaagaatta aagaagaact tcgagtgggg aaatctcctc ttgccgctat
     4681 tgatgcagga tatgagaggt ctctttggac ggtgctcgat tctaatataa ctacattact
     4741 tactgctctt atactattct ggtttggaac gggtcccata aaaggctttg ccgtaacact
     4801 atctgttggg ataattttca cgatatttag caatgtgatt gtggcacgtg ttatagctga
     4861 tttgatctat ggagggaaaa aggtcgaggc tttaagcatt taggaattgg gaatatcgtc
     4921 aggcgtcagg tggcctaccc ctagcacctt gcgccctcac gactaacgcc tacgaattag
     4981 gaggttttaa taagttggat ttaatcaagc ctggtactca cattgacttt gtaggaaaga
     5041 tgaaaaaggc cgtttttatc tcttgggcat taatcctgat tggtattatc tctcttgtct
     5101 tccataaggg tccgaactgg ggtgtagagt ttgttggcgg gacagagatt cacgtaaaat
     5161 ttgcaaaaga tataaaattg gatgatataa gaagtatttt gagttctgcc ggatacagtg
     5221 cagaatcagt tcaacagctc ggtttaccta aggataatga gtacctcgta aggttttccc
     5281 ctaaggtggt tgcttttgaa aaaatccagg aattccaagg acaaatagcg gggatttttg
     5341 ataatgttgt tgggcaaggg ggaacgagta ttctcaggat tgattacatc ggtcccaaag
     5401 tcgggacgga actcatagag aaagcaataa tatcaattat actgggctgg gtcggtatac
     5461 ttttatacct gatgattagg tttaaatttg aatttgccct tggtgctgtt atagcaatca
     5521 ttcatgacat tctcataaca cttggtgcta tctctattac tgataaagag ttcacgctta
     5581 ccatagttgc tgcgcttctt acggtaattg gatattccgt aaatgatacg atcgtgatt
//
LOCUS       DATDRL010000078         2583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_139836, whole genome shotgun sequence.
ACCESSION   DATDRL010000078 DATDRL010000000
VERSION     DATDRL010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139836"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VI935_02765"
     CDS             <1..548
                     /locus_tag="VI935_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HLE24556.1"
                     /translation="LQERLAKLAGGVGVIRVGAATEAEMKNKKALVEDALHATRAAVE
                     EGIVPGGGVTYIRAIKMLDALEIGDEEEKAGVNIVKRALEDPVRMIADNAGWDGSIVV
                     QKIKEGKGSYGFDAARLEYCDLVEAGILDPTKVARVALQNASSVAGLLLTTEALVAEK
                     PKEEKKEGGMHGHPHMGMDEY"
     gene            complement(577..1467)
                     /gene="speB"
                     /locus_tag="VI935_02770"
     CDS             complement(577..1467)
                     /gene="speB"
                     /locus_tag="VI935_02770"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HLE24557.1"
                     /translation="MIQGNFIPINFLGLDENSSRFETSKVVILPVPFERTVSFGRGTS
                     RGPSSIIYSSRCLELYDEELGCEPYTCGVHTLAELECGSVDPAHMVSAVSEVCSQLLG
                     LNKFIITLGGEHSITLGAIKGHKTRFKDFSVLNLDAHCDLRDSYEGTPFNHACVMRRV
                     LEEGYNVVEVGIRSYSKEEAEFIKGNKNLKVIHAREIFKSKNQEWMDEVISSLLPQVY
                     ISVDLDVFDASFVPSTGTPEPGGLGWYDVTELIKTVFKKRKVIGFDIMELAPFSGLIG
                     PDVLAAKLAYKAIGFKFSKK"
     gene            complement(1575..2222)
                     /gene="cofC"
                     /locus_tag="VI935_02775"
     CDS             complement(1575..2222)
                     /gene="cofC"
                     /locus_tag="VI935_02775"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HLE24558.1"
                     /translation="MRFALIPVKDLSKAKERLYPILSQSERTDLAYAMLEDVLIALRG
                     SRLLDRILVVTLDENAIKLAVSLDLEVIKETKQNGESESVDLASTICKEMGAESVLVI
                     PGDAPLITPEDVDFILEKEKPYHSVILVPARDELGTNAILRKPPDVIPSRFGYDSFNK
                     HIEEARKRNIPYEIHKLPRVALDLDEPEDLSLFASQKSETTTYRELIRIGFIRGL"
     gene            2453..>2583
                     /locus_tag="VI935_02780"
     CDS             2453..>2583
                     /locus_tag="VI935_02780"
                     /inference="COORDINATES: protein motif:HMM:NF036998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HLE24559.1"
                     /translation="MALLTDLYEFTMADSYLRGKRNEEATFDLFIRHLPENRSFLVSA
                     "
BASE COUNT          761 a          505 c          568 g          749 t
ORIGIN      
        1 agcttcagga gaggcttgca aaattggcag gtggggtagg ggtgataagg gtaggagcgg
       61 caacagaagc tgagatgaag aataagaagg cattggtaga ggatgcctta catgcgacaa
      121 gggctgcggt ggaagagggt atagtgccag ggggaggagt aacatatata agggctataa
      181 agatgttaga tgcacttgag ataggagatg aagaagaaaa agcaggtgtt aacattgtta
      241 aaagagcgct tgaagaccca gttagaatga tagctgataa cgcaggctgg gatggatcaa
      301 tagtggtaca gaagataaag gaggggaagg ggtcatatgg atttgatgca gcgaggttag
      361 agtattgtga tttagtagag gcggggatac tggacccaac gaaggtagca agagttgcgc
      421 tacagaatgc gtcaagcgtg gcgggacttc tacttacaac agaggccctt gttgctgaaa
      481 agcccaagga agagaagaaa gaaggaggga tgcacggtca tccccatatg ggtatggatg
      541 aatattaatt cagtgggggc acgtctcaac gtgcccctat tttttactga acttaaaacc
      601 tatagcttta tatgcgagct ttgctgcaag aacatcggga ccgattagcc ctgagaatgg
      661 cgcaagctcc ataatatcaa atccaataac ctttcttttt ttaaacaccg ttttaatcag
      721 ctcagtaacg tcataccaac ctaacccacc gggctctggt gtgcctgttg aaggaacaaa
      781 ggaggcatca aatacatcaa gatcaacgct tatataaact tgtggaagaa ggctagatat
      841 aacctcatcc atccattctt gatttttgct tttaaagatt tctcttgcgt gaatgacttt
      901 tagattttta ttccctttga taaactcggc ttcctctttt gaataacttc ttataccaac
      961 ctcaactaca ttatatccct cttctaaaac ccttctcata acacatgcgt ggttaaacgg
     1021 agttccctca tatgaatctc tcaaatcaca gtgtgcatca agatttaaaa ctgagaaatc
     1081 tttgaaacgt gtcttgtgtc cttttatggc tcccagagta atcgaatgct caccgcctag
     1141 ggtaattatg aacttattta atccaagtag ttgagaacag acctcactga ctgcgcttac
     1201 catatgagcg gggtcaacag aaccgcattc aagctctgca agcgtatgaa ctccacaagt
     1261 ataaggttca catccgagtt cctcatcgta aagctccagg caccttgatg agtaaattat
     1321 agaggagggt ccccttgatg tccctctacc aaatgatacc gttctctcaa aaggtacagg
     1381 aagtataacg acctttgatg tctcaaatct tgagctgttt tcatcaagac caaggaaatt
     1441 aataggaata aaatttccct gaatcatgga agaaagagtt taatggttag gtgctcgttg
     1501 tcaatttact tgtgaatcgt tgctcgtgca tcgagtggtt cgactgggct catgaggaag
     1561 tcacgaatca cgactcacaa accacgaata aacccaattc taatcaattc tctgtaggta
     1621 gttgtttcac tcttttgtga tgcaaaaagt gatagatcct caggttcgtc taagtcgagc
     1681 gccactctgg gaagtttatg tatttcgtat gggatatttc tctttcgtgc ttcttcaata
     1741 tgtttgttaa agctgtcgta tccaaatcga gatggaatca cgtcaggtgg ttttctaagg
     1801 attgcgtttg ttccgagttc atctctagca gggactagaa tgactgaatg gtagggtttc
     1861 tccttctcta gaataaaatc cacatcctca ggtgtgataa gtggagcgtc tccagggata
     1921 actagaactg attccgcacc catctcctta caaattgtag aagccaagtc tacagattcg
     1981 ctttctccat tttgtttagt ttctttaatt acttctaaat ctaagctcac agcgagttta
     2041 atagcatttt catccaaggt cacaactaag atgcggtcta atagtcttga gcccctcagt
     2101 gctataagca cgtcctctaa catagcatag gcgagatccg ttctttcact ttgagataga
     2161 attggataaa gtctctcctt tgcttttgat aggtccttta caggtataag agcgaatctc
     2221 attactaaaa aatttacagg ggtttttttg tgtttactat aaataatttc aacaaggtga
     2281 tatgttccta caaattacac tattttcttg acgtgcttag cggctacttc tacaatcgct
     2341 ctactatttt gtttgatata aaaatccacg taagctaaat ttgtaagaat ctctcccttt
     2401 aatcgcgaca atgaataaaa agcttcattt tggatgggtt gacgaagaca acatggcgct
     2461 tcttacagac ctctacgagt ttactatggc tgatagttat ctaagaggca aaagaaatga
     2521 ggaggcgacg tttgatcttt ttatacgtca tcttccagag aacaggtcat tcctagtaag
     2581 cgc
//
LOCUS       DATDRL010000079         8609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_320251, whole genome shotgun sequence.
ACCESSION   DATDRL010000079 DATDRL010000000
VERSION     DATDRL010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320251"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..593
                     /locus_tag="VI935_02785"
     CDS             186..593
                     /locus_tag="VI935_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24560.1"
                     /translation="MFYLGQFSFHEEREKNTYYGFFNCIVEAENLEKAVDLFNKLLKS
                     TRKSDDLFDGAISIFLDEIFEIERIPKDGVLTTYMSIRGDMPAMLRCSIFNEAPGIQC
                     YGWTEDQDLEEEKEKASKDAVAQLPFLKFAEKA"
     gene            708..899
                     /locus_tag="VI935_02790"
     CDS             708..899
                     /locus_tag="VI935_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24561.1"
                     /translation="MLKEAGNFSFHALSYAEHLFDIRRGKGLLIERLPVEARGHGGFR
                     PSAHERAGNGELEAQAHVC"
     gene            1149..1570
                     /locus_tag="VI935_02795"
                     /pseudo
     CDS             1149..1570
                     /locus_tag="VI935_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611592.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1988..3253
                     /locus_tag="VI935_02800"
     CDS             1988..3253
                     /locus_tag="VI935_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HLE24562.1"
                     /translation="MKSSKNKTAFKLGNMSRSISLRMQYLGSKARIVDWILHEIEKRL
                     PKIDSFVDAFAGTGVVGAAAVKCGYRLYANDIQPYSYCILQAMFAQPTKGLDKAIADL
                     DAIANVSIASIFGSNIAAYAEEEDCFFSEYKNGKLNWKKYITFCEQTPIITPTKDFIL
                     SKTNNKAYDLFVTYYSNTYFGVRQCVQLDLLRKIASQYPENVTYHIIAATLAAMTYAV
                     SSTTHLAQYLKPSSASTAQNLIKKRSINIIADVATRLAGLSKTKPTTTPDCIYNSDFR
                     VALNSIKPGSKHVVYVDPPYFKEHYSRYYHVLDTFCLYDYPMLTYNPRTDAVTSGRYR
                     EGRIVSEFGLKSSVKKAFSDLFKLGVSHRTNIVISYAETSLVGKNELISIANANGYNA
                     DVKEVTLMHSGQGQPRHNLVREYLFICTL"
     gene            3264..4856
                     /locus_tag="VI935_02805"
     CDS             3264..4856
                     /locus_tag="VI935_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HLE24563.1"
                     /translation="MKDVLSLIKRLENISPEHDTPVGLIHPYWARKPLNVIQQLVREF
                     SENGDIVADPFVGSGTTVFAALSEGRKAIASDLNPLAIFVTKSIVELLESPEDKLKAA
                     HHFVEDFSDEVLPMFQYCEDYYVERERFVVKGEFEDGGYELISTEVVLKKKTGKSLKG
                     RRIEKPTSNWTDKKITASLSNSPINFGSLKLRPNSRIAIPNGATLAHYYEYKNQIAIN
                     LALQLIASKKYGRKNENTLKLLLSASLPLLRLSDKKASSQWPYWRPKEHLTSRNPIPV
                     LMKKLQSIEKAIKWLKTNVEGKDKWKLSDRVQLYNTSIQSLVPKYLEPERVDLVLTDP
                     PYSDQAPYLEYSALWIELIGLKLPLNAYKHEIVKTDAPSRVADTDDYIPRLREGLKAC
                     AHLLKPEGTLIWFYQDHNIEHWAALDEEALVNGLRILDVVPLAKQRRSMKTVTSPGAT
                     LDGDLILIFKKTNVVAKRPHTTEKVRRIILNELAPHSASSSYFEKYAAIVKSGLKFHL
                     FDLLSQSDKDIRKILSSMDNWR"
     gene            4858..7218
                     /locus_tag="VI935_02810"
     CDS             4858..7218
                     /locus_tag="VI935_02810"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HLE24564.1"
                     /translation="MYWLLSEEEQKKVRGIVNTLIVDLSVIVGNPQTDQLFPFVGRKD
                     RRKAESIVKHLSPKNSVVADPFSGSGIHIYAILSLGRVAIANEWEPYANRLSSAPWCL
                     PQKSALEFAYNDLIEKIQPELDKLYWTKCACGADHVFKSLFYDREPLKYTAVTNHERL
                     GPNGENVEYRGKYKCPNCGSREKFFDKNDAKHLSKLSTLPTDPIFDTTLIENSRINLS
                     KAFTVYGALFPHRSKLALSIIWKNIQQLQCQSIVKSFLEDVFLSILPQAKYKDYRSKS
                     QDLHCPPEKLRENNLLYVYQENYHKRWEGLKTYKFATADKAKTAINPIKMSDYRDFFN
                     SLGDESIDLVFTDPPWIDGTAYFERAQLYHPWMGYDLKKDKLRLEKEMVVSDAPTRKE
                     VHGIERWWDDLEVFFQSSYRVLKATQFIALFFRPIPADQWLSNLNRIKLVARRAGFEP
                     LLSIDVKSSDPSMRIQQSASYVFSKDVVMLFLRLAPDIRREFFGDYDLDQLVYQTAEE
                     VQEDLKRSFFYTEWRKAFSTKLKTFGISRVDSPKEESKIFKLFERYCDKVNTNQFLPK
                     IQTPFCSQLFDTPAIERLFTYVPQIIDDLTLKGTFFTYDQFLLELSEFVENGTRQLIS
                     TIEKVDMKALIETYAEALPDGLHFQRRPLPTLPKGLTHVLALDPYKFEAFVGHLLEAQ
                     GYTQLALLGRSGDRGVDVMGLDPKGKSTVVQCKRYIKNNVSSEPIQRLHSFAVTRGAI
                     RKILVTTSDFTPQAKEEARNTDTELINGKKLEQLIAKFMPGIFNNV"
     gene            7285..8592
                     /locus_tag="VI935_02815"
     CDS             7285..8592
                     /locus_tag="VI935_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24565.1"
                     /translation="MNNKGHISILLTPQETFNALRDLTDWADKIYFAYAWASSEGGSA
                     EHWKIIPLAKIFKAIIGNHFAYTEPYVLRKLLKTPNILKVVNDTGGVFHPKVILGVKG
                     NNGFAVLGSSNLTRGGFSCNTELNIKLFGNIKTEPLKDILAFIEKQWNSVRAFCPDDD
                     WIDSYERVYNKRPKPPVVPFKGEKEKNIKFAAELDIPWHQYFKLISEQEGRTCAGGFR
                     IHVFDHVEGSFLEVAEHCQAIFKKYSKFEDISEDDRSYVGGWKPGPSGLFGSMWGAAR
                     FKNIVLEKPDLIGKELNKIPLAGEITQQTIENYFQEMFKFKGINIATATRLLCMKRPD
                     YFIPVNGANKKLIKKIFGYAPTDGESYMKLQNTIIGFPWFQEQEPHGNKDEIRVWRNR
                     VALLGACRNIRLQKAILEETEIRPGFNVHFFKQLLPYDEANCA"
BASE COUNT         2646 a         1748 c         1831 g         2384 t
ORIGIN      
        1 gcctgcgaca gggtggaaaa ataggaatca aatgcggaaa acacgccatc aggcgtcttg
       61 gaaaatgaag tttctcctct tgtctggtga ctcattaaca aactcgcttc cctaaacttg
      121 aattgatcag agattcctta gtacctttct aataattaat ctctatcaga aaaggagggc
      181 cttttatgtt ctatttgggg cagttctcat ttcatgaaga gagagaaaag aatacgtatt
      241 atggattttt taattgcata gtcgaagcag agaatctaga aaaagcggtc gaccttttta
      301 ataaactcct caaaagcaca cgaaaatcag atgatctatt cgatggtgca atcagtattt
      361 tcttagatga gatcttcgaa atagaaagga ttcccaagga cggcgtttta actacttaca
      421 tgagcatccg tggcgacatg cccgcgatgc ttcgctgttc catatttaat gaagcacctg
      481 gtattcagtg ttatggatgg acggaagacc aggatttaga agaggaaaaa gaaaaggctt
      541 caaaggacgc ggtagcgcaa ttgccatttc tcaaatttgc agaaaaagct tgatcagttt
      601 tgtaagcttc ccttaaaagt agacatgtga taagagcttt ctcgcaatat acagccggga
      661 ggttcgacat gaaaaagtca cgtttcacat agtcacagct cgtagcggtg ctcaaggagg
      721 ctggcaattt ctccttccat gcgctttctt atgcggagca tctcttcgac atccgccgag
      781 gtaaggggct tctgatagag cgcctcccag tcgaagcacg ggggcatgga ggcttccgac
      841 cttcggcgca tgaaagagct ggaaacggag aactcgaagc tcaagcgcat gtatgctgac
      901 atggcccttg agaaccgagc attaaaggat ctgatcgaaa aaaagctcta aggccgcccg
      961 agaaacgcga agctgtcact tatctgattg ccgagtataa attgtcggta cagagctgct
     1021 gccgttacat tggcatatcg cgggcggcct attatagaac accggtctac gccgaccgtg
     1081 atgccgagat catcaaggct ctgaacgccc tgatcgaacg gcatccacgg tggggtttct
     1141 ggatgtgcag gaaggtcctg aagcgctctc gaacaccatg gacgccgact tctgcgtggc
     1201 tgcattggag gatgccttgg agcgttacgg gcgccccgag atcttcaaca ccgactaggg
     1261 gagtcagttc acaagctttg cttttaccaa cgcgctcaaa gacgcctcca tccgtatctc
     1321 gatggatggc aaggggcgct ggacggacaa cgtgatgatc gagaggctct ggcgctctct
     1381 caagtatgaa tgcgtgtatc tgaacgccta tgagaccgga agcgctctcc gggagggact
     1441 ctctcgctgg atcagctttt acaacgagcg ccgtcctcac tcaagtctgg acgacaagac
     1501 cccttttgag gcatactggc aaaaacccag ggtcggctac tccagccgtc ccaccgtaca
     1561 ggcggcataa cctctatggc tttccacctt atttacgccg ccaacctgtc cagtcaaccg
     1621 gggccacctc tctgtttcga atgtgaaaac taccttcgac aaacccgttt tatgtaggta
     1681 agtcgttgat gaagaaattg tcgcaaacgc cccccacgag atagccgctt gcgtcgacag
     1741 tgaggttcgt attcgcttca tattattgtc ccgagatggt ataaatgctc actttcctag
     1801 aaattcccac ctgcgccatt ggatttcgag atgcttgaaa aactactgag gaaaagatag
     1861 tatgaaaaaa ttaagacaaa aatgcaatta atcatacaat agatttattg tatgaatgca
     1921 attattttgg cgaaattatg ttacgctcag tatgttgatt ttttcagcat agttgaaaga
     1981 tgagggtatg aaaagtagta aaaataaaac cgcttttaaa cttggcaata tgagcagaag
     2041 tatctcgtta aggatgcaat accttggttc gaaagcacgc atcgtagact ggattttaca
     2101 tgaaattgaa aagcgcctcc cgaagataga tagttttgtt gatgcttttg caggtaccgg
     2161 tgttgtcggt gccgcagcag taaagtgcgg ttaccggcta tacgctaatg atatccagcc
     2221 ttattcatat tgcattctcc aagcaatgtt tgctcaacca actaaaggtc tggataaggc
     2281 cattgctgat ttggatgcca tagccaatgt ctcaatagct tccatctttg gctcaaatat
     2341 cgctgcatat gctgaagaag aagattgttt tttttctgaa tacaagaatg gcaaattaaa
     2401 ctggaaaaaa tatattactt tttgtgaaca aaccccgatc ataactccga caaaagactt
     2461 tattttatct aaaacaaaca ataaagcata tgacctgttc gtaacttatt actctaatac
     2521 ttattttggg gtccgtcagt gcgttcagct cgatctgcta aggaagatag catctcaata
     2581 tccagagaat gttacttacc atataatagc agcaaccctg gcagcaatga cctatgctgt
     2641 ttcaagtaca acgcacttag ctcaatatct gaagccttca tccgcaagta ccgcacagaa
     2701 tttaatcaag aaacggtcta tcaacattat tgctgatgtt gccactaggc tagcagggtt
     2761 aagcaaaacc aaaccaacta caactcctga ttgcatctac aacagtgatt tccgtgttgc
     2821 tcttaattcg atcaagccag gttccaagca cgtggtatac gtagatcctc catattttaa
     2881 ggaacattac tctcgatatt accatgtgct tgacacattt tgtttatacg attacccaat
     2941 gctgacatac aatcctcgca ccgatgcagt gacttctggc cgttatcgcg aagggagaat
     3001 tgtttcggaa ttcggattaa aatcatcggt caaaaaggca ttttctgatc tcttcaaatt
     3061 gggtgtgtca cataggacca acattgtaat aagttacgct gaaacatcac ttgtaggaaa
     3121 gaacgaactc atctcgattg ctaacgccaa tggatacaat gctgacgtta aagaagtcac
     3181 gctaatgcat tcaggccaag gtcaacctag acacaatctt gtaagagaat acttattcat
     3241 ttgcacatta taaaggacct aagttgaaag acgtattaag ccttattaaa agattggaaa
     3301 atatttctcc tgagcacgac acccctgtcg gtttaataca cccgtattgg gcaaggaaac
     3361 ctcttaatgt catccaacag cttgtcagag aattttcaga aaatggtgat attgttgccg
     3421 atcccttcgt tggttccggt acaactgttt tcgcggctct ttctgaaggc aggaaagcaa
     3481 ttgcatcgga tctcaaccca ttggcaattt ttgtaacaaa gtctattgtc gaattactgg
     3541 agtcgccaga agataaactc aaggcagcac accattttgt agaggatttc agtgatgaag
     3601 ttttacccat gttccaatat tgcgaggact attacgtcga aagagaacgg ttcgttgtaa
     3661 aaggagaatt tgaagacggt ggttatgagc ttatttcaac agaagtggtt ctgaaaaaaa
     3721 agactggaaa aagtttgaaa gggaggcgca tcgagaaacc tacctccaat tggacggata
     3781 aaaaaattac agcatcttta tctaatagcc ccattaattt tggatcattg aagctgcgcc
     3841 caaattcaag gattgccatt ccgaatggcg cgactcttgc ccattactat gaatataaaa
     3901 accaaattgc cattaactta gccctccagc tgatagcgtc aaagaagtat ggcagaaaga
     3961 acgagaatac gcttaaactg ctgttgtcag catctttgcc actactacgc ttaagcgaca
     4021 agaaagcgtc ttcacagtgg ccatattggc gtcccaaaga acatctgact tcccggaatc
     4081 ctataccagt actgatgaaa aaattacaat caatcgaaaa agccattaaa tggttaaaga
     4141 caaatgttga aggtaaagac aaatggaagt tatcagatcg ggtccaactt tataatacgt
     4201 ctatccaatc gttagtaccc aaatatttgg aacctgagag agtcgaccta gttctgacag
     4261 acccaccata ctctgatcaa gcaccatatt tggagtattc tgcactttgg attgagttga
     4321 tcgggttaaa actcccctta aatgcttaca aacatgaaat tgtgaagact gatgcaccct
     4381 caagggtagc tgatacagac gattatatcc caagattgcg cgaaggatta aaggcatgcg
     4441 cccacttgct taagccagaa ggtactctca tctggtttta tcaggatcat aatatagaac
     4501 attgggcggc actcgatgaa gaagcgcttg tgaatggttt aaggatcttg gacgttgtgc
     4561 ctctggcgaa gcagcgtcgc tcgatgaaaa cggtgacttc tcccggtgct acgcttgacg
     4621 gcgaccttat tcttatattc aaaaagacaa acgtcgtagc aaaacgcccg catacgactg
     4681 agaaagttcg cagaattatc ctaaatgaat tagcccctca ttccgctagc tctagctact
     4741 ttgaaaaata tgctgcaatt gtgaagtctg gcttgaaatt ccatttgttt gacctactgt
     4801 cccagtcgga taaggatatc cgcaaaatac tatcctctat ggataattgg agatgatatg
     4861 tactggcttt tgagcgagga agaacagaag aaagttcgag gtattgtaaa cacactaatt
     4921 gtcgatttat ctgtcattgt tggcaaccct cagaccgacc agttgtttcc tttcgtaggg
     4981 cggaaggata gacgtaaagc ggaatctata gtaaaacatc tatcgccgaa aaacagcgta
     5041 gttgctgacc cgttttctgg atctggtatt catatatatg ccattttatc ccttggccgt
     5101 gtagccattg ccaatgaatg ggaaccatac gcaaaccgcc tatcaagcgc accgtggtgc
     5161 ctaccacaaa agtcagcttt ggaatttgca tataatgatc tgatcgaaaa aatacaacca
     5221 gaacttgaca agctttactg gacaaaatgt gcttgtgggg ctgatcatgt gttcaaaagt
     5281 cttttttatg atcgtgaacc tttgaaatac acagctgtta ctaatcatga gcgcctgggt
     5341 ccaaatggcg aaaatgtcga atatcgaggc aaatacaaat gcccgaattg cggaagtaga
     5401 gaaaagtttt ttgataaaaa cgacgctaaa catctctcca aattatctac ccttccgaca
     5461 gatcctattt ttgacacgac attaattgaa aactcgcgaa ttaatctttc aaaagcattc
     5521 actgtttatg gtgcattatt cccacatcgg tcaaagttag ccttaagtat tatttggaaa
     5581 aatatccaac agttacaatg tcaatctatt gttaaatctt ttttggaaga tgtctttttg
     5641 tcaatacttc cacaggcaaa atataaggac tataggagta aaagccagga tttacactgc
     5701 cctccagaaa aactacgaga aaacaatttg ttatatgttt atcaagagaa ttatcataaa
     5761 cgctgggagg gactaaagac ttataagttc gctacagctg ataaagcaaa aacagctatc
     5821 aaccctatca aaatgtccga ttaccgtgat tttttcaatt ctttgggtga tgagtccatt
     5881 gatttggtat tcaccgatcc cccttggatt gatgggacag cttacttcga acgtgcccaa
     5941 ttgtaccatc cttggatggg ttacgattta aagaaagaca agctgagact tgaaaaagag
     6001 atggttgttt ctgatgcacc tacccgcaaa gaagtccatg gtattgagcg ctggtgggac
     6061 gacttggaag ttttttttca aagtagctat cgcgtactca aggcaactca attcattgcg
     6121 cttttcttta gacctattcc agctgatcag tggttgagta acctcaatcg cattaaatta
     6181 gtcgcgcgca gagccggatt tgaaccatta ttaagtattg atgttaaaag ctctgatcct
     6241 tcgatgcgaa ttcaacaatc agcttcctac gttttttcca aagatgtagt tatgcttttt
     6301 ttgcgcttag cccctgacat tagaagggag ttttttggag attacgactt agatcaatta
     6361 gtctaccaaa ctgctgaaga agtccaagaa gacctaaaac gctcgttttt ctatactgaa
     6421 tggcgcaagg ctttctctac taagcttaaa acttttggaa taagtagggt cgattccccc
     6481 aaagaagaat ctaaaatttt taagctattt gaaagatatt gcgacaaggt caacacaaat
     6541 caatttctac ccaaaattca gacaccattc tgtagtcaac ttttcgatac tcctgcaatc
     6601 gaacgacttt ttacatatgt tccgcaaata attgacgatc ttacacttaa agggaccttt
     6661 ttcacttatg accaattttt attggaactt tccgagtttg tggaaaatgg gacgcgccaa
     6721 ctaatatcga ccattgaaaa agttgatatg aaggctttaa ttgaaactta cgcggaagct
     6781 ttaccagatg ggttgcattt ccaacgccgc cccctgccca cacttcccaa aggtttaaca
     6841 catgtattgg ctttagatcc ctataaattt gaggcatttg ttgggcattt acttgaagcc
     6901 caaggatata ctcagctggc gctgttaggc cgttccggcg acagaggcgt tgacgttatg
     6961 ggattggacc ctaaaggcaa gagcactgtt gttcagtgta aacgatatat aaagaataat
     7021 gtttcatctg aacccataca aaggctgcat agttttgcag taacaagggg tgccatacga
     7081 aagattttag taacaacatc tgattttaca ccacaagcca aagaagaagc ccgcaatact
     7141 gataccgagc ttataaatgg aaagaaattg gaacagctta tagctaaatt catgcctggt
     7201 attttcaata atgtatgagc actgttatat cagcccagca tatattgagg gacttaaaat
     7261 ccaattttga tgaaagaggt ggacttgaat aataaaggac atatatcgat attattaaca
     7321 cctcaagaga cattcaatgc cttgcgggac ctaacggatt gggctgacaa aatatatttt
     7381 gcatatgcct gggcatcctc tgaaggtggt agcgctgagc attggaaaat tattccttta
     7441 gctaaaattt ttaaggctat tattggtaat cattttgcat atacggaacc atatgttctt
     7501 agaaaactgt taaaaacacc aaatattcta aaggttgtaa atgatacggg tggagtattt
     7561 catcccaagg taattttagg agtaaaaggt aataatggtt ttgcggtgct aggctcaagc
     7621 aatttaacca gaggcggttt ttcctgcaat acggaattaa atataaaatt gtttggaaat
     7681 attaaaaccg aacccttgaa agatatttta gcatttattg aaaaacagtg gaatagtgtt
     7741 cgggcgtttt gtcctgatga tgattggatt gacagttatg agcgtgtata taataaacgg
     7801 ccaaaacctc cagtggttcc ttttaaaggt gagaaggaaa aaaacattaa gttcgcagca
     7861 gaattggaca ttccttggca tcaatatttt aagctcatat ccgaacaaga aggaagaaca
     7921 tgcgcaggcg gttttaggat tcatgtgttt gaccatgtag aaggttcttt tttggaagta
     7981 gcagagcatt gtcaggcgat tttcaaaaag tattcaaaat ttgaagatat atcagaagat
     8041 gatagaagtt atgttggtgg ttggaaacca ggtccgtcag gtctttttgg tagcatgtgg
     8101 ggggccgcac ggttcaaaaa catagtttta gaaaaaccag atttgattgg aaaagagcta
     8161 aataagattc cattagccgg ggaaattact cagcaaacaa tagaaaatta ttttcaagaa
     8221 atgtttaaat ttaaggggat aaacatagct acagctacaa gattattatg tatgaagcgg
     8281 ccagattatt ttattccagt aaatggtgca aacaaaaagc taataaagaa aatatttgga
     8341 tatgcaccga cagatggtga atcttatatg aaacttcaaa ataccatcat tggcttccct
     8401 tggtttcagg aacaggaacc acatggcaat aaggatgaga tcagagtatg gcggaatagg
     8461 gtggcactcc taggagcctg tcggaatatt cgtttgcaaa aagcgattct ggaagagaca
     8521 gagatccgac ccggcttcaa tgttcacttt ttcaaacaac tcttgcctta tgatgaggca
     8581 aattgcgctt agcacgatag ctgccgcct
//
LOCUS       DATDRL010000080         2051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1056829, whole genome shotgun sequence.
ACCESSION   DATDRL010000080 DATDRL010000000
VERSION     DATDRL010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056829"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="VI935_02820"
     CDS             <1..1467
                     /locus_tag="VI935_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24566.1"
                     /translation="LKTPYIRCKVIKKGRFFYYFDEADIQTIRGYDLDFILHFGFGII
                     PREILNVAHYGVWAFHHDDEEKYRGSPSCFWEIYNGDNVNGAILQRLTDRPDVGVILK
                     KGFLRTINHSYGKNIDAVYFESARWPALVCIDIRNGNADYLTAPPSQTKAPIFRAPND
                     LQMLLFAIKLLRNFLAAKYNALFRHVQWNIGIIYEPIHVFLKPDAKPKIHYLQHPKKG
                     KFLADPFGILKNKKLIILCEDFDYRSFKGTISSIELIDGTHPSQPVVAIDLPIHTSYP
                     YLFEYQGEFYCIPDTSRAREISLYKAQKFPHRWIRVATLIRNIAGLDTTVFQYEGLWW
                     LTCADKEQGNFHNLFIWYAPDLLGPWKPHAANPVKTDIRSVRPAGTPFMYNGYLYRPA
                     QDCSRTYGGQIVLNRVIRLTPTEFKEEQAAVIKPYTNSPFPDGVHTVSAVGDITLIDA
                     KRFIFIGSAFKHVLVRWFTKVLRASVQASKSSPQVGQG"
     gene            1654..1812
                     /locus_tag="VI935_02825"
     CDS             1654..1812
                     /locus_tag="VI935_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLE24567.1"
                     /translation="MIWRDIKVRYRQTVLGVAWSILQPFLTMVVFGIFFGQLAKVPSD
                     GHLWLLLG"
     gene            1794..>2051
                     /locus_tag="VI935_02830"
     CDS             1794..>2051
                     /locus_tag="VI935_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLE24568.1"
                     /translation="MATFGLVVGFMVGITSIGSGSVIAIFLLIFSNMKSKDIVGTDIA
                     HAVLLTGVATMGHMGIGIIDLRLTLNLLIGALPGVIMGSYLT"
BASE COUNT          570 a          416 c          436 g          629 t
ORIGIN      
        1 ttaaagaccc cttatatacg ctgcaaggtt attaaaaagg ggaggttttt ttactacttc
       61 gacgaggctg atatacaaac tattcgtgga tatgatctgg atttcatcct gcattttggt
      121 tttggcatca ttcctagaga aatattgaac gtagcccatt acggggtatg ggcatttcac
      181 cacgatgatg aggagaagta cagaggtagt ccctcttgct tctgggaaat ttacaatggt
      241 gacaatgtaa atggagctat actgcaaagg cttactgata gacctgatgt cggtgttatt
      301 cttaaaaagg gctttctaag aacgataaat cactcctatg gcaagaatat tgatgcagtt
      361 tattttgaaa gtgcgcgttg gcctgcgctg gtatgtattg atattcgtaa tggtaatgcc
      421 gattacctaa ctgcccctcc ttcccaaaca aaagcgccta tatttcgtgc tccgaatgac
      481 ctccagatgt tgctgtttgc gattaagctg ttgaggaatt tcctcgctgc caaatataac
      541 gctctgtttc gtcatgtcca gtggaatatt ggaattatat atgagccaat acatgttttt
      601 cttaaacctg atgccaaacc taagattcat tatctacaac accccaaaaa gggtaagttt
      661 ctagctgatc ccttcggaat actaaaaaac aaaaaattaa ttatcctttg tgaagatttc
      721 gattaccgtt cttttaaagg aactatttct agtattgaac ttattgacgg aacccatcct
      781 tctcagcctg tggtcgcaat tgatctaccg attcatacgt cctaccctta cttatttgaa
      841 taccaagggg aattctactg tattcctgac acatctcggg ccagagagat aagcctttat
      901 aaagcccaaa aatttccaca tagatggata agggttgcta cccttatcag aaacattgct
      961 ggccttgata ccactgtctt ccaatatgaa ggactttggt ggcttacgtg tgcagataaa
     1021 gaacagggta attttcacaa tttgtttatc tggtatgcac cagatcttct gggaccgtgg
     1081 aaaccccatg cagccaatcc ggttaaaaca gatattcgtt ctgtccggcc tgcaggcact
     1141 ccctttatgt ataacggcta tctatatcgt ccagcgcaag attgttcaag aacctatgga
     1201 ggacaaattg tgctcaatcg agtgataagg cttacaccca cagaattcaa agaagaacaa
     1261 gcggcagtta ttaaacctta tacaaatagc ccttttccag atggtgtgca caccgtatca
     1321 gctgttggcg atattaccct tatcgatgcg aagcgcttca tatttattgg aagtgctttc
     1381 aaacacgtct tagtcagatg gtttactaag gtattaaggg cttcagtcca agcgagtaaa
     1441 agtagtccgc aagttgggca gggttagaaa accctgcctg ggaaaactcg atgcactctc
     1501 ctgtaaatct tggaaatcct tttgaactgt cagtcttgga catttgaggc aatcatggtt
     1561 gagacgctgc aatctttaat catccgccca tcacatggtt gggtatcact caggctcaaa
     1621 gacctgtggg aataccgtga gctgctctat ttcatgatat ggcgtgacat taaagtacgt
     1681 tacaggcaga cagtactcgg tgtagcctgg tcaattctgc aaccttttct caccatggtc
     1741 gtattcggta tcttcttcgg acaattagct aaagtgccat ctgatgggca cttgtggcta
     1801 cttttgggtt agttgttggt ttcatggttg gtataacatc aatagggagt ggttcagtaa
     1861 tagctatatt tttgttaata ttctcaaata tgaaatcaaa agatatagtg ggtacagaca
     1921 tagcacatgc agttttacta actggtgttg caacgatggg tcacatgggc ataggtatta
     1981 tagaccttag gttgactctt aatctgttaa tcggcgcttt gcctggtgtt ataatgggta
     2041 gctatctaac t
//
LOCUS       DATDRL010000081         2434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3487082, whole genome shotgun sequence.
ACCESSION   DATDRL010000081 DATDRL010000000
VERSION     DATDRL010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3487082"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VI935_02835"
     CDS             complement(<1..874)
                     /locus_tag="VI935_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24569.1"
                     /translation="MKTKWVSTRNFDAGNYLENFLVAAVAAVLGIRLFLKMTGYPQIG
                     GSSLHIAHMLWGGLLMLVSIIILLSFLNRASERLASIVGGIGFGTFIDEVGKFVTRDN
                     NYFFEPSVAIIYVTFILIFIAIRAIQIGRSYSQIEYLLNALRETQEVVLHDLDEEGKN
                     KALYYLSKSDPSDPLTTSLKNLLSGTKLVPSVRPGLYTRIKLYFRDLYYKITKSRAFS
                     ITIVIFFLGQLLVTLAYVIVLVFFVGFGWDKILNLRIFDHVAQRIVGLSFIQWAELFS
                     SLLSGVFVFLGVFYI"
     gene            1023..1367
                     /locus_tag="VI935_02840"
     CDS             1023..1367
                     /locus_tag="VI935_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017747812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HLE24570.1"
                     /translation="MMILIVNWFLSALSLIIVAHVVPGFEIKGFGTALIAAIVIGLVN
                     ATIGLILKILTFPVTILTFGLFLFVINALMLRFATLFVSGFVVKGFLPAFLGAIVLAI
                     VNTALRHLVFKY"
     gene            1537..1755
                     /locus_tag="VI935_02845"
     CDS             1537..1755
                     /locus_tag="VI935_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24571.1"
                     /translation="MSETCVRTTLSTFPYGNIQKCSCGGYYVTFGYVTLRLSHEELKS
                     FTEMLNNVIGGEELIRENPQDITNGGYR"
     gene            1994..>2434
                     /locus_tag="VI935_02850"
     CDS             1994..>2434
                     /locus_tag="VI935_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24572.1"
                     /translation="MKLKNNNRCVWSKILTLALTWLVFLFSLEGLAQDIPKNKSTQEE
                     RLEELEREVKELKEEIRRMKGDKGVIEGKEGKKIEEKEPETVTKMPADVEKKEGKGVL
                     EYLAERVRLGGYGSFRYEYLTAAHEDLNTFTLRRLVLTADARPVD"
BASE COUNT          809 a          405 c          499 g          721 t
ORIGIN      
        1 ttatgtaaaa tacgccgaga aatacaaata caccggataa aagcgatgaa aaaagctcag
       61 cccattgaat gaaggagaga ccaactattc gttgagctac atgatcgaag attcgaagat
      121 tgagtatttt atcccaacca aaccctacaa aaaacaccag cacgattaca tatgctaacg
      181 tgaccaaaag ttgacctaaa aagaaaataa ctatagtaat agaaaatgcc cttgacttag
      241 tgattttata atataggtct cgaaaatata gtttgatgcg ggtataaagc cctggtctta
      301 cggatgggac caattttgtg ccagaaagta gatttttaag tgatgtcgtt aatgggtcag
      361 agggatcgct tttacttaaa taataaagcg ccttattttt cccctcttca tcaaggtcgt
      421 gcaacactac ttcttgcgtt tctcgtaaag cgttcagaag atattctatt tgggaataac
      481 tgcgaccaat ttgtatagcg cgtattgcga taaaaattaa tataaaagta acgtagatta
      541 tggcaactga aggttcaaaa aaataattat tatctcttgt cacaaatttc ccaacttcat
      601 ctataaaggt gccgaatcca attcctccaa caattgaggc caaacgctct gaggctctat
      661 ttaaaaagga taggaggatt attatagata ccaacattaa tagcccaccc cagagcatat
      721 gtgcgatatg tagactactg ccacctattt gaggataacc agtcatctta aggaaaagac
      781 gaatccccaa taccgctgca acagcagcga caagaaaatt ttccaagtaa ttcccagcat
      841 cgaaattgcg agtggaaacc cacttcgttt tcatatgtgc ttaattataa accgtttcta
      901 agtacaacat ttaacttttc taaaaaattc tcaattatat ttaatcggaa gaaacattac
      961 agaatatagt atattaatat aagctatgtg gtagtgtaat atttagggag gaagcttcag
     1021 ctatgatgat tttgatagtg aattggtttt taagcgcatt aagcttgatt attgtcgcgc
     1081 atgttgtacc aggattcgaa ataaagggat tcgggacggc gttgatagct gcgatcgtaa
     1141 tagggttagt caatgctaca attggactaa tcctaaagat tttgacattc cctgttacaa
     1201 tacttacctt tgggctattt ctatttgtga taaacgcact tatgttgaga tttgcaacat
     1261 tgttcgtgtc tggctttgtc gttaagggat tcctgccagc tttcttaggc gctattgtac
     1321 ttgcaattgt aaacacggcc ctacgtcacc tggtttttaa gtactaagta tactaggttg
     1381 taatacgtca gcgtttttat ggtaaaatgt ataatcaaat aatatggatt gagttttgtt
     1441 ctaggctttt tttatgcggt ttagcttatt gatgaaatct ttaaagaact agagcagtgc
     1501 taatgcacaa tatgactaat aaaaaagggg attaatatga gcgaaacatg tgttagaaca
     1561 acactgagca catttccgta tggtaacatc cagaagtgta gttgtggtgg ttattacgtt
     1621 acctttggtt atgtaactct acgcctaagc cacgaagagt taaaatcatt cacggagatg
     1681 ttaaataacg tgattggagg cgaagagctc ataagagaga acccgcagga tataaccaac
     1741 ggtgggtata gataagactg tagttgattt taactctgtt cattaaaact aaagggcacg
     1801 acccagcggg tgtagattaa ggccatttaa acaaaaccaa cacctagtct ttgacttcta
     1861 tctgaaagaa gtagtttttt cccctccatt ttgaaagcca ataagattta tttagttcct
     1921 cttgacaact aatcctatcc tgttattttg attatgaaaa taaatttcaa tattaaaaaa
     1981 ggaggataaa ggtatgaagc taaaaaataa taataggtgt gtatggtcaa agatacttac
     2041 tttagcactt acatggcttg tctttctttt ttctttagag ggattagctc aagatatacc
     2101 caagaataaa tccacacaag aggagcgttt agaggagttg gagagagagg ttaaggagct
     2161 caaagaggag atcaggcgta tgaaagggga taagggagta attgagggga aagaaggtaa
     2221 aaagattgaa gagaaagaac ctgaaactgt cactaagatg cctgctgacg ttgaaaaaaa
     2281 ggaaggtaaa ggagtacttg agtatttagc tgagagggtt agattggggg gttatgggtc
     2341 ttttcgatac gagtacttaa ctgcagccca tgaagattta aataccttta cactccgccg
     2401 tcttgttctt acagccgatg caagacccgt agat
//
LOCUS       DATDRL010000082        11073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2398353, whole genome shotgun sequence.
ACCESSION   DATDRL010000082 DATDRL010000000
VERSION     DATDRL010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11073
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2398353"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            394..469
                     /locus_tag="VI935_02855"
     tRNA            394..469
                     /locus_tag="VI935_02855"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:427..429,aa:Ala,seq:ggc)
     gene            1328..2113
                     /locus_tag="VI935_02860"
     CDS             1328..2113
                     /locus_tag="VI935_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24573.1"
                     /translation="MHYYENIPIIMGVLAFLTAIVATIYSWRLIIQLKEKQKVLQGFT
                     EGQKQAMIDIIQSLSNPEIRGLANIGQIKEAISKLEKSFPGEMDNLPENLLNDYIDIL
                     LLSKEESKWQKAWELLQKQKSPFLKSYISLSYLFWSIGRINDAIKVSEEGLKFAEMTG
                     SQEALDLVRLKNNLAYYYADSGDPKYKTQAEIYISEVLNQDPENPLHIDTAGYIKIVF
                     GNTKSEILQGVNLCEDARRKGSSFEIYIKHVNKAKQRLSQLEF"
     gene            2400..3293
                     /locus_tag="VI935_02865"
     CDS             2400..3293
                     /locus_tag="VI935_02865"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HLE24574.1"
                     /translation="MNLVQIGSLTETQAREYLEKLRWPNGVECPHCNSQEVTKLAGHK
                     HREGVYQCNTCHKQFTFTVGTVMEDSHIPLKKWLMAFHLMCSSKKGVSAKQLQRDLGL
                     SSYETAWFMAHRVRYAMSNAPMQEKLKGRVEVDETYIGGKPRKGSSKSKRGRGTKKAP
                     VLVLVEKDGNAVSKPIKRVSAKELKSTIRENVDLESRIITDEFKSYKGLNKVFLGGHQ
                     AINHAKGEYSVDGVDTNTAESFFALLKRGHYGIFHQLSKDHLHRYCDEFTFRWNYRKI
                     SDSDKRDIAITQASGKRLMYD"
     gene            3461..3805
                     /locus_tag="VI935_02870"
     CDS             3461..3805
                     /locus_tag="VI935_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24575.1"
                     /translation="MPEKKEIVDFTFTIKPIYQQDSHPIWINYANFFRIADNFILDVG
                     YPDLQKVAELKTKYPDGNIPEGEMQIEMKILSRFALSTTTFLELRHNIEEFYQRAVAL
                     GAIPKQEEQSHE"
     gene            3798..4352
                     /locus_tag="VI935_02875"
     CDS             3798..4352
                     /locus_tag="VI935_02875"
                     /inference="COORDINATES: protein motif:HMM:NF039192.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HLE24576.1"
                     /translation="MNKINFSLDLDVSRRGILAKGILTGKQHQSSIQLETDMASPNLV
                     IEGSYILSPGFTLDSKISRSYVISSFAIPGNVSVASFDNLFLPASNNFFVSIFNSLSV
                     SISDNLSAFRKEQQAFQAMREELKKEYAGKYVAIHGGRVVDSNTDESALIGRFYKTHG
                     NVSVYIDKIGEKRVIKMRSPRRIK"
     gene            4354..4728
                     /locus_tag="VI935_02880"
     CDS             4354..4728
                     /locus_tag="VI935_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24577.1"
                     /translation="MYNYSHNVILPAPVLPIEISTPFTPDLRLQKIALIDSGADISGL
                     PPSVIDELSLNPIREEQIQGSTGIDIRPIYAVNILFHGTSFSNLSVMGMSGEDFIVIG
                     RDILNSFHICLDGFNEVATIKD"
     gene            complement(5036..5623)
                     /locus_tag="VI935_02885"
     CDS             complement(5036..5623)
                     /locus_tag="VI935_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HLE24578.1"
                     /translation="MLTENNQDERILTPEEEVRLLNFGSPELRAILVCALNTGMRKSE
                     ILTLKGSNVDLESNVITLEHTNTKSKKTRRIPINSVLRKLLLEQKLKGGGSEYVFLSS
                     NGNPYKRHDSLKQAFNGALRRAEIKGLRFHDLRHTAATRMIESGASIVAVCRILGHAD
                     LKTTMRYAHPEDSLKEAVECLANFKKIINQKEIHN"
     gene            complement(5811..6113)
                     /locus_tag="VI935_02890"
     CDS             complement(5811..6113)
                     /locus_tag="VI935_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24579.1"
                     /translation="MGVFKRWRKSQDGSNTAYWYIRYPANGKIKWESVGKIGEITKTV
                     AQTKLEERRRQVRLEQLDIIGATIPTLSEFAEKYLRYVRDIKRNRSLMTTLHYRNT"
     gene            complement(6116..6322)
                     /locus_tag="VI935_02895"
     CDS             complement(6116..6322)
                     /locus_tag="VI935_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24580.1"
                     /translation="MNPKRFRPKLIHVYLHTEEDKFVKRHSERNYLSVSELIRGWIHE
                     VMKREGYEIKDPSLPETKPKKGGK"
     gene            6418..7485
                     /locus_tag="VI935_02900"
     CDS             6418..7485
                     /locus_tag="VI935_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24581.1"
                     /translation="MPIKAGMVDEMDEYKNILSRLLRTENHVTEFLCGLCVYKPFREI
                     LVRLFTGGQYGSDDISLDDIDTQTTIEGSIPDLSMLGEKVNILVEVKIDSWRELTQNQ
                     PETYLRWLASSNAESRFFIALIPPVYDYLEELESRINSFHRTNKRNPVNTSIIRWDDL
                     LQKISKDELDQMSPYIKDFSNLLKSRYEVPIIKFTFEEMQSMYDPNSAKGIEKLLKLI
                     EEVLTGLEKKGYQVSHAFNRRWWDDGEYGGYVLFHGQEVLWFGLWQEYWENKGVPLSF
                     GIHFEKWDNRLRSEFKRLHPSSVIFPQGGPNRFLIEKIDRDVFLSDDPGKKILEILEK
                     DLTSLCKQIREKGDQNKNPMK"
     gene            7930..8085
                     /locus_tag="VI935_02905"
     CDS             7930..8085
                     /locus_tag="VI935_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24582.1"
                     /translation="MAEKITDKAKRRVRLLEFFEKYGLRATEEAFGVKKSSIYKWRKL
                     LRDNGGG"
     gene            8091..8771
                     /locus_tag="VI935_02910"
     CDS             8091..8771
                     /locus_tag="VI935_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24583.1"
                     /translation="MCDRSSAPRRRRKAGYDDRVVKFIREVREKYPRMGKTKIKVFLD
                     EYCHRQGIRAVSESTIGRVIKSHRFFSHPRGVSHFGKIKTTRYRKKLRRRGYKAKYPG
                     DLVQADSIVRFRDGINRYIITAIDLVSGFACAYGYTSLSSKSALDFFEKLEGVAPFKV
                     SAIQTDNGSEFEKHFREHLQKSKIKHFWNYPKRPQLNAKVERFNRTVQEEFVEWNWRS
                     LFHEPRVI"
     gene            complement(8924..10069)
                     /locus_tag="VI935_02915"
     CDS             complement(8924..10069)
                     /locus_tag="VI935_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24584.1"
                     /translation="MRNLIIFFVVLSTTFFVLLLLQTLAQPKDVQGWNKARWGMTDED
                     IMKAFNEKVERLPDSKKGRPLSLSASDKKMLEKLARELKEKFEPYHTNLCIRTADIYG
                     KKFEVHFNMNDKTNKLENVNIRPKEKPSKALFKFLEQKLIEEYGFLTRGVTISDTKPP
                     IQMQIRNRFWIFPTTTIELNYAVIKESPSLDIYYYPTKEKDKSIARGKQGKQIEFDFR
                     KTKWGMTKEQVKATESVKLTGATDTLLNYSSEIAGMNATIGFSFSEDKLYRASYAFNE
                     EHTNKNNYISDYENVKEILTREYGSPKSDSSEWRNDLYENDPQYWGLAISLGHVVYLT
                     TWETETTEILLILGGDNYKISFGVVYNSKNLKYLGVEQELEKEEGQF"
     gene            complement(10187..10813)
                     /locus_tag="VI935_02920"
     CDS             complement(10187..10813)
                     /locus_tag="VI935_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24585.1"
                     /translation="MGRIKQNQLDYNFKIHYLINGDEFLKLVKKDPYLIYYNYNDRYA
                     IERLQETCRNGNKKDVNLAKKILIKAGLPAFIPKQSQKRTTENQYLKYWKAHPFEFVQ
                     GVEELKTKLQPLLKQHYKQIEREDKRKDIKNCYKQIFGEDLTDNEVKLLTKGECDKDL
                     TTIALSILSYRMDIPFESLKKSFYKASQKEKLRRRNIVLIVMRNMVKD"
BASE COUNT         3526 a         1800 c         2084 g         3663 t
ORIGIN      
        1 ttctctcggg gtctatatca gcaatcctct ttaactggat tctggcgccg agcctatctt
       61 caatgatgtc cttattttca tttagaactt ttataactcc gcagcctacg gttcctgctc
      121 caattaaccc aacgttaatc atatccataa gtggttctcc ttatttataa aacgaccttt
      181 agagttacag catttcatag taaaggttaa aaaagtatac attattttag gtttgacaat
      241 aaattaatct tggctaaatt atggtattat ttgatgttgt tttatctcta tcgcagcttg
      301 attccatgcg cagtgtgcat ggaatggtaa taagcaatga tagagatggc aataaagagg
      361 atgcgcatca aatggaaatc cgaagcataa tgtggggccg tagctcagtt gggagagcgc
      421 ttgaatggca ttcaagaggt cgagggttca aatcccttcg gctccaccaa tgatttcaag
      481 aagttacaac acatcattat ctctccaatt tttcattgct acggaaattg ctacggttca
      541 gggtgaaatt tctcaaattt ttactacaaa ttattaaaaa tcttccgagt gtgcttatca
      601 tctcgtaata atagataatg ctaaattacc atggttttac ctattcaagt ccctagtatc
      661 aatcaataat ttttggggat tcaacactcg tatatttgta tcaatcttta gtatttgttg
      721 atttgtaatt tatattaata taaaatacaa aatattgggt gcaagtttat agattcattt
      781 attccctttt tcagtatagc gagaaacaag tctggtataa ttttatactt taaatgggtt
      841 aattcccctg aacaatccac actatttttt atcttttccc agctacactc atatcacttt
      901 atttataatg agcgaaatgt tagttttttc aaacgctttc tatcacaaat gtaatgttaa
      961 caaaaatcat cctgtggatt ttaacacgta tagcctttcc tctgttacca ttatggttgc
     1021 aaatcttcct ttataaggta ggtaagattg ggaacttttt ctttccgaat gaaacaattt
     1081 taatttttga tcttgttttc ggtgggatat taatagtaga tatggaaaat aagatattaa
     1141 gaggctgttt gatatttata tatattttta gtgttgtatt gtttattatg tcagcttacg
     1201 caaataagga gagtaatcca aatgttaata tatactggat aggactattg tttatggcag
     1261 taatgattct cattgctttg ctcacagaga tcatcaaaat taagaaacaa aagggaatta
     1321 gagattgatg cattactacg aaaatattcc catcattatg ggcgtattgg cttttttaac
     1381 tgcaatagtg gccacaatat attcatggcg tttaataatt caattaaagg aaaaacaaaa
     1441 agtattacag ggtttcacgg aaggacaaaa acaagcgatg attgatatta tccaaagctt
     1501 aagtaaccca gaaataagag ggcttgcaaa tattgggcaa atcaaagagg ctatatcgaa
     1561 attggagaaa tcatttccag gggaaatgga taacttaccc gaaaatttac taaatgatta
     1621 tatcgatatt ttgttacttt caaaggaaga atcaaaatgg caaaaggcat gggagttgct
     1681 ccaaaaacaa aaatctccat tccttaagtc ttatatttcc ttatcttatc tgttctggtc
     1741 tataggtaga attaatgatg ctataaaagt atcagaggag gggttaaaat tcgctgagat
     1801 gactggctca caggaagctc tggatttagt gagattaaaa aataacttag cttattatta
     1861 tgcggatagt ggagacccca aatataaaac acaggctgaa atatatatat ctgaagtgct
     1921 taaccaagac ccggaaaatc ctttacatat agatacagca gggtatatca aaatagtatt
     1981 tggaaataca aaatcagaaa ttttacaggg tgtaaatcta tgtgaagatg cgagaagaaa
     2041 aggctcttct tttgaaattt atattaaaca tgttaataaa gccaaacagc gtctttctca
     2101 gttggaattt tgacggttat tacctgtatt atctggggtg tggtccccaa gaataggtat
     2161 cgagtaaaat tttggttgaa tttgaagttt tcatattgca cctcttattt gtttttaatc
     2221 tggtatgata ttttataaca tttatattat acattttcaa gagctaaatg aaaagtaatt
     2281 tttattgaaa tttgtatcta aaatatactt aataaaattt aacggaatta tccccttgac
     2341 aaagtaaata taaggtagta tattttatat atatcttaat tagttaaggg agtcaaacaa
     2401 tgaatttagt tcaaattggt tcattaaccg aaacccaagc aagagaatat cttgagaaat
     2461 tgagatggcc taatggggtt gaatgtcctc attgtaacag tcaagaagtg acaaaattag
     2521 caggccacaa gcatcgagag ggtgtatatc aatgcaacac ttgtcataag caatttacct
     2581 tcacagttgg aactgtgatg gaagatagtc atataccctt gaagaaatgg cttatggctt
     2641 ttcatttgat gtgttctagt aaaaaggggg tatcagcaaa acagttacaa agagatttag
     2701 ggctaagctc ttatgaaact gcttggttta tggctcacag agtaagatat gcaatgtcta
     2761 atgcgccgat gcaggagaaa ttgaaaggaa gagtcgaagt tgatgaaacg tacattggtg
     2821 gtaagccaag aaaaggtagt tctaaatcta aaagaggtag aggaaccaaa aaagcaccag
     2881 tcctggtctt ggtagaaaaa gacggtaatg ccgtatctaa acctattaaa agagtatctg
     2941 ccaaagaact aaaatctacg attcgtgaaa atgtggattt agaatctaga atcatcactg
     3001 acgaatttaa atcttacaaa ggtttaaata aggtattttt gggagggcat caagctataa
     3061 accatgctaa aggagaatat tcggtagacg gagttgatac caatacggct gaatcattct
     3121 ttgctttatt aaaacgtggg cattacggta tctttcacca actatctaaa gaccatctcc
     3181 acaggtattg cgatgagttc acttttagat ggaattatag gaaaatatct gattcagata
     3241 aaagggatat tgctattaca caagcatctg gaaagcgttt aatgtacgat tgacttaaaa
     3301 attccagaaa taaataacct aaataaacca cattacctac taggtaatgt ggtttgatat
     3361 ggtgtatact atatacgata tgtttgcatt cgtacataaa aagacttgac acttggaagg
     3421 gaacatgata gaaaggtaag ggtggaaaaa ggagtaatat atgcctgaaa agaaagagat
     3481 agttgatttt acttttacta taaagccgat ttatcaacaa gacagtcatc ctatctggat
     3541 taattatgct aattttttca ggatagcaga taactttatt ttagatgtag gatatcctga
     3601 cttacaaaag gtggcagagt taaaaaccaa atatccagat ggtaatattc ccgaagggga
     3661 aatgcaaatt gaaatgaaaa tattatcgag atttgctcta agtacaacta cttttctaga
     3721 attgagacat aatattgaag agttttatca aagagcagtt gcattaggtg ctataccaaa
     3781 gcaagaggag caaagccatg aataagatta acttctcttt agacttagat gtaagcagaa
     3841 gaggtatact tgccaaggga atactaactg gcaaacaaca tcagagtagc attcagctag
     3901 agactgatat ggcttcgcct aatttagtta ttgaaggttc atatatactg tcccctggat
     3961 ttacactaga tagtaaaata tctaggagct atgtaatttc atcctttgct atccctggta
     4021 atgtatctgt tgctagcttt gataatttat tccttcctgc ctctaacaat ttttttgttt
     4081 cgatctttaa tagtttatct gtttctatct ctgataattt aagtgctttt agaaaggaac
     4141 aacaagcatt tcaagctatg agagaagaat tgaagaaaga atatgcaggg aaatatgtag
     4201 caattcatgg aggccgtgta gttgattcca atacggatga atctgcatta atcggcaggt
     4261 tctataaaac acacgggaat gtttctgttt atatagacaa aattggagag aagagagtta
     4321 taaaaatgcg gtctccacgc agaataaaat gaaatgtata attactcaca taatgttata
     4381 ctgccagccc cagttttgcc tatagaaatc agtacaccat ttactcctga cttgagattg
     4441 caaaaaattg cccttattga ttcaggggcg gatataagtg gtttaccacc ttcagtgatt
     4501 gacgaactta gcttgaatcc cattagggaa gaacaaatac aaggttctac aggtatagac
     4561 atacgcccta tttatgcggt gaatattcta tttcatggaa cttctttctc taatctttct
     4621 gttatgggaa tgtctggaga ggattttata gtaattggaa gagatatact aaatagtttt
     4681 catatttgtc tcgatggatt caatgaagtt gcaactatca aggattgatc tttcaataaa
     4741 aaggaataag agtattaaaa gactgaaacc catatcttta tatcctcttt cgatggtaga
     4801 agctcttgaa gcctttttaa aaaccccgcc attatttaag aaaaaaacaa gggtaaaaac
     4861 agccaaacgt gtcaagaaaa ataataggat ttgaataaat ttagaaccta ctgtgtcaaa
     4921 gggatagttc cgaaatttaa ttaacacagt atgggcaatt aactgtaatg ggttgaacaa
     4981 accagaatga taactaccta agtttcacac tttgattttt tatcaaactc atgctctaat
     5041 tgtgaatctc tttttgatta ataatctttt tgaaattcgc caagcattct acagcttcct
     5101 taagagaatc ctctgggtgt gcatacctca tagttgtctt taaatcagcg tgtccaagga
     5161 ttctacatac tgctacaata ctcgctccgc tctcgatcat cctggttgcc gctgtgtgtc
     5221 tcaggtcatg aaacctgagt ccttttatct ctgcacgtct taaagctccg ttgaaagcct
     5281 gtttaagtga gtcatgtctt ttgtaaggat tgccattaga acttaagaac acatattcag
     5341 agccaccacc ttttagcttt tgctctaaca ggagttttct taaaactgag tttataggta
     5401 ttcgcctagt cttcttgctt tttgtgtttg tatgctcaag agtaataacg ttactttcta
     5461 ggtcaacgtt gctcccttta agggttagta tttcgctttt tctcatacca gtgttaagag
     5521 cacaaacaag aatagcccta agttcaggag aaccgaagtt taacagtctt acttcctctt
     5581 ctggtgtaag tattcgctca tcttggttgt tttctgttaa caattttgct tctgatacgg
     5641 ggttattacc gaagaactct ttttcacgct tggcaaggtt aaacagatga ctaagacagg
     5701 ctaactctcg gttgacggta gcaggtttta cttctttgag tctagcagtt tgtaattatc
     5761 tacgtccttt gaggttattt gtgatagttt cttgactcca tagaactgtt ttaggtgttt
     5821 cggtagtgca aagtagtcat caatgaacgg tttcgcttta tgtctctcac atacctcagg
     5881 tatttctctg caaactcgga tagggtaggg attgtagctc ctattatgtc caactgttct
     5941 aaccttacct gacgccttct ttcttcaagt tttgtctgag ctacggtctt tgttatctct
     6001 cctatcttac ctacggactc ccacttaatc tttccattag ccggatatcg gatataccag
     6061 taagcggtat ttgaaccgtc ctggctcttt ctccatcgtt taaatactcc cataattatt
     6121 tacctccctt ttttggtttt gtttcaggta gggaaggatc tttaatctca tatccttccc
     6181 ttttcattac ttcgtgaatc cagcctcgga ttagctcact aactgagaga taatttcttt
     6241 ctgaatgccg tttaacaaat ttatcctctt cagtgtgaag atatacgtga atcagctttg
     6301 gtctaaacct ttttggattc atgtatatat attatataat caaattacag gtgtcaagga
     6361 tctttgttga caaaagcatt gtaaaacttt atatgtatta ttctttttga tgggtcaatg
     6421 cctattaaag ctggtatggt ggatgaaatg gatgaataca agaatatcct ctcacgtttg
     6481 ttaagaacgg aaaatcatgt tacggaattt ctttgtggtc tgtgtgttta caaaccattt
     6541 cgtgaaattt tggtcagact ttttacaggt ggacaatacg gatctgacga tattagtttg
     6601 gatgatattg acacacaaac aactatagaa ggttccattc cggatttgtc tatgctgggt
     6661 gaaaaggtaa atattttggt tgaagttaaa atagactcat ggagagaatt aacacagaac
     6721 caacccgaaa cctaccttag gtggctagct tcaagcaatg ctgaatcaag attcttcatt
     6781 gctctcatac cgcctgtcta tgattaccta gaagaactcg aatcaaggat taactcattc
     6841 caccgcacta ataaaaggaa ccctgtgaac acatctatta taagatggga cgatttactt
     6901 caaaaaatat ctaaagatga actagaccaa atgagcccat atatcaagga cttttctaat
     6961 cttcttaagt ctcggtacga agtcccgatt ataaaattca cttttgagga gatgcaatca
     7021 atgtatgatc caaattctgc aaaaggtata gaaaagcttt taaaactaat tgaggaagtt
     7081 ttaacaggcc tagaaaagaa aggttatcaa gttagccatg cttttaatag acgttggtgg
     7141 gatgacggag agtacggtgg ctatgtcctc tttcatggac aagaagttct ttggtttgga
     7201 ctttggcaag aatattggga aaataaaggc gtacccctta gtttcggtat tcattttgaa
     7261 aagtgggata atcgtcttag gtctgaattc aaaagactcc atcctagctc tgtaattttt
     7321 cctcagggag gtccaaatcg cttcctaatt gagaaaattg atagggatgt ttttttgtct
     7381 gatgatccag gtaaaaaaat actcgaaatc ttagaaaaag atttgactag tctgtgtaaa
     7441 caaatcagag aaaagggcga tcaaaataag aacccgatga aatgagtcta ataaccacag
     7501 attaatcctt ttcatatgaa tatcatacga tttttataat atcatcatga taatgatgtt
     7561 tattgttctc atgatactca tcaaattttg ttcagtttac gatcatatct ttttatctca
     7621 acagaagatg gtgtggttag attaaagaaa tgtggataat acttgggaac cccaataagt
     7681 aaaaagccga catccacttt tattgtgttg agttgattct cataaaagtg aaggtatttg
     7741 aggaactccg tacaaaagtt tatgaatcga aacccagtct catagttctc aatcaactga
     7801 ttaatttatt taggggttgg tgcaaaagtt cagaaacata atggaaaact ctaagagtag
     7861 ataatgagag tttttcgagt atatcagggt atcccaggct ttgtcaggtt gtacaagtat
     7921 tctgttatta tggcagagaa gataactgac aaagcaaaga ggagagtaag gttattggag
     7981 ttttttgaga aatatgggct tagggcaaca gaggaggcat ttggggtaaa gaagtcatcg
     8041 atctacaagt ggaggaagct tctaagggat aatggaggag gatagaggtt ttgtgtgata
     8101 ggtcgagtgc accaagaagg aggaggaaag cggggtatga tgatagggta gtaaagttta
     8161 tcagagaggt aagagagaaa tacccccgga tgggaaaaac aaagattaaa gtgtttttag
     8221 atgagtattg tcatagacag ggcataagag cggtatcaga atcaacgata ggtagggtaa
     8281 taaagtcaca tagattcttc agtcacccaa gaggggtaag ccattttggt aagataaaga
     8341 cgaccagata caggaagaag ttaagaaggc gtgggtataa agctaaatat cctggagacc
     8401 tggtgcaagc ggattctatt gtgaggttta gggatggtat aaatagatat atcataacgg
     8461 caattgacct ggtctctggg tttgcctgtg cctatggata cacaagccta tcgtctaagt
     8521 cggccttaga tttctttgag aagctagagg gggttgctcc atttaaggta agtgcaatac
     8581 agacagacaa cggatctgag ttcgagaaac acttcaggga acatttacag aagagtaaaa
     8641 taaagcattt ttggaattat ccaaagcgtc cacagctaaa cgctaaagtg gagcggttta
     8701 atagaacagt gcaggaggag tttgttgaat ggaattggag gtcattgttt catgaaccta
     8761 gagttattta attcttcact tgctttttga taataatgtt tggttatgaa gaaagtgaaa
     8821 attaccccta aaataaacgt tataagccct acaacactat taaatattgt attccaattc
     8881 ataatattca taaacttaaa ttaagaatta cagtctgatt aaattaaaac tgtccttctt
     8941 ctttttccag ttcttgttcc actcccaaat atttgaggtt cttactgtta tacacaaccc
     9001 caaaagaaat tttgtaattg tcaccaccca aaatcaaaag tatttcggtg gtttcggttt
     9061 cccatgttgt taagtagact acatgaccta gactaatagc aagaccccaa tattgagggt
     9121 cattttcgta taagtcgtta cgccattccg aactatcact ctttggagaa ccatattctc
     9181 tagtcaaaat ttcttttaca ttttcatagt ctgaaatgta attattttta tttgtgtgtt
     9241 cctcattgaa agcataggaa gccctataga gtttgtcttc agaaaaagaa aagccaattg
     9301 tagcattcat accagcaatt tcgctactat aattgagcag tgtatctgta gcccctgtca
     9361 acttgacgga ttcagttgcc ttgacctgtt cctttgtcat accccacttt gttttgcgaa
     9421 aatcaaattc gatttgttta ccctgtttcc ctctagcaat cgacttatcc ttttctttag
     9481 tgggataata ataaatatcc aagctgggag attccttaat gacggcataa ttaagttcta
     9541 ttgtggttgt tggaaatatc cagaaacggt tgcgtatctg catctgaatt ggtggtttag
     9601 tatcactaat agtaactcca cgtgtgagaa agccatactc ttctattagt ttttgctcca
     9661 agaatttaaa tagggctttt gagggttttt cttttggacg tatattaaca ttttctaatt
     9721 tatttgtttt atcattcata ttaaaatgaa cttcaaattt tttaccataa atatccgctg
     9781 ttcttataca caaattagta tggtaaggtt cgaatttttc tttcaactct ctggcgagct
     9841 tttctaacat ttttttatca gaagctgaga gtgagagggg tcttcccttt ttagagtcag
     9901 gtaagcgttc taccttttca ttaaaagctt tcataatatc ttcgtctgtc attccccatc
     9961 gggctttgtt ccatccctgt acgtccttag gctgagctaa agtttgaagt agtaataaaa
    10021 caaaaaaagt tgttgataga actacaaaaa atatgattaa atttctcaat gttacacacc
    10081 ctgaaatatg tgtttatgtc tttaatgttc tacttattga gaaaataaca tgttttattg
    10141 cgtcctggta agtgaagttt taaatatcat ttctttggta gtgatattag tccttcacca
    10201 tatttctcat aacaataaga acaatatttc ttcgcctcaa cttttccttt tgggaagctt
    10261 tgtagaaaga tttttttagt gactcaaaag gaatgtccat tcgatatgat aatattgata
    10321 atgcaattgt agtcaggtct ttatcacatt ccccctttgt taatagcttt acctcattat
    10381 cagttaaatc ctccccgaaa atttgtttat aacaattttt aatatctttt cttttatctt
    10441 ccctttcaat ctgtttataa tgctgtttca ataaaggttg aagttttgtt tttaattcct
    10501 caactccttg aacaaactca aatggatggg ctttccagta tttcaagtat tgattttcag
    10561 tggttcgttt ttgtgactgt ttgggaatga atgctgggag accggctttg attaaaattt
    10621 tcttggctag attaacatcc tttttgtttc cgttacgaca agtctcttgt aacctttcta
    10681 ttgcgtatct atcattgtag ttataataaa ttaagtaagg gtcttttttc acaagcttta
    10741 aaaattcatc cccattgatt aagtaatgaa ttttaaagtt gtaatctaac tgattttgtt
    10801 ttattctacc catatcttga atctccaaag gataggttgc ttcttgatgg aatgcatagc
    10861 cttattttta cattctaaca caaagtgttg tcgtgtgtag tgttgattta ctttgtgcta
    10921 gaaaggtggt attatataag acacacatcg aggattaggt catggatata gatgccgaag
    10981 agattaaaat cctgaaagaa gagattagga acctaactca agaaatacaa accctaaaag
    11041 aagatctgca agctattaag gaaaccctgt atc
//
LOCUS       DATDRL010000083         5578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1111106, whole genome shotgun sequence.
ACCESSION   DATDRL010000083 DATDRL010000000
VERSION     DATDRL010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111106"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VI935_02925"
     CDS             complement(<1..1020)
                     /locus_tag="VI935_02925"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HLE24586.1"
                     /translation="MVSKIIRPKTIGIIGGGQLARMTAYAAYRMGLEIAILDPEPDSP
                     AGQIAHLKLVGSLNDVSKLEELARVSDLITLENEFVDAPLLKHLESIGECVFPTAYTV
                     GLIQDKLLQKEVLEKNGIGVPHFMGVSSYEDIYRAGEQFGWPLLLKTRRNGYDGRGNV
                     LIREPNDMLSAWKTLEGKSALMVESFVPFIKELAVMVARSRNGEILTYPVVETVQKDH
                     ICHIVKVPAGVDEDVSKMVATIARQAIEAVDGIGIFGIEIFFLEDKSVLINELAPRPH
                     NSGHYTIEACITSQYENHLRAILGLPLGSPAMILTAAVMVNLLGVRDAVANVSGLEKA
                     LSIPGV"
     gene            complement(1174..2154)
                     /locus_tag="VI935_02930"
     CDS             complement(1174..2154)
                     /locus_tag="VI935_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HLE24587.1"
                     /translation="MKKLLSLLLSLIILAVIYWKIDFHGLIMVIRNCNGWLMTISLGM
                     VVPLTLLTAIRLQQLMPHGARLGFGEANRLILVASVMNIILPMKMGDIAKAYFLKNQA
                     HLDGSYSLSLVIFEKACDMISLLLWCAFGLILYPKKDLFFWVMTGSVILGLITCLLLF
                     SSRKFTQICFALMELLVPKRFRSKLEKLHSSWDEMHDFFWSDKIQLFKIAGTSVFIWF
                     LHLLQIWFFILTLKAWTPFLANLALAPLAILVGLLPLTFAGIGTRDAALIVLYHPFFN
                     APTAAALGLLCTLRYLLPAIFGLPFLGKYLPTIRTMIKKPGTLSQVGTEL"
     gene            complement(2156..3745)
                     /locus_tag="VI935_02935"
     CDS             complement(2156..3745)
                     /locus_tag="VI935_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015145976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24588.1"
                     /translation="MSKEFFAKEAISQIPKILTLLDRNPHSPTYGCFDRNFWHYKIID
                     FPSGMAQEFVWPLALAYESNVTNNPFYKKPAIKEWVEAGILFASRSAHTDGSCDDYFP
                     YERALGATAFSLLACIESYILLGIKDETALRFFEKRGDWLANYQESGQLSNHQALITL
                     CLELLSRLFNTTKWDRLKEKRLERLLSWQSPEGWFQEYEGCDPGYLTLTISLLARLYE
                     LRPTPKLKESLVRAVNFAWHFLHPDGSYGGEYGSRNTYNFFPNGFELVGKWMPEALSI
                     NDHFLIGLANGLSPCYGDDHIIGLHAWSYLLAFRDFVVERSQPIPRPKGRTWFKEAKI
                     LIDRRDDTELYIALNKGGVFKLFRNNQLVLSDTQFSLMVKDGEIKNAVGHLIDKYKIN
                     INENEILIEGTLGWAKHIKTTPTALIAFRLLMISIGRLFPNLIRRLLQKMLITSKREA
                     PFTFSRRFRWENKRWKIIDELRAESWKNVVSAGIGCDQTSIYNVMSRTFQIGQLQPWY
                     DHTDEVKKLSPGQPLKLERNL"
     gene            complement(3818..4774)
                     /locus_tag="VI935_02940"
     CDS             complement(3818..4774)
                     /locus_tag="VI935_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLE24589.1"
                     /translation="MPKLIIQIPCFNEEETLGITLSTLPRSLPGIDSVEWLIINDGST
                     DRTVEVAKAHGVDHIVNFSHNRGLAKAFMAGLEACLKAGADVIVNTDADNQYYADDIP
                     KLIEPILLGKAEIVIGARPINQIKHFSLTKKFLQKLGSWVVRIFSNTDIQDVTSGFRA
                     ISRDAAMQLNVFNEYTYTLEMIIQAGQKGIAVTSIPIRTNQDLRSSRLIKSIPSYIQQ
                     SIFTILRIFMAYRPLRFFMIMGGIPFALGFLIGVRWLVLFLSGNPRTHLPSLVLASIL
                     ILIGFQLWVFGLIADLLAVNRKMLEDIQLQTRRSEFDSQKTK"
     gene            complement(4860..5477)
                     /locus_tag="VI935_02945"
     CDS             complement(4860..5477)
                     /locus_tag="VI935_02945"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLE24590.1"
                     /translation="MNIADYIISKHEKYTTRNPISRYLVGNFFTKIRGLLSDISFESI
                     LDIGCGEGILLHNIQGHLVGKNVFAVDIDHLEIETARKNIPSAKLGVASAHDLPFSDA
                     GFDLVICCEVLEHLDNPDLALREIRRVTAKYCILSVPNEPIWRILNICRGAYISDLGN
                     TPGHINHWSRRSFKQYVSDYFQIVETTTPLPWIGVLGHQKTNWKA"
BASE COUNT         1700 a         1141 c         1102 g         1635 t
ORIGIN      
        1 tactccaggg atacttaatg ctttctctag cccggagaca ttagcaacag catcacgcac
       61 ccctaacaga tttaccatta cagccgcagt taatatcata gcaggagaac ctagcggaag
      121 ccccagaatt gctcttagat gattttcata ttgagaggtg atacaggctt caatggtgta
      181 gtgtcccgag ttatggggac gaggtgcaag ctcgttaata agaacgcttt tgtcttcgag
      241 aaaaaaaatc tcaataccaa atatcccaat gccatcgaca gcttcaatag cttgcctggc
      301 aatagttgca accattttag agacatcttc gtccacgcct gctggaacct ttactatatg
      361 acaaatgtga tccttctgga cggtttctac tactgggtat gttagaatct ctccatttct
      421 acttcgtgca accattactg ctagctcttt gataaacggc acaaatgact caaccatcaa
      481 ggcagatttt ccttcgaggg tcttccatgc tgataacata tcgtttggtt ctcggattaa
      541 tacattgcct cgaccatcgt aaccattacg cctagtttta agtaaaagcg gccagccgaa
      601 ttgttcgcct gccctataaa tgtcttcgta gcttgaaact cccatgaaat gaggaacacc
      661 aattccattt ttctccagga cttctttttg taagagttta tcttgtatta gaccaacggt
      721 gtacgctgtc gggaacacac attcccctat agactcaaga tgtttaagta gaggtgcgtc
      781 aacaaactcg ttttcaaggg tgattaagtc ggacacacgt gctagttctt ccagcttaga
      841 aacatcattc aaagaaccga ccagctttag atgtgcgatt tgtccagccg ggctatctgg
      901 ctccgggtca aggattgcta tttctaaacc cattctataa gcagcgtaag ctgtcatacg
      961 ggctagttgt ccgccgccta taatacctat tgttttaggt ctaatgattt tactaaccat
     1021 tatgactgat gtcgttataa cctatacaat caagaaacaa ttttctacct acatacaata
     1081 ataaatttgg aaccgccatc tcttccatgc ttgttagagt ttaataaata acaattagtg
     1141 aaattcgtga tgatcttcaa attgaataat cttttacaac tcagtaccta cttggctaag
     1201 tgtaccaggc ttttttatca tagtgcggat tgttggtaaa tattttccaa gaaaaggtag
     1261 accgaaaatt gctggtagta aatagcgtaa tgtgcagagc agtcccaagg cagcagcagt
     1321 gggtgcattg aaaaaagggt ggtacaacac tataagtgca gcgtcacgtg tgcctatacc
     1381 agcaaatgta agtggaagca aacctactaa aattgcaagt ggagccagtg ccagatttgc
     1441 caagaaaggt gtccatgctt tgagtgtaag tataaaaaac caaatttgaa gaagatgcag
     1501 aaaccaaata aagacggagg tgccagcaat tttaaagagt tggattttgt cactccagaa
     1561 aaaatcatgc atttcgtccc aggaactatg caatttttct agctttgatc tgaatctttt
     1621 aggaacaagc aattccatca gtgcgaaaca tatctgtgta aattttctgg aactgaagag
     1681 gagaagacaa gtgataagtc ctaaaattac gcttcctgtc attacccaaa agaacaaatc
     1741 cttttttggg taaagtatta acccaaatgc acaccataag agtagagaaa tcatgtcgca
     1801 tgctttttca aatataacga gtgacaggga gtacgagcca tccaagtgag cctgattctt
     1861 aaggaagtag gcttttgcga tatcgcccat cttcatcggc aatataatat tcattacact
     1921 ggccacaagg attagacggt tagcttcacc aaaaccaagt cttgcaccgt gaggcatcag
     1981 ttgttgaagt cggatagcag tgagaagtgt aagaggcacc accatcccta agctgattgt
     2041 catcaaccac ccattacaat tcctgattac cattatgagt ccatgaaagt caattttcca
     2101 atagattact gctaagataa ttaggcttaa aagtaaggag agtagtttct tcatctcaaa
     2161 ggtttcgttc taatttcaat ggttggccag gtgagagttt tttgacttca tcagtatgat
     2221 cataccaggg ctgtaactgc ccaatttgaa atgttctact cataacgtta tagattgaag
     2281 tctggtcaca gccaataccc gcagagacga cattcttcca agactctgct ctaagttcat
     2341 caataatttt ccatcttttg ttttcccacc taaatcgacg cgaaaaggta aaaggcgctt
     2401 cccttttact agtgatcagc attttttgaa gtaatcttcg gatgagattt ggaaataaac
     2461 gacctatgct tatcattaaa aggcgaaatg ctataagggc tgttggagtt gtctttatat
     2521 gtttagccca tccaagagta ccctcgatta gaatctcgtt ttcattaata tttattttat
     2581 atttatcaat aagatggcca actgcgtttt ttatttcacc atcttttacc atgagtgaga
     2641 actgtgtgtc ggataacaca agttggttat tacgaaaaag cttgaataca ccacctttgt
     2701 tgagggcaat atatagttcg gtatcatccc tccgatcaat gagaatttta gcttctttaa
     2761 accaggtgcg accctttgga cgagggattg gttgtgatct ttctactaca aaatcgcgga
     2821 aggctaatag atagctccaa gcgtgaagac cgattatgtg gtcgtcacca taacagggtg
     2881 aaaggccatt agcaagccca ataagaaagt ggtcattaat actaagtgct tcgggcatcc
     2941 atttgcctac aagctcaaaa ccattaggga agaagttgta ggtatttctg ctcccatatt
     3001 ctcctccgta tgaaccatcg ggatgtagaa agtgccaagc aaaattgaca gctctaacaa
     3061 gcgattcctt tagttttggt gttggcctta gttcatacag ccttgctaag agtgaaattg
     3121 ttagtgtaag atagccagga tcacaaccct catattcctg aaaccacccc tctgggcttt
     3181 gccaagataa tagacgttcc agacgcttct ctttaagcct atcccatttt gttgtgttaa
     3241 agagccgaga cagtaattca agacaaagtg ttataagagc ttgatgatta gagagttgtc
     3301 cgctttcctg atagtttgca agccagtccc cgcgcttttc gaaaaaacga agcgcagttt
     3361 catctttaat acctaaaagt atgtagcttt ctatgcacgc taagagcgaa aacgctgttg
     3421 cacccaatgc acgctcgtaa gggaaataat cgtcacaaga accatccgta tgggcgcttc
     3481 gtgatgcgaa tagaattccg gcctcaaccc attctttaat agcgggcttc ttatagaagg
     3541 gattatttgt tacgttcgat tcgtatgcaa gcgctagggg ccagacaaat tcttgcgcca
     3601 taccgcttgg aaaatcgatg attttataat gccagaaatt gcgatcaaaa caaccgtaag
     3661 ttgggctatg aggattgcgg tccaggaggg tcaagatttt aggaatttgc gagattgcct
     3721 cttttgcaaa aaattcttta ctcatttaaa attaaattta aaccttaatg aaaagataat
     3781 taaaataatt ctgaacgatc aatccagttt atgaaagtta tttagttttt tgtgaatcaa
     3841 attcagaacg gcgagtttgt agttgaatgt cctcgagcat ctttcgattc acagccaaca
     3901 agtctgctat caagccaaat acccacaatt ggaatccgat aagaatgaga atagacgcta
     3961 agactaagct aggcaaatgt gtcctcgggt tgccgcttaa gaataataca agccagcgta
     4021 caccaatcaa aaatcctaac gcaaagggta taccacccat tatcatgaag aacctgagag
     4081 gtctataagc catgaaaatg cgcaagattg tgaaaattga ttgctgtata taggatggaa
     4141 tgctcttgat caagcgtgag gatcgtaaat cttggttcgt tcttataggt atggaggtaa
     4201 ctgcaatacc tttctgtcca gcctgtataa tcatttccag tgtgtatgta tattcgttaa
     4261 atacattgag ttgcatagcg gcatcacgac tgattgctcg aaaaccgctt gttacatcct
     4321 gaatatctgt attgctgaat atgcgtacaa cccaactgcc caatttttgg agaaactttt
     4381 tggtaagaga aaagtgttta atttggttga tagggcgcgc accaattact atttctgctt
     4441 ttcctaatag gattggttca ataagttttg gtatgtcatc agcatagtat tgattatctg
     4501 catcggtatt aacgattaca tccgccccag ccttaaggca agcttctaac ccagccataa
     4561 aggccttagc gaggccccga ttatggctaa aattgactat atgatcaaca ccatgtgcct
     4621 tagcaacctc aactgttctg tcagtacttc catcattaat aataagccac tcaacactgt
     4681 caatgccagg aaggctccga ggaagtgtgg aaagtgtaat cccaagagtt tcttcttcat
     4741 taaaacaagg gatttgaata ataagttttg gcatggattg aagcagaaga attgaatctt
     4801 gctacaaaaa tttacactaa gtggaaacta ataagccatc tgatattgca agtcatagcc
     4861 taagctttcc aattagtctt ctgatggccg agcaccccta tccatggaag aggggtagtt
     4921 gtctccacta tttgaaagta atcactgaca tattgcttaa atgacctcct actccagtga
     4981 tttatatgac caggtgtgtt tcccaaatca gaaatatatg caccacggca aatgttgaga
     5041 atacgccaga tcggttcgtt tggtacagat aaaatgcaat actttgcagt cacacgccga
     5101 atctctcgca atgcaagatc aggattatca agatgttcta aaacctcaca gcatatcact
     5161 aagtcgaatc ctgcgtcaga aaaagggaga tcatgggcac ttgcaacccc aagtttagct
     5221 gaaggaatat tcttccgcgc ggtctcaatt tctaagtgat ctatatcaac agcaaagaca
     5281 tttttcccaa ctaaatggcc ttgaatattg tgtagaagaa tcccttcacc gcaaccaata
     5341 tctagtatgc tttcaaatga gatgtcggaa agcagccccc tgatcttggt gaagaaattc
     5401 cctactaaat atctgcttat cgggttgcgt gttgtatatt tttcatgctt gctaattatg
     5461 tagtctgcta tattcattct ttatgatata tcgcgtgtgg atattttcaa atgtagaaaa
     5521 atactaaaga taatttagat ataaaagagg ttaaagtctc aaccaattta aatagcta
//
LOCUS       DATDRL010000084         4471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2417869, whole genome shotgun sequence.
ACCESSION   DATDRL010000084 DATDRL010000000
VERSION     DATDRL010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2417869"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..101
                     /locus_tag="VI935_02950"
     CDS             <1..101
                     /locus_tag="VI935_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HLE24591.1"
                     /translation="TISTHRARILGKMKMKTNAELTHYAIKNRLVD"
     gene            623..2653
                     /locus_tag="VI935_02955"
     CDS             623..2653
                     /locus_tag="VI935_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HLE24592.1"
                     /translation="MKKLLSALIVLGMLFAFSFFIGCDNDGVDGGGGGPPIDSNDIGG
                     TVTSNPPEAGVWVIAETDNLPTKFRKIVVTDDNGNFVIPDLPPATYNVWVRGYGLKDS
                     TPVQASPGEIIELQAVQAATPQEAAQVYPANYWYSLLEAPSDERVAEEGFPNQASWVN
                     FIKLGCELCHQVGNEATRLPNGAAFDAGMRLAGTMNVYADILGRDLFIEVFGDWGERI
                     AAGEVPPEPPRPQGIERNLVITQWEWGDQYAYVHDEIATDKRNPFLYPNGKVYGVDIG
                     MDRLVAVDPVTNEAFEYDVPTRGGYDVPWCESTGKALDPGSSPFPVGFCTWVVYHNPA
                     NIHNPMFDDTGKVWVTTQNRREWTEDLPDFCLDDPVIANNYHHRQLGYFDTATEEFVL
                     VDTCYGTHHLQFDADDVLWTSGDSFVLGRFDTTLFDPNDPSTLEDAQSWFEVRVDSDG
                     DGVADTPTPGFNYGIIPNLVDGTIWTAVPGYPSTIRRFDPATEKFEIYAPPAPGHGAR
                     GIDTDTEGNIWTCLGGSGHVAKFDRSRCAQTWGTGDQCPEGWEMWEAPGPQMKGVSDG
                     PNEGSADFFYYGWVDQFNTLGLGENTVICNGTGSDSLMAFNPSTEEFTIIRMPYPLGF
                     FQRGLDGRIDDPNAGWKGRGLWVDYGGDPAKHVETRMGQVCKVQLRPDPLAH"
     gene            2850..3752
                     /locus_tag="VI935_02960"
     CDS             2850..3752
                     /locus_tag="VI935_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24593.1"
                     /translation="MNRLFFSTVNLVILFFACVSYAGDSDLVNPYIANYPFKSTVIHY
                     AIKDEYGHGKISKGTEVVYIKGDRLAKVTKMAVPDPKEMTKTKNVETLQIFTPDYIYM
                     VDLVEKTGSKIDNSKKYTKPAYDKLSDEEKKAFHRRMERRGIVSLDLLGLGKKVGTDT
                     VLGRQCDVYQSGKEMSPEELVAASESGESPSYMKSWIWGTAKIPLKVITAGLGWSNEL
                     IATKIEESVKIPDSQFTAPSDIKVTYDKEKSEFAKQEALARFELYKTGKPMVVKTKLK
                     REEIKPKGDSKASESNQKSQENQK"
     gene            3887..>4471
                     /locus_tag="VI935_02965"
     CDS             3887..>4471
                     /locus_tag="VI935_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLE24594.1"
                     /translation="MIKILIADDHAVVRRGLKQILAEEPNMAVFGEACNAQEVLKNVR
                     EQNWDIVILDITMPDRSGLEVLKELKNIRPKLPVLILSIHPEEQYAVRVLKAGAAGYM
                     TKESAPEELVKAVRKVIRGSKYISPSLVEKLAFDLETDSEKPLHETLSDREYQVMCMI
                     ASGKTVKEIAKKLYLSVKTISTHRARILGKMKMKT"
BASE COUNT         1362 a          871 c         1107 g         1131 t
ORIGIN      
        1 aaaccattag cacgcatcgc gcgcgcattc tgggaaaaat gaaaatgaag acaaacgcag
       61 aattaaccca ttatgccata aaaaaccggt tggtagattg aaacgaccat ttaattcctt
      121 tccacattgt aggacaaacg ctgacaacag agcaagtcaa agtcctacaa aaaaatcaga
      181 cttctgctga tagttttcca aagtcttata tgtcttaatc ttacagtgat acaaatgttt
      241 ttgtgcaaaa gcttgttagg cagagattca caagccttat aggaggatgg gataaagcta
      301 atggacatag gtgcggttct ttcttaccct gaaaagaaga agcattttaa aagcagaatt
      361 ataaaatgcg gggttaaaaa agataaacct attgtataag ccctctagga gggattatct
      421 ctagagggct atttccttta ataataggta tcaatacttc ggacagtttt tataatttac
      481 tgaaattaaa aggaatttca attcgttgtt cttaacctaa aaacccttta atttcagtga
      541 gttagatata ttatctgcag aaaatgtcca aactattgag gtatgaaaaa agaaaaaata
      601 tacacaaagg aggtaagaag tcatgaaaaa attactaagt gcgcttatag tgctaggcat
      661 gttgtttgcc ttttccttct tcatcggttg tgataatgat ggagtagatg gtggaggggg
      721 tggaccacca attgattcta atgatatagg tggtactgtt acgagtaatc ctcctgaagc
      781 tggggtgtgg gtaatagcag agacagataa ccttcccact aagtttcgta agatagtcgt
      841 tactgatgat aacgggaatt tcgtcattcc agacctgccg cctgcaacct ataatgtatg
      901 ggtgcgggga tacggtctta aggattcaac acctgtccaa gcctcaccgg gtgagataat
      961 tgaactccag gctgttcagg cggctacccc acaagaggca gcacaggtct atcccgcaaa
     1021 ttactggtat tcactccttg aagccccctc tgatgagagg gtcgctgaag agggttttcc
     1081 taatcaagca tcctgggtaa attttatcaa gctgggttgt gagctctgcc accaggtggg
     1141 caatgaggct acgcggctcc caaatggtgc agcgtttgat gctggcatga ggttggcggg
     1201 aacgatgaat gtgtacgctg atattcttgg cagagatctt tttatagagg tcttcggcga
     1261 ttggggagaa agaatagccg cgggtgaggt tcccccagag cctcctcgac cccaggggat
     1321 agaaagaaat ttagttatta cacagtggga atggggagat caatatgcgt atgtccatga
     1381 cgagatagcg acggataaac gtaacccctt cctgtatcca aatggaaagg tatatggtgt
     1441 tgatattggg atggaccgcc ttgtggcagt tgaccctgtt accaacgagg cctttgaata
     1501 tgatgtccct acacgtggcg gatatgacgt tccgtggtgt gaatcaaccg gtaaggcact
     1561 agatcccggc agcagtccat ttccggttgg cttctgtact tgggttgtgt accataaccc
     1621 tgctaacata cataatccta tgtttgatga tacgggcaag gtatgggtga caacacagaa
     1681 taggcgagaa tggaccgagg atctaccaga cttctgcctg gatgatcctg ttatcgccaa
     1741 caattaccat caccgtcagc tcggatactt tgacactgcg acagaagaat tcgtgctggt
     1801 tgatacctgt tatggaacac accatcttca gtttgatgca gatgatgtac tctggacgag
     1861 cggggacagt ttcgtccttg ggcggtttga caccaccctg ttcgacccga acgatcccag
     1921 tactctggag gatgcgcaaa gctggtttga ggtaagggtg gattcagatg gggacggcgt
     1981 agccgatacc cctacccctg gttttaatta tggaattatc cctaatttgg tggacgggac
     2041 aatctggaca gctgttcctg gttacccatc aacaattagg cgctttgacc ctgcgacaga
     2101 aaagtttgag atttatgcac ctccagcacc cggacatggt gcaagaggga ttgatacgga
     2161 tacagaagga aatatatgga cttgtttagg tggcagtgga catgtagcaa aatttgaccg
     2221 cagtagatgt gcgcagacgt ggggtacggg agaccagtgt cctgaaggat gggaaatgtg
     2281 ggaagccccg ggacctcaaa tgaagggtgt atcggatgga cctaatgagg gtagcgccga
     2341 tttcttctac tacggatggg tcgaccagtt taatacatta ggactggggg aaaatacggt
     2401 tatttgcaac ggtacgggtt cagattcact catggctttc aacccatcga ccgaagagtt
     2461 tacaataatc cgtatgccct acccgcttgg tttctttcaa cgtgggctgg acggcaggat
     2521 tgacgacccg aatgcaggct ggaagggtag aggactatgg gtagattacg gtggagaccc
     2581 tgccaagcat gttgaaacta ggatggggca ggtttgcaaa gttcagcttc gtccggatcc
     2641 gctcgcgcat taagagagag caagttttgg gggactcatt ttggagtcct cctgttagct
     2701 gactatagca tgagttttga caatttaatc gggtaatcgt cattagtcgt tggtcttttg
     2761 tctttagtca aacgacaaag agctttattg cccgtttaat aaatcaaacc ccattttaac
     2821 taaaaacagt agttcaattt ggaggaaaga tgaatagatt gtttttctct acagttaact
     2881 tagtaatact tttttttgcc tgtgtctcat atgccggtga ttcagacctt gtaaatccgt
     2941 atatagcaaa ctatccgttt aaaagcactg ttatccacta cgctatcaaa gacgagtatg
     3001 gtcacggtaa aatttctaaa ggtacagaag tagtctatat aaaaggcgat aggcttgcga
     3061 aagtaacaaa aatggctgta cccgacccca aggaaatgac caagactaag aatgtagaaa
     3121 ctctacagat ttttaccccc gattatattt atatggttga tttagttgaa aaaacagggt
     3181 caaagataga taactcgaaa aaatacacga agcctgctta tgacaagcta tcagatgaag
     3241 agaaaaaggc ttttcatagg agaatggaga gaagaggaat tgtctcttta gacctactcg
     3301 ggttaggaaa gaaagtaggc acggatactg tcttagggcg acaatgcgat gtttaccaat
     3361 ctggaaagga aatgagtcca gaggaattag tagcggcatc ggagagtggg gagagcccct
     3421 cttacatgaa gagttggatt tgggggactg ctaaaatccc ccttaaagtc ataacagcag
     3481 ggttaggttg gtctaatgaa ctaattgcaa caaagataga agaaagtgta aaaatccccg
     3541 atagccagtt tacagcgccc tctgatataa aggttactta cgataaagaa aagtcggaat
     3601 ttgcaaagca agaagcccta gcaagatttg agctttataa gacggggaag ccaatggtgg
     3661 ttaagacgaa gctgaagaga gaagagataa agcctaaagg agattccaag gcaagtgagt
     3721 cgaaccaaaa gagtcaggaa aatcaaaagt gaaataaaca aacctacaat gctccctgga
     3781 ccaaatacac taggatgtag atgatgatag gcggagcccc atcacctacc acctattacc
     3841 tatcacccat cacttaccac ctatcaccta acggaggagc aacgccatga taaaaatcct
     3901 catcgcagac gatcacgccg ttgttcggcg agggttgaaa caaattctag ccgaggagcc
     3961 taatatggca gtgttcggcg aagcgtgtaa tgcccaggag gtgctcaaaa atgtccgcga
     4021 acagaattgg gatattgtga tattggatat aaccatgcca gacagaagtg gattagaggt
     4081 gttaaaagaa ttaaaaaata tacgcccaaa actccctgtg cttatcttga gcatacaccc
     4141 cgaagagcaa tatgcagtac gggttttaaa agctggtgcg gctggctata tgaccaaaga
     4201 gagcgcaccg gaggaattag tcaaagctgt aagaaaagta attagaggca gcaaatatat
     4261 cagtccatct ctcgttgaga aattagcctt tgatttagaa actgactctg aaaaaccgct
     4321 tcatgagacc ctgtccgatc gtgagtacca ggtaatgtgt atgattgcct cagggaaaac
     4381 ggtaaaggag attgctaaga agttgtacct gagtgtaaaa accattagca cgcatcgcgc
     4441 gcgcattctg ggaaaaatga aaatgaagac a
//
LOCUS       DATDRL010000085         2475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_847242, whole genome shotgun sequence.
ACCESSION   DATDRL010000085 DATDRL010000000
VERSION     DATDRL010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847242"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VI935_02970"
     CDS             <1..194
                     /locus_tag="VI935_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24595.1"
                     /translation="IDASHDYSALLVGHVKEEHTKRNHLPITYWGIYLDDKYVSYTSS
                     KERAEKTKIWIEKWLKDRL"
     gene            341..1444
                     /locus_tag="VI935_02975"
     CDS             341..1444
                     /locus_tag="VI935_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLE24596.1"
                     /translation="MDWGLHFPSHIHSWKWVSYGEQKGFSYFMPYDSIGSDVYETLAL
                     CAINTKTIKLGPGVTSPRSRIAPVTARAMATLNELAPGRCVLGIGTGNTARRAWGMPA
                     AKIEEVREDVEICRKLFRGEEALYVEGPDRYTREERRRYVKFLSPDWGFINIKDSIPI
                     FLAGSGPRMLELAGEIGDGVILLGAIGKSFVDYCMGYIKVGAKKAGRDPKSLYTTVLT
                     AFHILQKGETLDSKSVKVALGPVVAVCLGIAALSIVNYETGKVGTAKGEPLPEDIREG
                     IMSFTDTYAIDESIERKHLKRYSNYFGWKEEYDPLFTPEMIKASTLVGTPEEIRETIH
                     RLESQGINQVMIAPIPDPNQAIDSFHENIMSKH"
     gene            1909..>2475
                     /locus_tag="VI935_02980"
     CDS             1909..>2475
                     /locus_tag="VI935_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010479220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLE24597.1"
                     /translation="MRCSSCGFENPEEMIFCGKCASPVSLRCPQCGYENPPEFAFCGK
                     CAATLTGQSPVTKSTQRDIQLDKQEDKAAPEPERRQLTVMFCDLVGSTSISESLDPEE
                     LRDVVRTYQETCSELIDRYDGYIAQYLGDGLLVYFGYPLAHEDDAQRAVRGGLEIVGA
                     ISELSLPTLLQESLQVRVGIHTGLVVVGE"
BASE COUNT          728 a          465 c          633 g          649 t
ORIGIN      
        1 caattgatgc aagccatgac tactcagcgt tgctggttgg acatgtaaag gaagaacaca
       61 ccaagagaaa tcatctccca attacttact ggggaatcta tctcgatgac aaatacgtat
      121 cctacacatc cagtaaagag cgggctgaaa agacgaagat atggatagag aagtggctga
      181 aggacagact ttaaaattta aaatagagaa atgcgtgaga aaattcctat tgcaatgggt
      241 tgttaatggt gagacaatag ctatagcaaa ggtttatgca taaaaaagtt aattttattc
      301 taaaaagaag gtaaaaatct caaaaaagga ggtaaaagct atggattggg gattacattt
      361 tccgtcacat atacattctt ggaagtgggt atcatatggg gaacaaaaag ggttttcgta
      421 ctttatgccc tatgatagca ttggcagtga tgtctatgaa accctggcac tttgtgccat
      481 aaatacaaaa accataaagc taggaccggg tgttacaagc cctcggtcac ggattgcccc
      541 ggttaccgcc cgtgctatgg caactcttaa tgagttggcc ccgggaagat gcgtcctggg
      601 tattggcaca ggaaacaccg ctcgtcgcgc ctggggaatg cctgctgcaa aaatcgagga
      661 ggtaagggaa gatgtcgaga tatgcaggaa gttgtttagg ggtgaagaag ctctttatgt
      721 tgaaggtccg gatagataca cgagggaaga gaggcgccgc tacgtgaagt ttttaagccc
      781 cgactgggga tttatcaata ttaaggactc tattcctatt tttttagcgg gaagcggccc
      841 caggatgctg gagttagcag gtgagatagg agacggcgtt attttactcg gcgctatagg
      901 taaatctttt gttgattact gcatggggta tatcaaggta ggggcaaaaa aagcgggaag
      961 agacccaaag agcctgtata caaccgtgct cacggctttt catattcttc agaaggggga
     1021 aacattagat tcaaaatcag taaaggttgc gttaggacct gttgtagctg tctgcctggg
     1081 cattgcggct ctttcgatag tgaactatga aacgggaaag gtgggtacgg ctaagggtga
     1141 gcctctgccg gaggatatac gtgaaggtat tatgagcttt acagacacat acgcaataga
     1201 cgagagcatt gaaagaaaac acctaaagcg ctacagcaat tatttcgggt ggaaggaaga
     1261 gtacgatcct ctgtttacac cggaaatgat aaaggcctct acgctggttg gtactcctga
     1321 ggaaatccga gagactattc acaggctgga gtctcagggc ataaatcagg ttatgatagc
     1381 tcctatacca gaccctaacc aagcaataga tagcttccat gaaaacatta tgtcaaagca
     1441 ctagagacgc gagtagctag tgcaaggtta aataattgtt tttcatttag aacttaagta
     1501 agaatcgccg ttcgtggttc gacacttcga caggctcaga gctcaccatg aacggctgaa
     1561 ttcaaatacc gttcgccctg agcttgttgt cgaagagcgt gtttttttgc ccaagtttga
     1621 tcaaaactaa gttagcaata tacttctgca tttcctgata taaggaaaag attttctctt
     1681 ataccatttc caaaaagaaa atggctgttg taggcacgaa cttgttcgtg caaattctta
     1741 cggctatggc acaggcgagt ctgtccctac aatatttgaa cacttatcaa cttctgaatt
     1801 ttagaaagga gaggtaagat ggaaacctgg atcataggat caactagagc atagcttttt
     1861 gtagttttta gctctattgt tgtaaactaa gtttaggaga tcaagggaat gcgctgctcg
     1921 agttgtggat ttgaaaatcc ggaagaaatg attttttgtg gcaagtgtgc cagcccggtc
     1981 agtcttcgct gtccgcagtg tggctatgag aatccacctg agtttgcttt ctgtggaaag
     2041 tgcgctgcca ctctcacggg gcaaagtcct gttaccaaat ccacccagag agatatacag
     2101 ttagataaac aggaagataa agctgcgcct gagcccgagc gtcgccagtt aacggtgatg
     2161 ttttgcgatt tggtgggttc tacaagtatc tccgaaagtt tagaccctga agaacttcgc
     2221 gatgtggttc gtacctatca agaaacctgt tcagaactga tagatcgcta tgatggatat
     2281 attgcgcaat atctgggtga cggattactg gtctactttg gttatccctt ggcacacgaa
     2341 gacgatgcac aaagggcagt aagaggaggg cttgaaattg taggggcgat tagcgaattg
     2401 tccctaccca ctttgttgca agaatccctg caggtgcgtg tagggattca cacaggtctt
     2461 gtagtggttg gagag
//
LOCUS       DATDRL010000086         2415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_46740, whole genome shotgun sequence.
ACCESSION   DATDRL010000086 DATDRL010000000
VERSION     DATDRL010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46740"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VI935_02985"
     CDS             <1..460
                     /locus_tag="VI935_02985"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HLE24598.1"
                     /translation="VSIPLLRKDFIIDPYQIYEARYYGADAVLLIAAALDTRTITELL
                     SLTRSLGMNAIVEVHDEEELERALLAESKIIGINNRDLKKFTVCLDVSIRLSRLIPAG
                     KIVVSESGLTSSEEIKRLKNEGIRVFLIGETFMKATNPGEELRAMLAECD"
     gene            1114..1356
                     /locus_tag="VI935_02990"
     CDS             1114..1356
                     /locus_tag="VI935_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24599.1"
                     /translation="MADYGFLYVIDTSYHTERQVYQINIGYMDRDEEVKGRFITINIV
                     VNIPNHKIGENKIKELALAKARKLLELASKAPEGEK"
     gene            complement(1481..>2415)
                     /locus_tag="VI935_02995"
     CDS             complement(1481..>2415)
                     /locus_tag="VI935_02995"
                     /inference="COORDINATES: protein motif:HMM:NF020022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLE24600.1"
                     /translation="GDLHLEHINLSPQGVCIYDCIEFNERFRYIDIASDIAFVAMDLD
                     FNDYSDLANFIVQEISKRMKDEKIFEVIDFYKCYRAYVRGKVESIRSTETEIPSEERE
                     ASQEKATRYFRLALRYALFGSRPALVVTFGLIGTGKSTLARSLSEELSCKVISSDEVR
                     KEIMGVKPTARRYEEFDKGIYSPNITNVTYRELLNRGRRVIESGKTVILDASFSKREF
                     REVVLHEARALGVIFYFVETKASEEAIRKRLIDRERGEKSISDARWEIFEKFKEGFEE
                     TNELPGDKHIIVRTDKTPEETLIQTMREIICRGQ"
BASE COUNT          751 a          440 c          440 g          784 t
ORIGIN      
        1 ggtttcgatt ccacttttaa gaaaggattt tattatagac ccgtatcaga tttacgaggc
       61 tcggtattat ggagcggatg cagttctctt aattgctgct gcgcttgata caaggacaat
      121 aactgagctc ttgagtctaa cacgctcctt aggtatgaat gccattgtag aggtgcatga
      181 tgaagaagag ttggaaagag cacttctagc tgaaagcaaa atcatcggga taaataacag
      241 ggacttaaaa aaatttactg tttgcctcga tgtttcaata agactttcaa ggttgatacc
      301 agccggcaaa atagtggtga gcgaaagcgg gcttacatca agcgaagaaa ttaagagact
      361 aaaaaatgaa gggattcgtg tctttcttat aggtgagaca tttatgaaag caactaaccc
      421 aggagaagaa ctcagggcca tgctagctga gtgcgattag caaattgatt gtggattaat
      481 tcctctccga atgttcagaa aaagttaagg aactccaaac attttttatt gttgccttac
      541 ctcttttgcc attttaagta aataattatg caacgatgaa ctattctttt tcagacagca
      601 aatgcgggct aaagccatcc gctacttgta gggaagagta gtgaatccct caggattcca
      661 aggtttgcct ttattcatag atataacatg agcacaagaa atctagttaa gatttttaac
      721 ccaaaatacg ttgacttgac aggtgcaagc gatacaaatg gaaagcctgg gtatacactc
      781 tttatttcgt aatttaaatc agggtggatt taagagcctg attcactaag tagcaagatt
      841 gttagaatgg gataaatata tcacggcttg gcttatgcaa cgtaacctta tatttttttc
      901 ggtaattata cgcagctttc ttcaataagt taatttaagt ttatgtaaga tgtcagaaaa
      961 taggacatta atccgtgacg ttcagcctta attatattgt aattttagaa aatagttatt
     1021 ggcacgtatt ttgattgata aatttgtaga agattgcaat acgaaacggt ttatgttaaa
     1081 agaatgaatc aataaatagt ttgagagggt aaaatggctg attatggttt tctttatgtt
     1141 attgatacct cttatcatac tgaaaggcag gtataccaaa taaacattgg atatatggac
     1201 agggatgaag aagtaaaagg tcgtttcata acaattaata tcgtagtaaa cattccaaat
     1261 cacaagatag gtgaaaataa aattaaagaa ctagctcttg ctaaggcaag aaaattgcta
     1321 gagttggctt ccaaagcacc tgaaggagaa aagtaaagtc accctacaga tacgtttgag
     1381 tagaataaaa ttgtaaatgg agtgaacaca cacttatctt ctatagtaaa ggaggttgat
     1441 ttcatccttg tattggttta tcaagagggc taatagataa ttattgtcct ctgcatatta
     1501 tttctctcat agtttgtatt aacgtctctt caggtgtttt atcggtcctt acaataatat
     1561 gcttgtctcc aggaagctca ttcgtttctt caaatccctc tttaaatttt tcaaatatct
     1621 cccacctcgc atcagagata gatttctctc ctctttccct atctataagt cttttcctta
     1681 tggcttcttc acttgctttt gtttcgacga agtaaaatat taccccaagt gcccgggcct
     1741 catgaagaac aacttccctg aattcccttt tcgaaaagct tgcatcgagt ataacggttt
     1801 tccctgattc gataaccctt cttcctctat ttaataactc cctatatgtt acattggtaa
     1861 tatttggtga ataaatcccc ttatcaaatt cctcatatct tcttgcagtt ggtttcacgc
     1921 ccatgatttc ttttctcact tcgtctgaag aaataacctt acaagaaagc tcttctgata
     1981 gcgaacgggc gagtgtgctc tttccggttc ctataagccc aaaggttaca actagggcag
     2041 gtcttgaacc aaaaagtgca tatcgtagag cgagtctaaa ataccttgtt gctttttctt
     2101 gagaagcctc tctttcttca ctaggaattt ctgtttcagt gcttcttata ctttctactt
     2161 tacctcttac atacgcccta tagcatttgt aaaaatcaat cacctcgaaa atcttttcat
     2221 ccttcatcct ctttgaaatt tcttgtacaa taaaatttgc aaggtcacta taatcgttaa
     2281 aatctaaatc cattgctaca aaagcgatat ctgaagcgat atctatgtat ctaaatctct
     2341 cattaaattc tatgcagtca tagatgcaaa cgccttgagg gcttaggttt atatgctcaa
     2401 gatgaaggtc gccat
//
LOCUS       DATDRL010000087         3084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3002736, whole genome shotgun sequence.
ACCESSION   DATDRL010000087 DATDRL010000000
VERSION     DATDRL010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3002736"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VI935_03000"
     CDS             complement(<1..287)
                     /locus_tag="VI935_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HLE24601.1"
                     /translation="MYGRIRNIHFVGIGGIGMSGIAEVLLNMGYGVTGSDLKRTDIID
                     RLEQLGARIFINHSAENIGNADVVVTSSAIKKNNPEVLAAQSQKIPVIPRA"
     gene            complement(364..1473)
                     /gene="murG"
                     /locus_tag="VI935_03005"
                     /pseudo
     CDS             complement(364..1473)
                     /gene="murG"
                     /locus_tag="VI935_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
     gene            complement(1470..2582)
                     /gene="ftsW"
                     /locus_tag="VI935_03010"
     CDS             complement(1470..2582)
                     /gene="ftsW"
                     /locus_tag="VI935_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HLE24602.1"
                     /translation="MFKIFESKGTGYDWGIIASVIFLSSMGVLMVYSTSSVFALEKFG
                     DSSYFLKRHAIYLSLGFAVMIILMKLNYSVLRGLVYPAYVIGLVILALVLIPGVSKEI
                     GGARRWINLGIWGFQPAEIAKFLLIFYLSYSLMKKKEKMDTFLIGFFSHLVIAGSYLI
                     LILLEPDFGMSAILLTVLFGMLFIGGVKMRYLAFSSFVAVIFLVWAVMSEGYRMSRVT
                     TFLNPWKDPLGSGYQAVQSFTAFGLGGIQGTGLGDSTQKLLFLPEAHTDFIFSIIGEE
                     LGFIGIVLIIFAFGFLMIRGIRVALRAPDLFGCYLVFGFIMLVTLQAGVNMAVAVGLF
                     PTKGLTLPFISYGGTSLVSSLASMGIVLSVSRYGIK"
     gene            complement(2566..>3084)
                     /locus_tag="VI935_03015"
     CDS             complement(2566..>3084)
                     /locus_tag="VI935_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HLE24603.1"
                     /translation="GKHNIENAMAAIASTRILECDPRLIEETIINFNPLPHRIEFVRE
                     LKGVKFYNDSKSTSPGATLRALECFTSPIILIAGGKDKGGGYEVLKDTLRERVKLLVL
                     FGESKFRIEKYLGKEVETVLAFSLEEAIEKAFKDSEKEDTVLFSPACSSFDMFSSYEE
                     RGRRFREIVQNL"
BASE COUNT          921 a          734 c          542 g          887 t
ORIGIN      
        1 tccgccctgg gaattacagg aatcttttga gattgcgctg ctagaacctc aggattattt
       61 ttctttatag cagaggatgt aacaactaca tcggcgtttc caatgttttc tgcgctgtga
      121 tttatgaaga tccgtgcccc aagttgttcg agcctatcaa taatatctgt tctttttaaa
      181 tcagagcctg taacgccata gcccatattg agaagcactt cggctatacc gctcattcct
      241 attccgccaa tgccgacaaa gtggatattc ctaatccgtc cgtacatatg aatatgctcc
      301 aacgaccaaa ttacaaactc caaacattag ccactcctgc atagagaatt cagaacccat
      361 gagtcagtag tcggaagagt tcttctgcat tctgactcct ggattctgaa ttcttgcaag
      421 tttatacatc tcgtcaacta ttctttgtgc tgcatcgggt ttgccgagaa cccttgctcg
      481 ctcgctcatc tctttaagtt tatccttgtt aagtaagtcc tttaaaatat cggtaagccg
      541 ttcaggaaca gcatcttttt cttcaactac aactgcagcc cctgcctttt ctaaaacctt
      601 tgcgttttct aactgatgat tgtaagcaga aaatgggtag ggaacaagta ttgatggttt
      661 tcctagcgct gtaatctcag caatggttcc tgctccagcc cttccaatca ctaaattagc
      721 acttatgtat acatttgcta tatcttctat gaaagggaga acatccgctc gaatcccgta
      781 ccattcatag gccttcctta cgtcctcaag atcttcttca cctgtttgat gaattaaaaa
      841 gacgtctttc ctacggaggg ttccaaaagc ctttggaact aggaggttaa gcttatgtgc
      901 gccttggctt cctccaaaaa caaggatagt gaaaccattg tctgcctggc tatactcctt
      961 atttatcaga atgctgcttc ttacgggatt tccaactact atagtttttt tttctgaaaa
     1021 aaacctattg ctttcttcga aagaggcaaa aacccttttt acaaatctac taaggaccct
     1081 atttgtaata ccgggaaccg agttctgctc acatatagct gttggaattg aaagcagagc
     1141 tgctgcaaaa actagcggac ctgaagcata accaccaaca ccaagaacta aatctggctt
     1201 gaacctactt atgattctca aagaatcaaa aataccctat aaagccaaag ccataccttt
     1261 aagacttcct acgacgcctt ttccctttat acccccagaa ctgataagtt caattgtgta
     1321 ttctcttttt gaaaggagcc ttatttccaa cccccttttt gtgcctacaa aaagaacctg
     1381 gttttcaata tgccttttta agatttcctc tgcaattgag atggctggaa atatgtgtcc
     1441 ccctgtgcct cctcctgcga ttataacctt cattttatcc catatcgtga aacactaagc
     1501 actataccca tggatgcaag gctgcttact agagacgttc cgccatagct gataaaagga
     1561 agtgtcagcc cctttgtggg aaataagcca actgcaacag ccatgttaac ccctgcctgt
     1621 aaggttacaa gcataatgaa accaaaaaca aggtaacagc caaaaagatc aggcgctcta
     1681 agcgctaccc taattcctct tatcatgaga aagccaaacg cgaagattat taatacgatg
     1741 ccgataaatc caagttcctc tccaattatt gagaatataa aatcggtgtg tgcctctgga
     1801 agaaatagga gcttctgtgt actatcaccg aggccggtcc cttggatacc gccgagtcca
     1861 aatgccgtaa aagactgaac agcttgataa cccgatccaa gcggatcctt ccaggggttt
     1921 aaaaatgttg tgacacggct cattctataa ccttcgctca tcactgccca tactagaaat
     1981 ataactgcaa caaaagatga aaaagctaaa tatctcattt ttacaccacc aataaaaagc
     2041 atcccgaaaa gcacagtaag cagtattgct gacatcccaa aatcaggctc aagaaggatg
     2101 agaattaaat aagaccctgc tattacaaga tgagagaaaa agccgatcag aaacgtatcc
     2161 attttttctt ttttcttcat tagggaatat gacaaataaa agatgagaag aaattttgca
     2221 atctctgcag gctgaaaacc ccaaattccg agatttatcc atcttcttgc cccgcctatc
     2281 tccttgctta ctccaggaat aagcactaaa gcaagtataa ctaagccaat tacataagct
     2341 ggataaacga gacctcttag aacgctgtag ttgagcttca tcaatataat cataactgcg
     2401 aagccaaggc taaggtatat agcgtggcgt ttaaggaagt agcttgaatc tccaaatttc
     2461 tccagagcga agacggaact tgtgctgtaa accataagca cacccatcga tgagaggaat
     2521 atcacacttg caatgattcc ccagtcatac cctgttcctt ttgactcaaa gattttgaac
     2581 aatttcccta aacctccttc ctctttcttc ataagagcta aacatatcga agctcgagca
     2641 ggcaggtgaa aagagaacag tatcttcctt ctcagaatcc ttgaaggctt tctctatcgc
     2701 ctcctcaagg gaaaaggcta aaactgtttc aacctctttt ccaagatact tctctattct
     2761 gaattttgat tcaccaaaaa gaacgagaag cttaaccctt tcccggagtg tatccttaag
     2821 gacttcataa cctccgcctt tatcctttcc tccagcaata agaataatgg gtgacgtgaa
     2881 acactcgaga gccctaaggg ttgccccagg acttgttgat ttagaatcgt tataaaattt
     2941 gactcctttt agctcacgaa caaactcaat tctatgtgga agcgggttga aatttattat
     3001 tgtctcctca atcaatctag gatcacactc taagattctt gtagaggcaa ttgcagccat
     3061 tgcgttttct atgttgtgct tacc
//
LOCUS       DATDRL010000088         5741 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2164039, whole genome shotgun sequence.
ACCESSION   DATDRL010000088 DATDRL010000000
VERSION     DATDRL010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164039"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VI935_03020"
     CDS             <1..850
                     /locus_tag="VI935_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HLE24604.1"
                     /translation="AVYGEFPDVQTIAEESTAWPMVSRPVYVGGLGFGMKWNMGWMHD
                     TLDYFSTDPIYRKFHHNQLTFSIWYAFSENFVLPLSHDEVVHGKGALIGKMHGDEWQR
                     NANVRALFGYMYGHPGKKLLFMGGEFGQWREWVHDESLEWHSLQYPFQRGVQLWVKDL
                     NHLYRTEHALYELDFSFEGFEWIDFRDSENSIISFIRKGKYTNDIILVVCNLTPAPRF
                     NYQLGVPRGGFWKEILNSDSVFYGGSGLGNSGGLEANYVSSHGRPFSLFLTLPPLSVL
                     FFKSEI"
     gene            1366..3792
                     /locus_tag="VI935_03025"
     CDS             1366..3792
                     /locus_tag="VI935_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HLE24605.1"
                     /translation="MDKYICIHGHFYQPPRENPWLEEIEYQDSAHPYHDWNEKITAEC
                     YAPNGASRILDHEGKIVEIVNNYSKISFNFGPTLLGWLEKKSPDVYQAILEADRVSQN
                     NFSGHGSALAQVYNHIIMPLANPRDKYTQALWGIRDFERRFGRKPEGMWLPETAVDLE
                     TLDILAGLGMRFAILAPHQASRVRRIGARTWRDVSGGRTDPSMPYETVLPSGLKMNLF
                     FYDAPISHAVAFEGLLSSGDNFAHRILGAFHQERDWYEIVNIATDGETYGHHHRFGDM
                     ALAYALNYIESNKLANLTNYGEYLERHPPTYQVEIFENTSWSCAHGVERWRSNCGCNS
                     GANPRWNQSWRTPLRESLDWLRDTITPAYEKKGKLLFNDPWAARNEYIQIILNRSPDC
                     VKQFLDKHAASNPSEDEKITMIKLLELQRHAMLMYTSCGWFYDDISGIESIQVIEYAG
                     RAIQLAEELFGNSFEPHFVELLDQAKSNIPNRGNGRSIYARFVKTAIANWEKLGAHYS
                     ISSLFEDYPPHNKIYYYSIDRQDYQHFESGKVKLAIGRVKITSEITTESAQLIFGALY
                     LGGHDIKGGVLQFQGEESYRGMVKELGDAFIKECFNDVLLLLDKHFEKSVYSLRSLFR
                     DEQRNTLNILLKSSLAESEAAFSQLYEQNAPIMRFVTSLGITPLKAFYSVADFVLNSK
                     LRRAFEDEKLDIENIKALLDEAKLQGVKLDAETLEYSLRMNIERMAEQLLASPTNISL
                     LQKLEDALDLTRSLPFMVNLWRPQNAYYKILKTVYPKILKRAEKEDESAKVWVSHFTS
                     LGEKLSIRVR"
     gene            3938..5437
                     /gene="malQ"
                     /locus_tag="VI935_03030"
     CDS             3938..5437
                     /gene="malQ"
                     /locus_tag="VI935_03030"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HLE24606.1"
                     /translation="MKRRGSGILLHITSLPSNFGIGDLGPSSCRFADFLSESKQSFWQ
                     ILPLNPTDLAFGSSPYSSPSAFAGNPLLISPESLVADGLLSSSDIEDAHLFSNERVDF
                     TAVTNYKEALLQKAFNNFKKKGSSNEFEEFCVKNSYWLEDYALFAVLKPHFNGVAWSE
                     WPIEIANRNETTLQELKNNLGDKVELEKFIQYIFFKQWFSLKSYCNNKKINIIGDIPI
                     YVNHDSADVWSNPEIFKLDKKKWPKFVAGVPPDYFSKTGQRWGNPVYRWNIIRKNDYQ
                     WWIKRMDHNLKLFDMMRLDHFRGFVGYWEIPADEKTAINGKWRKAPAKDLFTTLVKRF
                     NHLPIIAEDLGVITPDVIEVMNRFGFSGMKLLLFAFGDDFPNGSYLPHNYTKNCVVYT
                     GTHDNNTVRGWWRSEASPEDRKRVFKYIGREVSEENIHWEFIRLAMMSIANICIIPMQ
                     DILGLGEEAKMNRPSKTEGNWQWRLKPDQITSSQKGKLKEIIETYGRWY"
     gene            5559..5642
                     /locus_tag="VI935_03035"
                     /pseudo
     CDS             5559..5642
                     /locus_tag="VI935_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
BASE COUNT         1742 a         1115 c         1266 g         1618 t
ORIGIN      
        1 agcggtttat ggagaattcc cggatgtaca gaccatagct gaagagtcaa cggcatggcc
       61 tatggtttca agaccagtgt atgttggagg acttggcttc ggcatgaagt ggaacatggg
      121 atggatgcat gacacattag attatttctc aacagacccc atctacagga agtttcacca
      181 caatcagctt accttcagca tatggtacgc cttttcagaa aattttgtcc tccccctttc
      241 acacgacgag gtggttcacg gaaagggtgc actcatcgga aagatgcacg gagatgaatg
      301 gcaaaggaat gctaacgtga gggccttgtt tgggtatatg tatggacatc cgggaaagaa
      361 gcttttattt atgggaggag agtttggtca atggcgggaa tgggttcatg atgaaagtct
      421 agaatggcat tcacttcaat acccatttca gaggggtgta caattatggg tcaaagacct
      481 gaaccacctt tatagaactg aacatgcttt atatgagttg gattttagtt ttgagggttt
      541 tgagtggatt gattttcggg actctgagaa tagcattata agcttcataa ggaaaggcaa
      601 atataccaac gatatcattc tagtagtttg taacctcacg cctgctccta gatttaacta
      661 tcagttaggt gtcccgagag gaggcttctg gaaagagatt ttaaacagcg actccgtttt
      721 ctatggagga agcggactgg gaaattccgg tggtcttgaa gctaattacg ttagttcaca
      781 cggaaggcca ttctctttgt ttctgacctt accccctctt agtgttttat tctttaagag
      841 tgaaatataa gtgaagaact ctgcagcgag ttgtaaggta tcagcattta aagggaaggt
      901 tcagtttcaa tcttcatttg gaaacctaac ctgtcctgag aaagtcgaag ggaggtctcc
      961 gctacatttt gaattctgca tcaacgattc aaactcaatc tagctttagt ccgccatcaa
     1021 tgtcattccc gcatgtttcg agcgggaatc caaattgatt tagagttcct ggatgcccga
     1081 ttaaacttgt ccccgtatgt attaagcggg gagcactcgg acatgacatg caggaactct
     1141 aaaggtctcc gctacatttt gaatcagaga tgtattcttg agtagcggtg atctttagat
     1201 cgccacatag aaagaacttt agagtaatgg aagaaaacgt tttaatccct tcaattgatg
     1261 cgtgtaatat ttctagaact ttccttcgat tatacttaaa ctaatccaat gataaatcta
     1321 caagagagtt ataaaaacct ctctttaaac aagtgcatat aaagaatgga taagtatata
     1381 tgtattcatg gacactttta tcaaccgccc agagaaaacc cttggcttga agagattgaa
     1441 taccaggact cagcacaccc ctatcacgac tggaatgaaa aaataacagc agaatgttat
     1501 gctccaaacg gagcctctcg tattctagat catgaaggaa agattgtaga gattgtaaat
     1561 aattattcga agataagctt caactttggt cccacactcc ttggctggct agaaaaaaag
     1621 tcgccagacg tataccaagc tatattggaa gctgaccggg tgagtcaaaa taacttctca
     1681 ggacacggct cagcactagc gcaggtttac aaccatatta tcatgccctt agcaaatccc
     1741 cgggataaat acactcaggc gctatggggt atccgggact ttgagcgccg ctttggtcgc
     1801 aaaccagagg ggatgtggct tccagagact gcagtggatt tggagacatt agacatacta
     1861 gcaggccttg gtatgaggtt tgcaatcctt gcgccccatc aggctagtag ggtacgtaga
     1921 atcggtgcgc gtacatggcg cgatgtcagc ggtgggcgca cagatcctag tatgccttat
     1981 gaaactgtac taccgtctgg tttaaaaatg aatctatttt tctacgacgc acccatctcg
     2041 cacgccgttg cttttgaagg cttgctttca agcggtgata atttcgcgca tagaatccta
     2101 ggtgcattcc atcaggagcg cgactggtat gagatcgtta acatagctac agacggtgag
     2161 acctacggcc accatcatag gttcggagat atggctcttg cttatgcact caactatata
     2221 gaatcaaata agcttgcaaa cttgactaac tatggtgagt atctggaaag gcaccccccc
     2281 acatatcagg tagagatctt tgagaatacc tcatggagtt gcgctcacgg ggttgaaagg
     2341 tggcgaagca actgtgggtg taactctgga gcaaacccta gatggaatca gtcttggcga
     2401 accccgctaa gagaatcctt agactggtta cgagatacga ttactccagc ttatgaaaag
     2461 aagggaaaac ttttattcaa tgacccttgg gcagccagga atgaatatat acaaatcata
     2521 cttaaccgtt ctcccgattg tgttaaacaa ttcttggaca aacatgccgc tagtaatcct
     2581 agtgaagacg aaaagatcac gatgataaag ctcctagagc ttcagcgcca cgctatgcta
     2641 atgtacacga gttgtggatg gttttatgac gacatatccg gtattgaatc aattcaggtc
     2701 atcgaatatg cggggcgcgc cattcaactt gctgaagagc tttttggtaa ttcgtttgaa
     2761 cctcactttg tcgaactact agaccaggca aagagcaata ttcctaaccg aggcaacggg
     2821 cgtagtatct atgcaaggtt tgtaaaaact gctattgcca actgggaaaa attaggagcc
     2881 cactactcta taagctcctt gttcgaagac tatcctccac ataataagat ttattattac
     2941 tctatagacc gccaggacta ccagcatttt gaatctggga aagtaaaact tgcgatagga
     3001 cgagtaaaga tcacctcaga gatcacaact gagtcagcgc agttaatatt cggagcccta
     3061 tacctcggtg ggcatgatat aaaaggcggg gttctacagt tccaaggaga agagtcctac
     3121 aggggaatgg tgaaggaact aggtgatgct tttataaaag aatgcttcaa cgacgtcctc
     3181 ctgctcttag ataaacactt tgagaagtca gtctattcac tgaggtcgct atttcgtgac
     3241 gaacagcgta acaccttgaa tatcctcctg aaatctagtc tagctgaatc agaagcggcc
     3301 ttcagccaac tctatgagca aaacgctcct attatgcgtt ttgttacaag cttaggcatc
     3361 actccattaa aggcgtttta ttcagttgca gactttgttc ttaacagtaa actccgcaga
     3421 gccttcgagg atgaaaagct cgatattgaa aatattaaag cccttttaga tgaagcaaag
     3481 ctacaaggtg ttaaacttga cgcagaaaca ctcgagtact ctctaagaat gaatattgaa
     3541 aggatggccg agcaacttct tgcaagccct actaacatat ctctacttca gaaattagag
     3601 gatgctttgg atcttacacg ttctctccca tttatggtga acctttggag gcctcaaaat
     3661 gcctattata agattcttaa aactgtttat cctaagattc tgaaacgggc tgagaaagag
     3721 gatgaaagtg caaaggtctg ggtcagccac ttcacttctt taggagaaaa actctccatc
     3781 cgggttagat agatgagtcc agccaaggtc ttaaaacact ctctcttagt cttatttctc
     3841 aacagggagg aaaacagaat gcaggataca agatacatga tgcaagacaa actatttgtt
     3901 cgtgcatcat gaatcctgca tcatgtatca tttttttatg aaaagacggg gaagcggaat
     3961 actacttcat atcacctctc ttccatccaa ctttggaata ggagatctag gaccgtcttc
     4021 ttgtagattt gccgactttc tctctgaatc taaacagagt ttctggcaga tacttccttt
     4081 aaacccaact gatctggcct ttggaagttc cccatacagt agcccctctg cttttgccgg
     4141 gaaccctctt cttatcagcc ctgaatctct agtggcggac ggcctcttat caagttcaga
     4201 tattgaggat gctcaccttt tttctaacga aagggttgat tttacagccg taacaaatta
     4261 taaagaggca cttcttcaga aagcctttaa taatttcaag aaaaagggga gtagcaatga
     4321 gtttgaggaa ttttgtgtta agaactccta ctggcttgaa gattatgccc tttttgcagt
     4381 tctaaagcca cactttaatg gagtagcctg gagcgagtgg cctatagaaa tcgcaaatag
     4441 aaatgagaca acattacaag agcttaaaaa taatctcgga gataaagtag agcttgaaaa
     4501 atttattcaa tacatcttct ttaagcagtg gttttctctt aaaagctatt gtaataacaa
     4561 aaagataaat atcattggtg atattcctat atacgtgaat cacgatagcg cagatgtatg
     4621 gtcaaatccg gagattttca aattagacaa gaaaaagtgg cctaagtttg ttgcaggagt
     4681 tcctcccgat tattttagta aaactggtca gcgttgggga aatccggtct atagatggaa
     4741 cattattagg aaaaatgatt accaatggtg gatcaagcga atggatcaca acctgaaact
     4801 ctttgacatg atgaggcttg accattttag aggctttgtt ggatattggg agattcccgc
     4861 ggatgaaaaa acagccataa acgggaagtg gagaaaagcc ccagccaaag atctttttac
     4921 gacacttgtt aaacgtttta atcatcttcc aattattgct gaagaccttg gggttataac
     4981 acctgatgta atagaagtta tgaatcgctt tggattttca ggaatgaaat tacttctttt
     5041 cgcttttgga gatgattttc ctaatggctc atacttgcct cacaactaca caaaaaactg
     5101 tgtagtttac acaggtacac acgataacaa cacagttagg gggtggtgga ggtctgaggc
     5161 aagccctgaa gataggaaaa gggtttttaa atacataggg cgtgaggttt cagaagaaaa
     5221 tattcactgg gaatttataa gacttgctat gatgtctatt gcaaacattt gtatcattcc
     5281 gatgcaggat atacttggtc ttggcgaaga ggcaaaaatg aatcgtcctt cgaaaacgga
     5341 gggaaattgg cagtggagac ttaagccgga tcaaataacc tcttctcaaa aaggaaaact
     5401 gaaggagatt attgaaacct acgggaggtg gtattagttt gttatataaa attgatatgt
     5461 tgtttcttaa gttctttaga taatatgttt aatatccaat ctgagtgtat ttggatttgt
     5521 tagactataa ttaaatcttg atcagcagga agtatatgat gaaagtaaag gttggagtac
     5581 ataaagctga ggaaggggga tactgggcgg aagttccttc tcttcccgga tgttttacac
     5641 aggtgttagc taaaatttaa aagtgaactc tttaagaatt aaaagtgaac tatatttaac
     5701 tcactatttt taaccctttt gacatcaata agtttatcat t
//
LOCUS       DATDRL010000089         2439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3156733, whole genome shotgun sequence.
ACCESSION   DATDRL010000089 DATDRL010000000
VERSION     DATDRL010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3156733"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VI935_03040"
     CDS             complement(<1..947)
                     /locus_tag="VI935_03040"
                     /inference="COORDINATES: protein motif:HMM:NF039926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HLE24607.1"
                     /translation="MGSKVNEEILEQKLEELEKSKSWSPRVISKLEALIRSVDDFSLF
                     RINPLKFGSEKNISEYEVIDLFLYGTKCGIFKMNWELLCPGCGDVVESFSTLRNLDSN
                     YHCDLCQKDFEAALDDYIEISFTISPSIREIAFHNPETLSIEDYFYKYVFSQGAMFPD
                     GTKFVDLVKKSAKIITYLEPKEKKKFELEIPPGFLVGCDRLNHGELFFHVNGESKAEI
                     QHFSFKLVDGKYEPDKGELFPGKFVFDFENHTNKRGALLLIHFPNLPPESLFYKPFLS
                     GKRLLTTQTFRDLFRTEIVQGNEGIGIKDITILFTDLKGS"
     gene            complement(1143..1634)
                     /locus_tag="VI935_03045"
     CDS             complement(1143..1634)
                     /locus_tag="VI935_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HLE24608.1"
                     /translation="MTKNSIFITLIIACLSISFLLTGCSAYEQVKEFIHPDYIKCYVA
                     KVIDVDTFDCQLSDVNIERVRLIGIGISESITDNATDFSKSYLRRGTPVKLELDEQIR
                     DRDGRILAYVYLPGGKMLNTLLIQEGFGQVIINPPNIKYKDLFLRVETEAREQRKGIW
                     KGK"
     gene            complement(1707..>2439)
                     /locus_tag="VI935_03050"
     CDS             complement(1707..>2439)
                     /locus_tag="VI935_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HLE24609.1"
                     /translation="LSQVSSFGVALAFYLQGFSALSLFQSLIDPFALSLVMTGAAALF
                     FALNRVFFRISQEGVIGFGYLLASGIVVIIADRITHEAHDIADILFGSAVVVDPKEVY
                     IVPAVASVAILINWIFFKDFIFVSFDPETARLFKYPVRILNTVLLLTVGVVIAVTTRA
                     LGALPVFALAVLPALTALFLTEKLNWVFVIAVLIGILSAGFGYYFSFILSLPTGASIT
                     TCASLFFLLGVIWQQGRLLSRSKFG"
BASE COUNT          814 a          510 c          416 g          699 t
ORIGIN      
        1 gatcccttta agtctgtaaa aagaatagtg atatctttga ttccgattcc ctcgtttccc
       61 tgaacgattt cggtgcggaa aagatcccta aaggtttgtg tagtgagaag cctctttcca
      121 gaaagaaagg gcttatagaa tagagactct ggcggtaggt tagggaagtg gattaataaa
      181 agcgctcctc ttttgtttgt atgattctcg aaatcgaaaa caaatttgcc agggaaaagt
      241 tcacccttat caggctcata ttttccatca accaatttga aagaaaaatg ctgaatctca
      301 gctttggatt cgccatttac atgaaaaaat agctccccat ggttaagccg atcacacccc
      361 accaaaaaac ctggaggaat ctcgagttca aattttttct tttctttcgg ttctagataa
      421 gtgatgattt ttgccgattt ctttaccaaa tcaacaaatt ttgttccgtc tggaaacata
      481 gcgccttgac taaatacata tttataaaaa taatcctcta tcgaaagggt ttcggggtta
      541 tgaaaagcta tctccctgat tgaaggagaa atggtaaagc tgatctcaat ataatcatcg
      601 agcgcagctt caaaatcctt ctgacataaa tcacaatgat aattggaatc gagatttctc
      661 agcgtgctaa agctttcaac tacgtcccca catcctgggc ataaaagttc ccagttcatt
      721 ttgaaaatcc cacacttggt gccataaaga aataggtcaa taacctcata ctctgagata
      781 tttttttcac ttccgaactt caagggattt atcctgaaaa gtgaaaagtc atccacggaa
      841 cgaattaaag cctccaactt tgaaattacc ctaggactcc atgatttcga tttttcaagc
      901 tcttcgagtt tctgttctag aatttcttcg ttgactttac tccccatctt tctcaccttt
      961 caagatatta aagttaatat atattaagca ataatctttc aaaagaatta cctgtcgctt
     1021 atcagtttac aatttagaaa tgtccaaata tataaatact gattttttaa agattttaat
     1081 gccttccact tctaataacg aaggcgggac atccttgtcc cccgcagagg tttctattct
     1141 tgctatttgc ctttccagat ccctttacgt tgctctctag cttctgtctc taccctcagg
     1201 aacaaatctt tatactttat attaggcgga tttattataa cctgaccaaa tccttcttga
     1261 atgagtaaag tattgagcat tttcccacca ggtaaataaa cataagcaag aatccgtcca
     1321 tccctatctc taatctgttc atcgagttca agcttaactg gagttcccct tctaaggtag
     1381 gacttggaaa agtctgtcgc attgtctgta atagattctg atattccgat accaattaac
     1441 ctaacccttt ctatattcac atctgaaagt tgacaatcaa acgtatcaac atcaatgact
     1501 tttgccacgt agcatttaat gtaatcaggg tgaataaact cttttacctg ctcgtaagcg
     1561 ctacaccctg tgagaagaaa agatatagac aaacaagcaa ttattagtgt gataaatata
     1621 gagtttttag tcatgaatta ccaagtggag ttttatttag tttatttaca aagctagaaa
     1681 gcgttatatt ctatataaga cgctcgttac ccaaacttag acctggataa aagcctccct
     1741 tgctgccaaa tcacccctaa aaggaaaaat aaggatgcac aggtcgttat agaagcaccg
     1801 gttggaagag ataaaataaa agaaaaataa tagccaaaac ctgctgataa aatccctatt
     1861 aaaactgcga taacaaaaac ccaattcagt ttctcagtta aaaaaagcgc tgtaagagct
     1921 ggaagaacag ccaaagcaaa tacaggaaga gccccaagag ctcgtgttgt gactgctatc
     1981 acaaccccaa cggtgagcaa taaaactgta ttaagtattc ttactggata cttaaataac
     2041 cgtgccgtct cagggtcgaa agagacaaag ataaaatcct taaagaatat ccagttaatg
     2101 agaatagcaa ctgatgctac agccggcact atataaacct ctttcggatc tacaactaca
     2161 gcacttccga aaagaatgtc agcgatgtca tgagcttcgt gtgtgattcg gtcagctata
     2221 atgactacaa ttccagaagc aagtaaatac ccgaaaccta ttactccctc ttggcttata
     2281 cgaaaaaaaa ccctgttcag agcgaaaaaa agggcagcag cccctgtcat aaccaatgat
     2341 aaggcaaagg gatctatcaa agattgaaat aaacttaaag cagaaaaccc ctgaagatag
     2401 aaggcaagag ccacaccaaa acttgagacc tgagagagc
//
LOCUS       DATDRL010000090         5987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2141952, whole genome shotgun sequence.
ACCESSION   DATDRL010000090 DATDRL010000000
VERSION     DATDRL010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141952"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="VI935_03055"
     CDS             complement(<1..1661)
                     /locus_tag="VI935_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HLE24610.1"
                     /translation="MSRTISDRLLEVARTSFVGRRRELAVLCGAIEAAELPFVVAFIH
                     GLGGIGKSRLLQATLSSVGPEIRSIVMDCREIEPTTKGFLAALSTALGMEQSEPELRS
                     VVAHLGETAKRTVLALDTYETFGLMDTWLRQVFVPALPETVLTVIVGRQAPNAAWITT
                     PGWESLFREIKLQELPDDDAHEMLKLRGLKQSQVERANRFARGYPLALELAAAALRTQ
                     PDLEIASGPPPKVLQQLTHAFLAGLPSEITEAVEAASTVRHITEPVLKALLTMSHVKE
                     VFEKLQGLPFVNATGEGLILHDVVRDTIANDLVRRDPERYRTYHRRAWRFFTSEPHRT
                     ATRNLWQYTADLLYLIQNPNIREAFFPRGASDYIVEPATFSDGKDICDIAAASEPEEA
                     ARFIQRWWDKHPKTFSVARTRDVRVAAFYILFEPTDVDPDLLAEDPLTAAWLQHLRIN
                     PVARGERVLFLRRWLTRLIGEAHSPALAACWLDVKRTYMELRPSLRRLYGTVTDPAPF
                     LPMLGPLGFVPLEETNVVLGGVTYHTAMLDFGPSSVDGWLRGLIGA"
     gene            complement(1956..3512)
                     /locus_tag="VI935_03060"
     CDS             complement(1956..3512)
                     /locus_tag="VI935_03060"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HLE24611.1"
                     /translation="MSHQHIDKKIETKLEHKYSTLFKLCHMMSYERDLNTLLDFLVKK
                     SADVVDADRATIFLFDEKRNELWSKIAMGLTDVIRFDARLGIAGEVLKSGKIFNIEDA
                     YKHPKFNPDVDKRTGYTTKTLLCVPMKDINGKPIGVLQTLNKKEGLLFTTEDVEVLEI
                     FASHAAIAVENARLIGELEGSKARLQQENVILREKTRGRFFVSNLIGSSSRIQDIVKL
                     IEKIADSPLNVLITGESGTGKELAARTIHYNSSRFERPFIEINCAALPESLLESELFG
                     IEKGVATGVEKRVGKFEMANGGTLFLDEIGDMSLPAQAKLLRVLQERKVERVGGRSMI
                     DIDVRVLAASNKDLKSEIEKERFREDLYYRLNVVHIHMPPLREMKEDIPFLAKYFLTS
                     FTSELGKGAMIFSNEAMDCLMKYSWHGNVRELENEVKRAAILAEDNVINEKDLSENVR
                     GIPESRGGSRTAPTEEGIQSLKETVEEIEIRMIKEALEKSGGNKQKASEILGITRQGL
                     IKKIKRYGLS"
     gene            3746..3892
                     /locus_tag="VI935_03065"
     CDS             3746..3892
                     /locus_tag="VI935_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24612.1"
                     /translation="MRKLIAITTLGLALSFPTLAFASDCYKAFGVKLPMVKSKVENEV
                     MVET"
     gene            3904..4077
                     /locus_tag="VI935_03070"
     CDS             3904..4077
                     /locus_tag="VI935_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24613.1"
                     /translation="MSFYSTPQTANTTALLITADQKSDDEYISVFGVQITRSSRLKGE
                     LAPPKCPALPVSS"
     gene            4152..4355
                     /locus_tag="VI935_03075"
     CDS             4152..4355
                     /locus_tag="VI935_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24614.1"
                     /translation="MTITITPFIIDPNCDCTVLSTGHEKEEHTKRKHLPITYLVIYLD
                     DKYISYTSSKELAQKIKFGWRKC"
     gene            4482..5102
                     /locus_tag="VI935_03080"
     CDS             4482..5102
                     /locus_tag="VI935_03080"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HLE24615.1"
                     /translation="MTEKKVKWNWTADYIQGCNCDWGCQCNFNAPPTKGHCEGMSAWH
                     IRKGRYGKVVLDGINCAVAAKWPGQIHEGNGTATVYIDERANLEQRDALIMIMSGQAG
                     GLPFELIATTFSTLLEPRYVSIDFKVAGKDSSITIDNLMNVILEPMTNPVTGAIHDAQ
                     VVFPNGFIFKKGETLNTKECVVNDGPISFSYPGKNGHYAVVKYSGL"
     gene            complement(5083..>5987)
                     /locus_tag="VI935_03085"
     CDS             complement(5083..>5987)
                     /locus_tag="VI935_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLE24616.1"
                     /translation="IIEELAWGDIGTALALINGPGLGGYAILELGNENQKRKYLSFFS
                     NPYGYNMRCAFCLLENQPDFTLENMQTVATLVDGDFLLSGKKFCVMNGDRADFYIVFA
                     RIEGKGGIEAFIIEKGTTGLQTGKVHEKLGLNSVPTADIILDGCRVPKENLLNGASEL
                     GKALESIFIREQIISTALMVGCARVASEYAFKYAFERKAFGVPIYQHQGLSFMMAEMA
                     VEVDASRLMLWQAAWAFDKGKEEAPFLTEKAFLQAKRMSSKVTSDAVQILGGHGFIQD
                     HPVEKWMRDAKTLAVLVQSIDPNI"
BASE COUNT         1621 a         1369 c         1283 g         1714 t
ORIGIN      
        1 gcaccgatca gccctctcag ccagccgtca acactggagg ggccgaagtc gagcatggcc
       61 gtgtgatagg taaccccacc gagcactaca ttcgtctcct caagcgggac gaagccaaga
      121 ggacctagca tgggaaggaa gggggctggg tctgttacgg ttccgtagag ccgtcgcaaa
      181 ctaggtcgaa gctccatgta ggtgcgcttg acgtcgagcc agcacgcagc cagagccggt
      241 gagtgagcct cgccgattaa gcgggtaagc cacctccgga ggaagagcac acgctccccg
      301 cgagcgacgg gatttatacg taggtgctgc aaccatgcgg cagtgagggg atcttctgcc
      361 aggaggtcag ggtcaacgtc agtgggctcg aagagtatgt aaaacgctgc tactctgaca
      421 tcacgagtcc ttgcgacact aaaggtcttg gggtgcttgt cccaccagcg ttggataaac
      481 cgggctgctt cttcaggctc tgaagcggca gcgatatcgc agatatcctt accgtcactg
      541 aatgtggccg gctctactat gtagtcactg gctccacgtg gaaagaacgc ctccctaatg
      601 ttggggttct gtataagata gagcaagtcc gcggtgtact gccagaggtt tcgagttgct
      661 gtccgatgcg gctcgctggt gaagaaacgc catgcgcgtc ggtggtacgt acggtagcgc
      721 tcagggtcac gccgaaccag atcattcgcg attgtgtctc gcacgacatc gtggagaata
      781 agtccttctc ccgttgcatt gacgaatggg aggccctgta gtttctcaaa tacctccttt
      841 acatgtgaca tggtgagtaa cgccttcagc actggctcgg taatgtggcg gaccgtggag
      901 gcggcttcga cagcctccgt gatctccgat ggaagacccg ccaagaaggc gtgagtcaat
      961 tgttgcaaaa ccttcggagg aggaccgctg gcgatctcca ggtcgggctg agtgcgaagg
     1021 gcggctgcgg ctagctccag cgcgagagga tagccgcggg caaagcggtt ggctctctca
     1081 acctgagact gcttaaggcc acgtaacttt agcatctcgt gggcgtcatc gtcaggaagt
     1141 tcctgcagtt tgatttcgcg aaagaggctt tcccatcctg gagtagtaat ccaggccgca
     1201 ttgggcgcct ggcgaccgac aataaccgtg agaaccgtct ccggcagagc cggcacaaaa
     1261 acttggcgga gccatgtgtc catgagtcca aacgtctcat aggtgtccag agcgagcaca
     1321 gttcgctttg cagtttcacc taagtgagca acaactgagc gtagttcagg ttcagattgc
     1381 tccattccga gtgcagtgct aagggctgca aggaagccct ttgtggtggg ctcaatctca
     1441 cggcagtcca tgacgatcga gcggatttcg ggaccgacag aactcaacgt tgcctggagt
     1501 aggcgagatt taccaattcc gcccagtccg tgaatgaagg cgacgacgaa tggtagctcc
     1561 gcggcttcga tagcaccaca gagcacggcg agttctctcc gccgtccaac gaatgaggtt
     1621 ctggcaacct ctagtagtcg gtctgaaata gtacgagaca tgacagccct atttttttgc
     1681 aggatggcga atggattttc acgttttagt tttacgaaac cttcttgtag taaaggagta
     1741 gggtagaggg aatgtaaaag tttttcttag gtacgtagaa aatcgctgat agctggaaat
     1801 acctcatcat aaattctaac ccatccgctg ccgtgatttg actatcattc tagaatattt
     1861 cgaatataaa agtcaaggga aaatttatca tgatacagga ttcgtgatag atgattcatc
     1921 ttgtgtcctg caccctgtgt atcgtgcatc atgcatcatg ataacccata tctttttatc
     1981 ttttttatca atccctgcct cgttatcccc agaatctcag atgccttttg tttatttccc
     2041 ccgctttttt ctagcgcctc ttttatcatg cgaatttcga tttcttctac ggtttcttta
     2101 agagattgaa tgccttcctc agtaggggcg gttcgcgaac cgcccctact ttccggtata
     2161 cctctcacat tctctgaaag gtctttttca tttataacat tatcttcagc caatattgcc
     2221 gcacgtttta cctcgttctc taattctctt acatttccgt gccaactgta tttcataagg
     2281 caatccattg cttcgttgga aaaaatcata gcacccttac caagttcgct tgtaaagcta
     2341 gttaaaaaat attttgcaag aaatggaata tcctccttca tctcacgaag cggaggcata
     2401 tgaatatgga caacatttag acggtagtac aagtcttcac ggaacctttc cttttctatc
     2461 tcactcttca agtccttatt tgaagctgcg agaaccctta catctatatc aatcatgctt
     2521 cttcctccaa ccctctccac ctttctctcc tgaagaactc ggaggagttt ggcttgagct
     2581 ggcagactca tgtccccgat ttcatccaga aataatgttc cgccatttgc catctcaaat
     2641 tttcctactc tcttttctac ccctgttgcc actccctttt ctattccaaa gagctcactt
     2701 tcaagtagac tctctggtaa tgctgcgcag ttaatctcta taaatggtct ttcgaaacgt
     2761 gagctattgt agtgtatcgt acgtgcggca agctcctttc cagttccgct ttctcctgta
     2821 attagaacat taagaggact atcagctatc ttttctatga gttttactat gtcctgtatt
     2881 cttgaacttg atcctattaa gttactaaca aaaaacctcc cccttgtttt ttctcttaga
     2941 ataacgtttt cttgttgaag ccgtgccttt gacccttcga gttctcctat gagtcttgcg
     3001 ttctctacag ctatggctgc atgagaggca aaaatctcca agacttccac atcctccgtt
     3061 gtaaataata acccttcttt tttatttagt gtttgtaaga ctcctatagg ttttccgttt
     3121 atatccttca ttggtacaca gagaagagtt tttgtggtat atccggttct tttatcaaca
     3181 tctgggttaa atttcggatg tttgtatgcg tcttcaatat taaaaatttt ccccgattta
     3241 agcacttctc cagcaattcc tagccttgca tcgaagcgta ttacgtctgt taaacccata
     3301 gctatctttg accaaagttc atttctcttt tcgtcaaaaa gaaatatagt tgcccggtct
     3361 gcatcaacga catctgctga cttcttaacc agaaagtcta aaagtgtatt caagtccctc
     3421 tcataactca tcatgtggca gagtttgaaa agggttgagt atttatgctc taattttgtt
     3481 tctatctttt tgtctatatg ttgatgactc attaaatagc tatcaacaac agtatattat
     3541 agcgtcccta taaccgtcaa ctttgtttac gacactgttg atagcattta cgattcaagt
     3601 ctaattcgta atttaattat acaagaccac aaactagaat agcattgttt acaatgggtc
     3661 actgataata cgtaatttcc tctaatgact ggcacacctg ttgcgaataa gcataataaa
     3721 aaatcaagaa aggaggtgac acaagatgag aaagctaatc gctatcacaa cgcttgggtt
     3781 agcccttagt ttccctacac tagcgttcgc ctccgattgc tataaggcat tcggcgttaa
     3841 acttccaatg gttaaaagca aggttgaaaa cgaggtaatg gtggaaacgt agagaaggat
     3901 tttatgagct tttactctac tccacaaaca gcgaacacaa ccgctttgct aataacggca
     3961 gaccagaaat cagacgatga atatatctct gtattcggtg ttcagattac acgtagttca
     4021 agattaaaag gggaattggc tccaccgaaa tgcccagcac tgcccgtatc ttcatgattt
     4081 acccctaatc gaagagaaac tggattgagg aaactctcga gattttcttt taaagaaaga
     4141 aggtgataaa aatgactata acaataaccc cattcataat tgacccaaac tgcgactgca
     4201 cagtattgtc gaccggacat gaaaaagaag aacacactaa aagaaaacat ctgccgatta
     4261 cttacttggt aatctatctc gacgacaaat acatatctta tacatcgagt aaagaactgg
     4321 ctcaaaagat aaagtttgga tggagaaaat gttaaaggac agtctttatg attaaaaaga
     4381 taaggtgtta ataatttttt gcaatcggtt attaaatgtt agattctaaa aatatgaagg
     4441 tttaataaaa tcagtttaaa atctaactga agaggtaaaa aatgactgag aaaaaagtga
     4501 aatggaattg gacagctgac tatattcagg gctgcaattg tgattgggga tgccaatgta
     4561 attttaatgc tccgccaact aagggtcatt gcgaggggat gtcggcctgg catatacgaa
     4621 agggtcgtta tggaaaggta gttctagatg ggattaactg tgccgttgcc gcaaagtggc
     4681 ctggacagat acacgaggga aacgggacag ctacagtata tattgatgaa agagctaacc
     4741 ttgagcaaag ggatgcactt attatgatta tgagcggcca ggcagggggg cttccttttg
     4801 agttaattgc aacgacgttt tctacattac ttgagccgag gtatgtttca atagatttca
     4861 aggttgccgg aaaagatagc agtatcacaa ttgacaacct tatgaatgta atcctagaac
     4921 ccatgacaaa tccggtaact ggagctattc atgatgccca agtagtcttt cctaatggat
     4981 ttatatttaa aaaaggtgaa actttaaaca ctaaagaatg tgtagttaat gacggcccca
     5041 taagcttcag ctatcctggt aaaaatggcc actatgctgt tgttaaatat tcgggtctat
     5101 agattgtact aacaccgcaa gagttttggc atccctcatc catttctcaa ccgggtggtc
     5161 ttggataaat ccatgacctc ccaagatctg tacagcatcc gatgttacct ttgaactcat
     5221 tcttttagcc tgtaagaatg ctttttctgt aaggaaagga gcctcttcct ttcctttatc
     5281 aaatgcccaa gctgcctgcc ataacatcaa tcttgaggca tccacttcta ctgccatctc
     5341 agccatcatg aatgaaagcc cctgatgttg atagattgga acaccaaatg cctttctttc
     5401 gaaagcatat ttaaatgcat attcactagc aactctggca catccaacca ttaaggcagt
     5461 agatatgatt tgttctctta taaatatgct ttcaagtgct tttccaagtt ctgatgctcc
     5521 attgaggagg ttctctttag ggactcgaca cccatccaag attatatccg ctgtcggaac
     5581 ggagttaagg cccagttttt cgtgtacctt gcctgtttga agtccagttg ttcctttctc
     5641 tattataaaa gcctctatcc cacctttacc ctctattcta gcaaatacga tgtaaaaatc
     5701 tgctctatcc ccgttcatca cacaaaactt ctttcctgaa agtaggaagt ctccatcaac
     5761 tagggtggca actgtttgca tattttcgag tgtaaaatca ggttgatttt caagaagaca
     5821 aaaagcacac ctcatattgt atccgtaagg attcgagaaa aatgaaaggt atttcctttt
     5881 ttgattttca ttcccaagct cgagtattgc ataccctcca agcccaggcc cattaattaa
     5941 agcaagagcg gttccaatat ctccccaagc tagctcctcg attatga
//
LOCUS       DATDRL010000091         2658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_312909, whole genome shotgun sequence.
ACCESSION   DATDRL010000091 DATDRL010000000
VERSION     DATDRL010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312909"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VI935_03090"
     CDS             <1..374
                     /locus_tag="VI935_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HLE24617.1"
                     /translation="FDDFTQRDVRDDRNLVLEHGKPLIFGKNRDKGLRFKDGMVEIAE
                     FEHANPPEDITVFDETNLSLAFALSKLYWPRFPVPMGVIRRVEQPSFEELIYGQIDMA
                     KQKFSTDLNKLIYSGDVWEVK"
     gene            612..1190
                     /locus_tag="VI935_03095"
     CDS             612..1190
                     /locus_tag="VI935_03095"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HLE24618.1"
                     /translation="MAIKVKVIQPPKLSFLERIYIPEIIKGLKTTLMHILNFHPITVQ
                     YPEEVKQLPPRYRGLHILPADDEGKIRCVACKLCEVACPTQAISIVAEASDEYGIERK
                     PKIYNIDFMRCVFCGFCVEACPCDALRMGMQYELASYNRAGLIHTKEVFFDPNVKSRA
                     PRDNANFLYERERGEILDEPHEVEKVRNASDI"
     gene            1314..2348
                     /locus_tag="VI935_03100"
     CDS             1314..2348
                     /locus_tag="VI935_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HLE24619.1"
                     /translation="MKAVVVYEHGGIDKLRYDEIPNPEVRANDVLVQVKACAMNHLDI
                     WVRKGLPHLKIKYPHVLGSDVAGIVAEVGNDVRGIEIGTPVLVSPGITCGKCVYCLSG
                     RDNLCREYAILGENCPGGCGEYVSVPKENILPMPRNVSFEQGACIPLVFLTAWHMLVS
                     RAEIKQGEAVLVHAAGSGVGSAAIQIAKLFGATVITTAGTDEKAEKAKTLLGADYGIN
                     YNTQDFLAEVRKITEKRGVDIIIDHTGVVNWEKNILALTMGGRMVICGSTSGFEVKTD
                     LRQVFFKRLSILGSTMGSKGDLFEVIKHVESGKLKPVLYKVLPMSQAAEGHKFMENRE
                     VFGKIVLIPS"
     gene            complement(2364..>2658)
                     /locus_tag="VI935_03105"
     CDS             complement(2364..>2658)
                     /locus_tag="VI935_03105"
                     /inference="COORDINATES: protein motif:HMM:NF018534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein C"
                     /protein_id="HLE24620.1"
                     /translation="EGTQPSSIELNTIHVNPHIIYPSERTIIALDPDIPERHHLIFFE
                     AKASNTNFQWLLNTKKIGTSSKRVSWKPKKGRYVLSIVDKQNRIIDSVEFEVR"
BASE COUNT          828 a          456 c          590 g          784 t
ORIGIN      
        1 tatttgatga tttcacacaa agagatgtaa gagatgacag aaatcttgtt cttgagcatg
       61 gcaaacctct tatattcggt aaaaacagag ataagggttt gagattcaaa gatggcatgg
      121 ttgaaatagc agagtttgaa catgcgaatc cacccgagga tattaccgtt tttgatgaaa
      181 cgaacctttc attagcattt gctctttcaa aactctattg gccgcgattc ccagtaccca
      241 tgggggtaat tagaagggta gagcaaccga gttttgaaga attaatctat ggacagattg
      301 acatggcaaa acaaaaattc tcaacagacc taaacaaact tatttactcg ggtgacgtct
      361 gggaagttaa gtaaagtcac atcaaaagat aaagtaaaaa aggctcctaa caaatggaag
      421 ccttagaatt cgtaagccag aaacaagtta acttaaacac tagaataaga atatttcata
      481 ccgctttatc cagtcaatct agttcaattt tttttccaaa tataggcttt actatcgtgt
      541 tgttaatcca gaacttttat tgccttcaat ttgtgttaaa ttataaaaag acagtcattt
      601 gaggaacagg tatggctata aaagtaaaag ttatccagcc gccaaagttg agtttccttg
      661 agaggatcta cattcctgag ataatcaagg gtttgaaaac aaccctgatg catatcctta
      721 actttcaccc tataacagtt caatatcctg aagaggtaaa gcagcttccc ccaaggtata
      781 ggggtcttca tatccttcca gcggatgatg agggtaaaat aaggtgtgtt gcatgcaagc
      841 tatgtgaggt tgcgtgtccc actcaggcta tatcaatagt agctgaggct tcggatgaat
      901 atggaatcga aagaaaacca aagatttata acatagattt tatgcgatgt gttttctgcg
      961 gattctgtgt tgaggcttgt ccctgcgatg cccttagaat ggggatgcaa tacgagcttg
     1021 catcatacaa tagagcaggg cttattcata caaaagaggt tttctttgat ccaaatgtaa
     1081 aaagtagagc ccctagggat aatgctaatt tcttatatga aagagaaagg ggtgaaattt
     1141 tagacgaacc acatgaggtc gagaaggtca ggaatgcttc agatatttga ttaaattgta
     1201 gtttaaaaac catagccaca gagctcacag agaaaagaaa atattttttg tttctctttg
     1261 tgttctatgt ggcaaatctt ttcaaattcc acaatcgaaa gggggtttaa attatgaaag
     1321 cggttgtagt ttatgaacat ggtggaattg ataaattaag gtacgatgag attccaaacc
     1381 cagaagtccg cgcaaatgac gttctagtgc aggtaaaagc ctgcgcgatg aatcaccttg
     1441 atatatgggt aagaaaaggt cttcctcacc ttaaaataaa atatcctcat gttctaggtt
     1501 ctgatgtggc tggaattgtt gcagaagtag gaaacgacgt tcgtgggatt gaaataggaa
     1561 ctcctgtttt agtctccccg ggaataacct gtggaaagtg tgtttactgt ttaagtggcc
     1621 gtgataatct ctgcagagag tatgcaatct tgggcgagaa ctgtcccggt ggctgcggtg
     1681 agtatgtgtc tgttccaaaa gagaatattc ttcccatgcc aagaaatgtt tcatttgaac
     1741 aaggggcttg cattccactt gtctttctta cagcatggca catgcttgtg agtcgtgctg
     1801 agattaaaca aggggaggca gttttagtcc atgcggcagg aagcggtgtc ggaagcgccg
     1861 caatccaaat tgcaaagctc tttggggcaa ccgtaattac tactgcagga acagacgaaa
     1921 aggcagaaaa ggcgaagacg ctccttggtg ctgattatgg aatcaattac aacacacaag
     1981 atttccttgc tgaagtaagg aaaataactg aaaaaagggg tgtggatatt attatagacc
     2041 acacgggggt cgtaaactgg gaaaagaaca ttcttgcact aacgatgggc ggcagaatgg
     2101 ttatttgtgg ctcaacttct ggatttgaag ttaaaacaga cttaaggcag gtatttttca
     2161 aaagactttc cattcttggc tcaactatgg gaagcaaggg tgatttattt gaggttataa
     2221 agcatgttga atctggcaaa ctgaaacccg ttctctacaa agttcttcct atgtctcagg
     2281 ctgcagaagg ccacaagttt atggaaaaca gagaggtctt tggtaaaata gtcctaattc
     2341 catcgtgatt ctttctaggc tggtcatctc acttcaaatt ctactgagtc aataatacgg
     2401 ttttgtttat ctacgattga tagcacatat ctaccctttt taggtttcca tgaaactctt
     2461 ttactagaag tgcctatttt tttagtattt aataaccact ggaaattagt attagatgct
     2521 tttgcttcaa aaaagattag gtggtgtctt tcgggaatat ccggatctaa agcgattata
     2581 gttctctctg acggatagat aatatgggga tttacgtgga ttgtatttaa ttcaattgaa
     2641 cttggttgtg tgccctct
//
LOCUS       DATDRL010000092         8476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3746826, whole genome shotgun sequence.
ACCESSION   DATDRL010000092 DATDRL010000000
VERSION     DATDRL010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3746826"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VI935_03110"
     CDS             <1..182
                     /locus_tag="VI935_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24621.1"
                     /translation="SQGLNRFLALRLLVSKIEEKILGKKSPEQLKRDKNRRQKKRRYR
                     RARKKLASISASKQI"
     gene            complement(684..1064)
                     /locus_tag="VI935_03115"
     CDS             complement(684..1064)
                     /locus_tag="VI935_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24622.1"
                     /translation="MHSRNQYLKQIRVEYLKTRSKKGRGSLLDESEKRTGLNRKYLIR
                     KIRVLSNLDKKKVKVRRRKQVYDGEVTTALVKCWEIFDYPCGQRLKPLLSEEAKRLRR
                     LGELICSDEVWDKLNQYACLNPRP"
     gene            1382..1918
                     /locus_tag="VI935_03120"
     CDS             1382..1918
                     /locus_tag="VI935_03120"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HLE24623.1"
                     /translation="MSLWERIEHCINTWADKSPPKWWKKKFSIGSWLILRCILLLPLI
                     VLSFRVPSLWSVWSFFTLVIIADILVVNTSVCLFTRNPRSKIRAIVFTLFAFIQLGLA
                     FSVYYATLSTKCMLNQSLGIVGTIYFSFVTMLTLGYGDVLPTHEVAQLTTIIQVNIGL
                     YFISILIAYVVGKLAQDK"
     gene            1952..2656
                     /locus_tag="VI935_03125"
     CDS             1952..2656
                     /locus_tag="VI935_03125"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HLE24624.1"
                     /translation="MEVLGIIPARQGSKGIKYKNIIPLLGKPLISYTIMAAKKSKMIS
                     RLIVSSDDPKILAIAESEGVKTPFLRPESLATGETPSADVAIHAFQKVQSIEGKKYDA
                     IILLQPTSPLRTTYDIDNAINLLEEKDADSVISVMKLEKCHPFKLLRVENEKLKPLYP
                     EKWKGSIRRQEMPPIYITNGAIYCIKSHLLIKNRTFWSDNNIPYIMPPERSIDIDSVI
                     DLKLAEILLKERIENE"
     gene            2649..3686
                     /locus_tag="VI935_03130"
     CDS             2649..3686
                     /locus_tag="VI935_03130"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HLE24625.1"
                     /translation="MNKIVLDNRTIGCNEPVLIIAEIGVNHGGCLENALNLIDAAREA
                     CVDAVKLQSHLADEEVILENSSQSNKYLEAYKRWKRFELSEYQHRIIKEYAEEKGLIF
                     FSTAFSMAAVEMLNNIRVPLFKLGSGDITDHQLIELIASKGKPIIISTGMSTEKEIED
                     TVNLIRKYNVPYAIMHCTSLYPCPYECIDLLVIKELRAKYNVPVGLSDHTPTIYTAVA
                     STVLGVNIIEKHFTLSKRLYGPDHKVSLTPEEMKELVQGVRAVEKALKTFNKANSTEI
                     QEVREMYLKGIVARNDLTKGSTIRQEDLICKRGAKGIPANRIGDIVGKHVKRAISKNS
                     SIFPEDIIGLE"
     gene            complement(4223..5236)
                     /locus_tag="VI935_03135"
     CDS             complement(4223..5236)
                     /locus_tag="VI935_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24626.1"
                     /translation="MSKKYWQIAGGSFGRDYSDLFIKFGMAFVGGDSQTMKEVEKGDI
                     VLLKKGLYQIVAVGEVAERDGKHRDCGDKSWLRDIDGWDLQAYCYVDWRVPDTPIQTN
                     GLTRATIQKVPQDKHKQLADSILQLPVCAYKLEPSKTELIDDNQILKFLISEGLRPSA
                     ADDLTNTFRRIRLLADYYYHYPKWEDIREHETRTFLIVPLLLALGWAEQQIKIELPSS
                     KGKMSGSKGKIDIACFSKAYLRKDDECALIIETKGFSSGLDYAPEEARGYVKAFPSCQ
                     ILVVSNGYCYKTYIRKDSGDFSTMPSAYLNLLNPRDKYPLDPDNVKGALDVLRWLLPT
                     ALR"
     gene            complement(5464..5540)
                     /locus_tag="VI935_03140"
     tRNA            complement(5464..5540)
                     /locus_tag="VI935_03140"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5504..5506),aa:Pro,seq:cgg)
     gene            complement(5750..6376)
                     /locus_tag="VI935_03145"
     CDS             complement(5750..6376)
                     /locus_tag="VI935_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLE24627.1"
                     /translation="MLKINDSITLSWLGHASFKLKAGERIIYFDPWKVKKGEGADLIL
                     ITHSHFDHLSPDDVRHIQKKDTVIVTTKDSDSNLKGDVRIIKPGDKITVGDIEIQAIP
                     AYNIGKSYHPKANGWVGFIVTISGKRIYHAGDTDAIPEMKKLSVDVALLPVGGTYTMT
                     AEEAAEIANEFKPISVIPMHWGTIVGSKADAERFKKLFKGDTNILQPE"
     gene            complement(6541..7362)
                     /locus_tag="VI935_03150"
     CDS             complement(6541..7362)
                     /locus_tag="VI935_03150"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HLE24628.1"
                     /translation="MKMLKSNTSAIFLMVFIFVIYSFAYMGTVGAVSAEDIKVPPAPQ
                     LVTDAWMLIIGFEVDPAAIKAVLPQGLEPHPNNMVVMNMYTVPDPSQTSGFGAYTLTY
                     LTVQVKDQDSYTMGSTTGEPGRYFVSYFNSSELMRKFTKEVGIPAKPGNTTITNDNGK
                     LKAVLDVNGKPFIEATADVGSDLQPAVGGHLNYFGLKEIQKGDSKVIQIIKFPIPYVV
                     QLVKTENPNVKFLMPKKNPLSRLKPIKIKWAAYMQGSFVYPQEQVLKEWESTAKK"
     gene            complement(7567..>8476)
                     /locus_tag="VI935_03155"
     CDS             complement(7567..>8476)
                     /locus_tag="VI935_03155"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLE24629.1"
                     /translation="VSQVIYGNQNWNTAIFGVTPEYIIVRDWSLDLGFFFTKRDFDSA
                     ANVVVLGRTVVENLFLPGNNPLGQIIRIKNIPFRIIGVLSPKGQTTYGSDQDDVVLIP
                     FTTGERKVFGAELSGRVGSIQVSALSKEAIQEATGQIREILRARHRLQPGEEDDFTIR
                     SLEDIAGTAQATTRVLTILLLSVGSISLIVGGVGIMNIMLVSVTERTREIGIRMAVGA
                     KRKDILLQFLIEALVLSVIGGFIGIFIGILGSKILSLSAGWPTLISGGAIVLSFIFAA
                     AVGIFFGFYPANKASKLDPIEALRYE"
BASE COUNT         2560 a         1724 c         1531 g         2661 t
ORIGIN      
        1 gttcacaagg gttaaaccgt ttcctagccc tccgcttact ggtatcgaaa atcgaagaga
       61 agatactcgg taaaaaatct cctgagcagc taaagaggga taaaaatcga aggcagaaga
      121 aacgacgata cagacgggct agaaagaaac ttgcatcaat ctctgcttct aaacaaattt
      181 gaatatcacc ctacttttat tcatttcagt acataattgt aatttgctta atcgcctttc
      241 ataaaaatga agaaaagggg tcgaagaaaa ggtgtcgagt cttttgttga tatattcttt
      301 tatatggttt tcgaaatctc aaaattattt ctaaggtatt atgctcactg ttttttttca
      361 tactatgatt ctcgaatcta cctctaatta gaaattaggc aacaaaagat ttgaccccat
      421 atactcatat actgtattta tcctttacct ccttgtatat gaaatcgaaa tctattagtt
      481 cccttatctt cctcagtgga tgatcctttc ttatcctttt atctagattg atccctatat
      541 aaaacaactt cctctgggga gggtcttggt aactcatcat ggctggtggc ttttatcctc
      601 ctttaagttt ttccccatta tatcaatttt aaaactattt gtaagaggat ttgggcaaca
      661 gcccgttttg ttgatatatt cttttagggt cttgggttca ggcatgcata ttgatttaac
      721 ttatcccata cctcatcgga acatatcaac tcccctaatc tccttagcct ctttgcttcc
      781 tcactgagaa ggggttttag tctctgtcca catggataat caaatatctc ccagcacttc
      841 accaaggctg ttgttacctc accatcgtac acctgctttc ttctccttac ctttaccttc
      901 ttcttatcaa ggttacttag aacccttatc tttctgatta gatacttcct gttcaaacct
      961 gttctcttct cagactcatc cagtagactt ccccttccct tctttgatct agtctttaaa
     1021 tattcaaccc ttatttgctt tagatactga tttcttgagt gcatgtccat cataagaagt
     1081 aagataggct aaaaactgac ttttgaccct atcttccctc tttatttcgg ttactttttt
     1141 atattgcaca accagtatca tttcggttac attttagcct gcagaatacg tcttattgac
     1201 tgaaagagct gtttttaatt ataatcggga ttatagggaa agaggtcttg ttataatacc
     1261 tttttatagg gtgattatag gaacttttgg tcgtataatt tgtagccgga agatttttag
     1321 aaatgccatt atatgacaat gagctacaat taaagtaaac cacacgatat gaaaaaaaaa
     1381 aatgagtctg tgggagagaa ttgaacattg tataaacaca tgggcagata aatcaccccc
     1441 caagtggtgg aaaaagaagt tctcaatagg atcatggtta atattaagat gtattcttct
     1501 tctgcctcta atagtattat catttcgagt tcctagcctt tggtctgtat ggtcattctt
     1561 cacactagtt atcattgcag atattcttgt agtgaatact tcagtatgcc tatttactag
     1621 aaacccccgt agcaaaatca gagctatagt ttttacatta tttgcattta tacaacttgg
     1681 actagccttc tcagtatact atgcaacact tagcacgaag tgtatgctaa atcaatcatt
     1741 gggaatagtt ggaactatct atttcagttt tgtaactatg ctaacattgg gatatggaga
     1801 cgttctacca actcacgagg ttgctcaatt aactacaata atacaagtta acattggtct
     1861 atattttatt tcgattttaa tagcatacgt agttggaaag ttggctcaag ataaatagat
     1921 attgtttctt aacatttgaa aaggacaaaa agtggaagtt ctaggaataa ttccagctcg
     1981 acaaggatct aaaggtatta aatacaagaa tattattccg cttcttggga agcccttgat
     2041 ttcttacact attatggcag caaagaaatc aaagatgatc tcacgtctaa ttgtgtcaag
     2101 cgatgaccct aagatactag caatagctga aagtgaagga gttaagacac cgtttttacg
     2161 tccagaaagt cttgccaccg gcgaaactcc atctgccgat gtagcaattc acgcatttca
     2221 aaaagttcaa tcaatagaag gaaaaaaata cgatgcaatc attctgcttc aacctacctc
     2281 tccattgaga acaacgtacg atattgataa tgcaataaac cttttagaag aaaaagatgc
     2341 agattcagtt attagtgtta tgaaattgga aaaatgccac ccatttaaat tattaagagt
     2401 agaaaatgaa aaattaaaac cactataccc cgaaaaatgg aaagggtcta tacggagaca
     2461 agaaatgcct cctatttata taacaaatgg tgcaatatat tgtattaaga gtcatttatt
     2521 aataaaaaat cgaacattct ggagtgataa taatattcca tatattatgc ctcctgagag
     2581 gtcgattgat attgattcag ttatagattt aaagttggca gaaatccttc taaaggaaag
     2641 gattgaaaat gaataaaata gttttagata atagaactat tggatgcaat gaaccagttc
     2701 taattatagc cgaaatcgga gtgaatcacg gtggatgctt agaaaatgcg ttaaatctta
     2761 tagatgctgc acgagaagct tgtgtagatg cggtgaaatt gcaaagtcac ttagcggatg
     2821 aagaagttat tcttgaaaat tctagtcaaa gtaacaaata tttggaggct tacaaaaggt
     2881 ggaaacggtt cgaattaagc gaatatcaac atagaataat aaaggaatat gctgaggaaa
     2941 agggattaat ctttttttcc actgcgttct ctatggccgc tgttgaaatg ctcaataata
     3001 ttcgagtacc attgttcaag cttgggtctg gggatataac agaccatcaa ttaattgaac
     3061 ttatagcatc aaagggaaaa ccaataataa tatcaacagg aatgagtact gaaaaggaga
     3121 ttgaggatac tgttaacctg atcaggaaat acaatgtacc atatgccatt atgcattgca
     3181 cctcattgta cccttgcccg tatgaatgta tagacttatt ggtgattaag gaactgagag
     3241 caaagtacaa tgtccctgta ggtctgtcag accatactcc aacaatttac actgcagttg
     3301 cgtctacagt tcttggcgta aatattattg aaaaacattt cactttatct aaacggttat
     3361 acggaccaga ccacaaagta tctttaacac cagaagaaat gaaagaatta gtacaaggcg
     3421 ttagggctgt ggaaaaagct ttaaagactt tcaataaggc taactccact gaaatacaag
     3481 aagtaagaga aatgtatcta aaaggcatag ttgcacgaaa tgatcttact aaaggtagca
     3541 caataagaca ggaagatttg atatgcaaga gaggcgcaaa agggataccg gcaaacagaa
     3601 taggtgacat agttggaaaa catgttaagc gtgctatttc aaagaattca tcaatttttc
     3661 ccgaagatat aataggtctt gaataatgac ctgatataaa attaaatttg ccaagagcat
     3721 gcagattata tcaaagaaca aaaatgggag gattattata gatttaggtt attcgttatc
     3781 gataggatta acccctaatt gtctgaatgc ttctcgcact ttattttcat aatcatcctc
     3841 tgtaaatgaa agtagggtca ggcttttgtt attttgttat tatcttcatc tatcccaatc
     3901 tccatcaaaa aactcttact taaagggaag ttggggtcag gcatgcatat tgatttaaca
     3961 ttttagaata ctaaagtaat gtcttcaaac gtaaagacgg tatttttggt gcgtaaaaaa
     4021 agaaaagggg tcggaaaagg ggtcgagtct tttgttgata tattctttta ttcggttttc
     4081 gaaatctcaa aattatttct aaggtattat tatgctcact ggtttttttc atcctatgat
     4141 tctcgaacct acctttaatt agaaattagc caacaaaaga tttgacccca tatgaccctt
     4201 attgagcgca aaacatttca aactagcgca acgctgtcgg caataaccat ctcagaacat
     4261 ccaaagctcc cttaacatta tctggatcta ggggatattt atctcttgga ttcaataaat
     4321 tgagataagc agatggcata gtactaaaat ctccagagtc cttacgaatg taagttttat
     4381 aacaatatcc attcgataca actagtatct gacatgaagg aaacgccttt acatatccgc
     4441 gcgcttcttc cggagcatag tccaatccag aagagaagcc tttcgtttct atgatcaacg
     4501 cacactcatc gtcttttctt agatatgcct ttgagaaaca ggctatatct attttgcctt
     4561 tactaccgga cattttacct ttactactgg gcaactcaat ttttatttgt tgttcagccc
     4621 acccaagagc taacaataag ggcacaatca agaatgttct tgtttcgtgt tctctaatat
     4681 cttcccactt agggtaatgg taataataat ctgccaatag acgaattcgt ctaaaggtat
     4741 tagtgagatc atcagcggca gaaggtctta acccttcact gattaggaat ttaagaattt
     4801 gattatcgtc tatcagttcc gttttacttg gttctagttt ataagcacaa actggaagct
     4861 gaagaataga gtctgctaat tgtttgtgtt tatcctgagg aactttctgt atcgttgcac
     4921 gagtaaggcc gtttgtctga atcggtgtgt ctggaacgcg ccaatctaca tagcaataag
     4981 cttgtaaatc ccagccatca atatccctta accatgattt atctccacaa tctctatgtt
     5041 tcccatctct ttcagctact tcaccgacag caacaatttg atagagtcct ttttttaata
     5101 agacaatgtc tcccttttca acctctttca ttgtctgaga atccccaccg acaaatgcca
     5161 ttccaaattt tataaataga tcagaataat ccctgccaaa tgagccacct gctatctgcc
     5221 agtatttctt actcactcta tccacctcac cttttacatc aacccatttt atgcacaaac
     5281 tacttatatc tcaaaaacat tctaatttca acaagaatat tctttccaat tgcccatttc
     5341 cttggaaacc aagcattttt gcctccacct atagcctgaa attcgagtgc ctctctctcc
     5401 ctcgctgtta tgaaaataat aaaggggacg caaccctttt taaaatattt tagagtaaaa
     5461 gaatggtcgg ggtggtggga cttgaaccca cggcctctgc gtcccgaacg caacgctcta
     5521 gccaactgag ctacaccccg taggcattta atttacaatg cttttggcat gttgaaaagg
     5581 agatgattat tagctgtagg agataaatcg ggagatcctt cagtcgctgt gctccttcag
     5641 gatgacgcca tgcgtcagat tcctcgcagc tccttcgcat tcgctcagga cagggctcgg
     5701 aatgacagga aaaagatcga atttgtatca ctgtgtaaac ccaaaacttt cactctggtt
     5761 gaagtatatt ggtatcccct ttaaacagct ttttaaacct ctccgcatcc gcttttgagc
     5821 cgacgattgt cccccagtgc attgggatta ctgatattgg tttaaattca ttcgcaatct
     5881 ctgctgcttc ttccgctgtc atagtgtatg tgccgccgac cgggagaaga gcaacgtcta
     5941 cgctgagctt tttcatctca ggtattgcgt ctgtatcgcc agcatgataa atcctttttc
     6001 cactgatggt aactataaaa ccaacccaac catttgcctt aggatggtag ctcttaccaa
     6061 tgttgtaagc aggtatagct tggatctcta tatcgccaac tgtaatctta tcgccgggtt
     6121 ttatgatcct tacatcccct tttaggtttg agtctgaatc ctttgtggtt acgatcaccg
     6181 tatctttttt ttgaatatgc cttacatcat cgggggaaag atggtcaaaa tgcgaatgag
     6241 taatcaggat taaatctgct ccttcaccct ttttaacctt ccaagggtca aaatagataa
     6301 tcctttcacc agcttttagc ttgaagctcg catgtccgag ccagcttaga gtaatagaat
     6361 cgtttatttt taacatagct cggctctcca caaattaatc agaagatcct tcggtcactt
     6421 tgctccttcg ggatgacgct atgcgtcaga ttcctcgctg ctccttcgct agcctcttca
     6481 ctgcgttcag agcttcggct caggacatgg ctcggaatga catcatggtg gtgccattcc
     6541 ttatttcttc gctgtagatt cccattcttt gagtacttgt tcctgaggat aaacaaagct
     6601 gccttgcata tatgctgccc actttatttt aatgggcttt aagcgggaaa gaggattttt
     6661 tttaggcatc aaaaatttaa catttggatt ctcagtctta actaattgca ctacatatgg
     6721 tataggaaac tttattatct ggattacttt cgaatcgccc ttttgaattt ctttaagccc
     6781 aaagtaattt agatgacctc cgaccgctgg ctgtaagtcg cttcctacgt cagcagtggc
     6841 ttcaataaag ggtttgccat tcacatcgag taccgcctta agctttccat tgtcatttgt
     6901 tattgttgtg tttccgggct tagcaggaat tccaacttcc tttgtgaatt ttctcataag
     6961 ctcagagcta ttaaagtaac taacaaaata tctacccggt tctccggtag ttgaacccat
     7021 cgtgtatgaa tcctggtctt ttacctgaac agttaggtaa gttagtgtat aggcgccgaa
     7081 tcccgatgtt tgagatggat cgggaactgt atacatattc ataacgacca tattatttgg
     7141 atgtggttcg agtccttgtg gaaggactgc tttaattgcg gctgggtcta cctcaaaccc
     7201 tataattaac atccatgcat ccgtcacaag ctgaggtgcg ggtggaacct tgatatcttc
     7261 ggcagagact gctccgaccg ttcccatgta agcgaatgaa tagataacaa agataaaaac
     7321 catcaggaaa atagcacttg tgttagattt tagcattttc atagatgtct cctccttggt
     7381 taagcttatt tttaggattt atgtttatta ttattccaaa aatacttcag tgtcaataat
     7441 tgttatattt ttacataaaa aacaggtcct cacaatccag tcaaatctca ccatctgaaa
     7501 tgcatatgga gacggccttt aacatatgga gttataatct ctaaactata cattgccagc
     7561 tatctgctat tcatacctca gcgcttcgat tggatcgagt ttggatgcct tgtttgccgg
     7621 atagaatcca aaaaagattc cgacagccgc ggcgaaaata aatgaaagaa caatagctcc
     7681 tcccgaaatc agcgtgggcc aacccgcaga tagggataaa atcttggagc caagaatccc
     7741 aataaaaatc ccaatgaaac cccctatcac actcagaact aaggcctcga ttaaaaactg
     7801 tagaagtatg tcttttcttt ttgcaccgac agccattcta attccaattt ctctggttct
     7861 ttccgtgaca gaaacaagca ttatgttcat aatccccacg ccacctacga tgagagaaat
     7921 ggagccaacg ctgaggagaa gaattgtcaa cacccttgtt gtagcctgtg cagtcccagc
     7981 aatatcttct agggatctaa ttgtgaaatc gtcttcctct ccgggttgga gcctgtggcg
     8041 ggctctcaga atttctctta tttgccctgt ggcttcctga atcgcttctt tactcaaagc
     8101 agaaacctgt atgcttccta cccttccaga aagctctgcg ccaaacacct ttctctctcc
     8161 cgttgtaaat ggaataagca cgacatcatc ctgatcagac ccgtaagtag tttgaccctt
     8221 ggggcttaag accccgataa tcctgaaagg tatgttttta atacgaatta tttgacctag
     8281 tgggttgtta cccgggagaa atagattttc aacaactgtt cttccaagaa ctacgacgtt
     8341 ggctgcactg tcaaaatcac gctttgtaaa aaagaaaccc aagtccagag accaatctcg
     8401 aacaataata tattctggag taactccaaa tattgcggta ttccagttct ggtttccgta
     8461 gattacctgt gaaaca
//
LOCUS       DATDRL010000093         3733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2491728, whole genome shotgun sequence.
ACCESSION   DATDRL010000093 DATDRL010000000
VERSION     DATDRL010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2491728"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VI935_03160"
     CDS             <1..744
                     /locus_tag="VI935_03160"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HLE24630.1"
                     /translation="EFSKEFLDYVTSDVIVVGGGPSGLVAAKDIAKKKYKVLLVERMN
                     YLGGGFWIGGYLMNKVTVRDPGQKVFDEIEVPYKEVSDGLYVADGPYACSKLIAATCE
                     AGVKVANMTMFDDLVYRENGRVAGVVVNWTPISNMPKEITCLDPIALEAKLVIDATGH
                     DAYLISRLAKQGLYNKLPGHGSMWVEKSEDALVEYTGEVAPGLIACGMAVNTAFGLPR
                     MGPTFGGMLLSGKKAAEVAIRRLQEQVRA"
     gene            complement(1482..2330)
                     /locus_tag="VI935_03165"
     CDS             complement(1482..2330)
                     /locus_tag="VI935_03165"
                     /inference="COORDINATES: protein motif:HMM:NF042804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6775 family putative metallopeptidase"
                     /protein_id="HLE24631.1"
                     /translation="MAQIKGVFVYSDYPSISLSIPEIINYLRDHGLSCEDRGNFFEFL
                     DPTRVKALELARRIAGARVLDITSPLDGIHEPTYEEIDLEISRLTGKGSFLGVLYDGF
                     WLQRILYKNMAVKVPHEFRSGFIHVIFTSRLFGTYETSGSLNTLPLDPRPDRGYPRSR
                     RYHARVILTGLPSLISTSGIVEAPARPKEYYWLKAGFIQAGKDLGELDQIYKGRFIEY
                     DDPRLTSVLAPYALKAIFYEINGNPFCDNPSCCLFNSHWQEEVLKAQIGKKLCERHRD
                     TIFRLV"
     gene            complement(2494..2958)
                     /locus_tag="VI935_03170"
     CDS             complement(2494..2958)
                     /locus_tag="VI935_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLE24632.1"
                     /translation="MARVEERLRELGIELNTPPKPQGYYQHAVISGNLIFISGQLPLK
                     GEQLLYKGKVGREVTLEEGVMAARLTAINSLSVMKSELGDLDRIGRIVKLTGYVSSAQ
                     GFNLQAKVINGASELLYDIFGDMGKHARAAVGVYELPLDSPVELEVIAEISP"
     gene            complement(2945..>3733)
                     /locus_tag="VI935_03175"
     CDS             complement(2945..>3733)
                     /locus_tag="VI935_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HLE24633.1"
                     /translation="PGNSGGPLFNLQGQVVGVNTAIIAGGQGIGFAIPIDLAKNIINQ
                     LKDSGKVVRGWLGVLVQQITPEIAEGLKLPEPKGALVSDVTPGGPAAKAGIQRQDIIT
                     QFNGQAVNEMQELPKLVAATPPGTDVQLKYLRNGKEKTARLKVAELPEEIAKSGIPGG
                     EEVEQNLGLVVQEINPQIQRRLRIEDSEGVIITNVDSGGPAQEAGLRAGDLILEINKK
                     QIRNLDDYRKAIDSIKNGETALLLIKRGKNTIYSALKVGGNGKG"
BASE COUNT         1072 a          863 c          761 g         1037 t
ORIGIN      
        1 gagttctcaa aggaattttt agactatgta acaagcgatg tgattgttgt aggtggaggc
       61 cctagcggtc tagttgcagc taaagacatt gcaaagaaaa aatataaagt acttcttgtg
      121 gaaaggatga attacctagg cggtggattc tggatcgggg gatacctcat gaataaagtg
      181 actgtcagag atccaggaca aaaggttttt gatgaaatag aggttcctta taaagaggtt
      241 tccgacggtc tttatgttgc tgacggtcct tatgcttgtt caaaactaat agcagcaaca
      301 tgtgaggcag gggtaaaggt tgcaaatatg actatgtttg atgacttggt ttatcgagaa
      361 aatgggcgcg ttgcaggggt tgttgtaaac tggaccccta tttcaaatat gccaaaggag
      421 atcacgtgcc tcgatcctat tgcacttgag gcaaagctag tcatagatgc tactgggcat
      481 gacgcttacc ttatctctag gcttgctaaa caaggactct ataataaact ccctggacat
      541 ggctccatgt gggtagaaaa atccgaagat gccttagtag aatatacagg tgaagtagcg
      601 cctggtttga ttgcttgtgg aatggctgtg aacactgcat ttggtcttcc aagaatggga
      661 ccgacctttg gcggaatgct tctctcaggc aaaaaggctg cagaggtagc tattcgcagg
      721 ttacaagagc aggttagagc ttagattaat aaagatatac tgtaggggct gggtgacccc
      781 gcccctacta caacagtccg aattccgaaa tccaaaattc cttagaccgc ttcccaatca
      841 cctagttcta cactccacaa aaagacagcg ggagccaagt atcgcatttc caccactccc
      901 gtccacgcgt cgttcagcac aaagcagggt tggatggtgt gcacacccgg accaaataga
      961 acaactgaga tgttggtatt cgtctcaatg tcagttcccg tatgaaagac cacaggacca
     1021 aatacggagc aagcgccccc gtcaatcgaa tatctgatac caagtgtaat gtttacccca
     1081 tcaacggctt tcgcactgaa ctgaaggacg catttggaag agaaaaaatt cttaacagtc
     1141 acgatcgagc ccggaacgag aacatacgac gtcgagctag taaaccggtc atcaaagtca
     1201 cagtcttgct gagaggcggg aatagctcat gctacccccc cactgcaaaa gaaagcccta
     1261 gaacaagggc aaataaaaca gacaaaacac aaatctcttc attttactat cctccctctc
     1321 caaaccaagt taaggtcgag cctttgaggc atttttagca cgaacttgtt cgtgctaaaa
     1381 atagcacaga cgagtctgtg cctacaatat ttgagttgtc gctccaaacc tgtcctgaga
     1441 gcatcgaagg gatggtgctc ctaccaataa ttttatgtgg tttacaccaa cctgaaaatt
     1501 gtatctctat gtctctcaca taattttttt ccaatctgag cctttagaac ctcctcctgc
     1561 cagtgtgaat taaaaagaca acaagatgga ttatcacaga aaggatttcc attgatctca
     1621 taaaaaatag ccttaagggc ataaggggct aaaaccgagg ttaatctagg gtcgtcatac
     1681 tctatgaatc ttcccttata aatttggtca agctcaccga gatccttccc cgcttgtata
     1741 aaaccagctt ttagccaata gtactctttg ggtcttgcag gtgcttccac tattccagaa
     1801 gtcgagatta aagacggaag accggttaga atgaccctag catgatatcg tcgtgatctg
     1861 gggtatcccc gatcaggtcg gggatcaaga gggagggtat tcagggaacc acttgtttcg
     1921 tatgttccaa aaagcctgct tgtaaagatt acgtgaataa acccgctcct gaactcatga
     1981 ggaactttca cagccatgtt tttatagagt attctctgaa gccaaaaacc gtcatacaac
     2041 actccaagaa aactaccctt tcctgtaagc ctgcttattt ctagatcgat ttcctcataa
     2101 gtcggctcat gaattccgtc taggggagaa gtaatgtcta gaacccttgc tcctgcaatt
     2161 cttcttgcta actctaatgc ttttactcta gttggatcta agaattcaaa gaaattccca
     2221 cggtcttcgc aggaaagtcc atggtctctc agataattaa ttatctcagg aattgacaac
     2281 gatattgatg gatagtcgga atagacaaaa acacctttga tctgagccat aacagagata
     2341 gcacaaacat gcgattgtca tttcgagctt agcgaagcaa tccctagatt tttgaaattg
     2401 cttcgttgtg gagtctatcc tgagcaaagc cgaaggactc ctcgcaatga cattaaacct
     2461 tgtttaggta gcaaacttta ttaatgaaac tcactaaggg gaaatctcgg caataacctc
     2521 taattcaacg ggagaatcaa gaggcagctc ataaacacca actgcggcac gggcgtgttt
     2581 acccatatct ccaaatatgt cgtaaagtaa ttctgatgcc ccatttataa ctttagcctg
     2641 caaattaaat ccttgagcac tagagacata accagtcaat tttacaattc ttccgattct
     2701 atctagatcg ccaagctcac tcttcataac agatagagaa ttgatagcag taagtcttgc
     2761 tgccatcacc ccttcttcga gtgtgacttc tctcccaacc ttacctttgt ataaaagctg
     2821 ctcacctttt aaaggaagct ggccggagat aaatataaga ttccctgata taacggcatg
     2881 ctgataataa ccttgtggct ttggaggagt atttaattca atcccaagct ctctcagcct
     2941 ttcctcaacc cttgccattc cctccaacct taagggcaga atagattgta ttcttacctc
     3001 tttttataag taaaagggca gtttcaccat ttttgattga gtcaatagct tttctataat
     3061 catcaaggtt cctgatttgt ttcttattaa tctcgagaat cagatctccc gcacgaagtc
     3121 ccgcttcttg agcaggtccc ccactgtcca cattggttat aataacaccc tcagaatcct
     3181 caattctgag acgcctctgt atttgagggt tgatctcttg gacaacaaga ccaaggttct
     3241 gctcaacttc ctctcctcct ggaatacctg atttagcaat ttcttcaggc agctccgcta
     3301 cctttaatct tgcggtcttt tctttcccgt ttcgtaggta tttcaattgc acatcggttc
     3361 caggcggtgt agctgcaact agttttggta attcctgcat ctcattaact gcttgtccat
     3421 taaattgagt gataatatct tgtctttgga taccggcttt tgcagcaggc ccgccaggag
     3481 tcacatccga aactagagca cccttaggct caggaagttt caaaccctct gcaatctctg
     3541 gggttatctg ctgaactaag acaccaagcc atccgcgtac aacttttcct gaatccttca
     3601 gttggttaat tatgttcttt gcaagatcaa tcgggattgc gaatcctatt ccttgaccac
     3661 cggcaattat agcagtatta acaccaacaa cctgtccttg aagattaaaa agcggacccc
     3721 cactattccc tgg
//
LOCUS       DATDRL010000094         6948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3581158, whole genome shotgun sequence.
ACCESSION   DATDRL010000094 DATDRL010000000
VERSION     DATDRL010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3581158"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VI935_03180"
     CDS             complement(<1..933)
                     /locus_tag="VI935_03180"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLE24634.1"
                     /translation="MKEGSIIEDKDRLEDNIHNIDSSVGSSAVESMTDALTISHTRRI
                     FITMGVMIGMFLAAIEATAVSTAMPTVVSSLGGLSIYSWVFSAYFLTSTITLPLWGKL
                     SDLYGRRIFYIIGITIFLLGSALSGQSNTMTDLIIYRALQGLGGGALLTLGMIIVGEI
                     FSLKERAKVQGLFGGVWGLSSIVGPLIGGFFTDHISWRWVFYLNIPFGLIAALVIGLS
                     LKETHFKAKNIYLDYKGAIILITLITLLLVGLMLIGKENSLSLPAALLMIGVCIPLFW
                     LFISTEKRAKDPILPLDLFSNPFFRTSAATGFLIS"
     gene            1086..1640
                     /locus_tag="VI935_03185"
     CDS             1086..1640
                     /locus_tag="VI935_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLE24635.1"
                     /translation="MESDIELMLRAKTGDDSAFTELMRRHYKGVVNYVYRFTNDRANS
                     EDLAQEVFLRVYRSVKRYKPQAKFSTWLYKIATNLCITEVKSRNKEQSVSLDEMQEKA
                     GELGDSKSEGPSDLTLRREIGTAIFEALKSLPERERVAIILCKYEDLPYEEVAEVIGC
                     TIGAVKAYVHRGRMKLIEKLKTYL"
     gene            1655..2143
                     /locus_tag="VI935_03190"
     CDS             1655..2143
                     /locus_tag="VI935_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HLE24636.1"
                     /translation="MECEKIKELILHYSEGDLNQSDKLGVEKHLKECKECNIYFEQSH
                     KVWNLLDNWSAVEIKNDFLPRFWDRVITEDRKKGNVFDFFRNLRVNLISGGVGVIILI
                     LVVFLVNILVSHHENIVFTEEDRSDEELLIEFDRSITKETAESLEIYGPWSDEIEENK
                     GG"
     gene            2147..2635
                     /locus_tag="VI935_03195"
     CDS             2147..2635
                     /locus_tag="VI935_03195"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HLE24637.1"
                     /translation="MKRKIKKDWILTPGFHHMKFFILKTVSVSLFFMLAFGTFCLNTY
                     GYDGDSPGKWWKNKKIVKQLELSNEQMNRIEGIFSSNKGRIKELDSDLKKKEREFSDT
                     VKNPNSSREEILRMNNEVEEVKGKLRKAYIDMRLQIRDVLTPEQRIKLQEIKTKHGKP
                     YP"
     gene            3169..5214
                     /locus_tag="VI935_03200"
     CDS             3169..5214
                     /locus_tag="VI935_03200"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HLE24638.1"
                     /translation="MKTSHALTIFTHLMALTGFISVSITGEVDGLSIVVFTVSLILSF
                     FNEWSGKRYYLSNRVLSVLAIILLIFVLANTTLFGMEIFSGILVFLIYTQVLKLLGLK
                     ELRDIVQIYILSFFQFLAGTILTVSFSYGIVFVVYVALSIWAIITFNMRRESIEARSY
                     DDPKVVTPLFLGITGVASFGIFFFTAFIFVSVPRLGSGFFTSNFLKPAELSTGFSDQV
                     KLGQVGKIKLDTTPVMRIKILDRDPETLPKPIYWRGIALDEFDGKEWRVGDLNQNVYK
                     KNKEGIILVNETNNKTISQEIVTEPLDTDILFAANLPVGFWGVAGGKIEEVNDSYIIP
                     GQVSYRLKYLAYSDLSIPSAEELRSEKEDYPNYIKNLYLKLPPLSKRVQELAQNIAST
                     DGNSYDKAISIKRYLINNMNYTITLEKGASEFPLDDFLFDKKAGHCEYFATAMVVLLR
                     EVGIPARIVNGFLDGEWNKYGRFFLVRQSDAHSWVEVFFRNYGWVLFDPTPASDEGFP
                     KGNFIANYLDYLKYRWSRYIVDFSQRDQINLLSGLQSRWRWQKSKFQNRLEINYKFNK
                     KWLLGFVILALLMWTVLLNKKFKLLKIYESKKPEEKASIIYKNALKLLSKKGLIKPDF
                     VTPREFARDLVYENGIDFQILNDLTEKYLNLRFGIEEEKLGFQELEKLLYRLKNEIK"
     gene            complement(5278..5781)
                     /locus_tag="VI935_03205"
     CDS             complement(5278..5781)
                     /locus_tag="VI935_03205"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HLE24639.1"
                     /translation="MNLVNKMIRAAKLDAQLYEEVEKDIGATKEAFLVVLIGAICNGI
                     GSVGMLGAKGIITGLISGIIGWLLWSAVVYLIGVKIFKHTSSMGELLRCLGYAYSPYV
                     LSILGIIPGIGIIIRLIASIWVLVAFVIAARQALDCGTGRAILISVLGFIAFLIVFFL
                     FSLLLRT"
     gene            complement(5919..>6948)
                     /locus_tag="VI935_03210"
     CDS             complement(5919..>6948)
                     /locus_tag="VI935_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLE24640.1"
                     /translation="ADYYSARGYVEVAEINENDVLYTCLPLFHSNAQVLCVYPALLAG
                     AMVTIHERFSASQFWKQIKDSKATVFNSLGAMSYFLWRQPSVSEEKEHSVRLAIITPA
                     PHDMLEEFMERFNLKILEGYGLTETGVVTYMRPHEPFRVGSCGKEAPGYEVKIADPES
                     DEELDSGQVGEIIVRPRIPNIMLYQYHKMPEKTVSDFRNFWFHTGDAGKMDKDGYVYF
                     VDRVKDYIRRRGENISSFEVEKIVCQHPAVAEAGAIGIKSGEGKFAEDDLMIVVVKKS
                     DKNVTYEELIKHCEERMPYFMIPRYIRFVESLPKTPTERVQKHILKEEGITTDTWDME
                     KVGYKIKR"
BASE COUNT         2159 a         1253 c         1415 g         2121 t
ORIGIN      
        1 tgatataagg aatccagttg cggcagaggt tctgaaaaat ggattagaga ataaatcaag
       61 cggaagaatc ggatccttag ccctcttttc agtactgata aatagccaaa aaagaggaat
      121 gcaaacgccg atcataagaa gcgctgcagg caaactcagg ctattttctt taccaatcaa
      181 cattagccca acaagtagta gtgtaattag ggttataaga ataatagcgc ctttataatc
      241 aagatatata ttctttgctt taaaatgagt ttctttaagt gaaaggccta ttactaatgc
      301 cgcaataaga ccgaatggta tgttaaggta aaatacccat ctccaagata tatgatctgt
      361 aaaaaatccc cctatcagtg gacctacaat gcttgatagc ccccacacac ctccaaaaag
      421 accctgtacc ttcgcccttt ccttgaggga gaaaatctca cctacaatta tcattccaag
      481 tgtaagaagt gcgccacctc ctagcccttg aagcgctcga tagataatca ggtctgtcat
      541 tgtgttagat tgacctgaaa gagcggaacc caaaagaaaa atcgtaatac cgattatgta
      601 gaagatcctt cttccatata ggtcagatag cttcccccat aaaggaaggg ttattgtaga
      661 ggtcagaaaa taggctgaga atacccagct atatatgctg agtcctccga ggcttgacac
      721 gactgtaggc attgccgtac tcacagccgt agcctcgatg gcggcaagga acattccaat
      781 cattaccccc atggttatga aaatccttct agtatgagaa attgttaaag catctgtcat
      841 tgactcaacc gcactcgaac caactgatga gtctatgtta tggatgttgt cttcaagcct
      901 gtctttatct tctattatgg atccttcttt cattctgtct tcattgattt agagcattag
      961 cttaccatag tttcattatc aatgagtttg acttatggac tataatagct gaaacttttt
     1021 tttaaactct tagttattat ttgtaaacct gcggtatgca gacgttcgtg agcctcagtc
     1081 gaacgatgga aagcgacatt gagcttatgc ttagggctaa gacgggggat gattctgctt
     1141 ttactgagct tatgaggagg cattataaag gggttgtgaa ttacgtatac aggtttacaa
     1201 atgatagggc aaactctgag gatttagcac aagaggtttt tcttagggtt taccgttcgg
     1261 taaaacgata caaacctcag gcaaagtttt ctacttggct ttataagatt gccactaacc
     1321 tttgcattac ggaggtaaag tctaggaata aggaacaaag tgtaagttta gatgagatgc
     1381 aggaaaaggc cggagaatta ggagattcaa aatcggaggg cccttccgat cttaccttaa
     1441 gacgtgaaat cggaactgcc attttcgagg ctttaaaatc gttgcctgaa agggaaagag
     1501 tagctattat cctttgcaag tatgaggatc tgccatacga ggaggttgca gaggtaattg
     1561 gatgcacaat tggagcagta aaggcttatg ttcatagagg aaggatgaaa ttgatagaaa
     1621 agctgaaaac ctatttataa agaggggaag taccatggaa tgtgaaaaga taaaggagtt
     1681 aattctccat tattcagaag gagatttaaa tcaaagtgat aagctagggg ttgaaaagca
     1741 tttaaaagaa tgcaaggagt gtaatatcta ttttgagcag tctcataagg tgtggaatct
     1801 gctagataac tggtctgccg ttgagataaa gaatgatttt ctcccgagat tttgggatag
     1861 ggttattaca gaagatcgaa agaaaggcaa tgtcttcgat ttttttagaa atttgagagt
     1921 caatttaata tctggagggg ttggggttat aatacttatc ttagtagttt tccttgttaa
     1981 tattcttgtt tctcatcatg aaaatattgt ttttactgaa gaagacagat cggatgaaga
     2041 gcttttaatc gagtttgaca ggtccattac aaaggagact gcggagtcac tcgagattta
     2101 tggaccttgg agtgatgaaa ttgaagaaaa taagggagga tagaacttga aaagaaaaat
     2161 aaaaaaagat tggattctaa cccctggatt tcatcatatg aaatttttca ttttaaaaac
     2221 agtatcagtt tcattattct tcatgctagc ttttggtacg ttctgtttga atacatatgg
     2281 ttatgacggc gatagtccag gcaaatggtg gaagaataaa aaaatagtaa agcaattgga
     2341 gcttagtaac gaacagatga atcgaataga gggaattttt tcttctaaca aaggacggat
     2401 aaaggagctt gattctgatc ttaagaaaaa agagagggag ttcagcgata cagttaaaaa
     2461 tccaaattcg agtcgagaag agatcttgag aatgaataat gaagtggaag aagtcaaagg
     2521 aaaattgaga aaggcttata tagatatgcg tcttcaaata agagatgttt tgacaccgga
     2581 gcagagaata aagcttcagg aaattaagac aaaacatgga aaaccatatc catgataaat
     2641 caagtgctta attaggtata ttaatctttt aaagtaaact tttagcttgt aattccccgt
     2701 agcttgtatg gggtttccac aatagtgtac tgtcattgcg aacgaagtga agcaatctct
     2761 cattttagat tgcttcgtcg cgtagtttat cctgagctag acgaagggct cctcgtaatg
     2821 acagccataa cggtatattt ttaagaccat tctctgcagc ttggaggctg tctccgacaa
     2881 ttatggatgg taaaaatttg acccctcaga atgccccagg atcaacgatc ggcaatctgg
     2941 gaggggtttt gctaccccca agaatgccta tttttgcatt tatcggacag cctgcttgct
     3001 atggggttct tcatttccaa aggttattct ttcgattacc ttaactagcg tctgttcatc
     3061 taagaatgcc tttttgccga atatatggga aaatattaaa atattgtgtt cgcatttatt
     3121 ggtatttggc ttaaaggcct tttgggttta ataggtgctt gagtttaaat gaagacgtct
     3181 catgccctca caatctttac tcatcttatg gccctcacgg gatttatatc cgtttcaatc
     3241 acaggagagg tggatgggct aagcattgtt gtctttactg tctcgcttat attaagtttt
     3301 tttaatgaat ggtcagggaa aagatactac cttagcaata gggttttaag tgttctcgct
     3361 attatacttt taatctttgt acttgcaaac acaacccttt ttggtatgga gatttttagt
     3421 ggcatattgg ttttcttgat atatacacag gttttaaaac tactaggatt aaaggagtta
     3481 agagatattg ttcagattta tatactgagc ttttttcaat ttcttgctgg aaccattctc
     3541 accgttagtt tttcctatgg aatagttttt gtcgtttatg ttgccctatc aatttgggca
     3601 atcataacct ttaatatgag aagggaatcc attgaagccc gaagctatga cgaccctaaa
     3661 gtagtcaccc ctcttttcct agggattaca ggagttgcta gctttggaat attctttttt
     3721 acagctttta tatttgtgtc agttcctagg ttagggagcg gattttttac ctcgaatttt
     3781 cttaaacctg cagagttaag cactggtttc tctgaccagg taaaactggg acaggtaggg
     3841 aaaattaaac ttgataccac acctgttatg aggataaaaa ttttagatag agatcccgag
     3901 actctcccga aaccgattta ctggcgtggt atagcgcttg acgagtttga cggaaaagaa
     3961 tggagggtag gtgacttaaa ccagaatgtt tataaaaaga ataaagaggg gattatactg
     4021 gtaaatgaaa caaacaataa aactatatca caggagatag taactgagcc acttgatacg
     4081 gatatactct ttgcagctaa tctcccagtg ggattttggg gagtagcggg tgggaagata
     4141 gaagaggtga atgattcata catcattcct gggcaggttt cctatagatt aaagtactta
     4201 gcctattctg atttgagtat tccttctgct gaggagttaa ggagtgagaa agaagactat
     4261 ccgaattata taaaaaatct ttatctaaaa ctccccccgc taagcaaaag agtccaggag
     4321 cttgcacaaa atattgcatc tactgatgga aatagctatg acaaagctat ctcaattaaa
     4381 aggtatctaa tcaataatat gaactacacc ataacccttg aaaaaggggc gtcagaattt
     4441 ccactggatg attttctctt tgataagaaa gctgggcact gcgagtattt tgcaacggcg
     4501 atggttgttc tcttacgaga ggtaggtatt cctgcaagaa ttgtgaatgg atttcttgat
     4561 ggagagtgga ataagtatgg aaggtttttc ttagtgcgtc aaagcgacgc ccactcctgg
     4621 gttgaggtat tcttccgtaa ttacggatgg gttcttttcg atcctacccc ggcaagcgat
     4681 gaaggattcc ctaaagggaa ttttattgct aattatctcg attacttaaa atacagatgg
     4741 agtaggtata tcgttgactt cagtcagagg gatcaaatta atcttctctc cggtcttcaa
     4801 agtaggtgga gatggcaaaa aagtaagttt caaaacaggt tagaaattaa ttataaattt
     4861 aataaaaaat ggctccttgg atttgtaatt ttagctttat taatgtggac agttttgtta
     4921 aataagaaat ttaagcttct taagatatat gaaagtaaaa aacccgaaga gaaggcatcg
     4981 atcatctata aaaacgccct taaactcctt tctaaaaagg gtcttataaa gccagatttt
     5041 gttaccccaa gagagtttgc aagggatctg gtttatgaga atggaattga tttccaaata
     5101 cttaacgact tgacagaaaa gtatctgaat ttaagattcg ggatagaaga agaaaagtta
     5161 ggatttcaag aattagagaa actcctttat agattaaaga atgagattaa ataaatattt
     5221 ataaatagaa cagcgtgaaa aaattaacct ttattagtct gcctccaatc aataatttca
     5281 agtacgaaga agaagagaga aaaggaaaaa aacaatcaga aatgctataa aacctagaac
     5341 tgaaatcaaa atcgctcgac ccgtgccgca gtcgagtgct tgccgtgcgg caataacaaa
     5401 agctacaaga acccaaatag atgcaattag acgtattata ataccaattc cgggaataat
     5461 tccgagaata cttaggacat atggggagta ggcataaccg aggcatctaa gaagctcacc
     5521 catgctggat gtgtgtttaa aaatttttac ccctataagg taaacgacgg cactccatag
     5581 aagccaacca ataattcccg atataagacc tgttattatt ccctttgccc ctaacattcc
     5641 tacgcttcca atcccgttgc agatggcacc tatcaatacc accaaaaagg cttccttcgt
     5701 tgctcctatg tccttctcta cttcctcgta gagctgagca tcgagcttag ctgcccttat
     5761 cattttatta actagattca ttcccaatcc tcctttaagt tattgtttta tttatatcag
     5821 ttttctagca taaaagtcaa cttattgtag cacaacggaa gctttcttaa aaattcaaga
     5881 caagttctac atgtgcgtta gagttgcttg attgatgact atcttttgat tttatatcca
     5941 accttctcca tatcccatgt atccgtcgtt ataccctctt ccttaagaat gtgtttttga
     6001 accctttcag tcggtgtttt agggagtgac tcaacaaacc ttatatacct cggaatcata
     6061 aagtatggca ttctttcttc acaatgtttg atgagttcct catatgttac attcttatct
     6121 gattttttaa ccactactat catcaaatcg tcctcagcga attttccctc tccggactta
     6181 ataccgattg ctcctgcttc tgccactgcc ggatgttgac atacgatttt ctcaacctca
     6241 aatgaactta tattctcgcc tctccttctt atgtagtctt taaccctgtc gacgaaatag
     6301 acgtatccgt ccttatccat ttttcctgca tctccagtat gaaaccagaa gtttctaaag
     6361 tcacttactg ttttttctgg catcttatga tattggtaaa gcattatgtt tggaattctg
     6421 ggtctaacta tgatttcgcc gacttgaccg ctatcaagtt cctcatcact ttcaggatct
     6481 gcaattttta cctcataccc aggtgcctcc tttccacatg agcctactct aaaaggctca
     6541 tgtggacgca tatatgtgac tacacctgtt tctgttaatc cataaccttc aagaattttt
     6601 agattgaatc tttccataaa ttcctcaagc atatcatgcg gagccggagt tattatagca
     6661 agtctcacgc tatgttcttt ttcttcagaa accgaaggct gacgccagag aaagtagctc
     6721 atagcgccaa gggagttaaa aaccgttgcc tttgaatcct ttatttgttt ccagaactga
     6781 gaagcgctga atctttcatg aatggtgacc attgccccgg ctaagagggc ggggtaaaca
     6841 catagcactt gggcattaga gtgaaaaagg ggcaaacagg tataaagaac atcattttcg
     6901 tttatttcag ctacctctac gtatcctctg gcgctgtaat aatcggcg
//
LOCUS       DATDRL010000095         5811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2332074, whole genome shotgun sequence.
ACCESSION   DATDRL010000095 DATDRL010000000
VERSION     DATDRL010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2332074"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            515..1372
                     /gene="htpX"
                     /locus_tag="VI935_03215"
     CDS             515..1372
                     /gene="htpX"
                     /locus_tag="VI935_03215"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005986132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HLE24641.1"
                     /translation="MSTSLKTAFLLVILTLVFVYVGRLIGGTTGMTIAFIIALVMNLM
                     SYWFSDKIVLAMYRAREVTPEEAPQLHNMVRNVAMQAQIPMPRVYIIPTQAPNAFATG
                     RNPENAAVAVTEGILRLLTPQELEGVLAHEISHVKNRDILIGSIAATLAGAIGYLAHM
                     AQFALIFGGYGRSSDDEGRGSLIGSLAMIILAPIIALLIQLAISRAREYKADESGAHI
                     TRRPLELASALKKISSGTERIPLDANPGTAHLFIMNPLRGGLAGLFSTHPPVEERIGR
                     LEKLSREIV"
     gene            complement(1475..1768)
                     /locus_tag="VI935_03220"
     CDS             complement(1475..1768)
                     /locus_tag="VI935_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HLE24642.1"
                     /translation="MERAVVIINEAPSSMRTWNGFRLAGALIGADMKVEVFLLNDGVF
                     CALKGQNPPDEIAGQNTGVKIKEILGLGARITLCTQCAQTRGITEGMVVEGVE"
     gene            2096..3361
                     /locus_tag="VI935_03225"
     CDS             2096..3361
                     /locus_tag="VI935_03225"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24643.1"
                     /translation="MVKTSISITRKLLGDLLLEAGVITEDQLREAHERQWSQGGRILS
                     LLVELGFITETKLKDFLTKKLKLPLLNPLTSQIEEDTLKLITAETAYKLKVIPVRLEK
                     NLGRKILILATSDPDIEIADAVSFITGLDVKLGYASEMDIETALKQYYRNGKSVSNDK
                     RPNKISEKVIYNTPLEECEKSQQQLINSLNLLLEDTKRSIGDFGLPSWCNSYTLPTPA
                     HEFELRGDIQNIEKEINSIKDKISKHKDLTWKLEQRKILFTGTGKEIEDQVKEILEEM
                     GFTFMEGLQDGEGLIFKYNDRITVVEIKGVPGSALEKHAIQLEKGIIDYYSSHEVMPK
                     GVLVVNVYKDTPLDDRNGAPFPSNLISYSEKRDHCLITGLQLLGLYLDCKNNPDKKAQ
                     VIASIFSTSGVFGYYQDWRGFLKLEESIR"
     gene            3492..4424
                     /locus_tag="VI935_03230"
     CDS             3492..4424
                     /locus_tag="VI935_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014041755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLE24644.1"
                     /translation="MIQVQNLTKRYGNLVAIDNVSFSVGQGEILGFLGPNGAGKTTTM
                     RIISGYMPPTDGTVCVGDFDVLEDPIKAKRQIGYLPENPPLYKDMTVQGYLDFVADIK
                     YVSGKNKKDKIDIAMERCGITDVRKRLIGNLSKGYRQRIGIAQAIVHDPAVLILDEPT
                     IGLDPKQIIEIRYLIKSLAGDRTVILSTHLLPEVTMTCTRVIIINEGKIVLQESLETL
                     SQRINGAQILFLKLNYNDRGNDVNEKILSIPTISEVKESAYGEFIIQTKGGADIREEL
                     VKMIVQNGWGLLELRPLTHALEEVFLRVISTEGE"
     gene            4425..5177
                     /locus_tag="VI935_03235"
     CDS             4425..5177
                     /locus_tag="VI935_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLE24645.1"
                     /translation="MKPVYTILRREFKSYFASPIAYIVLFVFLILSGIFFFLYLQSFV
                     ESQFDPRFQFFRERLNLNDFVIRPYFGTVSIILLLMIPLITMRLIAEEKKNFTAELIF
                     TSPLRIIHIVLGKFLASLSFFGVMVVLSALYVVVLKAYGNPDAGPVLSGYLGLFLLGG
                     CFIAVGLFASSLTENQIIAAVISFGTLLLFWIIGASSGDEGSILGYISIINHLDNFTK
                     GVIELKDVVYYLSLIFFGLLLTHVTLESERWR"
     gene            5174..>5811
                     /locus_tag="VI935_03240"
     CDS             5174..>5811
                     /locus_tag="VI935_03240"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HLE24646.1"
                     /translation="MKRKRFLLHGTNAFVFPIVALGILIIINYLAYKRDARFDVTEGK
                     LYSLSDQTVKVLNNLDKEIEVLAFFKEVGVDRKEFQDLINEYKRKSDKIKVQFVDPVK
                     EPGLSKKYEVNEYGTVVLASGGQSVKIKLEDLISGGIVDNSEEEITNALIRLSQAIKK
                     TIFFLAGHGERDIQNETEPEGYGSLKHAIEGESYEVKDFVLLTDKSIPKENS"
BASE COUNT         1850 a          993 c         1188 g         1780 t
ORIGIN      
        1 gatcaatatc tgaattttaa ggagattagt tctggtgaaa ctaaaaagat taaaactcat
       61 actctacccc ttgagaatct acttacaggt gacattttta ctttgcatca ggggtgacat
      121 attcactttg tattgacaga attcttatta ataattatta atttgcaaac aagacagcgg
      181 caacccctta atttcggtat taaacttata tcatccttaa tattagcgta aataaacctc
      241 atatcgaaga ctatattcat aacccacact tacttcactt atggaactga ggtcaggcgt
      301 ttaccaaaca aatagagaat agtgatttat ggtacaggtc acgaaaccat ttatgtatta
      361 tttatgatca gaaaacccat cgaattttaa attatacttt atgtttatcc ctgtagaact
      421 agcgaaaaaa aacagtgaaa aacgttgaca atcagggaat aatgacttaa attaatattg
      481 tttagctttt gtttgtgaaa taggaggcgc agatatgtct acttcattaa agaccgcatt
      541 tttattggtg attttaactc tagtattcgt atatgttgga aggctgattg gcggaacaac
      601 aggtatgact atagctttca taatagccct tgtgatgaac ctcatgtcct actggttctc
      661 tgataagatc gtgcttgcta tgtaccgcgc aagggaggtt acgcctgaag aggctcccca
      721 gcttcataat atggtaagaa acgtagccat gcaggctcag atcccgatgc caagggttta
      781 tataatacca acacaggcac caaatgcctt tgcaacagga agaaatcccg agaacgctgc
      841 tgtagctgtt accgaaggca ttcttagact acttacacct caggaacttg agggtgtatt
      901 agcacacgag attagccatg ttaaaaatag ggatatcctg attggttcga tagcagcaac
      961 gcttgcaggt gctatagggt atttggctca tatggcccag ttcgcactta tttttggagg
     1021 gtatggaaga agcagtgacg atgaaggacg tggcagtttg ataggctctc ttgctatgat
     1081 aatattggcg ccaataatcg ccctacttat tcaacttgcc atatcacgtg cgagagagta
     1141 taaagccgat gaatccggtg ctcatataac tagaagacct cttgaactag caagtgctct
     1201 taaaaagata agttccggca ccgaaagaat tccacttgat gcaaaccctg gcacagcaca
     1261 tctatttata atgaatccac ttcgtggtgg tttagctgga cttttcagca cacatccccc
     1321 ggtagaggaa agaataggaa gacttgaaaa gctttcaaga gagattgtat aaaataattt
     1381 aggggcgacc gggtggtcgt ctataccaca ttatctagaa actcactacc ttatttgatt
     1441 cccttataga tttagcgaga tcaaccatgc taactcactc cactccttca actaccatac
     1501 cttcagtgat gcctctagtc tgagcacact gggtgcaaag cgttattcgt gctccaagtc
     1561 caagaatctc ttttatcttt acccccgtgt tttggcctgc aatttcatca ggcgggtttt
     1621 gtcctttcag tgcacagaaa acgccgtcat ttaggaggaa aacctctacc ttcatatcag
     1681 cgcctataag ggcgcctgcc agcctaaaac cattccaagt cctcatactg cttggggcct
     1741 cgtttattat tacaacggct ctttccatct gagttactcc tcaatggttg tttaagcagc
     1801 tcaattcaga gtaatcacaa aaatgtacat aactatgtga tattttattt ttgtaaggag
     1861 ttaattgcaa taactaagga ataataatta gggtatttag taattttatt ctaaaatctt
     1921 tacaataaaa acttattttt gtttaaagat ttaaatagct tttttaaatc gtagtgtgat
     1981 aaaccttgat ataaaagtgg tttgcgtatc aaataaagtt gtactattat gttaatttat
     2041 ctaaactaca gcagagtagc ccgtttatca taaataggct cctagagaat atatcgtggt
     2101 aaagacatcc atatcgataa cgaggaaact cctaggtgac cttttactcg aggctggtgt
     2161 aattacagaa gaccaactac gggaagctca cgagcgtcag tggtcacaag gtggaagaat
     2221 attaagttta ctggtcgaac ttggatttat cactgagact aaactcaaag attttcttac
     2281 aaagaagctt aagctaccgt tacttaatcc gctgacctct caaattgaag aggatacatt
     2341 aaaattaatt acagctgaaa ctgcttacaa actcaaggta atacctgtaa ggttagaaaa
     2401 gaatttagga agaaagattt taatactggc aacttccgac cctgatatag agatagctga
     2461 tgcagtttca tttatcactg gtttagatgt taagttaggg tacgcttctg aaatggatat
     2521 tgaaacagca ttaaagcagt actatcgtaa tggaaagagt gtttcaaatg ataagaggcc
     2581 taataagata agtgagaaag taatttataa tacaccccta gaggaatgtg agaaatcgca
     2641 gcagcaacta atcaattcgc taaacctatt attggaggac acgaaaaggt ctatcggtga
     2701 tttcggactg ccatcatggt gtaatagcta tacactacct acaccagctc atgaatttga
     2761 gcttaggggc gatatacaaa acatcgaaaa agaaataaat agtatcaaag ataaaataag
     2821 taaacataaa gatttaactt ggaagttgga acaacgtaag attttattta ctggcacagg
     2881 caaggagata gaagatcaag tcaaggaaat attagaggag atgggattta cttttatgga
     2941 ggggttacaa gatggggagg gtttaatatt caaatataac gacagaataa cagtagttga
     3001 gattaagggt gtaccaggga gcgcattaga aaaacacgct atacagttag aaaaaggtat
     3061 tattgactac tactcatctc atgaagttat gcctaagggt gttttagttg ttaacgttta
     3121 taaggacaca cctctagatg atcgaaatgg ggctcccttt ccaagtaatt tgataagcta
     3181 ctctgaaaaa agggatcatt gtctaataac tggcttacaa ttattaggtt tatacttaga
     3241 ctgcaagaat aatccggata agaaagctca ggtgatcgcc agtatatttt ctactagtgg
     3301 ggtctttggt tattatcaag actggagagg ctttttaaaa ttagaggaaa gtatcagata
     3361 aaagtttgct agtaatatta atattgtatt taacctaaac tggatagtca tattagttac
     3421 tgtttcctct tgacatcact tctatgttgg ttaattttga acttaagttt ttgtaaaact
     3481 atttttcgga tatgattcaa gttcaaaatc ttacaaaacg ctatggcaat ttagttgcta
     3541 tagataatgt atccttcagc gttggtcagg gagagattct tggttttctc ggtccaaacg
     3601 gtgcaggaaa aaccacaaca atgcggataa ttagtggata catgcctccc actgatggaa
     3661 ctgtctgtgt gggcgatttt gacgttttag aagatcccat taaagcaaag aggcaaatag
     3721 gttatctacc ggagaatccc cctttatata aagacatgac tgttcagggt tatctcgact
     3781 ttgttgctga tatcaaatat gtgtccggaa aaaataaaaa agacaagatt gacatcgcta
     3841 tggaaaggtg tggaattaca gatgtgagaa aacgtctaat cgggaatctt tctaaaggtt
     3901 atagacaacg catcggaatt gcgcaggcaa ttgttcatga tcctgccgtt ctaatacttg
     3961 atgaacctac aataggactt gacccaaagc agattattga gattcggtat cttattaaaa
     4021 gcctagcggg ggatcgtacc gttatcctga gtactcacct acttccagag gtcaccatga
     4081 catgtacgag ggttataatt ataaacgaag gaaagattgt cttacaagaa tcccttgaga
     4141 cactttcaca aaggataaat ggtgctcaga ttttattcct caagcttaac tataatgata
     4201 gaggtaatga cgttaatgaa aaaatcctat ccatacccac aatatcagag gttaaggaaa
     4261 gcgcttatgg agagtttatc attcagacaa agggaggagc tgatattaga gaagaactgg
     4321 tgaagatgat tgttcaaaat ggctgggggt tgttagaact ccgacctctt acgcatgcac
     4381 ttgaagaggt atttcttaga gttatatcaa cggaaggtga gtagttgaaa cccgtttata
     4441 ccatcttaag aagggaattc aaatcgtatt ttgcttctcc tatagcatat attgttttat
     4501 ttgtatttct tattctttct ggaatcttct tctttttata tctccagagc tttgtcgaat
     4561 ctcagttcga cccaaggttt cagttctttc gagagagact taacctaaat gattttgtga
     4621 ttcgaccata ctttggtaca gtaagcataa tactacttct catgatacct cttatcacga
     4681 tgaggcttat cgcagaggag aaaaaaaact ttacagcgga acttattttt acttcaccgc
     4741 ttagaataat acatatagtc ttaggtaagt ttctcgcttc tctctctttc tttggagtaa
     4801 tggtagtact ctctgcttta tatgttgttg ttcttaaggc gtatggaaac cccgacgcgg
     4861 gtcctgtgct ttcagggtat ctaggtcttt ttcttctagg tggttgtttt attgctgttg
     4921 ggctttttgc ttcttctttg actgagaatc aaataattgc cgccgtgatt tcttttggaa
     4981 cactgcttct tttttggata ataggggctt catctggcga tgaaggttct atcttagggt
     5041 acatctcgat aattaaccac cttgataatt ttacaaaagg tgtgattgag ctaaaagacg
     5101 tcgtttatta tctttctcta atcttctttg gactccttct aacccatgta acacttgaat
     5161 ctgagaggtg gagatgaaga gaaagcgttt tcttctccat ggaactaatg cctttgtatt
     5221 tccgatagta gcgcttggga ttctaataat aataaattat ttagcctata aacgtgatgc
     5281 cagattcgat gttactgagg gaaaattata ttccctttca gatcagacgg ttaaagtgtt
     5341 gaataatcta gataaagaga tagaggtttt agcctttttt aaggaggttg gtgttgatag
     5401 aaaagagttt caggatttaa taaatgaata taaaagaaaa tcggacaaga taaaggtgca
     5461 attcgttgac cctgttaaag aaccagggct ttcaaagaag tatgaggtta atgagtatgg
     5521 gactgtagtg cttgcaagcg gtggtcaaag tgtaaaaata aagttagaag acttgatatc
     5581 gggaggtatt gtagataata gtgaggaaga gattacaaac gcattaataa gattatcaca
     5641 ggcgataaag aaaactattt ttttccttgc tggacatggg gaaagggata ttcagaacga
     5701 aacagaaccg gagggatatg gaagtttaaa gcatgccata gaaggagagt cttatgaggt
     5761 taaagatttt gtacttctaa cggataaaag tattcctaaa gagaattcaa t
//
LOCUS       DATDRL010000096         9489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2749064, whole genome shotgun sequence.
ACCESSION   DATDRL010000096 DATDRL010000000
VERSION     DATDRL010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9489
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2749064"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..1482)
                     /locus_tag="VI935_03245"
     CDS             complement(316..1482)
                     /locus_tag="VI935_03245"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HLE24647.1"
                     /translation="MSTNTSLGYTNVRSENPTWKYRLFWYLAYGYFQNEHFFNSFVAK
                     LKVSLSKVEDRFYRKKWEDIELRKPIFIIGPHRSGTTILQQMLSLHSTVATPRTYSDM
                     FDLIPILAKQYLRPLIRSRAYRRIDRIIVGFDTPQEAQGLISRYFDKKQVLYNPTTPE
                     DIRNYMRKLLYLEGKTRFLWKVPYLTIQIPEVLSLFPDARFIYLYRDPVACVNSKLKF
                     IKVWQEMAQSPSLLYHRLVGRNQHFEQSGMGYFMEQANRTVNLKPTSPDPLAMAMDHL
                     QWIEQALRDLGKLGSSNTRCFLDYSALILEPHTSLRHLFEFLGLPDESEAILAKLEEL
                     GMPLTMPESKLSYIPEESLPAITGVCRERMKQCCSAMDWKGWQVIGPIPTDSHS"
     gene            complement(1505..2902)
                     /locus_tag="VI935_03250"
     CDS             complement(1505..2902)
                     /locus_tag="VI935_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HLE24648.1"
                     /translation="MTTASFPPGSKGIPLIGDMLAFYREPHSFLLNLAREYGDIAHFR
                     IGPLNAFLINHPDYIQDVLVNNYQNFAKGRILQRLKFLGGEGLITTSDHHYHRRQRRL
                     IQPAFHRQRIAAYGAVMMEYGARIRERWQDGATLDIAQEMMRLTLAIVGKALFDADIE
                     SEAKEIGEAWTIIMERAPMLLVPFSELLDRFPLPSNRRFRKAKDRLDDTIYRMINERR
                     ASGVDRSDLLSMLLQARDEEGDGSGMNDLQARDEALTILLAGHETMATSLTWTWYLLS
                     QYPEAEAKLHAELDTVLAGKLPSINDVAHLRYTRMVFAEALRLYPPVWLMGRQALNDY
                     KIDSYVIPVNSLVFISQYVTHHDPRYYPDPFRFDPERWTPEAEDALPRFAYFPFSGGP
                     RQCIGEQFAWTEGVLLIATLAQKWQMRLVPGHPVEPHPLITLRPGHGMRMKLERRKLV
                     PVNQEVRAPLELTNL"
     gene            complement(3033..4265)
                     /gene="fabF"
                     /locus_tag="VI935_03255"
     CDS             complement(3033..4265)
                     /gene="fabF"
                     /locus_tag="VI935_03255"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HLE24649.1"
                     /translation="MERRVVITGIGLITSLGSGTQKTWKSICCGRSGIRRIQSFDPSN
                     LKTQFAGELTDFNPLSFMDAKDIRRTDPFIQYAIAATRLALEDAGLEITEELSSSVGA
                     IIGSGIGGVDSFRKNFLEMYERGPNRVSPFFITSIIPNMASGYVSMIFNAKGPNSCPV
                     TACAAGAHAVGDGMRIIEREEADVMIVGGAEAAIQPIGIAGFNAMRALSTRNDEPERA
                     SRPFDRDRDGFVMGEGSGILILEELEFAKRRGARIYAEIIGYGMSGDAFHITSPSLKG
                     PVSAMKQALKGAAISLEEVDYINAHGTSTPQNDPNETRAIKEVFGELAYKIPVSSTKS
                     MTGHLLGAAGGVEAAFTALSIYEGIIPPTINLDNPDPECDLDYVPYTSRDAGVRVALS
                     NSFGFGGTNVTLVFKSFD"
     gene            4842..6026
                     /locus_tag="VI935_03260"
     CDS             4842..6026
                     /locus_tag="VI935_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLE24650.1"
                     /translation="MDTRLAKLFPLTIPISYIIHMVSIARRNLFHDKTRLIITIVGVI
                     FAVILMTVQIGVYIGFVSSASQLIDNSPADVWITSKNTVNFDSAKPFSENKINKVREH
                     NDVLWVEKMAQTWGLLKLRSGATESVQIVGFNPESGIGGPWKMKEGNIKEVKVGNSII
                     LDESSLKKLNVKLGDKLEIFNTQTEVVGISQGVRSFTTYPIAFTAYKTAKELSRIIRE
                     GQTTFILVKLKPGVDAERFAEKLRERMSGVDIYTKGEFSNKTRLYWSLQTGIGIGVGI
                     TVFLGFIVGTIIVGQTIYSSTMEHIREFGTLKAIGATNMDIYKIIFEQALINACIGYA
                     LGFILTLITKNLYDRFRVDLILTPELIILMFFITVSMCLLSSFISIQKVAKVDPVIVF
                     KA"
     gene            6151..6840
                     /locus_tag="VI935_03265"
     CDS             6151..6840
                     /locus_tag="VI935_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLE24651.1"
                     /translation="MMKQIEVADIQKIYEEGSVRVHAVKGVTLSVDAGELVLLMGPSG
                     SGKTTLLSMMGCILKPTKGSLRIMGEEVINWNENSLPLTRRRHIGFIFQHFNLLSALT
                     ALENVEVSLNLKGIKGKEGNRIARDLLEQVGLGDRCDFLPMDLSGGEKQRVSIARALA
                     GKPSIILADEPTGNLDSQTGRKVIELIKQLAVKESKSIVIVTHDSRITDLADRIYHIE
                     DGILTNGYRSL"
     gene            6898..7965
                     /locus_tag="VI935_03270"
     CDS             6898..7965
                     /locus_tag="VI935_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLE24652.1"
                     /translation="MSKKKIGIYITALIVLVLTLAGLASRNKSTNNEVKIKKGNIDLI
                     VAAEGKVEGIEDVDLAPKTLGRIKEIYVKEGDYVKDGDLIAVLENDELKAQVEQAKAN
                     VLRAEAELRESKQNWNRSTALFDKGIVSSSQLDTAKMKYDVTHSQLKKAEADLKYANA
                     LLENTYIRAPFSGKIIKRFLDPGETITLEKLVPIVNIADVNKIMVRAEINDTDIGKVK
                     IGQKAIVTTDAYTGEEFNGKVVEISPAVGKKAILSDDPAQMVDTKVLEVKIELDSGQK
                     LNLGLNVDVIISANNKEDVLILPLKALIHRDGMPYVKVKRNGKYEEKMITTGLTDEEN
                     IEVSDGLEEGDTVLLQNHSGF"
     gene            8167..9135
                     /gene="corA"
                     /locus_tag="VI935_03275"
     CDS             8167..9135
                     /gene="corA"
                     /locus_tag="VI935_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HLE24653.1"
                     /translation="MPLRYIIWDETGKIVKEGDNRDAVATSLPHENLRLWLDIESHDK
                     EEIEWLGTIFNFHYLALEDCINLNQRSKLEEYDGYMFSVLHLSSLDSDGRIETQELHI
                     FLGSQFIVSIHNKPMEIVDSCLARCKGEPHTLEKGCDFIFYMLSDTLVDRYFPLLDKI
                     EDELDDLEDKVISNPTREFLTKIFLLKQDLIALRKIVGPLREVFSLIYRRDYGLIKEK
                     TFLYIRDVHDHLVRIHDIIDTYRDMLGNMLDVYLSTVSNNLNQVMKRLTLIATIFMPL
                     GFVVGFFGMNFEKMPFDSNILLFLALIICLILPIGMIFWFYKHKWF"
BASE COUNT         2841 a         1952 c         2064 g         2632 t
ORIGIN      
        1 atacgaccaa ctaatgaaga tgggagattt gatgtatatt tttgtagcca aaaaattggt
       61 gaaattaatt tagcttgcta tcatgaaaaa ctgtaaccca tgtctccgaa cacctgttac
      121 ctatgtcccc ggtctataca agggggatac agggggattt ctagaactta actttttgct
      181 tcatctcaaa tcctaattgc ttcacatact catttccaaa tcccctctaa ctctccttta
      241 gaaaaatgga gaattagaat ccccatttgt aaagaggaga atccttaaag gagatggatg
      301 aagatcttcc tctggctagg aatgtgaatc tgtaggaatt gggccaataa cctgccagcc
      361 tttccaatcc attgcactgc aacattgttt catacgttcg cggcagacgc ccgtaatggc
      421 aggaaggctt tcctcgggga tgtaactaag ctttgattcc ggcatagtta acggcatacc
      481 gagttcttcc agctttgcaa gtatggcctc gctttcatca ggcagtccta ggaattcgaa
      541 aaggtgacgg agggaggtat ggggttccag aatgagggcc gagtagtcca ggaaacatcg
      601 cgtgttagaa gaacccaatt tgcccaaatc ccttaacgcc tgctcaatcc attgaagatg
      661 atccatagcc atggcgagtg ggtctggtga ggtaggttta agattcaccg ttcggtttgc
      721 ttgctccata aaatagccca tcccggattg ctcgaagtgc tggtttcttc cgactagacg
      781 gtgatagaga agcgaagggg actgtgccat ttcttgccag accttgatga acttcagctt
      841 tgaattgacg caggcgactg ggtctcgata caaataaatg aatcgcgcgt ctggaaagag
      901 ggaaagaacc tccggtatct gaattgtaag gtaggggacc ttccacagaa aacgcgtctt
      961 gccttccaaa tataatagct tccgcatgta gttcctaatg tcttccggag tcgttgggtt
     1021 ataaagcact tgcttcttat caaaatagcg ggagattaga ccctgcgcct cctgcggggt
     1081 atcaaagccg acaataatgc gatctatcct tcggtaggcg cggctacgaa taaggggacg
     1141 caaatattgt ttcgctagaa tgggaatcaa gtcaaacata tcggaatagg ttcttggcgt
     1201 cgcaacagtt gagtgcaggc ttagcatctg ctggaggatc gtagttcctg aacggtgggg
     1261 accgatgatg aaaatcggtt tcctgagctc aatatcctcc cacttcttcc tgtaaaaccg
     1321 gtcttccacc ttagacaagg acacctttaa tttagctaca aaggagttaa agaaatgttc
     1381 attttgaaaa taaccatatg caagatacca gaacaggcgg tacttccatg tggggttttc
     1441 cgacctcaca ttcgtatatc ctaatgatgt atttgtagac attaacctat atgtcaactc
     1501 atttttaaag attagtaagc tcaagaggag cacggacctc ttggttgacc ggtaccaatt
     1561 ttcgccgttc cagtttcatc cgcatgccgt gtccgggacg caatgtgatt agtgggtgag
     1621 gctcaaccgg atggccaggg acaagccgca tctgccactt ctgtgctagg gtggcaatta
     1681 acagaactcc ctccgtccac gcaaactgtt cgccaatgca ctgcctggga ccaccgctaa
     1741 aggggaaata ggcaaacctg ggtagcgcat cctctgcctc aggggtccac cgctcggggt
     1801 caaagcggaa gggatctgga taataccgcg ggtcgtggtg cgtaacatat tgacttatga
     1861 agacaaggga attcacaggg atgacatagg agtcaatctt gtaatcgtta agggcctgac
     1921 gccccatcag ccacactggt ggatatagtc gcaatgcctc cgcaaagacc atccgggtgt
     1981 atcgcaagtg cgccacatca ttaatcgagg gaagcttacc cgccagcact gtgtcgagtt
     2041 cggcatggag ttttgcctct gcctcagggt actgtgaaag cagataccag gtccaagtca
     2101 gtgaggtggc catcgtttcg tgtcctgcta aaaggattgt aagtgcctca tcacgcgcct
     2161 gtaaatcatt cattccacta ccatcaccct cctcatcccg ggcttgcagc agcatcgaga
     2221 gaaggtcact acggtctaca ccactcgccc ggcgctcatt gatcatccta tagatagtat
     2281 catccaggcg atcctttgct ttccggaagc ggcgattgct gggaagtggg aacctatcca
     2341 gcaattcaga aaatggaacc aagagcatcg gagccctttc cataattatg gtccatgcct
     2401 cgccgatctc ctttgcctct gattcgatgt cggcgtcgaa aagcgccttc ccaactattg
     2461 ccagagtcag gcgcatcatc tcctgtgcta tgtccagtgt tgcaccatcc tgccagcgct
     2521 ctcgtatcct cgctccgtat tccatcatca cagctccata cgctgcgata cgctgccgat
     2581 gaaaggctgg ctgaatgagt cgtcgctggc gacggtgata atggtgatcg ctggtagtaa
     2641 ttaacccctc gccccctaaa aactttaacc tttgtaagat ccgaccctta gcaaaattct
     2701 gataattatt caccaacacg tcctggatgt aatcaggatg attgattaag aaagcattta
     2761 agggtccgat cctaaaatgg gcgatatcgc catattcacg ggcaagatta agcagaaagc
     2821 tgtgtggttc gcggtagaat gcaagcatat cgccaatgag cgggatgccc ttagaaccgg
     2881 gtggaaagct ggcggttgtc atgtaaaact ccttaataaa atgatgcatg atacattatg
     2941 actcttgtat cctttatcct gcatcctgca tcctgcttat tttcattcgt gtctatttgt
     3001 gtcaattcgt ggctggataa cattgtggca ggctaatcga aagacttaaa aacaagggtc
     3061 acatttgtcc cgccaaatcc aaacgagttg ctaagggcta cccttacccc ggcatccctg
     3121 gaggtgtagg gaacataatc caggtcacat tcggggtcgg gattatctag attaatagta
     3181 gggggtatta tgccctcgta tatagacagg gctgtaaatg ccgcctcaac accgcctgcc
     3241 gctcccagta gatgccccgt catagacttt gtggagctta caggaatctt atatgccaac
     3301 tcaccgaata cctcctttat agccctagtc tcgtttgggt cgttttgagg ggttgaggtt
     3361 ccgtgtgcat ttatgtaatc aacctcctca agactaatgg cagcaccctt tagtgcctgc
     3421 ttcatagcac ttacgggacc ctttagagat ggggacgtta tgtgaaaggc gtctccactc
     3481 ataccgtagc caataatttc agcatatatt ctagccccac gcctttttgc aaactcaagc
     3541 tcttcaagaa ttaaaatccc ggagccctcc cccataacaa atccatctct atctctatca
     3601 aagggtcttg aggcgcgctc cggctcatcg tttctcgttg atagtgctcg cattgcgtta
     3661 aaccctgcaa taccaatagg ttggatggct gcctcagccc ctcctactat catcacatct
     3721 gcctcttctc tttcaattat tctcatcccg tcccctacag catgggcacc agcggcgcag
     3781 gctgtaactg gacagctatt gggccccttc gcattaaata tcatagagac gtagcccgat
     3841 gccatattgg gaataataga tgtgataaag aatggggaga ccctgtttgg acccctctca
     3901 tacatctcta aaaaattttt tcgaaatgaa tcaacccctc ctatccccga gcctatgatt
     3961 gcacctacac ttgaggaaag ctcctctgtg atctcaagcc ctgcatcttc aagtgcaagc
     4021 cttgttgcag ctattgcgta ttgtatgaat gggtcggtcc tccttatatc ctttgcatcc
     4081 ataaaggaaa ggggattgaa atccgtgagt tctccggcaa attgggtctt taggtttgag
     4141 ggatcgaaac tctgaatacg tcttatcccg gaacgcccac aacatatgct tttccaggtc
     4201 ttctgggtac ctgaacccag cgaggttata agccctatcc ctgttataac tactcttctc
     4261 tccatcccta cctctctaaa agtcaagtct atgacattct ggaatataat gtatactgca
     4321 tactcaactt aaattacggg ttacacctag catatctgaa tataaacacc tttgatttat
     4381 caactaagga agtaaaatat atttcttaag ttgatattat taacataaga acctaactta
     4441 taacatataa acaatttaat agcaacaatt tctataaaaa ttccccccca ataagttcgt
     4501 tttcaggcac gcatgcggat agcgaccatt gcaatgcctg ttgttgtgtt atttctgtca
     4561 catcgcgtta tatcaattcc ggctgaatgc ttgtcttata accctataac ttgtgacgct
     4621 tcagattttc agtggatatg tatccgttca ttctgaactt gtcgaagaat gcccattttg
     4681 tcctagacga gcaccgttca tccttcgaca ttggataagc tcatggatca ggacgaacgg
     4741 tctgttttga ccatataaat tgcaaacact agttaaccgg aatcggtatt aaacctaagc
     4801 ctctctttac accttgtacc aaatgtggca tatggatagc tatggatacc cgtttagcga
     4861 aactatttcc acttaccatc ccgatcagtt atattattca tatggtatcc attgcgaggc
     4921 gaaatctctt tcatgataaa actagactaa tcattaccat cgtgggtgta atctttgcag
     4981 tgattcttat gactgtccag ataggagtat atattgggtt tgtaagcagc gcttcacaac
     5041 tcattgacaa ttctcctgca gacgtctgga taacgtctaa gaacacggta aattttgact
     5101 cagctaagcc cttttcagaa aataagataa ataaggtaag agaacacaat gatgtactct
     5161 gggttgaaaa gatggcccag acctgggggc ttttaaaact caggagcggt gctaccgaat
     5221 cagttcagat agtcggcttc aatcctgaaa gcgggattgg tggcccctgg aagatgaaag
     5281 aaggtaatat caaagaagta aaagttggaa attcaattat tctagacgag tcttctttaa
     5341 aaaaattaaa cgtcaagctt ggggataagc tggaaatatt caatacgcag acagaggttg
     5401 tgggaataag tcagggggtt agatcattta caacatatcc tatagcgttt accgcataca
     5461 agaccgcaaa agaactttca cgaataataa gagagggtca aacgactttt attcttgtaa
     5521 agcttaaacc aggagtagac gcggaacggt ttgcagaaaa actcagagaa agaatgagtg
     5581 gtgttgatat ctatactaaa ggggaattta gtaataaaac aagattgtac tggagtcttc
     5641 aaactgggat cggaatagga gtcgggatta cggtatttct cggattcatt gtaggtacaa
     5701 ttattgtagg acagacgata tattcatcca ctatggagca cattcgcgaa tttgggacac
     5761 tcaaagcaat cggggctaca aatatggata tttataaaat tatttttgag caggccctaa
     5821 ttaatgcttg tattggatac gcacttgggt tcatactcac gcttataaca aaaaatctat
     5881 atgaccgctt cagggtagat ttgattctga cccctgagct tattatctta atgtttttta
     5941 taactgtatc tatgtgtctt ctttcttcat ttatttcgat tcaaaaagta gcaaaggttg
     6001 atccggttat tgtgtttaaa gcttaatgta gtatttgatg cgcagtgtgc atcaaatgga
     6061 aataagaaag gtagtatttg atgctgtact attcggtgcg cagtttgcac tgaatggaaa
     6121 taaacagtgt gcataaaatg gaattaagag atgatgaaac agatagaagt cgctgatata
     6181 caaaagatat atgaagaggg ttccgtaagg gttcatgctg taaagggggt aaccctatcc
     6241 gtagatgcgg gtgagttggt tcttcttatg gggccttcag gaagtggaaa gactacactt
     6301 ttatcaatga tgggatgtat cctaaaacca actaaaggga gtttgcgcat tatgggagaa
     6361 gaggtaataa attggaatga aaactctctc cccttgacga gaagaaggca catcggcttt
     6421 atctttcaac actttaatct tctaagcgct cttaccgccc ttgagaatgt agaggtttct
     6481 ctaaacctaa aaggtataaa gggaaaagaa ggaaatagga ttgcaaggga tttgcttgag
     6541 caggtagggc taggagatag gtgtgatttt ctgccgatgg atttaagcgg cggagaaaaa
     6601 cagagggttt caattgccag ggcgctggct ggcaagcctt caattattct agcagacgag
     6661 cctactggta atcttgattc tcagactgga cgaaaggtaa tcgagctaat caaacaactc
     6721 gctgtaaagg aaagcaaatc aatagtaatt gttacacatg atagtagaat cactgatctt
     6781 gctgatagga tttatcacat cgaagacggc attttaacaa atggatatcg tagcctataa
     6841 aagattatat tttaaaatgt attgttgtta aaaaatctcg acaataggtt aactactatg
     6901 agtaaaaaga aaataggaat atatataact gcgctaatcg tacttgttct aacactggct
     6961 ggattagcaa gccgaaacaa atctaccaat aatgaggtta agatcaaaaa gggaaacata
     7021 gatttaatcg ttgctgcaga gggaaaggtt gaaggaattg aggatgtaga tctcgctccc
     7081 aagactctcg gaagaataaa ggaaatatac gttaaagaag gagattacgt taaggatggc
     7141 gatctgatag cagtacttga aaatgatgag ttaaaagccc aggttgagca agcaaaagct
     7201 aatgtcctgc gtgctgaagc tgagctaagg gaatccaaac aaaattggaa tcgatctaca
     7261 gcgctattcg ataagggaat tgtctcaagt tcacagctcg acactgcaaa gatgaaatat
     7321 gatgtaacac attcacaact caaaaaggcc gaagctgatc ttaagtatgc caatgctctt
     7381 cttgaaaaca cctatatcag agccccattt tctggaaaaa ttataaaaag atttctcgac
     7441 ccaggagaaa ctattacact tgagaaatta gttccaatag tgaacatagc agatgtaaat
     7501 aaaatcatgg taagagccga gataaatgat acagatatcg gcaaggtaaa aataggacaa
     7561 aaagcaatag taactacaga cgcatacact ggagaagaat tcaacggcaa agttgtagag
     7621 ataagtcccg ccgtgggtaa gaaggcaata ttatctgatg accctgctca gatggtagat
     7681 acaaaggttt tagaggtcaa aattgagctt gattcaggtc aaaagcttaa tcttgggctt
     7741 aatgtagatg taattatctc agcaaataat aaagaagatg tccttatcct tcctttaaaa
     7801 gccttaatac atcgagatgg aatgccctat gtaaaagtaa agagaaacgg caagtatgag
     7861 gaaaaaatga taactactgg cctaactgat gaggaaaata tcgaggtaag tgatgggctt
     7921 gaagaagggg atactgttct tcttcaaaac catagtggat tctgaaatta atagccctgc
     7981 agaaggctgc agggtatggt cttaaataat accgtcacaa atgtcattgc gaggagccct
     8041 tcgtctagct caggataaac aacgcgacga agcaatctaa aaacgagatt cctcgcttca
     8101 ctcggaatga cagcatagtg tgaaccctgt agcaaggact ctgatattta aaaaaacaaa
     8161 attctgatgc cccttaggta cataatctgg gatgaaacag gaaaaatagt taaagaagga
     8221 gataaccgtg atgcagttgc tacttccctt ccacacgaga accttcgcct ttggctagat
     8281 atcgaaagcc atgacaaaga agaaattgag tggctaggta caatttttaa ttttcactac
     8341 ctcgcgcttg aggactgcat caacttaaat cagcgttcta agcttgaaga atacgatggt
     8401 tatatgttct ctgtacttca cctttcatct ttagatagcg atggaaggat agaaacccag
     8461 gaactacata ttttcttggg ttctcaattt atagtttcga tccacaataa acctatggaa
     8521 atcgttgatt cctgtcttgc acgatgcaaa ggagaacctc acacgcttga aaagggttgt
     8581 gattttatct tttatatgtt gagtgacacg ctagtagaca ggtattttcc gcttcttgat
     8641 aaaatcgagg atgaacttga cgacctagaa gataaagtaa tttccaatcc aacccgcgag
     8701 tttctaacaa agatatttct acttaaacag gatttaatag cattacggaa gatcgtaggt
     8761 cctcttaggg aggtctttag tttgatttac agacgtgatt acgggcttat taaagagaaa
     8821 acctttctct acattcgtga tgtccatgac catctagtta gaattcatga cataatagac
     8881 acctatcgcg atatgcttgg gaacatgctt gacgtttacc tctcaacggt atcgaataat
     8941 ttaaatcaag tgatgaagcg gcttactcta attgcgacaa tctttatgcc tcttggattt
     9001 gttgttgggt tttttgggat gaattttgaa aagatgccat ttgatagcaa tattctactc
     9061 tttctagctt taattatttg cctaatactt ccaattggaa tgatattttg gttttataag
     9121 cacaagtggt tttaaaaaaa tttctgattg aaatgagcca aatcccggca atttcaactt
     9181 gaaaaataat atctgcgctt gtttgatttg taatcagagt tgaatgaaaa atgccgaact
     9241 tgtgaataaa gtaaatacca tttagatacg aaaaaattaa cccctgggct agatgaacaa
     9301 aggacatatg catctcacta tataaattcc ataatatgga aattattaac gactttttgt
     9361 tacaccacaa tttgattgaa ttgtataata tataaatagt aaggataatt agaagatgtg
     9421 cgatttagtt tttgataaag aagatattaa gcaaattgag gctagaggac ttacgctcca
     9481 aaaggtcat
//
LOCUS       DATDRL010000097        11370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1195216, whole genome shotgun sequence.
ACCESSION   DATDRL010000097 DATDRL010000000
VERSION     DATDRL010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11370
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195216"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1791
                     /locus_tag="VI935_03280"
     CDS             <1..1791
                     /locus_tag="VI935_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HLE24654.1"
                     /translation="TTVICTDKTGTLTTNQISVVRLWFNNSLFEIEGSGYDPSGQILY
                     NGSTLSQDEIKKSGMEALIKIGALCNNAEVLAPSDEKPFWNIGGDPTEGALLVFAKKA
                     GFDYKDIKDKNPRIFQISFDSVRKRMSTINDLEGENIWANVKGGPREVLSLCSSVYLD
                     DEKQPLTDEVRQRILEHLDNFAKEGLRVLALAYKKVEKKDTYSVSEVENELTFVGLTG
                     MMDPPRPEVPMAVGLCQSAGIKVIMITGDYGLTAQSIARKAGLVSDDNTRIVTGTELT
                     EMNDGELKEALQKKEVIFARVSPDHKMRIVDALKDIGEIVAVTGDGVNDAPALKKAHI
                     GIAMGLRGTDVAKEAATMILTDDNFASIVAGIEEGRAIYSNIKKFVTYIFASNIPEII
                     PFILFVVFKIPLPLTIMQILAVDLGTDLLPALGLGAENPEPGVMNQPPRSLKKRLLDS
                     PLLLRAYGFLGIIESLACMAGYFFIYYSSGWRPGLELISSGPIYLKATTMCLAGIIAT
                     QIGNVFACRTERESVFKIGFLNNRSVLLGIAAEVVIINIIVYLPPFQKIFNTFSLDPV
                     DWAFLLAFPPIIFLAEEGRKYIIRLNQKKK"
     gene            1914..2663
                     /locus_tag="VI935_03285"
     CDS             1914..2663
                     /locus_tag="VI935_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HLE24655.1"
                     /translation="MLDNIVIQVFVVALLTDLATGLGALPFAFFRHPSVRWQGIASAL
                     AGGMMISASVFSLADQALRRGSVWEVVLGMLAGAAFFAWIASRVEDNEWRIANLNTVD
                     SRQAVLIVVTMFIHSIPEGVAIGVGYATGELRFGFLLALAIAVHNIPEGMAVTLPLRA
                     KGVSLWRCAWYAILTSVPQPLLAVPSFLLVSVFKPLLPSGLGFAGGAMIYLVAAELIP
                     DSLRGCSRQETAWGVITGLVLMLLITSGLGL"
     gene            complement(2774..3193)
                     /locus_tag="VI935_03290"
     CDS             complement(2774..3193)
                     /locus_tag="VI935_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLE24656.1"
                     /translation="MLVKNWMRRNPIIVEPETGVKSAFLLTKKHRIRQLPVVRDGVLI
                     GIVTDRDFRKPELPHRFYTADEVYRLEDKFTVGDIMTMEVITVTENTSIEEAARLLLE
                     HRINGLPVISENGRLIGIITTSDILGAFLRLYKFGNW"
     gene            complement(3204..3626)
                     /locus_tag="VI935_03295"
     CDS             complement(3204..3626)
                     /locus_tag="VI935_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLE24657.1"
                     /translation="MLAKDVMKTEVVTVLLSAPITEAALSMREEDIGALIVVDDEGRP
                     VGIITDRDIVVSVIANSKNPLEIIVEEVMTKKLIVVQEDASIFEILKILAKNNIRRVP
                     VMRRGKLVGIVSVDDLIVVITTELSNLALALSGTSKVL"
     gene            complement(4009..4803)
                     /locus_tag="VI935_03300"
     CDS             complement(4009..4803)
                     /locus_tag="VI935_03300"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HLE24658.1"
                     /translation="MYPLLRLFGNPSEGYLITIHTYKVMFILGILIGLSYAVYMIRRL
                     GESEERMIYMSLLGVFFGILGSKVLYIIYKFEHFLEHPHSLFEKGGGGAVFYGGFAAG
                     LLAALYYMKRTKLNMKRYLDVIAPASILGQGIGRLGCFSAGCCYGKPTSLPWGIIYPE
                     ELRGVTLIPERYLGVLSLHPTQLYSALGNIIIVLVLTYILLKRKRFDGEVFAYGLILH
                     SGFYFSMGFLRDAGSQAPMFGTLAVTQIVYILALVFGVYLIYKFKA"
     gene            5506..6165
                     /locus_tag="VI935_03305"
     CDS             5506..6165
                     /locus_tag="VI935_03305"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HLE24659.1"
                     /translation="MPKSQVLLFAVFLFLGKPASEAASLEPVLPQDPESRPQLKPAKE
                     ETPEWMRPWLQEIGAMIGYGLAHEIFGTRDGRRQYLILNPRWGMFLTGVIGKVPYKGA
                     LEFLVEPIAMFQFEPNNRYALGLSALLRYNFWTPSRRVTPFFDAGGGVMWTNLGLPDL
                     GSNFNFQLQFGPGLHVHFNRGTALTFQYRFHHISNAETAKPNFSINSSLLLIGVTFFL
                     L"
     gene            6348..6476
                     /locus_tag="VI935_03310"
     CDS             6348..6476
                     /locus_tag="VI935_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24660.1"
                     /translation="MGGQYGILKESATSITGLENPLYIVYSSNVLSANRVVCNLDE"
     gene            complement(6832..8181)
                     /locus_tag="VI935_03315"
     CDS             complement(6832..8181)
                     /locus_tag="VI935_03315"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLE24661.1"
                     /translation="MHKCNVLVVDDDEELVNAVKELLERKGYEIITAFSGKEAIDKMS
                     SFPQICAALLDLVMPMMDGFTLLDEVKENHPETNAIIITGHGTVPNAVEAIKRGAMDF
                     ITKPFDKDLLLKKLEVIKKTHELENRITELKGLLSEKYGFEQIISSSRLMGGVFERAT
                     AAARTDASVFIVGETGTGKELLAKAIHLKSERSNQPFVAVNCAAIPKELMESELFGYK
                     KGSFTGAMKDHDGLFMAANRGTIFLDEIGEMPRDLQVKLLRVLEEHKIRPIGYTSEIQ
                     VDIRIIAASNRSIEELKNTYLREDLFFRLAVVVIELPPLRERREDIPLLTEYFIRKFN
                     DRYSRSIKGVSEGVFTSLYRYHFPGNIRELENLIEGIIAISPPEKETITEKDLKAHLV
                     WQETKPLEHTLLSLEKLERFALEQALRESQGNKSKASEILGISRDTLYRKLKQFGIE"
     gene            complement(8174..9712)
                     /locus_tag="VI935_03320"
     CDS             complement(8174..9712)
                     /locus_tag="VI935_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLE24662.1"
                     /translation="MRDKDKISKQVVEVVEVRQGVAESEMEGGLEQEKFRLIVELAPN
                     AILMVNQKGEIVLVNSQTEKLFGYNREELIGRTVEILVPQRFQTKHSEYRKSFFVNPQ
                     ARLIGTGRDLFAMRKDGGEFPVEIGLNPVKTEGGVLVLATIVDITERKRAEQLLRESE
                     ERLQAIMDNTTDAIMVYDEDGMVIAINREAERLFCSNNKTGLKNIWDVIPLENRADFS
                     NILKSVKEGSRLLDYEIEKIIDNGERIPVSIGLVYMTDGSGMFIETIRDIRERIKLRN
                     KIVELEKAQVIGKLAEGIAHHMGTPLASMLLRVQMLKEDIDEIPESKKFMERLDSIEK
                     QIFYAQRVMQRLLKFASKPENVKRPENVSYLIEEAVDIIKPLLRKEGVELELFLDEDV
                     KVWADSNLLLLVFSDIMMNAVDAMHSGGRLSITISNVNPDGQIQIKMSDTGVGISRSV
                     LPLIFDPFFTTKPAGKGTGLGLSVAKRIIQDHNGEISIESTEGEGTSVLIRLPIYSEE
                     RTVA"
     gene            complement(9898..10512)
                     /locus_tag="VI935_03325"
     CDS             complement(9898..10512)
                     /locus_tag="VI935_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLE24663.1"
                     /translation="MDFANFKHNEMIIYPREIILKDKTTIILRPLEEDDKEGLLDFFS
                     KIPRSDLIIFKDDVGKLETVESWFTSSKYDKVFQLIALRGKKIIGKGTLHKEGLYWRF
                     STEIKLIVEPEHRGRGLGLQMFKMLLAEGLNQNFEKVVVRFTNDNKSFIRILDHFGFK
                     PEAVLTCYIKDEQTEIKKDLLIASYNLKDWARRFEFYNIIYGGK"
     gene            10747..>11370
                     /locus_tag="VI935_03330"
     CDS             10747..>11370
                     /locus_tag="VI935_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLE24664.1"
                     /translation="MEISKILWASDGSMESDMALQYVELLASKYGSEIVGLSVVQPVD
                     IVRLKVPVDVKKELFSIQALLEKKEYNRINKALKQLELKDTKLDICIETGIPHKEILR
                     VAREEKVDLIAMGKRGLGLKDRILLGSNTNKVLRMANAPVLAVRHKKGDKIDIKKILV
                     PTDLSELTSISLDFAIDIANRFNSTIYLLHTLELHHSYEISTSKIIEE"
BASE COUNT         3225 a         2454 c         2207 g         3484 t
ORIGIN      
        1 acaactgtta tctgtactga caagactggc actcttacta caaaccagat aagcgtagta
       61 aggctttggt ttaacaatag tctttttgaa atcgaaggat caggatatga tccaagtggt
      121 caaatacttt ataatggctc tacgctgtct caggacgaaa taaaaaagtc agggatggaa
      181 gcgctaatta agataggcgc cctttgcaat aatgctgagg ttctagctcc gtcagatgaa
      241 aagccattct ggaatatcgg cggcgaccca actgagggag ctctactagt atttgccaag
      301 aaggcgggct ttgactataa agacataaag gataaaaacc caagaatctt tcaaatctcc
      361 ttcgattctg taagaaagcg tatgtctact ataaatgatt tggagggaga aaatatctgg
      421 gctaatgtaa agggtggacc tcgtgaggtt ctttcgctct gtagctcagt ttatctggat
      481 gatgaaaagc aacccttaac agacgaggtt agacaaagaa ttttagaaca ccttgataat
      541 tttgctaagg agggtctaag ggttttggcc ttagcatata aaaaggtgga gaaaaaggat
      601 acctactctg taagtgaagt ggagaatgaa cttacctttg tagggcttac aggaatgatg
      661 gatcctcctc gtcccgaagt tcctatggcc gttggtttat gtcagagcgc aggcataaaa
      721 gtcattatga tcacgggtga ctatggtctt actgcgcaat ctatcgcaag aaaagccggt
      781 cttgtaagtg acgataatac caggatcgtt acaggtactg aacttacgga aatgaacgat
      841 ggtgaactta aggaagcctt acaaaaaaag gaggttatat ttgcaagggt ttctcccgat
      901 cataagatga ggatagtaga cgctcttaag gatataggag aaatcgtggc tgttacaggg
      961 gatggagtta acgatgctcc tgctcttaaa aaggcgcata taggcattgc aatgggtctc
     1021 cgtgggacag acgtagcaaa agaggccgcc acaatgattc tcaccgatga taattttgca
     1081 agtattgtag caggaattga ggaaggaagg gcaatatact caaatataaa gaagtttgta
     1141 acatatattt ttgcctctaa cattcctgaa ataattccgt ttatcctttt tgtcgtattt
     1201 aagattcctc ttcctctaac tataatgcaa atattagcag ttgatcttgg tactgactta
     1261 cttcctgccc taggtctcgg agctgagaat ccggaaccag gggttatgaa ccagccacct
     1321 agatcattaa agaaaaggct attagactca cccttactac ttcgggctta tggttttctg
     1381 gggataattg agtctctagc ctgtatggcg ggatacttct ttatttacta ttcttccggg
     1441 tggcgtccgg gtttggaatt aatttcatca ggcccgatct acctaaaagc cacgactatg
     1501 tgtctagccg gtatcatcgc aactcaaata ggaaatgtct ttgcctgccg cactgaaagg
     1561 gagtcggtat tcaaaatagg ttttttgaat aaccgatcgg ttttgcttgg tattgcggct
     1621 gaggtcgtta taatcaatat aatagtctat ttacctccct ttcaaaaaat atttaataca
     1681 ttctctcttg atccagtgga ttgggctttt cttcttgcat ttccgccaat tatctttctt
     1741 gcggaagagg gaagaaaata cattattaga cttaatcaga agaaaaaatg aacatctttg
     1801 ttgtgggttt agttaatagt tttaggtctt tttgcataca taaagaggag gttgcaagct
     1861 tttcttctta gttttgtccc tatgaggttt gttataataa ttttctcgtc gtcatgctgg
     1921 acaatatagt tattcaagta ttcgtagtag cacttctaac cgaccttgca actggtctag
     1981 gcgcgttgcc gtttgccttt ttccgacacc cgtcagtaag gtggcaaggt atagcgtcgg
     2041 cacttgcggg cgggatgatg atttcagcgt cagtatttag cctagctgac caggctctcc
     2101 gtcgaggtag cgtgtgggaa gttgtattgg gaatgctagc tggcgctgct ttttttgcct
     2161 ggattgcaag ccgagtggag gataacgagt ggcggattgc caacttaaac acagtagact
     2221 cgcgtcaagc tgtattgatc gtagtgacta tgtttataca tagcatcccc gaaggggtgg
     2281 cgattggtgt aggttatgcg acaggcgagc taagatttgg ttttcttctt gcacttgcaa
     2341 ttgctgttca taacattcct gaggggatgg ctgtcacgct tccacttcgc gctaagggcg
     2401 tatcgctatg gaggtgtgcg tggtatgcaa ttttgacaag cgtgccacaa cccctccttg
     2461 ctgtaccatc atttcttcta gtatctgtat tcaagcccct gttaccatcc ggattagggt
     2521 ttgctggcgg cgctatgatt tatctagtcg ctgccgaact gataccagat agtctccgcg
     2581 gttgctctag acaagaaact gcatggggtg taataacagg tttggtcttg atgcttttaa
     2641 tcacgtcagg tttgggattg tagcgaactt ttataaggaa taaaatcctc ttctgatatg
     2701 atttcaaaat agtgattttt agacgcaaat tttctcacct gttttttatc taagagaatt
     2761 acgaatgtct tattcaccag ttcccaaact tataaagcct caaaaaagcc cctaagatat
     2821 cagaggtggt tattattcca ataagccttc cgttctcaga aattacagga agaccattta
     2881 ttctatgctc gagaagtaat ctcgctgcct cttcaatact cgtattttcc gtgactgtta
     2941 taacttccat cgtcatgatg tctcctaccg taaacttgtc ttctaaccta tatacctcat
     3001 ccgctgtgta gaatctatga gggagctcag gttttctgaa gtctctatct gttacaatgc
     3061 cgatgagtac accatctctt acaaccggga gctgccttat cctatgtttc ttcgtaagta
     3121 aaaaagcaga tttgactccc gtctcgggtt ctacaattat tggatttctc ctcatccaat
     3181 tttttacaag catttcctct tgcttaaaga acctttgatg tgccacttaa agccaaagct
     3241 aggttagaaa gctctgttgt gataacaaca attagatcgt ccacagatac tattccaaca
     3301 agctttcccc ttctcataac ggggaccctt cttatattat tctttgcgag tattttgagt
     3361 atctcaaata tactcgcatc ttcctggact acaattagtt ttttggtcat aacctcttca
     3421 actataatct caagggggtt tttactattg gctatgactg aaaccacgat atccctatca
     3481 gttattatcc ctacaggcct accttcgtca tccactacga taagggcacc tatgtcctct
     3541 tccctcattg atagagcggc ctctgttatc ggagctgata gcaacactgt aacaacctca
     3601 gttttcataa catcctttgc aagcatattt aatcctccgt taatttattt aatccgttaa
     3661 gataataggc aatttgtatg ccagctaatc tcaaccatta tataattata ctattttact
     3721 ttggatactc tattctatat aaaattttgg ttatagttgc tgttagatgt cagaaaatct
     3781 tgcagaaatg tattactgat aggttcttat cacattttga gagttaatgc tgtaagtccg
     3841 tctaaccact taattattct tttcaatcac tataaggctg gacattccta tcccgcttcc
     3901 atagatgcag tccttgggtg taaaggttct aatgatacag attgttagag cgatgaagaa
     3961 aacggtggaa atgtagagta gttataggca aagataaagc tataccaatc aagctttaaa
     4021 tttatatatt agataaacac caaacacaag ggccagaatg taaacaattt gtgttaccgc
     4081 tagtgttcca aacattgggg cttgtgaacc tgcgtccctc agaaaaccca tagaaaaata
     4141 gaaacccgaa tgtagtataa gaccatatgc aaagacctct ccatcaaacc tctttctctt
     4201 aagaagtata tacgttagca ccagtacaat tatgatatta cccagcgctg aataaagctg
     4261 tgtaggatgg agtgaaagca caccgagata gcgctcggga ataagcgtta cgccccttag
     4321 ctcctcggga taaataatcc cccagggaag tgaggtcggt tttccatagc agcaacctgc
     4381 agaaaaacac ccaagcctcc cgataccctg tcctaggatt gaagccggag cgattacatc
     4441 gagatacctc ttcatattga gtttggttct tttcatgtaa taaagagcag caagtaaacc
     4501 agcggcaaag ccaccgtaaa acacagctcc tccgccccct ttttcaaaaa gtgagtgtgg
     4561 atgttctaag aagtgttcaa atttgtagat aatatatagg accttggacc cgagtattcc
     4621 aaagaataca cccaagaggc tcatataaat catcctctct tcgctctccc ccaatcttct
     4681 tatcatgtaa actgcatatg aaagaccgat taaaatccca aggataaaca ttaccttgta
     4741 agtatggatt gttatcagat agccctcaga tggattacca aaaagcctga gtaggggata
     4801 catttattgt cacctacggg cgaagggatt cgagccacaa accttcgatc caggagacca
     4861 atgctctatc ctcaaatcta ataccgtttc cgacggaaaa gtgtttataa atgccgatac
     4921 caaactgtag gagcggcttc tagccgcgat ttagtcacac caaagatggt gctcctacta
     4981 gcaatatatg aacacttaac aaatggaaat gatataattg ccctttctca gattataatt
     5041 ttgcagtgct tttaatggac tgtaaagggg gtaggcgacg agtcttgctc gtcaccaact
     5101 aactttttgc acaggatgcc gctgtgcgtc agatcgcttc gctattactc gcgatgacaa
     5161 aaatggaatg tcattgcaag gagcattatt tcgagagcag tgttcgagaa atggaaataa
     5221 aagggaacca cgaagtaatc tagagggatt gcttcacctt cggttcgcaa tgacaacaag
     5281 gggaagtgtc attgcgagtg agtgaaacga ccaatgcaat ctaacattgg gtcagagggt
     5341 aatttccttg acatagatca tgctggtctg tagcatttgg aaagttttaa tcgagataat
     5401 atactctaaa cccaaattct cacatgggct cggaacagat acaaattgca ttgtcacgat
     5461 agccactttt aacccgctga ggtaaaccgg tcagggagca aatcagtgcc taagtctcag
     5521 gttttgctgt ttgccgtttt tctctttctt gggaaaccgg ctagcgaggc ggcatctctt
     5581 gagcctgttt tacctcagga tccggaatct agaccccagt taaaaccggc taaagaggaa
     5641 actccagagt ggatgcggcc ctggctccag gaaatcgggg cgatgattgg atacggctta
     5701 gcccacgaga tctttgggac tagggatgga aggaggcagt atctcatcct gaatccacgc
     5761 tgggggatgt tcctaaccgg cgtgataggt aaggttccct acaaaggcgc gctagagttc
     5821 ctcgttgaac caatagcaat gttccaattc gaaccgaata atcgttacgc tctgggattg
     5881 agtgcgctcc ttcggtataa cttctggaca ccctcgagga gggttacccc cttcttcgac
     5941 gctgggggcg gagtcatgtg gacgaatctc ggccttccag atcttgggtc aaattttaac
     6001 tttcagctcc aatttgggcc cggtctccac gtccacttca acagaggaac cgctttaacc
     6061 ttccagtatc gtttccacca catttccaac gccgaaaccg ctaagcccaa ctttagcatc
     6121 aactcaagcc tcctcctcat cggtgtcaca ttcttcctgt tatgagagaa gataataact
     6181 gcggggcaag caaagaacct gttgttcctc gaggcggaac tttctagtca cgctctcaaa
     6241 catagaatgg aaacctaaag gtttcctcta aaaaattttg cgtaggtctt gggaccaccc
     6301 accctaacct atccctccag tccatacaca ctcgaaggag gaaggatatg ggaggtcaat
     6361 atggaatcct taaggaatcc gctacatcca taacggggtt agaaaacccc ctctatatcg
     6421 tttactcatc taatgtctta agtgctaata gggtggtttg taatcttgat gaataattaa
     6481 aatgactaaa tcacgttacg catatcttac gctaggttcg acaggctcac ttcaggttgt
     6541 catacccgaa tgcttttatc gggtatccat aattaaaccc tggattcccg cttagaatat
     6601 gcgggaatga caaccttctt ccccttaagc gtatataagt gcgcaacgtc ggtgacttat
     6661 taaaagcact tgacaattaa ttgttatttt gagtgagtag aggtcaatca aaggaggtga
     6721 gtgatggaga agattaagag tgtgttttca attaaatggg taagatatat attccttctt
     6781 ttcctattat tgaccttggg atttcttatc cctatcgcgt gtgatggacg gctactctat
     6841 cccaaattgc tttagctttc tataaagggt atcccgtgag attccaagta tctcagacgc
     6901 ttttgattta ttcccttgag attcgcgaag cgcctgctct aacgcaaacc tctcgagctt
     6961 ctctagggaa agaagcgtgt gttcaagagg ctttgtttcc tgccatacta aatgtgcttt
     7021 taaatccttt tcggttattg tctccttttc cggcggtgaa atagctatta tgccctctat
     7081 taaattctct aattccctta tgttcccagg gaaatgatag cgataaagtg atgtgaatac
     7141 cccctctgat acccctttta tgctccttga atacctatcg ttaaacttcc ttataaaata
     7201 ttctgtgagt aatggaatat cctcccttct ctcccttagc ggtgggagtt ctattaccac
     7261 aaccgctagc ctaaagaaga ggtcctccct taaataggta ttctttagct cttcgattga
     7321 acgattgctt gcagctatta ttcttatgtc aacttgtatc tcgctcgtat accctattgg
     7381 tcttatcttg tgctcttcaa gaactcttag tagttttact tggagatccc ttggcatctc
     7441 tcctatctca tcaagaaaga tcgttcctct atttgctgcc ataaaaagcc catcatgatc
     7501 cttcatcgca ccagtaaatg accccttctt ataaccaaat aactcggatt ccataagttc
     7561 tttaggtatt gcagcgcagt ttactgcaac aaaaggttgg ttgctcctct cactctttag
     7621 atgaatggcc tttgccaaaa gctcttttcc tgttccagtt tctcctacaa taaatacaga
     7681 ggcatctgtg cgagcagcag cagtagctct ctcaaataca ccccccatta gccgagaact
     7741 gcttatgatc tgttcaaaac catacttttc agaaagaagc cctttaagct cagttatcct
     7801 gttctcaagc tcatgtgttt tttttattac ctctagcttc tttaacagta aatccttgtc
     7861 aaatggcttg gtgataaagt ccatagctcc acgttttatc gcctctacag cattaggtac
     7921 agtaccatga cctgtaataa taattgcatt tgtctctggg tgattttctt taacctcgtc
     7981 aagcaacgta aaaccatcca tcattggcat tacaaggtct aagagagccg cacaaatttg
     8041 gggaaaggaa gacatcttgt caatcgcctc ttttccagaa aaggcggtaa taatctcata
     8101 cccttttctt tcaagaagct cctttacagc atttactaat tcttcatcat catcaacaac
     8161 cagcacatta catttatgca accgttcttt cctcactata aatcggaagc cttattaaaa
     8221 cactagtccc ctcaccctct gtgctttcta tactaatctc cccattatgg tcttgaatta
     8281 tcctcttagc tacggaaaga cccaaacctg ttccctttcc agcaggctta gttgtaaaaa
     8341 agggatcaaa aataagtggg agtacgcttc tagaaatccc taccccggta tctgacattt
     8401 ttatctgtat ctgaccatcc ggattaacat tagaaatagt tatcgaaagc cttcccccac
     8461 tatgcattgc atccaccgca ttcatcatga tgtctgagaa aacaagcaga agtaaattac
     8521 tatcagccca gaccttgaca tcttcatcca agaataactc aagttcaacg ccttccttcc
     8581 taagaagagg ctttattata tctaccgcct cctcaataag ataagaaaca ttctcaggac
     8641 gctttacatt ttcaggtttg cttgcaaact taagaagcct ctgcataacc ctctgggcat
     8701 agaaaatctg cttttcaatt gaatcaagcc tttccataaa ctttttagac tcaggtattt
     8761 cgtcaatgtc ttctttaagc atctgaaccc ttagaagcat agaagcaaga ggagttccca
     8821 tatgatgagc tatcccttca gccaattttc caataacctg agctttttca agttctacta
     8881 ttttattcct aagtttgatt ctttccctta tatctcttat cgtttcaata aacattccac
     8941 ttccatcagt catatatact aaccctatac ttacagggat tctctctcca ttatctatta
     9001 ttttttctat ctcgtaatct aataatctgc ttccctcttt tacactctta aggatattag
     9061 aaaaatcggc tcggttttct agcggtataa catcccatat gtttttaagt cccgttttat
     9121 tattgctaca aaataacctt tctgcctctc tatttattgc aattaccatg ccatcttcat
     9181 cataaaccat gatcgcatca gtggtattat ccatgattgc ttgaagtctt tcctcacttt
     9241 cgcgaagcaa ctgttctgcc cgcttgcgtt cagttatatc cacaatagtt gccaggacta
     9301 agactcctcc ctccgtcttt acagggttga gtccaatctc aacgggaaac tcacctccat
     9361 ccttacgcat agcaaagagg tctcgacccg ttccgattag cctggcttgg ggattaacaa
     9421 agaagctttt acggtactca gaatgcttgg tttgaaaacg ctgaggcacc agaatctcta
     9481 cggtccgtcc gattagctct tccctattgt aaccgaacag cttctctgtc tgggagttga
     9541 caagaacgat ttcacccttc tgattcacca tcaatatggc gttaggagcc aactctacga
     9601 ttaggcgaaa tttctcctgt tctagaccgc cctccatttc agattcggca accccttggc
     9661 gcacttctac cacctcaaca acttgcttac ttatcttatc tttatctcgc atctcatgcc
     9721 tattaaagag ctatcttttt tggctccttg cgaggtttct tgtagactgt agaaaccttc
     9781 taatcacttt ctgcctaaag aaaatctgca ttccaattga ccaaggttcc tcagtccctt
     9841 ttatgagttt agcattcggt cactaaggtt acaaggtaga cctaaggtga taatcggtta
     9901 tttcccgcca tatattatgt tataaaattc gaaccttctt gcccaatcct taagattgta
     9961 ggatgcaatt agaaggtcct tctttatctc ggtctgctca tcctttatat agcaagtaag
    10021 tacagcttcg ggtttaaaac caaagtggtc taagattctt ataaaactct tattatcatt
    10081 agtaaatcgc actacaacct tttcaaagtt ttgatttagg ccctctgcta aaagcatctt
    10141 aaacatttga agaccgagcc cccttcctct atgttctggt tctacaatta atttaatctc
    10201 tgtagagaat ctccaataga gaccctcctt gtgaagtgta cccttaccta taattttttt
    10261 accccttaaa gcgattaatt gaaaaacctt atcatactta gagcttgtaa accagctttc
    10321 gacggtttct agtttaccca catcatcttt aaatattata agatcagacc tcggtatttt
    10381 actgaagaaa tccaataaac cctctttatc atcctcctca agaggtctga gtattattgt
    10441 tgttttatcc tttagtatta tttcccttgg gtatataatc atctcgttat gtttgaaatt
    10501 agcaaaatcc atgccatcat caactcaggg atattatcaa acttattcgg tcacaatggc
    10561 tttaattatt gtcagatgtc agatattctg acaaaatcat gtaaactaag tgcccaagaa
    10621 gtattgaaat ctaatggaaa atttttatgg tatggaattt gcgaatacat tttaatcatt
    10681 tgtttgtatc ttatgatttt tagggagtga aagtggcaat aaactctttt agttaagggg
    10741 gattcagtgg aaataagcaa aattctatgg gcatccgatg gttcaatgga atctgatatg
    10801 gctttacaat atgttgagtt gctagcaagt aagtacggat ctgagatagt cggtttaagc
    10861 gttgtccagc ccgttgatat agtaagattg aaggtaccgg tggatgtaaa aaaagagttg
    10921 ttctcaattc aggctttact agagaaaaaa gaatataaca gaataaataa ggcattaaaa
    10981 caacttgaac taaaagatac aaagttggac atatgtattg agaccggaat accgcataaa
    11041 gagattctaa gggtagctag ggaagaaaaa gtagatttaa ttgcgatggg aaagagaggg
    11101 ttagggctta aagatagaat tcttctcgga agtaatacaa ataaggtatt gaggatggca
    11161 aatgcacctg ttttagccgt aagacataag aagggagata aaattgatat aaagaagata
    11221 cttgttccaa cagacttatc agagcttacc tcaatttcat tagactttgc cattgatata
    11281 gctaatagat tcaattcaac aatatacctt cttcatacat tagagcttca tcacagctat
    11341 gagatttcaa caagtaaaat tatagaggaa
//
LOCUS       DATDRL010000098         5627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1111822, whole genome shotgun sequence.
ACCESSION   DATDRL010000098 DATDRL010000000
VERSION     DATDRL010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111822"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..1354
                     /gene="hemH"
                     /locus_tag="VI935_03335"
     CDS             395..1354
                     /gene="hemH"
                     /locus_tag="VI935_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HLE24665.1"
                     /translation="MPDKFDAILMIAFGGPEKPEDIRPFLDKVTLGRPIPKERIVEVA
                     HHYELIGGRSPLNELTFRQAHGLEADLKERGYELSVYVGMRNWHPLLTETVGEMADRG
                     VKRALGLIMAAHQCDASWQRYQRDVEEAISKTGVQLSVDYTAPLFDHPLFIEASADRI
                     YECLMQIPPTDRESAVLVFTAHSIPTPMAAESPYVKQLTTSSRLIAERLRFDLAHHGR
                     HDRWMLAYQSRSGRPTDPWLEPDICDVLRDLAKQGVRQVVVQPIGFLCEHVEVLYDIG
                     IEAVEVAKEVGITLLRAKTVNDDPKFIAALADVVERHIKDGTR"
     gene            1491..3059
                     /gene="hemG"
                     /locus_tag="VI935_03340"
     CDS             1491..3059
                     /gene="hemG"
                     /locus_tag="VI935_03340"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HLE24666.1"
                     /translation="MRIIVIGGGITGLSAAHHLVELHLQKNLPLEPILLEGSGRLGGA
                     ITTRHTDGFLMEEGPDSYITTKPWALSLCRRIGLEPKLIPTNYACRRTFVVHRGRLLP
                     IPDGFFMLAPTQFFPFVRSPLFSWRGKLRMALDLVLPRAPRQDDESLASFVSRRLGRE
                     ALERVAQPLISGIYTADPEKLSLRATIPSFLEMEEKHGSIIKAMLLEQRRSKRRQSED
                     FVRSSSESLGQNKESGARYGIFVSFKEGMQTLVDALLARLTEDEVFRPQHSLSSSVFQ
                     KEIKRTGNGNKTVQLNQRVKHVVRSFDFAQGSPQAGMANGGWRVFMEDGTWLDTDGII
                     IATPAYQTAALVERLDKSLASDLRKIEYASSVVINLAYRGEDISHPLDGFGFVVPMVE
                     KLTIIACSFSSVKFAGRAPEGSVLLRCFVGGAINPEAYELENSELINAAHKEISSLLG
                     IKAQPLFALVHRHPQSMPQYPVGHLEHIAQINAKVSKYRGLAFAGNAYNGVGIPDCIR
                     SGEAAAESILTINK"
     gene            3648..4325
                     /locus_tag="VI935_03345"
     CDS             3648..4325
                     /locus_tag="VI935_03345"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HLE24667.1"
                     /translation="MSIANTIVVSDIHLGSDMSRAGELISVLDSWTFERLILLGDIFD
                     DLNFKRLRRLHWDLLSYIRQISKRKEVVWVEGNHDEGLVEVIPYLLGVKACKEYLWED
                     NGKKYLAIHGHQFDDFIINNGTITEIACSIYRFAQRIDTKGFYISKFLKRKSKTYLRL
                     SKKVANDAIRYSLSKGAEFVFCGHTHHAMSVIIDGIHYFNTGCWTDIPSTYISIGEDG
                     VKVHELF"
     gene            4522..5523
                     /locus_tag="VI935_03350"
     CDS             4522..5523
                     /locus_tag="VI935_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HLE24668.1"
                     /translation="MRILFGVQGTGNGHISRAREILRYLPTYADVDILVSGTQYNVDL
                     GFEIRYKLSGLAFVLGKNGGIDYWNSVKDARLYNLINDIAKLPVEKYDLIISDFEPIT
                     AWASKLKGAKSVTLSHQASFLSPKTPRPDKKNYPVELIMRWYAPCSIPIGFHFERYDT
                     FIHTPVIRQEIREANVRNNGHYTVYIPFYGDDFLIKYLSKVDVKWEVFSNHYKGNPYT
                     QGNVTIYPVSNDGFVKSVTSCEGLLSGAGFETPAEALFLGKKLMVIPMRGQYEQECNA
                     EALQRLGVPVVKALDMNFDKILRDWIDSSNVYHADYQNHLPSIIEYMLEIPKERKTF"
BASE COUNT         1662 a         1175 c         1252 g         1538 t
ORIGIN      
        1 ccttcacagt ctgcagccca ttgtgcccaa cgataggcaa atctatcgcg tatctatcga
       61 gaaatcgccc cgcaccgaca ggaagcgccc gcgcggcact atcaccatcc ggaaacttca
      121 gcgccgccga agcatcctaa gtgccgccta acaactcgat ggagccgacc cggtcagcca
      181 gaggttgagt ttagcgcgat actagccctg gctggccggg cggctcatct cgaggccgtt
      241 atcgggtatc cataattaaa ccctggattc ccgctaaatc catgcgggaa tgacagcgtt
      301 ccgtggcact ttaaacacta ttcactttga aatggtataa tctccgacag tgtttaccac
      361 cgattaaatc gggggttgag aagaggtttt tttagtgcca gataaattcg atgctatcct
      421 tatgattgcc tttgggggac cagaaaagcc tgaggatatt cgaccgtttc ttgacaaagt
      481 aacgcttggg cgtccaattc caaaggagcg aattgtggaa gttgctcatc actacgaact
      541 tattggaggt agatcaccac ttaacgagtt aacattcaga caggcgcatg ggttagaagc
      601 agacctaaag gaacgcggct acgaactctc tgtttatgtc ggtatgcgta actggcaccc
      661 tctgttgacc gagactgtag gtgaaatggc agatagagga gtgaaacgag cgcttggctt
      721 aatcatggca gcgcaccagt gtgatgcgag ttggcaaagg tatcagagag atgttgaaga
      781 agctatttct aaaactggtg tgcaattgtc agttgactat acagcgccac tttttgatca
      841 tccacttttc atcgaagcct ctgccgaccg catttacgaa tgtcttatgc aaatcccacc
      901 taccgaccgt gaaagtgctg tgctggtttt taccgctcat agcattccta cgccgatggc
      961 tgctgaatcc ccttacgtta agcaacttac tacctcatca cgccttatag ccgagcgact
     1021 tcggttcgac cttgctcacc acgggcggca tgatcgctgg atgcttgcat accaaagtcg
     1081 cagtggccgc ccaaccgatc cttggcttga acctgacatt tgtgatgtac tccgtgacct
     1141 tgcaaagcaa ggtgtgagac aggtcgttgt tcaaccaatt ggtttcctgt gtgaacacgt
     1201 agaggttctt tatgatattg gtatcgaggc tgtcgaggtt gctaaagaag ttggcattac
     1261 cttacttcgt gcgaagacgg taaatgatga tcccaagttc atcgcagctt tagccgatgt
     1321 tgtagaaaga cacataaaag acgggacaag ataataatca ggatgcagga taaaagaacc
     1381 aagatgcatg taaaaatttt catcatgaat cgtgcatcgt gaatcatgta tcatgatcat
     1441 cctattcgtg aaaattcgtg cacatttgtg gctaaaccta aaataaaaca atgcgtatca
     1501 tagtcatcgg tggtggtatc acgggtttaa gcgctgctca ccacctcgtc gaattgcact
     1561 tgcagaaaaa tctaccacta gagccaattc tactcgaggg tagcgggcga ctcggtggcg
     1621 caatcacaac tcggcatacg gacggctttc ttatggagga aggtcccgac tcatacataa
     1681 caaccaagcc atgggcactt tctttatgta ggcgtatcgg gttagagccc aaacttattc
     1741 cgactaatta cgcatgccgt cgtacatttg ttgtccatcg aggaaggctg ctgcctatcc
     1801 ctgatggttt ctttatgctt gcacccactc agttttttcc atttgtgaga tcgccactgt
     1861 tttcctggcg cggaaaacta cgtatggctc ttgatttagt actaccacgt gccccaagac
     1921 aggacgacga aagccttgca tcttttgtct cccgccgact cgggcgcgag gcgttggagc
     1981 gcgttgccca gccgctgata agcggcatat acacagccga ccccgaaaaa ctgagtttgc
     2041 gagctaccat tccgagcttc cttgagatgg aggaaaagca cggaagcatt atcaaagcga
     2101 tgttacttga acaacgaaga tcaaagcgac ggcaaagcga ggactttgtt aggagttctt
     2161 cggaaagctt gggtcagaat aaggaaagcg gggcccggta tggcatcttt gtatcgttca
     2221 aagaaggaat gcaaacactt gtggatgcgc ttttagcacg tctgacagaa gacgaagtat
     2281 ttcgaccgca gcattctttg agcagcagtg tgtttcaaaa ggaaataaaa agaacgggga
     2341 atggaaataa aactgtgcag ctcaatcaac gcgtgaaaca tgtggttcga tccttcgact
     2401 ttgctcaggg ctcaccacag gcgggaatgg caaacggagg ttggagagtt ttcatggagg
     2461 acggcacttg gcttgatacc gatggaatca tcattgctac accggcatat caaactgcag
     2521 ctttagttga gagactcgac aaatcgttgg cttcagattt gaggaagatt gaatacgctt
     2581 cctccgtagt catcaacctc gcgtatcggg gagaggatat atcccatccc ctcgacggct
     2641 tcgggtttgt agtaccaatg gttgaaaaac ttactataat cgcctgctca ttcagcagtg
     2701 tgaagtttgc aggtcgtgcg ccagaaggaa gtgtattgct tcgctgcttc gtcgggggag
     2761 caataaatcc tgaagcctac gagcttgaaa attcagagtt gattaatgca gcacataaag
     2821 agataagtag cctcctaggt atcaaagctc agcctctatt tgcacttgtg catcgtcacc
     2881 cacaatccat gccacaatac cctgttggtc accttgaaca tatcgctcaa atcaatgcca
     2941 aagtaagtaa atatcgtggt cttgcgtttg caggtaatgc ttacaatgga gttggtattc
     3001 cggattgcat acgctcagga gaagcagcag cagaatccat cctaaccatt aacaaataga
     3061 ctctaaatgt taagtagttg aattaacgtg gtagagggag ccttatcatc ttcgaacatc
     3121 taatttggca atagacactt ggtccaccag tccctgccct gggcaaagtt gaagggttgc
     3181 tggtggacgg caacgagcaa gcctgtcccg agcttaagcc gagggaactc attgcctacc
     3241 catttagatt attacctatt taatagatca aatatcatta gactcactca tattcaggaa
     3301 acctaaaggt ttccgctacc tttctttgat tttaccttta ataccagaac cttttgaaat
     3361 ctattcttaa tggaataagt cagcagctcc taccaaaggc ttagcaccct aggaaagtat
     3421 tttatttccc ttcgtgatag aacaatctta attggttctt aacatatttt taatacaaaa
     3481 ctcataaaaa ctgattttaa taattacaag agagcttaat ggggaaagat tttacagtaa
     3541 ctttggagaa acagattaga tgagtattaa cttaattatt tttgcaacga accaaccaaa
     3601 aaagatgatt aaaataacct tcccgatcct tcatatctat atacgaaatg tctattgcta
     3661 atacgattgt tgtctcagat attcatcttg gctctgatat gagccgagcg ggagaattaa
     3721 taagcgttct tgactcctgg acctttgaga gattaatcct tcttggtgat atctttgatg
     3781 acctgaactt taaaagacta cgacgactcc attgggactt actgtcttat ataaggcaaa
     3841 tatcaaaacg taaagaggtt gtatgggttg aaggaaatca tgacgaaggc ctcgttgaag
     3901 tcatccccta tttgctgggt gtgaaagcat gtaaggaata tctatgggag gacaatggaa
     3961 aaaaatatct tgcaatacat ggccatcaat ttgatgattt tataatcaat aatggcacaa
     4021 ttactgaaat cgcttgctcg atttatcggt ttgcacagag gatagatact aaaggtttct
     4081 acataagtaa gtttctaaaa agaaaatcta agacatattt gcgattatcc aaaaaggtag
     4141 ctaatgatgc tattcgttat agtctgagta agggggctga gtttgttttt tgtggtcata
     4201 cccatcatgc aatgtccgtg attattgatg gtatccacta ctttaacacc ggatgctgga
     4261 cagatatacc ttctacctac atatcaatag gagaagatgg agttaaggtc cacgagctat
     4321 tttgacatta gatttattaa acagttaatc atctaaaagt gtagacaata ggtagggacg
     4381 cattgcgatg cgtctctaca ataggttgcc gtctaccggc aaccattcga ctttgctcag
     4441 ggcagggaaa tggtagacta caaagtgttt atcacagaat taaatgttta aagatcataa
     4501 tggcttctgt gcgaggtgat attgagaata ttattcggtg ttcagggtac gggtaatggg
     4561 catataagcc gagcacgaga aattttaagg tatttaccaa cgtacgctga tgtagatatt
     4621 cttgttagcg ggacgcagta taatgttgat ctcggattcg aaataagata taaactctct
     4681 ggacttgcat ttgtattagg taagaacgga gggattgatt actggaactc agtaaaagat
     4741 gcaaggctct ataatttaat aaatgacata gctaaactac ctgttgagaa atatgattta
     4801 ataataagcg attttgaacc tataacagca tgggcatcaa aattaaaggg tgctaaaagc
     4861 gtaaccttat cccatcaggc atcatttctc tcccccaaaa cacccagacc agataaaaag
     4921 aattacccgg tggagttaat aatgaggtgg tatgcccctt gttcgatccc tataggtttt
     4981 cactttgaaa gatatgacac ttttatccat acgcctgtta taagacagga gatacgtgaa
     5041 gcaaatgtta ggaataatgg ccactacaca gtttacatac ccttctatgg agatgatttt
     5101 ttaattaaat atctgtcaaa agtagatgta aaatgggaag tattctcgaa ccactataag
     5161 ggaaaccctt acacacaagg caacgtaacc atatacccag taagcaatga tggttttgta
     5221 aaaagtgtta cctcatgtga agggctgctc agtggtgctg gttttgaaac accggcggag
     5281 gctttatttc ttggcaagaa actcatggta attcctatga gggggcaata cgagcaggaa
     5341 tgcaatgccg aagcactgca gagattagga gttccagttg ttaaagctct agatatgaat
     5401 tttgataaaa ttcttagaga ttggattgat tcatcaaatg tttatcacgc agactaccaa
     5461 aaccatttac catctataat tgagtacatg ttagagattc ctaaagaaag aaaaactttt
     5521 tgataggtta acttttttat tccctctcct tctttaaaaa tcccaagaaa aaaattctaa
     5581 atataaatga acaaccctgc tgcaatcaca tattcaaaac cataatg
//
LOCUS       DATDRL010000099         2196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_319832, whole genome shotgun sequence.
ACCESSION   DATDRL010000099 DATDRL010000000
VERSION     DATDRL010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319832"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VI935_03355"
     CDS             complement(<1..865)
                     /locus_tag="VI935_03355"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HLE24669.1"
                     /translation="MKLPLGWKINLGFGFALLILSIISLFSYISTTELIRTINTNQQV
                     QKVSENLEYAFSMLKDAESSQRGYTITGEESYLETYKVAIVSIDQAIKNLRELTADSP
                     DGRQRLSTLEPMISEKLELVKERIELRKNEGFEAAAQAIKTGEGKRTMDEIRKVITEW
                     ENKQNVLLKHQSKAMQASVQNAKSIIVFGSFIAFILAALTIFILNSDITERKRSEEEL
                     RLLNTITDAVHKSSDLKEVFNIALDKVMELTDIDIAGIYLVDEATNEAVLEAHRGFPD
                     KYVERAGRIPYP"
     gene            1624..>2196
                     /locus_tag="VI935_03360"
     CDS             1624..>2196
                     /locus_tag="VI935_03360"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HLE24670.1"
                     /translation="MEFGHMLLVLVLGISVISLGFAGYLAKYVLNSDTGTSAMQDISN
                     AIKEGAEAFLKRQYSTIIMLSIVLAVLIFVLYWFAKGDHDMAWRTTVAFLFGAGCSAL
                     AGYIGMWISIRSNIRTASAVRSSLDRALKISLRGGAVSGITIVAMSLLGVGILFTLYG
                     GSADDPEVVKRVPFLIVGYAFGASFVALFAQ"
BASE COUNT          608 a          443 c          454 g          691 t
ORIGIN      
        1 taggatatgg tattcttcct gctctttcta cgtatttgtc agggaatcct ctatgagcct
       61 cgagtaccgc ttcatttgta gcttcatcta ccaaatatat tcctgcaata tctatatccg
      121 ttagttccat aaccttgtct agtgcgatat taaaaacctc tttaaggtct gatgacttat
      181 gaaccgcatc cgtaatagta tttagtaacc tcagctcttc ctctgaccgc ttgcgctcgg
      241 tgatgtcact atttaggata aatattgtca aagcggcgag tatgaaagct ataaagcttc
      301 caaaaactat tatagacttc gcgttttgaa cactcgcttg catcgccttc gactgatgtt
      361 tcaacaacac attctgctta ttctcccact cagtaattac cttccggatt tcatccatgg
      421 tcctcttccc ctcacccgtc ttgatcgcct gagccgcagc ctcaaatccc tcgttctttc
      481 gcaactcgat cctctccttc actaattcga gtttttccga aatcatgggc tcaagggtgc
      541 tcagtctctg ccgtccgtcg gggctatcag cggttaattc cctaaggttc ttgattgctt
      601 gatcaattga cacgattgca actttataag tttccagata actctcttct ccagttatgg
      661 tatagccgcg ttgactggac tcggcgtctt ttaacatgga gaatgcatac tcgaggtttt
      721 cggaaacctt ttggacttgt tgattcgtat taatagtcct aatgagttca gtcgtgctta
      781 tgtatgagaa caagctgata atgcttagaa ttaacaacgc aaatccaaac cctagattaa
      841 tcttccatcc aagaggcaat ttcatattag tacactaaat tataattatg ctttctaagt
      901 gatacaacat tttcaatata tttaactata ctttaactat acttaaatcg gaacggctgg
      961 tggtagtatt aaggaaaggt tgagcaggga gctgagattc cgtaggtata tattatcatc
     1021 ccaaacggaa gtgagaggtt tattatacca aggtcaataa aaaagatgaa tttctcgttt
     1081 cacaaatgac aagagggcta ttgattccat tgtatagcca gttagttcct tcgattggat
     1141 tcaggagggg cttgttgttg cataatcatt ttgcgagcaa gactcgttgg ttaccctggt
     1201 gtgggagaag ggaattaaaa atggtaggac accactctcc taggctctac tcaatataga
     1261 cttagtagtg gccagttatg atttgtacat aatgataatt taatgagtgc tttaaaatcg
     1321 aacgatataa atgaattact gttttaagtt caaacaagaa tagattattt gagaagggag
     1381 attaataaag gaatcgcact tgtaatttat gagtttgcca gttatgattc ctttccctaa
     1441 aaaccgagtt gggactttcc aataggaata gggcttgaat ttgtttccat atagactgag
     1501 gataaagagg aaaactatgc ttgcaggtta ggtctaactt cagttgctag catactaatt
     1561 tcctaacatc ctaacaaaat taggcctaca atactttaaa taatagaaag gaggatataa
     1621 aaaatggaat ttggccacat gctccttgtg ctagttctgg gcatcagtgt tatatcacta
     1681 ggtttcgctg ggtatctagc caagtatgtg cttaacagtg atacaggaac ttcggcgatg
     1741 caggacataa gcaacgctat taaagaggga gccgaagcgt ttctaaaaag acagtactca
     1801 accataataa tgctaagcat cgttcttgcc gttcttatct ttgttctata ttggtttgca
     1861 aaaggagatc atgacatggc atggcgcact accgtagcct ttcttttcgg tgcggggtgc
     1921 tctgcacttg caggatacat tggaatgtgg atttcaatca gatcaaacat tcgaacggca
     1981 agcgctgtaa ggtcaagcct ggatagggct cttaaaatat cccttcgagg gggagcagtt
     2041 tcaggaatta caatcgttgc tatgagtctt cttggggtag gaatcctttt taccctctac
     2101 ggtggctcag cagatgaccc ggaggttgtg aaaagggtcc cctttctcat agtcggatat
     2161 gcatttgggg caagtttcgt tgcccttttc gctcag
//
LOCUS       DATDRL010000100         3395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3373269, whole genome shotgun sequence.
ACCESSION   DATDRL010000100 DATDRL010000000
VERSION     DATDRL010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3373269"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..310)
                     /locus_tag="VI935_03365"
     CDS             complement(131..310)
                     /locus_tag="VI935_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HLE24671.1"
                     /translation="MDNLSYLFWAHVIFWILLFLYILHLVEKNKKLQKELEALKNSIN
                     KTKEEKLTWQKQGVN"
     gene            complement(311..958)
                     /gene="ccsA"
                     /locus_tag="VI935_03370"
     CDS             complement(311..958)
                     /gene="ccsA"
                     /locus_tag="VI935_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HLE24672.1"
                     /translation="MGLTFVGMIASLYMAFIYAPTEMVMGNIQRIFYFHMGSVWVATI
                     AFTVVFIASIQYLRKQTRFWDIMAYSSAELGVLFTTLTIITGSIWAKPVWGTWWTWDP
                     QLTTTFILWILYIVYLILRSSAGSDVKKAKFAAVFGIIAFIDLPLVYASARLMRGISP
                     VVFGDRGGGIAPQMMVALVVCIITFTLLYIVLLKQRMDIEEVKDKVTRLKLEEAL"
     gene            complement(1085..1420)
                     /locus_tag="VI935_03375"
     CDS             complement(1085..1420)
                     /locus_tag="VI935_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLE24673.1"
                     /translation="MKYKFILMIGIWLVVISFGMRYAYGEEELVEKGKQLYTQKKCGL
                     CHTVDGSGGKKGGDLSDVGSKRDAEWLTKYMKDPKSVIPEAKMPAFRGTDEELNALVA
                     YLMSLKKAK"
     gene            1835..>3395
                     /gene="feoB"
                     /locus_tag="VI935_03380"
     CDS             1835..>3395
                     /gene="feoB"
                     /locus_tag="VI935_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HLE24674.1"
                     /translation="MHNHTNVNPSPHLKKAVLIGNPNIGKSLIFGCLTGRYVTVSNYP
                     GTTVEITLGDMKIGSEIISLIDTPGINSLTPISEDERVVRDILLKENITSVIQVVDAK
                     NLERGLYITTQTAEMGLPTLIVLNMMDEAQTRGVDINIAELSRELGIPIVPTVATQRK
                     GIERIKKELPNSSIPLIRISYSQTVEESLEEIEKIIPNPHFSRFIGLSILSGDGSLNE
                     DLISRVSKDKLSSLAQLHHFSQISSKEYPISYFIGLKRLELAKKITQVVSATSFKSES
                     RILETLGRISIHPVFGIPILVSVLYFAWLFVGKLGAGVFVDFLEGTIFEGYINPFFKI
                     IIPKIIPINLLSDFLVGDFGVITMALTYSLAIILPVICTFFIFFSLLEDSGYLPRLAI
                     MVDRVFKLMGLNGKVVLPMVLGLGCDTMATLTTRMLETKKERLIVTFLLALGIPCSAQ
                     LAVVFAMASTLPPWGILMWGGEITTLLFIVGFLASCLVKGESSDLILEVPPIRIPQIG
                     NVIIKTLARLEW"
BASE COUNT         1049 a          725 c          572 g         1049 t
ORIGIN      
        1 tccttaagct cctcaactgt aagataataa accatcgtat ctttcactcc accataaaca
       61 aggtaggcaa gcgtaagtgc gatagctgca atagcaatga taaacttaat cttccctcga
      121 ctcataacta ttaatttact ccctgctttt gccaggtgag cttttcctct ttagttttat
      181 taatagaatt tttaagtgct tccaattcct tttgtaattt tttatttttt tctactagat
      241 gtagtatata aagaaatagc aaaatccaga aaatcacatg agcccaaaat agatatgaca
      301 aattatccac ttataatgcc tcctcgagct tcagcctagt taccttgtct ttcacctctt
      361 caatatccat cctttgttta agaagcacta tatataaaag tgtaaaagta attatgcaaa
      421 ctaccagtgc aaccatcatc tggggtgcaa tgccaccacc tcgatcgccg aaaaccaccg
      481 gtgatattcc acgcatgagt cttgctgagg cataaacaag aggaagatct ataaaagcaa
      541 ttattccaaa taccgctgcg aattttgctt tttttacatc gctgccggcg gaagagcgca
      601 gtatcagata tacaatatag agaatccata aaataaaagt agtcgtgagc tgtgggtccc
      661 atgtccacca tgtgccccat acaggctttg cccatatact tcctgtgata atcgtcagtg
      721 ttgtaaatag aactcctaac tcggcagaag agtaagccat aatatcccag aaacgcgttt
      781 gcttgcgtag atactgaatg cttgctatga acacgactgt aaacgcaatt gtagcaaccc
      841 atactgaacc catgtggaaa taaaaaatcc tctgaatatt tcccatcacc atttcggttg
      901 gagcatatat aaaagccata taaagagacg ctatcatacc aacaaatgtt agacccataa
      961 gaagcttcat cgtttttgat aaaccattca catattttat tcctctacca catacttaaa
     1021 aacaatagaa caaagaatag cacaattacc cagatttttt catggggagg aagccacaat
     1081 tagcctactt tgcctttttg agactcatta aataagctac taaagcattt agttcttcat
     1141 ccgttcctct aaaagcaggc atttttgctt caggtataac ggattttgga tccttcatat
     1201 actttgttaa ccactcagca tctcttttac tacctacatc agagaggtct cctcccttct
     1261 tacctccact tccatccaca gtatgacata aaccgcactt tttttgagtg tacaactgct
     1321 ttcccttttc aactagttcc tcttctccat aggcatacct cataccaaaa gaaattacaa
     1381 ccaaccaaat tccaatcata agaatgaatt tatatttcat cacagtgcct ccttaaaaaa
     1441 ttgtgtgaaa tattataaca cagattttta tcatcgtaag ttaaaacgta atttttaaac
     1501 tgcaaagtct tactagttat aacatggcat cccatagttt gtcgcgtatc caccaattta
     1561 atcatttctt tagatttata tttgcaaaac ttatgccaag caattcatgt tgaacttcct
     1621 tgtaaaacaa aaggtaaaat attaaattct ttgtcctatt tcctgacatc tgacagtcga
     1681 atgatgattt gttagctttt tcttaatctt tttaaactta aaaaattcat atagaactta
     1741 tagttagctt aagccatatt cgctttctta ctcttggcat catacttgcc atattgaaaa
     1801 tgagattcaa tttctaatat gagacggact ctatatgcat aaccacacca atgttaatcc
     1861 ctcacctcac ctaaagaaag cggttttaat tggaaacccc aatatcggga aaagcttaat
     1921 atttggttgt cttaccggga gatatgttac agtctcaaat tatcccggga caacggttga
     1981 gataacccta ggggatatga aaatcggctc tgagataatt tctcttattg atacccctgg
     2041 gattaatagc ctaacaccga tatcggaaga cgaaagggtt gtaagagaca tactgttaaa
     2101 ggaaaacata acctctgtaa tccaggttgt agacgctaaa aatttagaaa gagggttata
     2161 catcacaacc cagactgcag aaatgggtct tcccactctt atagtcctaa atatgatgga
     2221 tgaagcacag acaagaggag ttgacattaa tatcgcagag ctctctaggg aacttggaat
     2281 tccaatagtt ccaactgttg ctactcaaag aaaagggata gaaagaatta agaaagaact
     2341 cccaaattca agtattcctc taattaggat ttcttactct caaactgtgg aggaatcttt
     2401 agaagaaatt gaaaagatta taccaaaccc tcacttctcc aggtttatcg gtcttagtat
     2461 tctatcaggg gacgggagcc taaacgagga tcttataagt agagtttcaa aagataagct
     2521 tagctccctt gctcagcttc accacttctc acagattagc tcgaaggaat accccatcag
     2581 ttattttatc ggcctaaaaa ggcttgagct tgcaaagaag atcactcaag tagtatctgc
     2641 cacctcattt aaatccgaaa gccgcatcct agagacctta ggacggattt caattcatcc
     2701 cgtatttgga attcctatat tggtttcagt actttacttt gcatggctct ttgtaggaaa
     2761 gctaggagcg ggggtctttg ttgatttctt agagggaacc attttcgaag gatatataaa
     2821 cccctttttt aaaataatta taccaaaaat tatccctatt aacctcttga gcgattttct
     2881 cgtaggtgac tttggagtaa ttactatggc acttacctac tcacttgcca taattctccc
     2941 tgtaatctgc acctttttta ttttctttag tctccttgaa gattctggat acctgccaag
     3001 gctggctatt atggttgaca gggtatttaa gttaatggga cttaacggaa aagtcgtgct
     3061 tccaatggtt ctggggctcg gatgtgacac aatggctacc cttaccacaa ggatgcttga
     3121 gacaaaaaaa gaaaggctta tcgtcacatt ccttctggcc ttgggaatcc catgctcggc
     3181 tcaactggcg gttgtttttg ctatggcttc tactctgcct ccgtggggta tcctcatgtg
     3241 gggtggagaa ataacaaccc ttcttttcat agtcggtttt cttgcctctt gccttgtaaa
     3301 aggagaaagc agtgacctta ttcttgaggt tccgcctata aggattcctc aaatcggaaa
     3361 cgtaattatt aaaacccttg ccagacttga atggt
//
LOCUS       DATDRL010000101         3141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2491552, whole genome shotgun sequence.
ACCESSION   DATDRL010000101 DATDRL010000000
VERSION     DATDRL010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2491552"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VI935_03385"
     CDS             <1..390
                     /locus_tag="VI935_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24675.1"
                     /translation="ACPEPNLDIKVRDPETGGPFSLDIDNDSFPVVPESESVLESEDG
                     TIRLEITVLEETEEAAGIDTRVLVEEEFENGELIEVSRNFFAQAPDGTVCYFGEDVDE
                     CADGLEQVGYEFLCNGEEPSHEGALRA"
     gene            complement(443..916)
                     /locus_tag="VI935_03390"
     CDS             complement(443..916)
                     /locus_tag="VI935_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24676.1"
                     /translation="MERIKPIRIIAAGILGGMAASLAVLVLFRLIGFEYWGILTNPEY
                     QSPKLLKVWTEIEPIPLAVNHPGIITLGFVILSILHAFVFAIIRQGIPGEGLKKGIIF
                     GLIIWLFSYVFFEFFTPWNMFGEPLYLVGLELLFWIGVALSEGIVIAKVYNVDSM"
     gene            complement(998..1336)
                     /locus_tag="VI935_03395"
     CDS             complement(998..1336)
                     /locus_tag="VI935_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24677.1"
                     /translation="MKKHSKNTNQHFEKLLARFSKLPEVEKKRMFGYESFFTHGRCFG
                     GIRPLGVETSVVLRLSHSDYTKAVKSPSLFSPFRNTKDWVECKATSLKDVIKLMPWIE
                     LSYFYARRGI"
     gene            complement(1520..2383)
                     /locus_tag="VI935_03400"
     CDS             complement(1520..2383)
                     /locus_tag="VI935_03400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLE24678.1"
                     /translation="MKVKDKAGFTNKVVIITGASSGIGRATALAFAREGAITILASRS
                     LENLQKVADGIRNFNPNVLVISTDVSLQNSVNNMVEKVMARYGRVDILFNNAGSAFVG
                     PIDHEKFVEDIKKMIEVDFFGTIYCTKEVLPIMKKQGSGHIINMSSVVGRKAFPHFGG
                     YSATMHAISGFTDALRQELYGSGIDVSIIHPALTQTPLLEHVKPEEIPPPFKRFTPIS
                     AESVAEAVLRGVQKKEYRIFVPFQPKMLLLADAISPRLGDLIVRLMPNRVFASLIGIY
                     SGKVYEHNKSY"
     gene            complement(2432..2884)
                     /locus_tag="VI935_03405"
     CDS             complement(2432..2884)
                     /locus_tag="VI935_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HLE24679.1"
                     /translation="MSHEIDMSELTGCANCACFNLRKAARAVTQLYDNILRPVRLRAT
                     QFSLLVATSLFGPISVTRLAEMGVIDRTTLTRNFKPLVKRGYIEVALGKDRRTRVVSL
                     TGRGREVLRKALPLWKKAQARVVKELGQERWSSLQANLQEVVSLASKS"
BASE COUNT          888 a          698 c          599 g          956 t
ORIGIN      
        1 gcatgcccag agcccaatct ggacattaaa gttcgcgacc cggaaaccgg cggcccattc
       61 tcactagaca tcgacaacga ttccttccct gtggttccgg aaagtgagag cgtgcttgag
      121 agtgaggacg gaacgatccg attggaaatt actgttcttg aagagacaga agaggccgca
      181 ggaatagata cacgcgtcct tgttgaggag gaattcgaga acggtgaact cattgaggtc
      241 tcccggaact tcttcgcgca ggcgccagac ggcactgtct gctacttcgg cgaggacgtc
      301 gatgaatgcg ctgatgggct tgaacaggtt gggtatgagt ttctttgtaa cggtgaagag
      361 ccgagccacg aaggtgcatt gcgtgcttaa aattaataat aaaggggtca gaccccttta
      421 ttattccgct acagttatat acttacatcg aatccacatt ataaaccttt gctattacaa
      481 ttccctcaga gagagccact cctatccaaa agaggagctc aagtcccaca agatacaacg
      541 gttctccaaa catattccaa ggtgtgaaga actcaaagaa tacataacta aatagccata
      601 ttattagccc aaagattatc cctttcttca agccctcgcc tggaattcct tgtcgtatta
      661 tggcaaatac aaaagcatgg agaatggaaa gaataacgaa tccgagggta atgatgccag
      721 gatgattaac ggcaagggga ataggctcaa tctcagtcca taccttaagt agttttggac
      781 tttggtattc ggggtttgta agaattcccc agtactcaaa tccaataagc ctaaatagta
      841 ctaaaaccgc taaacttgct gccataccac ctaagatgcc agcagcgatt attctaatgg
      901 gtttaatccg ttccatattg tttcctcctt gaataatttg ttattagtcg tttgtcgtta
      961 atcgttcgac taaagactaa ggactattaa ctatcaacta tattcccctg cgagcataaa
     1021 agtaggaaag ttcaatccaa ggcataagct taataacatc ctttagtgaa gtcgctttgc
     1081 actcgaccca atcctttgta tttcggaaag gggaaaacag agatgggctt ttgactgctt
     1141 ttgtgtaatc gctgtgggat agcctaagca cgactgaagt ttcaacaccc aatggtctaa
     1201 ttcctccgaa acatctacca tgtgtaaaaa agctttcata tccaaacatg cgttttttct
     1261 ctacctccgg caatttagaa aatcttgcta aaagtttctc aaaatgttgg ttcgtattct
     1321 tactatgttt tttcatgaac attcctccct aatgcatgat tcatgatgca tgatgcgcta
     1381 ttcatgaatt tttttacttt catcctgtat ccagcatcgt cagactgagc tcacgactaa
     1441 gtcttgcatt gagcatcgtt catcctgctt attttcattc gtgcttattt gtgtcaattc
     1501 gtggctaatg tttaccgaat cagtaagatt tattatgttc atatactttt ccactataaa
     1561 tcccaatcaa agaagcgaac accctattcg gcatcaacct tacaatcagg tctccaagtc
     1621 gtggagatat agcatcggcc agaagcaaca ttttgggttg aaatggtacg aaaattctat
     1681 attccttttt ttgtactcct cttaacacag cttcggcaac ggattctgca gatattggtg
     1741 taaatctctt gaacggagga ggaatctcct caggtttaac atgttcaagt agaggtgttt
     1801 gagttaatgc cgggtgaata attgaaacat ctattccact tccatatagt tcctgcctta
     1861 gcgcgtcggt aaatccagag atcgcatgca tggtggctga gtatccacca aaatgtggaa
     1921 atgccttcct gccaacaacc gaagacatgt taatgatatg accagagcct tgctttttca
     1981 ttataggtaa gacttccttc gtgcaatata tagttccaaa gaaatcaacc tcaatcatct
     2041 ttttaatgtc ttcaacaaat ttttcatgat ctatcggtcc tacaaaagca cttccggcat
     2101 tattaaaaag aatatctacc ctcccgtacc gtgccataac tttttctacc atgttattga
     2161 cgctgttctg taatgaaaca tcagtggaaa taactaatac attgggatta aagttgcgta
     2221 ttccatccgc cactttttgg agattctcta gtgaacgtga agccaggatt gttattgcac
     2281 cctcacgggc aaatgccaag gcagtggcac gaccaattcc gcttgacgct cctgtgatga
     2341 ttactacctt atttgtaaat ccggctttgt ctttcacttt catgtttaat cctccttttt
     2401 ggtgtatata cacctttata atcaaaaaaa tttaactctt acttgctagc gatacaactt
     2461 cctgcagatt ggcctgtaga gaactccatc gctcctgacc taattctttt acaacacggg
     2521 cctgagcctt tttccagaga ggaagtgcct ttcttaaaac ttctctacca cgcccagtca
     2581 agcttacgac tcgagttcga cggtctttac ctagtgcaac ctctatgtat ccccgtttta
     2641 caaggggttt aaaattgcgg gttagtgtag tcctgtcaat tacgcccatt tcggcaagac
     2701 gcgttacaga aataggccca aaaaggctag ttgccacaag aagcgaaaac tgtgtagcac
     2761 gaagtctaac tggcctaagt atattatcat aaagctgagt aactgcacgg gcggcttttc
     2821 gtaggttaaa acatgcacag tttgcacacc cggttaattc tgacatatct atctcatgag
     2881 acattggctt aactcctcta attcttcatg tctgaaacta cctatttttg tttcttgtaa
     2941 taggagtata tacacctatt ttatatttgt caagtacttt ttttaatttt ttaaatttaa
     3001 ggtgtcataa ctatttagaa atgtcctcta gtgtatctag aatttccact ctggtacgca
     3061 ttggtattta gataccatta catctttccc aatctgaatc aagctcgttt cctcttcttc
     3121 caagaacaac gccatgggtg a
//
LOCUS       DATDRL010000102         6036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2143778, whole genome shotgun sequence.
ACCESSION   DATDRL010000102 DATDRL010000000
VERSION     DATDRL010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2143778"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VI935_03410"
     CDS             complement(<1..275)
                     /locus_tag="VI935_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24680.1"
                     /translation="MERNLHYKKAYSEGAEKGLGNPSDFRNEEEQRAEGLLDAYSKAV
                     ITVAERLSPTVVSIKTTQEVKAQTPRGIVPFDATASGSGVIITPDGY"
     gene            complement(522..860)
                     /gene="trxA"
                     /locus_tag="VI935_03415"
     CDS             complement(522..860)
                     /gene="trxA"
                     /locus_tag="VI935_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HLE24681.1"
                     /translation="MSVSILLTDENFEVEVTSSNVPVLVDFWAEWCGPCRKVGPIIEE
                     IAEEYSGKLKVGKLNVDENQISTYSYGIRSIPTLIVFKDGKPVDKIIGAFPKRHIIGV
                     IEGALNGDHS"
     gene            1115..2737
                     /locus_tag="VI935_03420"
     CDS             1115..2737
                     /locus_tag="VI935_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HLE24682.1"
                     /translation="MQNDFLSNKYQRIIESIAEAILEIEDGAFSVSKAKIPEFINNYL
                     SSMSPDLKQRAKILLSLFRYSPFLYMKLKTFPSLGLEKRQKILRDFMKSNLRPKLLVF
                     QTLKTLTVMGYYRNEETWKDIGYDGPTIKQFPPTEPIILERGEKLKTAEDISTDIREK
                     ADVCVVGSGAGGAVTAKELSQKGLNVILLEMGGYNTSRDFNQREEDMYPLLLAEQGAS
                     STGDYSINVIHGKGVGGSTVHNTGLCYRTPKEILELWEREWKVEGLTFNELDPFYNDV
                     EKTLSIQPIRIEEVNPNNAIALEGAKKLGLSTILPNHNRSGECPGCGFCILGCSYDKK
                     QSMLVTYIPLAYKYGTKIYADCMAEKIELKNGRAKGVYGRFYRNGIPTHPIYIEADTV
                     VVSGGAVNTPGLLLRSGIDSGGKVGQNLHLHPFIFVGGIFDEKIEGWKGIPQSVVVDE
                     FLKQNGKGLKGYLIMPGFAHPILFASLTPSFGKEHRRIMKLYPQFATLGVLLHDSSSG
                     KIYVNKNGRLRISYKLNKDDKRELTHGMKKCA"
     gene            2789..3070
                     /locus_tag="VI935_03425"
     CDS             2789..3070
                     /locus_tag="VI935_03425"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLE24683.1"
                     /translation="MFIKSKKDIATINQLKIIKNRTHIISVHPQSSCPMGEDKKKSVV
                     DSFCRFHGIENLYISDASVFPTSIGIPPQITIMALAARTAKYIAKQMGR"
     gene            complement(3368..5479)
                     /locus_tag="VI935_03430"
     CDS             complement(3368..5479)
                     /locus_tag="VI935_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HLE24684.1"
                     /translation="MEKPNRLINEKSPYLLQHAYNPVDWYPWGAEAFEKAKRENKPIF
                     LSIGYSTCHWCHVMERESFEDPEVAKLMNEVFVSIKVDREERPDIDSIYMTVCQMMTG
                     SGGWPLTIIMTPDKKPFFAGTYFPKESRFGRIGMTQLVPRIKEIWTTKRDEIVNSANQ
                     ITSALQQATKESLGDKLDEFTLRVAYNQLAERFDTMHGGFGRAPKFPTPHNLLFLLRY
                     WKRASNEDALEMVENTLQAMRLGGIYDHVGFGFHRYSTDAEWFLPHFEKMLYDQALLA
                     MAYIEAYQVTGKEEYKKAAREIFTYVLRDMTSPQGGFYSAEDADSEGEEGKFYLWTEE
                     EIRAVLGKEEADLTIDVFNVEEGGNFSEEATGRSTARNILHLKKSIPAIASQLKISEQ
                     ELEQRLEAAREKLFSVREKRIHPHKDDKILTDWNGLMIAALAKGAQVLNEPKYGEAAK
                     RAVDFILKNLRKPDGRLLHRYRDGQAAIPAHVDDYAFFTWGLLELYEATFDVNYLQTS
                     IDLNRDLITHFWDDINGGFYFTADDGENLLLRNKEIYDGAIPSGNSAAMLNLLRLDRI
                     TTNSNFEEMAEKIGSAFSKTIKQFPAAHTELMVALDFGVGPSYEVVIVGDSQADDTKA
                     MIKAIRRQFVPNKVVLLRQTEKEEPDIIRIAEFTKSQLSLEGKATAYVCLNYVCKLPT
                     TDISKMLEFLSGKKQENTKAF"
     gene            complement(5689..>6036)
                     /locus_tag="VI935_03435"
     CDS             complement(5689..>6036)
                     /locus_tag="VI935_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24685.1"
                     /translation="DSERRIQDIIKARDGAVFSLISLPNGLTLNTDEYLHFEVLVTKE
                     KRDVRYINNLKSPINFPTEPPSLESSVEMQTQMPVRTHPVRLQGFPESVFIIVAKHKG
                     ILSNEAVITSPLD"
BASE COUNT         1789 a         1301 c         1198 g         1748 t
ORIGIN      
        1 atatatccat ctggtgtgat aataaccccc gagccgctcg cggtcgcatc aaagggaact
       61 atacctctag gggtctgagc ctttacctct tgggttgttt taatactgac aacagtaggg
      121 cttaatcttt cagccacggt gataaccgct ttagaatagg catcaagtaa accctcagcc
      181 ctttgctctt cttcatttct aaaatccgag gggtttccta aacctttctc tgcaccctcg
      241 ctgtatgctt ttttataatg tagattcctt tccatatttt gctccctaat cctaaattaa
      301 tacataaatc aggattttcc agctatggta ttctgcaaaa caatccttga ttgattgtta
      361 atcattaaag aacgactctc ctcgcctgtc attgcgagcg ttcgcgaagc aatctcaaaa
      421 acaatcagga gattgcttcg gtatcttttt attaccattt ctcgcacact gctctcgaaa
      481 taatacgcct cgcaatgaca atttcagagc aattcaagcg gttaactatg atcgccattt
      541 agagcaccct caatgacccc tataatatgt cttttaggaa aagctccgat tattttgtct
      601 acgggcttgc cgtctttgaa gacaataagg gtaggaatgc tccttatacc ataactatat
      661 gttgatatct ggttttcatc tacgtttagt tttccaacct ttaatttgcc gctatattcc
      721 tctgcaatct cttcgataat aggaccgact ttcctacaag gtccgcacca ttcagcccaa
      781 aaatctacta gcactggaac attagaactg gttacttcaa cctcgaaatt ttcatctgta
      841 agtaatattg aaacgctcat tattgtaacc tccattacgt atctatgtgt atttacacct
      901 tttagatgag agtatatgaa aaaagattca aatctgccat atgagcgaat gaggacaaat
      961 gtaggacacc atctcagaga ggaagctagc catcgctggg ttagagtctc ggtgagacga
     1021 agctgacact ctgcattatg tttggtttga tctttggcta tctaactttt ataatagcga
     1081 caatggaaaa tttatattat ttaagtcgaa aaatatgcaa aatgactttc tttcaaataa
     1141 atatcagagg ataatagaat ctatagccga agccatactt gagattgaag atggtgcctt
     1201 ttccgttagc aaagctaaga ttcccgaatt cataaataat tatctttcat ccatgtctcc
     1261 tgatttaaaa cagagggcaa agatcttact gtctcttttc agatactccc cgtttttata
     1321 catgaagttg aaaacattcc cctcgctagg cttagaaaaa agacaaaaaa ttctacgtga
     1381 ttttatgaaa agtaacctcc gccctaagct gcttgtattt caaacactaa aaacactaac
     1441 cgtcatgggt tattacagaa atgaagaaac ctggaaagat attggctatg atggtcctac
     1501 aataaaacaa ttccctccta ctgagccaat tatattagaa aggggggaaa aattgaaaac
     1561 agcagaagat atttcgacag atattagaga aaaagccgat gtatgcgtgg ttggttccgg
     1621 tgcaggcgga gcggtgacgg caaaagaact aagccaaaag ggacttaacg taatccttct
     1681 tgagatggga ggatataata caagcaggga tttcaaccaa agagaagagg atatgtatcc
     1741 gctgctttta gcagagcagg gagcaagttc aaccggtgac tactcaatta acgtgattca
     1801 tggcaaaggg gtaggtggct caaccgttca taacactggg ctttgttaca gaacacctaa
     1861 ggagattcta gaattatggg aaagggaatg gaaggttgaa gggcttacct tcaacgaact
     1921 tgaccctttt tataatgacg tagaaaaaac ccttagtatc cagccaatta gaattgagga
     1981 agtaaatcct aataatgcta tagcattaga aggggcaaaa aagctgggtt taagtaccat
     2041 tcttcccaat cacaaccgca gtggcgagtg ccccggctgc ggattctgca tcctcggatg
     2101 tagctatgat aaaaagcaga gcatgcttgt aacctatatc cctctagcat ataaatacgg
     2161 aacaaagatt tatgccgatt gcatggcaga aaagattgag ttaaagaacg gaagggcaaa
     2221 aggagtttat gggagattct atagaaacgg aataccaact caccctattt acatcgaagc
     2281 cgacacggta gttgtaagtg gaggcgctgt caacacacct gggcttctgc tacgaagcgg
     2341 gattgattca ggaggcaaag tagggcaaaa ccttcacctt catccattta tctttgttgg
     2401 ggggatattc gatgagaaga tagagggatg gaaggggatc ccacagagcg ttgtagtaga
     2461 tgaattctta aagcaaaacg ggaaggggtt aaaagggtat cttataatgc ctggctttgc
     2521 tcatccaata ctatttgctt cactaacacc atcctttgga aaagagcata gacgtatcat
     2581 gaaactttac ccgcaatttg ctacccttgg agtccttctt catgattcat ccagtgggaa
     2641 gatttatgta aataaaaatg gcagattaag aatctcatac aaacttaaca aggatgacaa
     2701 aagagagcta acgcatggaa tgaagaagtg tgcttagatt ctatttgagg ctggtgctaa
     2761 agaggtaatc cttccatacg ttgagggtgt gttcataaaa tcgaagaaag atattgcaac
     2821 gattaaccaa ctaaaaatta taaagaatag aactcatatc atttcagttc atccgcaaag
     2881 ctcttgcccg atgggagaag acaagaaaaa atctgttgtg gactcatttt gcagatttca
     2941 cggaatagaa aatctatata tctctgatgc cagtgtgttc cctacatcaa ttggaatccc
     3001 tccacagatt acaatcatgg cgcttgctgc gagaacggcc aagtatattg caaaacaaat
     3061 gggaaggtaa ctactcaagg tgccactcta gttcgtgagg gatcactagg ctctttagcg
     3121 gatgtatatc cgctcatcct tgcctgttga agggcgggac tagaaagtct tggtcaagct
     3181 ggtggatgta ctatcagcac cactgtccca attggaacgg ccgtgactaa tcttactact
     3241 cccttaatac ctgcttttac gatcggtctt agagcgctaa aaagaaggag aaaggaagag
     3301 aagcaaatag aacacaggat gtaggggctg gtttaaaacc tgccctccaa aaagaattaa
     3361 agcgttatta aaaagctttc gtattttctt gctttttccc actcagaaac tcaagcatct
     3421 tgcttatgtc cgttgttgga agtttgcaga catagttaag gcagacgtat gccgtcgctt
     3481 taccctccaa gctcaattga gatttggtga actcagcaat gcgaattata tcaggctcct
     3541 ctttttctgt ttgacgaaga agaactacct tattgggcac aaattgtctt cttatagctt
     3601 ttatcatggc tttcgtatca tcagcctgtg agtcgcccac tatcactacc tcgtaagaag
     3661 gaccgacccc aaaatcaagg gctaccataa gctcagtatg agctgctgga aattgtttta
     3721 tggtttttga aaacgcactt ccaatctttt cagccatttc ttcaaaattg gaatttgttg
     3781 taatccggtc aagtctgagg aggttcaaca tagccgctga atttcctgat ggaatggcac
     3841 cgtcgtaaat ctccttatta cgaagaagta agttctcgcc atcgtccgct gtaaaataaa
     3901 aaccaccgtt tatatcatcc caaaagtgtg ttatcaaatc ccgatttaga tcaatcgatg
     3961 tttgaagata attaacatca aatgtggctt catatagctc aagcaacccc caggtaaaaa
     4021 aggcgtaatc atccacatga gctggtatgg cagcttgccc atctctgtag cgatgcagaa
     4081 gcctcccatc tggtttacgt aaattcttta ggatgaaatc aacggcacgt tttgcggcct
     4141 ccccatattt gggttcgttg agaacctgag caccttttgc cagtgcggca atcatgagac
     4201 cattccagtc ggttagaatt ttatcgtctt tatgtggatg aatgcgtttt tcacgaactg
     4261 aaaagagctt ctcccgtgcc gcctcgagac gctgttcgag ctcttgctcg gagatcttta
     4321 gctgagatgc aatcgcaggg attgattttt ttagatgtaa aatgttccta gcagtgcttc
     4381 ttccggttgc ctcctcgctg aaattgcccc cctcttctac gttaaacaca tcaatagtta
     4441 aatcggcttc ttctttgccc aaaaccgcac ggatttcctc ttccgtccat aggtaaaatt
     4501 ttccttcctc accctcgcta tcggcatcct cagcagagta aaacccacct tgaggtgagg
     4561 tcatatcacg caatacataa gtaaaaatct cacgtgccgc ttttttatat tcttcttttc
     4621 cggttacttg ataagcctct atgtaagcca tagcaagaag agcctggtca tagagcatct
     4681 tttcaaaatg gggcaaaaac cattctgcgt cagtagaata acgatgaaac ccaaatccga
     4741 catggtcgta aataccgccg aggcgcatcg cttgaagagt attctctacc atctccaagg
     4801 catcttcgtt actggcacgc ttccagtacc taagtaaaaa aagcaggttg tgaggagtag
     4861 gaaacttagg agccctacca aatccaccat gcatagtatc aaatcgctca gcaagctgat
     4921 tatatgcaac tctaagagta aattcgtcca gtttatcacc aagggattcc tttgtagcct
     4981 gctggagcgc tgaggttatt tgattagccg agtttacaat ttcatcacgt tttgttgtcc
     5041 aaatctcttt aatcctaggc actaattgcg tcatcccaat ccgtccaaaa cggctctctt
     5101 tagggaaata ggttcctgca aaaaatggct ttttatcagg tgtcataatg atagtgagcg
     5161 gccatccgcc gctgcctgtc atcatttggc agactgtcat atatatacta tcaatatcag
     5221 ggcgttcttc cctatcaacc ttgatagaaa caaatacctc gttcataagc tttgcaacct
     5281 cagggtcctc aaatgactca cgctccatta catgacacca gtggcaggtc gaatatccaa
     5341 tcgagagaaa tatcggttta ttttcccttt tagccttctc aaatgcctcc gctccccatg
     5401 gataccaatc tacggggtta tatgcatgtt gcagtaaata aggactcttc tcatttatta
     5461 gcctattagg tttctccatt gttgttgatt cagatgcttt caacatcgcc tcctggtttg
     5521 aagtgaagaa tacaaggaat cctataataa ctccagtagc aattgccgat aataacgcaa
     5581 ccatgcttct ttttagtttc atgctacaga ttccttgtaa tattgattaa catttgtaat
     5641 tctgcctcga catacttcct acctgaaatc ctcaacacca gtaaatcttc aatctaacgg
     5701 acttgtaatg acggcttcat tactaagtat acccttatgt ttggcaacaa tgatgaaaac
     5761 gctctccgga aatccctgga gacgaacagg atgagtacga acgggcatct gtgtctgcat
     5821 ctctacagac gattcaaggc taggaggttc agttggaaaa tttatgggtg attttaagtt
     5881 atttatgtat cttacatctc tcttttcttt tgtaactaag acctcaaaat gaagatattc
     5941 atccgtatta agcgtcaacc catttggaag agaaataagg ctgaaaacag caccatctct
     6001 tgccttgatt atgtcctgta tccttctttc tgaatc
//
LOCUS       DATDRL010000103         5364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1178098, whole genome shotgun sequence.
ACCESSION   DATDRL010000103 DATDRL010000000
VERSION     DATDRL010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178098"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VI935_03440"
     CDS             <1..201
                     /locus_tag="VI935_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013755782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLE24686.1"
                     /translation="EEGGYNVVIPAIPEICTFGETLEEAREMAKDAIHCFLESAIKAG
                     EPIPQDVKPKAEQVGVGLEVFP"
     gene            244..429
                     /locus_tag="VI935_03445"
     CDS             244..429
                     /locus_tag="VI935_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HLE24687.1"
                     /translation="MLLKGLVSIFIITGSHARLFHKTRPELRVTIPIHSKDIPKWTLK
                     RILKQADLTVEDFLKLL"
     gene            592..792
                     /locus_tag="VI935_03450"
     CDS             592..792
                     /locus_tag="VI935_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24688.1"
                     /translation="MSPYNIKINFPDGKTEMRKGKAGEAIWQEAETHPSENMGRADAR
                     ALIVELKEPNMKRAQKKKKKLS"
     gene            887..1150
                     /locus_tag="VI935_03455"
     CDS             887..1150
                     /locus_tag="VI935_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HLE24689.1"
                     /translation="MKTTIDAAGRLVIPKEIRQEAGLKPGMSIEVRWREGRIEIEPAP
                     LPVKLARKGRLLVAVPQEDVSPLTAETVERTRMRLRQERISHT"
     gene            1156..1605
                     /locus_tag="VI935_03460"
     CDS             1156..1605
                     /locus_tag="VI935_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HLE24690.1"
                     /translation="MPGFLPDTSCIVAAVCAWHEHHDRAAEEIERRLSRGEKMIVAAP
                     ALVEAYAVLTRLPPPHRLSTADTLALLEANFMRATRIVALEGKSYRTLLRGAPDEGII
                     GGRIYDVVIAACVFKAKAAVLLTFNESHFLPFVERGMEVVVPARDRL"
     gene            1753..3072
                     /gene="hemA"
                     /locus_tag="VI935_03465"
     CDS             1753..3072
                     /gene="hemA"
                     /locus_tag="VI935_03465"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:NF000744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HLE24691.1"
                     /translation="MRVILVGLNYKTTPVEVRERFSFSQSLLEAGLSKLSQVKNVEEC
                     LILSTCNRVEIYAASEKAEECVEDIKKFLSDFHKVALDHFSSYLYVMLDQTAVRHLFR
                     VASSLDSMVIGEPQILGQVKEAYRAAHIMRTSGLILNRLFHSAFFVAKRVRAETKIGS
                     QAVSVSYAAVELAKRIFDDLSKRTVMVIGTGDMAELAAKHLVAASIKDLVIANRTFES
                     AESLAYRLKGKAIRIEEVHYFLRNVDIVITATGSSDFIIRPQHVREALKLRKNEPMFM
                     IDIAVPRDIDPRVEEIPNIYLYDIDDLNGVLDENLKIRREHAKKAEEIVLQGEQDFQK
                     WINGLSVVPAIISIRKRFEEIKDLEVQKALRRLSSLSERERNVIEGMAAGIVGKILHN
                     PITNLKRETASSLGSLYVDTLNRLFELDGKDEFLEEEKDEASAKDWE"
     gene            3044..3967
                     /gene="hemC"
                     /locus_tag="VI935_03470"
     CDS             3044..3967
                     /gene="hemC"
                     /locus_tag="VI935_03470"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HLE24692.1"
                     /translation="MRQALRIGSRGSSLALLQAEFIRSLIKFKSPEIATELHIIRTTG
                     DKILDSPLSEIGGKGVFVKEIDDALIRNEIDIAVHSMKDLPTILPNGLTIGAVAERHD
                     PRDVLVSKNDLKFNELPKGAKVGTSSLRRQAQLLNLRPDLQIFPLRGNVDTRVRKVRS
                     EGLDSAVLALAGLERMGFKDEIAEIFPVHVLVPAPGQGALAVECREDHREINDIISQI
                     NHKESSISAYAERAFLAELGGGCQVPVGCYAQIKKDRINILGLIASTDGREIIREEID
                     GSVEIHQALGRELALRILNKGGRGILSKGVG"
     gene            4195..5022
                     /locus_tag="VI935_03475"
     CDS             4195..5022
                     /locus_tag="VI935_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HLE24693.1"
                     /translation="MLYENLSDITFAFLDVETTGLDPYMGDRVCEIAILKTRSGKILD
                     KFETLINPGRNMPPQAVSINGITNEMVKNALFFRDIAWNVINSLKGTVIVAHNASFDL
                     GFLLAEFSNIKLLPPENMVIDTLSIARRHYSFPSNSLGKIARHLGISVVGEHRAFGDV
                     RITKEVFEYFLLDLERRGIRIKRLRDMLKLQGGPIVFEKSNELVLPPVIEEALRAKGK
                     LQIKYLSAYSEATSTRIIEPLEVNLSGSFTYLLAFCHLRKEKCNFRLDRILEVKTIS"
     gene            complement(5042..>5364)
                     /locus_tag="VI935_03480"
     CDS             complement(5042..>5364)
                     /locus_tag="VI935_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24694.1"
                     /translation="QKGICKEVVNMRKVTAMFVALVMLLSVSIMAGCEGGRIKAEYEK
                     LKVEAEQLTKDKADLENKIQELTNQVNTLTTENEQLKSQLAALTQPSPAPEATPEAEE
                     MPAE"
BASE COUNT         1599 a         1008 c         1274 g         1483 t
ORIGIN      
        1 gaggaaggtg gttataacgt cgttatacca gccattccag aaatatgtac cttcggcgag
       61 accttggaag aggcaaggga gatggcaaag gatgctattc attgtttcct tgaaagtgcg
      121 attaaggcgg gagaacctat accccaagat gtcaagccaa aagccgagca ggtcggggtc
      181 ggtcttgaag tgtttcccta gctatgtcct ctattcctgt cttaaaaccc caagaagttg
      241 tacatgctct taaaagggct ggtttctata ttcatcatca ccgggagtca tgcaagactc
      301 tttcataaaa ctagacctga actaagagtt acaattccaa ttcactcaaa ggacatcccg
      361 aaatggacac taaaaagaat ccttaagcaa gccgacctaa cagttgaaga tttcttgaaa
      421 ctactttaaa ggaaatcggg gtcatgtata gagggaaaag tcaaaaggag ataaagtgat
      481 cactcgttgt ctagtttcag gcactatggt ataaccacct ggaaatcgtt tagaggcttt
      541 acatggagat cgagaaggtc aatatagcat ccggattctt ttatttactc attgagccct
      601 tacaacataa agatcaattt tcctgatggc aaaactgaaa tgcgtaaggg aaaggcgggc
      661 gaggcgatat ggcaggaggc ggaaacacac ccttcagaga acatgggaag agccgatgct
      721 cgtgctctga ttgtcgaact gaaagagcca aatatgaaga gggcacagaa gaagaaaaag
      781 aaactgtctt gattcttcga gcaactcgaa aacggttcta taatctccta ctaaggggtt
      841 caatacgtta ttgagactag gaaaattata tggtaaaatc tttaccatga aaactaccat
      901 cgatgctgcc ggacgactag tcatccccaa ggagatcagg caggaagctg gacttaagcc
      961 cgggatgtcg atagaggtac gctggcggga aggcaggatt gagatcgagc ctgcgccgct
     1021 gcctgttaag ctagcgcgca aaggtcggtt gcttgttgcc gtaccacaag aggatgtgag
     1081 tccactgacg gcagagacgg ttgagcgaac acgcatgagg ctacggcagg aacgcatttc
     1141 gcatacttaa cgattatgcc tggattttta ccggatacga gttgtatagt cgcagcagtc
     1201 tgcgcatggc atgaacacca tgatcgggcc gcagaagaga ttgagcggcg tttaagccga
     1261 ggcgaaaaga tgattgtggc ggccccagcc ttggtcgaag cctacgcggt gctaacccgt
     1321 ctacccccgc cacatcgact ttccacagcg gataccctcg ccctcctaga ggccaacttc
     1381 atgagggcta ctaggatagt tgccctagag ggcaaatcat accgtaccct cctacgtggg
     1441 gctccggatg agggcatcat tggtggacgg atctacgacg tcgtcattgc cgcttgtgtt
     1501 tttaaagcga aagctgcggt actactgacc tttaacgaga gccatttcct accctttgtg
     1561 gagagaggaa tggaggttgt tgtccctgca agggataggt tatgagggcc taagatataa
     1621 tggggttagg ctttgtattt gcgaatacaa ccatttttct ggataacgat ggcttatcaa
     1681 ggtctagttt atatgttata tatatttagc agtcgctaga gaaagttgaa agaaagcata
     1741 aatgattaaa ttatgcgtgt aattcttgta gggctaaact ataagactac cccggttgag
     1801 gttagggagc gattttcctt ctctcaatct ctgcttgaag cagggctttc aaaattatct
     1861 caagtaaaga atgttgagga gtgtcttata ctttctacgt gcaatcgtgt tgagatttac
     1921 gctgcatctg aaaaggctga agagtgcgta gaagatatta aaaaatttct ctccgatttt
     1981 cataaggttg ccttagatca cttctcttcc tatctttacg ttatgttaga tcaaacggca
     2041 gttaggcatc tttttagggt agcatctagt ttagactcta tggtgatcgg agagccacag
     2101 atcttgggcc aggtaaaaga agcctacaga gctgctcata ttatgcgtac atccgggctt
     2161 atccttaacc gcctatttca tagcgctttt tttgtggcaa aaagggttag agcagagact
     2221 aaaatcggaa gtcaggcggt atcagtaagc tacgctgctg ttgaacttgc aaaaaggatt
     2281 tttgacgatc tttcaaagag gactgtaatg gtcattggca cgggggatat ggcggagctc
     2341 gcggccaagc atcttgttgc ggctagtata aaggatttag taatcgcgaa tcgaacattt
     2401 gaaagtgctg agtcacttgc ttacaggctt aaggggaaag cgataagaat tgaggaggtt
     2461 cattatttct taagaaatgt ggatattgta attacagcca ctggttcttc agattttatc
     2521 attagaccac agcacgtgcg tgaggctctc aaactaagaa aaaacgagcc tatgtttatg
     2581 atcgacatcg ctgttccacg tgatattgac ccgagggtag aagagattcc aaatatctat
     2641 ctttacgaca tagatgatct aaatggagtg cttgatgaaa atctgaaaat aagacgtgag
     2701 cacgcaaaaa aggctgaaga gatcgttctt caaggagagc aggattttca aaagtggatt
     2761 aatggtttaa gcgttgttcc tgcaataata tcaataagga agaggtttga ggaaataaaa
     2821 gacctcgagg ttcaaaaggc gcttcgtaga ttatcaagtt tatctgaaag agagaggaat
     2881 gtaattgaag gtatggcggc aggtatcgtc gggaagattc ttcataaccc aataaccaac
     2941 ctaaagaggg agactgcttc ttctcttgga tctctttatg tagacacact caatagatta
     3001 ttcgaacttg atggcaagga tgaattttta gaagaagaga aagatgaggc aagcgctaag
     3061 gattgggagt agaggaagca gtcttgcgct cttgcaagca gagtttatta gaagtcttat
     3121 caagttcaaa tcccccgaga tagcaaccga gctacatata atccgaacaa ctggtgataa
     3181 aatcctggac tcgccgcttt ctgagatcgg tggtaaaggt gtgtttgtca aagagattga
     3241 cgatgccctc attcgaaatg agattgacat cgctgtgcat agtatgaaag atcttccaac
     3301 aatacttccc aatggactta cgattggagc agtggctgaa aggcatgacc caagagatgt
     3361 gctagtatca aaaaacgatt taaaatttaa tgagcttcca aaaggggcta aagtcgggac
     3421 gagcagtctc agacggcagg ctcaacttct taacctcaga cctgaccttc aaatttttcc
     3481 actaagggga aacgttgata caagagttag aaaggtaaga agcgagggac tagattctgc
     3541 ggttcttgca ttagcaggtt tggaaaggat gggatttaaa gatgagatcg ctgaaatatt
     3601 tcctgttcat gtgcttgttc ctgctccggg acagggtgca ttggcagtag agtgcagaga
     3661 agatcataga gaaataaatg atataatctc ccaaataaac cataaagagt ctagtattag
     3721 cgcttacgcc gaaagagctt tccttgccga actcgggggt ggttgccagg ttcctgtagg
     3781 ttgctatgca cagattaaga aagacagaat taacattctc ggcttgattg cttctactga
     3841 tggcagagag ataattaggg aggagattga cggttcggta gagattcatc aggcccttgg
     3901 aagagaactt gctctaagaa tacttaataa gggtggaagg gggattcttt caaagggagt
     3961 cggataaact gttaagcacc tctttctagt ttcggattta aatcacttct ctttgataat
     4021 tcgttttccg taacctgata tcagagtatc tgaataacaa aaccttgtag aaaccgaagg
     4081 gaatatccat atatccgata ccctgtagcc tgctgaatgg tattttattt ttaattttat
     4141 ttgtactcta ttttattttc gtgatattat gataaaaaca gacggagaaa aaatatgctc
     4201 tacgaaaatc tgtcagacat cacctttgct ttcttagacg ttgaaactac aggtcttgac
     4261 ccttatatgg gagacagagt atgtgaaatt gcaattctca aaacaagaag cggtaagatc
     4321 ttagataaat ttgaaacact tataaaccct ggaaggaaca tgcctcctca agctgtgtct
     4381 ataaatggaa taacgaatga gatggtaaaa aatgccctct tttttagaga catagcttgg
     4441 aatgttataa attcgctaaa aggaacagta atagtcgcgc ataatgcatc gtttgatttg
     4501 ggttttttgc ttgcagagtt ttcaaatatc aaacttttac caccggagaa tatggtgatt
     4561 gatacactca gcattgcaag gaggcattat agtttcccta gtaatagtct tgggaaaata
     4621 gcaaggcatc tggggatttc agttgttggc gagcacaggg cttttggtga tgtaaggata
     4681 acaaaggagg tttttgaata ttttcttctg gatttggaaa gaaggggtat aagaattaaa
     4741 cgattaaggg atatgcttaa actccaggga ggacctatag tattcgagaa atctaacgag
     4801 ctggttcttc caccagttat cgaggaggct cttagagcta aagggaaact tcagataaaa
     4861 tacctctcag catatagcga agccacctca acaagaatca tcgagcctct agaggtcaac
     4921 ttaagtggta gcttcacata tctcctagcc ttctgccacc taagaaaaga aaagtgcaat
     4981 tttaggcttg accgtatact tgaagtgaaa actatttcct gattttaatc agagaaaagt
     5041 tttattcagc aggcatttct tctgcctctg gtgtggcttc aggagcaggg cttggttgtg
     5101 ttaaagcagc aagctggctc tttagctgtt cgttttctgt ggtcaatgtg ttaacttgat
     5161 tagtgagttc ttgtatctta ttctctagat ccgctttatc ttttgtaagt tgttcagctt
     5221 ctactttaag tttttcgtat tctgccttga ttcgaccacc ttcacaacca gccattattg
     5281 aaaccgataa taacatcact aatgcaacaa acattgctgt aacctttctc atatttacta
     5341 cctccttgca aattcctttt tggg
//
LOCUS       DATDRL010000104         3088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_337357, whole genome shotgun sequence.
ACCESSION   DATDRL010000104 DATDRL010000000
VERSION     DATDRL010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337357"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="VI935_03485"
     CDS             <1..1825
                     /locus_tag="VI935_03485"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLE24695.1"
                     /translation="WSFKWAIDNILPNKRTTHPPLGLATLAALCPENWEVEIIDENIE
                     SIPLDPRADIIGVCGMGVQFKRQMELLTFYKRKGYYVVAGGSYASLCPELYEFLADTV
                     VAGEAEYIWKEFCKDFEREKPKRLYHEHGEVALTDSPLPRFDLLKLDKYQAVSLQFSR
                     GCPFRCEFCDIIVMFGRKPRTKSLEQVGRELDELRRLNINNVFFVDDNLIGNKPLAKK
                     LLKFLSEYQREHNHRFHFGTEASLNLAHDEELLELFRGANFGWVFIGIESPDEESLKE
                     TLKFQNTRQDMFASIKKVYSYGIEILAGFIVGFDNDTIETFDKQYRFITKSGIQTAMI
                     GLLTAAPKTPLYERLEKDGRLIPDANNSDNTKLSTNIIPKQMSYDEMVSGYRALYYRL
                     LDNRNIAARIKNKTRYISNPIYRGSYPLGEQLKVLERFFIHGILPGGFSRIFHFFRTI
                     PFSKPGQIPLVIQDWIVGLSMRDYIERHFIQEFEDVNHLSDSYLGLIEKAFQRYLNLG
                     ALEVSFNQVKNAAANLSISIKGLLDSDFYAGAAPHIEKVLKHTTSSVTLHIEEFHDAH
                     LEHLNRFLGRLSRYGDRIHIKVHERVRNMVDIDSSVFNLVL"
     gene            2243..2812
                     /locus_tag="VI935_03490"
     CDS             2243..2812
                     /locus_tag="VI935_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24696.1"
                     /translation="MKIIIAVITWLLLALVVGKYRLLATFAPPFPQVVLFGLTLILLL
                     LCWKLSVFRNWVFNLDIRVLVLVHLSRFIGIYFLILHSQGELPYAFAVPGGLGDIAVA
                     TTAIFVSLISSASGVKGFRILLIWNVFGLLDILFVIAAAGRLGMADPGSINALTRLPL
                     SLLPTFLVPIIIFTHIIIFVRLVKIRSQF"
     gene            complement(2907..>3088)
                     /locus_tag="VI935_03495"
     CDS             complement(2907..>3088)
                     /locus_tag="VI935_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HLE24697.1"
                     /translation="LERAEKVLSGLGTEIENFYKYSVISDELIGLRSAIQTANLIVHA
                     AKANTKSRGCHFIVD"
BASE COUNT          891 a          575 c          640 g          982 t
ORIGIN      
        1 ctggagcttt aaatgggcaa ttgataacat attgccaaat aaaagaacta cacatccccc
       61 tttaggatta gctaccctag ctgccttgtg tccggagaac tgggaggttg agattattga
      121 tgaaaacata gagtctatac ctcttgatcc aagggcagat ataatagggg tttgtggaat
      181 gggggttcaa tttaagcgac aaatggaatt gctgactttc tataaaagga aagggtacta
      241 tgttgttgca ggtggaagtt acgcttcatt atgtcctgag ctgtatgaat tcctagcaga
      301 cactgttgtt gcaggcgaag cagaatatat atggaaagag ttttgcaaag atttcgaaag
      361 ggagaaaccc aaaagattat atcacgagca tggtgaagta gctctcactg attctccact
      421 tccccgtttt gacctattaa agttggataa atatcaagca gtgagtttac agttttcaag
      481 aggatgtcct ttccgttgtg aattttgcga cattattgtt atgtttgggc gtaaaccccg
      541 cactaagtcc cttgagcaag ttggccggga gttagacgag ctaagaaggc ttaacataaa
      601 taacgtcttt tttgtagatg acaacttgat tggaaataaa ccacttgcaa aaaaactact
      661 gaaatttctc agtgaatacc agagagagca taatcatcgc tttcattttg ggactgaagc
      721 ctctttaaac cttgctcatg atgaggaact tcttgagctt ttcaggggag cgaattttgg
      781 atgggtcttt atcgggatag aatcccccga tgaagaaagc ctaaaagaaa cactaaaatt
      841 ccaaaatacc cgtcaagata tgtttgcttc aattaaaaag gtttattcct atggtattga
      901 aattcttgcc ggatttattg tcggttttga taatgacaca attgagacct ttgataaaca
      961 ataccgtttc attacgaagt cgggaattca aacggctatg attggacttt taactgcagc
     1021 gcctaaaaca cccctttatg agcggctgga aaaggatgga aggctgattc ctgacgcaaa
     1081 taattcagac aatacgaaac tgagcacaaa tattatacca aaacaaatga gttacgatga
     1141 gatggttagc ggatatcgcg cgctttatta tcggttgcta gacaatcgta atatcgcagc
     1201 tcggatcaag aataaaacac gttatatctc aaatcctatt tatagaggtt catatccatt
     1261 aggagagcag ttgaaggttt tggagaggtt cttcatccat ggaatattgc cgggcggttt
     1321 ttcgcgtatt ttccattttt tccgaactat tccattttcc aagcccggac agattccact
     1381 tgttatacaa gattggatag ttgggttgtc aatgagagat tatattgaac gtcattttat
     1441 tcaggaattt gaggatgtaa accacttaag cgacagctat ctagggttga tcgagaaagc
     1501 gttccagcgt tatcttaacc ttggtgcgtt agaggtatca ttcaatcagg ttaaaaacgc
     1561 tgctgctaat ctttccatat ccatcaaagg attgttggat agtgatttct acgcaggagc
     1621 agccccacat atagaaaaag ttcttaaaca tacaacctca tcagtaaccc tgcacataga
     1681 agagtttcat gatgctcacc ttgaacattt gaatcggttc ttaggacggc tttcccggta
     1741 tggtgataga atacacatta aagtacatga aagggtaagg aatatggttg atattgattc
     1801 ttctgtattc aatctcgttt tgtaaggttg attattaatt tttctagaat aaacctaccg
     1861 ggcgacatgt agtaaaaacc tcagtagtta cactttgctt ttgagcagat gattgcatta
     1921 aaggacggac gtctgtataa gagactaaca atccagcttt cgctctcgat ccttaagtgc
     1981 atacaggaag tagactaaat aaaccgctct gagtaggata acacgatggt aactcaggaa
     2041 agattataat gagttttttc taacaaaagt tcgtaggctc aagcccagcc cctaacaaca
     2101 agtctcattt tatggtaaga cagcttaacc cgtgttgcag tcggccccct cagagtttgg
     2161 gtgagcttgc cggttaaata attctcaagc gattaatttt tttgtcatta acaacatgac
     2221 aggcccaaag tcattgtact cagtgaaaat aataatcgct gttattacgt ggctcttgct
     2281 agctttagtt gtaggaaagt acaggcttct tgctactttt gcaccaccat tcccacaggt
     2341 tgttcttttt ggattaactt taattctatt attactctgt tggaagttga gtgtatttcg
     2401 taattgggtt tttaacttag acatcagggt tcttgtgcta gttcatttat ctcgctttat
     2461 tgggatttac ttccttatcc ttcattcgca aggcgaactt ccctatgcct ttgctgtgcc
     2521 cggaggctta ggcgatatag cagtcgcaac tacagcgatt tttgtatctt taatttcttc
     2581 cgcaagtggt gtaaagggtt ttagaatctt gttgatttgg aatgtattcg gcttactgga
     2641 tattcttttt gttattgctg ctgcaggaag attgggtatg gcggatccag gttcaattaa
     2701 tgcgttaacg aggctaccat taagcttgct ccctacgttt ttagtgccaa taattatttt
     2761 cactcacatt attattttcg tacgtcttgt aaagatccgg tcacaattct gagagtcttg
     2821 catttatcaa gaaaaatctc atgacctcac atttagtcct gaagaagcct ttaggtttag
     2881 attagctagg gattatctat ctcttatcaa tcaactataa agtgacaacc cctgctcttg
     2941 gtatttgctt ttgcagcatg gacaataaga ttcgctgttt gaatagcact tctaagccca
     3001 ataagctcat ctgagattac tgaatatttg taaaagtttt cgatctcagt gcctaatcct
     3061 gacagtactt tctctgcccg ttctaagc
//
LOCUS       DATDRL010000105         4161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_20630, whole genome shotgun sequence.
ACCESSION   DATDRL010000105 DATDRL010000000
VERSION     DATDRL010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20630"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(437..802)
                     /locus_tag="VI935_03500"
     CDS             complement(437..802)
                     /locus_tag="VI935_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24698.1"
                     /translation="MQEKIQGHGLKESIAMAFNLGVWNKQQKGHTGNVSEAAKELRDT
                     IYWNMFKQYGDAYPSDLLNANVEFFLEIALLGYILPGVCLPDEELKSRLLAHIEARAK
                     GEAPQQLIEQYSTATTFHN"
     gene            complement(1121..1540)
                     /locus_tag="VI935_03505"
     CDS             complement(1121..1540)
                     /locus_tag="VI935_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24699.1"
                     /translation="MAATKETQKVKDFTETVKGMTGLVKENYINGVEFALSLWEENLK
                     VFNAQVDQWLNLQNDYTNAVKELYERFPREAATLWDENPKAINGQVDRLVAFQKDYVD
                     SVRKVSDKFTKEALNLGQKNVEKAFSLIDDYIGLFRG"
     gene            2136..2975
                     /gene="lsrF"
                     /locus_tag="VI935_03510"
     CDS             2136..2975
                     /gene="lsrF"
                     /locus_tag="VI935_03510"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HLE24700.1"
                     /translation="MRKRGVIHMDWGMKNRMARIIRPETGRAVMLAVDHGYFLGPTTR
                     LEVPRRTIEPLIPYADTLMLTRGVLRTSVDPNSGTNVVLRVSGGVSIIGPSLADEGIT
                     TSMKDAIRLNVAGIGLSVFVGTEHERKTLLSLAELVNKGEEYGIPVLAITAVGRELEK
                     RDARFLSLCCRICAELGAHIVKTYYCEDFEKVSESCPVPIVIAGGPKLATELDALEMS
                     YNAVQQGAVGVDMGRNIWQSDHPVAMIRAIRAVVHENYTPKQAHDLFRSLKDAEGKKV
                     VSV"
     gene            3187..>4161
                     /locus_tag="VI935_03515"
     CDS             3187..>4161
                     /locus_tag="VI935_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HLE24701.1"
                     /translation="MYYNNRDVRLEEMPTPRIGSDEILVSVQASGVCGSDVMEWYRVP
                     KAPLVLGHEIAGAVVEVGEGVKNFKKGDRVFATHHVPCNTCYHCLRGNHSSCDALRST
                     HFDPGGFAEYIRVPAINVDRGVFLLPDEVSFEEGSFIEPLGCVIRGQRLARFQAGSSV
                     LIIGSGITGLLHIQLACAQGAGRIIATDINDYRLKAALRLGANAVIHANEDVPKSLRE
                     VNDGRLADLVIVCTGSQRAIEQSFNLVERGGSILFFAPTDPGTKIPIPFNEIWWAGIT
                     MTSSYAASPSDLAIAIELIRSRRVNVKDMITHRLPLAETGRGFQLVAEA"
BASE COUNT         1135 a          825 c          893 g         1308 t
ORIGIN      
        1 caaatctaat cctggacgat tgtaaatatc tccgaatggg aattcgagta tatagcgagc
       61 taggtcaggg tcaagctctt gtagtcgctc aatgatctgt tctcctttgt cgcctttaat
      121 ttctttaaac ttttctattc caatttcata tctatcttta ttcatatagt tctccttatt
      181 attctggtgt ttttaataaa attttataag ttgttttcgc ccatttcgtt ttttgcttct
      241 ttctgaaagc gttaaacaaa taaattatat cctctaattt agtttaaaat taatttttca
      301 ttctgccact taaaataagc ttagagatta aagtgcgata aacacacctc tttttgcctc
      361 ataaattgtt tgaagaagcc ttagtgtacg ttgatataat ttatagactg aacatagttg
      421 tgagtgattt aaagaactaa ttatgaaatg tagttgccgt agaatactgt tcaattagct
      481 gttgaggagc ctctccctta gccctggctt ctatatgagc aagtagtctg ctttttaact
      541 cttcatcggg aagacagacc cctggaagta tatatcctaa aagggcaatc tctaggaaaa
      601 attccacgtt tgcgtttagg agatcacttg gatatgcatc accatactgc ttaaacatat
      661 tccagtaaat cgtatccctg agttctttag ctgcttctga aacattacca gtgtgccctt
      721 tttgttgttt attccacaca cccaggttaa atgccattgc aatcgattct ttgaggccgt
      781 gcccctgaat tttctcttgc ataaaatatc ttacctcctg tgacctatag ctcaatttca
      841 attagacttt atgtcacaca tcccctgtta actcaacaat tttctgtacc ttactgcata
      901 gtttccagtg tcattccgag cacagcgagg aatcttaaag gggttccttc gttgcggagt
      961 ttatcctgag ctaggcgaag ggctccttgc aatgacgggt ccttgaagtg tcatggcaaa
     1021 cgaagtgaag caatctcaat cggattgctt cgttcgtttt actccctcgc gatgacgccc
     1081 ttttctgtca atgactaaat ggatcattgc cccagtcaag ttaacctctg aacaagccta
     1141 tataatcgtc gataagggaa aaagcctttt ctacattctt ctgcccgaga ttaagagctt
     1201 ctttcgtaaa cttatctgaa acttttctaa ccgagtcaac atagtctttc tgaaaagcta
     1261 caagacgatc aacttgaccg tttatagcct ttgggttctc atcccaaagg gttgcagctt
     1321 cccttggaaa cctttcatag agttctttaa cagcattggt gtaatcattc tgaagattaa
     1381 gccactgatc aacctgagcg ttgaaaacct ttagattttc ttcccaaagt gatagagcga
     1441 actctacacc atttatataa ttttctttca ctagtcctgt cattcctttt acagtctcag
     1501 tgaagtcctt tactttttgt gtttcttttg ttgcggccat ttgcctgccc tcctatttgt
     1561 ttaacttact tataagataa tacaggtggg ggcgcttgtc aagggtataa tacgcatttg
     1621 cgtataataa acccttataa caaattgtta ctattgataa tattatataa caaatatatt
     1681 tatacgcaac tgctaatagt atgatggcaa aattatacac catatttggt taatattttc
     1741 ttcacaacat ctacgggatt aagcttaaat ttattgccgt gttggtgagg aataataatt
     1801 ctccataacg ataaatctta ggagcacctt ctagatttga tattattcaa cctggatagt
     1861 aagaatagct ccttctgggt aggcaaccag ccttgctcgt tgccattaag cttgtggaca
     1921 aaccgatgtt catatggtta agtggcaatg cacattaaag aaaagattaa actggaatta
     1981 tttgcacttg aagtaactaa tgatttgata ttaaatttct agggtaaatc catagtgttt
     2041 gccccctact cgtcgggcat actcatagga actatttttt gtgcgacctt gagtttgact
     2101 ttttttctac attactttaa agtttgtctg aaaaaataag aaagcgaggt gtcattcaca
     2161 tggactgggg aatgaaaaat cgtatggcta gaatcataag acctgaaacg ggacgcgccg
     2221 ttatgctagc agtggatcac gggtattttt tagggcctac aacgagattg gaggttcctc
     2281 gtagaacaat tgaaccatta attccctatg ctgatacttt aatgcttact cgcggagtcc
     2341 tacgaacctc agttgatccg aatagtggaa caaatgttgt gctaagggtc tctggtggcg
     2401 tgagtataat tggaccgtct cttgccgatg agggaataac cacctctatg aaggatgcca
     2461 tacgtctaaa tgtcgcaggt attgggttat cagtatttgt tggaaccgaa cacgagcgta
     2521 agactctact tagtcttgca gagcttgtaa ataaggggga ggaatatggt atccctgttt
     2581 tagcaataac tgccgttggc agggaacttg aaaaacggga tgctcgtttc ttatccctct
     2641 gttgtcgaat atgtgctgaa ctcggtgcgc atatagttaa gacatattat tgtgaggatt
     2701 ttgagaaggt gagtgaaagc tgtccagtcc ctatagtcat tgcaggtggg ccaaagctcg
     2761 ctacagaact agatgctctg gagatgtcct ataatgcagt tcagcaggga gctgttggag
     2821 tggatatggg tagaaacata tggcagtcgg atcatcctgt ggcaatgatc agggctatac
     2881 gtgctgttgt ccatgagaat tacacaccaa aacaggcgca tgatctattt aggtcgctta
     2941 aggacgcaga gggtaaaaag gtagtaagtg tctagagatt attgttccga tttaaaactt
     3001 taataccaaa tatgtattgg tcacgagcaa gactcgtgat ctaccctaaa cgttgataga
     3061 aaataaaaga cggggtagtc ccaccagtct tgctggtgga gttatattat gaatgctata
     3121 aatccaatgt ctcaatagca acattgaata aaatctaagt ccttaaatac taacatgcga
     3181 gtagctatgt attataacaa ccgggatgtg cggcttgagg agatgccaac tccccgtatc
     3241 gggtctgatg aaatccttgt aagtgttcag gcaagtgggg tttgcggaag cgatgtgatg
     3301 gagtggtatc gtgttcctaa agcaccgcta gtgctagggc atgaaattgc gggggctgtc
     3361 gttgaagtag gcgagggtgt taaaaatttt aaaaaaggag accgtgtttt tgccactcat
     3421 catgttccgt gtaatacctg ctatcactgc cttagaggta atcactcttc gtgcgatgcg
     3481 cttcggagca cacatttcga cccgggagga tttgccgaat atatccgtgt gcctgctatt
     3541 aacgttgatc gcggggtgtt tctacttcct gacgaggtct catttgaaga agggtcattt
     3601 attgaacccc ttgggtgcgt catacgcggt cagagattag cacgattcca ggctggtagc
     3661 agcgtgctaa ttatcggtag tgggattacg ggcttacttc atatccagct tgcttgtgct
     3721 caaggcgcag ggcgtataat tgctacggat ataaacgatt ataggttaaa ggcagctctc
     3781 cgtttggggg ccaatgctgt tatacatgct aatgaagatg tgccgaaaag tctgcgtgag
     3841 gtaaacgatg ggcgtcttgc cgatcttgta attgtttgta ctgggtcgca acgggctata
     3901 gagcaatcat ttaatttggt tgagcgaggc ggcagcattc ttttctttgc cccgacagat
     3961 ccgggtacaa agatcccaat tccgtttaat gaaatctggt gggctggaat caccatgaca
     4021 agttcctatg ccgccagtcc ttcagacctt gctatagcta tagagcttat tcgatcacga
     4081 cgtgtaaatg taaaagatat gatcacacat cgccttcccc tcgctgaaac aggccgaggc
     4141 ttccaactag ttgccgaagc a
//
LOCUS       DATDRL010000106         9369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3247684, whole genome shotgun sequence.
ACCESSION   DATDRL010000106 DATDRL010000000
VERSION     DATDRL010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9369
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3247684"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1497)
                     /gene="lysS"
                     /locus_tag="VI935_03520"
     CDS             complement(37..1497)
                     /gene="lysS"
                     /locus_tag="VI935_03520"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HLE24702.1"
                     /translation="MENELVEQRKQKIKELISIGVNPFPNDFKPSHDAGNVLSSYSNF
                     SDDEIKNLSDEFSLAGRVIALRDFGKSTFFHVMDRTGKIQGYIRRDVIGDEKLDFFKK
                     FIDIGDFIGIRGDLFKTKTGDLTINVKDFKLITKSLYPLPEKWHGLKDVETRYRHRYL
                     DLISNPEVKEIFLKRTKIIKLIRDFLNERGFIEVETPILHPIAGGAAAKPFKTYHNAL
                     DMELFLRIAPELYLKRLIVGGFERVYELGRTFRNEGVSTQHNPEFTIMELYQSYATYE
                     DLMDLIEELICFVTETLLGGIEFDYQDMKVNMKRPWSRINLIESVKSRLGDEILSSNE
                     FLFKKATSLGIAHKGIRGKAIIELFEHTIGYEIVDPTFVYGFPVDVSPLARKNDLNPE
                     ITDRFELYIAGREVANAFSELNDPIDQKERFLKQLDFKSKGEEIHEMDEDFIGALKHG
                     MPPTAGAGIGVDRLVMLLTNSPSIREVIFFPHLRPE"
     gene            complement(1684..2103)
                     /locus_tag="VI935_03525"
     CDS             complement(1684..2103)
                     /locus_tag="VI935_03525"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HLE24703.1"
                     /translation="MNDLHRKKEIKLPFPTPPFWELLGIEVVEIDDGYAKLVMPFHGK
                     LTQPYGMVHGGAIFSLADSAVAIATISMVEPERKVLTIEMKINFLAPVKDGVMEAKAR
                     VIRKGRIIPAEVDITNNGVLVAKALATYIILGDEKKL"
     gene            complement(2707..4215)
                     /locus_tag="VI935_03530"
     CDS             complement(2707..4215)
                     /locus_tag="VI935_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLE24704.1"
                     /translation="MNRRDFLKAVLGTSGLIAVWPAWGSRSITKPEWVVVKKPGGVSV
                     NDIHTELNPTIVDRIVYPNSLDAIQETIRGARRDGKVICVAGARHAMGAQQFATHGVL
                     IDMTAMSRVLNFNPDESTIEVEAGIQWPQVIGYLLKAQEGHPKRWGIAQKQSADWFSM
                     GGSLGSNVHGRGLQMKPFIQDVESFVLIDANGKEKTCSREENPELFRLAIGGYGLFGI
                     IYSIKLRLVPRQKVERVAEVMRVEGLMSAYEKRIADGFLYGTFLYSANPKSEDFLRKG
                     ILVCYRPVNSDKPVPDYPEQPSNEAWINLRRLAHVDKEQYFKKLSSSYLSTSGKIYWS
                     DTHQMGINLKGYHRKLDKMLCAPRSSEITTEVYVPGQSLTDFVEEVHEDFRKNRVDLV
                     FGNMGVIGQDNESFLAYLRNPFASVDFNIHTLHTPEGIERSRQALRRLIDMTVRRGGS
                     YYLTHHKFATPEQIKACYPQFPEFLRLKKQYDPEERFQSDWYRHYKEMFAKL"
     gene            complement(4340..4804)
                     /locus_tag="VI935_03535"
     CDS             complement(4340..4804)
                     /locus_tag="VI935_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24705.1"
                     /translation="MKNLTHSIMGTLLLVTLTLVFVTKTAGSGVSTIESGIKREGFQE
                     TAMAQDVVKVAPEHYKVLLENDKVRVLEVRIKPGEKEAMHTHPATVHIELVPTKVKIT
                     YPDGKSVILEGHLGEAIWVGPVKHTIENVGDTEIHAYIIELKEIPYEEKVSK"
     gene            complement(5274..6461)
                     /locus_tag="VI935_03540"
     CDS             complement(5274..6461)
                     /locus_tag="VI935_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLE24706.1"
                     /translation="MSRYSNDLIQFDMENKSLLNRNFILITLSSFIFFFNFHSFLLLP
                     IRIQELGGSASAIGFIMGATGLSTIFTTPAVGVLVDRRGKKRFLAFGGLIMSLTTLPF
                     AYLNTLNFLFPLLRIIQGAAFSLCFISAGTLIADVTPVLRRSQALGLFGVFTIVNYAL
                     APYFGKLIIETYSFKVFFLVFFIFGFISFLVALLIREPYRGMKTEIEGDSLLTTLFRK
                     GVFVSAFTLMIAGSGFIPTLTFFPVFAKDIKVESFHLFFIAYTLSALFVRLFGGWIPD
                     RFGKKRAVIPSLFFFSLSVIWIGFATSSSDFIETGVLFGLSHGLLYPSVYALVIDLSP
                     IVDRGKAFAICSVAFTLGGMLGSFIYGVVADFFGFQVMYIAAGGVCFLGFVIFSVFGR
                     EGG"
     gene            complement(6581..7846)
                     /locus_tag="VI935_03545"
     CDS             complement(6581..7846)
                     /locus_tag="VI935_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HLE24707.1"
                     /translation="MPENLKLLESLLIALALGVLIGAERGIARVRQRVEEKTTFAGIR
                     TFTLIALLGALSLHFTNYFGRMFFVVSFFGFVLLLTAAYIITSKKEKDAGTTTEITAI
                     LTFFYGALCMTEYRLVAIVLAIFTTVILYTKKPAYAFLGRITDEEFYATLKFAIIAFV
                     VLPFLPKDDYLGFFNPYRIWLIVVIISGIEFVGYVIMKLMPPRKGIAIIGFLGGIISS
                     TALVLNASRESKREKMLTNSLAFSAVLASSTVFLKLILEVYIVNKAVVDKAALPMIIM
                     FIFGMIGALFLWKRSKAEEEIKTIDLKSPFTLRPALKFAIFFAFIIFLVTFANKYLGV
                     KGVYVTSAIGGLANLDAPTVSLASLAYKQITAEVATLGILIAACINTVSKAGLAFLLG
                     SKGFSKLVILSLSFPIIGAILYIVVFMYG"
     gene            complement(7894..9177)
                     /locus_tag="VI935_03550"
     CDS             complement(7894..9177)
                     /locus_tag="VI935_03550"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HLE24708.1"
                     /translation="MKILVKGGQLVDPSQEINEIRTLYIEDGQIKSYPRGTKELEKDP
                     SIKVIDASGKIVSPGFIDLHVHLREPGFEHKETIRSGCTAAAAGGFTSVVCMPNTNPV
                     NDNASVTEYILLKARTEGIVNVFPVGAITKGQKGEELSEIGEMWEGGCVAISDDGRPV
                     MNSRVMRHAMEYAKVFGLPVISHAEDLNLSSGGVMNDGFTSTMLGLRGIPNASEEVMV
                     ARDITLAELTRTHLHIAHVSTAGAVRLIRDAKKRGIKVTAEATPHHFTLTDKAILSYD
                     TNAKVNPPIRSREDMEAIREGLKEGVIDIIATDHAPHTEDEKKVEFDLAPFGISGLET
                     ALPLALKLVEDGILTLTELIRKLTINPAQIIKRDRGTLKLGATADVVIFDPNKDVKVD
                     RDKFRSKGKNTPFHGWRLSGEVLYTIVGGQIVFPT"
     gene            complement(9174..>9369)
                     /locus_tag="VI935_03555"
     CDS             complement(9174..>9369)
                     /locus_tag="VI935_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HLE24709.1"
                     /translation="LTRERLRLAKEDVIVMHPGPINRGVELSSEVADDVHSVILNQVS
                     NGIAVRMAMLYLVASSGGNA"
BASE COUNT         2790 a         2121 c         1701 g         2757 t
ORIGIN      
        1 atcttaggta tctaagtcct ataaagaatt cgtatctcat tctggcctga gatgggggaa
       61 gaaaattact tcccttatgg aaggagaatt tgtaagaagc ataactaggc gatcaactcc
      121 tattcctgca cctgcagttg ggggcatccc atgtttaagc gctccgataa aatcctcgtc
      181 catttcgtga atctcttcac ctttcgattt aaaatcaagc tgttttagaa atctctcctt
      241 ttgatcaatt gggtcattaa gctccgaaaa tgcattcgct acttcccgtc ccgcgatgta
      301 aagttcaaat ctgtcagtaa tctcaggatt taaatcattt tttcttgcaa gaggtgacac
      361 atccacagga aatccataaa caaatgttgg atcaacaatc tcatacccaa ttgtgtgttc
      421 aaataactcg atgattgctt tccctcttat tcccttatga gcaatgccta aagaggtagc
      481 ttttttaaaa agaaattcat tactacttag aatttcatca ccaagcctac tcttaactga
      541 ctcaataaga ttaatcctac tccaaggccg tttcatattc accttcatat cttgataatc
      601 aaactcaata ccacctagaa gggtttctgt cacaaagcag atgagctctt cgattaaatc
      661 cattaagtcc tcgtatgttg cataggattg ataaagctcc ataatagtaa attcaggatt
      721 atgttgagtt gaaacccctt cgttcctaaa tgttctacca agctcataga ccctttcaaa
      781 acctccgact attaaccttt ttagataaag ttcaggagcg attctaagaa acagttccat
      841 atcgagtgca ttatgatagg tcttaaaggg ttttgctgca gcgccgcctg cgattggatg
      901 aaggataggg gtttcaacct cgataaaacc cctttcgttt agaaaatctc ttatgagctt
      961 tatgatctta gttcttttta gaaagatctc ttttacttca gggtttgata ttaaatctag
     1021 gtatctatgt ctatatcttg tctcaacatc tttaagtcca tgccatttct caggaagtgg
     1081 atataggctc tttgtaataa gtttaaaatc ttttacgttt attgtaaggt cacccgtttt
     1141 agttttaaat aggtctcctc taattccgat aaaatcaccg atgtcaatga atttcttaaa
     1201 aaaatcgagc ttttcatcac ctataacatc ccttcgtatg tatccttgga tctttcctgt
     1261 tctatccata acatggaaaa atgtgctttt gccgaagtcc cttagagcta taacacgacc
     1321 agcgagagaa aactcatccg ataggttttt tatttcatca tcagagaagt ttgaataaga
     1381 agatagaacg tttccggcat catgactagg tttaaaatcg ttcggaaaag gattaacacc
     1441 tatacttatt aattctttta ttttttgttt tctctgttct acgagttcat tttccataat
     1501 ttgagctaat ttttctacct aaaaagggaa aatacatcga gaaggacgag caccaattac
     1561 taaatctcaa attccaaaaa attctaattt tcaaatacca aagtttggaa ttgataattt
     1621 agggattgaa aattgtttga gacttgtgat ttagcatttg aaatttgtat tttcatcagc
     1681 agcttataat tttttttcat ccccaagaat tatatacgtt gcaagtgctt ttgcaacaag
     1741 taccccatta tttgtgatat ctacttcggc aggaataatt cttcctttcc taataaccct
     1801 tgccttagct tccatcaccc catcttttac aggagctaaa aaatttatct tcatttctat
     1861 tgttagtact ttcctctcag gctcgaccat agagattgtt gcaatagcta cagcagaatc
     1921 agcaagagaa aatatcgccc caccgtgaac catgccatat ggctgagtaa gcttcccatg
     1981 aaaaggcatc acgagcttgg catacccatc atctatttca acaacttcaa ttccaaggag
     2041 ttcccaaaac ggtggagttg gaaatgggag ttttatttct ttttttctat gtaggtcatt
     2101 catcttttct ccctatacca tatccaaact gcgccctaag ctcacaagct tattgttcat
     2161 cttttatctt ttttcagtcg aacgacaagg atcaatagac aaacaactaa aaactatcta
     2221 ataagcggaa ttgtcagtag tgagatcaat aactatttaa ctctaattca aactttttta
     2281 ttttaccaca ccctactcct ttccccatcg gcacacaatt ctaaatccaa gattcaaaga
     2341 atatttttac accctggaaa aattatttat ttcctaccca tttttgtcaa aaaaatagta
     2401 agaacaaaat aatgaataac acaattattg attgggtata aaccgatgat agatttaaat
     2461 ctaactaaat atgctgtatt accaatccca gtgtttattg aagtagatga agtcgagtcg
     2521 atttaataac acctcatatc tttaaaacct acttgtttaa ggcacaccat ctgctcacct
     2581 taatatgcac ttcctttggg aacttggtag tacacaactg gtactaaatt gagtgaaata
     2641 aaatagttat taaatcgttc ttctatctat cataataggc tatcatgcac ctgtaataat
     2701 ggcttttcat aacttcgcaa acatctcctt gtagtggcgg taccaatcac tctggaatcg
     2761 ctcttcagga tcgtactgct ttttcagccg gaggaattct ggaaattgcg gataacaagc
     2821 tttaatttgc tcgggtgtgg cgaacttatg gtgcgtgaga taataactcc ctccgcgccg
     2881 aactgtcatg tcaataagac ggcgcagtgc ctgacgagaa cgctcgatgc cttcaggggt
     2941 atgtaaggtg tgaatattaa agtcaacgct tgcgaaaggg ttccttaagt aggctaaaaa
     3001 gctttcatta tcctgtccga ttacccccat gtttccgaag accaaatcca cacggttttt
     3061 acggaaatct tcgtgcacct cttccacaaa atccgtgagc gattgcccag gcacgtaaac
     3121 ttccgtggta atttcgcttg accggggagc acacaacatt ttatccaatt tcctgtggta
     3181 gcctttaagg ttaatcccca tctggtgcgt gtccgaccaa tagattttgc cggaggtgga
     3241 aagataactg ctggatagtt ttttaaaata ctgttcttta tccacatgag cgaggcggcg
     3301 gagatttatc cacgcttcat tagaaggttg ctccgggtag tcaggcacgg gtttatcaga
     3361 gttcaccggc cggtagcaaa cgaggattcc cttccgaaga aaatcttcgg actttgggtt
     3421 ggcggagtaa aggaaggtgc catacagaaa accgtctgca attcgtttct cataagccga
     3481 catcaaaccc tctactctca taacctcggc aacgcgctcg actttctgcc ggggaacgag
     3541 tcgaagtttt atggagtaaa tgataccaaa caaaccgtag ccgcctatgg caagtcggaa
     3601 gagttcggga ttctcctctc ggctgcatgt cttctctttc ccatttgcat caatcaggac
     3661 aaaagactcg acatcctgaa tgaacggctt catctgcagt ccccggccat gcacgttgga
     3721 accaaggctc ccgcccattg aaaaccaatc agctgactgt ttctgagcga ttccccaccg
     3781 ctttggatga ccttcttgcg ccttgagtaa gtagccaata acctgcggcc actggattcc
     3841 ggcttcgacc tcgatagtac tctcatcagg attgaaatta agcacccggc tcattgccgt
     3901 catatcaatt agaactccgt gagtggcaaa ctgttgtgcg cccatagcat gccgtgcccc
     3961 tgctacgcaa attacctttc catcgcgacg tgcgccacgt atagtctcct gaatcgcatc
     4021 aagggaattt ggataaacga tgcgatccac aattgtcggg ttaagttcag tatgaatatc
     4081 gttgaccgat acccctccag gttttttgac tacgacccat tcgggttttg ttattgagcg
     4141 tgaaccccaa gctggccata ctgctataag cccggacgtc cctagaaccg ccttcaaaaa
     4201 atctcttctg ttcaactcag cctcactttc atgacattaa tgtcggaaga taatagcttc
     4261 tttgcatcac cacccttccc taagagttga aatacaaacg ctctctcccg taagtacttg
     4321 agaaaagcgg cctttgattt tatttgctca ccttttcttc ataggggatt tctttaagtt
     4381 caataatata ggcatgtatt tcagtatctc ctacgttctc tatagtatgc tttaccggac
     4441 cgacccatat tgcttcccct agatgtccct cgagtattac gcttttgcca tcgggatagg
     4501 tgattttgac cttagtgggg accaattcga tgtgcacagt agctggatga gtatgcatcg
     4561 cctctttctc tccgggttta attctgactt cgagtactct caccttgtca ttctcaagca
     4621 gcactttata atgctccggt gccactttta ccacatcttg agccattgcg gtttcctgaa
     4681 aaccctccct cttaatcccc gactcgatcg tggatacccc agatcctgct gttttcgtga
     4741 caaaaaccaa cgtcaaggtg actaacaaca aagtgcccat aatagaatgg gttaagtttt
     4801 tcatgattga tcctcctttt atttctaact tatcccgttt cagatacata agcaagtaga
     4861 gtacgttaat tatttgtgca aggattttaa gctggcctcc gcaggcatac ctggaaggta
     4921 caggcaatgc tcatgatcgt gcttggattc cgcgcgaagg aatccagccc tgaaacaatc
     4981 gcatctatct cggtgtgtga cgccagcccc gcccctacca acaatcccct aatatgctcc
     5041 atagtaactt tagttactag cttgccctca ccttgataaa acgtcggctg tgaaacatcg
     5101 acgcacactg gctcaactcc cgcctcttct aatattctgt acagtcgcgg tcaaatggct
     5161 gggtccccat catcctgtcg tacacccgct tggtatagtt gcaagtagcg cttgaaagca
     5221 gaacaggtcg gacaacaaac atgccttgga aggtctatgt tttccaccat aatttatcca
     5281 ccttctcttc caaatacgct aaaaattacg aagccaagaa agcaaactcc accagcggct
     5341 atgtacataa cttgaaaccc aaaaaaatct gcaaccaccc cataaataaa agatcctaac
     5401 attccaccaa gggtaaaagc tacactgcaa atagcgaatg ccttccccct atcaacaatt
     5461 ggagaaagat caataaccaa agcatagact gaaggataaa gaaggccatg acttagtccg
     5521 aataaaaccc cagtttctat gaaatctgac gaactggtgg caaagcctat ccatataaca
     5581 cttagagaaa aaaagaataa tgagggtata acagcccttt tttttccgaa cctatcagga
     5641 atccatcccc caaacagtct aacaaatagc gccgaaagcg tataagctat gaagaagaga
     5701 tgaaatgatt caactttgat atccttggca aaaacaggga aaaatgttaa ggttggaata
     5761 aaaccagaac ccgcgatcat aagagtaaat gcagaaacaa ataccccttt gcgaaagaga
     5821 gttgttagga gactgtctcc ttctatctcc gttttcattc ctctatatgg ttctcttatc
     5881 agtagggcga ctaaaaagga aataaaaccg aaaatgaaaa agacaagaaa gaaaacttta
     5941 aaactgtaag tttcaataat taatttccca aagtagggtg caagagcata atttactatc
     6001 gtaaatactc cgaaaagacc aagtgcctga cttcttctta atacaggtgt gacatcagct
     6061 ataagggtcc ctgctgatat aaaacagaga gaaaaagcag cgccttgaat aatccttaac
     6121 agaggaaata aaaaattaag tgtattaagg taagcaaatg gaagagtcgt aagagacata
     6181 atgagcccac caaaagcaag aaacctcttt tttccccgcc tgtctaccaa tacccctaca
     6241 gcaggagttg tgaaaatcgt actaagtccc gtagcaccca ttataaaccc tatcgcagat
     6301 gcgctgccac cgagttcctg tatacgtatg ggcaaaagga gaaaggaatg aaaattaaag
     6361 aaaaaaataa agcttgaaag tgtgattaag ataaaatttc ggtttaggag agatttgttt
     6421 tccatatcaa attgaatcaa gtcatttgaa tatctagaca ttaggttcct tcgtgatacg
     6481 tgtatcgtgg ttagtaattc gaataatcag tcccgaatcg cgagacataa accacgaatc
     6541 aggtattttc taattaggat aggctctaca aactgagcgt ttaaccatac ataaacacaa
     6601 caatgtataa tatcgctcct attattggaa aagaaaggga taatattaca agttttgaaa
     6661 agccttttga accaaggaga aacgccaatc ctgctttcga taccgtatta atacaggctg
     6721 caatcaatat gcctaaggtg gcaacctccg ctgttatttg tttataagcg aggcttgcaa
     6781 gtgagactgt tggtgcatcg aggtttgcaa gtccgccgat ggcagatgta acataaacac
     6841 cttttacgcc caaatacttg ttcgcaaatg ttacaagaaa aattataaac gcaaaaaaaa
     6901 ttgcaaattt tagtgcgggc ctaagtgtaa atggactttt taaatcaatt gtctttatct
     6961 cctcctctgc cttgctcctt ttccataaga agagtgcccc tatcattcca aaaataaaca
     7021 tgataatcat tggaagtgcc gctttatcca ccacagcttt gttaacaata taaacctcta
     7081 atattagctt taaaaaaact gtcgatgatg caagtacagc agaaaaagcc aaagaatttg
     7141 taagcatctt ttctctttta ctttctcgtg aggcatttaa aactaaagca gtgcttgata
     7201 tgattcctcc caaaaaccct atgatggcaa tgccctttct cggtggcata agtttcatga
     7261 ttacatagcc cacaaactca atacctgaaa taatcaccac gataagccat attctatagg
     7321 gattaaaaaa tcctaaataa tcatctttgg gtaaaaacgg aagaactaca aaagcgatta
     7381 tcgcaaactt aagggtagca tagaactcct catccgtaat acgacctagg aatgcgtaag
     7441 caggtttttt agtatagaga ataacagtgg taaagatcgc aagcacgatc gcaacaagac
     7501 gatattcagt catacataat gcaccgtaaa aaaaggttaa aatagcggtg atctctgtag
     7561 tagtccctgc atccttctct ttttttgatg ttatgatgta tgctgctgta agaagcaaaa
     7621 caaaaccaaa aaatgaaact acgaaaaaca tccttccaaa atagtttgta aaatgtaaag
     7681 aaagcgcacc taaaagagct ataagggtaa aagtccttat tccagcaaag gtggtcttct
     7741 cttccactcg ttgacgaacc ctagcaattc ctctttctgc accaataaga acaccaagag
     7801 caagagcgat gagaagcgac tccaaaagct ttaggttctc tggcatgagc taattatggc
     7861 aagaattcac tcttgattgg ttagttagcg gaatcaagtg ggaaatacta tttgccctcc
     7921 aactatagtg taaagaactt ctcctgaaag ccgccatccg tggaagggag tatttttgcc
     7981 ttttgagcga aatttgtctc tatctacctt aacgtcttta ttcggatcaa atataactac
     8041 atcggcagtg gctcctagtt ttagcgttcc tctatccctt ttaattattt gagctggatt
     8101 aatagtgagc ttccttatta actcagtaag agttaaaatg ccatcttcga caagcttaag
     8161 cgcaagagga agagcggttt caagcccaga gattccaaat ggcgctagat cgaactcaac
     8221 ctttttctca tcctcagtgt gtggggcatg gtctgttgct attatatcta ttacgccctc
     8281 ttttaaacct tctcttatcg cctccatgtc ttctctgctt ctaatagggg ggttcacctt
     8341 agcatttgta tcatagctga gaatggcttt atcagtgagg gtaaaatggt gtggcgttgc
     8401 ttctgctgtt actttgatcc ctcgcttttt agcatctctt ataagcctta ccgcgcctgc
     8461 tgttgaaaca tgggctatgt ggagatgggt tctagtaagc tctgcaagtg ttatgtcgcg
     8521 cgcaaccatt acttcctctg atgcgtttgg aattccccta agaccaagca tggttgatgt
     8581 gaagccatca ttcattacac cacccgatga aaggtttaaa tcttcagcat gagagattac
     8641 tggaagccca aacaccttag catattccat tgcatgtcgc ataaccctag agttcatcac
     8701 aggtcttccg tcatccgaga ttgctacgca accaccctcc cacatctctc caatctcgga
     8761 taactcctca cccttttgtc cctttgttat agcacctaca gggaatacat ttacaattcc
     8821 ctctgttcgt gccttaagta gtatgtattc cgtcaccgat gcattatcat taacaggatt
     8881 cgtatttggc atgcaaacaa ccgatgtaaa accacctgcc gctgctgcag tgcatccaga
     8941 gcgaattgtt tctttatgct caaaaccagg ctccctaagg tgaacgtgaa gatcaataaa
     9001 cccaggcgag acaatcttcc ccgaagcatc tatcaccttg atgctcggat ctttttctaa
     9061 ttccttagtg cctcgtggat agctcttgat ttgtccgtct tctatataaa gggtccttat
     9121 ttcattaatt tcctgagaag gatctacaag ctgccctccc tttaccagta tcttcatgca
     9181 tttcctccac ttgatgcaac aagataaagc atcgccattc tgacagcgat tccatttgat
     9241 acctggttaa gtattaccga gtgaacatcg tcggctactt cagaggacaa ctcaactcct
     9301 ctatttattg gtcccggatg cataacaata acatcctcct ttgcaagcct tagcctctcg
     9361 cgtgtaagt
//
LOCUS       DATDRL010000107         6362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_27077, whole genome shotgun sequence.
ACCESSION   DATDRL010000107 DATDRL010000000
VERSION     DATDRL010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27077"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VI935_03560"
     CDS             <1..835
                     /locus_tag="VI935_03560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLE24710.1"
                     /translation="ASLLSLRNRSRLIFFAQGYDEFYYKSPFKKLLIKMVYLFCLKLM
                     KVKTIAVSNQLSKILKEKYDADATTVENGIDSENFYPDCDEDLIRNKDNRKAVLLFSR
                     NDHTKGLNIAIKTLNTLSDEWKDKIEIWICGEEVKKEILKSKAINFGWLGKDKLRKVI
                     SSADVLFYPARHEGFGLFPLEAMACGCSVVTTKAVSYVKDGENALVTEIEDTYNLKEK
                     LKNILNDELLREKLKQSGFETVSKYDLKESQRKFEKVIIDTFPLSHYTSPDKLLKISS
                     K"
     gene            842..2248
                     /locus_tag="VI935_03565"
     CDS             842..2248
                     /locus_tag="VI935_03565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE24711.1"
                     /translation="MKGKILICAYYFPPIGTPRSYRWQHFVTHLYPKGWMIDVLTINT
                     YSNHPNYDSKLLDNLPTEVRIFRTYPGIMHHLSSLLLGKSKSNVIESTFTEPVLREPE
                     VLSVNSARQSDNLQFTSPYTLVSLNQTIGKFLLKIFEKSLRTVFIPDEAVSWLPFALL
                     KGKKLMKKNSYDVIISSGFPFTCHIAGYFLKSLSGGKPWIADYGDPWVNNPSFALPKW
                     RSFIDKEIESRILNSVNKIIVNTDRTKEHYLNLYPFLKSENIKVITQGFSKEGFHEVS
                     PEITDKFRIVYTGIFYKDREPFILFDALTNVKEIWKDLEVIIAGNITKDEYKHYAENK
                     GLSEIVHFIGFVPQEKALSLQKGASVLLLIGNRGGIQVPGKIYEYIASKRPILSITID
                     KYDIASTFVDRFKRGISVENDSQKLSESIIYLYKLWKNKRLESEFNLEDVDEFCWDRL
                     AEKLEETILEATDSNKKN"
     gene            2682..4007
                     /locus_tag="VI935_03570"
     CDS             2682..4007
                     /locus_tag="VI935_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HLE24712.1"
                     /translation="MTTLESLVFRIKSRQFTVAIIGLGYVGLPLVREFARAGFNVLGF
                     DIDEGKVNKLNRGESYISYIFSDTISSLIEGGKFHATSDFSKLAFADAILICVPTPLN
                     HYRDPDLSFVINTTKTISRYLRKGQLTILESTTYPGTTEEEVLPILESSGLKVGRDFF
                     LAYSPEREDPGNKDFATSQIPRVVSGVTPNCLLLAKSLYEQIVSKTVPVSSPKVAEAT
                     KLLENIYRSVNIALVNELKIIFERMGIDIWEVIDSASTKPFGFTPFYPGPGLGGHCIP
                     IDPFYLAWKARRYEIPTRFIELAGEINTNMPYYVVSKISEGLNKNSKSIRLASILLLG
                     ISYKKDIDDTRESPSLKIMSLLKEQGANVEYNDPYVPSISGFRRYPGLKMKSTSLAEG
                     SIERFDCVVIVTDHSCYNWQEIVNRSQLVIDTRNATRVVMSGGEKIIKA"
     gene            4074..4172
                     /locus_tag="VI935_03575"
     CDS             4074..4172
                     /locus_tag="VI935_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLE24713.1"
                     /translation="MTGGAGFISSQLAEALLECGYNVTVIDNLSTG"
     gene            4353..5471
                     /gene="wecB"
                     /locus_tag="VI935_03580"
     CDS             4353..5471
                     /gene="wecB"
                     /locus_tag="VI935_03580"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HLE24714.1"
                     /translation="MRNASKKITLVVGARPNFMKVAPLMHELLKYPEAFHTQLIHTGQ
                     HYDSDMSDIFFDDLELPQPERFLGVGSGTHTEQTAKVMMAFENVCIEEKPDLVVVVGD
                     VNSTMACAITAKKLHIPVAHVEAGLRSRDWTMPEEINRIVTDAISDLLFTPSRDAGNN
                     LLREGIPPERIHFVGNVMIDCLVTHLPKTEKRDTLRRFSLEAGNYATLTLHRPSNVDV
                     KEVLSGILNVILEISEKLPIVWPVHPRSRKMLTELNLLHIVENNQGLILTDPIGYIDM
                     LALNRRARMIITDSGGLQEEATFLGVPCITLRHNTERPVTIEAGSNQVVGNQPAAIRS
                     AIRSILNKKRTEIHIPELWDGRASVRIVDVLMRHYSQG"
     gene            complement(5538..>6362)
                     /locus_tag="VI935_03585"
     CDS             complement(5538..>6362)
                     /locus_tag="VI935_03585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015148917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE24715.1"
                     /translation="ITSFHGYDFSKLINERGSETYKELFRIGDLFTANTNFTKGKLIE
                     LGCPKSKIVMLPVGMDINFFNFRERYLRDRSIVKLLTVARMVEKKGLEYAIKAVSRVA
                     KKYSNIEYNIIGDGILRRRLENLIKELGISDKVNLLGWKTQEELKSLFQDAHIFILTS
                     VTAGDGDMEGQGLVLQEAQAVGLPVLSTLHNGIPEGVIDGKSGFLVPERDVDALANKL
                     KYLIEHPELWPEMGRYGRKFVEERYDIQKLNQRLVKIYEAILAEDSNILESIQTYQ"
BASE COUNT         2003 a         1276 c         1236 g         1847 t
ORIGIN      
        1 agcttcgcta ctgtctttaa gaaaccgctc caggcttatt ttctttgctc aaggttatga
       61 tgaattctac tataaaagcc cttttaaaaa gctacttatt aaaatggtat acctcttttg
      121 tttaaaactt atgaaagtaa aaacaatcgc agtatcaaat cagctttcaa aaatacttaa
      181 agaaaaatat gatgccgatg cgacgactgt tgaaaacggt atagattctg agaactttta
      241 tccagattgt gatgaggatt tgatacgaaa taaagataat agaaaagctg tccttttgtt
      301 ttctagaaac gatcacacaa agggactcaa tattgcaatc aaaaccttaa atacacttag
      361 tgatgaatgg aaagataaaa tcgagatctg gatttgcgga gaagaggtga agaaagaaat
      421 tcttaaatca aaagctatta actttggctg gcttgggaaa gataaactta ggaaggtaat
      481 aagcagcgcc gatgttctct tttatcctgc aagacacgaa gggttcggtc tttttccact
      541 tgaagccatg gcttgtggct gttcagtggt tacaactaaa gccgtttctt atgtaaaaga
      601 cggagagaat gcccttgtaa cagaaataga agatacctat aatttaaaag aaaagcttaa
      661 gaatattctt aatgatgaac tacttagaga aaaattgaag caaagtggat ttgagacggt
      721 ttcaaaatac gaccttaaag aaagccagag aaagtttgaa aaggttatta tagatacctt
      781 ccctttgagc cactatacat cccctgataa gcttctaaaa atttcttcta agtaacaaaa
      841 tatgaaaggg aaaattctta tctgtgctta ttactttcca cccataggca caccgagaag
      901 ttatagatgg caacattttg tcacacatct ttatccgaaa ggctggatga tagatgtact
      961 taccataaat acctacagca accatccaaa ttatgattca aaactcttgg ataatctccc
     1021 gaccgaagta aggatattta gaacatatcc tggaattatg caccatctct ccagtcttct
     1081 tctcggtaag agtaagagta atgtcattga gagcacattc acggagcctg tgctgaggga
     1141 acctgaagtg ctcagtgtaa actccgcgag gcaatctgac aatctacaat ttacttcccc
     1201 ctatactctg gtttccctca accaaaccat tggaaaattc ttattgaaga tttttgagaa
     1261 aagcctcagg acagttttca taccggacga agcagtaagc tggcttccct ttgccctttt
     1321 aaaggggaaa aagcttatga aaaagaacag ttatgatgta atcatttcat ccgggtttcc
     1381 cttcacatgt catatagcag gttactttct aaaatcactt agtggaggca aaccctggat
     1441 agccgactat ggagaccctt gggtgaataa tccctcattt gccttgccaa aatggcgttc
     1501 atttattgac aaagaaatag agtccaggat tttaaattcg gtaaataaga taatcgttaa
     1561 tacagataga acaaaagagc attatttgaa tttgtatcca tttctcaaat cagaaaacat
     1621 aaaggttatc acacaggggt tttctaaaga aggatttcat gaagtctctc cagagataac
     1681 agacaaattt agaattgttt acacagggat attttataaa gacagagagc ctttcattct
     1741 ctttgatgca cttactaatg taaaggaaat ttggaaagac cttgaggtaa ttattgccgg
     1801 aaatataact aaagatgaat ataaacatta cgcagaaaat aaaggcctat cagagattgt
     1861 tcactttatt gggtttgtac cacaggaaaa agccctgtct cttcaaaaag gagcttcagt
     1921 actcctatta ataggcaacc gtggcggaat ccaagtcccc ggaaaaattt atgaatacat
     1981 tgcatccaaa agaccgatac tatcaataac aattgataaa tacgatattg cttcaacatt
     2041 cgttgacagg tttaaacggg gaatctctgt tgaaaatgac tctcaaaagc tgagtgaatc
     2101 aataatttac ctctataaac tgtggaagaa taaacgtctt gaaagcgagt ttaatctcga
     2161 ggatgtagac gaattttgct gggatagatt agctgaaaag cttgaagaaa cgattctaga
     2221 agctacggac tcaaataaga agaattgaat acttatatat tacatcttgc cacttgggaa
     2281 aatggtgaca ttagataggg gcattacgct gctaacagac caaattttat taaaactctt
     2341 cataacgtct gaacaaattt atcagtagtt agtgcctatg atttacctaa attcctgttc
     2401 ctcgatgtag taaatcagat tacctctaga gacagcatga cgcttgagta gatgttcttt
     2461 taaataccac aaaattcttt gacggtgtct gttttattca tagaaatgta cgtaaataat
     2521 gacctcaatt tcgtcctctt tccttgcccc ttgtatagga ggtagttaga gcggagacaa
     2581 gcggagactt ttccttgaca agagattatt ctttgtggac aatgagaata gaaaagattt
     2641 aagtataaga tgagataaaa caaatcaacc aaacaaaaca gatgaccaca ctagaatccc
     2701 tagttttcag gataaaatcc cgccagttta cagtagccat aattggcctt gggtatgtgg
     2761 gactcccgct tgtgagagag tttgcaagag caggctttaa tgtattaggc tttgatattg
     2821 atgaagggaa agtaaataaa ttaaaccgtg gagaaagtta cataagctat attttctcgg
     2881 ataccatttc atctctgata gagggcggga aatttcatgc cacatccgat ttttccaaac
     2941 tagcttttgc agatgccatt ttaatatgtg ttcctacccc tcttaatcat tatagagatc
     3001 cagacctttc ctttgtcata aataccacaa agactatctc taggtatttg agaaaaggac
     3061 agctaactat acttgaaagt accacttatc cggggactac ggaagaagag gtgcttccta
     3121 ttttagagtc tagcgggctt aaagtaggaa gggatttttt ccttgcgtac tctcctgaaa
     3181 gggaagaccc aggaaataag gattttgcta cctctcaaat tcctagagtt gtaagtgggg
     3241 tgacacctaa ctgcctcctt ttagcaaaat cactttatga acaaattgtt tctaaaacgg
     3301 ttcctgtatc ttctccaaag gtagcggaag ctacaaagct tcttgagaat atatacaggt
     3361 ctgtaaatat tgctctggta aacgagctaa agatcatatt tgaacggatg gggatagata
     3421 tatgggaggt aattgactca gcttcaacaa aaccctttgg ctttactccg ttctacccag
     3481 gccccggtct tggagggcat tgtataccaa tagatccttt ctatcttgct tggaaagcaa
     3541 ggcggtatga aattccaacc cgatttatcg agcttgctgg agagatcaac actaatatgc
     3601 cttattatgt ggtgagcaaa atcagcgaag ggctaaataa aaatagtaaa agcataagac
     3661 tagcaagcat tcttcttttg ggtatatctt ataaaaagga catagatgat acgagagaat
     3721 ctccatcact aaagatcatg tcactcttaa aggaacaagg agcaaacgta gagtataacg
     3781 acccatacgt tccttctata tcaggttttc gtcgttatcc ggggcttaaa atgaaatcaa
     3841 catccttagc ggagggttcg atagagagat ttgattgtgt cgttattgtt actgaccact
     3901 cgtgttataa ctggcaggaa attgtgaata ggagccagct cgtgatagat acaaggaacg
     3961 ctacgcgagt tgtgatgagc ggaggagaaa aaattataaa agcctaaatc ctccgtacca
     4021 aatcaagtat atagaccaaa cgaatcacga aatagagtgc gaaatctgct ctaattactg
     4081 gtggtgctgg ctttattagt tcccagttgg ctgaagccct actggagtgt ggctacaatg
     4141 ttacagtcat tgataacctc tccaccgggt agtttaagaa tatttcctac ctgatcaacc
     4201 accctaactt ccgttttggg tacggttaac ctgctggaaa accttttaaa catttcacga
     4261 aaccattata ctatgctccg tgaaatccgt aataccacag ttaatagtat acttagatta
     4321 aagacaaaga gggtttacag caggagagag aagtgcgtaa cgcatcgaaa aaaattactc
     4381 ttgtagtagg tgcaaggccc aattttatga aggtagcacc tctaatgcac gagctgttaa
     4441 agtatcctga ggcatttcac actcagctta tccataccgg tcagcactac gattccgata
     4501 tgtctgatat tttctttgac gaccttgaac ttcctcaacc ggaaagattt cttggcgtag
     4561 gctctgggac ccatacagag caaacagcca aagtaatgat ggccttcgag aatgtttgta
     4621 ttgaagaaaa gccagacctt gttgtagttg tcggggacgt aaactccaca atggcttgtg
     4681 ctattacagc aaaaaaactt cacatccctg tagcacatgt ggaagcgggt cttcgaagcc
     4741 gggattggac aatgcctgag gagataaacc gtatagttac tgacgccatt tctgatcttc
     4801 tctttacacc ctcacgagac gcaggcaata atcttctccg ggaaggtatc cctccggagc
     4861 gaatccactt cgtgggtaat gtgatgattg attgtctggt tacacacctt ccaaaaaccg
     4921 aaaaacgtga tacccttcgg cgattcagcc tggaggcagg caattacgcc actctaacct
     4981 tacacaggcc aagcaacgtt gatgtcaaag aggtcttgag cggtattcta aatgtcatac
     5041 tcgagatatc agagaagctg cctatagtct ggccggttca cccacggtct cgtaagatgt
     5101 tgactgagct taatctttta catatcgtag aaaacaatca gggattaata cttaccgacc
     5161 ctataggtta cattgatatg ctcgccctga atcgcagagc ccgaatgatt atcacggact
     5221 cagggggact gcaggaggag gcaacgtttc tcggtgtacc ctgtatcacc ctccgtcata
     5281 acactgaacg ccctgtaact atagaggccg gttctaatca ggttgtgggg aatcaacctg
     5341 ctgcgatccg ctctgcaatt cgttctatct tgaataaaaa gagaactgaa atccacatcc
     5401 ccgaactttg ggatggcaga gcctcggtgc gcatagttga cgttctgatg cgccactact
     5461 ctcagggtta actctataaa aactgattca atccccgcca tggtcttttc atgataatta
     5521 gattatcgga ttcggtttta ttgatatgtc tgaatactct ccagtatatt gctatcctcg
     5581 gcaaggatag cctcataaat ttttaccaac cgctgattca gtttttggat gtcatatctc
     5641 tcttcaacaa actttcgccc atatcttccc atttccggcc ataattctgg atgttcaata
     5701 agatatttta atttattggc taaggcatcc acatcccgct cagggacaag aaagccggac
     5761 ttgccgtcta taactccttc gggaattcca ttatgtagtg tagataagac agggagtccc
     5821 actgcttgag cttcttgaag gaccaatcct tgcccttcca tatctccatc gcctgctgtt
     5881 acactagtta aaataaaaat gtgcgcgtct tgaaatagac tttttaattc ttcttgtgtc
     5941 ttccagccta atagatttac cttatcgctt atacccaatt ccttaatcaa gttctcaagc
     6001 ctcctcctta atattccatc acctattatg ttatattcaa tgttcgaata cttcttagct
     6061 accctagaga cagccttaat tgcgtactcc agcccctttt tctcgaccat gcgtgctact
     6121 gttaacagtt taactatgct tcgatctctt aagtatcttt ctctaaaatt aaaaaagttg
     6181 atgtccatac ctacaggcag catgacgatt ttactcttag gacaccctaa ttcaatcaat
     6241 ttccctttag taaaattagt attagcggta aaaaggtctc ctattctaaa aagttccttg
     6301 taggtctcac tccccctttc attaattaat ttgctaaaat cgtatccgtg aaaggaagta
     6361 at
//
LOCUS       DATDRL010000108         2239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2427705, whole genome shotgun sequence.
ACCESSION   DATDRL010000108 DATDRL010000000
VERSION     DATDRL010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2427705"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VI935_03590"
     CDS             complement(<1..409)
                     /locus_tag="VI935_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005025975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HLE24716.1"
                     /translation="MGKESSLIVGLDIGTTKVCAIVGEPTYPNGLDIIGVGTYPSFGL
                     KRGVVVNIENTVQSIKHAVEEAEHMAGCEIRTVFAGIAGGHIKGMGGHGMITLRSREV
                     SKRDLDRVIESASALSIPADREIIHVIPQEFNVD"
     gene            complement(501..1205)
                     /locus_tag="VI935_03595"
     CDS             complement(501..1205)
                     /locus_tag="VI935_03595"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HLE24717.1"
                     /translation="MKRKSRFFLTLILLCFLCGAFFLAVLHLDFFTIKEIEVKGPKRV
                     SPKEILKRSGLRPGINMFSFLESSLTEEILKNPWITSANIKRELPGKLIIEIKEIEPF
                     CLVLMENGELYYMSKTGEKLGYANLNDGLDFPVLTGEVIMNPDLLAGALEILKLSLDS
                     PTLNWREISEINLNSVYGLTALTTDGRRIDFGESKIREKWYKVEKIITHARGINLTEK
                     YINISSGKIGVVDFKL"
     gene            complement(1202..2104)
                     /gene="murB"
                     /locus_tag="VI935_03600"
     CDS             complement(1202..2104)
                     /gene="murB"
                     /locus_tag="VI935_03600"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HLE24718.1"
                     /translation="MIRIEKELEKIGCEYKSLFSMSRYTSMRVGGLADLVVYPRSHLE
                     LMTVLNLLTSLNFPWTVLGGGSNTIIGDSGINGVVVSTKKMREIKISGSGKVVAEAGA
                     VLGSVLNKSIRAELSGFEFAAGIPGTVGGGVFMNAGANDGEIKDVIEKVWIWLNGKEI
                     MLSRDELKFEYRKSYLPEGSVVTKAMFGLARGNRKESERRVKDYLDKRSETQPITISN
                     SGSIFKNPPEIPAGMLLEQLGFKGYQIGRARFSEVHANFIVNSGGASAADVLKLIDTA
                     KKEAFLRTGMLLETEVKIIGQEKN"
BASE COUNT          632 a          544 c          339 g          724 t
ORIGIN      
        1 cgtcaacgtt gaattcctga ggaataacat gaatgatttc tctatctgcc ggtatagaaa
       61 gcgcgcttgc cgattctatt acacgatcaa ggtcacgttt ggagacttca cggcttctaa
      121 gcgtaatcat gccatgtcca cccattcctt taatatgtcc tcctgcaatc cctgcaaaaa
      181 cagttcgtat ctcacatcct gccatatgct ccgcttcttc aacagcatgc tttattgact
      241 gaaccgtgtt ttcgatattc acgacgactc ccctttttag accgaaagag gggtaagtcc
      301 caacacctat aatatcgagc ccatttggat acgtgggttc tccaactatc gcacacacct
      361 ttgtggttcc aatatccaag cctacaatta agctggattc tttacccatc ctcctctcca
      421 tacgttttat taattcctat tgtcattaac gcctttacct tatgatttca tcatgcagag
      481 ttaatgtcat ggtatatgca ttaaagctta aaatctacaa caccaatttt ccccgaactt
      541 atatttatgt atttttcagt taaattaatt cctcttgcat gcgttattat tttttctacc
      601 ttataccact tctctcgtat cttgctctca ccaaaatcaa ttcgccgtcc atccgtcgta
      661 agagctgtta atccatagac tgaattcaga ttgatttctg aaatctctct ccaatttagc
      721 gtaggactat caagagaaag ttttaaaatc tctaaagcac ctgctaaaag atcaggattc
      781 attataactt caccagttaa aactggaaaa tcaagtccat cattcaaatt tgcataacca
      841 agtttctctc ctgttttgct catataataa agctctccgt tttccataag aacaagacag
      901 aacggttcga tttcttttat ttcaattatg agctttccag gtaattccct cttaatatta
      961 gcgcttgtaa tccaaggatt tttaagtatc tcctcagtta atgagctttc aagaaaagaa
     1021 aacatgttga taccaggcct aaggcctgac ctttttagaa tctccttcgg agaaaccctt
     1081 ttaggccctt ttacttcaat ttcctttata gtaaaaaagt cgagatgtaa aacggctaga
     1141 aaaaaagcgc cgcataaaaa gcataggaga attaatgtaa gaaaaaatct actttttctt
     1201 ttcaattttt ctcttgtcct atgattttaa cctccgtttc taggagcatg ccagttctta
     1261 aaaaggcctc tttcttagct gtatcaatga gttttaatac atccgctgcg cttgcaccgc
     1321 ctgagtttac aataaagttg gcatgaacct cagagaacct agctctgcca atctgatacc
     1381 ccttaaaacc aagttgttct aaaagcattc ctgcaggaat ttcgggaggg tttttaaata
     1441 tagagccaga attcgagatg gtaatcggtt gtgtttcact tctcttatcc agataatctt
     1501 taacccttct ttcactttcc tttctattac cgcgtgcaag accaaacatt gcttttgtta
     1561 caacactccc ttcgggaaga taactcttac gatattcaaa tttaagttca tctcttgata
     1621 gcataatctc ctttccgttt agccagatcc agaccttttc tataacgtct tttatttctc
     1681 catcgtttgc tccagcattc ataaataccc ccccgcctac agttcccgga attcctgcag
     1741 caaattcaaa acctgaaagc tctgcacgaa tagatttatt taaaactgag cctaatactg
     1801 cgccggcttc tgcaactact tttccactcc ctgatatctt tatctcacgc atctttttcg
     1861 tgctaactac tacaccgttt attccagagt cgccaatgat agtatttgat ccgcctccaa
     1921 gaacggtcca tgggaaatta agagaagtaa gcagattaag aaccgtcata agctcaagat
     1981 ggctcctagg ataaaccact aggtcagcta aaccacctac gcgcatcgat gtgtagcggc
     2041 tcatagagaa aaggctttta tattcacacc cgattttctc aagctctttt tcaatacgga
     2101 tcatgaaagc tcctcgacta ttttttctcc tagcttccac acattgccag ctccaagggt
     2161 aagaactaaa tctccgggtt ttaagacctc caagacacgc tgaattacct catcttgttc
     2221 ctttgcataa tatacattc
//
LOCUS       DATDRL010000109         7416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2682015, whole genome shotgun sequence.
ACCESSION   DATDRL010000109 DATDRL010000000
VERSION     DATDRL010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2682015"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1796)
                     /locus_tag="VI935_03605"
     CDS             complement(105..1796)
                     /locus_tag="VI935_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLE24719.1"
                     /translation="MAKVHGGWLVVKTLYEHGVREIFSLSGGHINPIYDACVDFGIRL
                     IDTHHEQAAAMAADAYGRVKREPGVCLVTAGPGFTNAVTGIAGAYLSNSPCILLSGKS
                     GIEENDRLPLQDIDQQSVITPITKWARTIFDTKRIPEYISTAYKKAISGKPGPVYLGM
                     PYEVLYESCNEEEIDRYNTTLPSYKVEAAREAIAQAIEMLKSARRPVAIAGSGAWYSG
                     ADEELARLLDKVKIPIFTLNFGRGIVSDDHPLCFGAASPSAPVAFKRITSEADVILLL
                     GIRLSLYIGFGRTFNPEAKIIQVDIDPGEIGRNRPADLGIVGDISKVLSQLTDYAEQH
                     SINLNYKSWVEQAKAWREDEWKKVEEIRKSNKTPIHALRVIKEVEDVLGEDGILAIDG
                     GDTQVWADTTYRVTKPGHYVKGGPLGCMGVGVPFAIGTKVACPDKQVALITGDGAIGM
                     NFMEFETAIRHKIPFVTVVCNDQSWGMTKHQLWITYGRERQTVGVDLPLIPFHELVKV
                     LGGYGEFVSEPSQLRGALDRAFSSGVPSLLNVTTDPEAISPATYALTQMMMPKKK"
     gene            1927..2292
                     /locus_tag="VI935_03610"
     CDS             1927..2292
                     /locus_tag="VI935_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24720.1"
                     /translation="MEGQDLGQVSGTMNSVLMAFNLGVSANQQTGGNKSVGEIANELG
                     ETIFSNISQIYGNQFPEEYLRANTEFFLRVALLGYIIPSVCAYDEGLKNRLFALIEAK
                     INQYPQKQDNPQGGTIITT"
     gene            2644..3783
                     /locus_tag="VI935_03615"
     CDS             2644..3783
                     /locus_tag="VI935_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HLE24721.1"
                     /translation="MSKKILGGRDKVKVAVVQASPVFMDKQRTIDKASNLIKEAGRNG
                     AELIAFSEAFIPGYPAYLTVGYETNPHEWTDYMIALQDNSVVIPSKDTEILGQAAKEA
                     GSYVVIGCNELDNRLGSCTVYNSLLFIGKDGNIMGRHRKLMPTYTERLYWGQGDASDI
                     KVFDTDIGRIGGLICWENHMTLVRAAMIHRGEEFHIAVWPGNWKRGETKLLDADTSPG
                     GALCNLQSLIRVHAFEAGAFVLSACGFLTPEDFPKRWHYIRDSDHINYSWALGGSAIV
                     NPAGRFLVEPNFEKDAILYAECYANQIKAVKAVFDSLGHYSRWDVVRLSVRREAWVPE
                     VPLNEPSTSEDELPESELRRISEEFEISVDKLESLLDEIRKIKKN"
     gene            4153..4383
                     /locus_tag="VI935_03620"
     CDS             4153..4383
                     /locus_tag="VI935_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24722.1"
                     /translation="MNFREDYGDYSAQEAVDAILVILKQNGVKIDENSFTWTLVGALF
                     DKGILSRQEFERIYFLLKSQTKESGNSSERNI"
     gene            4800..5783
                     /locus_tag="VI935_03625"
     CDS             4800..5783
                     /locus_tag="VI935_03625"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLE24723.1"
                     /translation="MKVFVTGATGFIGSHLVKRLLRDGASVKALARDSSNVEELKKLG
                     VEIIIGDVTDKISLKGVMKGSDICYHLGNVSRWWLPDKSIYYKINVEGTKNILVEALN
                     EGVKRVVYTSSLAAIRQPKGEIATEEKEHKRDFESHYGRSKFLAEREVLRIYRDYGLP
                     VVILNPGVVIGPGDLKTFGRTIIELLNGKLEAILFEDSMVPLVYIDDTVEGHIIAAEK
                     GKIGDKYILVGDNIKFGEVFKLINRITGVPLPQKRISPYLIKIIAYISELKSIWTGKS
                     PRFAQDAIRAMEIGAIGENKKAREELGVHFTPLEEGLRKTIEWYKENGYVH"
     gene            6074..7408
                     /gene="ffh"
                     /locus_tag="VI935_03630"
     CDS             6074..7408
                     /gene="ffh"
                     /locus_tag="VI935_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HLE24724.1"
                     /translation="MFEGISEKLSSTLKKIRGYGKLSENNIQDALREVRLSLLEADVN
                     FKVVKDFIESVKNRALGQEVIESLTPGQQFIKIVHEEIVRILGEESSDLDLRATPPVA
                     IMLVGLQGSGKTTTAAKLAKLLKENLKRKPYLVPADVYRPAAIEQLKVLAQTIGVGVY
                     DSQQGNNPVDICKEAVSSAIYGSYDVAIIDTAGRLHIDEELMDELRRIKAEIRPHEIL
                     LVADAMTGQDAVNVATSFNEALDIDGIILTKMDGDARGGAALSIKAVTGKPIKFFGVG
                     EKLEALEVFHPDRIASRIIGMGDVLTFIEKAQVAFEEKKAEEMTKKLLRNEFLLEDFR
                     EAMIAIRKMGALDSMTEMIPGMKQIAKNPKALEMAEKEIKKTIAIIDSMTLKERKSHT
                     ILNGSRRKRIAKGSGTKVEDINRVIKNYVQMRDMMKNMGKMGKLAKRMMRYM"
BASE COUNT         2319 a         1457 c         1586 g         2054 t
ORIGIN      
        1 cccgcctatc ttttccaccc tttaaaaatg atggagttaa gtccccctta attataaagg
       61 gggattttaa gtggagaatc taatcatccc taacccctac caaatcattt cttcttcggc
      121 atcatcatct gggtaagtgc ataagtcgcc ggacttattg cctctgggtc tgttgtaacg
      181 ttcagaagcg aaggaacacc agaagaaaag gcacggtcta acgctccacg aagctggcta
      241 ggttctgaca cgaattctcc atatccacct aaaaccttca ccaattcatg aaaaggaatt
      301 aggggaagat caactccaac cgtctgacgt tctctcccat aggttatcca gagctgatgc
      361 tttgtcatac cccaggattg atcgttgcag acaacggtta caaaaggaat tttatgtctt
      421 attgccgttt caaactccat aaagttcatc cctattgcgc cgtcccccgt aataagcgca
      481 acctgcttgt ctggacaagc cactttcgtc cctatcgcga aaggaacacc tactcccata
      541 cagccaagag ggccaccctt tacgtaatgt ccgggctttg tcacacgata agtggtgtct
      601 gcccagacct gtgtgtcgcc gccgtctatt gcaagaattc catcttcccc tagcacatct
      661 tctacctcct tgataaccct gagtgcatgt attggggttt tattagactt tcttatctct
      721 tctaccttct tccactcatc ttcgcgccat gcttttgcct gttctaccca tgatttgtaa
      781 tttagattta ttgaatgctg ttctgcataa tctgtgagtt gtgatagcac cttgcttata
      841 tcacctacta tccctaaatc tgctggtctg tttctaccga tttctcctgg gtcaatatct
      901 acctgaataa tctttgcttc ggggttaaag gttcttccaa accctatata gaggcttagc
      961 ctgatcccta aaagcaaaat tacatcggcc tcagaggtta ttctcttgaa ggcaacagga
     1021 gcagaaggac tagcagcacc gaagcataat ggatggtcgt ctgagacgat tccccgccca
     1081 aaatttagag tgaatatcgg aatcttaacc ttatctaata atctcgccag ttcctcatcc
     1141 gcacctgaat accatgcgcc gcttccggcg attgctacgg gtctacgcgc tgatttaagc
     1201 atctctatag cctgcgctat agcttctctt gcagcttcta ctttgtatga gggaagagtt
     1261 gtattataac gatctatttc ctcttcattg cagctttcgt atagaacctc gtatggcatt
     1321 cccaaataaa cgggtccggg ttttccagaa atcgcttttt tatacgctgt gcttatgtat
     1381 tcaggtattc gctttgtatc aaagatagtt cttgcccatt tcgttatggg tgttattact
     1441 gattgctgat caatgtcttg taaagggagc ctgtcgtttt cctcgattcc cgacttccca
     1501 gacagtagga tacaaggaga atttgagaga taagctccgg ctatccctgt aacggcgtta
     1561 gtgaatcctg gtcctgccgt tactaaacac acccctggct ccctttttac ccttccatat
     1621 gcatctgctg ccattgcagc cgcttgctcg tgatgagtat caataagcct tataccaaaa
     1681 tccacacatg catcataaat ggggtttata tgacctccgc ttagtgagaa tatttcgcgc
     1741 acgccatgct catatagggt ttttaccaca agccatccac cgtgaacctt agccatcttt
     1801 gcctccttgc caatctctaa ttatacttta ttacactcta catcctaaca aatcaaatat
     1861 taaaaaagta gaatctattg aagacaatta taatactgat agaattaaaa aaaggaggaa
     1921 agaaaaatgg aaggtcaaga tcttggtcaa gtaagcggta cgatgaattc ggtattgatg
     1981 gcgttcaacc ttggtgtttc tgccaatcag caaacagggg gaaataaaag tgtaggtgag
     2041 attgcaaatg agcttgggga gacaattttt tccaacattt ctcaaattta tggtaatcaa
     2101 tttccagagg aatatcttcg tgctaacact gaatttttcc ttcgagttgc ccttttggga
     2161 tatataattc ctagtgtatg cgcatatgac gaagggctca aaaaccggct ttttgcacta
     2221 attgaagcta aaattaatca ataccctcag aaacaagaca atccgcaggg tggaactatt
     2281 atcacaacct aaagaattat gaatcgtggt tagttaatag tgattcgtga ctggttcttc
     2341 caatacctga ttatttggca ataaccgttt tactcataac tgatgattgt ttaccggttt
     2401 ggaaatttac ctaatcaact atcccgcttc gtctgataaa tcgtatttgc tggttcaata
     2461 acctcagtag actcctatat aagaagagct tcactagggt gtaaaatcat taaaaccctg
     2521 cccacacatt gctgcctacc ctaattttgc tttctattcc caattaacct tcttttatgt
     2581 cttaaatttg agcataatat cacttataat taataaaccc cgcttaaagc tacgaggtca
     2641 aaaatgagca aaaaaatcct aggaggtaga gataaagtaa aggtagccgt cgtgcaggct
     2701 tcaccagtgt tcatggacaa gcaaaggact attgataaag cctccaattt aataaaggag
     2761 gcgggaagaa acggtgcaga gcttattgca ttttcagaag cctttatccc tggctatcct
     2821 gcatacctta cagttggtta cgaaacaaac ccgcatgaat ggactgacta tatgattgcg
     2881 cttcaagaca actcagttgt gattccaagt aaagacacag agattttggg acaagctgca
     2941 aaggaggctg gatcctacgt tgttattggt tgtaacgagt tagataaccg tcttgggagc
     3001 tgtacagttt acaactcgct tctttttatt ggaaaagacg gcaacatcat gggaaggcat
     3061 agaaaactaa tgcccacata cacagagaga ctctattggg gccagggtga tgcaagcgac
     3121 atcaaagtat ttgatacaga tattggtcga atcggagggc taatatgctg ggaaaaccac
     3181 atgacactcg taagagccgc aatgattcat agaggagaag agtttcatat tgcagtctgg
     3241 cctggaaact ggaaaagggg tgaaactaag ctactcgatg ctgatacaag tccgggagga
     3301 gcactctgta atcttcagtc acttataaga gtgcatgctt ttgaggcagg cgcctttgtc
     3361 cttagtgcat gcggtttttt aaccccggaa gactttccta aaaggtggca ttacattagg
     3421 gatagcgatc atataaacta tagttgggca ttgggtggga gcgctattgt aaaccctgca
     3481 gggagattct tggttgagcc taattttgaa aaggacgcga ttctttatgc cgagtgttat
     3541 gcaaatcaaa taaaggctgt aaaagccgtt ttcgattcac ttggtcatta ctcaagatgg
     3601 gatgttgtac gcctttctgt gaggcgcgaa gcctgggttc ccgaggttcc tttaaacgag
     3661 ccatcaacct ccgaggatga gttgcctgaa agcgagctta gaaggatttc cgaagagttt
     3721 gaaataagtg tagataaatt agaatcgcta cttgatgaga taagaaaaat aaaaaagaac
     3781 taagcattaa cttgaaccaa atattgaatt aaagaaagtc ctaaccgtca ttgcaatgac
     3841 agagaaaacc ctgtagtaag caagctacag agaattatca agttaaaagt tctatcttta
     3901 gaatggctta tgctgcggtg atagtttgga ataagtataa aagcctcatc tttcgctaaa
     3961 ctcaaagcat cttaagtaaa aaagcaacca atttggtttt aattaccgtc taaaaaatag
     4021 tgacattatc actttgcaac aacacatcta aaaataggac ttgacaagca ttccaataaa
     4081 ttatataaac ctaagcatta ggggacatag tattttaact gcataattag cctatcttaa
     4141 cggaggatga agatgaattt cagggaagat tatggagatt actcagctca ggaggctgta
     4201 gatgctatac tagtaatcct caaacaaaat ggcgttaaaa ttgatgaaaa cagcttcact
     4261 tggacattgg tcggggccct ttttgataaa gggatactct cccgtcagga atttgaacgt
     4321 atatactttc tgcttaagag tcaaacgaag gaaagtggta attcttcaga aagaaatatt
     4381 tagatcttaa agtctaatct gaccaatctt gtatcatcgg cttttcatat ttaccttaca
     4441 caattaataa ctaatggaat accttgcggc aacctgctat cggcatataa agagacattc
     4501 ccttttgtta ttgcgaggag ctcttcccac ggtttaaccg ggggggcttc ggtcgggact
     4561 tcgtcgtgag ctcagtaaaa cgataaactc catgacaagc aatctgatac tggatgacaa
     4621 atcgggccgt cattgcgatg actgtggaaa ccctggagtt agctatacgg aactgtttag
     4681 ttgaaaatcc acttgcagac tctgaataat tcgcaaaagt ttttcaaagc agagtttgat
     4741 aatggaaaaa atttatactt attatagact ctaacatatt caaacacgga ggtgaacttt
     4801 tgaaggtatt tgtaacagga gctacaggat ttataggaag tcatttagta aaaagactcc
     4861 ttcgagatgg ggcaagtgtc aaggcccttg ctagagattc tagtaatgta gaggaattaa
     4921 agaaactcgg agtagaaatt ataatagggg atgtgacgga caaaatatcg cttaaaggtg
     4981 ttatgaaagg ctctgatatt tgttatcacc tcggtaacgt ttcccgctgg tggctcccag
     5041 ataaaagcat ctattataaa atcaacgttg agggtacgaa aaacatattg gttgaagcgc
     5101 ttaacgaagg tgttaaaagg gtcgtttaca ctagcagtct tgccgctata agacaaccta
     5161 agggagaaat tgccacagaa gagaaagaac acaaaaggga ttttgagagc cattatggaa
     5221 ggtcaaagtt tttggctgaa agggaagtgc ttagaattta tagagattac ggacttccag
     5281 ttgtaatttt aaacccgggt gttgtgattg ggccagggga cctaaaaacc tttggaagaa
     5341 caattataga acttcttaat ggaaagcttg aagctatatt gtttgaagat agcatggttc
     5401 cacttgttta tattgatgac actgtagagg gtcacataat tgcagcggaa aagggaaaaa
     5461 tcggagataa atacatactg gttggggata acatcaagtt cggagaagtt ttcaagctca
     5521 taaacagaat cacaggagtt cctctaccac aaaagcgaat atcaccatat ttaattaaga
     5581 taatcgcata tatttcagag ctaaaatcaa tctggacagg aaaatcacca aggtttgctc
     5641 aagatgcaat tagagcaatg gagatcggag caattggaga aaataaaaaa gcgagagaag
     5701 aattgggagt gcattttaca ccgctagagg aagggcttag aaaaacgatt gaatggtata
     5761 aagaaaatgg atatgtacat taaccatggt agaactaacc atttttgagc gccagaaaac
     5821 tatgtccaat ggcacttcca ttactccgta ttaccgatca ggcaataaaa tgtttctaat
     5881 ttaaaatctt gggattgcat taataacccc ccgtaaaatt attttctgct ctgcaggttg
     5941 tttcgatcct agaaacctag cgcatacaat atgtaaacac cacatttaaa caccgcgctt
     6001 tatttgacat tggacgtagc ttatgctaac tattaacatt ctcatgaggc aggagtagat
     6061 tacctaagcg gctatgtttg aggggatttc tgaaaagcta agttcaactt taaagaaaat
     6121 aagaggctat ggaaaactaa gcgaaaataa tatccaggat gcacttcgtg aggtccgtct
     6181 tagtctgctt gaagcagatg taaactttaa ggtagtcaag gattttatag aatcagttaa
     6241 aaatcgagct ctcggtcaag aagtgattga gagcctcacc cccggccaac agttcataaa
     6301 aatcgtccac gaagagattg tcagaatctt aggagaagaa agctctgatc tcgacctcag
     6361 ggctactccc cccgtagcta taatgctagt aggacttcag ggctcaggaa aaaccacaac
     6421 cgctgcaaag ctagcaaaat tacttaaaga gaatctgaaa aggaagccat accttgttcc
     6481 tgcagatgtt tatagacctg cggctataga acagcttaag gtgctagctc aaacaattgg
     6541 agttggtgtt tacgactctc agcaaggaaa taatccagtt gatatttgta aggaagctgt
     6601 ttcttcagcg atctacggca gctacgatgt cgcaataatt gacactgcag gaagacttca
     6661 tattgatgaa gaactcatgg atgaactacg aaggataaaa gctgagataa ggcctcatga
     6721 aatactcctt gttgccgatg caatgacagg tcaggatgct gtgaatgtcg caactagttt
     6781 taacgaagct ctcgatatcg atggaataat acttacaaaa atggatggcg atgcgagagg
     6841 cggggctgcc ctttcgatta aggccgtaac cggaaaacct ataaagttct ttggagtcgg
     6901 agaaaaacta gaggctctag aggtgtttca tcccgatagg attgcctccc gaattatagg
     6961 aatgggcgat gttctcactt ttattgagaa ggcccaagtc gccttcgaag agaaaaaagc
     7021 ggaggagatg acaaaaaagc ttctaaggaa tgagttctta ttagaagatt ttagagaagc
     7081 tatgattgcg atacgtaaga tgggcgctct agatagcatg acagaaatga ttcctgggat
     7141 gaagcagatt gctaaaaacc ctaaggccct agagatggcc gagaaagaga ttaagaaaac
     7201 gattgccatt attgattcta tgacccttaa agagagaaag agtcatacga tattaaacgg
     7261 aagcaggcgc aaacggatcg caaagggaag cggtacaaag gttgaagaca taaaccgtgt
     7321 tattaaaaac tacgtgcaga tgcgtgatat gatgaaaaac atgggaaaaa tgggaaagct
     7381 tgcgaagaga atgatgaggt atatgtagct aatgaa
//
LOCUS       DATDRL010000110         5103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_528650, whole genome shotgun sequence.
ACCESSION   DATDRL010000110 DATDRL010000000
VERSION     DATDRL010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528650"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VI935_03635"
     CDS             <1..321
                     /locus_tag="VI935_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HLE24725.1"
                     /translation="AVSIVAFSFSKIFTEAIESKQTAEQLALKARRDLDRIKIEAEQK
                     ITAAQAEAESLRLQRANISTDLIELRKIEANLKAIEKWNGILPQVTGGGAVPFIGVGE
                     MQKR"
     gene            825..1697
                     /locus_tag="VI935_03640"
     CDS             825..1697
                     /locus_tag="VI935_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24726.1"
                     /translation="MNFYLTIIIPAPHSKLFILTAIFSLIVFSFCASVHAHPVADDVV
                     DELNQRIKKDPKNAELYLKRGVLLRESKRWDEAVVDFELASKLDSELKVVDLAKGQLF
                     LEAGWPKAAEFYLRLYLSVSPQNPKALLLLARSLRDQGLGLEAGNTYTEFLQLTKNPK
                     PDYYLERAEALLSVDKDSDALIGLDEGLARFGPITSLQQMALEIDLENKNYDSSLNRL
                     NLLVKNSPRKEYWLLRRGEILEKIGRKAEAKKSYEEALKNLKSRPAHRRNLPVLVKLE
                     KEIREAVARIERGI"
     gene            1756..4380
                     /locus_tag="VI935_03645"
     CDS             1756..4380
                     /locus_tag="VI935_03645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HLE24727.1"
                     /translation="MYKRQLKNILCEAKAIKIFIFIFLGVLFQTTVVASSEVILVSRG
                     SVWKYLDNGSNQGTGWILPSFNDSSWSSGPAELGYGDGDEATVVSFGPDAGNKFTTTY
                     FRRSFQVNNAGSYFNLTLRLKRDDGAVAYLNGTEVFRSNMPSGQIFFNTFASSAIGGT
                     DETTFFETSVNPSLLLEGTNLLAVEVHQANLTSSDISFDLELVASDGSIQLTRGPYLQ
                     MGTQNAVTIRWRTNVPSTSQVRYGTSPTNLEFVNDYPNSVTEHQVRLTGLTPDTRYYY
                     SIGTIDEVLAGGDADHNFVTSPPVGSSKATRIWIIGDSGTADANAQAVRDAYQSFNSG
                     QDTGLWLMLGDNAYEDGTDSEYQAAVFGMYPDLLRKSVVWPTLGNHDGHTADSQTETG
                     PYFNVFTLPGQGEAGGVASGTEAYYSFDYGNIHFICLDSYDSDRSPTGAMMTWLEDDL
                     SQTNANWIIAFWHHPPYSKGSHNSDTETELIEMRENALPILEMYGVDLVLSGHSHSYE
                     RSFLIDGHYGTSDTLQPSMILDSGDGRTDGSGAYEKPALGSSPHDGSVYVVNGSSGRL
                     SSGLLNHPVMFISLLQLGSLILDINGNIMNAQFLNSNGQISDHFTIVKGTAGPVCGNG
                     IKESGEECDGTDVGGATCGNFGCSGGTLSCTGSCTLDSSTCTGCTVCDNDGVCEVGED
                     CNSCPNDCISGTTASCGNGLCEAGDGEDCLSCPQDCRGKQGGKPSGRFCCGDGDGQNP
                     LSCTNPTCTSNGFSCTDIPTTPSCCGDSTCEGIEDSLNCEIDCGPPPFCGDAFCDQNE
                     SSCSCPIDCGQPPITEISCNDGIDNDCDLLVDCNDSNCSGNPVCSPSCFSQGASCTTN
                     SQCCSNKCKGSPGQKVCN"
     gene            4631..4942
                     /locus_tag="VI935_03650"
     CDS             4631..4942
                     /locus_tag="VI935_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24728.1"
                     /translation="MGVAVGSIFHIQLGQATLDKLLTKLGISHIEQLNLFNDALCGTS
                     IQLQQALDQINPQMREQVNNGLNQAARASFSSVMLVGAIIALIGAEVSLWLLKPSKFV
                     R"
BASE COUNT         1536 a         1030 c         1155 g         1382 t
ORIGIN      
        1 gcggtttcta ttgtcgcatt cagcttctct aaaatattta cggaggctat tgagtcaaag
       61 cagacagcag aacagcttgc actaaaggca aggagagacc tggataggat aaagatagag
      121 gcagagcaga agattacggc ggcacaggca gaggcagagt ccttgaggct gcagagagca
      181 aatatttcaa cagatcttat agaacttaga aagatagagg ccaatctcaa ggctatagaa
      241 aaatggaatg ggatactccc ccaggttaca ggtggcggcg cagtgccatt tattggcgta
      301 ggtgagatgc agaagagata accgagtaat cattggcggt atccattggt tagatatgtt
      361 attctgctta ctatcgcagg tattgtgggg agcataatag ctataagaaa agggcacagc
      421 ccgatattgc aacaagagaa gggggtcagg tctcaatttt caacttatct ctacaaaatt
      481 tttactaacc atatgttttt cttgtaatgt caaaaattga cgggctgttg cccaaatcct
      541 aacttgatac aaagttgatt aacaatcgaa atgaagagac aataaaatca ataacttaga
      601 aagatgaaaa gttgatgttt ttcccataaa tgtcctaaaa agctatttga gcaacagcct
      661 gtcaacattt gacattacta cagcaagtta gatggttaga aaaacatttg aaaagagaaa
      721 agttgaaagt tgagacctga ccccatcttt catcttcttg cacaaaataa gacctgacat
      781 tattctatta ttattttcaa atgacaggga tgcgtttaga tcaattgaat ttctacctta
      841 cgataataat tccagcgcca cattcaaaac tctttatttt gacagcgata ttttctttaa
      901 ttgttttctc cttctgtgct agtgtccatg cgcaccctgt agcggatgat gttgtagatg
      961 aactgaacca aagaatcaaa aaagatccaa aaaacgcaga gctctatctc aaaagaggcg
     1021 tgttgttaag agaatctaaa agatgggatg aggcagtcgt tgattttgag ctcgcatcta
     1081 agctagatag cgaactcaag gtcgtagacc tagcaaaagg gcagcttttc cttgaagccg
     1141 gatggcctaa agcagcggaa ttttacctca ggctttatct atcagtgtca ccacaaaatc
     1201 caaaggcgct acttttgctg gcgcgtagcc ttagagatca aggacttggt ttagaagcag
     1261 gtaacacata tactgaattt ctacaattaa ctaaaaaccc aaagccggat tattacttag
     1321 aaagagccga agcactctta tctgttgata aggattcaga tgcgctaata gggctcgatg
     1381 aaggtctcgc cagattcgga ccaattactt cattgcaaca aatggcacta gaaatcgatc
     1441 ttgagaataa aaactacgat tcatcattaa atagattaaa tttattagtc aaaaattctc
     1501 ctcggaagga gtattggctt ttacgtcgtg gagagatatt agagaaaatt ggccgtaagg
     1561 ctgaggccaa aaaatcttat gaggaagcat tgaaaaatct caagtcgcgc cccgctcata
     1621 gacgaaacct acctgttctt gttaaattag aaaaggaaat acgagaagcc gtagcacgga
     1681 tagaaagagg gatataatag acctcttaat acagattttg tttattttca aaatcctttg
     1741 accggaggat ggcatgtgta taaaagacag ttaaagaaca tactgtgtga ggcaaaggca
     1801 ataaagattt ttatctttat atttttaggc gtattattcc aaactacagt tgtagcttcc
     1861 tcagaagtca ttttggtgtc acggggttcg gtttggaaat atcttgacaa cggatccaat
     1921 caggggacag gttggatatt acctagcttt aatgattcca gttggtcgtc tgggccagct
     1981 gaactcgggt atggggacgg agatgaggca acagttgtaa gcttcggtcc agatgccggc
     2041 aacaaattta ccaccactta ttttcgtcgt agcttccagg tgaataacgc cggaagctat
     2101 tttaacctta ccctccgtct caaaagagac gatggggctg tagcatatct caatggaacc
     2161 gaggtatttc gtagcaatat gccttcggga caaatatttt ttaacacatt cgcatcctca
     2221 gcaataggag gaacagatga aactaccttc tttgaaacct cagtaaatcc atcgttattg
     2281 ttagaaggga ccaatctact agctgtcgaa gttcatcagg ccaatctcac aagttcagat
     2341 attagcttcg accttgagct cgtagcttca gatggctcta tccaactaac tcgtggccct
     2401 tacctccaga tgggaacaca gaatgctgtt accattcggt ggcgcactaa cgttccatct
     2461 actagtcaag tgcgatacgg aacaagtccc actaacttgg agtttgtaaa tgattatcca
     2521 aactcagtta ctgaacacca agtgcgatta accggattga ctccagatac aagatactat
     2581 tattcaattg gaactattga cgaggtgcta gccggaggtg acgcagacca caatttcgta
     2641 acatcccctc ctgttggctc atctaaagca actcgtattt ggataatcgg tgattcaggg
     2701 acagcggatg ctaacgcgca agctgtgcgt gatgcctatc aaagctttaa tagcggtcaa
     2761 gacactgggc tttggctcat gttgggagac aacgcttatg aagatgggac agactcggaa
     2821 taccaagcgg cggtgtttgg catgtatcct gacctcctcc gtaaaagtgt agtttggcca
     2881 acactaggaa atcacgatgg ccacacagcg gactcgcaaa ccgaaaccgg tccctacttt
     2941 aacgtcttta cactcccagg acagggagaa gcagggggtg tagcttcagg tactgaagct
     3001 tattactcct ttgactacgg gaacattcat ttcatatgtt tggattcata tgacagtgac
     3061 cgttccccaa ctggagcaat gatgacatgg ctcgaagacg acctttcaca aactaatgcc
     3121 aactggatta ttgcattctg gcaccatcca ccatattcaa agggctcaca taactccgat
     3181 acggaaaccg agttgataga gatgagggaa aatgcccttc ccattcttga gatgtacggc
     3241 gtagatttag tcctttccgg acatagccat tcctatgaga gaagcttcct aattgatgga
     3301 cactacggaa cctctgatac acttcagccc tcgatgattt tggactcagg cgatgggcgc
     3361 actgatggaa gtggcgccta cgaaaaacca gcccttggtt cttcgcccca tgatggatct
     3421 gtttatgttg tgaatggaag ttcagggaga ctaagcagcg gtctactgaa tcacccagtc
     3481 atgtttatat cacttcttca attagggtca ttgatcctcg atataaatgg gaatatcatg
     3541 aacgcccagt ttttaaacag caacggtcaa atctcagatc acttcacaat tgtaaaggga
     3601 acagcaggac cggtttgcgg aaatggtatt aaagagtcgg gagaagaatg tgatggcaca
     3661 gacgtcggtg gtgctacttg cggtaatttt ggttgttctg ggggcacttt gagttgcaca
     3721 ggatcatgca ctcttgacag ttcaacctgt actggttgca ctgtgtgtga taacgacggt
     3781 gtgtgtgagg taggcgagga ttgtaattcc tgtcccaatg attgtatatc aggtactact
     3841 gcctcatgtg gaaatgggct gtgcgaggca ggcgatggcg aagactgttt atcctgtcct
     3901 caggattgcc gcgggaaaca gggtggtaaa ccctcaggtc ggttctgttg cggcgacggc
     3961 gatgggcaaa atcctctttc ttgcaccaat ccgacttgta cctcaaatgg cttttcttgc
     4021 actgatattc ctacaacgcc ttcatgttgt ggagactcaa catgtgaagg aattgaagac
     4081 agcctaaatt gtgagattga ctgtggacct cctccgtttt gtggagatgc tttctgtgat
     4141 cagaacgaat cctcctgttc ttgtccgatt gactgcggac aacctccgat tactgagatt
     4201 agctgtaatg atggaataga taacgactgc gatttgctgg ttgattgcaa cgatagcaac
     4261 tgcagtggta atccggtatg ctcaccgagc tgtttttcac aaggtgcttc atgcactaca
     4321 aactcgcaat gttgctcaaa caagtgtaaa ggaagtcctg gtcaaaaagt ctgcaattaa
     4381 gtaacggtta gacctcaaaa caacaaaatc ggggtcacct ataggggtga ccctaacatc
     4441 tgagtagcaa gtgaactatt caacgtattt acagactcaa gccctattat tatatcaata
     4501 aattttcgtc aaaaaaggcg acttaatttg acatcttttc cacttatcgg tgaatcaaaa
     4561 ctgctaggac tactagattg gtataagatc aaggccccaa gggacaaact ttgatttctt
     4621 aggggcaact ttgggcgttg cagtaggaag catatttcat atccaattag gtcaagcaac
     4681 cttggataaa ttactaacga agcttggtat ctcgcacatt gagcaattaa atcttttcaa
     4741 tgatgcattg tgtggtacga gcatacagct tcagcaggcc ctagaccaaa tcaatccgca
     4801 aatgagagaa caggtgaata atggcctaaa tcaggcggct agagcaagtt tttcaagtgt
     4861 catgttggtt ggtgctatca ttgccttaat cggcgctgaa gttagtttgt ggctacttaa
     4921 accaagtaaa tttgtacgat agcatgacag atatgaaagt tgaaaaaaac aatatcgaag
     4981 taattactca agatgagggt tcacgccaaa atcagattta taccggcttt ctaatttgaa
     5041 acatctttcc tggaagctga gtaactaggc cactaagttc taaagataga agaattgtaa
     5101 gtg
//
LOCUS       DATDRL010000111        25986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_149813, whole genome shotgun sequence.
ACCESSION   DATDRL010000111 DATDRL010000000
VERSION     DATDRL010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 25986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25986
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149813"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1354
                     /locus_tag="VI935_03655"
     CDS             59..1354
                     /locus_tag="VI935_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24729.1"
                     /translation="MKKLILSLIITFLIIFPPTLRAQNFSSGGFSKGGFRGGGFTRTG
                     GVSKISGFSFRGGFSNSAATSNTSGMGSKNGNSRGFTRSNFNRGGLSPNLSNKPNARL
                     FKNTANIQRPSISTGSSNNNLPTLHIPEGAIVKSVKSEPKNISPNLHTKSGDFRSNSV
                     KNGIIAKEDRNIALRDNLNSTTKGRTNPFTNGRTRSQLMSRGLNSNTLNYNRISNIKN
                     VNVSPNSIINRVPPRKGISPIQNDLSNTPSRNAEIAHWVDNGVMHFTNRVSSLPNGVK
                     TVNVNNGNNSVPVSRDLNADRNLSTSGPLINSLSTNSKNPVRLSMKQNTNSRHSKNGF
                     VNDNIHVSTNNVFNFSFNFFFPSPFFFQPFFFPALFDPFVFNPLFPSPFFTFNPLIPP
                     PVIDPFLFQPFFVSPFFNPFPFSTVFFINTSVSNDVIID"
     gene            1489..1917
                     /gene="rplM"
                     /locus_tag="VI935_03660"
     CDS             1489..1917
                     /gene="rplM"
                     /locus_tag="VI935_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HLE24730.1"
                     /translation="MKTYMARSEDIQKKWYLIDANGKTVGRLATKIATILRGKGKPEF
                     TPHADIGDFVIVINADRVHFTGKKWSKKEYYWHTPYPGGLKSITAEKLFKEKPEEILQ
                     KAVWGMLPKTKWQKKLITRLKIYSGNEHPHIVQNPEKLEA"
     gene            1927..2319
                     /gene="rpsI"
                     /locus_tag="VI935_03665"
     CDS             1927..2319
                     /gene="rpsI"
                     /locus_tag="VI935_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002833359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HLE24731.1"
                     /translation="MAEITYHGTGRRKTSVARVLLRTGDGKISINDRPVEFYFPREAW
                     RIHALEPLQVTQTSGKFDVIVDVSGGGLRGQSGAIRHGLARALLHVNEAFRKALKSAG
                     LLTRDPRMVESKKYGRRKARRGQQYSKR"
     gene            2525..3841
                     /locus_tag="VI935_03670"
     CDS             2525..3841
                     /locus_tag="VI935_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HLE24732.1"
                     /translation="MEIIPPQGRLGVLLPGLGAVSTTFIAGIEAIKRGLGKPFGSLTQ
                     MGTIRLGKRTEKRSPLIKDFVPLVNPEDFVFASWDIFPDNCYEAALKSGVLEKNLLEE
                     LRFELEEIEPMPAVFDRRYVRNLKGIHLKKEKTKMQLAERLIDDITNFKKENKLDRLT
                     MVWCGSTEIYIKPSKVHSTIQNFEEGLKDNHTEIAPSMIYAYAAIQCGVPYANGAPNL
                     SADIPALINLAKRKKVPLAGKDFKTGQTLMKTIIAPGLKARMLGLSGWFSTNILGNRD
                     GEVLDDPYSFKTKEESKLSVLEYILQPDLYPELYSDYYHKVRINYYPPRGDAKEAWDN
                     IDIFGWLGYPMQIKINFLCRDSILAAPIVLDLVLFLDLARRAGLYGIQEWLSFYFKSP
                     MFGEGLYPEHDLFIQLMKLKNTLRYLKGEELITHLGLDYYDYYEPG"
     gene            complement(3976..4482)
                     /locus_tag="VI935_03675"
     CDS             complement(3976..4482)
                     /locus_tag="VI935_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24733.1"
                     /translation="MSKNDSELKDIKIISADEEISQLSRIIEEHKVFWITGPIELPDQ
                     KSGRRVQVALALVISGTYEETPHDTEIPNVFQKLKRIADWLIPKDNPNVRFEIKIHDS
                     RSFYIPGDKDTDRRNYALGIQIWHSEGFDRPVDKHQIEVLKEMEEKLKKIGSHKDHWK
                     DSSKERID"
     gene            complement(4590..5366)
                     /gene="fabI"
                     /locus_tag="VI935_03680"
     CDS             complement(4590..5366)
                     /gene="fabI"
                     /locus_tag="VI935_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HLE24734.1"
                     /translation="MFDSDQIMLKGKNALVIGIANENSIAYGCAKAFKFRGAAVAVTY
                     GNEKSKPYVEPLAKELGAPIFMSCDVTKEGELEAVFSEIERTWGKLDIALHSIAFAPK
                     KDLQGRLVDSSREGFLLAMDVSCHSFIRMARLAEPLMKDGGTLFTMSYYGAQKVVQHY
                     NLMGPIKAALEASVRYLAAELGPKIRVHAISPGPIKTRAASGLADFDDLLNIAAAKAP
                     AKRLVTIDDVGAATAGLATDAAKLITGETIYIDGGYHIID"
     gene            complement(5394..6590)
                     /locus_tag="VI935_03685"
     CDS             complement(5394..6590)
                     /locus_tag="VI935_03685"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HLE24735.1"
                     /translation="MKNLILILNAGSSSLKFSVFSLDSGADLGVFLKGQIEGIGTEPR
                     FIVKDSDGATIAEEHWSAAEVSNQADSLERLAGWLRTNRPGYPLAAVGHRVVHGGPDF
                     ASPAVVNDEVMAKLESFVPLAPLHQPHNLAAIRAVTRYNPRLPQVACFDTSFHRTQPK
                     VAELFGLPYEYYDKGIRRYGFHGLSYEYISKRLKTISPRIASGRAVVAHLGSGASMCA
                     MKDGRSVATTMGFTAVGGLPMGTRCGNLDPGVIIYLVRERGFSIDKVEDLLYKRSGLL
                     GLSGVSNDMRRLLSSTDPRAAEAVDYFIYRVACELGSLVSAMGGLDALVFTAGIGENS
                     SEIRSRICARSAWLGIHIDEVANKSNKLCITKPDSPVSVWVVPTNEEYVIARHTMEAL
                     GLDKTQ"
     gene            complement(6603..8021)
                     /locus_tag="VI935_03690"
     CDS             complement(6603..8021)
                     /locus_tag="VI935_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HLE24736.1"
                     /translation="MTSDNSDNLENHTFEEIQIGNSASLTRTLTKKDIQLFAVMSGDV
                     NPTHLDEEFTNKFLAGGLAGHSMWSGSIVSAILGTELPGPGTIYLSQDLRFLRTINLN
                     DTVTVNVTVREKRPNKKSVIFDCSCINQRGETVMTGTAEVIAPSEKIVIPRPLRPEIS
                     LQMHDKYARLIERCSKFPPIVTAVAHPCDESSLTAVLDAVKTGIIEAILVGPAQKIKA
                     VAEGLGFDISPYLIVDAPHSHGAAAKAVELIREGKAEVLMKGSLHTDELMREVVRSDT
                     GLRTKRRITHAFIMDVPTYHKPLIITDAAVNIYPELDEKVSICQNAIDLAHVLGIECP
                     KVAILSAVETVNPKIKSTIEAAALCKMAERGQITGALLDGPLAFDNAISKMAAQIKKI
                     ESSVAGDADIILVPDLESGNILAKQLTFMANADAAGIVLGARVPIVLTSRADSGRARI
                     ASCAIAALIADARHRANLKSEV"
     gene            complement(8046..10217)
                     /locus_tag="VI935_03695"
     CDS             complement(8046..10217)
                     /locus_tag="VI935_03695"
                     /inference="COORDINATES: protein motif:HMM:NF022783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HLE24737.1"
                     /translation="MEDKKPVNPFSFYSDFMKSTWDYLIDSYQRSVLFWDVMRKRGNT
                     FLEHYEKGKPPVLRFKYEILLDGRDLERPCNYVLLRIPPEKGVKIDPKKRPFVVVDPR
                     AGHGPGIGGFKYDSQVGVAMRAGHPVYFISFFPDPVPGQTLGDIGYAEVLFIEEVAKR
                     HPNSEGKPCVVGNCQAGWAVAALAALRPDVVGLIILNGAPLSYWAGEDNKNPMRYLGG
                     FLGGSWMTSLACDLGNGLFDGAHIVTNFERLNPANTYWSKQYNLYANIDTEEPRYLDF
                     EKWWGGYFLMTEDEIEHIVNDLFIGNKLGKGAIESKSGAHVDLKNIQSPVVIFASHAD
                     DITPPQQALNWIVDVYGHEDAIVENGHVIVYLLHEDIGHLGIFVSGRVAKKEHTELVN
                     VMEMIDRLPPGLYEMVIERKPDAHRYGELEEGEYTVRFETRKVKDILALDESREDEAL
                     FSTIDQVSKINNLLYKTFISPYVKAMSTETSATFLKLLHPLRMQHYLFSDLNPFMASI
                     ATMANWVRSKRKPMPNDNRFLALEKEISQCIEETLNAYRDIRDNSTRNFVSALYGPFG
                     LGAIFPPEQPEKIETKEGIKTQLEAKKAQMENRFEEGGFVEAVVRMLIAGIKKKGAAE
                     RRSFLIAEKLRKQNGDLRWPTRSEFKKLVREQTLILSMDPERAIQALPKLLPTKEERI
                     KAVALVGKILMVEPELADPKSQLAKRVKEILGVELTFPKGA"
     gene            complement(10238..11080)
                     /locus_tag="VI935_03700"
     CDS             complement(10238..11080)
                     /locus_tag="VI935_03700"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020585308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HLE24738.1"
                     /translation="MKKVGVVGAGTMGSGITQVVASNGREVVLLDISDSAVERGMKAI
                     GNSLGRFVKKGEISEEDSNSILSRIKTTTKFEDLRDVDLVIEAIFEDMNVKKETFKKL
                     DASTRPDVILATNTSSLPIIEIAVNTKRPDKVVGMHFFNPVPIMKLVEIIKTIATSDE
                     SVEFAYDFAKALGKEPVKTKDVPGFIVNRVLMPMLNEAVFALEEGVGTAEDIDKAMKL
                     GTNQPIGPLALIDLVGLDVTLNVIDVLYREFQDPKFRAAPLLRQMVRAGWLGRKTGRG
                     FYKY"
     gene            complement(11304..12077)
                     /gene="scpB"
                     /locus_tag="VI935_03705"
     CDS             complement(11304..12077)
                     /gene="scpB"
                     /locus_tag="VI935_03705"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HLE24739.1"
                     /translation="MFILSELKESIGTITLNNPAKRNTLVRELIEELITTLENFRKAS
                     ARVVVLRAPGDSKVWSSGHDVSELLQDPDPLSFSDPLGRALRAVRIYPAPVIAMVHGS
                     VWGGATDLVLSCDIAIGDETSSFAITPVNLGLPYNTTGLTRFMKRLPLNFVKEMFFTA
                     APIKAADAEKWGILNHLVPASELEKFTYDMAHLIASKAPLAVSVVKEQLNILADADPI
                     TPAIFERIQELRQRVCESVDYGEGIRAFYEKRKPIFTGK"
     gene            complement(12099..12500)
                     /gene="atpC"
                     /locus_tag="VI935_03710"
     CDS             complement(12099..12500)
                     /gene="atpC"
                     /locus_tag="VI935_03710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HLE24740.1"
                     /translation="MADKILLEVVTPSMLAVKEEVDEVVAPGELGEFGVLPGHVLFIT
                     LLMPGELRYTKGGVERRYIISGGVAEVREDKVSILTDNVEDPAGIHTEAARRELEAIL
                     EELKNFSGRKKELEELYRRLKIAQVRANVKE"
     gene            complement(12586..14004)
                     /gene="atpD"
                     /locus_tag="VI935_03715"
     CDS             complement(12586..14004)
                     /gene="atpD"
                     /locus_tag="VI935_03715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HLE24741.1"
                     /translation="MGTDVGNGKVVQVMGPVVDLEFKEGELPPIFTAIRITNPLINDK
                     KWNLVLEVAQQLGGRRVRCIAMDSTDGIKRGEDALDTGEGISIPVGKEALGRLMNVVG
                     EPIDEFGPIEAKERWPIHRPAPQFVEQSTKLEVFETGIKVVDLLAPFLKGGKIGLFGG
                     AGVGKTVLLMELIHNVAKKHGGVSVFGGVGERTREGNDLWLEMRESGVIKNTGMVFGQ
                     MNEPPGARARVGLSALTLAEYFRDTEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAV
                     GYQPTLATDLGELQERITSTVKGSITSVQAIYVPADDLTDPAPATTFAHLDGTIVLSR
                     QLTELGIYPAVDPLDSTSRILDPRIVGEEHYKIAREIQRVLQRYKQLQEIIAILGMDE
                     LSEEDKLTVARARKIQRLLSQPFSVAEQFTGTPGKYVPIKETIVAFKEILSGNMDEVP
                     EQAFYMVGNIEEVYVKARQVIS"
     gene            complement(14006..14887)
                     /gene="atpG"
                     /locus_tag="VI935_03720"
     CDS             complement(14006..14887)
                     /gene="atpG"
                     /locus_tag="VI935_03720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HLE24742.1"
                     /translation="MPSLRQIRRKIGSVKGTQRIMKAMKLISAVKLQRAQGLLQAFRP
                     YSNNFEEVARSLATRCDPKWHPLLRRPEERKKLHLVFMTSDRGLCGSFNSNLIRKIQT
                     YLVVDAKAYEQVTFTFVGRRGRDYFARGKTLATKSYIGVNERNFSEVSGEIAFALIED
                     FIKGKCDEVILIYNFFRSALSQIMTFEKLLPIEPKDIFDDSQTVDYLYEPGRVEVLKF
                     VLPKYIEVRVERAILESITSEHAARMAAMENATRNADDVIRKLSLLFNKTRQSLITKE
                     LMDIVNGTEALRKGGLE"
     gene            complement(15018..16535)
                     /gene="atpA"
                     /locus_tag="VI935_03725"
     CDS             complement(15018..16535)
                     /gene="atpA"
                     /locus_tag="VI935_03725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HLE24743.1"
                     /translation="MEEIKIEEIGEILKGRIKGYEREIVTSEVGTVTSVGDGIARAYG
                     LSEAMAGELVEFKGGIMGLVLNLEEDNVGIAIFGEDKHVEEGDMVKRTGRIAEVPVGD
                     DMKGRVVNALGQPIDGKGQIEAKEYRQIEIKAPGVVHRQPVREPLQTGIKAIDAMIPI
                     GRGQRELILGDRQTGKTAIVVDTIINQRGQDIYCIYVAIGQKQSTVAQVVDKLREFGA
                     MEYTTIVAATASEPAPFQFIAPYAGCTLGEYFRDTGRHALVIYDDLTKHAWAYRQLSL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKMRDEDKAGSLTALPIIETQAGDVSAYIPTN
                     VISITDGQIYLESDLFYAGVRPAINVGLSVSRVGGSAQIRVMKRVAGRLRLELAQYRE
                     VEAFSQFATDLDKVTQAQLARGSRLVEAMKQDQYQPLPVEKQVLLIYAVTNGYVDGYP
                     VEAVKKYEKELFVFFEAKYQNLLEEIGTKKDVTDALVQKVRKALDELRERLGELK"
     gene            complement(16649..17191)
                     /gene="atpH"
                     /locus_tag="VI935_03730"
     CDS             complement(16649..17191)
                     /gene="atpH"
                     /locus_tag="VI935_03730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HLE24744.1"
                     /translation="MASIATLRDLTEALLQASKEEGKLDSVASDLEGFLKLLSSQDEV
                     RNILGSSVYDVNVRKEIVSGIGEKVGFDKLTINFLNLVIELGKFKALVKSEAPFMQKI
                     RKASGRVKAEVTVAVNPSQENLDRIKERLNNLMEKEVEITVKVDPSILGGVIAKVEDK
                     VFDGSIKTQLERIRRILSVA"
     gene            complement(17170..17727)
                     /locus_tag="VI935_03735"
     CDS             complement(17170..17727)
                     /locus_tag="VI935_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HLE24745.1"
                     /translation="MIISDLVSLVITTAAQEGGGFNWSYVAQYTVNLVILLAVLVYYL
                     KKPIRNFLIERRGIIGYSIDEAERVIAGARKRYEEYAEKMSKIDVEIKALRKILKKEG
                     EIERNEILRHAELASQKIREEARETTRLETAKAKQEIQSEAVSLAIGLAEGILKQNLI
                     ESDEGRFIDDFVKRVDEEKWRQSQH"
     gene            complement(17730..18242)
                     /locus_tag="VI935_03740"
     CDS             complement(17730..18242)
                     /locus_tag="VI935_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HLE24746.1"
                     /translation="MKKLILLKELFFVFTILIISTIDASAAEDLLKVDKTVIIQIVIF
                     LAAIFILNPLLFKPLLQLVERREQLTTGRIKEAKELEKKVEQIIKEYKEKLDEVRAKA
                     MEERNEIRRQAEAAAEEVIAKAHNEVQALLEDARAKLELKAKEIRAKIKPEIEMLARN
                     VASQVLGREL"
     gene            complement(18555..20297)
                     /gene="recJ"
                     /locus_tag="VI935_03745"
     CDS             complement(18555..20297)
                     /gene="recJ"
                     /locus_tag="VI935_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HLE24747.1"
                     /translation="MKKHWVIEKHNPKLKEKLVKALGISSITAQVLINRGIKDEAEAE
                     LFLKSTLFDLPSPYLMRGMDKAVERIERAIQGKEKIAIYGDYDVDGVTATSLFYTLLK
                     NLGVNVTYYNRDRLKEGYGVNLQAIKKLKNDGVSLIISGDCGITAWKEVQDAKKLDVD
                     FIVTDHHEPPETIPEAVSVLNPHQIGCSYPGKEIAGVGVIYNLAIALRRALRESGFFR
                     KARPEPASSSGETAHDRNEPNLGDFLDLVALGTVADCASLTNVNRILVKEGMKRMLSP
                     KRPGMIALKEVSGIEGEVNSYDIGFKLGPRINATGRLQSAGVAVELLISQDLKQAREL
                     AKILNQENSKRQNIEEDIFLDTVSQIESNPEFLKSNSLVLSSPNWHAGVIGIVATKLV
                     ERYQKPAILISIQESGVGKGSGRSIEGINIYAALSECKELFEQFGGHQLAAGISIKEE
                     RIDEFRKRFERAISESAGDFVSMIKIDDVVDLNEITDEVVSELELLAPFGIGNPEPVL
                     LSKSLEVISERLFKEKHLSLKLKQKDRVFDAVWFNLKEPIKVPNRIDMAFTPEFNVWN
                     GKKEVRLRIKDIDY"
     gene            complement(20517..20960)
                     /gene="aroQ"
                     /locus_tag="VI935_03750"
     CDS             complement(20517..20960)
                     /gene="aroQ"
                     /locus_tag="VI935_03750"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015201276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HLE24748.1"
                     /translation="MKILVIHGPNLNLLGKREPEIYGKTTLEEINSLIRKEAKALRVQ
                     VESFQSNSEGEIVTKIQDATKKFDGIIINPAAYTHTSVAIRDAISSTGIPTVEVHISN
                     VYRREEFRQKSFISPVASGVISGFGVYSYILGLRGLVSQLKKSNH"
     gene            complement(21479..22195)
                     /locus_tag="VI935_03755"
     CDS             complement(21479..22195)
                     /locus_tag="VI935_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24749.1"
                     /translation="MGVLNITTLFSDFVDNVADNQGKPRIKKTQGGITLNVAIPNMPL
                     LSIYYSHGYSRSVYEPNGYQPQKNWLDNFGATLYYTRPNWDILVSSTSTISSDKFFSD
                     SNSIAFFYQLSGSYRPVSSITISPTLSLGDERYVWSGGGARYITPTASIWFGYYPLLD
                     DFNLSLYGSYSINKSNDGYTDTNALNGTIGLFWTLAKSSFGKHSLSFDVSYYHYLNDV
                     YANSSYKDISALLNLKLASF"
     gene            complement(22247..22402)
                     /locus_tag="VI935_03760"
     CDS             complement(22247..22402)
                     /locus_tag="VI935_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24750.1"
                     /translation="MIYSPYQDFDYREGFIEYQPLLIRFGSKGSLGGFDYGVHYLDVR
                     NGVGKVI"
     gene            complement(22712..23167)
                     /locus_tag="VI935_03765"
     CDS             complement(22712..23167)
                     /locus_tag="VI935_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24751.1"
                     /translation="MPSRIIWISVVLLFLWFSPKYGNTEPQLYSVRVGNHGKFSRVVF
                     EFDKDTPYEIIPEKDPFKIVVEFGKLHTTQSHVVFWVHDPVVQEVSIIDTPTKIKAEI
                     TLKKGGIVKKHFCLSAPPRIVFDIIHDTSISFDNSIETPQIQESPTNER"
     gene            complement(23570..23749)
                     /locus_tag="VI935_03770"
     CDS             complement(23570..23749)
                     /locus_tag="VI935_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017662846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HLE24752.1"
                     /translation="MRFLDGLTNRMGIAGELLQFFLQHKWWWLTPMILVLLIFGLLIV
                     FAQSSAIAPFIYTLF"
     gene            complement(24013..24195)
                     /locus_tag="VI935_03775"
     CDS             complement(24013..24195)
                     /locus_tag="VI935_03775"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HLE24753.1"
                     /translation="MYKFQRLEVYKLALEYVNAVYDLSRRLPESERFNLRSQVERAAT
                     SIVLNIAEGSTGQTDK"
     gene            complement(24238..>25986)
                     /locus_tag="VI935_03780"
     CDS             complement(24238..>25986)
                     /locus_tag="VI935_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017662844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HLE24754.1"
                     /translation="DAAAALIRDGQLIAAAEEERFTRKKHDYEFPQNAIDFCLRIGGI
                     KGPELDYVVFFEKPFVKFERLLLSTMQTFPHSLRLFREAMITWFGDKLWIKHLLQKRI
                     GVPASKILFSEHHLSHAASAFFCSPFDEAAILTVDGVGEWTTATLGIGKGTNIKLLQE
                     IRFPHSLGLLYSAFTAFLGFEVNEGEYKVMGMAPFGTPRYMDKVYKLIQVSSDGSFEL
                     NMDYFSFHYSSEKTFNRKFEGLFGSPRDPKSHFFTKESGYPSYFGEKPSNYEELAQEN
                     KYYADVAASIQAVTEEVLLKMAHSAYKETGLKRLCMAGGVALNSVANGRILKETPFEE
                     IYIQPSAGDGGGAIGAALFGFHMVLGKPRNFIMEHAYWGEEHSPCGIEKFLTDNGIRY
                     ERIEDEQKLINRVVDRLQEGKVIGWHQGRFEWGPRALGNRSILADPRRVDMKDIVNVK
                     IKFREPFRPFAPSVLVERTQDYFSLDHSARHYPARFMLYVVDVRKEKQDVVPAITHVD
                     GTGRLQTVQKEVNPRYYNLIETFEQATGVPVVLNTSFNLKGEPIVNTPKESFLTFSRS
                     GMDVLVLGDYIIEKTG"
BASE COUNT         7008 a         6075 c         5234 g         7669 t
ORIGIN      
        1 ggtttaatat agaagggtat taatgcaggt caataaattt ttaaggaggg agacgtggat
       61 gaaaaagtta attctaagct taattattac ctttttgatt attttccctc caactttacg
      121 tgctcagaac tttagttctg gtggatttag caaaggtggg tttagaggcg gtggttttac
      181 tcgcaccgga ggtgttagta aaatctcggg ttttagcttt agaggcgggt tcagtaatag
      241 cgcagcgacc tcaaacactt ccggaatggg atcgaaaaat ggaaatagca gaggctttac
      301 aagatcaaat tttaataggg gaggcttaag tcccaatctt tccaataaac ctaacgctag
      361 attgtttaaa aatactgcaa atatacaaag accttctata agcaccggca gctcaaataa
      421 caatcttcca actcttcata ttccagaagg agctatagtc aaatcagtta aaagtgaacc
      481 taagaacatt agtccaaatt tacatacaaa gagtggtgac tttagatcaa attcagtaaa
      541 aaacgggatt atagctaaag aagacagaaa tatagctcta agagataatc ttaattcaac
      601 aactaaggga agaactaatc ctttcacaaa cggccgtact aggtcacaat taatgtcaag
      661 aggattaaac tcaaatactt tgaattataa tcgcatttct aatataaaga atgtcaacgt
      721 tagcccaaac tcaataatta acagagtccc accgagaaaa ggaatcagtc caatccaaaa
      781 tgatctttca aatactcctt ccagaaatgc tgagatagct cattgggttg acaatggtgt
      841 tatgcatttt actaaccgtg tcagttcact tccaaatgga gtaaagacag ttaatgttaa
      901 taacggaaat aactcggttc cagtatcaag agatttaaac gcagatagga atctttctac
      961 ctcaggtcct ttgataaatt ccttgtctac aaactcaaaa aacccagtaa ggctttctat
     1021 gaaacagaat actaattcta gacattctaa aaacggtttt gttaatgata atattcacgt
     1081 gtcaactaat aacgtattta atttttcatt taacttcttt tttcctagtc cattcttttt
     1141 tcaacccttt ttcttccccg cgctctttga tccatttgta tttaatcctt tatttccatc
     1201 accttttttc acatttaatc ccttaatacc tccacctgtg atcgatccat ttctattcca
     1261 accatttttc gtatcgccgt tctttaatcc gtttcccttt agcacggtct ttttcatcaa
     1321 tacgtctgta tctaatgatg taataattga ctgatcgagt gatgtaaaat ccatccaatc
     1381 tgttggagca cagacaagtc tgtgcctaca aactacttgt ggtttccttg acaatcaaag
     1441 gggatttggt acaatccttc actgatttta agcgtgggta gggattatat gaagacatat
     1501 atggcaagga gtgaagatat acaaaagaaa tggtatctca tagatgcaaa cggtaaaaca
     1561 gttggaaggc ttgcaacaaa aatcgcaact atcttaagag gtaaaggaaa acccgagttc
     1621 accccacatg cagatatagg agattttgtt atcgtaatta acgctgatag ggttcatttt
     1681 acgggaaaaa aatggagtaa aaaagaatat tattggcata caccctatcc aggtggttta
     1741 aagtctataa cagcagagaa attgtttaaa gaaaagcctg aggaaatctt acaaaaggcc
     1801 gtttggggaa tgcttcccaa gacaaaatgg caaaaaaaat taattaccag gttaaaaatc
     1861 tattcaggga atgaacaccc tcatatagtc caaaaccctg aaaagttgga ggcgtagtta
     1921 gttctaatgg ccgagattac gtatcatgga actggaagaa gaaaaacctc agttgcgcgt
     1981 gtcctgttac gaacgggtga tggaaagatc tcaataaatg atagaccagt tgagttttac
     2041 tttccacgtg aagcatggag gatacacgca cttgagccgc tccaggttac tcaaacatca
     2101 ggaaaattcg atgtgatagt agatgtaagt ggaggcggtc tcaggggtca gtcaggcgct
     2161 ataaggcacg ggcttgcaag ggctcttctt catgttaatg aagcatttag aaaggcactt
     2221 aaaagcgcag ggcttcttac tagagacccc agaatggttg agagtaagaa atacggaaga
     2281 agaaaggcta gacggggtca gcagtactca aagaggtagt attctttggg acttggggta
     2341 tccccgatcc ccgcggtcca ttcggggcgt ggatcaaaga gggagaattt tagggaacca
     2401 tgggatctgg ggtagcgatc aaagagggag gatttaaggg aacctcttgc caaccaaaaa
     2461 aaagaaatcc aataacaggt tcgaccgttc gacaggctca ctgctcacta caggtaacaa
     2521 gagaatcgag attataccgc ctcaaggtag gcttggcgtt ttactgcctg gactaggtgc
     2581 tgtaagcaca acattcattg ctggtattga agcaattaaa agagggcttg gaaaaccttt
     2641 cggttctctt acgcagatgg ggacaataag gcttggaaaa agaacagaaa aaaggtcccc
     2701 tcttataaag gattttgttc cacttgtaaa tcctgaagat tttgtatttg ccagttggga
     2761 tattttcccc gataactgct acgaagctgc tcttaaatcc ggtgtgctcg aaaagaacct
     2821 ccttgaggaa ttaaggtttg aacttgaaga aattgagccc atgcctgctg tttttgacag
     2881 aagatatgta aggaacctta agggtattca cttaaaaaaa gaaaagacca aaatgcagct
     2941 tgcagagcgg cttattgatg atattacaaa ctttaaaaag gaaaataagc ttgaccgtct
     3001 tacaatggtc tggtgtggca gtacagagat ttatataaag ccttcaaagg ttcatagcac
     3061 tattcagaat tttgaggagg gtctaaagga caaccatact gaaattgcgc caagtatgat
     3121 ttatgcatat gctgcgattc aatgtggagt accatatgca aatggagccc ctaatctatc
     3181 agccgacata cctgccctta ttaatctggc taagaggaaa aaggttcctc tagccggtaa
     3241 ggattttaaa acaggacaga ctctaatgaa aacaattatc gctccagggc taaaggccag
     3301 gatgctaggt cttagcggat ggttttctac taatatctta ggaaacagag atggcgaggt
     3361 cttagacgac ccatattcat ttaagacaaa agaggaaagc aagctaagtg tgcttgaata
     3421 tattcttcaa cctgacctgt atccggagct ttattcagat tattatcata aggtaagaat
     3481 taactattat cctcctaggg gagacgcaaa agaggcatgg gataatatag acatattcgg
     3541 atggcttggt tatccaatgc agataaagat taatttcctc tgccgagata gcattcttgc
     3601 cgccccaatt gttcttgatc tggttctatt tcttgacctt gctcgaagag caggtttata
     3661 cgggattcag gaatggctct cattctattt caagagcccg atgtttggtg aagggcttta
     3721 tccagagcat gacctcttta ttcagcttat gaaactaaaa aacaccctca gatacctaaa
     3781 aggggaagag cttattaccc accttggatt ggattattac gactactacg agcctgggtg
     3841 agttgtagaa ataaaaatcg catttaacaa tttggaaact tttatttcat gcgagggatg
     3901 aatcccaaag cgataaaaaa attgattggt tgactgggat cgtaatgatt ttgtttgtgg
     3961 attactccat taagattaat cgattctctc ctttgatgaa tccttccaat ggtctttgtg
     4021 tgagccaatt tttttaagct tctcctccat ttccttgagg acttcgattt gatgtttatc
     4081 aaccggacgg tcgaatcctt cactatgcca aatctgtata cccaaagcat aatttcttct
     4141 atcggtatct ttatctccag gtatgtagaa ggaacgggaa tcgtgaatct taatctcaaa
     4201 gcggacgttt ggattatctt taggaatcag ccaatcggca attcgtttga gtttctggaa
     4261 tacgttaggt atttctgtat catgaggagt ttcttcatat gttccagaaa ttactaaagc
     4321 taaagcaacc tgtactcttc gtccactctt ctgatctgga agctctattg gtcctgttat
     4381 ccaaaatacc ttgtgttcct ctatgatccg acttagctgt gaaatttctt catccgcaga
     4441 aattattttg atatccttta actccgaatc gttttttgac attgtttata atcccttatt
     4501 tagttttttt attttgtttc gccaaattca aacctccaga taatcaacta cttcccagtc
     4561 atttccttga tctatgtcat gataaaaatc tagtcaatta tatgatagcc gccgtcaatg
     4621 taaatggtct cgcctgtaat cagcttcgca gcgtcggtcg ccaagcccgc agtcgcagct
     4681 ccaacatcgt cgatagtaac tagacgcttg gctggagcct tagcggccgc aatgttcagg
     4741 agatcatcga agtccgcaag accagaagcc gctcttgttt ttattgggcc aggggaaatg
     4801 gcatggaccc tgatctttgg acccagctct gctgctaggt atcgaactga agcttcaaga
     4861 gccgccttga ttggtcccat aaggttataa tgctgcacaa ccttttgcgc accgtaatag
     4921 ctcatggtaa aaagtgttcc gccatccttc atcagaggct ctgccaatct tgccatacgg
     4981 atgaaagaat gacacgagac atccatagcc aaaaggaaac cttcacggga cgagtcaacg
     5041 agccgccctt gcaaatcctt ctttggagca aacgcaatgg aatggagtgc gatgtcaagt
     5101 ttaccccatg tccgttcaat ctctgagaag actgcctcga gttccccttc ctttgtcacg
     5161 tcgcaagaca tgaaaatagg tgctccaagt tccttggcaa ggggttcaac gtatggttta
     5221 gacttttcat ttccataagt caccgcgacc gcagccccac ggaatttgaa agcctttgca
     5281 caaccataag caattgaatt ttcatttgct ataccaatca caagcgcatt cttgcccttg
     5341 agcattattt ggtctgagtc gaacatgagc agcctcctta tctccttttg agattattgc
     5401 gtcttatcaa gaccaagagc ctccatggta tgacgcgcga ttacatattc ctcgttggtt
     5461 ggaactaccc agacggatac ggggctatca ggcttagtta tgcagagttt gttggatttg
     5521 tttgcaactt catcaatgtg aattcccaac caggctgagc gggcacagat acgagaacgg
     5581 atttcagaac tgttttcacc gataccggct gtaaaaacaa gtgcatcaag cccgcccatc
     5641 gccgatacga gagacccaag ttcacaggca acacggtaaa tgaaatagtc aaccgcttcg
     5701 gcagcgcgtg ggtcagtgct ggataaaagg cggcgcatat cattgctcac gccagataat
     5761 ccaagcaaac cggagcgctt gtagagcaaa tcttctacct tatcaatact gaatccccgt
     5821 tcacggacga ggtagattat tacacctggg tctaaattcc cacagcgggt tcccatcgga
     5881 agccccccta ccgcggtaaa ccccatagtg gttgcgacac ttcgaccatc cttcatagca
     5941 cacatgcttg cgccacttcc aagatgagct acaacagctc gacctgaagc tattcgggga
     6001 gatatggttt taagtctttt agatatatac tcgtatgaga gaccgtgaaa gccgtaacga
     6061 cgtattccct tatcataata ctcatatgga agcccgaaaa gctccgccac cttaggctga
     6121 gtgcggtgaa aagaggtgtc aaaacaggcg acttgtggaa gcctgggatt ataacgtgtt
     6181 acggcgcgga ttgcggcaag gttatgcggt tgatggagag gcgcgagggg aacaaaggat
     6241 tccagcttgg ccataacttc gtcattaacg acagccggag aggcgaaatc aggcccgccg
     6301 tgcaccacac ggtgaccaac ggctgccagt ggatatccgg gacgattggt tctcagccag
     6361 ccagcaagcc tttccaaaga atccgcctga tttgatactt ccgccgcaga ccagtgttct
     6421 tctgctattg tcgcgccatc actgtccttc acaataaaac gaggttctgt tcctattccc
     6481 tcaatctgtc cctttaggaa tacaccgagg tccgccccac tatccaggga aaacacagaa
     6541 aatttaaggc ttgatgatcc ggcattgaga attaagatta gattcttcat gtgagcaccc
     6601 cgtcacacct cagactttag gttagccctg tgtcgcgcat ccgcaatgag ggccgctatg
     6661 gcacaggatg ctatgcgagc acgcccgcta tcggcacggc tggtcagtac gatgggaact
     6721 cgcgccccaa gcactattcc cgcggcgtct gcgtttgcca tgaaggtgag ttgctttgca
     6781 aggatattgc ctgactccaa atctggaaca agaataatat cggcatctcc agctactgac
     6841 gattcgatct tctttatttg ggctgccatc ttgctgatag cgttgtcaaa ggctaacggg
     6901 ccatccagca aggcgcccgt aatttggccg cgttctgcca tcttacagag agcggcagcc
     6961 tcgatagtag atttgatttt gggattcacc gtttctacag cggagaggat agcaaccttt
     7021 ggacactcga ttccaagaac atgggcaagg tctatagcat tttggcagat agaaaccttt
     7081 tcatctaact ccggatagat gttaactgcg gcatctgtga tgatcaatgg cttgtggtag
     7141 gttgggacat ccatgatgaa ggcgtgagtg attctccgct tggtcctcaa tcctgtatca
     7201 ctccgcacga cctcgcgcat gagttcatcg gtatgcaaac tgcctttcat taagacttcg
     7261 gctttgccct ctcgtatcag ctcaacggcc ttggccgccg ccccatgact atgaggggca
     7321 tccacaatga ggtatgggga tatatcaaaa cccagacctt ctgcaacagc tttgattttt
     7381 tgcgcagggc ccacgagtat cgcttcaatt atgccggtct ttaccgcgtc taacactgct
     7441 gttaatgatg actcatcgca gggatgggca accgcggtaa ctatgggcgg aaacttgcta
     7501 caacgctcaa tcaatcgggc gtatttgtcg tgcatttgta aagagatctc gggtctcagg
     7561 ggtcggggta ttactatctt ttcagaagga gcgataactt cggcagttcc ggtcataacg
     7621 gtctcaccgc gctggttgat gcaggagcaa tcaaatatca cgctcttttt gttagggcgc
     7681 ttttcacgaa ctgttacgtt gacagtgaca gtatcgttca aattaattgt cctcagaaaa
     7741 cgcaaatctt gtgatagata aatggttccc ggtccgggca actctgtccc gaggatcgcc
     7801 gacactatag aaccactcca catactgtga ccggctaaac caccagccaa gaatttattg
     7861 gtaaattcct cgtcaagatg ggtagggttc acgtcccctg acatcactgc aaaaagctga
     7921 atatcttttt tagtcagggt gcgggtcagg cttgccgagt tacctatctg gatttcttca
     7981 aaggtgtgat tttctaaatt atcactatta tcacttgtca tagaacccct ccttatcaat
     8041 tgactctaag cccctttggg aaaagtaagc tccacgccga gaatttcctt gactctcttg
     8101 gcaagctgtg atttaggatc agccaactca ggctctacca ttagaatctt gcctaccagg
     8161 gcaaccgcct tgatacgctc ctcctttgtc ggcaggagct tgggaagagc ctgtattgcc
     8221 ctttccggat ccatactcaa tatcagggtc tgttcccgga ctaacttctt aaattcggag
     8281 cgggtgggcc aacgtaggtc gccgttctgt ttacgtaatt tctccgcgat aaggaatgaa
     8341 cggcgctctg ccgctccctt cttcttgatt ccagcaatta gcattcgcac cacggcttct
     8401 acaaatccac cctcctcgaa tctgttttcc atttgtgctt ttttcgcctc taactgtgtt
     8461 tttatacctt ccttagtctc aattttctca ggttgttccg gaggaaatat cgctcccaga
     8521 ccaaacggac catataatgc actgacaaaa ttacgagtag aattatcgcg aatatcccta
     8581 taggcgttca aagtttcttc tatacattga gaaatttctt tctcgagggc aagaaagcga
     8641 ttatcgttgg gcattggctt tcttttgctt ctgacccagt ttgccatagt ggcaatggaa
     8701 gccataaagg gatttagatc tgagaaaagg tagtgctgca ttcggagcgg gtgtagaagt
     8761 ttcaaaaaag tggccgatgt ttctgttgac atggctttaa cgtaggggct aatgaatgtc
     8821 ttataaagaa ggttattaat tttagagacc tggtctatgg tactgaacag cgcttcgtcc
     8881 tcacggcttt cgtccaaggc aaggatatct ttcaccttgc gagtctcgaa gcgtaccgtg
     8941 tactctccct cttcaagctc tccatacctg tgggcatctg gcttgcgctc aatcaccatt
     9001 tcgtacagac ccggcgggag tctgtcaatc atttccataa cattaacaag ttcagtatgc
     9061 tctttctttg caaccctacc tgagacaaaa attccaagat gtcctatgtc ttcgtgcaac
     9121 aaatagacaa taacatgtcc attttctact atcgcatcct cgtggccgta aacgtcaact
     9181 atccagttta gcgcctgctg gggaggggtt atatcgtctg cgtgtgaagc aaatatcacc
     9241 acaggcgatt gaatattttt gagatcaaca tgagccccac ttttagattc tatagcacct
     9301 ttccctaatt tattgccgat aaaaaggtca ttgactatgt gctcaatttc atcctccgtc
     9361 attaggaagt acccacccca ccatttctca aaatcgagat acctcggttc ctcggtgtca
     9421 atgttggcat agaggttgta ctgcttgctc caataagtat ttgcaggatt caatctttca
     9481 aagttcgtaa cgatgtgcgc gccgtcaaaa aggccattgc cgagatcgca tgccagtgag
     9541 gtcatccagg aaccgcccag aaacccgccc aaatagcgca ttggattctt gttgtcctcc
     9601 ccagcccagt aagacagggg agcaccgttg agaataataa gccctacaac gtctggacgc
     9661 aaagcagcca aggctgcaac cgcccatccg gcctgacaat tccccaccac gcatggcttc
     9721 ccttccgaat ttggatggcg ttttgcaacc tcttcaatga ataatacttc ggcatagccg
     9781 atgtccccaa gggtctgacc gggaacagga tccgggaaaa aggatataaa ataaacagga
     9841 tgtccagcac gcatcgcaac cccaacctgt gaatcgtatt taaaaccacc tattccaggc
     9901 ccatgtccgg cccgcggatc aacaaccaca aaggggcgtt tttttgggtc aatctttaca
     9961 cctttctcag gcgggatccg taacaggacg tagttgcagg gacgctccaa gtcacgccca
    10021 tcaaggagaa tctcgtattt aaagcgcaga acagggggct tgcctttttc gtagtgctca
    10081 agaaaggtgt ttcctctctt gcgcatgaca tcccaaaaca aaaccgagcg ctgataagag
    10141 tctataagat aatcccaagt gctcttcata aaatcagagt aaaatgaaaa aggattgaca
    10201 ggttttttat cttccatttt aaatacctcc atttgtatta gtatttataa aatccccttc
    10261 ccgtttttct tcccaaccat ccagcgcgaa ccatctgacg gagaagcgga gcagcacgga
    10321 attttgggtc ctgaaactcg cgatagagaa catctattac attgagagtt acatccaatc
    10381 ctacgaggtc tataagtgca agagggccga ttggctgatt cgttccaagt ttcatcgctt
    10441 tatcaatatc ttcagcagtt cccacaccct cttcaagagc aaacacggct tcattgagca
    10501 tgggcatgag aacccggttt acgataaatc cagggacatc cttggtttta accggctctt
    10561 taccaagggc cttggcaaag tcataagcaa actcgacgga ttcatccgat gttgcaatag
    10621 tttttattat ctctacaagc ttcataatag ggacaggatt aaagaagtgc atccctacga
    10681 ccttatcggg tctttttgta tttacagcga tttctataat aggaagcgaa gaggtatttg
    10741 tggcaagaat cacatcagga cgagtggatg catctaactt tttgaaagtc tcttttttca
    10801 cattcatatc ttcaaatata gcctctatga ccaaatcaac atcacgtaga tcctcaaatt
    10861 ttgtagtcgt cttaattctg gataaaatag aattgctgtc ttcctcgctt atttctccct
    10921 tctttacaaa ccttcccaag ctgttaccta tagctttcat tccacgctca acagcggaat
    10981 cagaaatatc caggagaaca acctctctgc catttgaagc cacaacctga gttattcctg
    11041 agcccattgt tcccgctcca acaacaccga ccttttttat atcttctaag cgcatagaaa
    11101 ccctatttac cctccttcca tcttttttgt gttagatttt catttagcta cttaagctga
    11161 gactttatct taatttattt gattataaac cacatatttt gcaagagtaa tgccagtaaa
    11221 aatttctttt attccgtata cattgaattt aaagaggtgt ctactgtctc aaattctgac
    11281 atacatgctc cctcaaaagg gcactattta ccagtaaata tcggttttcg cttttcgtag
    11341 aaagctctta taccttcccc atagtctacg ctctcacaga ctctctggcg cagctcttga
    11401 atacgctcga aaatagccgg tgttatggga tcagcgtcgg caagaatatt aagctgttct
    11461 ttaactacgg agactgctag tggggcctta ctggcaatca agtgcgccat atcgtaggtg
    11521 aatttttcta attcagatgc cggcaccaaa tgattaagaa ttccccactt ttccgcatct
    11581 gcagccttga taggcgctgc cgtgaagaac atttctttaa cgaaattgag cggaagccgc
    11641 ttcatgaacc gcgtcaggcc ggtggtgttg taaggcagac cgagattaac aggggtgatg
    11701 gcaaaagaac tggtttcgtc ccctatagct atatcgcaac tcagaaccag atccgtcgct
    11761 cccccccaca cagagccgtg aaccatagcg ataaccggtg cggggtaaat cctaacagca
    11821 cgaagagctc gtcctaaggg gtccgaaaac gagagtggat cagggtcttg aagaagctct
    11881 gagacgtcgt gaccagacga ccataccttg gaatcgccgg gcgcacgtag cacaacaacc
    11941 ctagctgatg ccttgcggaa gttctctaac gttgtgatta actcctcaat caattctcta
    12001 acgagcgtgt ttcgcttcgc tgggttattg agggtgatgg ttccaatcga ctcttttaat
    12061 tcagatagaa taaacatttg gtgagatttt gttggcagtt attcctttac gttcgcgcga
    12121 acctgagcga ttttaagtct tcggtatagc tcttcgagtt cttttttgcg tccgctaaag
    12181 tttttaagtt cttccaggat tgcttccaat tcccttcgtg cagcttccgt atgtataccc
    12241 gccggatcct ctacattatc tgtgagaata cttaccttat cttctctaac ctccgcaaca
    12301 cctccagata tgatatacct tctttcaaca ccgccctttg tgtatctgag ttccccaggc
    12361 ataagaagcg ttataaagag aacatgtcct gggagaaccc cgaattctcc aagctcccca
    12421 ggtgctacaa cctcatctac ctcttccttt acagctagca ttgaaggtgt tacgacttca
    12481 agcaaaattt tatccgccaa tttctatttc tccttgggcg tggttcgtga ttcgggtttc
    12541 gtggttcgat tcacgattca cgattcacga ttcacgaaat cgttatcatg atataacctg
    12601 cctggccttg acataaacct cttctatatt tccaaccatg taaaaggcct gctcagggac
    12661 ttcgtccata tttcctgaga ggatttcttt aaatgctaca atggtctctt taatcggtac
    12721 gtatttccca ggagtcccag tgaactgctc tgctacgctg aagggttgtg aaagaagcct
    12781 ttgaatcttt cttgcccttg caaccgtaag cttgtcttct tccgaaagtt catccatacc
    12841 aagaatcgct atgatttcct gaagctgctt gtaacgttga agcacccttt gaatctcacg
    12901 agcgatcttg tagtgctctt ccccgacgat tctggggtct aagattctag aggttgagtc
    12961 taatgggtca acagcggggt agattccgag ctctgtaagc tgacgggaaa gaactatagt
    13021 tccatctagg tgtgcaaatg ttgttgcagg cgccgggtct gttaggtcat ctgctggaac
    13081 gtagattgcc tgaactgaag tgattgaacc cttgacggtt gatgtgattc tctcctgaag
    13141 ctctccaagg tctgttgcaa gcgtaggctg ataacctacg gcagagggaa ttcttccaag
    13201 aagcgctgac acttcagaac ccgcctgagt aaatctaaat atgttgtcta taaatagaag
    13261 cacatcctgt ccctctgtgt ctctaaagta ttctgcgagt gttaaagcgg ataaccctac
    13321 tcgcgccctc gctcctgggg gttcgttcat ctgtccaaaa accatacctg tattcttgat
    13381 aacaccggac tctctcatct caagccagag gtcatttccc tcacgtgttc tttcaccaac
    13441 accgccaaag actgaaacgc caccgtgctt ctttgccacg ttatgaataa gctccataag
    13501 gagcacggtc tttccaaccc ctgccccacc aaagagaccg attttcccac ccttaaggaa
    13561 aggggcaaga aggtcaacca ctttaatccc agtttcaaat acctctagtt ttgtgctttg
    13621 ctctacaaac tgaggtgcag ggcggtgaat aggccagcgc tctttggctt ctatcggacc
    13681 gaactcatca attggttctc ccaccacgtt catcagtctt cctagagcct ctttcccaac
    13741 agggattgag attccttcac ctgtgtctag cgcatcctct cctcttttaa ttccatcggt
    13801 tgaatccata gcgatgcacc gaacgcgcct tccaccaagc tgctgtgcaa cttcgagcac
    13861 aagattccat tttttatcgt ttataagtgg gttcgtgatt ctaattgctg taaatattgg
    13921 tggtagttcc ccctctttaa actcaaggtc tacaacgggc cccatcactt gtacgacctt
    13981 tccatttccg acatctgttc ccattttatt ccagacctcc tttccttaaa gcctcagttc
    14041 catttactat atccataagc tcttttgtta taagagattg tcttgtctta ttgaataaaa
    14101 gagaaagctt tctgattaca tcatctgcat tccttgtagc gttttccata gctgccattc
    14161 gcgctgcgtg ttcgcttgtt attgattcta agattgctct ttctacgcga acctcaatgt
    14221 atttgggaag cacaaatttg agaacctcta ctctccctgg ctcgtaaagg taatccacag
    14281 tctgtgaatc atcgaatata tccttaggct ctattgggag aagtttttca aatgtcatta
    14341 tttgagaaag tgcagatcta aaaaaattgt agatcaaaat aacctcatca cacttacctt
    14401 ttatgaaatc ctcgatcaaa gcgaatgcga tctcacctga tacttcactg aaatttctct
    14461 cattaacgcc aatatagctt tttgtcgcca gggtcttacc ccttgcaaag taatcccttc
    14521 cccttcttcc aacaaacgtg aatgtcacct gctcgtaggc cttagcatcc accacgagat
    14581 aggtctggat ttttctaata agattgctgt taaagctacc acaaagaccc ctgtctgatg
    14641 tcataaatac cagatggagt ttctttcttt cctcagggcg tctcaaaagt ggatgccact
    14701 ttgggtcgca tctcgtcgcc agactccttg caacttcctc gaaattattt gaataggggc
    14761 gaaaggcctg aagaagaccc tgagcgcgct gaagctttac agcagaaata agcttcattg
    14821 ccttcattat tctctgcgtg cctttcacgc taccgatctt ccttctgatt tgtcttagac
    14881 taggcatggg ttctccattc gtgaatcgtg tatcgtgatt cgtgactcgt tatcttgtga
    14941 atcatgcatc gaaccagtag gggcaaccct tgcggttgcc caaaagaata gggcgggtgc
    15001 gagacccgcc cctacattta tttcaattct cccaatctct ccctaagctc atcaagagcc
    15061 tttcttacct tctggactaa agcatccgtg acatcctttt ttgttccgat ctcttcaaga
    15121 agattctggt actttgcctc aaagaaaaca aaaagctcct tttcatattt tttaactgcc
    15181 tccaccggat acccgtccac atacccattt gtaacagcat agataagaag cacctgcttc
    15241 tccactggaa gcggctgata ctggtcctgc ttcattgctt cgacaagtct gcttccacga
    15301 gcaagctgag cctgtgtgac tttatccaaa tccgttgcga actgtgagaa ggcctcaacc
    15361 tctctatact gagcaagctc aagccttagt cttccagcaa cacgcttcat cacacgaatc
    15421 tgagcagagc caccgactcg tgaaactgaa agtccaacgt taatagcagg ccttacgcct
    15481 gcatagaaaa ggtcactttc aagataaatc tgcccatctg tgatcgagat gacgtttgtc
    15541 ggaatgtatg cggaaacgtc gccagcctga gtttcgatta taggaagcgc tgtgagtgag
    15601 ccagctttat cttcatccct catctttgcc gcacgctcaa gaagccttga gtggagatag
    15661 aaaacatctc caggataagc ctcacgtccg ggtggacgcc gcagcaaaag cgaaagctga
    15721 cgatatgccc aagcgtgctt tgtaagatca tcgtaaatca cgagtgcatg tcttccggta
    15781 tcgcggaagt attcaccaag cgtgcatcca gcataaggag cgatgaattg aaatggtgca
    15841 ggctcgcttg cagtagcagc aacaatagtt gtatattcca tagctccaaa ctcccttaat
    15901 ttgtctacaa cctgagcaac ggttgactgt ttctgaccta ttgcgacgta gatacagtag
    15961 atgtcctgtc ccctctgatt gataatagta tcaaccacga ttgcggtttt tccagtttgg
    16021 cggtctccta gtattaactc tctctggccc cttcctatgg gaatcattgc atctatcgct
    16081 ttaatccctg tctggagcgg ctcacgaaca ggttgacgat ggacaacgcc cggggccttt
    16141 atctcaattt gtctgtattc ctttgcctca atctgcccct tgccgtcaat cggctgtcca
    16201 agcgcattta caacccttcc tttcatatca tcaccaactg ggacctcagc gattcgtcct
    16261 gtgcgcttta ccatatcgcc ttcctcgaca tgtttatctt ccccgaatat ggcgataccg
    16321 acgttgtctt cctcgaggtt taaaacaagc cccatgattc cacccttaaa ttcgacaagc
    16381 tctcccgcca tagcttcgct caggccgtat gcgcgcgcta tgccatcgcc tactgaggtt
    16441 actgttccta cttcagaagt tacaatttca cgctcgtatc ccttgattct accctttaga
    16501 atttcaccaa tttcttctat cttaatttct tccatcttgt tctatagctc cttcttaaaa
    16561 gttctgtagt cacttagcca cagaggacac ggagaaaatt taaaaaataa atcttttttc
    16621 ttttctcttc tctgtggctg aaaagttttc aggctacaga gagaatacgc cttattctct
    16681 ccagttgggt tttaatactt ccatcaaata ccttatcctc aacctttgct ataactccac
    16741 ctaggatcga aggatcaact ttaactgtta tttcaacttc tttttccata agattattca
    16801 gtctttcttt tattctatct aggttctcct gtgaaggatt tacagctact gtgacctctg
    16861 ccttcaccct tccggaggct tttctaatct tttgcataaa cggtgcttcg gattttacta
    16921 gagccttaaa tttgccaagc tcgatcacta agttaaggaa atttattgtg agtttatcaa
    16981 acccaacctt ttcaccaatc ccgctgacta tttcttttct cacattaaca tcataaacag
    17041 agcttcctag aatatttctt acttcatctt gagatgaaag aagtttgagg aacccttcaa
    17101 ggtccgatgc gacactatct agctttcctt cctcttttga agcctgaaga agtgcttctg
    17161 ttaaatccct cagtgttgcg attgacgcca tttctcttcg tctacccttt ttacaaagtc
    17221 gtcaataaaa cgcccttcat cagattcaat cagattttgt ttgagtattc cttcagccag
    17281 ccctattgcc aatgagaccg cttcagattg aatctcttgt tttgccttag cggtttcaag
    17341 tctggttgtt tcccttgcct cctccctaat cttttgagaa gcaagctcag cgtgtcttag
    17401 tatctcgttt ctttcgattt ctccctcttt tttaagaatt tttctgagag ccttgatttc
    17461 tacatcaatc ttgctcattt tctcagcgta ctcttcatac ctctttctcg cccctgctat
    17521 aaccctttca gcctcatcta ttgagtaacc tattatacct ctcctttcaa taagaaaatt
    17581 tcttataggt tttttaagat aatagacaag cacagcaagg agaataacta agtttactgt
    17641 atactgggca acataactcc agttaaatcc gcccccttct tgagcggcag ttgtaattac
    17701 taaacttaca aggtcagaga tgatcaaaac tataactccc ttccaagcac ttgtgaagcc
    17761 acgttacggg ctagcatttc tatctcgggc tttatttttg ccctaatctc cttcgctttc
    17821 aactccagtt ttgctctagc atcctctaag agggcctgaa cctcattatg cgctttggca
    17881 atcacttctt cagcggcagc ctcagcctga cgccttatct catttctttc ttccattgct
    17941 ttagccctta cttcatccag tttttcttta tattccttta ttatttgctc aacctttttt
    18001 tcaagctcct tggcttcttt gatccttcct gtcgtcagtt gctctcttct ttctacaagc
    18061 tgaaggagcg gtttaaagag aagagggttt agaatgaaga ttgcggctaa aaatattact
    18121 atctgaatga ttacagtctt atctactttg agaaggtctt cagcagcact tgcatcaatt
    18181 gtggagataa taaggattgt aaaaacaaaa aacaactcct ttagcaatat caattttttc
    18241 atgcgccgtg acctccagag ggttttacac ttgactccgg tagaacatat gtaaaggcac
    18301 agaaaatccc ttcaaaagga aattgacatg cttcttctat attcatctgg gattttacgt
    18361 tccttatgga gtataaaagc tataattttt taacataaaa ttaagaaatg tcaaactatt
    18421 ctaagtttgt aagtggcgta aatcgttaaa ttatcttgct gtgggtattg aaaagtgcta
    18481 tgtatgatcc ttaaactcag gaggggtgca tgggaatatt taactccacg aatccacatg
    18541 tgcgccaaga ccaattaata atctatgtct ttaattctta gtcggacctc ttttttcccg
    18601 ttccacacgt taaattctgg tgtaaaagcc atatcaattc tatttggtac ttttattggt
    18661 tcttttagat taaaccagac agcatcaaaa accctgtctt tctgtttgag cttaagactt
    18721 aaatgcttct ctttgaaaag ccgctcggaa atcacttcaa gcgacttcga taaaagtacc
    18781 ggctctgggt ttccaatgcc aaatggggca agaagctcaa gctccgagac aacttcatcc
    18841 gtaatctcat ttaaatccac aacatcgtca atcttaatca tagaaacaaa atcacccgct
    18901 gactccgata tagctctttc aaatcttttt ctaaactcat caatcctttc ttcctttata
    18961 gaaatacccg ctgcaagctg gtgtccccca aattgctcaa acaattcttt acattctgaa
    19021 agagccgcat aaatatttat tccctcaatg ctccttcccg accctttccc aacaccgctt
    19081 tcttgaatag atattagaat cgcaggcttt tggtatcgct caaccaattt tgtcgccacg
    19141 atgcctatca cgccggcgtg ccagttagga gatgaaagaa caagtgagtt agatttaaga
    19201 aactctgggt ttgactcgat ttgggaaacg gtatcaagaa aaatatcttc ctctatattt
    19261 tgtctttttg agttctcttg gtttaggatt tttgcaagtt ctcttgcttg ttttaaatcc
    19321 tgtgagatta gcaattcaac tgctacacca gcgctttgaa gccttcctgt tgcgtttatc
    19381 cttggcccta acttgaatcc gatgtcatac gaatttacct caccttcaat accgcttacc
    19441 tcctttagcg caatcatacc tgggcgctta gggctcaaca tccttttcat accctctttt
    19501 accaatattc ggtttacgtt cgtaagcgat gcgcagtccg caaccgttcc tagagcgaca
    19561 aggtctagaa aatcaccaag attgggctca ttcctgtcgt gagccgtctc gcctgagctc
    19621 gacgcaggct caggacgcgc ctttctaaag aagcccgatt ctcttagagc tcttctaagg
    19681 gcgatcgcta gattgtaaat cactccaacc cctgcgatct cctttccggg atatgagcat
    19741 cctatttgat gaggatttag gaccgaaacc gcctctggaa ttgtttcagg aggctcgtga
    19801 tgatccgtga cgatgaaatc tacatctaat ttttttgcgt cctgaacctc tttccatgct
    19861 gtgattccac agtcgcctga gattatcagt gagacaccat cattttttaa tttttttatc
    19921 gcttgaagat ttaccccgta accttcctta agtctgtcac gattatagta agtaacattt
    19981 acgcccaagt tcttcagtaa ggtataaaaa agggatgtag ctgtaacgcc gtcaacgtca
    20041 taatctccgt aaatcgcaat tttttctttc ccttgaatag ctctttcaat tctttctaca
    20101 gctttatcca ttccccgcat aagatacggg gagggaagat caaaaagggt gctttttaaa
    20161 aataactcgg cctcagcttc gtctttaatc cctcggttta ttagtacttg agcagtgatt
    20221 gatgatattc ctagagcctt gactagcttc tctttgagtt tgggattgtg cttttctata
    20281 acccagtgtt ttttcattta tgatttacga agatcccacc cctaaagata ggcggggttt
    20341 tccaaccccg agcgcgaatg ctatttagcc actaatagac acggatttac accaataaat
    20401 gatgtacgtt gcatgatggg taattgatga aagtttttac atgcgtcttg aatcctgtat
    20461 cgtgtatcct gcatcttgat tatcttcaat cgtgctcatt tgtgtcaatt cgtggcttaa
    20521 tgattactct tctttaattg gcttacaagc cccctaagcc ctagtatgta gctatacact
    20581 ccaaatcctg atatgactcc tgatgctacg ggagatatga aagacttctg cctgaactct
    20641 tcgcgtctgt aaacgtttga gatgtgaact tcaacagttg gaattcccgt agaggaaata
    20701 gcatctctta ttgcaacact tgtatgagtg tacgcggcgg gatttattat tattccatca
    20761 aatttctttg tcgcatcttg aattttggtt acaatttcgc cttcagaatt agactgaaaa
    20821 gattcaacct gaaccctgag tgccttggct tcttttctaa taagtgaatt aatttcctcc
    20881 agggttgtct tgccataaat ttcaggctca cgcttgccaa gaaggttaag atttggtcca
    20941 tgaattacga gtattttcat ctctgattgg gtttacactc tttcagatga tattacttta
    21001 gaataaggag tggagtcaag aaattttgga aggatctttt tgaaatgaag aacactgtaa
    21061 catgttgata ggtatcagcg ttttaacaga cattcccttc tgtcgttgga acgacagtgt
    21121 ggactaacag tgtggactaa tggtgtcatt gcgagcggag cgcggcaatc tcgtttttag
    21181 atagcttatc ctccttcgcc ttgctctggc tacggcgcac ttctcctagt aatgaccaaa
    21241 ggagttgatt ttttaaacgc taatactctt gagcttgcta caggtttcta ctgtcattgt
    21301 aatgaccaga ggcattgttc atggaaggct aaagccctcc actacaattc ttgttttgcg
    21361 atggattcaa aatgtagcgg agacctgtag gtctccacat gaagattgaa acgaagatcc
    21421 cttcccctta tgcgacgaga ttctgcagct taatgcatgg tgtttcattg tggtattact
    21481 agaaggaggc gagctttaga ttaagtagcg cagagatgtc cttataagag ctattagcgt
    21541 atacatcgtt tagataatgg tagtatgaga catcgaatga tagactgtgc ttcccaaatg
    21601 acgatttagc taacgtccaa aaaaggccga ttgttccatt gagggcgtta gtatcagtgt
    21661 aaccatcgtt gcttttattt attgagtagg agccgtagag tgacaagttg aaatcatcca
    21721 atagaggata atatccgaac catattgagg ccgtaggagt gatgtaacgc gctccgcctc
    21781 ctgaccacac atatctctca tccccaaggc ttaaggtggg gcttatagtt atagagctaa
    21841 ctggacgata tgaaccgctt agctggtaga agaaagcgat gctgttcgaa tctgaaaaga
    21901 atttgtcact ggagattgta gatgttgaag aaactaaaat atcccaattc ggtctagtgt
    21961 agtatagcgt agcgccgaag ttatccagcc agtttttttg aggttgatac ccgttgggtt
    22021 catatacact ccttgagtaa ccatgactat aatagatgga aagcaaaggc atgtttggaa
    22081 ttgcaacgtt aagcgtgata ccaccttgcg ttttcttgat tcggggtttg ccttgattat
    22141 cagcgacatt gtctacaaaa tctgaaaaaa gggtagtaat attgagtaca cccatactac
    22201 gttttagcca taagctgctt ccttcttgat cgctttttat tttccttcag attactttac
    22261 ctaccccatt tctaacatct aggtaatgga ctccatagtc aaatccgccc aagcttcctt
    22321 ttgatccgaa gcgaatgagt aaaggttggt attctatgaa tccctcccta taatcaaaat
    22381 cttgatatgg actatatatc atttcccctt cgccaactaa cttaccacca aggttgctca
    22441 aagttagttg gaagtttaag aatccccacc tttctacatc tcctagaaat ttaatcgatt
    22501 taacttgttg atgtgactgg gaaacattta ctacaaaaaa tcctttgttc tgagttgaat
    22561 tcgatcccta tgtattccca accggataat taatgtcctt accatcggag tttgtagatt
    22621 cgtctttctc tggaggggta taatactttg taaagtcaag ctgagcctga gatggatgaa
    22681 ttttacttcc ttcttcttgc ggatgaatta tttaccgctc atttgttggc gattcttgta
    22741 tttgaggggt ctcgatggag ttatcaaagg aaatagaagt gtcatggatg atatcgaaaa
    22801 caatacgtgg aggagcactc aagcaaaaat gctttttaac aatgcctcct ttcttcagtg
    22861 tgatttcagc cttgattttg gtaggtgtgt ctataatact aacttcctgg acgacggggt
    22921 catgaaccca aaatactaca tgtgactgcg tcgtatgaag cttgccaaac tctacaacaa
    22981 ttttgaaagg atctttttcc ggaatgattt cataaggtgt gtccttatcg aattcaaata
    23041 ccacacgtga aaacttccca tgattgccaa cacggacaga ataaagctga ggctcggtat
    23101 tcccgtattt cggcgagaac cagagaaaaa ggagtaccac cgagatccat ataattcggg
    23161 aaggcataag cctgcctcct aagaggttag attgtttacc aaattaaaag tggaaaacgc
    23221 taccttggaa tttcgataag gtagtctatt ccaacttact cctttgtttt attccctttg
    23281 caacgattat agttcaagcc ggagagaaaa gtcaaatgaa atttttatgt aatacaaatt
    23341 gctttgttgc aagcatcgtt attctacaaa ctgggtaggc aacgagttcc ttcgtcagaa
    23401 ctcaggacag gctcgctcgt tgctgctcag cccaccagta agcctcgttg cctacccaaa
    23461 tacatagggt agcccgccgg tcttgctggc gggattacat tcataagtgt gttatttcaa
    23521 gatgaaaatg gtattaacat agtctaaaga agggggcaaa tcttttctgt taaaacagtg
    23581 tataaataaa tggagcgatt gctgaactct gtgcaaatac gataagaagc ccgaaaatta
    23641 ataacaccaa tatcatgggc gtgagccacc accacttgtg ctggaggaag aattgtagaa
    23701 gctcgccggc aatacccatc ctattagtca agccgtctag aaagcgcatt tgtgatacct
    23761 ccttgggtta gaatacaaga cggaagaccg aagaataatg accggagaca ggagcctttg
    23821 agcagttcct tccgcatttg ctttctattt caaacttttc cgaagggctt gcaacttcac
    23881 gaataaatta tgcccagcat ctcggatagc aaccaaatct tttgaggtta aatagctacg
    23941 ccgctcaatc aagtcccaac aagcaacggt ctccaagtaa gaccgtagag ctagtcctaa
    24001 gaagcggcgt tgctatttat cggtctgtcc tgttgaccct tcggctatgt ttaatacaat
    24061 tgatgtagcg gctcgttcca cctgtgaccg gagattgaaa cgctcacttt caggtaaacg
    24121 tcggctcaag tcgtacacag catttacata ttccaaggct aacttgtata cctccaaccg
    24181 ttgaaactta tacatcccgt ctcctatctc ccatcacccg tctcccgtcc tccgtcttca
    24241 acccgtcttc tcaattatat aatctcccag caccaaaaca tccattccac tcctgctgaa
    24301 tgtaagaaat gactccttgg gggtgtttac tataggctca ccttttagat taaacgaggt
    24361 gttcaacaca actggcacac ctgtagcctg ctcaaaggtt tcaatcagat tgtagtatct
    24421 tggattcact tctttctgaa ctgtctggag ccgtcccgta ccatctacgt gagtgatggc
    24481 tggtacaaca tcttgtttct cttttcgcac atctactaca tatagcataa acctggctgg
    24541 gtaatgccta gccgaatggt ctagactgaa gtagtcttgt gttcgctcta caagcaccga
    24601 aggagcaaat ggacggaatg gctctcggaa tttgatctta acgttgacta tgtctttcat
    24661 atccactcga cgtggatcag cgaggatgct acgattccct aaagcccgtg gcccccactc
    24721 aaatctcccc tgatgccaac cgatgacctt accttcttga agccgatcta caactcggtt
    24781 gatcaatttc tgctcatcct cgatcctttc gtatctgatg ccgttatctg ttaggaactt
    24841 ttcaattccg caagggctgt gttcttctcc ccaataggca tgctccatta taaaatttcg
    24901 tggtttacct agaaccatat gaaaaccaaa cagggcggcg ccaatagccc caccgccgtc
    24961 gccggctgat ggctgaatgt aaatttcttc aaaaggagtt tctttgagta ttctcccatt
    25021 agctacacta ttgagagcca ctcctcctgc catgcaaagc cttttaaggc ccgtttcctt
    25081 gtatgcactg tgagccattt taagtagaac ctcttcggtc acggcctgaa tgcttgcggc
    25141 tacatcggca taatacttgt tctcctgcgc taattcttca taatttgacg gcttttctcc
    25201 aaaataagaa gggtagccac tttctttcgt gaaaaagtga gatttaggat cacgtggaga
    25261 accaaatagg ccctcaaatt ttctattaaa cgttttctca gaagaataat gaaatgaaaa
    25321 atagtccata ttaagctcaa aactaccatc gctgctaacc tgaattagct tatatacctt
    25381 gtccatatac cttggtgttc caaatggagc catccccatt actttgtatt caccctcatt
    25441 tacctcgaat ccgagaaaag cagtaaaagc gctataaaga agccctagag aatgtgggaa
    25501 acgaatctcc tgaaggagct ttatatttgt accctttcca ataccaagcg ttgcggtcgt
    25561 ccactctcca acaccgtcaa ctgttagtat tgccgcctcg tcaaaaggcg aacagaaaaa
    25621 agcgctggca gcgtgagaca agtgatgttc actgaaaaga atttttgaag ctggcacacc
    25681 tatcctcttt tgtagtaggt gcttaatcca tagcttatcg ccaaaccagg taatcatagc
    25741 ctcccggaat aatcgaaggg agtgagggaa ggtttgcata gtggaaagaa gaagcctctc
    25801 gaatttaacg aacggcttct cgaaaaagac tacataatcc agttcaggtc ctttaattcc
    25861 cccaattctt aggcagaaat caatggcatt ttgagggaac tcgtaatcat gcttcttcct
    25921 agtgaatcgc tcctcttcgg ccgcagcgat taactgtcca tcacgtatca acgccgccgc
    25981 agcatc
//
LOCUS       DATDRL010000112         9245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1586241, whole genome shotgun sequence.
ACCESSION   DATDRL010000112 DATDRL010000000
VERSION     DATDRL010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9245
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586241"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..315)
                     /locus_tag="VI935_03785"
     CDS             complement(115..315)
                     /locus_tag="VI935_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24755.1"
                     /translation="MDISKARAVSIEVGMQNSGLGVALARTHFGVLAALPSAMFSVWH
                     NISGSVLAWWWRRKDANYLGTN"
     gene            complement(391..918)
                     /locus_tag="VI935_03790"
     CDS             complement(391..918)
                     /locus_tag="VI935_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HLE24756.1"
                     /translation="MFGMGITLSGEDFKRIFQRPQDVLVGFCVQYGVMPVTDFALAKI
                     LRLEPLVAAGVVLVGSCPGGTASNVITYLARGDVALSVTMTSVSTIFSPAFTPLFTYL
                     FAGQWIPVPVLKLFISTAEIILFPVVLDLGIRGLFKERVDSAAQVLPLVSSLTIIFIV
                     GVIVAANAESLRGIG"
     gene            1248..2090
                     /locus_tag="VI935_03795"
     CDS             1248..2090
                     /locus_tag="VI935_03795"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HLE24757.1"
                     /translation="MKFGAHVSIAGGIANAPSRAYKLGCECFQIFSRSPQGGKSPGLD
                     KITVVDFLKECSKYNFTHYYIHTPYYINLASQNVGLRDSSVSIIKEEIERASLIGAKY
                     VVTHLGSAKELGESEGIKKVIDGLKETLDSDSHSTKLLIENSAGQGATLGDKLEKLAE
                     ILEKVGQRELGICLDTAHLLASGYDVRTEEAVINILDEFTRNVGLDRLKLIHGNDSKA
                     GLGEKKDRHEHIGKGKIGIEGFRTIVNEPRLKDIDIIIETPLDKVGDDIKNIKSLKGL
                     KKKK"
     gene            2131..2541
                     /locus_tag="VI935_03800"
     CDS             2131..2541
                     /locus_tag="VI935_03800"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HLE24758.1"
                     /translation="MKRLAILALMIAIISLAMSYTSSNQIKELQSEQKQISDDLLKLK
                     QEIAKTWQSAEQPGSAKPVWPKYYDATGVLKSMSENRIVVDEDEIPGFMRAMIMSYEV
                     EKPEQLKGVKEGDKVKFKLKETENDLTVLEIQKQ"
     gene            complement(2861..3856)
                     /locus_tag="VI935_03805"
     CDS             complement(2861..3856)
                     /locus_tag="VI935_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015145806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HLE24759.1"
                     /translation="MHAMVLEAPEKALRAMEVSVPRPGPQQVLIKVNACGVCRTDLHI
                     VDGELPNPKFPLIPGHEIVGTVMKTGDQAEKFFVGDRVGVPWLGYTCSECRYCKREQE
                     NLCDRALFTGYTIDGGYAEYTVADQRYCFRIPNTYSDAKAAPLLCAGLIGYRSYRMIG
                     QNAERVGIYGFGAAAHIIAQVAIYQKKQIYAFTRPGDTVAQEFARRLGAAWAGDSTDL
                     PPEELDAAIIFAPVGSLLPAALRATAKGGTVVCGGIHMSDIPSFPYRILWEERIVRSV
                     ANLMRRDGEELLAIAPKVPVKTEVQVFPLEQANKALDILRHGKIQGAPVLSMSSS"
     gene            complement(3883..4305)
                     /locus_tag="VI935_03810"
     CDS             complement(3883..4305)
                     /locus_tag="VI935_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24760.1"
                     /translation="MEGVTYQDFDLLIQRAGTNYRAQVVNSPAGQAALDFSMPFSDLE
                     LENFPLRVGRPLRGLRRVDSPEVEEAKVFGQRLFSTVFNDEVRACLRSSLDEANRQGA
                     GLRIRLRLTDVPELVDLPWEYLYNLVLNRFLTRRITTS"
     gene            complement(4338..5669)
                     /locus_tag="VI935_03815"
     CDS             complement(4338..5669)
                     /locus_tag="VI935_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HLE24761.1"
                     /translation="MGKKYSRGDQSSISLFKAPKSSIPLAERIRPRDFSEFVGQDHLV
                     GPEATILKMIESGNIRPMILWGPAGTGKTTLARIIANRLDADFFQVNAISSGVKEMRE
                     IISHAKESLELGKRTILLIDEIHRFNKAQQASLLQSVEDGTLILIGATTENPSFEVIS
                     PLLSRCHLYILGPLAENYLENILNRALTEDEFIRNIVLSKEAKKELIRLCGGDARVML
                     NSLELAVSLSGVEMPIKIDKDQIREAYQVRHYKYDKGGEEHYNTISAFIKSVRGSDPD
                     AAVYYLVRMLEAGEDPKFIARRLIILASEDIGNAEPYALTLATACFTAVNYVGMPEAQ
                     LILAQTTTYLASCPKSNASCVAIGKALADVREKPYIPVPLHLRNAPTDLMRELEYGKD
                     YKYAHDYDGHFVEEQYLPDELKDKIYYDPTHIGKEAVLKKYLERIWKKRRE"
     gene            5917..6039
                     /locus_tag="VI935_03820"
     CDS             5917..6039
                     /locus_tag="VI935_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24762.1"
                     /translation="MEIELLKQLCEAPGLPGAEEPVKEIVIASLKEFADMDMGN"
     gene            complement(6233..6547)
                     /locus_tag="VI935_03825"
     CDS             complement(6233..6547)
                     /locus_tag="VI935_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013798425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HLE24763.1"
                     /translation="MKTIEEIRKVLAEHKEGLKEKFKVKRLGIFGSYARNEQREDSDL
                     DLLIAFEEKKFIGGWEFIGLTMDLEEFLENTFGIKVHLATERQAMNADKWEYVKKESI
                     YV"
     gene            complement(6618..7016)
                     /locus_tag="VI935_03830"
     CDS             complement(6618..7016)
                     /locus_tag="VI935_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017292745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HLE24764.1"
                     /translation="MKKDNDILELLKKAKSSLKAAKNLFEDGFYDFSASRSYYAMFYA
                     AEAVLLTENLSFSRHSAVIGAFGKEFIKTKIFPQKFREYLVSAFDIRQLGDYGAPGSV
                     SKGKAQNLLERTKEFIEIIKEYLIKEGYRV"
     gene            complement(7013..7342)
                     /locus_tag="VI935_03835"
     CDS             complement(7013..7342)
                     /locus_tag="VI935_03835"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLE24765.1"
                     /translation="MKRTLKDIQPILKAAKERLQKIYGERLKQTILYGSYARGEADDG
                     SDIDLIIVLENVIDPVSELEKCSKEIHELGFLYDTVVSIIPIDANQYKTRRLPIILNA
                     KREGVTI"
     gene            7461..8492
                     /locus_tag="VI935_03840"
     CDS             7461..8492
                     /locus_tag="VI935_03840"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HLE24766.1"
                     /translation="MEIRLLKQLCEAPGLPGAEEPVKEIVISNLKEHTEEIKIDALGN
                     VIAHMPGDGPRLLLDAHMDEVGFMVKHIDQRGFIRVIPLGGIDPRVFYGQRVVVWGKK
                     PLVGMVAAIPPHIINKEHEKEAPEVEECMIDLGLAPEKVSSLVKVGDVVSYFSPFIET
                     EDAIISKAIDDRVGLFIMLEALKQNPKINCDLYVSATVQEEVGLRGANVIVPTYEPDF
                     VIALEGTVSNDLPGVPEHKTLAKVGKGPEIRFTDKYLVADRALSQFIIELAQKRDIPY
                     QITVKKAGSTNATAMQVTGKGSRATVVSVPVRYLHTPSCLAYKKDIEETINLIVAILE
                     DIQYHNLTA"
     gene            8528..>9245
                     /locus_tag="VI935_03845"
     CDS             8528..>9245
                     /locus_tag="VI935_03845"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE24767.1"
                     /translation="MKHIIFFLTLLTFNFSTFLTPIIADEYTENYPNFTKEKIKTKLK
                     EAKAIYFDYYKDSMNLDRSIKILDEIIEVDPKNLQAMIFLSRVWLTYGYAKAPNDEEK
                     IRVFKNGIDVAKKAIELEPNNPDAHFFYVANLGSLGETNGVLRSLFLLPKMRRELELI
                     LELDPNHIYGLGMTGLLYYSLPSFLGGDLHISKIYLRRAMRLDPHLTILKIYLAKTLM
                     KQKKYEEAKKILKEVIEEKNP"
BASE COUNT         2866 a         1923 c         1782 g         2674 t
ORIGIN      
        1 ttccaaacaa attctaaatc aaaaataaat acaagattca ttctagcctc gttttatttt
       61 gattcgtttg tgatttggaa ttttttttgt ttggaatttg tgatttcatt cgtgttaatt
      121 cgtgcctaaa taattagcat cctttcttcg ccaccaccaa gccagtactg agcctgagat
      181 gttatgccat acgctaaaca tagcagatgg aagcgcagca agcaccccaa agtgagtcct
      241 tgctaaagca acaccaagcc ctgagttttg cattccaacc tcgattgaaa cagcgcgagc
      301 ctttgagata tccatcctaa acaaccttgc aatataaaaa ccaagaaaaa gccctattgc
      361 gttgtgaagt acaacagcta ttcccgtttt tcaaccaatc cctcgaagag attctgcatt
      421 tgctgctact attaccccaa caataaatat aattgtgagt gaagaaacta aaggaagtac
      481 ttgggccgca ctatcaaccc tctctttaaa caagcctctt attcctagat cgagtactac
      541 aggaaataga ataatctctg ccgtagaaat aaataacttt aaaactggga caggaatcca
      601 ttgtcctgca aagagatatg taaataatgg ggtaaaggcc ggagaaaaaa tagtagacac
      661 cgaggtcata gtgacagaaa gggccacatc tcctcttgca agataagtaa ttacatttga
      721 agcagtcccc ccgggacacg aacctacaag cacaacccct gctgcaacaa gtggttcaag
      781 tcttaagatc tttgctagag caaagtctgt aactggcatc actccatact gcacacaaaa
      841 acctaccaat acatcctgtg gtctttgaaa tattcttttg aaatcctcac cactaagtgt
      901 tattcccatc ccgaacatta tgactcctat acgcaggagc tattagatat gcaagctttg
      961 cgaagcgttt tggataaaaa taggtaataa tggaaaaagc aattatccag aaggtaaagt
     1021 aacttgtaaa gatgcgattt aaaaacttta gagttttcaa gatgatttta gtagtgaaag
     1081 tacttcaagc aagacaaaac catttatgca aaccctattt ttacaaaata tatactcttc
     1141 aattaatctc aaaagctata tataaatcct tcaagaacat ttcgattgcc aatgggatat
     1201 aacccaagtg agatggataa aatatcctaa tcaattatac ttagcaaatg aaattcggtg
     1261 ctcacgtctc gattgctggg ggaatcgcta acgcaccatc acgggcatat aaactaggct
     1321 gcgagtgttt tcagattttt tcaaggtccc ctcagggtgg gaaatcaccc ggactagata
     1381 aaattacagt tgtggatttt ctaaaggaat gttccaaata taacttcaca cattattaca
     1441 ttcacacgcc ctattacatc aaccttgctt cacaaaatgt aggactaagg gattcctcag
     1501 tctcaataat aaaggaagag atagaaagag caagccttat cggagcaaaa tacgttgtaa
     1561 ctcacctagg aagtgcaaag gaactaggag aatcagaagg gataaaaaag gttattgatg
     1621 gcctcaaaga aaccttggac tccgattcac attcaaccaa gctgctaata gaaaactctg
     1681 cgggacaggg agctactcta ggtgataaac ttgaaaagct tgctgagatt cttgagaagg
     1741 ttggccaacg tgagctggga atatgccttg acacagcaca tctcttagct tctggttacg
     1801 atgttaggac agaggaggcg gtaataaata tcctagatga atttactcgt aatgtagggt
     1861 tagatagatt aaaactcata catggaaacg actcaaaggc cgggcttggc gaaaaaaagg
     1921 atagacatga acacataggt aagggaaaga ttgggatcga gggtttcagg acaatagtta
     1981 acgaaccacg tctcaaagat attgacataa taatcgagac cccgttagat aaggtaggag
     2041 atgatattaa gaatataaaa agtttgaaag gcttaaagaa aaagaaataa tatttcatag
     2101 attaattttt aaacaaggag gtaagatcgt atgaaacgct tagctattct tgctttaatg
     2161 atagctatca tttcacttgc tatgagttac acaagttcta accagatcaa ggaactacaa
     2221 agtgagcaaa agcaaatttc agacgattta ttaaaactta agcaagagat agcaaagaca
     2281 tggcaaagtg ctgagcaacc cgggagtgct aagcctgtgt ggcccaagta ctatgatgca
     2341 acaggtgtat taaaatccat gtcagaaaac cgcattgttg tagatgaaga cgaaataccg
     2401 ggttttatga gagcaatgat aatgagctac gaagttgaaa aaccagaaca actaaagggt
     2461 gttaaagaag gagataaggt taagtttaaa ctcaaagaaa cagagaatga cctaaccgtt
     2521 ttagagattc agaagcagtg ataattagga gttatatcaa ctctatttct caatggtgca
     2581 taaaaaacat gtcatttcaa tgacaacatg ccttaatacc cctataggcg gggttttcga
     2641 acgccgccct ctgttgcggg actagaaagt cccgtctata ggatatggag cacatgctcc
     2701 gcgaatgtgt ttgcaatgac aaaaacagta atgagattgc tttccctcag tttgtttttg
     2761 gggttcggct caacctttta gtcgctcgca acaacatttt gaatactccg atgccggata
     2821 aaattggtat taaatactca gtccgatctg gcatttctga ctaagaagag gacatcgaaa
     2881 gtacgggagc accctgaatc ttaccgtggc gaagtatatc gagagctttg tttgcttgtt
     2941 caagcgggaa aacctgaacc tctgttttca caggtacctt aggagcaatt gctagcaact
     3001 cttctccatc ccttcgcatt aggtttgcca ccgagcggac aatgcgttct tcccaaagaa
     3061 tgcgataagg aaatgagggg atatcgctca tgtgaatgcc accgcagacg acagtgcctc
     3121 cctttgctgt tgctcgcaag gcagccggta acagcgaacc aacaggagca aagataatcg
     3181 cagcatcgag ttcctctggt ggcaaatccg tcgaatcccc tgcccacgca gcaccaagac
     3241 gcctagcaaa ttcctgggca actgtatccc cgggtcttgt gaatgcataa atttgttttt
     3301 tttggtaaat agctacttgg gctatgatat gagcagcagc accaaaacca tatattccaa
     3361 cgcgctccgc attttgccca atcatacgat aagatctata cccgataagc ccagcgcata
     3421 ataaaggtgc ggccttggcg tcactgtaag tattagggat acgaaaacag tatctttggt
     3481 ctgcaactgt atactccgca tacccaccgt caatcgtata accagtgaac aaggcgcgat
     3541 cacacaggtt ctcttgctct ctcttacagt aacggcattc gctacatgta taaccaagcc
     3601 atgggactcc aaccctgtct ccaacgaaaa acttttctgc ttgatcccca gtcttcatca
     3661 cggtgccaac gatctcgtgt ccaggaatta aaggaaactt tggatttgga agctcgccgt
     3721 caacaatatg caaatctgtc cggcaaaccc cgcatgcatt aacttttatg agcacctgct
     3781 gaggaccagg tctaggaacc gatacctcca tagctctaag tgccttttcc ggcgcttcaa
     3841 gcaccatcgc atgcattttt tcagggatgt ccatttcata ccttagctcg tagttattcg
     3901 acgcgttaga aagcgattaa ggaccaagtt atatagatac tcccaaggca ggtcgacaag
     3961 ttcaggaaca tctgttaggc gcagacggat tcgtaatcca gcaccctgac ggttagcttc
     4021 atcaaggctg cttcggaggc acgcgcgtac ctcatcattg aagactgtgg aaaaaaggcg
     4081 ctgtccaaaa acctttgcct cctcaacctc aggtgagtca actcttctta aacctcgtag
     4141 gggacgacca acacgcaagg gaaaattctc aagttccaaa tctgagaatg gcatgctgaa
     4201 atcaagagca gcttggccgg ctggtgagtt aactacctgg gcacggtaat tcgttcctgc
     4261 tcgctgaatc agtagatcaa agtcttgata ggtgacacct tccattgcga tatcctgttt
     4321 aaaccgagat agttaattta ctctcgtctc ttcttccaaa tcctttcaag atatttcttt
     4381 agaaccgcct ctttcccaat atgagttggg tcataatata ttttatcttt aagttcatct
     4441 ggcagatact gctcttcgac aaagtgaccg tcataatcat gtgcatattt gtagtcttta
     4501 ccgtattcaa gctctcgcat taaatccgtt ggagcatttc ttagatgcag aggaactggg
     4561 atgtatggct tttcgcgaac atcggcaagt gctttcccaa ttgcaacgca tgaggcatta
     4621 cttttaggac agcttgcaag gtaagtcgtc gtctgtgcca gaattaattg agcttcaggc
     4681 ataccgacat aattgactgc tgtgaagcac gcagttgcaa gcgttaaggc gtaaggttct
     4741 gcatttccaa tatcttctga tgcgagaatg ataagacgac gcgcaataaa tttagggtct
     4801 tcaccggctt cgagcatgcg tacgagataa tatactgcag catcgggatc gcttcctcgt
     4861 acgcttttta tgaatgcaga aattgtgttg tagtgctctt ctccgccttt gtcgtattta
     4921 tagtggcgga cttggtaagc ctcgcgaatt tgatccttat caattttaat cggcatctct
     4981 acgccactta gagaaacagc taattctaac gaattcagca ttacccttgc atcaccgcca
     5041 catagacgaa taagctcttt tttagcctct ttactaagca caatattcct aatgaattca
     5101 tcttcggtta aggccctatt taaaatattc tcaaggtagt tttctgctaa tggcccaagt
     5161 atataaagat gacacctgga aagtaatgga gatatgacct caaacgatgg attctcagta
     5221 gtagcgccaa taagaatcag tgtgccatcc tcaacacttt gaagaagtga ggcttgctgt
     5281 gctttattga agcgatgaat ttcgtcaatt aataaaatcg ttcgctttcc tagttcaaga
     5341 gactctttag catgagagat aatctcacgc atttctttga caccggagga aattgcgtta
     5401 acctgaaaaa agtccgcatc taacctattg gcaataattc gtgcaagcgt agtcttgccg
     5461 gttcccgccg ggccccataa aatcatcggg cgaatattac cgctttcaat catctttaga
     5521 atcgttgctt cagggccgac aagatgatct tgacctacaa actcactaaa atcccttggt
     5581 cgaattctct cagccagagg tatagaggat tttggcgctt taaaaagtga aatgctactc
     5641 tgatctcccc ttgaatattt cttacccata tccctatata aagaagatta gagtgagatg
     5701 aaagggaaat caataacgag ggcttttctt taaaattcct cttgcaggtt ttaatttgcc
     5761 gtcgctctta gagacccctt cctttttacg tagtgtacga agaggatgat ttttcttctt
     5821 ggtttgcttt ctctttaaaa actctacatt gaagtgtaaa acaattcccc ttactgtagc
     5881 aaatctctta ttgtttgtat ataatcaaaa tttattatgg aaatagagct tctaaaacag
     5941 ctttgtgagg cgccggggct tcctggggct gaagagcctg ttaaggaaat cgttattgct
     6001 tctctaaaag aatttgctga tatggatatg gggaattgat cacagagtct ataaaaactt
     6061 ttgacggagt cttccaaagc cctctgctag taaccgcaga gtctcttggt tttgaagaga
     6121 gttttcaaaa accaaatgaa ttccatttga caggcaaagc gtcaaaccct taaatcttca
     6181 attatgtcct gtggatttaa acttaccatc catctacgtt tttctacatt cttcaaacat
     6241 aaattgattc ctttttcaca tactcccact tatcggcgtt cattgcttgc ctttcggttg
     6301 caagatggac ttttattcca aatgtatttt ctaaaaattc ctctaaatcc atcgtcagac
     6361 ctataaactc ccaccctcct atgaactttt tttcttcaaa agctatcagt aaatctaaat
     6421 cactatcctc tctttgttca tttcttgcat aggagccaaa gattccaagt cgttttacct
     6481 tgaatttttc cttcaacccc tccttatgct ctgcaaggac ttttcttatc tcttcgatgg
     6541 ttttcataca tcaccaccaa atcaaaggct taataaacct atagtccaga ttctccatgt
     6601 tcaattgccc accatcttca aactctatag ccctctttta ttaaatattc cttaataatc
     6661 tctataaatt cttttgtccg ttctagcagg ttttgggctt tccctttact tacggaacca
     6721 ggagcaccat aatcacctaa ttgtcttata tcaaaagctg agactaaata ttcacgaaat
     6781 ttttgaggga aaatttttgt tttaataaac tcctttccga aagcaccgat aaccgctgaa
     6841 tgtctagaga aagatagatt ctcagtcaat aatactgcct ctgcagcata aaacatagca
     6901 taataagacc tgcttgcaga aaaatcataa aatccatcct caaataaatt ctttgctgct
     6961 ttaagactag actttgcctt ctttagaagt tccaaaatat cattgtcttt cttcatattg
     7021 taacgccttc cctcttggcg ttaagaataa tcggtaatct ccttgtctta tattgatttg
     7081 catcaatagg gattatggat acaacggtat catataaaaa gcctaattca tgaatttcct
     7141 ttgagcactt ttctaattca gagactgggt caattacatt ctccaaaaca ataataaggt
     7201 caatatccga gccatcatca gcctcccctc gagcatagga accatataaa atagtctgct
     7261 ttaacctttc tccataaatt ttctgaagtc tctcttttgc cgccttaaga attggttgaa
     7321 tgtctttgag agttcttttc atatcttcac ctttagacct gaaaggtatc atttccaaaa
     7381 aatccgctaa tattgaactg taaatcaatt cctatgatat tagcaaaccc ttctttgtta
     7441 gtatataatc aaaattcatt atggaaataa ggcttctaaa acaactctgc gaggcgccgg
     7501 ggcttcctgg ggctgaagag cctgttaagg aaatcgtaat ttctaatctt aaagagcata
     7561 cggaagaaat taaaatagac gccctcggca acgttattgc ccatatgcca ggtgatggtc
     7621 caaggcttct tttagacgcc catatggatg aagtcggttt catggttaag cacatagacc
     7681 aaaggggatt tataagggta attcctctag gtgggattga tccacgggtt ttttatggtc
     7741 aaagagtagt tgtgtggggt aaaaaaccac ttgtaggaat ggttgctgcc atacctccgc
     7801 acattataaa taaggagcat gaaaaggagg cgccagaggt tgaggaatgt atgattgatc
     7861 ttggactcgc accagaaaag gtttctagtt tagtaaaggt aggcgatgta gtctcatatt
     7921 tttcaccctt tatagaaact gaagacgcca taatctctaa agcaatagat gacagagtag
     7981 gtctctttat tatgctagaa gcgcttaaac aaaacccaaa aataaattgc gatctttatg
     8041 tttctgccac cgttcaggag gaggtagggc ttagaggagc aaatgttatt gtccctactt
     8101 atgaaccaga ttttgtaatt gcccttgagg gcacggtttc taacgacctt cctggtgtac
     8161 ctgaacataa aactctagcc aaagttggga aaggaccaga gataagattt actgataaat
     8221 atcttgttgc tgatcgagct ttgagccagt ttataataga gcttgcacaa aagcgcgata
     8281 tcccatatca aataaccgtc aaaaaggctg gtagtaccaa tgctactgcg atgcaggtta
     8341 ctggcaaagg gagtagagcg acagtagttt ctgttcctgt tcgttatctt cacaccccaa
     8401 gttgtttagc ttacaaaaaa gatattgaag aaactataaa tcttattgtt gcaattttag
     8461 aagatattca atatcataat ttgacagcct aaagggtttc ggcttcgtcc atatttactt
     8521 caattgaatg aaacatatta tattttttct taccctccta acgtttaact tttctacttt
     8581 tttaactccg ataattgctg atgaatatac tgaaaattat ccaaatttta ctaaagaaaa
     8641 gattaaaacc aagctcaaag aggcgaaggc gatttacttt gattactata aggattctat
     8701 gaacttggat agatcaataa aaatcctaga tgagattata gaggtagatc caaaaaattt
     8761 acaggctatg attttcctct caagggtatg gctcacctat ggatatgcaa aagccccaaa
     8821 tgatgaggaa aaaataaggg tatttaagaa cggcatagat gtagcaaaaa aggctattga
     8881 acttgagcca aataacccag acgctcattt tttctatgta gctaatcttg gttcgttggg
     8941 agaaactaat ggggttttaa ggtctctttt tttgttgcct aagatgagaa gagagttaga
     9001 actaatactt gaactagatc caaaccatat ctatggtttg ggtatgaccg gtcttttata
     9061 ttattctctt cctagtttct tggggggtga tcttcacatc tcaaagatat accttagaag
     9121 agccatgagg cttgacccac accttacaat cttgaaaata taccttgcaa aaactctcat
     9181 gaaacagaag aaatacgagg aggcaaaaaa gatacttaaa gaagttatag aagagaaaaa
     9241 cccct
//
LOCUS       DATDRL010000113        11147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1657193, whole genome shotgun sequence.
ACCESSION   DATDRL010000113 DATDRL010000000
VERSION     DATDRL010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11147
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657193"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="VI935_03850"
     CDS             complement(<1..111)
                     /locus_tag="VI935_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HLE24768.1"
                     /translation="MVEIPEVLKYTKEHEWARVEDDIVIIGITDYAQDALG"
     gene            complement(252..1385)
                     /gene="gcvT"
                     /locus_tag="VI935_03855"
     CDS             complement(252..1385)
                     /gene="gcvT"
                     /locus_tag="VI935_03855"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HLE24769.1"
                     /translation="MNHTALYDVHRGMGAKMIEFSGWMMPLEYIGTRDEHLAVRNSVG
                     LFDISHMGEIEVYGKEASLLCQLVTTNDISKLNNLQAQYTLLCNPEGGIIDDIIVYKI
                     SDEHFFICVNAVNTLKDYEWINSEESKFKAIVVNKSPEYSQIALQGPNSQIVLSSVLK
                     MDFSRLKRFFFQFVKWKDSNLIVARTGYTGEDGFEIFLPWDLAKEIWGEIIENAQSYG
                     IQPCGLGARDTLRIEMGYPLYGHEIEEDKNPFEAGLGSFVKFDKGGFIGKESLLGVIE
                     KGIKKKLYGFEMLERGIPRRGYGIVKGDILLGAVTSGTLSPSLEKPIGMGYLKTDIYF
                     DDEIEIEIRGTNRKAKIVSLPFYKRQGKYPATKAPRRKEEYEF"
     gene            1717..2370
                     /locus_tag="VI935_03860"
     CDS             1717..2370
                     /locus_tag="VI935_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HLE24770.1"
                     /translation="MTKSERIKGFYTALFKRYGSQGWWPAESELECILGTILTQNTSW
                     KNVEKALKNLKCKGLTSIEKLSSVPIHVLAELIRPSGYFNQKAIKIKNLIKFLNEYYN
                     GRIERMFKEDPKKLREKLLGIKGIGPETADSILLYAAKKPIFVVDAYTYRILSRHALI
                     PEDSDYGEIQEVFMDVLPKDTHLFNEYHALLVKVGKEHCRKKPICEGCPLDYDPHTV"
     gene            complement(2810..3163)
                     /gene="rplT"
                     /locus_tag="VI935_03865"
     CDS             complement(2810..3163)
                     /gene="rplT"
                     /locus_tag="VI935_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HLE24771.1"
                     /translation="MRVKGGVASRRRRKRLLKLSKGYWGRRKNLIRRARETVLRALAY
                     AYRDRRRRKRDFRRLWIVRINAAVRPYGLSYSEFMGALRKSGVELDRKTLAEMAVRDS
                     ESFKTVVETVKVNLN"
     gene            complement(3286..3492)
                     /gene="rpmI"
                     /locus_tag="VI935_03870"
     CDS             complement(3286..3492)
                     /gene="rpmI"
                     /locus_tag="VI935_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HLE24772.1"
                     /translation="MGKNKIKTNRSAAKRFYKVSASGKVKMAHGGKSHSNEKKGRKRM
                     RRLRTSSYLEGAEAKRVRKVVPYL"
     gene            complement(3629..4162)
                     /gene="infC"
                     /locus_tag="VI935_03875"
     CDS             complement(3629..4162)
                     /gene="infC"
                     /locus_tag="VI935_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HLE24773.1"
                     /translation="MEGGRIAIVREPEIRVNERIRAREVRVIAADGKQIGVLPVREAI
                     KMAEEQGFDLVEVAPNATPPVCRFMDYGKYKYELKKQAVGKKQRVQLLKEVNFRPNIG
                     EHDLDVKINRIREFLDEGHKAKVRISFRGREITHPELGRGLAERIIERIMDIGLIDSS
                     PKFEGKNLITIIVPKKK"
     gene            complement(4144..6084)
                     /gene="thrS"
                     /locus_tag="VI935_03880"
     CDS             complement(4144..6084)
                     /gene="thrS"
                     /locus_tag="VI935_03880"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HLE24774.1"
                     /translation="MSEIQIKLPDGVIKTFPKGITVGEIFKSLGIRDSVIRAKVNGRL
                     VDFWFELDSDSAMEPVTLNSIAGLDLIRHTAAHVMAEAVQSIFNKDNEKIKVTIGPVI
                     ENGFYYDFDYSKGFVPEDIEKIEGRMEQIIEENKPLIRKKVSREEAIRIFENDGETYK
                     VEIISELPLDEQITIYQQGDWYDLCRGPHAPSTGFVKVFKLLSIAGAYWRGDERNPML
                     QRIYGTAFWNRKDLDDYLTKLEEAKKRDHRRLGRELDLFSIQDEIGPGLVLWHPKGAR
                     IRRVIEDFWRSEHERRGYDILFTPHVARMDLWETSGHLDFYREYMYSPMDIEDIVYQI
                     KPMTCPFHILIYKSKIRSYRDLPLRWAEIATVYRYERSGVIHGLLRVRGFSQDDAHIF
                     CTADQIEKEVLSVLDLTVFFLTTFGFDDYEVYLSTRPEKFVGGVENWERATEALRGAL
                     KAKGLNYETDEGGGAFYGPKIDLKIKDVLGRAWQCSTIQVDFNLPERFNINFVGDDNS
                     RHPPIMIHRAILGSFERFFGILIEHYGGAFPLWLSPIQVRVATIGEGQISYAQDVYET
                     LRKHNLRVERDFRNEKLGLKVREAQLEKIPYLLVIGEKEVDNKTVAPRKRGGKNLPSM
                     KTEDFLNFIEKENDPYTWKEVE"
     gene            complement(6180..6254)
                     /locus_tag="VI935_03885"
     tRNA            complement(6180..6254)
                     /locus_tag="VI935_03885"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6220..6222),aa:Val,seq:gac)
     gene            6469..7257
                     /gene="thiD"
                     /locus_tag="VI935_03890"
     CDS             6469..7257
                     /gene="thiD"
                     /locus_tag="VI935_03890"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HLE24775.1"
                     /translation="MPRALTIAGSDSGGGAGIQADLKTFTAFKVFGMSVLTSITAQNT
                     ESVSGIYDLPPDFVKLQIHAVLSDIGVDAVKTGMLSNEDIISAVAEKIKQYGIKKLVI
                     DPVMRAKSGDTLLKPEAERALIEELLPLAYIVTPNLSEAGALSGIKVSSLEDMREAAR
                     RIKLIGCKFVLVKGGHLKWSHEAIDILYDGKKFHDFSSPRIKTKNTHGTGCTYSAAIC
                     AGLAKGLNAREAIRQAKDYVTEAIRNSFSLGNGHGPLNHFWRFE"
     gene            7404..8261
                     /gene="folD"
                     /locus_tag="VI935_03895"
     CDS             7404..8261
                     /gene="folD"
                     /locus_tag="VI935_03895"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HLE24776.1"
                     /translation="MAKIIDGKMVAEHLRKGIAEEVNNLKRKTGLVPGLAAVLVGDNP
                     ASQIYVRNKRKACEEVSIYSEEYKLPEETAESDLLTLIEKLNHDEKIHGILVQLPLPK
                     QINESRILRTVSPLKDIDGFHPENVGLLVEGNSRFIPCTPHGVIKLLDYYGIEIQGKE
                     AVVVGRSNIVGKPVSLLLLHRHATVTICHSRTKSLGEVTRRADILVAAIGRAKLITED
                     MVKEGAVVIDVGINRLPDGKLAGDVDFENVCSKSSFITPVPGGVGPMTIAMLLWNTFS
                     SARNYKIGH"
     gene            complement(8351..8674)
                     /locus_tag="VI935_03900"
     CDS             complement(8351..8674)
                     /locus_tag="VI935_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24777.1"
                     /translation="MSEIYNVALDMSTAIENIDTTIPCGFIINELVTNSIKRAFRDDR
                     KGEIRIELRSGNNGRYEASGQICTLIVGDNGLGSQEFGFPKDRNIWFAIRMYFDRSAY
                     GGNRA"
     gene            complement(8828..9355)
                     /gene="folK"
                     /locus_tag="VI935_03905"
     CDS             complement(8828..9355)
                     /gene="folK"
                     /locus_tag="VI935_03905"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HLE24778.1"
                     /translation="MPIAFIGIGSNLGNRIENCARAIEEISTSVNIITVSSFYETEPV
                     DKEGQPDFINCMTKIETCLSPLKLLSFLKSLEAELGKKNLEKWGPRIIDLDIIFYEDL
                     IIETNDLTIPHPRAHLRRFVLQPLFEIAPSFVHPVLRKSISELLSEVRCIKKVLKLGD
                     FSIYYSHKSTTYPQI"
     gene            9494..9772
                     /locus_tag="VI935_03910"
     CDS             9494..9772
                     /locus_tag="VI935_03910"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HLE24779.1"
                     /translation="MAVLSAKEIKEKLKGLDGWELKGNEIYKIYKHKTFKDAIAFVNI
                     VSDLAEEVNHHPDFLIQYNKVTLTLSTHSQGGVTDNDISLAKKIDKAK"
     gene            complement(9852..10115)
                     /locus_tag="VI935_03915"
     CDS             complement(9852..10115)
                     /locus_tag="VI935_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24780.1"
                     /translation="MNTVPRIYFYKGKLPLPIIPLVLFVLIVLAIFAILGLFIGVIFG
                     AAMLGFIVLRFLISSKKKQAKPVKENGQTIILKEGDYKVIEKK"
     gene            complement(10223..11146)
                     /locus_tag="VI935_03920"
     CDS             complement(10223..11146)
                     /locus_tag="VI935_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004403334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HLE24781.1"
                     /translation="MKKINSLEKNIQTVIKGKPEAAKATLVAFLSQGHMLIEDVPGVG
                     KTTLAQVLARSTDLSFKRVQFTSDLLPSDILGVSIYDQKTGEFDFKAGPIFANIILAD
                     EINRATPKTQSALLEAMSESKITIDGKTYELPKPFMVIATQNPFEYRGTFPLPENQLD
                     RFSMRVSIGYPDYESEKEILLKFSPEKPWDGIQPVVSREEVLVMQTMVERVHLDESIL
                     DYILTIIKETRNPKVFELGVSPRGAIALKRAAQSHALIEGRAYCIPDDVKEMAVPVLA
                     HRVILKHDRMGGKGREEEALKDVLERVKVPI"
BASE COUNT         3286 a         2477 c         2012 g         3372 t
ORIGIN      
        1 cccaagggca tcctgggcgt aatcagtaat acctattata acaatatcat cctcaaccct
       61 tgcccactcg tgttcttttg tgtatttaag cacttctgga atctctacca tttcttttct
      121 cctttgctag cttcttagta aatatttagc cacaaagacg ctaatgcgct aagtagaatt
      181 atgaagtttt tctttggact tctgtccccg acaaattcat cggggactgg attctggctc
      241 ctgaatctac tttaaaattc atattcctct ttgcgtcttg gtgccttagt ggctggatat
      301 ttaccttgcc tcttataaaa aggaagcgaa acgatttttg cctttctatt agttccacga
      361 atctctattt caatctcatc gtcaaaatat atatctgttt ttaagtaacc cattccaatc
      421 ggtttttcaa ggctaggcga taaagtcccg ctagtaacag cgccgagtaa aatatcaccc
      481 ttcacgattc catatccccg tctcgggatt ccacgctcta gcatctcaaa tccataaagt
      541 ttttttttaa ttcctttctc tattacaccg agaagagatt cttttccaat aaaaccaccc
      601 ttgtcaaatt ttacaaatga accaagccct gcttcaaaag ggtttttatc ctcctcaatc
      661 tcatgtccgt ataacgggta tcccatttca atcctaagtg tatctcttgc accaagtccg
      721 caaggctgga taccataaga ttgtgcattt tctataattt caccccaaat ctcttttgcc
      781 aagtcccaag gaagaaagat ttcaaaacca tcttctcctg tgtatccagt tcttgcaact
      841 atgagatttg agtccttcca tttaacaaac tggaaaaaga atctctttaa tctgctgaaa
      901 tccattttaa gaacactgct caatactatt tgggaattag gaccttgaag agctatctgt
      961 gaatattccg ggcttttatt taccactata gccttaaatt tactctcttc gctgttaatc
     1021 cactcgtaat ctttaagagt gttcacagca tttacacata taaaaaaatg ctcgtccgat
     1081 attttataga caatgatgtc atctataata ccgccctcag gattgcaaag gagggtatat
     1141 tgggcctgaa ggttattcag tttgctaata tcattagtgg taaccaactg gcagagtaat
     1201 gatgcttctt tgccatagac ctcgatctcg cccatatggc ttatgtcaaa gagccctact
     1261 gagttcctta ccgctagatg ctcatcccga gttcctatat attcaagtgg catcatccag
     1321 cctgaaaact ctatcatctt tgcacccatc cctcggtgaa cgtcataaag cgcagtgtga
     1381 ttcatcctag tttatgagtt aacatcggaa tagaaggttg tcaacaacat agataagcaa
     1441 aaaactttcc gtgattctca ttgcgaacga agcccccaaa taaggataag gcgggacttt
     1501 ccagtcccgc tgctagttgt catctcgagc cgtgcgaata tctttcatat ccaaaactat
     1561 atgagtaggc tacgagttcc gtcccattaa cacagtacag gtctgtagcg cccctatagg
     1621 gatagcgatt ccatacccta tgtcaagggt aggtggataa gaatcctcta tttgagacgc
     1681 atatcaaaaa ttaaaatggt atcattagtt tttaatatga ccaaatctga gcggattaaa
     1741 ggattttaca ccgctttatt caaaaggtac ggttctcaag ggtggtggcc tgccgagtct
     1801 gagcttgagt gcattttggg aactatcctt actcaaaaca cttcatggaa aaatgtagaa
     1861 aaggctttaa aaaatctgaa atgtaagggt ttaacctcga ttgaaaaatt atcttctgtt
     1921 ccaatccatg tacttgccga acttattcgt ccctctggtt atttcaacca aaaggcaata
     1981 aaaatcaaaa acttaatcaa attcttaaat gaatactaca atggaagaat agaaaggatg
     2041 ttcaaagaag atccaaagaa acttagagag aaacttctag gcataaaagg catcggtcct
     2101 gaaaccgcag atagcattct tctctatgcg gctaagaaac cgatatttgt tgtagacgcc
     2161 tatacatata ggattctttc aaggcacgct ctaattcctg aagactctga ttatggggaa
     2221 attcaagagg tatttatgga cgtcttacca aaagacaccc acctttttaa tgaatatcac
     2281 gccctattag ttaaggttgg aaaagaacat tgcagaaaga aacctatatg tgaaggttgt
     2341 cccctagatt atgatccaca tacggtttaa aattagcctc cccactctaa ccctccctct
     2401 tgaaggggtg cattatttcg aacgctgtat tctttgaacc caaggcacga acttgttcgt
     2461 gccaaaaata gcacagactt gtcctgaact tgattcagga caagtctgtg cctacaatat
     2521 ttaaacagct tttaattgga aatggtataa aggtaaagtg aagaatgtag cggcgatctt
     2581 cagatcgcca taaagaaaat gccaagcgag gtatggaagg ctaaagcctt gcgctacata
     2641 ggtgaattgg gttaagtatt tgtgtgtaga gggaaccttc gggtttccat gcgtgtcatg
     2701 cccgaatgta ttaatcgggc atccaaaaac gggctagtta catggattcc cgctataagc
     2761 gcgcgggaat gacaataaaa aaggagggtt aaagccttgc gctacttttt taattcaaat
     2821 tcaccttaac agtctctact acagtcttaa aactctcaga atccctcact gccatctcgg
     2881 caagggtttt tctgtctagt tcaacccctg actttctaag cgctcccatg aattcgctat
     2941 atgatagacc ataaggtctt acagcagcat ttatccttac tatccatagc cttctaaaat
     3001 cacgctttct ccgacgcctg tctctatagg cataggcaag tgcacgaagg acggtttctc
     3061 tagcccttct tataaggttc ttccttcttc cccagtagcc ctttgagagt tttaataatc
     3121 ttttccttct ccttctcgat gcaactccac ctttgaccct cattacttct tctcctttag
     3181 ttctcagtta acatactgca aatagtaacc atttagccac ccataaaacg atgcatgatc
     3241 catgatacac gatttatctt gcatcctgta tcgtgtatca tgtatttaaa gatacggcac
     3301 aacctttctt accctttttg cttctgctcc ctcaagatag ctcgacgtac gaagcctcct
     3361 catcctcttt cttccctttt tctcgtttga atggctcttt ccgccatgag ccattttcac
     3421 cttaccactt gcgcttactt tataaaacct tttagcagcg cttctatttg tttttatctt
     3481 gttttttccc attagtattt cctatttgta attattcagc cacaaagaaa ctaaaaaaag
     3541 ttcaagagtt gaaagtttaa aggtttaacg tttaactctt gaactcttaa accttaaact
     3601 ttgtgtcttt atgccttagt ggctcctcct attttttctt gggtacgata attgttataa
     3661 gattttttcc ctcaaacttc ggagatgaat caattaaccc aatatccatt atcctttcaa
     3721 ttatcctttc agcaagcccc cttcctaact caggatgtgt aatttcccta cccctaaagg
     3781 atatcctaac ttttgccttg tgaccctcat ctagaaattc tctaatgcga tttatcttga
     3841 catcaaggtc atgctcgcct atattcggcc taaaattgac ctcctttagt aactgcaccc
     3901 tctgcttttt accaacggcc tgctttttaa gctcgtattt atatttgcca taatccataa
     3961 atctacatac agggggggtt gcattaggcg caacttcaac cagatcaaat ccttgctcct
     4021 ctgccatttt aattgcctca cggactggaa gaacacctat ctgctttcca tcggctgcta
     4081 ttactctgac ttctcttgcc ctaatccttt catttacccg tatctcgggt tctctaacta
     4141 tggctattct acctccttcc acgtatacgg gtcgttctct ttttcgataa aattcaagaa
     4201 gtcctcagtc ttcatggaag ggagattctt ccctccgcgc ttcctcggtg caactgtttt
     4261 attatcgacc tccttttctc ctataacaag tagatatgga atcttctcaa gctgggcttc
     4321 tcttaccttt agtcctagtt tttcgttcct gaaatccctc tctaccctta aattatgctt
     4381 ccttagagtt tcataaacat cttgagcata agaaatctgt ccttccccga ttgtagcaac
     4441 acgaacctga atgggactaa gccacagtgg aaaagcgcct ccgtagtgtt caatcaatat
     4501 tccaaaaaac ctctcaaacg aaccgagtat tgctcggtgt atcataatcg ggggatgacg
     4561 tgagttatca tccccaacga agttaatgtt aaatctctct gggagattaa aatctacctg
     4621 aattgtagag cactgccaag ccctgcccaa aacgtctttt atctttaaat caatcttggg
     4681 accataaaat gccccccctc cttcatcagt ttcataatta agccccttag ccttaagagc
     4741 gcctctaagc gcttcagtag ccctttccca attctctacg cctcctacaa acttttctgg
     4801 tcgggtagat aggtatacct cataatcatc aaatccaaag gttgttagaa aaaaaacagt
     4861 taaatcaagc acactgagaa cttccttctc aatctggtcg gccgtacaaa atatatgggc
     4921 atcatcctga gagaaccccc taacccttag aagaccatgt attacccccg agcgttcgta
     4981 tctgtaaaca gtggcgattt cagcccatct aagtgggaga tctctatagc tccttatttt
     5041 agatttataa atcaaaatgt gaaacgggca tgtcataggc tttatctggt agacaatatc
     5101 ttctatatcc attggggaat acatatattc tcgataaaaa tccaggtgtc cgcttgtctc
     5161 ccataggtcc atccttgcaa catgcggggt gaagagaata tcgtatccac ggcgttcatg
     5221 ttcactccgc cagaagtcct cgataactcg tcttattctt gcccctttag gatgccaaag
     5281 gactaaccct ggccctatct catcttgaat gctaaaaagg tcaagctccc ttccaagcct
     5341 tctgtggtcg cgtttctttg cttcttctag ttttgttaga taatcatcta ggtcctttct
     5401 attccaaaag gcagtgccat aaatcctctg aagcattgga ttcctctcat caccacgcca
     5461 atatgccccg gcaatgctta aaagcttgaa taccttcaca aaccccgttg acggggcgtg
     5521 cggacctctg cagagatcgt accaatcgcc ttgttgatag atagtaattt gctcatcaag
     5581 ggggagttcg cttatgatct caaccttata cgtctctcca tcattttcaa aaatcctaat
     5641 agcttcctct ctcgacacct tttttctgat taatggttta ttttcctcta tgatttgctc
     5701 catccttcct tcgattttct ctatatcctc aggcacaaat ccctttgagt aatcaaaatc
     5761 ataataaaat ccattctcta taacaggtcc aatcgtaacc ttaatcttct cattatcctt
     5821 attgaagatg ctctgaaccg cctctgccat gacatgggca gccgtatgtc taattagatc
     5881 aagaccagcg atagaattga gggtgaccgg ttccatagca gagtctgaat ctaattcaaa
     5941 ccagaagtca acaagccttc cgttcacctt agcgcgaatt acactatctc tgattccaag
     6001 ggatttaaat atctcaccaa ctgttattcc cttcggaaag gtttttatta ccccatctgg
     6061 aagtttgatt tgtatttcgc tcattattag caatctagcc atcagtaatc agcagaatag
     6121 taatagcaat tttttgtttt tgctgatagc tgacggctaa ttactgatgg ctatttccct
     6181 ggtgagcgcg gcaggatttg aacctgcgac ctcttgcgcg tcaagcaagc gctctccccc
     6241 tgagctacgc gctcaacaca aaatcgtaac gattaattct acttagatga aaccctgact
     6301 gtcaagaaca gcccaaggcg tgagccatgg cgtgattcgt catcgagcca tatagaacac
     6361 agagcattta ttgaataaaa aatctctatt tcctctgtgc cctctgtggc aaatattaat
     6421 ttgaaactca cccaaatgcc tgtaatatta aattcgatga gtgaaaaaat tccaagagca
     6481 ttaacaatcg caggttctga ttcaggggga ggggcaggga ttcaagctga cttaaagacc
     6541 tttacagcat ttaaggtgtt tggcatgtca gtccttactt ccattaccgc tcaaaacacc
     6601 gagtcagttt caggaattta cgatttgcca cctgattttg taaaactcca gatacacgct
     6661 gtattaagtg atataggggt tgacgctgta aaaacaggaa tgctttcaaa tgaggatata
     6721 atttcagccg tcgccgaaaa gattaaacaa tacggaatca aaaaactggt aatagacccc
     6781 gttatgaggg caaaaagtgg agatacactt ctaaaacctg aagcagaaag ggcgctaatt
     6841 gaagaacttt tacctttagc ctatattgtt actcctaatc tttctgaggc cggagcgctt
     6901 tcagggataa aggtctcatc acttgaagat atgagggaag cggcaagaag aattaaatta
     6961 atcggttgta aatttgtact tgttaaggga gggcatctca agtggtcaca tgaggctata
     7021 gatattctat atgatgggaa gaagtttcat gatttttctt caccgagaat taagactaaa
     7081 aacacccatg gcacaggatg cacctattcc gccgcaatat gtgcaggact tgcaaaggga
     7141 ctaaatgcga gagaagccat aaggcaggca aaagattatg ttacagaagc tataagaaat
     7201 tccttcagtt taggtaatgg gcacggacca ttaaatcact tttggagatt cgaataatcg
     7261 tgatgcacga tacaggatac acgatgcagg ataaagtaca gatttaagtt tcatgaatca
     7321 tgtatcatgc atcttgcatc attttattaa tgtaagttcg tgtacatttt ggctaaatag
     7381 ttaccaaaag gaacaaaaca gacatggcaa aaataataga cggtaaaatg gttgcagagc
     7441 acctgaggaa agggattgca gaagaggtaa ataatctcaa gaggaaaaca ggattagtcc
     7501 ccgggcttgc ggctgtgctt gtgggtgata accctgcatc tcaaatctat gtgagaaata
     7561 agagaaaagc ctgtgaagag gttagcatct actcggaaga atataagctt ccagaagaaa
     7621 ctgcagaaag cgatttactt acacttattg aaaagttaaa tcacgatgaa aaaatccatg
     7681 gaatactcgt tcagctccct cttcccaaac agattaatga atcaagaatc ctaagaaccg
     7741 tctctccatt aaaggacatc gatggttttc atcctgaaaa cgtaggactt ctcgtcgaag
     7801 ggaattccag atttatcccc tgtaccccac acggcgtaat caaactctta gattactacg
     7861 gtatagaaat acaggggaaa gaagcagttg tagtaggaag gagcaatata gtaggaaaac
     7921 ccgtctcgct tcttcttcta caccgacatg caactgttac aatatgtcat tcgaggacta
     7981 aatcacttgg ggaggttaca aggagggctg atattttagt agctgcgatt ggacgcgcca
     8041 aacttatcac agaagacatg gtaaaagaag gcgcggttgt gattgatgta ggtataaacc
     8101 gtcttcccga cggaaagctt gcaggtgatg tggattttga aaacgtttgt agcaaatcct
     8161 catttattac gcctgtccca ggcggagtag gtcccatgac aattgctatg cttctatgga
     8221 atacattttc atctgcaagg aactataaaa taggacatta gttagtggag gtgctatcca
     8281 cttttttcaa aacccttaat ccaccatcta aactgaaaat gtaaccttaa atgttgttcc
     8341 agagtttcta ttaagctcga ttgcccccat aagctgatct gtcaaagtac attctaattg
     8401 caaaccaaat gtttctgtct ttcggaaatc caaattcttg ggaacctaaa ccgttatctc
     8461 cgacaataag ggtgcagatt tggcctgacg cctcatatct gccattatta cctgaacgaa
     8521 gttctatcct gatttcacct ttcctatcat ctctaaaagc gcgcttaata gaatttgtga
     8581 caagctcatt gataataaac ccgcaaggaa ttgtcgtatc tatattttct atggctgtgc
     8641 tcatatctag ggcaacattg tagatctcag acatgtcttg tggataactt cgaagatatt
     8701 atgtaaaaaa ttattcacta ttagtaccca cttgcgttaa gaaattattt ataacgttgt
     8761 gttttcaata agttagattt aaagaaaaaa aattcctaat tgtggataaa ttatggaagg
     8821 aaatgttcta tatttgtgga taagttgtgg atttatgtga atagtatatt gaaaaatcac
     8881 ctaattttaa taccttcttg atacacctaa cctcacttaa aagctcagaa atagatttcc
     8941 ttaataccgg atgtacaaaa cttggggcaa tctcaaatag gggttggaga acaaaacgtc
     9001 taagatgagc ccttggatga ggaatagtga gatcatttgt ttctattata agatcttcat
     9061 aaaatataat atctaggtca attattctcg gaccccattt ctctaggttc ttctttccca
     9121 attcggcctc caaggattta agaaaggaca aaagtttcaa aggagaaaga caggtttcaa
     9181 tcttagtcat acagtttatg aaatctggct ggccctcttt gtctacaggc tcggtttcgt
     9241 aaaaggatga aacggtaata atatttacag aagtcgaaat ttcttcaata gctcttgcgc
     9301 agttttcgat ccgatttcca agatttgaac ctattcctat aaaggctatt ggcattcgaa
     9361 taagctccaa attaaggcaa atcccaaatc acaagcaccg aacaacaaac aaaaaggttt
     9421 atgatcgtca caaatctcgt gatattatat atcatcatag tcaaccttac tatgaatcaa
     9481 cggaggtaaa aacatggcgg tcttatctgc aaaagaaata aaagaaaaac ttaaaggctt
     9541 agatggctgg gaactaaagg gcaacgaaat ctataaaatt tacaaacata agacatttaa
     9601 agatgcaatt gccttcgtaa atatagtatc agacctagct gaagaggtaa accaccatcc
     9661 agactttctg attcaatata ataaggttac tctaaccctt tcaactcaca gtcagggcgg
     9721 tgttactgat aatgacataa gcctagcaaa gaagattgat aaagctaaat gagtcaggcg
     9781 tgactcgtga atggtggttc gtggatcgag cctcaaatca cgaatcacga tacccgagtc
     9841 acgaataacc attatttctt ctctataact ttataatccc cttctttcag tataatagtt
     9901 tgaccgtttt cttttaccgg cttggcttgt ttcttcttag atgaaataag aaaacgaaga
     9961 acgataaatc ctaacatagc agctccaaag ataacgccga taaaaagacc tagaatagca
    10021 aatatagcaa ggactattag tacaaataaa acaagtggaa tgataggtaa tggcaacttg
    10081 cccttataaa aatagattct aggaactgta ttcaatgttc tcccccattt tcgtgagtag
    10141 tgaattgcgt atagtgagtc gagatcgtgt atcatacata gcagcaacga gttacgaatc
    10201 acaaacccca aatcacaaat acctaaatcg gaaccttcac tcgttcgagt acatctttaa
    10261 gcgcttcctc ctctcttccc tttcccccca tgcgatcgtg tttaaggatg accctatgag
    10321 caagaactgg cacagccatt tcctttacat catctggaat acagtaagcc cgcccctcga
    10381 taagcgcgtg ggattgcgct gcacgcttaa gggctatcgc tccccttgga ctcaccccaa
    10441 gctcaaacac cttaggattt cttgtctctt ttataattgt caagatataa tcaagaatag
    10501 attcatccag atgaaccctt tcaaccatag tctgcataac tagcacctct tccctagata
    10561 caactggttg tataccgtcc cagggttttt ccgggctgaa ctttaagagg atttcctttt
    10621 cactctcata atccggatac ccaatactta ccctcataga gaatcgatct agctggtttt
    10681 ctggaagtgg aaaggttcct cgatactcaa aggggttttg cgtggcaatt accataaacg
    10741 gttttggtag ctcgtaggtt tttccatcta tcgttatctt agattcgctc attgcttcca
    10801 aaagggcgct ttgagtttta ggtgtcgctc ggtttatctc atctgcaaga attatatttg
    10861 caaagattgg acccgctttg aaatcaaatt ccccagtttt ctggtcgtaa atagaaacac
    10921 ctagaatatc cgatggaaga aggtcactcg taaattgaac cctcttaaaa gaaagatctg
    10981 ttgatcttgc aagtacctga gcaagcgttg tttttccaac acctggcaca tcttcaatca
    11041 gcatgtgtcc ttgtgaaaga aatgcaacaa gtgttgcctt tgctgcctcg ggtttcccct
    11101 ttataacagt ttgtatgttt ttctcaaggc tattaatctt cttgata
//
LOCUS       DATDRL010000114         2573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1273338, whole genome shotgun sequence.
ACCESSION   DATDRL010000114 DATDRL010000000
VERSION     DATDRL010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273338"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(422..862)
                     /locus_tag="VI935_03925"
     CDS             complement(422..862)
                     /locus_tag="VI935_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE24782.1"
                     /translation="MNILHSKAAAGLFVGDFAIELAKVDHQVYIFTQAREGDIEDDPE
                     LKVVRFNWNGGEKPLARLNPLNPKELISIISLMRSGKRSFFKLVMEEKIDYCLAMWAF
                     PSGYFAFQAYKECNIPYAIWSLGSDVWTLGRYPIMRWFLKWMTL"
     gene            complement(1227..>2573)
                     /locus_tag="VI935_03930"
     CDS             complement(1227..>2573)
                     /locus_tag="VI935_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24783.1"
                     /translation="LLLLLILCFRPLLMKLLSRHQASTHLPSLYYLPLVLVLTYCALY
                     SYFVEGYYFPRFFLPLFPLIVIFLVVCYSRLLSRLNLAFSHVAVLLAVLIVLPFNFEH
                     FRRGASLELKQDFTSTWASARIPFPFKVGPWYWYFMKKNYSMSWSRAIYEQLKANVNS
                     DARVLITSSIMYPWAGRRTLQMGYYFGDNAIYLIDHTEPLDELISKYRINYVLFTTYR
                     ADKRMLWLRYYRKYQYGGRWVGEELLLGASYGFKPGEYTLERELHALTEFLQRRGAKR
                     LYAKGRVLIWELSDAEGQRQMHRTKHQSSLTYSLTSSTGKSGENIISSNGTSIKVVPG
                     ALRGYLDDMVIRNYLVEFHGWAADVKNSKLPEAILVFVDGKFFYSGRTNVDRHDVVKA
                     YGDAALHKAGFYYAFPLQMFNGNAMVRIFAVTNSVASELRYPGGNQLGIKQKRRRM"
BASE COUNT          736 a          586 c          492 g          759 t
ORIGIN      
        1 atatccttta tatctcgcct tgagatctcg aataacaaaa ttctttatta gttctttata
       61 ctcaattatc tctattatct tttttattag cattttgttt gactaaacaa agttttctga
      121 atcattccct ccgataggta aaaatttcac ctttttcagc ttcagaattc ctctttcttg
      181 tattgttcac tttttgaaca tagtttacaa aaaaatttta aaaaaggtga atctaagatt
      241 aaagtgcttt aatgtgctcg gtaaactcag atttcatgga ttggaattct cttttccatt
      301 ggcccgctgc aaagcaaggc ggttcacagg ttctttttct gtttctttaa tgtttctttg
      361 aacagttcaa atcgtggtaa ttcttcctca tctctctggc ggccagcggc ttttttgctc
      421 tttataatgt catccatttt aaaaaccatc gcattatagg atatctccct aaggtccaaa
      481 cgtcagaccc tagagaccat attgcgtagg ggatattaca ctctttataa gcctgaaatg
      541 caaaatatcc gctggggaaa gcccacattg ctaagcaata atctattttt tcttccatta
      601 caagtttaaa aaaactcctt ttaccactcc gcattaacga tataatggaa attagctctt
      661 tgggattaag ggggtttagt cgcgccagag gcttttctcc tccattccag ttaaatctta
      721 ctacctttaa ttccgggtca tcttcaatat ccccttcacg tgcctgagtg aaaatgtaga
      781 cctgatgatc cacttttgcc aactctatag caaaatcccc aacaaatagc ccagcagcag
      841 ctttggagtg taatatgttc aagggatagg aactagttaa tatacaaatt tttaattatt
      901 cccactataa acgctgtgcc ttatgctagt ccagaagata aagagaaata atcacataat
      961 aacagcttat cttttgccac taaccaaaac aaatttttgc cttctatatg acatattcct
     1021 ctcctcctta ctctttgcca cctctcaatg ttatttgagg actactccat ccggcccaat
     1081 cgtagtctct attaccttta ggaccactag tagttttaaa tacgaaagac accttttgtc
     1141 cagcaaaagc ctttagatca aggttgttat caaaccactt cctgtcttct acattattgt
     1201 tttagggtca atgcatcgtg caaagattac attcttctcc gtttttgttt tatacctaat
     1261 tggttcccac ctggatacct cagctcagat gccacgctgt tcgttacggc aaagatgcgc
     1321 accattgcat tgccattgaa catctgtaat gggaatgcat agtagaatcc cgccttatgc
     1381 aatgcagcat ctccgtaggc cttaaccaca tcgtgccgat ctacattggt ccgtccagag
     1441 taaaagaatt tcccatccac aaaaaccaga atggcctctg ggagtttgga gttttttaca
     1501 tcagcagccc agccatgaaa ctccactaaa tagtttctga taaccatatc gtctaggtac
     1561 ccccgcaaag cacctgggac cacttttatg gatgttccat ttgaagatat gatgttttca
     1621 ccactcttgc cagtcgatga agtaagggaa taggtgaggg atgactggtg tttggttcga
     1681 tgcatctgcc gttgtccttc tgcatcggat agctcccaaa ttaggaccct tccttttgcg
     1741 taaagccgtt tggctcccct cctttgaaga aactcggtaa gggcatgtaa ttcccgctca
     1801 agcgtgtact caccgggttt gaaaccgtag ctggcgccaa gtaagagctc ctcccctacc
     1861 caccgtcccc catactggta ctttctataa taccttagcc atagcatccg cttgtcggcg
     1921 cgatatgtgg tgaaaagtac atagttgatt ctgtacttag aaatcagctc atccaggggc
     1981 tcggtatgat ctattaggta tatagcattg tcgccgaagt aataccccat ctgaagggtg
     2041 cggcgaccgg cccacggata catgatgctc gatgtgatga gcaccctcgc atcggagttt
     2101 acatttgctt tcaattgctc gtatatagct ctcgaccagc tcatcgaata gtttttcttc
     2161 ataaaatacc aataccaagg cccgaccttg aaggggaagg gtattcgagc agaagcccaa
     2221 gtacttgtaa aatcctgctt caactccaag gaagcacccc gtcggaagtg ttcaaaattg
     2281 aagggtagga ctatcagaac tgctaacaac acagcaacat gagaaaaggc caggttcaag
     2341 cgactgagaa gtctcgaata acagacgacg agaaaaataa cgataagagg aaacagggga
     2401 agaaagaacc ttgggaagta gtatccttca acgaaatatg aataaagagc acagtaggtt
     2461 agaaccagca ccagcggtag gtaatacaac gaaggtaagt gagtactcgc ttgatgtcgc
     2521 gagagaagct tcatgagaag cgggcgaaaa cagagaatta gaaggagaag gag
//
LOCUS       DATDRL010000115         3085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3742937, whole genome shotgun sequence.
ACCESSION   DATDRL010000115 DATDRL010000000
VERSION     DATDRL010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3742937"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..747
                     /locus_tag="VI935_03935"
     CDS             289..747
                     /locus_tag="VI935_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24784.1"
                     /translation="MIVETDIFRIEEKLDPIIKEGFIVPFEELGRTYYKIPGEENNNK
                     KSKSIFSLTGFFNSKLKFEPIPERTDMKHILTSRNNYKKLIQEAYVLTLPPNPYTEEE
                     RIKTNLWVERDLKKLSTLDEKRLFLADFIYEKSVGKKKDYFKKRKLIGES"
     gene            complement(855..1457)
                     /locus_tag="VI935_03940"
     CDS             complement(855..1457)
                     /locus_tag="VI935_03940"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HLE24785.1"
                     /translation="MIKLYDYPQCPFCRKVRIVLAEKGIKYERIFVDLRKKEQKKEDF
                     LRLNPYGKVPVLIDDGVVIYESSVINEYLDEKYPNPPLMPSDFAARAKVRILVDFCES
                     HFFPPWYNIYKEITFKNEHERNNDLIKKSKNEINEYLYRLEQELEGQEYLAGSYSLAD
                     IAFTPKIALFETLGIQVDSELKNVRSWVEKIKSRPSYQAL"
     gene            1646..2602
                     /locus_tag="VI935_03945"
     CDS             1646..2602
                     /locus_tag="VI935_03945"
                     /inference="COORDINATES: protein motif:HMM:NF018349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1152 domain-containing protein"
                     /protein_id="HLE24786.1"
                     /translation="MELNLSLEEILRKSTKAILLGIGGGGDIVGTIPTARLLEMFGIE
                     CVLGGLPWERFVYDPIPGPRAFNEIKNIRPLNSTVWFANKDTVTTTGVRFAETEIAEI
                     YGKEVLLIDITKGVEGVVNGLRKAMDELQADLLIGVDVGGDSLAFGDEKGLASPLADS
                     IMISALSELQKNHAALLGVFGYGSDGELEHMELERSLSIVAKEGGLLGAWGITPQILT
                     DLEIALSRVSTEASRIPVECAKGATGKTTIRRGTRMVALSLTSTITFYLSPRVVYEMI
                     SKPARLVSGSKSLDEANDSLRSIGLFTELDLEREIQNVVPTK"
     gene            complement(2866..>3085)
                     /locus_tag="VI935_03950"
     CDS             complement(2866..>3085)
                     /locus_tag="VI935_03950"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HLE24787.1"
                     /translation="GFVLGVGMVKILHLSGVASNVVILQSAMPPAIFNFVLAERYKQD
                     SKVVASIIMAGTLISVITTPIIIAYLIR"
BASE COUNT          972 a          536 c          627 g          950 t
ORIGIN      
        1 taaccgcctc cgatctcaca aagcccggcg attataaata gtagagcaga tttaagaatt
       61 aacatttatg gtcctcctat atctacttgt gtatatgatt ctttaaatag gaattataat
      121 taaacatcct tttaatataa aagatggatg tgctttctat tctattgaca gccccttaaa
      181 ttttatcatt attattattt tattattaga tacacacagg ctgttgattc gtccacattt
      241 cttaagataa tcaaagaaag gggacctctt accatatggg aaatgtctgt gatagtggaa
      301 accgatattt tccgtataga ggaaaagctc gatcctataa ttaaagaggg atttattgtt
      361 ccatttgaag agctgggaag gacatattat aaaatcccag gagaggagaa taataataaa
      421 aaaagcaagt caattttttc tcttacaggt ttttttaaca gtaagctcaa atttgaacct
      481 atacctgaaa gaacggatat gaagcatatt cttacatcga ggaataatta taagaagctc
      541 attcaagagg cttatgttct tacgcttcct ccgaatcctt atactgagga ggagaggata
      601 aagacaaacc tttgggtaga aagggattta aaaaagctat caacccttga tgaaaaaaga
      661 ctcttcctcg cagactttat atacgaaaaa tcggtcggca agaaaaagga ttattttaag
      721 aagagaaaac tcattggaga atcctaactt ccgatttcgg catacaagat ttgccacaga
      781 gaacacagag aaaaaaaaca aaaatatctt tcttttctcc gtgaactctg tggttatgtt
      841 tttaaatcca caatctaaag ggcctgatag ctcggtcttg atttaatttt ctctacccaa
      901 cttcttacgt tcttgagttc ggaatccact tgaataccaa gcgtttcaaa gagtgcgatc
      961 ttgggtgtaa aggctatatc tgcaaggctg taagagcctg ctagatattc ttgtccttct
     1021 agttcctgct ctaggcgata aaggtattcg tttatttcat ttttactttt tttaatcaaa
     1081 tcattattgc gttcgtgctc atttttaaaa gtaatttctt tatagatgtt ataccaagga
     1141 gggaagaaat gagattcaca aaaatccact aaaatccgta ctttcgctct ggcggcaaag
     1201 tcagaaggca taagtggggg attcgggtat ttctcgtcga gatactcgtt aattacagac
     1261 gattcataaa ttacaacacc atcgtcaata agcacaggca ccttaccgta gggattgagt
     1321 cttaggaaat cttctttttt ctgttccttc tttctaagat caacaaagat tctttcgtat
     1381 ttgataccct tttcggcaag aacgattctt acctttctac agaaaggaca ttgggggtaa
     1441 tcgtatagtt ttatcatgtt ggacctcatt gagttattgt tgcatgtatc gttttgaaat
     1501 aatcgcttcg tgcttactta aatatggtat tatattttac tcggaaaagt ctacctggga
     1561 ataaaacgta aaattacatt ctaagcaatt tacagaatta gaagataatt aggataatta
     1621 cgtaaaaagg aggttctatg ttcagatgga gttaaatttg tctctggaag agatcttacg
     1681 aaaatccaca aaggcaatac tcttgggaat agggggcgga ggtgatattg taggaactat
     1741 tcctacagca aggctacttg agatgtttgg tatagaatgt gtactcggtg gtcttccctg
     1801 ggagaggttc gtttatgatc ctatccctgg tccgcgagct tttaatgaga ttaaaaatat
     1861 aagacccttg aatagtactg tttggtttgc aaataaagat actgtgacca caacaggagt
     1921 aaggtttgcc gaaactgaaa tagccgagat atacggaaag gaagtgcttc ttattgatat
     1981 aacaaagggt gtagaaggcg tggtgaatgg tttaagaaag gctatggatg agctacaagc
     2041 agatctattg ataggagtag atgtgggagg agattccctt gcttttggag atgagaaagg
     2101 gcttgcaagc ccgcttgctg actctattat gatttctgca ctttctgagc ttcagaaaaa
     2161 tcatgctgct cttttagggg tttttggtta cggaagcgat ggagaacttg aacacatgga
     2221 attagaaagg tctctaagca tagttgcgaa agaaggaggg cttcttggtg cttggggtat
     2281 tacacctcaa attctcaccg atcttgaaat tgccctctca agggtttcaa ctgaggcaag
     2341 cagaatccct gttgaatgtg caaaaggggc aacagggaaa accactatcc gtcgaggaac
     2401 aaggatggtt gctttatctc ttacatcaac aataacattt tatctctctc ctcgggttgt
     2461 ctatgaaatg atttcaaagc cagcaaggct agtttcgggt tctaaatctt tagacgaggc
     2521 aaatgattcc ctgcgttcaa tcggtctttt tacagagctt gatctcgaaa gagaaataca
     2581 aaatgtagtt cctaccaaat aataagtagt ggttttatga gctatgattt gttaaaaggc
     2641 aataatgtac cagggtaagc aacgagtctt gctcgttgtc tgccaccagc aggactggtg
     2701 gactacccgg agttttttac cgaattagat attcaaagat cattaaaact ccatacaatg
     2761 gaatcataaa ggattcgcta cagttctctt atatcttata tcgaacttaa gctaattagg
     2821 tgcatggttt agctggttac taatgtatgt ttactctaaa ggactttacc taattaaata
     2881 agcaataata atcggggtgg taataactga aatcaaagtc cctgccataa tgattgatgc
     2941 cacaacttta gaatcctgct tatacctttc agcaagaacg aagttaaaga ttgcaggggg
     3001 cattgctgac tgcaggatta ctacattaga agcgacacca cttaagtgaa gaatttttac
     3061 cattcccact cccaaaacga atccg
//
LOCUS       DATDRL010000116         7178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1112517, whole genome shotgun sequence.
ACCESSION   DATDRL010000116 DATDRL010000000
VERSION     DATDRL010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112517"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VI935_03955"
     CDS             <1..399
                     /locus_tag="VI935_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HLE24788.1"
                     /translation="YYGLEEQTKRVFKNGFYFTEDLGKMDEKGYIYILGRKKLFINVA
                     GNKVDPTEVENFLLNHPKIKEVVVLGASDNYGNELVKAVVVPKEDMDSKEIYAYCKDQ
                     VADFKIPRMVEFRKEIPKSPTGKVLREYLK"
     gene            475..732
                     /locus_tag="VI935_03960"
     CDS             475..732
                     /locus_tag="VI935_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HLE24789.1"
                     /translation="MNEEIKNTVKQVLIRKLLHDFAPEDINDDAPLIELGVGVDSVAT
                     LELIVALEEEFKISIDESEVNQELLATVNNISDYIWKLVTA"
     gene            complement(956..1666)
                     /locus_tag="VI935_03965"
     CDS             complement(956..1666)
                     /locus_tag="VI935_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HLE24790.1"
                     /translation="MTIEEIIKESKKRGINLGVNPAKTIRFYTRLGFIPKPTRKKSKE
                     GKVKSTKLYYPEFTLDKLAEIKALKSKGLSLDEIRDSFALEYVQKALQDLLSKADDEK
                     VRELARIIGSKENELEAIIEAPLIYIIEGMSQEEVKKLLTLFCGVGFYALLETQQAIE
                     EFKINDARRALFKAIFYISIAVLRLARTTGDTKLESTASEVYEKVVLEPIRKASGRVR
                     KEFISSLEAHLKEKGDIE"
     gene            complement(1814..2398)
                     /locus_tag="VI935_03970"
     CDS             complement(1814..2398)
                     /locus_tag="VI935_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24791.1"
                     /translation="MKEAFDFKPEIYGRVFSELLKEKRLNPLGPGCPNIKTRSALNKL
                     TIHNAFAPHEVVDANMANACLAAVWVCHDFLLEPHNISQTIRTQTGSYWHGIMHRREP
                     DFYNSKYWFHNVGEHAIFEPLRNGAVQLASNSNARKSTEFLSKQSKWNPIAFIDPCEA
                     CIKGRSPCELLSRQIQQFEWKLLFDYCYWKAIKR"
     gene            complement(2419..2721)
                     /locus_tag="VI935_03975"
     CDS             complement(2419..2721)
                     /locus_tag="VI935_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003390986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HLE24792.1"
                     /translation="MIKHIVIWRLKEFADGRGKEENAKKLKNHLESLKSKIKEIKHIE
                     VGINITISDAASDVVLYSEFDSMDDLEVYQRHPEHMKIVDFANEVRLERRVVDYQV"
     gene            complement(2879..3337)
                     /locus_tag="VI935_03980"
     CDS             complement(2879..3337)
                     /locus_tag="VI935_03980"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HLE24793.1"
                     /translation="MILFANIAGRFSTLAGVALAGIFLTGVHNAWFEVGSFQALWQTP
                     YGQTLIAKLLLLLTLIFLGASNRYISVPLLQQWADDPLIKRRVIYNLFLAGYVTSVKR
                     KLDGTQLARQFMHKVWIEAILIVAVLICAALLQHEVPARHFLNVGREHAM"
     gene            complement(3935..4306)
                     /locus_tag="VI935_03985"
     CDS             complement(3935..4306)
                     /locus_tag="VI935_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HLE24794.1"
                     /translation="MMRTISRVITLCGLMLLCLWTDAAWGHVFPEHAEPEVGATVTVA
                     PTRVRIWFNGALEPAFSTFHVQNSSGEQVDRGDGHGAPFDPTLLEVSLPPLPPGTYRV
                     FWSAVARDGHRTEGDYIFTIK"
     gene            complement(4318..5289)
                     /locus_tag="VI935_03990"
     CDS             complement(4318..5289)
                     /locus_tag="VI935_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24795.1"
                     /translation="MKKLLLAMTFCPVVICMVVLLLMPVSAWAHGFAGKRFFPTTLSI
                     EDPFVSDELSFIFSYLKESGEGDEPPVNSYELEGEYTKRITPHFGITIGDEFRILDPD
                     GEDTEVGFGNLEAGAKYQFLTNAPHESVLSIGVNVEIEDTGNREVEAEPFTTISPALF
                     FGKGLGDLPESLKYLRPLAITGVIGPNFPTDTKTVTTEVDEETGEIEREVERNPNTLS
                     YGFAIQYNLQYLQSFVKDVGLSTPFNRMIVLVEFPFETCLDRGCDGETTGFINPGIIW
                     FGKYIQLGAEVQIPINDRSGENVGVLTIVHFFIDDLFPNSFGRPILR"
     gene            complement(5874..>7178)
                     /locus_tag="VI935_03995"
     CDS             complement(5874..>7178)
                     /locus_tag="VI935_03995"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HLE24796.1"
                     /translation="PNFTALVRKLKPSVVNISTTSLIRNGRAFRFPSPHGKEEDSLQE
                     LFRRFFGDIPQREFRQRELGSGFIISEDGYIITNNHVVEKAKEIQVILEDEEKYSAKI
                     VGRDPKTDLALLKIVPQEKLSPVILGNSDKLEIGNWVLAIGNPFGLGHTVTAGIVSAK
                     GRILGFGDYDDFIQTDAPINPGNSGGPLFNLEGEVVGVTSIIFLRAQGIGFAIPINFA
                     KDTIEQLKGDRTVVSGWLGVTIQDVTPEIAKAMKLPEAMGALVGDVTSGSPADKAGVK
                     RGDFIFEFNGHKINENGELSRTVSAISPGTEVELKLLRDGTEKTLSVKLGVLASENSD
                     SEELQNVQSGLGLTVDELTPEIAKRLGVENESGVILSKVDPGSSADKAGFKIGDVILE
                     INRKIITTSEDYKNILSSLTNGQKALFLVKRKEVILYIAMIF"
BASE COUNT         2080 a         1466 c         1436 g         2196 t
ORIGIN      
        1 tattatggtc ttgaagaaca aacgaaaagg gtttttaaaa atggttttta ttttacagag
       61 gatttaggaa aaatggacga gaagggttat atctacattt taggtagaaa gaagcttttc
      121 ataaatgtag cggggaataa agttgatccg actgaggtag aaaattttct cttaaatcat
      181 ccaaaaatta aagaggtagt agttctgggt gcaagtgata actatggaaa cgaattagta
      241 aaggctgtag ttgtaccgaa agaggacatg gattcaaaag agatctatgc ctattgtaaa
      301 gatcaagtag cagattttaa gataccccgt atggttgaat ttagaaagga aataccaaaa
      361 agccctacgg gaaaggtgct aagggaatat ttgaagtaaa atctattcgg ggattgtggc
      421 tcgtgaatcg tgtaccgaac cacgaaacca gagtcacaaa tcacggataa gcacatgaat
      481 gaagagatca aaaatacggt taaacaggtt ttaattagaa aactgcttca tgattttgca
      541 ccagaggata taaatgacga tgctcctctt attgagttag gagttggcgt tgattctgta
      601 gccacactcg agttaattgt tgcattagag gaagagttta agataagtat tgatgaaagc
      661 gaagttaatc aagagcttct tgcaactgtt aataacattt ccgattatat atggaagctt
      721 gtaaccgctt aacagggcta ataataacag gtttttttat atgctgtcct taaaacccaa
      781 gcctactggg aattcgttat taaagctata atgttattac aattgagttc aaacattacg
      841 gggtaaagct ttaagccaaa attcacactc tatataatac gaaattaaaa ggaggattcc
      901 gttgagttat tttgttataa gttcttacaa atattagctc ttaggaaaga acccattact
      961 ctatatctcc cttttccttt aggtgcgcct ctagagaact aataaattcc tttcttacac
     1021 gcccgcttgc tttgcgaatc ggttcaagaa ctaccttttc atacacttct gaggcagtgg
     1081 attcaagctt tgtatctcca gttgtgcggg cgagccgcaa gacagctatc gagatataaa
     1141 atatggcttt aaagagtgcc cttcgcgcat cgttaatttt gaattcttct attgcctgct
     1201 gagtctctag aagtgcataa aagccaactc cgcagaatag agtaagaagt tttttgacct
     1261 cctcttgaga cataccctca atgatataaa taagcggtgc ttctattatt gcctcaagtt
     1321 cattttcttt acttcctata atacgagcta gctctctcac tttttcatcg tccgctttac
     1381 tgagcaggtc ttgtaaagcc ttttgaacgt attctagtgc aaagctatct cggatttcat
     1441 ccaacgaaag tccttttgat tttagggctt ttatttctgc aagtttgtct agagtaaatt
     1501 ctggataata gagctttgtt gatttcactt taccctcttt acttttcttt cttgttggtt
     1561 ttgggatgaa cccaagcctt gtgtagaatc taatagtttt tgctgggttt actcctagat
     1621 taatccccct tttctttgat tctttaataa tttcttctat tgtcattcca tccatacaat
     1681 gatattacgc ctttctttta gtatttgtca atcatgtttg ttcatctttt gtatttaata
     1741 aatgttgttt agtttgaata tgttataatt gaaatcactt gatgttcttg ggaacaacat
     1801 tgggtgagaa gtttcaacgt ttaatggctt tccaatagca ataatcaaat aagagtttcc
     1861 actcaaattg ctgaatttgg cgacttagca attcacaagg tgaacgcccc ttaatgcaag
     1921 cttcgcaagg atcaataaag gctataggat tccattttga ttgttttgaa aggaattcag
     1981 ttgatttacg agcatttgaa ttggaggcga gttgaacagc tccattccga agaggttcga
     2041 aaatagcatg ctctccaaca ttatggaacc agtacttgct gttataaaaa tcgggttcac
     2101 gcctgtgcat gataccatgc cagtaacttc cagtttgagt tcggattgtt tggctaatgt
     2161 tatgaggttc tagtaaaaaa tcgtgacaaa cccagacagc cgcaaggcaa gcatttgcca
     2221 tatttgcatc tacgacttca tgaggcgcaa aggcgttatg tatcgtgagt ttattaagtg
     2281 cacttctagt tttaatgttt ggacagccag gtccgagcgg atttagacgt ttttccttca
     2341 aaagttctga aaaaactcgg ccgtatattt cgggtttaaa atcgaaagct tctttcatta
     2401 aaagcgaaaa cacaaaaatt aaacttggta atcaactacc cttctttcta ggcgaacttc
     2461 atttgcaaaa tcaactatct tcatatgttc agggtgtctt tgataaacct caagatcatc
     2521 catactatca aactctgaat ataaaacaac atcggaggcg gcatcagaaa ttgtgatatt
     2581 aatgccaact tcaatatgct taatttcttt tattttactt tttagagatt ccagatgatt
     2641 ttttaatttc tttgcgtttt cctctttgcc tctaccatct gcgaattctt taagccgcca
     2701 tattacaatg tgtttaatca ttttcctttc tcctgtttaa aattaaaatc tttaacaata
     2761 agtctgacct aagatactaa gaataaaagc ttaaagaaag cgattttgtc ttcttttact
     2821 ttgctgatcc atcaaagccg tacccaacag aagtcttctt ttccactatc tctggtttct
     2881 acatagcatg ctcgcgcccg acatttaaga aatgccgagc gggaacctca tgttgaagca
     2941 gggctgcaca gattagtact gcaactatta gtattgcttc tatccatacc ttgtgcatga
     3001 attgacgagc gagctgagtc ccatcaagct tacgtttaac ggaagtaacg tatccagcaa
     3061 ggaacaggtt ataaataacc ctccttttga tcaagggatc gtctgcccac tgttgcagta
     3121 gcggaacact aatgtagcgg tttgaagcgc cgagaaagat gagtgtaagt aaaagcagca
     3181 gcttagcgat cagcgtctga ccgtaaggcg tttgccaaag ggcctgaaaa ctacccactt
     3241 caaaccaggc attatgcact ccggtcagaa atattccagc caacgcgact ccagcaaggg
     3301 tagaaaaccg gccagcgata ttagcaaaca atatcaaact attattggct tgtttgatta
     3361 ctatcgggag aacaatagta gtaaggatta agagtccgcc tccccaaaat gaggtcgcta
     3421 taaaatggag ctagtccatt agctcgggaa gccttaggtt cccctgcgtc agaggcgtgg
     3481 ctcgatgcgc tgcgtgtgaa ggcaatgatg gctccgaagc ccaacatgaa agcgggaatg
     3541 gcttgggaat caagtcgttg cctgcctaat agccaaccga tccataacac tatcatggct
     3601 acggccctta atagccacat ctgcccataa tgggttttga acagaacctt aggtaaacac
     3661 tggaaggatt gctacaacgg aaaggctact catctccgct gctcgtgtag ccagcaataa
     3721 ggtaccgctt aaaataagta acattataca gacagcaagc aactgccata gcctggtgag
     3781 aagcgcctca gaatccaggg caatcatgct cttcgtatca ggagggagca cccataggcg
     3841 gcagcccaga actccgatac atgtggtcag ggctagtagg tctaatacag ttgggatgat
     3901 atggatcagc ttgtcccaca taatcagaat ctcactattt gatggtgaaa atgtaatctc
     3961 cctcagtccg atgtccgtcc cgagcaacgg cgctccagaa aacgcgatag gtgccaggag
     4021 gaagaggagg gaggctaact tcaagtagtg ttggatcgaa aggggctccg tgtccatctc
     4081 ctctatctac ctgttcgcca cttgaatttt gaacgtgaaa agtgctaaag gcaggttcta
     4141 gagcaccatt gaaccagatg cgaacacggg taggagcaac ggtgaccgtt gctcctacct
     4201 ccggctcggc atgttcaggg aagacatggc cccatgctgc atctgtccat aggcagagga
     4261 gcatgaggcc acagagtgtt atgactctgg aaattgttct catcattttc atgttgctta
     4321 acggagaatc ggcctcccga agctatttgg gaacagatcg tcaataaaga aatgtacgat
     4381 agtgagaact ccgacgtttt cacccgaccg atcgttgatt gggatttgaa cctcggcccc
     4441 aagttggatg tacttgccga accagataat tcctgggtta ataaagcctg tggtctcacc
     4501 gtcgcagcca cgatcgaggc atgtttcaaa aggaaattcc accagtacaa tcatacggtt
     4561 gaacggggtg ctaagaccca catctttaac aaaggattga agatactgaa gattgtactg
     4621 aatggcgaac ccataactca gcgtgttcgg gtttcgttct acctcgcgct caatctctcc
     4681 tgtctcttca tccacttctg tggtcacagt tttggtatcc gtgggaaaat taggaccaat
     4741 aactccggta attgccagcg gtctcagata ttttagtgac tccggcaaat ctcctaatcc
     4801 cttgccgaag aagagggcag gcgaaattgt cgtgaatggc tctgcctcaa cctcgcggtt
     4861 tccggtatct tcaatttcga cattgacgcc aatagatagg actgattcat gtggggcatt
     4921 tgttaaaaat tgatattttg ctcctgcctc aaggttaccg aaaccgacct cggtatcctc
     4981 gccatcgggg tctaagatgc ggaactcgtc accaatcgta attccaaagt gtggtgtaat
     5041 ccgcttggtg tactcacctt ctaactcata ggaattaaca ggcggctcat ctccttctcc
     5101 ggattctttt agatagctaa agataaatga tagttcgtca gagacaaagg gatcttcgat
     5161 ggaaagcgtg gtggggaaaa atcgcttgcc tgcaaaccca tgggcccatg cgctcaccgg
     5221 cattaatagt aaaacgacca tacaaataac aactggacaa aacgtcatag ccaataataa
     5281 ctttttcaca tttctcctcc tttataaaaa tcttctattt caagactgtt tctctcacag
     5341 cacattctgt cggatataac tcgacagttt gtgtgaagac ttacatccag ggatgattta
     5401 aatactcatg gatccttttg aactaaacta taaggcttgg aggagagcgt acggagaggt
     5461 ttgtataaaa ctcttttaag taaggatttt catctaatgg aaacaggcaa attatgtatt
     5521 catctaagga taggtttgtt agaggtttaa aaattacaaa aggcaacctc tgattacagc
     5581 agggaaaaca aagacaaagc ttttcatttc cctcctcacc ttctgagttg cttatgtgga
     5641 gtattatgta taataaaagg ttttgatttg agtgagatgg acaatttaag attgagtatg
     5701 atacagagtt ttgaaacgat ggtggtatgt agtgctgaaa cgaaaaaaat ataaagctga
     5761 tagagagaaa tattgtaaga acagaggtta atatatatcg taaaagattt aatttataca
     5821 ttaaaatacg tttgaaatat aggtttggat tcatattttt gattttgttt caactaaaat
     5881 atcattgcaa tatataagat aacttccttc cgttttacta gaaaaagtgc tttctgccca
     5941 tttgttagtg aactaagtat atttttataa tcttctgagg ttgtaattat cttcctattt
     6001 atctcgagta tcacatcgcc aattttaaac ccagccttgt cagcagagct tccaggatcc
     6061 actttagata atataacgcc gctttcgttt tcaaccccta atctttttgc aatttcagga
     6121 gttaattcgt caacagtgag ccccaatcca ctttgtacgt tctgaagttc ctctgaatct
     6181 gaattttctg atgcaagcac tccgagcttt actgacaagg ttttctcagt tccatctctt
     6241 aataacttga gttccacttc cgtacctggg ctgattgcag agactgttcg cgaaagctct
     6301 ccgttttcat taatcttatg tccattaaac tcaaagataa aatctcctct ttttacacct
     6361 gctttgtcag caggacttcc acttgttaca tctcctacga gagctcccat tgcttcaggt
     6421 agtttcattg cctttgctat ctcaggagtt acatcttgaa tagttacacc aagccatcca
     6481 cttacaactg ttcgatctcc ttttagttgt tctatagtat cttttgcgaa attaataggg
     6541 attgcaaagc ctatcccttg agccctcaga aatattatac tagttactcc tactacttcc
     6601 ccttcaagat taaaaagtgg gcccccgctg tttccaggat taatcggagc gtctgtttgt
     6661 atgaagtcat catagtcgcc aaagccgagt attcttccct ttgcacttac tattccagcg
     6721 gtaactgtgt gaccgagacc aaatgggttt cctatggcaa gaacccagtt tccaatttca
     6781 agcttatcag aattccctaa tatgactgga gaaagttttt cttgaggtac tattttgagt
     6841 aaagccaagt cagttttagg atctcttcca acaattttgg cactgtactt ttcttcatct
     6901 tctaaaatca cctgaatctc ttttgctttt tcaacaacat ggttgtttgt aattatgtat
     6961 ccgtcttcgc taataataaa tccagagcct agttctctct gtctaaattc cctttgaggg
     7021 atatctccaa aaaaccttct gaatagctct tgcaaggaat cctcttcttt accatgcggt
     7081 gagggaaatc ggaaggctct tccattacgt atcaagctag ttgtactgat atttactaca
     7141 gaaggcttaa gctttcttac tagcgcggtg aagttagg
//
LOCUS       DATDRL010000117         4159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3731757, whole genome shotgun sequence.
ACCESSION   DATDRL010000117 DATDRL010000000
VERSION     DATDRL010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3731757"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1910)
                     /locus_tag="VI935_04000"
     CDS             complement(<1..1910)
                     /locus_tag="VI935_04000"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HLE24797.1"
                     /translation="MGSNYPKEIIEQLNFGQGKTFYGDATLVILKAFLQSGVSYLGGY
                     PGSPTAGLIDAISDAYEPVLKDMGIYFDCSGNEASAAALLSASISYPIRGSVNWKIVG
                     TNVASDALSHISSSGVTGGAMVIVGEDYGCDSTCVAERALPFALKSTMAVIDPRGDLQ
                     HLAKMIEEGFNLSEYTHMPVMFLLSTRVAHIRGKITCKDNIQPKISMLSPLNDLTTDL
                     NTIPLPPFTYEQERNKFSERIPAARRFILERRLNEIIEGNDQNLGIITHGTIFNSMMR
                     CLYNLGEANLYGESRIPILNLNVIHPLVPEEIVNFLQGKQAVLIVEEGMPNLIEEQIR
                     AIAQRAKLGVDIFGKDILPQAGEYTPDHIIKGIGSFLLKNTTSTYERERIEEKSEYIA
                     KHKEKAANFLKEPVTKRNPVFCTGCPERPVFTAIKILEDKYGKSYYASDIGCYSMSGL
                     PPFDLSNSITGMGIGLASAGALSRMANKRVISFMGDGTFWHSGLTTSITNAVYNKQDA
                     VLIILENFWTSMTGHHENPASGRNMRQEGVGMDIEKALKGAGVNWIKKVNPYDLHESM
                     GALEKAYKADKGLKVIISKAECQLEKTRREKVVIKRKIESGKRMVQPILGIDPDICTG
                     DHSCIRYNGCPSL"
     gene            complement(2056..2511)
                     /locus_tag="VI935_04005"
     CDS             complement(2056..2511)
                     /locus_tag="VI935_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HLE24798.1"
                     /translation="MPERFSWIIPGKLAGMERPGLASGLEDDLKFLKDAGIDIIVNLK
                     EHLRNWDGFEVKHIPINDFKAPKLGDIGGFVKFIDSKIQEGKRIVVHCYAGVGRTNIM
                     LASYLVYLGMNPDQALDLVKEKRPYQSINEEQEEALRKYSYIANDRKKI"
     gene            complement(2654..3445)
                     /locus_tag="VI935_04010"
     CDS             complement(2654..3445)
                     /locus_tag="VI935_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HLE24799.1"
                     /translation="MDSTEKESVTNLYYDIPPSREEAEAIQKELQKRVITQNGFKEIK
                     RVAGIDLAPIQGEERLVCGIIVFSFPELEELERVSARVHEQFPYIPGLLAFREGPAII
                     EAFKKVKYKPDLIMVDGQGIAHPRGFGIASHVGVILDIPSIGVAKERLYGKYNEPPDS
                     EGSWTPLINPNRGSLKSLPPDGNVGSGGGFYQAPYSYPKSQANTIGAVVRTKKGTKPV
                     FVSIGHKIDLRTAVIITRECTKAYRIPEPTRKADIFVAQLKKVKY"
     gene            complement(3432..3683)
                     /locus_tag="VI935_04015"
     CDS             complement(3432..3683)
                     /locus_tag="VI935_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24800.1"
                     /translation="MNEKQPTPQEIAEENKKIRMLRFMVDFTISLIYQANLTKEEAVE
                     HFLKVRDFALRLFPGKEEAFEIIYAPRFKRIITEIYGLH"
     gene            complement(3689..3922)
                     /locus_tag="VI935_04020"
     CDS             complement(3689..3922)
                     /locus_tag="VI935_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24801.1"
                     /translation="MSSQDGEELWNNVPRSSSIKFVRLYSTSAITVATDNPSSLESGV
                     TYRSENTTSDVEYAWITYIITLRPYTTYNSLMI"
BASE COUNT         1088 a          907 c          778 g         1386 t
ORIGIN      
        1 agtgaagggc atccattgta tcttatacac gagtggtctc ctgtgcaaat atcaggatca
       61 atgcctagta ttggctgaac catacgctta ccagattcga tttttctttt tattacgacc
      121 ttttctcttc gggttttttc aagctggcat tcagctttcg aaattattac cttcaatccc
      181 ttatctgctt tgtaagcctt ctcaagagcc cccattgatt catgtagatc atagggatta
      241 acctttttga tccaattaac accggctccc ttgagtgcct tttcaatatc cataccgacg
      301 ccttcctgcc tcatgttcct tcctgaagca gggttttcgt gatgaccagt catggatgtc
      361 cagaaattct caaggataat taggaccgca tcctgtttgt tatatacggc atttgtaata
      421 ctagtcgtaa gtccgctatg ccaaaaggtt ccatctccca taaatgagat tactctctta
      481 ttagccattc gggaaagggc tcctgctgac gcaagtccaa tccccattcc tgttatagaa
      541 ttgctgaggt caaaaggtgg gagtccactc attgaatagc acccaatatc cgaagcatag
      601 taagatttac cgtatttatc ttcaaggatc tttattgcgg taaagactgg tctctcaggg
      661 caaccagtgc aaaaaacggg gtttctcttt gttacaggct ccttaagaaa gtttgccgct
      721 ttttctttgt gcttggctat gtattcgctt ttttcttcaa ttctctctct ttcataagta
      781 gaggttgtat tcttaagaag gaaactgccg atacccttaa ttatatggtc tggtgtatat
      841 tcaccagctt gagggagaat gtccttacca aatatatcaa cacctaattt cgccctctga
      901 gcgattgctc ttatctgttc ttcgattaga ttaggcatcc cttcttcaac aattagtact
      961 gcctgtttac cctgcaggaa attaactatt tcttctggaa caagtggatg aatcacgttt
     1021 agattgagta tcggaattct actttcacca taaagattag cttcacctag gttatataga
     1081 cacctcatca tggagttgaa tatcgttcca tgggtgatta ttcctaagtt ctgatcatta
     1141 ccctcgataa tttcgttaag ccttctttct agaatgaacc gtcttgctgc aggtattctt
     1201 tctgaaaact tatttctttc ctgctcatac gtaaatggtg gaaggggtat tgtatttaag
     1261 tcggtagtta agtcattaag ggggctcagc atacttatct ttggctggat attatcttta
     1321 caggttattt ttcctcttat gtgtgcgacc cttgtagata aaagaaacat caccggcatg
     1381 tgtgtatatt cagagagatt aaatccctct tcaatcattt ttgctaaatg ctgcaggtct
     1441 cctcttgggt caatcacagc catcgtggac tttagcgcaa agggaagcgc cctttctgca
     1501 acgcatgtac tgtcacaacc atagtcttct ccaacgataa ccatagctcc accagtaacc
     1561 cctgaagaag agatatgcga aagggcatct gatgcaacgt tagttcctac gattttccag
     1621 ttcactgaac cacgtattgg atagctaata gacgctgaaa gaagcgctgc tgccgaggcc
     1681 tcattacctg agcagtcaaa gtatatcccc atgtctttta atacaggttc gtaggcatcg
     1741 gatatagcgt caattagacc cgctgtaggc gatcctggat acccacccaa gtagcttaca
     1801 cctgattgaa gaaaggcttt taggattact agtgttgcat caccgtaaaa cgtttttcct
     1861 tggccaaaat ttaattgctc tattatttct ttaggataat tactacccat ttccctcctg
     1921 cataaatgca atcaattaca tttataaatt ttgttgtatt aactgtacca gaaaaagact
     1981 agagatgcta gagattctta gtctttaaga tagtgggagt cactaactgg tttaaattat
     2041 tcacaaaaat ttgcattaaa tcttttttcg atcgtttgct atgtaggagt attttctcag
     2101 cgcttcctcc tgctcttcat ttattgattg ataaggtctc ttctctttaa ctaaatctag
     2161 tgcctggtca gggttcattc ccaaatatac aaggtagctt gcaagcataa tatttgttct
     2221 tcccacacca gcatagcagt gaactacaat ccgttttcct tcttggattt tactatcaat
     2281 aaattttacg aaccctccaa tgtcacccag ttttggggcc ttaaaatcat ttattggaat
     2341 atgctttact tcgaatccat cccaatttct caagtgttct ttaaggttga caattatgtc
     2401 aatcccagca tcttttagga attttaagtc atcttccagt ccggaggcta aacctggcct
     2461 ttccattccc gcaagcttcc caggtattat ccaagaaaat cgctcaggca ttgttcgatc
     2521 cttaatcgtt aggcgtaagt cgtttggtgt taggccatta ctttacctct tacgcctcac
     2581 gctttacgaa taaaactttg caccctttcg actggccccg acacagacta tacaccttaa
     2641 ggtggcacag ttcttagtat tttacttttt ttaactgtgc gacaaaaata tcggcctttc
     2701 ttgtaggttc agggattcta taagccttag tgcattcacg tgtgattatt acagcggttc
     2761 tcaaatctat tttatgcccg attgatacaa aaacgggctt cgttcctttt ttggttctaa
     2821 ctacagcacc gattgtgttt gcttgggatt tggggtagct ataaggagcc tgataaaacc
     2881 cccctccaga ccccacattc ccatccggag gtagggattt cagggagcct ctattcggat
     2941 ttattaaagg agtccatgag ccttcactgt caggcggttc gttgtattta ccatagagcc
     3001 tttcctttgc tacaccgatt gatggaatat ctagaatcac cccaacatga cttgcgattc
     3061 cgaacccacg tggatgggct atcccctgtc cgtcaaccat aatcaggtca ggtttatatt
     3121 tgaccttttt aaaggcttcg attatcgctg gcccttctct aaaagctaag agtcctggga
     3181 tgtaagggaa ctgctcatga acccttgccg aaaccctttc tagttcttcg agttctggga
     3241 atgaaaaaac aattatgccg catacaagcc tttcttcacc ctggataggc gcaaggtcta
     3301 tccctgctac tctttttatc tctttaaagc cattttgcgt aattaccctt ttttgaagct
     3361 ctttctgaat cgcttcagct tcttcacgag aaggtggaat atcataatag aggttggtta
     3421 cgctttcttt ttcagtggag tccataaatt tcagtaatga ttctcttgaa tctaggggcg
     3481 tagattatct caaaggcctc ttcctttccg ggaaagagtc ttaaagcaaa atctctcact
     3541 tttagaaagt gctctaccgc ttcttctttt gtaaggtttg cctgatagat aagggatatt
     3601 gtaaaatcaa ccataaacct aagcattctt atcttcttgt tttcttccgc tatttcttga
     3661 ggggtgggtt gtttctcgtt catacctatt aaatcataag cgagttgtat gttgtataag
     3721 gtcttagagt aattatatac gttatccagg catattctac atctgaagtt gtattttcgg
     3781 aacggtatgt aacacctgat tcaagtgaac taggattatc ggtggcaact gtaatagcgc
     3841 ttgtgctata tagcctaacg aatttgatcg aggagcttct tgggacgttg ttccataatt
     3901 cttctccatc ttgacttgac aattgatagt catcccgtta aagtgaaaag taatctaagt
     3961 ccgtattaaa ataaccttac tagtttggat tatctataat tgctagtaat ttttcaaaaa
     4021 tcaataaccc tgcagcaagc tgcagggtat ggtcttaaaa atataccgtt atagctgtca
     4081 ttgcgaggag aaaagcgacg aagcaatcta aaataagaga ttgcttcact tcgttcgcaa
     4141 tgacaataca ctattgtgg
//
LOCUS       DATDRL010000118         7458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_827637, whole genome shotgun sequence.
ACCESSION   DATDRL010000118 DATDRL010000000
VERSION     DATDRL010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827637"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VI935_04025"
     CDS             <1..194
                     /locus_tag="VI935_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent aldehyde dehydrogenase"
                     /protein_id="HLE24802.1"
                     /translation="HNTVMFDKPQKSVIYGPFRIWPKPPWFVTHKTANKLAPILTQFE
                     ARHCCWSLLPSIIWHALQG"
     gene            581..1405
                     /locus_tag="VI935_04030"
     CDS             581..1405
                     /locus_tag="VI935_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HLE24803.1"
                     /translation="MNNKEVNFKEGLQFWVKLGFINFGGPAGQIAIMHKELVDRKRWI
                     SESQFLRALNFCMILPGPEAQQLATYIGWRLHGTLGGIVAGSFFVIPSIFVLLLLSWL
                     VAAHADVPAIAGLLYGVQPVVIAIVLEAVLRIGKRTLRHAILVVFAIVAFVAIRFLSI
                     SFPLVIAAGALSGILLGRWWPSVFQPLRHAPLTPSGTETLIHQNIPTTYPSMSRNLKL
                     VGIFFVLWFIPIGAIWFWRGGSDVLIKEALFFTKAAFVTFGGVYAVLSYIADVSVN"
     gene            1415..1882
                     /locus_tag="VI935_04035"
     CDS             1415..1882
                     /locus_tag="VI935_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HLE24804.1"
                     /translation="MEASQMVQGLALAESTPGPLIMVTQYVRFLGAWNFHGEYNALYY
                     GVIGALVTIYVTFLPCFMFIFVGAPYIEALAGNRDIQSALVGVTSAVVGVIVNLAVFF
                     GSKVLFPASGKFDGFALAISIASFILLQRFRIPIHLLVSVGAVIGMIWRLLHL"
     gene            1904..2341
                     /locus_tag="VI935_04040"
     CDS             1904..2341
                     /locus_tag="VI935_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014011666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HLE24805.1"
                     /translation="MKWVTRERVKVDRVACPWLIKKFVDPEAEFLFVPKESVLEVAER
                     EKAIPFDIEGVELGHHEGKCSFEAIVAKYRIEDPAIQLLANIVHGADVTQDLHGRPEA
                     AGLKAVAEGFQYLGLKDDHEILAKEFIVYDALYAYCQQAVFDA"
     gene            2604..3986
                     /gene="argH"
                     /locus_tag="VI935_04045"
     CDS             2604..3986
                     /gene="argH"
                     /locus_tag="VI935_04045"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HLE24806.1"
                     /translation="MPKKPWGGRFKKVTHKLVDKYTSSIDFDKRLYGEDIEGSIAHAK
                     MLGKQGIISKQESQQIVKALEEIRGEIEKGKFPYREKLEDIHLNIEKRLIEKIGEIGG
                     KLHTARSRNDQVVLDERLYLRREVGEILSLIKDLARAFLDLAEKNIDVIMPAYTHLQR
                     AQPILLSHHLLAYFEMLKRDRERYIDCLKRVNIMTLGAGAVAGTSFPIDRDYVADLLN
                     FPQVTRNSVDTVSDRDFILEFISTSAILIMHLSRLSEELILWSSKEFDFVDLGDEFTT
                     GSSIMPQKRNPDVPELIRGKTGRVYGNLMALLAIMKGLPLSYNRDLQEDKEPMFDTAD
                     TVKSSLHVLSEMMKHIKYKSENMRKALSKGFATATDLSDYLTRKGTPFRKAHEVTGKI
                     VRYAEEKERELIQLNISELKMFSSLFEGDVFDHITIEGSISSKKSYGGTAKENVLKMI
                     QECQKELAKW"
     gene            4034..4357
                     /locus_tag="VI935_04050"
     CDS             4034..4357
                     /locus_tag="VI935_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HLE24807.1"
                     /translation="MNKDELKQRTKQYALQIIKLVESLPKGLTAEVLSRQLLRSGTSF
                     GANCRLRNTDFRISFPKSAIRIPKLQVIHRVLLHISRLFQTQAWMKSPYRIFHRIPSS
                     LPLQA"
     gene            complement(4245..5855)
                     /locus_tag="VI935_04055"
     CDS             complement(4245..5855)
                     /locus_tag="VI935_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLE24808.1"
                     /translation="MTEDNRELGLITGGSLTEGLQMKLSPDRSVEEVKAGKFVVVFGL
                     YHKFFSLITDVRLDASSTNVLINPPSREDETLRQVLSGTSTISTVELRPMLMITHDDN
                     EPKPVKTIPPHFALVSEAAKGDVSLVFGTEDKGGRFYNIGSPLDMDIPVCMNLDRFVE
                     RSNGIFGKSGTGKTFLTRVMLAGIIKHGKAVNLIFDMHNEYGWEATKEGVGEFVKGLK
                     QLFPEKVAIFTLDPQSTRRRKAKPDVEVYISLDQIKIEDIALLQDELQLHSTAVESVY
                     TIYREYGDSWLSELLSWNDEELKEMAQKLGAHHGSLSALPRKLKRIKDLPFIVDDDRG
                     EGTIQRVIEYLERGINIVFEFGKRNSLLSYLLVANIISRRIHDRYIEKTEDYLASQNP
                     ADRPRQLMITIEEAHKFLNPQAARQTIFGTIARELRKYYVTMMIVDQRPSGIDDEVIS
                     QIGTRITALLNDEKDIQSVLTGVSNTSGLRSVLASLDTKQQALILGHAVPMPVVVKTR
                     EYDEKFYKEISSMPESGKAGKYVEELYG"
     gene            6468..6543
                     /locus_tag="VI935_04060"
     tRNA            6468..6543
                     /locus_tag="VI935_04060"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6502..6504,aa:Glu,seq:ctc)
     gene            complement(6728..6937)
                     /locus_tag="VI935_04065"
     CDS             complement(6728..6937)
                     /locus_tag="VI935_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24809.1"
                     /translation="MNIKRVILGLIVILLIILGITKIMGMLKESKEVSTTYMKEAIGL
                     KTKAKGLVEQSEKQVKEREKMVEEE"
     gene            7117..7401
                     /locus_tag="VI935_04070"
     CDS             7117..7401
                     /locus_tag="VI935_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2103 domain-containing protein"
                     /protein_id="HLE24810.1"
                     /translation="MRHRKHKIKRQHSMIKGLREFLEENVSTLDFVDGIIPGEIKVGS
                     ATGENLIVKYKYSTVSGAKLIARSGSSIQEVFVVTRFPEKLREVIEVIAE"
BASE COUNT         2159 a         1488 c         1567 g         2244 t
ORIGIN      
        1 tccacaatac ggttatgttc gataaacctc aaaaaagcgt tatatatggg ccatttcgaa
       61 tctggcctaa gcccccttgg tttgttaccc acaaaacagc gaataagctt gcgccaatac
      121 tcactcaatt cgaagctcgc cactgctgtt ggtcgcttct tccctctata atatggcacg
      181 ccctacaagg ataaggacgc cgataactca atcctgccag caagcctggc gggctccccc
      241 tgtatttgag tagacaacga gccccttcga caaagctcag ggcatgcttg ctcgttgtct
      301 taaatgccca gcattataat gccacatttc aataggaaat tggtattact tcatcttttc
      361 gtctctgtag cagcagcggg atctgggggt atctccgatc cagtcgggga tcaagaggga
      421 gggtatttta ggggaaacct tttccagctg ctacaaagtc gcgccaggac tgtcctgaac
      481 actgtcgaag ggtagtactc ttataacgaa ctctaaaagt ttattttatt tgtgtaaatt
      541 cgtgtttatt tgtggctaaa tagttacatg gtagaaataa ttgaataaca aggaagttaa
      601 tttcaaagag gggctgcagt tctgggtgaa gttgggtttt attaactttg gtggccctgc
      661 tggacagatc gcaatcatgc acaaggaact ggtagacagg aagcgctgga tttctgaaag
      721 ccaatttctt agagccctaa acttctgtat gattcttcca ggcccagagg ctcaacagct
      781 tgctacgtat atcggatggc gtcttcatgg aaccttaggc ggtattgttg caggctcttt
      841 ctttgtaata ccatccatat ttgtcttgct cttattgagt tggctagtcg ctgcacacgc
      901 tgatgtgcct gcgattgccg gtctgcttta cggtgttcaa cctgttgtaa tcgctatagt
      961 acttgaagcc gttcttcgga ttgggaaacg aactctacgc catgcgattt tagtagtttt
     1021 tgcaattgta gcctttgtcg ctatccgttt tctatccatt tcattccctt tggttattgc
     1081 cgctggtgcc ttaagcggaa ttcttcttgg acgatggtgg cctagtgtat ttcaaccgct
     1141 taggcatgct ccccttacac ccagtgggac ggaaactctt atccatcaaa atattccaac
     1201 aacctaccct tctatgagtc gcaatctaaa gctcgtaggc atattttttg tcctctggtt
     1261 tatacctatt ggtgctattt ggttctggcg tggtggctcg gatgtcctaa tcaaagaggc
     1321 cctcttcttc acaaaagctg cgtttgtgac attcggtgga gtctatgcgg ttctcagtta
     1381 catcgcagat gtttcagtca attagtacaa ttggctggaa gcaagccaaa tggtacaagg
     1441 acttgcttta gctgaatcaa cgccgggtcc cctaattatg gtcacccagt atgtgcgttt
     1501 tctaggagcc tggaattttc atggcgaata taatgcgctt tattatggtg ttataggagc
     1561 ccttgtcact atttatgtaa cttttcttcc ttgctttatg ttcatttttg ttggggcccc
     1621 atatatagag gcgttagctg ggaaccgaga tattcaatcc gctttagtag gtgttacatc
     1681 ggctgttgta ggagttatag taaatctggc tgtatttttc gggagtaaag tattgtttcc
     1741 tgccagtggc aaatttgacg gatttgccct ggccatctcg atagcttcgt ttatcctgtt
     1801 acagaggttc cgtattccga tacacctatt ggtatcagta ggtgcagtca ttggaatgat
     1861 atggcgttta ttacacctct aaaagtggag gaggtagcaa gagatgaagt gggttacccg
     1921 tgagcgggtg aaggtagacc gagttgcctg tccttggctc atcaaaaagt ttgttgaccc
     1981 ggaggcagag tttttattcg ttcctaaaga gtcggttcta gaggtagcag aacgtgaaaa
     2041 agccattccc tttgatatag agggagtgga attgggacat catgaaggaa aatgtagttt
     2101 tgaggctata gtggcaaaat atcggattga agacccggca atccaactgc tagctaatat
     2161 agtacacgga gcagatgtca cccaagatct gcacggccga ccggaagccg cagggcttaa
     2221 agccgttgcc gaaggcttcc agtatttggg tcttaaagat gatcatgaga ttctggcaaa
     2281 agaatttatc gtttatgatg ccctctatgc ttactgccag caagcagtat ttgatgcgta
     2341 gctttctctg gttcgcagtg tgaaccagag ggtaataaac agtgcatcaa atggaaataa
     2401 taaggtgatc cttgcaacga aaagtagtcc gggaaatgat atgggttaaa caattttttc
     2461 tggttttccg tctttgcaca tgttaatgta aataacagag ataggcgggg ttttctaacc
     2521 ccgctggact ggacaagaat gtcctgtcta tcgttgtata atttctctct taagatagta
     2581 taaatcgaga agaggattga gacttgccta aaaaaccctg gggagggagg tttaaaaaag
     2641 taacgcacaa gttggttgat aaatatacat cctcgattga ctttgataaa agactctatg
     2701 gagaagatat agaaggaagc atcgcacatg caaagatgct tggtaagcaa ggaatcatat
     2761 caaaacagga atcacaacag atagtaaaag cgcttgagga aattaggggg gagatcgaaa
     2821 agggaaagtt cccctacaga gagaagcttg aggatataca ccttaatatc gaaaaacgac
     2881 tcatagaaaa aattggcgaa attggcggga agctccacac ggcaagaagt agaaatgacc
     2941 aagtggtgct tgatgaacgt ttgtatctaa gacgagaagt cggtgaaatt ctttctttga
     3001 taaaagacct tgcaagagct ttcttagatt tagcagagaa aaatatagat gtaattatgc
     3061 ccgcatatac gcatcttcaa agggcgcaac ctattctttt atcacaccat ctacttgcct
     3121 actttgagat gctaaaacga gatagagagc gttatattga ttgtctaaaa agagtaaata
     3181 ttatgacact tggcgctgga gcagttgcag gtacttcatt tcccatagac agggattatg
     3241 ttgcggatct tcttaatttt cctcaagtta caagaaatag cgttgacaca gtaagtgata
     3301 gagatttcat cttagaattc atttcaactt cagccattct aattatgcat ttaagcagac
     3361 tttcggagga acttatctta tggtctagca aagaatttga tttcgttgac cttggagatg
     3421 agtttacaac aggctcgagc ataatgcctc aaaagagaaa ccccgatgtt cctgaactta
     3481 tacgtgggaa aacaggaagg gtctatggaa acctgatggc gctccttgcc atcatgaaag
     3541 ggctacctct ctcttacaac agagaccttc aggaagataa agaacccatg tttgacacag
     3601 ccgataccgt aaaatcctct cttcacgtgc tatcagaaat gatgaaacac ataaaataca
     3661 aatccgagaa tatgagaaaa gcactttcca agggcttcgc tactgcaact gacctatctg
     3721 actatttgac tcgaaaggga acaccgttta gaaaggcaca cgaagttacg ggaaagatag
     3781 ttagatacgc agaggaaaag gaaagagaac taatacagct aaacatttca gaattaaaga
     3841 tgttctcaag cctatttgag ggggatgtat ttgaccatat tactattgaa ggttcgatct
     3901 caagcaaaaa atcctacggt ggaactgcaa aagaaaatgt tttaaaaatg atccaagaat
     3961 gccaaaagga gctagcaaag tggtaaccta cctcaaatgc tgaattcgga tggcggaacg
     4021 cggggtgcaa gagatgaata aagacgagtt gaagcagaga acgaagcaat atgcacttca
     4081 gatcatcaaa ttggtcgaat cattacccaa aggcctaact gccgaagtgc tgagtagaca
     4141 attgttgcgc tcaggcacgt catttggagc gaattgtaga ttgcggaata cagatttcag
     4201 aatatccttt ccgaaatctg caatccgaat tccgaaatta caggtcatcc atagagttct
     4261 tctacatatt tcccggcttt tccagactca ggcatggatg aaatctcctt atagaatttt
     4321 tcatcgtatt ccctcgtctt taccactaca ggcataggca ctgcatgacc gagtataagt
     4381 gcctgttgct ttgtgtcaag gctagcaaga actgatctaa gacccgatgt attactcaca
     4441 cctgtaagaa ccgactgaat atccttttca tcgttaagta gggctgttat cctagtccct
     4501 atttgtgaaa tcacctcgtc atctatacca gatggtctct gatctacaat catcatagtc
     4561 acatagtatt ttcttagctc acgtgctatc gtaccgaaaa tagtctgcct cgccgcctgt
     4621 gggtttaaaa acttatgcgc ttcttcaata gtaatcataa gctgtcgagg acggtcagca
     4681 gggttctgac tagcaagata atcctctgtt ttctcaatat aacggtcgtg tatccttctt
     4741 gatataatat tcgccacaag aaggtatgaa agcagactgt ttctctttcc gaattcaaac
     4801 actatgttta taccgcgctc aaggtattct atgactcttt gaatagttcc ctctccgcga
     4861 tcgtcatcca caatgaaagg aaggtcttta atccttttga gttttcttgg aagagcagat
     4921 aatgaaccat gatgtgcacc taatttttgt gccatttctt ttaattcttc gtcattccaa
     4981 gaaaggagtt cgctcaacca gctatcgcca tattctctat agatggtata tacagactcg
     5041 actgctgtac tatgtagttg aagttcatct tgaagaagcg caatgtcttc gatttttatc
     5101 tgatctaggg aaatatatac ctctacatct ggttttgctt tgcgacgcct tgtagattga
     5161 gggtctaagg taaatatcgc aactttttca ggaaaaagtt gcttgagtcc ctttacgaac
     5221 tcgcccactc cttcctttgt agcttcccaa ccatattcgt tgtgcatatc aaaaattaag
     5281 tttacagctt ttccatgttt gataatacca gctagcatca ctctggtgag aaatgtctta
     5341 ccggttccgc tttttccaaa aatcccatta cttctctcaa caaatctatc aaggttcatg
     5401 cataccggga tatccatatc aagcggagag ccgatattat aaaaccttcc gcccttatct
     5461 tcggttccaa ataccaagct cacatccccc tttgctgctt ctgagacaag tgcgaaatgg
     5521 ggtggtattg tctttacagg ttttggttcg ttgtcgtcat gagttatcat taacatgggt
     5581 cttagttcca cagtcgaaat ggtgctcgtt ccggaaagaa cctgtcttaa tgtctcatct
     5641 tccctcgatg gtggatttat aagcacatta gttgacgaag cgtcaagcct aacatcagta
     5701 ataagagaga agaacttatg ataaagccca aaaacaacta cgaatttacc agccttaact
     5761 tcctcaacgc ttctatcagg gcttagcttc atctgaagcc cttcagttaa tgagccacca
     5821 gttattagac caagttccct gttatcttca gtcatttttt tatattgaga atagcaaagt
     5881 aagacccaac atgcaagcag tcagtactaa tacttaactc tgagtcaaaa gcttccttag
     5941 tttcatctaa agaaatgtga acaaaaactt atattgcaag taattgagac atatgaatta
     6001 atctccgcat gaagtaatta taaatttggc attagagaat gtgggaataa tgtagtctat
     6061 gaattttgga aggtaggatt atgggcggga taatagagca tttctggcat tgctaaatac
     6121 caacgttatt tcttacgctt ggaatatata tgtccatttt cgggttatac agcggaatgg
     6181 aaaaggtgca ttagcagttg gaagatcccg gcagagcgtt agcggttgct tagtgctcac
     6241 gaatagggat cattggtttg agtctagttg ttgtcggcat aggttggctg ttggattttg
     6301 atgcgctctc ctgttatcac ttatcatctg ttgtgaaaag cttcttgaaa cctcgattgt
     6361 cattgccact ctcaaaagat cactacggca taactcttaa caaaacctag tttgtatttt
     6421 attgatttca atcgctaatg tgctaaatta ccatgcagaa aattagtggc cccttcgtct
     6481 agaggtccag gacgttgccc tctcaaggca gaaacacggg ttcgagtccc gtaggggccg
     6541 ccataaaaac tctctctaaa tttaagctaa tttcatttta tcaaaagatg gtgaagacag
     6601 tttagataca ggtatgtttg atgtaagttg cagatgctta tataagattt aaatgctctt
     6661 tttttgtttt ttacaacttt aaagtttctt cgatactttc tagcaaaaaa aatgtcaaat
     6721 gctgctttta ttcttcctct accatctttt ctctctcttt aacctgcttc tcgctttgtt
     6781 ccactaaacc ctttgctttt gttttgagac ctattgcttc cttcatatat gtagttgaca
     6841 cttcttttga ttccttcagc attcccatta ttttagttat acctaatatt attaacaaaa
     6901 taactattaa acctaatatc acccgtttta tattcataat attattataa aaccattgta
     6961 agataaattc tacagattat attctgcact gaagatatat aaccgaagaa acaaagattg
     7021 ttcaattaaa aaaatattac aaaggtatta aaccgagtaa aatttataga gcctttatat
     7081 caaatacgac tttgaactac acgttcattc gccaagatga gacatagaaa gcataaaata
     7141 aagcgtcagc atagcatgat aaaggggtta agagaattcc tagaagaaaa tgtttctacc
     7201 cttgacttcg tagatgggat tatccctggt gagataaaag tgggaagtgc tactggagag
     7261 aacctaattg tgaaatataa gtacagtact gttagtggcg caaagttaat tgcaagaagc
     7321 ggttcttcta ttcaagaggt gtttgttgta acaaggtttc cagaaaagct tagagaggta
     7381 attgaagtaa ttgcagagta gaaacgcatt gcgatgcatc tctaccttaa ccaaacttta
     7441 agtgaccgct tcccaaga
//
LOCUS       DATDRL010000119         6507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1886203, whole genome shotgun sequence.
ACCESSION   DATDRL010000119 DATDRL010000000
VERSION     DATDRL010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1886203"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VI935_04075"
     CDS             <1..634
                     /locus_tag="VI935_04075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019509423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLE24811.1"
                     /translation="ARDGAGAKRHLEDSLRRLKTDHLDLWQVHAVESPEDVDGRIQHG
                     VFEAMVEAKESGKARHIGFTGHTRPAAHQRVLEKTDIFDTCQMPINLADPSYESFIEG
                     VLPKLVERKIGVLAMKSLANGGFFGGSQHGEHGDNPKLVPNRVSIAEAIHFVWSLPVS
                     VLITGPDNAEQMKEKIALARSFVKMDEKQRQVLVKKVADLAGRRVEFYKA"
     gene            631..804
                     /locus_tag="VI935_04080"
     CDS             631..804
                     /locus_tag="VI935_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24812.1"
                     /translation="MKRKRESERKERVSPSPGRQAGSRKTPNSLRGIETLEDLRHALL
                     LANLEKTLVLVWY"
     gene            897..1286
                     /locus_tag="VI935_04085"
     CDS             897..1286
                     /locus_tag="VI935_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HLE24813.1"
                     /translation="MSQRTKDWLQQAERDLEAAKWSLKGKFYEWACFNCQQATEKALK
                     ALYQHLNLDAWGHSVSKLMADLPQKTRPPKSLIGKAIYLDRFYIPTRYPNDLPSGAPM
                     SYFTQKDAKEAIKYGEEIIRYCKDKIS"
     gene            1243..1611
                     /locus_tag="VI935_04090"
     CDS             1243..1611
                     /locus_tag="VI935_04090"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLE24814.1"
                     /translation="MAKKSYDTVRIKSLNKNKVLRKLRRVAGGIIKENPKVMEVSLFG
                     SLAKGNYTGRSDADILIVLKEDSRRFIDRIPPYLAAFLYADVPVDVFPYTEEELKQLD
                     IKKSSFIKRIQDEKIILINR"
     gene            complement(1844..2533)
                     /locus_tag="VI935_04095"
     CDS             complement(1844..2533)
                     /locus_tag="VI935_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HLE24815.1"
                     /translation="MKRFLGLLLIFVISLTQFGGCDSSTEGGKDLEIIYVAIGASDAV
                     GIGAIPFTNGYVFRIRDALEEESFNVELRNLGILAAELDEINEAVDMLLSEIGSGLVG
                     NPDLITIWVGANDIISGVNPEEFESNLESVLVRLFAETTAFIVIANVPDLTQLPRFIE
                     NPSDNVTIERISAFNAIIESLAVEFDIPLVDLFAEPIDDMLVSQDGFHPSNEGHQRIA
                     NLFLQKILPEF"
     gene            complement(2783..2914)
                     /locus_tag="VI935_04100"
     CDS             complement(2783..2914)
                     /locus_tag="VI935_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24816.1"
                     /translation="MNGIPGLSYTEIIKALQVAGFIVIRQQGGEDGRLFLADNFVKK"
     gene            complement(2911..3138)
                     /locus_tag="VI935_04105"
     CDS             complement(2911..3138)
                     /locus_tag="VI935_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLE24817.1"
                     /translation="MELYDFPHLSVGAEHRSAHFFQFPMDVPSLPGCIGEGEMEEEAP
                     ANIKEATELYLEPIEAEMFMTESRIVKEITI"
     gene            3191..3466
                     /locus_tag="VI935_04110"
     CDS             3191..3466
                     /locus_tag="VI935_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24818.1"
                     /translation="MAILNSFAGEFDMSKLLSLKLRDKVFLDAEVILSWSQKPRNAYF
                     NEAINLYNKLWKRKLLKKTLAEESALVADESLLVMEAFELLEDELDE"
     gene            3473..3799
                     /locus_tag="VI935_04115"
     CDS             3473..3799
                     /locus_tag="VI935_04115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HLE24819.1"
                     /translation="MENKKWHVYLANVNPRTFVSLSRAMGTEPGKVRPVEVAQIDLLN
                     GLHPSTVVCPLTTKVQPRARFLRVHHTIGETGLKEQSDIIVDQIRAIYNRRFLGHTGK
                     FLVKAK"
     gene            complement(3854..4579)
                     /locus_tag="VI935_04120"
     CDS             complement(3854..4579)
                     /locus_tag="VI935_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLE24820.1"
                     /translation="MFRKFCDHITFSLISLFKVSFFLSLLFLFAISFVKSVHIHYKES
                     LASLNVLNSPVSRFKERKFFYSEIQETTANPEYLREEIEILRVILRFSSAISTYDAMK
                     LAKLIREESQNCGLDPFLILAIIQVESEFAPRAVSPKGAMGLMQIMPETAEFIAGELG
                     VSIDGIKSLYNPFINVRLGVHYLSMLIDRFKSVEWALHAYNAGPSFLNASGFERGIPA
                     YVRKVLRFKIILESQRIVEEQSL"
     gene            complement(4630..4887)
                     /locus_tag="VI935_04125"
     CDS             complement(4630..4887)
                     /locus_tag="VI935_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24821.1"
                     /translation="MNRHYLRSLRGILSEAFFEPQCGSKGNEKQRRGRSNLSGSVIPN
                     PVLSLSEGAVRNLRFLVLLAMTLETPVASYGELSSSKENKI"
     gene            complement(4985..5167)
                     /locus_tag="VI935_04130"
     CDS             complement(4985..5167)
                     /locus_tag="VI935_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24822.1"
                     /translation="MKAREEDMIMNINEILMEIKRIKKETKKSSWLLGEELTDQIIRV
                     LEEREEQVLEHLMWSA"
     gene            complement(5320..5517)
                     /locus_tag="VI935_04135"
     CDS             complement(5320..5517)
                     /locus_tag="VI935_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24823.1"
                     /translation="MNKRDNNTKVKDFSDGVKGIAGLVREKYQNVLQHTLHKIEENQT
                     VLKNSVKEQLNSNLDYTTSER"
     gene            complement(6258..>6507)
                     /locus_tag="VI935_04140"
     CDS             complement(6258..>6507)
                     /locus_tag="VI935_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HLE24824.1"
                     /translation="LKLDLKVYQIGPYETSNIVLLVCAFLIGVFFAIAWGAFYAVSIR
                     SQIKERDNKIKELQSQREDIQIHGFRETPKEETFNINK"
BASE COUNT         1981 a         1281 c         1278 g         1967 t
ORIGIN      
        1 cgcccgtgac ggcgctggcg ccaagcgcca tcttgaagac tccctacgcc gattgaaaac
       61 cgatcatctc gacttgtggc aggtacacgc cgtagaaagt ccagaagatg tggatggtcg
      121 aatccagcat ggcgtgttcg aggccatggt cgaggcgaag gagtccggta aggcgcgcca
      181 tataggtttt accggccata ctcgccccgc agcccaccag cgcgtgcttg aaaagacgga
      241 catcttcgac acctgtcaaa tgcccataaa tctggctgac ccgagctacg aaagctttat
      301 cgaaggcgtt ctccctaaac tcgtcgagcg caaaattggg gtgctggcga tgaagtcgct
      361 cgccaatgga ggattcttcg gcggttctca gcacggggag cacggtgaca accctaaact
      421 cgtgccaaat cgcgtcagca tcgcggaggc aatccatttt gtctggtcac tgccagtcag
      481 tgttttaatc acaggccccg ataacgccga gcagatgaaa gaaaagatcg cgctggcgcg
      541 gtcattcgtc aagatggacg aaaaacagcg tcaggtattg gtgaaaaagg tcgccgattt
      601 ggctgggcgg cgtgtggagt tttacaaggc atgaaacgca agagggaaag cgagagaaaa
      661 gagagggtgt ctccctcacc agggagacaa gctggaagta gaaaaactcc taattccctt
      721 aggggaattg aaacattgga ggatctacgt catgctctcc tgcttgctaa tttagaaaaa
      781 actctagttc tcgtatggta ttgaagcttt ctgctagttt gagttaaaaa actaagatga
      841 atatgacata gacaatgagg ttagaataaa gtaagttcat taacgcgagg aaataggtgt
      901 ctcaaagaac taaagactgg cttcagcaag cggagaggga tttagaagcc gcaaagtgga
      961 gtctcaaggg taagttctat gaatgggctt gttttaattg tcagcaggcc acagaaaaag
     1021 cactaaaagc gctctatcaa catcttaatt tggatgcctg gggccattca gtgagtaagc
     1081 ttatggctga tttaccccaa aaaactcgtc ctccaaaaag ccttatagga aaggctatct
     1141 acctcgaccg cttttacata cccacgagat atcccaatga cttgccctca ggagcaccta
     1201 tgtcatattt tactcagaag gatgccaagg aggctataaa atatggcgaa gaaatcatac
     1261 gatactgtaa ggataaaatc tcttaataag aataaggttc ttagaaaatt aaggcgtgtt
     1321 gccggaggaa taataaaaga gaacccaaaa gtaatggagg taagcctctt cggttcttta
     1381 gctaagggga attatacagg aaggagcgac gccgatatac tgattgttct aaaggaagat
     1441 tcaaggagat ttatagacag gatccctccc tatttagctg cctttcttta tgccgatgtc
     1501 cctgttgatg tttttccata tacggaggag gaactcaagc aactggatat taaaaaaagt
     1561 tcgttcatta aacggatcca ggatgaaaaa ataattctta taaacaggtg aataaaatag
     1621 ggcggataaa ttagtaaggg agacgggaca ctaggataaa gggaaaggat aaagggaacg
     1681 caacccttta tggatattta tggtgctaaa ctttatgtat atccccgggg ttgcaagaac
     1741 cgtatttgct gatttaccac accacaaaat tatcggtttc ctactgatca tagaacaagg
     1801 ttttagtata aggttaagaa acgagcccat agttttttac ttgttagaat tcgggcagta
     1861 ttttttgaag aaagaggttg gcgattctct ggtggccttc attgctggga tgaaagccat
     1921 cctggcttac caacatatcg tcaattggtt ctgcaaatag gtcaacgagt gggatatcaa
     1981 attcaacggc tagagattcg attatggcat taaaggcact tatccgttcg atagtgacat
     2041 tatcactagg gttttcaatg aatcgaggaa gttgagtaag atcggggaca tttgcaatca
     2101 ctataaaagc cgtagtctca gcgaagagtc gaactaaaac actttcaagg ttggattcga
     2161 attcttcggg attaacacca ctaataatat cgttcgcacc aacccagata gtaatcaaat
     2221 cggggtttcc gaccaatcct gagccgattt cagataaaag catatctact gcttcattaa
     2281 tttcatctag ctctgcggct agaataccta aattcctcaa ttctacattg aatgattcct
     2341 cttctaaggc atctctgatt ctaaagacat agccatttgt aaacggaatg gcccctattc
     2401 caactgcgtc gcttgcccca attgcaacat agatgatttc taagtcttta ccaccttcgg
     2461 ttgaactatc gcaaccaccg aactgtgtta aactaattac gaaaattagt aatagtccta
     2521 aaaatctctt catttattcc ctatggctcc tacagtttat ataatatcca atgattcaaa
     2581 tagtaaataa tttttaacct tttggtattg aaatttttca taaaagaatg aactttaaag
     2641 tttcattaaa agaaaataaa agaccttatt agtaaaattc tgtctactgc cgaattaaaa
     2701 atataagttt tcgagagata actttttata ggacattagc aaaacaaata agctgtaata
     2761 gacacaactt aacctgacac ccttattttt tcacaaagtt gtctgcaagg aataatctac
     2821 catcttctcc tccttgctgt ctaatcacta taaacccagc tacttgcaat gctttaataa
     2881 tttctgtgta ggaaagacct ggaattccat tcaaatcgta atctctttaa caatgcggct
     2941 ctccgtcatg aacatttcag cttcgatcgg ttctaaataa agttccgtgg cttctttaat
     3001 attagccggt gcctcttctt ccatttctcc ttcaccgatg catccgggaa gagaaggcac
     3061 atccatcggg aattgaaaga aatgggctga acggtgttca gcccctacag aaagatgtgg
     3121 aaaatcatat aattccattc ggggagttga aactgcctgt gctgaagtct cttcacttga
     3181 gtatatactt atggcgatac ttaattcttt cgctggggaa tttgatatgt ccaaactgct
     3241 ttcattaaaa ttacgggata aggtgtttct ggatgctgaa gtaatcctta gttggagtca
     3301 aaagcccaga aatgcctatt tcaatgaagc cattaacctc tataacaagc tctggaagcg
     3361 aaagcttctg aagaaaacct tagcagaaga atccgcactg gttgcagatg aatcccttct
     3421 agtcatggag gcattcgaac tactcgaaga tgaattggat gagtaaatcg ttatggagaa
     3481 caaaaagtgg catgtttacc ttgcgaatgt taacccgcgg acattcgtga gcctcagtcg
     3541 agcgatggga acggaacctg ggaaggttcg tccagtggaa gttgcccaga tcgatcttct
     3601 gaatggacta catccatcaa cagttgtatg tcccttaact acaaaagtcc aaccaagggc
     3661 gcgtttccta agggtgcatc acactatcgg cgaaaccggg ttaaaggaac agtctgatat
     3721 tattgtagac cagattcgag ccatttataa tcgccgtttc ctcggtcata caggaaaatt
     3781 tctcgtaaaa gccaagtaaa gctatatgag atattcggat tttgctgaca taagcaatag
     3841 cgtagttcag gtgttatagg ctctgttctt ctacaatcct ttgagactct agaataattt
     3901 taaatctaag cacctttctt acataagctg gaatccccct ttcaaaacct gatgcattta
     3961 aaaagcttgg tcctgcgtta taagcatgca gagcccattc aacgctttta aaacgatcaa
     4021 tcagcataga aagataatgg actccaagcc tgacattaat aaagggattg taaagggatt
     4081 ttatcccatc tatagaaact cccagctctc ctgctataaa ctctgcagtt tcaggcataa
     4141 tctgcatgag gcccattgcc cccttaggtg atactgccct gggggcgaat tcactctcaa
     4201 cttgaattat ggctaggatt agaaatggat caagcccaca attttgggac tcttctctaa
     4261 tgagtttagc aagtttcatc gcatcgtagg ttgatatggc acttgaaaat ctaagaataa
     4321 ccctaaggat ttcgatttct tctctcaaat attctggatt agctgttgtc tcttgtattt
     4381 ctgagtaaaa aaacttacgc tctttgaatc ttgatactgg actatttaac acatttaaag
     4441 aagctaggct ttctttatag tggatatgca cagattttac aaacgagata gcgaagagaa
     4501 ataataatga aagaaaaaaa gaaaccttaa aaaggcttat aagggaaaaa gtaatatgat
     4561 cacaaaattt cctgaacaat atacaccccc tagactccgg ataacggcca cttccttgaa
     4621 attaatctgt tagattttat tctcttttga acttgataat tccccgtagc ttgctaccgg
     4681 ggtttccaat gtcattgcga gcaatacgag aaatcttaga ttcctcactg ctccttcgct
     4741 tagactcagg acagggttcg gaatgacact ccctgacaga ttgcttcgtc cccttcgctg
     4801 cttttcattt ccctttgaac cacactgcgg ttcaaagaaa gcttcgctca ggattcctcg
     4861 caatgaacga aggtaatgtc tgttcatatg tcggtaccct gcgacttgct gcagggttct
     4921 tcattaaaag gtctagcgtt agagagtggt cttcataatg aagccaccct caataaatat
     4981 ttcactaagc agaccacatt agatgttcaa gcacctgttc ttctctttct tctagaaccc
     5041 taattatctg gtctgtcaat tcttcaccca gcaaccaaga ggatttttta gtttccttct
     5101 ttattcgttt aatttccatc aaaatctcat ttatgttcat tatcatgtct tcttctcttg
     5161 ctttcatttt tttcacctcc gtttcgtcga tcgttacact tggtcttaat ttccatatag
     5221 tttcaatcta ttaatattag gctaattggt aatttttaag gggttgttaa acctagatta
     5281 gtttaaattg aagggatgaa actccctcta aacatagagt tacctttcag aagtggtgta
     5341 atccaaattt gagtttagtt gttccttaac actatttttt aaaactgttt gattttcttc
     5401 aattttatgc aatgtatgtt gtaagacgtt ttggtatttt tccctcacca atcctgcaat
     5461 tccttttacc ccgtccgaga aatcctttac tttagtatta ttatcccttt tgttcatagg
     5521 tttatactta ttgtagtaat acagttttat gaaaaatata cacggttaaa agggtctgtc
     5581 aataaaatat ttcgcaaatg cgtataatag taaatattat catcatggat aaaatcagga
     5641 aaaagcgggt atatcaattt tttctctacg cacttgctaa ttatgatatt gggaaattcc
     5701 ctattttata caagaagaac gtcttagtgc ttgcaaatat aaaatttaat ctttatggtc
     5761 tcgaatatag gatgaatttt cgatcactaa cttattcaaa aaatccattt tttttacaga
     5821 taggtaaaga cctaaaacct ttaatatttg acatccaaat tcttaattcg gctctgtttt
     5881 gtacttaaat atgttctgtt cttatttttg agaattaatt gggagcttac agttcttttt
     5941 aggtttctaa gtttatattg aaaacattaa atttatgtaa taaaaattaa gtgggaatga
     6001 cccatttttt agggacattc ccactattaa aggaggatgg aggatgatgc caaattaagt
     6061 ttacaaatta attttaatta taaaaataaa gtatattact ttagttgtca aggggtaatt
     6121 tcccaggttg taaaatttat tttaatagta atcttttaca gcttgatttc aagcacagaa
     6181 ctaattaaag agagatttgc cttaatgtag gtagcgatct tcctctgatc aaatctagtt
     6241 atctagtcgt taaggtttta cttattaata ttaaaagttt cttctttcgg tgtttcacgg
     6301 aagccatgga tttgaatgtc ttctcgttgt gattgtagct cttttatttt gttgtccctc
     6361 tctttaattt ggcttcttat ggaaactgca tagaacgcgc cccaggctat ggcaaaaaac
     6421 acgccaatta aaaatgcaca cacaagcagc actatattag aagtttcata tggaccaatc
     6481 tgataaactt taagatcaag tttgaga
//
LOCUS       DATDRL010000120        10264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2309925, whole genome shotgun sequence.
ACCESSION   DATDRL010000120 DATDRL010000000
VERSION     DATDRL010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10264
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309925"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VI935_04145"
     CDS             <1..350
                     /locus_tag="VI935_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLE24825.1"
                     /translation="ILISDETYKLAKEEVIARELDIIRVVGKTEPVKIYELVSQKGEL
                     DDRKLGILEKFHIGVNLYRERKWSKAKEYFEKILELDPSDKPSQEYLRRCDEYENSPP
                     PDEWEGVFELRSK"
     gene            complement(381..620)
                     /locus_tag="VI935_04150"
     CDS             complement(381..620)
                     /locus_tag="VI935_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HLE24826.1"
                     /translation="MEFWEGERCEYCNGLIVEKKVNLSRKVKGKYVLIENVPAGVCKG
                     CGTRYYAANVLKTIEETIRGRRKAKREVLVPVYSL"
     gene            complement(638..913)
                     /locus_tag="VI935_04155"
     CDS             complement(638..913)
                     /locus_tag="VI935_04155"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HLE24827.1"
                     /translation="MSKIGEIRTLVRNGLYYLTEHADEEAMAENFDIYDIEYGILTGK
                     IRRTWPREGKYEVVSSALDGRPIGVVCRITQTGKVRVITVYEDQPKV"
     gene            1022..1216
                     /locus_tag="VI935_04160"
     CDS             1022..1216
                     /locus_tag="VI935_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE24828.1"
                     /translation="MGQSKVGVNLYREQKWVGAKEYFEKILELDPSNKPSQEYLRRCN
                     EYQTSPPPEDWEGVFKLRIK"
     gene            complement(1288..1983)
                     /locus_tag="VI935_04165"
     CDS             complement(1288..1983)
                     /locus_tag="VI935_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HLE24829.1"
                     /translation="MFKSNFYCEDEGYTVDEMKVELERHEKLESFELERSSERFDKMI
                     DTDFLNRLPMDDVKVRVSIRNQKVQTLYNDAKKEYDDWLLKGKSAVDQAAKELLKPGE
                     DIRQIEGFIGKIPADFIGGGSFESSPIYKKWRTRIEEAKKRLENAKKILGVKAHEKFG
                     FPDIHEEQEGYTLTDEGEQTKGRRETIPDDVKMYVWNRDGGKCLKCGSQEKLEFDHII
                     PLSKGGSNTARNI"
     gene            complement(1987..2169)
                     /locus_tag="VI935_04170"
     CDS             complement(1987..2169)
                     /locus_tag="VI935_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24830.1"
                     /translation="MFKKDSSCGFAKFNAGDSSYVYSFRNGFFQAPMLSESDYKKLLQ
                     HQENTPEVVMRLEGKT"
     gene            complement(2249..3283)
                     /locus_tag="VI935_04175"
     CDS             complement(2249..3283)
                     /locus_tag="VI935_04175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLE24831.1"
                     /translation="MSHIKFGIVAPAGEEPRRLLDFIKGAEDSGFDSFWAGDHMVFTS
                     KPRAFDVLSTIGAAGIITKKIFMGSISDSHKLHPAVFAQRLATLDHLTDGRFILGLGL
                     GEAMNLDPYGIKWNKPLSRMVESIKLISELWKSREPFDFDGEFFQFRNAFLDARPING
                     KVPIYIAAHRPRSLRVAAEMGDGWITLPQPPFLFAKKRKQVEELRRSSGQNLDNFEKC
                     QYLFTSVANNKESAFQALEHLKHILIWSELYEEAYGIKLPEEFRDCSYMNVLITDEIG
                     KQKLRELGKLFSKEAVLDFTLSGSAKDCIKRIEEYIEAGANHFLIHNFSADPEWSYKV
                     LTEEVIPYFR"
     gene            complement(3988..4197)
                     /locus_tag="VI935_04180"
     CDS             complement(3988..4197)
                     /locus_tag="VI935_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24832.1"
                     /translation="MARQRAKKRARYIHRVKRPSAFRNPGGEITEVDLLRLSDRTGLS
                     YHEIHNIYESMKNSKQVKPAKARRK"
     gene            4487..5110
                     /gene="dps"
                     /locus_tag="VI935_04185"
     CDS             4487..5110
                     /gene="dps"
                     /locus_tag="VI935_04185"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008453139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA protection during starvation protein"
                     /protein_id="HLE24833.1"
                     /translation="MAKEKRHMKGRVKPGDVTQRVGVEQIEARGVNVKQLREKLIDAA
                     GAEFTTYYYYTILRMHLAGHEDYKEICEDARLEDRAHFELITPRIYELGGGLPRDIRE
                     FADRASCLDAYLPENPTAASILQILLEAERCAIRTWNEICDLTFGKDPRTYDMASRIL
                     QEETEHEAWFIELLSKERDGEIRPSGHFRRGEPGEAPYSKNRSFYNP"
     gene            5134..5466
                     /locus_tag="VI935_04190"
     CDS             5134..5466
                     /locus_tag="VI935_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HLE24834.1"
                     /translation="MKTYEVKLITPDGEVRLEVGEEEYILDAAFEVGYNLPSMCLQGF
                     CLTCASRLLDGDIDQSDAIRYYPEDKKAGFVLICSAKPRSNLCIKTHQKIEMQHHRDE
                     FGLPYPRG"
     gene            complement(5771..6490)
                     /locus_tag="VI935_04195"
     CDS             complement(5771..6490)
                     /locus_tag="VI935_04195"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HLE24835.1"
                     /translation="MNIEDINRLNQEFRTKSPEEAVRWTINNLHPNVALASSFGAEDV
                     VVIDMMMRINPEARVFTLDTGRLNQETYDVMDEIRNKYNINIEVMFPNQDEVENMVRT
                     HGMNLFYKSIDNRKLCCGVRKVHPLNRMLETLNGWITGIRSDQTQNRAGADKFELDDQ
                     HGDILKVNPIIEWKWEQVLDYIEKNNIPYNKLHDKGYPSIGCEPCTRAIKPGEDLRAG
                     RWWWENDPQKECGLHVAHGSR"
     gene            complement(6564..7733)
                     /gene="sat"
                     /locus_tag="VI935_04200"
     CDS             complement(6564..7733)
                     /gene="sat"
                     /locus_tag="VI935_04200"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HLE24836.1"
                     /translation="MEGSINPHGGRLVNRTLEGKEREEALERARSLKSILVSSRILSD
                     LELISVGAFSPNEGFMGKEDYESVVENMRLKNGIVWSIPLTLSVSKEEAVDLKEGRDI
                     ALIDESQIPIAILRLEERFSYDKQKEAKEVYRTTDEVHPGVSVLYSQGDVLLGGKISL
                     INNPPYVEFPKYRLNPVQTRRIFREKGWNRIVAFQTRNPIHRAHEYLQKCALEMADGL
                     LIHPIVGETKSDDVPAYVRMKCYEALIENYYPKDRVLLSVLPAAMRYAGPREAIFHAL
                     VRKNYGCTHFIVGRDHAGVGNYYGTYDAHKIFDEFKPEEIGITPLFFDHAFYCKRCAS
                     MVTTKTCPHPSDFHVHLSGTKVRELLKSKKDLPHEFTRPEVGSILMKAYENDRQS"
     gene            complement(7919..8734)
                     /locus_tag="VI935_04205"
     CDS             complement(7919..8734)
                     /locus_tag="VI935_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HLE24837.1"
                     /translation="MVYFSCSKYRANNSAASMATAYGAGARSKREAIILIAIFALTGS
                     LVAGAPVIKTMGKGLVPESVLSSHLGFVLIILIIAIFFISWANLVRVPVATTHAIVCA
                     IAGVGLYSSGLNYKKFFEIVIWWVVTPFVAWFFNYLVGRYLYFRTLRFLTNRYSEDGI
                     RRILGFLITISGCYVAFSAGANNAANSVGPLVGIGLIGPSFGAILAGLGMGIGAMLLG
                     GRVLETVGKQITDICILRAVSVEFVGATIIIIASVFGIPVSLAEIVTSGIIGF"
     gene            8939..>10264
                     /locus_tag="VI935_04210"
     CDS             8939..>10264
                     /locus_tag="VI935_04210"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLE24838.1"
                     /translation="MTSQVYLLTKDQKLINEVENSLPENVELTKSDEAKSAKGADAVL
                     LDVDSVGISALNRLKNESFIIIITAQKGSRYLIESMTFGAFDCIFRPVDRSTLLETLG
                     RAIGIRAELESNLIKFPGEIEGSSVTCAIVGNSPTLQEVCKLIGQVGRVEVPVLITGE
                     SGTGKDLVAESIWKVSTRWEKPFVVINCAAIPETLLEAELFGYEKGAFTGATSSRIGK
                     FEEADGGIIFLDEIGDMSLSLQSKVLRVLQNGTFYRVGDNKEVKVDIRVIAATNKDLE
                     EMVSQGKFREDLYFRINVVRIHLPSLRERKEDTPLLVECFTRRYSSQTGKDIKGVSKR
                     FLDKLMEYDWPGNIRELENTIRKAIVFTKTPYLTSYDLNLNKAISFQETKETSFTEPL
                     RNSVRNLLRSRGKNGDVYSQILKEAERILLEEALNASSWNRSKAARLLGI"
BASE COUNT         3069 a         2280 c         1925 g         2990 t
ORIGIN      
        1 aaattcttat ttctgatgaa acctacaagc ttgcaaaaga agaggtcata gcaagggaat
       61 tagatattat tcgtgttgtg ggtaaaacag aacctgtaaa aatctacgaa ctcgtttcgc
      121 aaaaagggga attagatgat agaaaattgg ggatcttaga gaaattccat ataggtgtca
      181 acctttaccg agaacgtaag tggagcaagg ctaaagaata ttttgaaaaa atactcgaac
      241 tagacccatc cgataaaccc tctcaagagt acctgagacg ctgcgacgaa tacgagaact
      301 ctccaccgcc cgatgaatgg gaaggtgtgt ttgagcttag aagtaagtaa accaatcctt
      361 tctcgaatga ccacttaaag tcaaagcgaa tagacaggca caagaacttc tcgctttgcc
      421 tttcggcgtc cacgaatagt ctcttcgatc gtcttgagta cgttcgctgc atagtaacgt
      481 gtaccgcacc ctttacagac accagcaggc acgttctcga taagcacata ctttccttta
      541 accttccgag agaggttgac tttcttctcg acaatgagtc cgttacaata ttcacagcgt
      601 tcgccttccc aaaattccat tgcagtttct cctttcccta taccttgggt tggtcctcgt
      661 aaactgttat aacacggacc tttccagttt gggttatacg gcatacaaca ccaatgggtc
      721 ttccatccag cgctgagcta accacctcgt atttgccttc tcgtggccaa gttcgacgaa
      781 tcttgcccgt taggatgcca tattctatat cgtagatgtc aaagttttca gccattgctt
      841 cctcgtcggc gtgttcagtg agataataga gcccatttcg aacgagtgtc cgaatttccc
      901 cgattttgct caatgagagt ctccatcaag gtcatcaagt tcaaagataa gccatgttat
      961 actaaccaaa gaactatttc aaaaagaagc cttgtgcaac aattttactg tggaattgac
     1021 catgggtcaa agtaaagtag gggttaatct ctacagagaa cagaagtggg tcggagctaa
     1081 agaatatttt gaaaaaatac tcgagctaga cccatccaat aaaccctctc aagaatatct
     1141 caggcgttgt aacgaatacc agacctcgcc acctcccgaa gattgggaag gagtatttaa
     1201 gctaagaatt aagtaattgt ttgtatttct tgaagtttct cagacaatag aatctccttt
     1261 agagcgattg caggtttcgc aaagaagtta aatattgcgt gctgtgttac tgcctccctt
     1321 agacaagggt atgatatggt caaactcaag cttctcctga cttccacact tgaggcattt
     1381 cccaccgtcc ctattccata cgtacatctt aacatcgtca ggtattgtct ctcttctccc
     1441 cttagtttgc tccccctcgt cagttaatgt gtatccctct tgctcttcgt ggatgtcagg
     1501 aaagccaaat ttctcatgcg cttttactcc taagattttt ttggcgtttt caagcctctt
     1561 tttcgcttct tcaatacgag ttctccactt cttataaata ggactagact caaaactacc
     1621 accacctata aagtcagccg gtatctttcc aataaacccc tctatctgtc taatatcttc
     1681 accaggtttg agcaattcct tagctgcttg gtctacagca gattttcctt tgagcaacca
     1741 atcatcatat tccttttttg catcattgta taaggtttga actttctgat ttctgataga
     1801 taccctcact ttaacatcat ccattggcaa gcgatttaga aaatcggtat caatcatttt
     1861 gtcgaatctt tcactcgacc tttctaattc gaatgattct aatttctcgt gcctttctaa
     1921 ttccactttc atctcatcga ccgtataacc ttcatcttca caatagaaat tactcttaaa
     1981 catccatcaa gttttacctt ccaatctcat aaccacttca ggcgtatttt cttgatgttg
     2041 caacaatttc ttataatcac tttcgcttag cataggtgct tgaaaaaatc cattccgaaa
     2101 gctatatacg taactagaat caccagcatt aaatttagca aatccgcaac tactatcttt
     2161 tttaaacata taactttact ctatcaacac ttttgtttct tgcttaacta ctacctaaag
     2221 catatatcct aattaccaaa acccaatctc acctaaaata tggaatcact tcttctgtca
     2281 agactttata actccactca gggtcggcgc tgaagttgtg aattaggaaa tgattagcac
     2341 cagcctcgat atattcttct attctcttta tgcaatcttt agccgagcct gaaagggtaa
     2401 aatcaagcac cgcttctttt gaaaatagct ttcctaactc tctaagcttt tgctttccta
     2461 tttcatccgt aataagcacg ttcatgtaac tacaatctct aaattcttca ggcagtttaa
     2521 taccgtaggc ctcttcatag agttccgacc agattaaaat gtgcttaagg tgttccaaag
     2581 cttgaaaagc cgattctttg ttatttgcaa ccgatgtaaa tagatattgg cacttctcaa
     2641 aattatctaa attctgccct gaactccttc tcaactcctc tacctgcttt cgcttttttg
     2701 caaataaaaa tgggggttga ggaagggtaa tccatccatc tcccatctca gcggcgacac
     2761 gaaggcttct cggacggtgt gcggcaatat agattggaac ttttccatta attggccttg
     2821 cgtctaaaaa agcatttctg aactgaaaaa actcgccatc aaaatcgaaa ggctcacggc
     2881 ttttccagag ctcgcttatg agttttattg actcaaccat gcgtgaaagt ggcttgttcc
     2941 actttatgcc ataagggtca aggttcatcg cttcgccgag cccgaggccg agtataaatc
     3001 ttccatctgt gaggtggtca agcgtagcaa gacgctgagc aaacactgca ggatgaagtt
     3061 tatgtgaatc agaaattgaa cccatgaata ttttctttgt aataattcct gctgcgccaa
     3121 tcgttgataa aacgtcaaat gctctgggct tcgatgtaaa gaccatgtgg tctccagccc
     3181 agaagctatc aaacccggaa tcctcagctc cctttataaa atccaaaagc cttcttggtt
     3241 cttctcctgc gggcgctaca ataccaaatt ttatatgcga catgattgag tgtagatttt
     3301 acactccttt tggtataggg gaaattaaag gcgtgaaaat ttgaaagaaa gattaatgaa
     3361 gaaccatgca gcaagttgca ggatatcggc atggaaaaga tagtctcttt ggtcattgta
     3421 atgaaagtac ggaagtgtct ttgcgaggag tgaaacgacg aagcaatccc atcgagattc
     3481 ctcactgtgt tcggaatgac actttgtgtc agattgcttc acggaatatt tcgataggcg
     3541 gggttttcaa accccgcatc ctgttgcggg acaagcctgt cctgagttta acgaaggaaa
     3601 tttcccgcct ctccctctcc gctgcaggct ccgtgaatgt gttcgcaacg acagttgaaa
     3661 ccctttatca agcacaagga attatcaagt taaatgggaa aggtaaacct aataagtttg
     3721 acagttaaaa ctgctctata catctaagat tgaactaaaa cattaaatgc agcaattata
     3781 caatttctta aggaaaagtt tttaaaatgc caacggaatg tcattcccgc attctaccca
     3841 cttaatgcat gtgggtacaa gctttagcgg gaatccagta tataagtcga ttctagatac
     3901 ccgataaaga cactcgggta tgacaaacca tcaaatgccc ctaaagtgag taaacaaaat
     3961 ttgaacactt aatagatgga aatggcatta cttccttcta gctttagccg gctttacttg
     4021 cttgctattc ttcatagact catagatatt atgaatttcg tgatagctca ggccggtcct
     4081 atctgaaagc cttagcaaat ctacctctgt tatttctccc cctgggtttc ggaaagcaga
     4141 tggtcttttt actctatgaa tatagcgagc tcttttcttt gctctttgac gagccatttc
     4201 attcaccccc ttgcatcatc tctccaagaa tggttcgttt atatctgaac ggtaatgatt
     4261 accatatagc ataataatac cgttgtcaag ggctattgtt aagcacttta tacatcccaa
     4321 gtgtaaagac aaaaatatca aacgtgaata atgatgaatt tagtcacact gttgtaatag
     4381 gcaagatgtt tattttcttc tacttaataa tcgttactat ttacaaatta tctctaacaa
     4441 atataataac ttctagaatt acaactatat aaggaggcat aagaaaatgg ctaaagaaaa
     4501 acgacatatg aagggaaggg taaaaccagg cgatgtcaca caacgggtag gagtggagca
     4561 aatagaagcc cgtggtgtaa atgtcaaaca attgcgcgag aaattaattg acgcggccgg
     4621 cgctgagttt accacttact actattatac aattcttcgg atgcaccttg caggacacga
     4681 ggactacaaa gaaatttgcg aggatgcccg ccttgaggat cgagcccatt ttgagcttat
     4741 aaccccaaga atctatgaac ttggtggggg gcttccaaga gatattagag aatttgccga
     4801 tcgagcttca tgtcttgatg cataccttcc tgaaaaccca accgccgcaa gcatcctaca
     4861 gattctcctc gaagccgaga gatgcgcaat caggacctgg aatgaaatct gcgaccttac
     4921 atttggaaag gaccctagaa catacgatat ggcttcacga atacttcagg aagagaccga
     4981 gcatgaggct tggttcattg aacttctaag caaggagcga gatggagaga ttcgtccatc
     5041 cgggcacttc aggcgtggcg aaccgggcga ggctccatat agcaagaacc gttctttcta
     5101 taacccttaa tgaaaatctg agggtttagt cgagtgaaga cctatgaagt aaaactaatt
     5161 acgcctgacg gtgaagtccg ccttgaagtt ggagaagagg aatatatctt agacgctgcc
     5221 tttgaggtgg gctacaacct tccctctatg tgtttacaag gattttgtct tacatgcgcc
     5281 tcaaggcttc ttgatggaga tatagatcag tccgatgcta tccgatatta tcccgaggac
     5341 aaaaaggcag gatttgtttt aatctgtagc gctaaacccc gctccaatct atgtataaag
     5401 acacaccaaa aaatagagat gcagcaccac cgtgatgaat ttggtttgcc gtacccccgt
     5461 ggataggtct ttgcttctca gtcgctactt ttttatacat ttttcgaatt attaagtgat
     5521 caaatatagt acccgctcct cttataatca cttgtaaagg tccctcgaca agcctgtact
     5581 gagtttatcg aagggatgag cggaaagctt cccgctggaa ttcgtgtttt gtaatgcatt
     5641 cagagtgtag cggagacttt aggtctcctc ataaagattg gaaggcttta gccttccgct
     5701 aaagaagaaa aggagataaa attaaacccc taggattcca ctaaaattat atttttacta
     5761 tttcgctgtc tcatctgcta ccatgggcaa catgcaatcc gcattccttt tgcggatcgt
     5821 tttcccacca ccaccgacca gctctgagat cctctcctgg ttttatagcg cgtgtacatg
     5881 gttcacaccc aatgctcggg taacctttat catgtaattt attatatgga atattgtttt
     5941 tctcgatata gtccaaaacc tgttcccact tccattcaat tataggattt accttcaaaa
     6001 tatcaccatg ctggtcatca agctcaaatt tgtctgctcc tgccctattc tgggtttgat
     6061 cacttcttat accagttatc cacccattta atgtttccaa catcctattt aatgggtgga
     6121 cctttcttac cccacaacaa agctttctgt tatcaatgct tttataaaat aggttcatac
     6181 catgggttct aaccatattt tcaacttcgt cttggtttgg gaacataacc tcgatattga
     6241 tattgtattt gtttcttatt tcatccataa catcatatgt ctcttggttg agtcttcctg
     6301 tatccaacgt aaaaacccta gcttcaggat ttatcctcat catcatgtct ataacaacta
     6361 catcctccgc cccaaaactt gaggctaatg caacatttgg atgaaggttg ttaattgtcc
     6421 aacggacagc ctcttcaggg cttttagttc tgaattcttg gttaagcctg tttatatctt
     6481 cgatattcat tgtagtctcc ttttactttg tgattcgtgg ttcgattcac aatacacacg
     6541 atccacgaaa cccgagccac gattcacgat tgacgatcat tctcatacgc cttcattaaa
     6601 atactcccta cctccggcct tgtaaactca tgcggcagat ctttcttact tttaagtaac
     6661 tctcttacct tagttccgct taaatgaaca tgaaaatcag atgggtgcgg acatgtttta
     6721 gtggtaacca ttgaagcgca tcgtttgcag taaaaagcat ggtcaaaaaa gagcggtgta
     6781 atccctatct cctcaggctt aaactcatcg aaaatcttat gcgcatcata ggtgccgtaa
     6841 taatttccta cacctgcgtg gtcacgtcca acaatgaaat gggtgcaacc ataatttttt
     6901 ctaacaagtg cgtgaaaaat tgcctcccta gggcctgcat acctcattgc agccggaaga
     6961 acggaaagta aaaccctatc cttaggatag tagttttcaa tcagtgcctc gtagcatttc
     7021 attctcacat aggcaggaac atcgtcgctt tttgtctcac ccactatggg atgaattaaa
     7081 aggccatccg ccatttcaag tgcgcatttt tgaagatact catgggctct atggattgga
     7141 tttcgcgttt gaaatgcaac tattctattc catcccttct ctctaaatat tcttctagtt
     7201 tgcactgggt tcaatctgta tttaggaaac tcaacatatg gaggattatt aatcaggctt
     7261 atctttccgc caagaagcac atctccctga gaatatagga cgctgactcc gggatgcacc
     7321 tcatccgttg ttctatatac ctcttttgct tccttttgct tatcatagga gaacctttct
     7381 tcaagacgta agatggcaat tgggatttga gactcgtcaa taagcgctat gtcccgacct
     7441 tcctttagat ccactgcttc ttcttttgat acagaaagcg tgagaggaat gctccagaca
     7501 attccatttt tgagcctcat attctcgact acgctttcat aatcctcttt tcccataaag
     7561 ccttcgtttg ggctaaaagc accaactgaa atgagttcta agtctgataa aattcgtgaa
     7621 gaaactaaaa tgctttttag actcctagct ctttctaatg cttcttctct ttctttccct
     7681 tcgagggttc tatttacaag cctacccccg tgagggttaa ttgaaccttc catcttttga
     7741 atttcacctc aagacattag aagtttttac ctagcatcgc tgcgacgccg tacttgaaat
     7801 aaagagagaa cataaaatag ctagcagcta cggcgaaaaa gggaactaaa aaccaaaaaa
     7861 atgcaattct taatacatga ctatttctcg ctgttgcggt aaatccttga tttgcacatt
     7921 agaatcctat gataccagaa gtgacgattt ccgcaagtga aacaggtatt ccaaaaaccg
     7981 aggcaattat aataatagtt gcaccgacaa attcaaccga gactgctctt aaaatacaga
     8041 tatctgttat ctgctttccc accgtctcta agacccttcc gccaagcagc attgcgccta
     8101 ttcccattcc caaaccggcc aagatagcac caaaagatgg acctatgaga cctattccga
     8161 ctagtggacc aacggaatta gcagcattat tagcgccagc agaaaaagcc acgtagcaac
     8221 ctgatatagt gatgagaaaa cctaggattc ttcttatacc atcttctgag taacggtttg
     8281 taagaaacct aagggttcta aaatagagat accttcctac aagataatta aaaaaccatg
     8341 cgacaaaagg agtgactacc caccaaatta caatctcaaa aaatttcttg taatttaaac
     8401 cactagagta taaacctaca cccgcaatcg cacaaacaat agcatgtgtt gttgcaacag
     8461 gtactcgaac taagtttgcc caagagataa aaaatattgc gattatgaga attatcaaaa
     8521 caaaacccaa gtgtgaggaa agcacagatt caggaacaag acccttgccc atagttttga
     8581 tcacaggcgc tccagccaca agtgatccag ttaatgcaaa tatcgcaatg agtattatag
     8641 cctctctttt agacctggcg cccgctccat aagcagtagc cattgacgcc gctgagttat
     8701 tagcccgata tttagagcaa gaaaagtaga caatactatt gctattggaa gaacaatatc
     8761 cacctcaaag cctccctagc ttaaatgaga atagactata cacattagaa tttttgttat
     8821 tacgcaaaga gaatgccaaa gagaaaatcc attgttatca atagattaaa gcaacctgtt
     8881 atctgtctta ttgattatct cttaatcaac ctgtatattt ctgtaacaca taaataaaat
     8941 gacaagccaa gtatatctac tgaccaaaga ccaaaaactc ataaatgagg tagaaaatag
     9001 tctacctgaa aatgttgagc ttactaaaag cgatgaagct aaatctgcaa aaggagcgga
     9061 tgccgttcta cttgatgtag atagcgttgg tatcagtgca ttaaaccgat tgaaaaacga
     9121 gtcttttata ataataatta cagcccagaa ggggtcacgt tatcttattg aatcaatgac
     9181 ctttggcgcg ttcgactgca tctttagacc tgttgatcgt tctacacttc ttgaaaccct
     9241 aggacgagcc ataggtataa gagctgaact tgaaagcaac ctgattaaat tccccggaga
     9301 aatagaaggg agtagcgtga catgtgcaat cgttggtaac tcgccaacgc ttcaggaggt
     9361 atgcaagctt atagggcaag tcggtagggt tgaggttcct gtcttaatca ccggagaaag
     9421 tggaacggga aaggacctag ttgctgaatc aatctggaag gttagcacca gatgggaaaa
     9481 gccatttgtt gtaataaact gtgcggcaat tcctgaaaca ctccttgaag cagaactctt
     9541 cggatatgaa aaaggggctt tcactggcgc cacttcgtct cgtattggga aatttgagga
     9601 agctgacggc ggaatcatat ttcttgatga aattggcgat atgtcgcttt ctcttcagtc
     9661 aaaggttctc agagtacttc agaatgggac tttctacagg gttggagata ataaagaagt
     9721 aaaagttgat attcgcgtaa ttgccgcaac aaataaagac ctcgaagaga tggttagtca
     9781 aggcaaattc agagaagacc tttacttccg aataaacgtt gtgagaatcc acctaccgtc
     9841 acttagagaa agaaaagaag acacacccct tttggtagaa tgtttcactc gacgctatag
     9901 ctctcaaacc ggaaaagata taaagggggt aagcaaaaga tttttagata aactgatgga
     9961 atatgactgg ccagggaata taagagaact agaaaacaca attagaaagg ctattgtttt
    10021 cacaaagaca ccttacctaa cttcttacga cttaaaccta aataaagcaa tctcctttca
    10081 agaaacaaaa gaaacctcat ttactgaacc ccttcgaaac tctgtacgaa accttcttcg
    10141 ctcaagagga aagaatggtg acgtctacag tcaaatcctt aaggaagccg agcggattct
    10201 cttggaggag gctctgaatg caagctcatg gaatcgttct aaagcagcca ggcttctcgg
    10261 cata
//
LOCUS       DATDRL010000121         2585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_491144, whole genome shotgun sequence.
ACCESSION   DATDRL010000121 DATDRL010000000
VERSION     DATDRL010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491144"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VI935_04215"
     CDS             <1..571
                     /locus_tag="VI935_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HLE24839.1"
                     /translation="VEALKEAITKLSTDKCRVKIIHGGVGAVNETDVVLASASNAVIV
                     GFNVRPDVKAIEAAERERVSLELHIIIYDVVDRIKKAMEGLLEPVIKERIVGHAEVKA
                     TFHVSKVGTIAGCYVSDGKVTRGSNIRVIRDGVLIFEGKISSLKRFKEDVREVQSGYE
                     CGIGIENFNDIKVGDTFEVYTFEEIKQKL"
     gene            689..979
                     /locus_tag="VI935_04220"
     CDS             689..979
                     /locus_tag="VI935_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HLE24840.1"
                     /translation="MVIGALRIELYIDGNRSLKGKRQMLRSVIQRVKSRFGNVSISEV
                     DSQNLWQRATIGVSFVSNDAPYVNSVLDQISGFIESMGIVQIVSRDLEIIHF"
     gene            993..1346
                     /gene="rbfA"
                     /locus_tag="VI935_04225"
     CDS             993..1346
                     /gene="rbfA"
                     /locus_tag="VI935_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HLE24841.1"
                     /translation="MGIGYKRTVRVGDLILREISQMIIKGEIKDPRVSFVVLTGIKVT
                     GDLSLARLYFTVMSDGIGKEEALIGLQSATGFIKRELWKRLRIKRIPDLRFEFDTVLE
                     VGYRIDALIKGIKVE"
     gene            1339..2349
                     /locus_tag="VI935_04230"
     CDS             1339..2349
                     /locus_tag="VI935_04230"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HLE24842.1"
                     /translation="MSDLEKIISVISTGKRFLITSHENPDGDAMGSMLALGLGLEKIG
                     KEIIYYNKDGVPLLLRFLPHSEKVISSLNDLNGGFHATFVVDCTDISRVGVKFEDLKN
                     RGGCGVTIIIDHHQTSKPSADLYILNPRASATGMIIYSLLKTLSLRLGSGQALRITPD
                     IAMNLYTSILVDTGSFRYSNTIPEAFEIAAELVQLGASPWDISQALYESEPIGKLKLL
                     GCVLPTLEVTEDGRIASVVVKLDMFKATGTSKEDTEGFVNFPRCINGVDVAVLFREEE
                     PSENGSRWKISLRSKGSVNVAQIGEKFGGGGHEKAAGCTISATLAEAKRLVFSAIKEV
                     LQ"
     gene            2346..>2585
                     /locus_tag="VI935_04235"
     CDS             2346..>2585
                     /locus_tag="VI935_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HLE24843.1"
                     /translation="MNGMIILDKEKGVTSQKVVMEVKRALKIKRAGHTGTLDPFATGV
                     LPICLGDATKLVPFLDDSIKEYEAVLRLGIATDTMD"
BASE COUNT          810 a          404 c          642 g          729 t
ORIGIN      
        1 tgttgaggca cttaaggaag caataacaaa gctaagcact gataaatgtc gcgtgaagat
       61 aatacacgga ggagttgggg ctgtgaatga aactgacgta gttcttgcaa gtgcctctaa
      121 tgccgtaatc gttgggttta atgtacgccc tgatgttaaa gcgattgaag cagcagagag
      181 ggaaagggtt tcactcgagc ttcatataat aatctatgat gttgttgaca gaataaagaa
      241 ggcgatggaa ggtcttcttg aacctgtgat taaagaaaga attgtcgggc atgctgaggt
      301 taaagccacc tttcatgttt caaaggtagg aaccatcgca gggtgttatg tatcagacgg
      361 taaggttact agaggcagca atattagggt aattagggat ggtgtcctca tttttgaagg
      421 aaagatatca tccctgaagc gttttaaaga ggatgttaga gaggttcaaa gtggatacga
      481 atgtggaatc ggaattgaaa attttaatga tataaaggtt ggagatacat ttgaagtcta
      541 tacattcgag gaaattaagc aaaaacttta gcccattgga aggtttaaat ctataaatat
      601 tttgatcgct gtgtaaagga ttaaaatagt gaattaatat ttcttagtgt tagattcgat
      661 atttatttct atatattaat attagtaaat ggtaattggt gcactaagaa tcgagctcta
      721 cattgatgga aacagatccc taaagggaaa gagacagatg ttaagaagcg tgattcaaag
      781 ggtaaaatca aggtttggca atgtttcaat atctgaggtt gattctcaga acctatggca
      841 aagagcaaca atcggggtct ctttcgtaag caatgatgcg ccttatgtaa actccgtact
      901 cgatcagatt tcgggattta tagagtcaat gggaattgtt cagatcgtaa gtcgtgacct
      961 tgagatcatc cacttttagg agaagccaag ctttggggat tggatataaa cgaacggtta
     1021 gggttggaga tttaatcctc cgagaaatct ctcaaatgat tataaaaggg gagataaaag
     1081 acccacgcgt ttcctttgtt gttcttacag gtataaaggt tacaggtgac ttaagccttg
     1141 ctaggttata tttcaccgtt atgtcggatg gtattggaaa ggaagaagcc ttaattggtc
     1201 ttcaaagtgc gacgggtttt ataaaaaggg agctttggaa gaggttaaga ataaagagaa
     1261 ttccagactt aaggttcgag tttgacacag tacttgaagt gggttacagg atcgatgccc
     1321 ttataaaagg aataaaggtt gagtgatcta gagaaaataa ttagtgtaat ttcaactggg
     1381 aaaagattcc taattacatc ccatgaaaac ccggatggcg atgctatggg ctcaatgctt
     1441 gcccttggtc taggccttga gaagatcggg aaagagatta tttattataa taaagatggg
     1501 gttcctttac tgttaagatt cctcccccac tcagagaagg tgatttcctc attaaatgat
     1561 cttaatggag gctttcatgc aactttcgtt gttgactgta ctgatataag cagggttggt
     1621 gtgaaatttg aggacttgaa aaatagagga ggatgtggag tcactattat aatagaccat
     1681 catcaaacat ctaagccctc tgcggatttg tacatcctta atccaagggc ctcagctacg
     1741 ggaatgataa tctattcgct tttaaaaact ctatcccttc gactgggctc aggtcaggcc
     1801 ctaaggatta ctcctgatat agcaatgaat ctatatacct caatccttgt tgatactgga
     1861 tcattcagat attcgaacac tataccagag gcatttgaga ttgcagcaga acttgtccaa
     1921 cttggagcaa gtccttggga tatatcacaa gccctttatg aaagtgaacc cattggtaag
     1981 cttaaactac ttggatgtgt tctaccaacg cttgaagtta ctgaagatgg gcgtattgca
     2041 tccgttgtag taaagttgga tatgtttaaa gcgactggga cgagtaaaga agatacagaa
     2101 ggatttgtga atttccctag atgtataaac ggtgtagatg ttgctgtcct ttttagggaa
     2161 gaagaaccta gtgagaatgg gtcaagatgg aaaatcagtc taaggtcaaa gggaagcgtc
     2221 aacgttgcac aaattggcga gaagttcggc ggagggggac acgaaaaggc agcagggtgc
     2281 actatttctg caaccttggc agaggcaaaa cgattggtat ttagcgctat caaagaagtg
     2341 ctccaatgaa tggaatgata atcttagaca aagaaaaggg tgttacatct cagaaggtgg
     2401 tgatggaggt taaaagggca ctcaagataa agcgcgcagg gcatacaggg acactcgatc
     2461 catttgctac gggggttctt cctatatgtt tgggtgatgc aacaaagctt gttccatttt
     2521 tggatgatag catcaaggaa tatgaagcgg tattgcggct tggtatcgcg accgacacca
     2581 tggat
//
LOCUS       DATDRL010000122        34167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_959633, whole genome shotgun sequence.
ACCESSION   DATDRL010000122 DATDRL010000000
VERSION     DATDRL010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 34167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34167
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959633"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VI935_04240"
     CDS             <1..871
                     /locus_tag="VI935_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018400118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24844.1"
                     /translation="VGRIQNFKNWSPYMVPDQDKTCLGLEYFCFEGDGLWTMSNQELI
                     ELGKKELEILGLVQPSEVEDGTVVRMPKAYPVYDSTYRESLQVIRQFLDQFQNLQLIG
                     RNGMHKYNNQDHSMLTAMLAVKNILGANYDLWQVNADQEYHEEIREGEGKELVDYEHL
                     ASTQPQVPERRLPPSYDEVIIKAFARMDKFALATALGSVCGLAIFIATLLLIMKGGKV
                     TDLNLQLLRHYFIGYTVTVKGAFIGMGYSFFWGFVWGWLAAYLRNLFLGYFIHRAKSK
                     AESSSFRNLLDYI"
     gene            900..1232
                     /locus_tag="VI935_04245"
     CDS             900..1232
                     /locus_tag="VI935_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24845.1"
                     /translation="MTNNNLLEEEKLLSAVVRLNAKMLGLILGLLFGLLIFLATNWLI
                     IKGGPITRSGKEIVGPHLQLLGQFFIGYSVSFLGSIIGFLYGFAIGSICGTFIGWIYN
                     WVVDLKKG"
     gene            1583..3796
                     /locus_tag="VI935_04250"
     CDS             1583..3796
                     /locus_tag="VI935_04250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE24846.1"
                     /translation="MRRILYAYPEVSPIFPNGGIGTFVFEVSHLLAASGEWDVDILTD
                     TSYSATIMQDDFRKAETVFKQAGVRLIDLYRDERIKPGWESPDVIRAERYHAHVTKLH
                     SERHYHLIEFPDWRAPGFFVVRHKKTTKSFGNTLLVVRLHSSTKDVWDWQDGYFSNKN
                     DLYCHFMEEYVKKYSDIVMSPTEFLLRPVRQTQLVFEKLLFRDGYPISSNYQPHQKDI
                     HHDRRSDPITVACVSRLELRKGQDILANAIRHLSESNSLDNNVQFVFCGKDNMGLVRE
                     RWMSQSIKQILKGVKNWKIIKPKSRQDLMNWLATEVDICVVPSRVDNYPNVVMEASGA
                     GCYVIASDSGGIPEIFEDYEIAGALFPPGDILALEREIRKAIQYLKSNPGIRVHIAEE
                     FEKSRQKKASQTIAKYDEIIELGKKHLASSTIESATPCVSIIIPFYNAHKYARDAIES
                     AFTSEYPDFEVILINDGSDNPKSLEFLKEIESEFPKLKIIHKSKGGLGDARNAGLEAA
                     TGEFIVPLDTDNMLLPNMLSSCSRVLTEHQNLAYITTYFECLREEDGARLPLQLSPIP
                     RPLGTVDGLILLEPTPGYAPAMLRLSALKSVGGYASGIYCMEDWDLWLTFHEKGMEGD
                     VLPEVHYIHRLRSDGISNSLNALNDIRLKQSLLRKHECLVKEYSLAITTLLLNEYRNG
                     WRGQDEDWIVLKDMFRRAMNDPVRALKYVTMKIKHSLKERVRNFSPIKALMKSQE"
     gene            3891..6329
                     /locus_tag="VI935_04255"
     CDS             3891..6329
                     /locus_tag="VI935_04255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HLE24847.1"
                     /translation="MNDKAKNHIVIVEAYEGFIEFISDEPEERMAALGLGLPLEEIAE
                     DLDYRITILSWDEWYQYEVKKTWVSTPATRPASKPDFGIPLKVFKSNKIKRITVPARD
                     PEHRLPFDIKDLDPRSYGLIYDSGLLHFVSYCLSKELQDFYRNNPFQAVILPMWGGLG
                     YVSQMAKSTEVPNCISVPFAVVVTDRSATRQLANQEGIWTRQAIVRRQMEDVSLALAD
                     LVLVFGPRGADIANAGRLPEASPPVYAPRFVERLLLDKIANESMQYRDVGKPVQFFLY
                     EPQEAASGVLTTLDAVTLLRNKGVRLDLPLITAGPPMVFAPMKPRGFVDYWSSRGAVQ
                     ELVRERQWEWRREYPDFNQVFPVRLYPSYFEHLPNVWTELARGSLVLLSSAAAEGLAP
                     GQILPQEVIIEGDPVPDKLADCIERISRTEVKKLDMVRRELCMRVAAANSRVRQRQLL
                     KETTNALERLIQFAPEPQDLSRVALMFFDRRIPLKQIAQNDKTPPLPELRPGINKGTL
                     SVAVTCYEMKSMIREAVESVWASERQPDEVLLIDDGSHGEETLANIRKLEKKASERGL
                     PLRVIRQRNKGLAGARNAGLEAAKGEFISFLDGDDMIEPQFYRLAMRIFTRYPRLGGV
                     AAWAFIFGEATPDGFWNAPQAELPFLFIEDSVFVPCVTRAELLRNIGGYDTRLRYNYE
                     DWELAIRMLVSGCPIVTIPMHLLKYRIRRGSLFRSMTHVQNQVMRELLLTNHRDTVSK
                     FAVEIAMQLENLWMKQRYPDNQRDALLNYNSRPNDEIFIPQKLYSLYRLLLPKPFKII
                     LRKTQRILNSISAD"
     gene            6426..7388
                     /locus_tag="VI935_04260"
     CDS             6426..7388
                     /locus_tag="VI935_04260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015956202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE24848.1"
                     /translation="MSIIISAIICTHNRAEYLTKAIQSLVGQSISKDRYEIIVVDNCS
                     TDFTKEVVERFSSKSDIRYIYEPTLGANYARNTGWRNARGKYVAYLDDDTVACHIWLD
                     KILEVFETVTPRPGCIGGKVEPIWEIARPKWLSDWLLHGLALIDWSETPRVLSNISVE
                     WLVSANIAFPVEVLERFGGFHSGLGRLGKNLLSGDEILLEKQILKSGYACFFHPEIAV
                     RHHIPKSRLNQDWFIRRYFWQGVTDATMQLLEEAPSTTKRILIAMSKTLGLILTPRKL
                     VNLIMPVNDPGRFTDKCFALITLGHILGLLGSPTKSRRLKNNSP"
     gene            7535..8611
                     /locus_tag="VI935_04265"
     CDS             7535..8611
                     /locus_tag="VI935_04265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLE24849.1"
                     /translation="MAINIHLIRTHYPHWGQYSGINQFLKYINQNSYHVDLHLSNDND
                     EDFPIRNRFVQRCLRYFVRRGMQQYKLSDLTAEIKAFRKCFQNKVDIVHYLDGEHSAH
                     YLPWLFKKWNKFTTKILATFHQPPDLLDSLIIKDVVAKLDFVTVLSPDQVAYFGELLA
                     PDKICLIPHGVDTNFFKPRIKPKEESKFKCITVGHWLRNFKAMREVANKLVSHKNIEF
                     HVVASRLHGPRLIGLEDLKNVTIYRDNIDDTRLLELYQESDVLFLPLLQSAANNALLE
                     GVACGLPVISTLLPSVKFYLPGKEAILIKDSDTKKFVDAILYLVDNSQDRIDMGREAR
                     KRAEDLDWRNITPQYENLYSKLIT"
     gene            8963..9778
                     /locus_tag="VI935_04270"
     CDS             8963..9778
                     /locus_tag="VI935_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24850.1"
                     /translation="MIEKNLTILSYGPKKINCSYDQSCNISCPSCRTEIIIESKNKQQ
                     IMKIQNKIQNEALKDADFLYITGSGDPFGSPFFRKWLQTMKKEDMPNLKDIHLHTNGL
                     LWTPKMWGTISKDIQELITSTEISIDAATSETYSINRRGGIYEKLLENLEFISTLRKN
                     GPLKFVTISMVVQENNFREMPDFVRLGKHFNFDTVYFSKISNWGTFSEKEFRNRAVCS
                     PNHPKYSELVDLLKNEIFKEPVVYLGNLSEIANSAKGSFVPALKNRIFTFLAN"
     gene            9828..11078
                     /locus_tag="VI935_04275"
     CDS             9828..11078
                     /locus_tag="VI935_04275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE24851.1"
                     /translation="MDVSVIIPAYNAEETISDTLESLISQTYPNWEAIVVDDGSTDAT
                     SEITLGFIGRDARIRMIQQPNGGESAARNSGIALARYEWLLFLDADDLILPSHLEHLT
                     NELLSNPELDAVICGSARVALDGTLVIENYMPPVNDMFSTLARRAAFPINACIVRRSL
                     IETVGKFDTSLKTGADWDFWQRIARKGVCFGVVNEVLSLYRMRPNSATSNAYQLFKDG
                     LRLLKQGHSPDPRVTAPHPDYVNGLSPEGVNSQVFYLLTWCAGLLLGRGEDPRPLLET
                     IKEHKYPELYPDAIAKIIFESTILPKCRPTHTWEELWSSIKQHLDEFLVALEAQSLTP
                     DLARRAHIELKKLILKHSPTWQYAIEEYEQAIAILEEQRRSWQGLAEERESKIMKLQE
                     KFWVRLGLRLERALKQQILKSEKS"
     gene            11415..11855
                     /locus_tag="VI935_04280"
     CDS             11415..11855
                     /locus_tag="VI935_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24852.1"
                     /translation="MAFLDGHGTIEDWGCGTAYAKNFVKKSKYIGIDGSQSTFTNKVV
                     DLREYASKTDCIFMRHVLEHNYGWRKILANAVYSFTKRMVLIIFTPFTDVTQQIATNY
                     CPNVPDISFKKDDLTEFFKQFKYTEESLETETQYKTEHIFYIEK"
     gene            12053..12184
                     /locus_tag="VI935_04285"
                     /pseudo
     CDS             12053..12184
                     /locus_tag="VI935_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002745248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
     gene            12844..15030
                     /locus_tag="VI935_04290"
     CDS             12844..15030
                     /locus_tag="VI935_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24853.1"
                     /translation="MNNPEKKSSKILFINIALLLIVILIILITASQYISREKTIYYWD
                     YANYENRTKTILDYPQGSVKEFFFAVPKETWLSTGEDYSNIPSIPLLPVMLTFGDSRT
                     VFILGMALLYMLPYAFVVGALAVKLIPAKSFAVFWSAVVLILLTPLTWAPTLRGYPDT
                     GSAFLIALAILIYFQDLRLNNWWQPLLIGFCVGLSILFRRHFAYDGIAFFAALCLRTL
                     ISFIQNSGKSLNEAFYNLIKEGLRIGLAVIFMVITLILIGWPFVDRAIHTNFKQLYSA
                     WELPVADVIWYYVTSFGWIAWILAFIGYVSGIYFRVLVRPYAIFIFLFGTTSMLLWFF
                     YLKIVGIHYTLHITTVVLLGFFSLIWTMWERFKGPTKIFALSAIGIYLIYNAIVGLAN
                     VDILNNTPLQPTRIAHNLIPEIKGTKLSELFSANYPPLHRRDYGEIIRLVSYLRSIAA
                     PDDPIYVASVTELFSKFHLENAEKKLYNSDLRLNVLDVPNTTSRDYYPLEKLLISNYM
                     VLGIPASGGIKVVNVIAEAFTKRWEISKDFVLLPKQFYLYNGHSLTQKVVIYKRINET
                     SLKTALDTLNKMQDYIGVRPGGQLSWLNLNNRTGIYVKRNKDSTYDIEINSLKREQNP
                     TLTFVYIDGLKNQAFISGKFYPESGDCNDISLSVSAIDSDGNVKSRTQTLQLIDVGPK
                     FMPSLKTINSHYLLFDINVGDKKSRSANNCSLSIRNITVSSNQFSY"
     gene            15193..15720
                     /locus_tag="VI935_04295"
     CDS             15193..15720
                     /locus_tag="VI935_04295"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLE24854.1"
                     /translation="MIPEDHICYLVESIVETLDFTTFDKNYSGAGHPAYHPRIPLKIL
                     IMGVLDRVRSSRRLAKNARENVVYIYLSEKLTPDFRTISDFRKDNSDIVKEALRHTVT
                     LAREEGLLDVTHLSTDGTKKRTNASNRRVGQVSFLVGRCKRRQNILKQPSFRIPFQAL
                     YASSSSWLSQVLNEA"
     gene            15747..16154
                     /locus_tag="VI935_04300"
                     /pseudo
     CDS             15747..16154
                     /locus_tag="VI935_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002745248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
     gene            16183..16431
                     /locus_tag="VI935_04305"
     CDS             16183..16431
                     /locus_tag="VI935_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24855.1"
                     /translation="MLPFNTKWGLGWGGGNYYGKGIDAVEKDLLAGTPLTALAERHRD
                     FLIHSLNKDELAKLMQMLHDAGIGPFAQMIGDQSKQGY"
     gene            16627..17415
                     /locus_tag="VI935_04310"
     CDS             16627..17415
                     /locus_tag="VI935_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008178603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLE24856.1"
                     /translation="MIIPEFSPSRRLIYIFDLLFELVARDMKLRYKRSVLGIAWSLLN
                     PLFQLLVLLFIFNMVVPVNIPNYFSFLFTGLLSWIWFQSSLLLATGAIVENRDLIKKP
                     GLPAAILPTVTVTSSLIHFLIAIPILLIFLKLDGIRLTSASLALPIVIAIQFILTLSL
                     AFLLATLQVFFRDTLYLLGVFLNLLFFLIPIFYDASKVPERFKTLYNLNPMVHIIEAY
                     RTILIRGELPDNLSLLLVGLLATGLLIFSYKIFKRASYYFAEEL"
     gene            17416..18699
                     /locus_tag="VI935_04315"
     CDS             17416..18699
                     /locus_tag="VI935_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008178601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLE24857.1"
                     /translation="MSYAIVVNGLSKKFRRYHPDRPLTLQEAILGGLGRIKPVEYLWA
                     LRDISFKIESGRTVGVFGPNGAGKSTLLRLISGVGKPDAGSVVVRGRIGALLDLGAGF
                     HPDLTGRENVFVVGVISGLTRSEVSERFDSIVAFAELEEFIDSPVRTYSTGMLMRLAF
                     SVAVHVEPEILLIDEVLAVGDIMFQRKCLEQITKFKAKGCTIILVTHDTAQIRNLCDE
                     AIWLHRGRVVARGPTNAVVDQYIAEGLAETKRRTPAEWPVAHTDAGTELRINENRFGS
                     LELEITAVKLLNGKGLPTNELKSGDQLCVVIAYNASQPIPGPIFFININREDGLLCYD
                     TTTSGAGLNLPTIQGNGQVKLYIERLDLTRGLYYVNVGVCERDWAYSYDYHQRVYPLV
                     ISSDGGEKGIIHPPHRWEIEGEQKLQTTVPIRKAP"
     gene            19222..20811
                     /locus_tag="VI935_04320"
     CDS             19222..20811
                     /locus_tag="VI935_04320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE24858.1"
                     /translation="MEQKGKTVQIERVLKWWDYPLFILLTAILIFAIYTFISYWLSLK
                     DWLYYPATFVMITLMLLIILGNSLLRWLLLLYIRRPKPMIPSPSLKVGVATTFVPSAE
                     PLDMLEDTLRALIALDYPHDTWVLDEGDNDKVKVLCQRLGAYHFSRKNLKHYQMETGP
                     FQSHSKHGNYNAWLYETGFKQYEIISIFDPDQVPEAAFLSNVLGYFEDPKVSYVQVAQ
                     AYYNQKASFIARGAAEESYGFYSSIQMASYGIGQPSIIGCHSTHRVAALKEVGGLAPH
                     DADDILLTLLYRGHGWKGVYVPKILARGLAPVDWNGYLTQQLRWARSGLDIAFRIHSQ
                     LSMNLPLTKRIISVLHGLNYLYKSFLIFTGLVILVFMLATGITPKVVSFSTLPRLAIL
                     CLAIQICGFYRQRFYLDWRAELGIHWRAVLLHYAKWPYMFSAFFDVILNRRVPYALTS
                     KVKERPRYQTLLWPHILVVIFIGISMFVGLVSGHNINPFLLICAAIIVIISLALILTE
                     LLNFPDPYDRKLRNYLNIKHV"
     gene            20935..21171
                     /locus_tag="VI935_04325"
     CDS             20935..21171
                     /locus_tag="VI935_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24859.1"
                     /translation="MSSSVKALKDILLSAYKLSRWLQAEGYCGVVGFDFGEYFNPGTG
                     EFKHFLAEVNPRISSPTYPKSLMEHLNREQGHKG"
     gene            21583..22344
                     /locus_tag="VI935_04330"
     CDS             21583..22344
                     /locus_tag="VI935_04330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLE24860.1"
                     /translation="MSEQFSKFKEKFDPEEWSDIYKSSKRNNKAFIFKHGSELVEDTC
                     SKISRPNELWLDIGCGTGDLAVKLSEMGLSVIGVDHDTRMIEFANKRFLWHSIVNRLT
                     FTTAKAEFLPFFDNTTDGIVAVSLAGCLSSPDKFFQEAYRVLRKNGFAIITFTNRASL
                     LLKINWCLNKTLHMIRKPTDSDDQYQIYACTEVADKLQKIGFRILNTRFYNFFLNAGD
                     RMIPPEWLAIHCEHFNKYKMGHRVGGNFIIVAQKI"
     gene            22374..23231
                     /locus_tag="VI935_04335"
     CDS             22374..23231
                     /locus_tag="VI935_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLE24861.1"
                     /translation="MNKVSFSLLRGVCQMPAYVAYEKGFFKEEGLNVNLNIEPTAWLV
                     PHKLVSGESHFAVIPWTRVAAAEDKDTPLVLICGSGFEEAVIVVRKEISTSKVRKVAV
                     PLRGGMKDLTAMALIEGLGWKDVELIRLPSGDGAIISLFGRGADAASMVEPYATMMEA
                     LGIGTVVKRTGDVWKGAPGCSLTTTMELKETSPDLTQKVVRAFVRGVTFVKENPDESA
                     QIASRYICINAGFIRKALNVNKPDVEAIRNKKAMEEILSLMMKLGYIKRLPEGYVDLT
                     FLDKAMKRI"
     gene            23290..23436
                     /locus_tag="VI935_04340"
     CDS             23290..23436
                     /locus_tag="VI935_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24862.1"
                     /translation="MEGSLFQSFMKIGLTSRSIMRRREGLRVWLGLDSSERSKIEGKR
                     DTKR"
     gene            24052..25659
                     /locus_tag="VI935_04345"
     CDS             24052..25659
                     /locus_tag="VI935_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLE24863.1"
                     /translation="MNEELKLLKRALNNDLILFFSVFVIGVLLRFRHLTSGLVESHSQ
                     RQADLAMIASNFYSNGFDIFHPQIDWAGSFPGYIGMEFPLVPYLMALLYALFGKHLFL
                     GRLLSISFCALSTYYFYSLVRSYFTKRIALISLVFFLFSPVSIFFSRTLISDNAMLCF
                     SIGAIYFFSQWTKNEKTLYFILSLIFAMLALLVKIPAAIIGLPIAYLAWYKYGWSFLR
                     MTRLWIFGFIVTIPSILWYVHAFTLAKNNYPFIMFGEGRWVWYSGFEIFLDVAFLKKV
                     FVRLTAFLITPIGLIMSLAGAITTAWNKPNTYLFHWWAAGFFVFFIIGFPGQGHEYYQ
                     LQLLPVASPFAALFCDELMKYRGAYNSGILKPGLRTVIVICLIVGFLVSSLAIVETRY
                     HSSIEKILYEGGLSVQSIVPVNALILAADWDNPVLIHASKRRGWHFPGSKTYKDLVRT
                     WSVSLLEKMRSQGASYFVAPIKLGMNRQIASENFLQSVGRRILPPRDVDFLESHPAFK
                     RYLDSHYKKVLSNRFVVVYDMRTKANM"
     gene            26035..27027
                     /locus_tag="VI935_04350"
     CDS             26035..27027
                     /locus_tag="VI935_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24864.1"
                     /translation="MLSKMLDTAMKPIARLLQYNYDAKPMKPLGVNNTMKIARAYNRF
                     KYGRYHEYRKSQYLHHFDKLIRENGLPFSPLNEIKDGWALDTSKTLPHLKQLLADAEE
                     IIKERAGVKRTDFGRPYFQEIPFDDLLVKYPSILNFATSSDVLSTICNYLGLIPVFSA
                     ALPPGIRFNETWAKFEDVPDGPPKSSQLFHLDYHDSPMAYALVLLRDATMENGPFCIL
                     PESVSKRASEALNYRARGRRHRVTDDEMYSVVPESELIKMCYPAGTVMFLDNSRCFHY
                     GSRNAVKPRYLLMLAYISPCRTDFGEVLLERKIYPVRDNDSRLRKMVLNREFME"
     gene            27037..28614
                     /locus_tag="VI935_04355"
     CDS             27037..28614
                     /locus_tag="VI935_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24865.1"
                     /translation="MLNDLTMFARYARGLNSYLRHTLSPEQCRQMLVNHLESRDESFL
                     HILERGIYTNPRSPYRKLLLHAGVEFADVVSLVRRHGVEGALARLYDAGVYVTLNEFK
                     GRRPIERPGLQMGVRPRDFDNPLLVKYYEARTGGSRGVGTRIIIDLDLLTHEAAYLHY
                     FLAAFDLEETSIGAWHEVPPVAAGMKLVLRYVKLGKSVERWFAQSRLGLRPGDLKFFI
                     FTKYTIYASGILGKQLPVPEYVPLEEASKVAHWLAIKKGNGKPATLDTNPSSGVRVCL
                     AAKEEGLDIAGSLFRVGGEPYTPAKAQVVAEAGIRAVCHYSMSEIGTIGTACTSPADL
                     DDVHLLTDKVAVIQRPKSVGTGELSVGALVYTTLLPSCPKLMLNMESDDYGVLENRVC
                     SCPIGELGFTKHLSRIRSYEKLTSDGVTFLGTELLRLVEEVLPTRFGGYPTDYQLVEE
                     EEEGLPKVNILVSPRVGDVNEEDLLSTAFQVLHSYPGGDIMSEKWRQGQTLRVARREP
                     YTTASAKILPLHILQKK"
     gene            28643..29803
                     /locus_tag="VI935_04360"
     CDS             28643..29803
                     /locus_tag="VI935_04360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE24866.1"
                     /translation="MKILFLIQSLEIGGVGRQLSILTNHLALRGHDMSVIALYATDQN
                     WKLIWKLDSIDIRSLLLNKPTGVLAAGIELIKATLELRSLLKRENIQILYGYLGHTAR
                     FIAWLATRGMPNTKLIWGTRTSGDNTDLYPRNWKVSFLFNLCKWGSASVPLMISNSEA
                     GYAYRKAKGYGCQKHLVITNGFDVEKFKPDPQARVRMRSELGALENEKLIGRVGRLDP
                     MKGYPDFLEAAALLAKERKDVRFVIAGDGPDTYRRQLQLLSQELGLTERLIWAGVRED
                     MPAVYNALDIACSSSSYGEGFPNTIGEAMACGIPCVVTDVGDSAKIVGDEGIIVPSGD
                     PKKLANGLKTMLLQLDDIKPHLLRERIMNRFSIETMVESTEKALIEVCNTSK"
     gene            29843..31000
                     /locus_tag="VI935_04365"
     CDS             29843..31000
                     /locus_tag="VI935_04365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE24867.1"
                     /translation="MKILFFIQSLGMGGAVRQLSILSDHLVRRGHDVSIVALYTTDQN
                     WNLVWKIDSIQVKSFLLQPSTGALYTGLQLIKTAVQLRNMIKREEIQVLYSYQGDIAR
                     FIAWLATRGMPNTKLIWGIQGSGQRNTLSGNNWKVSLPFYLCKLVSASVPLMISGSQA
                     GYNSRKAKGYSCQKQIVILYGIDIDQFQPDPQARVQVRSEWGALENEKLIGRIGRLAP
                     DKGYPNFLEAAALLAKERKDVRFVIVGDRSDSYRRQLQLLSQELGMTERLIWAGARED
                     MPAVYNALDIVCSSSYSEGFGLTVAEAMGCGVPCVVTDVGDSARIVGEEGIVVPPGDP
                     LKLADGLKTMLLQLDDIKPHLLRERIMNRFSIETMVESTEKALIEVCSAFK"
     gene            31020..31781
                     /locus_tag="VI935_04370"
     CDS             31020..31781
                     /locus_tag="VI935_04370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLE24868.1"
                     /translation="MASSIPILTFHAVDGRSSVISLSPRVFRRGMATLHENGYQTLSL
                     PEAVNFMRQGKPFPDRSFVITFDDGYQTVYDEAIPILKGYGMSATVFLTVGEKSASNP
                     ADRLPSLNGRSMLNWREIREMKQWGFDFGSHTLTHPDLTRLPLERVEIEIHNSKAIIE
                     DALSDLINCFAYPYGRYDNRSREIVKKHFACACSDTLGLVNQGSDLYALKRIDAYYVR
                     TDGLFNLMLTKFFPWYIKARNIPRRIRRAILLGLS"
     gene            31781..33034
                     /locus_tag="VI935_04375"
     CDS             31781..33034
                     /locus_tag="VI935_04375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLE24869.1"
                     /translation="MNTPLRFCMITTFYPPYNFGGDGIFVRQLSNELAKRGHHVDVIH
                     CIDSYRLLARQEPTKTYDDHPNVTVHGLKSRLGFLSPLTTQQTGYPFFKSASIRRILE
                     KGFDVIHYHNISLVGGPKILEYGQGIKLYTMHEYWLICPTHVLYRFNRAPCTRPHCFL
                     CSLTYKRPPQWWRYFGLLETTVKNVDAFITLNHFSKDLHYQMGLNVPIVNLPPFVPTA
                     EGVLSTSEKQFEETLLEPYFLFVGRLEKLKGLQTLIPIFRHYRKAQLLIAGTGNYEPE
                     LRQLANDSTNIRFLGFLPQQQLQLLYQQAAAVIVPSLCFEIIPLVIIEALREKTPVIA
                     KNLGGMPEIIEESGGGFVYDTDEELVAAMDRLLAEPSYRRELGLRGYQVYQQKWTPEA
                     HLKLYFALIHEISSAKRLRISQPNA"
     gene            33363..33638
                     /locus_tag="VI935_04380"
     CDS             33363..33638
                     /locus_tag="VI935_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HLE24870.1"
                     /translation="MRKNSRSEVEEKVKHILISQLEIRPGILAESSSTTPLLGRGIGL
                     DSMEVMALVAALETEFDIEIPDEDLTTELVKDIGNLAEYLLQKISKG"
     gene            33793..34143
                     /locus_tag="VI935_04385"
     CDS             33793..34143
                     /locus_tag="VI935_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24871.1"
                     /translation="MLPRAVLLVPEPESTPTALGIIRSLGRVSIPVVAAASNLTTPSF
                     FSRYVKHRLVCPALEFEPINWKDALIEFGKSVDEKPVRWVSLFDGKPRVIVSSFINLD
                     KRQFPYEHWRNSET"
BASE COUNT        10032 a         6885 c         7443 g         9807 t
ORIGIN      
        1 ggtaggaagg attcaaaatt ttaagaactg gagcccttat atggttccag atcaagacaa
       61 aacctgcttg ggactagagt atttttgctt tgaaggtgac gggctctgga caatgtctaa
      121 tcaagagtta atcgagcttg gtaagaagga attggagata ttaggattgg ttcaaccctc
      181 tgaggttgag gatggaactg ttgttaggat gccaaaggcg tatcctgttt atgattcaac
      241 ttatagagaa tcacttcagg ttatacggca atttcttgac caatttcaga acctgcagct
      301 cataggccga aatggtatgc ataaatacaa taatcaggat cactccatgt tgactgcaat
      361 gttagctgta aaaaacatct taggcgcgaa ttacgaccta tggcaggtta acgccgatca
      421 ggaatatcac gaagagatca gggaaggtga gggaaaggag ctcgttgatt atgaacacct
      481 tgcttctacg cagcctcagg tacctgagag gagactgcca ccttcttacg atgaggtcat
      541 tataaaggcc tttgccagaa tggataaatt tgcccttgct accgcgctgg gttctgtttg
      601 cggcctagcc atttttattg caaccctttt gttgatcatg aagggtggta aagtcactga
      661 tcttaatctc caactactca gacattattt tatcgggtac accgttacag tgaagggagc
      721 ctttatagga atgggttata gtttcttctg gggatttgtt tggggctggt tagctgcata
      781 cctcaggaac ctttttctcg gctacttcat acatagagct aagagtaaag cagaatcctc
      841 atcgtttaga aacttgcttg attatatatg atttgatttt tgcattaagg aaatgagaag
      901 tgactaataa taacctatta gaggaagaga agctcttaag tgccgttgtg cggttaaatg
      961 ccaaaatgtt ggggttaatc cttggcctcc tttttggatt gcttatcttc ctcgcaacca
     1021 actggcttat aattaaggga gggccaataa cacgtagcgg gaaggagatc gtaggtccac
     1081 atttgcaact cctcggtcag ttttttattg gatacagtgt ttcattcctc ggaagtatta
     1141 ttgggtttct ctatggtttt gctatcggta gcatatgtgg aacatttatc ggctggatct
     1201 ataattgggt tgttgattta aagaaaggat aaattttact aagtaaggga gtacttagaa
     1261 taaaacatgc caatctatat cgtcttacaa gctccctcag gcctatggag acataattag
     1321 ggtttcaagt tttcaaatta cataaagtag ttgtttatcc aagcctcaac caaaattata
     1381 aaagctacca gatggttaat cgcatcaaaa acataactac aatgtattgc tcagtcacaa
     1441 ttgtccttca tcaacgttaa taacaactga ataggactgc aatatgctat ggtgggtgaa
     1501 tttatctggc acatcgagta tcggttcaaa tattactacc tagggtagga gttagcggat
     1561 ctattcaggt aatggagtta taatgcgtag aattctttat gcatatccag aggtttcgcc
     1621 gatttttcca aatggtggga taggaacgtt tgtatttgag gtatcccatc ttctggctgc
     1681 tagtggtgaa tgggatgttg atatcctaac agataccagt tactcggcaa ctattatgca
     1741 agatgatttt cggaaggcag aaacagtatt caaacaggcc ggcgtccgtc taatagatct
     1801 ttatcgcgat gagaggatta agccggggtg ggaatctccg gacgtgattc gtgcagagcg
     1861 gtatcatgct cacgtcacca aattgcactc tgaaaggcat tatcacctta tcgaattccc
     1921 ggactggcgt gcgccaggtt tttttgtagt tcgtcacaaa aaaaccacaa agtcttttgg
     1981 caatactctc ttagtggttc gtctccattc ttcaacaaaa gatgtctggg attggcaaga
     2041 tgggtatttt tcaaataaaa atgacctgta ttgtcacttt atggaagagt atgttaagaa
     2101 gtattcagat attgttatga gcccaactga gttccttttg agacccgttc gtcaaaccca
     2161 gcttgtcttt gaaaaactgc tatttcgcga cggttatcca atatcttcga attaccagcc
     2221 acaccagaaa gatatacacc acgatcgtag atctgatccg atcacggttg catgtgttag
     2281 tcgtttagag ctcagaaagg ggcaggatat acttgcaaat gcgataagac acctttcaga
     2341 atcaaattct ctcgataaca atgtgcagtt cgttttctgt ggcaaagata atatgggact
     2401 tgttcgtgag cggtggatgt cgcagtctat aaagcaaatt ctcaagggag tcaagaactg
     2461 gaagatcatt aaacccaaga gccgtcaaga cctcatgaac tggttagcca ccgaggttga
     2521 catctgcgta gttccgtcga gggttgacaa ttatccaaat gtagtaatgg aagcttctgg
     2581 cgctgggtgt tatgttatcg ccagcgactc tggtggaatc ccagagattt ttgaagatta
     2641 tgagattgca ggagctttgt tcccgccggg agacattctt gctctagagc gggaaatacg
     2701 caaggcgatc caatatttga aaagcaatcc aggaatccgt gtacatattg ctgaagaatt
     2761 tgagaagagc cggcagaaga aagctagtca aacaatcgct aaatatgatg aaattatcga
     2821 gttagggaaa aaacaccttg cctcttccac aattgaaagt gcaacgcctt gtgtaagcat
     2881 cattatccct ttttataacg cccataaata tgctagggat gcaatagaat cagcctttac
     2941 atcggagtac ccagattttg aggtcatcct aattaacgac ggatctgaca atccgaagtc
     3001 cctcgaattt cttaaggaga ttgaatcaga attcccaaaa ctcaaaatta ttcataagtc
     3061 aaagggaggt ttgggtgatg caagaaacgc tgggctagaa gctgcaacgg gtgaattcat
     3121 tgtacccctt gacactgaca atatgctttt acccaatatg ttgagttcat gcagccgtgt
     3181 ccttacagaa catcaaaact tagcgtacat tacaacttac tttgaatgcc taagagaaga
     3241 agacggggct cggttgccat tgcagctatc gccgatccct agaccgttgg gaaccgtcga
     3301 tggactaatt ctcctcgagc ccacgcctgg ctatgcccct gctatgcttc gtctaagtgc
     3361 cttgaaatct gtcggaggtt acgcctctgg tatctattgc atggaggatt gggacctatg
     3421 gttgacgttt catgaaaagg gaatggaagg ggacgttctt ccagaagtcc attatattca
     3481 ccgattacga tctgatggta tatccaattc tctcaatgcc ttaaacgata ttcgactaaa
     3541 acagtcttta ttgcgaaagc atgagtgttt ggtaaaagag tattctctag ctataactac
     3601 cttgttatta aatgaataca ggaatgggtg gagaggacag gacgaagatt ggattgttct
     3661 aaaggacatg tttcgacgtg ccatgaatga tccagtccgc gcacttaaat atgtgacaat
     3721 gaaaataaag cacagcctta aagaacgggt tagaaacttt agtcctatca aggcactcat
     3781 gaaatctcaa gaatagcccc taggttttta ccctcttttt aattcgtaaa tcattatcgt
     3841 ctcgctatac tgaaatgaac agcctgtcac gattagtaca gaatcataag atgaatgaca
     3901 aagccaagaa tcacattgtt atagtggagg cttacgaagg tttcatcgaa ttcatttctg
     3961 atgaaccgga ggagcggatg gctgcgttag gactcggcct cccactcgaa gaaattgctg
     4021 aggatcttga ctacaggatt acaattcttt cctgggatga atggtaccaa tatgaagtaa
     4081 aaaagacttg ggtgtcgact ccagcgacac gcccagcatc aaaacccgat ttcggaattc
     4141 ctttaaaggt cttcaaatct aataagataa aacggattac ggttcccgca agagaccctg
     4201 agcataggct tccattcgac ataaaagact tagaccctcg atcctacgga cttatttatg
     4261 acagtggttt actacatttt gtttcttact gcctttcaaa agaacttcaa gacttttacc
     4321 gtaataaccc tttccaagct gtcatcctac cgatgtgggg cgggctcgga tatgtttcac
     4381 agatggcaaa gtcaactgaa gtccccaact gtatcagtgt gccttttgca gttgtggtta
     4441 cggatagatc agccacccgt cagctagcga atcaagaagg aatatggaca agacaggcaa
     4501 tcgttaggcg ccagatggaa gatgtttcac tcgctctagc cgaccttgtt ctggtcttcg
     4561 gtccaagggg agcggatata gccaatgccg ggaggctacc tgaggcttcc cctcctgttt
     4621 atgcccctcg ttttgtagag cgattacttc tagataaaat cgctaacgaa tctatgcaat
     4681 atagagatgt tggtaagccc gttcagttct ttctctacga gcctcaagaa gccgcctcgg
     4741 gagtacttac cactcttgat gctgtaactc ttctaagaaa taaaggggta cgtcttgatc
     4801 tgcccttgat aaccgccgga cctccaatgg tctttgctcc catgaaacca agaggttttg
     4861 tagactactg gtcctcccgc ggagccgtgc aagagcttgt tcgtgaacgt caatgggaat
     4921 ggaggagaga ataccccgat tttaaccagg tctttcccgt tcgactctat ccaagctatt
     4981 tcgagcatct tcctaatgtt tggactgaac ttgcaagagg ttcgttggtg cttctttcat
     5041 cagccgctgc cgaggggtta gcgcccggac aaattctccc ccaggaggtc ataattgaag
     5101 gtgatcccgt gccagataag ctagccgact gcattgaaag aatttcccga actgaggtga
     5161 aaaaactaga catggtcagg cgcgagctat gcatgcgagt tgccgctgca aacagtagag
     5221 tgagacaaag gcaactcctc aaggaaacta ccaatgcttt agaacgactc attcaatttg
     5281 cccccgaacc ccaggatcta tcacgagttg ctcttatgtt tttcgaccgc cgcataccac
     5341 ttaaacaaat agcacagaat gataaaacac cccccttgcc agaactgagg ccgggtatca
     5401 ataaagggac actatccgtt gctgtaacct gctatgaaat gaagtccatg attagggaag
     5461 ctgtagaaag tgtctgggcc tcagagcgcc agcctgacga agtcttactt atcgacgacg
     5521 gctcacacgg agaggaaact ctcgcgaaca ttaggaaact tgaaaagaaa gcatctgaaa
     5581 gaggccttcc tttgagagtt attcggcagc gaaacaaggg gttggctggg gctagaaacg
     5641 ctggactcga ggcagcaaag ggagaattca tctccttcct cgacggggac gatatgatcg
     5701 aacctcagtt ttaccggctt gctatgcgga tcttcacgag gtatccacgt ttgggtggag
     5761 ttgccgcctg ggcttttatc ttcggggaag ctacccccga tggtttttgg aacgctccgc
     5821 aggcagaact accttttctg tttatcgagg atagtgtttt cgttccctgc gtgacacgtg
     5881 ccgaactcct gcggaatatc ggtggctatg atactcgtct gcggtataac tatgaggatt
     5941 gggaattggc tattcgaatg cttgtctcag gctgtcctat tgtcacgata cctatgcacc
     6001 ttttaaagta caggatacga cggggttcgc tcttccgcag tatgacccat gtgcaaaatc
     6061 aggttatgcg agagcttttg ttgacaaatc atcgtgatac agtttccaaa tttgctgttg
     6121 aaatcgccat gcagttagag aatctgtgga tgaagcaacg ttatccagac aaccaaagag
     6181 acgctctctt gaattacaat tctagaccta atgatgaaat atttatcccg caaaagttgt
     6241 acagcctgta ccgcttgctc cttccgaaac ccttcaagat tattttgcgg aagactcaac
     6301 ggattcttaa cagtatatca gcagattaaa cagaggctta tcttttatga agcaccctat
     6361 actctgttcc tgatttcaaa tatgacttct cgtgtgacaa actaagataa aagagcttaa
     6421 aaaaaatgag tattatcata tcagctataa tatgtacaca taaccgtgct gagtacttaa
     6481 ctaaagcaat ccaaagcttg gtaggtcaga gcatatcaaa agatagatac gaaataatag
     6541 ttgtagacaa ctgttcaacg gattttacaa aagaagtagt tgaacggttc tcaagcaaga
     6601 gtgacataag atacatatat gaacccactt taggtgctaa ctatgcacgg aataccggct
     6661 ggcgcaatgc aaggggaaag tatgtcgctt acctggatga tgataccgtt gcttgtcata
     6721 tctggcttga taagatttta gaagtgtttg aaactgtaac gcctagacca ggctgcatag
     6781 gagggaaggt agagccaata tgggagattg ctagacccaa atggttatcg gattggctct
     6841 tacatggcct tgctcttata gactggtccg aaactcctcg tgttttgagt aacatatctg
     6901 tggaatggct tgttagtgct aatatagcat ttccagttga ggtgctcgaa cgctttggtg
     6961 gatttcattc aggtcttggc aggttaggaa agaatctttt gtctggggac gaaatacttt
     7021 tggaaaaaca aattttgaag tctggttatg cttgtttctt ccaccctgag atcgctgtta
     7081 gacatcatat accgaaatca agacttaatc aggattggtt tattcgtaga tatttctggc
     7141 aaggtgtcac cgacgccaca atgcaattac ttgaggaggc cccatcaaca acgaagcgca
     7201 tactcattgc tatgtcaaag acattaggtt taatactaac tccaagaaag ttagtgaatc
     7261 tcataatgcc tgtgaacgat ccagggcgat tcacagataa gtgcttcgca ttaataacac
     7321 ttggacatat attgggttta ttaggctctc ctacaaaatc aagaagactt aaaaataatt
     7381 caccttaaga taaacaatag ccttagagta attatctcac ttaaaaagca taatgactta
     7441 taattgaaga agataatgac ggattaaaga aaaggtttaa agacacagcc taggagttat
     7501 tctttaaata ttaattgctg cctaagatta cgagatggcg ataaatatac acttaatccg
     7561 tacccattat cctcattggg gtcagtactc tggtataaat cagttcttga aatatataaa
     7621 tcaaaatagt tatcatgttg acctccatct ttcaaatgat aatgatgagg attttcctat
     7681 acgaaacagg ttcgtacaaa gatgccttcg ttattttgtt agacgaggaa tgcagcaata
     7741 caagctgagt gacctgactg ctgaaattaa agcgtttcga aaatgctttc aaaataaagt
     7801 ggacatagta cattatcttg atggtgagca ttcggctcat tatctaccat ggctatttaa
     7861 aaaatggaat aaattcacaa ctaaaatttt agccacattt caccaacccc ctgaccttct
     7921 tgactcactt attatcaaag acgtcgttgc taagctggat ttcgtcaccg ttttatctcc
     7981 agatcaagtt gcctacttcg gtgaattgct agctccagat aagatttgct tgataccgca
     8041 tggcgtagac accaactttt ttaaaccgag aatcaaaccg aaggaggaaa gcaaattcaa
     8101 atgcatcacc gtcggtcatt ggcttagaaa ctttaaggct atgcgagagg ttgccaataa
     8161 attggtatct cataaaaata ttgagtttca tgttgtggca tccagactac atggaccaag
     8221 gcttattgga cttgaagatc taaagaatgt cacgatttat agagataata ttgacgatac
     8281 taggttactg gaattatacc aagaatcaga tgtcttattt ctgccactct tgcagtctgc
     8341 agcgaataat gcactccttg agggggttgc ttgtgggttg ccagtgatat ccactctctt
     8401 gccatcagtt aaattttatc tcccgggtaa ggaagctata ctaatcaaag atagtgatac
     8461 aaaaaaattc gtggatgcca tattatactt agttgataat tctcaggacc gtatagatat
     8521 ggggcgagag gctcggaagc gagcagagga tcttgattgg cgtaacatca ccccacaata
     8581 tgaaaatttg tattcgaaat tgataaccta ataatatatt atatctctgt aaaaccccat
     8641 ttttgagtat gtagaattag agattatgcc gaaaaataac cggctttttt gttccagacc
     8701 attcgaatgg tttgaagtca ctaacctgaa cggtagagga gaagcatatc tctgttgtcc
     8761 ttcttggctt gacacaccta tagggaatct taaagatcaa tcagctgaag agatatggaa
     8821 cggcaaaaag gcacaagaga taaggcactc gattctggat gggtcattta aatattgcaa
     8881 ccgctcaaga tgcccttttc tgcaaaccat atctgggccg gtcgaaaagg tacaagacgt
     8941 taaagatgag aattttaaag aagtgataga aaaaaaccta acaattttat cttacggccc
     9001 taaaaaaatt aattgtagct atgaccaatc ttgcaacata tcctgcccat catgccggac
     9061 agaaataatt atagaaagta aaaataagca acagataatg aaaattcaaa ataaaattca
     9121 gaatgaagcc cttaaggatg ccgatttctt gtacattaca ggttcaggtg acccttttgg
     9181 aagtccattt tttagaaaat ggctccaaac catgaaaaaa gaggatatgc caaatcttaa
     9241 ggatatacat ctacatacaa atgggctgtt atggacaccc aaaatgtggg gtacaatatc
     9301 aaaagatata caagagctaa taaccagcac agaaatctca atcgatgctg cgacttcaga
     9361 aacttattcg ataaatcgta gaggtggaat ttatgaaaag cttcttgaaa accttgagtt
     9421 tattagcacg ctacgcaaaa acggtcccct gaagtttgta acaataagca tggttgttca
     9481 agaaaacaat tttagggaga tgcccgattt cgtacgcttg ggcaagcatt ttaattttga
     9541 tactgtttac tttagcaaaa taagcaattg gggaaccttc tccgaaaagg aatttaggaa
     9601 tcgggcggtt tgttctccca atcacccaaa atattcagag ttagtagatt tacttaaaaa
     9661 cgaaattttt aaagaacctg tcgtttattt aggaaattta tcagaaatcg caaattcagc
     9721 taaaggtagt tttgtacctg cactcaaaaa ccgtatattt acatttcttg caaattaatt
     9781 cagcccacac ataacctaat agcgttaccc caaggaacct aagtaaaatg gacgtatctg
     9841 ttatcatacc agcatataat gcagaagaga ctatttccga tacactagag tcccttattt
     9901 cacaaaccta cccaaactgg gaggctattg tcgtagatga cggctccacc gatgcaactt
     9961 ccgaaattac cctaggcttc ataggacgag atgctcgcat tcgcatgata cagcaaccaa
    10021 acggtggtga aagcgccgca agaaactccg gtatcgccct tgctcgatac gaatggctgc
    10081 tttttctcga cgctgatgat ttgattttac cctcacacct cgaacacctt actaatgaac
    10141 tcctttcgaa ccctgagctc gacgcagtca tctgcggctc agcccgcgta gcgctcgatg
    10201 gaacccttgt tatagaaaat tacatgcctc cggtgaatga tatgttttcc actttagcac
    10261 gacgcgctgc ctttcccata aacgcttgca tcgtccgcag gtcgcttata gagactgttg
    10321 ggaaattcga tacatcactc aagaccgggg ctgactggga tttctggcaa cgtatcgctc
    10381 gcaaaggcgt gtgttttggc gtagtcaatg aggttctttc actttaccgc atgagaccaa
    10441 attctgccac atctaacgct taccaattat tcaaagatgg actacgtcta ctgaaacagg
    10501 gacactctcc agaccccagg gtaaccgccc cacatccaga ttatgtcaat ggattatcac
    10561 cagagggtgt caatagtcaa gtgttttact tactaacctg gtgtgctggt ctattgttag
    10621 gtcgtggcga ggacccccgt ccattacttg agacaattaa agaacataaa tacccagagc
    10681 tttatcccga tgcaatagca aaaattattt ttgaatcaac tatactcccc aaatgtcggc
    10741 ccacacacac atgggaagag ctatggtcca gtatcaaaca acatttagac gagttcctag
    10801 tagcgctaga agcgcagtca cttacaccag acctggctcg ccgcgcccat attgaactga
    10861 aaaaattgat tctgaagcat tcgccgacct ggcagtatgc cattgaggag tatgagcagg
    10921 ccatagctat attggaagaa cagcgaagaa gctggcaggg tcttgctgag gaacgtgagt
    10981 ctaagataat gaaattacag gagaaatttt gggttcgtct gggattgcga ttagaaagag
    11041 ctctgaagca acagattctc aaaagtgaaa aatcctagaa gaggatttga gagccatatt
    11101 ccctcagtaa tgcgactcaa actcaactcg ctctgatttc ttactctaag gggaatttat
    11161 aaaaacgaca catccagtta ttaggttcta tcttagtctg gtaaaagcag tgatcaaaaa
    11221 ggcgataccc gttctgcacg ggtccgcggt ggaagaattt aaggggagag aacagaaccc
    11281 ggcatggacc agaatacgag gtaaaactca cggtcgagga ggggaaaccc aattgtgagc
    11341 agttaattag tcagtgggat tacaaaaata aaggccagtt ttcttatgga ggcgacacta
    11401 cctacaaaaa aggcatggca ttcttagatg gtcacggcac tatagaagat tgggggtgtg
    11461 ggactgctta tgcaaaaaat tttgtgaaga aaagcaaata catagggata gacggaagcc
    11521 agagtacttt tacaaataag gttgttgact taagagaata cgcttcaaaa acggattgca
    11581 tatttatgag acatgtacta gaacacaact acggctggag gaagattctt gccaatgcag
    11641 tatactcatt tacaaagaga atggtgttga taatatttac accatttact gacgtaaccc
    11701 agcaaatcgc cactaactat tgtcccaatg taccagatat ttcgttcaaa aaggacgact
    11761 taacagaatt cttcaaacag tttaagtaca ctgaggaatc gcttgagact gaaactcagt
    11821 acaaaaccga gcacattttc tatatcgaaa aataaaaaat gtccttccgc caatgcaagg
    11881 tcaattcttc ggagcactat cgcagtgtta tgaaaccata cgaaaaactg aattaggttg
    11941 ttataatcag ctcgcttgat aataaagtct taagagaagt gtagagtcaa tattctctag
    12001 cgggctaaaa tgagattaac aacagtttat ttttaagtgt gaatataaat taatggatat
    12061 tattttagtt tcgggctcgg caggtttaat tggttctgaa tcagtgcgtt ttttctgcga
    12121 ccgcggttat acagttgtcg gaattgacaa caatatgcgt aagtttttct tcggtaaaga
    12181 tgccagaaaa gttctatata cttagctgct ccaccattag ccccatttat aagcgctgta
    12241 tcgacatgaa tgccatatct tcttagtctt ttttctctcg cattatataa ataatcccaa
    12301 aaacttacac cctttttatt agccctataa atcgactgta ggactgtctt atagtttagc
    12361 cctaacgtat cgcacaaagt atttaaattt tcatcgtaat ccttattttg tatcatgttt
    12421 tgaataactt tcctgacaat agttgggaaa tctctaagat ctgccacttt cttggttaat
    12481 ccttgcataa cgattatatt atatcacccc ttgtaactta attaaataat gacaccattt
    12541 ctatccctct actagaatct aatctaccga tttaacttta taattgacgg ttatcttggg
    12601 tggatttata agtggattta taattggttt taaagtattg agaggctttt cctatatatg
    12661 gtgttttcta gttttcattt cgatgtatca attattggta tctatctacg aagtaggagt
    12721 ttttttgaga cgtggatttg ggttaccgat acctttgaca cacataaaat tcgcaattta
    12781 agatattaaa tcactttttt taaggattac tactaataaa tagaccatag ctcttaaata
    12841 ataatgaata atcctgaaaa aaaatcttcc aaaattcttt ttattaatat tgccctcctc
    12901 ctaatagtga ttttgataat tttaataact gcatctcaat acatttcaag agagaaaact
    12961 atttattatt gggattacgc aaattatgaa aataggacaa aaactatttt agattatccc
    13021 caaggatcag ttaaagagtt tttttttgct gtaccaaaag aaacttggct atcaacgggt
    13081 gaagattata gtaatattcc ctcaattcct ttgctacccg ttatgcttac gttcggtgat
    13141 tcccgcacgg tatttatatt aggtatggct ttgttataca tgcttcccta tgcgtttgtc
    13201 gtaggggcat tagcagttaa acttatacct gctaagtcat ttgctgtttt ttggtccgcg
    13261 gtagtcctta tcctattaac acctctaacc tgggcaccaa cattacgtgg ctatccagat
    13321 acgggatccg cttttctgat agctttagca atacttatat attttcaaga tttgagactt
    13381 aataattggt ggcagccctt acttatagga ttttgtgttg gtttatccat actatttcga
    13441 cgtcacttcg catacgatgg aatcgctttt tttgcagcat tatgtttacg gaccctaatt
    13501 tcttttattc agaattcagg taaatcctta aacgaagcgt tttataatct tattaaagaa
    13561 ggtttgagaa ttgggttagc tgttattttt atggtaataa ctttaatcct aattggttgg
    13621 ccattcgtgg atagagctat acataccaat tttaagcaac tctattccgc ctgggaatta
    13681 ccagttgcag atgtgatttg gtattacgta acatcattcg gatggatagc gtggatactt
    13741 gccttcattg gatatgtatc tggaatttat tttagagtgc ttgtacgtcc ttatgccata
    13801 ttcattttct tatttggcac gacatccatg cttctatggt ttttttattt gaaaatcgta
    13861 ggaattcact acaccttgca tataacaacc gttgttcttc ttggtttttt ttcattaata
    13921 tggacaatgt gggaaaggtt taaggggcct acaaaaattt tcgcattaag cgccataggc
    13981 atttacctta tttataatgc catcgtaggt ctggcgaatg tggatatatt aaacaatacg
    14041 cctttacaac caacaagaat tgctcacaat ctgatcccgg aaattaaggg cacaaaactt
    14101 agtgaattat tctcagctaa ttatccccca ttacatcgaa gagattatgg tgaaattatc
    14161 aggttagtct catatctgcg gtcaatagca gcccccgatg acccaattta tgttgcatca
    14221 gttacagaac tctttagtaa attccatttg gaaaatgccg agaaaaaatt atacaatagt
    14281 gatctcaggt taaatgttct ggatgttcca aataccacct caagagatta ttatccacta
    14341 gaaaaattgt taatatcaaa ttatatggtt cttgggatcc cggcttcagg tggcattaag
    14401 gttgtcaatg taatagctga agcttttaca aaaaggtggg aaatatcaaa ggattttgta
    14461 ttacttccaa aacagtttta tttgtacaat ggtcatagtt taacgcaaaa ggtagttatt
    14521 tataaacgta taaatgaaac ttctcttaaa acagccctag atactttaaa caaaatgcag
    14581 gattatatag gagtacgtcc gggaggacaa ttaagctggc tcaatttaaa taatcgaact
    14641 ggaatttatg ttaagagaaa taaagattcg acttatgata ttgaaatcaa ttcgctaaaa
    14701 agggagcaaa accccacctt aacatttgta tatatcgacg gattaaaaaa ccaagcattt
    14761 atttctggta aattttatcc tgaatctggt gactgtaatg acatatcgtt aagcgtatcc
    14821 gcaattgatt ctgatggcaa tgttaaaagc cgtactcaaa cgttacagct aattgatgta
    14881 ggccccaaat ttatgccatc tctaaaaacc atcaattctc actatcttct attcgatata
    14941 aatgttggcg ataaaaaaag ccgttcggca aataattgtt cattaagcat tcgcaatatc
    15001 actgtatctt ctaatcaatt ttcctactga aaatgaaact ccctacagca agcaggctgt
    15061 ccgacaatta ggatatatag attataataa ctagtatcaa ggaaataaat cctaggagag
    15121 gaggtattag aactagcata tattgaatca tacaaaggac agcagtggtt attaccgccg
    15181 agcatagagg agatgatacc tgaggaccac atctgttatt tggtggagag tatagtagag
    15241 actttggatt ttacaacatt tgacaagaat tattcagggg caggtcatcc agcgtatcac
    15301 cctagaatac cattaaagat actgatcatg ggtgtattgg acagggtaag atcatcaagg
    15361 aggttagcaa agaatgcacg cgagaatgtc gtatacatat atttatctga gaaactgaca
    15421 cctgatttca ggactatcag cgattttagg aaagacaatt ctgatatagt gaaagaagcc
    15481 ttaaggcata cagtaaccct tgctagagag gaggggttac ttgatgttac acacctttca
    15541 acagatggca cgaagaaaag gacgaatgca tcgaacaggc gggtgggcca agtctcgttt
    15601 ttagttggta gatgtaagag gaggcaaaac attcttaagc agccctcctt tcgaattccc
    15661 tttcaagccc tatatgcttc atcctcttct tggctttctc aagtcttgaa cgaagcttaa
    15721 acagctcatc aatgtttttt agcccagaag ctacatttat tttttgctct acaaataagg
    15781 tttatggtga taatccaaat ttcttgccat tcgttgagca agaactgcgc tgggaaatag
    15841 aggagacgca tccttattgg aacggcattg atgagtccat gagcatagat aattgtaagc
    15901 actctttatt tggagcatct aaggttgcag ccgatatact tgtccaggaa tatggtcact
    15961 attttggtat gaaaactgct acatttcgag ggggatgttt aacaggtcct ggccactcca
    16021 gcactgaatt gcacggcttt ctttcttact taatgaaatg cacgataaca ggagctcatt
    16081 ccacaattta cggttataag ggtaagcagg ttcgcgataa tattcatagt tacgatttag
    16141 taaacgcctt ctatcaatgc cctgtttttt acgatgttaa ttatgctccc attcaacact
    16201 aaatggggat taggttgggg ggggggaaat tactatggga aaggtataga cgcagttgaa
    16261 aaggaccttc tggctggcac tccccttact gccctcgcag aacgccaccg agactttctc
    16321 attcatagct tgaataaaga tgagcttgca aaactaatgc agatgcttca cgatgctggt
    16381 attggtccct tcgcacagat gattggtgac cagtccaaac aggggtatta acagtcctgt
    16441 tatttgcgca aatattccaa ggcatcctta atgagagaat aagatcgata ctcagtatgg
    16501 tttcaaggct ttaacagcac aagctgcaaa cagaaccaat cactagtatt cgagaaatat
    16561 gttgatctat actcactcag actagcaaca aagctgtcaa attaaacttt atgttgtatt
    16621 tcaagaatga taatccctga attttcgccg tctagacgcc taatttacat atttgatttg
    16681 cttttcgaac tagtcgctcg cgatatgaag ctgcgttata agcgctcggt gctaggcatt
    16741 gcttggtcgc tgctcaatcc actttttcag ttacttgtct tactcttcat attcaacatg
    16801 gtcgttccag ttaatatccc aaactacttc tcattcctct tcaccggcct tctctcctgg
    16861 atttggttcc aatcctcatt gctcctagct acaggcgcca ttgttgaaaa ccgcgatctc
    16921 ataaaaaaac cgggcttgcc tgctgccatt ctgcccacag tcaccgtcac aagtagtctg
    16981 atccatttcc tgatagcgat tccaattcta ttgatcttcc taaaacttga cggcattcga
    17041 ttaacaagtg catctctagc cttgcctata gtaattgcga ttcaattcat cctaacgctg
    17101 agccttgcat ttcttctagc cacactccag gttttctttc gtgacaccct atacctgcta
    17161 ggtgtttttc tcaatcttct ctttttttta atacctatat tctacgacgc aagcaaggtt
    17221 ccagaacgtt tcaaaacact ctacaatctc aacccgatgg ttcacataat agaagcttac
    17281 cgtactatct tgatccgtgg ggagttgcca gataacctat ccttgctatt agtcggtctg
    17341 cttgctactg gattattaat cttcagctat aagattttta aaagggcgag ttactacttc
    17401 gctgaggaat tatagatgag ttatgcgata gtagttaatg gcttgagcaa gaaattccgt
    17461 cgctaccatc ccgatcggcc ccttacgctt caagaggcta tcctaggcgg tttaggtcgt
    17521 ataaagcctg tggaatacct ttgggctctt cgcgacatca gttttaaaat tgagtcgggt
    17581 cgtacggttg gcgtattcgg accaaatgga gcagggaaat ctacactcct tcgactcatc
    17641 agcggcgttg gaaagcctga tgcagggagt gtggtagttc gagggcggat tggcgcgcta
    17701 ctggatttgg gtgctggttt tcacccagat cttacaggaa gagagaatgt attcgtcgtc
    17761 ggggtcatct ccggtctcac gcgaagtgaa gtttcagaga gatttgattc aattgtggcc
    17821 ttcgctgaac ttgaggagtt cattgacagc cctgtgcgaa cctacagcac gggtatgctt
    17881 atgaggcttg ccttttccgt cgccgttcat gtggaaccgg aaatattgtt aatcgatgag
    17941 gtattagccg ttggcgatat tatgtttcag cgcaagtgtc ttgagcaaat cacaaaattc
    18001 aaagctaagg gctgtactat catcctagtc actcatgaca cggcgcaaat tagaaatctc
    18061 tgtgacgaag caatatggct gcacagggga cgagtagtag cacgcggacc aacgaatgcg
    18121 gtagttgacc agtacatcgc tgaggggctt gcagaaacga aacggcgcac accagcagaa
    18181 tggcctgttg cacatacaga cgcaggcact gagcttcgta ttaatgaaaa ccgttttggg
    18241 tctcttgagc ttgaaattac agccgtgaaa ctcttaaacg gaaaaggttt acctacaaat
    18301 gaactaaaaa gcggagacca attgtgtgtt gtgattgcct acaacgcatc tcaaccgata
    18361 cctggtccaa tcttttttat caacattaac cgtgaggacg ggttattatg ttatgatact
    18421 accacttcag gagctgggct aaatctacct acgattcagg gcaatggtca ggttaaacta
    18481 tatattgaac gtcttgatct aacacgcgga ctatactatg taaatgttgg agtatgcgag
    18541 cgagattggg cttactctta cgattaccac cagcgggtat acccattagt tattagttct
    18601 gacggtggcg aaaagggcat aattcaccct cctcacaggt gggagataga aggggagcaa
    18661 aaactccaaa ccactgtacc aattcgtaag gctccttgat tcaataggtg caggttttcc
    18721 cctcacaagg ttttttagaa cttatataat gcctcaaatc caatatcttt ctatctaaaa
    18781 gatcttactg ttatcctttg taaatctctc caaataaaaa tgtattcaaa ttactaatca
    18841 acaaccctta gctgcggggt atccgccgtg taagcaggcg gtccattcgg tcatcgcgag
    18901 gaatgagttt acaacgaaaa ctctgttcag tctttgcaat gacaaccccc tttgccgtcg
    18961 ttcgagggat tgcttcgcta gcgctcgcaa tgacattata gcatgaaccc tgtagcaatg
    19021 aggctgtccg acaattatgg atcgtaaaaa tttgacccct cagaatgccc caggatcaac
    19081 gatcggcaac ctgggagggg ttttgctacc cccaagaatg ccgatttttg catttatcgg
    19141 acaacctcca atcctccggg ttaaactggg ggacaggaaa ttatcaggtt aacgattaga
    19201 acttgcgctt cacttcaaaa aatggagcaa aaaggtaaaa ccgtccagat agaaagggtg
    19261 cttaagtggt gggattaccc tttatttatt ctgttaactg ccattttaat ttttgcgatc
    19321 tataccttta tatcttattg gctatccctt aaagactggc tttactaccc ggcaaccttt
    19381 gtaatgatca ctctaatgct gttaataatc ctcggtaaca gcctgcttcg ttggttgtta
    19441 ttactttaca taagaagacc aaaacctatg atacccagtc ctagtttgaa agtgggcgtt
    19501 gctaccacat ttgtaccgag cgcagagcca cttgatatgc ttgaagatac acttagagcc
    19561 ttgattgcct tggactatcc acatgataca tgggtattgg atgaaggaga taacgacaag
    19621 gtcaaggtat tatgccaacg actcggcgcc tatcatttta gtaggaaaaa tctaaaacat
    19681 tatcaaatgg aaaccggacc tttccaatca cactcaaagc atggaaatta taatgcatgg
    19741 ttatacgaaa ctgggtttaa acaatatgag atcatatcga tatttgaccc ggaccaagtt
    19801 ccagaggctg cttttctttc aaatgtgctt ggttatttcg aggatccaaa ggtaagttat
    19861 gtacaggtag cccaagctta ctacaatcaa aaggcaagct ttattgctcg gggagcggct
    19921 gaagagtctt acgggtttta ttcttcaatt caaatggcaa gctatggcat cggacaacct
    19981 agtatcatag gatgccacag cacgcaccgc gtggctgctt tgaaggaagt aggtggtctt
    20041 gcaccccacg atgcagacga tattttactc acgctacttt atcgaggaca tggttggaaa
    20101 ggagtttatg tcccgaaaat tctcgctaga ggcttggcac ctgtagattg gaatggatac
    20161 cttactcaac aactccgttg ggctcgttct ggtttagata tagcgtttcg tattcattca
    20221 cagttatcaa tgaacttacc cctaacgaag cgtattatta gcgttttaca tggtcttaat
    20281 tacttatata aaagttttct catttttacc ggtcttgtaa ttttagtttt catgcttgcg
    20341 acagggatca caccaaaagt ggtatctttc tccactctac caaggcttgc tattttatgc
    20401 ttggctatac aaatttgtgg attctataga cagcgattct atctagattg gcgtgcggaa
    20461 ttgggaatcc actggagagc ggtattgtta cactatgcaa aatggccgta tatgttttca
    20521 gccttctttg atgtgatatt gaaccgccga gtgccatatg cattaacctc aaaagtgaaa
    20581 gaaagaccca gatatcagac cttgctttgg ccacatatcc ttgttgtcat tttcattgga
    20641 atttctatgt ttgttggttt ggtttcagga cataacataa acccattcct tcttatatgt
    20701 gctgccataa ttgttataat ctcgcttgcc ttaattttaa cggagcttct gaattttccc
    20761 gacccatatg atagaaaact acgaaattat ttgaatataa agcatgtgta aacatggagt
    20821 ttgataaaga gtttcaagaa aactcgaaga agtcctcccg gacgacggca ggatactatg
    20881 cgttagcgtt actgaccaga ttttgagtga caatctagcg caggatggca atatttgtcc
    20941 tcaagcgtta aggccttaaa ggacattctg ctctcggcat acaaattgtc ccgatggtta
    21001 caagcagaag gatattgtgg agtagttggc tttgatttcg gagagtactt taatccagga
    21061 acaggagagt tcaagcactt cctcgccgaa gttaatccaa gaattagctc accgacttat
    21121 cccaagtccc tgatggaaca tctcaataga gaacagggac ataaaggctg accatgtata
    21181 gagttttttt attcaacaaa tataaaaaca aaggcaagct catttaatga accaagtaaa
    21241 ttgtacggca tttattcttc aagcctgaaa caggaaaagg attagttccc ttcaatattg
    21301 gttgtctgga gcgtggaaaa tttaccctaa cagtttttgg taaatccaaa aatgaggtta
    21361 cggaaatata agagtatttt aaggtattgt tagccaaaga agaggagatt tagctccgta
    21421 aaaacgaaaa gccagtgatt tggaaaacgt agcataagaa gcaaatcaat ggggaatgtc
    21481 aaattatctt tttttgctga gcttaccgca cataactgct agacattaga ctgtatttta
    21541 aggtgatata aacagtatat gcaaaaaatg gaataacaat atatgtcaga gcaattctcc
    21601 aaattcaaag aaaaatttga tcctgaggaa tggagtgata tttataaatc ctcaaaaaga
    21661 aataataaag cttttatatt caagcatggt agtgaacttg tagaagatac ctgctctaaa
    21721 ataagtcgac caaatgagtt gtggctcgat atagggtgtg gtacaggaga tttagcagta
    21781 aaattatctg agatgggatt atctgtaatc ggggtagatc atgatacaag aatgatagag
    21841 tttgctaata agcgtttcct ttggcatagc atagtgaata gactaacatt tacaaccgcc
    21901 aaggcagaat ttcttccctt ttttgataac actacagatg gtattgtagc tgtttcatta
    21961 gcaggttgtt tatcatcacc agataagttt tttcaagagg cctatcgggt tctacgtaaa
    22021 aatggctttg caattattac cttcacaaat cgagccagtt tactacttaa aataaattgg
    22081 tgtttgaata agacattgca catgattaga aaaccaactg atagcgatga tcaatatcaa
    22141 atctatgctt gtactgaagt agcggataag ctacagaaga tcggatttag aattcttaat
    22201 actagatttt acaacttttt tctcaatgcg ggggatagga tgatcccacc tgagtggcta
    22261 gcaatacatt gtgaacattt caataaatac aaaatgggtc atcgagtggg cggaaatttc
    22321 ataatagtag cgcaaaaaat ctagggcaaa atagcgagaa ggaggattta atagtgaata
    22381 aggtttcttt ttctctacta aggggcgtct gtcagatgcc agcatatgtt gcatacgaaa
    22441 agggtttttt caaagaagaa ggtcttaatg ttaatttaaa tatcgaacct acagcatggt
    22501 tggttcctca taaacttgta agtggtgaaa gccactttgc agtaattcca tggacaagag
    22561 ttgctgccgc agaagataag gatacacctc ttgtattgat ttgtggttca ggttttgaag
    22621 aagctgtcat agttgttcga aaggaaataa gcacttcaaa ggtgagaaaa gttgccgttc
    22681 cattacgtgg aggcatgaaa gacctaactg caatggcact cattgaaggt ctaggatgga
    22741 aagacgttga gcttattagg cttccctctg gtgatggagc aattatttcc ctttttggta
    22801 gaggagcaga cgcggcttca atggtggaac cttatgcaac aatgatggaa gcattaggca
    22861 tcggaaccgt cgtaaaaaga acgggggatg tatggaaagg ggctcccgga tgctctttaa
    22921 caacaactat ggagcttaaa gaaacttctc ccgatctgac acaaaaggtc gttagagcat
    22981 ttgtacgcgg ggttacattt gttaaagaaa acccagacga gtctgcccag atagcttccc
    23041 gctacatctg cattaatgct ggatttatcc gtaaggccct gaatgtaaac aagccagatg
    23101 tggaagccat ccggaataag aaggctatgg aagaaatcct ctctcttatg atgaagctgg
    23161 gttacataaa aagacttcca gaaggttatg ttgacttaac atttcttgat aaagcaatga
    23221 aaaggatatg aagagaatgg acaagcggat aaggcagagt ttttttgata gctgggaagc
    23281 tggtgaggaa tggaagggag tttatttcaa agcttcatga agattgggct tacaagcaga
    23341 agtataatga ggcggaggga aggcttaagg gtttggcttg ggttagatag cagtgagaga
    23401 agtaagatag aaggcaagag ggatacaaag agataaagac cttctatggc aaggtttgct
    23461 taattgatag aaaaggcata ggaattaagg tctttgagct atggatgaag gcaaaaaata
    23521 gtttcttttt aaggaagcag gcaattggag agaaaattct tcttaaaaga gattattctt
    23581 tgtgagacaa taagaataga aatggtttat ggataaaatg gaataatcaa acacctttta
    23641 taagggtcta ttattatgga tcaaccgctc cgcttttgta tgatcactat attctatcca
    23701 cccaactact ttggtggcga tggtttgttt gtgttcatgt aaaaagaaga atccaagagg
    23761 ttttaaatgc tgtatttaag gctttggatt tttagggtaa taactgagtg ccagaatatt
    23821 ttactggaca gtttcttatt ttttcatggg agatatttgg agtgaaattg ttccaggtat
    23881 ttaaacctaa ataaatagaa aagtgttgat ttatgcaatg aaatgtggtt actctggtta
    23941 atacaaagat gagtttgaga tagttttaag gggtaaaatt acaaaacaac aaatgcctga
    24001 cacaaggttg aaccatcttt atcttcataa gtgtcaatca ggacaaccaa tttgaatgaa
    24061 gaattgaaat tactgaaacg ggcactgaat aatgatctga tattgttttt ttcagttttt
    24121 gttattggcg ttcttcttcg cttcaggcat ctcacttcgg gattagtaga atctcatagt
    24181 cagcgccagg cagatcttgc catgatcgct tcaaactttt atagtaacgg ttttgacatc
    24241 tttcaccctc aaattgattg ggccggatcc tttcctggat atattggaat ggagtttcca
    24301 cttgtacctt acctgatggc tttgctatat gctttgtttg gcaagcacct atttcttggc
    24361 cgccttctct ctatcagctt ttgtgctcta tcaacctatt atttttattc attagtcaga
    24421 agttacttca cgaaacgaat tgctttaatt tctttggttt tcttcctctt ctctccggtt
    24481 agtatcttct tctctagaac tcttatttcg gataatgcga tgttatgttt ctcgatcgga
    24541 gccatatact ttttttctca atggacgaaa aatgaaaaaa cactgtattt cattctgtct
    24601 ctcattttcg ctatgcttgc actacttgtg aaaatacctg ctgcaatcat tggactccca
    24661 attgcatatt tggcctggta taagtacgga tggtcgtttc tgaggatgac tagactttgg
    24721 atatttggat ttattgtgac aattccatct attctttggt atgtgcatgc atttacattg
    24781 gccaaaaata attacccatt catcatgttc ggagaaggaa ggtgggtctg gtatagcgga
    24841 ttcgaaattt ttctagacgt agcatttttg aagaaagtct ttgtaagatt gacggctttt
    24901 ttgatcactc ctatcggcct aataatgtcc ctcgcaggtg caattacgac tgcctggaat
    24961 aaacctaaca cttatctctt tcactggtgg gcggcaggtt tttttgtttt ctttattatc
    25021 ggatttccag gacaagggca cgagtattat cagttgcagc ttttgcctgt agcatccccg
    25081 ttcgctgctt tgttctgcga tgagctcatg aaatatagag gtgcatacaa ttcagggatt
    25141 ctgaaacctg ggttacgaac tgtgatagta atctgcttga tcgtaggatt cctggtttca
    25201 tctttagcaa tagttgagac gcgataccat tcttctatcg agaaaatcct ttacgaaggt
    25261 ggattgtcag tacaatccat agtaccagtg aatgctctca tattggcagc tgattgggat
    25321 aatcctgtac ttatacatgc aagtaaacga cgagggtggc acttcccggg ttccaagacc
    25381 tataaggatc tagtacgaac ctggagtgtc tctctgttag aaaaaatgcg atcacagggc
    25441 gcttcttatt ttgtggcacc catcaaattg ggtatgaatc ggcagattgc atccgagaac
    25501 tttcttcagt cggttggacg acgaattctg cctcctagag atgtggattt ccttgaatcc
    25561 caccccgcat ttaaaagata ccttgatagc cattacaaaa aagtattgag taatagattc
    25621 gtcgtagttt acgatatgag gacaaaggca aatatgtgat cttttaaaaa aagaggattg
    25681 ttaacctctc aaatggattt tattcaagat ttgtttacac gatatgacaa atagtatggg
    25741 ttaaactaag ctctctaaga aatagagggt tttctgctgc ttgattacaa ttctcattaa
    25801 aatgggcaga taatactttg aaaattttac ttgacaggta ctgttagagg ctgctaaatc
    25861 taaatgtgta aagcttctac ttggtggaaa tcaacgaaca gataatgtat tcccaaagcg
    25921 aaacagagcg gttacggaaa agaatagaga aggcctggat tacaggagtt ccttgagacg
    25981 aaattgatac caataggact ttagacctga taataattaa aggaggtaaa taatatgctt
    26041 agcaaaatgc tcgacacagc aatgaaaccc atagccaggc ttttgcaata caattatgat
    26101 gcgaaaccta tgaagccatt aggcgtcaat aatacaatga aaattgcgcg agcttacaat
    26161 cgctttaagt atggtaggta ccatgaatat agaaaaagcc agtatctcca tcatttcgat
    26221 aaacttatcc gcgagaacgg attgcctttt tctccactta acgaaataaa agacggctgg
    26281 gcgctagaca cctctaaaac actgcctcac ctcaaacagc tattagccga tgcagaagag
    26341 attattaagg agcgagcggg tgtaaaacgg acggactttg gtagaccata ctttcaagaa
    26401 attccgtttg acgatttatt ggtcaaatac ccgtccattc ttaactttgc gacctcctcc
    26461 gatgtgcttt caactatctg caattacctc ggcttaatcc ccgtcttctc tgcggctcta
    26521 ccacctggga ttaggtttaa tgaaacatgg gcaaagtttg aggatgtgcc agacggtccg
    26581 cctaaatcaa gtcagctatt tcatcttgat tatcatgatt cccctatggc ctatgcgctt
    26641 gttctcctta gggatgcaac aatggaaaac ggaccattct gcatactgcc cgaatctgta
    26701 tcaaaaaggg cctctgaagc cttgaactat agagctaggg gaaggcgtca tcgcgtcact
    26761 gatgatgaga tgtattcggt agttcctgaa agcgagctaa taaagatgtg ctatcctgca
    26821 ggtaccgtta tgttcctgga taacagcaga tgtttccatt acgggagccg aaatgcagtg
    26881 aaacctcgtt atcttttgat gttggcttat atatctccat gcagaacgga ttttggtgag
    26941 gtgctcctag aacggaaaat atatccagtt cgagataacg actctcgttt gagaaagatg
    27001 gttctaaata gagaattcat ggaatgaggg gctattatgc taaatgattt aacaatgttt
    27061 gcaagatatg cccgggggct taatagttat ctgcgccaca cgcttagccc cgagcagtgc
    27121 cgccagatgc ttgtaaatca tctcgaaagc agggatgaat cctttttaca tatactagaa
    27181 cgcggcattt atacaaatcc acgaagtcct tacagaaagc tactccttca tgcaggggtt
    27241 gaattcgcag atgttgtcag cctggtgaga cgacacggtg ttgaaggtgc gctggcacgg
    27301 ctttacgatg cgggagtata cgttactctc aacgagttca aaggacgtcg accaatcgag
    27361 cgcccgggac tccaaatggg agtccgtcct cgggattttg ataatcccct tttggtcaag
    27421 tactacgagg cccggactgg cggttcgcga ggcgttggaa cccgcatcat tattgattta
    27481 gacctcctga ctcatgaggc agcctactta cactactttc tggcggcctt tgacctggag
    27541 gaaacttcga tcggggcgtg gcatgaggtt ccgccggtag ccgcaggtat gaagctagtt
    27601 cttcgttacg tcaagcttgg aaagtctgta gaaaggtggt ttgctcaaag taggttaggg
    27661 ttaaggccag gggacttgaa attctttatt tttacaaaat acaccatcta cgccagcgga
    27721 attctaggca aacagttacc ggtaccagaa tatgtgcctc tagaagaagc ctccaaggtg
    27781 gcacattggc ttgccataaa gaaagggaat ggaaaacctg ccactctgga tactaatcca
    27841 agttcaggtg taagagtgtg tctcgctgct aaagaggaag ggctcgacat cgcgggaagt
    27901 ttattccgcg tgggaggcga accatacacc ccggcaaaag cccaagtagt agccgaagcg
    27961 gggattcgag ccgtttgcca ttactccatg tcggaaattg gcactattgg caccgcctgc
    28021 acttcccctg ccgatctcga tgatgtccat ttgctcaccg acaaggtcgc ggttatccag
    28081 cgtccaaagt ccgtgggtac cggtgagcta agcgtgggag cccttgttta tacgacccta
    28141 ctgcctagtt gccctaaact aatgctcaac atggaaagcg acgattacgg cgtcctggag
    28201 aacagggttt gtagttgccc tatcggcgag cttggattca ctaaacatct ttcgcgtatc
    28261 cgcagttacg aaaagctcac gagcgacggt gttacatttc tcgggactga acttctacgg
    28321 ctggttgaag aggttttgcc tacgagattt ggaggttatc ccacagacta ccagttggtc
    28381 gaagaggagg aagaaggact accaaaagtg aacatccttg ttagcccgcg tgtgggtgat
    28441 gttaatgaag aagacttgct gtctacggcc tttcaggttt tacactccta tcctggtgga
    28501 gacatcatgt cggaaaaatg gcgacaggga cagaccctgc gtgtagcccg cagagagcct
    28561 tataccacgg cttcagccaa aattctgccc ctgcacatac ttcagaaaaa atgacctccc
    28621 gtgagggaaa tagttctcga caatgaaaat cctttttttg atacaaagtc tagaaatagg
    28681 tggtgtaggc cgtcaactct caattctaac caatcattta gcccttcgtg ggcatgacat
    28741 gtccgttata gccttgtacg ccacagacca aaattggaaa ctgatttgga aattagattc
    28801 tattgatata cggtcgcttc ttttaaataa acctacaggt gttttagccg caggtataga
    28861 attaattaag gcaaccctag aactaaggag tttgctaaaa agggaaaaca tccaaatttt
    28921 gtatggttat ctgggtcaca ccgccagatt tatcgcctgg cttgcaactc gcggtatgcc
    28981 taataccaaa ttgatatggg ggacgcgaac atccggagac aatacggatt tgtaccctag
    29041 aaattggaag gtatctttcc tattcaattt gtgcaaatgg ggttctgcat cagttcctct
    29101 aatgatatca aattcagagg cgggctatgc ctatcgcaaa gccaaaggct acggctgtca
    29161 gaaacatttg gtcataacta atggattcga tgtagaaaaa tttaaaccgg atccccaagc
    29221 acgtgtacga atgcgttctg aattgggcgc tcttgagaac gagaaattaa tcggacgagt
    29281 tgggcgactg gacccgatga aggggtatcc tgatttcctt gaggcagcag cgttattagc
    29341 caaagaaaga aaagacgtac gttttgttat tgctggcgat gggcctgata cctataggag
    29401 gcagcttcag cttttaagcc aagaattagg attgacggaa cgtctgatat gggctggggt
    29461 aagagaagat atgcctgctg tctacaacgc gctggatatt gcctgctcct cttcttctta
    29521 cggtgaagga ttcccaaaca caattggaga agcgatggcc tgtggtatcc catgcgtcgt
    29581 cacagatgta ggggattcag cgaagatagt aggtgatgag ggcataatag tcccatccgg
    29641 ggacccaaaa aagctagcta atggattaaa aactatgctg ctgcaacttg acgatataaa
    29701 accccatctg cttcgtgagc ggattatgaa ccgcttcagc atagaaacca tggtcgaatc
    29761 tactgagaaa gctttgatcg aagtatgtaa tacttctaag taaggaaaaa atgatctctt
    29821 gtgaggaaaa tagttctcgg caatgaaaat actttttttt atccaaagcc taggaatggg
    29881 tggggcagtc cgtcaactct ccatactgtc agatcattta gtccgtcgag gtcatgatgt
    29941 ctctattgta gctttgtata ccacagacca aaattggaac ttagtctgga aaattgattc
    30001 tattcaagtg aaatcttttc ttctacagcc atcgacaggc gctttgtata ccggtttaca
    30061 attaatcaag accgcagttc aactcaggaa catgataaaa agggaagaaa tccaagtgct
    30121 ctacagttat cagggggata ttgccagatt cattgcctgg ttggctaccc gtgggatgcc
    30181 caataccaaa ttaatatggg gaatccaggg gtctggtcaa agaaatacat tatccggtaa
    30241 caactggaag gtgtccttgc cgttctattt gtgcaaattg gtttcagcat ctgtcccgct
    30301 aatgatctca ggctcgcagg cgggatataa ctctcgcaaa gccaaaggct actcttgcca
    30361 gaaacaaatc gtcatattat atggaataga tatagatcag tttcaaccag atccccaagc
    30421 acgtgtacaa gtgcgttcgg aatggggtgc tcttgagaac gagaaactaa tcggacgaat
    30481 tgggcggttg gccccagata aggggtaccc taatttcctt gaggcggcgg ctttattagc
    30541 taaagaacga aaagatgtac gttttgttat cgttggcgat aggtctgata gctatagaag
    30601 acagcttcag cttttaagcc aagaattagg catgacggaa cgtctgatat gggctggggc
    30661 tagagaggat atgcctgctg tttacaacgc gctagatatt gtctgctcct cttcatacag
    30721 tgaaggcttc ggtttgacgg ttgcagaagc tatgggttgc ggtgtcccat gcgtggtcac
    30781 tgatgtaggg gattctgcga ggatcgtagg tgaagagggc atagtggttc cacccggcga
    30841 ccccctaaag ctagctgacg gattaaaaac tatgttgctg caacttgacg atataaaacc
    30901 ccatctgctt cgtgagcgga taatgaaccg cttcagcata gaaaccatgg ttgaatctac
    30961 tgagaaagcc ttgattgagg tatgtagtgc ttttaagtaa ggaaggggat ctgacaataa
    31021 tggcttcttc aatacctatc ttaacattcc atgccgttga tggccgatcg tcggtcatct
    31081 cactctcacc gcgggtgttc aggcgcggta tggccacact gcatgaaaac ggatatcaaa
    31141 cgctgagttt gccggaagca gtaaatttca tgcgtcaagg aaagccattc ccagaccgct
    31201 ccttcgtgat caccttcgat gacgggtatc aaaccgtgta tgacgaggct atcccgatat
    31261 taaagggata tggcatgtcg gcaacagtat ttctcactgt aggagaaaag agtgcgtcaa
    31321 atcctgccga ccggttgcct tctctcaacg gacgctctat gctgaactgg cgagagatcc
    31381 gagagatgaa gcaatgggga ttcgactttg gctctcacac gctcacacat ccagacttga
    31441 ctcgtttgcc attagaacga gttgagatcg aaatccataa ctctaaggcg ataatagagg
    31501 atgcgcttag cgacctcatc aattgttttg cctaccccta cggtcgctat gacaatcgaa
    31561 gtcgagaaat cgttaagaag cactttgcct gtgcctgttc cgacacgctc ggcctggtca
    31621 atcaaggtag tgatctctat gccctcaaac gaattgacgc ttattatgtc cgtaccgatg
    31681 ggttgtttaa tttgatgtta actaaatttt ttccctggta tatcaaagca agaaacatcc
    31741 cacgtagaat ccggcgcgcc attctactcg gcttgagtta atgaacacac ctctccgctt
    31801 ttgtatgatt acaacattct atccacccta caactttggt ggtgatggca tatttgttcg
    31861 ccaattgtct aacgagcttg ccaagcgagg tcatcatgta gatgttatac actgcattga
    31921 ttcgtaccgc cttttggcac gtcaggagcc aacgaagacc tatgatgatc atccaaatgt
    31981 aactgtacat ggactgaaga gtcggctcgg ctttctctct ccgctgacta ctcaacagac
    32041 aggctaccca ttctttaagt cagcctctat tcgacggatc ctagaaaagg gctttgatgt
    32101 gatccactat cacaacatct cgcttgtagg cggaccaaaa atcctggaat atggacaggg
    32161 tatcaagctc tacacaatgc acgagtattg gctcatctgc cccactcacg tgctatacag
    32221 gtttaaccgc gcgccctgca cacggccgca ttgcttcctc tgtagcctta cgtataaacg
    32281 cccgcctcag tggtggcggt atttcggcct gttggaaacc acagttaaaa acgtggatgc
    32341 ctttattacc ctgaaccatt tcagcaaaga cctgcattat cagatgggac tcaacgttcc
    32401 gatcgtgaat ctgccccctt ttgttcctac agcagagggt gtgttgtcaa catccgaaaa
    32461 acaatttgag gaaacattgc tggagccgta ttttctattc gtgggacgac tagagaaact
    32521 aaaaggcctg cagacgctca ttcccatctt ccgccactac cgcaaggcgc agttgttgat
    32581 tgccggcaca gggaactatg aacccgaatt gcggcaattg gcgaatgaca gtactaacat
    32641 caggtttctg ggttttcttc ctcaacagca actacagctt ctgtaccaac aggctgcggc
    32701 tgtgattgtt ccatcattat gcttcgagat tatccctctg gtgattatcg aggctcttag
    32761 agaaaaaaca ccggtcatag ccaagaacct gggcgggatg ccggagatta tcgaagagag
    32821 cggcggcgga ttcgtctacg acacggacga ggagttagtg gcagcgatgg accgcctatt
    32881 agcggaacca tcatatcgac gcgaactggg tttgcgcggc tatcaggtct atcaacagaa
    32941 atggacccca gaagctcacc tgaagctgta ttttgccctg atacatgaaa tctcttcagc
    33001 taaaaggctg aggatcagtc agcctaatgc ctagccttaa ggcagcacta attgggacag
    33061 gacttcagta tagtgtgaat aataagtggc tgctctaact agcaacctat accaaatcgg
    33121 ttaattactt tagcaatcag ggtttcatca tagtcatgtt aatgagagca atgcggggca
    33181 aatgatatga aacacataga acctctcata tctgttgtat ttgggaattt ggctgattcc
    33241 tcgaggattg cggcgccgga ttgaattcgt tagaaggcgc cgtaacagtg tagtgaagct
    33301 gggcttaccc aggaagtaaa tagccaattc aagccttttt aagacgacat caatctaagg
    33361 acatgagaaa aaactcacgc tccgaagtag aagaaaaggt caaacatatt ctaatttctc
    33421 aactcgagat taggcccggt atacttgctg agagtagttc aactacgcct cttttaggac
    33481 gaggtattgg tttagactct atggaagtta tggcactcgt tgcggcactt gagacagaat
    33541 ttgatattga aattcccgac gaggatttaa ccacagaact tgtcaaagat ataggcaact
    33601 tggctgagta cctcttgcaa aagatttcta agggataaaa gcgccaactt atgaagatgc
    33661 acaccctgat gattccatgt tcaccatctt caaccacgaa atttatattt gctgacttac
    33721 aaacatatct attgataaat catcccttca gagggccgaa aataaaatat gagaaggagc
    33781 aatgggtcta gcatgcttcc tagggctgtt ttattggttc cagaacctga atcaacacca
    33841 acggctcttg gtataatacg atccctgggg cgtgtcagca ttccagtagt agcggcagcg
    33901 tcaaacctga caacgccctc ctttttctcg cgttatgtta aacaccgtct tgtctgccct
    33961 gccctggagt ttgagcccat caattggaaa gatgcgttga tcgaatttgg aaagtctgtg
    34021 gatgagaaac cggtaaggtg ggtcagtttg ttcgacggaa aaccacgagt tatcgtaagt
    34081 tctttcatca atttggacaa aaggcagttt ccatacgaac actggaggaa ctcggaaaca
    34141 taattctacg tgggactagt aggaata
//
LOCUS       DATDRL010000123         3879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_311395, whole genome shotgun sequence.
ACCESSION   DATDRL010000123 DATDRL010000000
VERSION     DATDRL010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311395"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..339)
                     /locus_tag="VI935_04390"
     CDS             complement(58..339)
                     /locus_tag="VI935_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24872.1"
                     /translation="MTDFDIAEKLEELFRKTGSEHHKAFVKTNGEDPEWPLWYAEYLF
                     EKLGKLLNAKFTKSELVYLLVMVEKERALMAPGADWPRYYTNFFIERYI"
     gene            complement(356..1126)
                     /locus_tag="VI935_04395"
     CDS             complement(356..1126)
                     /locus_tag="VI935_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLE24873.1"
                     /translation="MNLEDFIILIVAGLFSGFVNTLASSGSAITLPLLLFLGLPANVA
                     NGTNRIPILASSIISVLTFQHAKVLDWKNGIRLGIPVVIGSITGSFLASIISSKTLNW
                     AIIAAVVFAFFILLLRPKRWLKEKPAEHLNLVWIQMLILFVVGLWAGFIVLDSATYML
                     FALVLGVGYDLIRANAIKSLFLFFVSFSSLLIFYEEHEVNWKIGGILAAGSMVGGWFG
                     AKMASKEWAKTWIYRALLIVILGELIHLLYRFVFIHKL"
     gene            complement(1175..1312)
                     /locus_tag="VI935_04400"
     CDS             complement(1175..1312)
                     /locus_tag="VI935_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24874.1"
                     /translation="MSDIDIAEKLKDLFRNTGSTHNQAFFKLNNEDAEWLLWYYADYL
                     P"
     gene            1566..1775
                     /locus_tag="VI935_04405"
     CDS             1566..1775
                     /locus_tag="VI935_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HLE24875.1"
                     /translation="MVRVNITLDEELYKELSSIKNKSEFIRAAVREKLRIIKEENLKK
                     VLKEGYKGEGKNLKEWESTVADAWD"
     gene            1762..2115
                     /locus_tag="VI935_04410"
     CDS             1762..2115
                     /locus_tag="VI935_04410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HLE24876.1"
                     /translation="MRGIKRGSVWLVSVEPIIGRGIGKTRPAVVISNDINNEYAETVT
                     VIPITSSVSKVYPFEVFLSKGTANLPKDSEAKCNQIRSVDKRRLIRQIGRVSQQTIKE
                     IEKALLIHLGFEMYT"
     gene            complement(2374..3111)
                     /locus_tag="VI935_04415"
     CDS             complement(2374..3111)
                     /locus_tag="VI935_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24877.1"
                     /translation="MKCKLCLKEKPLIKKSHIIPDFMYKELYDERHFIYQITLGGKSK
                     PKRKPTGEYESDILCGECDNKIINQRYENYASKVYYGNEETTGIKHRRERSENSVLEW
                     TVVKNISYEKFKLFLLSILWKSSISNSVFFKEVKLGLHGEEIRKMIINSDPKNHHDYP
                     CILLHAKHQELDVKQLIIPPKRFKDGKTTIYKFFISGIFYIYYIPTGKEPEFLFEGAI
                     NKSNTMKIFHFPEDQHNYSKLNKVLFY"
     gene            complement(3453..>3879)
                     /locus_tag="VI935_04420"
     CDS             complement(3453..>3879)
                     /locus_tag="VI935_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLE24878.1"
                     /translation="PLLGIGSAPAIVALFLYSLLPIVRNTYTGLQDIPVGIRESAEAL
                     GLPSGARLRLVELPMASRSILAGIKTSAVINVGTATLGALIGAGGYGQPILTGIRLDD
                     VGLILEGAIPAAVLALLVQGLFDLLELVVVPKGLRLKSD"
BASE COUNT         1231 a          679 c          722 g         1247 t
ORIGIN      
        1 catttgattc atgagtgagg ttagaaaaat ttattacctt ttattaatct taaaaaccta
       61 tatataccgt tcaataaaga aattggtata ataccttggc cagtcagctc caggtgccat
      121 gagcgccctt tctttttcaa ccattactag taaatagact agctcactct tggtaaactt
      181 agcattgagt agctttccaa gcttctcaaa taaatactct gcataccaaa gaggccactc
      241 aggatcttct ccatttgtct taacgaaagc cttgtgatgc tcacttccag tttttcgaaa
      301 aagctcttcc agcttttctg cgatgtcgaa gtcagtcata acgttttctc cttatttata
      361 gtttgtgtat aaagacgaac ctgtaaagta gatgaatcaa ttcccctaga attacaatga
      421 gtagagcacg gtaaatccag gtcttagccc attccttgct tgccattttc gccccaaacc
      481 agccaccaac catgcttcca gcagcaagaa taccaccgat tttccaattt acctcatgtt
      541 cttcatagaa gataaggagc gatgaaaagc tgacgaagaa gaggaaaagg gattttatgg
      601 cattagcacg aataaggtca taaccaacac ctagcactag agcaaatagc atgtatgttg
      661 cagagtcgag gactatgaag cctgcccaca agccgacgac aaacagtata agcatctgaa
      721 tccatactag attcagatgc tctgcaggtt tttctttcaa ccagcgtttc gggcgtaata
      781 atagtataaa gaacgcgaaa acaacagcgg cgattatagc ccagttgagt gtcttcgagg
      841 aaataattga agctagaaaa gatccagtaa tagagccgat tacaaccggt atgccaagtc
      901 ttatgccgtt tttccagtca agtaccttgg cgtgttggaa tgtcaaaaca ctaataattg
      961 atgaggctag aattggaatt cggttagtac cgtttgctac attagcaggt aatccgagga
     1021 agagaagaag aggcaatgtg atagcagagc cgctgctggc aagtgtatta acgaaaccag
     1081 aaaagagacc agctacgata agaattataa aatcttcgag attcattaag cgttcatatt
     1141 tgactaacga atttctgacc tactttctat gcttctatgg taagtagtca gcataatacc
     1201 ataaaagcca ctctgcatct tcgttattta gcttaaaaaa cgcttgatta tgtgtgctac
     1261 cagtgttgcg aaaaagatct ttaagctttt ctgcgatgtc aatatcactc attacggttt
     1321 ctccttattc ttaatttggt ttatttgcta tgactgtgcc tagactgacg tcttattcat
     1381 attagtatga attgttttgc tatcattata gctttcgtct ttctctaatt atccgtcttg
     1441 caagagtaca gtggcagcta agtagtgttt tactttataa tgcggtatag atatcattaa
     1501 tttgacaggt atcttgtatg tagcgataat atgtatatct attacatact agaggtgtat
     1561 cactaatggt tagggtaaat attacgctgg acgaagagtt atataaggag ctttcgtcaa
     1621 tcaagaataa gagtgagttt ataagggctg cggtaagaga aaagcttagg ataataaagg
     1681 aagagaatct aaaaaaagtg cttaaggagg gttataaggg ggaaggaaag aacttgaagg
     1741 agtgggagtc aactgtagcc gatgcgtggg attaagagag gaagtgtgtg gttggtttct
     1801 gtagaaccta taataggacg agggataggg aagacaagac ctgcggtagt tatctcaaat
     1861 gacattaata acgagtatgc agagaccgtt acggtgattc caattacttc aagcgtatcc
     1921 aaggtctatc cttttgaagt gtttttatct aaagggaccg ctaacttgcc taaggactca
     1981 gaagcaaagt gtaatcagat aagaagtgtt gataaaagaa ggcttattag gcaaataggc
     2041 agggtttctc aacaaactat caaggaaata gaaaaagcac ttctaattca tctgggcttc
     2101 gaaatgtaca cctgaaacgc gtcttatcgc agtgagtagc tatcggcaag agttgggatg
     2161 cgaattaaaa tggatacgtg acaacggtgt tgattcagtt gaaactaatg ttatttatgc
     2221 tgtggggatt aagtgaataa agatttctaa tacttattct ttgaattacc gaaatgctta
     2281 agtcaaaagt caacgattgg ttcctttata cattcagtta taaatcaaat ttcttttatg
     2341 actattgtag taagattaat aacaataact ttcctaatag aataaaactt tattcaattt
     2401 tgaatagtta tgttgatctt caggaaagtg aaatattttc attgtattcg atttgttaat
     2461 agcaccttca aataaaaatt ctggttcctt ccctgttgga atataatata tataaaagat
     2521 cccgcttata aagaatttgt atattgttgt tttaccatct ttaaatcttt taggaggtat
     2581 tattaattgt tttacatcta attcttggtg ctttgcatgt agcaaaatac atgggtaatc
     2641 atgatgattt ttgggatcgg aattaataat catttttctt atttcttctc catgtaggcc
     2701 aagttttact tctttaaaaa aaacactatt agatatactt gatttccata aaatggataa
     2761 taaaaaaagt ttaaattttt cataagaaat gttttttaca acagtccatt ctaatactga
     2821 attttcagat ctttctcttc tatgttttat accagtagtt tcttcatttc cgtaataaac
     2881 tttcgaagca tagttttcat atctttgatt gatgatttta ttgtcacatt ccccacaaag
     2941 tatatctgac tcgtattcac cagttggctt tcttttaggt tttgattttc cccccaaagt
     3001 aatttgataa ataaagtgcc tttcatcata taattctttg tacataaaat caggaattat
     3061 atgagatttt ttaattaacg gtttttcttt taaacacaat ttacatttca tattgataga
     3121 tgttataaac attataagtt gattaaaatc ggattactta aattcaagtt ttgctgtttc
     3181 atatgtccat tttaatgcaa ccattctaat tatttctttt ttcatatatc tgaatacctt
     3241 tatgaatacc tttatagtga tatgcgaaac atacttgcta tttctcaaag gaaataacat
     3301 ttgcttttaa gctgcaatat cctatgaaga aaaagtgtcg tgtcttgatt cttaaatcaa
     3361 tcacttcgat gagcttaaat aatgcataat ctcctgccac tacacccata acatattttc
     3421 taggattatg catatgaatt tgagcaaaac aactaatccg attttaatcg caatcctttt
     3481 ggcacaacaa caagctccaa caaatcaaac aggccttgca cgagaagtgc tagaacggct
     3541 gcggggatag cgccttcgag aattagtccg acatcatcaa gtcggattcc tgtaagtatc
     3601 ggttggccgt atcctcctgc accgataagc gctccaagtg ttgcagtacc cacattaatt
     3661 accgcggagg ttttaatacc ggcaaggatt gagcgtgaag ccatgggtag ttctacaagt
     3721 cttagccgtg cacctgatgg taaacccaat gcctctgctg attcacgaat tcctacggga
     3781 atatcttgaa gtcctgtata agtattcctg acaatcggaa gtaagctata aagaaatagg
     3841 gctacaatag caggtgctga gccaattcca agtagtgga
//
LOCUS       DATDRL010000124         5991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1099818, whole genome shotgun sequence.
ACCESSION   DATDRL010000124 DATDRL010000000
VERSION     DATDRL010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5991
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099818"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VI935_04425"
     CDS             <1..1075
                     /locus_tag="VI935_04425"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLE24879.1"
                     /translation="VNFIKSIHPGFYIDFAYEYNFNRPQDGLNQLRVFDVEDNEFNLH
                     LFQLSFRRIPTTEGGLANLVGFGAKLDFGEDADVIGATGLGDADDEFDLQEAYIHILA
                     PFGNGIDIYGGKFVTLAGAEVIESKDDFNFSRSFLFGFAIPFTHTGIRIHYPIAPFDF
                     IVGLNNGWDVVDDNNDGKTIETRVGLNLEKFSIGVVGYFGPEENKVDGDWRELIDVVA
                     TLIPLENLTLVANLDFGWQQDANFDLDGDEITDVEENVFWWGVAGYIVYDLNEIVRLV
                     LRGEYFDDQDGFRTGTAQKLWELTPTLQLRPFAKYSPFDNLLLRLEYRHDQSDEDVFE
                     DVNGDFKKTQDTVAIELLYYFTL"
     gene            1460..2290
                     /locus_tag="VI935_04430"
     CDS             1460..2290
                     /locus_tag="VI935_04430"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HLE24880.1"
                     /translation="MFRKIYFLALVAVFVIPVIKPEGEEKLTLWEVYNQHIRPKKFVD
                     LTHAFYPGIPHWKGFPDEKRETLFWYEPGVGKLGSGFFAESFQHVGQWGTHVDPPAHF
                     IKGLRTIDEIDPEEMVMPLVIIDVHKKVEKNPDYQITMEDIKEWEKKYGSIPQGSFIA
                     MRTDWSKRWPSQDAMQNKDKNGISHYPGWSMGVLKYLFEQRKVTAIGHEPTDTDSGVA
                     TSNDDYSLETYVLSTNHYQIELITNLDQVPVAGALVIVSFPKPKGGSGFPARVFAILP
                     "
     gene            2463..2684
                     /gene="xseB"
                     /locus_tag="VI935_04435"
     CDS             2463..2684
                     /gene="xseB"
                     /locus_tag="VI935_04435"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HLE24881.1"
                     /translation="MKSFEGALDELKVIIEKLEGGKLSLEDSLTLFEKGTQLINLCHK
                     KLNEVEKKIEILVEGTNGEVTRKEFDLEE"
     gene            2689..3582
                     /locus_tag="VI935_04440"
     CDS             2689..3582
                     /locus_tag="VI935_04440"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HLE24882.1"
                     /translation="MRPSIELYLSTLKEIIDKKLVELLPNSPEPISKLQESMRYSILA
                     GGKRLRPILCIFASEAVGGSVDKALPIACAIEMIHTYSLIHDDLPSMDDDLLRRGIPT
                     NHSVFGEALAILAGDALLTDAFSLIGNEGISNGLAPKVVVEIIRDISKAAGSQGMVSG
                     QALDLAIEGKEVSLEFIERVHSLKTGALIVASVTSGARVGGASEDQLLQLKCYAKYLG
                     LAFQIIDDVLDIEGGAEIGKTIGADARKNKATYPLLVGVERARKVALELTEKAVLELR
                     DFDERAEPLRELAFYLGGRKY"
     gene            3566..4315
                     /locus_tag="VI935_04445"
     CDS             3566..4315
                     /locus_tag="VI935_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HLE24883.1"
                     /translation="MVGSIRSKERIDLLLVKKGLAPTRTRARALIMAGVVFVNGARVD
                     KAGTLVEEDAEIVLKDFALGYVSRGGIKLEAALKKFKIDVSDKVVLDIGASTGGFTDC
                     LLKHGAKKVYAVDVGYGQLDWKLRQDPRVVVMERINARYLKPENIGELVDVSTIDVSF
                     ISLTKVIPSVSKILKPGGILIALIKPQFEVGKGEVGKGGIVIDENKHGEVVNRITYFL
                     EELNFKVLGVIPSPILGADGNKEFLVGGVLL"
     gene            complement(4336..4980)
                     /gene="fsa"
                     /locus_tag="VI935_04450"
     CDS             complement(4336..4980)
                     /gene="fsa"
                     /locus_tag="VI935_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HLE24884.1"
                     /translation="MKFFLDTGNLDEIRDAASLGVLDGVTTNPTLVAKEGEQKGFKEL
                     IREICEIVKGPVSAEVISTDIERMLEEARELARIHPHVVVKMPLTEDGIRATKKVSEE
                     GIKVNVTLVFSPSQALLAAKAGATYVSPFVGRLDDVSNIGMDIVRDIVGIYKNYGYPT
                     QVLVASIRHPIHVVEAARAGAHIATMPHKVFKQLVKHPLTDVGLERFLSDWGKK"
     gene            5265..5555
                     /gene="rpsT"
                     /locus_tag="VI935_04455"
     CDS             5265..5555
                     /gene="rpsT"
                     /locus_tag="VI935_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HLE24885.1"
                     /translation="MAKRIRSAIKKHRQSLKKRLRNVHMLTLLKTLVKAVNNAVEAKD
                     LTKSLEALKIAEVTFKKAASKGMIHKRTASRNVSRLSKKVYELSKSMAQPTT"
     gene            complement(5571..>5991)
                     /locus_tag="VI935_04460"
     CDS             complement(5571..>5991)
                     /locus_tag="VI935_04460"
                     /inference="COORDINATES: protein motif:HMM:NF044544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24886.1"
                     /translation="NKISAEDVERLTEKEQFEDVFQLVNAIAEKNKKKALNALLDLEA
                     TKEEPLVILNKISWRFRSIWRAKELIDKNTPQDEIIKKLRTSSKAFYYIRQEARNFSY
                     EDIKRISRILYVGDRTLKTAYIPKNLTLTKLVLELCS"
BASE COUNT         1772 a         1074 c         1347 g         1798 t
ORIGIN      
        1 agttaatttt ataaagagca tacatccggg tttttatatt gatttcgctt acgaatataa
       61 cttcaataga ccccaggatg ggttaaatca acttagggta tttgatgttg aagataatga
      121 gttcaacctt catcttttcc aactctcttt tagaagaatt ccaacaactg aagggggctt
      181 agctaatctg gtagggtttg gcgctaagct cgatttcgga gaggatgcag acgttatcgg
      241 agcaacaggt cttggagatg cagatgatga attcgatctt caagaggctt atattcacat
      301 ccttgcaccc tttggaaatg ggattgatat ttatggtggt aagtttgtca cccttgcagg
      361 tgcagaggtg atagagagta aggacgactt caacttttcg cgctcgtttc tttttggctt
      421 cgctattccc tttactcata cggggattag aattcattat ccaattgccc cttttgattt
      481 tatcgtcgga ctaaataatg gatgggacgt tgttgatgat aataacgatg gtaagacgat
      541 tgaaacaagg gtaggactaa accttgagaa gttttcaata ggggttgtgg gttattttgg
      601 tccagaggaa aacaaggttg atggagattg gcgtgaactt attgatgttg ttgctacgtt
      661 aattccgtta gaaaatctga cccttgttgc aaatcttgac tttggatggc agcaggatgc
      721 aaattttgat cttgacggag acgaaataac tgatgtagag gagaatgtat tttggtgggg
      781 ggtcgcaggc tacattgtct atgaccttaa tgaaatagtt agattagtat taaggggtga
      841 atacttcgat gaccaagatg ggttcagaac tggaactgcg caaaagctat gggaattaac
      901 accgacccta caattaagac cctttgctaa gtattctcca ttcgataatt tacttcttcg
      961 tttagagtac cgtcatgatc agtctgacga agatgtattt gaagatgtca atggagattt
     1021 taagaagact caagatacag ttgcaataga gcttctttat tatttcactt tatagaatta
     1081 ataaaacccc atcttctctt catacctccc ttgataggac agccctgccc cattacgagg
     1141 ttggctgtcc taccattatt tttaccttag cttaaagaat taaagacata tcatagtttt
     1201 acttgaatgc gattaaaacc aaactttctg atttttgata ttgcataacg aaccccgttg
     1261 ctgcgaggaa cgaggtgacg aagcgatttt gggatttgcg gatttaaaaa ctttttatat
     1321 tcaacaatcc gcattccgca atccgaaatc ggcagtacgt tcctaaatac aactcttttt
     1381 gaatgcacta atttttgata gaattggtat aagaaggggt atgtaaagct gtgctactaa
     1441 gaagaggagg ttaaaagcca tgtttaggaa aatctatttc cttgctttag tagcggtatt
     1501 tgtgatacct gtcattaaac ccgaaggcga agagaaactt acgctgtggg aggtttataa
     1561 tcagcacata agaccaaaga aatttgtaga cctaacccat gctttctatc cagggatacc
     1621 tcactggaag ggattcccag atgaaaaaag ggagacgctc ttttggtatg aacctggagt
     1681 tgggaaattg ggctctggtt tttttgccga atcgttccag catgtaggtc aatggggaac
     1741 ccatgttgac ccgcccgctc atttcattaa gggtcttaga accattgatg agattgatcc
     1801 agaagagatg gtaatgccac ttgttataat tgatgtgcat aagaaagttg agaagaaccc
     1861 ggactatcaa atcacgatgg aagatattaa agagtgggag aaaaaatatg gctctattcc
     1921 tcaaggatcg tttattgcga tgagaacgga ttggtcaaaa aggtggccaa gtcaagatgc
     1981 gatgcagaat aaagacaaaa atggtatctc tcactatcct ggatggagta tgggagttct
     2041 aaaatatctg tttgaacaaa ggaaggtcac tgcaattggg catgagccta cagataccga
     2101 ttcaggtgtt gccacaagca atgatgatta ttctcttgaa acctatgttc tctctacaaa
     2161 tcattatcag atagagctta ttactaacct ggatcaggtg ccggtggcag gagcacttgt
     2221 aattgttagc tttccaaaac caaaaggtgg gtcgggtttt cctgcaaggg tatttgcgat
     2281 cttgccgtaa cgagtaaaaa catagccaca gagtacacag agaaaagaat gagaaatgtt
     2341 ctctctctat gtgctcagtg ttctctgtgg cggaatccct ttattctgaa atccgaaatc
     2401 tcaaatccgc aattgataag tctccttgtg taccttaaat tacaaggcta aaataactac
     2461 atatgaaatc atttgaggga gccctagatg agctcaaggt tattattgaa aagcttgaag
     2521 ggggcaaact ctctttagaa gattcgttga ccctttttga aaaggggact cagcttataa
     2581 acctctgtca taagaagctt aacgaggtcg aaaagaagat cgaaatttta gtagaaggca
     2641 caaacgggga agttacacgg aaggaattcg atttagaaga atagttttat gagaccaagt
     2701 attgaactct atctttcaac cctaaaagaa ataatagata agaagttagt tgagcttctc
     2761 ccaaattctc ctgagccaat aagtaagtta caggaatcta tgcgttatag tatcttagct
     2821 gggggtaaaa ggctaagacc tatcctttgt attttcgctt cagaagctgt aggtggatca
     2881 gtagacaaag ccctccccat agcatgtgca attgaaatga ttcataccta ctcattaatc
     2941 catgacgatc ttccttcaat ggatgacgat cttctaaggc gggggatacc aacaaatcac
     3001 agtgtgttcg gagaagcatt agccattctt gcgggcgatg ccttactaac agatgcattt
     3061 tcattaattg gtaacgaagg gatttccaac gggctagctc ctaaagtggt tgttgagatt
     3121 atacgagata tatcgaaggc tgcaggctct caaggaatgg taagcggtca ggctcttgac
     3181 cttgctattg agggaaaaga ggtgagcctt gagtttattg agagggtgca ctctcttaaa
     3241 actggggctt tgattgtggc atccgttaca tctggcgcaa gagtaggcgg agcaagtgag
     3301 gatcaactgt tacaactcaa gtgttacgca aaatatctcg gacttgcttt tcaaattata
     3361 gatgatgttc ttgatatcga agggggagcc gaaataggga agaccatagg ggccgatgcc
     3421 cgtaaaaata aggcaactta ccctttgtta gtcggggttg aaagagcaag gaaggtggca
     3481 cttgagctta ctgagaaagc cgttcttgaa ttaagagatt ttgatgagcg ggcagaacct
     3541 ctgagggaac tggcctttta tcttggtggt aggaagtatt agaagtaaag aaagaattga
     3601 tttgctcttg gtaaaaaagg gtttagcacc taccagaact cgtgcacgtg ctcttataat
     3661 ggctggagtt gtgtttgtta atggcgctag agtggataaa gcgggaacgc ttgtggaaga
     3721 agatgcagaa attgtactta aagattttgc tctaggatat gtaagccgag gaggaattaa
     3781 actagaagca gctcttaaga aatttaagat cgatgtgagt gataaagttg tgctagatat
     3841 tggagcttct acaggtgggt ttacagattg ccttcttaaa cacggagcaa aaaaggtcta
     3901 tgcagttgat gttggatatg gtcaattgga ctggaaacta agacaagacc caagagtagt
     3961 ggttatggaa agaattaatg ccagatattt aaaacctgag aatattggcg agttggtaga
     4021 tgtttctacc atcgatgttt cttttatttc tttaacaaag gttattccat cagtctcgaa
     4081 aatacttaag ccaggcggga ttctcatagc tttgataaaa ccacagtttg aggtagggaa
     4141 gggagaagta ggaaagggcg gtattgtaat agatgaaaac aaacacgggg aagtagttaa
     4201 tagaatcact tatttcctag aagaattaaa ttttaaagtt ctgggcgtta ttccttcccc
     4261 aatacttggt gcggatggga ataaggaatt tctagtagga ggggttttac tgtaggggca
     4321 attcatgaat tgcccctact tcttccccca atccgagaga aacctttcga gtccgacatc
     4381 agttagagga tgtttaacaa gttgcttaaa tactttatga ggcattgtgg caatgtgagc
     4441 tccggctctt gcggcctcga ctacatgaat cggatggcga atgcttgcga ccagaacttg
     4501 ggtggggtag ccatagtttt tataaattcc aacaatgtct cttactatgt ccattcctat
     4561 atttgagaca tcgtcaagcc ttccaacaaa tgggcttaca taggtcgctc ctgcttttgc
     4621 tgcgagtagc gcctgagagg gtgaaaagac taaggtgaca ttaactttaa tcccttcttc
     4681 agataccttt ttcgttgctc taatgccatc ttcggttagg ggcattttta ctaccacgtg
     4741 agggtgaatc cgagcgagct cccttgcttc ttcaagcatc ctttctatat ctgtacttat
     4801 tacttccgcg cttacagggc cttttactat ctcgcatatc tctcttataa gttctttgaa
     4861 tcctttctgc tcaccctctt ttgctacaag tgttggattt gttgtaactc cgtccaggac
     4921 tccaagacta gcagcgtctc ttatttcatc taagttgcca gtatcaagaa agaatttcat
     4981 tgctaaagca ccttttctta gcttgaacta taccatgatt tagcaggtga gtgaagaaga
     5041 ggaggtttaa gattgtctgc ttgtcatagc gagggtcgaa gtggcgatgc aataacttag
     5101 agattcctca ctacgttagg aatgacagat agtaggggcc tcttgcacca acaccattgt
     5161 ctggccaacc caagggttgt ccagtattgc cataacaata tgtatttgac aaatcctcaa
     5221 ttaacagaaa actatttttt taatttaata tcgaggggta gctaatggct aaaagaattc
     5281 gttcggctat aaagaagcat cgtcagagtc taaagaaaag gcttagaaat gtacacatgc
     5341 ttactcttct aaaaaccctt gtaaaagcgg ttaataatgc tgttgaagct aaggatctaa
     5401 caaaatctct tgaagcctta aagatagccg aagtaacatt taaaaaggct gcctcaaaag
     5461 gaatgattca taagcgaact gcttcaagga atgtctcgag gctttcaaag aaggtttatg
     5521 agttatctaa aagtatggca caacccacaa cttgagagta ccagcttaaa ctagctacag
     5581 agttccaaaa cgagttttgt gagagtaaga ttttttggga tataagctgt ctttaacgtt
     5641 ctatcaccta cataaagaat cctactgatc cgcttaatat cttcgtagga aaaattcctt
     5701 gcctcttgcc gtatgtaata aaatgctttc gacgaagttc taagtttctt tatgatctcg
     5761 tcctgtggcg tatttttatc aataagctct tttgctctcc aaattgatct aaatcgccaa
     5821 cttattttat ttaagataac aagtggttct tcttttgtcg cttcgagatc taaaagagcg
     5881 ttcagtgcct ttttcttatt cttttcagca attgcgttta caagttgaaa tacatcttcg
     5941 aattgctcct tttcggtaag cctctctaca tcttcggcag agattttatt t
//
LOCUS       DATDRL010000125        15013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2144269, whole genome shotgun sequence.
ACCESSION   DATDRL010000125 DATDRL010000000
VERSION     DATDRL010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15013
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2144269"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VI935_04465"
     CDS             complement(<1..168)
                     /locus_tag="VI935_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HLE24887.1"
                     /translation="MNIYVGNLTRDVTDEDLRKLFEEFGQVSSVNIIKDKFSGESRGF
                     GFVEMPGRKEAE"
     gene            complement(392..754)
                     /locus_tag="VI935_04470"
     CDS             complement(392..754)
                     /locus_tag="VI935_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24888.1"
                     /translation="MKQVLLLSITLIASIILFSEIKEVLGMASEEEEIIILIQTATSP
                     EDHLKIADYYDKQAKKDEEKARFYASMSDSYANRDKPLLGLAKNYSDQSKKYMEMAQG
                     YKNLATEHRKMAGQEMQR"
     gene            complement(969..1433)
                     /locus_tag="VI935_04475"
     CDS             complement(969..1433)
                     /locus_tag="VI935_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24889.1"
                     /translation="MKQPFEEFDKIKELDGQHKVCWEVLPEFYINQNGERVQIGFELD
                     LVGTHYHPVQSPIPGCKECVKVYEDLKQIAHWIMPKEERDSVYGIRPFDVSIHQAPLR
                     KFRKDITLAIKILHRDGFDRPKDACEVECLKEMEKKLKDLEAHQGYLWDIGR"
     gene            complement(1468..4695)
                     /locus_tag="VI935_04480"
     CDS             complement(1468..4695)
                     /locus_tag="VI935_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HLE24890.1"
                     /translation="MIEKTIEYFSRNRFIVFIIVGFLTVWGIWAVKHVPLDAIPDLSD
                     VQVIVFTEWPGRSPDLVEDQITYPIVTTLLSAPKVTVVRGTSMFGFSFVYVLFEDGTN
                     IYWARTRVLEYMSGIRGRLPEGVTPVIGPDATGVGWVFQYALVDETGKYNLAELRSFN
                     DWYLRYWLRSVPGVAEVESIGGFQKQYQVNVDPTKLLAYKMPLNKVIESIRMSNNEVG
                     GRVVEATGREYVVRGRGYIKSIQDIEDIVVAIDGRGTGTPVLIKDIADVSLGPDMRRG
                     IAELDGKGEVLGGVVVMRYGENALNVIKKVKEKLKEVEPAFPDGVKLVVTYDRSSLIL
                     RAIDTLKRTLIEESIIVSLVCILFLFHFRSALVIIFTLPFAILFSFIPMYYMKLTSNI
                     MSLGGIAIAIGAMVDAAIVLVENVHKRLEHWEEEGRIGDRLQVMIDASKEVGKPIFFA
                     LLVITVSFIPVFTLEAQEGRLFKPLAYTKTFGMFFAALLSITLTPALITILIRGKIGS
                     EKNNPISRFLIRVYEPLVKLVVRWKKTTVAVALVILLVTAPLFFKLGSEFMPPLNEGS
                     IMYMPITPPGISITEAGRLLQIQDKIIRWFPEVETVFGKIGRAETSTDPAPLSMVENT
                     ITLKPEEEWRKIPQRRWYSSWAPEFVKKLLRPIWPDERTMSWDELTAEMNSMLKFPGV
                     TNIWTMPIRNRTDMLTTGIRTPVGIKIFGKDLSVIEEIGKQIEAALRDVPGTISAYAE
                     RVTGGYYLDFNIRRKEAARYGLTVGDVEDIIEAAIGGNNITQTVEGPERYSVNVRYAR
                     ELRDDVEKLKRVLVPTPTGAQVPLEQLAEIQAQTGPSEIRNEDGSKAAYVFIDMGGRD
                     IGGYVDEAKRTVSEKVKLPQGYYISWSGQYEFLLRVREKLKLVIPITLLIIFVLLYLN
                     FGSVIETLIVILSVPFALVGSIWFMYLLDYNMSIAVWVGIIAVAGLAAQTGVVMIIYL
                     DNAYKKRVKDGRMNNWDDLAEAIDEGAVQRVRPKMMTVMTMILGLLPLLWSHGTGADV
                     MKRIAAPMVGGLVTSTILTLLIIPAIYATWKGGALKQHKDGKENG"
     gene            complement(4743..5108)
                     /locus_tag="VI935_04485"
     CDS             complement(4743..5108)
                     /locus_tag="VI935_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HLE24891.1"
                     /translation="MKIKSYRELDVWKKGIEIVDLVYLVTSKFPKEERYGLATHMQRT
                     AVSIPSNIAEGFSRQHTKEYQHFCYIALGSCAELETQLIIAQRRNYLSENDFANLEDY
                     IDHESRMLMSLIKSLNRGS"
     gene            complement(5192..6781)
                     /locus_tag="VI935_04490"
     CDS             complement(5192..6781)
                     /locus_tag="VI935_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLE24892.1"
                     /translation="MTKKNLVKLVAVVAITLLILILAFSYIFERPEGEGSSARHKTGG
                     EKSIVTQHSHEMPGIQQESGVETQLKDKQGPSELVGIEISPEKQQLIGIKTEEVVSRN
                     LVKHIRTVGIVEADERKIAHVHVKFSGWIKDMYVYEGSYVKRGEPLFTIYSPELVSSQ
                     EEYLLALKINETLNKSQYPEVSSGAISLLEATRRRFRLWDISEGELKEIERSGKPLTY
                     MTIHSHMNGYITKRMFFPGMHVAEHEDVLVMADLSDVWVIADIYEYELPLVKVGEVAT
                     LQLSYYPGETFTGKVMYIYPVLDTTTRTAKARFEFPNSENKLKPGMYANVMLKSDLGE
                     RLSISEDSVIDTGERKVVFIASGEGYFEPREIKVGKKAEGYYEVQNGLREGERVVRNA
                     TFFVDSESRLKAAFQSLSGGREELVAVGGSQASGKSDIKISFSTDPDPPESGKTTFKF
                     KLTDPEGKPITDAKVKFTILMPAMPGMAEMRSAGDAKHTGDGVYEGELNIPMSGGWAL
                     EIGALMPGKPPVNESFDLNVK"
     gene            complement(6890..8272)
                     /locus_tag="VI935_04495"
     CDS             complement(6890..8272)
                     /locus_tag="VI935_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLE24893.1"
                     /translation="MRLILAFILTIALTGVSAFAVVSDAQCASDGNKKLVSDAALSVP
                     QCGTDGNNECAQDENKDLTSLKSLVEEALRNNPEIKAGRARWEASTKRPSQVSTLPNP
                     TIGVRYMNVSFDRLTLGEDDFTEFGIISASQEFPFPAKLSLKGKIAKEEAEAEEEFSE
                     ATVKRVLADLKEAYYEWYLANKSIEITSKNKELIEKFVKIAEAKYAVGRGIQQDVIKA
                     QVELLGFVERLELLVQKKEVVEARLRSILGRTPDSPIGKPEEVQKSDLKLSLDELYKL
                     TESESPILLAKKNLIERNDQALQLAKKEYFPDFVVDFEWINRGEFTSDGPFADMWQVR
                     VGFIVPLYFWRKERFGVEEAVSQLVEMKEEYNNANLNLSFDVKDKYITAKTSEKLLDL
                     YSKGIIPQASLSLESAIAGYEVGNVDFLTLLDNLVTLFNYELAYYTQLAEYQKALARI
                     EENIGVVLVE"
     gene            complement(8387..8749)
                     /locus_tag="VI935_04500"
     CDS             complement(8387..8749)
                     /locus_tag="VI935_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24894.1"
                     /translation="MNKMSQIVIALIVFLMLFSFIGVVPGGASGEEDVDKMIETATTP
                     EDHMKIAEYYEKQAQKMEEKAGLHASLADSYKNRGKPFSALAKHCSDLAKKYMEVAKD
                     YKAMAMDHMKMAQEIQKQ"
     gene            complement(8937..9320)
                     /locus_tag="VI935_04505"
     CDS             complement(8937..9320)
                     /locus_tag="VI935_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24895.1"
                     /translation="MKYLSFFLALTTFLCYTGICADVSYSNNTSETTTNTSCHGTKKQ
                     DTSSSAISYKNSNHINHEIDMCQVVLPNAPQGYDLNQNHIILSSPAVYVPILEINKAS
                     VLVFSFNIKEHHPPELFLSNSSFLL"
     gene            9452..10279
                     /locus_tag="VI935_04510"
     CDS             9452..10279
                     /locus_tag="VI935_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLE24896.1"
                     /translation="MEIKKGLDYSTLHKLDSPANQLIRKSIWGDQDIGQQSFITPWYL
                     DELIRKTAIDANTYVLDIGSGVGGPAIYITEKTKCRIMGIDISDVGVERARKFAKESG
                     LSNRVTFHLGDAMEMPFPDSTFGVAISINVMNVFKDKKGLFQQVIRVLRPNGIFALLS
                     GTIDLPDDPEIIENMARGYLIPQYYDTVDGYKSKLKEVGFGILEVIEYVEDFRIQNKL
                     WGDAYKKHYDAIVKEQGKENTDYHIVYFDTYLRLIDEGRAGNHLFLSRKPGNFKNSK"
     gene            complement(10282..11691)
                     /locus_tag="VI935_04515"
     CDS             complement(10282..11691)
                     /locus_tag="VI935_04515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLE24897.1"
                     /translation="MRKYDIIVIGAGSGGLNIAGFMNRAGFKVLLIDKSDRRIGGDCL
                     NSGCVPSKALIHVARLLKNAKEASRFGLTVNGKTDIKKVLGYVKEKQEVIRVHENAEH
                     FRKIGMDVALGEARLTGPDLVTVGNETYRGKKIILATGSRPRKLNVPGIGNVRYETNE
                     TIFDLEKLPEHLLVIGGGPIGIELGQAFRHLGSKVTVVEMDSKFLPKESPEIADVLHR
                     RMVSEGVDFIFNATVKEFPSSNQAIIVNKDKKEIRLDFDTVLVSIGRDYNINGLSLEK
                     AGIELENNGKKLKVDEYLRTTNKRVFVSGDVAGSYQFTHAAEVHAGVIINNFFSPFKK
                     KLNNDHMAWVTYTSPEVATFGLNEEQLKQRGISYEKVVFDFEEDDRAIVDESHDGKLL
                     LFIAKDKILGGSMVAENAGELSQELILANSSGLKLKHIFGKVYPYPTASRVNKRIISL
                     YYANKLTPFAKRVLHLFYG"
     gene            11921..13036
                     /locus_tag="VI935_04520"
     CDS             11921..13036
                     /locus_tag="VI935_04520"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HLE24898.1"
                     /translation="MIGEKIKNLRESEGLTQEEVAVKVGVSKEFISMVESGKRNPSLE
                     VLSKIASVFSKEVSHFLQTPKEDFALALRTAEINEKDKDEIIKYKEFCEDYYSLEEVT
                     GEILQPLAPIYPDPSSSILSDFGQTYRYAEKLALDERRRLSLGDEPIKDIFLLVETQG
                     VRVVRINMVDSSVDGAFIYSRDKGAFMLINSFLSKGRQVFTAAHEYCHYLKDREKGYP
                     VCQIITGDIEEHKKPIERIANLFAANFLMPETTVSKLVGLYAKNRLEAEEVVYIKRYF
                     GVSYQAMLYRLKDLRFIRRPKLQEVLKTDPATLEIALFGFTEESVKDPERLPERYCKL
                     AVQAYTEKKISLEKLAELLKVDSVELRERLSKGGFIK"
     gene            13175..14293
                     /locus_tag="VI935_04525"
     CDS             13175..14293
                     /locus_tag="VI935_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLE24899.1"
                     /translation="MEHQIKEFTSPVLDKPLRKYKIHCPLHFFAFGTFKKVLLKNGYE
                     VAHPEGIFYHRPNRALIYPTIAGPHEYYTLMFLTTLDFKDLENLFFQAFNEDYKNNYV
                     LIEGIESCRVRNSLSGPHFDEIKHTLPQSVVTILENSLWKFEDFCNRFSRYNMSNTLG
                     MLFYGQPGVGKTYILRSYFNKLLLERNFTIVQVYQDCLDYMNMSVLLNSCQALFPCIL
                     FLEDIDVNFKDRREPANSLVGFLLETFEGLLNVEKVVLIATSNNVDMIEKALLRPGRI
                     DYLVELEKPSKSAKELVLSNYLNGSDLNLPDPLKELLINNVDTFAELKGGFQHLLRTY
                     VNKGDFPSMEEVSRLIRTWKEAKVVGGLENGERRMGLV"
     gene            14376..14624
                     /locus_tag="VI935_04530"
     CDS             14376..14624
                     /locus_tag="VI935_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24900.1"
                     /translation="MKTDLYTKIVLTIIAIALWGLLLKPTFVSQSVGASTQVLDVNIK
                     EIGGRAINDKILDVNIEKINGRTFTTTIPPALPVEIKK"
     gene            14749..>15013
                     /locus_tag="VI935_04535"
     CDS             14749..>15013
                     /locus_tag="VI935_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HLE24901.1"
                     /translation="MSKNLSKSKYLSGLQCEKRLWLEINDPDKASETPEYQQRLFNQG
                     KEVGIRARRYFEEGYLIDKDRLKIYECVEETRDAIARGESIIFE"
BASE COUNT         4294 a         3239 c         2807 g         4673 t
ORIGIN      
        1 ctcggcttcc tttcttccag gcatctcaac aaaaccaaat ccacgggact cgccgctaaa
       61 cttatcctta ataatgttta ctgatgaaac ctgtccgaat tcttcaaaaa gttttcgtaa
      121 gtcctcgtca gtaacatctc gtgtcaaatt accaacgtat atattcatac ttccctcctt
      181 tattcatact tacctaattt atattgaaag cctcatcgat ctctttctta tcataaggta
      241 tcaagataaa ctctgaatta acagcttaca cgaaatattt atgatttaca taccccgaat
      301 taaataaacg aattttccag ttccctaaca cattcatagt tgaccctacc ctatcatgga
      361 gggacgcctc cgtgcctgaa taggaagtta attatctttg catctcttga ccagccattt
      421 tcctatgctc tgttgccaag tttttatatc cttgagccat ttccatgtac ttctttgatt
      481 gatctgagta attctttgca agcccaagta atggcttatc cctatttgcg tatgagtcgg
      541 acatagaggc ataaaaacgt gccttttcct cgtccttttt agcctgctta tcataataat
      601 ctgcaatttt taggtgatcc tctggagacg tagctgtctg tataagtata ataatctcct
      661 cttcctcaga agccatgcca aggacttctt ttatttcaga gaaaagaata atagaagcta
      721 tcaaagttat gcttaacagt aatacttgtt tcatcactat accctcctct aatttaataa
      781 ttccatttag cttatttcac gattcttcat caatatttga tctatcaatg atagcattcc
      841 ttcctatcct taggactata aagttttact ccatccgtat tggtatcata cagtaaggaa
      901 atggtaaatc aaagtttaca tttttgccct ttatatgaag ttgactaaaa cgatgattta
      961 agaatctact atctaccaat atcccacaaa tacccctggt gtgcttctag atctttaagt
     1021 ttcttctcca tctctttgag acattctact tcgcaggcat ctttgggtct gtcgaaccca
     1081 tcgcgatgta gaatctttat agcgagtgtg atgtcttttc taaattttcg cagaggagct
     1141 tggtggatcg atacatcaaa aggtctgatt ccatatacgc tatcacgttc ctccttgggc
     1201 atgatccaat gtgcaatttg ttttaggtct tcatatactt tcacacattc cttgcaaccc
     1261 ggtataggtg attgtaccgg atgataatgt gtacctacaa ggtctaattc aaaaccgatc
     1321 tgtactcttt caccgttttg gtttatataa aattctggca atacctccca acaaacttta
     1381 tgctgcccgt cgagttcctt gattttgtca aattcctcaa aaggttgctt cattgtatat
     1441 tacctccctc attccctgga tttgctctta accattctct tttccatcct tatgttgttt
     1501 caatgcccca cccttccatg ttgcgtatat tgcagggatt ataagaagcg taagaatagt
     1561 tgatgtcaca agtcctccaa ccataggggc tgcgattcgc ttcattacat cggctccggt
     1621 gccgtgactc caaagaagtg gaagaagtcc taagatcatc gtcattactg tcatcatctt
     1681 gggtcttacc ctctgcacag caccctcatc tatcgcttca gccaaatcgt cccagttgtt
     1741 catccttccg tctttcactc tctttttata ggcattatca agataaatta tcatcacaac
     1801 cccagtttga gctgcaagcc ctgcaactgc tataatacct acccagactg ctatactcat
     1861 gttgtaatca agaagataca taaaccaaat gctcccaacc aacgcaaatg gtacagagag
     1921 aatgacgatt agtgtttcaa taacgctacc gaagttaaga taaagcagaa cgaagattat
     1981 gagaagcgta ataggaatta ctaattttaa tttctccctg acccttagga gaaattcgta
     2041 ttgaccgctc cacgaaatgt aatagccctg cggaagtttt accttttcac tcactgtacg
     2101 tttagcttcg tcaacgtacc ctccgatatc tcttcctccc atgtctatga atacataagc
     2161 agccttggag ccatcctcat ttcttatttc agagggtcct gtctgcgcct ggatctcagc
     2221 tagttgttca agggggacct gtgcccccgt gggtgtagga acaagtaccc ttttgagctt
     2281 ctccacatca tcacggagtt cccttgcata tctaacattt accgaatacc tctcaggtcc
     2341 ctctacagtt tgtgttatgt tatttcctcc tatagccgcc tcgattatgt cctctacatc
     2401 cccaacagta agtccataac gtgctgcctc tttccgtcga atattaaaat cgagataata
     2461 cccccctgtt accctttctg cataagcact tatcgtacct ggaacatccc ttaacgcagc
     2521 ctctatttgc tttcctattt cttctattac gcttaggtct ttcccaaaga tttttatccc
     2581 tacaggagtg cgaattccgg ttgtaagcat atcggttcta tttcttatag gcatagtcca
     2641 tatattcgtg acgcctggga atttaagcat actattcatt tcagcagtta gttcatccca
     2701 tgacatggtt ctttcatcgg gccagatagg acgtaggagc tttttaacaa actcaggtgc
     2761 ccagcttgaa taccaccttc tttgtgggat ctttcgccac tcctcctcgg gctttagggt
     2821 tatggtgttt tcaaccattg aaagcggtgc ggggtcggtt gaggtctcag ctctacctat
     2881 ttttccaaaa actgtttcaa cctcagggaa ccatctaatt atcttgtcct gaatctgaag
     2941 aagccttcct gcctctgtaa ttgatattcc tggaggggtt ataggcatgt acattatgct
     3001 cccctcatta agcggaggca taaattcgga acctagctta aaaaataggg gagctgttac
     3061 taataagata acaagagcaa cagcaacagt tgtctttttc caccttacaa cgagttttac
     3121 taaaggctca taaacacgga ttaaaaaacg gcttattgga ttattcttct ctgagccaat
     3181 ctttcccctg ataagaatag ttatgagggc aggtgtaagg gttatagaaa gaagagcagc
     3241 aaagaacatt ccaaatgtct tagtgtaggc aaggggctta aatagtctcc cttcttgggc
     3301 ttctagtgta aaaacaggaa tgaatgaaac tgttataaca aggagcgcaa agaatattgg
     3361 ttttcctacc tcttttgagg catctatcat tacttgtaat ctatccccaa ttcttccctc
     3421 ctcttcccag tgttctagcc tcttatgcac attctcaaca agtacaattg cggcatcaac
     3481 catagcccct attgcaatgg cgattccacc tagcgacata atgtttgatg tgagtttcat
     3541 gtagtacatt ggaatgaatg aaaatagtat tgcgaaaggc agtgtaaaaa taattacaag
     3601 cgccgacctg aaatgaaaaa gaaagagtat gcaaacaagg cttacgatta tgctttcttc
     3661 aattagtgtt ctctttaaag tgtctatcgc tcttaaaatc aggcttgatc tatcataggt
     3721 tacaactagt ttaacaccgt caggaaaagc aggctcaact tccttgagtt tctcctttac
     3781 cttttttata acgttcagcg cattctcacc gtatctcatt accacgacac cacctaaaac
     3841 ctctcccttt ccgtcgagct ctgcaattcc gcgcctcata tcgggaccca agctcacgtc
     3901 ggctatgtct tttataagta ctggtgttcc tgttcccctt ccatctattg ccacaacaat
     3961 atcttctata tcctgtattg atttgatata tcctctgcct cttacaacat attcccttcc
     4021 tgtagcctct actactcttc cacccacttc attattgctc atccttatgg actcaataac
     4081 cttgttcagt ggcattttat atgcaagaag cttcgttgga tctacgttta cctgatactg
     4141 cttttgaaaa cccccgatac tttcaacctc tgcaactccc gggacacttc taagccagta
     4201 tcttaagtac cagtcgttaa acgaacgtag ttctgccaaa ttatacttac cagtttcatc
     4261 tacaagtgca tactggaaaa cccatccaac acccgttgcg tcgggcccaa ttacgggagt
     4321 aaccccttct gggagtcttc cccgaattcc actcatatat tcaaggacgc gggttcttgc
     4381 ccagtatata ttggttccat cttcaaatag cacataaacg aaggagaaac cgaacatcga
     4441 tgttcctctt acaactgtga cttttggtgc tgaaagaagt gttgtcacta tggggtatgt
     4501 aatctggtct tctactagat ctgggcttct tccaggccac tctgtaaaga ctatcacctg
     4561 gacatctgaa aggtccggta tcgcatcaag gggcacatgc tttacagccc atatgcccca
     4621 gacggtaagg aacccaacta taatgaaaac tataaatcta tttcgagaga agtattcaat
     4681 cgttttttcg atcattttca cctcgttgat cgtggttcgt gattcatggt tcgatataat
     4741 tgtcacgaac cccgatttaa actctttatt aaactcatga gcatccgact ctcatggtct
     4801 atataatctt ccaaattagc aaagtcgttt tcggacaaat agtttcgacg ctgtgcaata
     4861 atcaattgtg tttccaattc cgcgcatgaa cccaaggcta tgtaacaaaa gtgttgatat
     4921 tccttagtat gctgtcggga aaatccttct gctatgttag atggtataga caccgctgtc
     4981 cgttgcatat gtgttgccaa tccgtatctt tcctccttag ggaatttact tgtaactaga
     5041 taaacaaggt caactatttc aatccctttc ttccaaacgt ctaattctct atagcttttt
     5101 atcttcattt tcgtgtttag tgaatcgtga ttcgtggctc ttggttgctt actggattca
     5161 cgagtcacga gccacgagcc acgagccacg attattttac attcaaatca aaactctcat
     5221 tcacaggcgg ttttccgggc ataagtgctc caatctctag agcccaacct ccgctcatag
     5281 gtatatttaa ctctccttca taaaccccat ctccagtatg ttttgcatca cccgcgcttc
     5341 tcatttcagc cattcctggc attgcaggca ttaggatagt gaacttgacc ttcgcatctg
     5401 taattggttt accttctgga tcggtgagtt taaacttgaa ggtggttttt cctgactcag
     5461 gcggatccgg gtctgtagag aatgaaatct taatatcact ttttccactc gcttgacttc
     5521 cgccgaccgc aaccaactcc tctctccctc cacttagaga ctgaaacgct gctttaaggc
     5581 ggctctcgga gtctacgaag aacgtagcat ttctaactac tctttctcct tcccgtagcc
     5641 cattctgtac ttcataataa ccctcagcct ttttccctac ttttatttcc cttggttcaa
     5701 agtatccttc tcctgaggca ataaatacaa ccttcctctc tcctgtatca attaccgagt
     5761 cctcagatat agagagcctt tctcctaggt cgcttttgag cattacgttt gcatacattc
     5821 cgggcttgag tttgttctct gaattaggaa actcaaatct cgcctttgca gttctagtgg
     5881 ttgtatcaag cacaggatat atatacataa cctttccggt aaaggtttca cccggatagt
     5941 aggaaagctg aagtgttgct acttctccca ccttaactaa cggaagttca tactcataaa
     6001 tatctgcaat aacccatacg tctgataggt ctgccataac gagcacatct tcatgctccg
     6061 ctacgtgcat tcctgggaag aacatcctct tagtgatgta accgttcata tgggaatgta
     6121 tggtcatgta tgtaagaggc ttgccactcc tctcaatctc ttttaactcg ccttcagaaa
     6181 tatcccagag acggaacctt ctccgtgtag cctcaagtag agagatcgca ccggaggaca
     6241 cttcaggata ttgactttta tttagtgttt catttatctt tagagcgaga agatattcct
     6301 cctgactcga aaccaattca gggctgtata tcgtaaaaag cggctcacca cgttttacgt
     6361 atgacccctc gtagacatac atgtctttta tccaaccgct aaacttaaca tgcacatgag
     6421 ctatttttct ctcatccgcc tcgacaatac ctacggttct aatgtgtttt acaagatttc
     6481 tagaaacaac ttcttcggtc tttataccta ttagttgctg tttctcaggg cttatttcga
     6541 ttccaaccag ctcagatgga ccttgtttgt ccttaagttg tgtttcgaca ccggactctt
     6601 gctgaatacc tggcatctcg tgcgaatgtt gtgtaactat gcttttttct ccacctgtct
     6661 tgtgcctagc cgaactacct tctccctctg gtctttcgaa tatgtacgag aaagcgagaa
     6721 tgagaataag caaggttatg gcaaccacag ctacaagttt gactaaattc tttttcgtca
     6781 tgttataccc ccttgctaga tgcacaatat acaatgcatg attctggaat gtttttgcat
     6841 gcgtcttgca tcatgtatcc tgtatcttgc atcctgaatc ctgattattt tattccacta
     6901 aaaccacccc gatattctcc tcaatcctag ccaaagcctt ttgatattct gcgagttgag
     6961 tgtagtatgc gagctcgtaa ttaaagagtg tgacaagatt gtctaggagt gtaaggaaat
     7021 caacgtttcc tacctcatat ccggcaattg ctgactcaag tgagagagaa gcttgtggaa
     7081 ttatcccctt agagtaaagg tctaacagtt tctccgatgt cttggcagta atgtatttat
     7141 cttttacatc aaaagataaa tttaagtttg cattattgta ttcttctttc atctctacga
     7201 gctgagatac agcttcttca actccgaacc tttcctttct ccaaaagtaa agtggtacga
     7261 taaagccaac ccttacctgc cacatatcgg caaagggacc atcgctggta aactctcctc
     7321 gattaatcca ttcaaaatct actacgaaat cggggaaata ctctttcttc gcgagttgta
     7381 aagcctgatc gtttctttca ataagattct tttttgccag aaggattgga ctctcacttt
     7441 ctgtaagctt gtagagttcg tctaaggaca atttaaggtc tgatttttgt acctcctctg
     7501 gtttcccaat aggagaatcc ggtgtacgac ccaggatgct tcttaacctt gcttctacaa
     7561 cctccttctt ctgcacaaga agctcaagcc tttcaacaaa ccctaaaagc tcaacctgag
     7621 cttttattac atcctgttgt attccccttc caacagcgta tttggcttcg gctatcttta
     7681 cgaatttctc aataagttct ttattctttg aagtaatttc tattgattta tttgctagat
     7741 accattcata ataagcttcc tttagatcag ctaataccct ttttactgta gcttctgaga
     7801 actcctcctc agcttcggct tcttctttcg caatctttcc cttaagtgaa agtttcgcgg
     7861 gaaatggaaa ctcttgagag gcagaaatta ttccgaattc tgtaaagtca tcttcgccaa
     7921 gtgtaagtct gtcaaaactg acattcatat atcgaactcc tatagtaggg tttggaaggg
     7981 tacttacttg agacgggcgt ttagttgatg cctcccatct agctcttcct gcttttattt
     8041 ccgggttatt gcgcaatgct tcctcaacaa gtgatttaag gcttgtaagg tctttatttt
     8101 catcttgtgc acactcatta ttaccatccg ttccacactg cggaacggat aatgcagcat
     8161 cagatactag ctttttattt ccatctgatg cacactgcgc atcagatact acggcaaaag
     8221 cactaacccc agttaaggct attgtaagta tgaatgcgag aatgagtctc atctttacac
     8281 ctccttatag aaacctctgc ggttcttgcg atatagagtg accttataaa tgccataagt
     8341 tccccaagca ggagaggttt cttccatgcc tggggaatca tttatatcac tgcttttgta
     8401 tctcctgagc catcttcata tggtccattg ccatagcctt gtagtctttc gcgacttcca
     8461 tatacttctt tgcaagatca gagcagtgtt tagcaagtgc cgaaaagggc ttacctctat
     8521 tcttgtatga atcggctaag gaggcgtgga gacccgcctt ttcctccatt ttctgggctt
     8581 gcttctcata atactctgca attttcatat gatcctcagg tgtcgtggca gtttctatca
     8641 ttttatctac atcttcctcc ccactagcac ctccaggaac cactcctatg aaagagaaaa
     8701 gcataaggaa tactatcaaa gctataacta tttgtgacat cttgttcatt tttgatacct
     8761 cctttctttt tataagcgaa ttactatgtt taatcattgc ttgtaacctc cctcttaagg
     8821 ttttataaaa ggatttattt ggcactttac ccctgcctga aagtgtgaaa acgatataaa
     8881 gatagtgata ggcttttaga gctatacact aagcagaggt tagaccagag tggatattaa
     8941 agtaagaagg aagaattaga tagaaataac tcaggtggat ggtgttcctt aatgttaaaa
     9001 ctaaagacaa gtacagaagc tttattaatt tctaaaatag gcacataaac agccggtgat
     9061 gataaaataa tatgattttg atttaaatca taaccttgtg gagcattagg aagtacaact
     9121 tggcacatat ctatttcatg gtttatatga tttgagttct tgtaggaaat tgctgagctg
     9181 gaagtgtctt gctttttagt tccgtgacaa ctcgtatttg ttgttgtctc gctagtatta
     9241 tttgagtaac tgacatctgc acaaatgcct gtgtagcaga gaaatgtggt tagggctaag
     9301 aaaaagctta gatatttcat gctcgtttaa atatagcttg attaatctga aaagtaaata
     9361 aattaaaaaa attttataaa aaatgtctta tgcgtattct tggaattgtt gaattatcta
     9421 atagaatgag taagttaaag gagaggagaa aatggaaata aaaaagggac ttgattactc
     9481 aacgttacat aaactagata gtcctgccaa tcagttaatc agaaaaagta tctgggggga
     9541 ccaggatatt ggacaacagt cttttatcac accgtggtat cttgatgaac tgatccgtaa
     9601 aacagcaata gatgctaaca cctatgtatt agatatcggg agtggtgtag gtggaccagc
     9661 tatctacata actgagaaaa ccaaatgccg cataatgggg atagatattt cagatgttgg
     9721 agtagagcga gcaagaaaat ttgcgaagga atctgggctt agtaaccgtg taaccttcca
     9781 tctcggtgac gctatggaaa tgccatttcc agacagcacc tttggtgtgg caattagcat
     9841 taatgtaatg aatgtgttca aggacaaaaa gggacttttc caacaggtca ttcgtgttct
     9901 aagacctaac ggtatttttg ccttattaag cggaactata gatttgccag atgaccctga
     9961 gattatagag aacatggctc gcggctatct tattccacaa tactatgata cggtagatgg
    10021 ttataaatct aaactcaagg aagttggatt tggcatactc gaggttattg aatacgttga
    10081 ggattttaga attcaaaata agttatgggg cgatgcctat aaaaaacatt acgacgccat
    10141 cgtaaaggag caaggcaaag aaaacaccga ctaccatatt gtctactttg atacctatct
    10201 tcgccttatt gacgaaggcc gtgccggaaa tcacctcttt ctgtccagaa aacctggcaa
    10261 cttcaaaaat tctaagtaaa ctcatccata gaaaaggtgc aatacccttt ttgcgaaagg
    10321 cgttaactta tttgcgtagt ataagctaat gatacgtttg ttcacccggc tagcggtagg
    10381 atagggatag accttgccga agatatgctt taacttaagt ccgctcgagt ttgcaagaat
    10441 gagttcttga gaaagttccc ctgcgttctc cgccaccata gagccaccga ggattttatc
    10501 ttttgcaatg aatagcagaa gctttccatc atgggactca tcaacaattg ctcggtcgtc
    10561 ctcttcaaag tcaaagacaa ccttttcata tgaaattcca cgctgcttga gttgctcctc
    10621 gttcaagcca aacgtggcga cctcaggtga ggtatatgtc acccaagcca tgtggtcatt
    10681 gttcagcttt ttcttaaagg gggagaagaa attgtttatg atgacacctg catgaacctc
    10741 agcggcatgg gtgaactggt aggagccagc aacatcacct gaaacaaaga cacgcttatt
    10801 tgtagtacgc aggtattcat caacctttag cttttttcca ttattctcaa gctcaatgcc
    10861 agccttctca agagataaac catttatatt ataatccctt ccaatgctca cgagcactgt
    10921 gtcgaagtca agccttatct ccttcttatc cttgttgaca atgattgcct gattagatga
    10981 gggaaactcc ttaacagttg cgttgaagat gaaatcaacg ccctccgata ccattcgccg
    11041 gtgtaggaca tctgcaatct cggggctctc ttttggtaga aattttgaat ccatttctac
    11101 aaccgtaact tttgaaccga gatgccggaa agcctgacct agctcaatac caattggccc
    11161 gccgccaata acaagtaggt gctcaggaag tttctcaagg tcgaatattg tttcattagt
    11221 ttcatatctg acatttccaa ttccgggcac attgagcttt ctaggtcgag agccagtcgc
    11281 taggattatt ttcttgccac gatatgtctc gtttcctaca gtaactaaat caggtccggt
    11341 gagcctggct tctcctaggg cgacgtccat tccaatcttg cggaagtgct cggcattttc
    11401 atgaaccctg ataacctctt gcttttcctt cacatagcct aaaacctttt tgatgtcggt
    11461 tttgccattt accgtaagac caaatctact tgcctctttt gcattcttta gaagacgtgc
    11521 aacatggatc aatgccttac tcggcacaca accagagttt agacagtcac caccaatacg
    11581 cctgtcgctc ttgtcaataa gaagcacctt aaaaccagca cgattcataa agcctgctat
    11641 gttaagtcca ccacttcctg cgccaatgac aataatgtcg tattttctca ttgtgaatta
    11701 ccctctttat cgaattttat gaagtaaaaa taatataatc taatttgtag cttagtatta
    11761 gacggttatg ggtctgacaa agtttcatgg aattgcttca aaaaacttca aaaattacag
    11821 ttagttgaga taaactaatg tcaaactttt tctatacccc ttgacaatag ttaacctata
    11881 gttatattat tatagtaatt aactagagag gttgactaaa atgattggag aaaaaattaa
    11941 aaacctgaga gaatctgagg gattaaccca ggaagaggtt gcggtaaaag ttggagtatc
    12001 aaaggagttt atctcgatgg ttgagtccgg caaaagaaat cccagcctcg aagtactatc
    12061 gaaaattgct tcagttttca gtaaagaagt aagccatttc ctgcagactc caaaagaaga
    12121 cttcgcttta gctctaagaa cggcagaaat aaacgagaag gacaaagatg aaatcataaa
    12181 gtacaaggaa ttctgtgagg attattactc cttagaagag gttacaggag aaatcctcca
    12241 acctcttgct ccgatttatc ccgacccatc ttctagtatc ttaagcgact tcggtcagac
    12301 ataccgctat gctgagaagt tagccctgga tgaaagaaga aggttgagtt tgggggatga
    12361 gcctataaaa gacattttcc tcctagtaga aactcaaggt gttcgtgttg taaggataaa
    12421 tatggtggat tcatctgttg acggtgcctt tatttacagt agggataaag gggcttttat
    12481 gcttataaac tcctttctaa gtaaaggtag acaggttttt accgcagccc acgagtactg
    12541 ccattacctg aaggacagag aaaaaggcta tccggtttgt cagattataa cgggtgacat
    12601 tgaagagcat aaaaaaccaa tagagcgcat tgccaattta tttgccgcaa actttctcat
    12661 gcctgaaact acagtctcaa agttagtagg tctgtatgca aaaaacaggc ttgaagctga
    12721 agaggttgtt tatatcaaaa gatattttgg cgtgagctac caagcaatgc tctataggct
    12781 aaaggattta agattcatta gaagacccaa gcttcaggaa gtactaaaga cagaccccgc
    12841 gacattagag atagctcttt ttggatttac agaagagagc gtgaaagacc ctgagcgtct
    12901 gcctgagaga tactgcaagc ttgcagtcca agcttataca gagaaaaaga tttctctcga
    12961 aaaattagca gaacttttaa aagtagattc ggttgaactt agagaaagat tatcaaaggg
    13021 aggttttatt aagtaataga gggaactgag agagaaaact atgtgcctct taggtgagtt
    13081 gatcgtgaaa ggttctaccg atttcacacc ttggcacgct ttttgaacct taaatgtatc
    13141 tacttaattt cattgaaaaa aggaggtttt agttatggag catcagatta aagagtttac
    13201 atccccagtt ttagataagc ctctcaggaa atataagatt cattgtccgc ttcatttctt
    13261 tgcctttgga acatttaaaa aagttttact aaaaaatgga tatgaagtcg ctcatcctga
    13321 aggaatattt taccatcgcc ctaatagagc acttatctat ccgacaatag caggaccaca
    13381 cgagtactac acactaatgt ttttaaccac ccttgatttc aaggacttag agaacctatt
    13441 ctttcaggca tttaatgaag attataaaaa taattatgta ctcatagagg gaatagaatc
    13501 ctgcagagtt cgaaacagtt taagcggtcc acactttgat gaaataaaac acactttacc
    13561 acaatcagtt gtaacaattc tggaaaactc cctatggaaa tttgaagatt tttgtaacag
    13621 gttttctaga tataacatgt ccaacactct gggaatgtta ttttatggtc aacccggtgt
    13681 cggaaagacc tatatactta ggagctattt caataagctt cttcttgaaa ggaactttac
    13741 tatagtccag gtttatcagg actgccttga ttacatgaat atgtcggtac ttttaaacag
    13801 ttgtcaggct ttgtttccat gcatcctatt tttagaagac atagacgtga atttcaaaga
    13861 taggagagaa ccagctaata gtttagttgg atttttactt gaaacttttg aaggactatt
    13921 aaatgtggaa aaggtcgtgc ttattgctac atcaaataat gtagatatga tagaaaaagc
    13981 cctgcttcgt cctgggagaa ttgactatct tgtagagctt gaaaagccct ctaagagtgc
    14041 aaaagaattg gtcttatcca attatctaaa tggatcggat ttaaatctac cagatcctct
    14101 aaaagaacta ctgataaata acgttgatac ctttgccgaa ctcaaagggg gattccagca
    14161 tttgctaaga acttacgtaa acaaaggaga ttttccatct atggaggaag tttcaaggtt
    14221 gataaggacc tggaaggaag caaaagttgt gggtggttta gaaaatggag aaaggaggat
    14281 ggggttagtg tgagggaatt aatctctaag aaataaaatg attagtaata tgaaactaag
    14341 aaaaaagtta tgtgtgtcca aaggaggaat caaacatgaa gaccgacctt tatacaaaaa
    14401 tagttcttac cataatcgcg atagctctat gggggctttt gcttaaaccg acttttgttt
    14461 ctcagagcgt tggtgcatca actcaggttt tggatgtaaa tataaaagag ataggtggac
    14521 gagcgatcaa cgataaaatc cttgatgtaa atatagagaa aataaatgga cgcaccttta
    14581 ctacaactat tccccctgct cttcctgtag agataaagaa ataggcttaa tttatggctg
    14641 ctctactatc ggtagatgag agtaaagtgg aggcaggaga tcaagaagat tgtgttcttt
    14701 ctcattaaaa aggtctatca attttaacgt ttaaggcagg gtaaaaatat gtcaaaaaat
    14761 ctatcaaaat caaaatacct ctcgggtctt cagtgcgaaa agcgtctatg gcttgagatc
    14821 aacgatccag ataaagcttc ggagacaccg gaatatcagc aaagactttt taatcagggt
    14881 aaggaagttg gaattcgtgc tcggagatat ttcgaggagg gttatttaat tgataaggat
    14941 cgtttaaaaa tttatgaatg cgtcgaggaa acccgtgatg caattgctag aggagagtca
    15001 ataatctttg aag
//
LOCUS       DATDRL010000126         6026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_939548, whole genome shotgun sequence.
ACCESSION   DATDRL010000126 DATDRL010000000
VERSION     DATDRL010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939548"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1455)
                     /locus_tag="VI935_04540"
     CDS             complement(49..1455)
                     /locus_tag="VI935_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HLE24902.1"
                     /translation="MRKDRFVLGWEVDSDETLFMKSVLSNRVKAVMESHHGMWNYMTY
                     RDAIKILGLDTEEIYKEGRVDLDPKKWADFGWMTCYVGPPKSAVKAMREQTKASNINP
                     YSPDLINPLRDDCAEIKLKRERSKEFEVIGTEGGQAGISYALQTFINPGDEVIITDPG
                     YFHFESAILMVRGVPVRIPLNSRNGYRLNPDEVKEHITPRTKVIIVCDPLNPFGTVQL
                     KDELIALIEITRRHDIIIIDDITHNSQRIDPNAIHYPMSSLWREINVDNVVSTFSVSH
                     GYGMAGIRIGFLAGHTELMRACLIAKVSLTRLNTNLIAQYGALAALREENYIRKSEYI
                     IRRNYNHIKKTISQTNGVSIPVEPKYGFSMVVDVSGTGVTAQELTVALFKHRVAVYPG
                     DGLGNVGATDYIRLNISRPDLWAFRHFRKVLPLAIKEARSGIYRKVVIQFFKEKKTER
                     AKRILQQMKDMHLADEQY"
     gene            complement(1670..2518)
                     /locus_tag="VI935_04545"
     CDS             complement(1670..2518)
                     /locus_tag="VI935_04545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HLE24903.1"
                     /translation="MHLSRMPSFEELLKPLPIYSPKNQYYKCYSIFLRTIGIFSEGMK
                     IGFTHGFDSGMIMNYVYQNTPSGRFYIGKVLDRIFLNQVTCKAFRAIKEIQKNVIKNY
                     LQGRNGNPTFIVDLASGKADYIYDALRETNSNVKVLLRDIDETALKESRAIAQKLNLL
                     NKVSYEKGNALDVKSLERIHPKPNLIVEVGLYGIIHDDELLRRHFFELKNIINPGAIL
                     FNVQTYNPQIELIARVLKNRDGERCVWHLRPVELVIGWAEKAGFIEPQVTMDPYGIYS
                     VVMMKG"
     gene            complement(2508..3074)
                     /locus_tag="VI935_04550"
     CDS             complement(2508..3074)
                     /locus_tag="VI935_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24904.1"
                     /translation="MSIRNPERLKEVLSNPKRFARAFSRHTYGMLAWVDLFGGKLKTI
                     RDLEMKLVAARIIADNAKHAKLFSDRAKELGEKPETYKPPLIGQKIYDFLDAYDDPFD
                     DFAYAWGSLIHFSSLLDLYNSVADSKSRVVVEEVQRDVKEHLKLLDKYFELNANTPEK
                     KKRSEEIKMLADRIYTDREDEEIKWYAS"
     gene            complement(3169..3624)
                     /locus_tag="VI935_04555"
     CDS             complement(3169..3624)
                     /locus_tag="VI935_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24905.1"
                     /translation="MVTKFKIKKNYFQDALRLMRISKSIREIEGVKKAVAVMATEKAK
                     FALEEAGLMMPEIKDASGSDLIMTVEAEAEEVANQVLVKMEELVSAGASQSKKESSDI
                     LHQEIQAINIGLEVFKEALESQGVKVVQVNWQVPAKGDMKLINILKKMY"
     gene            complement(3774..4166)
                     /locus_tag="VI935_04560"
     CDS             complement(3774..4166)
                     /locus_tag="VI935_04560"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HLE24906.1"
                     /translation="MEVQAVWKKNYQVEVKARQFQVSIDEAPEFYGEDTGMMPTELFL
                     CSLASCFCLALVFVAGKKRIEVKDMMVNVKGKKDLKNFIFSSLLVEVESSLAPDTLEE
                     IIPLAKRYCFVSNTVSKSCPIEYAVKNV"
     gene            5415..5756
                     /locus_tag="VI935_04565"
     CDS             5415..5756
                     /locus_tag="VI935_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24907.1"
                     /translation="MAFAEEIKKYDVAYYAGGKNTTGYEYRAIIGLRREDGSLIGGAY
                     FHRDPATMPNTDEQTSLGYVWCHYTWEDFPQVLDLLRNEKPVYVRYVAGTWNIASITT
                     SLEPVGEGEQP"
     gene            5778..>6026
                     /locus_tag="VI935_04570"
     CDS             5778..>6026
                     /locus_tag="VI935_04570"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLE24908.1"
                     /translation="MFNSVLTRTLVWFYAASEAELLAGSPRGGRTYMKELHTDIEIAA
                     PVERVWALLVDLQFYKDWNPFIREGHGELREGARIRVRI"
BASE COUNT         1673 a         1380 c         1131 g         1842 t
ORIGIN      
        1 aagataggtg aaccgtactc gtgaactatc ttatcagtag aaatgccatc aatattgttc
       61 atcagccaag tgcatatctt tcatctgctg gagaattctt tttgcccgtt ccgttttctt
      121 ttctttaaag aactggatga caaccttacg ataaattcca gatcttgctt ctttgattgc
      181 tagtgggagt acctttcgaa aatgcctaaa agcccagagg tcaggacgag agatatttaa
      241 ccgaatgtag tcggttgctc caacattacc caatccatcc ccaggatata cagccacccg
      301 gtgtttaaag agggccactg tcagctcctg agccgttaca cctgtgccag aaacatcaac
      361 aaccatagaa aagccatact taggctcaac aggaatagat acaccatttg tttgactaat
      421 tgtttttttt atatggttat agttgcgcct tattatatat tctgatttcc tgatataatt
      481 ctcctctctt agcgcggcta aggctccata ctgagcaata aggttagtgt taagccttgt
      541 caaagaaacc ttcgctatta aacaagccct cataagctcg gtatgacctg caagaaagcc
      601 gatgcgaatc ccagccatcc catacccatg agagacactg aaggtagaaa ccacattatc
      661 tacgtttatt tccctccaga gagaactcat agggtaatga atagcattcg ggtcaattcg
      721 ctgggagttg tgtgtaatat cgtcaattat gataatgtca tgtcttcttg taatctcgat
      781 gagggctatt aactcgtcct ttaattgcac agtgccgaaa ggattaagag ggtcacaaac
      841 gattatcacc tttgttctcg gcgtaatatg ctcttttact tcatcaggat ttaagcggta
      901 tccattcctg gaattgagag gaatcctcac gggcactcct cttaccatta gaattgcaga
      961 ttcaaagtgg aaatatcctg ggtcagtaat aattacctca tctccaggat ttataaatgt
     1021 ctgaagcgca taagaaatcc cagcctgtcc cccttctgtt cctataacct caaattcctt
     1081 gctcctttca cgctttaact ttatttcagc acagtcgtct ctaaggggat ttataaggtc
     1141 tggagaataa ggattgatat tagatgcctt tgtctgctcc ctcattgctt ttacagcact
     1201 cttaggaggc cccacatagc atgtcatcca tccgaagtca gcccattttt tcgggtctaa
     1261 atcaactctt ccctctttat aaatctcttc agtatccaac cccaagattt ttattgcatc
     1321 acggtatgtc atatagttcc acattccatg atggctttcc atgactgctt taaccctatt
     1381 tgataaaaca cttttcataa acagtgtttc atcactatca acttcccatc cgagaacaaa
     1441 cctatccttt ctcaatttaa tatctccttt gccggtctcc ccaaacatta gccactaatg
     1501 aacacgaatt tacacaaata atcaatcctt ggaaaactaa agctttccgc tacctacaaa
     1561 agttttatga aaaagaaata tataactgaa gtggaatcct ataggattcc atcaaaatat
     1621 aaatttttaa ttgttttttc attcgtgcct attcatggtt aaataattac tagcctttca
     1681 tcatcacaac tgaataaata ccataagggt ccatagttac ttgtggctca ataaatcctg
     1741 ctttctcagc ccaccctata acaagttcca cgggacggag atgccacaca caacgttccc
     1801 catcacggtt tttaagcacc cttgcgatta actctatctg cggattataa gtctgaacgt
     1861 taaaaagaat cgcgcctggg ttaattatgt ttttaagctc aaaaaaatgc ctgcgtaata
     1921 actcatcatc atgaataatc ccgtataggc ccacttcgac aatgaggttc ggcttaggat
     1981 gaattctttc caaactctta acatcaagtg cattaccctt ctcatagcta actttattta
     2041 gtaaattcaa cttttgagct atagccctgc tttctttcag cgcagtctca tcaatatcgc
     2101 gaaggagtac ctttacattg gaattagttt ctctgagagc atcgtaaatg tagtcagctt
     2161 tgcccgaagc caagtcaacg ataaaggtgg gattaccatt tctcccttgt agataattct
     2221 taattacatt cttttgaatt tcttttattg ccctgaacgc cttacatgta acttgattaa
     2281 gaaaaattct atccagaacc tttccaatat agaaccttcc actaggggta ttctggtaga
     2341 catagttcat aatcatccct gagtcgaagc catgtgtgaa gcctattttc attccttccg
     2401 agaatatacc gattgttctc aggaaaattg aataacattt atagtattga ttttttggag
     2461 aatatatagg aagtggcttt agcaactcct caaaactagg cattctacta agatgcatac
     2521 cattttatct cctcgtcttc cctatcagta taaatcctgt cagctaacat ttttatttct
     2581 tcagagcgtt tttttttctc cggtgtattt gcatttagtt caaaatattt gtctaaaagc
     2641 tttagatgct ccttaacatc cctttgtact tcctcaacta ctaccctact ctttgaatcc
     2701 gccacgctat tatataaatc aagcaacgat gagaaatgta taagagagcc ccatgcataa
     2761 gcaaaatcat caaaggggtc atcgtacgcg tcaagaaaat catagatctt ctgtccaata
     2821 agtggtggtt tataggtttc tggtttttca cctaattcct ttgccctatc tgaaaaaagt
     2881 tttgcatgct tagcgttatc agctattatc ctagcagcaa cgagtttcat ttcaaggtct
     2941 ctaatggttt ttaacttccc tccaaataaa tccacccatg caagcatacc ataggtgtgc
     3001 ctgctaaagg ctcttgcaaa ccgcttggga ttagaaagaa cctcttttaa tctctcagga
     3061 tttcgtatgc tcattatgct ttcgttataa ccacaacgac actaagacac aaagatttat
     3121 tttaatattt ccttttcgtg gtttcgttgt gtcgtagtgg tttcaaaatc aatacatctt
     3181 ctttagaata tttatcaact tcatgtcacc tttggctggg acttgccagt tgacttgaac
     3241 tacttttact ccttgtgatt cgagcgcttc cttaaaaact tcaagcccaa tatttattgc
     3301 ttggatttct tgatggagta tatctgaaga ttctttcttg ctttgagatg cgccagccga
     3361 aactagttcc tccatcttaa ctagtacctg attagctacc tcttctgcct cagcctcaac
     3421 ggtcattatt agatcactcc cgcttgcgtc ttttatctca ggcatcataa gccctgcttc
     3481 ttcgagtgca aactttgcct tttccgttgc catgaccgca acagcttttt tcaccccttc
     3541 aatctcccga atgctttttg atatgcgcat cagacgcaga gcatcctgaa aataattttt
     3601 cttaatcttg aattttgtta ccaaaccatt tgcctccctc aacaatagta ataagctaag
     3661 ctcagagatg tcattgcgag cgaatgaaaa tgagcgaagc aatcccctta gattgcttcg
     3721 tcgctatcgc tcctcgcaat gacctctttc tccttaaatg gcattgttct cctttagaca
     3781 ttctttaccg catattctat tggacacgac tttgaaactg tattacttac aaagcagtat
     3841 cttttcgcta gaggaataat ctcctccaaa gtatcaggtg caagagaact ttcaacctca
     3901 accagaaggc tcgaaaagat aaaatttttg aggtcttttt tccccttaac atttaccatc
     3961 atgtccttaa cttcgattct tttctttccg gctacaaaaa caagtgcgag gcaaaaacaa
     4021 gaagcaagag agcagagaaa aagctccgtt ggcatcatac cagtatcctc accataaaac
     4081 tctggggctt catcaatcga aacttgaaac tgccttgcct taacttcaac ctgataattc
     4141 tttttccaga cagcctgaac ttccatgatt gcacctaaaa tattttttta actcacgtta
     4201 cgtatatttt acgcattgtc atgcccgaat gtatcaatca ggcatcctaa aagtatttaa
     4261 atagtagatc ccccgcttaa acttgtaccc acatggttta agtggttagc gtgcgggaat
     4321 gacagcttta cataccccat gagtgtatga attcttagca tggatcattt aatattaact
     4381 caaaaaccat gcgttgagga tagaagtgat cagaatatac ctctctataa gacacccctc
     4441 ttaacatagc cctcgccagt acggctagaa tattttagtt atatagcgcc aaagacttgg
     4501 tcgaagagct ttttgggccg cctttctgca ttcaagggaa taatgacgct tgagattgta
     4561 ctccatttaa cctagcatgt aatcgccttt gtaattgtac agccttcatg ctatataata
     4621 cacccgtaaa gctgattaga cagcaaaatc gttagtcgaa taaatgatgt ctaataaata
     4681 gttgggctgt gttaagatta tgtagcgatg gctatgttga aatggctcaa gtcacttttc
     4741 tccatgccag aaccggcagg acccccgcag acgttacgag tcttcacggt cgacaaacct
     4801 actatcacgc gaggctgcat acacgccgag aatgatcggt ggcgtatcga ctccaccgaa
     4861 gaacaaactg tacgactttt tgaggttgaa aaccccgaat tagaacagtg catgcttacc
     4921 tatcggatgg aaattagaac agaaggcctg caaggccggg ccttccttga gatgtggtgc
     4981 cgcttgcccg gtcggggcga gttcttctcc aaaggctttc aacaagcggt ttctggtact
     5041 actgactggg cgcgatacga gatcccgttt tacctgaaaa agggccaaag gcccgacctc
     5101 attaagctta acctcgctgt ggaggggacg ggtacggttt ggctcagaaa cgtcgagcta
     5161 ctaaagaccc cgctagcatc gtgagatgta gcatgcttaa cttggtgaac ttagctttcc
     5221 ccagtactgt tacgatagct tttgagctta tcgcctcgtg gatttgctcg aggaatatcc
     5281 ttggtcgcct ttctgtatta ttttaggttt ttcagataaa atcccgtata gggtgattat
     5341 atgagctttt gttcttataa gtatatagtg agacggcagt taaatatgaa cgcaaagaaa
     5401 ggaggcttgg cgcgatggct tttgccgagg aaatcaagaa atacgacgtt gcgtattacg
     5461 ctggtggaaa aaatacgacc ggctacgagt atcgagcgat tattggcttg agaagggaag
     5521 acggcagcct aatcggtggc gcatacttcc atcgagatcc tgcgacgatg ccgaacaccg
     5581 atgagcagac ttcgttaggc tatgtatggt gccactatac atgggaggac ttcccgcaag
     5641 tcttagatct actccgcaat gaaaaaccgg tttatgtccg atatgttgct ggcacttgga
     5701 acatcgcttc catcacgaca tcgcttgagc ctgttggcga gggtgaacaa ccatagaacg
     5761 gctgcctagc tggctcattg tttaacagtg tgcttacacg aacgctggtt tggttctatg
     5821 cggcgtctga agctgagctg ttagccggat cacctagagg gggtagaacc tatatgaagg
     5881 agttacatac agatattgag atcgctgcac cagtcgagcg ggtttgggca ctgctagtgg
     5941 atcttcagtt ctacaaggac tggaatccat tcatccgcga gggtcacggt gagcttcgcg
     6001 agggcgcccg aatacgcgtt cgaatc
//
LOCUS       DATDRL010000127         8013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3767306, whole genome shotgun sequence.
ACCESSION   DATDRL010000127 DATDRL010000000
VERSION     DATDRL010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3767306"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VI935_04575"
     CDS             complement(<1..335)
                     /locus_tag="VI935_04575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HLE24909.1"
                     /translation="MQKMTKSEYRNFLLEGTRTGKLAMARNDGRPNVVPIWVDLDGDT
                     IVFTTGGSSIKAFNMRRDPRVCVCVDDQIPPFAYVQIEGTAALTDNPDELLYWATRIG
                     GRYMGAELA"
     gene            758..1105
                     /locus_tag="VI935_04580"
     CDS             758..1105
                     /locus_tag="VI935_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24910.1"
                     /translation="MLSDRRIDFSDTKEKEWNELRSKIADGIELPLPDSERWFLATLE
                     GDKIRIDGARKNVRALKVYNPVTITFKEFAVVAEIYNDCLFGGLDVLSPKLELQKTMV
                     NFRYIFILIYNLL"
     gene            complement(1368..1622)
                     /locus_tag="VI935_04585"
     CDS             complement(1368..1622)
                     /locus_tag="VI935_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24911.1"
                     /translation="MNRVYYRRILAVLIICALIVASFISGSVIADQPHMHSALDHLRA
                     AREQLEQASPDKGGHRVKAINLVNEAIREVKAGIEAGRYY"
     gene            1774..2631
                     /gene="mepA"
                     /locus_tag="VI935_04590"
     CDS             1774..2631
                     /gene="mepA"
                     /locus_tag="VI935_04590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF006947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HLE24912.1"
                     /translation="MAKNAKPTIKPQSLPILTLFVIFCLYAENQLKADEPNKSEVIGF
                     YTSGCIANSLPLPEAGTGYQVIRLSRKRFYGHPNLIEYVKSLGQSVASYRTGTLLIGD
                     LSQEKGGPLPDDHNSHQNGLDADILFWQNPVAKTRILSISERENIYPLSLLTTTNNYP
                     RIDYSKWNSIHGGILKIAASSGKVDRIFVNPLIKRNLCTIYEGEDWLSKIRPWWGHDG
                     HFHVRLSCPDNSPLCKPQKPVPEGDGCDTDLGSWLRKIARIQKKDDKPRPKARTALPK
                     ECLTFLSEY"
     gene            complement(2725..2976)
                     /locus_tag="VI935_04595"
     CDS             complement(2725..2976)
                     /locus_tag="VI935_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015109732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24913.1"
                     /translation="MSKISSRTIQTALLIGALFVVAFIAGRVTADQPHMRAALASLQV
                     AQSELQQAIPDKGGHRVRALELVNQAIVEVQAGIEFAEY"
     gene            complement(3110..4333)
                     /locus_tag="VI935_04600"
     CDS             complement(3110..4333)
                     /locus_tag="VI935_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HLE24914.1"
                     /translation="MTLRSLYVDFDSYFASVEQQLRPELRGKPIGVVPVMADTTCCIA
                     ASYEAKRFGVKTGTRVSDARKLCRGMQFVEARPAVYVEYHHKLVEAVESCIHVDSVLS
                     IDEMLCELTGSMKQYERATAIGRKIKETIANRVGTELRCSIGIAPNPFLAKTASEMRK
                     PNGFVVIDLQDLPNCLFSLNLRDLCGIGEAMERRLREHGINTVEDLCNASKYKLRKAW
                     GGIEGERMYARLRGELVYTPPTRRATVGHSHVLPPELRSEEASFSVLNKLLQKAAMRL
                     RSYGLVAGALLIRLKYIDGRSWDTKIGFDPTGDTLEFLHALSRLWAERPKTKAVPLAI
                     AVTLFNLSEEKSCSLSLFKNDRSRERLNALIDKLNLRYGKNTVYFGGAQIGLNAAPMR
                     ISFTHIPDITIESDE"
     gene            4534..4824
                     /locus_tag="VI935_04605"
     CDS             4534..4824
                     /locus_tag="VI935_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24915.1"
                     /translation="MNTRKQNEFTGTLPISARPLVEAVNVDWSIPIYVIHSRQDEVFR
                     IEDAVKAVELLSKRKASVELKIVEGVTHYGTYGFIEPLKEAISWIRRVWGQF"
     gene            complement(4856..6190)
                     /locus_tag="VI935_04610"
     CDS             complement(4856..6190)
                     /locus_tag="VI935_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HLE24916.1"
                     /translation="MASRQPIIFILLLIIIVITLSKAHAGDQKPKELTIIWHSGDLSE
                     YLIEISKQYTKQTGVKINVELVPWDKWHDTIASEFDSKGSRFDLVVFDSQSMSEFASE
                     GNFVLLNPYIEKSKKVKFTDYDPEALREYAEYPEGSGKLYALPLNQDAMGLVYRKDLL
                     EDPEEKKAFKVKYGYELTVPETYEQLKDIAEFFNRPVKNLYGIALYGSRDYDAVTTAF
                     NNVLWSFGGELWDPRTRRAEGFINSSASVAALEYFKELFKYAPPGATGWYYDEVNNAI
                     NNGRVAMGINWYYFFNANSNPKISKYADKLGFAPLPGEKGPDGKFRQYNSLGGQGISI
                     SKFSKHVDEAWRFLEWFVSNENQWEWVKGGGQTGRVDILRSPSYANATPYNSVFPVSM
                     SRVKDYWHLVEYPQLLDIYQKYVNLAITGAMSPKEALDNVAKEQQAVLDKAS"
     gene            complement(6280..6564)
                     /locus_tag="VI935_04615"
     CDS             complement(6280..6564)
                     /locus_tag="VI935_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HLE24917.1"
                     /translation="MPRNIIERDIPEIGSVEREALREAAHKSNSVLSEMRAEKKNIQC
                     EHSYVASDKIFCIYITDNEALIQEHAKRSGFPATKVTELRKIIGPVTAEG"
     gene            6769..7455
                     /locus_tag="VI935_04620"
     CDS             6769..7455
                     /locus_tag="VI935_04620"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HLE24918.1"
                     /translation="MIVTTHQPIFLPWPGLFFKAMKADCIVLLDDVQFPQGRGWVNRN
                     RLKNEQGELWLTVPVWKKGRGLQIIRKVEICNETDWQRKHLQSIRQSYANAPYLKGYF
                     PILESIYRENNRLLIDLNLNIIKFFWDSLDLKTELYLQSELGVTGKGTELIINICKHL
                     QADTYMTFPIVEKYLDIFQMNQSGIQIKFSSFNPPVYPQLWGEFIYNLSTLDMLLNCG
                     EKSRDIIVSE"
BASE COUNT         2381 a         1763 c         1561 g         2308 t
ORIGIN      
        1 tcagctaatt cagcacccat atatctgcct ccgatccgag tagcccagta aagcagctca
       61 tcaggattgt ctgtcaaggc tgctgtacct tcaatttgta cataggcaaa aggtggtatc
      121 tgatcatcaa cgcaaacaca cacccgcggg tcacgccgca tgttaaatgc ctttatcgaa
      181 ctgccaccag ttgtaaaaac tatagtatca ccatcgagat caacccatat tggaactaca
      241 ttagggcgac catcatttcg tgccattgct aacttgcctg tccgggtacc ttcgagcaaa
      301 aagtttctgt attcagactt agtcattttc tgcattgcat tgccccctct taaaaaaccg
      361 atttacatct tgctcattat aacttaatga ttttgatgtt gacaataaat gataaaatca
      421 taagtaatca cactttatca aggaatggta aaaggcagta agctccaatt ctatcaaata
      481 tttgtccata ccaattgggt cgtttttcca ctaacagcaa tgatgtgtag tagccataag
      541 gtaggggctg ataccctcat cagtctcccg atgaagttgt cgggggctcg ctcggggacc
      601 gagccctaca tttctcttta ttgtaatggt ggttgttgtt atgcactatt aacaaatcga
      661 accggtataa gctgtaagat tttcactata aatctatttt ataatgtgtg ttatatttgt
      721 atgctctgtt ctgtttgctt aaaggaggta gttattaatg ttatcagaca gaagaattga
      781 ttttagtgac acaaaggaaa aggagtggaa tgaactaagg tcgaaaatcg ctgatggaat
      841 cgagttgcct ttacctgatt ctgaacgctg gtttcttgca actttagaag gagataagat
      901 taggatagat ggagccagaa aaaatgttcg cgctctaaag gtttacaatc cagtaactat
      961 taccttcaaa gaattcgccg tcgttgcgga gatttacaat gactgccttt ttggtggact
     1021 cgatgtacta agcccaaaat tagaactaca aaaaaccatg gttaacttcc gctatatatt
     1081 tatactgatt tataatttgc tttagaaaca ttaataagac atatatataa atcgtctttt
     1141 agataatttt tatcttttgt aaaaccagcc agtgttagat cataatctct ttatgtcttt
     1201 catctcttat aattaaacaa aaggccagca tttattacca gtttgaacaa ttagatccct
     1261 ttagtaagga taggaacgca aatgagacag caccttgttg aaaggtggaa ctactatttt
     1321 gatgctatgt ataagagaaa agatcgcaga agtgtatctt ttaacaatta gtaatatcta
     1381 ccagcttcga tcccagcttt tacttcacga attgcttcat tcaccagatt gatagcctta
     1441 acacggtggc cgcccttatc gggacttgct tgctcgagtt gttccctagc cgccctaaga
     1501 tggtctagtg ccgaatgcat atgaggctgg tcagctataa cactaccaga gatgaagcta
     1561 gcgacaataa gcgcgcatat aataagaaca gctaaaattc tgcgatagta aactctattc
     1621 ataataagac ccccctttat accatcattg tctgacaatt cgttataact cttaaaaacg
     1681 gatcttgttt catcctgaaa gttttcccaa aaagataatt atttcaaatg ccatattgaa
     1741 attgctttct cttaggcata caataaacac aaaatggcaa aaaatgccaa gccaacaatt
     1801 aaacctcaat cgttaccaat cttaacctta tttgtaatat tttgtcttta tgcggagaac
     1861 cagttaaaag cagatgagcc caacaaatct gaggttatag gtttttacac atcaggctgc
     1921 atcgcaaact cactcccact gcccgaggct ggcacaggct atcaggtgat tcggttaagc
     1981 agaaaacgat tttacggaca ccctaacctt attgagtacg taaaatctct aggtcaatca
     2041 gttgcttctt atcgaaccgg cacattactc attggcgatt tatcgcagga gaagggtggg
     2101 ccattgcccg atgaccataa tagtcatcag aatggtctcg acgcagacat tttgttctgg
     2161 caaaaccccg ttgctaaaac aagaatactt tccatatctg agagagagaa catctatcct
     2221 ctttcgttac ttacaaccac gaataactat cctagaattg attattccaa atggaattcc
     2281 atacacggcg gaatattaaa aatcgccgca tcttcgggaa aagtggatag aatttttgtg
     2341 aacccgctta taaaaagaaa tctctgcaca atatatgaag gcgaggattg gcttagtaaa
     2401 atacgtccat ggtgggggca tgacggacat tttcacgttc gcttaagctg ccctgacaat
     2461 agccctttat gtaaacctca aaaaccagtt ccagaaggag atggttgtga tactgatttg
     2521 ggtagctggc ttagaaaaat agcccgtatt cagaaaaagg atgataagcc cagaccaaaa
     2581 gcaagaacgg cgttgccaaa ggagtgctta acctttttgt cggagtatta gtattttttg
     2641 cagcgacggg aatttttaga gacaatcttc tctatatgct gttcatcttc aatcattacc
     2701 tctaaacgta caactatcta ttttctaata ttctgcgaat tctataccag cctggacctc
     2761 aactattgct tggtttacaa gctcaagggc cctgacacga tgcccacctt tgtcagggat
     2821 ggcttgttgt agctctgatt gtgccacctg taaagaagcc aaggcagcac gcatatgtgg
     2881 ttgatccgct gtaactctgc cagcgataaa agcaactaca aaaagcgcgc ctataagaag
     2941 cgctgtttga attgtacgag aagaaatctt actcatttta agatcctcct tatattgaag
     3001 cttttaaaat tccctgtggt caaatttcga aaaccgttag tattatattc caatagggag
     3061 tatagtcaaa aggacaagtt agtaaaatac cctataattg cttgctaaat tattcatcgc
     3121 tttcgatcgt aatatcaggg atgtgggtaa aagaaattcg catcggtgcg gcgttcaggc
     3181 cgatttgcgc cccgccaaag taaacggtgt tcttaccata acgcaggttc agcttgtcaa
     3241 ttagagcatt aagccgctca cgcgacctat catttttgaa tagcgagagt gaacaactct
     3301 tttcttcgct taagttaaac agcgtaaccg caattgctaa tggaacggct tttgttttag
     3361 gccgctctgc ccataatctt gaaagcgcat gcaggaattc aagtgtatcg cctgtgggat
     3421 cgaaaccgat tttagtgtcc cagcttcgac cgtcaatgta ttttagtctt atcaataagg
     3481 cgcctgctac caagccatag cttcgcagtc tcatagcggc tttctgaagg agtttattca
     3541 gaaccgagaa ggaggcttct tcagaacgca actcaggcgg taatacatga gagtgcccaa
     3601 ccgtcgccct cctggttggc ggcgtgtata ctagctcgcc gcggagtcta gcgtacatcc
     3661 gctcaccctc aatacctccc caggctttgc gaagcttgta tttactagca ttgcagagat
     3721 cctcgactgt attaatgccg tgctctcgga ggcgcctttc catagcctca ccaatcccac
     3781 ataggtctct caaattaagg gagaaaaggc aattgggtaa atcttgcaaa tcaatgacaa
     3841 caaaaccatt aggtttccgc atttcagacg cagtcttagc caggaatgga ttcggggcga
     3901 tgccgatcga gcaacgaagc tcggtgccta ccctgttagc gattgtttct ttaatcttac
     3961 ggccaatagc ggttgcacgc tcatactgct tcatactgcc ggtgagttca caaagcatct
     4021 catctatcga gagaacgcta tctacatgaa tgcatgattc tacggcctct acaagcttgt
     4081 ggtgatactc tacatagacg gctgggcgtg cctcaacgaa ctgcataccg cggcagagct
     4141 ttctggcatc cgacacacgc gtccccgttt taacaccaaa cctctttgcc tcgtagcttg
     4201 cagcaatgca gcatgtcgta tcagccatga ccggtacgac gcctattggc ttgccacgca
     4261 gctcaggcct caactgctgc tctactgagg caaaatagga atcaaaatcc acatagagtg
     4321 aacgtagagt cattggtcta aaaccagata agtcgcatca ccaaggcaac tctaacttat
     4381 catcacttca agattgtttc taacccgtaa acagtataat tatatcaaat ttccacgcat
     4441 aattaaccac caacgaattt cataagagat tctcgtgaaa ctccaagctg gagacaatat
     4501 aaaccgaata cccgcgaaac actaataagc aaggtgaata caaggaagca gaatgaattt
     4561 acaggaacat tacctatttc agcaagacct ttagtggaag ccgtcaatgt ggattggtca
     4621 ataccaattt atgttattca cagcaggcag gacgaagtat ttcggattga agatgcggtg
     4681 aaagctgtgg aactccttag taagagaaaa gcttccgtag agcttaagat tgttgaaggt
     4741 gtaactcact atggcacata cggatttatt gaaccactta aagaggcgat ctcatggata
     4801 agaagggtat ggggtcaatt ttgattcatt tagcttcgat gtcaaagact ccatgctaac
     4861 tagctttatc cagaacggcc tgttgctcct ttgcaacatt gtccaaagcc tccttcgggg
     4921 acatagcgcc agtgatagct aagttgacat acttctgata tatatcaagc agttgaggat
     4981 actccacaag gtgccagtaa tcctttacgc ggctcattga gacgggaaat accgaattat
     5041 aaggggttgc gttggcataa ctcggtgatc tcaaaatatc aacacgacct gtttggcctc
     5101 cacccttaac ccattcccat tgattctcat tacttacgaa ccattcaagg aatctccacg
     5161 cttcatcaac atgcttggaa aacttggaaa tgctgattcc ctgtcccccg agggagttgt
     5221 actggcggaa tttaccatcg ggacctttct ccccaggcaa aggggcaaaa cccaacttgt
     5281 cagcatactt gcttatcttc gggttgctat tggcgttaaa gaagtaatac cagtttatgc
     5341 ccatggcaac cctaccgtta tttatcgcat tattaacttc gtcatagtac caacctgttg
     5401 cgccaggagg agcgtactta aagagttcct taaagtattc caatgcagct acagaagcgg
     5461 acgaatttat aaacccttcg gctcttctag ttcgaggatc ccacaactca ccgccgaaag
     5521 accacaaaac attgttaaag gctgtagtga cggcgtcata gtccctacta ccatataaag
     5581 cgattccata gagatttttg actggtcgat taaagaattc cgctatgtct tttaattgct
     5641 cataggtctc tggaactgta agctcatacc catatttaac cttgaatgcc ttcttttcct
     5701 cagggtcttc taacaaatcc ttacggtaca caagacccat tgcatcctgg tttagaggca
     5761 atgcatacaa tttaccagac ccctctggat attctgcata ttcacgcaat gcctctgggt
     5821 cataatctgt aaactttacc tttttacttt tctcaatata ggggttcaat aaaacgaaat
     5881 taccctcaga ggcaaattcg ctcatggact gactatcaaa tacaaccaga tcaaatcgac
     5941 ttcccttaga gtcaaattca gaggcaatag tgtcatgcca tttatcccag ggaaccaatt
     6001 ctacgttgat tttgacacct gtttgtttag tgtattgctt gcttatttca atcagatatt
     6061 cgctcaaatc tccagagtgc cagattattg taagttcttt aggtttttga tcaccagcat
     6121 gtgctttaga aagagtgatc acaattataa tcaaaagaag gataaaaata attggttgcc
     6181 tgctagccat tctatttcct ccgacgttaa taagctattt atttgtcatt tcaaaacgag
     6241 aactcaatat tctaaatcca gaagacaact tgtagatcgt tagccttctg ccgttacggg
     6301 gccaataatt ttccgcaatt ctgtcacctt cgtagcggga aaaccgctgc gcttagcatg
     6361 ctcctgaatc agcgcctcat tgtctgtaat ataaatgcaa aatatcttat cgctagcaac
     6421 atagctgtgc tcacattgaa tgttcttttt ttccgcccgc atttctgaaa gcactgaatt
     6481 tgacttatgt gcggcctcac gcagggcttc gcgctcaact gaaccaattt caggaatgtc
     6541 tcgctcgata atgtttcttg gcattgtatt ccctccttaa gcttatatat gacggaaaat
     6601 aaagcctaac atcccctttc atagaactgt tgatcatacg gaattattcc ccataatcac
     6661 cccgatacaa ggtgttatac agaatcctgg tgccttttcc ctttcaagta tattcaaggc
     6721 gactctctat tcaatagtta tgagacaaat taaaattaag aacaaactat gattgtcacc
     6781 acacaccagc ctatattttt accctggccc ggattgttct ttaaggcaat gaaggctgat
     6841 tgcattgttt tactcgatga cgtgcagttt cctcaaggtc gtggctgggt taatcgaaac
     6901 cgtctaaaaa atgaacaggg agagttgtgg ttgactgtgc ctgtttggaa aaaggggcgc
     6961 gggctacaga taattcggaa agtagaaata tgcaatgaaa cagattggca aagaaaacac
     7021 ttgcaaagca ttcgtcaaag ttatgctaac gctccatacc tcaagggata ctttcccatc
     7081 ctggagtcca tttacagaga aaacaatcgt ctgttaattg atcttaatct caatattata
     7141 aagttctttt gggattcgct cgatctgaaa accgaactgt atctacagtc tgaacttgga
     7201 gtcacaggaa aaggcactga gttaatcatt aatatttgta aacatctaca ggccgatact
     7261 tatatgactt ttcccatcgt agaaaaatat ttagacatat tccaaatgaa tcagtcagga
     7321 attcaaatca agttttctag ctttaatcca cccgtctatc cgcagctctg gggagagttt
     7381 atatataatc tatctacgct ggacatgttg cttaactgcg gcgaaaagag tagggacatt
     7441 attgtgtcag agtgaaatgc actaaataac ctaagtttga tatagttttt taaacggata
     7501 accggatcgg gtaggcaacg agtcccctcg atgtgagctc gggacaggct tgctcgttgc
     7561 caattagccc accagcaagc ctgtcccgag cacggccgag ggaactgaca ggctacccat
     7621 gtttattact accggatcaa attttctagg acctctggaa ttctctgaat ggaatctgaa
     7681 gaatttcgca accattaagt cgaagtcact ttaaaaaggg tagcgtccct ttctctttaa
     7741 aaataaatcg ttgaagaaat aactcactat gacacatacg gaatttatta agcctcttga
     7801 agaaacaact ccattttgtt aaattaattg ttctaatggg aagtttcagt ggcaacctct
     7861 gatttaaaaa aagtattcgc attttcaaat ctatcaaaag cgtggaaatg gataaaatca
     7921 aatcccgaac caaattataa gaattatttc cgtccgattt atacagcata tgcggctgct
     7981 gaggattctt atctaaaatc cttacaactt cgt
//
LOCUS       DATDRL010000128         5659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2232676, whole genome shotgun sequence.
ACCESSION   DATDRL010000128 DATDRL010000000
VERSION     DATDRL010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2232676"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VI935_04625"
     CDS             <1..507
                     /locus_tag="VI935_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HLE24919.1"
                     /translation="EFLLGTLPESGNFPKRNRVISGLSLGVIIVQASDKSGSLITASL
                     ALEQNREVFAVPGNIGSKLSKGTNRLIKEGAKLVESVDDVLSEIEALRTLKEKEIKDN
                     SVSLTGLSHEERLIYTELKNESLHIDEITKLTGIASSKALTILLSLELSGLVTQLPGK
                     MFQIRKPM"
     gene            complement(815..3310)
                     /gene="gyrB"
                     /locus_tag="VI935_04630"
     CDS             complement(815..3310)
                     /gene="gyrB"
                     /locus_tag="VI935_04630"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HLE24920.1"
                     /translation="MERLMEGKQYKEDNSYTAEAIKVLPGLEAVRKRPDMYIGDTSTR
                     GFHHLVFEVLDNSVDEALAGFCDEVTVTIHADASVTVEDNGRGIPIDIHEETGKSAAE
                     VVMTMLHAGGKFDGKVYRVSGGLHGVGVTVVNALSEYLILEIRREGKVWYQKYERGEP
                     VSELKETGKTKRSGTKIRFKPDPEIFEVNEFNYDTLAHRIRELAFLNSGLRIKLLDER
                     GGREQEFYYKGGIVAFVQELNKNKNTLHPKPIYVSGEKDDIIVEVALQYHDGYTETIF
                     CYANNINNIEGGTHLTGFRGALTRTINKYAEDQGLFKNAKVVLSGEDTREGLTSVINV
                     KLPNPKFEGQTKTKLGNTEVKGIVETLLNDKLGTFFEENPSVARKIIEKTVEAARARE
                     AARKARELTRRKSALESGSLPGKLADCQERDPQYSELFIVEGESAGGSAKQARARFNQ
                     AVLPLRGKILNVEKARFDKMLSNEEIRTMITALGTGIGSDDFDISKLRYHRVILMSDA
                     DVDGSHIRTLLLTFFYRQVPEIIEKGYLYVAQPPLYRIKRGKEEKYLKDDHEFENHLL
                     HIGIEGVVLRVNRDGEYKDIKGDRLLDIIKKSISYGKILERLGMWGRDTSIVDAFASR
                     DSFRKSHLKIGNESELDEHLKGIKKWIEKRYLDISTLDWDLEEDEEHNCSRISYHFKE
                     NGRNRDTLIDFDFLNSPDFVELKNLRDTIKQVGDPPYFVLVSSSQSEEDGHREKQSGE
                     TKLGSLKEVTEYILTRGKKGMFIQRYKGLGEMNPEQLWETTMNPETRVLKQVRVEDAV
                     QADEIFTILMGDQVEPRKEFIEKNALNVRNLDI"
     gene            complement(3516..4070)
                     /gene="dcd"
                     /locus_tag="VI935_04635"
     CDS             complement(3516..4070)
                     /gene="dcd"
                     /locus_tag="VI935_04635"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006343078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HLE24921.1"
                     /translation="MGVKPDHWIRKMALNHKMIEPFVEEQITMGVISYGLSAYGYDIR
                     VSDEFKVFTNVYNTVVDPKGFDEKSFVSIKSNTCIIPPNSFALARTVEYFRIPRSVIT
                     LCVGKSTYARCGIIVNVTPFEPEWEGFVTLEISNTTPLPAKIYANEGIAQVIFFEADE
                     MCEVSYADKKGKYQKQIGITPPKV"
     gene            complement(4231..5208)
                     /locus_tag="VI935_04640"
     CDS             complement(4231..5208)
                     /locus_tag="VI935_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HLE24922.1"
                     /translation="MQVQNERSMETAKQHLVTKVPILREEESIGSVFARLPGTAFDSV
                     DAIYVVDELRHLSGFVTLPKLLVTPENQILREVMTKQPPAVNQDEDQERVVVLAIKHG
                     LTAVPVVDTEGRFMGVVPAHALIEILHHEHVEDLLRLAGIQRENAQARHAIDAPPVRR
                     ARDRLPWLLVGLLGSFFATFVVSRFGRVLEAYVAVSFFIPGIVYLADAIGTQTEAIAV
                     RGLSLSTATFRKSLTGELSTGFLIGLSLGGLSFPAIILAFGDIRLALAVSLTIVAAGT
                     IATTVGLLLPWILSHAGKDPAFGSGPVATIIQDVFSLIIYFIIIQLMVV"
     gene            complement(5255..>5659)
                     /locus_tag="VI935_04645"
     CDS             complement(5255..>5659)
                     /locus_tag="VI935_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HLE24923.1"
                     /translation="VTLLIGVNDQFQGYSEESYAINFEKLLIKAIELAGGRPYRVIVI
                     SIPDYSITPFGKRFGQARIRTEIDRFNEINKQIADEKGVHYVNVTEVSRKASESSTLL
                     APDGLHPSVQMYAEWLELIAPVAISVLDVNKQ"
BASE COUNT         1582 a         1338 c         1045 g         1694 t
ORIGIN      
        1 gaatttcttc tcggtactct ccctgagtcc ggaaactttc ctaaaagaaa tagggtcata
       61 agcggtctct cacttggtgt tattatagtc caggcttctg ataaaagcgg ttcacttatt
      121 acagcctctc tcgcactcga gcagaataga gaggtttttg ccgtgccagg aaatattgga
      181 agcaagctca gtaagggaac caataggcta ataaaagagg gagcgaagtt ggttgagtca
      241 gtagatgacg tcttaagcga gatcgaagct cttagaacac ttaaggaaaa agaaatcaaa
      301 gataatagtg tttctcttac cgggctatct catgaagaaa ggttgattta tacagagcta
      361 aagaatgagt cccttcacat cgatgaaatt acaaaattaa ctggaatagc ttcttcaaag
      421 gcacttacaa ttcttctatc tttagaactt agtggcctag ttactcagct tccaggaaag
      481 atgttccaaa ttagaaagcc gatgtaacct ttttctgctt taaataatca aacttaataa
      541 aatctatttg ctcttttttt cgaaattgga cgatgtcact tgacaaacaa aaatcactca
      601 caatttaaaa tagttaattt agtaattaac gaaaaccgca tttttggatt tattagagtt
      661 ctagatagaa cttagacata gattaaaaaa atttgttaat ccaaaataag gtataaaagg
      721 acttgactcc agctaaatgg actaagagga tacggaacta ataaataaaa aagagcctca
      781 ctacgaagct ctgtgttctc cataccaaaa cggactaaat atccagattt cttacgttga
      841 gggcgttctt ctcgatgaat tcctttctgg gctcgacctg atcacccata agtattgtaa
      901 agatttcatc agcctgaacc gcatcttcaa cccttacctg tttgagcaca cgggtttcag
      961 ggttcatcgt ggtttcccat agctgttcgg gattcatttc accaagcccc ttatacctct
     1021 ggataaacat ccccttcttc ccacgagtga gaatatattc cgtaacctct ttcaggcttc
     1081 caagcttcgt ttctccactt tgtttctctc tatgaccatc ctcttcagac tgagaacttg
     1141 atactaaaac gaaataaggc gggtccccga cttgcttaat ggtatcacgg aggtttttga
     1201 gctcgacaaa atccggtgag ttcagaaaat caaagtcaat tagtgtgtct ctgtttctac
     1261 cgttttcttt aaaatgatag ctaatccttg agcagttatg ctcttcatct tcttccaaat
     1321 cccaatcaag ggtcgaaatg tcgagataac gtttttcaat ccactttttg atccccttta
     1381 gatgctcatc gagttctgac tcgtttccaa tcttcagatg actctttcta aagctatcgc
     1441 gactggcaaa tgcatcaact atgctagtat cccttcccca cattccaagc ctctcaagaa
     1501 ttttcccata tgatatagac ttttttataa tatcaagaag tctatctcct ttgatatctt
     1561 tatattcacc atctctattt actcttaaaa caactccctc aatcccgatg tgaaggagat
     1621 gattctcaaa ctcgtgatcg tccttaagat acttttcctc tttaccgcgt ttaatcctgt
     1681 aaagcggagg ctgagccacg tatagatacc ccttctcaat tatctctgga acctgcctgt
     1741 aaaagaatgt aagaagtagt gtcctaatat gtgagccatc tacgtcagca tcgctcataa
     1801 ggattactct atgataacgg agtttcgata tatcaaaatc atcgcttcca atcccagtcc
     1861 caagtgcggt aatcatagtc ctgatttctt cgttacttag catcttatca aaccttgctt
     1921 tctcaacgtt tagaatcttc ccacggagcg gtaggaccgc ctgattgaac cttgcccttg
     1981 cctgctttgc tgaacccccc gcagactctc cctcaacaat aaaaagctca gaatactgtg
     2041 ggtcacgctc ctgacaatcg gcaagctttc ctgggagtga acccgactcg agcgcactct
     2101 ttcttcttgt aagctcgcgc gcctttctcg ccgcttctct tgcccttgca gcctctacgg
     2161 tcttttcaat tatttttcta gcaactgagg gattttcctc aaaaaaggtt ccaagcttgt
     2221 cgttaagaag tgtctctaca attcctttaa cctctgtatt tccaagctta gtctttgtct
     2281 gtccttcaaa tttaggattc ggaagcttta cgtttataac cgatgtaaga ccctctctcg
     2341 tgtcttctcc acttaataca acctttgcat ttttaaaaag tccctggtcc tcagcgtatt
     2401 tatttatagt tcttgtaagc gcacccctaa atccagtgag atgtgtacca ccctcaatgt
     2461 tattaatgtt atttgcataa cagaatatgg tttctgtata tccatcatgg tactggagag
     2521 caacctcaac aataatgtca tctttttccc cagaaacata aattggtttt ggatgaagcg
     2581 tgtttttatt cttgtttagt tcctgaacga atgcaacgat tccccccttg taataaaatt
     2641 cctgctccct tccaccacgt tcatctagaa gctttattct taagccgcta tttaaaaaag
     2701 caagctccct gatcctatgg gcaagcgtat catagttgaa ttcattaacc tcaaaaatct
     2761 cagggtctgg cttaaatcta atttttgttc cgcttctttt tgtctttcca gtctctttta
     2821 attcactaac tggttctcct ctctcgtatt tttgatacca gaccttaccc tctcttctta
     2881 tctcaagaat taggtactcg gaaagagcat ttacaactgt gaccccaacc ccatggagac
     2941 cacctgaaac cctatagacc ttgccgtcaa attttccacc ggcatggagc atagtcataa
     3001 ccacttctgc agcggatttc cccgtctctt catggatgtc tatagggata ccccgaccat
     3061 tgtcctctac agttacgcta gcatctgcgt ggattgttac agttacctca tcacaaaacc
     3121 ctgcaagagc ctcatcaacg ctgttatcaa gcacctcaaa aacgaggtga tgaaaacccc
     3181 ttgtgcttgt atcacctata tacatatctg ggcgttttct tacagcctct aaccccggaa
     3241 ggaccttaat cgcttctgca gtatatgaat tatcttcctt atattgcttt ccttccatta
     3301 atctttccat ttctaaacta gaccacctcc gttaataaat tggaagctta taaatgttac
     3361 cataaaacac gctaaacgta aagaaaatca aggggtttcg tggcgtcaca aatggtaggg
     3421 gcacgttgca acgtgtccct accaagtggc taggtggtaa cagcaagact cgttttccta
     3481 cacacagttg cactatcaat gtaatagttt ttgatttaaa cctttggcgg tgtaatacct
     3541 atctgctttt gatatttacc cttcttatca gcataagata cctcgcacat ttcgtcagcc
     3601 tcaaagaata taacctgagc tattccttca ttggcgtaaa tcttggctgg aagcggtgtt
     3661 gtatttgaaa tctctagtgt tacgaaccct tcccactctg gttcgaatgg ggttacgttt
     3721 actattatcc cgcagcgggc gtatgtggat tttccaacac aaagcgtaat cacacttcta
     3781 ggaattctga aatactcaac agttcttgca agtgcaaatg agtttggagg aatgatgcaa
     3841 gtattggatt taattgatac aaaagacttt tcatcgaatc cctttgggtc aacaacagtg
     3901 ttataaacat tcgtaaaaac cttgaactca tcgcttaccc ttatgtcata cccataagct
     3961 gataagccat aagaaataac acccattgtt atctgttctt cgacaaatgg ttctatcatc
     4021 ttatggttga gagccatttt tcttatccaa tgatcgggtt tcacacccat gtcaactttg
     4081 ctcctttcaa tatgatataa aaataaaagt gatgctacca tacttaggaa tgttactcaa
     4141 cccatacata agtaatcaca taagtaaacg aaagatatcc tcggatcaac ttcttacctt
     4201 gtcaatgaca aaagtaggtg caagcatcgt ttaaacgacc ataagttgaa taattatgaa
     4261 atatatgatc aaactaaaca catcctgaat aattgtagcg acagggcctg agccaaacgc
     4321 tggatccttg ccagcatgag atagtatcca cggtagaagc agaccaacgg ttgttgcaat
     4381 cgtcccagca gcaacaattg tcagagatac agcgagggcg aggcggatat cgccaaaagc
     4441 caggattatg gcaggaaagc tgaggccccc cagtgaaagc ccaataagaa atcctgtgct
     4501 taactcgcca gtaagagact ttcgaaaagt agcagtgctc aatgaaaggc cgcggactgc
     4561 aatcgcttcc gtttgcgttc cgattgcatc agctaaatac acaatgcccg gaataaagaa
     4621 tgagacagct acgtaggctt cgagaactct cccaaatcgt gaaacaacaa acgtagcaaa
     4681 gaagctaccg aggagaccta cgagaagcca gggaagccga tcacgagccc ggcgcaccgg
     4741 tggagcatca atcgcatggc gggcttgagc gttctcccgc tgaatacctg cgagccgaag
     4801 gagatcctca acgtgctcat ggtggaggat ttcaatcagc gcatgtgctg gaacaactcc
     4861 cataaaccgt ccttccgtat caacaactgg gacggccgtc aagccgtgct tgattgctag
     4921 tacaacaact cgttcctggt cttcgtcctg attgactgcc ggcggttgct tagtcattac
     4981 ctcccgtagt atctgattct ctggtgtaac tagaagttta ggcaaggtta caaacccgct
     5041 caaatgccgt agttcgtcaa cgacataaat ggcgtcaact gaatcaaatg ctgtgcctgg
     5101 gaggcgtgca aagacagatc caatgctttc ttcttcgcgg agtattggaa cttttgtgac
     5161 aagatgctgc tttgcagtct ccattgatcg ctcattctga acctgcatcg cttaccctcc
     5221 ttcgcggcgt aatttgacag aatgccacca tgttttattg cttgtttaca tcaagcacac
     5281 ttattgctac tggggcgatc aattccaacc attcggcata catttgtaca gacgggtgga
     5341 ggccatcggg ggctaaaaga gtcgaacttt ctgaagcctt tcgggatacc tctgtgacgt
     5401 ttacgtaatg gacacctttt tcgtccgcga tttgcttgtt gatctcatta aatctgtcta
     5461 tttcggttct gatccttgct tggccaaacc gcttgccaaa cggtgtgata ctataatcag
     5521 gtattgatat tacaatgacc cgataaggcc ttcctcctgc taattcaatt gctttaataa
     5581 gcaatttctc aaaattaata gcgtaacttt cttcgctata accctggaat tggtcattta
     5641 ccccgataag caatgtaac
//
LOCUS       DATDRL010000129         5730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2653475, whole genome shotgun sequence.
ACCESSION   DATDRL010000129 DATDRL010000000
VERSION     DATDRL010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2653475"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /gene="prs"
                     /locus_tag="VI935_04650"
     CDS             complement(<1..514)
                     /gene="prs"
                     /locus_tag="VI935_04650"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HLE24924.1"
                     /translation="MEHTKIFSGTSNIPLTNAVCKYLDVLPGKAEIRRFSDGEIFAEI
                     SESVRGSHVFVIQSTCPPVNEHIMELLIITDALKRASARVITAVIPYYGYARQDRKVQ
                     PRAPISAKLVADIIVKAGINRVVTVDLHAGQIQGFFDVPVDHLYAAPVFISHLKKKFN
                     KDEIVVVSPDA"
     gene            634..1065
                     /locus_tag="VI935_04655"
     CDS             634..1065
                     /locus_tag="VI935_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HLE24925.1"
                     /translation="MIKPTDKEIEFIKSHRVARMATVDGLGRPLVVPICYYFNGSSIY
                     TPIDKKPKRASPQELKRVRNIIENPNASLVIDEYYEDWTKLCYVIINGKAEIMESGEE
                     YQNSLKLLSEKYTQYAEMKLSGLNLPVIKIIPERIISWGRF"
     gene            1068..2372
                     /locus_tag="VI935_04660"
     CDS             1068..2372
                     /locus_tag="VI935_04660"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLE24926.1"
                     /translation="MTRLVVLGSGFGGMEALLTLESRLGKRKDVEIILVSNQNHTLFT
                     PLFPQIVSSYIEPRHIIQTIRDIRRDKKFRFIRDSVIGIDLNDKLVSLSEGELSYDYL
                     VIALGGTTNYFNVPGAESYTFTLKTLQDSAELRDHVIDILEHADHEHDLSLKKEMLTF
                     VIVGGGYTGVELTTELRDFIYRYVVKTYKGITLNDVRIVLIEASGEILEGVNPELAKR
                     AKTKLLKGGMEVRTDSKVTKCIEDGVEINNQYVFSARIVIWTAGVRANPISDSLTVRK
                     GKFGRILVNDYLQIPEFPGAYSIGDIAIVEGENPAKSSQPIAPVAIQQGRIASRNIVN
                     SIENRPLEKYLFSPSGILISLGMNDAVTNIKGFKFVGFIAWLLWNAVHLLKLVGLKKQ
                     LQVALDWSLGSIFPRDSAIIRFPQRCRICAGKRGVQNKKYQY"
     gene            complement(2822..4300)
                     /gene="glgA"
                     /locus_tag="VI935_04665"
     CDS             complement(2822..4300)
                     /gene="glgA"
                     /locus_tag="VI935_04665"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HLE24927.1"
                     /translation="MKVLFVSPEMEPLAKVGGLADVVGALPKELENLGCDVRVIIPLY
                     KQVKENLINLKLKAKNLRKEIIVCLDWLPFRGDVMEVDLNGLTVYLLRNDNFFNREYI
                     YSTPQGDYQDNDLRFGFLSLGALEIAKALDFKPDIIHCHDWQTALVPITLKWRRHVKG
                     DPFFSESKIVFTIHNISYQGLFPREILEKLGLPLYAFTPQGIEFYGKVSLLKGGIVYG
                     DAVTTVSPNYAEEIKKPEYGNALDGILRWISQESNKLVGILNGIDYDAWDPKTDKALY
                     SNYGIGDINGKLKNKSELQNELGLYADEGKPLLGMVSRLTEQKGIDLVVEALPLIFDL
                     GFQVAILGTGEEKYVRMLENAKSRYKGNLSVVIGFNDEIARRIYAGSDMFLMPSRFEP
                     CGLGQMIAMKYGSIPVVRGTGGLLDSVRDYSVDRKHGNGFIFHLFSKVSFLDALTRAL
                     LVYENHEVWIGLVTRAMKEDFSWKRSSEKYLELYKRLKGSNP"
     gene            complement(4304..4921)
                     /locus_tag="VI935_04670"
     CDS             complement(4304..4921)
                     /locus_tag="VI935_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HLE24928.1"
                     /translation="MDFIRKNKVFAVIRAEDTEDAIRFANACIEGGLKLIEITFSFPG
                     ADGVIKELSKIDDVIVGAGTVLNIDMAKTAATSGARFLISPHTDKEIISYAKSHELLV
                     VTGTLTSSEILNAWNMGVDLVKIFPVKAVGGASYIQAIKETLPFIEIMTTGGVTVENF
                     TEFLKAGATAVGLSSSLIGSDRFNDPKAIAQTAKVVIQRLRELEE"
     gene            complement(5504..>5730)
                     /locus_tag="VI935_04675"
     CDS             complement(5504..>5730)
                     /locus_tag="VI935_04675"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HLE24929.1"
                     /translation="RQLSSHVTQSNASKYGVKILADWVYEPEHTVILVLEADSPEKAT
                     NFALPFLNVGSITIRAGATCEDVAKMCLGK"
BASE COUNT         1676 a         1200 c         1142 g         1712 t
ORIGIN      
        1 cagcatctgg tgatactacg actatttcat ctttattgaa tttctttttt aaatggctta
       61 tgaaaacggg agcagcgtaa agatgatcaa cgggaacatc gaaaaacccc tgaatctgtc
      121 ctgcatgaag atcaacagtt acaactctgt ttataccagc tttaacgata atatctgcaa
      181 cgagttttgc tgagattggg gcccttggtt gaaccttcct atcctgcctg gcatagccat
      241 aatagggaat aactgcggta attacccttg ctgatgccct ttttaatgca tctgtgataa
      301 taagaagttc cattatatgc tcatttacag gcggacaggt tgactggatg acaaagacat
      361 gtgatcctcg aacgctttct gagatctctg cgaatatctc accgtcgctg aacctcctta
      421 tctctgcctt cccaggcaaa acatctaggt atttgcaaac agcatttgta agaggaatat
      481 tagatgtacc actaaatatc tttgtatgtt ccataggatt ttactgaaaa taaaagcgct
      541 gaaagacttt aagtaacaaa gggattatta taaaatactt cattaataat tcaagtttga
      601 tggtaagtac cttaacactt ataaaacgtc cttatgatta aaccaactga taaggagatt
      661 gaatttataa aatcacacag ggttgctagg atggcaacag tagacggatt aggaaggccg
      721 ttagtagtcc ccatatgcta ctatttcaat ggaagctcaa tttacacacc aatagataaa
      781 aagccaaagc gcgcctctcc acaagagcta aagagggtta ggaatataat tgaaaatcca
      841 aatgcatcgc ttgtaattga tgagtattac gaggattgga ccaagctctg ttacgttatt
      901 attaatggga aggctgagat aatggagtcg ggggaggaat atcagaattc attaaaacta
      961 ctctctgaaa agtatactca gtatgcagaa atgaaactat ctggattaaa ccttcctgta
     1021 attaaaatca ttcctgagcg gataatttct tggggtaggt tctaaaaatg actaggttag
     1081 ttgtcttggg ttctgggttt ggagggatgg aggctctctt aaccctcgaa tctaggcttg
     1141 gcaaaagaaa ggatgtggaa ataattcttg taagcaatca aaatcatacg ttattcaccc
     1201 cattgttccc tcagattgtt tcttcataca tagagccaag acacataatt cagacaataa
     1261 gggatatcag aagggataag aaatttaggt ttattcgaga ttcagtaatt ggcatagacc
     1321 ttaatgataa attagtaagt ctttcagaag gcgaactctc ttacgattat ctggttattg
     1381 cactaggtgg cacaaccaat tacttcaacg tgccaggagc tgaaagttat acctttactc
     1441 tcaaaaccct acaggattct gcagaattaa gagaccacgt catagacatt cttgagcatg
     1501 ctgaccatga gcatgattta agtttaaaaa aggagatgct cacatttgta atagtaggcg
     1561 gcggctatac aggggttgag ttaacaacag agttgagaga ttttatctat cgttatgtcg
     1621 taaagaccta taaagggata actttaaacg acgtaagaat agtgctaatc gaagcaagcg
     1681 gagaaattct tgagggtgtt aatccagagc tagctaaaag ggcaaaaacg aagctcctta
     1741 agggaggcat ggaggttagg acagattcta aggttacaaa gtgtattgag gacggcgtgg
     1801 aaattaataa tcaatatgtc tttagtgcaa gaatagtgat ctggacagca ggcgtaagag
     1861 caaaccctat atcagattcc ttgacggtaa gaaagggaaa atttggaaga attctagtaa
     1921 atgattacct gcaaatacct gaattcccag gtgcttattc tattggggat attgccatag
     1981 tagagggtga aaacccagca aaatcatctc agccaatagc tcccgtagca atccaacagg
     2041 gaaggatagc gtcaaggaac attgttaatt caatagaaaa tagaccttta gaaaaatacc
     2101 tattctctcc atcgggaatc cttatttctc ttgggatgaa tgatgcggtg acaaacatca
     2161 aaggatttaa gtttgtgggt tttattgcat ggttactctg gaatgcagta catttactta
     2221 aactcgtagg tctcaaaaag caactccagg ttgcgctcga ctggtctctt ggctcaattt
     2281 tcccaagaga ttctgctatt atcagattcc cgcaaagatg taggatttgt gctggaaaga
     2341 gaggagtaca aaacaaaaaa taccaatatt aatcattata aaatttctcg ttattaattg
     2401 gttaataata ttatccttga cggggttaga aaaccccacc tatcgtgtta tttgcataga
     2461 tataggggca ggctttgtcg tgagcccttc gtcaaggctc aggacaggct cagccgaacg
     2521 gtcttgcacc tgcccttgtt ggggaaccgt ggctcaactc agcttgccac aggcaagggt
     2581 tgccccaaca attggggaac gatttgcctc ctagcccacc agcaagaccg gtggtttttg
     2641 ttttacataa ccgcctcttg ggttgccaac gagtcttgct cgttggctat tatacccgcc
     2701 agcaagactg gtggcctatg tttattatca ggttaatttg tcaattaact aattatttgg
     2761 attaaggagc taaaaaaatg tagcggagtg cttaggccct ccataaatag aatcctcaat
     2821 attagggatt gcttcctttc aacctcttat atagctccag atatttttca ctcgaccttt
     2881 tccaggaaaa atcctctttc attgctcttg tcacaagccc tatccacacc tcatggtttt
     2941 cgtataccaa aagcgctctt gtaagagcat cgagaaaact caccttcgaa aagagatgaa
     3001 atataaatcc gtttccatgt tttctatcaa ccgaatagtc cctaaccgaa tccaagagac
     3061 cgcctgttcc tcttacaaca ggaatgcttc catacttcat ggcaatcatc tgcccaagtc
     3121 cgcatggctc aaatcgtgaa ggcatgagga acatatcaga gccggcatag attcttcttg
     3181 cgatttcgtc attaaaacca ataaccaccg atagatttcc tttatatctt gacttagcat
     3241 tctcaagcat acgcacatat ttctcctccc ctgttccgag aattgccacc tgaaatccaa
     3301 ggtcaaaaat cagtggaaga gcctcaacga caaggtctat gcccttttgc tctgtaagcc
     3361 tcgataccat tccgagaagc ggttttcctt cgtcagcgta aagccctagc tcattttgta
     3421 gctcggattt attttttaat ttaccattaa tgtcgcctat tccgtagttt gagtaaagag
     3481 ccttatctgt tttgggatcc catgcatcat agtcaattcc gtttaaaata cctactagct
     3541 tatttgattc ctgtgaaatc catctgagaa taccgtccaa ggcatttcca tactcaggct
     3601 tttttatctc ttcggcatag ttagggctta cggtagtaac tgcgtctcca tagactatgc
     3661 cgccttttag aaggcttacc ttcccataga actcaatgcc ttggggtgta aatgcataca
     3721 aggggagtcc aagtttctct aagatctctc ttggaaataa tccctgatag gagatattat
     3781 gaattgtaaa aacgatttta gattccgaaa agaaaggatc gccttttaca tgtcttctcc
     3841 acttgagagt aattggaaca agtgccgttt gccagtcatg gcagtggata atatctggct
     3901 tgaaatcaag tgcttttgct atttcaaggg cgcccagaga aagaaatcca aatctcaggt
     3961 cgttatcttg ataatcaccc tgaggcgtac tgtatatgta ttcacggtta aaaaaattat
     4021 cattcctgag taaataaacg gtaagtccgt ttaaatcaac ctccatcaca tcgccccgaa
     4081 aaggaagcca atcgaggcaa actattattt ctttccttag attctttgct tttagtttaa
     4141 ggtttattag attctctttt acttgcttat aaagcgggat aattactctc acgtcacatc
     4201 cgaggttttc aagctccttt ggaagggcac caacaacatc agcaagccct cccactttag
     4261 caagcggctc catctcaggc gaaacaaaaa ggactttcat gaattattcc tccaattctc
     4321 tcaacctttg aattacaacc ttcgctgttt gagcgatggc cttaggatca ttaaatctat
     4381 cgcttcctat caaactactt gaaagaccga cagcagtggc tcctgcttta agaaactctg
     4441 taaaattctc tactgttact ccgccagtcg tcataatttc aataaaagga agtgtttctt
     4501 ttattgcttg aatatacgat gctcctccta cggctttaac aggaaagatt tttactagat
     4561 ccactcccat gttccatgcg tttaggatct ctgaagatgt aagtgtccca gttacaacta
     4621 gaagttcatg agatttagca tacgagatga tttctttatc tgtgtgtgga gagattaaaa
     4681 accttgcgcc tgaagtggct gcagttttcg ccatatctat attgagcacc gttcctgctc
     4741 ccacaataac atcgtctatt ttgcttaact cttttatcac accatccgct ccaggaaagg
     4801 aaaatgtaat ctcaattaat ttaagaccgc cttcaataca agcgtttgca aatctaatag
     4861 catcttcggt atcctcggct cggattacag cgaatacctt gttttttctt ataaaatcca
     4921 tgatgttcat cttaagaccc gaattaaatt actcatacat cattttgttt tcaagtttta
     4981 acttgataat tccctttagt ttactgcagg gtttccaccg acattgcgaa cacattcgct
     5041 gagcctgtgc tgtggaaacg gaagtgctta gtgtaaactc tgtgaggcga tacctttgag
     5101 attcctcgct gcgctcggaa tgacaactag tgtaagattg cttcgtcacg gagtttatcg
     5161 ttcggctgag ggttcgcccg ggctcagccg aggcgctcac ggcgaagtcc tgagcgattt
     5221 cgccaaggat tcctcgcaat gacacttccg tgaagtcaat gcaacgacta aagagactat
     5281 cttttccatg ctaatacccc gcaccttggt ccggtttctt gattgatgtt tacaggaatt
     5341 tgaatgtttt actttacgtg agttgagatc acacttggtt cagatttgat acacctctct
     5401 ggaatgctag ggttggcatt catatgacac gcttgaaaat ccctacttac agaactgcca
     5461 gatgctttta ttattagatt tactgaattt tgtatagcgt acttcacttg ccgaggcaca
     5521 ttttagcaac atcttcgcaa gttgcaccag cccttattgt aattgatcct acgttaagaa
     5581 atggtagtgc gaagttggtt gccttttccg ggctatccgc ttctagaaca aggataacag
     5641 tgtgctccgg ctcatatacc caatcggcta agatcttaac tccgtacttg ctggcattag
     5701 actgtgttac atggctagac agttgccgca
//
LOCUS       DATDRL010000130        11814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3751584, whole genome shotgun sequence.
ACCESSION   DATDRL010000130 DATDRL010000000
VERSION     DATDRL010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11814
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3751584"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VI935_04680"
     CDS             complement(<1..183)
                     /locus_tag="VI935_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24930.1"
                     /translation="MKSKNKYLFLIIFLGICVLLVGGGYLVAFPTIPQPKNSNTNTVF
                     WDAKLSHPYFHKGSDDD"
     gene            complement(180..839)
                     /locus_tag="VI935_04685"
     CDS             complement(180..839)
                     /locus_tag="VI935_04685"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HLE24931.1"
                     /translation="MGFKNRINVLLKSQFNYWVTQAEDPEKIINHSVEEMEEGLDRAR
                     AKLATLKFRVDGERRFLQRIGEQISYLQGRTEELLKEDMEENAKEAVRRKRILEEEER
                     KLKIKHAEDEVKLKEMQAALKELENRVQMAKAKRNILIKDIRLRKGIGEGTKEEHMGI
                     DFEEPFSLLRKMEERVEEDTEFTYSRQLKEKEALEKEERLINEEVEMLKRNIEKGGSK
                     K"
     gene            complement(963..1181)
                     /locus_tag="VI935_04690"
     CDS             complement(963..1181)
                     /locus_tag="VI935_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HLE24932.1"
                     /translation="MGKLSPVSWKQLVSKLKSFGFDGPYSGGKHPFMIKGDLVLSLPN
                     PHRKQIGVDLLSRILKVAGISRHEWVKS"
     gene            complement(1181..1423)
                     /locus_tag="VI935_04695"
     CDS             complement(1181..1423)
                     /locus_tag="VI935_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002787538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLE24933.1"
                     /translation="MLTKYIRVAMEKAHYEILEESKTYYGNIPGFKGVWAEGKTLEEC
                     REELQEVLEEWIMIRLRRGLSLPVLKGVELKISTVA"
     gene            complement(1692..2915)
                     /locus_tag="VI935_04700"
     CDS             complement(1692..2915)
                     /locus_tag="VI935_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HLE24934.1"
                     /translation="MNCERFEELLIDFMDNEIEPSERELVKKHLAACPRCYRRLEEYQ
                     EIRRIFNEETIPQPSPQVLATLSKRARQEVAKDKSTFWKRWFYSPILVPVLSSALALS
                     IWVYYGQKNISYSPGETIYSRDAMAKKVPIAQQPNIPVSGNKVLDKIGSEKHAILSKP
                     LERGSNAPPSRGNILEGIESKPGSVLSSKPYSIPSRAAGKQETIQEKSDTSLRVTPGE
                     GLVVEKDLKVTGKTSEEMASARLEDKSMNEPVSIPPVKTEVEALQREELAEGQFEEQA
                     EQEIREDKEKSKEAKLYAYRGSAYNEQLNLALKQQNEGNCEASIKTNEELLKISPPPP
                     DSVKEKAYLSLAGCYEQKGDLENAISNYNNLQVVAPVQTTFAKVKIEALRQKITQLKA
                     RELVPSDTEKGQKSK"
     gene            complement(2924..3577)
                     /locus_tag="VI935_04705"
     CDS             complement(2924..3577)
                     /locus_tag="VI935_04705"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLE24935.1"
                     /translation="MFGLVAKKELADEEAMVRFQNGDARAFDLLLHRHSTGVLRFIIK
                     MIGSSGRLQRAPTQAEDLLQEVFIKVIENKEKYDSSQKFTAWLYTLTRNCCIDHLRTE
                     NHRHHDSLDASLSRDEINGAVVLDIIKSGERNQEEAIIDKEIQRLLDSGIEELREEFR
                     EVFLLREIEGLSLKEIADITDTNLSTVKSRLRYAYQNLREVFIRAGYFGERQRVKEV"
     gene            3917..4117
                     /locus_tag="VI935_04710"
     CDS             3917..4117
                     /locus_tag="VI935_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24936.1"
                     /translation="MNKDRKPEIYAEYTRIQEELWKDYEEIRDESINGYLRIKLQMCY
                     KEFKLLEKSLRDKYYRGKKRIA"
     gene            4382..4795
                     /locus_tag="VI935_04715"
     CDS             4382..4795
                     /locus_tag="VI935_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HLE24937.1"
                     /translation="MKSHTEYLWFNTKNRKEIIHITDKVRETVKKSGVEEGFVLISAM
                     HITAAVYVNDLEEGLFEDIWEWLEELAPYRKDYKHHRTGEDNGDAHLKNLMMHHQVIV
                     PITKGDLDLGPWQRVFYAEFDGQRRKRVIVKVIGE"
     gene            4801..5262
                     /locus_tag="VI935_04720"
     CDS             4801..5262
                     /locus_tag="VI935_04720"
                     /inference="COORDINATES: protein motif:HMM:NF022609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HLE24938.1"
                     /translation="MKEPEEKKDKFQPRIYIDKEGNWYQDGIPILHRWTYLYNNKLLR
                     RDEEGRYYVDEGSGRVYVYVEDTPFVVKMIDKRQEGFFIILNDETEEKLDFNALWLNN
                     ENIPYTRVKNGEFEARFSRPAYYEFTKYLKQEGDRFYIELDGTKYDLNAKK"
     gene            complement(5392..5682)
                     /locus_tag="VI935_04725"
                     /pseudo
     CDS             complement(5392..5682)
                     /locus_tag="VI935_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018624064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(5951..7114)
                     /gene="hemW"
                     /locus_tag="VI935_04730"
     CDS             complement(5951..7114)
                     /gene="hemW"
                     /locus_tag="VI935_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HLE24939.1"
                     /translation="MTFGAYVHIPYCVKKCPYCDFNSYGVGRRIPEKEYTEAVLKELD
                     FYRESIENLPLTSIFFGGGTPSLFSADSIGKIISKILNVTTPLDSIEISLEVNPKTAE
                     LEKLKALKDVGVNRMSVGVQSFSERKLKLLGRINTPDDSRRVLEDIIKAGFNNFNLDL
                     IFGVSFETLDEWMGDLEKALDFNTIHISAYCLTIEDDTGFGALYSQGKLPLPDEDTLA
                     EMLIFTSDFLEQAGFVQYEISNFAKQGHECRHNLLYWRGENYLGLGAGAHSHLDLNEG
                     SSWGVRWANLKNPDLYMRTVLEGKIPLAFTELLSREEAIQDKIMMGLRLGEGINLTSM
                     RDRFGAKLYSDSLSALFKQGFLELSDNSLHLTKKGILVSNELIMKVCDSLVYD"
     gene            7220..8452
                     /locus_tag="VI935_04735"
     CDS             7220..8452
                     /locus_tag="VI935_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLE24940.1"
                     /translation="MRALLLLLILSSSTLSIPSLAQEITETLTLDKALEIALEKNPTL
                     EASRASVDILKARVQGVRSDLFPQIESRFIYPFVGTESGVSLDQLIWDFGYTSNIIKA
                     TKAQVKSSEYDKQANREDLILDIKVAYYTVLAQKRIVEASEKKVIENERRLEQAEGFF
                     KVGRVSKIEITKAKVNLGNAKLNLVTAKNNLERARLQLATAMGIIGDFNYELEDMLEY
                     KMIDLNLEQAIDKAFELRPELDSLEAKKVAMKANLKASKQDYYPSIFGRTAYRIKGEG
                     ATQPAFIAGIGVQFPIFEGFSSLSEVNEAKANLRRSQAEIQSMKGQILSEVKQSYLNL
                     QLSEESIKVTETSKTSSEENLELARERYRLGRASSIELAEAEALYASTNADYIQAIYN
                     YKIAVAQLERASGEKLEE"
     gene            8449..9612
                     /locus_tag="VI935_04740"
     CDS             8449..9612
                     /locus_tag="VI935_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLE24941.1"
                     /translation="MKILDKKTVVIIAILLIVVVYLAYRIFQNEATSIAYKTAKVDKG
                     DIISYVTATGTLNPISTIEIGSQVSGTIKNIFVDFNSVVKKGDPLSEIDPTPFNSQLK
                     QAHADLKKAEADAEIAKTLQRANEVLYKKRLISKEEYDDSRAKYSSAIATLEQRKAAL
                     EITKANLDNTSIRSPIDGIVISRKINISQIVTSGQSSSPLFLIAEDLSRMKLDAHVSE
                     ADIGRVKNEQEAEFTVDAYPNQTFKGKVWQIKNEPITTNNVITYDVVILIDNNDLKLK
                     PGMTAEVKILVAHKRDVLRVPTAALRFIPPPYAPVDEGFIELNNTSVVWVPLKNGRLK
                     AVSINPGVSDEAFTEILDGNLGEGEKVIVEATENTGTGSEPLGPIVLPKPQRF"
     gene            9617..10336
                     /locus_tag="VI935_04745"
     CDS             9617..10336
                     /locus_tag="VI935_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLE24942.1"
                     /translation="MEDIIVVENLHKVYKMGDVDVHALRGITLTIKKGGFISVIGPSG
                     SGKSTLMNIIGCLDKPTSGRYLLEGNDISSLDNDELADIRNKKIGFVFQNFNLLPRST
                     AIENVELPLIYNGIPSEKRYRLAKEALFMLGLEGREKHYPTQLSGGQQQRVAIARAII
                     NKPSIILADEPTGNLDTNTSIELMEIFKRLNKDGKTIIMITHETDIAAYAERVVMFRD
                     GKIMKEELRSKEKLHEPVSRL"
     gene            10314..11528
                     /locus_tag="VI935_04750"
     CDS             10314..11528
                     /locus_tag="VI935_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLE24943.1"
                     /translation="MNPLVAFRVSYRALRRNKVRSILTTLGIIIGVAAVITMVSLTQG
                     AKRIIEEQLTSLGGNSLIINPGSRTGSGVTKGLGKTNTLPSEDAEAIRKLSTVIHVSP
                     ILDTTAQVVWGNRDWFTALVGVSPDFVFINGWFPERGNFFHNEDVKNAELVCVLGKTV
                     ASKLFGYQNPLGKTIRIGNISFKVIGVMSTIGQTPGGKDQDDVVLIPYTTLQKRILGV
                     NHVDNISVSVRSSKDIPFAEAQITELLRERHRIRPNMEDDFYVKTQLNIIERIFTISK
                     IMTILLGSIASISLIVGGIGIMNIMLVSVTERTREIGIRMAVGAKERDILIQFLIEAV
                     VLSLTGGVIGIVLGIVGSKISSFFTHWPTLVSFGSILLAFGFAAIVGIFFGLYPASKA
                     SKLDPIEALRYE"
     gene            complement(11532..>11814)
                     /locus_tag="VI935_04755"
     CDS             complement(11532..>11814)
                     /locus_tag="VI935_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24944.1"
                     /translation="IPTDPDFAIGAPGTAEGGSVYVFFGSDDGFPASVLASSCAMEAS
                     LCLPPLNGMSQGFLADDKFGSIVLGVGDISGDFINDVAASGLGFVRAEF"
BASE COUNT         3681 a         2352 c         2244 g         3537 t
ORIGIN      
        1 atcgtcatct gagcccttgt ggaaataagg atggcttaat ttagcatccc agaaaactgt
       61 attcgtgttt gagtttttag gttgtgggat ggtaggaaag gctactaagt acccgcctcc
      121 tactagaagg acgcaaatac ctagaaaaat aatcaaaaac aagtatttat ttttactctt
      181 cattttttac ttcctccttt ttcaatattt cttttaagca tttcgacttc ctcgttgatt
      241 aacctctctt ctttttccaa ggcttctttc tctttaagct gccttgaata ggtaaactcc
      301 gtgtcttcct ctaccctttc ttccatctta cggagtagag agaacggttc ttcaaaatca
      361 attcccatat gctcttcttt tgtcccctcc cctatacctt ttctaagtct tatatcttta
      421 ataagaatgt ttcttttagc ctttgccatc tgaactcggt tttccagttc cttaagcgca
      481 gcttgcattt ctttaagttt cacttcatcc tctgcatgct tgatctttag ttttctttct
      541 tcctcttcga gaatcctttt tctccgcact gcttcctttg cgttttcttc catatcttct
      601 ttcagcaatt cttcagtcct tccctgcaag taagaaatct gctcaccgat tctttggagg
      661 aatcgccttt ccccatccac tctaaatttg agcgtagcaa gttttgctct tgccctatcc
      721 agcccctctt ccatctcctc aactgaatga ttgattatct tttccgggtc ttcagcctgt
      781 gttacccagt aattaaactg ggatttaagt agcacgttta ttcgattctt gaatcccatg
      841 ttaattccct cttatttagt tttaggtatt aatacgaatg agatttagga aggatttaag
      901 atggagagaa aattaatgaa atattttaag cagaaatgaa cacaaatgaa aagaggttac
      961 tcttagcttt ttacccattc atgcctagat atgccagcta cttttaaaat tctacttagt
     1021 aaatcaacgc caatttgttt tctatgaggg ttgggcaatg atagcactaa atcccctttg
     1081 atcatgaagg gatgtttacc accagaatat ggaccatcaa acccaaaaga ttttagtttg
     1141 cttactaatt gcttccatga tacaggcgaa agcttaccca ctatgctact gtgctgattt
     1201 taagttcgac tccttttaga actggcagtg aaagtcctcg tcttagcctg atcataatcc
     1261 actcttcaag tacttcctgc agttcttctc ggcattcctc tagtgtcttt ccttcagccc
     1321 agaccccctt aaatccaggg atattaccat aatatgtttt agattcctca agaatttcat
     1381 aatgagcttt ttccatagcg acccttatat attttgtaag cattggggtt gcacctcgaa
     1441 ttcatgaacc agtttaattt aacgaatctt ttctgtcaaa attaaaataa accttattgt
     1501 ctaattgtag gagtgttaac ctgaaggaac attaaataaa aaaccgctct ctttaattat
     1561 gaaaggggct tgtactttaa ttctaatact cagtttctag ttaaatctac accccttcca
     1621 caaacttcta atcacaataa aaacaggaat aagaagatat agtaggattg ttgttttgca
     1681 gcacacagct attacttgct tttttgtcct ttctcagtgt cactaggtac tagttctctt
     1741 gccttaagtt gagtgatttt ctgtctcagg gcttcgattt taacttttgc aaaggtagtt
     1801 tgaaccgggg ctactacctg tagattatta tagttactaa tggcattttc caaatcccct
     1861 ttctgctcat agcatccggc taaggaaaga taagcttttt ctttcactga atctggtgga
     1921 ggcggagaaa tcttaagaag ttcttcattt gtttttatag aggcttcaca attaccctcg
     1981 ttctgttgtt tgagggctaa atttagttgc tcattatagg cgcttccacg atatgcatat
     2041 agtttcgctt ctttactctt ctccttatcc tctcgaattt cctgttctgc ctgttcctca
     2101 aattgtcctt ctgctagttc ctctctttgt aaagcttcca cttcggtttt aactggcggt
     2161 atggatactg gctcattcat tgatttgtct tccaacctgg ctgaagccat ctcctcagat
     2221 gttttacccg ttacttttaa gtccttctcg acgacaagtc cttctccggg agtaactcgt
     2281 aaagatgtat cagatttttc ttgaattgtt tcctgctttc ctgcagctcg tgaggggatc
     2341 gaatatggtt tactagataa aacactacca ggctttgatt ctataccttc taaaatgttt
     2401 cccctacttg gtggtgcatt tgatcctctt tcgagtggct tggataaaat agcgtgtttt
     2461 tctgatccaa tcttatctaa aaccttattt ccactaacag gaatatttgg ttgctgtgca
     2521 atgggtacct tttttgccat ggcatccctt gaatagatag tttcaccagg agagtaactt
     2581 atgttctttt gtccataata gacccatatc gaaagtgcta gggcagagct cagaaccggg
     2641 actagaatag gtgaataaaa ccacctcttc caaaaggtag acttatcttt tgcaacctcc
     2701 tgcctagctc tctttgataa tgtggctaaa acttgagggg aaggttgagg tatagtttct
     2761 tcattaaata ttcttcgaat ctcttgatat tcctcaagcc ttctgtaaca gcggggacag
     2821 gccgcaagat gcttcttaac cagctccctc tcagaaggtt ctatttcatt gtccataaaa
     2881 tctattaaaa gctcttcaaa cctttcgcaa ttcatattac tctttacacc tctttaaccc
     2941 tttgcctttc tccaaaatat cctgccctaa taaaaacctc tcgaaggttt tggtaagcat
     3001 atctaagacg gctttttacg gtactgagat tagtatcagt tatatcggca atctctttaa
     3061 gtgatagacc ctcgatttct ctaagtaaga atacctctct aaattcttcc cttaattcct
     3121 caattccact atcgagtagt ctttggattt ccttgtctat tatcgcctcc tcctggtttc
     3181 tttccccgct tttaataatg tcgaggacaa ctgcaccatt gatctcgtcc ctagataaag
     3241 aagcatcgag agaatcgtga tgtctatgat tttcggttct aaggtgatct atacagcaat
     3301 tccgtgtaag ggtataaagc catgcagtaa atttttggga tgagtcgtat ttttctttgt
     3361 tctctataac cttgatgaac acctcctgca acaggtcctc ggcttgggta ggggcacgtt
     3421 gcaacctgcc actacttcct atcattttta ttataaatct caacaccccg gtactgtggc
     3481 ggtgtagaag taagtcaaaa gcccgtgcgt cgccgttttg aaacctcacc attgcttcct
     3541 catccgcaag ctcttttttc gcaactaatc caaacactgt gagcgctctc tttcggttct
     3601 ctttgtggcc ttaatataat tatacgatta aaatcagaaa aggttttatc ttctcacaaa
     3661 aattatgtag cacaaacctt ctgtaactcc atatttaact atatatattc tataatattc
     3721 acaaaaaaaa tccctctaat tatttcaaaa tgtaaaatta aagtttataa tgagtcttag
     3781 gattatttaa caagagaggt accatcttga attgggtgtt acttgtataa tatctatcat
     3841 tgttttttgt tataacacat ctccacttat gtcctctgaa aaggattatt cagatactta
     3901 tttgaaaggg aacataatga ataaagatag aaaacctgaa atttatgcgg aatacacaag
     3961 aatccaagaa gagctttgga aagattacga ggagataaga gatgaatcta ttaatggtta
     4021 tcttaggata aagcttcaaa tgtgttacaa agagtttaaa cttttagaaa aatcattaag
     4081 ggataaatac tatagaggga agaagagaat tgcttgagaa gttttttaat aaaatgaaaa
     4141 aaataaaact aactaggggc agacctctgt gcattgacta caacttaagc aaattctggg
     4201 acacatagac gtaggatagg tggggtttta accccgcctg ccaatgcggg actagaaagt
     4261 cccgcatatc cttgctaaat tgacattcgg atgccgctat gatcgcctct catccctcaa
     4321 gttgcaacta atgaaccaca ggggattacc cctacattaa aaaattaaaa agaggaaaac
     4381 catgaaaagc catacagaat atctttggtt taatactaag aacaggaaag aaataatcca
     4441 cattaccgat aaggttaggg aaaccgtgaa gaaaagcgga gtagaggagg gttttgttct
     4501 aatctctgcg atgcatataa cggctgcagt atatgtaaac gatctagaag aggggctttt
     4561 tgaagatatc tgggaatggc tcgaagaact cgctccatat cgaaaggact acaagcatca
     4621 tagaacaggt gaagataatg gagacgccca tcttaaaaat ctaatgatgc atcatcaggt
     4681 gattgtaccg attacgaaag gagatttaga tctggggcca tggcagaggg tcttctatgc
     4741 cgaatttgat ggacagagga gaaaaagggt gattgtaaag gtgatagggg agtgagataa
     4801 atgaaagaac ctgaagaaaa aaaagataaa tttcagccga gaatctatat tgataaagaa
     4861 gggaattggt atcaggatgg gattccaatt ctacacagat ggacttatct ttacaataat
     4921 aaactactaa gacgtgatga ggaaggaaga tattacgtgg atgaaggcag cgggagagtt
     4981 tatgtatatg tggaagatac cccttttgtt gtgaaaatga ttgataaacg acaagaagga
     5041 ttctttataa tcttaaatga tgaaactgaa gaaaagcttg atttcaatgc cctctggttg
     5101 aacaatgaaa acatacctta cacaagggta aaaaatggag aattcgaagc taggttctca
     5161 agaccagcat attacgagtt taccaagtac ttgaagcagg aaggggatag gttttatata
     5221 gaattagatg ggacaaagta tgacttaaat gcaaaaaaat aattaaccac ttaactaaca
     5281 ataaacacga aaacgagtta gcgaacaaga catttctata actcatgtta caaaatattt
     5341 atggttctca tgatgaagct ctctagtgta aaatgtaaaa tgaaagtaca cttattttgt
     5401 taggtcatca tataaatact tccaatccgg attggctttt tcaatgagac cgattttcca
     5461 ttgtcggttc catttcttaa acctcttttc cctttggatg gtgctgtata tgtcattagt
     5521 ctgttcataa tagaccaact tatgtacacc atatttcttt gtaaatcctt caactagatc
     5581 atttctgtgt taataagccc ttctaatgag gtcgttagta ataccaacat ataacgtacc
     5641 gttccttttg cttgccatta tgtaaacgta ataggtatac ataatccccc accactgtca
     5701 tgcccgaatg cattagtcgg gcatccacaa atgttttaat gctggattcc cgctttcgcg
     5761 ggaatgacaa aggaatagtg aactcatggc taagcccgta cccgaatgct cttatcgggt
     5821 aatatgagag aaaaacagcc ttttatttaa cgataaatta gattgtggtt gatccatcaa
     5881 aatattctaa tttattttca cttacgtcca tttataccta ctcttggcgc aattattcca
     5941 aaaataattt tcaatcataa actaaagagt cacaaacttt cataatcagc tcgtttgaaa
     6001 caaggattcc ctttttggtc aggtgaagtg aattatcaga caactctaga aaaccctgct
     6061 tgaatagagc gcttaaacta tcagaataca attttgcccc aaacctgtcc ctcatacttg
     6121 ttagattaat cccctctcca agccttaatc ccatcattat cttatcctga atagcctcct
     6181 ctctactgag aagctcagta aatgctaaag ggattttccc ttccaaaacc gttctcatat
     6241 agagatctgg attttttaag tttgcccacc taacacccca agaagaccct tcatttaaat
     6301 ctagatgtga gtgtgctccc gcaccaagac ctaggtaatt ctcccctctc caataaagaa
     6361 ggttgtgcct gcactcatga ccctgctttg caaagtttga gatttcatat tgcacaaagc
     6421 cggcttgttc aagaaaatcg cttgtaaaaa taagcatctc cgcaagtgtg tcttcatcag
     6481 gaagaggtag cttaccttgt gaataaagcg ctccgaaccc tgtatcatct tcgattgtga
     6541 ggcagtatgc agaaatatga atagtattaa aatctagtgc cttttctaaa tctcccatcc
     6601 actcatcaag tgtttcaaaa gaaaccccga atatgaggtc gaggttaaaa ttattaaacc
     6661 cagctttgat tatgtcctcc aaaaccctcc tgctatcatc aggggtattg atcctcccaa
     6721 gaagtttcag cttcctttct gagaaagatt gtactccgac actcattcta tttaccccaa
     6781 catcctttaa tgcttttagt ttttccaatt cagccgtttt tggattcacc tctaacgaga
     6841 tctctatcga gtctaaagga gtggttacgt ttagaatttt gctaattatt ttgcctatac
     6901 tatccgctga gaataaagat ggcgttccac cgccgaagaa gattgatgta agaggaagat
     6961 tctcgattga ctccctgtag aaatcgagct ctttaagaac tgcctctgtg tattcctttt
     7021 ccggaatacg tctcccaacg ccgtatgagt taaaatcgca atacgggcat ttttttacgc
     7081 agtatggtat gtgaacataa gcgccgaatg tcataaaggt attttacata attaggtaaa
     7141 cccgaacaaa ttaatgaata attattgtaa caacctgatt ttagagtcat atttatactc
     7201 cttataggga ggtattcaga tgagagcact gttacttcta ttaattcttt cttcaagcac
     7261 tcttagcatt ccctctttag cccaggaaat tactgaaaca cttactttag ataaagccct
     7321 tgagattgcg ctagagaaga accccacttt agaagcttcg cgtgcttctg ttgacatttt
     7381 gaaagcaagg gtgcagggtg tgcgctccga cctctttcca cagattgagt ctaggttcat
     7441 atacccattc gttgggacgg aatcaggggt atccttggac cagctcatat gggatttcgg
     7501 ttacacctca aacattatta aggcaaccaa ggcacaagta aaatcctcag aatatgataa
     7561 acaagcaaat cgtgaggatt taatcctaga tattaaagta gcctattaca cagtgctagc
     7621 gcagaagagg attgttgagg catcagaaaa aaaggtcatt gagaatgaaa gacgtcttga
     7681 gcaggctgaa ggttttttta aagtaggtag agtatcaaaa atagagatca caaaggcaaa
     7741 agtaaacctt ggtaatgcta agctcaatct cgtaactgca aagaacaacc tggaaagggc
     7801 aaggttacag cttgcaacag caatggggat aataggggat ttcaactacg aactcgagga
     7861 tatgttagaa tataaaatga tagatttgaa tctagagcag gcaatagata aagcctttga
     7921 gttacgtcca gaactcgata gcctagaagc aaagaaggtt gcgatgaaag cgaatttaaa
     7981 ggcctcaaag caggattatt acccatctat ttttgggaga actgcatata gaatcaaagg
     8041 agaaggagca acacaaccag cctttatagc tggaatcgga gtccaattcc cgatttttga
     8101 aggattttca agcctatctg aggtaaatga ggcaaaggct aatctaaggc gctctcaggc
     8161 agagattcaa tccatgaagg ggcagatact atcagaagta aaacagtcat atcttaacct
     8221 acagctttcg gaagaaagta ttaaagtaac tgagacatcg aaaacatcct cagaggaaaa
     8281 tctcgagctt gctagagaaa gataccgcct tggaagagcc tcttcgattg aacttgcaga
     8341 ggctgaggct ctttatgcct caacaaatgc agactacatt caggctattt acaattataa
     8401 gattgccgtt gctcaactgg aaagagcaag tggggaaaag ctagaagaat gaaaatactt
     8461 gataaaaaaa cagtagttat aattgcaatc ctgctaattg ttgtcgttta tttagcatat
     8521 cgaatctttc aaaacgaagc cacgtcaatc gcttataaaa ccgcaaaggt tgataagggg
     8581 gatataattt cgtacgttac agcaacaggc accttaaatc cgatttccac tatagagatc
     8641 ggcagccagg tttcaggaac tataaaaaac atatttgtgg attttaactc tgtagttaaa
     8701 aagggtgatc ctctttcgga gatagaccca acaccattta attcacaact taaacaggct
     8761 cacgccgatc taaagaaggc agaagctgat gcagagatcg caaagacttt gcaaagggca
     8821 aacgaagtgc tttataaaaa aagattgatt tcaaaagaag aatatgacga ttcaagggca
     8881 aaatactcct cggcgattgc taccttagag cagagaaagg cagcgcttga gattacaaaa
     8941 gcaaatctcg ataacacatc aatccgttca ccaattgacg gaattgtaat ctcaaggaag
     9001 atcaatatta gtcaaatagt tacttccggg cagtcttctt ctcccctttt tctcatagct
     9061 gaagatctaa gtaggatgaa acttgatgca catgtgagtg aagccgacat cggtagggta
     9121 aagaacgaac aagaagccga atttacggtg gacgcatatc ctaaccaaac ttttaaagga
     9181 aaggtatggc agataaaaaa cgaaccaatt accacaaata atgttataac gtatgatgtt
     9241 gtaattctaa ttgataacaa tgaccttaaa ttaaaacccg gcatgacggc agaagtaaaa
     9301 atactcgtgg cacataaaag ggatgtgctt agggttccaa ctgcagcact tagatttatt
     9361 ccaccaccct atgcgcctgt tgatgaagga tttatagaat taaataacac ctcagttgtc
     9421 tgggtacctc taaaaaatgg gcgacttaag gctgtttcta taaacccagg agtaagtgat
     9481 gaggccttta ctgaaatcct agacggaaat cttggtgaag gggaaaaggt aattgtagaa
     9541 gctactgaaa atactgggac tggctccgaa ccgcttggcc ctattgttct ccccaaaccc
     9601 cagaggtttt aaaacagtgg aagatattat agttgtagaa aacctgcata aggtttataa
     9661 gatgggtgat gtggatgtcc atgctttacg aggaataaca cttactataa aaaagggggg
     9721 atttatatct gtaattggac cctctggatc tggtaaaagc actttaatga atataattgg
     9781 ttgcttagac aaacctacaa gtggaagata ccttttagaa gggaatgaca tctcttcact
     9841 agacaacgat gagcttgcag atattagaaa taaaaagatt ggttttgtat ttcaaaattt
     9901 taaccttctt ccaaggtcaa ctgcaattga aaacgtagaa ctaccattaa tctacaatgg
     9961 catcccttct gaaaaaagat atagacttgc taaagaagcg cttttcatgt tggggctaga
    10021 agggagagaa aaacactatc caacccagct ctcaggcggg cagcaacaaa gggtagcaat
    10081 agcaagagct ataataaata aacctagcat catcttagca gatgagccta caggaaatct
    10141 tgatactaat acaagcattg agttaatgga aatttttaag agactaaata aagatggaaa
    10201 aacaattatt atgattacac atgagactga tattgcagcc tacgccgaaa gagttgttat
    10261 gtttagagat ggtaagatca tgaaagaaga attgaggagt aaggaaaagc ttcatgaacc
    10321 cgttagtcgc ctttagggtt tcatacaggg ctttacgaag gaataaagtc cgctcgatac
    10381 ttactacact cggtatcatc atcggtgttg ccgccgtaat aacaatggtg tcacttactc
    10441 agggagcaaa aagaataata gaagaacaat taacaagcct tggtggtaac tcactcataa
    10501 ttaatccagg aagtcgaacg ggaagcggtg taaccaaagg cttaggtaag accaataccc
    10561 ttccttctga ggatgcggag gctatacgaa agctaagcac agttattcac gtatcgccga
    10621 ttctagatac aacagcacag gttgtctggg gcaatcggga ctggtttaca gcacttgtgg
    10681 gtgtttcacc tgacttcgtt tttataaacg gctggtttcc agagagaggt aacttttttc
    10741 acaatgaaga cgttaagaac gcagaacttg tgtgcgtgct aggaaaaacc gtagcgtcaa
    10801 agctttttgg atatcaaaac cctttgggta aaacgattag aataggtaac atctcattta
    10861 aagtaattgg cgttatgagt actataggac aaacacccgg tggaaaggat caagatgatg
    10921 ttgttctaat cccttacact acactgcaga aaagaatatt gggtgttaac catgtggata
    10981 acatatccgt atccgtcaga tcatcaaaag atataccatt tgctgaggct caaataacag
    11041 agcttttgag ggaacgtcac cgtataagac caaacatgga agatgacttc tacgtaaaaa
    11101 cacaacttaa catcatagaa agaatcttca caatttcaaa gataatgacg attctactcg
    11161 gaagtatcgc ctccatttca ctaatagtag gtgggatagg aatcatgaac ataatgcttg
    11221 tttctgtcac agagagaaca agagaaatag ggataagaat ggctgttggc gccaaggagc
    11281 gagatatact tattcagttt ctcatagaag ccgttgtact ctcactaaca ggcggagtta
    11341 tagggatcgt attaggtata gtaggatcaa agatttcctc gttttttacc cactggccga
    11401 ctcttgtttc ttttggctcg atattactcg cttttggttt cgcagcgatt gtagggattt
    11461 tcttcggtct ttatcctgct agtaaagcat caaaattaga cccgattgaa gcactcagat
    11521 acgaataata gttaaaactc cgctctaaca aaaccaagcc cactggcagc aacatcgttt
    11581 ataaagtcac ccgatatatc accgacacct agaactatac ttccaaattt atcatcagca
    11641 agaaaacctt gactcattcc attcaagggg ggaaggcata acgaggcttc catcgcgcat
    11701 gagcttgcaa gaactgaagc cggaaaacca tcgtcacttc caaaaaaaac ataaaccgac
    11761 cctccttcgg ctgttccagg tgcacctatt gcaaaatcag gatctgtagg aata
//
LOCUS       DATDRL010000131         6350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2001757, whole genome shotgun sequence.
ACCESSION   DATDRL010000131 DATDRL010000000
VERSION     DATDRL010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001757"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VI935_04760"
     CDS             <1..865
                     /locus_tag="VI935_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLE24945.1"
                     /translation="IVRTSGKAKLRYQTHRAVDGAYEVITATEVTPGDVNEAQKMLWL
                     IDTHQLNTETNVETVVADSKYGTISNYLDCYDRGIRAHIPDLKEAQSNSGFRGKIFSD
                     EMFIYDGKTDTYTCPSGKGLRRKSLHMSRRSMDYAALGSDCRICSLRPHCTKNKAGRT
                     VKRHLRQEELDNMRALAGTALSKNDIKTRQHLMERSFARAKRYGYDRARWRGLWRVRI
                     QEYLTAAIQNIQILLKYGTDPRIAVGVAKVDGELNGKITGVFKNINKSLLEFFLLFKS
                     EVEPYLVLKTS"
     gene            complement(1108..1608)
                     /locus_tag="VI935_04765"
     CDS             complement(1108..1608)
                     /locus_tag="VI935_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLE24946.1"
                     /translation="MNTQTHKPAVLQSNEGRKMTVLGMEVTLKLASTETAGDYYLFEI
                     VVPPGLGVPPHFHEREDEILKVMDGEFEVFLDGKTYKAASGAVAYFPRNVVHGFSNTG
                     KTLGRAMFFVSPARILKNSLKNWAHCQPINHQTWPKLGRFLTAMRSELSPNRRLRHKW
                     GANCAA"
     gene            complement(1764..2189)
                     /locus_tag="VI935_04770"
     CDS             complement(1764..2189)
                     /locus_tag="VI935_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24947.1"
                     /translation="MNAPSTNSIRWPKWTLLTVFLLTVPVPYYMVVVGGMVPTFFIIF
                     IAVQGLIVALPKFTAEGFWMLAILWAHVVILGGLLYLVAHGITWLLLRILPYRYALLV
                     VIGLIATLFIASTFEIYRMPGHNSSPPANILHIFKSLAT"
     gene            3041..4018
                     /locus_tag="VI935_04775"
     CDS             3041..4018
                     /locus_tag="VI935_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLE24948.1"
                     /translation="MGVFRRTIKGKDGKKEYWYIDYVLDAKRKWESVGQVGRVSKEHA
                     KKLLALRKTEILQGKFNAPKPKTIPTLSEFAKEYLEFEKGNKKSWDRDMYAVIRLMPF
                     FGSYRLKDISPILIEKYKLERKQGVSNRTVNIELSLLRRMLNLAVSWDKCESNPVSKV
                     KFFKEAPPKERILTLEEEKALLESSPSHLKPVLITALNTGMRYGELLNLTWNDVDFDS
                     GYIHVRQSKSGRSRKKPMNETVRETLLELESENFVSNSVSSGFDLAKKTKDYRGVLPL
                     THVPVKPEVSGSSPVGPVFKKSELTGIFSIQPFRLLHKKSSSHPYAGSL"
     gene            complement(3939..5135)
                     /locus_tag="VI935_04780"
     CDS             complement(3939..5135)
                     /locus_tag="VI935_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HLE24949.1"
                     /translation="MILRSITLKNFRSYKSEIFTFHERFNLVSGENAQGKTNLLEAIH
                     LLCTLRPFKQVRMEELISFGETEGRIKGELESDSGLNEVHIILTSGSPTTLPLNRYPS
                     SRGSPTTLPLDPRSSRGYPRPQGGKTVKLNGKIVYDIPKLLGRFNVVTFLPSDLDLIK
                     GSPQDRRRYIDALICNFKSEHLIDLKLYLRALTQRNALFLREPTRETLDAWDEKIGEL
                     GGKIVKRRIKLIERMVPLLERNYRLISGLNSRIKIQYKPSFNLGSDVAKGLKNELRAR
                     LYLDKKRRHTSVGPHRDIPGFTMDGIDASVFASQGEAKTLALALKASEIELTRLTLGR
                     NPILLLDDITSELDERRKNFLYRLIEEYSGQIFVTATNPKEIQHKGEKKIFYVKAGRA
                     ESKIYQ"
     gene            5403..>6350
                     /locus_tag="VI935_04785"
     CDS             5403..>6350
                     /locus_tag="VI935_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLE24950.1"
                     /translation="MLKIAEISVKDAGKGLGRFDPKDMHALGIAAFDLIEIGGKRRTV
                     VRAMPLEKPQRGQSLIQVDGITRENARVGIDDSVFVKKVESKQAIKVVLAPISDDLLL
                     NGDNTDYLVSRLDGIPVTVGDRVRITLPGSRIEDFQVLGAIPAESIVVRTTTKIELRK
                     KPKGKVDTSRVTYEDIGGLKEQIRKVREMVELPLRYPQVFERLVIDPPRGVLLLGPPG
                     SGKTLLAKAVAHETNASFQAINGPEIIHKFYGESEARLRDIFEIAERNQPSIIFLDEI
                     DAIAPKREKVSGEVEKRVVAQLLALMDGLKDRGRVIVVGA"
BASE COUNT         1872 a         1194 c         1481 g         1803 t
ORIGIN      
        1 catagtaagg acaagtggga aggcaaagct taggtatcaa acacataggg cagtggacgg
       61 agcctatgag gtaataacag caacagaggt aacgcctggg gatgtcaatg aagcacagaa
      121 gatgctttgg ttaatagata cacatcaact aaatacagag acaaacgtag agacagtggt
      181 agcggatagc aagtatggga caattagtaa ttacttggat tgttacgaca gaggaattag
      241 agctcatata cctgatttga aggaagcaca gagtaatagt ggttttaggg gaaagatatt
      301 ttcagatgag atgttcatct atgatgggaa gacagatacc tatacatgtc cttcaggaaa
      361 gggacttagg cgcaagtcat tacatatgag tagacggagc atggattatg cagctttagg
      421 gagtgattgt aggatttgta gcttgagacc acattgtact aagaacaaag caggtaggac
      481 ggtgaagaga catctgaggc aagaggaact tgataatatg agagcattag cggggacagc
      541 actatctaag aacgacataa agacgaggca gcatttgatg gagaggtcat ttgccagagc
      601 aaagaggtac ggatatgata gggcaaggtg gaggggatta tggagggtga ggattcagga
      661 gtaccttact gcagcgattc agaatataca gatactttta aaatatggaa ctgatccgag
      721 aatcgcagta ggagttgcta aggtagatgg ggaattaaat gggaagataa caggagtttt
      781 taaaaatatt aataaatcat tattagaatt tttcttatta tttaaatctg aggtagaacc
      841 ctacttggtg ttgaaaacca gttgattgat taaaacaaaa ttcatttgag caacagcccg
      901 tcaacatttg acagaagcaa gttttatgcg aatagaatgg agtctcgtct taaattatga
      961 atgtttattt cactaactaa tttttgaggt tgcttcttga ctctgcactt tttaagattc
     1021 aagtcaatac ggatgcctga ccctatttgt ttcgtcagcc tggcgggcaa ggctggcgca
     1081 cgtaggcggg tatgctccat cgagttgtta ggcggcacaa tttgcacccc acttgtgcct
     1141 aagccgccgg tttggagaca attcggatcg catagcggtc aaaaatctcc ccaactttgg
     1201 ccatgtctgg tggttgattg gctggcaatg cgcccaattc ttcaaagaat ttttcaaaat
     1261 tcgcgccggg ctaacaaaaa acatggcccg gccaagggtc ttgccagtgt tactgaatcc
     1321 gtgaacaaca ttccggggga aataggccac cgcaccagat gctgccttgt atgtttttcc
     1381 gtccaaaaag acttcaaact ctccgtccat gactttgagg atttcatcct cacgttcgtg
     1441 gaagtgcgga ggaacgccga gtcctggcgg aactacaatc tcgaagagat aataatcgcc
     1501 cgccgtttcg gtgctggcca gcttcagtgt gacctccatg ccaagcaccg tcattttccg
     1561 cccttcattc gattgcaaga ccgctggttt gtgcgtttgg gtgttcatgc tcatttccta
     1621 caagtgagtg ttgttggttg tcgtgccgcc taacgtttag ggtcagcggc caggccgaca
     1681 acccttacac tatactggtg ccttaactcc gacatatgat tttcgttaac ctggcgattg
     1741 cctggtccga ctgcacccta ttgttaggtg gccaagctct taaagatgtg gagaatattt
     1801 gccggtggag agctgttgtg cccaggcata cgatatattt caaaggttga tgctataaat
     1861 aaagttgcga tcaagccaat cacaaccaaa agagcgtaac gataaggaag gatgcgtaac
     1921 aggagccagg taatgccatg cgctacaagg taaagaagac ctccgaggat cacaacgtgt
     1981 gcccaaagaa tggcgagcat ccagaatcct tcagctgtga attttggtaa ggcaacaatg
     2041 agaccttgaa ccgcgataaa gataatgaag aatgttggca ccataccacc cacaaccacc
     2101 atatagtaag gaactggcac cgttaaaagg aagacggtca atagagtcca tttcggccag
     2161 cgaatggaat tggtagatgg agcattcatg agtgaatctt cgaaatgctt agcgtagaca
     2221 ctttctgctt cctggggcac ctaattattg attatacgga attttttccg tataaatctc
     2281 ctattggggg tgttatgcgg aattctttgt gacatccctc atcatctggg aaacataaac
     2341 ccaatttgtg cataaaacac cctttttttt tcattatctc caaatggtaa gacgttgtca
     2401 agagaaattt tccctcattg tctattccct tggaaagaaa ttgttttttg ccttcaacca
     2461 tcctctaaaa ttcaactagt tcctattctt ttcttgttac tagggcggaa cttgcataat
     2521 tacaaaaggc taggcccaga ttataacaat atcacctaat gttaaatacc aggagatgtt
     2581 tttgaagtta cagaggggag ccagagagaa taatcggggt ctactgtaac tccacaattc
     2641 ctccctttaa tgccgattct tccctgattt cattatccga ctttaactgt gtggtactgc
     2701 caagtttttt gagcttcttc tccccagcaa tggtaaagat ttttggaaac tggatttttg
     2761 tgagagggtg atatatctac atcctgcgtt caattgtccc attcgcccac ttcgagtcct
     2821 catctccatg tgccccatgg ctgcctttgt gctgggggtt ctagggtatc ggggtattga
     2881 atccgacttt ggatatatat atgagagtgg ataagtcctg agcttaccct gaaccctgaa
     2941 acaagttcag ggggactacc tcagtgtaaa ccctgcttta catcacgaag caggagttgt
     3001 attatcggca agagatggat ctaaatgaag ggggtaacaa atgggagtat ttagaagaac
     3061 cattaaaggc aaagatggta agaaggagta ttggtatata gactatgttc ttgatgccaa
     3121 gagaaaatgg gaaagtgtag ggcaggtagg aagagtcagc aaagaacatg ctaaaaagct
     3181 cctagcactt agaaagacag aaatactaca gggcaagttt aatgcaccaa agcctaaaac
     3241 aattcctacc ctctcagagt ttgctaaaga gtatcttgaa tttgaaaagg gtaataagaa
     3301 gtcatgggat agggacatgt atgcagtaat aaggttaatg cctttttttg gctcttatag
     3361 gctaaaagat atttctccta tcctgattga gaaatacaag ttggaaagaa aacagggtgt
     3421 ttcaaacagg acagtcaata tcgagctttc actgcttaga aggatgctta acttggctgt
     3481 ctcctgggat aagtgtgaat caaacccagt aagcaaagtt aagtttttta aggaagcacc
     3541 acctaaagaa agaatactca ccctagaaga agaaaaggca ctactagaat cgagccctag
     3601 ccacctaaag cccgttctta ttacggcact taatacaggc atgagatacg gagagcttct
     3661 taacctgact tggaatgatg ttgactttga ctccggctac attcacgtaa gacaaagcaa
     3721 gtcaggaagg tctagaaaga aaccaatgaa tgaaactgtc agggaaacac tactagagct
     3781 agaatcagaa aattttgtta gcaattctgt tagcagtggg tttgatctag ctaaaaaaac
     3841 taaggattat cggggagttc ttccactgac acatgtgcct gtcaagccag aggtctcggg
     3901 ttcgagtccc gtcggccccg tttttaaaaa atctgaactt actggtatat tttcgattca
     3961 gcccttccgg cttttacata aaaaatcttc ttctcaccct tatgctggat ctctttagga
     4021 ttggttgctg taacaaaaat ctgccctgaa tattcttcaa taagtctata gagaaaattt
     4081 tttcgtctct catcaagctc actcgttata tcatctaaaa gaaggattgg atttctccca
     4141 agggttagtc ttgtgagctc gatctcagat gctttcagtg cgagtgcaag ggtttttgcc
     4201 tcaccctgag aagcaaatac cgatgcatct attccgtcca ttgtgaatcc tggaatgtct
     4261 ctatgagggc cgaccgatgt atgacgtcgt tttttatcaa ggtaaagcct tgcccttaac
     4321 tcatttttta accctttcgc aacatcactt cctagattga aagagggttt atactgaatt
     4381 tttatccttg aatttaaacc tgagataagc cgatagtttc tttcaagaag tggcaccatt
     4441 ctctctatta atttgattct tcttttgact atcttccctc caagctctcc tattttttca
     4501 tcccatgcat cgagtgtttc tctagtcggt tctcttagaa aaagggcatt tctctgggtt
     4561 agcgctcgta aatataattt taaatctata agatgctcgg acttgaaatt acatataagt
     4621 gcatcaatat atcttctcct atcttgaggt gaacccttta ttagatctaa atctgatgga
     4681 aggaatgtca cgacattgaa tcttcccaga agcttaggta tatcgtagac aatctttccg
     4741 ttaagcttta cggtttttcc accttggggt ctggggtatc cccgactgga tcggggatct
     4801 agagggaggg tagttgggga accgcgggaa ctggggtagc gattgagagg gagggtagtt
     4861 ggggaaccgc ttgtaagaat tatgtggacc tcattaagtc ctgaatctga ttcaagctca
     4921 ccctttattc tcccttctgt ttctccgaaa ctgataagct cttccattct tacctgtttg
     4981 aatggtctaa gtgtgcagag taggtgaatt gcttcaagca ggttagtttt tccctgtgcg
     5041 ttctctccag agactaaatt aaacctttca tggaaggtaa agatttcgga tttatagctt
     5101 ctgaagtttt taagggtgat tgatctaagt atcaacggag tgcagctctg gtgtttgaaa
     5161 aaagtataat aagaatcatg tgcttattca atattgctaa tttgctgcat caaattccaa
     5221 ttctcaaatt ccaaaatcca aacaaatcca aaatccgaaa ttctaatgac caaaagtttg
     5281 gaatttgtga tttggttatc gaaatttgtt tgtgatttag tgcttgggat ttggagcttg
     5341 tttattttgc ttgattttaa atagcgatag cggtaatatt ttaattagtg aatgacatga
     5401 taatgcttaa aattgctgag atctcggtaa aggatgcagg aaagggcctt ggaaggtttg
     5461 acccaaaaga tatgcatgcc cttggtatag ctgcttttga tttgattgaa ataggaggaa
     5521 aaagaagaac tgtcgttagg gcaatgcctt tagaaaagcc acaacgtggt caatccttga
     5581 tccaggtgga tgggattact agagagaatg ccagggttgg aattgatgat agtgtgtttg
     5641 taaaaaaggt cgaaagtaaa caggcaataa aagtagtgct tgcgcccata agcgatgatc
     5701 ttttattaaa cggtgataat acagattact tagtgagccg tcttgatggg atcccagtta
     5761 cagttgggga tagggtaaga atcaccctcc ctggttcaag aatcgaggat tttcaggtgc
     5821 ttggggctat ccctgcagaa tctattgttg ttcgtactac aacaaagata gagcttagaa
     5881 aaaaaccaaa aggtaaggtt gacacctcaa gggtaaccta cgaggatata ggtggtctaa
     5941 aggaacagat tagaaaggta agagagatgg tagagcttcc tctcagatac cctcaggtat
     6001 ttgaaagact cgtgatagat ccaccaagag gggttcttct tttaggacct cccgggagtg
     6061 gaaagaccct acttgcaaag gcagttgcac atgaaaccaa tgcatctttt caggcgataa
     6121 acggtcctga gataatacat aagttctatg gcgaaagtga agctaggctt agagatattt
     6181 ttgaaatcgc tgagagaaac caaccctcta ttatctttct tgatgagatt gatgcaattg
     6241 caccgaaaag agaaaaggtt tctggggagg tggaaaaaag ggttgtggcc cagcttcttg
     6301 cacttatgga tgggctgaag gatagaggta gggtaatagt agttggggcg
//
LOCUS       DATDRL010000132         2616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2124499, whole genome shotgun sequence.
ACCESSION   DATDRL010000132 DATDRL010000000
VERSION     DATDRL010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2124499"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1235)
                     /locus_tag="VI935_04790"
     CDS             complement(123..1235)
                     /locus_tag="VI935_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLE24951.1"
                     /translation="MTQYANKEGTARYRDRSRDTLPIDHFRESQGLWMSSIGFGSYLG
                     NYDESTDIRYRDAVVRAIELGCNKIDTAINYRFQRSERSIGAALQILFNDGKASRDEL
                     IIATKGGYIPFDGAPPRDMNTYFIETFIKPGIATPEDIVSGSHCMTPRYIENQLECSL
                     RNLGLECIDIYYLHNPEEQLQEVSRQEFNKRISAAFEMLEKQVSQGKIRMYGTATWNG
                     YRQEQNAPDCLSLEELVGIAKEVSGEDHHFKVIQLPFNLAMPEALIIKNQRVDGDMTS
                     ILEASWRLGVTVIGSSSLLQSQLSKNLPDFIGECLKGLETDAQRAIQFARSTPGIASA
                     LVGMSRVDHIEENMKVAKISPASIEDFVKLFASEEE"
     gene            complement(1573..1773)
                     /locus_tag="VI935_04795"
     CDS             complement(1573..1773)
                     /locus_tag="VI935_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HLE24952.1"
                     /translation="MRKGKVKWFNESKGFGFIEQEGGKDVFVHYSAIQNSGFKTLTEG
                     DVVEFDVVEGPKGLQASKVNKV"
     gene            2058..2576
                     /gene="tpx"
                     /locus_tag="VI935_04800"
     CDS             2058..2576
                     /gene="tpx"
                     /locus_tag="VI935_04800"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HLE24953.1"
                     /translation="MAQERTGAITMRGNPVTLVGPEIKVGDKAPNFTSLEALGSPITL
                     DSLKGKVKVFNVIVSVDTPVCDAQTRRFNEEAAKLPGDVAILTLSMDLPFAQKRYCGA
                     AGIDKVKTASDYRDASFGHAYGILIKEHRLLGRAIFVIDKNDTVQYVEYVKEVTQHPD
                     YDAALAVVKKLA"
BASE COUNT          756 a          542 c          514 g          804 t
ORIGIN      
        1 taaatctttc agctacatac aaatgatata actttagcgg aatcctttag gattccattg
       61 gaatataaat atttagctgt cgtttcattt gtgttcatta gtgtaaattc gtggctaaaa
      121 tcttattctt cttcacttgc aaatagcttt acaaaatctt ctatcgaggc gggtgagatt
      181 ttggcaacct tcatgttttc ttctatgtgg tctaccctgc tcattccaac tagagccgat
      241 gcgatcccag gtgttgaacg ggcgaactga atggcgcgct gtgcatcagt ttctaatccc
      301 tttaagcatt caccaataaa gtcagggagg ttttttgaaa gctgactttg gagaagggat
      361 gagcttccga ttacagtaac tcctagcctc catgaagcct caagaatgga ggtcatatcc
      421 ccatctacac gttggttttt aatgattagg gcctcaggca tagctagatt aaagggaagc
      481 tgtattacct taaaatggtg atcttcacct gaaacctctt ttgcaatacc tacaagctcc
      541 tcaagtgaca aacaatcagg ggcattttgt tcttgacggt acccgttcca ggtagcagta
      601 ccatacattc ttatctttcc ttgagaaacc tgcttctcaa gcatctcaaa ggcagcactt
      661 atacgtttat taaactcctg tcttgatacc tcttgaagct gctcctctgg attatgaagg
      721 tagtaaatgt caatgcactc aagaccaagg tttctaaggc tgcactcaag ttgattttca
      781 atgtaacgag gagtcataca gtgactgcct gatactatat cttcaggagt agcgattcct
      841 ggttttataa atgtttcaat aaaatacgtg ttcatatccc ttggaggagc accatcaaaa
      901 gggatgtagc caccctttgt agcgatgatt agctcatcac gtgaagcctt tccatcgtta
      961 aataagatct gaagagctgc accaattgac ctttcactac gttgaaacct gtagttaata
     1021 gcagtgtcaa ttttattaca tccaagttca atagcacgga ctacggcatc tcgatacctg
     1081 atatctgtag attcatcata gtttccgagg taagagccga atccgataga ggacatccat
     1141 aaaccctgag attctctgaa atggtcaata ggaagtgtat ccctagacct atcacgatat
     1201 cgtgcggtac cttccttatt tgcatattgt gtcatgatgc cgcctccgtt ttcttttcag
     1261 tattctacct gaatgttggc ttgattgctg aaaaaaagct actgctctgc atggacttta
     1321 ctaaaactct ttgaggtacg gagggtactt ggggaaacca actttaccta tgacaattct
     1381 acgaaaccgc caactcgaga tagatttctc tcatttgcgg atagggctat caatccgcac
     1441 aatattatag attaagagca aagcaacatt ttgagttacc ttactaatct atttaaaatt
     1501 cactccctgg attttacttt tcttcgaatt gttcttagaa acttctcagg gaaggaacta
     1561 aatgtgaagt tatcaaacct tattaacttt gcttgcctga agtcccttag ggccttccac
     1621 gacatcgaac tccacaacgt caccctctgt aagggtttta aaaccagaat tctgtatagc
     1681 actgtaatga acaaaaacgt ccttaccgcc ttcctgctca atgaatccga aacctttgga
     1741 ctcattaaac cactttactt tacctttacg caatttactg cctccttaaa attaataact
     1801 taaatatcac atgcttttat tgccttgtca aatcttatct attagacgca aataggcatg
     1861 aatgataata ggcgagatgc gggatacaag atgcaagatg caagatgcaa gatataagat
     1921 tatgaatcat gcatcgtaaa tcattttatt atcttaaatt cgtggctaaa taatcaccac
     1981 ttcctattga caccaaaggg tcttatatat agtctaatca attaattaat aatatatctt
     2041 gaggaggtac gacaaaaatg gcgcaggaaa gaaccggtgc aattactatg agagggaatc
     2101 ctgttacact agtaggccct gagattaaag ttggcgataa agcccctaac tttacttctc
     2161 ttgaagcctt gggatcacca attactcttg atagcttgaa gggaaaggtt aaggttttta
     2221 atgtgattgt atcagtggat acccctgtct gcgatgctca gacaaggcgc tttaacgagg
     2281 aggctgctaa gcttccaggc gatgtggcga ttcttacttt aagtatggac cttccgtttg
     2341 ctcagaagcg ctattgcgga gctgctggaa ttgacaaggt taaaactgca tctgattatc
     2401 gtgatgcctc cttcggtcat gcctatggca ttttgataaa ggaacaccgt cttctaggca
     2461 gggctatatt tgtgatagat aaaaacgata cggtacagta cgttgaatac gttaaagaag
     2521 tgactcaaca tccagactat gatgcagctc tcgctgttgt taagaaatta gcttaggtga
     2581 tgatttagta gagccctgag ttaatgggat tgggct
//
LOCUS       DATDRL010000133         6379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3170910, whole genome shotgun sequence.
ACCESSION   DATDRL010000133 DATDRL010000000
VERSION     DATDRL010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3170910"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VI935_04805"
     CDS             complement(<1..596)
                     /locus_tag="VI935_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLE24954.1"
                     /translation="MDTKKPKMRVSEILPNITYRCRWVWYRNLIVWTKFYKSSIVANL
                     GEPVLYLLALGYGFSPLISDVNGMSYMQFLAPGLISYTAMNAATYECTFSAYARMTAQ
                     KTYDAIISTPVDIDEVVAGEVLFGTTKAFFASLAMMVVLSLFKLIPAPTALLVPFVVL
                     VTGFVFASLAMLFTSFSQSWDFFSYYFTLLIAPLYFLSG"
     gene            965..2266
                     /locus_tag="VI935_04810"
     CDS             965..2266
                     /locus_tag="VI935_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HLE24955.1"
                     /translation="MPLIALLLGTGVYLTIILKGLQFRALIPSLYIALIKRKEERESE
                     GDISHFQALMTALAATVGVGNIAGVATAIAVGGPGAMFWMWLTGLFGMATKYSEAVLA
                     IKYREVDKFGTMSGGPMYYISKGLGLRWLGTLFAIFASIAAFGIGNMVQSNSVADALN
                     STFGVSHWVSGVVMCIATGLVVIGGIKSIAKAATVIVPFMIVFYMIGSFISIFVHWQN
                     VPEAFRLIFYHAFTPTAAAGGFMGAAVSHTIRMGVARGIFSNESGLGSSPIAAAAAQT
                     RNPVRQAMVSMTQTFIDTIVVCSFTGIVIIASGVWASGKTGASLSTFAFEQGIPTRIG
                     DNIVAISLAAFAYSTILGWCYYGEKAIEYLFKERAVKPYRILFTVVVLAGAVVKLELV
                     WTFADIMNGLMAFPNLVGLLGLSGVVVEETKKYLELEISGK"
     gene            2394..2645
                     /locus_tag="VI935_04815"
     CDS             2394..2645
                     /locus_tag="VI935_04815"
                     /inference="COORDINATES: protein motif:HMM:NF044561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLE24956.1"
                     /translation="MSVTKSNSSVSIDRYVDLALKHAEDIQNDDGTWTVKIPVLPGLI
                     TFGETKEEAKGMAEDAVRGWIEIAKQFGDEIPEINGCVL"
     gene            complement(2670..2894)
                     /locus_tag="VI935_04820"
     CDS             complement(2670..2894)
                     /locus_tag="VI935_04820"
                     /inference="COORDINATES: protein motif:HMM:NF044474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24957.1"
                     /translation="MKQKVGTLLDEKVIIALKVIAAKEKRRLSDVIEEALIQYMQRKH
                     GKTVQRTKGAIPASPEVVKAILEEEPFCET"
     gene            complement(2981..3244)
                     /locus_tag="VI935_04825"
     CDS             complement(2981..3244)
                     /locus_tag="VI935_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002078791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLE24958.1"
                     /translation="MESVRAKQFKVIVEKHPDGYIAYPLGLKGIVVGEGDTYEEALAG
                     LKSAIRFHIETFGRDVLEEGLSIPEAFVAKREYPYSPNKANLE"
     gene            3658..4050
                     /locus_tag="VI935_04830"
     CDS             3658..4050
                     /locus_tag="VI935_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HLE24959.1"
                     /translation="MRNLTYLIMSVFLATFALIFATETAIAQDAVKADPNHYKVEFEN
                     DQVRVLRISYGPHEKSVMHEHPDSVAIFLTDQYVKFTSPDGKSEEMRTKAGDTRWLPG
                     GKHLPENLSDQPLELILVELKTMPAMTK"
     gene            4287..5105
                     /locus_tag="VI935_04835"
     CDS             4287..5105
                     /locus_tag="VI935_04835"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLE24960.1"
                     /translation="MENLSQLNIPFLKEPRAVDALESKKLKWNVSLIESRSILSKTGI
                     SCFDYVINCYRGCSFGCLHCYASFMKKFTGHKEKWGDFVDVKVNSVELLKKEIKKINK
                     GRVFLSSVTDPYLPIEAKFKLTRGVLQCFIDTDFEINILTRSPLVTRDIDIFKKLKSV
                     EVGLSIPTDREDVRKIFEPNATPISSRIKALGKLKEEGINTYAFIAPLLPLNPENLAK
                     LLNPIADYVMLDGLNYSWKVSDIIRKHRFEYILDPKWIREVEEKFQRLLGGKFS"
     gene            5151..5372
                     /locus_tag="VI935_04840"
     CDS             5151..5372
                     /locus_tag="VI935_04840"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HLE24961.1"
                     /translation="MYEAKLSSKNQIVIPRDARNALRIKAGDRLLVVVRGDTVILLRK
                     PKNYSKAISETAKGIYSSDYLEKERESWR"
     gene            5369..5827
                     /locus_tag="VI935_04845"
     CDS             5369..5827
                     /locus_tag="VI935_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HLE24962.1"
                     /translation="MRRKRLEDFFEQHRKIGLDTSVFIFEIEGNPRYIKLVNPIFVRL
                     DGPKGRAVTSTITMLELLVQPYRLSDIDSVNKFYALLSTYPHLEWIEPTLEIADLAAR
                     LRAECNLKTPDALQAATALAHQATGFISNDTAFKRVGGLEVLILDELLFV"
     gene            6083..6167
                     /locus_tag="VI935_04850"
     tRNA            6083..6167
                     /locus_tag="VI935_04850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6117..6119,aa:Leu,seq:caa)
     gene            complement(6181..>6379)
                     /locus_tag="VI935_04855"
     CDS             complement(6181..>6379)
                     /locus_tag="VI935_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLE24963.1"
                     /translation="IPLGDLPSRVNELDTADEIIVHCHHGVRSLRATTFLRNMGFKKV
                     KSLKGGIDVWAQEYDQDMPRY"
BASE COUNT         1879 a         1208 c         1407 g         1885 t
ORIGIN      
        1 ccagagagaa aataaagggg agcaatgaga agcgtaaaat agtaactgaa gaaatcccag
       61 gattgagaaa atgatgtgaa aagcattgcg agagatgcaa aaacaaaccc tgtgacaaga
      121 actacaaatg gaacaagtaa ggcggtggga gcaggtatga gtttaaaaag agaaaggacc
      181 accatcatag ctaggcttgc aaagaatgcc tttgttgttc caaaaagaac ctcaccggca
      241 actacttcgt ctatatcaac aggggttgaa attatagcat cataggtctt ttgagcggtc
      301 attctggcat aagcgctaaa ggtacactcg tatgttgctg cattcatagc tgtataggaa
      361 attagcccgg gtgctagaaa ctgcatgtag ctcattccgt ttacatctga aatcaaagga
      421 gaaaatccgt agccaagggc aaggagataa aggactggct ctccaaggtt tgcaacgata
      481 cttgatttat aaaacttggt ccagacgatt aaatttctat accagaccca acggcatctg
      541 taagttatat ttggtaaaat ctctgatact ctcatcttgg gtttttttgt atccatactg
      601 tgctgcaaaa caatgcatta ttcatgcgat attgtagggg cgaatcttgc attcgcccaa
      661 aatagtgggc aatcgcaagg attgccccta ccaattacaa ctatggaaag tttaccggat
      721 aactataaat cgtttttttg accatctcaa atttctatgc gttctgcttc aaagagaaat
      781 ttgttgccac caatacacta aagcactaag aaaacttcgt gtcttcgtga ccttgtagca
      841 aattatgcat tcctgcacag acatgataag gtataaaccc aaaatgaaaa tctaatggag
      901 gcaagatccc atacaagaag aaattataaa gatactctct gatataagta accttgtctg
      961 ggggttgccc ctgatagcgc ttctcctagg aaccggtgta tatcttacga taattttgaa
     1021 aggactccag ttccgcgcgc ttattccctc tttatatatt gccctaataa aaagaaagga
     1081 agaaagggag tccgagggtg atatttcaca ttttcaagca ctaatgactg cacttgctgc
     1141 aacagttggt gtaggaaaca tcgcaggagt tgcaactgca attgcagtag gtggccccgg
     1201 agcgatgttc tggatgtggc ttacagggct tttcggtatg gcgacaaagt actcagaagc
     1261 agtgcttgct atcaaataca gagaagtaga caaatttggc actatgagcg gcggtcccat
     1321 gtactacatc tcaaaggggc ttggactgag atggctcggt actctttttg ccatatttgc
     1381 atccattgcc gcctttggaa tagggaacat ggttcaatca aactcagtgg cagatgcgct
     1441 taattccact tttggtgttt ctcactgggt ttcaggggtt gtgatgtgta tcgcaacagg
     1501 gcttgtagta attggtggaa ttaaaagcat tgcaaaggca gcaactgtaa tagttccatt
     1561 tatgatagtt ttttatatga ttggttcttt tatttcaatt tttgttcact ggcaaaatgt
     1621 tccggaggca tttcgtctaa ttttctatca cgcctttacc cctactgctg ccgctggtgg
     1681 gtttatggga gcggctgtaa gtcacacgat aagaatgggg gttgcacggg ggatattctc
     1741 aaatgagtca ggtcttggga gttctcctat tgctgccgct gccgcacaaa caagaaaccc
     1801 tgtaagacag gcaatggttt ctatgaccca gacatttata gatacaattg ttgtttgcag
     1861 tttcactggg atagttataa tcgcaagtgg cgtctgggct agcggaaaaa ctggggcatc
     1921 tctttcaaca tttgcttttg agcaagggat tcccacgcga attggtgaca acattgtggc
     1981 aataagccta gccgcattcg cttattctac gattcttgga tggtgttact atggggagaa
     2041 ggctatagag tatcttttta aagagcgcgc agtaaaaccc tacagaattc tttttacggt
     2101 agtggttctg gcgggcgctg ttgtgaagtt ggaattagtc tggacttttg cagacataat
     2161 gaacgggctt atggcttttc caaaccttgt cggactcctc ggactttcag gggttgtagt
     2221 ggaagagaca aagaagtatc tggagcttga gatttcgggg aagtagtttt aacaaatttc
     2281 cgaataaaat gaacagatcc tatttggtta ttacaaataa gagaacttat gcacttgaca
     2341 tgaaataatt accgatgttc gttcatctaa taaaatatat aggagatttt tagatgagtg
     2401 tgaccaaatc aaactcgagc gtaagtattg ataggtatgt agatttagcg ttaaaacatg
     2461 ctgaggatat tcagaatgat gatggaacgt ggacggttaa aattcccgtt ctacctggtc
     2521 ttattacatt tggagagaca aaagaagaag ctaaaggaat ggcagaagat gcagttagag
     2581 gatggataga gatcgccaaa cagtttggag atgaaattcc agaaataaat ggatgtgttc
     2641 tatagacttt agcaagggcc ttaatctcac taagtttcac agaatggttc ttcctctaaa
     2701 atagccttta ctacctcagg agatgctgga attgctcctt tggttcgttg aaccgttttc
     2761 ccatgctttc tctgcatgta ttgtatcaga gcttcttcaa taacatctga tagtcttctt
     2821 ttttccttcg cagcgattac cttaagcgct ataataacct tttcatctaa gagagttcca
     2881 accttttgtt tcattttcgt attctctttt ttcacttaat ttaaaaggag gataatagat
     2941 tttagtaaga aatcaagata aaaatctagt tttctataaa ctattccagg tttgccttat
     3001 ttggggaata aggatattct cgcttggcta caaaggcttc tggtatagat aatccctctt
     3061 caagaacgtc tcgtccaaaa gtttctatgt ggaaacgaat agcagacttt aggcctgcta
     3121 aagcctcttc ataggtgtcg ccttcgccaa caacaatccc tttcaaacca agcggatagg
     3181 ctatataacc atcgggatgt ttttcaacta taactttgaa ttgtttcgcc ctgacactct
     3241 ccatctaaac acgattgtac actatttgat aaagtttcaa aatgataatt ccctgtagct
     3301 ttctacaggg tttccacaat gctatattgt cattgcgaac acgttcactt cgttcagtgc
     3361 aggctccgtg aagcaatctc tcattttaga ttgcttcgtc cccttcgact tcgctcaggg
     3421 tcctcgcaat gacagttgtg gcgctttaat attaagacca taccctgcag cttgttgcag
     3481 ggttattgac ttggtttgca cttgttcatt gcttgtttaa agaaaattta agaaataata
     3541 aattcgcaca ctggaaaaag catactggca aaaaccttgt ttgacataat atagctgcag
     3601 ttataatatc ttgtgctaaa gagaaaatta attcatctaa acaaaggaga agcaattatg
     3661 agaaacttaa cctatcttat tatgagtgtt ttcttagcca cctttgcgtt gatctttgcc
     3721 acggagacgg cgatcgcaca agacgctgtg aaggcagatc caaatcacta caaagtggag
     3781 ttcgagaatg accaggtgcg ggtgctgcgg attagctacg ggccacacga gaaatctgtg
     3841 atgcatgaac atccagatag cgtagcgatt ttcctgactg accaatacgt gaaatttacc
     3901 tcaccggacg gaaagagtga ggaaatgcgt actaaggctg gagatacgag atggcttcct
     3961 ggaggaaaac acctgcctga aaatttgagc gaccaacccc ttgagttaat tttggtggag
     4021 ttaaagacta tgccagcaat gactaagtag ggatagtcca cccaagcaga gaaagagggt
     4081 accactaaag cttgtattaa ttatcaaatg agtattattt catttgctca tttcttcata
     4141 agggtaaatg ttttaacatg tccccctttt ttactaattg tcggacagcc tgcttgctgc
     4201 agggttcttc attcaattaa taactggaaa tggtattatt tgattataat ataattttga
     4261 tgaatttatt aatctgaaga atatagatgg aaaacctaag tcagcttaat attccattct
     4321 taaaagaacc tagagcggtt gacgctctgg agtctaaaaa actcaagtgg aacgtttcac
     4381 ttatagagtc tcgttcaatt cttagtaaga ccggaatatc atgttttgat tatgtaataa
     4441 attgctacag gggatgcagt ttcgggtgtc ttcattgcta tgcttctttt atgaaaaaat
     4501 ttactggtca taaggaaaag tggggggatt ttgtagatgt gaaggttaat tctgtagagt
     4561 tacttaaaaa agaaataaaa aagataaata aagggagggt atttttaagt tctgtaacgg
     4621 atccatactt gccaatcgaa gcaaagttta agctcactag gggcgtactt caatgcttca
     4681 ttgatacgga ctttgaaata aacatactca ccaggtctcc tctagtcaca agggacatag
     4741 atatttttaa aaagcttaaa agcgttgagg ttggactgag catcccaacc gatagagagg
     4801 acgtaagaaa gattttcgag cctaatgcaa cgcctatttc ttccagaatt aaggcgctag
     4861 gaaagctaaa agaagagggg ataaacacgt atgcctttat agctcctctt cttcccttaa
     4921 atccagaaaa tctagcaaaa ctcctcaatc ctatagccga ttacgtgatg ttagatggtc
     4981 ttaattattc atggaaggta tcagatatta ttcgaaaaca tagattcgaa tatatacttg
     5041 atcccaagtg gattcgtgaa gtagaggaga aatttcaaag gctgcttggg gggaaatttt
     5101 cttgattttc gctttacttt tgtttaaaat gtaaagcgag gagaattgct atgtatgagg
     5161 ctaaactatc gagcaaaaat caaatagtaa tcccgcgtga tgccaggaat gctcttcgta
     5221 ttaaggcagg cgatagactg ctggttgtgg ttcgtggcga tactgttatt ttgcttcgta
     5281 agccgaagaa ttactcgaaa gctattagtg aaactgctaa ggggatatat tcgtccgatt
     5341 atctggaaaa ggaaagggaa agctggcggt gagaagaaag aggctcgagg attttttcga
     5401 gcaacaccgc aagattggac ttgataccag tgttttcatc ttcgaaatag aagggaatcc
     5461 caggtatata aaactcgtaa acccgatttt tgtcaggctc gatggaccga agggtcgtgc
     5521 tgttacttcg acaatcacaa tgctggagct tctggttcag ccctatagac tttcggatat
     5581 tgatagcgta aataagttct acgctctgct ttctacctat ccacacctag agtggatcga
     5641 accaacactc gagattgctg acctagcggc acgattgagg gctgaatgca atttgaaaac
     5701 acccgatgct ttgcaagcgg cgacggcatt agcccatcag gcaacaggat ttatctcgaa
     5761 cgacactgca tttaaaaggg tgggcggttt ggaagtatta attttagatg agcttctttt
     5821 tgtttagtag ttcctggcgt ccagtttgga ctaaagccgc tttactcgat cttaatctct
     5881 tatttgaccg aagcggtatt gattctttat gagttacgga gaataatatt aacttgaatt
     5941 cgacttgagt gtgattatag cggaattctt ccttcgataa aactcaggac aggttaggat
     6001 tccattcccg attaagacat tcgggaatga caataaaaac ggaggtctaa agaccccagc
     6061 tacattagtt cttcttttga cagggcaggt ggcggaacag gcagacgcga cggcctcaaa
     6121 agccgttggg cttcgcgccc gtaagggttc gactcccttc ctgcccattt tggtatattt
     6181 ctaatatcta ggcatgtctt ggtcatattc ttgcgcccaa acatctatac cgccttttag
     6241 gctttttacc tttttgaacc ccatgtttct taagaacgtt gttgcccgaa ggcttctcac
     6301 accatgatgg cagtggacta tgatttcatc cgcggtgtca agctcattta ctctcgaggg
     6361 caaatcccca agcgggatt
//
LOCUS       DATDRL010000134         4859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_294886, whole genome shotgun sequence.
ACCESSION   DATDRL010000134 DATDRL010000000
VERSION     DATDRL010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294886"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4218)
                     /locus_tag="VI935_04860"
     CDS             complement(<1..4218)
                     /locus_tag="VI935_04860"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HLE24964.1"
                     /translation="MNSRILFLSVIFSIFSSFNLAYSEKDAYVEIFSPQGTVKGVRQV
                     TARFSEQMLPFGEPRFVEPFAINCPEKGRARWVDGKTWSYDFDKGLPAGIRCDFILKE
                     GLKTLSGNEVKGQNRFSFSTGGPAIIESYPWEGSESVDEDQIFVLTLDAKTNENSILS
                     NVFFSIQGINERVGVRIIKGNERERILKSIGYRDDNTTRVLLQSKQRFPNNSVVRLVW
                     GKGVMSLTGVKTAEDQVLNFKTRGPFTATFSCERENPEADCIPILPMRLEFSSSVSWK
                     YGSKIILKGPNNKTYMPSKSDDNTEFVYSITFKPPFPEKASFTIEIPKDIKDDAGRNL
                     ANIDKFPLKVMTDAYPPLAKFAARFGIIELKGDGTLPLTLRNIEPELIAKKLGIEDKK
                     VGIVEKVKTWILENLKGRIFKVDVGDDKEIIEWLKIVAGAKRENSIFQDKKATRNFVI
                     PKPGGRKAFEVVGIPLKEPGFYVVEIESEILGASLLGKRKPMYVQTVALVTNLSAHFK
                     WGRETSLVWITTLDKAQPVNNASVLISDCKGNLIWNGKTDKNGIAKINTKLPSEQNLP
                     HCEFHINYREASQALEGIGSGLFIFAKTSDDMTFVHSSWDKGIEPWRFNLPGGSYSEG
                     EGIITHTIFDRTLLRAGETIHMKHILRKHTIAGFEMVPDKEKPDVMVIDHQGSEQKYR
                     FPLKWNPNGSTEAQWEIPKDVKLGFYNVVLLRKGNEDDYSKQLRSGSFRVQEFRVPLM
                     KGIIQFPSEALIKPKEVDVDISVSYLSGGGASNLPVKLRSEVQPRYLPTFEGFDGFQF
                     SNGRIKEGIQRSSDYESEGEEGYTVSRKNVKLKTLALTLDKAGAARAKLSGLPQIDTP
                     REILTELEYKDPNGEIQTVAARIPLWPSKVLVGIKPDSWALSKDSLKYQVAVVDFTGK
                     AVPNALVKVDLFQRKTYSHRKRLVGGFYSYEHITEIKRLGQICKGKTEKKGILFCNNS
                     ISVSGSVILQAETTENLGNTSTANYEVWIAGEDDWWFQAGNEDRIDLLPENKRYEPGQ
                     TARFQVRMPFREATALITIEREGIVDAFIKNISGKEPVIEIPVKGSYAPNVFVSALVV
                     RGRVSDVQPTATVDLGKPAFRLGITDINVGWKSYELKVGVSTNKSVYKIREKIEAKVD
                     VKTADGKLPPRGSEVAVAAVDEGLLELMPNTSWKLLDAMMGTRGYEFRTSTAQMQVVG
                     KRHYGLKALPQGGGGGKQITRELFDTLLFWKANVALNEKGEANVEIPLNDSLTSFRIV
                     AVATGDVGLFGTGGTSIRTTQDLIVLSGIPPLVREGDKFRAGFTVRNASDRKMTVEVK
                     GGIRDNKEKKELVMITEPLKPGEAKEIGWDINVPYNTDSLTYEVMVKEKDSRGTLQRA
                     PTSDSLKVTQKVIPAVPTRVFQATLTQI"
     gene            4550..>4859
                     /locus_tag="VI935_04865"
     CDS             4550..>4859
                     /locus_tag="VI935_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLE24965.1"
                     /translation="MPEELYKKSDVKKLRKVRELTPESFKAYVDFDKQALSEGALPQK
                     IKRLIAIAVAHTTQCPFCIDFHTKEAKKLGATEQEIIEAMWVSAALRAGAAVAHSVLA
                     V"
BASE COUNT         1288 a         1114 c          866 g         1591 t
ORIGIN      
        1 tatttgcgta agggtggctt gaaaaaccct tgtagggact gctgggatca ccttctgggt
       61 aaccttgagg ctgtcggagg taggggcacg ttgcaacgtg cccctactat ctttctcttt
      121 taccataacc tcgtaggtca agctatcagt attataaggt acattaatat cccaaccgat
      181 ctcttttgct tcacccggct ttagaggttc agtaatcata actagttctt ttttttcttt
      241 gttatctctg atgccccctt taacctctac agtcattttg cgatcagagg catttctaac
      301 tgtaaagcct gccctaaatt tgtctccttc tcttacaagt ggtggaatac ctgaaagaac
      361 aatcaaatcc tgagtcgttc ttatgctcgt tcctccggtg ccaaatagcc ctacatcacc
      421 agttgcaacc gctacaattc tgaaactagt tagagagtca ttaagtggta tctccacatt
      481 tgcttcaccc ttttcgttta gagctacatt cgctttccag aataaaagag tatcaaatag
      541 ctcgcgtgta atctgctttc cccctcctcc accttgaggg agtgccttta gtccataatg
      601 tctttttccc actacctgca tttgagcagt agatgttcga aactcgtatc cccttgttcc
      661 catcattgca tcaaggagtt tccaactggt attgggcata agctctagaa gaccttcgtc
      721 aacagcggct acggccactt cacttcccct tggcggaagt tttccatcgg ctgtttttac
      781 atctactttc gcttctattt tttctcgtat tttatagacg cttttatttg tagaaacccc
      841 tacctttagc tcatatgatt tccatccgac attaatatcc gtaataccaa gcctaaaggc
      901 tggttttcca agatcaactg ttgctgtggg ttggacatca gaaacacggc cacgtacaac
      961 tagagcagat acaaaaacat tgggcgcata actcccttta acagggattt ctataacagg
     1021 ctcttttcct gatatgtttt taataaatgc atctacgatt ccttcccttt caattgtaat
     1081 aagtgcagtt gcttctctga atggcatcct cacctgaaat ctagcagtct gccctggttc
     1141 atatcttttg ttttctggta ataagtctat gcgatcctcg tttcccgcct gaaaccacca
     1201 gtcatcctct cctgcaatcc agacctcata gtttgctgtt gatgtattac ctagattttc
     1261 cgtagtctca gcctgcaaga taacacttcc agatacggat atagaattat tgcagaacaa
     1321 tatccccttt ttttctgttt tgcctttgca gatctgacct aagcgtttta tctcagttat
     1381 atgctcatat gaataaaatc cgcctacaag tcgttttcta tgggagtagg tctttcgctg
     1441 aaagagatct accttaacca gcgcattggg tactgccttt cctgtaaaat caaccaccgc
     1501 aacctgatac tttaaggaat ccttagatag agcccatgaa tcaggcttta ttcctactag
     1561 cactttggag ggccataatg gtattcttgc cgccacagtc tgtatctccc catttgggtc
     1621 tttatattca agttcagtta aaatctctcg tggagtgtca atctgcggaa gccctgagag
     1681 ttttgctcta gcagcccctg ctttatctag tgtcaatgca agggtcttaa gcttcacatt
     1741 ttttctacta actgtataac cctcttcacc ctcagattca tagtctgagc ttctctgtat
     1801 tccttctttt atacgtccat tggagaattg aaatccatca aatccttcaa atgtagggag
     1861 gtatctcggt tgaacctcgc ttctaagttt tacaggaaga tttgatgcgc ctccccctga
     1921 aagataggat acagaaatat ctacatctac ctcttttggt ttaatcagag cttctgaagg
     1981 aaattgtata atccccttca ttaaagggac gcgaaactcc tgaaccctga atgatccaga
     2041 tcttagctgc ttactatagt catcttcatt ccctttcctc agtagaacaa cattatagaa
     2101 tccgagcttt acatcttttg ggatctccca ttgtgcctcg gtcgagccat ttggattcca
     2161 tttaaggggg aaacggtatt tttgctcact cccttgatga tctataacca tcacatcagg
     2221 tttttctttg tcagggacca tttcaaatcc agcgatggta tgctttctca ggatatgttt
     2281 catatgtatg gtctctccag cacgaaggag tgtacggtca aatatggtat gcgtgataat
     2341 tccttcaccc tcagaataag acccacctgg aagattaaat ctccatggct ctatcccctt
     2401 gtcccagctt gaatggacaa aagtcatgtc atccgatgtc tttgcaaaga taaataatcc
     2461 actacctata ccctctagtg cctgcgaagc ttctctgtaa tttatatgaa actcacagtg
     2521 agggagattt tgttctgaag gtaatttagt gtttatttta gcaatgccgt ttttatctgt
     2581 ctttccattc catattaaat ttcccttgca gtcacttatc aaaacagaag cattatttac
     2641 aggctgagct ttatccaaag ttgtaatcca tactagtgat gtttccctcc cccacttaaa
     2701 atgcgctgaa agattcgtta caagggcaac cgtttgtacg tacataggct tccttttccc
     2761 aagcagagat gcacctagga tctcgctttc aatctctaca acatagaagc cgggctcctt
     2821 taatggaatc ccaacgacct caaacgcctt tctaccgccg ggtttaggga ttacaaaatt
     2881 ccttgttgcc tttttgtctt gaaagataga gttttcacgt ttcgcgcctg ctactatttt
     2941 taaccactct ataatctctt tgtcatcacc cacatcaacc ttaaaaatcc ttccctttaa
     3001 attctcgaga atccatgtct tcaccttttc aacaattcca acttttttat cttcaattcc
     3061 tagctttttt gctataagtt ctggctctat atttctaagg gttagaggaa gcgttccatc
     3121 tcctttaagc tctataatcc cgaagcgtgc tgcaaacttt gcaagtggtg ggtaggcatc
     3181 tgtcataacc ttgagcggga atttatctat atttgctagg ttccttccag catcgtcttt
     3241 tatatctttt ggaatctcaa tagtaaatga agccttttcg ggaaatggag gtttgaatgt
     3301 tattgaataa acgaattccg tattatcgtc tgatttcgac ggcatatagg ttttattgtt
     3361 aggccctttt agtatgattt tacttccata tttccaagaa acagaagatg aaaattctaa
     3421 acgcatgggc aatataggaa tgcaatctgc ctctggattt tccctttcac agctaaatgt
     3481 agctgtgaaa ggtccacgag tcttgaaatt gagtacctgg tcttcagcag tttttactcc
     3541 agtaagagac attaccccct ttccccaaac taaacgaact accgaattgt ttgggaatct
     3601 ttgcttggat tgaagaagaa ccctcgtggt attatcatct ctatatccaa ttgatttcag
     3661 gattctttct ctctcattac ctttgataat ccttacaccg acacgttcat tgattccttg
     3721 tattgagaag aagacgttag agaggattga gttctcattc gtttttgcat cgagagttag
     3781 gacgaatatc tgatcttcgt ctacactttc agaaccttcc cagggatagg attctattat
     3841 cgcaggtcct ccggttgaga aggaaaatct attttgtccc tttacctcat tgcctgatag
     3901 tgtctttaaa ccttctttta gaatgaaatc acatcgtatt cctgctggaa gacctttatc
     3961 aaaatcataa gaccaggtct ttccatcaac ccatcttgcc cgtccttttt caggacaatt
     4021 tatggcaaat ggttcaacaa agcgaggttc gccaaatggc aacatctgct ccgaaaacct
     4081 agccgtaact tgtctaacac ccttgactgt gccctgaggt gagaatatct ctacatacgc
     4141 atctttttcg gaataggcaa gattgaaaga agaaaatata gaaaaaatta cagacaaaaa
     4201 taggattcga gagttcaatt ttcttctcct tttcattttc cactatataa agttaggcaa
     4261 aaagtttatc aaattccgag tagaaacacc acatatttgt tgttccattc aattgaaatg
     4321 ctttgttgta ggcatttata atgtaatccc accagcaaga ctggtggact tatttttgtt
     4381 tcgtgttttt gatttttggg taggtcacga gtcttgctcg tgaccgcttt tcactattta
     4441 tgcttttatt tcacaatgaa aatggtatta attccctaat acgttaaaaa cgggtagcat
     4501 ctttaacttc aaaaagaatc taatattcat aacttcaaag gagataaaaa tgccagaaga
     4561 actttataag aaatccgatg ttaaaaagct taggaaagta agagaactta ctcccgaatc
     4621 tttcaaggct tatgtagact ttgacaagca ggcgttgtct gagggtgcac ttcctcaaaa
     4681 aataaaacgc ctcatagcaa tagctgtcgc tcatacaact caatgcccat tctgtatcga
     4741 ttttcataca aaggaagcaa agaagcttgg ggcaactgaa caggaaatca ttgaagccat
     4801 gtgggtttct gccgcactcc gtgccggagc cgcagtagcg cacagcgttc ttgccgtta
//
LOCUS       DATDRL010000135         8180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3459453, whole genome shotgun sequence.
ACCESSION   DATDRL010000135 DATDRL010000000
VERSION     DATDRL010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8180
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3459453"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VI935_04870"
     CDS             <1..301
                     /locus_tag="VI935_04870"
                     /inference="COORDINATES: protein motif:HMM:NF037014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24966.1"
                     /translation="KSVFRILGEDGRFRKDVITEEGGKPNSGNYYPLLIKVVENGNII
                     YDPPSLPKIRDRFMEKLRRLPDDLKKIDEVHDYKVEISHSLKDIIKRIRSKTMGN"
     gene            complement(298..915)
                     /locus_tag="VI935_04875"
     CDS             complement(298..915)
                     /locus_tag="VI935_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HLE24967.1"
                     /translation="MEYVFSQGEKLVKIARKVVEDELFDKKHDIPKWFTKEFKEDRGV
                     FTTIRTTPESELRGCTGFPLPTQSLWKALVKSSLQTAFNDNRFPPLTIHELEMVTFEV
                     TILTTPERLMVESPEEYIKTIVIGKDGILIRLGVYSGILLPQVPVKERWDVETFLNAV
                     CYKALLPIDSWRNLKTEIYRFQGKIFGEKHPRGEIVEHSELVNIY"
     gene            1064..2665
                     /locus_tag="VI935_04880"
     CDS             1064..2665
                     /locus_tag="VI935_04880"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HLE24968.1"
                     /translation="MILDMFRRSQAWFTKGILIVLAITFAFGFGFSFSNFGFGGKVPE
                     GTAAAVNGEKISLLDFYRARERIRSQYRESGVPEEALNQNFIDMTALNQLIDLRLLSQ
                     KAKELGFRVTDEELSEAIRSNPVFQVDGQFIDANEYRAFVEQRLNQSVSDFEKSYREE
                     LLAQKLVKLMFETFNVTDEELFNTYKTRNEKVNLNFVVISPEDFKDYYTPNMEEIKKY
                     YENNKSEFKTSELRSVRYLTISPDDFIDRVDVSQEEIKSYYEAHPDKFRSNENESRPL
                     SEVKGEVEVDIKKEGGKALFREFLENLKESVDKKSIDEITKENGLGEIKVIGPFSVTE
                     KLDDIPSKVVEKAFTIKQGEKAFAQVGEKIYIIQVYDVIPTREKTLNEAEGEISERLR
                     SMKGKEVAKAKANDLLNRAKINGGNLEEVAKTLGLKLQETGYFSRLEGVPEINSDEIN
                     KDAFFLEKKNPLASKPYEADEKIYIVSLKEEQDVDPKRFEEEKDQIREEELSQRRREL
                     YIDWIQRLRQESKININEKLLQSQG"
     gene            2962..6492
                     /gene="smc"
                     /locus_tag="VI935_04885"
     CDS             2962..6492
                     /gene="smc"
                     /locus_tag="VI935_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HLE24969.1"
                     /translation="MKIRSLELIGFKSFYEKTTIQFQDGINAIVGPNGCGKSNILDAI
                     RWVLGEQNPRRLRAEGMEEVVSNGGETSKPLGMSEVSLELTDVSHSGYEQVVIKRRLF
                     RSGESEYYINGTPCRLKDITEMFLDTGAGARAYSTVDQGKVEHLVTAKPEEKRTLIEE
                     AAGIVRYRVRRRETERKIESTKENLRRLKDVMDEINRQMGSLSRQARDAEGFKRLSEE
                     ARSLEFRIIYSKLYKMEAERNRLLQEKSELEEQVLSLSQQIKNRESIHENIEARTNLV
                     ERKLEELGKEIYDLKSELQAKEALEGLVRSEVSGIDELIKRLEKGIVLLLEETERANI
                     QINLKQKILEEVKADLSNKEHELKLREEVLSEIRAGFTKNQTDLEGTRTSLFEVLDKY
                     SSLRGSALGYERELSELRSRRQRTEKEIGEVEFEKKKTISRISELQGYVNANEERRIK
                     VEGRKEAIGLSLATLSATQESKRNEIEGLSERFKEAQSRLNVLKQIETSYEWLPEGIR
                     RFLLEKKGDGILGVIADFISVPKGYERALEAAFGEKLNWGIVRESEEALSAVEYLREL
                     SLGRGTFVPINNARKIEVLDKNGNGIVPLSDIVQVEGIDKDIIDNMLTNVFVVSSLRE
                     ALNLKDAIQNGASFVTLGGDMLDSTGAISGGLTIEGVFERKREIEELTIDTQNLESGL
                     SRISKEIESNQEEVEKLKLTLRDLENDLIEIEIKGAEIKADNTNLQDNIAKTNMRYEV
                     VEFDLNEIDSEILDREGKLQEAQEAIKQVEDQKIFLEEKYAKLKEGAEKLEEEEKHIE
                     RKITNLKIELAALIEKEKGIDEDLVELGRRQNDIRERIELEANEVEKKKQEKLNFIKT
                     EEDAKRDVKGLLSLLGQKDEELLVKRNQKDELLLQIRTAIEDKQRLNEEIKGHEKKIN
                     SLDLRLNSLQIEIEHLRETIRENSSYPGLDDDNEAFRSIDPEGLKGLVTEGEEERLLK
                     LKEKIERFGPVNLLAPEEYKNLEERFKFLGAQNEDLVNAISSLKKAMNKIDRESEKRF
                     NATFEVMNKKFQEVFSRIFRGGEAKLVLTNSEDLLETGVDVMVRPKGKRFQSVSLLSG
                     GEKALSAIALVLSACFIRPAPFLLFDEIDAPLDDVNTSQVTDLLEEATKDSQIIIITH
                     NKKTMQVARTLVGITSDGRSTSKVVSVELK"
     gene            6649..7587
                     /locus_tag="VI935_04890"
     CDS             6649..7587
                     /locus_tag="VI935_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24970.1"
                     /translation="MANLFWKTKAGTKSLLETPFKTEEEFESTVFNTSELLEEIFLLR
                     RQIRGGSKTGIPDIVGIDGDGNICIIEMKNKPVDASIIPQVLEYALWAETNPDSIKSL
                     WLESENKPDDIPINWEDFDVRILIIAPYIHRSTLEFVQKINYLVDLIEIKKWAEENSQ
                     ILMVNKLEEEKSRTKPVRGLQKYDEAFYKSKYNKTSVGQFFKYVKEVEKTIKKKGWSL
                     DTKYNKNYCAFKAGFFNAFRISWMGAKSFASTFRIPEKETKKIKIPITKYDPERHRAY
                     YYIEPDITKVNDFIPLFEYCYKQIAGDSGKRSRLAN"
     gene            7757..>8180
                     /locus_tag="VI935_04895"
     CDS             7757..>8180
                     /locus_tag="VI935_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24971.1"
                     /translation="MFSYIMKKIIGTQNEREINKLRSLVLHANEIEPELIKLPTSELR
                     GKTDYFKESIKNQLNDHHKLDEGEYFKKMEEILWDILPEAFAIVREASRRTLGMRHFD
                     VQIIGGIILHQGKITEMKTGEGKTLVAALPLYLNGITGF"
BASE COUNT         2864 a         1273 c         1839 g         2204 t
ORIGIN      
        1 gaaatctgtg tttaggatcc taggtgaaga tgggagattt cgaaaagacg tgataactga
       61 agagggagga aaacctaact caggaaacta ctatccactt ttgataaagg tggtggagaa
      121 cggaaatata atctacgatc ctccttcgct tcccaagata agggatagat tcatggagaa
      181 acttagaagg ctccctgacg atttaaaaaa gatagatgag gtacacgatt ataaagttga
      241 aattagccac tcgcttaagg atattattaa acgaataagg tcaaagacga tgggtaatta
      301 gtaaatatta actagctcac tatgttctac aatctcaccc ctagggtgtt tttctccaaa
      361 tatttttcct tgaaatctgt aaatttcggt ttttaaattc ctccagctat ctataggaag
      421 tagagcttta tagcatacag cattgaggaa ggtctctacg tcccaccttt ctttcacagg
      481 aacctgaggg agaagaatgc cggaataaac ccccaatctt atcaggattc catctttccc
      541 aatcactata gtttttatgt attcctctgg tgattctacc ataagccttt cgggtgttgt
      601 gagaatcgtg acctcaaagg tgaccatttc taactcatgg atggtaaggg gtgggaatct
      661 attatcatta aaagcagtct gtagtgacga tttaacaagt gccttccaga gggactgggt
      721 tggtagagga aagcctgtac atcctctaag ttcagattcg ggcgttgttc taatagttgt
      781 aaaaacgccg cgatcttctt taaactcctt agtgaaccac tttggtatat catgtttttt
      841 atcaaaaagc tcatcttcca ccaccttcct agctatcttt actagtttct caccttgtga
      901 aaaaacatat tccattactt aattattata gattgaaaaa ttaaaaaaat taatgccctg
      961 gatatcttat ttatccgaat tttgtccttc tatctaattc ttgaaacttg tggacaagtg
     1021 gtatagattt aaataaaaaa attagtaaaa ggcgatatta ataatgattc ttgatatgtt
     1081 tagaagaagt caggcatggt ttacaaaagg gatcttaatt gttcttgcta ttacctttgc
     1141 tttcggattc gggtttagct tcagcaactt tggctttgga ggaaaggttc ccgaagggac
     1201 cgctgctgcg gtaaatggtg agaagatatc acttttggat ttttataggg ctagagagcg
     1261 tattcgttct cagtatcgtg agagtggagt tcccgaagag gctttaaatc agaattttat
     1321 tgatatgaca gcactaaatc aactcattga cttgcgacta ctttctcaaa aggctaaaga
     1381 gcttggcttt agggtaactg atgaggaact aagtgaagca ataaggtcta acccagtttt
     1441 tcaggtagat gggcagttta ttgacgctaa tgaatacagg gctttcgtag agcagagatt
     1501 aaatcaatct gtaagcgatt tcgagaaaag ttatagagaa gaactacttg ctcaaaaatt
     1561 agtaaagcta atgttcgaaa catttaacgt tacagacgaa gagctattta atacctataa
     1621 aacccgcaat gaaaaggtaa atcttaattt tgttgtcatc tcacctgaag attttaaaga
     1681 ttattacaca ccaaatatgg aagagataaa aaaatactat gaaaataata aaagcgagtt
     1741 taaaacatct gaacttagga gtgttcgcta ccttactata tctccagatg attttataga
     1801 tagagtggat gtgtctcaag aagaaataaa gtcttattac gaagcacatc ctgataaatt
     1861 tagatcaaat gaaaatgaaa gtcggcccct ttcagaggta aaaggcgagg tggaagtgga
     1921 tattaagaag gagggtggaa aggcgctatt cagggaattc cttgaaaatc ttaaagagtc
     1981 ggttgataaa aaatccatcg atgagataac aaaggaaaat ggtttaggag aaataaaagt
     2041 tataggtcca ttttctgtta ctgaaaagct tgacgatatt ccatctaagg tagttgaaaa
     2101 ggcgtttacg attaaacaag gagaaaaggc ctttgctcaa gtgggagaga aaatatacat
     2161 tattcaggta tacgatgtta tcccaactag ggaaaagacc ctgaatgagg cagagggtga
     2221 aattagcgag cgcctccggt ctatgaaggg aaaagaggtt gctaaggcaa aggctaatga
     2281 tttacttaat agggcaaaaa taaatggagg gaatttggaa gaggttgcaa aaacccttgg
     2341 tcttaagtta caggagactg gttatttttc cagacttgaa ggtgttcctg aaataaactc
     2401 ggatgagata aataaggatg cgtttttcct tgagaaaaaa aatcctttgg cttctaagcc
     2461 ttatgaagcc gatgaaaaaa tttatatagt atcgcttaaa gaagaacaag atgtggaccc
     2521 caaaaggttt gaagaagaaa aggatcagat aagagaagaa gaactttctc aaagaagaag
     2581 agagctttat atagactgga ttcaaagact caggcaggag tcgaaaatca atatcaatga
     2641 gaagcttttg caatctcaag ggtgataaat caagtagtat gattctaggg atcggaggaa
     2701 aaggtagcac ataaacattc tattacccaa ggctattggt caatttttgt tgtttagaac
     2761 aaaatttaat ctcgacaata cattataggt agagctttaa tattgtactt taaaatagaa
     2821 gaaaaaaatc agcttttgat ccaatttttc aaaaattaaa ttaacgcacc atatatggta
     2881 tttcttttct taaacaccac agcagtttgt gtatgtttat ttacaaccta ttatgattat
     2941 gatattattc aattttaaag aatgaaaatt aggtctttag aacttatagg ttttaaatcc
     3001 ttctatgaaa aaactactat tcagtttcag gatggaatta atgccatagt cggtcccaac
     3061 ggctgtggaa aatccaacat actggatgca atacgttggg ttcttggaga gcagaatccc
     3121 aggcgcctaa gagcggaggg gatggaagag gttgtttcta atggggggga gacctcaaaa
     3181 ccccttggta tgtcagaggt ttccttagag cttacagacg tttcgcatag tgggtatgag
     3241 caggttgtaa tcaagcgaag actctttcgt tctggtgaga gcgagtatta cataaacggc
     3301 acaccttgca ggttaaagga cataactgag atgtttttag atactggcgc aggcgcaagg
     3361 gcctattcta ccgtagatca aggaaaggtg gagcatctag ttacagcaaa acctgaagaa
     3421 aagaggacat tgattgaaga ggctgcaggg atagttagat atcgggtaag acgccgtgag
     3481 accgagagaa agattgaatc tactaaagag aatctgagac gcttaaagga tgttatggac
     3541 gaaataaacc ggcagatggg tagcctaagc cgtcaggcaa gggatgcaga ggggtttaaa
     3601 cggctttcgg aagaagccag gagccttgag ttcaggataa tctactcaaa gctttataaa
     3661 atggaagcag aaagaaatag gctccttcag gagaaatctg agctcgaaga acaagtcctt
     3721 tcactttctc aacaaataaa gaacagagaa tccatacatg aaaatattga agcaaggacc
     3781 aatcttgtcg aacgtaagtt agaagagttg gggaaagaga tctatgatct caagtcggag
     3841 ctccaagcaa aggaggcgct tgaaggacta gtaaggagtg aggtttcggg gattgatgaa
     3901 cttattaaga ggttggaaaa aggaattgta ttactactgg aagagaccga aagggcaaat
     3961 attcaaatta atctcaagca aaagattttg gaggaggtta aggcagatct atccaataaa
     4021 gaacatgagc ttaagctaag agaagaggtc ctttctgaaa tcagagctgg gtttacaaaa
     4081 aaccagactg accttgaggg tacaaggaca tcactctttg aagtgttgga taagtacagt
     4141 tctctaaggg gtagtgcttt aggatacgaa agggaactgt ctgaacttcg atcaagaagg
     4201 caaagaacag aaaaagagat tggagaagta gaatttgaga agaaaaaaac aatatcacga
     4261 atctcggagc ttcaaggtta tgtgaacgct aacgaggagc gtagaatcaa agttgaggga
     4321 aggaaagaag ctatcggatt atctctcgct actttaagcg ccactcaaga gtcgaaaaga
     4381 aatgaaatag aagggctaag cgagcgtttt aaagaggcgc agtccaggct taatgttcta
     4441 aaacagattg agacaagtta cgaatggctt cccgaaggaa taaggagatt tttacttgag
     4501 aaaaaggggg acggaatctt aggggtgatt gcagatttta tttcagttcc aaagggctat
     4561 gagcgggcac ttgaggcagc atttggtgag aaacttaatt gggggattgt tagagaaagc
     4621 gaggaagcgc tgagtgcagt tgaatacttg agagagctat cccttggaag aggcacgttt
     4681 gtccctatta ataacgcaag aaagattgag gttcttgata aaaacggaaa tggaattgtg
     4741 cctctcagtg acatagttca ggttgaggga atagataaag acataattga taacatgcta
     4801 accaatgtat tcgtagtatc ttctcttaga gaggctctaa acctcaaaga cgcgattcaa
     4861 aatggtgcct cttttgtcac acttggagga gatatgcttg actctactgg tgcgatatcg
     4921 ggtgggctta ctattgaagg cgtgtttgag agaaaaaggg aaattgaaga actaacaata
     4981 gatactcaaa accttgaatc tgggctttca aggatttcaa aggagataga gtcaaatcag
     5041 gaagaggttg aaaaactgaa acttacgctt agggacctag agaacgactt aatagagatc
     5101 gagataaagg gagccgagat taaggcagat aatactaatc tgcaagataa cattgcaaaa
     5161 accaatatga gatatgaggt agttgaattt gatttaaatg agatagattc tgaaattctt
     5221 gatagagaag gaaaactaca agaagcacag gaggctataa aacaagttga agatcagaag
     5281 atatttcttg aggaaaaata tgcaaaatta aaagaaggtg cggagaagct tgaagaggaa
     5341 gagaaacata tagagagaaa aatcacaaat cttaagattg aacttgccgc gcttatcgaa
     5401 aaagaaaaag gcatcgatga agacctagtt gagctaggaa gaaggcagaa cgatataaga
     5461 gaaaggattg agcttgaggc caatgaggtc gaaaagaaaa aacaggaaaa actgaatttc
     5521 attaaaacag aagaagatgc aaaacgagat gttaaaggtc ttttgtctct tcttggtcag
     5581 aaggatgaag aactattagt taagaggaat caaaaggatg agcttttatt gcaaattaga
     5641 actgctattg aagataaaca aaggttaaat gaggaaataa aagggcatga aaagaagatc
     5701 aactctcttg accttaggtt aaatagcctt caaattgaga ttgagcatct tagagaaaca
     5761 attcgtgaaa acagttcata tcctggtctc gatgacgata atgaggcttt taggtctatt
     5821 gatccagaag gtttaaaagg tctagttacc gaaggggaag aggaaaggct ccttaaactt
     5881 aaagaaaaga tagagagatt tggtccggtg aatctccttg cgcctgagga gtacaagaat
     5941 ctcgaggaaa ggtttaaatt cttaggcgct caaaatgagg atttagtaaa tgctatctca
     6001 tcacttaaga aggctatgaa taagatagac agggagtctg aaaaaagatt caacgctacg
     6061 tttgaagtta tgaataaaaa gtttcaggag gtcttcagca ggatatttag aggcggcgag
     6121 gcaaagcttg ttcttacaaa tagcgaagac cttcttgaaa ccggtgttga tgtcatggta
     6181 agaccgaaag ggaaaaggtt tcaatcagta agcctgcttt caggcggaga aaaagccctt
     6241 tcagcgattg cccttgttct ttctgcgtgc tttataagac ccgctccttt tctactattt
     6301 gacgagattg acgcaccttt agacgatgta aacactagtc aggttacgga tttgcttgaa
     6361 gaagcaacaa aggattcgca gattataatt attactcaca ataaaaaaac catgcaggtt
     6421 gcaagaacac tagttggaat aacttctgat ggccgaagca catccaaggt agtttccgtt
     6481 gagctcaaat gatgttaaat ccctctttgt taaagaggga tttagggaga tttgtaaaca
     6541 caaatagtat ctgtccttat tttgtactta caacaattgg tctagcttta tactgttcca
     6601 aatgatatat aatgtaaaaa tactcatcaa agtaaaagga ctttaggaat ggccaaccta
     6661 ttttggaaaa caaaagcggg aactaaaagt ctgcttgaaa caccttttaa gaccgaagag
     6721 gaatttgaga gtactgtttt taatacatcc gagttactgg aagagatttt ccttttaaga
     6781 aggcaaatca ggggaggaag caaaacaggg atacctgata ttgtgggtat tgatggagat
     6841 ggcaatatct gcattattga aatgaaaaac aaacctgtag atgcttctat aatccctcaa
     6901 gtacttgagt acgcattatg ggcagagacc aatcctgact caatcaagtc gctctggcta
     6961 gagtctgaaa acaagccaga tgatattcca ataaactggg aggattttga tgtaaggatt
     7021 cttattattg ctccatatat ccatcgatca acactagaat ttgtccaaaa gataaattac
     7081 ttagtcgatt tgattgaaat taagaaatgg gctgaagaaa acagtcaaat attaatggta
     7141 aacaaattag aagaagaaaa gagtaggaca aagcccgtaa ggggtttaca aaaatacgac
     7201 gaggctttct ataaaagcaa gtacaataag actagtgttg ggcagttttt taagtatgtg
     7261 aaagaagtag agaaaacaat aaagaaaaaa ggttggtctc tagatactaa atacaataaa
     7321 aactattgtg cctttaaggc tggttttttc aatgccttta ggataagttg gatgggagct
     7381 aagagttttg catccacttt tcgaatacca gaaaaggaga caaagaaaat taaaatccca
     7441 ataacaaaat acgaccctga gaggcatcgt gcttattatt atattgagcc agatataaca
     7501 aaggtaaacg acttcattcc actttttgaa tactgctaca aacaaatagc aggagattcg
     7561 gggaagcgta gccggctagc aaattgatac acttcaagca tgaaataaat aactagggac
     7621 agacactatt agatttactt tctgctccat ccattcactt cagatacatt aaagaacgca
     7681 tacagtttat ccacttgtta taagggcgtt ttccacttat aatttttaac ggcttttgta
     7741 tttgtggagg ttttatatgt tttcttacat tatgaaaaag attattggaa cccagaacga
     7801 gagagagatt aacaaactac gttctctcgt tttacacgca aacgagattg agcctgaatt
     7861 aataaagctt ccaacctcag agctcagggg caaaaccgat tattttaaag aatcaataaa
     7921 aaaccaactt aacgaccatc ataaactaga cgaaggtgag tatttcaaga agatggagga
     7981 gatcctatgg gatatcctac ccgaagcctt tgccatagtc cgagaggcat ctcgaagaac
     8041 cctgggaatg cgccattttg atgttcagat tataggcggc attatccttc atcaagggaa
     8101 aatcacggag atgaaaacag gtgaaggaaa gactcttgtt gctgctttac ctctttattt
     8161 aaatggtatc actggctttg
//
LOCUS       DATDRL010000136         8533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1386398, whole genome shotgun sequence.
ACCESSION   DATDRL010000136 DATDRL010000000
VERSION     DATDRL010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386398"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1552)
                     /locus_tag="VI935_04900"
     CDS             complement(152..1552)
                     /locus_tag="VI935_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24972.1"
                     /translation="MGPLFDSDQDKTQKTQQVDALGPFITSKKSDTRFEYGFHPLFYL
                     VEDTEKDSTEFDFLYPLATYDRRVNDRRFQFIFYLLFYETVQTRSGFIENEFHLFPFI
                     FAKNAEDKNNSYFALFPVYGNLKNKFSRDEINFFLFPLFLRTTKNEATNYSFLWPFFG
                     YYTGGGQKGFRFWPLFGYRKKEGSFEEKFALWPIFASKRRVFYGGEIRSLSVLPFYSV
                     VESYQRSQTTYLWPFFNHLVDKRRGIERWDVPWPLLNFTRGKEEEETRIFPFYGSEKS
                     GRDQEGFFLWPLYRYDRGTFEDHKRTRNSVLFILYSDIKEEPIIEGGRSGRRIDFWPL
                     FTYKRDREGNANFHFLSIFEPFLSNNEGIERNYSSIWRLYQWKRYNDGRKTSSFLWNM
                     FKSESGKEGFKMNFQPIIPVFSYKYWEGESKVYFLGGLFGYNSDPYKKTIKLFYIPIN
                     ISSQENTTEEEVRKDE"
     gene            complement(1735..2907)
                     /gene="alr"
                     /locus_tag="VI935_04905"
     CDS             complement(1735..2907)
                     /gene="alr"
                     /locus_tag="VI935_04905"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HLE24973.1"
                     /translation="MRPTWAEIEINSLKSNFLKVKRIVGKDVGVISVVKADAYGHGSV
                     NVSKALIESGSNLLGVATVEEALELRGSGIDSPILLLSGIQLDEVEAMVKNNLTPSLF
                     SLASAHAINTYAEKIGKKVRYHLKVDTGMNRLGVGLSEICDFLENLLNCRNLELEGVF
                     THFANADVDSREPTLKQISVFNVMFSLINQAGLNPKYIHSANSAAIQRFPESHMNLVR
                     PGIMLYGAGRMGPCGEPFDRSTLSTSSGLTAQESPAADRTNFELKPVMKLKTRITQLK
                     RITAGTSVSYGGTFIASRPSIIATLPIGYADGYVRRLSNRARVSIRGFTAPVVGTVCM
                     DMTMVDMTEVPGAIVGDEVVLFGDEKVSAEDVARWADTIPYEIFSITGKRVPRVYI"
     gene            complement(2983..4503)
                     /locus_tag="VI935_04910"
     CDS             complement(2983..4503)
                     /locus_tag="VI935_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLE24974.1"
                     /translation="MDNRKSDQNIETDSFDRELFGELEATSEELKNLIERGSHLLPDF
                     RSLILDLFASFYKYNVITLPEDEVNRSVLMGRKLIEKALSSEDHKELREETILNDFNS
                     AIATMTLGGEIVKWVKSEYGLSEKSLIKEWELKKAEENLDELKDEVETWEEIEKKKSS
                     NKSLKEGKKKTQFELRSQEGELKDLQKEQKERLERMDLKLQNLVRSSLKQASERTEEV
                     EKELMEWGASMGMPEEKPIGEKLDLAEKLFKNEKLRRLALLVGGLKEEMFSSRRKIWS
                     KRGSEVYDISLGNDIGRIIPAELSFLRHKALRKDFLKRLIEGRLLQYYLKEEKGRGPL
                     IVCVDGSSSMEGNKEMWAKAVCLSLLEIARRQRRKFSVIVFSSKGTQLKVFGSGVKEN
                     WRMKEEEIIELAGYFPGGGTDFEDPLDKALEFLKESRFTRGDIVFITDGECDVRDEWL
                     KIFLDKKDKLKFKVFSVLIDLTGNETVESLKKFSDKVTAISNLTSKDARGIFLSLD"
     gene            4662..5042
                     /locus_tag="VI935_04915"
     CDS             4662..5042
                     /locus_tag="VI935_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24975.1"
                     /translation="MVEIHLPSAISTFTLIKRKDKTISDKQERQMAHINKFENALDEL
                     FKKRTSWLRSAIGRKKPGPPPKITRRFVKNGISNLQEIASNALTNKLAKSEFNKHALG
                     KRAWHSKGFGIDEKKSLFEEWFKK"
     gene            5512..6093
                     /locus_tag="VI935_04920"
     CDS             5512..6093
                     /locus_tag="VI935_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HLE24976.1"
                     /translation="MSVKKILMLVGDYVEDYEVMVPFQALLMVGHTVHAVCPGKKAGE
                     KVRTAVHDFEGDQTYSEKPGHNFALNATFDEVKAENYDALVIPGGRAPEYIRLNDKVL
                     DIVRHFSKANKPIAAICHAAQVLVAADVVKGRSCSAYYAVGPDIKRAGGKFMNIPVDK
                     AHVDGNLVTAPAWPAHPEWLAKFLKILGTTIKP"
     gene            6295..6809
                     /locus_tag="VI935_04925"
                     /pseudo
     CDS             6295..6809
                     /locus_tag="VI935_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            6936..7775
                     /locus_tag="VI935_04930"
     CDS             6936..7775
                     /locus_tag="VI935_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HLE24977.1"
                     /translation="MMKKEIKKVVRERYAKIVKQESSCCGTIESSCGSSNSDHEISKV
                     IGYSEEELKAVPEDSNLGLGCGNPVALASLREGETVLDLGSGAGFDCFLAANKVGKNG
                     RVIGVDMTPEMIEKARENARKGNYENVEFRLGDIENLPVADNSVDIVISNCVIVLSPN
                     KRRVFSEAFRVLKPGGRLMISDIVLLRQLPDFIKNSIEAYIGCVSGAMIKGEYIREIK
                     AAGFHDVKIINETSFAIDHVTSSQPASSIIDKFKIPPEKVTEIASSILSVRVYATKPL
                     QTQ"
     gene            8222..>8533
                     /locus_tag="VI935_04935"
     CDS             8222..>8533
                     /locus_tag="VI935_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLE24978.1"
                     /translation="MSKAKSKNDKEKKNTIANPRADEKNTTLKFEDILYEKKDRIARV
                     TINRPDMRNALRSKTREELAIAIEDAWVDDNIGVIVITGAGDKAFCAGGDLSWITDPK
                     KK"
BASE COUNT         2656 a         1786 c         1656 g         2435 t
ORIGIN      
        1 gataagggca gctactggga gtgtctgatt tccgatttct agcatctgtc taaatatctt
       61 ctctcgatta ctaagagcag ccttgcacca aaagatgctc tccataagaa gctgaagaat
      121 ctctccagtg gtatgaaaaa acgcagttat gctattcatc ttttcgaacc tcctcctcag
      181 ttgtgttttc ttgagaagaa atgttaattg gtatataaaa taattttatt gttttcttat
      241 aagggtcaga attatagcca aagagtcctc caagaaaata aaccttcgat tcaccctccc
      301 agtacttgta agaaaagaca ggaattattg gttgaaaatt catcttgaaa ccttcctttc
      361 cactttcact tttaaacata ttccatagaa aagatgaagt ttttcttcca tcattatatc
      421 ttttccactg gtataacctc cagattgaag aatagtttct ttctataccc tcgttgtttg
      481 agagaaaggg ttcgaagatg gaaagaaagt gaaaattagc gtttccttct ctatctcttt
      541 tataggtaaa tagtggccag aaatctatcc ttcttccgct tcttccccct tcaataatcg
      601 gctcctcttt tatatctgaa tagagaataa aaagaacgct gtttctagtt cttttatgat
      661 cctcaaatgt tcctctatca tatctataaa gcggccatag aaagaaaccc tcttggtccc
      721 tcccgctttt ctcgcttccg taaaagggga agattcttgt ttcttcctct tcctttcctc
      781 ttgtaaagtt aagaagaggc catggcacat cccacctttc aattcccctt ctcttatcca
      841 caaggtgatt aaaaaagggc cagagataag tggtttggct tctctgatat gattctacaa
      901 cagaataaaa aggcaaaact gaaagtgacc ttatctctcc gccataaaaa acccttctct
      961 tagaggcgaa aataggccag agggcaaact tttcctcaaa cgatccttct ttttttctat
     1021 acccaaaaag gggccagaac ctaaaaccct tttgaccacc gccagtgtaa tagccaaaga
     1081 acggccagag gaaactatag ttggtcgcct cgttctttgt agtcctaaga aaaagcggga
     1141 agagaaaaaa atttatttca tcccttgaaa atttattttt taagttccca taaaccggaa
     1201 acaaagcaaa gtagctatta tttttatctt ctgcattctt tgcgaaaata aatggaaaaa
     1261 ggtgaaattc attctcgata aatccactcc ttgtttgaac ggtttcgtaa aaaagaaggt
     1321 agaaaataaa ttggaatctt ctgtcattta ccctcctgtc ataagtagca agtgggtaaa
     1381 gaaaatcgaa ttctgtagaa tccttttccg tatcctctac aagataaaac agcggatgaa
     1441 agccatactc aaatcttgta tcgcttttct ttgaagttat aaatggaccc aaggcatcta
     1501 cctgttgagt cttctgagtt ttatcttgat cactatcaaa gagcggaccc aaactgaaac
     1561 cctcttctcc tgagcaagga tattcaccga taaatattag aataaataaa gaggcataaa
     1621 aaattttgga taaccattta agtatttgaa acatgagtat ttaaccctaa cttcaaaaga
     1681 atggaaaatc taggaaaatt ttccgtaggg acgagcggcc gcccctacta atcatcaaat
     1741 ataaacccga ggaacccttt tcccagttat tgaaaagatt tcgtagggaa tggtatctgc
     1801 ccatctagca acgtcctctg ctgaaacctt ctcgtcacca aaaagcacta cttcatcccc
     1861 aactattgct cctggaactt ctgtcatatc aaccatcgtc atatccatgc aaacagtacc
     1921 tacaacaggt gcggtaaagc ctcttattga aaccctagca cgatttgaaa gccttctcac
     1981 gtatccgtct gcatagccaa taggaagagt tgcaattatt gatggccttg atgctatgaa
     2041 tgttcctccg tagcttactg aagtccctgc agtaatgcgc tttaactggg taatccttgt
     2101 ttttagcttc attacaggct ttagttcaaa attagtccga tcggcggcgg ggctctcctg
     2161 agccgtgagt cctgagcttg tcgaaagggt cgaacggtcg aagggctcac cacagggtcc
     2221 cattctccca gcgccgtata gcattattcc aggcctaacg agattcatat gagattcagg
     2281 aaatctctga attgccgcgc tatttgccga gtggatgtat ttagggttta accctgcttg
     2341 attaataaga gaaaacatca cattaaaaac cgaaatctgt tttaaagttg gttctctcga
     2401 gtccacatct gcattcgcaa aatgggtgaa aaccccctct aattcaagat ttctacaatt
     2461 taggaggttt tcaagaaaat cacatatctc gctcaaaccc acaccgagtc tgttcatccc
     2521 tgtatcaacc tttaagtgat acctaacctt cttgcctatc ttttctgcat atgtattaat
     2581 ggcatgagca gaagctaagg aaaacaaaga aggggttaaa ttgttcttaa ccatagcctc
     2641 aacctcatct aactgaattc cactgagtaa taatattgga ctatctattc cggaacccct
     2701 aagttcgagg gcttcttcaa ctgttgcaac cccaagcaag ttagaacctg attcgatcaa
     2761 tgcttttgat acattaactg aaccgtgtcc gtaggcatca gcctttacta ctgagatgac
     2821 accaacatcc ttacccacga ttcgtttcac tttgagaaaa tttgacttta gcgaattaat
     2881 ctctatctca gcccaggtag gtcgcataat taagtagggg cgagtctagt gttcgcccag
     2941 aaatagatat aaccctctgt ttactttaag gtcaagatgg ttttaatcca gactgagaaa
     3001 aatacctctt gcatccttag atgtgagatt tgatatggcg gttactttat cgctaaattt
     3061 tttaagggat tccaccgttt cattacccgt taaatcaata agcacggaaa acaccttgaa
     3121 tttgagctta tcttttttat caagaaaaat tttaagccac tcatctctaa catcacactc
     3181 accgtcagta ataaagacaa tgtcgcctct tgtaaacctt gactctttaa gaaactccaa
     3241 ggccttatca agaggatcct caaaatcagt tcctccacct gggaaataac cagcaagttc
     3301 tattatctct tcttctttca ttcgccagtt ctctttcact ccagagccaa aaaccttaag
     3361 ctgagttccc tttgaagaaa acacaatcac agagaatttt cttctctgtc ttcttgcaat
     3421 ctcaagaagg ctcaagcaca ccgcttttgc ccacatttcc ttgtttcctt ccattgaaga
     3481 gcttccgtca acacatacaa taagtggacc cctacccttt tcttctttta gatagtattg
     3541 aagaagtctt ccctcgatta acctttttag aaaatccttt cgtaatgctt tgtgtcttag
     3601 aaatgataac tctgctggaa taattctccc tatgtcattt ccaagcgaaa tgtcatagac
     3661 ttcgcttccc ctttttgacc agatttttct tcttgaagaa aacatctctt ctttaagacc
     3721 tccgaccaag agtgcaagcc ttctaagttt ctcgttttta aatagtttct ccgcaagatc
     3781 gagcttttct ccaataggtt tttcttccgg cattcccatt gaggcacccc actccataag
     3841 ttctttttct acttcctcag tcctctctga tgcctgcttt aaagaagacc gaacgagatt
     3901 ttgaagcttt aaatccatcc gttcgagtct ttctttttgc tctttctgaa ggtctttcag
     3961 ctctccctcc tgagacctta gctcaaactg agtttttttc tttccttctt taagagattt
     4021 attggatgat tttttctttt caatttcttc ccaggtctcg acctcgtctt tgagctcgtc
     4081 taaattttct tcagcttttt ttagttccca ctctttgata agggactttt cagaaagccc
     4141 gtattccgac ttcacccact taactatttc acctcctaac gtcatagtag caatagcgga
     4201 attaaaatca ttgagaattg tctcctctct tagctcttta tgatcttcac ttgagagagc
     4261 cttctcaata agctttctac ccataagcac acttctatta acctcgtctt caggcagagt
     4321 tatgacgtta tacttataga agcttgcaaa taaatccaga atcaaagagc ggaaatcagg
     4381 caagaggtgt gagccacgtt cgataaggtt cttaagttcc tcagatgtag cttcaagttc
     4441 cccaaaaagc tctctatcaa aagaatcggt ttctatattt tgatcagatt tcctattgtc
     4501 cacgctatta aggtaacaca cgttagatat tgcatcaatt caaacccatt gcggtatatc
     4561 ccaaatgaga atattatgga tagagaccga ttgatgatat caacaaagga gtaagaaaat
     4621 caggcagata atttatacct cagagaagat cacaaatttt aatggtggaa attcacctac
     4681 cctcagcaat cagcactttc actctgataa aaagaaaaga taaaacgatt tcagacaaac
     4741 aggagagaca aatggcgcac ataaacaaat ttgaaaatgc tttggatgag ctatttaaga
     4801 agcgtacatc ctggctgcgt tcggctattg gtcggaaaaa acctggtcct cctcctaaaa
     4861 ttacaaggag gtttgtgaaa aacggaattt caaatttaca agaaattgca tcaaatgctt
     4921 taacaaataa gctagccaaa tcagaattta ataagcacgc tcttggaaaa agagcttggc
     4981 attctaaagg ttttggcatt gatgaaaaaa agagtttgtt cgaagaatgg tttaaaaaat
     5041 aatttccaaa attaataaat tgtatttatg ttttttgggg aaatgaaaaa tgtatctatg
     5101 tcgtgagaac aacagtctat ggaggaagcc gtccctcgtc tcattttatc aaattttggt
     5161 ttaaggatgt aaaacgaatt gatattctcc catctgaacc cgcaagtcaa acgccgaaac
     5221 ttgaatgcct tgctattcat cactttgacc cagtacgaaa caagaaaaag ttagcaacaa
     5281 agaaatagat gaaaaaatgt cttttctgtg ccatccataa aaatatagaa gaagagttaa
     5341 gaaaattttt taaactgaaa taggtagcaa taagggcagg ctatagacga gcaaagaaat
     5401 aaaggctttc gaaaaagtta tacagactta aaaaggacga cctttgccta atatgagggt
     5461 atgcgaatct tactccgtgg gatataatac taaagaaagg aggtaaaaaa catgagtgta
     5521 aagaaaatat tgatgttagt tggtgactac gtggaggatt atgaggtgat ggtgcctttt
     5581 caggcactgc tgatggtggg acacactgtc catgcagtct gcccgggtaa aaaagcgggt
     5641 gagaaagtta ggactgctgt ccatgacttt gagggagatc aaacttacag tgaaaagcca
     5701 ggccataact tcgcgttgaa cgctactttc gacgaagtga aagcggaaaa ctatgatgcc
     5761 ctcgtaatcc ccggtgggcg tgcgccggaa tacatccgtc taaacgacaa ggtgttagat
     5821 atcgtgcgtc acttttccaa ggcgaataaa ccaattgcag cgatttgtca tgcggcgcag
     5881 gtattagtag cagccgatgt ggttaagggg agaagttgtt ccgcctacta tgccgtggga
     5941 ccagatataa agcgcgcggg cggtaagttt atgaacatac cggttgacaa agctcatgtg
     6001 gacggcaatc tggttaccgc acctgcctgg ccagcgcacc ctgagtggtt agcaaagttt
     6061 ttaaaaatac tagggaccac gattaagcca tagcaagaaa ggagggcggt ctcaaatttt
     6121 gacattatag aagtaaaaga agatgtagga gaaaaattga agcaaataga agttgaatgt
     6181 tgagactgct ctcttctcgg ttcttcaagg aaagccaatt aagaattcac tctgtctcat
     6241 tattaataag agacactgtg attagataat aaatatggag gtgtaacaac agtacaattc
     6301 agcgtaaatc ccaatcgttt cgacccatac aaacaattca aatttcgtgt caagtgggat
     6361 ggtagataca tcgccggggt gtgcaagatg agttccttaa gaagagtgac agaagttgtc
     6421 actcaccgcg aaggcggtga tccctgtacc gtccataaat cgcctggaca gacctcctat
     6481 gagccagtcg tgcttatgag aggtcgcact cacgatacag agtttgaaaa atgggccaac
     6541 aaggtctgga attatggctc aggccttggt gccgaagcct cactaaagga ttttcgaaag
     6601 gatatcatta tagagcttac aatgaggctg gccaacttgt aatggcattc aaggtctatc
     6661 ggtgctggcc ttctgattat attgctctca gcgatctaga cgctaacgat acctccgtcg
     6721 ccttcgaatc catcaccctt cagcacgagg gctgggagcg cgattactcg gtcacggaac
     6781 ctgcggaacc ttcattcact gagccttaat tatgccctaa aagattgcag aagagtaaac
     6841 tgacaagata agcctcagct tttaaatcag aactcgcttg atcctacaaa atgcatgatt
     6901 atagtgagtg taaatagctt tggaggttat tgaaaatgat gaaaaaagaa ataaagaaag
     6961 tagtaagaga aagatatgcc aaaatagtga aacaggaaag ctcttgttgt ggcactatag
     7021 aatcctcatg tggaagcagt aactccgacc acgaaataag taaggtaata gggtactcag
     7081 aagaagaact taaagctgtt cctgaagatt caaatttagg cctcggctgt ggaaaccccg
     7141 ttgctcttgc ctctttgaga gaaggagaga ctgtccttga tcttggctct ggtgcaggat
     7201 tcgactgctt cctcgcggct aacaaagttg gaaagaatgg acgggttata ggcgttgata
     7261 tgaccccaga aatgattgag aaagcaagag aaaatgcaag aaaaggcaat tacgagaatg
     7321 ttgagtttag gctaggagat attgaaaatc tacccgttgc tgataactct gttgatatag
     7381 taatttcaaa ttgtgttatt gtcctttcac ctaacaaaag aagggttttc tcagaagcgt
     7441 ttagggtcct aaaaccaggt ggtagactta tgatctcgga tatagtgttg ctgaggcagc
     7501 ttccagactt cataaaaaac tccatagagg cttatattgg ctgcgtctct ggtgcaatga
     7561 tcaaaggtga atatataagg gaaataaaag ccgcggggtt tcacgatgtt aaaataatta
     7621 acgagacatc attcgctatt gaccatgtaa ctagcagtca acccgcaagt tcaattattg
     7681 acaaatttaa aataccacct gaaaaagtaa cagaaattgc gagttctatc ttgagtgtaa
     7741 gagtttatgc tacaaaacct ctacaaacgc aataagctca ggagaggctc acgacgaagc
     7801 ccgcccccta catctataca aataaaaacg atggtcgagg ttttccaacc tcgaaaaacg
     7861 gagtatctca tggatgcccg attacaacat tcgggcatga cagaaggctt gtcattccgc
     7921 atatatttag cgggggtcca ttttggtttg tcattcccaa acgtcattat tgggaatcca
     7981 agaaaagtat gaatacccaa aacattcggc catgacaggt tagaagcata ttaggtagga
     8041 gcaccttcaa agcgtgtaaa attcctcctt taaaaaccgg tgactggaaa gtcgcagcta
     8101 tcgaagggat aatcttcacc ccttccatcg ttcgattgat ccgctacaat tatattatta
     8161 tattgagctc actcagaagc aggctgagtt caagcataaa acaaattaaa aggagaatac
     8221 aatgtcaaaa gcaaaatcaa aaaacgataa ggagaagaag aatacaatag ccaatcccag
     8281 agctgatgaa aagaacacaa cgcttaaatt tgaggacata ctatatgaga agaaagaccg
     8341 tatcgcacgt gtaaccatta accgtcctga tatgcgtaat gcacttcggt caaagacgcg
     8401 tgaagagctt gcaattgcga ttgaggatgc gtgggtcgat gacaacattg gtgtcattgt
     8461 gatcacaggt gctggcgata aggcattctg cgcaggtggc gacctttctt ggattacaga
     8521 ccccaagaaa aag
//
LOCUS       DATDRL010000137         3225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_279640, whole genome shotgun sequence.
ACCESSION   DATDRL010000137 DATDRL010000000
VERSION     DATDRL010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279640"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="VI935_04940"
     CDS             complement(<1..1141)
                     /locus_tag="VI935_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HLE24979.1"
                     /translation="MKTTKYSWKKLKWGDLATLEYGKGLRGYQNTEGTYPVYGTNGQI
                     GWHKEPLCHKHGIIIGRKGAYRGIHYSSKPFFVIDTAFYLNPITDSFDVKWAYYQLLD
                     FDINSLDSGSAIPSTSRDAFYQIRVNLPPLSTQRKIASILSAYDDLIENNTRRIQILE
                     EMAQAIYKEWFVNFRFPGHEKVKIVESDLGLIPEGWEINPFLEIAEFVNGFAFKPHHW
                     GNSGKPIVKIAELKSGITEKTPFYSGHDIPTKFHVKNGDVLFSWSADLDVYIWSHGEA
                     CLNQHIFNVLPKRGYTKLFLFYSLKNRMQEFRSKSLGTTMRHIKRSALAQVKFVVPST
                     ELRDLFESYAQPMIQEILNLTSKNNILRRTRDLILPKLISGEIDVE"
     gene            complement(1138..2748)
                     /locus_tag="VI935_04945"
     CDS             complement(1138..2748)
                     /locus_tag="VI935_04945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HLE24980.1"
                     /translation="MQVGLFINEIEKRLWESADQLRANSKLKSSEYSIPVLGFIFLRF
                     ADYKFTIAKDEIEKQHRDVGSRRGITKADYQARGVLYLPENARFSYLLNLPEGENIGK
                     AINDAMRAIEAENEDLKDVLPKTYNRLENDVLVTLLKTFSSIPMDIEGDVFGKIYEYF
                     LGKFAMAEGQRGGEFFTPTSLVKLIVEVIEPYHGRIYDPACGSGGMFVQSAKFVERHK
                     KNPSAEISVYGQEKVAETVRLCKMNLAVHGLSGDIRQGNTYYEDIHNSIDKFDFVMAN
                     PPFNVNGVDKEKIKEKIKGDKRFIYGIPKPDNANYLWIEVFLSALNEKGRAGFVMANS
                     ASDARQSELEIRKKIIEDHLVDVMIAIGSNFFYTVTLPCTLWFFDKGKCRDAINGVPT
                     DRKDKVLFIDARNIYTQIDRAHREFTPEQIEFLANIVKLYRGESVVETQNFASLGDRW
                     LEYFPKGKYRDIPGLCKVATLEEIREQGYSLNPGRYVGVAEKKEEDFDFYERLEEMYE
                     ELEKLNTEAKELEERIAENMANLLGRMR"
BASE COUNT          936 a          696 c          554 g         1039 t
ORIGIN      
        1 cctccacgtc aatctcccca ctaatcagtt taggtagaat aaggtcgcga gtgcgtcgta
       61 gaatgttgtt tttagaagtc agatttaata tttcttgaat catgggttga gcataagatt
      121 cgaataagtc tcttaactcc gttgacggaa ccacaaattt tacttgagcg agtgcagaac
      181 gttttatatg cctcattgtc gttcctagac ttttacttct aaactcctgc atgcgattct
      241 ttaatgaata aaatagaaaa agttttgtat agcctctttt aggcaacaca ttaaaaatat
      301 gttggtttag acatgcttct ccatgggacc aaatataaac atcaaggtca gcagaccatg
      361 aaaatagaac atcaccattt ttaacgtgaa actttgtagg tatatcatga ccactataaa
      421 agggtgtttt ctcagtgata ccacttttaa gttctgcaat tttaactatt ggcttgcctg
      481 aattccccca gtggtgtggt ttaaaggcaa aaccattcac gaattccgca atttctaaaa
      541 aggggtttat ctcccacccc tcaggtatca atcctaaatc tgattcaact atcttcactt
      601 tctcatgtcc tgggaaacgg aaattaacga accattcctt gtatatggct tgtgccattt
      661 cttctaaaat ctgaatacgg cgggtgttgt tttcgatgag atcgtcatag gcggaaagga
      721 ttgaggcgat tttgcgttga gtagagagag gaggcaaatt gacacgtatt tgataaaagg
      781 cgtctctact tgttgaggga atagcagagc cactatccaa gctgtttatg tcaaaatcta
      841 gcaactgata atatgcccac ttaacatcaa atgagtctgt tattgggttt aaatagaaag
      901 ctgtatctat cacgaagaat ggtttgcttg aataatgaat cccacgataa gcacctttgc
      961 gaccgataat aatcccatgc ttatgacata atggctcctt atgccaacca atttgtccat
     1021 ttgtcccata tactggatag gttccttcag tattttgata tcctcgtaaa cctttaccat
     1081 actctagagt cgccaaatcc ccccacttaa gctttttcca agagtatttt gtagttttca
     1141 tctcattctg cctaacagat tagccatgtt ctcggctatc cgctcttcca gttcctttgc
     1201 ttcagtattg agtttttcta attcttcata catttcctcc aatctttcat aaaaatcaaa
     1261 atcctcttct ttcttctcag cgacaccaac atagcgacca gggttgaggg aatagccttg
     1321 ctctctgatt tcttcaagtg ttgccacctt acaaagccct ggaatatcac ggtatttgcc
     1381 tttggggaaa tattccagcc atctatcccc tagagacgca aaattttgcg tctctacaac
     1441 ggattctcct ctatacaatt tcacaatatt tgcaagaaac tctatttgct caggtgtaaa
     1501 ctccctatga gcacggtcta tctgtgtgta aatattacga gcatctataa atagcacttt
     1561 atcctttcta tctgtaggga cgccattaat ggcgtctcta catttaccct tatcaaagaa
     1621 ccataaggtg cagggtaggg tgactgtgta aaagaagttt gaaccgatgg ctatcatcac
     1681 atctacaagg tggtcttcaa tgattttctt tctaatctcc aactccgatt gccttgcatc
     1741 gctggcagag tttgccatga caaagccagc ccttcctttt tcatttaatg cactcaagaa
     1801 aacctcaatc cagagatagt tggcattgtc aggcttaggg ataccataaa taaacctctt
     1861 atcaccctta atcttctctt taatcttctc tttatcaact ccattcacat taaatggagg
     1921 atttgccatt acaaaatcaa atttatcaat cgaattatgt atatcctcgt aataagtatt
     1981 cccctgacga atatccccag ataatccatg gacagctaga ttcatcttac aaagcctcac
     2041 ggtctccgcc accttttcct gaccatatac ggagatctct gcacttggat tctttttgtg
     2101 cctttctaca aactttgcac tctgaacaaa catgccacca gaaccacagg cagggtcata
     2161 aatccttcca tgatagggtt ctataacttc tacgatgagt tttactaatg atgtgggagt
     2221 aaaaaactca ccgcctctct gaccttcagc catagcaaac ttgccgagga agtattcata
     2281 aatcttccca aaaacatcac cttcaatatc catcggaatg ctggaaaagg ttttaagcaa
     2341 ggtaactagg acatcgtttt ccagcctgtt gtatgtctta gggagaacat ccttgaggtc
     2401 ttcattttcc gcctctatag ctctcattgc atcattaatt gctttaccaa tattttcgcc
     2461 ctcaggtaga tttagaagat atgaaaaacg cgcattttct ggaaggtaaa gaacacccct
     2521 tgcctgataa tccgcttttg taatgcctct tcgactaccc acatctcggt gttgcttttc
     2581 tatttcgtct ttggcaatgg tgaatttata gtcagcaaat ctaagaaaaa taaaccctaa
     2641 aacaggaata gaatattcag aggattttag tttagaatta gcccttagct ggtctgctga
     2701 ttcccagagc ctcttctcta tctcatttat aaatagaccc acttgcattt ttatcctcct
     2761 ttaagcccat taaggtaacg gagtcctttg gttttggttc attttctctt atagttccac
     2821 ttatgtgcct acctcaacta ataccattat ttcactaagg ataacccatt gcaacatatc
     2881 tttagacaaa gtgaggaata tcagttcata atctcaccta tcatagggat ctttcactct
     2941 taaagggtca cacaccaaaa ctgagataga aaatgtttcc caaactttaa tcagctatca
     3001 gaatcttaag ataggggcaa tagggcattg gtatgtaagt tatacaatgt aaatctataa
     3061 ctctatggaa taggtagtcg gaggaatagt ttttgttgat aagatgtctt cacttgttat
     3121 ttatacataa aataggttta gtaataaaga tttaaataag aaaaagcctg aatcgggagg
     3181 ggtctttgaa tgaagatgtc aatttctgat atgccggatg atttt
//
LOCUS       DATDRL010000138         6184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2670283, whole genome shotgun sequence.
ACCESSION   DATDRL010000138 DATDRL010000000
VERSION     DATDRL010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2670283"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1058
                     /locus_tag="VI935_04950"
     CDS             240..1058
                     /locus_tag="VI935_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HLE24981.1"
                     /translation="MIRRDEKGGWVLFNQHDHAELSGAIMTFWGNKEFLRPEPYDEVL
                     FAIRGHDNGWKEWDSSPRINPVNQYPMNFIEMTSSDQAYIWRRCFKRHSVEHPYASAL
                     IALHFGKLNEKSLNKNLNNNMAKSLHSEIIDFVSNMLNINASDLNLSSFPKDVQVNLG
                     LVQIGDIISLALCHGWSSNEIKYAPLNYDSSLSTLSLESSDGSNFTIRPYPFAEPLLR
                     FQVRGRKLKQKKFSNDAELRQMLNQTEYETLQFSIRKDNKMQDARYTMQDTGWM"
     gene            1146..2480
                     /locus_tag="VI935_04955"
     CDS             1146..2480
                     /locus_tag="VI935_04955"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HLE24982.1"
                     /translation="MEEKNITYQAFDEIDQPSLDIIHDCVHCGFCLSACPTYLETGNE
                     LDSPRGRIYLMKSALEGRIPLGQSLVKHLDLCLGCLACEPACPSGVRYGKLIEAGRSQ
                     IERRYNRPFFKKFYRSFLFSIFPYPKRLKFLLPFLLLYQATGINRLIRSSRILHSLFK
                     KLVHTEEMLPNIKFSSIFSSLPEVIPAKGKKRYRVALLTGCVQSVLFSETNAATVKVL
                     SQNGCEVIIPKNQGCCGALSLHSGRLSEARDFARSNIDIFEKLDVDAVIVNSAGCGST
                     MKEYGELLENDTSYSRRAEGLSKKTKDVMEFLANIGLQGELKELKLRVTFQDACHIAH
                     AQRIKAHPRKVILQIPGIEFVELPESDLCCGSAGIYNLIEPEMADRLLERKISKLKET
                     GAQVLVAGNPGCLLQIGMGIKRHGLSIKTAHPVELLDWAYQGTISKPSVNMK"
     gene            complement(2740..3600)
                     /locus_tag="VI935_04960"
     CDS             complement(2740..3600)
                     /locus_tag="VI935_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24983.1"
                     /translation="MDIRTLKLSVVFIILIFTFSYCGGGTGGDAQKASIKLFLEFPEE
                     LFSDSSTELSGEPVLRQNGLEGTVTIEIDVGNFHDEETFDVSDLPIQRTFLVPAKPDV
                     SFICKFITADGLLGAAGSTIADIVFGAENNVFCQLGVLTCLACDNPLCEGERCDPDDE
                     AKICVDGECVTPTPPPTPTPTPTPIPTPTPTPRPIFGCCDLGLACENITNVECAEMQG
                     LFLPGGFCISGGCRTTPPTPTPPPFQVCCEFSEDCAEITNVQCAMLGGAAFFPASCYN
                     CDGSCVFVCD"
     gene            complement(3614..4402)
                     /locus_tag="VI935_04965"
     CDS             complement(3614..4402)
                     /locus_tag="VI935_04965"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLE24984.1"
                     /translation="MKIRILKIFVLLLSVFWVTNSYAQEPEPIGIVTAVKGEVVVVSA
                     GKSQVVEKGTSVFLGDRFETKEASGVKILFDDDTLISLGANANFDITEFVYTPKERKS
                     YSNIFKGKLKAIVQKFERGDSNVEFATPNAVAGIKGTTEFIDADGDICMDDGVSFIRR
                     KDDAWEFTLEDGLCINTLERVPIAKPFDRELREKFESETEVKEEVPSDEEFEEFPEEE
                     LPSEADLTPFGAPGIPPILQDPADILLNGDQTPVDVVIPPPQGP"
     gene            complement(4424..5926)
                     /locus_tag="VI935_04970"
     CDS             complement(4424..5926)
                     /locus_tag="VI935_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE24985.1"
                     /translation="MGYRLRFLVFLIVCAISQPVLADFELNLNKGLALYLKKDYQGAV
                     KFLEQAVVENPNSPVANHILGLSLLKLKKYSESVTYLEKARSLDPKIKRIYLDLGTAY
                     LRIEDYNKAQVELMEAIRQEPDSGLAYYYLGYAQYKLGNHKDAAASFDKAYKLDPDLT
                     LQSRFYAGLSHYKMSNYEQAKKEFEFVGEFGAGTDTAVAALQYLDIIAGLTKRYYGGV
                     STGFQYDTNVAVEADNIDIVSDKSAPRAVFFFNIGYNPYLKPDAVLGGNFSFYMNFNK
                     DLEDFNIQDYRINLYGAKKTQLGGTPVSFFLDYFYDIVLIDGTPASHLFSQSQSVSPQ
                     VSFQWTPYTSTVISYEFRYNNFEDFPERDAVNNNFTIAEVFSICNGRLLLKPGFNFEV
                     NSAKDVANTFNFDYVSPEGFLEALAFLPFEITAFLEFNYYRQDYYNDDFNRVDNQFSV
                     EVAVSKRLYKILYLDLGYVHVSNFSDTDFPPPNPFEYDRDIFSVTLTAKF"
BASE COUNT         1902 a         1295 c         1211 g         1776 t
ORIGIN      
        1 attccctctc cttctttaaa aatcccaaga aaaaaattct aaatataaat gaacaaccct
       61 gctgcaatca catattcaaa accataatgg ataggcggga ctttctagtc ccgctttctt
      121 agacggggtt ggaaaacccc gtctatcgct gtgttatgtt ctgcagagga aatcgtcaag
      181 ttaaccaata cttgcagaac tagggttgct tagctaggat atgattaagc gtataattga
      241 tgataagaag agacgaaaag ggaggttggg tcctttttaa ccagcacgac catgcagagc
      301 tttctggggc gattatgacg ttttggggaa ataaagaatt tttgaggcca gaaccttatg
      361 atgaggttct atttgcaatc cgggggcatg acaatggttg gaaagaatgg gattcttcac
      421 caaggataaa tcctgtaaat caatacccaa tgaacttcat agagatgacc tcatctgatc
      481 aggcttatat atggaggcgt tgctttaaaa gacattcagt agaacatccc tatgcctcag
      541 cacttatcgc acttcatttt ggaaaactca atgagaaatc tctaaataag aatttgaata
      601 ataatatggc aaagtcatta cacagcgaga ttattgattt cgtctctaac atgttaaata
      661 ttaacgcatc agatttaaac ctgagttctt ttcctaaaga tgttcaggtc aatctagggc
      721 ttgtccagat tggagatatc atctcgcttg cactttgtca tggctggtca tcgaatgaaa
      781 ttaaatatgc accgcttaac tacgatagct ctcttagtac gctcagcctt gaatcaagtg
      841 acgggagtaa ttttacaatt agaccttatc catttgctga gccattgctt agatttcagg
      901 ttcgtggacg aaaactaaaa cagaaaaaat tctcgaacga tgctgagctt aggcaaatgt
      961 taaaccagac tgaatatgaa acgctccaat tttcaattcg aaaagataac aagatgcagg
     1021 atgcaagata cacgatgcag gatacaggat ggatgtaatt ttcatgcatc atgaatcgtg
     1081 tatcatgtat cgtttaatag cgtaaattcg tgttcattag tggccaaata gttataaata
     1141 atcaaatgga agagaaaaac ataacctacc aggcctttga tgaaatagac cagccaagtc
     1201 tagacattat tcatgactgt gtccactgtg gattttgcct ttcagcttgt cccacttatc
     1261 ttgagacagg aaatgagctt gattccccga ggggaagaat ctacctgatg aaatcagcct
     1321 tagaagggag aattccactc ggtcaatcgt tggttaaaca tttggactta tgtcttggat
     1381 gtctcgcctg cgagcctgct tgtccgtcag gggtgcgtta cggtaagctc attgaggcag
     1441 gaagatccca gattgaacgt cgttacaata gacccttttt taagaaattt taccgatcat
     1501 tcttgttctc aatttttcct tatcctaaaa gactcaagtt tctccttcct tttcttcttt
     1561 tgtaccaggc aactgggatt aataggctaa tcaggtcttc gagaattcta catagtcttt
     1621 tcaaaaagtt agtacatacg gaagagatgc ttccgaacat aaagttttca tctatctttt
     1681 catccttacc tgaggtaatt ccagcaaaag gaaaaaaacg ctatcgggtt gcattgctta
     1741 ctggatgtgt ccagagtgtt ctattctctg aaacgaatgc agctactgtc aaggttctct
     1801 ctcaaaatgg atgcgaggtt atcattccga aaaaccaagg ttgttgcgga gcgctatctt
     1861 tacactcagg acgcttgtcc gaggcaaggg actttgcgcg ttccaacatt gacatttttg
     1921 aaaagctcga tgttgatgca gtcattgtta actcagcagg atgtggatct acaatgaaag
     1981 agtatggaga gttgctagag aacgatacat cctactctag gcgtgcagaa ggactaagca
     2041 aaaaaacaaa agatgtaatg gagttcctag caaacattgg acttcaagga gaattaaaag
     2101 aactaaagtt aagggttaca tttcaggatg cctgtcacat tgcccatgct cagagaataa
     2161 aagcgcatcc aagaaaagta atactgcaga ttcctggaat cgagtttgtt gaacttcccg
     2221 aatccgacct ctgctgtgga agcgccggga tttataacct tattgaacct gagatggcag
     2281 ataggctcct tgaacgaaaa atctctaaac tcaaagaaac aggagcacag gttttagttg
     2341 cagggaaccc tggctgcctg ctacagatag ggatgggaat taaacgtcat ggattaagca
     2401 tcaaaaccgc ccatcctgtt gaactattag attgggcgta tcaaggaact atttctaagc
     2461 cttcagttaa catgaaatga ggaaactacc tgaaggtgac aaataatgca gcaattctgg
     2521 cttgtggttg gatcaggaag aaactggaaa gtatcttttg aaaacagcaa ccctatatgt
     2581 cacaaacttt ttcttatttc aaacacagta agtcgagtta gctgtacctt ggacaatcct
     2641 catttaataa aattttcgat aagctttgta agaatgctcc cgacgtaata tataaactga
     2701 aataacagcc catccagttg agctacttga ttgggcatat taatcacaaa caaaaacgca
     2761 actgccatca caattatagc aagatgcagg gaagaaagcg gcacctccta acattgcaca
     2821 ctgtacgttt gttatctcag cgcagtcctc gctaaattcg cagcagactt gaaatggggg
     2881 aggagtcggt gtgggtggcg tcgttctaca ccctccacta atgcaaaaac ccccgggtaa
     2941 gaataaacct tgcatctcag cgcattccac atttgtaata ttctcgcatg ctagtcctaa
     3001 atcacagcag ccaaatatcg gtcttggagt cggcgtagga gttggaatgg gtgtcggtgt
     3061 gggtgtaggc gttggtggcg gtgtgggcgt tacgcattca ccgtcaacgc atattttagc
     3121 ctcatcatca gggtcgcatc tctctccctc gcatagcgga ttatcacagg caaggcatgt
     3181 cagtacaccg agttgacaaa atacattatt ttctgctcca aaaacaatat ctgcaatggt
     3241 gctacccgct gcccccagta aaccgtctgc agtgataaac ttgcatatga atgatacatc
     3301 aggcttcgcc ggaaccaaaa atgttctctg tataggaaga tcggagacgt caaatgtttc
     3361 ttcatcatgg aaattaccta catctatctc aatggtgaca gtaccttcta gcccattttg
     3421 tcttaaaacc ggctcaccgg ataattctgt ggacgaatca gaaaacaatt cttcaggaaa
     3481 ttctaaaaaa agtttaatag aggctttttg tgcgtcccca cctgtaccac ctccacaata
     3541 ggaaaaagta aaaattagga ttataaaaac aacactaagc tttaaggttc ttatatccat
     3601 tttctatccc tctctaagga ccttgcggtg gtggtatcac aacatctaca ggtgtttgat
     3661 cgccgttaag caaaatatca gcggggtctt gaagaattgg cggaattcca ggcgcaccaa
     3721 aaggcgtcaa atcagcttca gaaggcagct cttcctctgg aaactcctca aattcctcat
     3781 cgctagggac ttcttcttta acctctgttt cactttcaaa tttttccctt aattcgcggt
     3841 caaaaggctt tgcaatgggt accctttcta aggtattaat acatagccca tcctccagcg
     3901 taaactccca cgcatcatct tttcttctta taaaactcac accatcatcc atacagatgt
     3961 ccccatcggc atctatgaac tctgtagttc cttttatacc tgcgacagcg ttaggggtgg
     4021 caaattcaac gtttgaatcc ccgcgttcaa acttctgaac tattgctttg agtttcccct
     4081 tgaaaatatt ggagtaggat tttctctctt tcggtgtata aacaaactct gtaatatcga
     4141 aattggcatt tgcccctaaa gaaatcaagg tgtcatcatc aaatagtatt tttacacctg
     4201 aagcctcttt tgtttcaaac ctatccccta gaaatacact agttcccttt tctaccacct
     4261 ggcttttacc agcgctcacc actacaacct cccctttaac tgccgtaaca atacctatag
     4321 gctcaggctc ttgagcatat gaatttgtaa cccaaaacac cgataataac agtacaaaga
     4381 tttttaatat cctaatcttc atgtagttgt ccgtggaaaa cccctaaaac ttcgcagtaa
     4441 gtgtaacaga aaatatatcc ctatcatatt caaatgggtt aggtggtggg aaatctgtat
     4501 ccgagaagtt tgaaacatgc acatatccaa gatctagata taaaatcttg taaagcctct
     4561 ttgatacagc aacctctacg ctaaattggt tgtcaaccct gttaaaatca tcattataat
     4621 agtcctgtct ataatagtta aattcaagga aggcagtaat ttcaaaggga agaaaagcga
     4681 gcgcttcaag aaagccctca ggggaaacat aatcaaagtt aaaggtatta gctacatctt
     4741 tagcgctatt tacctcaaag ttgaatccag gctttaaaag aagccttccg ttgcaaatgc
     4801 taaatacctc agcaatagta aagttattat ttacagcatc cctctctggg aaatcctcga
     4861 aattattgta tctaaactcg taggaaatta ccgtgctcgt atagggggtc cactggaaag
     4921 atacctgtgg gcttactgat tgtgattggg agaagagatg actagcaggt gtgccatcta
     4981 ttagtacaat gtcgtagaaa tagtccagga aaaaactcac cggggttcca cccaattggg
     5041 tcttttttgc tccgtataga ttgattctgt aatcttgaat attgaaatcc tctaaatcct
     5101 tattgaagtt catgtaaaag gaaaaattcc cacctagaac ggcatcaggt ttaagatagg
     5161 gattatagcc aatattgaag aagaaaacgg ctcggggagc gcttttatcg ctgactatgt
     5221 caatgttatc agcctctaca gcaacgtttg tatcatattg aaaacctgtg ctaaccccac
     5281 cataatacct cttggtaagc cctgcaatta tgtctaaata ttgaagcgcg gctacagcgg
     5341 tgtctgtccc tgctccaaac tcccctacaa actcaaactc tttctttgct tgctcgtagt
     5401 tactcatctt ataatggcta agaccagcat aaaaacgaga ttgaagcgtt aggtcaggat
     5461 cgagtttata agccttatca aatgaagcgg ccgcatcttt atgattccct agcttatatt
     5521 gagcgtaccc taagtaataa taagcgagac cgctgtctgg ctcttgcctt atagcctcca
     5581 ttaattcaac ttgagcctta ttgtagtcct ctatcctgag atacgcagtt cctaaatcaa
     5641 gatatatccg ttttattttc ggatcgaggc ttcttgcctt ttctaggtag gtaacggatt
     5701 cggagtattt ctttagctta agaagggaaa gcccaagtat atggttggct acagggctat
     5761 tagggttttc aactacagcc tgctcaagga atttaaccgc cccttgataa tcttttttca
     5821 gatatagagc aagtccctta ttgagattaa gttcgaaatc agctaaaaca ggttgagaga
     5881 tagcacatac tattaagaaa acgagaaatc tcaatctgta tcccaatcct ccgtctcctt
     5941 taagccgtag ataactttga ctgcaaaatt ttgaacagac ttactaaaaa aacaatgtga
     6001 ctttactacc tttaaaaagt gttgtcaaga agtattttga catatgtcat ttaaaaatta
     6061 aaagtgaacc gtttttacac gatttaaaga ttaaaagtga acaccctaag aagtcgtaat
     6121 ctaaagccaa aaaggaggct tttagattat ggcacaacta cacaagaaat tcaccgatag
     6181 tcaa
//
LOCUS       DATDRL010000139        21478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3462504, whole genome shotgun sequence.
ACCESSION   DATDRL010000139 DATDRL010000000
VERSION     DATDRL010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 21478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21478
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3462504"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="VI935_04975"
     CDS             <1..166
                     /locus_tag="VI935_04975"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HLE24986.1"
                     /translation="PSGKSVELKPSGSHIMLIGLNKSLKAGDKIELDLKFEKFGIIKA
                     RAQVKGMQSH"
     gene            271..1791
                     /locus_tag="VI935_04980"
     CDS             271..1791
                     /locus_tag="VI935_04980"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HLE24987.1"
                     /translation="METMSYSNFISYIFDTKTFVEGVVENFISRKSQSKNTLIAFIFA
                     SMFLTFACNNEGGGMGETFRNPPEISSSNGVLHATFRLDFSNIKVAGQKVTTRIYNGR
                     YVPPTLRVRPGDVIRVKLRNFIDEMTNIHYHGMNVSPLGDSDNIFNMIVPTQTFDYEV
                     QIPENHPEGLFYYHSHAMGLSEFQVFSGESGGLIVEGILDPFPQLQGIKQRVMLLKDI
                     QINPDGTVPMDIDSNAPTNRTINGLVNPTIKIRPGETQFWRIGNIGADIYYRLKLDGH
                     ILYEIARDGNRHNQLVPMKEIFLPTSSRVEVLVQGGPKGVYEFRTLPINMGPQGDQYP
                     EVTLATLISEGPEENPIALPTKSEFPQIEDLCNKPVNKKRKFVFSENADGTTFFINDE
                     QFNPDVINTTVQLGDIEEWTIQNISEEFHVFHIHQLDFQVCEENGVKLDFIGRQDTVN
                     LPFMTNGVPGEVKIAIPFTDPVILGKFVYHCHIMAHEDNGMMAVIEVCDDGFCPGE"
     gene            1904..3193
                     /gene="hisD"
                     /locus_tag="VI935_04985"
     CDS             1904..3193
                     /gene="hisD"
                     /locus_tag="VI935_04985"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HLE24988.1"
                     /translation="MKIIDLRKGVFERRLKGLSREVSFSYPRIESDVRKIVEAVRKDG
                     DKALFRYTKKFDKVELTLKTVKVSNKEIETAEALVPEEVKSNLALAAERIKDFHERKL
                     PQEHTFTDSLGNELGWIIRPIEKVGVYVPGGKAAYPSTVLMTVIPAKVAGVKEVFLAT
                     PCPNGQINPAVLVAAYMAGVSAVYKVGGAQAIAALAFGTKSIPKVDKIVGPGNIYVSI
                     AKKLVFGNVDIDMIAGPSEVLVISDGSVPAEWVASDLLAQAEHDEMAVAVLVTNSLEF
                     AKQVKQEVIKRLKKLERKHIAQVSIRNQGRIFVVNTLDDAISVSNAIAPEHLELCVED
                     PKAILPKVKHAAAIFLGSLSTEAFGDYIAGPSHVLPTSGAARFSSPLSVYEFLRMPSI
                     ISVSQGGFKELGDSVINLAYSEGLEAHALSVKVRLDR"
     gene            3433..4839
                     /gene="hisC"
                     /locus_tag="VI935_04990"
     CDS             3433..4839
                     /gene="hisC"
                     /locus_tag="VI935_04990"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HLE24989.1"
                     /translation="MEIRCSFCEITSKEATGIVQRGEHSICSECLKLVKEILDEFSMG
                     DVYEARGTCSFCGREHGGGRMVFQGKNARICNECINEIEEEIGEMKNETSPRKTEKMG
                     NEGQKIQEEPKTLSLLKFRVKKGILELSPYSVPHYECPVKLDGNESPFLLPNEVLEIV
                     ISEIRKIPINRYPDPEARSVREKISKLTGFSLDGIAIGNGSDELIGTIMATFSGGTRR
                     VLYPVPTFSMYRITGTAFGLELFEVELDDRFDIDISETIKEIKKKNPDLIFLASPNNP
                     TGNRFSDTSIFEILDQSKGIVVLDEAYCDFAGRTFLNLIQKYENLIVLRSMSKVGFAG
                     IRMGILFGRSEIVVEINKVRLPYNVNSLTQRTAEVIFDNVGFVSENVQLITRERDRVF
                     KVLKLISSIEAFPSDANFILFRVNDADRIFKELLRKGILIRNFNSPGRLENCLRVTIG
                     TPEENNIFLVALGEIFSS"
     gene            complement(4911..6473)
                     /gene="lnt"
                     /locus_tag="VI935_04995"
     CDS             complement(4911..6473)
                     /gene="lnt"
                     /locus_tag="VI935_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HLE24990.1"
                     /translation="MKITKYDLILCVISGVLFPVAFVIPYAGIISWFLIIPFFWAIEN
                     KSPWNAFRLGVLTGTIANAIGTYWLIGTLSRFGGFPDVVSFIFHLAISVYSGFYFAIF
                     AYITTKLELFRKQGLLPALLIASIWTSIEFLFPFLFPYGITNSQADYLPLIQIFDLFG
                     MYSLSFLIIFVNVTLLKVLKKLLEHNITPTLEILTSLILIILIISYGLWKIDLETKKI
                     AEAPKIKVGIVQANFDILEKKQKNESTITERHKLMSHVLHSPELIIWPETAIQAWIPA
                     SQDFLINEGEIVIPQTKNSYFLVGGLSYKFNNEFSPEFSFDQNIDKFNTAFLTDPQGK
                     ILGRYNKIKLLLFGEYLPFTNYIPALKKISPASGDFTPGSDLEILEIKEKGIKIAPLI
                     CYEDIIPSFSRKFVENGANLLVNITNDAWFGKSFEPYQHLTFSIPRAVETRRFLARAT
                     NTGISAIIDSVGRVTARTGIFEQTTLEGTIAIVDGKKTLYTKIGDVFPWGCLIFWIGF
                     VLVAKLRGKINR"
     gene            6622..7101
                     /locus_tag="VI935_05000"
     CDS             6622..7101
                     /locus_tag="VI935_05000"
                     /inference="COORDINATES: protein motif:HMM:NF019542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24991.1"
                     /translation="MRTRKAKEGEKICLLKATLKKFSGRVKVSKTFPHRVLAIPQGLT
                     LYKLAEAIVDSFDFYFDHCFGFYDNIKEWIDSVEGYELFADIGEESEFKGVERTKVKK
                     VFDKIEKKMLFLFDYGDNWHFIVELKGIESSKEGEKYPFVTESSGDAPIQYGPSDDF"
     gene            complement(7087..7842)
                     /gene="lgt"
                     /locus_tag="VI935_05005"
     CDS             complement(7087..7842)
                     /gene="lgt"
                     /locus_tag="VI935_05005"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HLE24992.1"
                     /translation="MYPELFRIGNFTISSYGVMVAFAFLAAYWLSSLEFKRRGLDDRL
                     LGNFLIAAMVGGIVGAKIVYLLENVTFSEFAGNPIHYLLARGGLTYLGGLLGAALLMW
                     YIAYRNKMSFWALGDAVTPGLAIAYAIARVGCLLVGDDYGVPSDLPWAMAFPKGLPPT
                     TERVHPTQIYEILLMGAVFIYLWKIRKKQAPTGWLFSIFLILAGLERFLVEFVRATTP
                     SPIPHLSLAQLMSIGMIIIGIIKLILIHRLKVI"
     gene            complement(7899..8066)
                     /locus_tag="VI935_05010"
     CDS             complement(7899..8066)
                     /locus_tag="VI935_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE24993.1"
                     /translation="MNHLFTIFIVQVIFWILLFILICYFVKKNAELKKEIKLLKDAET
                     YNSNRLTEHSR"
     gene            8573..9169
                     /locus_tag="VI935_05015"
     CDS             8573..9169
                     /locus_tag="VI935_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HLE24994.1"
                     /translation="MLTSPVLVLNRYFFPITVTSVKRAFVMLYCGAAKAVAGNYQTFD
                     FESWAEISALREGDAIKTVSRVIRVPRVILLLRYDHMPRKEAKFNRINIFRRDGDSCQ
                     YCGRKFSRSELTLDHIIPRSLGGRSVWENVICCCMNCNRKKGGRTPDAAKMKLINKPK
                     KPAWSPLTNFSIRAVKYKEWEPFLNFVDVSYWNVELEE"
     gene            9464..10735
                     /gene="tolB"
                     /locus_tag="VI935_05020"
     CDS             9464..10735
                     /gene="tolB"
                     /locus_tag="VI935_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HLE24995.1"
                     /translation="MRYKILIIFLTIAIVFSTDIFARIIIPPIHGPIIKKISVFVPEL
                     SNVSAGGTDGKAREFINVLRNDLLNAGFFEVYDASGVSTDGSGNINFQAFFDAGAEAL
                     VKGEYQSSGDRINIAIRLFDVVQERELLGRSYEANSGRVREAAHRFANAVMKELTGID
                     GFFTSKIVFVSGTGQKRDLYIMDYDGHNTKRLTSHSSIILSPDCSSDGTKVVFNSDKV
                     WSQDIYVITLSPRVEEKRITRGLKLEQSPAWSPDGRKIAYSGNGEIFLANADGTGAVN
                     LTRHNAIDVSPSWSPDGRKIAFVSDRNGSPQIYVMNSNGSNIRKITSGGYNTDPAWSP
                     NLSVNRISFVRVEGNEANIFTVNPDGSDSQKLTSGSRRNENPAWSPDGHYITFGSSRD
                     GAKNIYIMYLNGENQRSLTKGGGKSFPTWCR"
     gene            10987..11496
                     /locus_tag="VI935_05025"
     CDS             10987..11496
                     /locus_tag="VI935_05025"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HLE24996.1"
                     /translation="MKVFLSEAAFMGLVLSSIEVYKKECLGLLLGYKLEDRYIVEYSV
                     AYQSAKRMRNGVSPNRLRHSRIEGVLPKFDKLELLGDFHSHPQYGSLKGIPEPSSLDI
                     NDMKQGKLYMIVAINDNEHSVRWRENQDGTISGTLNDFHFKISGYNFEENKTRRIPIR
                     CPFCLVIEN"
     gene            11530..12078
                     /gene="thpR"
                     /locus_tag="VI935_05030"
     CDS             11530..12078
                     /gene="thpR"
                     /locus_tag="VI935_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HLE24997.1"
                     /translation="MRVFIAAIIPDEIQIEIDRYLKEIKPNWEGVKWEKYNKLHVTLK
                     FLGEVEESSVEKIKSVLGELVLPCSSFEMTIVRFGGFPYLKNPRVLFIGLSENPELLK
                     LHGEIEERLEVLGFKKENRRFVPHVTVGRIKGRSKSRGTFPAPNNTHFFISQISVMKS
                     ELKPEGSKYTPLSVFRLKDRST"
     gene            12216..12530
                     /gene="rplU"
                     /locus_tag="VI935_05035"
     CDS             12216..12530
                     /gene="rplU"
                     /locus_tag="VI935_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HLE24998.1"
                     /translation="MQAVIKTGGKQYIVKPGDIIKVEKIEGKPGDSVEFNEVLMVTDS
                     QKGNKLGLPLLKDFSIKGEILDHVKGEKITVFKFKRKKGYRRKKGHRQVYTSIRITEV
                     NG"
     gene            12543..12803
                     /gene="rpmA"
                     /locus_tag="VI935_05040"
     CDS             12543..12803
                     /gene="rpmA"
                     /locus_tag="VI935_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HLE24999.1"
                     /translation="MAHKKGGGSSRNGRDSEGRRLGIKRFGGEFVKAGNILARQRGTK
                     FHPGENVGLGRDFTLFSLVDGRVKFERMGKDKTKVSVEPHSN"
     gene            13015..14247
                     /locus_tag="VI935_05045"
     CDS             13015..14247
                     /locus_tag="VI935_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25000.1"
                     /translation="MRRHNIVFFGSRAKSCIVIALIFIVLSITVSVFNSLFNYFQFFG
                     YQAKEVEKLFDGRKDFSMLAKDLLNTRSDIAYVKLLDKNGIMIDSLGRESFIDVKKHS
                     FLTPDKNTVIVGIKEMKNKKFVLHSLLWSIAIGIFFSAITIFAILILYNKNQNHHVER
                     VITAIKRISRGDLSARLDIEKGLGNDLLMIRLFESFNQMVDQLRKKEEATKETHRFQP
                     TVIISEKKEEAKIRRVIAFVAKVSDFENLSSRLSTSEFSSFLTEYRKAASSIVSDYGG
                     VIEALLQEEIVALFNVPDEKDKPELRAVCAAVEVLQVLANMSRKRMIEGKEAINGKIG
                     IGAKAVPFYVESGIPQGVKEVVALAKGICQDAPLWKVLVSSEVYKFVSDYVDAKEIDV
                     EHGTSFSIVAVEEGVIQV"
     gene            14476..15555
                     /gene="rlmN"
                     /locus_tag="VI935_05050"
     CDS             14476..15555
                     /gene="rlmN"
                     /locus_tag="VI935_05050"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HLE25001.1"
                     /translation="MNKINIKDLGPEELEYFVLNLGEKSYRATQLGKWIYKRGVNSFD
                     KMTDLSKDFRASLSQAATISSLKMIEERISFDGTKKFIFGLFDGNRIESVLIPENGRN
                     TLCISTQVGCALGCTFCLTGRVGRIRDLKTSEILDQFLEVNNRNNRSITNIVFMGMGE
                     PLDNLENTVKALKSFTHPDFIGFSPKRITVSTSGIVPKIRELGKQVSVNLSVSLNAPT
                     DELRNEIMPINKKYPIMELIEESVRFPVPLRKTLTFEYVLINEVNDSDKDALELVELL
                     KGVRCKVNLIQFNEAYPLPYKSPPDERVLGFQKVLFSYGLHVRIRKNRGRDILGACGQ
                     LAADYPLRNRKANNFQPRQNNFKSV"
     gene            15756..18395
                     /gene="alaS"
                     /locus_tag="VI935_05055"
     CDS             15756..18395
                     /gene="alaS"
                     /locus_tag="VI935_05055"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HLE25002.1"
                     /translation="MKASDIRKTFLKYFEENGHTIVSSSSLIPKKDPTLLFTNAGMVQ
                     FKDVFLGVDKRPYTRATSCQKCVRAGGKHNDLEIVGHTGRHHTFFEMLGNFSFGDYFK
                     DEAINFGWELLTEIFKLPKDRLWVTIFRDDDEAFHIWKAEIGLPSERIVRMGEMDNFW
                     AMGDRGPCGPCSEIIIDQGEAFSCGEPSCAVGCDCDRYLELWNLVFMQYERDSLGNLT
                     PLPKPSIDTGMGLERITAVLQGVKSNYETDLLRGLISSVEERTGKLYGESLSLDVSMR
                     VIADHSRAVTFLISDGVFPSNEGRGYVLRRILRRAVRHSRALGIKEPFLFSLTDQVWS
                     IMGEAYPELEERLSFVREVVKNEEERFFETIDRGLELLNLEVEKHKGEKILPGDVAFK
                     LYDTYGFPIDLTEDIARESGLIVDYPEFEKEMEKQRERSRQAWKGSGEEELVPLYKEF
                     TSGGFIVNFTGYEKTKDTGRITAIIKDGRLVDSASEGVRVELITDKTPFYGESGGQVG
                     DRGVIEGESGYAEVLDTKKPLLTLIVHQVSIRKGTLWVGDNVELRADERHRYGAKTHH
                     TTTHMLHSVLRETLGTHVRQAGSLVAPERLRFDFTHFSSIDDGTIRRIEDIINDRVRW
                     DDRVLTQVDIPYEEAIKTGATAIFEEKYGDRVRIVSIGDYSKELCGGTHLHTTGEVGL
                     FKLIGETASSAGVRRIEALAGESAWRFIRKQEETLIEASSILRVSQSDLISRLKRMVE
                     ENDSLKKELESFKDKVMGEKSKSILDNVKEIKGIKVLSAQVSDVGPEELRKIWDEIRE
                     KLKPGLAVLGSGSDGKAYILVGVTKDLSKRFHAGNIVKELAIIIDGGGGGKPDMAQAG
                     GNRPEKLEEALRKIFEIVNRTLQ"
     gene            complement(18397..19308)
                     /gene="rpoH"
                     /locus_tag="VI935_05060"
     CDS             complement(18397..19308)
                     /gene="rpoH"
                     /locus_tag="VI935_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HLE25003.1"
                     /translation="MAEVKKDVEKKEEYLNKSLPAPTTSMERYLAEISNYSVLSREEE
                     YKLAMRYRKKRDLDSARKLITSNLRFVVKIANEYRNYGLNPMDLIQEGNIGLMQAVKR
                     FDPTKGYRLISYAVWWIRAYVQNYIIKSWSLVKVGTTQAQRKLFYKLRSEKQKMDIAN
                     ETLSPEDYKNLAKELGVSDQAVIEMDQRMGSKDLSLDTEIKGDGEITHIDFLVDDASN
                     QEELVTRAQEEDEVKRELETALKSLKERERFVIENRILKDSPLTLEELGKKLNLSRER
                     VRQIENTALKKIKMVLEKRGVTQGQSD"
     gene            19754..20155
                     /locus_tag="VI935_05065"
     CDS             19754..20155
                     /locus_tag="VI935_05065"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HLE25004.1"
                     /translation="MSSLFFKKEAFKGGIEGEIKKGVLLFNEGFYFECHEFLEEVWRK
                     EKGREKEFLKGLIHVAVAFYHLEYENYKGTINYLRRSYKRLKEFEPLFMGVDIKNFLA
                     ELESYLKNLEKSGKHNLKFLKNAIPRIRLIE"
     gene            20484..20559
                     /locus_tag="VI935_05070"
     tRNA            20484..20559
                     /locus_tag="VI935_05070"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20517..20519,aa:Asn,seq:gtt)
     gene            complement(20788..21240)
                     /locus_tag="VI935_05075"
     CDS             complement(20788..21240)
                     /locus_tag="VI935_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25005.1"
                     /translation="MKTIAISGLILFTLFNLAGLSEETETPNTITMAQHPEWYIKITD
                     WSFYATRGVGIIHHVTIENTSDIAYKNVEVRVHYYSTSTSNYGTEIGNETGVLNVVLA
                     PHSKKTYLEGGAVFGAGSSLFNADNIEVLGALPATGAYDKGIYPKLTL"
BASE COUNT         6698 a         3702 c         4710 g         6368 t
ORIGIN      
        1 accttctggt aaaagcgtcg aattaaagcc aagtggctct cacatcatgc tgataggact
       61 taataaatca ctaaaggctg gagataaaat agaactcgat ctcaaatttg agaaattcgg
      121 tattataaag gctcgagccc aggtaaaagg gatgcagagt cactagatac agcaagattt
      181 aagaaatttt tcttttggct gataatattt ttttgatata ctcacccttt atataataag
      241 cgatagaggt tgtacataac tacaaggagg atggaaacca tgtcttacag taacttcata
      301 tcgtatatat tcgatactaa aacctttgta gaaggagtcg ttgagaactt tatttctcgg
      361 aaaagccagt caaaaaacac attgatagca tttatctttg catcgatgtt cctaaccttc
      421 gcctgcaaca atgagggagg cggaatggga gagacattta gaaatccacc tgaaatctca
      481 agctcaaacg gggttcttca tgcaaccttt aggttagatt tttcaaacat aaaggttgca
      541 ggtcaaaagg ttactacaag gatatataat gggagatatg tccctccgac gctcagggtt
      601 cgtccggggg acgtaatcag ggtcaagctc aggaatttta ttgatgagat gacaaacatc
      661 cattaccatg gaatgaacgt ctcacccctg ggggattcgg ataacatctt caatatgatc
      721 gtacctacac aaacgttcga ctacgaggtc caaattcctg aaaaccatcc ggagggactt
      781 ttctattacc attcgcatgc tatgggtttg tctgagttcc aggtctttag tggggagtcg
      841 gggggattga ttgtagaggg aatacttgat cccttccccc aacttcaggg cataaagcaa
      901 agggtaatgc tcctaaaaga cattcagata aaccccgatg gtactgtccc aatggatatt
      961 gacagtaacg ctcccactaa tcgtacaatc aacggattgg tgaatccaac aatcaagatt
     1021 cgaccgggag agacgcagtt ctggagaatc ggaaatattg gtgcagatat atactacaga
     1081 ttgaaactcg acggacacat tctatatgag attgcaagag atggtaacag gcataaccaa
     1141 ctagtaccaa tgaaagaaat atttctccct acaagttccc gtgtcgaggt gcttgtccaa
     1201 ggcggtccaa aaggtgttta tgagtttcgc acattaccaa taaatatggg accccaggga
     1261 gaccagtatc ctgaggtaac tcttgcaacc cttatttcgg aaggccccga ggaaaaccca
     1321 atagcacttc ctacaaaatc agaattccct cagatcgagg acttatgtaa taaacctgtt
     1381 aacaagaaaa ggaagttcgt attctcagag aacgccgacg gtaccacatt tttcataaat
     1441 gatgaacagt tcaatcctga tgtcataaac acaacagtcc aattagggga tatcgaagaa
     1501 tggactattc agaacatatc cgaggagttt catgtttttc acattcacca gctcgatttc
     1561 caggtatgtg aggaaaatgg ggtaaaactg gacttcatcg gtcgtcaaga taccgtcaac
     1621 cttccattta tgaccaatgg agttcccggt gaagtgaaga tagcaattcc attcaccgat
     1681 ccggtaatac ttggcaagtt tgtttaccat tgtcacatca tggctcacga ggacaacggt
     1741 atgatggctg tgatcgaagt ttgtgatgat ggtttttgcc ctggtgaatg aggctactag
     1801 ggatatattt ttatacgact gtgcccaatt atatgttata tataaagcct taaacacaat
     1861 tgttttttaa taatcttttt ctcatgtata atcctttttt atcttgaaaa taatagacct
     1921 tagaaaagga gtatttgaaa gaaggcttaa ggggctaagc agagaggtat ctttttctta
     1981 cccacgtatc gagtcagacg ttagaaagat agtcgaagcg gtaagaaaag atggagataa
     2041 ggctttattt aggtatacaa agaaatttga taaagtagag cttactctga aaacagtaaa
     2101 ggtctcgaat aaagagatag aaacagcgga ggctctagtt cctgaagagg taaaatccaa
     2161 tcttgccctt gctgccgaaa ggataaagga ttttcatgaa aggaagctcc cccaggaaca
     2221 cacctttacg gattcccttg ggaatgaact cggctggata attagaccga ttgaaaaggt
     2281 cggagtttat gtcccaggtg gaaaggcagc gtatccatct acagtcctta tgacggtgat
     2341 tccagcaaag gttgcaggag tcaaggaggt atttttagct actccctgcc cgaatggaca
     2401 aatcaaccca gcggttcttg ttgcagcata tatggctgga gtcagcgcag tttataaagt
     2461 aggtggtgca caagcgattg cagctttggc ttttggaact aagtcgatcc caaaagtaga
     2521 taagatcgtt ggcccgggaa acatctatgt gtctattgca aaaaagctcg tatttggaaa
     2581 tgtggatatt gacatgattg caggaccaag tgaggttcta gtaatttctg atggaagcgt
     2641 accagccgaa tgggtagcgt ctgaccttct agcacaagca gagcatgatg agatggcagt
     2701 tgcagtactt gtaacaaact cgcttgaatt tgcaaaacag gtaaagcaag aggttataaa
     2761 aagacttaaa aaacttgaaa gaaagcacat tgctcaagtg tctattcgta atcaaggaag
     2821 aattttcgta gtaaatacac ttgatgatgc catctctgta tcaaatgcca ttgctcctga
     2881 gcatttggag ctctgtgtcg aagacccaaa agctattctt ccaaaggtga agcatgcggc
     2941 ggcgattttt ctcgggtccc tctcaactga ggcgttcggt gactatattg caggcccaag
     3001 ccacgtgcta ccaacaagcg gagcagcaag gttttcctct cctctaagcg tttatgaatt
     3061 tcttagaatg cccagcataa tttcagtttc acaaggtgga tttaaagaac ttggggattc
     3121 tgttattaac cttgcttata gtgaagggtt agaagcccat gcactgtcag ttaaggtacg
     3181 attagatagg tgatttaaat tacttaggct atatctataa acaacctata aaaagtgtaa
     3241 tgtcattgcg aggaacaaag tccgaagcaa tctcaaaaat aatcaataga ctgcttcact
     3301 catttcattc gttcgcagta acaattgcat atagtttgta acttaggtta aatatgaaaa
     3361 gtggagtggt tgtaattgtc caatgtgtct agtagtgaat tccaggatca acttattcag
     3421 gagtaaattt ctttggaaat aagatgttca ttttgtgaaa ttacatctaa agaagccacc
     3481 ggaatcgtac aaagagggga gcactctatt tgttctgagt gtcttaagct tgtaaaggaa
     3541 atcctggatg aatttagcat gggtgatgta tatgaggcga gaggaacgtg ctctttctgt
     3601 ggcagggaac acggtggtgg aaggatggtg tttcagggga aaaatgcgag aatctgtaat
     3661 gagtgtataa acgaaattga ggaggaaatt ggagaaatga aaaatgaaac ttctcctcga
     3721 aagactgaga aaatggggaa tgaaggacaa aagattcaag aagagcctaa gacattatca
     3781 cttttgaaat ttagagtaaa aaaaggcatc ttagagcttt ctccatactc cgttcctcat
     3841 tatgaatgtc ctgtgaaact tgatggaaat gaaagtccct ttcttcttcc gaatgaggta
     3901 ttggaaattg tcattagcga aataagaaag attccaatta acaggtatcc agatcctgag
     3961 gcaagaagcg taagagaaaa aatttcgaag ctgacaggtt tttctttgga tgggattgct
     4021 attggcaacg gttcagatga gcttatagga acgattatgg caaccttttc tggtggaact
     4081 aggagggttc tttatccagt tcctaccttt tcaatgtaca ggattacagg taccgccttc
     4141 gggttagaat tatttgaggt tgagcttgat gacaggtttg atattgatat tagtgaaaca
     4201 ataaaagaaa tcaaaaagaa aaatccggat ttgatctttc ttgcttctcc taacaatccc
     4261 accggaaata ggttttctga tacaagtatt tttgagattc ttgatcaatc aaaaggtatc
     4321 gttgttctgg atgaggcata ctgtgacttt gctgggagga cttttctcaa tttaattcag
     4381 aaatacgaaa atcttatagt tctaagatct atgtcaaagg ttgggtttgc gggcataaga
     4441 atgggaatac tttttggaag aagtgaaatc gtagtagaga taaataaagt acgtcttcct
     4501 tataatgtga attctcttac tcagcgtaca gccgaggtaa tctttgacaa tgtagggttt
     4561 gttagtgaaa acgtccaatt gataacgagg gagcgagata gagtatttaa agtactcaaa
     4621 cttataagtt ctatcgaggc ttttcctagt gatgcgaatt ttatactttt tagggtaaac
     4681 gatgcggata gaatctttaa agagctatta agaaaaggga tattaataag aaactttaat
     4741 tctcccggaa ggcttgagaa ttgcctgcgt gttaccattg gaacaccaga agaaaacaat
     4801 atatttcttg tagcgttggg tgagattttt tcttcataac tatttagcca cggaggtcac
     4861 agagaaagtt tttttaagaa aaaattctgt gaactctgtg ttctctgtgg ctacctattt
     4921 atctttcctc ttaatttcgc caccaataca aaaccaatcc aaaatatcaa gcatccccac
     4981 gggaaaacat ctccaatttt agtgtaaagc gttttctttc cgtctacgat tgctattgtg
     5041 ccttctaaag tagtctgttc aaatatgcct gttcttgctg ttacccttcc gacagaatca
     5101 attattgcac ttatcccagt gtttgtggct cttgcaagaa aacgtcttgt ctcaaccgct
     5161 cttggtatag aaaaagtaag gtgttgatag ggctcgaatg atttaccaaa ccatgcgtca
     5221 tttgtgatat ttacaagtag gtttgccccg ttttcaacaa attttctgct aaaagagggg
     5281 ataatatcct catagcatat aagaggtgca atcttgatac ctttttcttt tatttcaagt
     5341 atttcgagat cgcttcctgg agtgaaatca cctgaggcag gacttatctt ctttaaagcg
     5401 ggaatatagt ttgtaaaggg taaatactca ccaaaaagaa ggagcttaat tttattgtat
     5461 ctcccaagga tctttccctg cgggtcagta agaaatgcag tattaaattt atcaatgttt
     5521 tgatcgaagc taaattcagg gctgaattcg ttgttaaact tgtatgataa ccccccgact
     5581 aaaaagtagg aatttttagt ttgaggaatg acaatctcac cttcgttaat taggaaatcc
     5641 tgggatgctg gaatccatgc ctgaatagct gtctcaggcc agattattaa ctcaggtgaa
     5701 tgtagtacat gtgacattaa cttgtgtctt tcagtgattg tagattcatt tttctgcttc
     5761 ttctcgagaa tatcaaagtt agcttgtacg atacctactt taatttttgg ggcttccgct
     5821 atttttttag tctcaaggtc aatcttccag aggccatagg aaattattag gatgataagg
     5881 atgagagatg ttaagatctc taaagttggg gttatattat gctctaatag tttttttagt
     5941 accttcagta gcgttacgtt tacaaaaatt atcagaaagc ttagtgagta catcccaaat
     6001 aaatcaaaga tttggattaa aggaaggtag tctgcttggg agtttgtaat tccgtatggg
     6061 aagagaaaag ggaaaaggaa ctctattgat gtccagatag aagcaatgag tagcgctgga
     6121 agcaatcctt gttttcgaaa aagttcaagc ttagttgtaa tatatgcaaa gatggcaaaa
     6181 taaaatcctg agtagacgct tattgcaaga tggaagatga agctaacaac atctggaaat
     6241 ccaccaaacc tcgaaagcgt cccgataagc caataggttc caatagcatt agcaatagtt
     6301 cctgttaaga cccccaatcg aaaagcattc cacggggatt tattctctat agcccaaaaa
     6361 aatggtatga taagaaacca tgaaataatc ccggcgtatg gaattacaaa tgcaacggga
     6421 aatagaactc ctgaaattac acatagaatg aggtcgtatt tagtgatctt cattatttta
     6481 taagatacct aagatttcgg ctaccgtctg ttatttaggg tcgtgaaatc tgtagcgggt
     6541 aaagaataat aatgaagaag gataagttgg aatttttgaa aattttgctt acactttctt
     6601 ctcatagagg aagataactg catgagaaca cgtaaggcta aggaaggcga aaagatatgc
     6661 ttacttaagg caactctaaa gaagttttct ggtagagtta aagtctctaa gaccttccct
     6721 catagggttt tagctattcc tcaaggatta acattgtata aattagcaga agccatcgta
     6781 gatagtttcg acttttattt cgaccattgt ttcggatttt atgacaatat aaaggaatgg
     6841 attgattcag ttgagggcta tgaattgttt gctgatatag gagaagagag tgaatttaaa
     6901 ggcgtagaaa ggaccaaagt aaaaaaagtt tttgataaga ttgagaagaa aatgctattt
     6961 ctttttgatt atggcgataa ctggcatttt atcgttgaac ttaaaggaat agaatcgtcc
     7021 aaggagggag aaaagtatcc atttgttacg gagtcatctg gagatgcccc aattcaatat
     7081 ggaccatcag atgactttta gtctatgaat caagatgagt tttatgattc ctataattat
     7141 catgccaatc gacatgagct gggctaggga taaatgagga atagggctcg gagtggtggc
     7201 cctcacaaac tccacaagaa acctttcaag tccggcaagt atcaagaaaa tagaaaataa
     7261 ccatcccgtc ggcgcttgtt tctttctgat tttccagaga tagataaaaa cagcccccat
     7321 taataaaatc tcatagattt gggttggatg tactctctcg gttgttggag gaagtccttt
     7381 tggaaaagcc atcgcccatg ggaggtctga aggaactcca taatcatctc ccacaagaag
     7441 gcagcctacc cgtgctatag cgtatgcaat agcaagacca ggagtcactg catctcctaa
     7501 cgcccagaaa ctcatcttgt ttctataggc gatgtaccac ataagcagtg ctgcaccgag
     7561 aagcccacca aggtatgtaa ggcctcctcg agcaagaaga taatgaatcg gatttcctgc
     7621 gaattcgcta aaagtaacgt tctccaatag gtaaacaatc tttgccccta caattccacc
     7681 taccattgct gcaataagaa aattaccaag aagcctgtca tcaagcccgc gtcttttaaa
     7741 ctcaagcgac gaaagccaat aagcagcaag aaaagcaaat gctaccatta caccgtaaga
     7801 tgagattgta aaatttccga tcctaaaaag ctctggatac attaagctga cctccttttt
     7861 ctgtgagcgg ttccctctgg agccactccc cttgggcttt atcgtgagtg ctcagttaaa
     7921 cgattactat tataggtctc agcatctttt agaagtttaa tctctttttt aagctcggca
     7981 tttttcttta caaagtagca gattaagata aaaagaagaa tccaaaaaat aacctgaaca
     8041 atgaaaatag taaaaagatg gttcatatat tatctcccgg tatttagctg caataaattt
     8101 aacttataac ggcgtctttt caatttatat ttgacgacct aaatctccat aattgtcatt
     8161 gcgagccgaa ggcgaagcaa tcttaagaat gagattcctt cgctcctttc agtcgctcgg
     8221 aatgacagct tttatcatgt catttgcgaa cgcatttaat tttcggtttc actcaagaca
     8281 tgctttattc agagtaagct atgcctacca atctctttga gattcctcac ggagcctata
     8341 ctgagccaat gcgaagtatt cggaatgaca ctacgtgtca gattgcttcg tcgcttcgct
     8401 cctcgcaaag acagtcgttg ggttctttgt cctccgaatg acgaaataag tagtattgca
     8461 tccgcagtgt ggatgcaatg ggaatagaaa gttaatcgtt ttacgatata agaagcatga
     8521 atcagtctca acttgcagtg ctcattaaag gtaggtagat tattatttta atatgttgac
     8581 ttcccctgtc ttggttctta atcgttattt tttcccaatt acagtaacaa gtgtaaagag
     8641 ggcttttgtc atgctctact gtggagctgc aaaggcagtc gctggaaact atcaaacctt
     8701 tgatttcgag tcatgggctg agatttcagc tttgagggaa ggggatgcta tcaaaacagt
     8761 aagtagagta attagggttc cacgagtaat tttacttcta agatatgacc atatgccaag
     8821 aaaggaagca aaatttaatc gtattaacat attcagaagg gatggggatt cctgccagta
     8881 ttgcggaaga aaattctcgc gctcggagct taccctggat catataatcc caaggtcgct
     8941 tgggggaagg agtgtgtggg aaaacgttat ttgttgctgt atgaactgca accgaaaaaa
     9001 gggtggaagg acgcctgatg ctgcaaaaat gaagcttata aataaaccca aaaaaccagc
     9061 ttggagtccg ttaaccaatt tttctataag agctgtgaaa tataaggaat gggagccatt
     9121 ccttaacttc gttgatgtat catattggaa cgtcgaactt gaagagtaga ataattttta
     9181 acttgcaaag gataaaatca acctttagat ctcaaattaa agtgtaagga gagattgtag
     9241 acttgctaaa agtcgggttt gaagatagtt ggtattttta actttagcaa gcgtgtttaa
     9301 aacctagtag ttatcctaca tccttgtatg atagaattat ctcagacttt atactatatc
     9361 ataaactata gtgaacaggt gtaaattata tttcataaga aagccctaga aattaacttt
     9421 ttagtcttta taataatacg tcaaaaaaca aagtaggaag attatgagat acaagatatt
     9481 aattatattt ttaactatag ctattgtttt ttcaaccgac atctttgcaa gaataattat
     9541 tcctcctatt catggaccaa tcataaaaaa gatttctgta tttgttccgg aattatcaaa
     9601 cgtgtctgca ggtggcactg acggaaaagc tcgggaattc attaacgttt taagaaacga
     9661 tctactcaat gctgggttct ttgaagttta cgatgcaagt ggagttagca cagacggaag
     9721 cggcaatata aactttcaag cgtttttcga tgctggcgca gaagcacttg ttaaaggtga
     9781 gtatcaatca tcgggagaca ggatcaatat agccataaga ctctttgacg tcgttcagga
     9841 aagagagctc ctagggcgtt cttatgaggc taattcggga agggtaaggg aagcggctca
     9901 cagattcgca aacgcagtta tgaaagagct tactggtata gatggttttt ttacatcaaa
     9961 gattgttttt gtttcaggca caggacagaa aagggatctt tatataatgg attacgatgg
    10021 gcataataca aaaaggctta ccagtcatag ttcgattatc ctctcgcccg attgctcttc
    10081 agatggaaca aaggttgtct ttaattctga taaggtatgg agtcaggata tttatgttat
    10141 tacactttct cctagagtag aggagaagag gattacccgt ggacttaagc tagagcaaag
    10201 cccggcatgg tctcctgatg gaagaaaaat agcgtacagc gggaatggtg aaatcttttt
    10261 agcaaatgct gatggaacag gcgcagttaa tttaacgcgt cataatgcga ttgatgtttc
    10321 tccttcatgg tctcctgacg gtaggaagat agcatttgta tctgatagaa atggttctcc
    10381 tcagatatat gtgatgaatt cgaacggtag taacattaga aaaattacct ccggaggcta
    10441 taatacagac cctgcctggt ctcctaattt aagtgttaat cggatatcgt ttgtaagggt
    10501 ggagggtaac gaggcaaata tctttacggt aaaccctgat ggctcagatt cccaaaagct
    10561 tacctcaggc tcaagaagaa atgaaaaccc ggcatggtct cctgacggtc actatattac
    10621 tttcggttcg agtagagatg gagctaagaa catttatatc atgtacttga atggagaaaa
    10681 ccagcgttct ctcactaaag gcggtggcaa atcatttccg acctggtgta ggtaaagaaa
    10741 aataggataa gctactattt agaactttaa aaccaatatc taagttattt tttaaggatt
    10801 atagcattca aaagtgagtg cctattattt ataaaataat ataatgtttt aaagcactta
    10861 ggatataacc tctttttttc ttgataaatt cctaaaaacc tttaaaattc atttaagcaa
    10921 actcggcttg tcttttctaa gaaacgaact ctttaagtca tccgaactgt atcataggag
    10981 tttcatatga aggtttttct ttctgaggcg gcttttatgg ggctagtttt aagttcgatt
    11041 gaggtttata aaaaggagtg ccttggactt cttcttggat acaaacttga ggatagatat
    11101 attgttgaat atagcgttgc gtatcagagc gcaaaaagga tgagaaacgg agtttctcca
    11161 aaccgtctta ggcacagtcg aatcgaaggc gttcttccca agtttgataa actcgagctc
    11221 ctgggtgatt ttcactcaca tcctcagtat ggttcactta aaggtattcc agaaccgagt
    11281 tcccttgata taaacgacat gaaacaggga aagctttata tgattgtagc gatcaacgac
    11341 aatgaacatt cagtaagatg gagagaaaat caggatggca ccatatcagg gacgcttaat
    11401 gacttccact ttaagatatc gggatataat tttgaggaaa ataagacgag aaggatcccg
    11461 ataaggtgtc ctttttgttt ggttattgaa aattaagtat ttaatgtttt tgagttaggg
    11521 tcaaaatctt tgagggtttt tattgctgca atcatcccgg acgaaataca aatagaaatt
    11581 gataggtatt taaaggaaat aaaacctaac tgggaagggg taaagtggga gaaatataat
    11641 aagcttcatg taacgcttaa gtttttagga gaggtcgagg agtcatcagt agaaaaaatt
    11701 aaaagcgttc tcggagagtt agttcttcca tgctcatcat ttgaaatgac aattgtgagg
    11761 tttggaggat ttccctatct aaagaatccc cgtgtccttt ttatcggttt gtctgaaaac
    11821 cctgagcttt tgaagctcca tggcgaaatc gaagaaaggc ttgaggttct tggcttcaag
    11881 aaagaaaacc gtaggtttgt tccgcatgta acggtgggta gaattaaagg aagatcaaaa
    11941 tcaagaggca cttttccggc accaaataat acacatttct tcatatctca aatttcagtt
    12001 atgaagagcg agcttaaacc tgaaggttca aaatatacac cgctatctgt gtttcgtctc
    12061 aaggatcgga gtacgtgaat cgtgattcgt ggttggtgat tttttgattc gatgcaagat
    12121 tcccaaatca cgaatcccaa gctacgaacc acgaatttga caaagaataa gaaatcaggc
    12181 aaaaatttag tcgttataga ctaggaggaa tgacaatgca ggcagttata aaaaccggtg
    12241 gaaagcagta tatagtaaag ccgggtgata ttataaaagt tgaaaaaatc gaaggaaaac
    12301 ctggggattc agtggaattc aatgaggtct tgatggttac agactctcag aagggaaaca
    12361 aattaggtct tcctttactt aaggatttta gtatcaaagg tgagattctt gatcatgtaa
    12421 agggcgaaaa gataacagta tttaaattta aaagaaaaaa gggatacaga aggaagaagg
    12481 ggcatcgtca ggtttatact tctatcagaa taacagaggt taatggttag gaggtgaggt
    12541 agatggctca taaaaaaggt ggagggagtt caagaaacgg tagggacagt gaaggcagaa
    12601 ggcttggaat aaaaagattt ggtggagagt ttgttaaagc tggcaatata ctagcgagac
    12661 agaggggaac caagtttcat cctggtgaaa atgtaggact cggtagggat tttacccttt
    12721 tttcgctcgt tgatgggcgt gtaaaatttg aaagaatggg taaggataaa accaaggtaa
    12781 gtgtggaacc acattccaac tgagttatgt atagttcgtg aggttaaggc agagggttaa
    12841 gttcctggtc ttacttcctt aaatcacaac acattttatt caagttaagt acattctgca
    12901 tacagggtct tgaatcataa ccaaataagt gataaagata ttctctgaaa agacccgatg
    12961 gttatctcaa accactttaa ttagcaatta tcatctttag gggtcggtgt gaagatgcgt
    13021 aggcataata tagttttttt tggctccagg gctaaatctt gcatagtcat tgctttgatc
    13081 ttcatagtct tatctatcac cgtctcagtg tttaattccc tttttaatta ctttcagttc
    13141 tttgggtatc aagctaagga agttgaaaaa ctattcgacg gcagaaaaga ttttagtatg
    13201 cttgccaagg atttgcttaa tacaagatcg gacattgctt atgtaaagct cttagataaa
    13261 aacggaatta tgatagatag tctaggaaga gagagtttca ttgatgtaaa gaagcacagt
    13321 tttcttacac ctgataaaaa tacagttata gtcggaataa aagagatgaa gaataaaaaa
    13381 tttgtcctac actctcttct atggagtata gcaataggga ttttcttttc tgctattact
    13441 atctttgcaa ttcttattct ttataataaa aatcagaatc atcacgttga aagggttatc
    13501 accgctatta agcgcatttc acgtggagat ttatctgcaa gactcgatat cgaaaagggt
    13561 cttggaaacg accttttgat gattaggctt tttgagagtt tcaatcagat ggttgatcag
    13621 ttaagaaaga aggaggaagc aaccaaagaa acccacagat ttcaacccac tgttattatt
    13681 tcggaaaaga aggaagaagc taaaattaga agagttattg cctttgttgc aaaggtatca
    13741 gattttgaga acctttcctc aaggctaagt acatcagaat tttcatcctt cctcacagaa
    13801 tatagaaaag cagcttcgtc tatcgtctca gactatggtg gtgttataga agcgctcctt
    13861 caggaagaaa ttgttgcgct ttttaacgtt ccggatgaga aggataaacc agaattgagg
    13921 gctgtctgtg ccgccgtaga ggttctacag gttcttgcaa atatgagcag gaaaaggatg
    13981 atagagggta aagaggcaat aaatgggaaa atagggattg gagcaaaggc cgtgccgttt
    14041 tatgtagagt ccggtatacc acagggtgta aaagaggttg tagcacttgc gaaggggatc
    14101 tgccaagacg caccgctttg gaaggttctt gtttcatctg aggtatataa attcgtaagc
    14161 gactacgttg atgccaaaga gatagatgta gaacacggaa catcattctc gattgtagct
    14221 gttgaagagg gtgtaattca agtatagata gtaattacat gtcttagatc acattgatac
    14281 tcataatttg ttgtaagatc atctcctagt aaacgtcttg aatcacaaac attcgacatt
    14341 ttgagccttc ctcattcaat gttaaaattt tattaacaag aagatttagt tctatgcggg
    14401 atattttctt ttgaagcgta accacaaagg ggtttaagct tattttgata tatccatata
    14461 ggcaggaata gattaatgaa taaaattaat ataaaggatc tcggtccaga agagctagag
    14521 tatttcgttt taaaccttgg tgaaaaatct tatcgtgcga cccagttagg taaatggatt
    14581 tataaaaggg gtgtaaactc atttgacaag atgactgacc tttctaaaga ttttagagcc
    14641 agtctaagtc aggcggctac gatctcatct ttgaaaatga ttgaggaacg catttccttt
    14701 gatggaacga aaaagtttat ttttgggctc tttgatggaa accgaattga aagtgtttta
    14761 attccggaaa atggaagaaa tacactttgt atctccactc aggttggatg tgctttaggg
    14821 tgcacatttt gcctcacagg aagggttgga cgaataagag accttaagac atccgagata
    14881 ttggatcagt ttcttgaagt taataatcgt aataaccgat ctattactaa tattgtcttt
    14941 atgggcatgg gtgaacccct tgataacctt gagaatacag taaaggcctt aaaatccttc
    15001 acacatccag attttattgg attttctcca aaaaggatta ctgtgtcaac ttcgggaata
    15061 gttccaaaaa ttagagagct gggcaagcaa gtctctgtta atctttctgt ctcccttaat
    15121 gcaccgaccg acgagttaag aaacgaaatc atgcctatta ataaaaagta cccgataatg
    15181 gagcttatcg aagagagtgt aaggtttcct gtgccactta gaaaaaccct tacgtttgag
    15241 tatgtgctga ttaatgaagt aaatgattct gataaggatg cgctggagct tgtagagctt
    15301 ctaaaggggg ttagatgcaa ggtaaacctt atccaattta acgaagctta tccacttcca
    15361 tataaatcac cgcctgatga aagggtgctt ggattccaga aggtactttt ttcctacggc
    15421 cttcatgtaa ggattaggaa gaatcgcgga cgtgatatac taggggcttg tgggcagctt
    15481 gcagcagatt atccgctaag aaatagaaaa gccaataatt ttcaacctag gcaaaataat
    15541 tttaaatctg tgtaagtccc tttacatcct ttagatgtgg agtgatatcc ggggaataaa
    15601 cagtaaatat ctcttaagaa ccgttgcaat aaaggggcat gggaatagat atgagggctt
    15661 taatgcttag tattcatttt attggtataa tttaagaaat tatagtaata ggacagggcc
    15721 atttcaatgt ggctaaattg tgatagtgtg caaaaatgaa agcctctgat ataagaaaga
    15781 cttttcttaa atactttgag gagaatggtc ataccattgt cagtagctca tctcttatac
    15841 ctaagaagga ccccacactc ctttttacaa acgcaggaat ggtgcagttt aaagacgttt
    15901 ttctaggagt agataagcgt ccatacacaa gggctacttc gtgtcaaaag tgcgtccgtg
    15961 cgggtggtaa acacaatgac ctggaaatcg tcggtcatac aggcaggcat cacaccttct
    16021 ttgagatgct tgggaacttt tcatttgggg attatttcaa agatgaagca atcaactttg
    16081 gttgggaact tcttactgag atttttaaac ttcctaaaga tagactctgg gtcacgattt
    16141 ttagggatga cgacgaggca ttccatatat ggaaggctga aatcgggctt ccatctgaac
    16201 gtattgtacg tatgggagag atggacaatt tctgggcgat gggcgatagg ggaccatgtg
    16261 gtccctgttc tgaaataata atcgaccaag gcgaagcctt tagctgtgga gagccaagct
    16321 gtgctgtagg ttgtgactgc gaccggtatc ttgagctctg gaatctcgtg tttatgcagt
    16381 atgaaaggga ttccttagga aatctcacac cacttccaaa accgagtatt gacaccggta
    16441 tggggcttga gagaattaca gcggtgcttc aaggggtaaa gagcaattat gagactgatc
    16501 tccttcgcgg attaatctca agcgttgagg agagaacagg aaaactatat ggggaaagcc
    16561 tgtccttgga tgtatcgatg agggtaattg ctgaccactc acgtgctgtc accttcttga
    16621 tctcggacgg tgtgttccct tcaaatgagg gtaggggata tgtcctgaga agaatattga
    16681 ggcgtgcagt tcgtcactca agggcgttag ggatcaagga accatttctt tttagtctta
    16741 cagatcaagt atggtccata atgggcgaag catatccaga gctagaagag agacttagtt
    16801 ttgttagaga ggtagtgaaa aatgaagaag agaggttctt tgaaaccatt gacaggggtc
    16861 ttgagctttt aaatcttgag gtagaaaaac ataaaggtga aaaaatacta ccaggagatg
    16921 ttgcatttaa gctctatgat acctatggct ttcctataga tttaactgag gatatagcta
    16981 gagaaagcgg gttaatagtt gattatcctg aatttgaaaa ggaaatggag aagcagagag
    17041 agagatcaag gcaagcgtgg aagggttcgg gtgaggaaga attagtgcct ctgtacaaag
    17101 agtttacttc aggcgggttt atcgttaact tcacaggata tgagaagact aaagatacag
    17161 ggagaatcac tgcgataata aaggatggaa ggctagttga tagtgcaagt gagggtgtaa
    17221 gggttgaact aatcaccgat aaaaccccct tttacggtga atcaggggga caggtaggtg
    17281 ataggggagt aattgagggt gagtcggggt atgcagaagt actagatact aaaaagcctc
    17341 ttttaacctt gattgttcat caagtatcta taagaaaagg aacactctgg gtcggcgata
    17401 atgttgaatt acgagcagat gagcgtcata gatacggtgc aaaaacccat cacacaacaa
    17461 cacatatgct ccattcggtt cttagggaga cgctaggtac tcatgtgagg caggctggtt
    17521 ctcttgtggc acctgagaga ctaaggtttg atttcaccca cttctcatca attgatgacg
    17581 gtactatccg caggattgag gacatcataa acgatagggt taggtgggat gatagggtat
    17641 taacgcaggt tgacataccg tatgaggagg cgataaaaac cggtgctaca gctatatttg
    17701 aggaaaaata cggagacagg gtgaggattg ttagtattgg tgattacagt aaggagttat
    17761 gtgggggtac tcatcttcac acgactggag aagtcggact ttttaaattg ataggcgaaa
    17821 ctgcttcatc tgcaggagtt agaagaatcg aggccctagc tggggaatct gcttggaggt
    17881 tcattagaaa acaggaagag acccttattg aggcttctag tattttgagg gtaagtcaat
    17941 ctgatttaat atcaaggctt aaaagaatgg tcgaagaaaa cgatagtctc aagaaagagt
    18001 tagagtcatt taaagacaag gtgatgggtg agaagtcaaa gtcaatactt gataatgtaa
    18061 aggaaataaa aggcataaag gttctttctg ctcaggtttc agatgtcggt cctgaagaac
    18121 tcaggaaaat atgggatgag ataagggaaa aacttaagcc agggcttgct gttcttggaa
    18181 gcggaagcga tggcaaggct tatattttgg ttggtgttac aaaggattta agcaaaaggt
    18241 tccatgccgg aaatatagta aaagagcttg ccataataat tgatggcgga ggtggtggga
    18301 agccagacat ggctcaggca ggtggaaata ggcctgagaa acttgaagaa gcgttgagaa
    18361 agatatttga gattgtgaat aggaccttac agtgaattag tcgctttgtc cttgtgtcac
    18421 cccccttttt tctaaaacca tttttatctt tttcaaagcc gtattttcaa tctgtcttac
    18481 ccgctctctc gagagattta gctttttccc cagttcttct agggttagag gactatcctt
    18541 gaggatcctg ttctctatca caaacctctc tctttctttt aaggatttta gtgcggtttc
    18601 taattccctt ttaacctcgt cttcctcttg cgccctggtg actagctctt cttggtttga
    18661 tgcgtcgtct acaagaaaat ctatgtgggt tatctctcca tctcctttta tctcggtatc
    18721 aagtgatagg tctttgcttc ccatcctctg atccatttca attactgctt gatctgatac
    18781 gccaagctcc tttgccaaat ttttataatc ctcaggactc agggtttcat tagcaatgtc
    18841 catcttttgc ttctcggaac gtagtttata gaagagcttt ctttgtgcct gagtggttcc
    18901 aacttttacg aggctccagc ttttaatgat gtaattctga acgtaggccc taatccacca
    18961 tacagcatag gaaattaaac gataaccctt tgtcgggtcg aacctcttta cagcttgcat
    19021 tagcccgata tttccttcct gaattaaatc cattgggttc aaaccgtagt tcctatattc
    19081 atttgcaatc tttacaacga accttaagtt agaagtaata agctttcttg ccgaatctaa
    19141 atcccttttt tttctatatc tcattgcaag cttatattct tcttccctac taagaactga
    19201 gtagttgctt atctcagcca gatagcgctc catagaggta gtaggagcgg gaagtgattt
    19261 attcaagtat tcttcttttt tttctacgtc ttttttaact tcagccatga ttgagtccga
    19321 atcctcacta aatatcctga tagagtcttt ataattatac tttcagtatt gggtctattc
    19381 aacgttaata gttcttcaga taaccttctt ttcaatgaga aaaaggagta agctagaggt
    19441 tgaaattcgt aacattctaa gtgaggaatt gttggcctca ctaaggaata gagaggcatc
    19501 ttcacttctt tatttatttt ttcgattttg cgaaattatc aagaaacatg gtaaaaatag
    19561 aataaggctt aatgacttat tgcggcctcg ggcaaatata tttaggttac aatctaattg
    19621 ggaaaagctt tctaagggac caaattttcg aaccatcttt gaaatggatt caggtaatta
    19681 tattcaaatt aatagcagat ttgagagaca tcttggcgtt atctgcaata aatctagact
    19741 ttactgggat tttatgtcta gcttattttt caaaaaagaa gcgttcaaag gtggaatcga
    19801 aggagagata aaaaaaggcg ttctcctttt taacgaaggt ttttacttcg agtgccatga
    19861 gtttcttgaa gaggtctgga gaaaagagaa gggaagagaa aaagaatttc ttaagggatt
    19921 gattcacgtc gcagttgcat tttatcatct agaatacgaa aattataaag ggacaattaa
    19981 ttatctgagg agaagttata agagactaaa ggaatttgaa cctctattta tgggtgtgga
    20041 tattaagaac ttcttagctg agttagagag ttatttaaaa aaccttgaaa aatcgggcaa
    20101 acataatctt aaatttttga agaatgcaat tccaaggatt cgactgattg agtgaaatat
    20161 caggttttag ttatgaagat tcgattaaac aattgtgcat tccaaaatct acggtgcgtt
    20221 ccccgaagga gctcccgatt taatcggagg gctgatgagg gcatcagccc ctacaatttc
    20281 cgttgtgcaa gtatgaagta attaagaata aatgcacaag atttttttgg actggtaatg
    20341 ctagttattt gttgtttgtc tatgactcta tactaacgac taaaaactaa agactcgtac
    20401 agactcatac agttacttga ctgaatcgaa ggttcaattt acatttatcc tggctccggt
    20461 tcggagttta atacaaaatc agatccggcg tagctcagtc ggcagagcga gtggctgtta
    20521 accactgggt cgggggttcg aatccctccg ccggagccaa atttcctcaa atattccaaa
    20581 tagttacata gcataatgaa agcttaagtt cggataatta ggctccaaaa ttgcaaccct
    20641 ttatagaggg aattgtaggg ctcgttccct gaacgagctt ttagggctga tgaaggcatc
    20701 agtcccttta ggaggatgga atgcagacta atacttttgg tttttaggtg ccctgtggct
    20761 ctgccacggg aggttcactc taagcaacta taaggtcaac tttggatata tacctttatc
    20821 atatgctcct gttgcaggta aggcccctaa aacttctata ttatctgcat tgaaaagact
    20881 ggaacctgca ccaaaaacgg ctcccccctc taagtatgtt ttcttgctgt gcggagctag
    20941 gactacattt aagacccctg tttcattacc tatttcagta ccataattag aagtcgaagt
    21001 agagtaataa tgtactctta cctcaacgtt tttataggct atatcactag tattttcaat
    21061 cgtgacatga tggataatac ccactcccct tgtagcataa aaagaccagt ccgtgatctt
    21121 tatataccat tccgggtgtt gagccatagt aatagtattt ggggtttctg tttcttcaga
    21181 tagtccagcc agattgaata aagtaaatag aattaagccg ctaatagcta tagttttcat
    21241 tttacaattc tcctttctta ggaatacttt acatgctcaa attgattctc cacagcaact
    21301 cataatgcct tatataattt tatcacagtt tctctaacta catatagcca tttttgttat
    21361 agaacttgtt gattatatat gtagagagct cgctttttta cctaatttct tttccagttt
    21421 gtctaactga gtaagatttg tcttcagtcc gttactgcgt attcaactga aagatggt
//
LOCUS       DATDRL010000140         7139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2969700, whole genome shotgun sequence.
ACCESSION   DATDRL010000140 DATDRL010000000
VERSION     DATDRL010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2969700"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VI935_05080"
     CDS             <1..579
                     /locus_tag="VI935_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25006.1"
                     /translation="VASYLLLKSGGKVLSPAKQVNHIASAGLKEFGMIRVKNAPKSQI
                     PRCLREITRASLTQFDWRKALKLEHNKIERLFLVNLSYLTTDATGWVMSMDVIMDNIL
                     NKLAQHDSSIGQYICGQIGAFIGSSRSRFSRKYPAFYKACKEIHKYRKQADLAHPIDR
                     ATGKRTERIKFSVIKNVRPILYHGFQELIREW"
     gene            complement(903..1271)
                     /locus_tag="VI935_05085"
     CDS             complement(903..1271)
                     /locus_tag="VI935_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25007.1"
                     /translation="MKTLEDIRLDKDLQFQIHWDMKPRERIRRTRIETEEEMAQIEKQ
                     LQARVGYYFYIDVRNRHAGLYLYENYPDGSGRFVAEITEISEEMLQEAVREAGGKIET
                     DGHYPINASIKTWLKNQLAD"
     gene            1552..2169
                     /locus_tag="VI935_05090"
     CDS             1552..2169
                     /locus_tag="VI935_05090"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HLE25008.1"
                     /translation="MNSPNVSKIFFLSVLIFAFVFITFAAVVKAEDKASDQNQDAVMA
                     KWKEYATPNENHKILDSFVGKWNYTVKWWMSPGAKPEVSNGTSNVKWIIGGRFLKQKA
                     EGTSMGQPFKGMGITGYDNAEKQYNSIWIDNMGTGMMMSKGNYDPSTKALVEKGTFTD
                     PLEGEKSYRGVTQIINQDKYTYEIYISGPDKKEFRMMEIVYVRSK"
     gene            2171..2455
                     /locus_tag="VI935_05095"
     CDS             2171..2455
                     /locus_tag="VI935_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25009.1"
                     /translation="MRPFLIITIAIILSSGCTTSPQIQTIQEQSVGKIPCPSEKIEII
                     QYKINEADGSEYWMALCNGRTYKCKRGASDQTKTISPDVICEEMETQMPE"
     gene            complement(2667..3044)
                     /locus_tag="VI935_05100"
     CDS             complement(2667..3044)
                     /locus_tag="VI935_05100"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HLE25010.1"
                     /translation="MDGSYQNTAQAVDAVNSTHTDSENATYVVFMDTTPPTVSITNPI
                     NGTTVSGTVNIQVSASDSLSGVNRVEIYLDGSLKKTDTAAPYDYSWNSRKAANGTHTI
                     TAKGYDQQGNFSQPSITVTKVKR"
     gene            complement(3739..4758)
                     /locus_tag="VI935_05105"
     CDS             complement(3739..4758)
                     /locus_tag="VI935_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25011.1"
                     /translation="MMYTKSLWIGLVFSVLVYLYGEPLWAEPILCNNELDCPGFCVKG
                     TCQERVPCDSDSDCTPLNQTRGSNTGVEFGCVEGGICDRVECNTAADCNTGNACIEHR
                     CEGCDNNAQCGERGVCQNNECMCVECTQNRQCAFDKRCENNSCVDFCDEDREFVAYQD
                     GDTPCRACVDTRFAQRCPDPRCLPPGKICASGFCVSPCAPSDLEVFERGGLLEELIED
                     LERLRTQPDPSGLPDCPRCTIGFDMIGVRGVLERAGMTKPVHISLLDSSGKVVADFGT
                     FSPKEQSWATIPELMQPNLIQSVAIQGGCGYMLEISDPKSKEAARGAICLEPRKKPLP
                     LKRAL"
     gene            complement(4835..5941)
                     /gene="mqnE"
                     /locus_tag="VI935_05110"
     CDS             complement(4835..5941)
                     /gene="mqnE"
                     /locus_tag="VI935_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HLE25012.1"
                     /translation="MARLLENIERLCSDKSLLEILEKVDAGERISFEDGLRVLKSDDL
                     NAIGMIADYVKRKREGDKVYFVVNRHVNPTNICAISCKFCAFGKPQGAAGTYEMSFDE
                     ILAGLNEELREVHVVGGLHPDWPFERYLDIVRVIKNAYPDTHVKAYTAVEIDWFSQIS
                     GLTVEEVLIMMKGAGVDALPGGGAEIFAEDVRKKICSPKTSSQKWLEIHKIAHQVGIP
                     SNATILYGHLEKYEDVVDHMDRLRNLQDENNGFFAFIPVLFQPENTRLKHIKPFPGSY
                     DLKVHALARLYLDNFPHIKAYWITLGEKTAQLALHFGCDDADGTIIREKIIHDAGAPS
                     EVGHTRDFFVKIIKDAGYTPVERDALYNVVRVYN"
     gene            complement(5917..>7139)
                     /locus_tag="VI935_05115"
     CDS             complement(5917..>7139)
                     /locus_tag="VI935_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HLE25013.1"
                     /translation="WHPHKGGAAHYHEAAKLGMDLEAAVILGGDPKMIFTAIAPLPEG
                     MDELAFASYLRGSPIPMVRGKSISLRVPANAEFVIEGIVPQGETRVEGPFGDHFGHYS
                     LEADFPVFKVKEITRRHNAIYPATVVGKPPKEDIFLGIAAAEMFSPLVRIIQPEVKDM
                     WAYPESGFHNLLVVSVDERYAKNGIKAMLAPWGIGQLLLTKCMIMVSSDVNPKDFSAV
                     LREIGENFDPREDFLLIPWAPVDTLDFTSRKLNVGSKMGINAVRRANGSDRKRKIPER
                     VPDPRERLSEVSDFRLLPGGFLVLKITRNPKETIKRLFDMPGYEDVRIVVALSPDIDL
                     KDNTELIWGIFTRFDPYLDVIFERTELRGSAVVYGGRMGIDATFKEWYPEVIEMSEDI
                     KKMVTERWQDYWRT"
BASE COUNT         2059 a         1610 c         1361 g         2109 t
ORIGIN      
        1 gtagccagct atttattact caagagcgga ggcaaggttc tctctccagc taaacaagta
       61 aaccacatag caagcgccgg attaaaagaa ttcggcatga tccgagttaa aaatgcacct
      121 aaatcacaaa ttcctcgttg cctaagggaa ataacaagag caagtctaac gcagtttgat
      181 tggcgtaagg cattaaaact agaacataat aaaatcgagc gtttgttcct tgtaaatctg
      241 agctatctga cgacggatgc aactggctgg gtaatgtcaa tggatgttat catggataat
      301 atcttaaaca aacttgctca gcacgattct tctatagggc aatatatttg cgggcaaatc
      361 ggggcattca ttggctcatc cagaagtcgc ttttctcgta aatatcctgc gttttacaag
      421 gcatgtaaag aaatacacaa atatcggaaa caggcagatc ttgcacatcc aatcgatcgt
      481 gctactggta agcgaacaga acgcataaag ttttcagtta ttaaaaatgt tcgtccaata
      541 ctttatcacg gatttcagga actgattaga gaatggtaaa attatatttt tacaaaaaag
      601 taattttaat aacaattctt aggacaaaaa gtaagttttg tttttttaag tttatctata
      661 caaatgcatc ttaactactt tataaattca tagccggcgt ctcaaattcc ttcaaattaa
      721 ctctaagctc ctcttaagtt aaataatgat ttgttacttt gcttgcaaaa tatgctccat
      781 aggcgctcat tccaaaataa ccatggtcgc agaggttgaa tgatatggtt ttagattttc
      841 ttcttccttt gctcttttag cttttcaatg accagaggtt gcgtgagtgg cttcgaggct
      901 gattaatctg ccagttggtt tttcagccaa gtttttatcg aggcatttat ggggtagtga
      961 ccgtcggtct ctatctttcc acctgcttcc cgaacggctt cttgtagcat ctcttcagaa
     1021 atttctgtta tttctgctac gaatcttccg gagccatccg ggtaattttc gtaaaggtat
     1081 agtcctgcat ggcgattccg gacatctatg tagaaatagt aaccaaccct tgcttgcagt
     1141 tgtttttcga tctgtgccat ttcttcctcg gtttctattc ttgtccgtcg aatcctctcc
     1201 cgtggcttca tgtcccagtg gatttggaat tgcaggtctt tatccagccg aatatcttct
     1261 agagttttca tttgttattt ctactgttat ttctccttgt tgtttaagaa cctttatagt
     1321 agtgtgtgga ttttacgttg attcttgcgt tttttttgtt acagtgtacc catacgcggg
     1381 tgaatagaca attaatatct atgagattgc aataaccctt ctgccaatat aagagctgaa
     1441 gtagggttag acgaaaatta tgggttttct aaaaaacagc tcaaactatt tcttctaacc
     1501 ctttgaccag ttacaattat taaacattta atgatttagg agggaatatt aatgaattca
     1561 ccaaatgtat caaagatatt ctttttgtca gtgcttattt ttgcttttgt ttttataacc
     1621 ttcgcggcag tagtcaaagc agaagataaa gcctcggacc aaaatcaaga tgccgttatg
     1681 gcaaagtgga aggaatacgc tactcctaac gaaaatcata aaattttaga ttcattcgtt
     1741 ggaaagtgga actatacagt taagtggtgg atgtctcctg gtgcgaaacc agaggtatcc
     1801 aatggaacca gcaacgttaa atggattatt ggcggccgtt tccttaagca gaaagccgaa
     1861 gggacatcga tgggtcagcc gtttaaaggc atgggcataa caggatatga taatgcagaa
     1921 aaacaataca attccatttg gattgataat atgggaacgg ggatgatgat gtccaaaggt
     1981 aattatgacc cgtccacaaa ggcgttagtc gaaaagggaa catttacaga ccctcttgaa
     2041 ggcgaaaagt catatagagg tgtgacgcaa attatcaatc aagataaata tacttatgaa
     2101 atatatattt ctggacccga taaaaaagaa ttccgaatga tggaaatcgt ttatgtgaga
     2161 agtaagtaaa atgagaccgt ttctaataat tacaatagca attatactta gctcaggttg
     2221 tacaacctcc ccacaaattc aaactataca agaacaatct gtcggtaaaa ttccatgccc
     2281 ttctgaaaag atcgaaataa tacaatacaa aattaacgag gcggatggca gcgaatattg
     2341 gatggctttg tgcaatggga gaacatacaa atgcaaacga ggcgctagtg atcaaacaaa
     2401 aactatcagc cctgatgtaa tttgtgaaga gatggaaaca cagatgccgg aatagaagtt
     2461 taatgaaaaa ggtgacgcaa ccttaaaaaa catacagatt tcctgagtct aagaaagaga
     2521 cgcttccatg ggtatgaaga caatgttaag gttttttatg acctgcctaa gaacgtttag
     2581 ttttcagaag tctttgcagt aaaaagctta taattcaaga ccccttctat tctatagctt
     2641 ttgccaccac catcagtact accgctttat ctttttactt ttgtcacagt tattgaaggt
     2701 tgcgaaaaat tgccttgttg gtcataacct tttgcagtaa ttgtgtgtgt cccatttgct
     2761 gccttcctcg aattccatga gtaatcgtat ggagccgcag tatcggtttt cttgagactc
     2821 ccatcgaggt aaatttcaac acgattaaca ccgctaaggc tgtctgaagc cgatacctgg
     2881 atatttacag taccgcttac tgtggttcca tttatgggat ttgttattga aactgtagga
     2941 ggggttgtat ccataaacac tacataagtc gcgttttccg aatcagtatg agtggaatta
     3001 acagcatcta cagcttgtgc agtgttctgg tagctaccat ccacagcact agaacttgaa
     3061 gtaacgctcc atgttgaaga accctgttgt cctgaattta aatttaagac tgaggaagaa
     3121 aaacaggaaa accaaccact tggtactaaa ccgctcaagt taacggaaag gggaaagggg
     3181 tcgagtcttt tgttgatata ttcttttatt tggtcctcga aatctcaaaa ttatttttaa
     3241 ggtattatga tgctaattgg tttttattca tcatatgatt ctcgaatcta cttctaatta
     3301 gaaattagtc aacaaaagat ttgacaccac aaccacaacc cacaaccttc gtcattttcc
     3361 gtgaggcagc gaattaaact ctcgaataga tttttatatg aggcaatagt caatattagc
     3421 ctatccgaga ttccaacact ttacgccaca acaatcacta aaaccatccc actgcaatcc
     3481 ctgagatgta cccctcttta gttaacctgc ttaagtaaag cttaaaaaca acaggtgctc
     3541 tagaaagcaa aaataggatt atgattgctt gaatatgtcg caaagggaaa ctgtctgtcc
     3601 atgtttctta aaaaaccttt tagataaaaa gtgtgattat tcaaaaccta ctccatttgt
     3661 tcagaaatct ttcctaaaac aacaaaataa caaaagcctg acccgttttt ctccaatggc
     3721 agcccggtgg tagcggcctc agagcgcgcg tttcaggggt agaggttttt tcctcggctc
     3781 aagacagata gccccgcgtg cagcctcttt gctctttggg tcgcttattt caagcatgta
     3841 accacaaccc ccttggatcg caacagattg aattaggttc ggttgcatca gctctggtat
     3901 tgtagcccag gactgctcct ttggtgaaaa ggtaccgaag tcagctacca ctttccctga
     3961 actatcaagt aagcttatgt gtacaggctt agtcatacct gctcgctcaa gaacaccccg
     4021 cacaccgatc atatcaaaac caatggtaca tctaggacag tctggaaggc cacttgggtc
     4081 gggctgtgtt ctaagtcgtt ccaaatcttc aatgagctct tcgaggagcc cgccacgttc
     4141 gaaaacttcc aggtcgctag gtgcgcatgg tgacacgcag aagcctgacg cgcatatctt
     4201 ccccggagga agacaccttg ggtctgggca acgctgcgca aaccgagtat caacacaagc
     4261 cctacaggga gtgtcaccgt cctgataagc cacgaactcc ctgtcctcat cgcagaagtc
     4321 cacacatgaa ttgttctcgc acctcttatc aaaagcgcat tgcctgttct gagtacattc
     4381 aacgcacatg cactcattat tttggcagac cccgcgttcg ccacactggg cgttgttgtc
     4441 gcatccttcg cagcgatgct caatgcatgc attgccggtg ttgcaatctg cggccgtgtt
     4501 acattccacc ctatcgcata tgcctccttc tacgcaacca aattctacac ctgtattact
     4561 acctctagtt tgatttaatg gggtacagtc gctgtcacta tcgcacggga ccctctcttg
     4621 acaggtgcct ttaacacaga atccagggca atctaattca ttgttgcaaa gaattggctc
     4681 tgcccataag ggttctccat acaggtaaac aagaacagaa aacactagac caatccacaa
     4741 gctttttgta tacatcatct tcctttcctc ctttacaagt taaatggtca actcttgaat
     4801 cccgcagact tcattctaac acaatcatca attcctaatt atacaccctc acgacgttat
     4861 aaagcgcgtc tctttcaacg ggggtgtaac cagcatcctt tattatcttt acgaagaaat
     4921 ctcttgtatg acctacttct gacggggctc cggcgtcatg gatgattttt tctcggatga
     4981 tagttccatc cgcatcatca catccaaagt gaagggcaag ctgggcggtc ttttcaccca
     5041 atgttatcca gtacgccttt atatgaggga agttatcaag atacaaacgg gcaagggcgt
     5101 gcacttttaa gtcgtaagaa ccggggaagg gttttatgtg tttaagcctc gtattttcag
     5161 gctgaaatag aacaggaatg aacgcaaaaa atccattatt ctcatcttga agatttctaa
     5221 gtctatccat atgatcaaca acatcttcgt acttctccaa gtgaccatag agaatcgtcg
     5281 cattggaggg aatcccaacc tggtgggcga tcttatgaat ctcaagccac ttttgagacg
     5341 aagtctttgg cgagcatatt ttctttctaa cgtcttctgc aaagatttca gcgccgcctc
     5401 caggaagcgc atcaactccg gcccctttca tcattattaa aacttcttcc actgtaaggc
     5461 cactgatctg ggaaaaccag tcaatctcaa ctgctgtgta agcttttaca tgagtgtctg
     5521 gataagcgtt ttttatcaca cggacaatgt ctaaataacg ctcaaatggc cagtcaggat
     5581 ggagtccgcc tacaacatgg acctctctta actcttcgtt taaacctgca agaatttcat
     5641 cgaatgacat ctcataagtt cctgcagcac cttggggctt cccaaatgcg cagaacttgc
     5701 atgagattgc acagatattt gtaggattaa cgtgacggtt tacaacaaaa taaaccttat
     5761 ccccttccct ttttctctta acataatccg caatcatacc gattgcattt aggtcatcac
     5821 tcttaagaac cctaagtcca tcttcaaaac ttatccgttc tcccgcatca accttctcta
     5881 gtatttctaa tagggattta tcgctgcaaa gcctttctat gttctccaat aatcttgcca
     5941 tctctcagtc accatctttt ttatatcctc tgacatctca attacttcag gataccattc
     6001 cttaaaagtt gcgtctatcc ccattcttcc accataaact accgccgagc ctctgagctc
     6061 agttctctca aatataacat caaggtatgg atcgaaccta gtaaatatac cccaaattag
     6121 ttcagtatta tcctttagat caatatcagg actcagggct acaacgattc ttacatcttc
     6181 atatccaggc atatcgaaaa gcctcttaat agtttcctta ggatttcttg taatctttag
     6241 aactaaaaac ccaccgggaa gaagcctaaa gtcgctcacc tcgctaagtc gctctctcgg
     6301 gtcaggaacc ctttcaggaa tctttctctt tctatccgag ccatttgcac gccttactgc
     6361 atttatcccc atcttacttc ctacattgag tttcctactt gtaaagtcca gggtatcaac
     6421 cggcgcccag ggaatcaaga ggaaatcctc tcgaggatca aaattctctc ctatttctct
     6481 aagaactgca ctgaagtctt ttggattcac gtcactcgaa accattatca tacattttgt
     6541 aagtagaagt tgccctattc cccagggagc aagcatagct ttaatcccat tttttgcata
     6601 cctctcgtca actgaaacca caagtaggtt atggaatccg gattccggat atgcccacat
     6661 atctttaact tcaggttgga ttatccttac aagtggtgag aacatctctg ccgcagcaat
     6721 tccaaggaat atatcttctt taggcggctt tcccacgacc gtagccggat aaatcgcatt
     6781 gtgtcttcgt gttatctctt ttaccttaaa aactggaaaa tcagcttcca atgaatagtg
     6841 cccaaagtgg tctccgaagg gaccttccac tcgtgtctct ccctgtggaa caattccctc
     6901 aataacgaat tcggcatttg ccggaactcg aagcgaaata ctttttcccc taaccatagg
     6961 aatgggactc cctcttaagt agcttgcgaa tgcgagttca tccattccct caggaagggg
     7021 agcgatagca gtaaaaatca tcttgggatc accgcccagg atcacagcag cttctaagtc
     7081 cattccgagt ttggctgctt catgataatg agctgcaccg cccttatgcg gatgccagt
//
LOCUS       DATDRL010000141         3592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_134097, whole genome shotgun sequence.
ACCESSION   DATDRL010000141 DATDRL010000000
VERSION     DATDRL010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134097"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..259
                     /locus_tag="VI935_05120"
     CDS             116..259
                     /locus_tag="VI935_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25014.1"
                     /translation="MRPRLKGVPPEFEEDSEKGKARLEKPALLTVNLVQLRHNRQPTS
                     TPP"
     gene            complement(342..941)
                     /locus_tag="VI935_05125"
     CDS             complement(342..941)
                     /locus_tag="VI935_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25015.1"
                     /translation="MSFDCVYIDRFSCRDRPNPAVSQSTCVCPDDGQSHWIAPTFLKQ
                     KKEVITMKKLSMLTIAVLATIMVFWSVLPAKAIIVVGGKFGMVGITMGQTARLNVVNV
                     IDLNQISTGDRNECMVELMFFDSDGEMLAMRMVSLDPGMAAFHDLRFPADVEGRFQIR
                     AEVMIMGDKMSCNVIPTLEIYDNETMKTEVFETITLDPY"
     gene            complement(1319..1741)
                     /locus_tag="VI935_05130"
     CDS             complement(1319..1741)
                     /locus_tag="VI935_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HLE25016.1"
                     /translation="MTNLSVAENKISSIKRYLSILKSYQGYSQKEIGRDVTLRGAIER
                     YLYLVCQAVIDLAEVIISISKFRKPVTLSESFVILAEEKVIADELKDKMVKLVGFRNV
                     LAHEYEELNYEILYDVLKIRLQDIKEFLKVSERFLNLK"
     gene            complement(1749..2129)
                     /locus_tag="VI935_05135"
     CDS             complement(1749..2129)
                     /locus_tag="VI935_05135"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLE25017.1"
                     /translation="MVERLKSVFHSYPQIKLVYLFGSQAKGDTGPLSDYDFAIYFDDK
                     DPKKMFNIKLELLDQLSRILMADKIDIIILNLSKMPEFKFNVIKEGKLIYEEEPFRVM
                     VEPKILNEYFDFKLLLSKYQLTKA"
     gene            2261..3361
                     /locus_tag="VI935_05140"
     CDS             2261..3361
                     /locus_tag="VI935_05140"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HLE25018.1"
                     /translation="MSKITVGVIFGGRSVEHEVSLLSAKSIIRNLNPDEYQIFPIFIE
                     KNGTWRKASIDGWIKDGDLKIFTNSFLSPSLNPEKPVFYELDTNRVEGEHRVDVLFPV
                     LHGTYGEDGTVQGLFELMGIPFVGASVLGSSIGMDKIAMKTIFREAGLPVVKFAGFYS
                     YEWKLQREIITKNVLSEIVVPCFVKSANLGSSVSITKVKSEDKLEKAIDFACTFSHRI
                     MVEKAVLNPREIEVSVLGNENPIASLPGEIIPHREFYDYIAKYIEDGTALIAPANLEK
                     GLVEKFQDYAIRAFKALDCEGMGRVDFLMEKSSSEIFVSEINTIPGFTQISMYPKLWE
                     VSGIKYPELVSRLIDLALDRHEMKKKLRTDIK"
     gene            complement(3383..>3592)
                     /locus_tag="VI935_05145"
     CDS             complement(3383..>3592)
                     /locus_tag="VI935_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLE25019.1"
                     /translation="QFQSIKLLDVSHSTAVMPLESLFVVLFSYFIFQDLPSVIKLIGG
                     AGIVMGVVFLVIFRGEKVDLEVSED"
BASE COUNT         1127 a          728 c          696 g         1041 t
ORIGIN      
        1 caggccaaat gagcacaaat cgcccttcga cgtaggcacg aacttgttcg tgcccaaaaa
       61 ttgcacagac aagtctgtgc ctacgttatt cgaacagtta tgaattggaa atgatatgag
      121 acctcgtctt aagggtgtcc cgcctgagtt cgaagaagat tcagaaaaag ggaaggcgcg
      181 gttggaaaaa cctgccctac taaccgtgaa tttagttcag ttaagacata accgccaacc
      241 cacttcaacg ccaccctaag cgaggctagg tgaccagctc cacttctttt atccactgca
      301 actgaccgag atctgcaatc tcagactcac aaatcaatca ttcaataggg gtcgagtgta
      361 atcgtctcaa aaacttcggt tttcattgtc tcgttgtcgt atatttcaag tgtgggaata
      421 acattacagc tcatcttatc gcccataatc atcacctctg cccggatctg aaaccgccct
      481 tcaacgtcgg cagggaatct cagatcgtga aacgccgcca tgccaggatc aagagacacc
      541 atcctcatag ccaacatttc accatcgctg tcaaagaaca tcaactccac catacactca
      601 ttccggtcac cggtacttat ttggtttaga tcaataacat tgactacatt gaggcgtgct
      661 gtctgcccca ttgtgattcc taccatgccg aacttaccac ctacgacgat aatggctttc
      721 gctggcagta cagaccagaa caccatgatt gttgccaaaa ctgcgattgt cagcattgat
      781 aactttttca ttgttattac ctcctttttt tgttttaaaa atgtaggagc aatccaatgt
      841 gattgcccat catcagggca gacacaggtc gactggctca ctgcaggatt gggtctgtcc
      901 ctacaagaga atcgatcaat ataaacacaa tcaaatgaca tcacctcctt ttaaaacatt
      961 tatatcaaaa cgttgataaa cttccattca ccacctctgt ggtcttttta tagctaacag
     1021 cgaaactatt tggaatcttg actaggtagg agcggcttcc agccgcgaca ctgtcgcgcc
     1081 aaggctatcc tgagcctgac gaaggaatgg cgctcctacg tgaaaagagc gataaaacct
     1141 gaagaaacgg aatgtgaata aatctcttag gggtgtggag ttttggttgg ttggttggtt
     1201 ggttggttgg ttggttggtt ggtagaaatt tcatgttgtg taacgattaa atttcaatct
     1261 aaagaccgaa gtcaacccta aattttgtag gggcggatga aactgccctt acttgatatt
     1321 acttcaaatt tagaaatctt tctgaaacct tcaaaaactc ctttatatct tgaagcctaa
     1381 tcttaagaac atcataaagg atttcgtaat ttaactcttc atattcatgg gctaaaacat
     1441 ttctaaatcc aactagcttt accattttat ctttcaattc atccgcaata accttctctt
     1501 ctgctaaaat tacaaaagac tcgcttaggg tgactggctt tcggaattta gaaatagata
     1561 taattacctc agccaaatca ataactgctt gacatactaa gtataaatat ctttcaatag
     1621 cacctcttaa ggtaacatct cttcctattt ctttttgtga ataaccttga tacgatttga
     1681 ggattgatag atatctcttg atagaactta ttttattttc tgcaacagaa agattcgtca
     1741 tacttttttc acgctttggt taactgatat ttgctgagaa gaagtttaaa gtcaaagtac
     1801 tcgtttagaa tttttggctc taccataact ctgaatggct cctcttcgta gattagcttg
     1861 ccttctttta tgacattgaa tttaaactcc ggcatctttg ataaatttaa aattataatg
     1921 tcaattttgt ccgccattaa aattctactc aattgatcta gtaactccag tttgatatta
     1981 aacattttct ttggatcttt atcatcaaaa taaattgcaa aatcatagtc acttaatggt
     2041 ccggtatctc cttttgcttg tgagccaaag agataaacaa gttttatttg agggtagcta
     2101 tgaaatacgc tctttagtct ttctaccaat acctcattca tgctttaaat gatatgagat
     2161 tggaacctaa aaatcaaaag atagagcacc tcagccttcc ttgacaatca agctgtgctt
     2221 gggtaaccca ttagcaaata attcattgga gaaaaaataa ttgtcgaaaa ttacagtcgg
     2281 agttattttt ggtggaaggt cggttgagca tgaagtatct ctgctctcgg caaagtcaat
     2341 tataagaaac ttaaacccag acgaatacca aatctttccc attttcattg aaaaaaacgg
     2401 aacgtggcgg aaagcgtcta ttgatgggtg gataaaggat ggggatttga aaatattcac
     2461 taactccttt ctttctccct ctttaaatcc cgagaaaccc gtattttacg aactcgacac
     2521 aaacagagtc gaaggagagc acagggttga cgttctcttt cccgttcttc atgggacata
     2581 tggagaagac ggaactgtgc agggactctt tgaacttatg ggaattccat ttgtcggcgc
     2641 ttcagttctt ggctcctcaa ttggaatgga taaaatcgct atgaagacca tctttaggga
     2701 agcgggactt ccggtagtga agttcgctgg attctacagt tatgaatgga aattacaaag
     2761 agaaataata acgaagaatg ttttaagtga aattgtagta ccctgttttg tgaaatctgc
     2821 aaatcttggc tcaagcgtca gtataacaaa ggttaaatca gaagataagc tagaaaaagc
     2881 tatagatttt gcctgtacct tttcacatag aatcatggtc gaaaaggctg tcttaaaccc
     2941 aagggagatc gaggtaagcg tccttggaaa tgaaaacccg attgcatccc ttcctggaga
     3001 aattatccca cacagagaat tctatgatta catcgcaaag tatatcgaag atggcacagc
     3061 gctcatcgct ccggcaaatc ttgaaaaagg attggtagaa aagtttcaag actatgccat
     3121 tagagctttt aaagcgttag actgcgaagg gatgggaagg gtggatttct tgatggaaaa
     3181 atcatcgagt gagatatttg tgagtgagat aaacacaatc cccggtttta cgcagataag
     3241 tatgtatcca aaactctggg aagtaagcgg aataaaatat ccagaacttg tatcgcgact
     3301 aatagactta gcacttgata gacatgagat gaaaaagaaa ttgagaaccg atataaagta
     3361 aagagttaga acgaaagttt atttaatctt cacttacttc cagatcaacc ttttcaccac
     3421 gaaatataac taggaatact acacccatta caatccctgc tccacctata agctttataa
     3481 cggagggtag gtcctgaaaa atgaaataag aaaaaagaac aacaaaaagg gattcaagag
     3541 gcatcacagc agttgagtgg gaaacgtcga gaagcttgat tgactggaac tg
//
LOCUS       DATDRL010000142         3609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1596977, whole genome shotgun sequence.
ACCESSION   DATDRL010000142 DATDRL010000000
VERSION     DATDRL010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596977"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..572
                     /locus_tag="VI935_05150"
                     /pseudo
     CDS             244..572
                     /locus_tag="VI935_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015551288.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
     gene            752..1279
                     /locus_tag="VI935_05155"
     CDS             752..1279
                     /locus_tag="VI935_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25020.1"
                     /translation="METLFSTIQHYLPFLFTGISLISIGISVKSYRMASRYQNFDYAV
                     KLQTIDEIIKFGSPSSPEFNYSTKIENRGQKPVKIERVYIDYGSRDDPKKRIKHVIEG
                     QFYLRPGEPREIKLNLSQSEVESIRQKFDRQCMFFLRVLYHTAEGRIAEVTRFLGGYD
                     KDMIIFVVQRGESLT"
     gene            complement(1331..2614)
                     /locus_tag="VI935_05160"
     CDS             complement(1331..2614)
                     /locus_tag="VI935_05160"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25021.1"
                     /translation="MSDEGKKNKISPVLSDSRSLKTRSSGLAKRGLELLSSRRERVIH
                     FPTDRSMGTVYALDLDNPDSEWEELGEAKGIITVPMRMFVDLTVSEEAVIDLSPLSKL
                     QPNDLQALCFIFTDLTDAEIVHIQGLIGLIRLELRGTKITNAGLVNLQKLSGLKSLYL
                     SWSWNRITDDGLKYLEKFSELEYLHLSRAMITDVGLAYISKLTKLQSLNLILPLITDA
                     GLIHFQKLTGLKELRLFEIPITDLGMVYLRELKGLKILALGQSYLSSASLYYIRKDLR
                     ELGRVIPEITQITDTGLAHLRELSELEVLCLRKTKITDYGLVNLHSLTGLNVLDLSFN
                     LITNIGLRLLPKITDLQRLFLVGTKVTDAGLAHLQELPNLEWMDLSKTQITDAGLVHL
                     QKLSNLQQLDLSHTAVTDSGVTALKRALPNCTIKL"
     gene            complement(2611..3396)
                     /locus_tag="VI935_05165"
     CDS             complement(2611..3396)
                     /locus_tag="VI935_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HLE25022.1"
                     /translation="MGTKITRRQTPEEKELHKKLSELASLEVELAQRELDLATLQAEL
                     RAFENRYLRIVGVRYTKLDEIEAQIAEALARLNPKDSKAKKQAAEARTKAQESAQATG
                     NIQEQKEPLRFKPSESLKKLYREVAKRIHPDLTTDEEERTRRQHLMAEANRAYEEGDE
                     ERLEAILREWESSPESVKGEGPGTDLVRAIRKIAQVEERLPTIETEITQLKESDLYKL
                     KTKVEDAEKEGRDLLADMANQLDKQIADASKHLAQVTRKGRKT"
BASE COUNT         1010 a          710 c          701 g         1188 t
ORIGIN      
        1 gtaggccgct tctaaatctt gagggatgac atgaggtcga actaaagatt ctataaattg
       61 actaattcgg ccctgaccaa ttaccctatg taatcctttg tagacttttt catctatagt
      121 aatagttagc tttttctgca tacaagcctc cttatacgtg tttaatacgt ataatataaa
      181 atctataata aaaaatacaa gcagctttgc ccattatact aagtagcaat aagccttgcc
      241 gcttttgctt attctacgat tcttgggtgg tgttactatg gagagaaggc aatagagtat
      301 ctctttaaag agcgcgctgt aaaccgtaca gaattctttt taccataagg gttttagtag
      361 gtgctacaat caagttagac atcgtctggt attttgcaga catcatgaac gggcttatgg
      421 cttttccaaa cctcgttgga ctcctcggac tttctaatac tagcacgata ggcgggacat
      481 tcctgtcccg ccaccttgac ggagttagaa aactccgtct atcgtttagt aattttcaga
      541 actctgcaat tggaaatggc atatggttgt agtggaagaa acgaaaaaat acttggagct
      601 tgagatttcg gggggatgaa ttttttaata atgagcaagg agtttgttag taaaaggacg
      661 tatcccaggt aggttgactt aaaagtttct attgacaacc cctttatttt actctattaa
      721 aattttttat aacatctagt gaggagttaa aatggaaact ttattttcta caattcaaca
      781 ttatttgccc tttcttttta cgggaatatc gctaatatct ataggaatat ccgttaaaag
      841 ctatagaatg gcgagtcgat atcagaattt tgactacgct gtgaagcttc aaactataga
      901 cgagataatc aaattcggat caccatcatc tcctgaattt aattacagca ctaaaatcga
      961 aaacaggggt caaaaaccag ttaaaattga acgtgtttac atcgactatg gctctcgcga
     1021 cgatcctaag aagcgtatta aacatgtcat agaaggacaa ttctatctca ggcctggcga
     1081 accaagagaa ataaagttga atctatctca gtctgaagta gaaagcatta ggcaaaaatt
     1141 tgaccgtcaa tgtatgtttt tcctccgtgt tctttaccac acagctgagg gaagaattgc
     1201 tgaagtcact cgctttctcg gtggctatga caaagacatg attatctttg ttgtgcagcg
     1261 tggcgaatct ttgacctgac ttcttagcct cataactttg attgtctagg caataattaa
     1321 tcaaggcttt tcatagcttt atagtgcaat ttggtagtgc acgtttcaga gcagtaacac
     1381 cggaatcagt gacagcagta tgacttaaat ccagctgttg taaattgctt aatttctgta
     1441 aatgtactaa cccagcatcc gtgatttgag ttttgctgag atccatccat tccaaattag
     1501 gtaattcttg taaatgcgca agtccagcat ctgtaacttt agtaccaact agaaataacc
     1561 tctgtaaatc cgttattttt ggaagaagcc tcaatccaat attagttatt aggttaaacg
     1621 atagatcaag aacatttaat ccggttaggg aatgcagatt tacaagacca taatcagtaa
     1681 ttttagtttt tcggagacat agtacttcta actctgatag ctctcgtagg tgcgctaacc
     1741 cagtatcagt gatttgagtt atttcgggaa ttactcttcc caattccctt aaatctttcc
     1801 taatataata gagtgaggcg gatgagagat aactttgacc aagagcaagt atctttaacc
     1861 cttttagctc tcttaagtag accattccaa ggtcagtgat tggaatttca aagagtctta
     1921 attccttcag tccagtcaat ttctgaaaat gaattaatcc ggcgtcagtg ataagaggaa
     1981 gtataagatt tagcgattgt agtttagtta atttagagat gtatgcaaga cctacatctg
     2041 ttatcatggc tctagaaaga tgaagatatt ccaattcaga aaatttctct aggtatttta
     2101 atccatcgtc tgtaattcga ttccaactcc atgagagata cagcgatttc aatccagata
     2161 atttctggag gtttactaat ccagcattgg tgattttagt tccccttagt tctagcctta
     2221 ttagaccaat taatccctga atgtgcacaa tctcggcatc tgtcaagtca gtgaagataa
     2281 aacaaagtgc ttgtaggtca tttggttgta gtttagaaag aggagaaaga tcaatcacag
     2341 cttcttcact gactgtaaga tctacaaaca ttcgcatagg aactgtaatt attccttttg
     2401 cctctccaag ctcctcccac tctgagtcgg ggttatctaa atccaacgca taaactgtgc
     2461 ccattgagcg atctgttggg aaatgtataa ctctttctcg ccgagatgaa aggagttcta
     2521 aacctctttt tgctagtcct gatgaacgtg tttttaggct tctactatca gataacacag
     2581 ggctaatttt gtttttcttc ccttcatcgc tcatgttttt cttccctttc tggttacttg
     2641 agccaaatgc ttgcttgcat cggcaatctg tttatcaagt tggttagcca tgtctgcaag
     2701 gaggtctcgc ccttcttttt ccgcatcttc tacttttgtt ttcaatttat aaaggtcgga
     2761 ttctttgagt tgagtaattt cggtttcgat agtgggaagt ctttcctcaa cctgggcaat
     2821 cttccgtata gcacgaacca aatcggttcc gggtccctca cctttgaccg attcgggact
     2881 gctttcccat tctcgaagta ttgcttccaa gcgttcttca tccccctctt cgtatgcacg
     2941 attagcctct gccattagat gttgacgacg agtgcgttct tcttcatcag ttgtaaggtc
     3001 tggatggatg cgtttagcaa cttcccgata taacttcttt aaactttccg agggcttaaa
     3061 cctaagaggt tctttttgct cttgtatgtt accagtagct tgtgcggatt cttgcgcttt
     3121 agtacgggct tcggctgcct gcttttttgc cttgctatct ttgggattaa gacgcgctaa
     3181 agcttcagct atttgtgctt caatttcatc aagtttagta tagcgaacac ccacgatacg
     3241 aagatagcga ttctcaaagg cacgtaactc agcctgaagc gttgccagat ccaactcacg
     3301 ctgggctagt tctacttcga gtgaggctag ctcagatagc tttttgtgta gttctttttc
     3361 ttcaggtgtt tgcctccgtg tgattttagt tcccatacta tccaaatttg tattcccttt
     3421 tccatatctt gtcaatcggt tttttttgaa ggaaggctag gaaatagcaa attcgtacat
     3481 tggaaaaagc atattggtaa aaaaatctgt ttgacgtgat atagctgtca gtatatcatt
     3541 tctgatacag gaaaccatga tatgagcaag accaaaccta tatcgagcgt aagtattgat
     3601 aggtatgta
//
LOCUS       DATDRL010000143         6316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2584053, whole genome shotgun sequence.
ACCESSION   DATDRL010000143 DATDRL010000000
VERSION     DATDRL010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2584053"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..339)
                     /gene="groES"
                     /locus_tag="VI935_05170"
     CDS             complement(49..339)
                     /gene="groES"
                     /locus_tag="VI935_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HLE25023.1"
                     /translation="MNVRPLHDKILIKRIDAPQMTKGGIVIPDTVKEKPQEGKVVATG
                     KGRILEDGKVVPLVVKRGDRILFGKYGGTEIKIEGEDYIILDENEVLAIIEE"
     gene            complement(549..782)
                     /locus_tag="VI935_05175"
     CDS             complement(549..782)
                     /locus_tag="VI935_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25024.1"
                     /translation="MDKQFNGYTAIQTAEGFHVVFHDSSRTPLDVKFNDFQEFANKYS
                     QEILAGKLPKLSEQEAVLLSLWELMLVPANTIH"
     gene            complement(1075..1515)
                     /locus_tag="VI935_05180"
     CDS             complement(1075..1515)
                     /locus_tag="VI935_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25025.1"
                     /translation="MVALPLLTGAVESAGFGVSANGNVIVGVSILPDENFEATLWNDT
                     GAVGLGTLPGQTVSSALNASMDGSGVVGHSSECNGCATVAFIWDEENGMRNLRDVLVS
                     EGLSEELEGWALLEASAISHNGHLITGIAINPDGINEAFIATLP"
     gene            complement(1531..2223)
                     /locus_tag="VI935_05185"
     CDS             complement(1531..2223)
                     /locus_tag="VI935_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25026.1"
                     /translation="MLDPNADKIEIPIIRRSNTGNLHFRIGVCILLIFLTESLFTGCD
                     IGSGERFKASFLGLGTLSGFMASRGLGISGNGLVVVGSSFTSNTDLEAFRWTEQSGMI
                     GLGTLPDTTGSCAEAASIDGSVIAGSSTFFDVFVAFRWTAAKGLERLTPMDEEIQANT
                     ASGISADGTVIVGGVGGALTSKAYRWTEANGLVTLGTLGGDTSCAEGVSADGNVITGS
                     SSNEMGAVLAFR"
     gene            complement(3247..3606)
                     /locus_tag="VI935_05190"
     CDS             complement(3247..3606)
                     /locus_tag="VI935_05190"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HLE25027.1"
                     /translation="MIDKKSLGQKGEDIASKVLKKDGYRIIEKNFRCKQGEIDIIAEN
                     KGVVCFIEVKARSSENFGLPEEAVTSWKQRRLLAAAYVYLEKKKIKSKDLRFDIVSVD
                     LNNEKARILKNAFEVNY"
     gene            complement(3887..>6316)
                     /gene="mutS"
                     /locus_tag="VI935_05195"
     CDS             complement(3887..>6316)
                     /gene="mutS"
                     /locus_tag="VI935_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HLE25028.1"
                     /translation="KSEKNSVPLCGVPYHSAEPYVAKLLQHGHKVAICEQVEDPKFAK
                     GIVERKVVRILTPGVILDTEKLDSKSNNFLASIYFDGKSYGFSYADISTGEFRTTSFS
                     TLDELLDEVAHLEPKEILLTEELYQNDGLSSFFSRSWNPLLTKLDSYIWDLDRAKELL
                     EEKLASKTLEAFGLENHPESVAACGALVHYLKETQIEDMPPLEDPLYYQTTNYLLVDE
                     STKRNLEILRTIRSESESGSLLWVLDETLTAMGGRLLKKWINYPLIDIEKIRERLNAV
                     EELKEKPSLRRMVRGALKEVSDIERLIGRISTTAARPRDLGALRDSSSFISNIKGILS
                     DFQFTLLARIRDVLDDLADIRELLEGSLVDEPPISSRDGGIIKDGFNSEVDELRVIKR
                     DGKKWIAGLESKERKLTGVNSLKVGYNQVFGYYIEVTKPNLHLVPENYIRKQTLVNAE
                     RFVTPELKEYEEKILGAEEKILELERYLFDEIRERVAKESERIRQTASLIAELDVLSS
                     LAEVADKYNYTKPEVSDSGIIDLKESRHPVVERMDLGERFVSNDITLDTEENQFLIIT
                     GPNMAGKSTLIRQVALIVLMAQIGSFIPAREGRIGIVDRIFTRVGATDNLARGRSTFM
                     VEMVETAYILRHATQRSLVVLDEIGRGTSTFDGMSIAWAVGEFLHDLGCRSLFATHYH
                     ELAQLAITKRRVRNYNVFVKEDKGKVVFLRKLIPGAASHSYGIQVARLAGVPEKVLKT
                     AREVLFKLEKAESNLGSGIRGGQLGLFEKKEDEIGDEILAEINKLDPNSMTPLEALSK
                     LMEIKGKLDNS"
BASE COUNT         1737 a         1536 c         1156 g         1887 t
ORIGIN      
        1 ccaaatgcaa tctgtttagc agccatttgt taattcctcc ttgaaaattt attcttcgat
       61 tatagccaat acctcatttt catctaggat tatataatcc tcaccctcga ttttaatctc
      121 tgttccccca tatttgccga acaatatcct atcacccctc ttcaccacta agggaacaac
      181 ctttccatcc tctagaatcc tacctttacc agtagctact acctttccct cctgtggctt
      241 ttctttgaca gtatccggaa tgacaatccc gccctttgtc atctgggggg cgtctattct
      301 tttgattaaa atcttatcgt gaagtggtct tacattcatg gttttacctc ctggattggt
      361 atataaatgg gattagcact ctagttagtt gagtgctgag tataagatac gcatcaatcc
      421 cgcaggtgtc aagaggaata atgacgcagg atgctatcta tttgaaaact tattcaagaa
      481 agggttggtt taaataggga tgacctaaat cctgatcccc tatggataga atcacggatc
      541 tcactacctt aatgtattgt gttagctggt acaagcatta actcccatag cgaaagcaaa
      601 actgcttctt gttcagagag ttttggaagt tttccagcca gtatttcctg gctatattta
      661 ttagcgaatt cttgaaaatc gttaaatttt acatctagtg gggttctaga agaatcgtgg
      721 aacaccacat ggaaaccctc cgcagtttgt atagctgtat atccgttaaa ttgtttgtcc
      781 ataattaaac accttaactt tcaatataat cattaactat tcgatttaaa gtggaaattt
      841 tttaatctcg ctcgtactga taaaaaatcc tcgatacagg atgcacactt aaattatgcg
      901 cgcacacttc tagattttgg tagggtttgg gtttgcacaa tggcacaata accaagatag
      961 ttaatgaaaa aaaatcacaa tccaaggcct gaatgaaatt aaatgaaagg aatttttgtt
     1021 gccacttgac agggactggc taatgggtga cccgattcca ccgggattag gattttatgg
     1081 caaggtagcg atgaatgcct cgttaatccc gtcgggatta attgcgatac cagtgattaa
     1141 atgtccgtta tgcgatatcg cactcgcctc cagcaaagcc catccctcta gttcttcact
     1201 caagccctcg cttacgagga catcgcgtag gtttcgcatg ccgttttcct catcccaaat
     1261 gaaagcaacg gtggcgcagc cattacactc gcttgaatga ccaactaccc ctgatccgtc
     1321 catagatgca tttagtgcac tacttaccgt ttggcccggt agggttccta ggcccactgc
     1381 accggtgtca ttccataggg ttgcctcaaa attctcatct ggtaaaatgc taactcctac
     1441 aataacattt ccattcgcag agacaccaaa cccagcactc tctaccgcac ctgtcaacag
     1501 gggtagtgcg accattcccg tttcggaggt ctaccggaat gccaagactg ctcccatttc
     1561 gttactgctg gagccggtta tcacgttgcc atccgccgag acaccttcgg cgcaactcgt
     1621 gtctccaccg agggttccaa gggtcaccag cccattagcc tctgtccatc tataggcctt
     1681 ggaggttaag gcaccaccta ccccaccgac aatcacggtt ccatctgcag agattcctga
     1741 cgctgtatta gcttgaattt cctcgtccat cggggtaaga cgttctaatc cctttgctgc
     1801 tgtccatcga aatgccacga aaacatcaaa aaacgtactg ctccctgcaa taactgaacc
     1861 atcgatgctg gccgcttctg cgcagctacc ggtagtgtca ggtagggtgc caagcccaat
     1921 cataccgctc tgttccgtcc aacgaaaagc ttctaaatct gtattcgaag taaagctgct
     1981 acccacgacc acaaggccat tgccagagat gcccaaacct cgtgaagcca taaacccaga
     2041 taatgtacca agtcccagaa agctagcttt gaagcgttcc ccagatccga tgtcacaccc
     2101 tgtgaacaag ctttcagtca agaatatcaa aagaatgcaa acgccgattc gaaaatggag
     2161 attgcccgta ttggacctcc taattattgg aatttcaatt ttgtctgcat tggggtcaag
     2221 cattttgttg acaaattata ccctagatcc cttacacctt ttttctataa tttaacttaa
     2281 aagggctgcg tctgtacata ctttcattct tcttttctat tattaccgca cccagcctac
     2341 tgactcatac cggtggtcgt cacctagata tggccacctt ccgccagaaa cggcgagcca
     2401 ggcacgggcg ttaacgcccc agtcaaagca ttgatcgaga aaaaatagcg tcaggtcttg
     2461 aaatataagg ttttatataa acaaatcggg gtcaggtctc aacatttgac ataaggagaa
     2521 aatgaaagtt aaaagttgag acgcgacccc atttatgctt tacacacggc aacaagggtt
     2581 tcacgggtgc tgcgctgcga ttcgaccaca taggtgaagt cttcgagttg gcatgttctc
     2641 tgaccccttt acagaatctt ttccgcataa tcaccctata cgagtggtat gaggaaattg
     2701 ttgcaatgtt aattcctctt accatatttc attatatcac ttagttttta atcaacacaa
     2761 ccttttttat ctaacgaaag aaattaaaca aaaggacaga aatggggcca ggacttgaaa
     2821 tataagattt tatctgttca aatcctctag actagtaccc aagaattgaa tagccaagta
     2881 aaacacattt ttgagattcg gacgactctg gggttgtact atactgcgac aatctcgatc
     2941 aagaaaaatg cacgaatcaa taaccctgca gcaagctgca gggtatggtc taaatattaa
     3001 acgccgcaac tgtcattgcg aagaaccctg agcaaagtcg aaggggacga agcaatctaa
     3061 attagattcc tcacttcgga atgacacttt gtgtcagatt gcttcacgga gtttatcgat
     3121 aggcggggtt ttcaaacccc gcatcctgtt gcgggacaag aatgtcccgc ctatccgctg
     3181 caggctcagt gaatgtgttc gcaatgacaa tacagcattg tggaaaccct gtgccctctt
     3241 ctcaacctaa taattcacct caaaagcatt cttcaagatt ctagcctttt cattgttaag
     3301 atcaactgac actatgtcaa agcgcaaatc ttttgatttg attttctttt tttccagata
     3361 aacataagcc gcagccagga gtcttcgctg tttccaactt gtaacggctt cttctggcag
     3421 tccaaagttc tcagatgaac gggccttaac ctctatgaag caaacaacac ctttattctc
     3481 agcgattata tctatctcac cctgcttgca cctaaagttc ttttcgataa tacgataccc
     3541 atcttttttt agaaccttgg atgcgatgtc ctcgcccttt tgtccaagtg attttttatc
     3601 aatcatgggt tactttctag ccacgaataa acacgaattt acacaaataa aataatacat
     3661 agaaagctaa tggtctttga aatattaatt tggtaataga gaacctaaat gggtaggcaa
     3721 cgggtcttgc tcgcttccct ctagtccacc agcaagactg gttggctacc atacgtaggt
     3781 gtctaatatc caacttaaag tcttcaaaaa ccattagggt tccattagaa taaaatttta
     3841 cttgcctttt cattcgtgct tattcgtgtc attcgtggcc gaataattag ctattatcta
     3901 actttccctt aatctccatg agcttagaaa gtgcttcgag cggggtcata gaattaggat
     3961 cgagtttgtt aatctccgct aaaatttcat cacctatttc atcttccttc ttctcaaaaa
     4021 gtccaagttg tccaccacga ataccacttc caagattaga ctccgctttc tcaagcttga
     4081 agagtacctc gcgtgccgtt ttaagaacct tttctggtac ccctgctagc cttgctacct
     4141 gaattccata actatggcta gcagcgccgg gaattagctt tcttagaaac acaacctttc
     4201 ccttatcctc ttttacaaag acgttgtagt tcctaaccct tctttttgta atggcaagct
     4261 gggcaagctc atgataatgc gtagcaaata gggatctaca ccctaaatcg tggaggaatt
     4321 ctcctactgc ccacgcaata ctcatcccgt caaatgtgct tgttcccctc cctatctcat
     4381 caagcactac tagacttctt tgcgtggcat gtctcaggat gtaagcagtc tcaaccatct
     4441 ctaccatgaa cgttgatcgt cctcttgcta aattatcagt agctccaacg cgggtgaata
     4501 tcctatctac aattccaatc cttccctccc ttgctggaat aaagcttcca atctgagcca
     4561 taagaactat tagcgcaacc tgacgaatga gagtggactt cccggccata ttaggaccag
     4621 ttattataag aaactggttt tcttctgtgt ctagcgttat gtcattcgat acaaaccttt
     4681 cacctagatc catcctttct acaacaggat gccggctttc cttaaggtca ataatccctg
     4741 agtcgcttac ttcgggttta gtgtagttgt atttatcggc aacttctgcg agagaagaaa
     4801 ggacatcaag ctcagctatc agcgaagcag tctgtcttat tctctctgat tccttcgcta
     4861 ccctttccct tatctcgtca aaaagatacc tttcaagttc tagaatcttc tcttcagctc
     4921 caagaatctt ttcttcatac tccttaagct ccggcgtgac aaacctctcc gcatttacta
     4981 gtgtctgctt tctaatataa ttctccggaa ctagatgaag gtttggtttt gtaacctcaa
     5041 tgtagtaacc gaatacttga ttgtatccga ccttaagtga gttgactcct gttaatttcc
     5101 tctctttcga ctcaagccct gctatccact tctttccgtc acgctttata accctaagct
     5161 catcaacctc actgttaaaa ccgtccttaa ttatccctcc atcacgagaa gaaataggag
     5221 gctcatcaac taacgaaccc tctagtagtt cccttatatc agctaaatcg tcaagaacat
     5281 ccctaattct agcaagtagt gtgaactgaa agtcgctcag gatacctttt atattagaga
     5341 taaaacttga tgaatccctg agtgctccta gatcccttgg ccttgcggct gtggtagata
     5401 ttctcccaat cagcctttct atgtcactta cctcttttaa ggcaccccta accattctcc
     5461 taaggcttgg tttttctttg agttcttcaa cagcattcag cctctctcta atcttttcta
     5521 tatctataag cggatagttt atccacttct taaggagtct ccctcccatt gccgttaggg
     5581 tctcgtcaag aacccataaa agagaaccgc tttctgattc actccgaata gttctaagga
     5641 tctcaaggtt tctctttgta gattcgtcaa ctaataaata attagtggtt tgataatata
     5701 atggatcctc taatggaggc atatcttcta tctgggtttc tttgagataa tgaacaagtg
     5761 ccccacaggc ggcaaccgat tccgggtgat tctcaagtcc aaaggcttct aatgttttcg
     5821 aggctaattt ttcctctaaa agctctttcg ctctatccaa atcccagatg tatgaatcga
     5881 gttttgtaag aagaggattc caactacgtg aaaaaaagga agacagtcca tcattttgat
     5941 aaagttcttc tgtaagaaga atctctttag gctcaagatg agcgacttcg tctaagagtt
     6001 catcaagggt agagaacgaa gtagtcctga actctccggt agatatatcg gcatatgaaa
     6061 aaccgtaaga tttcccatcg aagtatatac ttgcaaggaa attatttgat ttagaatcga
     6121 gcttctccgt atcaagaatt actccaggtg taagaattct gacaaccttc ctttcaacaa
     6181 tcccctttgc aaacttaggg tcctctacct gttcgcatat agctacctta tgaccgtgtt
     6241 gaaggagttt cgccacgtaa ggctcagcgc tatggtatgg aacaccacat agtggaacag
     6301 aatttttctc agattt
//
LOCUS       DATDRL010000144         8703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2156046, whole genome shotgun sequence.
ACCESSION   DATDRL010000144 DATDRL010000000
VERSION     DATDRL010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8703
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2156046"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..794
                     /locus_tag="VI935_05200"
     CDS             123..794
                     /locus_tag="VI935_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HLE25029.1"
                     /translation="MDSNYFRLLLIQVPVILFALTVHEFCHAWVAHLLGDDTAKRQGR
                     LTLNPIAHLDVLGTILMFVAGFGWAKPVPVNPLNFKNPRKGMLLVALAGPLSNLIMAI
                     AAGVAIRFTIPGLNLPLSGSMSVSLVLLILTLQFGLALAIFNMIPIVPLDGSRVLYGL
                     LPEEQAYEYRRFEPYGILILFALFVFGDRIFAYILWYPVSVLTEALSGYNYFQLTHLW
                     RSLAL"
     gene            791..1813
                     /gene="trpS"
                     /locus_tag="VI935_05205"
     CDS             791..1813
                     /gene="trpS"
                     /locus_tag="VI935_05205"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HLE25030.1"
                     /translation="MKSIVSDVKSTSDRNNRILSGMRPTGKLHLGHLHGALENWRELQ
                     NRFECFYFVADWHALTTDYADPSGIREATFEMLADWIACGVDPNKATIFIQSHIKEHA
                     ELTLLLAMITPLSWLERVPTYKEMKEELRDKDLATYGFLGYPLLQTADIIVYKANYVP
                     VGEDQVPHLELGREIVRRFNYLYGDTFPEPQPLLTKVPKILGTDGRKMSKSYGNAIYL
                     SDSPAEITEKIMKMYTDPQRLRRSDPGNPDVCGIFYLHKYYSSEDDISMVNRECRTAG
                     IGCVDCKKLLLPSLINELAPIREKREKLMRKKHDLEEVIQEGDKKAREVAKETLEEVR
                     KKVGLN"
     gene            1800..2558
                     /locus_tag="VI935_05210"
     CDS             1800..2558
                     /locus_tag="VI935_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HLE25031.1"
                     /translation="MVSTEPCLIKLHAFEGPLDLLLYLIKKNEINIYDIPIAVITEQY
                     LMYLEVMRELNLEIVGDYLMIAAELGLIKSKMLLPKPEVEEEEDPRAELVRRLLEYQR
                     YKEAAGTLLAFDILERDVFTRIIQDEDDSDEINQPVRTDLWSLIEAFRAIWNRKDFSS
                     ISNISLELELITLEQKVSELITKLKIHKNILFEELFGENPTRFEIVITFLAVLELIKS
                     GLLIAYQDFSYSPIQILYVGDNPTYYSKVVDEIE"
     gene            2564..3118
                     /gene="scpB"
                     /locus_tag="VI935_05215"
     CDS             2564..3118
                     /gene="scpB"
                     /locus_tag="VI935_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HLE25032.1"
                     /translation="MEKNNIKRAIETIIFVSERPVSSKRLLQVFQDLKKDEIQKYLDE
                     LVDDWNALNRGFKLHEVANSYQFRTNPEHSEIIMRFKQSKPFRLSRAALEVLAITAYK
                     QPVTRIEIDEIRGVDSSGVIGLLLEKRLIEIKGRKEVIGRPFLYGTTEVFLETFGLKS
                     LVDLPTLKELVEIEKSLEESAKVY"
     gene            complement(3101..3529)
                     /gene="ybeY"
                     /locus_tag="VI935_05220"
     CDS             complement(3101..3529)
                     /gene="ybeY"
                     /locus_tag="VI935_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HLE25033.1"
                     /translation="MKAHIVDYVDFLNKEEKRKLRNHIKLILKHLRLASNKDLCITFL
                     DDKGMKELNSTYRGIKRTTDVLSFPQDGPDIRSLGDIIISADTAKKHARFYKISLQRE
                     ITKLIIHGILHILGYDHKKKNDANKMRKKEKELLQLVNFS"
     gene            4436..5776
                     /gene="der"
                     /locus_tag="VI935_05225"
     CDS             4436..5776
                     /gene="der"
                     /locus_tag="VI935_05225"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HLE25034.1"
                     /translation="MPTETNKMPLVAIVGRPNVGKSTLFNRIIGWQKAIVEETPGVTR
                     DRLYAVTEWSGVTFRIVDTGGFEPVSEDLYLSLIRNQVQKAVEEADLILFLLDAKTGL
                     MPQDIEISKLLRKVKKPVLYVVNKVDHEKQETNTMEMHALGIETFILISSLHGRRINE
                     LLDVIVEKLPEAKLKVEEEDEGIIKVAVIGKPNVGKSTFVNRILGEERMITSPIPGTT
                     RDSIDTLIERDGKKYMFIDTGGIRKKSKITMPVERYSTLRAIKTIERADIILLMLDAS
                     EGPTHQDARLAELINERNKGCIILINKWDLAPREIAQRKGIQDIIRSKLKALEFAPVI
                     LISALTGKRVIKVFDYIDLAYKNFSCRIRTKPLNKLLENILRTNPPPLYKGSEIKFYY
                     MSQPLTKPPTFIVFTNFLKGIPDSYKRYLENRLREEFELQGTAIKVIYRGRRKS"
     gene            complement(5796..7076)
                     /locus_tag="VI935_05230"
     CDS             complement(5796..7076)
                     /locus_tag="VI935_05230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLE25035.1"
                     /translation="MRAQLRTLFINHSVRDGGPGRSLFYILKYIDKTKITPFVLVPRE
                     DVFTELVNKEGLGDIIVVEKRFPENILRPRFNANWRSQPPWLEGQASSPKPNKTPRNI
                     ISIAVQIISISLNVFDILLLILKSPFLLKKWNIDVIYCNGTLAKIVGVLIGSLNSRSV
                     IWHVRNIQRTKSLRLTMNLLSLLPAVKRIICVSNATAKQFRVMRNKISVIYNGVDFEE
                     FNPEKVPGDLRLKYGIPEGVVIIGSTGRIVPRKGYKNLIEAALTVCKELGEGEINKIR
                     FVIVGDTPYFFQDNHLQNLKSLIKEYKLEGIFIFTGYQRDVKPYLKDFDIFVIPSSYE
                     DPFPRVIIEAMAFALPVIGFSVGGIAEAVEQGATGILSESGNTEQMGESIFKLILDKS
                     LRHSMGVAGRERTKRLYSAKTRTKDIENIILELK"
     gene            7480..8160
                     /locus_tag="VI935_05235"
     CDS             7480..8160
                     /locus_tag="VI935_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25036.1"
                     /translation="MRIKNIVPISIYIICIITISIFTSGGCPIEFGGGGGDGDGGNLV
                     SIVQGTIISTPNSGITAVLINNENGTVFESNPTDNSGNFFITGDFNGCLTLRLEFQDS
                     NEDVIAFTNVTVFPKTNLDLGDVSLDNGNVILDDPDNISVNFDGKINFTDAPRTCFGG
                     VNDSQGTIVITRSNTDITVQIVPTTNIEDTNNNPIGCDNLIADAEVEVTGTLQVHDTV
                     LADIITLK"
     gene            complement(8327..>8703)
                     /locus_tag="VI935_05240"
     CDS             complement(8327..>8703)
                     /locus_tag="VI935_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25037.1"
                     /translation="FLHMLIKYGNRMARDFFENYFTIMFQPYLSIQEGMKKNLQLWQE
                     TGWAPPGIRYPVIGNHFETVEGHPKKQNNIEKEGGTKESSHTPPSAELELLMEKVKEL
                     EDKVKSMDKPKRGNRVKEKVTE"
BASE COUNT         2826 a         1511 c         1674 g         2692 t
ORIGIN      
        1 tcataaaata ttggtatctg ataatttggc taattcaaaa gtcaatatca aaaagcctaa
       61 acttggatat cagacgctag gtagtgttaa tttgagagcg tacataataa gataataaat
      121 atatggattc taattatttt agacttttat taattcaagt tcctgttatc ctctttgctc
      181 ttactgtgca tgagttctgt cacgcttggg ttgcacattt gcttggggat gataccgcaa
      241 aaagacaagg tcgattgacc ttaaatccta tcgcgcacct agacgtgcta ggaacaatac
      301 taatgttcgt agcaggcttc gggtgggcta agcccgtccc agtaaatcct ctaaatttta
      361 agaacccaag aaaggggatg ctcttagtag cccttgccgg cccattatct aacttaataa
      421 tggcaattgc tgccggggta gcaattaggt ttacaatacc aggtttaaac cttccattat
      481 ctggatcaat gtctgtgtca cttgtactcc tgatactaac actccaattc ggccttgctc
      541 ttgctatatt taatatgatt ccaatagtcc cactcgacgg ttctagggta ctctatggtc
      601 ttctgcctga ggaacaggca tatgaatata gacgatttga gccttatggt atattaattt
      661 tgtttgctct ttttgtcttt ggtgatcgaa tcttcgcata tattctatgg tacccggtct
      721 cggttcttac tgaggcgttg tcagggtata attactttca actcacacat ctttggaggt
      781 cactcgcttt atgaaatcca tagtatctga tgtgaaatcc acatcagata ggaataacag
      841 aattcttagc gggatgcgac ctacaggaaa gctacatctt ggtcacctcc acggtgcctt
      901 agagaattgg cgtgagcttc aaaatagatt tgaatgtttc tatttcgttg ctgattggca
      961 cgcccttaca actgattacg cagatccatc tggaataagg gaagctacct ttgagatgct
     1021 tgctgattgg atagcgtgtg gggttgaccc aaacaaggct acaatcttca ttcaatcaca
     1081 tataaaagag catgctgagt taaccttact cctggcaatg ataacaccac taagctggct
     1141 agagcgagtt cccacgtata aagagatgaa ggaagagctt cgggataaag accttgccac
     1201 ttatggtttt ctggggtatc ccctactaca gacagcggat ataatagttt ataaggccaa
     1261 ttacgtacct gttggagagg accaggtacc acatctagag cttggtagag aaatcgtaag
     1321 gcggttcaat tatctatatg gtgacacatt cccagaacct caacctcttc ttacaaaggt
     1381 tccaaagatt ctcgggactg atggaagaaa gatgagtaag agttatggta atgctattta
     1441 tctaagtgat tcccctgctg aaataacgga aaagataatg aagatgtata ctgacccaca
     1501 aagacttagg agatctgatc ccggaaatcc ggatgtctgt ggtatttttt acctccataa
     1561 atactatagt tctgaggatg atatatctat ggttaataga gaatgtcgaa ccgctgggat
     1621 tggttgcgtt gattgtaaaa aactacttct accaagctta attaatgaac tagcaccaat
     1681 tcgagagaag cgagaaaaac ttatgaggaa aaagcatgat ttagaagaag taattcaaga
     1741 aggtgataaa aaggcaagag aagtagctaa agaaaccctc gaggaggtaa ggaaaaaggt
     1801 tggtctcaac tgaaccctgt ttaataaaat tacatgcgtt tgaaggacca ctggatcttc
     1861 ttctttacct cataaaaaag aatgagatca atatttatga tatcccgatt gcggtcataa
     1921 ctgagcagta tcttatgtac ctagaggtga tgcgcgagct taacctcgag atcgtagggg
     1981 attatcttat gattgccgct gagctagggc ttataaagtc aaagatgctt cttccaaaac
     2041 ccgaagttga ggaggaggag gatccaaggg ctgaacttgt aagaaggcta ctcgaatacc
     2101 aaagatataa ggaggctgca gggacgcttc tagcttttga tatattagaa cgagatgtat
     2161 ttacaagaat cattcaagat gaagatgatt ctgatgaaat aaatcaacca gttagaacag
     2221 atttgtggtc attgattgaa gcctttcgag cgatctggaa tcgaaaggat tttagttcga
     2281 tcagtaatat tagcttggag ttagagttaa taacgcttga gcaaaaggta tcagagttaa
     2341 ttacaaagct taaaatacat aaaaatatct tatttgaaga actttttggt gaaaatccaa
     2401 caaggttcga aattgtaatt acatttcttg cagttttaga gcttataaaa tctggattgc
     2461 taatagcata tcaggatttc tcctattctc ctatacaaat attgtatgta ggggataacc
     2521 caacatatta ctctaaagtc gtagatgaaa tagaatagca gtgatggaaa aaaacaatat
     2581 aaaaagggca atcgagacta taatcttcgt ctcagaacga cctgtatctt ccaaaagact
     2641 tctccaggtt tttcaagatc taaaaaagga tgaaatacag aaatacctcg atgaattagt
     2701 agacgattgg aatgctctta atcgtggatt caaattgcac gaggttgcaa atagttacca
     2761 atttagaaca aatcctgaac atagtgaaat tataatgcga tttaagcaat caaagccatt
     2821 tcgcttaagt cgagctgcat tagaggtact tgctataacc gcctataaac agcctgttac
     2881 aaggattgaa atagatgaga taagaggagt ggactcttct ggtgttatag gattacttct
     2941 tgaaaaacgt cttatagaaa taaaaggtag aaaagaagtc attggaagac cattccttta
     3001 tgggactact gaggtatttc ttgaaacatt tggacttaaa agtctcgtgg atcttccaac
     3061 tcttaaagag cttgtagaaa tagaaaaatc actcgaagag tcagctaaag tttactagct
     3121 gtaatagttc cttttctttt tttctcattt tattagcatc attctttttc ttatggtcat
     3181 aacctagaat atgaaggatt ccatgtatga tgagcttcgt tatctctctt tgtaggctta
     3241 ttttatagaa tcgtgcgtgt ttctttgctg tatcagcaga tatgattata tccccaagtg
     3301 atcttatatc cggaccatct tgcgggaaag aaagtacatc tgtcgttctc tttattcccc
     3361 ggtaggttga attaagttcc ttcatgccct tgtcatctag aaaagttatg cagaggtctt
     3421 tattactagc aagcctcaga tgcttaagaa ttagtttaat gtggtttctt aattttcttt
     3481 tttcttcctt gttaagaaaa tcaacataat caactatatg tgctttcatc tttactttag
     3541 atcacatgta cttgcttatt acatatgaga aaaatgagag ggttaattga tatggataat
     3601 gccattatct taaataaatt gcaacggtgt cttgtacggt gagatttatt gcaagagtcc
     3661 gtatttcacc tgtacattgg ccttgagtgg tataatctat tctagctaag gaatgggttg
     3721 attcttgtgt cacggtagtg tttaaaaacg gcctaccctg atctggatta cagccgtaaa
     3781 tataggtcac ctctacactc aagccatcgg caccaatgaa cccgacagat tcaaagtgcg
     3841 gccatctgat tgcaaaccca gaggccggtg gagcctaaca ggacagaaat aatagccacg
     3901 attgtgaagg tcagttttaa tacaaaacgc tcccagggtt tggctgcttt cttcatgagt
     3961 ggagggttga tttgaattca ttacatcctc ctttcgttcc cgcattatta tgatgtaatt
     4021 attttttatc ctttataacc cagattatgc ttcttacgaa tatttgttaa gtatatagga
     4081 gactacaaga cctgtgtaac agtcagttat tacgacctta ttgtatcttc aaatagatgg
     4141 gtgactaatc tgatgctaaa tttgaaatta gttatgatat catatcttgc aagtagaaga
     4201 ttttagaatt ttcttcaaga atttcttgaa aagaatatag aatttaaaaa tactactgct
     4261 atgattattt gataaattac ctatcttata ttgatggaat ataagctaga gatagtaatt
     4321 acaaaaatct acggctaaaa taaaacatag ttcataaatc cattcataaa gtataaatca
     4381 tttaaagtca gaacctttat ttagcataca aataaactgt aagaaggtta tcattatgcc
     4441 tacagaaact aataaaatgc ccctggttgc aattgttgga aggccaaacg ttggaaaatc
     4501 aactctcttc aaccgaataa tcggatggca aaaagcaatt gtagaagaaa ccccaggtgt
     4561 gacaagagat agactttatg cagttactga gtggtcgggt gtaacattta gaatagtaga
     4621 tacgggcgga tttgagcctg tttcagaaga cttgtatcta tcattgatta gaaaccaagt
     4681 tcaaaaagca gtagaagaag ctgatttgat actttttctg cttgatgcaa aaacaggatt
     4741 aatgcctcaa gacattgaaa tctcaaagct gctgagaaaa gtaaaaaaac ctgtgcttta
     4801 tgttgtaaat aaagttgatc atgaaaaaca agaaaccaat acaatggaaa tgcatgcctt
     4861 gggaatagaa acctttatcc ttatatcttc tttacacgga aggagaataa atgagctttt
     4921 agacgtgata gttgagaagt taccagaagc taagctaaaa gtcgaagagg aggatgaagg
     4981 aataataaaa gtagcggtga ttggaaaacc gaacgttggc aaatcaacct ttgttaacag
     5041 aatactgggt gaagaaagga tgattactag tcctatccca ggcacaacta gggattcaat
     5101 agatacatta atagagagag atggcaaaaa gtatatgttt attgatacgg gtggcataag
     5161 aaaaaagtca aaaataacta tgccagtgga aagatatagc accttaagag ctataaaaac
     5221 aatcgaaagg gcagacataa ttctactaat gttggatgca agcgaaggcc ccactcatca
     5281 agacgcacgt cttgctgagc ttataaatga aagaaataaa gggtgtatta tccttataaa
     5341 taaatgggat ctggctccaa gagaaatcgc acaaagaaaa ggaattcagg atataattag
     5401 aagtaaacta aaagcgttag aattcgcccc ggtaattttg atctctgccc ttacaggaaa
     5461 gcgagtcatt aaggtttttg attacattga cttagcttac aagaatttct catgtagaat
     5521 tcgaacaaaa ccacttaata aactactcga gaatatcctt agaacgaatc ctcctcccct
     5581 ttataaaggt agtgagatta aattctatta catgtctcaa ccattaacta agcctcctac
     5641 ctttatagta tttacaaact tcctaaaggg tataccagat agttataaga ggtatttgga
     5701 aaatagacta agagaagaat tcgaattaca aggaactgct attaaagtta tttatagagg
     5761 taggagaaag agctaagaat ctttattaat tagttctatt ttaactccaa tattatattt
     5821 tcaatatctt ttgtccttgt tttcgcagaa taaagtcttt tcgtcctctc cctaccagca
     5881 actcccattg aatgtctaag ggatttatct agtatgagtt taaaaataga ttcacccatt
     5941 tgctcggtat taccagattc acttagtatt cctgttgctc cttgttctac agcctcagca
     6001 atccccccta cactaaaccc tataaccggg agtgcaaaag ccattgcctc tatgataacc
     6061 ctaggaaatg ggtcttcgta gcttgatggt atgacaaaga tgtcaaaatc cttgagataa
     6121 ggtttaacgt ctctttggta tcctgtaaag ataaaaatac cttctagttt atactccttt
     6181 atcaatgact ttagattctg caggtgatta tcttgaaaaa aatatggggt atccccaaca
     6241 atcacaaacc ttattttatt gatctcacct tctcccagtt ccttacagac agtcaaggcg
     6301 gcctctatta ggttcttata accctttcta ggaacaattc tccctgtgct gcctataatg
     6361 accacaccct caggaattcc atatttgagt cttaggtcac ctggcacttt ttcgggatta
     6421 aattcctcaa aatcaacccc attgtaaatt actgatattt tatttcgcat aacacgaaac
     6481 tgttttgctg ttgcatttga aacacagata atccttttta cagcaggtaa cagggaaaga
     6541 aggttcatag taagtctaag tgattttgtc cgctgaatat tcctcacatg ccaaatgaca
     6601 gaacgggagt ttagagatcc tattaaaacc ccgactatct ttgcaagagt accgttacag
     6661 taaatcacat ctatattcca tttttttaat agaaaaggag actttagaat aagtaaaagg
     6721 atatcaaata cattcagtga tattgatata atctgaacag ctattgaaat tatgttacgc
     6781 ggagttttat ttggttttgg agatgaagct tgaccctcta accatggtgg ttggctgcgc
     6841 caatttgcat tgaacctagg tctaagtatg ttttcaggaa accttttctc aacgacaata
     6901 atgtctccta aaccttcttt gttaaccagc tcagtgaaga cgtcctctct tggaacaaga
     6961 acaaaaggtg tgatttttgt tttatcaatg tacttaagta tatagaaaag gcttctgccc
     7021 ggccctccgt ccctgaccga atggtttata aaaagtgttc taagttgtgc tctcattaat
     7081 actcctcata acccaaataa ctacgaaaaa ttacagattc atatttattt gtatgaattt
     7141 gatttccgaa atactagttt taaatccaaa aaagaaatgg gaaaccctgc aacaacctgc
     7201 agggcatcgt agtataaaca gacggccttt ttgtcattgc gaggagcaat gagacgaagc
     7261 aatctcaaaa gagagtttgc ttcactttgt tcgcaatgac tgtggaaacc ccgtagcaat
     7321 ctacggggaa ttatcgagtt aagttcgtat ttgcaaatat gaaacttttt aaaactaccc
     7381 acaactagca atttaccatg agattacaag ttaatgaaaa tttaatttat cggggtatac
     7441 ttttaataat attaaatatc caaaaaagca ggaggtaaaa tgagaattaa aaatattgta
     7501 cccatatcaa tttatatcat ctgcataata actatttcca tatttacttc aggagggtgt
     7561 ccaatagaat ttggtggtgg tggtggagat ggtgacggag gtaatttagt atcaattgtt
     7621 cagggaacaa ttatcagtac tccaaatagt ggaataacgg cagtcctaat aaataatgaa
     7681 aatggaactg tttttgaatc aaatccaaca gataatagtg gaaatttttt tatcacagga
     7741 gactttaatg gctgtttaac acttagactt gagtttcagg atagtaatga agacgttata
     7801 gcttttacca atgttacggt ttttccaaaa acaaatcttg atttaggaga tgttagcctt
     7861 gataatggga acgttattct tgatgatccg gataatataa gtgtaaattt tgatggtaaa
     7921 attaatttta cagatgctcc aagaacttgt tttggaggtg tgaatgacag ccaaggcact
     7981 atagtaataa caagaagtaa tactgatatt acagttcaaa ttgttccgac cacgaatata
     8041 gaagatacaa ataataaccc tattgggtgt gataatttaa ttgctgatgc agaggttgag
     8101 gtgactggaa cacttcaagt acatgataca gtgttagcgg atataattac actcaagtag
     8161 taatgctcag tgattgagaa ataagcgagt ttataaatct aattaatgca ttatcttgag
     8221 tataagggat atttagaagc aggtataggt acaaggcatg tgaagagtta ttagatttac
     8281 ttgatagggt tctattttat tcaaattaat tacgtaacaa ggatttctac tccgtaacct
     8341 tctccttcac tctgttgccc cttttaggtt tatccatgga tttaacctta tcttcaagct
     8401 ctttgacctt ttccataagg agctctaatt ctgctgatgg aggggtgtga gaagattctt
     8461 tagttccccc ttctttctct atattatttt gttttttagg gtgtccttcg actgtttcaa
     8521 aatggttccc tattacagga tatctaatcc cggggggggc ccaccctgtt tcctgccaca
     8581 actgaagatt ctttttcatt ccctcctgaa tagaaaggta aggctggaac attattgtga
     8641 aataattttc aaaaaaatct cgagccattc tattaccata tttgataagc atgtgtagaa
     8701 agg
//
LOCUS       DATDRL010000145        22437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3003459, whole genome shotgun sequence.
ACCESSION   DATDRL010000145 DATDRL010000000
VERSION     DATDRL010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 22437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22437
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3003459"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VI935_05245"
     CDS             complement(<1..223)
                     /locus_tag="VI935_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25038.1"
                     /translation="MFKLIVTLIQLLVNESKLLRMVLLRLGLSFLLLGAAWAFMVIGL
                     GFIVWALYLYLTNYLNPPIAALASGFLLLL"
     gene            complement(236..550)
                     /locus_tag="VI935_05250"
     CDS             complement(236..550)
                     /locus_tag="VI935_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25039.1"
                     /translation="MTERKFQEDIVTLKEDMVKLRSDMTEIAKKLIDIGKTEAKEAKE
                     KLETEARNLIDELVEELDQVSERGKKTFKTMEKEVKEKPLLSLVLAFLVGFLLGNLLN
                     RR"
     gene            814..2229
                     /locus_tag="VI935_05255"
     CDS             814..2229
                     /locus_tag="VI935_05255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLE25040.1"
                     /translation="MRRQNRFSSIGKTFLYLIFLALVIGGFIFIFPRFEWHAPTVEMK
                     LDSEFIGLRPFDVVIKDKGKGLKRVSIGLTNEQGEFPLIQEYYSLPVMEKNFAIALDP
                     KKLGIKDGPATIRVTAVDGSYWGFFKGNKTTLSKDVKIDVTPPRVEVLSRENYINFGG
                     SGAVIYKTSKDSVKNGVKIGDYFFKGYKGYFNNPDVYIAIFAYPYNVPTDKKIVVIAE
                     DGAGNSKEVGFSYRLKDVKYRKSSLDISDDFIERKVVPLLGDDSNQGDDLKGSFLKVN
                     RELRKKNEDEIKRIGEKSSSSILWSGGFHQLTNSKVEANFADARTYIYNEEVIDNQYH
                     LGYDLAVTQKYPIEAANDGVVLFAGDLGIYGNTVIIDHGLGVATLYGHMSSIDVKVGD
                     KIKKKQIIGRTGDTGLAAGDHLHYAVLLDGVPVLPIEWWDEKWINDNILNKIKEAELN
                     FGTRSTQGDISEESPSQPKVQ"
     gene            2232..2984
                     /gene="rlmB"
                     /locus_tag="VI935_05260"
     CDS             2232..2984
                     /gene="rlmB"
                     /locus_tag="VI935_05260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HLE25041.1"
                     /translation="MIIYGKNPVKEAISQTLSKIEEILISQELEKERISDIIRLAREK
                     NVKVSFLPRDAISRIAGTSHHQGIAARVSEFEYNNVDNILGNAKQKGERLLIILLDHI
                     EDPQNLGAIIRTVNVLGAHGVIIPKDRAASVTPAVIKASAGAVSFVPIARVVNLVMTI
                     RDLKKKGVWIVGADPSATKSVFEEELGTLDLALIVGSEGKGLTRLVREECDFLVSIPN
                     LGEVSCLNASVAAGIMIYEILRQRKTENSLII"
     gene            complement(3209..5284)
                     /gene="ligA"
                     /locus_tag="VI935_05265"
     CDS             complement(3209..5284)
                     /gene="ligA"
                     /locus_tag="VI935_05265"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HLE25042.1"
                     /translation="MKPGTKQIPKEDTKRRIKKLRREIERHNYLYYVESNPEISDAEY
                     DALVEELKNLEALFPELITPDSPTQRVSGYVAEGFNLVEHRVPMMSIDNISTEGDALE
                     FDKRVKRFLGIEVSQKPRHVGTLRDLTDGIGYTAEPKFDGVSASLTFENGLFIQGATR
                     GDGMVGEEVTTNLKTIKAIPLRLITSNGVPKRMEVRGEVILPIEAFRRLNKDLGEVGE
                     PIFANPRNAASGSLRQLDSSITAKRPLDFYAWGIGEVKGYEFKTQWEIVQTLRRWGFK
                     VEKKIMHCKNINEAISYYHEMESIRDDLPYEADGVVIKVDRLDYQRELGTTAKHPRWS
                     IAYKFKPRQATTKIRDIIVQVGRMGLLTPVANLHPVKIGGVTVSRSTLHTEELIRVKD
                     IRIGDTVLVERAGDVIPEIVKPIVENRTGEEKPFNMPKSCPSCGTKVDKDGAYYYCPN
                     LSCPAQLKGRIKHLASRRVFDIDGLGEKIVEQFMREGLIKDLADIFYLKKDDFIDLER
                     FAEKSASNLAEEIEKSKRTPFDRFIHALSIRHVGERLAQVLSQNFPNLDALIEASEER
                     LMEIPTVGPEVAKSIVNFFKERKNRKVIEKMLAAGVKIEYKFKKVGPIHELPLRAKTF
                     VFTGVLQSFTRDEAKRLVEELGGRVSSSVTKKTDYVVVGEDPGSKLDVAKSLEIKTID
                     EEEFKKLIA"
     gene            complement(5311..7035)
                     /gene="polX"
                     /locus_tag="VI935_05270"
     CDS             complement(5311..7035)
                     /gene="polX"
                     /locus_tag="VI935_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HLE25043.1"
                     /translation="MSKNREIGDIFDSIADILDLREDNPFRIRAYRNAARNIRELTEN
                     IENIAERDELTKIPGVGTDLSEKIKEFITTGKINQYDELKKKVPEGLVELLAIKGVGP
                     KTLAKLNKEIGVKNLNDLERALEGKEILTLRGMGQKKIEDIKRGIELLKQSGERMPLG
                     IALPLAEELIFEISKIPGTEGTIAAGSLRRMKETVGDIDILTMAENGERVIKAFTKLS
                     LVKEVLAAGDTKGSVILKNGMQVDLRVIEPGSYGAALQYFTGSKAHNIKLRTLALEKD
                     LKINEYGVFRGTENIAGETEEEIYKTLELPWIPPEIREDKGEIEAASDEKLPNLIELR
                     DIRGDLQMHSKWSDGKATIEEMALRARELGYEYIAMTDHSPSSRIAKGLSVERLYEKK
                     KEVEAINKKIKGMSILMGSEVDIKGDGSLDYPDTVLKHLDIVLVAVHSGFKMNSESMT
                     KRIVIALQNPYVHALVHPTGRLIGEREAYEVDMDEVIKTAKALGKALEVNSYYLRLDL
                     NDINVRKAIQTGVKIVISTDAHLPDQLSMMRLGVATARRGWAEKKDVVNTMDLKGLTK
                     WLSEVRGK"
     gene            complement(7182..8246)
                     /locus_tag="VI935_05275"
     CDS             complement(7182..8246)
                     /locus_tag="VI935_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25044.1"
                     /translation="MRIEKGMLINSIILFFLLFIPAYFIAYAETIEDESLTSEEIILT
                     EEADEGSENNRGSNIELATFPTPIFIEPNFIPSPFFFASPFFFPRFFVAPVFVNPFYL
                     SFGFSFFTNPFFISFGFPFFFNCPFFFSPFAFNSFIFFPHHHHHRYHYVHHIAHDKAS
                     KIVKTGVPINEPVINRVNKYTGSTQNVQNQLKAPGALKTIINPTVDNNSVKNQTDFAT
                     ERARKRILHGNNSTFKFWSKVDNNETGFRLFGGQDSTFPSNISGSNPMTLENRTQTYN
                     PMKNSGGRLRSYNRDNSSTKTNNNNGKPMSYNSPYIKSHRSQIGTSNFSSKFRSHSFD
                     RIGRTSVGKIGGSFRRFGSR"
     gene            complement(8369..10096)
                     /locus_tag="VI935_05280"
     CDS             complement(8369..10096)
                     /locus_tag="VI935_05280"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLE25045.1"
                     /translation="MSIRTPALTLLIALLLFPYSHLLADQGKDEILFQDSITLYKNLQ
                     GDPSKVSNYEIWYTLAQAFYSIYRTYPDSPNAPTSLFLSGKLYEQMGNRFNSRENLER
                     SIELNREFVRVFPDSNLSDDAQLRIARILEGWDKSGAYIEYEKVVREYPKGDMVEIAG
                     KKLDQFAAYKPKENKITDGGIAKAKTRDNSKGLARVNQIRHWSTDDSTRVVIHLDKEM
                     AFDSHLLKPDPKGGMPPRLYVDIKGTTVTRNLEMEPIEKGLLEDIKFARNTLDTVRVV
                     LYIKSFNNYKVFALYEPFRIVMDIYGDEISGEAIAKDREVEEEKSLSGLPKLDGKEIS
                     ALRGALGLKVRTVVIDPGHGGHDPGAIGPTGLKEKNVTLAIAKSLARKLEQDGNEFGI
                     TRVVLTREDDRFIPLEERTAIAKKERADLFVSIHCNAARNNNAHGIETYVLSFTNDPR
                     SLEVAARENVTTTRGISDLKDIIKKYLLNSKIDESKRLASHVQSSVTTNVARKYSTVK
                     SIGVKKAPFIVLIGADVPSILVETSFITNPREEKRLKNPNYLDEIADGIFAGVKRYSN
                     EVETASVAQ"
     gene            10247..11014
                     /locus_tag="VI935_05285"
     CDS             10247..11014
                     /locus_tag="VI935_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLE25046.1"
                     /translation="MRVIILGCGTSTGVPIVGCNCSTCTSNNPKNKRTRTSVVVQTHK
                     KNILIDTSTDLRFQALTHDITRIDAVLYTHSHADHTHGIDELRTYNFINKMVIPCYGN
                     PQTVSTIKMNFRYIFDGSYAAAGKPQLEITSVTDEFDFHGIRITPIEINHADWLIFGY
                     RIGKMAYLTDCSGIPEKSVKKLRGLDLLIIDALRYTPHSAHFSVDQAVERTRSIEPRI
                     AVLTHMGHELEYDELSRQLPEGIMPAYDGMEFGLDEA"
     gene            11106..12329
                     /locus_tag="VI935_05290"
     CDS             11106..12329
                     /locus_tag="VI935_05290"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HLE25047.1"
                     /translation="MQEIIRDLLIPNDSKIVLCVLDGLGGLPLDGKTELEAASTPNLD
                     ELARRGACGLHIPVARGITPGSGPSHLALFGYNPLKYEIGRGVLEAIGLGIELSPSEV
                     AIRGNFATVRYEGNKPIVVDRRGGRILTEENKRIAERIRERIQSLDGIRVSITSGMEH
                     RFALVLTFPEPLPPGADDVTDTDPQKEGKAPAKPSPRRKEAERVARVAERFIELAAEI
                     IKDEKVANYLLLRGFSLYPNLPQFSQTYGLKAATTAAYPMYRGVAKLVGMEVLEVSDT
                     STKSEVETLKRYFNEYDFFYFHVKKTDSYGEDGNYWGKVKVIEEFDSIVPEISNLKPD
                     VLVVTGDHSTPAVLKSHSWHPVPFLLHSPYTRGGGSLGFSEKECLKGELGIFRATDAM
                     TLILSHARRLQKFGA"
     gene            12558..12644
                     /locus_tag="VI935_05295"
     tRNA            12558..12644
                     /locus_tag="VI935_05295"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12592..12594,aa:Leu,seq:gag)
     gene            complement(12942..13091)
                     /locus_tag="VI935_05300"
     CDS             complement(12942..13091)
                     /locus_tag="VI935_05300"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HLE25048.1"
                     /translation="MRRWCVIMKEIEDLIEKAKRFLRSAEILFHDGDYDSCVSRCYYA
                     MFFMT"
     gene            complement(13312..13638)
                     /locus_tag="VI935_05305"
     CDS             complement(13312..13638)
                     /locus_tag="VI935_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLE25049.1"
                     /translation="MAKPGNIFSNIPKHIPQEIFEEIIATENIKIERIISKGHATPDD
                     KWYDQDKNEWVILLKGSAGLLFEGEDNPITLDPGDYILIPAHMKHRVEWTDQTEMTIW
                     LAVHYD"
     gene            complement(13760..14914)
                     /locus_tag="VI935_05310"
     CDS             complement(13760..14914)
                     /locus_tag="VI935_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25050.1"
                     /translation="MNRFIISLCFATLLFVLPAIATQDEEGSKDLKLVTVPSGEVINK
                     DYFAFGDKIEISGTVNGDVYAAGGQILIDGTINGDLLAAGGKISISGNISQDVRIVGG
                     QITIGGEIGRSLTVSCGNVELTDSAIVHGSVVAGGESILLASPIGREVMIAAGNLTVS
                     NKVDGNLEAFVGSIRLTSKAVINGDLIYWSDRPASIDRNAKITGSVTQKTPPMPSPEK
                     AFGIFARICLLATLISFISTLIIGLLLIYFYPKYNRGVVSTLRKNPLTSLGIGFLTLV
                     VAPIFLIIIFSTVVGIPLALILLASYLITIYLARIYVIFWAGVTIFERLGKRVHEGWA
                     LVVGLIIYFILTIIPVIGGIITFVVVLFGLGAAILTKKELYQTLRKQDVI"
     gene            15247..16017
                     /locus_tag="VI935_05315"
     CDS             15247..16017
                     /locus_tag="VI935_05315"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HLE25051.1"
                     /translation="MQKKTRLIDLSMKIEPAPGPEHQLLQIKHEAHNETAEYMMKRFQ
                     CRRGDLPKGLGWANDYVTLGTHVGTHIDAPWHYHPTSEGNKAKTIDEVPVEWFYGDGV
                     VIDMRHKKPGELITVSDLEEALDKISYKIKPFDIVLVMTGADKLWGTMDYWSKFPGLG
                     RESTLWLCNQGVKVMGTDSAGWDRPFAAMVEEFKRTGDSSVLWGAHFAGIEKEYCQIE
                     KLTNLGLLPPYGFKVACFPIKVLGGSAGWVRPVAIIEE"
     gene            16105..16563
                     /locus_tag="VI935_05320"
     CDS             16105..16563
                     /locus_tag="VI935_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HLE25052.1"
                     /translation="MMNVQKVNDELNKALSLEYASVIQGLQHSFLVQGLFRESIAPML
                     RDHAEESFKHAKILGDKIVALGGLPTVEPAPVKQASDLVEMLKQDLALAREVYEHYES
                     LLKMVQDDTPLRIMVENFVETEKKDCEELEKLLEIRKIRVKDKEVTFRVK"
     gene            complement(16676..16963)
                     /locus_tag="VI935_05325"
     CDS             complement(16676..16963)
                     /locus_tag="VI935_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25053.1"
                     /translation="MISQATEALLGSIYGEDNDQIIEVIVKEEENRFEIRLLSYGEGL
                     GWYVQKTLPIDSSQAEALVKILSKTNNAAEKKLIRHDDVSTFGKVIRFPLA"
     gene            complement(17325..17753)
                     /locus_tag="VI935_05330"
     CDS             complement(17325..17753)
                     /locus_tag="VI935_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25054.1"
                     /translation="MKLILLKALILLSLFYSIGKANDAVIKQSMIQHPEKFINITDWS
                     FFVPFGKVAIIHHVTIENNSDIAYKDIKVRVSYYSGSYSNYGTKIGGETGILPITLPP
                     HSKKTYLEGGSVLGSGSSLMYAGNIEVLGAIPLTEGGVSN"
     gene            18163..19353
                     /locus_tag="VI935_05335"
     CDS             18163..19353
                     /locus_tag="VI935_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HLE25055.1"
                     /translation="MAKRIILYTGKGGVGKTTVAAATALLAAKKGYKVLVISTDAAHS
                     LGDSFDMELGPEPKEIMPNLFAQEVDVYYSVEKYWGKLTEYLQSLLRWLKVDEILTEE
                     FSILPGMEEVSSFLWVYHHFQDELYDVIIVDSAPTGETLRLLSLPDVARWWIVKVFPI
                     ERKVVKVIRPAMKVVTDMPLPEDHTYDAIEELFDKLDSIHNTFSNPEITSIRLVLNLE
                     KMVIKETQRAYTYLNLYGYPVDSVIINRTMPKELEHPFFEEWKKSQLGYREEVEELFS
                     PIPTFEAKLFSKEVLGIESLEEFGSNLFSKKDPIRIFFKGKPYEIIKEGGSYQLLLKL
                     PFVSKEEIKLFQIKDELTIQVENHRRNILLPRFLADLPVKEAKYQDEMLRIIFEKKER
                     KPRG"
     gene            19754..20017
                     /locus_tag="VI935_05340"
     CDS             19754..20017
                     /locus_tag="VI935_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25056.1"
                     /translation="MIKALIAFMLVVTLITGMYSCSAITGNSQSVAEEEEEPPGAEYD
                     PVLRRSEPFTGKGAPLYDPLKEEEIESWENPLQTEEMESKPLF"
     gene            20345..20797
                     /locus_tag="VI935_05345"
     CDS             20345..20797
                     /locus_tag="VI935_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLE25057.1"
                     /translation="MLNKTIRSLILGVLIIGILQIYNSVSAESNPPNINSETQVIKDI
                     TPDEAFLLIEKNKDNSNLVILDVRTSKEFEDGHIKNAINLDFYSETFKDDLDKLDKNK
                     IYVTHCQLGGRSAKTVVMMKDLGFVEVYNITGGIAEWKSKGLPTIKSP"
     gene            complement(21424..21840)
                     /locus_tag="VI935_05350"
     CDS             complement(21424..21840)
                     /locus_tag="VI935_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25058.1"
                     /translation="MSEKTAKAIQLLNKLADVEAGEIDVDSRHIAATKNWLVRMFYDY
                     GLEDAKRRLEISTMIRSWLEQGKPLVGKIDVSATDLEREADSESSVGSLVKELLDLVD
                     DADAKALLQGILSDEEKHEQGLRSHTRNAGTKEKRC"
BASE COUNT         6373 a         4558 c         4646 g         6860 t
ORIGIN      
        1 caagtaagag gagaaaacca gaggcaaggg ctgctatagg tgggttgaga taattggtta
       61 agtatagata aagggcccag acgataaacc caagcccaat caccatgaaa gcccatgccg
      121 ctcctaaaag taaaaaactt aatcctagac gcaacaagac catgcgtagc agcttggatt
      181 cattaactag aagttgaata agcgtaacta taagcttaaa catagtctcc agaatctatc
      241 tacgattaag aagattgccc agtaaaaatc ctactaaaaa ggctaagact agactcaaga
      301 gaggtttttc cttaacctct ttttccattg tcttaaatgt ctttttgcct ctttcgctga
      361 cctgatctaa ctcctcaacc agttcatcta tgaggtttcg tgcttcggtt tctaacttct
      421 ccttagcctc tttggcctca gtctttccta tgtcaatcag cttcttggct atttcagtca
      481 tatccgacct cagcttaacc atatcctcct ttaaggttac tatatcttcc tgaaacttac
      541 gctctgtcat cggccacctc ctaaagttga tcttgcacta gcaagcctgt cccgagtaaa
      601 tcgaggggac cggtgggcta cccgattatc cccccctcac cctgaatact ccccgcaagg
      661 agagaagaac ttttgccggt ggacaaccct tccctgcaac ccacgctctt ccatcggcta
      721 gctcctaaaa gtttactgcg ttactcatat attgccctat taggataaac tgtataataa
      781 tatttattca attaatttta aaggggatat ttaatgagaa ggcaaaatag atttagtagc
      841 ataggaaaga catttttata tttgatattt cttgcactag taataggtgg tttcatattc
      901 atttttcctc gatttgaatg gcatgcgcca accgtagaaa tgaagcttga ctctgagttt
      961 atagggcttc gtccctttga tgttgtgata aaagacaaag gtaaaggatt aaaaagggtt
     1021 tctattggac taacaaatga acaaggagag tttccactca ttcaagaata ttactcgcta
     1081 ccagtgatgg agaagaactt cgctatcgca cttgacccga agaagctcgg cataaaggat
     1141 ggccctgcta caatacgagt gacggctgtt gatggttctt attggggatt ttttaagggt
     1201 aataagacaa cattaagtaa agatgtcaag attgatgtta cccctcctag ggttgaagtg
     1261 cttagtagag agaactacat taatttcggt ggctcagggg ctgtgattta taagacctct
     1321 aaagattctg ttaagaacgg tgttaagatc ggtgattatt tctttaaggg atataaaggc
     1381 tacttcaata accctgatgt ttatatcgct atatttgctt atccttacaa tgtacctacg
     1441 gataaaaaaa tagtggttat tgctgaggat ggcgctggaa attcaaaaga ggtaggattc
     1501 tcctacagac taaaggacgt taaatataga aaaagctccc tcgatataag tgatgatttc
     1561 attgaaagaa aagtggttcc actcttgggg gatgattcaa atcaaggtga tgatcttaag
     1621 ggatctttcc tcaaagtaaa ccgggagcta agaaagaaaa acgaggatga gattaaacgg
     1681 attggtgaaa aatcatcgag ttctatacta tggagcgggg gctttcatca gcttacaaac
     1741 tcgaaggtag aggctaattt tgcagatgca agaacctata tttacaacga agaggtaata
     1801 gataatcagt accatctggg ttatgacttg gctgtaacac aaaaatatcc tattgaagca
     1861 gcaaacgatg gtgttgtttt atttgcaggg gatttaggga tatatggaaa tactgtcata
     1921 attgatcatg gactaggtgt cgctacactt tatggtcaca tgagttcaat tgatgtaaag
     1981 gttggagata aaatcaagaa aaaacagata atcggaagaa ctggtgatac tggtcttgca
     2041 gcgggggacc atctacatta cgcggtgctc ctagacgggg ttccagttct accaattgag
     2101 tggtgggatg aaaagtggat taatgacaat atcctgaaca aaataaaaga agcagagttg
     2161 aattttggca ctcggagtac tcagggtgat atttctgaag agagtccttc acaacctaag
     2221 gtacagtaag attgatcatt tacggaaaaa accctgtaaa agaagctatt tcccaaacat
     2281 tgtctaagat agaagaaatc ttaatttctc aggagttaga gaaagaaagg atttcagata
     2341 ttataagact cgcaagagag aagaatgtaa aggtttcatt tcttccacgt gatgcaatct
     2401 caagaatcgc agggacttct catcatcaag gcattgcagc tagggtttca gagtttgagt
     2461 acaataatgt tgataatatt cttggcaacg caaagcaaaa gggagaaaga ctccttatca
     2521 ttttacttga tcacattgaa gaccctcaga atctaggggc aataataagg acggttaatg
     2581 ttcttggagc acacggtgta ataatcccta aagatagggc cgcctctgtg acacctgctg
     2641 tgataaaagc atctgcaggt gcggtgagtt ttgttccgat tgcgagggtg gtaaatcttg
     2701 ttatgacaat aagggattta aagaaaaaag gagtatggat tgtaggagct gacccttctg
     2761 ctactaaatc tgtttttgaa gaagaacttg gcactttgga tttagccctt attgtgggaa
     2821 gtgaagggaa gggtttgacg agattagtaa gagaagaatg cgattttctt gtatctatac
     2881 ccaatctagg agaggtttca tgtcttaatg cctctgttgc agcagggata atgatttatg
     2941 agatattgag gcagaggaag actgagaata gtttaataat ataattttga aagatgccag
     3001 aatggtgttg tcacattttt gaattgcatt aagaccatat tattgaaatg ttgcgtaaca
     3061 tacttaagct ggctccgcca gtaagactgg cggactaccc tagttgtatt tgttgggtag
     3121 tcaatgagtc ttgctcgttg gcttaacaca cgagtataat gcgatcctta ttcaagagag
     3181 ttttattaca aacttagtgg gtgtatctct acgcaataag ttttttgaat tcttcctcat
     3241 caatggtctt aatttctaag gatttagcaa catcgagctt cgagcctgga tcttctccaa
     3301 ctaccacata atcagttttc ttggtcactg aactggaaac tcgacctccg agttcttcaa
     3361 caagtctttt tgcctcatct ctggtaaaag actgaagaac tcctgtaaaa acaaatgtct
     3421 ttgcccgtag tggcaattca tgaattggcc ctaccttttt aaacttatac tcgatcttta
     3481 cgcctgcagc cagcatcttc tcgattactt ttctattctt tctctcttta aaaaagttaa
     3541 cgatgctctt tgctacttcg ggaccaaccg ttggaatttc cattagtctt tcttctgaag
     3601 cctctataag ggcatcaaga ttggggaagt tttgtgaaag tacttgagca agcctttcac
     3661 cgacatgacg tatacttaaa gcatgaatga agcgatcaaa tggggttctt ttactcttct
     3721 caatctcttc tgcaagattt gaagcagatt tctcagcgaa tctctcaaga tcaataaaat
     3781 catccttttt taagtagaag atatcagcta aatccttgat taaaccttct ctcataaact
     3841 gctcaactat cttttcgcca aggccgtcaa tgtcaaatac ccttctggat gcaaggtgtt
     3901 ttatcctgcc ttttaactgt gcaggacacg aaaggttagg gcagtagtag taagccccgt
     3961 ctttatccac cttcgttccg catgagggac atgacttagg catattgaag ggcttttctt
     4021 caccagttct gttctcgacg ataggcttta ctatctctgg tataacgtcc cccgctcttt
     4081 ctacaagtac cgtgtctccg attcttatat cctttactct gatcaattcc tctgtatgta
     4141 gggtagaacg gcttacagtc acgcctccga ttttgactgg atgtaagttt gcaacaggtg
     4201 taagtaaccc cattcttcca acttgaacga ttatgtctcg gattttagtt gtggcctgac
     4261 gtggtttaaa cttgtatgcg atactccatc gtgggtgttt agctgttgtg ccaagctccc
     4321 tctggtagtc taatctatct acttttatca caacgccgtc ggcttcatag ggcaagtcat
     4381 ctcttataga ctccatttca tgatagtagg aaattgcttc atttatattt ttacagtgca
     4441 tgatcttctt ctctacctta aatccccacc gtctaagcgt ttgaacaatc tcccattgag
     4501 ttttaaattc atacccttta acctcaccaa ttccccaagc atagaaatca agaggtcttt
     4561 tagcagtgat gctcgaatca agctgtcgga gcgagcccga ggcagcgttt cttgggtttg
     4621 caaaaattgg ctctcctact tcgccaaggt ctttgtttaa ccttctaaaa gcctctattg
     4681 gcaatatcac ctctcctctc acctccattc tcttcggaac cccgtttgat gtgataagcc
     4741 taagcggaat tgcttttatg gtctttaaat tagtagtaac ctcttcccca accatgccgt
     4801 ctccgcgagt tgcgccttga ataaataatc cattctcgaa agtaagagac gcagaaacgc
     4861 cgtcgaattt tggttctgct gtatatccaa taccatctgt caggtctctc aaagtaccca
     4921 cgtggcgggg cttttgcgaa acctctattc caaggaacct ctttacacgc ttatcgaact
     4981 caagcgcatc accctccgtt gagatgttat ctatgctcat cattggaaca cggtgttcga
     5041 caagattaaa tccttctgca acatagccgc ttacacgttg tgtgggggaa tctggggtta
     5101 tcagttctgg aaatagagcc tcaagatttt ttaactcttc tactagcgca tcatattcag
     5161 catctgaaat ctcaggattg ctttctacat aatagagata gttatgacgt tctatctcac
     5221 gtctaagttt tttaatcctt ctttttgtgt cttccttagg gatttgcttt gtgcctggtt
     5281 tcatctaatc gatttaacca ccttttactt ttattttcct cgaacttcgc ttagccactt
     5341 agtaagccct ttcaaatcca tagtatttac aacatccttt ttttctgccc aaccccttct
     5401 tgctgtagca acgccaagcc tcatcatgct gagttggtct ggaagatggg catcggtgga
     5461 gattactatc ttaacacccg tctggattgc cttccttaca ttaatatcat tcagatcaag
     5521 tcttaagtaa taggaattaa cctcaagagc ctttcctaat gctttagctg tcttaataac
     5581 ctcatccatg tcaacctcat atgcctcacg ttccccgatt aaccttcctg tagggtgaac
     5641 aagggcatgg acatagggat tttgtagagc tattactatc ctttttgtca tggattcact
     5701 gttcattttg aatccactgt gcactgctac aagcacaata tctaagtgtt ttagaaccgt
     5761 atcaggataa tcaagtgaac cgtcgccttt tatatctacc tctgaaccca tcaggatgct
     5821 cattcctttt atcttcttat ttattgcctc aacctctttt ttcttttcat agagtctttc
     5881 aactgaaagc ccttttgcaa tcctcgaaga aggagagtgg tcagtcatag cgatatattc
     5941 ataacctaac tctcttgcac ggagagccat ttcctcgatt gttgctttac catcactcca
     6001 tttagagtgc atttgtagat cacccctaat atccctaagc tcgataagat ttggtagttt
     6061 ttcatccgat gcggcttcaa tctcaccctt atcctctcta atctctggag gaatccaggg
     6121 aagctctagg gttttataaa tctcctcttc tgtttctccg gctatattct ctgtccctcg
     6181 gaagactccg tactcgttta ttttgaggtc cttttctagg gcaagtgttc taagttttat
     6241 gttatgtgct ttcgatccgg tgaaatactg aagagctgcc ccatatgatc ctggctctat
     6301 tactcgaaga tctacctgca tcccgttttt tagaattacg cttcctttgg tatcccctgc
     6361 tgctaaaacc tctttgacaa gagaaagctt tgtaaaagcc tttataacac gctccccatt
     6421 ctctgccatt gtaagaatat caatatctcc tacggtttcc ttcatacgcc tgagtgatcc
     6481 tgctgctatg gttccctctg tgcctgggat tttacttatc tcgaagatta attcttcagc
     6541 tagcgggagg gcaattccga gaggcatcct ttccccactc tgttttaaaa gctctatccc
     6601 acgttttata tcctcaatct ttttctgtcc catacctcta agtgttaaaa tttcctttcc
     6661 ctcaagtgct ctttcaagat cattcaagtt tttgacacca atttctttat ttagtttagc
     6721 cagggtcttt ggacctactc ccttaatagc taatagttcg acgagccctt ctgggacctt
     6781 cttttttaat tcgtcatact gatttatttt tcctgtggtg atgaattcct taatcttctc
     6841 tgagaggtcg gttcctactc caggtatttt agtaagctca tctcgctctg caatattctc
     6901 gatattttca gtgagttctc ttatatttct ggctgcgttt ctgtatgctc tgattctaaa
     6961 agggttatct tcacgaagat ctagtatgtc tgcaatgcta tcgaaaatat cgccgatctc
     7021 tctattcttg ctcataatat tctaagtata ttgtaggtaa aaagggtttt aaagtagaga
     7081 cgacccagcg gctcgtctct ataaaatagg attatctctt ttcaaatcgg ttaaatgaac
     7141 tgatcattat atggcgatct aaaggtcgcc gttactacag gttatctact tccgaatcgg
     7201 cggaatgaac cacctatctt accgactgat gtccttccga ttcggtcgaa tgaatggctt
     7261 ctaaatttgc ttgagaaatt tgatgtacct atttggcttc ggtgactctt tatgtaagga
     7321 gaattgtatg acatgggttt gccattatta ttgtttgttt tagtactgct gttgtccctg
     7381 ttatagcttc taagcctacc accagagttt ttcataggat tataggtttg cgttctattc
     7441 tctaaggtca ttgggtttga accactgata tttgaaggaa acgtactatc ttgacctcca
     7501 aataatctga agcctgtttc attattatca acttttgacc aaaatttgaa agtggaatta
     7561 ttgccatgca aaatcctttt tctagcgcgc tctgtggcaa aatcggtttg gtttttaaca
     7621 gaattattat ctactgttgg atttataatg gtctttaaag cgcctggcgc ttttagttga
     7681 ttttgtacat tctgcgttga acctgtgtac ttatttacac gatttattac aggttcatta
     7741 ataggtacgc ctgttttgac tatttttgat gccttatcat gcgctatatg atgaacataa
     7801 tgataacgat ggtggtgatg gtgtgggaag aatataaatg aattgaaggc gaatggtgag
     7861 aaaaagaagg ggcaattaaa gaagaatgga aaaccaaaag agataaagaa cggatttgtg
     7921 aaaaaggaga atccaaagga aagataaaaa gggttaacga agacaggagc aacaaaaaac
     7981 ctagggaaga agaacggact tgcgaagaaa aacggagagg gaatgaaatt tggctctatg
     8041 aaaataggag tggggaaggt tgcaagttca atattggatc cacgattgtt ctcacttccc
     8101 tcatctgctt cttcggtaag gataatttct tcacttgtaa gagattcatc ttcaattgtt
     8161 tctgcatatg ctataaagta agctggtata aatagaagaa aaaagagaat aattgagtta
     8221 attaacattc ccttttctat cctcataagg catccctccc caatttatgt aactttaatt
     8281 atattctacc acaggttgaa ttaaagatgg acacaattaa atggtgggtt ggaacaatga
     8341 tataaggatt attgctatag ggaaggggtc actgtgctac agatgctgtt tctacctcat
     8401 ttgagtaacg ttttacgcca gcaaatatac catctgcaat ttcgtctaga taatttggat
     8461 tctttagtct tttttcttct cttgggtttg taatgaatga tgtttcgacg agtatgcttg
     8521 gaacgtcagc gccaattaat actataaagg gcgctttctt tacccctata ctttttaccg
     8581 ttgaatattt tcttgctacg ttagttgtaa ctgaactctg aacgtgggaa gcaagccttt
     8641 tagattcatc aatcttggaa ttgagtaggt atttttttat aatatctttt agatcactaa
     8701 tgcctcttgt cgtggtaacg ttctcacggg ctgcaacttc gagtgaacga gggtcatttg
     8761 tgaagctgag tacgtaggtt tctataccgt gggcattatt attcctagcg gcattgcagt
     8821 gaattgatac aaaaagatct gccctttcct ttttcgctat agcggtcctt tcctctagcg
     8881 gtatgaatcg gtcatcttct cttgtaagaa cgacgcgtgt gattccgaat tcgtttccat
     8941 cttgctctag ttttctcgct agggattttg caatagcaag tgttacattc ttttccttga
     9001 gccctgttgg accgattgcc cctgggtcgt gacctccgtg tccggggtct atcacaacgg
     9061 ttcttacttt taatccaagc gctcctcgca gggctgaaat ctcttttcca tctagttttg
     9121 ggagaccaga gagactcttt tcctcttcga cttctctatc tttcgcaatg gcttctccac
     9181 ttatctcatc gccgtagatg tccattacga ttctgaaagg ttcgtataat gcgaatacct
     9241 tgtagttgtt gaagcttttt atgtaaagta ctacccttac ggtgtcgagt gtatttcttg
     9301 caaattttat gtcctcaagc aagccttttt cgattggctc catctcaaga ttccgggtaa
     9361 cagtggtgcc ctttatatca acgtaaagcc taggtggcat gccacccttt gggtcgggtt
     9421 tgaggaggtg ggagtcgaat gccatttctt tatcaagatg tattacaact cttgtagaat
     9481 catccgtaga ccagtggcgt atttggttta cgcgggctaa tcctttcgaa ttatctcgag
     9541 ttttagcctt cgcaatacct ccatctgtaa ttttattttc ttttggttta taggctgcaa
     9601 actgatcaag ctttttccca gctatctcaa ccatatcacc ttttggatat tcccttacaa
     9661 ccttctcata ttctatatag gcccctgact tatcccaacc ttcgaggatt cttgctatcc
     9721 taagctgtgc atcgtcggaa aggttgctat cgggaaaaac ccgcacaaat tccctattga
     9781 gttcgataga cctctcaaga ttttcccttg aattaaacct gttgcccatt tgttcataaa
     9841 gctttcctga taggaataac gaagttggag cattagggct atcaggatag gttctataaa
     9901 tgctgtagaa cgcctgtgca agtgtatacc aaatttcata gttgcttact ttcgaaggat
     9961 ctccttgtag gtttttataa agagttattg aatcttgaaa aaggatctcg tcctttccct
    10021 ggtcggccaa taggtgggag tagggaaata gaagtaaagc tataagtagc gttagggcgg
    10081 gggtcctaat gctcaattta atcacataat aaatgttttt tagggctttg taaaccactt
    10141 tttaaaaaat tgtttaatag gtattccgag gcgcccttag ctttcataat ttaacaatta
    10201 gataagggtt tttgaaatga gggcgactag gtgctaaact ttgcaaatga gggttataat
    10261 acttggatgt ggtacgtcta ccggtgttcc tatagttggc tgtaactgtt ctacttgtac
    10321 ttcaaataac ccgaaaaata aaagaacgag gacctcggtt gttgttcaaa ctcataaaaa
    10381 gaatatcctg atagatacct ccactgacct caggtttcaa gccctaactc acgacattac
    10441 aagaatagat gctgtccttt atacccattc acatgctgac catacacacg gcattgacga
    10501 gcttaggaca tataacttta ttaataaaat ggtaatccca tgctatggaa atccccaaac
    10561 ggtttcaaca atcaaaatga actttaggta tatattcgat ggctcatatg cggcggcggg
    10621 taagccgcaa ctggaaataa catctgtcac ggatgaattc gattttcatg gaataaggat
    10681 taccccgata gaaatcaatc atgcagattg gctaatattt ggttacagaa taggaaagat
    10741 ggcttatctt acagactgca gtggaattcc tgaaaagtca gtgaagaagc tgagagggct
    10801 tgacctgctc ataatagatg ctttgagata tacacctcac tcagcccatt ttagcgtgga
    10861 tcaagctgtt gaacgtactc gtagtattga gcctagaatc gcagttctta cacacatggg
    10921 gcacgagctt gaatacgatg agctttcaag acagcttcca gaaggtatca tgcccgctta
    10981 cgatgggatg gaatttgggc ttgatgaagc atagatgtag gggctgcata tatgcagccc
    11041 tggcgtggga tgcactgtgt gcattcccta cattaaaatc aaataccaaa taggagcata
    11101 cttaaatgca ggaaataatt cgcgatctgt taattccgaa tgattcgaag atagttctat
    11161 gtgtattaga tgggcttgga gggcttcccc ttgatgggaa aacggagctt gaagcggcct
    11221 ctaccccaaa cctggatgag cttgcaaggc gtggcgcctg tggacttcat atccctgttg
    11281 cacgtggtat tacaccagga agcgggccct ctcatctggc attatttggc tataatcccc
    11341 ttaaatatga gatcggccgc ggtgttttag aggcaatcgg gctaggaatc gagctatctc
    11401 ccagtgaggt ggcaattcgt ggaaacttcg caactgtaag atatgaagga aataaaccaa
    11461 ttgttgtaga tagaagggga gggcggatac tgacagaaga gaataaaaga atcgctgaga
    11521 ggattcgaga aaggattcaa agcctagacg gcattagggt ttccatcacc tctgggatgg
    11581 agcacagatt tgcattagtg cttacatttc ctgagcctct tccccctggt gcagatgacg
    11641 ttaccgatac agatcctcag aaggagggaa aagctccagc taagccaagt ccgagaagga
    11701 aagaggccga gagggtggct cgtgtagccg agagatttat tgaactcgca gcagagatca
    11761 ttaaagatga gaaggtggct aattaccttc ttctccgagg cttttcactt tatccaaatc
    11821 taccacagtt ttctcagacg tatggactaa aggctgctac aacggctgca tatcctatgt
    11881 atcggggtgt cgcaaagctt gttggaatgg aggttttaga agtgtcagat acctctacta
    11941 agagcgaggt tgaaacacta aagagatatt ttaatgaata tgatttcttc tacttccatg
    12001 tgaaaaagac agacagctat ggtgaggatg gaaactactg gggaaaggtt aaggtgatag
    12061 aagagtttga tagcattgtc cctgaaattt ctaatcttaa acctgatgtt ttggttgtaa
    12121 caggcgacca ttctacgccc gctgttttga aatcacatag ctggcatccc gtaccttttc
    12181 ttcttcattc tccctacact cgtggtggag ggagtttggg attctctgag aaggaatgcc
    12241 ttaagggaga gctaggcatt tttagggcaa cagatgcaat gacccttatt ctttctcacg
    12301 caagaaggct tcagaaattt ggcgcgtagc atttggaggc agtgttagaa aacaccgcct
    12361 atcgcgtcgt ttgaatcaaa gaactatagg cgggacattc ctgtcccgca ttggctcagt
    12421 tcgaaggaac tccttgaatg cacagataga aaagggtagt ttccgccagt cttgctggcg
    12481 ggattatatg cagagcccac tgaacttgcc tctttaacaa cagtatgcta atttaatttc
    12541 tcgttaaaaa gaaacgtgcc gaagtggcgg aactgggaga cgcgctgtct tgagggggca
    12601 gtgccagtaa tggcgtgcgg gttcgactcc cgccttcggc accactggaa agagtcttta
    12661 tctatttttg ttaagaagta agcaaccttt taagatacct agtttttaag atagttttcc
    12721 acctcagtca caatactttt agctctttct aagtttttct tagcctcttt ctcagcaaca
    12781 acaaagcctg tagagtaatc cccttctgcc ttaaatcgta tgccctagtt agtgctttac
    12841 caatttcctc cttgaatata ttcgttttga taaaatgctc tccgaataag cgtataccct
    12901 tattagacga tgctctaacc ccccttaaag aacagcacag cttaagtcat aaagaacatg
    12961 gcatagtaac atcttgagac acatgaatcg tagtctccgt catgaaataa tatctctgca
    13021 ctcctcagga atctttttgc cttttctatt aggtcttcta tctctttcat aatgacacac
    13081 catcttctca ttttcccctt tctattattt taacccaaga atatgatttt atcttttcta
    13141 gaacaaaata gtgtgaaatc gaaagaatgt agtaagggta acatatataa cctctttctt
    13201 cggctagagt gaaggtgttt gacttctcgt ctgacgaccg tgctgagggg tgaaggtttg
    13261 acacgtaatg cactgtgtat gaggttacac caagagtgtt taaggttttt atcagtcgta
    13321 atgtacagct aaccagatcg tcatttcagt ttggtcagtc cattctactc gatgtttcat
    13381 gtgtgctgga atgaggatat aatctccagg gtccaatgtg atagggttgt cctcaccttc
    13441 aaataatagt cctgcgcttc ctttgagtag aatgacccat tcatttttat cttgatcata
    13501 ccacttatcg tctggggttg cgtgtccttt agagatgata cgctctattt tgatgttttc
    13561 tgtagctata atttcttcga atatttcctg tggaatatgt ttcgggatgt tagaaaaaat
    13621 gtttcctggt ttcgccatat tgcgtcttac cttttcctat aatttctcta attttgcaag
    13681 ggcgtttgag aaaatccaat agtaaaaaat atacaagaaa ttgaccgtta ttaactatgt
    13741 ctttattgca agttgggagt taaataacat cctgctttct cagggtttgg taaagctctt
    13801 ttttagtcag aattgctgct ccgagaccaa aaagtacaac tacgaaggtt attatgccac
    13861 caattactgg tattattgtt agtatgaaat agatgattaa tccaacaacc aaagcccagc
    13921 cttcgtgaac cctctttcct aacctctcaa atatggttac gcccgcccag aagattacat
    13981 agatccttgc caagtatatt gtaattaaat atgaagctaa aagtattagc gctaatggaa
    14041 tgccaacaac tgtactaaaa ataattatca aaaatatcgg tgccacaact agagtaagaa
    14101 atccaattcc taacgaagtt aagggatttt ttcttaaagt ggaaacaacc cctcgattgt
    14161 atttgggata gaaataaatt aataaaagcc cgatgattag agtagaaata aagcttataa
    14221 gtgtcgcaag aagacaaatt ctggcaaaaa ttccaaatgc cttttcaggc gatggcattg
    14281 gaggtgtctt ttgggttaca cttccagtaa tttttgcatt tctgtcaatt gaggcaggtc
    14341 tatcactcca ataaatgagg tctccattaa ttactgcctt tgaggttaaa cgaattgatc
    14401 cgacaaatgc ttccaagttc ccgtctactt tatttgaaac tgttaggtta ccagcggcga
    14461 tcattacctc tctaccaatt ggagaggcta aaagaatgct ctctcctccg gctacaacgc
    14521 taccatgaac tattgccgaa tcagtaagct caacgttgcc gcaactaact gttaggcttc
    14581 taccgatttc accaccgatt gttatttgtc caccaactat tcgaacgtct tgagaaatat
    14641 ttcccgaaat actaatcttg ccacccgctg ccaagaggtc cccatttatc gttccatcaa
    14701 tcaaaatctg cccgccagca gcatagacat caccattgac tgttcctgaa atttctatct
    14761 tgtcaccaaa tgcaaaatag tctttattga taacctctcc tgagggtacg gtaacaagct
    14821 ttaaatcttt tgaaccctcc tcatcttgtg ttgcaatagc tggtaaaaca aaaagtaaag
    14881 ttgcgaagca caacgaaatt ataaacctgt tcatatcggg cagtttaaaa ctccattata
    14941 tttctgtcaa gtgagttact tgtaaattcg caatgggtat tgtattgaca aagtacgcag
    15001 aattttaaat tacgataggc agggttaacc ccgtatgtaa tcctccacgc taaccctcac
    15061 cagtctatac agggaggatg ggagtgggtg aattatgatg cgagacaaaa agtctcctat
    15121 cattcttcta tctcaaacaa ttcgataggc ggtgttttcc aacactgccc ttgagagcgg
    15181 gacaggaatg tcacgactat cgcgggatgc tattgataaa ccatgattcg gatataaatt
    15241 aaacctatgc agaaaaagac aaggctcatt gatttaagca tgaagattga acccgcaccg
    15301 ggtcctgaac accaacttct tcaaataaaa catgaagcgc acaatgagac tgcagagtat
    15361 atgatgaagc gctttcagtg tagaagaggg gatcttccaa aaggtctagg ttgggctaat
    15421 gactacgtta cgctaggcac tcatgtagga actcacattg atgcgccctg gcactatcac
    15481 cctacctctg agggtaacaa ggcaaaaaca atagatgagg ttcctgttga gtggttttac
    15541 ggggatgggg ttgtaataga tatgaggcat aagaaaccag gggaactaat caccgtatct
    15601 gatttagaag aagcactgga taagatctca tacaagatta agccatttga tattgttcta
    15661 gttatgacag gagcagacaa actctggggg acgatggatt actggtctaa atttccaggt
    15721 ttaggaagag aatcaactct ctggctttgt aatcagggcg ttaaggtgat gggaacagac
    15781 tctgcaggat gggatagacc atttgcagca atggtagagg agttcaaaag aactggtgat
    15841 agcagcgttc tctggggtgc acactttgct gggatagaaa aggagtattg ccagatcgaa
    15901 aaactaacca acttaggcct ccttccgcct tacggattca aggtagcttg ttttcctatc
    15961 aaggttcttg gcggaagcgc aggctgggtt aggccagttg ctattattga agaatagaaa
    16021 tttttaagaa aactagaaaa gaaccttgta atagttatga taaatttgta aaatgtcatc
    16081 atagacggat aataaaagga ggagatgatg aacgttcaga aggtaaatga tgagctaaat
    16141 aaagcacttt ctctcgaata cgctagtgtc atccaaggac ttcagcacag ctttcttgtt
    16201 caggggctct ttagagaatc aatagcaccg atgttaaggg atcatgctga ggaatcgttt
    16261 aagcatgcga agatacttgg ggataagatt gttgccctag gagggcttcc cactgtagag
    16321 ccagcgccag tgaagcaagc atcagattta gtagagatgt taaaacagga ccttgcactt
    16381 gcaagggagg tttatgagca ttatgaatcg cttttaaaaa tggttcagga tgacacacca
    16441 cttcgaatta tggttgagaa ttttgtcgag actgaaaaga aggactgtga agagcttgaa
    16501 aaactccttg agataaggaa gataagggtt aaagacaagg aagtgacttt cagggtgaaa
    16561 tagcgaaatc cttttattgt aaaagcttat agtgttatca cctttaaaaa ataggcggga
    16621 gcgaaatccc gcctattatt ttgtttgtct aattaaattt atggaaaaaa gtattctaag
    16681 ctagtgggaa tcttattacc tttccgaatg tagagacatc atcatgccgt attagcttct
    16741 tttcagcagc attatttgtc ttggaaagaa ttttaaccag tgcttccgct tgactagaat
    16801 caatgggtag ggttttttgc acataccaac ctaaaccctc cccgtagctg agcaagcgta
    16861 tttcaaacct attctcttct tcctttacga taacctctat tatctggtcg ttatcttctc
    16921 catatatact tccgagaagc gcttcggttg cttgagaaat catttattgt tgctccttga
    16981 ataatgttcg tgattaaaat aaattctgga attaaggatt aatttcaaca tattatttga
    17041 ttttgtcaaa ttccttgccc ttagcccaat ggtgttaagt taagcagaga tgttagtgct
    17101 agggcattat ttcgagcgct gcattctttg aaccgcattg tggttcaacg ggaaataaac
    17161 gaacgagaat ggaaataaaa ggttgcgacg aagcaatccc tctgagatta ctcactactc
    17221 ctcggtcttt actcaggaca taggtcgtaa tgacaccgtg tattacgttg gtttgtcact
    17281 atcgctcctt gtaaatttct cgcccttagc cattgtggaa tttattaatt ggataccccg
    17341 ccctctgtaa gcggaatagc ccctaatacc tcaatgtttc cagcgtacat aagacttgaa
    17401 cctgatccta ggactgatcc cccttcaaga taggttttct tcgagtgagg tggtagggtt
    17461 ataggtagaa tccctgtttc ccctccaatt tttgttccat agtttgaata tgaaccggaa
    17521 taataactta cccttacttt gatgtcctta taggctatgt cgctgttatt ttcaatagta
    17581 acatgatgaa tgatagctac ctttccaaag ggaacaaaaa aggaccagtc agtaatatta
    17641 ataaatttct caggatgttg gatcattgac tgtttaatta cagcgtcgtt agcttttcca
    17701 atactataaa aaagactaag tagaattaat gcctttaata atattaactt catttgtttc
    17761 atttttttac cttgagaatt ccatatagct acagggtttc aactgcttgc attgcaatct
    17821 ctcttccagt cattccgaag cgaggaatcc gatactgagt gtcgtcctga gcgtaagcga
    17881 aggatcccaa tataaatcag gagatccttc agtcgctatg ctccttcagg atgacgccat
    17941 gcgtcaaatt gctccgtccc ggagtttatc ctgtgcgaag tcgaagggct ccttgcaatt
    18001 acagtatgga ctgtctgttt atgcgatgat aaatgtgaat ttttcaaaat ttgacggtta
    18061 atattatata gtaaataagg atcctcgtct acttaatgta tcattactcg aatcttatcg
    18121 taagtgcaat gcttaagata taattctgta ggtgatatgt aaatggcaaa gagaataatc
    18181 ttatatactg gaaaaggcgg agttggaaaa actacagtgg cggcggccac tgccctttta
    18241 gcagcaaaaa agggatataa agtccttgtt atctcaacag atgccgccca tagcttagga
    18301 gattcttttg atatggaact aggccctgaa ccgaaagaaa taatgcccaa ccttttcgca
    18361 caagaggttg atgtctatta ttcagttgaa aaatactggg ggaagctaac agaatacctt
    18421 caatcgttac taagatggtt aaaagtagat gagattttga ctgaagaatt tagcatactt
    18481 ccaggaatgg aggaggtatc atcctttctc tgggtctatc accacttcca ggatgagtta
    18541 tacgatgtaa tcatagtgga tagtgcgcca acaggagaaa ccttaaggtt gctttctctt
    18601 cccgatgttg caaggtggtg gatagttaaa gtattcccaa tagaaagaaa ggtggtaaaa
    18661 gtaataagac ccgcgatgaa ggttgtaacg gacatgcccc tccccgagga tcacacatat
    18721 gatgctatag aggaactatt cgataagctt gactctatcc ataatacatt ctctaatcca
    18781 gagatcacct ccattcgctt agtgctaaat ttggagaaga tggttattaa agagactcaa
    18841 agggcttata cttacctgaa cttatacggt tatccggtgg attcagtcat tatcaataga
    18901 actatgccta aagaattgga gcatccattt tttgaggaat ggaaaaaatc ccaacttggc
    18961 tatagagaag aggtggagga gctattcagc ccaattccaa cctttgaagc aaagttgttc
    19021 tcgaaggagg ttttagggat tgaatctctt gaagagtttg gttcaaatct tttttcgaag
    19081 aaggacccga ttcgcatctt tttcaaaggg aaaccttatg aaatcataaa agaagggggt
    19141 tcctaccagt tgcttttaaa actgccattc gtatctaagg aggaaattaa gctctttcaa
    19201 atcaaggatg aacttacaat acaagtcgag aaccatcgcc gtaacatact ccttccaaga
    19261 tttttagctg accttccagt caaagaagcc aaatatcaag atgaaatgct gcggataatt
    19321 tttgagaaaa aagagagaaa gccaaggggc taaagtgtgg atttaacatg agaattctct
    19381 gttgcttgct acaggatttc cacaatgctg tattgccatt gcgaacacgt tcacggagcc
    19441 tgtactgagg aaaccgaagt gctcagtata aactccgtga agtatactct ctttctgtca
    19501 ttcggagcag agcagtttga ctcccttttt gtcattgcga acgaagtgaa gcaatctcgt
    19561 atttagattg cttcgtcccc atcgccttca ctcagggttc ctcgcaatga caatttcgag
    19621 ctgtcattgc aatgaccaaa gagactattt tttatgctga tatcctgcgg cttaccgcag
    19681 ggagcttcat tcaagttttt gcaattttag ctgttttgtt ctattatgca gagctagaga
    19741 aggagaataa cccatgatta aagctcttat tgcatttatg ttagtggtaa cattaataac
    19801 tggaatgtat tcgtgctctg cgatcaccgg taatagtcaa tctgtggctg aagaagagga
    19861 ggaaccacca ggagcggagt atgatccagt attgagacgg tcagagccat ttactggaaa
    19921 aggcgctcca ttatacgatc cattaaagga ggaagagata gagtcgtggg aaaacccgtt
    19981 acaaacggag gagatggaat caaaaccgct tttttaacag tgcgaaatct caaagcaata
    20041 agtcatgcca tatcactttt ttctgaggct tattgaaacc gaattcagct atgtttatct
    20101 ttgtaattcc ctgtaatttg ctacagggtg tcttctgtca ttgcgaggag tgagtgaacc
    20161 gaagccctga gtagaacgaa ggggacgaag caatccaatt gagattcctc actgctcctt
    20221 cgacttcgct caggacaagg ttcgaaatga cacttcgtgt cagcttgctg caattaactt
    20281 cattagaaac tcatagcaga agtgaaagag ggaattaaga aaatgacttt ggagcaacac
    20341 gaaaatgctt aacaaaacaa taaggtcact aattctaggt gttctaataa tcgggattct
    20401 tcagatatac aatagtgtct ctgcggaatc taacccgcca aatataaatt cagaaacaca
    20461 agtaattaaa gacatcaccc ctgacgaagc atttttatta atagagaaga acaaagataa
    20521 ttcgaattta gtaatccttg acgttcgaac gtctaaggaa tttgaagatg gacatataaa
    20581 gaatgcaata aacctcgact tttattccga aacctttaaa gacgatcttg ataaactaga
    20641 taaaaacaag atatacgtta ctcattgtca attaggcggt cgtagcgcta agactgtggt
    20701 gatgatgaag gatttaggct ttgttgaggt ttacaatatc acgggtggga ttgccgagtg
    20761 gaagagcaaa ggactgccaa ccatcaaatc cccctaaatc cccctttgag aaagggggat
    20821 attatttctc cccttaaggg ttaggggatc cctacgataa tttagaaaag aatatattat
    20881 tccaaattaa ccaggagatg ctaatgcttt tcaggggatt ttaattatga tctaactaga
    20941 agggctttaa tgaaatttta ttgacctaac tcgcgacaga tcgaactagc ggcactgaca
    21001 atcaaaacaa ttttcgatac tgtgacggtt tatcaccaat ttaaaaatct aatagtagtg
    21061 aaggaagcca ataaaagaag tcgggttgag caagctaaag gagttacatc aatcttgtta
    21121 ttatcataat agtaaccagt tcccaaagca taacctccag atgagttaaa gtagacacca
    21181 tgagtttgac ggctatcttc cccaaataac atggggtcct ttttaatcaa ctgattagtc
    21241 ttcgtgttga ttctacggat acgattatct gtacaatgtg taacataggt gtagttgaag
    21301 catggatgaa aactcgggcc atggctttgt ttaaatttta gttgtgtcca atctggaatt
    21361 gtttgactta aatcgacctg ataaaccgtg ggtagctaga cagggttcct ggtggaataa
    21421 gagttaacac cttttttcct tagttccggc attccgagta tggcttcgca gaccttgttc
    21481 atgtttctct tcgtcgctta agataccttg taggagagcc ttagcatcag cgtcgtcaac
    21541 caaatcaagc agttccttta ctaatgatcc tacagacgat tcagagtcag cttctctttc
    21601 caagtcagtt gctgacacat ctatcttacc gactaagggt ttcccttgct cgagccagct
    21661 acggatcatt gtgctgatct ccaagcgcct tttcgcatct tcgagcccgt agtcgtaaaa
    21721 cattcgaaca agccagttct ttgtggcagc gatatgcctt gaatcgacat caatttcacc
    21781 tgcctctacg tccgctagct tgttcaacag ttgaatagct tttgctgtct tctctgacat
    21841 atgcgctcct ttgcttctaa aggtctatgc acgatccaca gtgtctcggc ttacatttgc
    21901 aaacctaacg aacaagctca gcgaccgccg caagggacgc gctccggctg caacagtcgc
    21961 gttgaaattc tcccgaaccg tccgactgcc cagcggggcg gcggttcgct gcagcgtaat
    22021 tgttaggcgt catcatcgtg tctcccaccc actctcttgt gctctcttgg tcaactcctc
    22081 gtatcgcgct cgcgccttgc gaagagccaa cgctcggtcg tcagaccaac caatctcgcg
    22141 ctcccaccac tcgtccaact ctcgctgact gcacaactct ccaatcttcc gaatcagctc
    22201 gtcagcctct cgaccataga cttcgagctt gtgcgtgaag aggtcggtca gcggatggtc
    22261 gcctggtttt ccgtttgcca taaggtgtgc ggtgacgcct aacgtattat gaaagggcgc
    22321 cccttcagtg gtagtctgaa gttgcaattt agacaaaaaa ccacttatta aggagggccc
    22381 tatgaacaat gataagtata ttggactaga tgttcatcag tcaagcgtag tcacagc
//
LOCUS       DATDRL010000146         2039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_277642, whole genome shotgun sequence.
ACCESSION   DATDRL010000146 DATDRL010000000
VERSION     DATDRL010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277642"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..1179
                     /locus_tag="VI935_05355"
     CDS             193..1179
                     /locus_tag="VI935_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HLE25059.1"
                     /translation="MSNQILWWVVFNIFVLAMLAIDLGVFHRKAHEIKTKEALIWSAI
                     WITLALLFNLGIYFWRGPQTALEFLTGYLIEKSLSVDNIFIFLLIFSYFRVPALYQHK
                     VLFWGILGALVMRAIFIAAGVTLIQKFHWLIYIFGGFLILTGIKMALEKEKKIHPERN
                     PVLRLFRRFMPITSDYEGDKFLVKHEGRYFATPLFITLLIVETTDVIFAIDSIPAILA
                     ITLDPFIVYTSNIFAILGLRSLYFALAGIMQLFHYLHYGLSAILVFVGAKMLLAYIYK
                     IPVDIALGVVAGFLLISVIASIIRPREAESILTPADSQGTEPTRVPQIKDAP"
     gene            complement(1430..2005)
                     /locus_tag="VI935_05360"
     CDS             complement(1430..2005)
                     /locus_tag="VI935_05360"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009591892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HLE25060.1"
                     /translation="MILMIDNYDSFTYNLVHYLGELGEKVLVFRNDKITIEDVGKLNP
                     DMVVISPGPCTPKEAGISVDLIKEFAGRIPILGVCLGHQSIGYAFGGNIVRAKRLLHG
                     KTSQIYHDGKRIYEGVPTPFEATRYHSLLVEGSSLPDELEVTAWTNEDEIMGIRHKEY
                     LIEGVQFHPESILTKHGKDLLRNFIRIAKSK"
BASE COUNT          563 a          423 c          413 g          640 t
ORIGIN      
        1 cttttcttcc accggatgtt atagagaaga caacggagaa atatattgaa gctcttcgga
       61 ggcttacaca gaactgaatt gaaggaagaa tattgagttg aattaaaaat taaaagcttt
      121 ttcaaccagt ctgacattag attagatgca gtggcaatac tagtaaacta atcaaagaaa
      181 aggagagtct tgatgtcgaa ccagatccta tggtgggtgg tctttaacat ttttgtgctc
      241 gcgatgttgg caatagatct tggggttttc catcgcaagg cccacgaaat aaagactaaa
      301 gaagcgctta tatggagcgc tatctggatt actctagccc ttctcttcaa tctgggaatt
      361 tacttctggc gcggtccgca gacagcgttg gaattcctga ccggttatct gattgagaag
      421 tctctgagcg ttgacaatat cttcattttt ctcttgatct tctcctactt tcgagtgccg
      481 gctttatacc aacataaggt gcttttttgg ggtattctcg gagcgctggt tatgcgtgcg
      541 attttcattg ctgcgggtgt gacattgatt caaaagtttc attggttgat atacatcttc
      601 gggggtttcc taattttgac tggtataaaa atggctttgg agaaagaaaa gaaaatacac
      661 ccggaaagaa atccagtgct tcgcctcttt cggcggttca tgccgatcac aagcgactat
      721 gaaggcgata agtttttagt aaagcatgag gggcgttatt tcgctacgcc gttgtttatt
      781 acgctgctta ttgtagaaac aacggatgtg atttttgcaa tcgactcgat tcctgccatt
      841 ctggctatca cattagatcc atttattgtg tacacttcca acatatttgc aattttaggg
      901 ttgcgatcgc tctactttgc attagctggg attatgcaac tgtttcacta cctccattac
      961 gggctttctg ccattctcgt cttcgtgggg gctaagatgc tacttgctta tatctacaag
     1021 attccggttg acattgcttt aggtgtggta gctggttttc tccttatctc tgtgatagct
     1081 tcgattattc gaccacgtga ggcggagagc atcctaacac cggctgattc ccaaggaaca
     1141 gaacccacac gtgttcctca aattaaggat gcaccgtgag ccaatagcct atatcgagaa
     1201 catggcttca ttctatgaaa gcagaacttt gaattagtat gctcgaagaa acgcttcttt
     1261 ttcctatcca aagcttgttt atgtggatgt tattttaaaa gagaaaggga tgattcgata
     1321 ggactgacga actagactct aatttacagt gttgactggc atataataaa aacgagagaa
     1381 aacaatccga atctaccttt ggattagaaa aagcctacct actacaaaac tatttacttt
     1441 ttgctatcct aataaaattc cttagaaggt cttttccgtg ctttgtgaga attgactctg
     1501 gatggaactg tactccttca atcaaatact ccttatgcct tattcccatt atctcgtcct
     1561 cattagtcca ggctgtcact tcgagctcat ctggaagtga actaccttca actaaaagcg
     1621 aatggtacct cgtggcttca aagggcgtag gcacgccttc ataaatcctt ttaccatcat
     1681 ggtaaatctg agaggttttt ccatgaagta gcctttttgc ccttactatg tttccgccaa
     1741 acgcataacc gatagactga tgaccaaggc aaaccccaag gattggtatt cgtccagcaa
     1801 attctttgat taagtctaca gatattcccg cctctttagg cgtacagggg ccaggagaaa
     1861 tcacaaccat atcaggattt aatttgccta cgtcttcaat cgtaatttta tcgtttctga
     1921 aaactaaaac cttttcacca agctcaccaa gataatggac gagattatat gtaaatgaat
     1981 cgtagttatc tatcataaga atcatttttc tagccccatc ttcatactag aataaattc
//
LOCUS       DATDRL010000147        14870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2913270, whole genome shotgun sequence.
ACCESSION   DATDRL010000147 DATDRL010000000
VERSION     DATDRL010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14870
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2913270"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1918)
                     /locus_tag="VI935_05365"
     CDS             complement(188..1918)
                     /locus_tag="VI935_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLE25061.1"
                     /translation="MYRNPADAIKEHVSNAIDEHLKAKASGKALQVCKVIFTMEKDRV
                     IIEYPYGMSKKEFESALQRVADSAKKSIDIKQIGQLGIGIFSFLQFGRKCTFLSKEDG
                     ADETIKVTLREGSDDAEFETALKRERLLERGIKISISELIFDPTKTRGPLSPERLQKL
                     FAERFDTYLKDGSLEIIIRSKGVSYTVKPLKIELPKIGEDYKNWPFKRDGTKKFSLEL
                     YFDPSRKGTVSMRHMGVAIVDDIKTLFAYGLEESIYTSGYVKGFIDADFLKPLPARTG
                     FEENEDWISLLDELDQLRSSIEAEVEYHKQEEAEKKLTEIQKKAIELAREILNTEQFK
                     DLELLEGLGRKPPEPILPPKGFEFIPSSIRIEPGEDGSLPLKASIPKVVPDNSLVRLS
                     INDPSSVELKTNSLFLKASEADKNSVVTAYVQFAAKLKTTVPVILTAISGNLKAEAHI
                     RVAELEQKREPKAPGVQKEGPRITYEEWPFEEGPSKHSRYLSRKIQINELNRNYIREV
                     KMAKSEQSPLAYAALMIGKETIAYNDKTGAVDEYLEKLLSFHFELKNRLIGTSPSVVQ
                     RPRGRPRKSI"
     gene            complement(1924..2637)
                     /locus_tag="VI935_05370"
     CDS             complement(1924..2637)
                     /locus_tag="VI935_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLE25062.1"
                     /translation="MSKGSVEQLLIELDNFSDTKNLSRREMAQKLNIPYETFRKWFHN
                     GKGKKKPSPAYIEKIEKFLESQKETETYWKELWIKILKWWETQHRYSTIRELADEIGW
                     DAQSLITYFQNKELPPKLVVEKIAKTLGVETPALDSVFQEAQRKTEKVKHVLLFLEEE
                     LRWFRDSSKETREIFRKELNPADIGYVSSLLTMLGDEDKFRRWLTLTTNRFNFFKKRG
                     EQKRRKFQLSFTRQMPLNG"
     gene            complement(2910..3242)
                     /locus_tag="VI935_05375"
     CDS             complement(2910..3242)
                     /locus_tag="VI935_05375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006529572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HLE25063.1"
                     /translation="MMVNPGDVVTVDFPGALGVKRRPAVVVSTQTYHTTRPDVVLCLL
                     TSQTAGATGPTDYILQDWSSAGLHSPSALRAFLATLPATSIIVIGHLSDRDWQEVQER
                     LRIALAVT"
     gene            complement(3256..3462)
                     /locus_tag="VI935_05380"
     CDS             complement(3256..3462)
                     /locus_tag="VI935_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25064.1"
                     /translation="MLTRRQAEEILRDLTALPPEKVAEVQDFILFLKKHYGYEKALDE
                     SNAWTEEDLRDLIAAVLNHADQTL"
     gene            complement(3638..4051)
                     /locus_tag="VI935_05385"
     CDS             complement(3638..4051)
                     /locus_tag="VI935_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HLE25065.1"
                     /translation="MKYWLVKSEPFKYSWEMFLKDGSTYWDGVRNYQARNNLKNMKNG
                     DLVLFYHSNEGLEVVGIAKVIKEAYQDPTTNDQRWVVVDLEPFETLKKPVPLDEIKKD
                     KRLKKIALIKQSRLSVMPLTKEEFETIVTKERGKE"
     gene            4434..5072
                     /locus_tag="VI935_05390"
     CDS             4434..5072
                     /locus_tag="VI935_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25066.1"
                     /translation="MKLFNLFLFSIALFFILSCSVPIQRAEKGPQRIALKPAEVDIKT
                     IVLVPFSLDTEVTSGKELYLTDIMYNELISKILKVSIVPLEISTSEYSRIKTENPDLP
                     NSEVAVKVGKDLGAQAVIVGTIFAYTEREGGELGVSSPASVAFGVDLVNTATGEKIWE
                     NYFAETQKPLFENVAEVGKFFKRKGKWISADELVKEGVIEVVDNLNKFLEQN"
     gene            5073..5795
                     /gene="hisA"
                     /locus_tag="VI935_05395"
     CDS             5073..5795
                     /gene="hisA"
                     /locus_tag="VI935_05395"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HLE25067.1"
                     /translation="MLIIPAIDLKGGKCVRLLKGEEGTETVFSENPLETARKWEDSGA
                     RLIHVVDLDGAFSGEPRNFEIIRDIVNTVSTDVQIGGGIRNINTVEKYISVGVRRVIL
                     GTSAIQDRKFLIEVCDSFPERVGVGVDTKMGKIAVKGWKEVIDLHAEDFLKDLNSVGV
                     SLVIHTDVDRDGTMRGVNLNAVDKFVKSSPIPVISSGGISSMEDIEKLSSLKEFGLVG
                     IILGKSIYTGKIDLRKAIEKFS"
     gene            5901..6659
                     /gene="hisF"
                     /locus_tag="VI935_05400"
     CDS             5901..6659
                     /gene="hisF"
                     /locus_tag="VI935_05400"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HLE25068.1"
                     /translation="MLAKRIIPCLDVKDGRVVKGVKFLNLKDAGDPVEIATYYSKEGA
                     DEITFLDITASFEERKTMIDVVERTAGEVFVPLTVGGGVRTLEDIRQLLLAGADKVSI
                     NTAAVKDPEFVKRASDRFGSQCIVVAIDAKRVSNSEWEVFTHGGRNPTGIDAIEWAHK
                     MEDYGAGETLLTSMDMDGTKAGYDIELTKAVSRSVGIPVIASGGAGNLQHIYEGLTFG
                     EADAALVASIFHYGEYSIQEAKEFLRKKGVHIRM"
     gene            6808..7857
                     /locus_tag="VI935_05405"
     CDS             6808..7857
                     /locus_tag="VI935_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HLE25069.1"
                     /translation="MTIREELEEIERKVLSPLATLSSETKGRIKLEDPCPIRPAFQRD
                     RDRIIHSKSFRRLKHKTQVFLAPTGDHYRTRLTHVIEVSQIARTISKALRLNEDLTEA
                     IALGHDLGHTPFGHAGEAALNEIFPGGFKHVIHSLRVVDVLERDGQGLNLTYEVRDGI
                     SKHSKGMGSLDNPKYRPETMEGQVVRLSDLVAYANHDVDDAIRAGIITIDDVPRECVN
                     VLGKTNSERINRMVTDIILETKKLGEKNIVMSKKVEESIIELRSYLFDTVYMNEKIRK
                     NFLKATKLMKELYEYLCSYPEEFWKFYGKTHGNSEPIERAVCDFIAGMTDSYAISIYE
                     KIFLPKRWQGDLSTF"
     gene            7932..8780
                     /locus_tag="VI935_05410"
     CDS             7932..8780
                     /locus_tag="VI935_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HLE25070.1"
                     /translation="MSNHDSLTTKNEVGEIALIKLGSVPFLNVKPLVFPLEERLVQHD
                     FEISYTSPSNLSTLLFDKNVDLGLMPVAELIKRGNYRIVPNISISSYGKVDSVILLTR
                     SEIKELKTVAVDVRSQSSTALLRIILEIFNNLSPVYIRREADEKFLNGVDGGMLIGNI
                     GLKLRYFPPDGYMVFDLGEIWTNETGLPFVYAVYAVNKGIKLGRNLQALETAKSIGLK
                     IVKKITKIESEKIGLGEEICLRYLTERIRYDLGEKEISGVLNYAKLLSDLDENRRIPE
                     IKFYSE"
     gene            8859..9677
                     /locus_tag="VI935_05415"
     CDS             8859..9677
                     /locus_tag="VI935_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012940624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HLE25071.1"
                     /translation="MKLGHSPDADDAFMFYGMASGKVVSDKIDFIHVIEDIQTLNRRA
                     LNGELEVTAVSAHAYLYIQDKYRILSCGASMGEGYGPIVVAKERFDDLNGKKVGVPGR
                     LTTAFLLLSLYADGFIPVDLPFDKVMEAVRDGEVEAGLIIHEGQLTYKDFGLTMIFDL
                     GETWAKDTSLPLPLGINTVRRDIEQGLQKEILRVHRESIEYGLAHKDEALEYALRFGR
                     GMKKDLGKKFVLMYVNQYTRNMGEKGKKALEFLFSKAFQKGVIKDKARLDIQEG"
     gene            complement(9774..10835)
                     /locus_tag="VI935_05420"
     CDS             complement(9774..10835)
                     /locus_tag="VI935_05420"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HLE25072.1"
                     /translation="MISEIRKLFVYGTFLQGEPRNYYLNNCKLIQGLEIPGKLYNTNR
                     GYPAVLLDGSSKETVVGEIYNLCEDSNKILKELDEVECTRFGLYRRKDLKHKGHHFYL
                     YEAGEQLRGLLNKENSINSGNWRRQGSFALKDPVEFALTFEKEQQERYREFPPEDSSG
                     MIFLRGDAPILITAPHATRHLRMNKQKQQEEYTGAISVILHSLIGTHALYTHWASKID
                     PNFYDDAPFKRKLAKVVRKFGIKFVLDLHGTKVQRNKDLYPGVGNNTEFLIGNDSYLH
                     KLEDSAKSYGLVLGSLNVFPASIQMTITKFVATKIGTPAMQIEINERLRDPEGNPSQF
                     ERLVRLLTDYIYSISGPSF"
     gene            complement(10828..12084)
                     /locus_tag="VI935_05425"
     CDS             complement(10828..12084)
                     /locus_tag="VI935_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLE25073.1"
                     /translation="MEKLLITAENFLPISSPPLIKGSILIVDGKIREIGKASKLKKKY
                     QGIPTLELGNGILLPGFVNAHTHLELGWIGKRIGKFSGFISWLEHIIKARMADTSREE
                     IETSVEEGIKMLLQCGVTTIGEISSYGGLDKPLLKKSGMRTVLFPELFDWHGDFWEIF
                     SFDKDETFEERPFPHATYSCSPEFLKKVWRFNHQRGIPLGIHLAESPDEVKFIRGEVN
                     GFEKKIFPLIGKESFVRDKSDTPFQYLKGLKFFNGTKVTAVHMVQILPEEVEEINKHD
                     IGIVLCPRSNFFLKVGAPPLKYLADLERIGIGTDGLSSNFNLDFFEELRFFYLTFGKK
                     LGEKAPFFTIYLATLGGARALFLDERIGSIDKGKEADLIFLSPKNPFPDPYLSVISCC
                     KEDVKLVLVKGKVVSSKTEIPVNIHD"
     gene            complement(12306..13175)
                     /gene="dapF"
                     /locus_tag="VI935_05430"
     CDS             complement(12306..13175)
                     /gene="dapF"
                     /locus_tag="VI935_05430"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HLE25074.1"
                     /translation="MNKFVKSHGLGNDYIILDRSNISFELNPDVIKLICHRNYGIGSD
                     GILLLVPSNKTDFGLKIFNPDGSSAEKSGNGLRIFAKFLYEHEYTKKELFNIDTPGGI
                     VTAELEVINNIVSFVIVEMGEATFRSKLIPVEGEDREVVGEEIRVNGEILRFTAVSVG
                     NPHCVFFVNELDEGYARKLGPILETHPLFPNRINVQFAHVESRNKVNILIWERGVGYT
                     LASGSSSCAVAAACFRNGLTDKYVTVSMPGGELQIQIRDDWSIKMRGPVEEVYVGTIS
                     QDLLEKINKFSSV"
     gene            complement(13377..13778)
                     /locus_tag="VI935_05435"
     CDS             complement(13377..13778)
                     /locus_tag="VI935_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLE25075.1"
                     /translation="MKAREIMSEDIISVDENTSVKEAAQIMAKHEIGALIIQTPKPTF
                     GIFTQNDLLVRVVAEGKNPEKTKIRDVMTESAQCAQAEDSVDELVRIMYEENVRYLPV
                     MDGRKLVGIISATNLFKVIFRGAEGYQEEVV"
     gene            complement(13867..14070)
                     /locus_tag="VI935_05440"
     CDS             complement(13867..14070)
                     /locus_tag="VI935_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HLE25076.1"
                     /translation="MAVARISEVIGSSSKSWEDAVQNALKRANKTLRGLTGIEVTKMN
                     AHIEKGKITEYRAHVKITFILEE"
     gene            complement(14369..>14870)
                     /locus_tag="VI935_05445"
     CDS             complement(14369..>14870)
                     /locus_tag="VI935_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HLE25077.1"
                     /translation="FDVDKFIDAYGNVPPEFLQTGFLIMKPIQNMVSKYVSFYENIDN
                     EDFVENFLAMEKWINDNIPVAGEVFREFIKLCYQENLLIENKLRINGKLVELKKIGCS
                     TLNLIAEHDHLVPPASSKVFNDLISSKDKETVIYPTGHIGLSVSSKALKDLWPRVADW
                     LIARSK"
BASE COUNT         4287 a         2813 c         2973 g         4797 t
ORIGIN      
        1 gtaaataaga gtgatttggt tgggcgaacc gtcggctcgc ccctacttta tcaataaaag
       61 cgaatttcgc ctgcagtata tctaaaagag agactttttc tcaacatcgt gcctttatta
      121 tatgttagac gacctatttg cagagtatct cctctaaagt atttctacat catttaaaaa
      181 atgataatca aatacttttt cttggtcgtc cacgaggtct ctggacaaca ctaggcgaag
      241 tcccgattaa tctatttttt aattcgaaat gaaaagaaag tagtttttcg aggtactcat
      301 cgacagctcc agttttatca ttataggcta tggtctcttt cccgatcata agcgcagcat
      361 aggcaagtgg tgattgctct gattttgcca tctttacttc tcgtatataa ttgcggttca
      421 actcattaat ttgaatcttc ctcgagaggt atcggctatg ttttgatggt ccttcctcaa
      481 aaggccattc ctcataagta atccttggac cctctttttg tactccagga gctttaggct
      541 ctcttttttg ctcaagttct gcaacccgga tgtgagcttc tgcttttaaa tttcctgata
      601 tagcggtcaa tattactgga acggtagtct ttaatttagc agcaaattga acataggcag
      661 taacaacgct attcttgtcc gcttctgatg ctttcaaaaa caggctgttt gttttcaact
      721 ctaccgagga agggtcgtta atgctgagtc ttaccaaact attatctggg actaccttgg
      781 ggatggaggc tttcaatgga aggcttccat cttcccctgg ttctattcta atcgaagagg
      841 gtataaactc aaatcctttg ggcggtaata ttggttcagg aggttttcgt cccaatcctt
      901 ctaatagttc taagtccttg aattgttctg tatttaagat ttctcttgcc aattcgatag
      961 cttttttctg aatctcggtt aatttctttt ctgcttcttc ctgtttatga tattcaactt
     1021 ctgcttcaat tgatgagcga agttggtcga gttcatcgag cagacttatc caatcttcat
     1081 tttcctcaaa tcctgttcta gcaggaagag gttttagaaa atcagcgtcg atgaatccct
     1141 ttacgtatcc gctcgtataa atgctttctt ccaacccata agcaaacaag gttttgatat
     1201 cgtcaacaat agcaacgccc atatggcgca tactaactgt cccctttcga gatgggtcaa
     1261 aatataattc aaggctgaat ttcttggtgc catccctttt aaatggccaa tttttatagt
     1321 cttcgcctat ttttggtagc tctatcttta acggctttac agtataggaa acccctttag
     1381 accgaatgat aatttcgagt gagccatcct tcaagtaggt gtcaaatctt tcagcaaata
     1441 gtttttgaag cctttctgga gaaagggggc cccttgtttt agtaggatca aatattagtt
     1501 cagaaatact aattttaatt cctcgttcta ataatctttc ccttttaaga gcggtctcaa
     1561 attctgcatc atcagagcct tcccgaagtg taacttttat agtttcgtct gctccatctt
     1621 ccttagaaag aaaggtgcat ttcctcccga attggagaaa actaaatatt ccgattccca
     1681 gttgccctat ttgttttata tcaatggatt ttttggcaga gtcagctact cgttgaagcg
     1741 ctgattcgaa ctctttttta gacataccat agggatactc aattattacc ctatcctttt
     1801 ccatagtgaa gattaccttg caaacttgaa gtgctttacc agaggctttt gctttaaggt
     1861 gctcatctat tgcattgctt acatgctcct ttattgcgtc agcaggatta cgatacaaac
     1921 tccctaacca tttaagggca tttgccgtgt gaagcttaat tggaactttc ttcttttctg
     1981 ctcacctctc tttttgaaaa aattgaacct gttagtagtt aaagtgagcc atcttctaaa
     2041 tttgtcctca tcacctagca tggtaagaag acttgaaacg tatccaatat cagcaggatt
     2101 taactctttc cgaaagatct ctcgggtttc tttagagcta tctcgaaacc atctgagttc
     2161 ttcttccaaa aataataaaa catgtttcac tttttccgtt tttctttgtg cttcctgaaa
     2221 tacggaatct aaagcagggg tctcaacacc aagagttttt gcaatctttt ctaccacaag
     2281 ttttggaggc aattctttat tctgaaagta ggtgataaga ctttgagcat cccaaccaat
     2341 ttcgtctgct aattctctaa ttgtagaata ccggtgttgt gtttcccacc atttcaaaat
     2401 ttttatccag agttctttcc aatatgtctc ggtttctttc tgagattcaa gaaacttctc
     2461 aatcttttcg atatacgctg gagaaggttt tttcttccct ttgccgttgt gaaaccactt
     2521 tctaaaagtc tcgtaaggga tattcagctt ttgtgccatt tctcttcttg aaaggttctt
     2581 tgtatcggaa aaattatcta actctattag taactgttcc acggatcctt tgctcacctt
     2641 atctcacctt tcattccttc attatggttt aagaatagat cccaaagcaa aatgttgact
     2701 aactaattcg catacgaagt tcgctttttg tccaattttg gaagtatatt cgaacttcag
     2761 atatagattc ctaaattttt caactccttg ttatttactc ataattctat aaaaactctg
     2821 attttttaca atagctttgt ttattaaaca agcagacaat ctttggccat aactaagcaa
     2881 agtgtaaatt tgctgttatc tgccgttcct tacgtcaccg ccaatgctat acgaagtcgc
     2941 tcttgcacct cttgccaatc gcggtccgac aggtgtccta tcacgataat gcttgtagca
     3001 gggagcgttg ccagaaaagc tcggagtgca gaaggactgt gtagaccggc agaggaccaa
     3061 tcctgtagaa tataatccgt aggccctgtt gctccggcag tctgactggt tagtaaacag
     3121 agtacaacgt cgggacgtgt tgtatgatat gtttgcgttg aaactacaac agcggggcgc
     3181 cgctttactc ctaaagctcc tgggaaatcc acagtaacga catcgcctgg gttgaccatc
     3241 atactttgcc tgttctcaga gggtctgatc tgcgtggtta agtacagctg ctataagatc
     3301 gcgaaggtct tcctcagtcc aagcgttact ttcgtcaaga gccttttcat aaccgtaatg
     3361 ctttttcagg aagagaataa aatcctgaac ctctgcaacc ttttcaggtg gtaacgcagt
     3421 tagatcacgt aagatttctt cagcttgtct tctggtcaac ataagtcagc ctccttattg
     3481 ggttgagaaa tagcagataa ctcgttcatc tgtaattcgc tttttatccc attttggaat
     3541 tatatctgaa tcttggatat acgtttaaat gttttaagtc ctttttattt accatctatt
     3601 ttataagaac tctaattttc ccgcaatatt tattatttca ttccttccct ctttccttag
     3661 ttactatagt ttcaaattct tcttttgtaa gaggcatgac ggagaggcgg ctttgtttga
     3721 taagggcaat tttcttcaag cgtttgtctt tttttatttc atctagtggg acaggctttt
     3781 ttagagtctc aaaaggttct aggtctacga ccacccaccg ttggtcattt gttgttgggt
     3841 cttgataggc ttctttgatc acctttgcaa tgcctacgac ttctagtcct tcattgctat
     3901 gataaaaaag caccaaatcc ccatttttca tatttttcag attatttctt gcttgataat
     3961 ttcttactcc atcccagtag gtcgaaccgt cttttagaaa catttcccag gaatatttga
     4021 atggttcaga ttttactaac cagtatttca tgccttttat tatagaatgc caaaccgaag
     4081 gttttcaagt tcaagcgcat acaaatgtct ttcggtttaa ctcagtgcat gtttcgttca
     4141 gtataaactc cgtgaagtaa tctgacgcaa gatgtcattc cgaatacatt gaggaatctc
     4201 aatcagattg cttcggtcgt ttcactccct cgaaatgaca ccattttttc tgtcattgcg
     4261 aacgaagtga agcaatctcg ccttttcagg attgctttgt ccccttcgct ttgctcaggg
     4321 ctttggttca ctcacttctc gcaatgacca aaagggtttt ctgcttatat gacgatgcct
     4381 gtaggttgat acaaggttct ttatttaacc ctatattcct gtatatttat aacatgaaat
     4441 tatttaatct ctttttattt tctatagctc tcttttttat actttcctgt agtgttccga
     4501 tacaaagagc ggagaaaggt ccccagcgaa tcgctctaaa accggctgag gtagatataa
     4561 aaacaatcgt attggttccg ttttccttgg acacggaagt tacatccggt aaagagctat
     4621 atttgactga tattatgtat aatgagctaa tatcaaaaat attaaaagta agtatagtcc
     4681 ctcttgagat ctcaacctct gaatattcaa ggataaaaac tgaaaatcca gacttgccaa
     4741 acagtgaggt ggcagtcaaa gttggcaagg acttaggggc tcaagctgtt atagtgggaa
     4801 ccatatttgc atatacggaa agagaagggg gcgagttagg agtgtcctct cctgcatctg
     4861 ttgcttttgg tgttgatttg gtaaatacag cgactgggga aaagatctgg gaaaactatt
     4921 ttgctgagac tcagaaacca ctattcgaga atgtcgctga ggtaggcaag ttttttaaaa
     4981 ggaaagggaa atggatatcg gctgatgaac ttgtaaaaga aggagtaata gaggttgtgg
     5041 acaatctcaa taagtttctg gagcaaaatt aagtgctaat tatccctgct attgatttaa
     5101 aagggggaaa gtgtgtaaga ctattaaagg gggaagaggg aactgagaca gttttttctg
     5161 agaatccgtt agaaactgca agaaaatggg aagattcggg agccagactc attcatgttg
     5221 ttgaccttga tggtgcattc tcaggcgaac ctagaaattt cgagataata agggatattg
     5281 ttaatactgt ttctacagac gtgcagatag gtgggggaat aaggaatatt aacacagttg
     5341 aaaaatatat aagtgtgggt gttagaagag ttatactagg aacatctgca attcaagata
     5401 ggaaattctt gatcgaggtg tgcgacagct ttccagaaag ggtaggagtg ggtgttgaca
     5461 ctaagatggg aaagattgct gtaaagggat ggaaggaagt gattgatttg catgccgaag
     5521 attttcttaa ggatttaaac tcagtaggcg tatctttagt tattcacact gatgttgatc
     5581 gtgacggtac aatgcgcggg gttaacctaa acgctgttga caaattcgtt aagtcttcac
     5641 cgattccggt aatatcatca ggcggcatat catcaatgga agatatagaa aaactttcct
     5701 ctttgaagga gttcggtctt gtaggaatta ttcttggtaa atcaatttac actggcaaaa
     5761 tcgatctcag gaaggcaata gagaaatttt cgtaactctg gcaacagaga acacggaaaa
     5821 cagtgaggaa aaaacaaaaa tatatttttt taattttttt tctctgtgtc ctctgtggct
     5881 aaacagttcg aggttacttt atgcttgcaa aaagaattat tccctgcctt gatgtaaagg
     5941 atggaagggt tgtaaaggga gtgaaattct taaatctcaa agacgcaggg gatccggttg
     6001 aaattgctac gtattatagc aaagagggtg ctgacgaaat tacatttctt gatattacag
     6061 cttcctttga ggaaagaaaa acaatgattg acgtagtgga gcgtacagcg ggggaggtat
     6121 ttgttcccct tactgttgga ggtggggtta gaacattaga ggacataagg caacttcttc
     6181 tagctggagc ggataaggtg tccataaaca cagcggctgt aaaagatcca gagtttgtta
     6241 agagagcatc agatagattt ggaagtcagt gtatagtcgt agccatagat gcaaagaggg
     6301 tttccaactc tgaatgggaa gtctttacac acggtggaag gaaccctaca ggaatagatg
     6361 ctatagaatg ggcacataaa atggaggact acggagcggg agaaaccctt cttactagta
     6421 tggatatgga cggtaccaag gcggggtatg atattgagct tacaaaggct gtatcacggt
     6481 ctgttggtat tccagtgatt gcttcaggag gtgcgggtaa cctacaacat atttacgaag
     6541 gactgacttt tggagaggca gatgcggcat tagttgcctc aatttttcat tacggtgagt
     6601 attctattca agaggcgaag gagtttttaa gaaagaaagg ggttcacatt agaatgtaaa
     6661 tcgtggttag tgaatagtgg ctcgtgattc ataaattgtt gttcgaatca cgaaccccga
     6721 accacgaaaa ccgggtctta accacgtgct cgcaatgata acaattttgg atagttagga
     6781 tgttattgat ttataggggg ctctaatttg acaatacgag aagaactaga ggagatagaa
     6841 agaaaggtgc tttcccccct tgcaacatta agttctgaaa ccaaaggcag aattaaacta
     6901 gaagaccctt gtcccatccg acctgctttt caaagagaca gggacagaat aatccactcc
     6961 aaatctttcc gaagactcaa acataagacc caggtctttc ttgctcctac aggagatcac
     7021 tatagaacaa ggcttactca tgtaatagag gtttctcaga ttgcaagaac aatctccaag
     7081 gctctaagac ttaacgaaga cctaactgag gcaattgcct tgggacatga tttgggtcat
     7141 acaccttttg gtcatgcagg agaggctgca cttaatgaaa tatttcctgg ggggttcaaa
     7201 cacgtaatcc acagccttcg tgtcgtagat gttcttgagc gagacggtca gggtttaaac
     7261 ctgacttacg aagtacggga tgggatttcc aaacattcaa agggtatggg atcgctcgat
     7321 aatcctaaat atcgtcctga aactatggag gggcaggttg taaggctatc ggatttggta
     7381 gcctatgcga atcacgatgt agatgatgca ataagagcgg ggattattac aattgatgat
     7441 gtgccaaggg aatgtgtaaa tgttcttgga aagacaaatt cggaaaggat caatagaatg
     7501 gttacggata ttatcttaga gactaaaaaa ctcggtgaaa agaatatagt aatgagtaaa
     7561 aaagttgaag aatctataat tgaactcagg agttacctct ttgacacagt ttatatgaat
     7621 gaaaagataa gaaagaattt tcttaaagct acaaagttaa tgaaagagct atacgaatat
     7681 ttatgctctt atccggaaga gttttggaaa ttttatggaa agacccatgg aaactcggaa
     7741 ccaattgaac gcgctgtgtg tgattttatt gcaggaatga cggattcata tgcaatttca
     7801 atttatgaaa agatattttt accaaagcgt tggcaggggg atttaagcac tttttaagtg
     7861 tgtttcgtgg atcgtgtctc gtggctcggt atttatttag gttttgttgt ttgtaaagag
     7921 agttcataaa gatgtcgaat cacgattcac taaccacgaa aaacgaagta ggagaaattg
     7981 ctttgattaa actaggctct gtgccgtttt taaatgtaaa accacttgtt tttccactgg
     8041 aagaaaggtt agtgcagcac gattttgaga tttcatacac ctctccttcg aatttgtcca
     8101 ccctgctttt tgacaaaaat gtagatttag ggctcatgcc agttgctgag ttaattaaaa
     8161 ggggaaatta tagaattgtc cccaatatct cgatttcgtc ttatgggaag gttgatagtg
     8221 ttattctgtt aactagatcg gagataaagg agcttaaaac agttgcagtt gatgtaaggt
     8281 cccagagctc aacagcactt ctaagaataa ttttagagat ctttaataat ctatccccag
     8341 tttatatacg aagagaagct gatgaaaaat ttctgaatgg cgtggatgga gggatgttaa
     8401 ttggcaacat aggtcttaag ctcagatact ttccgcccga tggatatatg gtttttgatc
     8461 tcggagagat atggactaac gaaacaggtc ttccgtttgt atacgctgtc tatgccgtga
     8521 ataaaggaat taaacttggt agaaatctcc aggcattaga aacggcgaag tctataggat
     8581 taaagatcgt caagaagatt actaaaatag aatcagaaaa aatagggctc ggtgaagaaa
     8641 tctgtttaag atacctaaca gagagaatta gatatgatct cggtgagaaa gaaataagtg
     8701 gggttttaaa ttacgccaag cttctttcag atttagatga aaatcgaagg attccggaaa
     8761 taaaatttta ttctgagtga gaattagtga atcgtgattc ctgtatcgtg aatcgtgtat
     8821 cgaaccacga ggagataaat aatttgagga aattctcaat gaaactaggg cacagcccag
     8881 atgctgatga cgcatttatg ttttacggga tggcaagcgg aaaggtggta tccgataaaa
     8941 ttgacttcat tcatgtgata gaggatatac aaactcttaa tagacgtgct ttaaatgggg
     9001 aattagaagt aactgcagtc tctgcacatg cctatcttta catccaagat aaatatagaa
     9061 ttctttcatg cggtgcaagt atgggggagg ggtacggtcc aattgttgtg gcaaaagaac
     9121 gatttgatga tctaaatggg aaaaaggttg gagttcctgg aagactcacc acagcttttc
     9181 ttctcttaag tctttacgcc gatggattta tccctgttga tctgccattt gataaagtaa
     9241 tggaggctgt cagggatggc gaggttgaag ctggactcat tatacatgag ggacagctta
     9301 cgtataaaga tttcggacta acaatgattt ttgatcttgg agaaacgtgg gcaaaggata
     9361 cttctctacc tcttccccta ggaattaata ccgtaagaag agatattgag caaggcttgc
     9421 agaaggagat tttaagagta catagggaga gtattgaata cgggttagct cacaaggatg
     9481 aagccttgga atatgcgctt cggttcggga ggggaatgaa aaaggattta ggaaaaaaat
     9541 ttgttcttat gtatgttaat cagtatacaa gaaatatggg ggagaaaggg aagaaggcct
     9601 tggaatttct ttttagcaag gcttttcaaa aaggggttat taaagataaa gctagactgg
     9661 atatacaaga aggttagcgg cttgtcacta tgaaagtaag agattgtcat tctgagccga
     9721 aggcgaagaa tctcccttta aaccaagaat gagattcttc gtcgctgcgc tcctcagaat
     9781 gacggaccgc taatagagta gatatagtct gtaagaagtc taactaacct ttcaaattga
     9841 gaaggattac cttccggatc tctaagcctc tcattaatct caatctgcat tgctggtgtt
     9901 cctatctttg ttgctacgaa tttagttata gtcatttgaa ttgaggctgg gaaaacgttg
     9961 aggctgccaa ggactagacc atatgatttt gcagaatctt caagcttatg aaggtaagaa
    10021 tcatttccta ttaggaattc tgtgttattt cctactcctg gatataggtc cttattcctt
    10081 tgaacttttg tgccatgtaa atcaagaaca aacttgattc caaattttct tacaaccttt
    10141 gcgagcttcc ttttaaaagg ggcatcgtca taaaaattgg ggtcaatctt tgacgcccaa
    10201 tgtgtgtaga gtgcatgtgt gccaattagt gaatgaagaa ttacagaaat tgctcctgta
    10261 tattcttctt gttgtttttg tttattcatc cgcaaatgcc ttgtggcatg tggcgcggta
    10321 ataagaatag gcgcatctcc tctaagaaat atcattccac ttgaatcctc cggaggaaat
    10381 tctctatatc gctcttgctg ttctttttcg aaggtaagag cgaattcaac tgggtcctta
    10441 agtgcaaacg atccttgacg acgccagttt ccagaattaa tcgaattttc tttattaaga
    10501 aggcctctta attgttctcc agcttcgtag aggtaaaagt gatgtccctt atgttttaag
    10561 tcctttcttc tgtagagacc gaatctagta cattcaactt cgtctaattc ttttagtatc
    10621 ttattagaat cttcacatag attgtaaatc tctccgacta cggtttcttt agaactccca
    10681 tcgagcaaaa cggctggata tcctctatta gtgttgtaaa gcttgcccgg tatttccaaa
    10741 ccctgaatca atttacaatt gtttaaataa tagtttcttg gctctccttg aagaaaagta
    10801 ccgtatacga atagctttct gatttcacta atcatgtata ttgacaggaa tttctgtttt
    10861 tgaagatact accttgcctt ttactaaaac gagttttaca tcttctttac aacaggaaat
    10921 tactgaaaga tatggatcag gaaatggatt cttaggactt agaaaaatca aatcagcttc
    10981 ttttccttta tctatgcttc caatcctttc atctaagaaa agcgcccttg cacctccaag
    11041 tgtagctaaa taaatggtaa aaaatggggc tttttcccca agcttcttcc caaatgttag
    11101 ataaaagaat ctaagctctt caaaaaagtc caagttgaaa ttactagaga gcccatctgt
    11161 tccgattcct attctctcga gatctgctaa atactttagt ggaggtgcgc caaccttgag
    11221 aaagaaatta cttcttgggc agagaactat accaatatca tgtttattta tctcttccac
    11281 ctcttctggt aggatctgaa ccatatgaac cgctgtgact tttgtcccat taaaaaattt
    11341 taatcccttt agatattgaa acggggtatc agatttgtct cttacgaatg attctttgcc
    11401 tataagtgga aatatcttct tctcaaaacc gtttacttcc cctcttatga acttaacctc
    11461 atcagggctc tcggctagat ggatgcccaa tgggatacct ctttgatggt tgaacctcca
    11521 aacctttttt aggaattcag gagaacacga ataggttgca tgagggaaag gtctttcttc
    11581 aaaggtttca tccttatcga aggaaaaaat ctcccagaag tctccatgcc aatcaaatag
    11641 ctcgggaaag agaaccgttc tcatacctga ttttttaagt aaaggcttat ctaagccacc
    11701 ataggaagaa atttcaccta tagtggttac gccgcattgt aagagcattt tgatcccttc
    11761 ttcaacggat gtttctattt cttctcttga tgtgtctgcc atcctagctt ttataatatg
    11821 ttcaagccag cttatgaatc cactgaactt cccaatcctc ttgcctatcc atcctagctc
    11881 aaggtgggtg tgggcattta caaatccagg aagaaggatt ccatttccga gctcaagagt
    11941 aggtatacct tgatatttct ttttaagctt agatgctttt ccaatttctc taatctttcc
    12001 atcaactatc aaaatggaac cctttattag cggaggggag gaaattggca aaaagttttc
    12061 tgctgttatt agtaactttt ccatctagtg aatatttgac tgcaaatgga taggctccat
    12121 tagccaaact taaactgcaa gcacaaatta ccaaatcaca aattccaatt cccaagaatc
    12181 cagaaggata aaatctttct tctaagtact gacttctagc tcccgacttc ttatttgtga
    12241 ttttgtgctt gtgatttgga atttgtggtc ctattcatgg ttgagttatt ataatgcctt
    12301 tggttctaaa ccgaggagaa tttgttaatc ttttctaata agtcttgact gatcgtgccc
    12361 acgtaaactt cctccactgg tcctcgcatc tttattgacc agtcatccct gatttgaatc
    12421 tgtaactctc cgccaggcat tgatacggtt acatatttgt ctgtcaaacc atttctaaaa
    12481 caagctgcag caactgcaca tgaactgctt cctgacgcaa gggtgtatcc aactccgcgc
    12541 tcccagataa gaatatttac tttattcctt gattccacgt gagcaaactg aacgttaatc
    12601 ctattaggaa aaagtgggtg ggtttcgagt atcgggccca gtttcctggc atatccttca
    12661 tcaagttcgt taacaaaaaa cacacagtgt ggatttccaa ctgagactgc tgtaaatctc
    12721 agtatttcgc catttaccct gatttcttcc ccaaccactt ctctgtcctc cccttcaacc
    12781 ggtatgagct tgcttctaaa agtagcctcg cccatttcta ctatgacaaa agatactatg
    12841 ttgtttatca cctctagctc agctgtgact ataccaccag gtgtatcaat attgaacaac
    12901 tcttttttag tatattcatg ttcgtataaa aattttgcga aaatccttag cccatttccg
    12961 cttttctctg ccgaacttcc atctggatta aagatcttaa gcccaaagtc tgttttgttt
    13021 gaaggtacaa gaaggagtat cccatccgac ccaattccat agttcctatg acagataagc
    13081 ttgataacat caggattcag ttcaaatgaa atattggatc tatctagaat aatgtaatcg
    13141 tttcctagtc cgtgggattt tacgaattta ttcattggga tcttctaata ttgtatgtta
    13201 ttttctcgaa aaggtaagat gcaataggtt gtgagaaaat cctataatat tattaagaaa
    13261 attcaatttg gtggctagga ctgggaaact ttgaattggc ctatatatta gtacgacaat
    13321 cgcaaaattt tagtcttctt agtaataggg gttgcctgat aaataagaga ctgaaatcag
    13381 accacctctt cttgataacc ctctgctcca cggaagatta ccttgaataa atttgtagct
    13441 gagattatgc ctaccaattt tctcccatcc attactggta gataacgaac gttttcttca
    13501 tacatgatcc ttacaagctc atcaacgcta tcttcagcct gggcacattg ggcactctca
    13561 gtcataacat ctctaatctt tgtcttttca gggttttttc cttcagcaac aacccgaact
    13621 aaaaggtcat tttgcgtaaa tataccaaaa gtcggttttg gggtttgaat aataagcgca
    13681 cctatctcat gctttgccat gatttgtgct gcttccttta ctgacgtatt ctcatcaacg
    13741 gatataatgt cctcactcat gatttcacgt gctttcatga ttttaactca tcctttctta
    13801 atcaaaagta aaaaattgcc aaaggttgag atactcgatc tctttgagtg aaatttatga
    13861 tataaattat tcttctaaaa taaacgtaat cttaacgtgg gcgcgatatt cggtgatttt
    13921 gcctttttct atatgagcgt tcatcttggt aacctcgata ccggtgagcc cgcgaagagt
    13981 cttgtttgct ctcttgagag cattttggac tgcatcctcc catgatttgc tcgatgaccc
    14041 aatcacctct gatattcttg ctacagccat agtagtagtc tccttattat gttatttgtt
    14101 atcaacattt atatcaaggc ttgtaaagaa agtcaagtag gaaatatatc atttatgcta
    14161 aaattttata cacaaaacct ctatcatatt agatttgtga tagagatgct tggacagatt
    14221 ttatcaaacg gtggaaatag tagacttaat tagcagccca agttttggga tacccttgtt
    14281 agttaatatc tatgttgatc tctgatgtta tatcctgaat agttcttgtg tacttaagaa
    14341 gttctagctt aggataattc agtaaaattc acttagacct tgctattaac caatctgcta
    14401 cccttggcca gaggtctttt aaggccttgc tactaaccga gagccctatg tgtccagttg
    14461 ggtaaataac agtctcttta tctttacttg atattaggtc attgaagacc ttgctagagg
    14521 caggaggtac gagatggtcg tgctctgcta taaggtttag tgtagaacac ccaatctttt
    14581 taagctcgac aagctttcca ttgattctga gtttgttttc tataagtagg ttttcctgat
    14641 aacataattt aataaattcc ctaaaaacct cgccagctac tgggatatta tcgtttatcc
    14701 atttttccat agcaaggaag ttttcaacaa agtcttcatt atctatgttt tcataaaagc
    14761 ttacatattt agaaaccatg ttctgtatcg gtttcataat aagaaatcct gtctgtaaga
    14821 attctggagg aacattcccg tatgcatcaa tgaatttgtc aacatcgaaa
//
LOCUS       DATDRL010000148         9756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1632695, whole genome shotgun sequence.
ACCESSION   DATDRL010000148 DATDRL010000000
VERSION     DATDRL010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9756
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632695"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VI935_05450"
     CDS             complement(<1..652)
                     /locus_tag="VI935_05450"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HLE25078.1"
                     /translation="MRINILGCGGSKGNCSKPTAFLIDGKLLLDCGTATEVLSLKKFT
                     LIDNIIVSHAHIDHIGDLPFIANLAFDMNKDPIVLHGIEQAIKDISTHIFNWRIWPDF
                     PKIPNAKDAKLSYRTLKPQKKVAVGPYSVMAIPVNHTVPTVGYLIDDGETALAYTGDT
                     YNTDLFWEKIRGEKRLRAVIIESSFPNRLEEAARTTKHLTPSLLEKEVKKLRRDDVE"
     gene            complement(949..1272)
                     /gene="grxD"
                     /locus_tag="VI935_05455"
     CDS             complement(949..1272)
                     /gene="grxD"
                     /locus_tag="VI935_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017325000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HLE25079.1"
                     /translation="MTEDIMKEIQSQIDRHKILIYMKGTREMPQCGFSARTVMLFESY
                     GVPFETVDVLADPVLRESLKKFSNWPTFPQVYIKGKLVGGCDICLELHERGVLEQMAK
                     TALEG"
     gene            complement(1443..1694)
                     /locus_tag="VI935_05460"
     CDS             complement(1443..1694)
                     /locus_tag="VI935_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HLE25080.1"
                     /translation="MDAEEIKVMIENGMPESIVKVEGDGTHFQAIVVSYEFEGKGLLE
                     QHQMVYKAIGEAMKDRVHALSLKTYTPEQWEQVTGGGNS"
     gene            2453..2686
                     /locus_tag="VI935_05465"
     CDS             2453..2686
                     /locus_tag="VI935_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HLE25081.1"
                     /translation="MAASAYVLVSLSGPQTKVALNNVRKITGVKSAHIVAGPFDLIMH
                     VEGKNLEEIGELVISKVRKMAGVTDTVTCYVVA"
     gene            complement(2777..3007)
                     /locus_tag="VI935_05470"
     CDS             complement(2777..3007)
                     /locus_tag="VI935_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25082.1"
                     /translation="MDEILTLSDEEVDYWGDLYLKWQQSISEDMLNLLSDFERQQYLW
                     AKRSTFSQFVNLCLKYVCSSSQLNNQRKLKYG"
     gene            3141..3446
                     /locus_tag="VI935_05475"
     CDS             3141..3446
                     /locus_tag="VI935_05475"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLE25083.1"
                     /translation="MDKLKSVVECLKRYDPEEIMIFGSYARGDADEQSDLDLVVIKQT
                     KKRFLERLIEVAGFLDNDLGKVDVFVYTRKEFDEMKNKGNPFIEKVLKEGRVIYKAK"
     gene            3457..3849
                     /locus_tag="VI935_05480"
     CDS             3457..3849
                     /locus_tag="VI935_05480"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HLE25084.1"
                     /translation="MRWLRQAEHNFAVTKHNFTAGFYSDACFMAEQAAQVALKAFIIA
                     NKRRYIWEHSVQELARLCQQYDNDFAGLVEAGKILDRYYIPTRYPDALASPAVPYKTY
                     TKNDAVKAIGFAKSIIQLVKRKLKIRFK"
     gene            3852..5381
                     /locus_tag="VI935_05485"
     CDS             3852..5381
                     /locus_tag="VI935_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HLE25085.1"
                     /translation="MISKILSSAVFGVDAYLVEVEVDIAFGFPQFSTVGLPEGAVKES
                     KERVKAAVKNCGYDFPQKRITVNLAPADIKKEGSAFDLPIAIGILAATGVADTEKLGN
                     YIILGELSLDGRVKAVRGVLPITICVRDSGLKGIIIPKENADEAAVVNGVEVLAIESL
                     PEVVEFLSGIKEINPTSIDVNKVFNQSREYLIDFQEVKGQEHVKRSLEIAAAGGHNVL
                     MIGPPGTGKTMLARRLPTILPDMNFEEALETTKIYSVAGLLPINSALIATRPFRSPHH
                     TISDAGLIGGGAVPKPGEVSLAHNGVLFLDELPEFRKNVLEVMRQPLEDGSVTISRAA
                     TSIAYPSRFMLVAAMNPCPCGFFGDPHKECSCSPIQIQKYRTKISGPLLDRIDIHIEV
                     PTVKYRELSSETAGEPSSKIRERVNHAREIQKRRFHGGRIYSNAQMTPRYVKKFSKPD
                     ESGIKLLETAVDRLGLSARAYDRILKVARTIADLEESEEVRSHHIAEGIQYRSLDRNL
                     I"
     gene            6184..7164
                     /locus_tag="VI935_05490"
     CDS             6184..7164
                     /locus_tag="VI935_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HLE25086.1"
                     /translation="MKKRILITGGAGFIGSHLSERLLNDGHEAICVDNFFTGTKDNIK
                     HLLKNPDFEIVRHDICFPLYVEVDEIYNLACPASPIHYQFDPVQTTKVNVLGSINMLG
                     LAKRLKVRIFQASTSEVYGNPTVHPQPETYWGHVNTIGPRACYDEGKRCAETLFFDYY
                     RQHKMDIKVARIFNTYGTRMHPNDGRVVSNFIMQALHNKDITVYGDGSQTRSFCYVED
                     MVDGIIRMMNTPKGFTGPVNLGNPSEVSILELAKLIIDLTGSGSRILFKPLPQDDPER
                     RRPDTTLAQEKLGWKPTVSLEHGIRKTIEYFEGIIKAQEGKKAKVHFKVL"
     gene            8613..8954
                     /locus_tag="VI935_05495"
     CDS             8613..8954
                     /locus_tag="VI935_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25087.1"
                     /translation="MLCADDATDKASDRETNLLGNGVGAFIWLNSPPPGDTSLYCEVT
                     AQGDSKTDLVATYTLTFTHCLTGNTNYWWAAFTTPVPSGDPGVYVYTMECYTEVNGDN
                     IGKDTWRQILE"
     gene            complement(9481..9696)
                     /locus_tag="VI935_05500"
     CDS             complement(9481..9696)
                     /locus_tag="VI935_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25088.1"
                     /translation="MNVISAGSVNASFGWAQDARDKIDIVGDCLAGLVRQSGLENPDY
                     PFVCYPFGYRLDFFPDVFVEDQPFVPA"
BASE COUNT         2958 a         1927 c         2118 g         2753 t
ORIGIN      
        1 tctcaacatc atcacgcctg agctttttta cttccttttc taaaaggctt ggggtaaggt
       61 gtttcgttgt ccttgctgct tcctccaagc gattaggaaa agaggattca ataatcacag
      121 cccggagtct cttttcgcct cgaatcttct cccagaaaag gtctgtattg taggtgtctc
      181 cagtgtaggc taatgcagtt tcgccatcgt ctatcaagta ccctacggta ggcaccgtat
      241 ggtttacagg aatagccatt acactatatg gaccaactgc taccttcttt tgcggcttta
      301 gagtcctgta agaaagtttt gcatcctttg cgtttggaat cttagggaaa tccggccata
      361 tgcgccagtt gaatatgtga gttgagatat cttttatggc ttgttcaatt ccatgtaaaa
      421 caatagggtc tttattcata tcaaaagcta agttagctat aaatggaagg tcgcctatat
      481 ggtcaatatg cgcgtgtgat acaataatat tatcaatcaa cgtgaatttc tttaaactta
      541 aaacctccgt ggctgtgcca caatcgagaa ggagtttccc gtcaatcaaa aaagctgtag
      601 gtttagagca gttaccttta ctgccgccac agccaagtat attaattcgc atcttttacc
      661 cgtgtaattt aaactctgac atggatttaa acccatatag caatttgaac gcctttatat
      721 tatcagccgg gtagttcgat agcaagttat gcactacccg gcttaaatca caagactatt
      781 gcttatagcg gaatgtcaat cgaaagaatt tcattaatgg gacgtagtat aattcacggt
      841 tcctgtacgg ttttagatat tttcttccac tcttgtgtgt ttgaaagcta aatcaaatag
      901 atttgtttac caaaattagg acaaaattta attagtgaag gtataagatt atccctccaa
      961 agcagtctta gccatttgct ctagcacacc gcgttcgtgc aattcaagac agatgtcaca
     1021 gccgccaact agcttccctt ttatatacac ctgtggaaat gtaggccagt ttgagaattt
     1081 cttcagcgac tccctgagca ctgggtcagc aagcacgtct acggtttcaa aagggacacc
     1141 atatgactca aaaagcatta ctgttctcgc agaaaagcca cattggggca tctctctagt
     1201 tcctttcata tatataagta ttttatgtcg gtctatctgt gattgtattt ccttcattat
     1261 gtcttccgtc atgttttgct cctcatatct tgatactgat attttacttg gagagattat
     1321 aagcgcaatc tgtcaataga aattttaatg tagtaaaaca taaggtcacc agcaagactg
     1381 gtgaactaac cgatttttta ttttatatct tcatcaattt accgcgcagt cgtaggagca
     1441 attcatgaat tgccaccacc cgtgacttgt tcccattgct cgggtgtgta ggttttcaaa
     1501 gagagtgcat gaacacgatc tttcatggct tcacctatag ccttatacac catctggtgc
     1561 tgctcaagta gtccctttcc ctcaaactcg tatgagacca cgatagcttg aaaatgggta
     1621 ccgtctcctt ccaccttcac aatagattca ggcattccat tttcaatcat tactttgatt
     1681 tcttctgcgt ccataaaaac tattataccc tctgcaagtt gtcatttaaa taaatcagac
     1741 tatatcttca ctagaacctc taaaatgaag aaccctgaag caatcccccg tttaaccggg
     1801 ggacagggta tcagcacata agaggggttc atcgtttcaa tcttcatttg gagacctaaa
     1861 ggtctccgct acattttgaa tccatcacaa aacaaaaatt gtagcggaag gctttagcct
     1921 tccatgaaca atacccctgg tcattgcaat gacagcacgg aagtgtcatt gcgaggaacc
     1981 ctgagcgacc cccgatcaag tcgggggaag gggacgaagc aatctgcaat tagccatcag
     2041 ctatcagtaa tcagccatca ggaaaaacat tatagctgag agctgatgtc tgaaaactgt
     2101 ttagattgct tcgctgggat ctgtggtatc cccgatcgag tcggggatca gggggagggt
     2161 cgttagggaa ccacaacgct cgcaatgaca gacaagcata gaattgcttc acggagttta
     2221 cactgagttt tacgaagtgt tcgcaatgac ttttgaaaac ctctagcaag ttaaattgag
     2281 ttgtcgggtt aaattgatca ccttcagctc aatatgtttg ggaacctatc ccctcgctta
     2341 tttttatcct taggtctgag tttttagttg ctgttccggg tgatagtatt taccaaattt
     2401 aatatttgct atatcctaac ttaatgaaat ccaaaacgag gaggaagcat agatggcggc
     2461 tagcgcatat gttcttgtaa gcctgtctgg gccacagaca aaagttgcat taaataacgt
     2521 cagaaaaata acaggagtaa aatctgcgca catagttgca ggtccatttg acctcattat
     2581 gcatgtagaa ggaaaaaatc tagaagagat cggcgagctt gtaatatcaa aggtaagaaa
     2641 aatggccggt gtaactgata ccgttacttg ctacgtagta gcttaagtgt gtgtacactg
     2701 ataaacgcgc ttctaataag ctcctaaaaa gcaactagtg tacaacgaat gttaattttt
     2761 tatttaaaaa taaatcctaa ccgtacttta attttcgttg attattcagt tgagaggagc
     2821 tgcatacgta ttttaggcag aggtttacaa attgagaaaa cgtcgacctt ttagcccaaa
     2881 gatactgctg cctttcaaag tcagaaagaa ggttcagcat gtcttcagag attgactgct
     2941 gccatttaag atacaggtct ccccagtaat ctacctcctc atcagacaag gttagaattt
     3001 catccattct ttttggatac ctccttttat ttttttgatt cattaaatca taaatgtata
     3061 ggaatgacaa gggattttgt tgaaaatcgg ataaaaactt tcttgtcata ggattgggtt
     3121 tagtggtata ttgggtgaag atggataagc tcaaaagcgt agtcgaatgt ctaaagcggt
     3181 acgacccgga agagataatg atttttggct catacgcccg tggggatgcc gacgagcaga
     3241 gcgacttgga tctggtggtc atcaagcaaa ctaaaaaacg ctttcttgaa cggctaatag
     3301 aagtggcagg gtttcttgat aacgacctgg gtaaagtaga tgtgtttgtt tatactagga
     3361 aagagttcga tgagatgaaa aacaagggaa atccatttat tgaaaaagtc ctaaaagagg
     3421 ggagggttat ctataaggct aaataacgaa gaatctatca ggtggcttcg gcaggcggag
     3481 cataactttg cagtcactaa acacaatttt acagcaggtt tctactctga tgcctgtttc
     3541 atggcagaac aggcggccca ggttgcatta aaggctttca ttatcgctaa taaacgaaga
     3601 tatatctggg aacactcggt tcaggagctt gcacgacttt gtcaacagta cgacaatgat
     3661 tttgcaggat tagttgaggc gggtaagatt ttagatcgct attatattcc cacgcgctac
     3721 cctgatgccc tcgcaagccc tgcagtacca tataaaactt atactaaaaa cgacgccgtc
     3781 aaagccatag gttttgcaaa aagtataatt cagcttgtaa aacggaagtt gaagattagg
     3841 tttaaatagg catgatttca aagatactaa gtagcgcagt gtttggggtt gatgcctatc
     3901 tagttgaggt agaggtggat attgcctttg gctttccaca attctctacc gtagggcttc
     3961 ccgagggggc ggtaaaagaa agcaaagaaa gggtaaaagc ggctgtaaaa aactgcggtt
     4021 atgactttcc tcagaaaagg atcacagtaa atcttgcccc tgctgatata aaaaaagagg
     4081 gttccgcctt tgatttaccc attgcaattg gtatccttgc cgcaacaggg gttgcagata
     4141 ccgagaagct aggtaactac atcatacttg gagaactttc tcttgacggc agggtaaagg
     4201 ctgtaagagg cgttcttcct attacgattt gtgtgagaga ttcagggcta aagggaataa
     4261 taatcccaaa agaaaatgca gatgaagctg ccgtggtaaa tggagtggag gtactagcta
     4321 tagaatcgct gcccgaggtt gtggagtttc tctcaggcat taaagagata aatcctacca
     4381 gtattgatgt aaataaggtt tttaaccaaa gccgtgagta cctgattgac tttcaagagg
     4441 tgaaaggaca ggaacatgta aaacgctcct tagagatagc ggcagccgga gggcacaatg
     4501 ttctgatgat aggcccaccc ggaacgggaa agacaatgct cgcacgaagg ctccctacta
     4561 ttcttccaga tatgaatttt gaggaggctc ttgagacgac gaagatttat tcagtagccg
     4621 gactcctccc cataaactct gcccttattg ctacaagacc atttcgatca cctcaccata
     4681 cgataagcga tgccggactg attggaggcg gcgctgttcc aaagcctggt gaggtaagcc
     4741 ttgctcataa cggcgtttta tttcttgatg agcttccaga atttagaaaa aacgtccttg
     4801 aggttatgcg tcaacccctt gaagatggaa gtgtgacaat atccagggct gcaacctcaa
     4861 ttgcatatcc atcaagattt atgttagtag cagcaatgaa tccgtgtcct tgtggttttt
     4921 ttggggaccc gcataaagag tgttcatgct ccccgatcca gattcaaaaa tataggacta
     4981 agatttcagg gcctcttctt gacaggattg atattcatat agaggtccca acggttaaat
     5041 atagagaact ctcgagtgaa actgcggggg aaccgtcatc taaaataaga gagcgtgtaa
     5101 accatgcaag ggagattcaa aaaaggagat ttcatggagg gaggatctat tcaaacgccc
     5161 agatgactcc aaggtatgtg aagaagttct caaagcctga tgaatcagga ataaaacttc
     5221 ttgagactgc agtagatagg ttgggattaa gcgccagagc ctatgataga atccttaaag
     5281 tcgcccgtac aatagctgac cttgaagaat ccgaggaggt ccgctcacat cacattgcag
     5341 aaggaattca atataggtca ctagatagaa atttgattta gtggcaaggc cgtttagagt
     5401 gcgtcaatag ctttaacttg agaattccct gtagcttgct acagggtttc cacaatagtg
     5461 tattgtcatt gcgaacacat tcacttcatt cagtgtaaac tccgtgaagc aatcccgtat
     5521 ctagattgct tcgtcgcttt gctcctcgca atgacagcta taacggtata tttttaagac
     5581 cataccctgc agcttgctgc agggttattg attatgtttt atttgaacat tatacccgct
     5641 tgacattaag ggagattttg ttaatttaat ttagcttttt caaaaatacg tagtattgac
     5701 ccgtgtcata tgaggatgaa acataaagcc tgtaatcaaa attcgtagtt agttggatat
     5761 tcattctggt tagtaatctt aaaccacata aaaaggggct aacgacgaat atgggttatc
     5821 agggttctaa tacgattttg atttattatc tagcacaatt gcactcttaa gactttgttc
     5881 ctagaattaa taaccttaaa gattcatgta aaatgaaaag ccctcaaaag tgttggagcg
     5941 aaattttggt ttcgagataa tgtcttatga ggttataata aagtttaagt taatagatag
     6001 tataggttat acatatttct taattgtctt taaaaattat tagattatga aaatgtaact
     6061 gagaaggcgg agccttgaat ttatgcaagg agttaagtca aaaatggtta ataaaattct
     6121 ttaaaccgat tatgttaatc ttatctctcg cttaaattaa gaattgaaaa taggagtcaa
     6181 gacatgaaaa aaaggatttt gattactggc ggcgctggat ttatcggttc tcacctttct
     6241 gaaaggcttt tgaatgacgg gcatgaggca atctgcgttg ataacttttt cacaggaacg
     6301 aaagataaca tcaagcatct attgaaaaat ccggattttg agatcgttcg ccatgatata
     6361 tgttttcctc tttatgtaga agtagacgaa atctacaacc ttgcctgtcc cgcatccccc
     6421 atccactatc aattcgatcc tgtccaaaca acaaaggtta atgttttggg ttctataaac
     6481 atgcttggtt tggctaaaag gctgaaggta aggatttttc aggcatcaac tagcgaggtt
     6541 tacggaaacc caaccgttca cccccaaccc gaaacctact gggggcacgt aaacacaatt
     6601 ggcccacgag cctgttatga cgaaggcaaa agatgtgctg aaaccctttt ctttgactat
     6661 tatagacaac ataagatgga tattaaggtt gcaaggatct tcaataccta cggaacccgg
     6721 atgcatccaa atgacggccg tgtggttagc aattttataa tgcaggcttt acataataag
     6781 gacataaccg tttatggtga tggctctcag acaaggtcat tttgttatgt cgaggatatg
     6841 gtagatggca ttattcgcat gatgaacact cctaagggat ttaccgggcc tgtgaacctt
     6901 ggcaacccca gcgaagtaag catactagaa ctagccaaat tgataattga tctcacaggc
     6961 tctggttcta ggatattatt caagccgctt ccccaggacg atcctgagag aagaagaccc
     7021 gataccacac tggcacaaga aaaactcgga tggaagccaa ctgtttcgtt agagcatgga
     7081 attcgaaaaa cgatagagta ctttgaggga ataattaagg ctcaagaggg gaaaaaagca
     7141 aaggtgcatt ttaaagtgtt gtagttctta gaataaggaa agtaaatcat ttcaattgaa
     7201 aaacagtaaa cctatcacta agctcatatc ggaaaatcag caagaaatta agaataatac
     7261 agcaagctgc gagctatcat catttaatca aaaaattcat tcagttgcta cgagttgggc
     7321 aaggcgtact atttgtcatt gctaactaag tgaagcaatc cggtatttag attgcttccc
     7381 ccccgaagac ttaatcgggg gttgcaatga ctggatacct aacctgtcct gagccatgtc
     7441 gaaggaaggt ctccgctaca ttcagaatcc atcacaaaac acaaattgta gaggaaggct
     7501 ttagccttcc ataaacagta tctttagtca ttgcaatgac atcatggact aaaagtgtca
     7561 ttgcgaggaa tgaaacgacg aagcaatccc cagaatatta gtaacagatt gcttcgctac
     7621 cgctcgcaat gacgatcggg acttatagag agaaatgtag ggctcgttcc ccgaacgagc
     7681 ttttagggct gatgaacgca cccctttttc ctggtggatg gcaacgagca agcctcgttg
     7741 cctacccgtt atatgacaat ataaaaactg gggcccatca gtcataaaaa atggttagcc
     7801 taccgggatc tgtggtagct atccagaggg aggttctttt agggaaccac atgcttgctg
     7861 gtaggcgttc agccaacgag caagactcat tgcctaccca cggcgacttt cactcgcaga
     7921 caacacggaa atgtcgttgc gaggagcgaa gcgacgaagc aatcccaaaa tgagagattg
     7981 cttcacggag tttacactga atgaagtgaa tgtgttcgca atgacagtgg gacaaccttt
     8041 tagtaatcga tagggtatta tcaagttaaa cttatgattt tgttaaaagc cataaaaatg
     8101 aaatttatta aagaaagaca aatagttatg gactaagtat gggttctact tagctttaat
     8161 tgatccttat aaatgttaag gaaatatgaa aattcttgac ttttaaatta tatgggtgta
     8221 tttagtaata acatcttaaa attgataggg gttttcaatc tttaagagga tggaatctta
     8281 cagaccagtt tgccgatttt actttgtcat tgccagagaa ttataaccat aaaagtcaat
     8341 tgaactggct tgttaggttt aattctagaa agaaaggagg tgatgaaaag gaaaataaaa
     8401 ttgtagactt gttcgatgac taaatagtgg aaccggttat cgaaaagcct taacttcaaa
     8461 aaaggttcca aaaaatctaa aatcttatag gaggtttaaa aatgtttaaa tttcgattct
     8521 ttagtttagt tgttgtttta gttatgttgg tactgggttt atctgctgct ccggtattcg
     8581 cagacaccgg cggtattaag gtggacgatg atatgttgtg tgcggatgat gcaaccgaca
     8641 aggcgagtga cagggagacg aacctacttg gcaatggtgt aggggcattt atatggttaa
     8701 actcaccgcc tccaggtgat acctcgttat attgtgaagt cacggcgcag ggcgatagca
     8761 agactgacct tgtagctacc tacactttaa ccttcaccca ttgcttgacc ggcaacacta
     8821 attactggtg ggctgctttt actacaccag taccatcagg agatccagga gtatacgttt
     8881 acacgatgga atgttatact gaggtgaatg gtgataatat aggtaaagat acctggagac
     8941 agatactaga ataagacatg ggaaaaaaga ggaaaaaagg ggacgcaccc ctttttaagg
     9001 ctattgtcat tgcgagttaa gcgaagcaat ctcatcattt gggattgctt cgtcgcggaa
     9061 ttaatcctaa gctgggacga agggctgatc cccgacaaag acatcgggga taaagtccag
     9121 cctatcccca tatggataac aaacaaaggg ataggcgggg ttttctaacc ccgactgtct
     9181 aaccccgcta agcaattccc aacaatgtca attttatccc gtgcatcctg agcccagtcg
     9241 aaggatgcgt tcaccgaacc cgccgaaata acgttcattt ggggtactga aatctccctt
     9301 aatccctctt ttccaaagag ggagactcac ctcgttcgca tgcgaataag gattatgggt
     9361 cagagcttaa ccttataccg tttttggaaa aaggagacgc accccttttt aaggctattg
     9421 tcattgcgag ctaagcgaag caatctcatc atttgggatt gcttcgtcgc tgaattaatc
     9481 ctaagctggg acgaagggct ggtcctcgac aaagacatcg gggaaaaagt ccagcctata
     9541 cccaaatgga taacaaacaa agggatagtc ggggttttct aaccccgact gcctaaccaa
     9601 ccccgctaag caatccccaa caatgtcaat tttatcccgt gcatcctgag cccagccgaa
     9661 ggatgcgttc accgaaccag ccgaaataac gttcatttgg gatactgaaa tctcccttaa
     9721 tccctctttt ccaaagaggg agactcactt cgttgg
//
LOCUS       DATDRL010000149         6778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_429966, whole genome shotgun sequence.
ACCESSION   DATDRL010000149 DATDRL010000000
VERSION     DATDRL010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429966"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(380..2215)
                     /gene="glmS"
                     /locus_tag="VI935_05505"
     CDS             complement(380..2215)
                     /gene="glmS"
                     /locus_tag="VI935_05505"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HLE25089.1"
                     /translation="MCGIVGYIGKRNAVPILIEGLRRLEYRGYDSTGIAFYENGNIVL
                     RKTAGKLANLESIIRDHNYESHLGIGHTRWATHGKPTDINAHPHSDCSGKVMVVHNGI
                     IENYLSLKKTLIAEGHVFRSETDTEVIAHLIERYLHDGNLLEAVRKALHKVKGTYALA
                     VISNADPDKIIAARKGSPLIIGWGKEEYLLASDVPAILNYTKDAIFLNDNEVVVLHKN
                     GVEITDLENGYFRPERIYQIPWNPELAEKNGYPHFMLKEIHEQPQAIRNTCSGRINLD
                     KGKVCWDEVDLSREEIRKISTIYMVSCGTSWHASLVGKFMIEALAQIPVEVDLGSEFR
                     YRTPIIKGATLTIGVTQSGETADTLGAMRRAKESGSRVLVICNVVGSSATREAGGVIY
                     THAGLEIGVASTKSFTSQIVAFYLFALYLGRIKGLISRPLFCEMMTELAAIPGKLEEV
                     LRKEKKIEQFANLYWEKKNFVFLGRGITYPIALEGALKLKEISYIQAEGYAGGEMKHG
                     PIALVDKDMVVVSLIPRNFIYEKMLGNIEEVKARDGIVIAIANQGDEEIREKADHVFY
                     VPKSNDFLTPILFTIPLQLLAYHIARKRGFDIDQPRNLATSVTVE"
     gene            complement(2404..2925)
                     /locus_tag="VI935_05510"
     CDS             complement(2404..2925)
                     /locus_tag="VI935_05510"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HLE25090.1"
                     /translation="MSDCNSLHSRTDKEWYAVYTIVRHEKTVNSTLTYQNIETFLPLS
                     EVLSQWNDRRKTVQLPLFPGYLFVNIFLQDRWNVLNTCGVVRILGVNGTPIPVPVEQI
                     EAVKRLLESNLKYDPYPYFTQGTEVVVINGPLQGMVGRISERRGKYRLILSVDLIQRS
                     VSVELDIRDVELV"
     gene            complement(3189..3971)
                     /locus_tag="VI935_05515"
     CDS             complement(3189..3971)
                     /locus_tag="VI935_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLE25091.1"
                     /translation="MNQPIRVFIVDNNLLFTEAVAAILDAREEIVLVGLAANLDGMLE
                     KAPSDAIDVVLINTSIEQMDTAQLIQNIKEELPDVKVIVVGLSYREDDNLKFIEAGAS
                     GYVFKEGSIADLLRTIELVHCGQTPCSPKMAALVFTRIAELSQELGQGEMLQQAELTR
                     REKEILQFIVKGFSNKKIAHHLHIKLYTVKNHVHNILEKLQVHRRQGAIRYAHENGMF
                     KGFQFLRLFLGNTSSSKLGQGKAEGLEKVNPVISTFLNRKIY"
     gene            complement(4114..4485)
                     /locus_tag="VI935_05520"
     CDS             complement(4114..4485)
                     /locus_tag="VI935_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25092.1"
                     /translation="MTKIKVLLANRPQSLRKSLRNIIYSQTDMEVVGEALEPVEILLM
                     VGKTEADVVIVTLPNSDEDPGIYSHLFAEYPQLLIFAVSPELESALLYRQLISKEQIF
                     KVSKEEIPALIRKVKDKDMDR"
     gene            complement(4923..5648)
                     /locus_tag="VI935_05525"
     CDS             complement(4923..5648)
                     /locus_tag="VI935_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLE25093.1"
                     /translation="MKKRVEYGNIRMDGRMKSQNISLMIVDENKVFCECLASVITQKG
                     FTVIEQFMELREVLNKIKAQQPDIVLININLPNNMAIELIKHISLELSQTKVIILGLS
                     ETESRLTILECIEAGAKGYISKETSLNDLITIIRLVYQGETFCSPQIAYSIFSRVAEL
                     ARKRHDRLIPEFGNVTLREIEILQLVSEGLSNKQIAQRLSLSLYTVKNHVHNVLEKLQ
                     ARNRLEAVNYALDKGLLRKTRLR"
     gene            complement(6213..6470)
                     /locus_tag="VI935_05530"
     CDS             complement(6213..6470)
                     /locus_tag="VI935_05530"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HLE25094.1"
                     /translation="MSQYFTHPSCPKDKPFVERVIGTIEREFIQQGKLACDVKTQQKL
                     INEWLDEYHNYRPHQALGYLTPNEYYKKTIGKTIKQLLPMY"
BASE COUNT         1887 a         1544 c         1218 g         2129 t
ORIGIN      
        1 caccgagttt atactgagca cttcgcttcc ctcagtacag gctccgcgaa tgtgttcctc
       61 gcaatgacgc ctcttgagtc attgcgagct tagcgaagca atctcgtata gttcaaccaa
      121 ccgtagtaca tgcaagtgta agacgctata acgaaacaga aagtctcaac tgccaaaccc
      181 gcacaggtca atgagaccat gggactgtag accgccgttc aacccatccc cccggttgat
      241 tttcgtcagg tatgcatcgg aagcggaatt gatgaaataa aaatcgggtg ctccggttga
      301 cttggaggga gcggttgttt tgaagtccca ttgtcctatt gtcccgcaaa acttcctttc
      361 tatctaagaa attaccgaat tattcaaccg taacgcttgt agcaagattc ctcggttgat
      421 ctatgtcaaa tcctcttttc ctagcaatat gataagcaag aagctgaaga ggaatagtaa
      481 atagaatagg agttaaaaag tcgttactct taggtacata aaaaacatgg tcagcttttt
      541 ctcttatttc ctcgtcacct tgatttgcaa tggctatgac tattccatcc ctagctttta
      601 cctcctctat attgcctagc atcttttcat agatgaagtt acgaggaata agagaaacta
      661 caaccatatc tttatctaca agtgctatag gaccatgttt catctcccct cccgcatacc
      721 cctcggcctg aatgtaggat atttccttta acttcagcgc gccctctaaa gcaattggat
      781 atgtgatacc tcttcctaga aagacaaaat tctttttttc ccaatacaag tttgcaaatt
      841 gttcaatctt cttttccttt cgaagcactt cctcaagctt tccgggaatc gcagctagtt
      901 cagtcatcat ttcacagaaa agaggacgcg atattaagcc ctttatcctt ccaagataaa
      961 gggcaaatag ataaaaggca actatttgtg acgtaaaaga ttttgtagaa gccaccccta
     1021 tttcgagtcc agcatgagtg taaattactc cacccgcttc tctagtggcg ctgcttccta
     1081 caacattaca aatcaccaac acccgagagc cgctttcttt agctcttctc atcgctccaa
     1141 gggtatcggc tgtctcaccg gattgagtta cccctatcgt taaagtggcc ccttttataa
     1201 taggtgttcg gtatctgaac tccgatccta aatctacttc cactggaatc tgagccagtg
     1261 cctctatcat aaactttcca acaagcgagg catgccaaga tgttccgcat gaaaccatgt
     1321 aaatcgtcga tatttttcta atctcttcac gagatagatc aacttcgtcc cagcacacct
     1381 ttcccttatc caggtttatt cttccgctac aagtgttcct tatagcctga ggctgctcat
     1441 ggatttcttt taacataaaa tgtgggtagc cattcttctc tgcaagttca gggttccagg
     1501 gaatttgata tatcctttcg ggtctaaagt aaccgttttc aagatcagtt atttccactc
     1561 catttttgtg gagcaccact acttcgttat cattaagaaa aatagcatct ttcgtgtagt
     1621 ttaatatagc aggcacatct gatgctagaa ggtattcctc ttttccccac cctatgataa
     1681 gaggactacc ctttcttgca gcaataatct tatcaggatc agcattgctt attaccgcaa
     1741 gcgcataagt tccctttacc ttgtgtagcg cttttcttac ggcttctaat aggtttccat
     1801 catgaagata tctctcaata agatgtgcaa taacctctgt atcggtttcg gaacgaaaaa
     1861 cgtggccctc agcgatcagt gtttttttaa gcgagagata attttcaatg attccattgt
     1921 gcacaaccat taccttacct gaacagtcgc tgtgtggatg tgcgtttata tcagtcggtt
     1981 ttccatgcgt agcccagcgt gtatgaccaa ttcccagatg gctctcatag ttgtgatctc
     2041 taataataga ctcaagattt gctaatttac ccgccgtttt tcttaaaact atattcccat
     2101 tttcataaaa cgctattcct gttgaatcgt aaccgcgata ttcaagtctt ctaagccctt
     2161 ctatgagtat aggtacagcg tttcgctttc ctatataacc tactattcca cacattcgca
     2221 cttacctcca aaaaccacca atctatccaa cgaagtatta tctcttcacc actcctcttt
     2281 tacttcctgt accatgacgc tatatacagc ttcgccacct cactcacatg aaattgcagg
     2341 tagtgggtcg agcaattcga caatctcttc aaataaaatt gacgactata gacagcagag
     2401 tgcctaaact aactccacgt ctcttatatc caattcaact gaaacagagc gctgtataag
     2461 atcgaccgag agaataagcc tgtacttacc tcttctttct gatatccttc ctaccattcc
     2521 ctgaaggggt ccgtttataa ctactacttc tgttccttga gtaaaataag gataagggtc
     2581 gtacttgagg ttgctctcta aaagtctctt tacagcttca atctgctcga ctggaactgg
     2641 aataggtgtt ccatttacac caagaattct aacaacacca catgtattta aaacattcca
     2701 cctatcttgg agaaagatat taacaaacaa gtatcctgga aaaagaggaa gttgaaccgt
     2761 ttttcttctg tcattccatt gacttaatac ttcgctaagt ggaagaaacg tctctatatt
     2821 ttggtaagta agagtcgaat ttacagtctt ttcgtgtctg actattgtat agacagcata
     2881 ccattctttg tctgtacgac tatggagaga attacaatca ctcatcttct aacctcgtac
     2941 cttcaatctt gtaattgatc tttttaatcc tccacttatt tttctgccct gatagactcc
     3001 agcttattgt tctatagcag cgatttttgc gtccatgaca tgggtaactc atctgcaata
     3061 aaggctttca tgatttttct caaccattat cgaagctcac tacagtatag aatctcaact
     3121 cttgtctctg aaacaaaatc gctctcataa tcacttcctg tagactatca accaagccca
     3181 ttctggaatc aatagatctt ccggtttaaa aaagtactaa taaccggatt cactttttct
     3241 aggccctctg ccttgccttg tcctaacttt gaagaactgg tattgccgag gaacagtcta
     3301 aggaactgaa atcctttaaa catcccattc tcatgagcat accttatcgc cccttgccga
     3361 cgatgtacct gaagcttctc taaaatgttg tgtacatgat ttttcacagt ataaagttta
     3421 atgtggaggt ggtgggcaat cttcttatta ctgaatcctt ttacaataaa ctgcaagatc
     3481 tctttctcgc gcctggttaa ctctgcttgc tggagcattt ctccttggcc tagctcctgg
     3541 gataactcag caatcctggt gaaaaccaaa gcggccatct ttggggagca aggggtctgt
     3601 ccacaatgaa cgagttcgat cgtccgaagc agatcagcga tggatccttc cttaaagaca
     3661 tagccactcg ccccagcttc aatgaatttc agattatcgt cttccctgta gctcaatcct
     3721 accacgatca cttttacatc gggaagttct tctttgatat tctggatcaa ttgtgctgta
     3781 tccatctgtt caatgcttgt attaatcaac acaacatcga tcgcgtccga tggagctttc
     3841 tctaacatac cgtcgagatt ggctgccaaa ccaacgagga caatctcttc ccgagcgtcc
     3901 agaatggcgg caacggcttc agtaaagagc agattattat caacgatgaa cacacgaatc
     3961 ggttggttca ttaacgtcct ccttctcagc aaagaattca agccctcatg tcattttgag
     4021 cccctgagca gtggttctca gagtgccaaa agaaaaccac cgttgataga tttacaaaga
     4081 tcatataact tgagttgaac atctgccaaa gtttcagcgg tccatatctt tatcttttac
     4141 cttacgaatt agtgctggga tttcttcttt agataccttg aaaatttgtt ctttagaaat
     4201 gagttgtcga tacaagaggg cgctctcgag ttcaggcgat acagcgaaga tcaggagctg
     4261 ggggtattca gcaaacaggt gactgtaaat accaggatcc tcatcagagt tcggcaatgt
     4321 tacaatcact acatcggcct cagttttacc gaccattaag aggatctcga ctggttcaag
     4381 cgcttctccc accacttcca tatcagtctg gctgtagatt atatttctca acgatttacg
     4441 gagcgattgg ggcctatttg ctaacaatac tttaatcttc gtcatacatt tttaccccaa
     4501 tcttggaact ttcaactgcg accttcatta tcttccatta ccaccagtga agcaatagac
     4561 cgaaagattc aaaatgggtc taaaacgtcg attcatcttt tctagatcta gaaccaaacg
     4621 taagtgggga aacggagtgg agaggagatt ttaatcgtgg ttttgaaggg gaatatggat
     4681 aatcgggata aggtagaaag tctttctggt ttttcttaga ttaagtgccg tttctagaaa
     4741 aagatggttc tactttttct agaaaataac ggttctatgg atcaagcggt gaacgaatat
     4801 gaacaccact taggtcactt gatgcaaccg ttaatttttg gaaaaagtgc gaccgttttt
     4861 agattgaaac aacaattaag aaacaaatgg aggaaatatg tttaatttag aaataatatt
     4921 agctagcgta ggcgtgtttt tctcaataag cctttgtcca aagcgtaatt gaccgcctcc
     4981 aaccgattac gggcttgcag cttttccaag acgttatgca cgtggttctt aacagtataa
     5041 agagacagag acagccgctg ggctatttgc ttattactca acccctcgga gactaactgc
     5101 aaaatctcta tctcgcgcag cgtcacgttc ccgaactcag gtatcagtct atcatggcgt
     5161 ttgcgagcta gttcagcaac tcgtgagaat atggaatagg caatctgcgg agaacaaaat
     5221 gtctccccct gatacactaa acggatgatt gttatgagat cgtttaacga tgtttccttt
     5281 gaaatatacc ctttggctcc ggcttcaatg cactctagaa tggttaatct tgattccgtt
     5341 tcactcaatc caagaattat caccttcgtt tgtgacagct ctaaactaat gtgcttgatc
     5401 aactcaatgg ccatattgtt agggagatta atattaatca gtacaatatc cggttgttgg
     5461 gcttttatct tgttaagtac ctcccttagt tccatgaatt gctcaatcac ggtgaagccc
     5521 ttttgggtta tcactgacgc tagacattca caaaacactt tgttctcatc tacaatcatt
     5581 aagctgatat tctggctctt catccgccca tccatcctta tattaccata ctctactctc
     5641 ttcttcatat taccatactc tcttcttcgt cgcctataac cttttacttt ttcgtttgtc
     5701 tatccttttt gaagttcctg ttccctcaac tgttacctct tttttgaaat atttacttcc
     5761 ctagtgcttg ttcaggcaag gtcttcggga atgccgggcg tatagcagta tggctagagt
     5821 tactttttta ggagatatcc aagaggacat atggtgattt attcccgaaa aggctctgat
     5881 catgcagtag gagcaaaact gaatctcact tgctctatca gcgaaattaa gataggaatt
     5941 gaaatatagt tggtaagtag aaatacatct cttacctaat attggaatgt gtaaaaaacg
     6001 gttagaaaaa tcagttttaa gggaaaccac ccttactgta ggaagcaaac aataatattt
     6061 ggactgaata cgagttatca caacttaaac accttcgttc tatattcatt aacctcagtg
     6121 taaataatga aaaagctaac gcttcaaccc tatctcatga ttacatctcc attatacaaa
     6181 aattttagca agatttctag tcaagtcatg gttcagtaca taggtaacag ttgtttaatt
     6241 gtttttccta ttgtcttttt ataatactca ttaggcgtta aatatccaag agcctgatga
     6301 ggtctgtagt tgtgatactc atcaagccat tcatttatga gcttctgttg agtttttaca
     6361 tcacaagcaa gtttcccttg ttgaataaat tctctctcga ttgtcccaat cactcgttct
     6421 acaaatggtt tgtcctttgg acagctggga tgagtgaagt actgtgatat ctctgcctcc
     6481 tctaacggct cttggaaatc ctttaagttc tctccaccat tatcatttct ctaggattta
     6541 atcgaaaatg ggagtgtttg tttgatatat tcaattatta aggagttttg ccctgatgaa
     6601 gaggttgagg atatcttcac tgctatcgcc ttcccaacgc aatcaattgc aacaaaacaa
     6661 taaaacaata acgcttctgc cctagaaaag gcatatgttt cgtatccgat tctattagat
     6721 cacctggagc tttaatggga tagtctttag acagcttctt acggttgaca ctggcata
//
LOCUS       DATDRL010000150         2826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3478596, whole genome shotgun sequence.
ACCESSION   DATDRL010000150 DATDRL010000000
VERSION     DATDRL010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3478596"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            511..990
                     /locus_tag="VI935_05535"
     CDS             511..990
                     /locus_tag="VI935_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25095.1"
                     /translation="MKKVQMEAPDGRSELVQNPLGFDDLHLQRDGMTYADLIKKFPEG
                     KYSIKFSPSKFRGISFNLTYDFPSTPVITYPKDGATEVPLSFTITWESVSDIDGLQLN
                     IEDLLSVDLAVGSTSFSVPGGLLQQNTWYSMELIARKSSNENEMLSKRPIDFTTGSE"
     gene            1352..1558
                     /locus_tag="VI935_05540"
     CDS             1352..1558
                     /locus_tag="VI935_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25096.1"
                     /translation="MSIAKKQAIKLIKSLTDDCTIEDIQYHLYVLEKVERGIRAIDEG
                     RVVPQEEVEKRVRKWLKSSGQNQR"
     gene            1525..1842
                     /locus_tag="VI935_05545"
     CDS             1525..1842
                     /locus_tag="VI935_05545"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLE25097.1"
                     /translation="MAEVIWTEPALIDLHDIFEHIARDSLIYAEKFVNRVVVAVKRLE
                     LFPCCGRIVPEFNNESIRELIYEVYRIIYVVRKNECYIATVIHGSRDIFRHLEPGTWE
                     IMQ"
     gene            2020..2700
                     /locus_tag="VI935_05550"
     CDS             2020..2700
                     /locus_tag="VI935_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25098.1"
                     /translation="MFSRKSTIIATTFIAFMYLVFVPIVYAAKEYKMTDYWPFKEGAW
                     WEFNDGKIEITGTEEINGINTFKSLETGSCSENEEIERGGEFNYFNDDANGFGSFSQN
                     EDGSYLMVKISESYVKKGAKTKASFKVNDGTQVTVITKLEGTATVIVPAGKFKRCLRF
                     SIKVKGVHTDGSYSYWTEKFYLAKGVGLVKAVRIKQSGNDAGCGFGPCCLDVFNGIRE
                     LKNYSLAD"
BASE COUNT          914 a          435 c          673 g          804 t
ORIGIN      
        1 tgtatttgta ttctagtatt tgccgttcta ggcagatagg gattgacaat aaataacatg
       61 aacaccttca cgcctgcctg agcgagtccg cctgcctgtg tgtccgtacg cagacaggct
      121 cgcagacagg ttgctgtgcc atcggggcta aagccccggt atgacgtgta cccactaacc
      181 ccagccttaa ggctggggtt agtgacggtg ttatctgaat cgggctcttg tcctcgtgaa
      241 aacggggttt agccctgata gcgcctgatt ttcaaagtgt cggacaagta cagattattt
      301 attgtcaatt ccataggctg agagtaggat gggttaaacg aagcgaaccc atcgattatc
      361 tatcagaact tttatagaag gggaaatgcg aagcagaaaa gtgattcagg ttctaatatt
      421 gcggctaatg tgtcggtaga gaaatctgag ggaaaaaacg aggtagaata ctatgttgag
      481 tttgaaattt ctggggaggg attgaggaaa gtgaaaaaag tccaaatgga ggctcctgac
      541 ggcagaagcg aattggtaca aaatccatta ggttttgatg atttgcatct tcaaagagat
      601 ggtatgacct atgcagacct tataaagaaa tttccggaag gaaaatacag tattaagttt
      661 tccccaagta agtttagagg tatatcgttt aatttaacct atgatttccc ttctactccg
      721 gtaattacct atcctaaaga cggtgctacc gaggtaccac taagctttac gatcacctgg
      781 gaatcggtga gtgatattga tggtctgcag ctaaacatag aagatttact ctctgttgat
      841 ttggcggtag gcagtacctc tttttcagtc ccaggtggtc tgcttcagca gaacacttgg
      901 tatagtatgg agttaattgc tcgtaaaagt tctaacgaaa acgaaatgct cagtaagcga
      961 cccatagatt tcactacagg atcagaataa tacaaaatga tcatggttaa attacaaaca
     1021 cttggagtta ttaagataca gcaagagtag gatgggttga aacgacagcg cacccatcaa
     1081 aattgtttta aacaaattaa ggtttgtgat gggttcgctt cgcttaatcc atcatacaag
     1141 cactcaaaac aaagctatcc tttttaggcc tgagaggccg ttagggtata gcagagggtg
     1201 aagccctctg ataagaaaga gaaaaatatc aagccctgaa agggcgtaag aaatggtagg
     1261 gtggattaag cagagcgaat ctataaggtg taattgtcgt caggaataac aaagatatgt
     1321 tcgatgtgct cagcacagga ggaaagtaaa aatgtctatt gctaagaaac aagccattaa
     1381 attgattaaa tcgcttacgg atgactgtac aattgaagat attcagtatc atctttatgt
     1441 gctggaaaag gtagaacgtg gtattcgtgc cattgatgaa gggcgtgttg taccacagga
     1501 agaggtcgag aaaagggtga gaaaatggct gaagtcatct ggacagaacc agcgctgatt
     1561 gacctgcatg atattttcga acatattgca agagactctt taatatatgc agaaaaattt
     1621 gttaatcgtg tagttgttgc agtgaaaagg ctcgaactat ttccctgttg tggacgtata
     1681 gttccagaat ttaataatga aagcatccgt gaattaattt atgaagttta ccgaattatt
     1741 tacgtggttc ggaaaaacga atgttacata gcaaccgtca ttcatgggag cagggatata
     1801 ttccgtcatt tagaaccagg aacttgggaa ataatgcagt aagtttagtg gtttgtgatg
     1861 ggttcgcttc gcttaaccca tctgcaagcg taaagacaaa gaattcagga tacacccaaa
     1921 tgttatagac agtgctatag attcgttagg aagaaataaa gggcgattta tatagattag
     1981 attaaggcac tatcaaaact tttctggagg ggataaaaga tgtttagtag aaaatctacg
     2041 ataattgcaa cgacatttat agcgtttatg taccttgttt ttgtacccat tgtttatgct
     2101 gctaaagagt ataaaatgac agattattgg ccattcaaag agggcgcatg gtgggaattt
     2161 aatgatggta aaattgagat aaccggcaca gaggaaatta atggcataaa tacatttaaa
     2221 agtctggaga ctggttcgtg ttctgaaaat gaggaaattg agagaggtgg tgaattcaat
     2281 tattttaatg atgatgccaa tgggtttggt tctttttccc aaaatgaaga tggaagttat
     2341 cttatggtaa aaatcagtga gtcatacgta aaaaaagggg ccaaaacgaa agcttctttt
     2401 aaagtgaatg acggtactca agtaactgtg attaccaaac ttgaaggtac tgctactgtt
     2461 atagtgcctg ctggcaagtt taaaaggtgt ctaagatttt ctataaaggt taaaggagta
     2521 cacacagatg gaagttattc ttattggaca gaaaaatttt atttagcaaa aggggttgga
     2581 ttggtaaaag ccgtccgcat taaacaaagt ggaaatgatg ccggttgtgg ttttgggcca
     2641 tgttgtcttg atgtttttaa tgggataagg gaattgaaaa attattcttt ggcggattag
     2701 tggttttgcc aaaagaattt cctctaataa aattccaagc aacaaaaaca gatgtgttta
     2761 attgagctat aaaagagtaa agattattag agccttaaaa gaatgtggat ttgagttcct
     2821 gaaaga
//
LOCUS       DATDRL010000151         5054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_566987, whole genome shotgun sequence.
ACCESSION   DATDRL010000151 DATDRL010000000
VERSION     DATDRL010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566987"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..1863)
                     /gene="nadB"
                     /locus_tag="VI935_05555"
     CDS             complement(262..1863)
                     /gene="nadB"
                     /locus_tag="VI935_05555"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HLE25099.1"
                     /translation="MDDIHSKNINYLHSDFLVIGSGLAGLYAALYASRFGKVAILTKS
                     TLEESNSYWAQGGIAAAMDPEDSPLFHLDDTIKAGRGLCNREAVEILVREGVERVTDL
                     IALGMKFDLGEKGLELGLEGGHSRRRVLHAGGNSTGKEMVQFLISSVQANPNIFIFEN
                     TEVAELISNGERCFGALAFDLNIQKHLVLKAESTILATGGASGIYLRTTNPQTATGDG
                     IALGYRAGAEITDMEFIQFHPTAFYTENGETFLISEAVRGEGAYLLNNSLHRFMPEYH
                     ELAELAPRDVVSRAIFNEMKVSGKNYVYLSLKHLNPDFIRKRFSNIYNASLQYGLDIT
                     SDLIPVAPAAHYLIGGVKTGLWAETNIKGLFACGEVACTGVHGANRLASNSLLECIVF
                     ARRAVDLARESDTLTDKSDNLVKDLSFILNPDLQRDSFIKLKSIISQIMSEHVGIVRS
                     AYGLKRALEVFEKVASSGDELVGYNKLKLQNILDVCSLVARSALLRTESRGAHIREDF
                     PNEDPSWEAHIVLKRDSEPLVVRYN"
     gene            complement(1897..4551)
                     /gene="glnD"
                     /locus_tag="VI935_05560"
     CDS             complement(1897..4551)
                     /gene="glnD"
                     /locus_tag="VI935_05560"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HLE25100.1"
                     /translation="MWIKERKEMQRAGKLLARDGLKDRIRLYLEENRKKLRELHDKEA
                     LSGRQLAERRSRVIDELISKAITGLGFSKLSGVSIVALGGYGRDELCPYSDVDLLFLF
                     APRNESLAKEAVQALLYLLWDLNLEIGHSVRTIDDCMEISRGDDATIFTSLLDGRFIF
                     GDKKLFDQLDRIMYQELLPEISSKFIEGKIAENEKRIERFGRSVYLIEPHVKEGEGGL
                     RDIHSALWVAKAKFKVRSFRELLKKGVLLERELRVFEKGLDFLLPIRTELHYLAGRRE
                     DRLSFEWQEKVARFLGFRDVGELAAVERFMRIYYLRANLTREYSKKLVERSVSKPKVS
                     FQTPKTVFLDNSFIIQGGMLSVSKRTIFQEHPENLIRAFEVADRYGIKMSKYLHDLIR
                     DNVKIIDDNVRRNPGLNASFIWLLKEGKEVANALFDMNRLRLLGHLIPEFGKIVCMVQ
                     HDAYHVYTVDIHSIFMVNEIENLKKGEYEKQFPLVTQIAKEVSKRHILYLACLFHDMG
                     KGEGRDHSQRGAGMIPRISERMGLTEEESDQLEFIVKHHLVMPHFSQRRDIHDDGLIL
                     RFARSVKTLETLSMLYLLSFADIRSVGPDVWTNWKGMLLEQLYKGTANVLARGEFKRE
                     TPKRKSRRFIREVIQLLKNEIPESNIRRHLDVMPYTYFLGFSPEKIVYHVKLFEGFGD
                     DTGCDVIYNENEGYDEFTFWGYDKPGVFSQLCGVLAGSSINILGARIITRDDGRIVDV
                     FYVNRLGKSTYEDKEIWNKVKSNLHGVLKGEIDVEQLVSRRKRQKPIYIKPIPQHPTR
                     IDIDNESSDTATVMDIYTNDRVGLLYDITKTLTKLGLSIDYAKISTKVDQVADVFYVR
                     EIEGGKVFDKKNLENIENKLIEAIDIVA"
     gene            complement(4659..>5054)
                     /locus_tag="VI935_05565"
     CDS             complement(4659..>5054)
                     /locus_tag="VI935_05565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLE25101.1"
                     /translation="DEGLVVFAELSQKNASLAKEFFRRGGLDNRVEIHVGDALQILEE
                     TDGQFDIIFNDIDKEYYPLVVDEAYKKLRRGGLFITDNVLWFGRVLTDDLSPSTKGVK
                     EFTRLLFEKKGFLTTIIPIRDGISLSVKL"
BASE COUNT         1498 a         1189 c          833 g         1534 t
ORIGIN      
        1 tggggcattc tgaggggtca aatttttacc atccataatt gtcggacagc ctgtaagctg
       61 cagggtatca tcatataaac gaacagccct tctgtcattg cgaggaaccc tgagcgaagt
      121 cgaaggggac gaagcaatcc ctaaatagga gattgcttcc ccccgaagaa ttcatcgggg
      181 gtcgcaatga caatgaaaac cctgaaaaag ctacagggaa tttgctagtt aaagtcattt
      241 tatttttatg gaaccatttt cttaattata tcttactacc aaaggctccg aatccctttt
      301 aagtacaatg tgggcttccc aagacgggtc ttcattcggg aaatcctccc ttatatgcgc
      361 gcctcttgat tccgttctaa gaagggcaga tctagcaact aatgagcata catccaatat
      421 attttgtagc ttgagtttgt tatatccaac caattcatca cccgaactag ctaccttttc
      481 aaatacctca agcgcccttt taagtccata agcacttcga acaattccca catgttcaga
      541 cattatttga gaaataatac ttttgagctt aataaaacta tccctctgta agtcgggatt
      601 taaaataaag gataaatctt ttactagatt atccgattta tccgtcaatg tatcactttc
      661 tcgagctaaa tctaccgctc ttctcgcaaa cacaatacac tcaagaagag agttacttgc
      721 aagcctgtta gcaccgtgga ccccagtaca tgcgacttct ccacatgcaa aaagcccttt
      781 gatatttgtc tctgcccaaa gtccagtttt aacaccacct ataaggtaat gtgcagccgg
      841 agccacaggg attagatcac tggttatatc aagtccatac tgtaaggatg cattataaat
      901 atttgaaaat cgtttacgaa taaaatctgg gtttaagtgc tttaggctta aatagacata
      961 attcttgcca gatactttca tctcattaaa aatcgcgcgt gatacaacgt ctctgggtgc
     1021 tagctcagca agctcatgat attcgggcat aaacctatgt aatgaattat ttaataaata
     1081 tgccccttca ccccttacag cctcacttat gagaaaggtt tcaccgtttt ccgtgtaaaa
     1141 agcagtagga tgaaactgaa taaactccat atcagtaatt tctgctccgg ccctataacc
     1201 aagagcgatt ccatcaccag ttgcagtttg agggtttgtg gtccttagat agattcctga
     1261 ggcaccacca gttgcaagta ttgtagattc tgctttgaga actaagtgct tctgaatatt
     1321 aagatcgaac gctaaagccc caaaacacct ttctccatta gaaataagct cagctacttc
     1381 tgtgttttca aatataaata tatttggatt tgcttgaact gaagaaatta gaaactgcac
     1441 catttcttta cccgtagagt tccctcctgc gtgaagcacc cgtctcctac tgtgtccacc
     1501 ctcaagaccg agctctaacc ctttttcacc caaatcaaac ttcatcccta aagcaattaa
     1561 gtccgtgacc ctctctacac cttcacgcac gaggatttct acagcttctc tattacaaag
     1621 accccttcca gcctttattg tgtcatctag atgaaaaaga ggcgagtctt ccggatccat
     1681 agcagcggca ataccgccct gtgcccagta agaattgctt tcttccagcg tagatttagt
     1741 taggatagct actttgccaa atctagaggc gtaaagggca gcataaagtc ctgcaagccc
     1801 actacctatt actaagaaat cggaatgtag ataattaatg tttttggaat gtatgtcatc
     1861 cataaaattg agattaagac attctcccag atctattcaa gcaacaatat caattgcttc
     1921 aatcaatttg ttttcaatat tttctaaatt ctttttatca aaaaccttgc ctccctctat
     1981 ttcacgaacg taaaatacgt ctgccacctg gtcaacctta gtagaaatct ttgcataatc
     2041 tattgaaagc ccaagctttg taagtgtctt agtaatatca tacagaagtc ccaccctatc
     2101 atttgtgtaa atatccataa cagtagcagt atcagaggat tcgttgtcaa tatctatacg
     2161 tgtagggtgc tgaggaatgg gttttatata aataggcttc tgcctttttc tccttgacac
     2221 aagttgttca acatcaattt caccctttaa gactccatgc agattactct ttaccttgtt
     2281 ccatatttcc ttatcttcat aagtagattt gccaagcctg tttacataaa aaacatctac
     2341 aattcttcca tcgtcacggg taataatcct tgcccctaaa atatttatag agctcccagc
     2401 aagtactccg cagagttgtg aaaaaacccc aggtttatcg tatccccaga aggtgaactc
     2461 atcgtaacct tcgttctcat tataaattac atcacagccc gtgtcgtctc caaacccctc
     2521 gaataattta acgtgataaa caatcttctc tggagaaaat cccaagaaat aagtataggg
     2581 cataacatcc agatgtcgtc taatattaga ctcaggaatt tcattcttga gcagttgaat
     2641 aacctcacgt ataaatctcc tagatttcct cttgggtgtt tctctcttaa actctccacg
     2701 tgctagaaca tttgcggttc ctttataaag ttgttcaaga agcataccct tccagtttgt
     2761 ccaaacatcg ggtcccaccg atcttatatc agcgaaggag agaagataaa gcattgacaa
     2821 ggtttccaag gtctttacag accttgcaaa ccttaatatt aaaccgtcgt cgtgtatatc
     2881 acgtctttga gaaaagtgtg gcattacaag atgatgcttt actatgaatt cgagctgatc
     2941 gctttcttcc tctgtgagac ccattctttc agaaatccta ggaatcattc cagccccgcg
     3001 ctgggagtga tctcttcctt cccccttacc catatcatga aagaggcagg caagatatag
     3061 aatatggcgt ttagaaacct cttttgcaat ctgtgtaaca agtgggaatt gcttttcata
     3121 ttcacccttt ttaagattct cgatctcatt taccataaag attgaatgta tgtccaccgt
     3181 ataaacatga tatgcatcat gctgaaccat acaaactatt ttcccaaatt caggaataag
     3241 gtgtccaaga agacgaagcc tattcatgtc gaaaagggca ttggctacct ctttgccttc
     3301 tttaagaagc caaatgaacg aggcattaag cccaggattt cttctcacgt tatcgtctat
     3361 tatctttaca ttatcccgaa tcaagtcgtg gaggtacttg ctcatcttta ttccatacct
     3421 atctgctacc tcaaaagccc tgattagatt ttcaggatgc tcctgaaaaa tagtccgttt
     3481 cgaaactgaa agcattccac cttgaataat aaagctgtta tcgagaaaaa cagttttcgg
     3541 agtttgaaaa ctgacttttg gcttagaaac agatctttca acaagtttct ttgaatattc
     3601 ccttgtaaga tttgcgcgaa gataataaat cctcataaac ctctctacag cagcaagttc
     3661 acctacgtct cgaaaaccta gaaatctagc aaccttctcc tgccactcaa agctcaaacg
     3721 gtcttccctt ctccctgcga ggtagtgtag ctctgtccta atcggaagga ggaaatctaa
     3781 gcccttttca aaaaccctta gctccctttc aagcaaaaca ccctttttga ggagctccct
     3841 aaaacttcta actttgaact tagccttagc tacccatagg gcagaatgaa tatcccttag
     3901 tcctccctca ccttctttaa catggggctc tatgagataa acagacctac caaatctctc
     3961 tatccttttt tcattttctg cgatctttcc ttctataaac tttgatgata tttctggaag
     4021 gagctcttga tacattattc tgtcaagctg atcgaagagt ttcttgtctc caaagataaa
     4081 tctcccatca agcagagaag taaaaatcgt tgcatcatca ccacgagaga tttccatgca
     4141 gtcatcaata gttctaacag agtgcccgat ttctaggttt aaatcccaaa ggagataaag
     4201 aagagcttgg acagcttctt ttgctaaaga ctcgttccta ggcgcaaaaa ggaataatag
     4261 gtctacatca gaataggggc aaagctcatc ccttccatac ccaccaaggg caactattga
     4321 aacgccggat aatttagaaa acccaagccc tgttatggct ttactaatta gttcatctat
     4381 aacccgggat cttctctcag ctagttggcg accgcttaag gcttctttat cgtgcagctc
     4441 tcttaacttt tttcgatttt cttcaagata taatcgtatt ctgtccttga gtccatctct
     4501 tgctaataac tttcccgccc tttgcatctc ttttctttcc tttatccaca tagcgcacag
     4561 acttcgtcgt gagctcagtc gaatgaggtc acagaagatt ttaatattaa aacaaaattt
     4621 tttctccgtg ctctctgtgg ctaataaatc tcaaccctct ataacttcac acttaatgat
     4681 ataccatccc taatcggaat tatagtcgta agaaacccct tcttctcaaa aagaagacga
     4741 gtaaattctt taaccccttt tgtgctagga gataaatcat cagttaaaac cctaccaaac
     4801 cagagaacat tatccgttat aaaaaggcca ccgcgcctta attttttgta tgcttcatcc
     4861 acaactaagg ggtaatattc cttatcaatg tcgttaaaga tgatatcaaa ctgaccatca
     4921 gtttcttcca agatctgaag tgcatctcca acatgtattt ctactctatt gtcaagacca
     4981 ccacgtctaa aaaactcttt tgcaagactg gcattctttt gagaaagctc tgcaaacaca
     5041 acaagtcctt cgtc
//
LOCUS       DATDRL010000152         6791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1091158, whole genome shotgun sequence.
ACCESSION   DATDRL010000152 DATDRL010000000
VERSION     DATDRL010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091158"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..314)
                     /locus_tag="VI935_05570"
     CDS             complement(18..314)
                     /locus_tag="VI935_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25102.1"
                     /translation="MSMKIVTVEVLQCDHVDEKGVQCTSDGSRQAIKECSVCRKDLCS
                     RHYETFSLSRLSSLNSAGTSLTYFFCWEHAEEFINTLLKTLGDTRPVPYGGMAK"
     gene            complement(551..2257)
                     /gene="ilvD"
                     /locus_tag="VI935_05575"
     CDS             complement(551..2257)
                     /gene="ilvD"
                     /locus_tag="VI935_05575"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HLE25103.1"
                     /translation="MNSNQVKKKNGYRKYSSIYVDGAERAPSRSMLRAVGFKDEDFQK
                     PVIGIASTWSMVTPCNMHIDKLALEAGHGVDSSGGKSIIFNTITVSDGISMGTPGMRY
                     SLVSREVIADSIETVAGAEGFDGLVAIGGCDKNMPGCLIAMSRLNRPAVFVYGGTIMP
                     GAYCGKLVDIVSVFEAVGSHANMTINDNELKDIESCAIPGPGSCGGMYTANTMASAIE
                     ALGMSLPNSSAQAAISLEKNRDCFNAGDAVLSMIKNNIRPRDIMTKKAFENAITVVIA
                     LGGSTNAVLHLIAMAHAAGVKLSLDDFTRIGRKVPVLADLKPSGKYFMSEFCKIGGLT
                     PLMKMLLDEGLLHGDCLTVTGKTVVENLKDVKHYPNGQEIVASFNNPIKKDSHIVILY
                     GNLAKGGAVAKITGKEGLSFTGKAVVFDSEEACLKAILDGRVKKGHVIVIRYEGPKGG
                     PGMREMLSPTSAVMGKGLGQDVALITDGRFSGGSHGFVVGHITPEAYEGGVIAVVKNG
                     DIISIDAKKREINLLVPEKEIKRRLNALKRPKPKETRGALAKYARLVTSASEGAVTDK
                     NF"
     gene            2976..5147
                     /locus_tag="VI935_05580"
     CDS             2976..5147
                     /locus_tag="VI935_05580"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HLE25104.1"
                     /translation="MKESEIVKRVSITLLIILFTFLLFTFVGSMAKAENFCVGCPAPT
                     AANCDVAVSCPAGVNGGGVCPTIAAAVAAAELDPTVTAETIKIAQGIYTGPGSSDSIS
                     PPFGGMLTSKTINIRGGFNSTCSSRTVNASNTIINAPGGDRVFDIDSVTSTLNVTIDG
                     VTIQNGAPAAGNAGDGGGIRARATGVGGSLIFISTDNTYDTNATPGDGGGLSVEAGLG
                     SATALIDNNTFENNSAVNGGGASIEAVGGSIVATVTNNMIGFMGSPNTALVGGGIIFS
                     ADGGTLTVTNQNNIITSNTATAGPGGGIQAFADMSNLNVTISEDIISGNGADTTGGGI
                     SIENNTGSTTTAILEKNIINNNIADLSGGGINFFGSGTINATQVSNNMIFKNQAQGAS
                     PNGGGGVAASGSGNKTLKFVNNTIADNQATGSMATGGGILANASSGTINWTLPNDIIY
                     FNASPAAGEQIDISGAGTTTVTVRASVVQQGVGDLDPADVGMGADKINLIGTIPDDPD
                     FVDPPSNDYHLDSSSPAIDAGANAADPGEATIPTDDFENDPRPNNAGDPNDIGADEFT
                     PTPTPTPTPTPGPTPTPTPTPTPTPTPTPTPTPTPTPIPEETSCIDGEDNNGNGDIDC
                     ADDDCQQAAGGGLASTRQIGAEICELIETRCEDLFDNDGDGDLDCKDQDCAPVGVCEN
                     RGGCALSGPVTDIGSRIASILVPMLPIVGIGIRMLRRRLKK"
     gene            complement(5532..6173)
                     /locus_tag="VI935_05585"
     CDS             complement(5532..6173)
                     /locus_tag="VI935_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLE25105.1"
                     /translation="MKISEIFFSIQGEGVAIGLPNVFVRLFACDLRCTWCDSMYAVEG
                     TDFKEMSIEEVTAEIERFNCKRVCFTGGEPLIQRNEIEPLARDLVNDGYEIVLETSGH
                     KEPPPVFWTDSCLISMDCKCPGSGMEKRMDFELMEKLRSKDQLKFVIQDEADYKYAKG
                     ILKKYKIKANIVFQPVYGSSLKWLTERVLEDGLERVRVLPQLHKIIWGEIRGV"
     gene            complement(6183..>6791)
                     /gene="queC"
                     /locus_tag="VI935_05590"
     CDS             complement(6183..>6791)
                     /gene="queC"
                     /locus_tag="VI935_05590"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HLE25106.1"
                     /translation="VKEDYETYVLTFIYGQKHTREIKSAKKICEGLNVHHKVIDLSVL
                     KDVLSGSALTDSGVGVPEVPETAEHYETLKTTIVPNRNSIFLSIAIGYAVSIKANYIF
                     FGAHHSDRGVYPDCRREFVEAFEHAERLANDILYLVVSAPFVGMNKSEIVKLGAEMGV
                     PYKETWSCYKGGKVHCGVCSSCRERKRAFQEAAVLDPTEYER"
BASE COUNT         1908 a         1600 c         1350 g         1933 t
ORIGIN      
        1 aattcaacct tccaggccta tttcgccatc cctccatagg gaacgggtct tgtatcacca
       61 agggttttta aaagcgtatt aatgaattcc tctgcatgct cccagcagaa aaaatatgtg
      121 agagaggtcc cagcactatt gagacttgag agtctagaga gagaaaacgt ttcatagtgc
      181 ctgctgcata aatctttcct acacactgaa cattccttta tcgcctgtct cgatccatca
      241 cttgtacact gaaccccttt ttcatccaca tggtcgcact gtaatacctc aacggtcaca
      301 attttcatag acatgtcttt agtacccccc ttaaaatctg tattaataag gtgcataaca
      361 cttacttaaa ccctgataga ttatgataga aaattatcgc aactttatta gcaattaaac
      421 tagattttat taagtaagtc aagataagat acttgaattt tttgaatttt tcatggggtt
      481 agatctgtaa gaaaaattaa attacgccta acagtgcaga tgtgcaattt gtaaatcaaa
      541 aaaatagtaa ttagaaattc ttgtcagtca ctgcgccttc ggatgccgat gtgacaagcc
      601 tggcatattt agcaagagcg cccctggttt cttttggttt tggcctcttc aaggcattta
      661 atctcctttt aatctctttt tctggaacga gcaggttaat ctcgcgcttt ttagcgtcaa
      721 tcgaaatgat gtctccattt ttcacaaccg caataacgcc accttcgtag gcttcagggg
      781 taatatgccc cacaacaaaa ccatggcttc cacctgagaa tcgcccatcc gtaataagtg
      841 ccacgtcttg acccaaacct tttcccatga ctgcagaagt aggactgagc atttctctca
      901 taccgggtcc ccctttaggg ccctcgtaac ggataacgat aacatgtccc ttctttaccc
      961 ttccatccaa tatggctttt aaacaagcct cttcggaatc aaacacaacc gctttacctg
     1021 tgaatgataa cccttctttg cctgtaattt ttgcaacagc gccccctttt gctaaatttc
     1081 cgtaaaggat tacaatatgg ctgtccttct taataggatt attaaaagaa gctacgattt
     1141 cttgtccatt cgggtaatgt tttacatcct ttaagttttc tactacagtc tttccggtaa
     1201 cagttaggca atctccgtgt agtaatccct cgtcgagaag catcttcatc agaggtgtta
     1261 agcctccgat cttgcaaaac tctgacataa aatacttacc gcttggtttc aggtcagcta
     1321 gaaccggtac ctttcttcca atccttgtga aatcatctaa ggataattta accccagcag
     1381 catgagccat tgcaattaga tgtagcacgg catttgttga accacctaag gctatgacca
     1441 ccgtaatagc attctcaaat gcctttttag tcatgatatc acgaggacga atattatttt
     1501 taatcatgct aagcacagca tccccagcat taaaacagtc tctatttttt tcgagtgata
     1561 ttgccgcttg agccgaacta ttgggaaggc tcataccaag cgcttcaata gctgatgcca
     1621 tagtgtttgc tgtatacatt cccccacagg aaccaggtcc gggaatggca caggactcaa
     1681 tatctttaag ctcgttgtcg tttattgtca tattagcatg actacccaca gcctcaaaaa
     1741 cagaaacaat atctactagc ttaccacagt aagcgcccgg cattatggtt cccccgtaaa
     1801 caaagactgc aggacggtta agtcgagaca tagcaattaa gcaacctggc atattcttgt
     1861 cacaaccccc tatcgcaacg agtccgtcaa aaccctcagc ccctgcaact gtctctatag
     1921 aatcggcaat tacctctcgg gatacaagcg agtatcgcat accaggagtt cccatcgaaa
     1981 tgccgtcaga cacagttatt gtattaaaaa taatggattt accgcccgaa ctatccacac
     2041 cgtgtcctgc ctccaaagcc agcttgtcaa tgtgcatatt acaaggcgta accatgctcc
     2101 aggtagaagc gataccgata acaggctttt gaaagtcctc atccttaaat ccaactgctc
     2161 gtaacataga acgactcggc gcccgttcag ccccatcaac atatatggaa gaatattttc
     2221 tataaccgtt tttctttttt acctgattgg aattcatttt tttcctatcc ctcccaacaa
     2281 aactagcatt taacttgata attcctttta gcttgcttca gtgtttctac tgtcatcgcg
     2341 aacacattcg cggagcctgc agcggagagg cgggacattc ttgtcccgca acaggatgcg
     2401 gggtttgaaa acccagccta tcgaaatatt ccgtgaagca atccaactct ctttttgtca
     2461 ttgcgagcgt tagcgaagca atctgacgcg tagcgtcatc ctgaaggagc aaagcgactg
     2521 aaggatctcg tgagttatca tgggatcctt tgcatacgct caggacgaaa ctaagtgtcg
     2581 gatcgcttcg tcactgcctt ttatttcaat ttctcgttct tttcatttca ctcttaacca
     2641 cgctgcggtt caaagaatac tgcgctcgaa ataattctcc tcgcaatgac attctagtgt
     2701 tgcaatgacc accaagactg tctgtttata tttcgacacc attgctgcag ggtttttcat
     2761 tttatattgc acaattatta actggctaag ttttaatgtc aattgtttcc atagatacat
     2821 atcctgatat tttatcccaa gcacccaaca aagaaacaag gcttttttac gtaatgtcct
     2881 tgacaagccc ttattgtttt tcgtattatt gagcttaggt tttatttaat tagtaatggg
     2941 atacgaaatt tttgatttta gaggagtgga ggaagatgaa agaatctgaa attgtaaaaa
     3001 gggtatctat aactttactt ataattttat ttacctttct tttatttacc ttcgtaggct
     3061 ctatggctaa ggcagagaat ttttgcgtgg gatgtcctgc accaacagcc gcaaactgtg
     3121 atgttgccgt aagttgtcca gccggtgtta atggtggagg tgtttgccct acaattgcag
     3181 ctgccgttgc cgctgctgaa ttggatccaa cggttacagc agaaacaata aaaatagccc
     3241 aagggatata caccggacct gggagctcag actctatatc tcctccgttt ggagggatgc
     3301 ttacctcaaa aactataaac attcgagggg gctttaatag cacgtgctca agtaggactg
     3361 ttaacgcatc aaacacgatt ataaatgctc ctgggggaga cagggtattt gatattgatt
     3421 ctgtaacttc cacactaaat gtaaccatag atggggttac catccaaaat ggtgcacctg
     3481 ctgctggaaa tgctggggat ggcggaggaa taagagcaag ggccaccgga gtcggaggaa
     3541 gccttatttt tatatccact gataacactt atgatactaa tgccacacct ggcgatggtg
     3601 gagggctttc agttgaagcc ggtctgggct ctgcaactgc attaattgac aataacacgt
     3661 ttgaaaataa ttccgctgta aatggagggg gagcgtctat tgaagcggtg ggtggttcta
     3721 tagttgcgac agtaacaaac aacatgatcg gtttcatggg ttctcccaat actgctttgg
     3781 ttggtggagg aatcattttc tcggccgacg gtggcacact gactgtaaca aatcaaaata
     3841 atattatcac ctcaaacact gcaactgctg gtcctggggg tgggattcaa gcctttgctg
     3901 atatgagtaa ccttaatgta acgatttcag aggatattat ttcaggtaat ggtgctgata
     3961 ccactggtgg ggggatttct atcgagaaca atactggctc aaccactact gcgatacttg
     4021 aaaagaacat aatcaacaac aatatagcag acctctcagg aggtggaatt aatttctttg
     4081 ggtctggcac aataaacgca actcaggtat caaacaacat gatttttaaa aaccaagctc
     4141 aaggtgctag tcctaacggc ggtggaggag ttgctgcaag tggctcggga aacaagacac
     4201 taaaatttgt aaataacact atagcagata accaggcaac gggatctatg gctacaggag
     4261 gtggtatact ggctaatgcc tccagcggaa cgattaactg gacccttcca aacgatataa
     4321 tatatttcaa tgctagccca gccgcaggag agcagattga catttctggt gctggtacaa
     4381 caactgtcac agttcgtgcc tcggtagtcc aacagggggt gggcgacctt gacccggcag
     4441 atgtaggtat gggggcagac aagattaatc taataggaac aataccagac gacccggatt
     4501 ttgtggatcc tccaagcaac gactatcacc ttgattcatc ttctcctgct atagatgctg
     4561 gggctaatgc tgcagaccca ggggaggcaa caatacctac agacgatttt gagaatgacc
     4621 ctaggccaaa taacgctggt gatcctaacg acataggcgc cgatgagttt accccaacac
     4681 caactcctac gcctacccca acacccggac caacacccac ccctacccct acaccaaccc
     4741 cgacaccaac accaactcct accccaacac ctacccctac ccctattcca gaggagacct
     4801 cttgtattga tggtgaggac aacaacggaa atggtgatat agattgtgct gacgatgatt
     4861 gtcaacaagc agcgggaggg gggcttgctt caacgcggca gataggagca gagatatgcg
     4921 agttaattga gacaaggtgt gaagatttat ttgataacga cggagacgga gatctcgact
     4981 gtaaagacca ggattgtgct ccggttggag tttgtgaaaa taggggagga tgtgctctta
     5041 gcggacctgt aaccgacata ggttctcgaa tagccagcat tcttgtgcct atgctcccta
     5101 ttgtagggat aggaattagg atgctaagga gaaggctaaa gaagtaaaac gatgaggcaa
     5161 tccgtttaag attcctcact gctccttcgt ctgtcccttc gctttgctca gggcttcggc
     5221 tcaggacagg gtccggtctg acaccttttg tcagattact ttacggagta tattgataag
     5281 cggggtttcc aaaccccgca tcctgttgcg ggacaagcct gtcctgagtt taacgaagga
     5341 aatgtcccgc ctatccgtgc aggctacgcg aatgtgttcg caatgacaat ggaaactata
     5401 gcagcaagtt acagggaatt tgttagttaa tcgggtatcc ccccaacctg tcatacacga
     5461 atgcctttag tgggtaacat ttgggattta acggttttat tgactcgata gttctatgaa
     5521 gaaaactcaa ttcagacccc tcttatctct ccccaaatga ttttatgaag ttgcggaaga
     5581 actcttaccc tttctaatcc gtcttctaaa accctttctg taagccactt aaggctggag
     5641 ccgtaaacgg gctgaaagac tatgtttgcc ttaattttat atttcttcaa gatacccttt
     5701 gcatacttgt agtccgcttc atcctgaatg acgaatttaa gctgatcctt agagcgaagt
     5761 ttctccatga gttcaaaatc cattctcttt tccatccctg aacccgggca tttacaatcc
     5821 atacttatga gacagctatc agtccaaaat acaggtggag gctctttatg tccgcttgtc
     5881 tcaagcacta tctcatatcc atcgtttact aaatcccttg cgaggggttc tatttcattt
     5941 ctttggatta gtggttcgcc accagtaaag caaacccttt tgcaattaaa cctttctatt
     6001 tccgccgtca cctcttctat actcatctcc ttaaaatccg tcccctcaac tgcgtacata
     6061 gaatcgcacc atgtacacct aaggtcacat gcaaataaac gcacaaacac attaggcaga
     6121 ccaatcgcta caccctcacc ctgtattgaa aagaaaatct cacttatctt catcccatct
     6181 ttctaccttt catattctgt tgggtccaaa acggctgctt cctgaaaggc tctttttctc
     6241 tccctgcaag aactgcaaac accgcaatgt actttccctc ctttataaca agaccaggtc
     6301 tccttgtatg gaacacccat ctctgcacca agtttaacga tttcagattt attcatcccg
     6361 acaaacggag ccgatactac aagatataaa atatcgtttg cgaggcgttc tgcgtgttca
     6421 aaggcttcaa caaactcacg tctacaatcg ggatagacac ctctatctga atgatgcgct
     6481 ccaaagaaaa tataatttgc ttttatactt accgcatatc ctatagctat tgaaagaaaa
     6541 atcgagttcc tgttaggaac tatagttgtc tttaatgttt cataatgctc ggcagtttct
     6601 ggaacctcag gaactcccac gcccgaatct gttagagcag aaccagaaag aacatctttt
     6661 agaactgaaa ggtctataac tttatgatga acatttaaac cttcacaaat cttctttgca
     6721 gacttgattt cccttgtatg tttctgccca tagatgaatg tgagtacata ggtttcgtaa
     6781 tcctctttaa c
//
LOCUS       DATDRL010000153         4357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2222087, whole genome shotgun sequence.
ACCESSION   DATDRL010000153 DATDRL010000000
VERSION     DATDRL010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222087"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VI935_05595"
     CDS             complement(<1..354)
                     /locus_tag="VI935_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HLE25107.1"
                     /translation="MKRDYRLYLDDILEAIGKIEKYTKGLNEGQFKKDDKTVDAVIRN
                     FLVLGEAVKHIPQSIRRKYPNIPWKEMAGMRDKLVHEYFGIRHDVVWETISKRLPKVR
                     RLVETLLKKMDEEIKE"
     gene            complement(351..638)
                     /locus_tag="VI935_05600"
     CDS             complement(351..638)
                     /locus_tag="VI935_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HLE25108.1"
                     /translation="MIEKIISKLRQNKVILKGKYNVKSLQVFGSYVRGRQKKGSDLDV
                     LVEFYETIDLFKFMELEAFLSEKLGMKVDLVMKDTLKPRIKERILKEAVTV"
     gene            complement(635..3406)
                     /locus_tag="VI935_05605"
     CDS             complement(635..3406)
                     /locus_tag="VI935_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015197079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HLE25109.1"
                     /translation="MTPEEKTRQKIDQLLEAAGWIVQNLKELNLGASLGVAVREFTLE
                     SGEADYLLFVGRKAVGAIEAKPEGTTLSGVAEQSGKYLEGIPKNLPQVQQPLPFAYES
                     TSIETLFRDLRDPDFRSRRVFAFHKPETLLEWSSQKDTLRSLLKTMPTLIIEGLRGCQ
                     IEAIQNLEESFEEARPRALIQMASGGGKTYTAVSVIYRLIKFVNVRRVLFLVDRSNLG
                     RQTSREFQQYVTPDDGRKFTELYNVQHLTSNALDPVSRVCITTIQRLYSMLKGESEFD
                     AELEEQSIFDIAPIDEKPREVSYNPKIPIETFDFIVTDECHRSIYHLWRQVLEYFDAF
                     IIGLTATPSKQTLGFFNQNLVTEYSHERAVADGVNVGFEVYRIQTEITERGSRVESGY
                     YIDKRDRLTRKTRWELLDEELEYSPNQLDRDVVAPDQIRTIIRAFKDKLLSEIFPGRR
                     EVPKTLIFAKDDSHAEDIVHIVREEFGKGNEFCKKITYKTTGEKPEDLIASFRNSYNP
                     RIAVTVDMISTGTDIRPLECLIFMRDVKSRVYFDQMKGRGTRVISPTDFNAVTPDATR
                     KTHFVIVDAVGVCKNDKTDSRPLERKRTVPFDKLIMSVVLGNRDEDTLTSLAGRLARL
                     DREIDEKDRKEIEDAFVGAGFKPALTLKQIVNNLLDAVDPDRQMEKAKEIFKTEKPTE
                     EQVKKAAEELVKEACAPFDNPKLRNTIIDIKKRSEQIIDTVSKDTVISTGFDEKAKEK
                     ARTLVDTFKRFIEDNKNELTALQIIYSKPYGKRHLTYEQIKQLAEAIEKPPYKLNQEQ
                     LWLAYEQLEKSKVRRAGPQKLLTDIISLIRFAIGESDVLESFTETVNDRFYKWLSEQE
                     KSGRKFTPEQMEWLGMIKDHIATSLTIQMNDFEYAPFHEKGGVVKVYQIFGKELNSIL
                     EELNERLAA"
     gene            3593..>4357
                     /gene="pgk"
                     /locus_tag="VI935_05610"
     CDS             3593..>4357
                     /gene="pgk"
                     /locus_tag="VI935_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HLE25110.1"
                     /translation="MKMSISDMPDDFFKGKRVLVRVDFNVPIEKKMVREDYRIRRSIS
                     TIEYLAKRGAAIILASHLGRPKGKVVPELSLKPVADRLSEVLRVRDVKFVGDCVGKNV
                     KNIANQLLGGEVLLLENLRFHKEEEANDTQFSKELASLADMYVNDAFSTTHRNHASTY
                     GIASFFKHRLAGFLVQKEIEVLSKIRDNPDRPFKVIVGGSKIKDKIGALKNLINKADR
                     VLIGGAVAYTFLASKGVSVGDSPIEEDFLGWAKKVMA"
BASE COUNT         1177 a          938 c          821 g         1421 t
ORIGIN      
        1 ttcttttatt tcctcgtcca tcttttttaa tagagtctca actaaccgtc taaccttagg
       61 tagccgcttg cttatagttt cccaaacaac atcatgtcga attccaaaat attcatgaac
      121 taatttatcg cgcatccctg ccatttcttt ccatggaata ttcggatatt tccgccttat
      181 gctttgaggt atatgcttca ccgcctcgcc tagaactaaa aaattcctta taaccgcatc
      241 aacagtttta tcatccttct taaattgccc ttcattcaga ccttttgtgt atttttctat
      301 tttgccaatt gcctcaagaa tatcgtcgag gtaaagtcgg taatctcttt tcatactgta
      361 acagcctcct tcaatatcct ctcctttata cgcggtttca gagtatcttt catcacgaga
      421 tccactttca tgccgagttt ctcactcaaa aatgcctcga gctccatgaa tttgaacaag
      481 tcaatagttt cgtaaaactc tacaaggaca tctaagtcac tccccttttt ctgtctgcct
      541 cgaacataag agccgaagac ctgtagactt ttaacgttgt attttccctt gagaataact
      601 ttgttttgtc tgagtttaga gatgattttt tctatcatgc tgctaacctc tcatttagtt
      661 cctctaaaat actgttcaac tcctttccaa agatttgata gacctttacg accccgccct
      721 tttcatggaa cggtgcatat tcaaaatcat tcatctgaat ggttaagctc gtcgcaatgt
      781 ggtctttaat cattcctagc cactccatct gctcaggtgt aaatttgcgg ccggatttct
      841 cttgctcaga tagccactta taaaatctat catttacagt ttcagtaaag gattcaagga
      901 catcgctttc accgattgca aatcggatta acgaaataat atcggttaaa agtttttgtg
      961 gtcctgctct tcttaccttt gatttttcca gttgctcata agctagccat aattgctctt
     1021 ggtttagttt gtagggtggc ttctctatcg cctcggcaag ctgtttgatt tgttcataag
     1081 tgagatgacg tttgccgtag ggtttactgt agataatctg taaggccgta agttcatttt
     1141 tgttatcttc tataaacctc ttgaatgtat caacaagcgt tctagccttc tcctttgcct
     1201 tctcatcaaa gcctgtagaa attacagtgt ctttacttac ggtgtctata atctgctcgc
     1261 ttcttttctt gatatctata atcgtgtttc ttaattttgg attgtcaaaa ggcgcacagg
     1321 cctcctttac caattcttcg gcagccttct taacctgctc ctctgttggc ttctcagtct
     1381 taaaaatctc cttcgccttc tccatttgtc tatctgggtc aacggcatcc agtagattat
     1441 tgacgatttg ttttaaggtc agggcaggtt tgaaacctgc ccctacaaag gcatcttcaa
     1501 tttcttttct atctttttca tctatctctc gatcaagcct tgcaagcctt cctgcaagag
     1561 aggtgagtgt atcttcatct ctattaccca aaacaactga cataattagt ttgtcaaaag
     1621 gtacagttct cttacgctca agtggtcttg agtcagtctt atcattctta caaacaccaa
     1681 cggcatccac tataacaaaa tgcgttttac gagtggcatc cggcgtaact gcattaaaat
     1741 ccgtcggaga aataaccctt gtaccacgcc ccttcatctg atcaaagtag acccttgatt
     1801 taacgtctct catgaatatc aagcactcta acggtcgtat atccgtacct gttgaaatca
     1861 tatccaccgt tactgctata cgaggattat acgaattacg aaagctggca atcaaatcct
     1921 ccggcttctc gccggttgtt ttataggtta tctttttgca gaactcgttt cccttgccaa
     1981 attcctctct tacaatatgc actatatcct ctgcatgaga atcatctttg gcaaagataa
     2041 gggttttggg aacctccctt ctgccgggaa atatctcgct aagtagctta tccttaaagg
     2101 cccttataat tgttcttatc tgatcaggag ctactacatc cctgtctaac tgattgggag
     2161 aatattcaag ttcttcatcc agtaactccc atcttgtttt tcttgtgagt ctatcacgtt
     2221 tatctatgta atagccggat tcaaccctgc tccccctttc agtgatttca gtttgtatcc
     2281 tgtagacttc aaagccaaca ttcacgccat ctgcaactgc tcgttcatga ctgtattcag
     2341 taacaagatt ctgattaaaa aacccaagag tttgtttaga aggggttgca gttagtccta
     2401 tgataaatgc atcaaagtat tcaagcacct gccgccagag gtgataaatg gaacggtgac
     2461 attcgtcagt tacaataaaa tcaaatgttt ctatcgggat cttagggttg taggaaacct
     2521 ctctgggctt ttcatctatc ggcgctatat caaaaatgga ttgctcttca agctcggcat
     2581 caaattcaga ttcacccttt agcatagaat aaagcctttg aatggtggta atacacacac
     2641 gactcacagg gtcgagtgca tttgaagtga gatgctggac attgtaaagc tctgtaaact
     2701 ttcttccatc atcaggcgtt acgtactgct gaaactccct tgacgtttga cgcccaagat
     2761 tacttctatc taccaggaaa agaacccttc ttacgttgac aaatttaatc agccgataaa
     2821 taacactcac agcggtgtag gtttttccac cgcctgatgc catctgaatg agtgcccttg
     2881 gccttgcctc ttcaaaggat tcctcaaggt tttgtatagc ttcaatctgg caacctctca
     2941 atccttcgat aattaaagtc ggcattgttt tgaggagact tctaagggta tctttttgag
     3001 aactccattc tagaagagtt tctggcttgt gaaatgcaaa tactctccga gaacgaaaat
     3061 ccgggtctct caaatctctg aacaaggtct ctatgcttgt gctttcatag gcaaaaggaa
     3121 gaggttgttg gacttgagga agatttttag gaatgccttc taaatattta cctgattgtt
     3181 cagctacacc gcttagtgtt gtaccttctg gctttgcttc aatagcaccc acagcttttc
     3241 tacccacaaa aagtagataa tccgcttcac cagattcaag agtgaattcg cggacagcca
     3301 cacccaacga agctccaagg tttaattctt tgaggttctg aacgatccaa ccagctgctt
     3361 caagcagttg gtcaatcttt tgccttgttt tctcctctgg tgtcataata actccctata
     3421 tttttacacg agtgttggca atttcatata gtgtacatct ataactctat gaagaggcat
     3481 tcaaagggag agtttttgtt gataagatgt cttcacttgt tatttataca taaaataggt
     3541 ttagtaataa agatgtaaat aagaaaaagc ctgaatcggg aggggtcttt gaatgaagat
     3601 gtcaatctct gatatgccag atgatttttt taaggggaaa agagtgcttg tgcgggttga
     3661 ttttaacgtt ccgatagaga agaaaatggt aagagaggac tatagaatca gaaggtctat
     3721 ttcaactatt gagtacctgg ctaagagggg ggcggcgatt atacttgcgt ctcatcttgg
     3781 aagacccaag ggaaaggtag taccagaatt atctttaaaa ccggttgctg acaggctttc
     3841 cgaggtgctt agggttagag atgtaaaatt tgttggcgat tgtgtaggga aaaatgtcaa
     3901 aaacattgcc aatcaacttc tgggaggcga ggttttactt cttgaaaatc ttagatttca
     3961 caaagaagag gaagcaaacg atactcagtt ttccaaggaa cttgcatcat tagcagatat
     4021 gtacgtgaat gatgctttta gtactacaca taggaatcat gcttcgactt atggcatagc
     4081 ttcctttttc aagcacagac tggctggatt tttagtccaa aaagaaattg aggtcttaag
     4141 taaaataaga gataacccgg atagaccttt taaggtaatt gtaggcggct caaagattaa
     4201 agacaagata ggtgctctta aaaatttaat aaataaagcc gacagggttc ttataggcgg
     4261 agcggttgca tatacgttcc ttgcttccaa aggggtttct gttggggatt caccgataga
     4321 ggaggatttc ttaggttggg caaagaaagt catggct
//
LOCUS       DATDRL010000154         2501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_604282, whole genome shotgun sequence.
ACCESSION   DATDRL010000154 DATDRL010000000
VERSION     DATDRL010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604282"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VI935_05615"
     CDS             <1..823
                     /locus_tag="VI935_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25111.1"
                     /translation="KFTDAVEIAKETPTPGWRYFGIFAMLGVYVGVIPVYLGFLWFPF
                     LRRVSKKWMNFFLSLTAGLLIFLGADALHEAFEVAERTPGVFQGVALITIGTLLSFLI
                     LVIVGKRTIGSPSTQKSDLYMRLALAYMIAAGIGLHNLGEGLAIGSAYVLGEVALGAF
                     LVIGFAIHNITEGFGIVAPVASGEKPRNIHFLFLGLLAGVPAIFGTWIGGFIYSDIWA
                     TLFFAIGAGAIFQVVYGIIKLMNRESEAGIGNLTNFTGLVVGLLIMYITGLFVVI"
     gene            952..1740
                     /locus_tag="VI935_05620"
     CDS             952..1740
                     /locus_tag="VI935_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HLE25112.1"
                     /translation="MIFRKTRNRTLIIKLGWFLSIWVYYELGNQAKGEEEEWLPDRPD
                     VAESAFVVPPGRFQVEMGINIGQARGENEIGIITPFLFRYGFYKDFELRVGGQGLIRD
                     EGETNFSDLDLGIKYHLLDESAWIPAIGFIPQITLPTASRNAGSDSVDPRFFLTFDKD
                     LPLDFAIEWNLGVGYIDNPEDSGRFFQLLYAVSLGLSLVKPLTVFAEFYGVSKVEPGK
                     GGSINTDFGLLFRLANNVVIDLGGDFGITNTKQQNVFIGLSLRL"
     gene            2253..>2501
                     /locus_tag="VI935_05625"
     CDS             2253..>2501
                     /locus_tag="VI935_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25113.1"
                     /translation="MRVKTITLFLCSILIGITLESLKVTAEENQAQRKYKIDQLIELA
                     LEKNPSIPLALSSIDIAKGELRAAKAYPNPDLEFFLARG"
BASE COUNT          741 a          463 c          499 g          798 t
ORIGIN      
        1 taagttcact gatgcagttg aaattgctaa agagacgccg acacccggtt ggcgttactt
       61 tggaatattc gctatgctag gcgtatacgt gggtgtaatc ccagtatatc ttgggttcct
      121 ctggtttcct tttctacgaa gggtaagcaa aaagtggatg aactttttcc taagccttac
      181 agcaggtctt ttaatatttc tcggagctga tgcgcttcat gaggcatttg aagttgcaga
      241 acgcactcca ggtgtattcc agggagtagc attaatcact attggaaccc ttttaagttt
      301 tcttattcta gttatagttg ggaaaagaac gatcggctct ccatctaccc aaaaaagcga
      361 cctttatatg cgccttgctt tggcatatat gattgccgca ggtattggcc ttcataactt
      421 gggagaaggt ctcgcaatag gttcagctta tgttcttggt gaggttgcac tcggagcctt
      481 ccttgtaatt ggttttgcga ttcataacat caccgaaggt tttggaattg ttgcccctgt
      541 agcaagcggt gaaaaaccaa gaaatattca tttcttgttt cttgggcttt tagcaggtgt
      601 tccagcaatc tttggaacgt ggataggtgg gtttatatat tccgatattt gggcaacatt
      661 attttttgcc ataggtgcag gtgcaatatt tcaggttgtt tatggaatca taaagctcat
      721 gaatagagaa tcagaagctg gaataggaaa tctcactaac ttcacaggtc tcgttgttgg
      781 gcttctaata atgtatatca cagggctttt tgtagttatc taaaggaaat aatggtgatt
      841 caagatcagg gaaatataat actatgtctt aatataatcg cttcataata tcttacattc
      901 actccaacaa taaaaaataa atttccgctc gtaaagcaga agactgataa aatgattttt
      961 cgtaaaacta ggaatcgtac tcttataatt aagttaggtt ggttcttatc catctgggtt
     1021 tattatgaat tgggaaacca ggccaaagga gaagaggaag aatggttgcc agaccgccca
     1081 gatgttgcag aaagtgcatt cgttgtacct ccaggtcgct ttcaagtaga gatgggaata
     1141 aacattgggc aagcaagagg agaaaacgaa atcggtatta tcacaccttt tctattccga
     1201 tatggctttt ataaggactt tgaacttaga gtaggtggac aagggttaat tcgagatgaa
     1261 ggagaaacga acttttcaga tttagattta ggtatcaaat accatctgct tgatgaaagt
     1321 gcctggattc ctgccattgg tttcataccg caaattactc tacccacagc ctctcgtaat
     1381 gcaggatcag atagtgttga ccctcgattc tttctaacct ttgataagga tttacctcta
     1441 gatttcgcaa tagaatggaa cctaggagtt ggctatattg ataatcctga agattcgggc
     1501 agatttttcc aattgcttta cgctgtatcg cttggtcttt cgttagtaaa gcctctaact
     1561 gtctttgccg agttctatgg ggtaagtaaa gtagagccag gtaaaggtgg atcaataaac
     1621 accgacttcg gcttactctt tcgacttgct aataatgttg tgatagactt aggcggggat
     1681 tttggaatta ctaatacaaa acagcaaaat gtatttatag gtttatccct taggctataa
     1741 ataaagttag atgctaagtg gaattgacgt tttgtggaac aacgtttatc ttatttccca
     1801 actaaagcat ccaaagttaa agtaaggatg ttgaaacagg taacgaaaaa taaaattcca
     1861 atgaaggctc tcctatcagt aacccttttt ttcttctttt tttcagctac gacaatttgt
     1921 gaagagccct tcgtgcccaa tgtttaccat aaagattgtc tctcctctga acattgcctt
     1981 tcttctcaaa ttgataactc taagcacaat acttctacaa aatccccgct tccaatcagt
     2041 cattgtcata taggcagctc ctgtgctcat aattctacca aatctgaaag tttaaagcct
     2101 ttaattcaaa cagatacccc aactttaata atatctttta attactctat actagagagt
     2161 aacctaataa aatccatctt taaccctcca agaaccctca tttaataagt ccgtttttta
     2221 tcctacataa aatcgtttta aggaggttaa gaatgcgcgt taaaactata acgctctttc
     2281 tttgttctat tctaattggt ataacgctgg agagtcttaa agttacggct gaggagaatc
     2341 aagctcagag gaaatataaa attgatcaat taatagaact tgctcttgag aaaaatccat
     2401 ctataccctt agctctttcc agtattgata ttgcaaaagg tgagctgaga gctgcaaagg
     2461 cttatccaaa cccagattta gaattctttt tggcgagagg c
//
LOCUS       DATDRL010000155         3811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2661406, whole genome shotgun sequence.
ACCESSION   DATDRL010000155 DATDRL010000000
VERSION     DATDRL010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2661406"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VI935_05630"
     CDS             <1..516
                     /locus_tag="VI935_05630"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HLE25114.1"
                     /translation="EIGDCTRCKLHQWRTNIVFGEANPKARLVFVGEGPGEEEDKQAR
                     PFVGRAGQLLTKIITAMGLRRSDVYICNVVKCRPPGNRSPELDEAGTCEQFLFKQLRA
                     INPDVIVCLGSVAAQSVLRTKEKVGNLRGKFHSYGRAKVRVTYHPAALLRNPGYKKPL
                     WDDIQVVMKEL"
     gene            528..603
                     /locus_tag="VI935_05635"
     tRNA            528..603
                     /locus_tag="VI935_05635"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:561..563,aa:Arg,seq:ccg)
     gene            complement(627..1406)
                     /locus_tag="VI935_05640"
     CDS             complement(627..1406)
                     /locus_tag="VI935_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HLE25115.1"
                     /translation="MQTNPTEVIKESKDPSSEYEKASMLEDRYFLEGPRSRIQEFFFT
                     IRVLLEFIKGFRVLHFVGPCVTVFGSARLKEDHPYYQLARNVGSRLVKLGFTVMTGGG
                     PGIMEAANRGAKEAGGISVGCNIALPVEQKPNPYLDKWVTFNHFFVRKVLLVKYSYAF
                     VVMPGGVGTLDELFEVITLIQTKKIMDFPVVLISKEYFQDAMDFFKRMVVEGTIAAPD
                     LDLLLLTDSLDEAISHIEKHAVEKFKLRRRKLPKPSRLLRE"
     gene            1637..2497
                     /locus_tag="VI935_05645"
     CDS             1637..2497
                     /locus_tag="VI935_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25116.1"
                     /translation="MPKHKMQTIVITISNPKELTQGTSDSFFNPLVRPLRRVLENGVI
                     GRITYVLYQELNKPLRTFGAFCYTPGERLLFFPGIVKRRLRWNSEGKQKIEKFNLPTD
                     TLIDHFSLEKELDKWHITLLDINGEKKYPLRKYKTRRYQDNSLFWFGLSVKSPYVLDP
                     TPRESQFIFPSPETDSERRIQDIIKARDGAIFSLISLPDGLTLNVDEYLHFEVLVTKD
                     KRGGIDLPQFINFPTTPPSLESSVGMETQLTVRTHPVRLQGFPGSVFIVVAKHKGILS
                     NEAVITSPIE"
     gene            2589..2792
                     /locus_tag="VI935_05650"
     CDS             2589..2792
                     /locus_tag="VI935_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLE25117.1"
                     /translation="MRQVIIYPGEDGYWVAECPSLPGCISQGNSKEEWITNIKEAIRG
                     YIAALEEDGLPVPEEHFLVFENL"
     gene            2966..>3811
                     /locus_tag="VI935_05655"
     CDS             2966..>3811
                     /locus_tag="VI935_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HLE25118.1"
                     /translation="MKLKRRRLLSAFTAGIILGSIVSFTLSKEAILKASESKNTEKPN
                     RLINEKSPYLLQHANNPVHWYPWGEEAFEKARKENKPIFLSIGYSTCHWCHVMERESF
                     EDPEVAKLMNDVFVSIKVDREERPDIDSIYMTVCQMMTGSGGWPLTIIMTPDKKPFFA
                     GTYFPKESRFGRIGMTQLVPRIKEIWTTKRDEIVNSADKITSALQQATKESHGDKLDE
                     STLRVAYNQLAERFDTVHGGFGRAPKFPTPHNLLFLLRYWKRAGNEEALEIVEKTLQA
                     MRLGGI"
BASE COUNT         1162 a          717 c          831 g         1101 t
ORIGIN      
        1 gagatcgggg attgtacaag gtgcaaatta catcaatgga gaacaaatat tgtctttggt
       61 gaagcgaatc caaaggcaag gcttgttttt gttggtgagg gccccggcga ggaagaagac
      121 aaacaagcta gaccatttgt aggaagagca gggcagttgc ttacaaagat aattaccgcg
      181 atgggtctta ggcggtctga tgtatatata tgtaatgttg taaaatgtcg tccacccggc
      241 aacaggtctc ctgaactaga tgaagccgga acctgcgagc aatttttatt taagcagctt
      301 agagctatta acccagacgt aattgtctgt ttgggaagcg ttgccgctca atctgtatta
      361 agaacaaagg aaaaagtggg aaatctccgg ggcaagtttc attcttatgg aagggcaaag
      421 gtcagggtca cgtatcatcc agcagccctt ttaagaaacc ctggatataa aaaacccctc
      481 tgggatgata tccaggttgt gatgaaagaa ttatgaatct ttaaatcggg cgattagctc
      541 aggggataga gtgctggcct ccgaagccag aggtcgcggg ttcgaatccc gcatcgcccg
      601 ccattataaa atcacataac ttaagtttat tctctcagta accttgatgg tttcggtagt
      661 tttcgccgac gtagcttgaa tttttcaacc gcgtgttttt caatatgcga aattgcttcg
      721 tccaaagaat cggtcaaaag caaaagatct agatcgggag cagcgatggt tccctcaacc
      781 accatgcgtt tgaaaaaatc cattgcatcc tgaaaatatt ctttacttat aagtactacg
      841 gggaaatcca ttatcttctt tgtttgtatg agtgtaataa cttcaaagag ctcgtccaat
      901 gttccgactc caccaggcat cactacgaat gcgtaggagt actttacgag aagcaccttc
      961 ctaacaaaga aatgattaaa ggttacccat ttatccaagt atggattagg cttttgttct
     1021 acaggcagcg ctatattaca ccctacggat ataccccctg cttctttagc gccacggttg
     1081 gctgcctcca tgataccagg gccgcctcca gtcataacag taaatcctaa tttaacaaga
     1141 cggcttccta cattacgtgc aagttgatag taaggatggt cttcctttag acgtgctgaa
     1201 ccgaatacag taacgcatgg tcctacgaag tggagcactc gaaaaccttt aatgaactca
     1261 aggagaacgc gaattgtaaa aaagaactcc tgtattctcg aacgcggacc ttcaagaaaa
     1321 taacgatcct cgagcataga ggctttttca tactctgaag aagggtcttt gctctctttt
     1381 atgacttcag tgggattagt ctgcaatttt attcctcgtt agggtttgaa tataactcct
     1441 ataaaaaact atagttgaaa tattacctaa tctaaacgtt tagaactaaa ttaggaggtc
     1501 atggagttca gaccctccat cgctcgcaat gtaatatctt ttaatcacta gaagtatttt
     1561 tgcttttgat acttgctttt tcttttaagt atgctattgc aataattagt aacaatttag
     1621 ttaaattatt attttcatgc ctaaacataa gatgcagact atagttatca caattagtaa
     1681 tcccaaagaa cttacacaag gaactagcga ttcttttttt aatccacttg taagaccttt
     1741 aagaagagtt cttgaaaatg gggttatagg tagaatcacc tatgtacttt atcaagagct
     1801 gaataagcct ttacgaacat ttggggcttt ttgctacact ccaggtgaga gacttctctt
     1861 tttccctggt attgtcaaaa gaaggctaag gtggaattct gaaggaaagc agaagatcga
     1921 gaaattcaat ttaccaaccg atacattaat agatcacttt tccttggaga aagagttaga
     1981 caaatggcat attacactct tagatattaa tggcgaaaag aaatatcctc ttcgtaagta
     2041 caaaacacgg aggtatcaag ataatagcct cttttggttt ggcttgagtg taaaaagtcc
     2101 ttatgttcta gatccaacgc ctcgagagtc gcagtttata tttccttctc ctgaaacaga
     2161 ttcagaaaga aggatacagg acataatcaa agcaagagat ggtgctattt tcagccttat
     2221 ctctcttcca gatgggttga cgcttaatgt ggatgaatat cttcattttg aggttttagt
     2281 aacaaaagat aaaaggggtg ggatcgattt gcctcaattt attaatttcc caactacacc
     2341 tcctagcctt gaatcgtctg tagggatgga gacgcagttg acagttcgta ctcatcctgt
     2401 tcgtcttcag ggatttccgg ggagcgtttt catcgttgtt gctaaacata aaggaatact
     2461 tagtaatgaa gccgttatta caagtccgat agagtaaaag ttgactgagt tatttataga
     2521 cttttcttca aattactttt tacacaatct gacgttatac ttaatagcag cttgatggag
     2581 atgaaacaat gaggcaagta attatttatc ctggtgaaga tgggtactgg gtagctgaat
     2641 gccctagcct tcctgggtgt atcagtcagg gaaacagtaa agaagagtgg attactaaca
     2701 ttaaggaagc catacgagga tacattgcgg cattagagga ggacggttta cctgttccag
     2761 aagaacactt tttggttttt gaaaatttat aggtaataaa cccaacgtag ggtagtgcac
     2821 cagtcttgct ggtgtactac gcggcaacga gcaagactcg ttgcctaacc atgaaggtta
     2881 ttaatactag gttaatattt gagaaatcga gaaacgctgc cattggctgt atgagcaagt
     2941 taccgaatta cacggagttg ttagcatgaa gctaaaaaga agaagattat tatcagcttt
     3001 tacggctgga ataattctgg gatcaattgt atccttcaca ttaagcaagg aggcgatttt
     3061 gaaagcatct gaatcaaaaa atacggagaa acctaatcgg ctaataaatg agaagagtcc
     3121 ttatttactg caacatgcaa ataaccctgt acattggtat ccatgggggg aggaggcatt
     3181 tgagaaggct agaaaagaaa ataaaccgat ttttctttca attggatatt caacctgtca
     3241 ctggtgtcat gtaatggagc gcgagtcgtt tgaggaccct gaggttgcaa agctaatgaa
     3301 cgatgtattt gtttccatca aggtagatcg ggaagagcgc cctgatattg atagtatata
     3361 tatgacagtc tgccaaatga tgactggtag cggtggatgg ccgcttacta ttattatgac
     3421 acccgataaa aaaccctttt ttgctgggac ctattttcct aaagagagcc gttttgggcg
     3481 aattggaatg acgcaattag tgcctaggat taaagagatt tggacgacaa aacgtgatga
     3541 aattgttaac tcggctgata aaatcacctc tgctcttcag caggctacaa aggaatccca
     3601 tggtgataaa ctagacgaat ctactcttag agttgcatat aaccaacttg ctgagcgctt
     3661 tgatacagtt catggcgggt ttggtagggc tcctaagttt ccaaccccac ataacctact
     3721 tttcttgctt aggtactgga agcgtgctgg taacgaagag gctttagaaa tagttgaaaa
     3781 aacactccaa gccatgcgcc taggcggtat t
//
LOCUS       DATDRL010000156         4211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2973141, whole genome shotgun sequence.
ACCESSION   DATDRL010000156 DATDRL010000000
VERSION     DATDRL010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2973141"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VI935_05660"
     CDS             <1..140
                     /locus_tag="VI935_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HLE25119.1"
                     /translation="SAPKDFHSKRGLLQMVGKRRKLLRYLKRLSPGRYQNLLGKSGLR
                     K"
     gene            199..2310
                     /gene="pnp"
                     /locus_tag="VI935_05665"
     CDS             199..2310
                     /gene="pnp"
                     /locus_tag="VI935_05665"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HLE25120.1"
                     /translation="MVNELRRIKAQIFRRELEIETGRLAKQSHGAVLARYEGTAVLAT
                     VVAANEKTEGEDFLPLTVNYQEKAYAAGRIPGGYFKREGRPSEKEVLTSRLIDRPIRP
                     LIPMDFTFATQILVTVLSADQDNDPDVLSVTAVSAALLVSDVPFDGPAAAVRVGRING
                     ELICNPTRGELEKSEMDIVVAGTKEAIVMVEGGANEVPEQDIVEAILFGHRGIQEFIK
                     PQELLVSGLSLEKREMPTIVENSALAAGVVSFAKGRLREAIRVASKIERREGIQETLR
                     ETVQKLSPDFPGAENQVPKLFDVLKRDLVRELVIQEKRRIDGRGYADIRPISGEVSFL
                     PRTHGSALFTRGETQAAVVSTLGTTYDEQRIDALEGELTKRFMLHYNFPPFSVGEIGN
                     RLGPGRREIGHGALAERALLSVLPPKEKFPYTIRVVSEVLESNGSSSMATVCGGSLSL
                     MDAGVPIKAHVGGIAMGLIKEEDQFVILSDILGDEDQLGDMDFKVAGTLSGVTALQMD
                     IKVKGITREILTKALQQAYEGRVFVIGKMNEIIREPRPDISSFAPRILTIQVKPEKIK
                     DVIGPGGRMIKKIIEATGVEIDIEESGKVNIASSSKDACDKAAEMVKEIVQEIEVGKV
                     YLGTVKRVLDFGAIVELFHNTDGLVHISELAPTRVRKVSDIVKEGDEILVKCIGVEND
                     GKIRLSRKEALEENIEDYRKT"
     gene            2473..3423
                     /gene="miaA"
                     /locus_tag="VI935_05670"
     CDS             2473..3423
                     /gene="miaA"
                     /locus_tag="VI935_05670"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HLE25121.1"
                     /translation="MEVLISKNSVKDKPKLFVILGPTAVGKTKLGIEIAKRIGGEIVN
                     ADSLQVYRYLDIGTAKPLKEEHKQVPHHLIDIIDPDKEFNAGLYRKTAVERIIELHKK
                     STKMILVGGTYLYVRVLLYGIIDRISADMGIRGRLRKLKSTFGISYIYEKLKSFDSES
                     ASRIHPNDYVRIERALEAYYLTGSRMSELQEKHGFRENEYEVLKIGLFEEREILRKRI
                     DERVDMMLEEGLVDEVKKLREMGFGKDLKPMQSIGYKQINQYLDGGITLDRAVELIKR
                     DTKRLAKRQMTWLRKDKDIHWYHLPEVLEKIINEAEIFLN"
     gene            complement(3547..3906)
                     /locus_tag="VI935_05675"
     CDS             complement(3547..3906)
                     /locus_tag="VI935_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLE25122.1"
                     /translation="MVTIGSILIGLVAGIASGFFGIGGGLVMVPAMVYFFHLTQHQAQ
                     GTSLAVLTTPVVILGAIKYFKEGNVNLYMAAFIAVSFIIGAYLGAVLVHHVSDPMLKR
                     IFGVLLLIVSIKMILGK"
BASE COUNT         1303 a          711 c         1048 g         1149 t
ORIGIN      
        1 agtcagctcc taaggacttt cattctaagc gtgggcttct tcagatggtt ggtaaacgaa
       61 ggaagcttct tcgttacctc aaaagattga gtcctgggag gtaccagaat ctattaggaa
      121 agtcaggtct tagaaagtaa agagcaagat gcaggataca caatacatgc atcattaatt
      181 tgaggaggta gtatcatatt ggttaatgaa ttaagacgta taaaggctca gatatttaga
      241 agggagctag agattgaaac gggtaggtta gcaaagcagt ctcatggtgc tgttctagca
      301 cgttacgaag ggactgctgt tctggctact gttgtagcag ctaatgaaaa gacagagggt
      361 gaagattttc ttcctttgac cgtcaactac caggaaaagg cctatgcggc aggaagaatt
      421 cctgggggat attttaaaag ggaagggcgt ccaagcgaaa aggaggtact gacttcaagg
      481 cttatagacc ggcctatcag acctctcatt ccaatggatt ttacttttgc cacccaaata
      541 ttagttacgg tgctttcagc ggatcaggat aacgacccgg atgttctttc tgttacggcg
      601 gtttcagcgg cccttcttgt gtcggatgtt ccttttgatg gcccagcggc agcagtgaga
      661 gtaggccgga taaatggaga gcttatctgt aatccgacta gaggtgaact tgaaaaaagt
      721 gagatggata tagttgtagc tgggacaaaa gaggcgatag tgatggttga agggggtgca
      781 aatgaggtac ccgagcaaga tatagttgag gctattttgt ttggacatag aggcattcag
      841 gagtttataa aacctcaaga acttctggta agtggactaa gcttagagaa aagagagatg
      901 ccaactatag tggagaattc cgcccttgct gcaggtgttg tctcattcgc aaaggggcga
      961 ctaagagagg caatacgggt agcatctaag atcgaaagga gagaagggat tcaagagaca
     1021 ttgagagaaa ccgttcagaa actgtcccca gattttccgg gagctgagaa tcaagtacct
     1081 aagttattcg acgtactaaa gagggaccta gttcgtgaac ttgtaattca agagaaaaga
     1141 agaatagatg gaaggggtta tgcagacata aggcctataa gtggggaggt aagctttctc
     1201 ccaaggactc atggatctgc cctttttaca aggggtgaga ctcaggcagc ggttgtatca
     1261 actttgggaa cgacttatga tgagcagaga attgatgctc ttgaagggga gctgacaaaa
     1321 agatttatgc ttcactataa cttcccccca tttagtgtag gcgagattgg aaaccgcctt
     1381 ggtccgggtc ggagagaaat tgggcatgga gccctggcag aaagggctct tctttctgtt
     1441 cttccaccta aagagaagtt cccgtatacg attcgggttg tgtctgaagt gcttgagtca
     1501 aacggttcat cttcaatggc gacggtttgt ggagggagtc tatcgcttat ggatgcggga
     1561 gttcctataa aagcgcatgt tggtgggatt gcaatgggac tcattaagga agaagatcaa
     1621 ttcgttattc tttcagatat tctcggggat gaagaccagt tgggtgatat ggattttaag
     1681 gttgcgggaa ctctttctgg agtcactgcg cttcagatgg atattaaggt taaagggatt
     1741 actagggaga tactcacaaa ggcgcttcag caggcatacg agggaagggt ttttgtgatt
     1801 ggaaaaatga acgaaataat tagggagcca aggcctgata tttcatcttt tgctccacga
     1861 atacttacaa ttcaagtaaa gccggagaag attaaggacg ttataggacc aggtggaagg
     1921 atgataaaga agataatcga ggcgaccggg gttgagatag acatcgagga aagcggcaag
     1981 gttaacattg cctcttctag taaagatgcc tgcgataaag ctgcagagat ggtcaaagag
     2041 attgtacagg aaatagaggt agggaaggta tatctaggaa cagttaaacg agttcttgat
     2101 ttcggagcga tagtagaact cttccacaat acagacgggc ttgttcacat ttcagaactt
     2161 gctccaacca gggtgaggaa ggtttcggat attgtaaaag aaggagacga gatacttgta
     2221 aaatgtatag gagtagaaaa tgatgggaaa attaggctta gcagaaaaga ggcattagag
     2281 gaaaatatag aggattatag aaaaacctaa gcgattagtc gttagtcgta agtcgtagtt
     2341 ctttagaaaa tcatcagagt aatgtagcgg tgaccttcag ggcgccatgt agagacgtgc
     2401 catagtgaag gataggcggg aatttccagt cccgcatttg cggggttttc caacaccgcc
     2461 tatccttaat tcatggaagt tctcatatcc aaaaattcag tgaaggataa acccaaacta
     2521 tttgtcatct taggtcccac agcagtagga aaaactaaat taggcattga gatcgcaaaa
     2581 agaattgggg gggaaatcgt caatgccgac tcccttcaag tctatagata tctggatata
     2641 ggtacagcaa agccgctgaa agaagagcat aagcaagttc ctcatcacct aattgatata
     2701 attgatcccg acaaggaatt taacgccggg ttgtatagaa aaaccgcggt ggagagaatt
     2761 atagagcttc ataaaaaatc tactaagatg attcttgttg gtgggactta tctttatgtt
     2821 cgcgttcttc tttatggaat tattgataga atttccgccg atatgggaat tagaggccga
     2881 cttaggaagc ttaaatctac ttttggcatc tcttacattt acgaaaaatt aaagtctttt
     2941 gattctgaat ctgctagtcg gattcacccc aatgactatg taagaattga acgggcgctc
     3001 gaagcttact atctcacagg atcaaggatg tcagagcttc aagagaaaca tggatttaga
     3061 gaaaatgagt acgaagttct aaagattgga ctttttgaag aaagggagat tcttagaaag
     3121 aggatagatg aaagagttga tatgatgtta gaggaaggac tcgtcgacga ggtgaagaaa
     3181 ctccgggaga tgggttttgg aaaggattta aaacctatgc agtcaatagg ttataagcaa
     3241 ataaaccagt atctcgatgg agggattaca cttgatagag ctgttgagtt aataaagcgt
     3301 gacacaaaac gccttgccaa gcgtcagatg acgtggctaa gaaaggacaa ggatatacac
     3361 tggtatcacc tccccgaggt tttggagaag attatcaatg aggccgaaat ttttctaaat
     3421 tgaatattta tttgaacgaa taatcataca ctgtgtcatc ggtggttcgg gtttcgtgat
     3481 tcgtgtatag tgaatcttga atcaaatcac aaaccacgat ctacgaacca cggttctcta
     3541 cttaaattat tttcccaata tcatctttat agaaacaata agtagcagta caccaaatat
     3601 cctctttagc attgggtcgg agacatggtg aacaaggact gcgcctagat aagcaccgat
     3661 tataaaactc actgcgataa atgctgccat gtatagatta acattacctt ctttgaagta
     3721 ttttattgct ccaaggatta cgaccggagt tgtaagtaca gctagagaag ttccctgtgc
     3781 ctggtgttgg gtgagatgaa aaaagtagac catggctgga accattacaa gcccgccgcc
     3841 tattccaaaa aagccacttg ctataccggc tactaagcca atgagaatag atccgattgt
     3901 taccatttct taaacctcca acttgtattt atacgatgtg ataatggata aactttaaga
     3961 atttaaagag tcaaaattag ttaaaggatg ttatctaatt tatcgctatt tctacttggt
     4021 ttgtcgtagt tatataagta ttattgaaaa tcgactttca agtcgttact tgactctcta
     4081 ttttgtagtt gtttgtaaat tgcttccgcc tcactagact tacccaattt ttctagtgag
     4141 aatgcaatat ggagtttggc ttgattccta tatggatttt tctctttatc aagttttgat
     4201 agataatcta t
//
LOCUS       DATDRL010000157         4380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2727942, whole genome shotgun sequence.
ACCESSION   DATDRL010000157 DATDRL010000000
VERSION     DATDRL010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2727942"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2183)
                     /gene="glnE"
                     /locus_tag="VI935_05680"
     CDS             complement(<1..2183)
                     /gene="glnE"
                     /locus_tag="VI935_05680"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HLE25123.1"
                     /translation="MNKVLPDPENAENVIKRFIEVHKSISPHRKDLLYTLSSYSRFLG
                     RVIIKDPEILDILVKTEFVQRKKTLEQALQDSTRIRESSNSPEEFMAQLRRYKYREFS
                     RIVYRDIMNMGSFEETMEELSDLASSVLEAAFRFFEKELKATSHGKFVIMGMGKLGGM
                     ELNLSSDIDLIYIYQTTRKQDPSPELRTGPFFKIAERITRALSSVTEDGFLYRVDLGL
                     RPGGGKSAIALSFDGALEHYFYWGDTWERAALIKARPVAGDISLGDDFNKEIEPFVFR
                     KHLDYTSIEDLKDMKAKLDRLHKVRDVKLGKGGIREIEFFVQALQLVNGGAIKELREK
                     NTLMALERLSERKIIEDEVFQSLSSCYLFLRKVEHAIQLADELQTHKVPEDSHGLDKL
                     AKRVGFQNKEEFEKEYNERTSEVSKIYGRLFHEPSKRIEEGGKAFWELADFLTEGNIA
                     QSEAIEDLRRLGFKNPESALELIAALLDPKKGGLTHRGRILTRRVVPAFLGRVIASPD
                     PDAALVNLERFISGIGWRTSIFAVLAENPELLELLSRLFSTSGYLSNFLIRHPEYLDV
                     LTLRDVIKEFGSKEEMVLELKKILSEEKEYEGKLDALRRFKHVETLKLCLRDLNREVD
                     PIYVGNFLSMLAESVLEAGLLLAGEVLGSTLGRRGTPGDIIILGMGKLGGMEMSYNSD
                     LDIIFIYKGDEHEFFSKLGQKVISILSIPTNEGFAYKIDTDLRPSG"
     gene            complement(2176..2460)
                     /locus_tag="VI935_05685"
     CDS             complement(2176..2460)
                     /locus_tag="VI935_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25124.1"
                     /translation="MKKKNLSDLEKAREHFLKAFTETIIGADFAIKGARNLLRQRKGR
                     RRRMVGLTESLIRKGFNLATKLSDVLTQVRSEKPAKQRARRKKMRKVEIE"
     gene            complement(3085..3384)
                     /locus_tag="VI935_05690"
     CDS             complement(3085..3384)
                     /locus_tag="VI935_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HLE25125.1"
                     /translation="MSKRELGPIHPGEVLLEEYLKPMGISQYRLAKEINVDPRRINAI
                     VNGERAITADTALRLSRYFGTSERFWLNLQAHYELEVQKDELGDRLKREVKALMA"
     gene            complement(3395..3679)
                     /locus_tag="VI935_05695"
     CDS             complement(3395..3679)
                     /locus_tag="VI935_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLE25126.1"
                     /translation="MIREFADSDTEKLYRGQRPRKLPTQMLRPALRKLLILDAAESLV
                     DLRIPPGNRLEKLKGDREGQYSIRINDRWRICFRWRGGDAYAVEITDYHS"
BASE COUNT         1210 a         1085 c          790 g         1295 t
ORIGIN      
        1 cccgatggtc ttagatctgt atcaatctta taggcaaagc cctcgtttgt tggaattgaa
       61 agaattgata taaccttctg cccaagtttt gagaagaatt cgtgttcatc tcccttgtag
      121 atgaaaataa tatcaagatc agaattgtag ctcatctcca taccacccag ttttcccatt
      181 ccaagtatta ttatatctcc aggtgtcccc cttcttccca atgtagaccc taaaacctct
      241 ccggcgagca aaagtcctgc ttcgagtacg gattcggcaa gcatagatag aaaatttcca
      301 acgtagattg gatctacctc gcgattcaaa tccctgagac agagcttaag tgtttctaca
      361 tgcttgaacc ttctaagcgc atcaagcttg ccttcgtatt ccttctcttc acttaagatt
      421 tttttaagct ctagaaccat ctcttcctta gagccgaatt cctttataac atccctaagt
      481 gtaagtacgt caagatattc aggatgcctt ataaggaaat ttgaaagata accactggtt
      541 gaaaaaagcc tggaaagaag ctcaagcaac tctgggtttt ccgcaagcac ggcaaaaatg
      601 gatgtacgcc acccgattcc agaaataaat ctctcaaggt ttacaagtgc agcatcaggg
      661 tcaggagaag caataaccct gcctaagaat gcgggtacta ccctccttgt aagtatcctt
      721 cccctatgtg taagcccgcc cttttttggg tcgagaagcg cagcgataag ctcaagtgcg
      781 ctttcaggat ttttaaatcc caaccttctt aaatcctcta ttgcctcact ctgagcgata
      841 tttccctccg ttaaaaaatc ggcaagctcc caaaaagcct ttcctccttc ctcgatcctt
      901 ttggagggtt cgtgaaaaag ccgtccgtaa atcttagaga cctcggatgt cctttcatta
      961 tattccttct caaattcttc tttattttga aaccctaccc tttttgctag tttatcaagt
     1021 ccatgagaat cttctggcac cttatgagtc tgaagctcat ctgctaactg aatcgcatgt
     1081 tcgacctttc ttaaaaaaag atagcaagaa ctgagtgatt gaaatacttc atcctcgata
     1141 atctttcttt ccgagagcct ttcaagagcc attagggtat tcttttctct tagttctttt
     1201 atcgccccac cgtttacaag ttgtagcgct tgcacaaaaa actctatttc ccgtatacct
     1261 cccttaccaa gctttacatc gcgtacctta tggagtctat ctagttttgc cttcatgtcc
     1321 tttaaatcct caatggatgt gtaatcaaga tgcttcctaa aaacaaaagg ctcgatctct
     1381 ttattgaagt catctccaag agagatgtcc cctgcaacag gtcttgcttt aataagcgcg
     1441 gccctctccc aggtatcgcc ccagtaaaag taatgttcaa gtgcgccatc aaatgaaaga
     1501 gcaattgcgc ttttcccgcc accggggcga agtcctaggt caaccctata aagaaaacca
     1561 tcttcggtaa ctgaactaag cgccctagta attctctccg caattttgaa aaatgggcct
     1621 gtcctgagtt ctggcgaagg atcttgtttt cttgtggtct gataaatata gattaaatct
     1681 atatccgagc tgaggttgag ttccattcct ccaagcttcc ccatacccat aattacaaat
     1741 tttccgtgac ttgttgcttt aagttctttc tcaaaaaatc taaacgccgc ttctaaaaca
     1801 ctactagcta aatctgagag ctcttccatt gtctcttcga aacttcccat attcatgatg
     1861 tctctataga cgattcttga aaactctcta tacttatacc gccttaattg agccataaat
     1921 tcttctggag aattagagga ctccctgata cgggtggagt cctggagtgc ttgctctagt
     1981 gtttttttcc tttgaacaaa ctccgtttta actaaaatat ccagaatctc agggtctttt
     2041 attattaccc ttccgagaaa tcggctgtag gatgagagag tataaaggag atctttcctg
     2101 tgaggagaaa tactcttatg aacctctata aaacgcttta taacattttc agcgttttca
     2161 gggtccggaa ggaccttatt caatttcaac cttcctcatc tttttcctac gtgctctttg
     2221 cttggctggt ttctctgatc gcacttgagt taaaacgtca gagagctttg ttgctagatt
     2281 gaagcccttt ctaatcaagc tctccgtaag accaaccatc ctcctcctcc tccccttcct
     2341 ttgccttaaa aggttccttg cacccttaat agcaaaatcg gctccaatta tggtttcagt
     2401 gaaagccttt agaaaatgct ccctagcctt ttctagatca cttaggtttt ttttcttcat
     2461 atctcaattt taacataaat ttgtttaatt ttaacccaac aaaaatgcag gcgaagggac
     2521 aggtagagga gcagtgagaa ataaggaatc tcacgagatc atttcgtttt cactcccgat
     2581 ggcatcccgt aggatcccca tcccttcccc gatctaatcg gagaccaagc tcaggacagg
     2641 caccctggcg caaccctatg tgtagagacg ccattaatgg cgtctctaca gcgctcctgt
     2701 cataccgaac acgttcgctg agcaagtctc cacactcgat aggtgggact ttccagtccc
     2761 acccttccac aagcaagtac gtgtatcaac gtaggcgggg ttagaaaacc acgcctatct
     2821 cgtcacaaca caaaaatggt tttcattgcg aacacttcga taaactcaat gtaaactccg
     2881 tgaagcaatc cctaacatca ccgggtaggt cacgagttcc ttcgacttcg ctcaggacag
     2941 gcttgctcgt gacccattgt ctacccattt attccaataa cgataggcgg gactttccag
     3001 tcccgctccc ttctgcggcc aaacaactat ttaaaatagt ctctgtccct ataaaagact
     3061 attcctataa aacactattg catgctaagc cataagggcc ttgacttccc tcttaagccg
     3121 gtcgcccagt tcgtccttct gcacctcaag ttcataatgc gcttgcagat tgagccaaaa
     3181 gcgctctgat gtcccgaagt aacgagacag ccgcagagcc gtgtcagccg tgatggcccg
     3241 ctcgccattc acaatcgcgt tgatccgcct cggatccaca ttgatctcct tagccaaacg
     3301 gtactggctg atacccatgg gcttcaggta ctcttctagc aggacctccc caggatggat
     3361 tggacccaat tcccttttac tcatatgtac ctccttagga gtgataatcc gttatctcca
     3421 ccgcgtacgc gtcgccaccc cgccatctaa aacagatccg ccaccgatcg ttgattctga
     3481 tactatattg accttctcga tctcctttaa gcttctccaa cctgtttcct ggcggaattc
     3541 gcagatccac cagcgactcg gccgcgtcca ggatcaggag cttgcgaagc gccggacgca
     3601 acatctgcgt tggcagcttc cgtggccttt gtccccgata cagtttctca gtatctgaat
     3661 cggcgaattc cctgatcact aaggtatata atagcgcaac acactattag cgtcaagcac
     3721 cgtgcaggaa caaaagaaac cacctaacca ttaatatact aaaaatgttt cgcaaaatta
     3781 cgaaatatta tgtgataatc caaacagcta aagtgggttt cttctcagcg ttagctagag
     3841 gaacaaagat gataaacaaa actaaactaa gtaagtaccc tctgttctcc aatttactca
     3901 cgcaagaagg gtatcaaaac tttctttata gcttatggct atatctgaaa taaaaatgga
     3961 gtctgttgtc cctatttttc gtatgacaaa aagttgttat ttcaatgaca gcacagacga
     4021 gccctgtagc aagtgagttc tagtgttaaa aatagtgtca accactattt tttgtaaatt
     4081 ttatataatt tatagattta tacacatcaa ataaataatt tgccctctaa ttgcagcata
     4141 tttaatcata ctgattattt tataaccagt tttagggtca gggttaggga ggggggtcct
     4201 aggactaaag ttgtagaacc aaaattatag aaaaatagac tgtcaataca aagtgaatat
     4261 gtcacccctg atgcaaagta aaaatgtcac ctgtaagtag attctcaagg ggtagagtat
     4321 gagttttaat ctttttagtt tcaccagaac taatctcctt aaaattcaga tattgatctt
//
LOCUS       DATDRL010000158         2714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_558915, whole genome shotgun sequence.
ACCESSION   DATDRL010000158 DATDRL010000000
VERSION     DATDRL010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558915"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VI935_05700"
     CDS             complement(<1..366)
                     /locus_tag="VI935_05700"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HLE25127.1"
                     /translation="MKVIFDPEVPLKFSSSATIGIFDGVHIGHRRIIGLLRKDAREKG
                     ISSCVITFHPHPQKVLRGIDIPLIVPLKERFRLLEKEGVDVTVCYSFTKEFASVQAED
                     FVRDMLVGRLGIRTIFVGPD"
     gene            complement(363..1037)
                     /locus_tag="VI935_05705"
     CDS             complement(363..1037)
                     /locus_tag="VI935_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HLE25128.1"
                     /translation="MKKGKFLVIGLGNFGSNLARRLFERGQEVIAIDLNDKKIIEAKD
                     YVSEAVVADATQRETLEQLGLGTIDTAVISLGEDRIDASVLATLHLRDMGIKNIIVKA
                     ISPDHARIVETIGATEIISPEKEAALRLAERLSTPHVFEQINLHEGYTLVEMMCPKEL
                     WNKTLKDAGIRSKYGLTVVWIKRKKPDGKEISMLPRPDDIVIEGDVLFILGEEKAIEG
                     FKKLKT"
     gene            complement(1034..2374)
                     /locus_tag="VI935_05710"
     CDS             complement(1034..2374)
                     /locus_tag="VI935_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HLE25129.1"
                     /translation="MSRPQEQTKELNPTQLIILTFAAACIAGGLLLEIPIATHEDISF
                     IDAVFTATSAVCVTGLVVVDTGTAFTDFGKLVILLLIQVGGLGITTYGVVFTSVLTGR
                     ISLKDRAMIRDSFAHFGVVNFRHLLYEIVIFTLLTELLGAIFLFIFIPYDGFFTALFH
                     AVAAFCNAGFSFYRDSFTGYRGSVGVNLVLVWLIIMGGIGFLTVRDLRLYVLKKIPQI
                     SLHSRIVLLVSAFLLIAGTFAVLIFEWDNALVGMPIKEKVLTSLFQSASARTAGFNTV
                     DLTLLSSSTLLVLIILMFIGTSPGSCGGGVKTSTLAVMLALAKNRSLGRDEVFLFKRT
                     IPKDIVSKALTVVTASLLVIILMSLLLTASEGWGNPYTKMPGAFMDALFETVSAFGTV
                     GLSVGNTAELSTFGKIVVIFTMFIGRVGPLALALAVGGEKAIRYKYAEERVMVG"
     gene            complement(2495..>2714)
                     /locus_tag="VI935_05715"
     CDS             complement(2495..>2714)
                     /locus_tag="VI935_05715"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HLE25130.1"
                     /translation="WKIRKRDLPIGWLSGVTLILMGLERFLVEFVRNTTPSFIPGVSL
                     AQLMSLGLIVVGVLKLLQLRMGTKTLRV"
BASE COUNT          846 a          634 c          480 g          754 t
ORIGIN      
        1 atccggaccg acaaatatag ttctaatgcc aagtcttcca actagcatat ctctaacaaa
       61 atcctcggcc tgaacagatg caaattcttt tgtgaaactg tagcacacag taacatccac
      121 accctctttt tcaagaagcc tgaacctttc tttgagcgga actataagtg gaatatcaat
      181 cccgcgtagc accttttgcg gatgggggtg gaaggttatc acacaagaac taataccctt
      241 ttctctagca tcctttctca gaagaccaat aattcttctg tgcccgatgt gaactccatc
      301 aaaaattcca attgttgcag agctagaaaa tttaagaggt acttccgggt cgaagatcac
      361 tttcatgtct taagcttctt aaacccttca attgcctttt cctcaccaag aataaaaagg
      421 acatcaccct cgataacaat gtcatcaggg cgtgggagca tcgaaatctc ttttccgtct
      481 ggtttttttc tctttatcca gacaacagtc aatccgtatt ttgatcttat ccccgcatct
      541 ttaagtgttt tattccatag ctctttcggg cacatcattt caactaatgt gtatccctca
      601 tgaagattaa tttgctcaaa tacatgtgga gtgctcaatc tttctgcaag tcttagagcg
      661 gcctcttttt caggggatat aatttcagta gcaccaattg tttcgactat ccttgcatgg
      721 tcaggagata ttgccttcac gattatattt tttatcccca tgtcccttag atgaagggta
      781 gcaagcacac tggcgtcaat tctatcttct ccgaggctta tcactgctgt atctattgtt
      841 ccgagaccaa gttgctctag agtttctctc tgagtggcat cagctacaac ggcttcagat
      901 acataatctt tggcttctat gatcttctta tcgttaagat caatagctat tacttcctgt
      961 cccctttcaa aaagccttct agccaaattg gaaccaaagt ttccaagccc aataactaga
     1021 aacttaccct ttttcatcca accatcactc tctcttctgc atatttatac cgaatggctt
     1081 tttctccacc tacagccaaa gcaagagcaa gggggccaac tctcccaata aacatagtga
     1141 atattaccac aatcttgccg aaagttgaaa gctccgcagt atttccgacc gatagaccta
     1201 ctgtcccaaa agcagagaca gtttcaaaaa gcgcatccat aaaagcgcca ggcatcttag
     1261 tgtaagggtt tccccaaccc tctgaagctg ttagtaatag agacattaaa attattacca
     1321 ataaacttgc cgttaccaca gtaagagctt tcgacacaat atctttggga atggttctct
     1381 taaaaagaaa aacctcgtct cttcccaacg aacgattctt tgccaaagct agcattactg
     1441 ctaatgttga ggttttcact ccgccgccac atgaaccggg ggatgttccg ataaacatta
     1501 aaattatcag gactaaaagt gtggaacttg atagcaacgt aaggtcaaca gtgttaaacc
     1561 ccgctgttct cgcactagca gactgaaaga gcgaagtaag caccttctct tttatcggca
     1621 tacctactag agcgttatcc cattcaaata tgagtaccgc aaacgttcca gctataagca
     1681 gaaaagcaga gaccagaagc acaatcctcg agtgaagtga gatttgtgga atttttttaa
     1741 gaacgtatag cctgagatcg cgaactgtga gaaacccaat tccacccatt atgataagcc
     1801 agacaagaac cagattaacc ccgacgcttc ctctataccc tgtaaagcta tcccgataaa
     1861 acgaaaatcc ggcattgcag aaggcagcta cagcatgaaa gagtgcagta aaaaaaccat
     1921 catacggaat aaatataaaa agaaatatcg caccaagtaa ttcggtcaat aatgtaaata
     1981 taacgatctc atacaataga tgcctaaagt ttaccactcc aaagtgagca aaagagtcac
     2041 gtatcattgc tctatcctta aggctaattc gtccagtaag tacagaagta aatacgactc
     2101 cataggtagt tattcctagg ccacccactt gtattaaaag aagaatcaca agttttccaa
     2161 aatctgtgaa tgcagtccca gtatcaacaa caacaagccc tgtgacgcac actgcggagg
     2221 tagcggtaaa aaccgcgtcg atgaacgata tatcttcatg agtagcgatt ggaatctcta
     2281 ggagaagtcc tcctgcaata cacgcagccg caaatgtaag aataataagc tgcgtagggt
     2341 ttagttcctt ggtttgctct tgtggtcttg acatctttta ggttggagaa atattatcca
     2401 ctcaaaaata aaaagttcaa aacctaattt caatctagct gttggagcaa aatgaagaat
     2461 aaataataag cggatcagct aaactcgttt ccttttaaac tctaagggtt tttgttccca
     2521 ttctaagctg tagaagtttt aagaccccca caacaatcaa cccaagggac ataagctgag
     2581 ctaaagaaac ccccgggata aagcttggtg tggtatttct tacaaactct acaagaaatc
     2641 tttctagtcc cattagtatt agcgtgactc cagaaagcca gccaataggt aaatctcttt
     2701 tgcgaatctt ccat
//
LOCUS       DATDRL010000159         4287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1854736, whole genome shotgun sequence.
ACCESSION   DATDRL010000159 DATDRL010000000
VERSION     DATDRL010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854736"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VI935_05720"
     CDS             complement(<1..790)
                     /locus_tag="VI935_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HLE25131.1"
                     /translation="MKKKREHYLHSPVTQPSVRKLKCIGDYVNAFSNMSFQARALGEG
                     AKTLLEMVKTPNCLKTLTIAGAPVPGGLQGVICDLIELKFIDVIITTGSQIAHDMVEA
                     LGFSHHKGHHMMNDLELRDLRIDRMYDSLLDETKLNEVDLLLNELWGQEGSRIISTPE
                     LCKRIATYILGKGLYKGQSFVVSALKSGIPIFSPTIHDSEIGISLTTFNKKHPKKRVV
                     VDLLLDNEKYIEIFLDRKYKKRGILILGGGVPRNWAQQVTPMIEF"
     gene            1595..2566
                     /locus_tag="VI935_05725"
     CDS             1595..2566
                     /locus_tag="VI935_05725"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HLE25132.1"
                     /translation="MKCPKCGFNSFEYLDSCKKCGSALGVNPRYKVIYGSFEKDKGQE
                     DVNLAESKISKEEIGKPIISTPSVLISSPQESLESKKREEVIKSTFPPSGYYSLPQET
                     PTFIEEAKRGEEIAVSERKVSKEEIEKSIVSTSSVLVPPSEESLKSKKSDEELVKSPL
                     FTSSYYSSPQESPAFAESEELLPFNLAGMGLRIGAFLLDIIILFMVTTLTLWIGFFLG
                     SIVFNIREEKFINVIVPLYLILFFLSTTYFVFLQGFSGKTIGKMLFGIRVIRTDGESI
                     GFWDALIRWVGYFISLIFIFIGFIWAIFDPKRQAWHDKFAETYVVKE"
     gene            2573..3835
                     /gene="gshA"
                     /locus_tag="VI935_05730"
     CDS             2573..3835
                     /gene="gshA"
                     /locus_tag="VI935_05730"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HLE25133.1"
                     /translation="MRFLDEINYRLNEKAKEIEDWIDEQSRKARVPLYTSVDLRVSHH
                     KITPVDTNIFPAGFNNLCRVFRERVGRLFREEFIRSHPETRKIVVVPELHTRNPYYWE
                     NIQVIKTILESEGFLVEIGLVSDELYQDGIGFKAISGKEVKAYKLKRKDGIVYISEFI
                     PDLILINNDFSSECPEILRDISQPVEPPVEMGWHTRRKNVHFEFYNKLAGELADIIEI
                     DPWMISIETFSQKGVDLDKPEDREIVATHVDLLLNRLRNEYEKRGVNEKPFVFVKSNS
                     GTYGMSVLSVLAGDDIRTLNAEGRKQMKVTKGGKPVRDVVIQEGIPTSLKFEPQVVGE
                     PVIYLIKSNVAGGFLRQNKGKSKFDNLNTKGMEFSCLSFENENSCENKECEKFLPPAY
                     QIVARVAGIAAGYEIEKILKEGGCKDSS"
     gene            complement(4011..>4287)
                     /locus_tag="VI935_05735"
     CDS             complement(4011..>4287)
                     /locus_tag="VI935_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25134.1"
                     /translation="DFIVGGRIGLNFYKKLTFEVRSDIGGFGLGSSSKIAWNIVSAIG
                     YELPWYRITPLIGYRALYDDFSTESGSNSFESKFWLHGPVLGIAFIF"
BASE COUNT         1312 a          778 c          857 g         1340 t
ORIGIN      
        1 tgaattcaat cataggtgta acttgctgtg cccagtttct cggcacacct cctcccagaa
       61 taagtatccc acgctttttg tatttcctat ctaagaagat ttctatatat ttttcattat
      121 cgagcagaag atcaacaacg accctttttt ttgggtgttt cttgttgaag gttgttaaag
      181 atattccgat ttcgctatca tgaatcgtgg gagaaaatat tgggatgccg ctttttaatg
      241 cgcttacaac aaagctctgt cctttatata aacctttccc caagatgtaa gtcgcaatcc
      301 ttttacagag ctctggagtg gagataatcc tacttccttc ttgtccccaa agctcattta
      361 gcaagaggtc aacttcattg agttttgttt catctaagag tgagtcgtac atcctgtcga
      421 tccgtaaatc tctaagctct aaatcgttca tcatatggtg tcctttgtga tgactaaatc
      481 ctaacgcttc taccatatcg tgcgcaattt gagagcctgt agtaattatg acgtcaataa
      541 attttagctc tatcaagtcg catataacac cttgaagtcc gccaggaaca ggggcgccag
      601 caattgttag ggtttttaaa caattaggtg ttttcaccat ctctaaaagg gtttttgctc
      661 cttctcctaa cgccctggcc tgaaaggaca tattagagaa tgcattaaca tagtctccta
      721 tgcattttag ctttctaacg gatggctgag ttactggaga gtgtaaataa tgttctcttt
      781 tcttcttcaa ataaacatct cccttcaatt agtcttaagc atcttaatgt atgcaggtat
      841 ttgagagggt ttatataaaa aatcttttat aataatcctc atggtccact gagctctgtt
      901 gttaatataa tcttaattta attcatttat aaaaatgatt gatcatattg aggttcatct
      961 aatcatgttt agagtttgta agtaggtatc atgtggatct tattctgtca agtattaagc
     1021 ttggtctcaa ggtgttgctt tacgacatta gttctaattg cttttactcc aactgcactt
     1081 cgggtgaaat ttgcttatca attacagagg tttttttatg caatacagtg tagtgggact
     1141 ggaaaacctt accttttgtt attttaggca aaagagcaga ggggtcgtta acgcgtctga
     1201 ttaagtgtag agacccattg caatgcgtct ctacgataat ttccgaataa attacatgta
     1261 gggctgatcc ccgtatcagt cgatatatta tgcctgttcg tggaacgggc aatacatcat
     1321 cctggaccct agcctgacgc caggacaggt ttatttcagg atctcctatc gtcatgcctg
     1381 aattaagtta tcgggcatcc atcgttttcc tggataccag ataactgagt acggagtgac
     1441 aatgcgcggt ggattcccaa taaagcttgt acccactaaa gacatgtggg taacatttcg
     1501 gaatgacaag cgcccattga gacctaaagg tttccgctct gctacatcct ataagattga
     1561 gcttatgcct tagttatgct atctttaaaa ggccgtgaag tgcccaaagt gtggatttaa
     1621 tagctttgaa taccttgatt cctgtaagaa atgcggtagt gctcttgggg taaacccaag
     1681 atacaaggtt atttacggat cttttgaaaa agataaagga caagaagatg taaatttagc
     1741 tgagagtaaa ataagcaaag aagagattgg gaaacctatc atttctactc cttctgtctt
     1801 aatttcatcc cctcaagaat ccttagaatc taaaaaaaga gaggaggtta taaaatctac
     1861 atttcctcct tctggctatt actcattacc tcaagagact ccaactttta ttgaagaagc
     1921 taaaagagga gaagaaatag ctgtatctga gagaaaggta agcaaagagg aaattgagaa
     1981 atctatcgtt tctacttcct ctgtcttagt cccaccctct gaagaatcct taaaatctaa
     2041 aaaaagtgac gaggaacttg taaaatcacc attatttact tccagctatt actcctcacc
     2101 tcaagagtct ccagcattcg cggagagcga agaattactt ccattcaatc ttgctggtat
     2161 gggcttaaga atcggagcct ttcttctaga tatcataata ttatttatgg taacgacgct
     2221 aacgctctgg atcgggtttt ttctaggtag tatcgttttt aacattagag aagagaaatt
     2281 cataaatgtt attgttcccc tatatctgat actttttttt cttagtacta catattttgt
     2341 ttttcttcag ggattttcag gaaaaacaat cgggaagatg ctcttcggta taagggtaat
     2401 cagaacggat ggagaatcta tagggttctg ggatgctcta attaggtggg ttggatattt
     2461 tatttcatta atatttatat ttattggatt tatctgggcc atttttgatc ctaagcgcca
     2521 agcttggcac gacaaatttg ctgagaccta tgttgttaaa gaataaccct gaatgagatt
     2581 tcttgacgaa attaattaca ggctaaatga aaaggctaaa gagattgagg actggattga
     2641 tgaacagagc cgaaaagcaa gggttccgct ttatacttca gtcgacctaa gggtctctca
     2701 tcataagatc acacctgttg atacaaatat attccccgca gggtttaaca acctctgccg
     2761 tgtttttaga gagcgtgtgg gaaggctttt tagagaagag ttcatacgga gtcatcctga
     2821 aacaagaaag attgttgtag tccctgagct tcataccagg aatccatact actgggaaaa
     2881 tatacaagta attaaaacta ttcttgagag tgagggattc ctcgttgaaa tcgggcttgt
     2941 aagtgacgaa ctctatcagg atggaatagg ttttaaagct ataagcggga aagaggtaaa
     3001 ggcttataag ctcaaaagaa aggacggtat tgtctacatc tcggaattta ttccagatct
     3061 aatcttaatt aataacgatt tctcctctga atgtcccgag attcttaggg atattagtca
     3121 gccagtcgaa ccccctgtgg aaatgggctg gcatacaaga agaaagaacg ttcactttga
     3181 gttctataat aaactcgccg gcgagcttgc agatataatt gagattgatc catggatgat
     3241 ttctatcgaa acattttctc aaaagggggt agacctcgat aaacccgagg atcgagaaat
     3301 tgtagctaca cacgtggatc ttcttttaaa tagacttagg aatgagtacg agaaaagggg
     3361 cgttaatgaa aagccctttg ttttcgtaaa gagtaactca ggcacatatg gtatgtcagt
     3421 attaagtgtt ttagccggag atgatattag aactcttaat gctgaaggaa gaaagcagat
     3481 gaaagtcact aagggtggaa agccagtaag ggatgttgtg attcaagagg gaatacctac
     3541 ttcccttaaa tttgaaccac aagttgtagg ggagccagtt atttatctga taaaatcgaa
     3601 tgtagcgggg ggttttctta gacaaaataa aggaaagagc aaatttgaca atctaaatac
     3661 gaagggaatg gaattttctt gcctctcttt tgagaatgag aattcttgtg agaacaagga
     3721 atgtgagaag tttttaccac ctgcttatca aatagttgcc cgagtagcag gcatagcggc
     3781 tggatatgag attgaaaaga ttcttaaaga aggcggatgt aaagattcct cctaaccgtt
     3841 tacatatttg atatgacagt gcttgcaaaa atttataatt tcataggatt ttgtttgcta
     3901 cgatacgtaa tttattagta gagccagttt ttaatctaaa tatatttaag ttacgaatcg
     3961 gacgttacta atgtcttaca aaataaaaga ttataaactc aatcatctct tcagaatata
     4021 aatgcaatcc caagcactgg accgtgcaac caaaatttgc tttcaaagct attacttcca
     4081 ctttcagtgg agaagtcatc gtatagtgct cggtatccga taagtggggt tattctgtac
     4141 cagggcaatt cataaccgat cgctgaaaca atattccatg caattttaga tgaagaacca
     4201 agcccaaaac ctcctatatc acttctaact tcaaaagtta gttttttata aaaattcaac
     4261 ccaatccgtc cgccaacaat aaaatca
//
LOCUS       DATDRL010000160         4809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2768413, whole genome shotgun sequence.
ACCESSION   DATDRL010000160 DATDRL010000000
VERSION     DATDRL010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2768413"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1292
                     /locus_tag="VI935_05740"
     CDS             45..1292
                     /locus_tag="VI935_05740"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HLE25135.1"
                     /translation="MNNIDGISTDKIVHEYGSPIFVVSAKTLKTNLRVFREEFSKKYP
                     QVLVAYSYKANYLPGILDIIHKEGAWAEVASGFEYDLARLLSVPGNSIVFNGPYKKKE
                     ELKKASKEGALINVDHLDELKQLEEIASELERTLEIGIRININVGVYQLPDRFGFNLD
                     SGEATQIVRRCVENNLLRIIGLHIHLTSYIIEPDHEENTIPARRINLIWPKSPNLYRT
                     AAEKIVRFANEIREKFAVNIRYIDMGGGFPSIELLDSYVDAIVYSILNGFRSELPILI
                     LEPGRAIVNNAVHLITTVVGVKEFPNDERGITVDAGINLLPTSFWRWQEIEPAAKVEG
                     DLRETTVYGPLCLQTDILAKAKLPELDAGNKLIIKNVGAYNISQSSSFIFPRPCVLLI
                     ENESVKVLRKAETADDIFKFETL"
     gene            1661..2893
                     /locus_tag="VI935_05745"
     CDS             1661..2893
                     /locus_tag="VI935_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25136.1"
                     /translation="MKSKRWVLLIFVFAILMLIEGLSYLSLFLLDKKFDVQYDPNVST
                     LSEEQKSHLRNFLNRKKGERAGQDPVLGWINRSEVNSAGMRDNIEYDKVPTAGKIRIS
                     AFGDSFTFGADVKLRDTWEKQLTLLDPSIEVLNYGAGAYGLDQAYLLYLRVGTEYSPN
                     IVFIGYMSENIARNVNVFREFYTSYYRDVIFTKPRFKVRDGDLVLIENPLSTLEDYKH
                     FLFNDKDVLARLGENDYHYQTNYNRGAFDFLQSVRFVKVFSHYLIIKVLNPIFNINGM
                     YNVNSEAYGVTVKIFDNFYHKVLENGSLPIILIFPDINDQRRSRDKKIRRYTPLITYF
                     RSKGYRFIDTLGALEPYESRYTVDELVENWGHYSPLGNRIIAEYIYLQLKNWDLLNLT
                     KLKEEIKVEYERLGTDIR"
     gene            3205..>4809
                     /locus_tag="VI935_05750"
     CDS             3205..>4809
                     /locus_tag="VI935_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25137.1"
                     /translation="MNHAIKLDPLQRVSLLVFLVLVISCSVGDTTPPAPIEDLSFDVM
                     TRQLNWTATGNDGNKGTATIYDLRFSTEPNVAEDFDNATHIEDLPRPFPAGDSQFFLL
                     PRLDVTGTEDFFFALDVRDEVGNNSGPSNVVEVTTSLVSIEFENNASGSCFAESIGSG
                     DFNGDFITDIIVGDPCLGMAYVFFGSTELIAEIIDVSIVDADVTIIGNPADSFAATVA
                     GIDSFGGGASDEIAIGAPGSNGETGEVFIIFGNPNLPPVIDCTIDCTQGDVSSRLITG
                     ENTGDEFGTSIETLVGVEGGSTIWVLIGAPGANSKTGEAYLFRANSIQELTSANEAEA
                     IFLGEAPGDMFGLTLTQAGNINDDSFSEFAVGAPGSGQVFVIFGSSDISVDTSIVIIS
                     NPADGFAFSISGGEDVDGDGLPDLLVGAPSTNMDTGSVFLYSGTAISMAQQSDTMPIY
                     ESEFTGESPDDMFGTSVSMLGDINPEVSEVKRTSFFVLELIPTDPDFAIGAPGTAEGG
                     SVYVFFGSDDGFPASVLASSCAMEASL"
BASE COUNT         1448 a          819 c         1024 g         1518 t
ORIGIN      
        1 ggcaaaaaga attctccagc agatgaaaga tatgcacttg gctgatgaac aatattgatg
       61 gcatttctac tgataagata gttcacgagt acggttcacc tatctttgtt gtttccgcga
      121 agacgcttaa aactaatctt agagtgtttc gagaagaatt ttctaaaaag tatccgcagg
      181 tattagttgc ttattcatac aaggccaact atctcccagg gattcttgat attatacata
      241 aagagggagc ttgggctgaa gtagcatcgg gttttgaata tgatttagca agattgctga
      301 gcgtgcctgg gaattcaatt gtttttaatg gtccgtataa aaagaaggag gaactcaaga
      361 aggcatctaa agaaggggcg ttgataaatg tagaccatct tgacgaactt aagcagcttg
      421 aagaaatcgc ttctgagctt gaaagaacac tcgagatagg aattagaatt aacattaatg
      481 ttggagtcta tcaattgcct gataggtttg gttttaatct agattctggt gaggcaactc
      541 aaattgtaag aagatgtgtc gagaataatc tccttagaat cataggactt cacattcacc
      601 ttacaagtta cattattgag ccagatcatg aagaaaacac cattcctgca agacgaataa
      661 acctcatctg gcctaagagc cctaacttat atagaacagc tgcggaaaag attgttcggt
      721 ttgcaaatga aatcagagaa aaatttgcag tcaatataag gtatatagat atgggaggtg
      781 gatttccatc tattgaatta ttggattcgt acgttgatgc cattgtttac tccattctta
      841 acggatttag gtcagagtta cctattctta ttttagagcc aggaagggca attgttaata
      901 acgcagtgca ccttattaca actgttgttg gtgtaaagga atttccaaac gacgagagag
      961 gaatcacagt tgatgctggc attaatcttc ttcctacctc tttctggagg tggcaggaaa
     1021 ttgaaccggc agcaaaagta gaaggtgatc tacgggaaac tactgtttat ggaccactat
     1081 gcttgcagac ggacatatta gcgaaagcaa aactcccaga acttgatgca ggaaataaac
     1141 tcattataaa aaacgttggg gcatataata tttctcaatc cagttcattt atattccccc
     1201 gtccttgtgt tcttcttata gaaaatgaat cagtaaaggt tttaagaaaa gctgagacgg
     1261 ccgatgatat atttaaattt gaaacgcttt aaagctgcta aggtttacac tcatctttca
     1321 aaaattgtca ttagtcatca acttaaatta agcctctcgg aatttaaaca ttaatttgat
     1381 aggttccttt tatagtaaag tattaaaatt cttttagcat ctataaaaaa gaaatatatc
     1441 tatataataa ataggtgtat catgcatttt ccgcttatcg tttattggta gtggattatt
     1501 attattttag actaaattgg ctcaaaaaaa tgcaaagcgt tgaattggta tattaaaaaa
     1561 ctttattcct attatttgat attaggtata aaataatcgt ttaaaattag ttgtagttta
     1621 gcttgtgata ttttatgagt attaaaaagt attgcttgat atgaaatcta aaagatgggt
     1681 attgttaata tttgtttttg cgattttgat gttaattgag ggtttatcct atctgagtct
     1741 atttttgctt gataaaaagt tcgatgtcca atacgatccc aatgtgtcaa ctctttctga
     1801 agaacagaag tctcatctaa gaaatttcct aaaccgtaaa aagggagaac gagcaggaca
     1861 agatcccgtt ttgggttgga ttaataggtc tgaagtaaac tcagcgggga tgagagataa
     1921 cattgagtat gataaagttc ccactgctgg taaaattcgt atctcagcct tcggcgactc
     1981 ttttacattc ggtgcggatg taaagcttcg cgatacatgg gaaaagcagt taactttact
     2041 agatccctca attgaagtgt tgaactacgg agcaggcgct tacggcctgg atcaagcata
     2101 cttactatac ctccgtgtag gaactgagta tagtccaaac atagttttta ttggatacat
     2161 gtctgagaac attgctcgta atgttaatgt gtttcgagaa ttttatacgt cgtattaccg
     2221 agatgtgata tttaccaaac cgcgctttaa agtgagggat ggtgatttgg ttctcattga
     2281 gaatcctctt tctactttag aggactataa gcattttctg tttaacgaca aggatgtact
     2341 agcaagactc ggagaaaacg attatcacta tcagacaaac tataacaggg gtgcattcga
     2401 ctttctacaa tctgtacgtt ttgtaaaggt attttcgcat tacctcataa taaaagtatt
     2461 aaatccaatt ttcaatatta atgggatgta taatgttaat tctgaagcct atggtgttac
     2521 agtgaagatt tttgataact tttatcataa ggtgctggag aatggttcat tgccaataat
     2581 ccttattttt cctgatatta atgatcagcg gcggagccgt gataagaaaa tacgtcgtta
     2641 cactcctcta ataacctatt ttcgctcgaa agggtatcgt ttcatcgata ctttaggagc
     2701 gttagaaccc tatgaatctc gttatacggt tgatgaactt gtagagaatt ggggtcacta
     2761 ctcacccctg gggaatagaa ttatagctga atatatatac ttgcagctaa aaaactggga
     2821 tcttttaaac ctgacaaaac tcaaagagga aattaaagta gagtatgagc gcttgggtac
     2881 ggacattcgt tgatacacct atactgtgcc cgaaactgta ctcgtagggg gtatagccga
     2941 gttacgcaag aattattaat aagttttcac aaggtcatac agcattgata cccatttttt
     3001 aaaattaggt catctctgta aatgacccac ttaattattt aacttcatac ttcaatataa
     3061 aaaacattca agaaaaatga atggctataa aagtgtttaa gaaattcgaa aaaatgattt
     3121 ccactcttta tttcttacta ttggaatatc cctattagtt cggtagaatt cataaacttt
     3181 aaaaatacga ccggagtcat tttcatgaat cacgcaataa aattagatcc cctccagagg
     3241 gtttcattat tagttttttt agttcttgtt atctcttgta gcgttggaga tacaactcct
     3301 cctgctccta ttgaggattt aagttttgac gtcatgacaa gacaacttaa ttggactgca
     3361 actgggaatg atggaaataa aggaactgca acaatttatg acctacgatt ttctacggaa
     3421 ccaaatgttg ccgaggattt cgacaacgcc actcatatcg aagacctgcc gagacccttt
     3481 ccggctggag attcacagtt ttttcttctt ccaaggctag atgtcacggg gaccgaagac
     3541 tttttctttg cgcttgacgt tcgcgatgag gttggtaata actcggggcc atcaaatgta
     3601 gttgaagtta caacgtcgtt agtttctatt gagttcgaga ataatgctag cggtagttgc
     3661 ttcgcggagt ccattggtag tggtgatttt aatggcgatt ttatcaccga tataatcgtt
     3721 ggggatccat gtttgggtat ggcttacgta ttctttggaa gcactgagct tatagctgaa
     3781 attattgatg tttctattgt tgatgctgac gtaactataa ttggtaatcc tgccgattcg
     3841 tttgctgcta cagttgcagg gattgatagt ttcggtggtg gggcctcaga tgaaatagct
     3901 ataggggcgc ctggttccaa tggagagaca ggtgaggttt ttattatatt tggtaatcct
     3961 aacctaccac ctgttataga ttgtactata gattgtactc agggtgatgt atccagtcgt
     4021 cttattacag gagaaaacac gggtgacgag tttggaacgt ctattgaaac cttagttggt
     4081 gtcgaagggg ggagtactat atgggtactt ataggtgcac ctggtgcaaa ctctaaaaca
     4141 ggcgaagcct atttgtttcg agctaacagt attcaagaac tcacatccgc taacgaggcc
     4201 gaagcaatat tcctcggcga ggccccagga gatatgttcg gtcttaccct tactcaagcg
     4261 ggaaatatta acgatgactc tttctctgaa ttcgcagtcg gtgcccctgg aagtggacaa
     4321 gtatttgtaa tttttggaag tagtgatatt agtgtagata catcaatagt tataataagc
     4381 aaccctgcag atggctttgc ttttagtatt tcaggtggag aggatgtaga tggagatggt
     4441 ttacccgacc tccttgtagg cgctcccagt actaacatgg atacaggttc tgtgtttctt
     4501 tactctggaa cagctatcag catggctcaa caaagtgata ccatgccgat ctatgaatcg
     4561 gaattcactg gtgaaagccc agatgatatg ttcggcacct ccgtaagcat gctcggcgat
     4621 ataaatccag aagttagcga ggtgaagagg acgtctttct ttgtattaga acttattcct
     4681 acagatcctg attttgcaat aggtgcacct ggaacagccg aaggagggtc ggtttatgtt
     4741 ttttttggaa gtgacgatgg ttttccggct tcagttcttg caagctcatg cgcgatggaa
     4801 gcctcgtta
//
LOCUS       DATDRL010000161         3669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3532198, whole genome shotgun sequence.
ACCESSION   DATDRL010000161 DATDRL010000000
VERSION     DATDRL010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3532198"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VI935_05755"
     CDS             <1..407
                     /locus_tag="VI935_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HLE25138.1"
                     /translation="ATELKVDEIVDLATLTGACVVALGDYTAGVMGNNERLINRIIKA
                     ARLAGEKVWQLPMDDELRKEIESDVADVKNVGSRFGGAITAAMFLEKFVGDTPWVHLD
                     IAGPAFIEKGGEWYPKGATGFGVRTIIRYLLS"
     gene            514..2160
                     /gene="sppA"
                     /locus_tag="VI935_05760"
     CDS             514..2160
                     /gene="sppA"
                     /locus_tag="VI935_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HLE25139.1"
                     /translation="MPLKIIDPIRRYNFVEIELHGEISEEEERFVIPFFPIRRKLTLW
                     DLEKIFLHASSAPSIKGIILKIRELHIGLARGEAIRRGILELREKGKRVFVYLEYPGN
                     IEYMIGSAGEIVFIPPWSLLNLIGLKAEVTFLKDTLDKIGIEAQIKGLGEYKSAAETF
                     TRKSMSEPHRNMIDSIMDDLYIQFVKRISKARGIDEEKTKSLIDSGPFTPEEALKFGL
                     VDGLGYESDFEKKINETLGYQLKKIQAKSFIRFINVKEIFRFIKEKIKRDAGIIALIS
                     DSGMVTLGESKGSGGTKTLGSQTLLKVLRKIEGDKNIKAIVLRISSPGGSGIASDIIS
                     NQLKILSEKKPVIVSMSDVAASGGYLIAIGARKIVAEPLTLTGSIGIISGKFIIKDFL
                     TKLGVTKEAVKRGTRALMFSSYKRFTEDEEERLNQIMKSFYQDFVKKVAMARGMDFIA
                     AEELARGRVWTGRQAKDNGLIDELGGIKESIQIAKKEAGIPDDISPFIKFFSQPKGIQ
                     FYPRDRNIIFGGKLDYLIENIKSLNNEAILTLMPFWININ"
     gene            complement(2234..3157)
                     /locus_tag="VI935_05765"
     CDS             complement(2234..3157)
                     /locus_tag="VI935_05765"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HLE25140.1"
                     /translation="MSSKPYKIWFDGKLVPWNEAKVHVLTHTLHYGLGVFEGVRAYEC
                     QDGRAAVFRLTDHINRLYSSARIAQIEIPFSKDEMRKAIFDLLVVNRFKEAYIRPIAF
                     IGDGAMGVHPRNNPIRVAIAAYPWGAYLGDEAVTKGIRAKISSYTRMHVNTFMTKAKI
                     SGNYVNSVMAKLEVTSLGFDEAVMLDTEGYVSEGSGENIFIIRNGTLKTPPLTSILEG
                     ITRNSTIEIAFDEGLHQVVTERFTRDELYIADEAFLTGTAAEITPIREVDGRMIGDGV
                     PGPVTRRLQERFFDVVRGKDEQFINWLDFVP"
     gene            3312..>3669
                     /locus_tag="VI935_05770"
     CDS             3312..>3669
                     /locus_tag="VI935_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HLE25141.1"
                     /translation="MPDQIIIKKYSNRRLYDTTHKKYVTLDEIANLIKDGSEIKVLDS
                     QTDEDITKIVLMQVVLESEKNKEDILPTSFLHMLIKYGNRMARDFFENYFTIMFQPYL
                     SIQEGMKKNLQLWQETG"
BASE COUNT         1209 a          623 c          766 g         1071 t
ORIGIN      
        1 atgctacgga actaaaagta gatgaaatag ttgacctggc aacactaact ggagcttgtg
       61 tcgttgcttt aggggattat acagcgggtg taatgggaaa taatgagcga ctgataaata
      121 ggattattaa agccgcaaga cttgcaggag aaaaggtatg gcaactaccc atggacgatg
      181 aactaagaaa agagatagaa agcgacgtag cagatgtaaa aaacgttggt agccgcttcg
      241 gtggggctat tacagctgca atgtttcttg agaaatttgt aggagacacc ccttgggtac
      301 atctcgatat cgctggtcca gcattcattg agaagggcgg tgaatggtat ccaaagggag
      361 ctacaggttt tggcgtaaga acaataatta gataccttct ttcgtgaatc ttggctcgtg
      421 gttcgtgact tcgcagtaag ctcagtcgaa cgtttcaata cacgatgtaa gatacacgat
      481 aaccgaaaca tagaccacgg gatagattag acaatgccac taaaaatcat tgaccctatt
      541 cgacgataca attttgttga gattgaactc cacggcgaga tctcagaaga agaggagagg
      601 tttgttatac cattttttcc aattaggaga aaactgacgc tttgggattt ggaaaagatt
      661 tttttacacg catcatctgc tccgtctatt aaagggatta tactaaaaat tagagagctt
      721 catatcggtc ttgctagagg agaggcaata agaaggggca ttttagagct cagagaaaaa
      781 ggaaaaaggg ttttcgttta tctcgaatac cctggaaata ttgagtatat gataggctcc
      841 gctggggaaa tagtatttat tcctccttgg tcattgttaa atcttattgg acttaaagcc
      901 gaggtaactt ttttaaaaga tactttggat aaaataggaa tagaggctca aatcaagggg
      961 ctcggtgaat ataaaagtgc tgctgaaacg tttacaagaa agtccatgtc tgagcctcat
     1021 agaaatatga tagattcaat tatggatgat ttgtatatac agtttgtaaa gcgtatttct
     1081 aaagcgaggg ggattgacga ggaaaaaact aagtctttga tagattcagg cccatttact
     1141 ccagaagagg ctctcaaatt tgggttagtt gacgggcttg gttatgaatc cgattttgaa
     1201 aaaaaaatca acgaaacact tggttatcaa ctaaagaaaa tacaagccaa gagcttcatc
     1261 agatttataa atgtcaagga aatttttcgc tttatcaagg agaaaattaa aagagatgct
     1321 gggataattg ctctaatctc agattctggc atggtaacgc ttggtgagag taaaggaagc
     1381 ggagggacaa aaacattagg ctctcaaaca ttattaaagg tgcttagaaa aatagaaggt
     1441 gataaaaaca taaaagctat agttttaaga atatctagtc caggtgggtc gggcattgca
     1501 tcagatataa tttcaaacca gttaaagata ttatcggaaa agaagccggt aatcgtttca
     1561 atgtctgatg tagcagcatc aggcggttat ctaatagcaa ttggggcaag aaagattgtt
     1621 gctgagccat tgacccttac tggttcaatt gggattattt cagggaaatt tattataaag
     1681 gatttcttaa caaaactagg cgtaactaaa gaagcggtaa agcgtggtac tagagcattg
     1741 atgttctcta gttataaaag gttcaccgag gatgaggaag aaagacttaa tcaaattatg
     1801 aaatcatttt atcaggattt tgtgaagaag gtggctatgg ctaggggaat ggattttata
     1861 gctgcggagg aattagcaag ggggagggta tggacaggaa gacaggcaaa agacaacggg
     1921 ctgattgatg agttaggcgg gataaaagaa tcaatacaaa ttgctaaaaa agaagcaggg
     1981 attcctgatg atatatctcc ttttattaag tttttttcgc aaccgaaagg aattcaattt
     2041 tatccccgag ataggaacat aatatttggt gggaaacttg attatcttat agaaaatata
     2101 aaatccttaa ataatgaggc aattcttact ttaatgcctt tttggattaa tattaactag
     2161 tgtattcaat ataatcccac attacatcaa aatatgtttg caatcttgta taaggttttg
     2221 ctagttaaaa taactaggga acaaagtcaa gccaatttat gaattgttca tccttccctc
     2281 ttactacatc gaagaatctt tcttgaagcc ttcttgtcac aggtccaggt actccgtcac
     2341 caatcattct tccatctacc tcccgaatag gagtgatttc tgctgctgtc ccggtgagaa
     2401 aggcttcatc ggcgatgtag agttcatccc tagtaaaacg ttccgttact acctgatgaa
     2461 gcccctcatc aaatgcaatc tctattgtgc tgtttcttgt aataccttct aggattgatg
     2521 taagaggtgg ggttttcaat gttccattcc taataataaa aatgttttct ccgctaccct
     2581 cagagacata tccttcagtg tcaagcataa ctgcttcatc aaatccaagg gaggtaacct
     2641 caagctttgc cattacagag tttacataat tacccgatat ttttgcttta gtcataaaag
     2701 tatttacgtg catcctcgta taagaagaaa tttttgccct tattcctttt gtcacggctt
     2761 catcaccgag atatgcaccc catggatatg cggcgattgc tactcgaata ggattattcc
     2821 ttggatgaac acccattgca ccatccccta taaaggcgat aggtctgata tatgcctcct
     2881 taaacctatt tacgacaaga agatcaaaga tagccttgcg catttcatct tttgagaagg
     2941 gtatctctat ttgggcgatt ctggctgagg aatatagcct attaatatga tcagtcagcc
     3001 taaataccgc agcacggccg tcttggcatt cataggctct gacaccttca aacactccta
     3061 atccatagtg tagagtatgt gtaagaacat ggaccttagc ttcattccac ggtactagct
     3121 tcccatcaaa ccagatctta tatggcttag aagacatcta aatctcccat aatacgttaa
     3181 tcacatttcc taataaagaa ttatagaatt ttccttattt tacagtaagt tgtcaagtat
     3241 acactggtat tattccttga ctaaatggtt ttttcatcaa ataatatatt agaaaaatta
     3301 aggagataaa tttgcctgac caaataatta taaaaaaata tagcaacaga aggctatatg
     3361 ataccacaca taagaaatat gtcacattgg atgaaatagc taaccttatt aaagatggta
     3421 gtgagattaa ggtcctagat tctcagacag acgaggatat tactaaaatc gtactaatgc
     3481 aggttgtttt agagagcgaa aagaataaag aagatatact acccacgtcc tttctacaca
     3541 tgcttatcaa atatggtaat agaatggctc gagatttttt tgaaaattat ttcacaataa
     3601 tgttccagcc ttacctttct attcaggagg gaatgaaaaa gaatcttcag ttgtggcagg
     3661 aaacagggt
//
LOCUS       DATDRL010000162         4291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2447636, whole genome shotgun sequence.
ACCESSION   DATDRL010000162 DATDRL010000000
VERSION     DATDRL010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2447636"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(402..548)
                     /locus_tag="VI935_05775"
     CDS             complement(402..548)
                     /locus_tag="VI935_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25142.1"
                     /translation="MINKNVLATIELTNNGWEIKNANVEKLVLVELLKVLTKELEEES
                     KRVA"
     gene            824..1288
                     /locus_tag="VI935_05780"
     CDS             824..1288
                     /locus_tag="VI935_05780"
                     /inference="COORDINATES: protein motif:HMM:NF015550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HLE25143.1"
                     /translation="MPKRRELSLKEKKFAIEYVKNKGNGTAAVKEVYDVKSNVNAAAQ
                     AYQKLLKPEIKEEITKILDDNGLDQQFIAQNLKQAIQSGIGERANNSDALRGIENLIK
                     LHNLNPISKSARLNVSLNSGYDSKNYDELLIELRKSRETTEKLLKDLGSEGG"
     gene            1461..1655
                     /locus_tag="VI935_05785"
     CDS             1461..1655
                     /locus_tag="VI935_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25144.1"
                     /translation="MRIKLKYILEDLIEEVDSIDWSDFEETIELERFYKKPRRYRILD
                     EENKVLTRLVISGLKFLATQ"
     gene            1655..1918
                     /locus_tag="VI935_05790"
     CDS             1655..1918
                     /locus_tag="VI935_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25145.1"
                     /translation="MKRIIVNVTDEQYEEIRKIAFGKRESMSSIVRGLLTTLEQATPI
                     VKKLMAEKIKTTYEELKNDSAKLNLCPHGKPKNLCKECIFKKK"
     gene            1934..2233
                     /locus_tag="VI935_05795"
     CDS             1934..2233
                     /locus_tag="VI935_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25146.1"
                     /translation="MWKGMKHLFFLVSVVILLALPLTVKGQGIETGGSDGPFDLCINV
                     DGVQEDYGSGSTELIQIDEEGNCALLEEVYPDIRERQDNPNFVEPGEEPKVSTCN"
     gene            2482..2970
                     /locus_tag="VI935_05800"
     CDS             2482..2970
                     /locus_tag="VI935_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25147.1"
                     /translation="MKEKKIGDLEEEIDAMGQESEIKASKWVKEKGKEWDKEAEVEKG
                     KALDVLEGKTRYKFADYKRFLGEELMRRGWEEFYPKDWMFHSTITDKGIVYYLRSPDK
                     RMFVRAFAPVNIPEYDFVAIEKILESAWECINNWEEEKNSRKSSIILPNGATKRLQNP
                     DN"
     gene            2933..>4291
                     /locus_tag="VI935_05805"
     CDS             2933..>4291
                     /locus_tag="VI935_05805"
                     /inference="COORDINATES: protein motif:HMM:NF036421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25148.1"
                     /translation="MELQNVYKTQIIERLKEERDTRDQILKEKASKDLYIFNKHILKA
                     EEGTEKVALAPFHKELCHFVQDNMHKKKLILVPRGHLKSTLITVGYSVFRLIQDPTVR
                     ILIQNAGYQTAADFVRAIKRHLQFNEDLIRIFGALAEDPEEWSENRITLKTAKSSEKG
                     KEPNITGWGVETTKTGQHYDLIIHDDLVERENIGTREQIEKVILRYKDSLDLLDPGGQ
                     MLVIGTRWTDGDLYSWIMDPENHVISSYEVMIRKALEWEGDLATALKSGEGIKSNLWP
                     EKFDNKELWTRYREKGPYEFSTQYLNDPVPPEDATFKREWFHYYDPTDVTGKLFHNYI
                     TVDPAISMERDADFTAMAVTSIDQYGNIFIREMVRAKLSPRQIIDQLFRLTEKWHPNR
                     IGVEDVAYQKALAYSIREEAAKRGRYLPIQEVRPGGRTKDQRIQALQPLYAAGKVFHW
                     KQM"
BASE COUNT         1381 a          800 c          943 g         1167 t
ORIGIN      
        1 taaatcctcc tcattatact tagcaccaca gtgttcacag actcgtgctc ttctgtctct
       61 tgcaattgct actctaattt caccatatat gtgtggcgga tccccagcca actcacattt
      121 acactcgtct attttttcta aacatcctct acaataagct aaactcatac gcaaacccca
      181 tttattatat ccgtacatat cggcccggat attccaaaca ttaaccaaag gaacaaggct
      241 acaatgatta tggttaatac ccaagcaaag acgtttagct ccttttgttt gtttaattgt
      301 ttagcaaaat cctgtttcat attattttat cccttaaaaa ctaaatactg gctagagatt
      361 gggtgggact tacagtcttg cgagtataag tccccgcaag actaagcaac ccttttgctt
      421 tcttcttcaa gttctttagt taacaccttg agtaactcca ctaacactaa tttttcgaca
      481 ttggcatttt tgatttccca accattgttg gtaagctcaa ttgttgctaa gacatttttg
      541 tttatcattt attcctttct actctaacca gtatttaact tttaaggtgc tactaaaaat
      601 cccgccggta agagcgggat caaagttatt gtatcacgct tacccgtgta tctgccatcg
      661 gtaccaaggc ttatcagtta cgggctgatt taagcttccc atctaaagca ccttggctcc
      721 ttccgatctg tagtgtgcta cgttctactg tctataatat agcatagtat aatagtcttg
      781 tcaagccccc agtttaaacc tagttttatg atataattaa aatatgccaa aaagaagaga
      841 actatcatta aaagaaaaga agtttgctat tgaatacgtc aagaataaag gcaatggaac
      901 agcggccgta aaagaagtgt atgatgttaa gtctaacgta aatgccgcag cccaagctta
      961 tcagaagcta ctgaaaccag agatcaagga ggagataacc aagatactag atgataatgg
     1021 actagaccag cagttcatag cccagaacct taagcaagct atacagagtg gtataggaga
     1081 aagagccaat aatagtgacg cattaagagg tatagagaat ctcataaagc tacacaactt
     1141 aaatccaatt agtaagagtg ctaggttgaa tgtctcatta aatagtggct atgactctaa
     1201 gaactatgat gaactactta tagaactaag gaagagtaga gaaactactg agaaactact
     1261 aaaggatcta ggatcagagg gaggctagta atagatcgag tataatggta ataacactcg
     1321 atgatgagcg tatatatccg acccctatac cacccctttt tattctcact agtaacgtag
     1381 aagaacctca ccaatacgga atatattttc aggtattaag acatcttgac atctagatat
     1441 taagatggta agatgtttgt atgcgaataa agttaaagta tatccttgag gacttaatcg
     1501 aagaagttga ttctatcgac tggtctgatt ttgaagaaac aattgagctg gaaaggtttt
     1561 acaaaaaacc ccgcaggtat aggatactcg atgaggagaa taaagtactt actcggttag
     1621 taatctcagg gttaaaattt ttagctaccc aataatgaaa aggataattg taaacgttac
     1681 cgacgagcaa tacgaggaaa taagaaaaat tgcatttggg aaaagggaat cgatgtcgtc
     1741 tattgtgaga ggacttctta ctactttgga acaagcaaca ccgattgtta aaaaactgat
     1801 ggcggaaaag ataaaaacta catacgaaga actgaaaaac gactcagcaa aattaaatct
     1861 ttgcccccac gggaaaccta agaatctttg taaagaatgt atatttaaga agaaatgaga
     1921 ggaggtgaac tttatgtgga agggaatgaa gcatttattc tttttagtta gtgtagttat
     1981 tttacttgcc ttgccgttga ctgtcaaggg acaggggata gagacgggag gttcagatgg
     2041 accttttgat ctttgtatca atgttgatgg cgttcaggaa gactatggta gcggcagtac
     2101 ggagttaatt cagatagatg aagagggtaa ctgtgctttg ttagaagagg tataccctga
     2161 cattagggag agacaggata atcccaattt cgtagagcct ggagaagagc caaaagtcag
     2221 tacttgcaat tagttgacaa ggggtgtatt attaaggtgt agttatggct gggatcgtcc
     2281 ttttctgggc gtggttatag ccagcctaac tacgccacgt tcagagaagg gcgattttct
     2341 tttctctcct ttctttacta ctactgaaag tagctccagt gggagatgac tagagagtag
     2401 tagttctttt tcaccgggac tttttctttt ctttctcttt tgtccttttc tctttcgttt
     2461 cttttttgtt atacttacta tgtgaaagaa aaaaagattg gtgaccttga agaagaaatt
     2521 gatgcaatgg gtcaggaaag tgaaataaaa gcgtctaagt gggttaaaga aaagggtaag
     2581 gagtgggaca aggaagcaga agtagagaag gggaaggctt tagatgtatt agagggaaag
     2641 actaggtata aatttgccga ctataaaaga tttttaggag aagaattgat gaggcggggc
     2701 tgggaggagt tttaccccaa ggactggatg ttccactcga ctattacaga taagggtatc
     2761 gtctattatt tgcgttcccc cgacaaaaga atgtttgtta gagcttttgc cccagttaat
     2821 attcctgaat atgactttgt agctatcgaa aagatcctgg aaagtgcctg ggagtgtata
     2881 aataactggg aagaggagaa aaacagtaga aaaagtagta taattttacc taatggagct
     2941 acaaaacgtc tacaaaaccc agataattga acgcctcaag gaagaaagag atactagaga
     3001 ccagatcctc aaggaaaagg ccagcaaaga tctatatatt ttcaacaaac atatcctaaa
     3061 agctgaggag ggcaccgaaa aagtggccct tgcccccttc cacaaggagt tgtgtcactt
     3121 cgtgcaggac aatatgcaca agaagaaact gattttagtc cccagaggcc atctcaagag
     3181 caccttgatc acagttggct actcggtatt tagacttatc caagatccaa ccgtcagaat
     3241 cctcatccaa aacgctggat accagactgc ggccgatttc gttagggcaa ttaagcgtca
     3301 tcttcaattt aacgaggatt tgataagaat attcggcgct ttggccgaag atccagagga
     3361 gtggagcgaa aaccgcatta ctttaaagac ggccaagagt tctgagaagg ggaaagagcc
     3421 caatataact ggttggggag tagaaaccac taaaacaggc caacactatg acctgattat
     3481 tcacgacgac cttgttgaaa gggaaaatat tggaactcgg gagcaaattg agaaagttat
     3541 ccttcgttac aaggattcgc ttgacctttt agacccaggt gggcagatgt tggttattgg
     3601 tacccgctgg acagatggag acctgtattc ttggataatg gaccctgaaa accacgttat
     3661 ctcctcctat gaagtgatga taagaaaagc acttgagtgg gaaggggatc tggctactgc
     3721 cttgaagagc ggggagggga taaaatccaa tctttggcca gagaaattcg ataataaaga
     3781 gctttggacc cgatatagag aaaaaggccc ctatgaattt tccacccaat acctaaatga
     3841 ccctgtaccg cccgaggatg ctacctttaa acgggaatgg ttccattact acgaccctac
     3901 tgacgtgact ggtaaactgt tccacaacta tataaccgtc gatccagcta tttctatgga
     3961 aagggatgct gattttacgg cgatggctgt tacttcaata gaccaatatg gtaatatatt
     4021 cataagggaa atggttcggg ccaaactatc ccctagacag ataatcgacc agttatttcg
     4081 gttgacggaa aaatggcacc ccaaccgaat tggcgttgaa gatgttgcgt accaaaaagc
     4141 tctagcctat tctataagag aggaggcggc taaaagaggg cgttacctgc cgatacaaga
     4201 agtgcggcca ggcggaagaa cgaaagatca acgcattcag gctttgcaac ccctttatgc
     4261 ggcgggcaag gttttccact ggaaacagat g
//
LOCUS       DATDRL010000163         3459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1330069, whole genome shotgun sequence.
ACCESSION   DATDRL010000163 DATDRL010000000
VERSION     DATDRL010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330069"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..163
                     /locus_tag="VI935_05810"
     CDS             8..163
                     /locus_tag="VI935_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HLE25149.1"
                     /translation="MLFDVESVFLFAWAVVFKRLGVLAFVEIIAFFIVILGGYFYVLK
                     KGALEWE"
     gene            154..639
                     /gene="nuoB"
                     /locus_tag="VI935_05815"
     CDS             154..639
                     /gene="nuoB"
                     /locus_tag="VI935_05815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HLE25150.1"
                     /translation="MGVNTNPVLGGDGFVTTKLSSFANWARKNSLWPMPFGTACCAIE
                     MMGAFASRFDLSRFGAEVVRFSPRQADLMIVSGTITYKMASVCRKIFDQMPEPKWVIA
                     MGSCTCGGGPFDSYAVVQGIDEFLPVDVYVGGCPPRPEALIDAVMKIQKKIDREGAPI
                     V"
     gene            636..1166
                     /locus_tag="VI935_05820"
     CDS             636..1166
                     /locus_tag="VI935_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HLE25151.1"
                     /translation="MSFDLQAVIDSLKSSLPDTILDIKTYRNETTLLIKKEKIQEFCL
                     YLKNNFDFKFIVDITAGDYLERKTPRYEVVYYLHRFGPEYSENVRIRLKVELPEDDTR
                     ILSVTSVWSGANWLEREVYDLFGIEFIGHPDLRRILMPEDYDAHPLRKDFDVRNREPS
                     KRSFERALKESDSGTS"
     gene            1201..2376
                     /gene="nuoD"
                     /locus_tag="VI935_05825"
     CDS             1201..2376
                     /gene="nuoD"
                     /locus_tag="VI935_05825"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HLE25152.1"
                     /translation="MRTETLTLNMGPQHPSTHGVLRVVLHLDGEIVVNAIPYLGYLHR
                     GIEKLCESITYQQCIPYTDRMDYVASICNNIGFILTVEKLLGIQDEIPERAKVAEVIL
                     FELGRIESHLIGIGTNALDLGAMSAFLYCFKERERIYDILETVCGARLTTSYPRVGGL
                     PDDLPENFEEAIQNFLKVFPRTLAEVDKLLTRNRIWVERTKGVAYISADDAIDLGLTG
                     PALRGSGIPYDVRKAIPYLGYENYDFEIPVSNDGDAYARYLCRLEEMHQSLLIIGQAI
                     NNLPDGPFIADLPEIVLPRKELVYTRMESLIRHFVLVYEGFKTPSGEVYHSVENPKGE
                     LGYYIVSDGSGKPYRIHVRGPSFVNLQALPKMVEGRLLADVIAAIGSIDIVLGEVDR"
     gene            complement(2704..3072)
                     /locus_tag="VI935_05830"
     CDS             complement(2704..3072)
                     /locus_tag="VI935_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25153.1"
                     /translation="MRYSSQKLFPYNRRFSRLLYVFPIILVSALSQVSFGQQGFDVSQ
                     AQYSFAIQIKNTIPGVIDAEWKSPFDLWVEADGVDQAAARNIVLDVIFAGKELLQQSF
                     CVHVHNGDFIQVAFQCWSYP"
     gene            complement(3107..>3459)
                     /locus_tag="VI935_05835"
     CDS             complement(3107..>3459)
                     /locus_tag="VI935_05835"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HLE25154.1"
                     /translation="DKVMDTPTTTTFLSGLGNIKEQFDAKFSSEKQFDDNGNYLIDLM
                     PKGKGEEYNMVTIAVDKKGMIVKDIFLYDPFGNLTKVSLKGIEINKGVPDSLFRFKVP
                     KGTEVIEAPSPVQQ"
BASE COUNT         1022 a          575 c          749 g         1113 t
ORIGIN      
        1 actttttata ctttttgatg ttgaatctgt ttttcttttt gcgtgggctg ttgtatttaa
       61 gaggcttggg gttcttgcat ttgttgagat tatagccttt tttatcgtta tcttaggagg
      121 ctacttttat gtattaaaaa agggggctct tgaatgggag taaatactaa ccctgtactt
      181 ggcggagatg gttttgtcac tactaagcta agctcatttg caaactgggc gaggaaaaac
      241 agcctctggc caatgccatt tgggactgca tgctgtgcaa ttgagatgat gggggcgttc
      301 gcatcgagat ttgatctttc aaggtttggt gcagaggtag tgagattttc accaagacaa
      361 gcagacctta tgatagtttc aggaactata acttataaga tggcctctgt atgtagaaag
      421 atttttgacc aaatgcctga accaaagtgg gttattgcta tgggatcttg tacttgtggt
      481 ggtggacctt tcgatagtta tgctgtagtt cagggtatcg acgaattcct tcctgtcgac
      541 gtttatgtag gaggatgccc tcctagacct gaagcgctta tagatgcagt tatgaaaata
      601 caaaagaaga ttgataggga aggggcacct attgtatgag ctttgattta caggctgtaa
      661 ttgattctct aaaatctagc cttccagata caattttaga tattaagacc tatagaaatg
      721 agaccactct tctaatcaag aaagaaaaaa tccaagaatt ctgtctgtac ctgaaaaata
      781 attttgattt taagtttata gtagatatta cagctggaga ttatctcgag agaaaaacgc
      841 ctcgttatga agttgtttat tatcttcata ggtttggacc agagtacagt gagaatgtaa
      901 gaatcaggtt aaaagttgag cttcctgaag acgatacgag aatattatct gtgacatctg
      961 tttggagtgg tgcgaattgg ctcgagcgag aggtttatga tctcttcggt atagaattca
     1021 taggacatcc tgatttaaga aggatcctca tgccggagga ttatgatgcg catcctctaa
     1081 ggaaagattt cgatgttaga aatcgcgagc cctccaagcg cagctttgaa agggctttga
     1141 aagagagtga cagtggaacg agttgagttt accactgata aagtaaatga actatcaggt
     1201 attcggactg agaccctcac gcttaatatg ggacctcaac atccttctac tcacggtgta
     1261 ctccgcgttg tgcttcacct agatggagaa atcgtggtaa atgcaattcc atatttgggc
     1321 tatctacata gggggattga aaaactctgt gaatccataa cctatcagca atgcattccg
     1381 tatacggata gaatggatta tgttgcatca atttgtaata acatcggatt cattctgaca
     1441 gtagaaaaac tccttggaat acaagatgag atccctgaaa gagctaaggt agctgaagta
     1501 attctatttg agttaggaag aatcgaatct catcttatcg gaattggaac taatgcatta
     1561 gacctaggtg caatgagtgc ctttctatat tgttttaagg agagagaaag gatctacgac
     1621 atactggaga cagtttgtgg ggcaaggttg actacctcat atcctagagt aggtggctta
     1681 cccgatgatc ttcctgagaa ttttgaagag gcgattcaaa atttcttaaa ggtgtttcca
     1741 aggacattgg cagaggtcga taagcttctt acaagaaacc gcatctgggt tgagaggacg
     1801 aagggtgttg cttatataag cgctgatgat gcaattgatt taggacttac tggaccagcc
     1861 ctgagaggaa gtggtattcc ctatgatgta agaaaagcga tcccctattt gggatatgaa
     1921 aactacgatt ttgaaatccc agtttctaat gatggtgatg cctatgctcg ttacctctgc
     1981 agattagagg aaatgcatca gagtctactt attattggac aagcaattaa taatcttcca
     2041 gatggcccat ttatagctga cctccctgag attgttcttc caagaaaaga acttgtatac
     2101 acaaggatgg agtctcttat aaggcacttt gtgttagttt atgaaggttt taaaacacca
     2161 tcaggagaag tttatcactc tgtagaaaac ccaaaggggg agttaggtta ctatattgtt
     2221 agcgatggtt ctggaaaacc atacagaata catgtgaggg ggccttcttt tgtaaatctg
     2281 caggcacttc ccaagatggt tgaaggaagg cttcttgccg atgttattgc agcaatcgga
     2341 agcatagaca tagtacttgg ggaagtagat aggtaaataa ttctatttta caatatctcg
     2401 aattttcttg ttaaatgctt ttggtttgta gatggaggct ggagataaat aattgttgat
     2461 taattatgat agctatttga ttattttgca aaattacatg ttctccatga gatttctaat
     2521 attatttgaa gtattacttt aagttactta tcgtaacttt aaatttatta agcttagtat
     2581 taacaattta cattagttct tatttgtaac taacagatta tgtgagtatt aggtggcggc
     2641 tattttaata atcctatagc taaatttcat cctggaaact tgagattcgt aggttgatgt
     2701 tttttaagga tatgaccagc attgaaacgc aacctgtata aaatcaccat tatgcacatg
     2761 gacacaaaaa ctttgttgca ataactcttt tcccgcaaag atgacatcaa gtacaatatt
     2821 tcttgctgct gcttgatcta cgccgtcagc ctcaacccaa aggtcaaaag gggatttcca
     2881 ctcagcatct atgacgccag gaatagtgtt ctttatttgg atagcaaaac tgtattgcgc
     2941 ttgtgaaaca tcaaaacctt gctgaccaaa actgacttga gaaagtgcac taactaatat
     3001 tatcgggaaa acgtatagga gtcgagaaaa tcgacgattg tatggaaata acttttgtga
     3061 tgaatatcgc attttatgtc ctcccttttt ggtcttagtt tatttattat tgctgaacag
     3121 gtgaaggggc ttctataacc tcagttcctt taggaacttt aaatctaaac aaagagtcag
     3181 gaaccccttt attaatttct atgcccttta atgatacctt tgttaaattt ccaaatgggt
     3241 catagaggaa gatatcttta acaatcatgc ctttcttgtc aacagctatt gtgaccatgt
     3301 tatattcttc accctttcct tttggcatta ggtcaatcaa gtagtttcca ttatcgtcaa
     3361 attgtttttc ggaggaaaac ttcgcatcaa attgttcttt tatgtttccg agtcctgaga
     3421 gaaatgtggt agttgtgggt gtatccataa ctttatcta
//
LOCUS       DATDRL010000164         2695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_631297, whole genome shotgun sequence.
ACCESSION   DATDRL010000164 DATDRL010000000
VERSION     DATDRL010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631297"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2177
                     /locus_tag="VI935_05840"
     CDS             <1..2177
                     /locus_tag="VI935_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE25155.1"
                     /translation="LLTFFPLIGSLGFEFSVTIAFIASFISVFISAEFVNLDFRERFI
                     RETRFADLVSSIFVLDFLLLAIPFVIGLLSSSIKDDCYIKEGAIFYVLIPVVTVFFSS
                     SLGLLIGNLFPKRGFLFGSLILIAIVCYSLWKLYNDPPIFSFNPVFGFFPGPLYDESI
                     PITSTLVVYTLITVCWGLFFLTILGVMRGIKFKVLKVWDLLALFVLVLILVITEIKEE
                     ELGISYNRKFIQDNILTGSMETEHFVIYFTPGTTEVNQISLIANDHEWRYHQLKEFLQ
                     VNSNDKIRSYIYPDNETRKRVIGAGETTIANPIHKEIHLVYDVFPAPLLKHELTHVMS
                     SEFGTRFLRISPKVGLIEGLAVASDWDGDGYTPHQWAEAMIKTNTAPDIREIIGIGFW
                     YAPPAKSYTTMGSFVRYLIDSYGIEKFKPLYRTGNFSDYGKGLDELVFEWKKFLDGIY
                     IPDKLLALARYKFSQPSIFQGRCPRRIAELADKGIKAYKDENYHEARGYFLDALNLNK
                     SDPVLNAGLAYAYYYNKNYNELNDLMNNSQTLPEVDKNILQNLRGNVLWQRGMVLEAK
                     SVFESLLGKPLPEDVKREIEIKLNAISSGNDIEKKIREYFSTREKLYQAAVLEEIIRD
                     FPDYSSSYYLLGKSYYSTGDYKLAASYLIESESLRLPSESLKMDNLRLLGISLFATGD
                     YEGAIKKFQFIVDLDTNGALKEYAIDFVERSEWARNRRWRNP"
     gene            2512..>2695
                     /locus_tag="VI935_05845"
     CDS             2512..>2695
                     /locus_tag="VI935_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HLE25156.1"
                     /translation="MKRNYIFHLPTSHDPKIASPLLIVFHGALMDGKGMRDATNLNDI
                     ADRRGFIVVYPDGYKRT"
BASE COUNT          782 a          491 c          563 g          859 t
ORIGIN      
        1 tattgctaac cttctttcca cttatcggat ctctgggatt tgagttctct gttacgatag
       61 catttatagc ttcctttatc tcagtcttta tctctgctga atttgtaaac cttgacttta
      121 gggagaggtt tataagagaa acaagatttg ctgatctagt atcgtctata tttgttctgg
      181 actttctttt acttgccatt cctttcgtta tcggactctt gagttctagc atcaaggacg
      241 actgttatat aaaagaggga gcgatcttct acgttctcat acctgttgtt acagtgtttt
      301 tctcttcgtc ccttggcctt ctcattggaa accttttccc aaaaagggga tttctatttg
      361 gctcgcttat tcttattgcg attgtttgtt actctctctg gaaactctac aatgacccgc
      421 cgatcttttc ttttaaccct gttttcggat tttttccggg tcccctttat gatgaatcga
      481 tccctataac ctctactctt gttgtctata cattgattac ggtgtgttgg ggtctttttt
      541 tcttaaccat tctgggggtt atgcgaggga ttaaatttaa agtattaaag gtgtgggact
      601 tgttagctct ttttgtattg gttcttatct tggttataac agaaataaaa gaagaggagt
      661 taggaattag ctataacaga aaatttatac aggataatat cttaacgggt tccatggaaa
      721 cagagcactt tgtaatttat ttcacacccg gcacaactga agttaatcaa atttcattaa
      781 ttgcgaacga ccatgaatgg agataccacc aattaaagga attccttcag gttaattcaa
      841 atgacaaaat ccgttcctat atctatcctg acaatgagac aagaaaaagg gttattgggg
      901 cgggggagac aacaattgcc aatccgatcc ataaagagat tcatctagtc tacgatgttt
      961 ttccggcccc tttgctaaaa catgaactca cccatgttat gtcctctgag tttggaacga
     1021 ggttcctaag aattagccct aaggttggat taattgaagg gcttgctgtc gcttctgact
     1081 gggatggaga tggttatacg ccgcaccagt gggctgaggc aatgattaaa accaacacag
     1141 ctcctgacat tagagaaatt attgggattg gattttggta tgcacctccg gctaaaagct
     1201 acacgactat gggttccttt gtccgttatt tgatagatag ttacggaatt gaaaaattta
     1261 aacccctcta tagaacaggg aacttttcgg attatggaaa gggtttagat gaattggtat
     1321 ttgagtggaa gaaattctta gatggaattt acattcctga taagcttctc gccttagctc
     1381 ggtataaatt cagccaaccg agtatttttc aaggtagatg tccaagaaga attgcggaat
     1441 tggctgataa aggtatcaaa gcatataagg atgagaacta ccatgaagca agagggtatt
     1501 ttcttgatgc cctaaatctt aacaaaagtg atcctgtgct taatgcgggt ctagcgtacg
     1561 cttactatta caataagaat tacaacgagc tcaacgacct gatgaacaat tcacaaacac
     1621 ttccagaggt tgataaaaac atccttcaga atcttcgagg taatgttcta tggcaaaggg
     1681 gtatggttct agaagcgaaa tctgtttttg agtcattact tggaaagccg ctcccggaag
     1741 atgtaaagag ggagatcgaa attaagctta atgccatttc aagtggcaat gatatagaaa
     1801 agaaaatccg agagtatttc agcactagag aaaagctcta tcaggcagcg gtgcttgagg
     1861 aaataattag ggattttcca gattactcct catcttatta cctgcttgga aaaagctatt
     1921 actctacagg ggattataaa ttggctgcct cttaccttat cgaatccgag tcattaagac
     1981 ttccttcaga gagcttaaag atggacaacc tgcgacttct tgggatttct ctttttgcaa
     2041 caggggatta cgaaggcgca ataaaaaagt ttcagtttat tgttgattta gatacaaatg
     2101 gggctctcaa agaatacgca attgattttg tggaaagatc tgaatgggca aggaatagaa
     2161 gatggagaaa tccgtagcat ttttgtggcg ggggagaatt actaggtgtc aattcaaagt
     2221 gaaaatttgg ccccttaggt gtaattgtaa aaggggtgcg tccccttttc cccgatttta
     2281 attgtgtaat actccctcaa gaacaggtgc tgatcttcaa taaagtgttc ctcattattt
     2341 tcatatgtta ttctaaaata tctttataac aatggcttta ttatgagatt tccttatatt
     2401 tttatattat taatttttgt gggattgacc ccggcttcag gatgttacag agccactaaa
     2461 tataaggaac ccagtaacag ggatcatgaa aggtcattac aattcggagg catgaaacga
     2521 aattatattt tccacctgcc tacatctcac gatccaaaaa tagcatctcc cctcctgatt
     2581 gtgttccacg gcgcattgat ggacggaaaa gggatgaggg atgctacaaa tttaaacgac
     2641 attgccgatc gaaggggttt catagttgtc taccctgatg gatataaaag gacct
//
LOCUS       DATDRL010000165         4637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2296956, whole genome shotgun sequence.
ACCESSION   DATDRL010000165 DATDRL010000000
VERSION     DATDRL010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2296956"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VI935_05850"
     CDS             complement(<1..287)
                     /locus_tag="VI935_05850"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HLE25157.1"
                     /translation="MKKLLSLLLSLIILAVIYWEIDLRSLGHVFKNCNGWWIVISLGI
                     LLPNILLAAGRLRQLLSNRAKLSFSNAYRLILAASTLNVILPMKIIGDLAKA"
     gene            complement(289..1878)
                     /locus_tag="VI935_05855"
     CDS             complement(289..1878)
                     /locus_tag="VI935_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015145976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25158.1"
                     /translation="MSKDFFSKEAISQIPKILILQDRNPHSPTYGCFDRNFWHYKIID
                     FPSGMAQEFVWPLALAYETNVINNPFYKKPAIKEWVEAGILFASRNAHTDGSCDDYFP
                     YERAVGAAAFSLLACVESYKLLDIKDETALQFFEKRGDWLANSQESGKLSNHQALIIL
                     CLELLSRLFNTTKWDRLREERLERLLSWQSPEGWFQEYEGCDPGYLTLTISLLARLYE
                     LRSAPKLKEALVRAVNFAWHFLHPDGSYGGEYGSRNTYNFFPHGFELIGRWMPEALSI
                     NDHFLIGLYKGLSPCYCDDHIIGHHTWNYLLAFRDFVVERSQPMPRPKGRTWFKEAKI
                     LIDRRDDTELYIALNKGGVFKFFRKNQLVASDTQFSLQVKNGGIKNAVGHLIDDYRIH
                     VGDNEIIIGGSLGWAKQKQMTTMNLLVLRLVMLSFGRFFPNLIRKLLQRMLITGKKEA
                     PFTFSRLLKWENGMWSVTDELRAKSWKKVITAGIGCNQTSIYVVMSRTFQIGQLQPWL
                     DLINEVKKLSPGQPLKLERNL"
     gene            complement(2090..3637)
                     /locus_tag="VI935_05860"
     CDS             complement(2090..3637)
                     /locus_tag="VI935_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009545474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25159.1"
                     /translation="MDRDPDSPTFGCFDRNYWHYKTCDFPSSILQQGVFTLEAIRNGS
                     VEFIAPTMVIERWCLGAVNALARQVDRKGSVNEYYPFERSYPAAAFSLYSICRILFDW
                     RSSAPQLLESVNWTPLRRLTNHVAGRIEKHAINQQAIGLAGLALAAKLDLISPLTEII
                     DKHATRLFNSQHSEGWFDEYGGPDSGYLTVTLDALADYYDATDDSRALEAIDRAVSFL
                     AKLVGADGRLPSTLNSRNTDYVVPYGLVRTAVHNTLAAWLVETLFHDIDQPAHPIWAT
                     DDRYHSHYICASVVRSLPYLEKMKPGQAPSLENSIWMDDCGFWIYWSSDRQWTAYVAA
                     RKGGLVRIHRAGGLAPITEYGWRIRAGSKLWTSNWWSAHWIVEKKPNVICIHGLCQRT
                     QFHIANVYKHIVLRLLAWMLREELIPLLKRMMIFRPAGASGPLFERVVEISEEGVCIK
                     DRIGTFDGAEAMPSPRQNLCHVASADSFSAEEWLPSLLGTGNRTLDEELFVNTRWNAK
                     KLSSINR"
     gene            complement(3745..>4637)
                     /locus_tag="VI935_05865"
     CDS             complement(3745..>4637)
                     /locus_tag="VI935_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015225481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HLE25160.1"
                     /translation="QGRLAFVGLPEQVVTLRMLQVAGHQAAQKVVFVAGPYTGTNMYF
                     GAVRAFLRAQGVPDHIAITSLQWRAGEWPGYLQVEISDGRIFRAKKFHYNYLIPFYIS
                     RNCQITPDFTNELTDLSVGDAWSPKFEKAGGGHSVIIVRSKKAQETLKDLQKSGDLAL
                     ESISFDQVLAMHSHMLDFKKRGAFIRLEAQRKRGLPVPDFGYKPEHIPQGRRLAEMII
                     SSCFALGRRHWARWFVSKLPLEFIGPIFDIIRKTWKTLSKPTKRRGLGDTTFIVTLND
                     SRWQELLLLSEGTNSKLNRI"
BASE COUNT         1291 a         1064 c          965 g         1317 t
ORIGIN      
        1 gctttggcaa gatctcctat tatcttcatc ggcaatatga catttagtgt gctagctgca
       61 agaattaaac ggtaagcatt actaaaacta agctttgctc tgtttgataa cagttgtcga
      121 agtcgcccag ctgcgagaag aatattcggt aatagtattc ctaggctgat gactatccac
      181 cacccattgc aattcttgaa tacatgtccg agactgcgaa gatcaatttc ccagtagatt
      241 actgccaaga taattaggct taaaagtaag gagagtagtt tcttcatctc aaaggtttcg
      301 ttctaatttc aatggttggc cgggtgagag ttttttgact tcgtttatta gatcaagcca
      361 aggctgcaat tgcccgattt gaaaagttcg actcatgaca acataaatcg aagtctggtt
      421 acagccgatg cctgcggtta tgactttctt ccaggacttt gctcgaagtt catctgtaac
      481 actccacatt ccattttccc acttcaacaa acgggaaaac gtaaatggcg cctccttttt
      541 gccggtgatc agcatccttt gcagcaactt ccggatgaga ttcggaaaga agcgtccgaa
      601 gcttagcata acgagtcgca ataccagcag gttcatggtc gtcatttgtt tctgttttgc
      661 ccagccaaga gatcccccga tgataatttc gttatcaccc acatgtattc tatagtcatc
      721 aataagatga cccactgcgt ttttgatccc accgtttttt acctgaagtg agaactgcgt
      781 gtcagaagcc accaactggt ttttacgaaa aaacttgaat acgccacctt tgttgagcgc
      841 aatgtatagt tcggtatcat ccctcctatc aatcagaatt ttggcttctt taaaccaggt
      901 gcggcccttt ggacgaggca ttggttgtga tctttctact acaaaatcgc ggaaggctaa
      961 taaatagttc caagtgtgat ggccgattat atggtcgtca cagtagcaag gcgaaaggcc
     1021 tttataaaga ccaataagaa aatggtcatt tatgctaagt gcctcaggca tccacctgcc
     1081 tataagctca aaaccatgag ggaagaagtt ataggtattg cggctcccat attcccctcc
     1141 gtatgaacca tcaggatgaa gaaagtgcca agcaaaattt acagctctaa caagtgcttc
     1201 ctttagtttt ggtgctgacc ttagttcata cagccttgct aatagtgaaa ttgttagtgt
     1261 aagatagcca ggatcgcaac cctcatattc ctgaaaccaa ccctctgggc tttgccaaga
     1321 taataggcgt tccagacgct cttctctaag tctatcccat tttgttgtgt taaagagccg
     1381 agagagtaat tcaagacaga gtattataag agcttgatga ttagagagtt ttccgctttc
     1441 ctgagagttt gcaagccagt ccccgcgctt ttcaaaaaat tgaagtgcag tttcatcttt
     1501 tatatctaat agtttgtagc tttctacaca agccaggagt gagaatgctg ctgcccctac
     1561 tgcacgttcg taaggaaaat aatcgtcaca agaaccatcc gtatgcgcat ttcgtgatgc
     1621 aaatagaatt cctgcttcaa cccattcttt aatagcgggc ttcttataga agggattatt
     1681 tattacattc gtttcgtatg caagtgctag gggccagaca aattcttgcg ccataccgct
     1741 tggaaaatcg atgattttat aatgccagaa attgcgatca aagcagccgt aagtcgggct
     1801 atgaggattg cggtcctgga ggatcaagat tttaggaatt tgcgagattg cctcttttga
     1861 aaaaaaatct ttactcattt aaaatgaaat ttaaacctta atgaaaagaa aattaaaata
     1921 attcaattct gaacgatcaa tccagtttat gaaagttatt tagttttttg tgaatcaaat
     1981 tcagaacggc gagtttgtag ttgaatgtca tcgagcatct tgcgatttac agccatcaaa
     2041 tccacaacca aaccaagcat ccaatccttt caaataattc ccactaattt tagcggttta
     2101 tagaggacag tttttttgca ttccatcttg tattcacaaa aagttcctca tccagcgtcc
     2161 ggttacctgt cccaaggaga gagggcagcc actcttcggc gctaaaactg tctgcgcttg
     2221 ccacatggca caagttttgt ctaggactag gcatcgcttc tgcaccatca aaggtgccta
     2281 tacggtcctt tatgcaaact ccttcctcac taatctccac aacgcgctca aataaagggc
     2341 cacttgcgcc tgccggtcga aaaatcatca ttcgcttcag taaagggatc agctcctctc
     2401 ttaacatcca agccaatagc cttagaacta tatgtttata aacatttgca atatgaaatt
     2461 gagttctttg acaaagccca tggatgcaga taacattagg ctttttttcg actatccagt
     2521 gtgctgacca ccaattgctt gtccacaatt tacttcctgc acggatgcgc catccgtatt
     2581 cagttatcgg ggctaggcct cccgctcggt gaattcgtac caacccgcct ttacgagcgg
     2641 cgacataagc tgtccattgt ctatctgatg accaatatat ccagaatcca caatcatcca
     2701 tccaaatcga gttctctaaa gacggagctt gcccgggttt cattttctca agatacggaa
     2761 ggctgcgtac cacgctggca cagatatagt gagaatggta tcgatcatcg gttgcccaaa
     2821 tagggtgagc aggttgatca atatcgtgaa ataatgtctc aacgagccaa gccgccaatg
     2881 tattgtgtac cgcagttctt actagaccgt atggtaccac gtagtctgtg ttgcggctgt
     2941 tcaatgtaga tggcagtcga ccatcggcac ccactaattt cgctaaaaag ctgacggctc
     3001 tatctatagc ttctaacgcg cggctgtcgt cagttgcatc ataataatca gcgagtgcat
     3061 ctagtgtaac tgttaaataa ccggaatccg gtccaccata ctcgtcgaac catccttctg
     3121 aatgctggga attaaacaag cgagttgcat gtttatcaat gatctcagtt agaggagata
     3181 tgaggtccag cttagcggcc aaggcgagtc ccgccaatcc tatagcctgt tggttaattg
     3241 catgtttctc aattcgtcct gctacatgat ttgtcaatcg cctcaacgga gtccagttaa
     3301 ctgattctag tagctgcgga gcggaagacc gccagtcaaa aagaattcgg caaattgaat
     3361 aaagactaaa tgcagcagca gggtagctac gttcaaatgg atagtactca ttaacactac
     3421 ccttccggtc tacctgtctt gctaatgcat ttacagcacc aagacaccag cgctctatga
     3481 ccatagtggg ggcgataaat tccacacttc cattgcgaat agcctccaat gtaaaaacac
     3541 cttgttggag aatagatgaa gggaaatcgc aagttttgta atgccagtag tttcggtcga
     3601 aacacccgaa ggtcggagag tctgggtctc gatccatctg cgttagcaca cgagcaagtt
     3661 gagctttggt gtaattctga aacttagctt tcagattaaa catgttgttg attttcgtga
     3721 atggtttcct atttgtttcg aaagttaaat tctgtttaat ttcgaattcg tgccttcaga
     3781 aaggagtaac aactcctgcc accgagaatc gttcagcgtc acaatgaagg tagtatcacc
     3841 taagcctcta cgtttagttg gcttagacaa cgtcttccaa gttttacgaa tgatgtcgaa
     3901 gataggtcca ataaattcaa gtggaagctt tgatacgaac catctagccc aatgacgacg
     3961 accaagagca aagcaactgc tgatgatcat ttctgctagt cggcgacctt gcggaatgtg
     4021 ttccggctta tagccaaagt ctggaactgg tagacctctt ttcctttgtg cttcgagtcg
     4081 aataaaggcg ccccgcttct taaaatccaa catatggcta tgcatggcta ggacctgatc
     4141 aaaactgatt gattcgagag caaggtcacc agatttctgc aaatctttca aggtttcttg
     4201 tgccttcttg ctacgcacta taatgactga atgtccaccc ccggccttct cgaatttagg
     4261 cgaccatgcg tctcccacag acaggtctgt aagttcgttg gtaaagtctg gtgtgatctg
     4321 acaatttcgc gaaatataaa agggaatcag atagttgtaa tggaattttt tagctctgaa
     4381 tatacgacca tcagatatct ccacttgtaa gtaccctggc cattcgccgg ctcgccattg
     4441 taaagacgta attgcaatat ggtctggtac accttgagct ctcaaaaaag cccgcaccgc
     4501 accaaaatac atgtttgttc ctgtgtatgg tccagccaca aaaactacct tctgggcagc
     4561 ttggtgccca gctacctgca acatacggag tgttaccacc tgctcgggta ggcccacaaa
     4621 ggctaagcgc ccttgaa
//
LOCUS       DATDRL010000166         3851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2663355, whole genome shotgun sequence.
ACCESSION   DATDRL010000166 DATDRL010000000
VERSION     DATDRL010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2663355"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..720)
                     /locus_tag="VI935_05870"
     CDS             complement(4..720)
                     /locus_tag="VI935_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HLE25161.1"
                     /translation="MKLNRNTEITYLGHATFKIKSPKGRTILVDPWVQGNPACPKEHQ
                     EFDKIDIMAITHGHFDHIGDAVTIGKEYKPKVVGIYETCVWVNSKGVQAIMPMNKGGT
                     QEVDGIKFTMVHADHSCGIQDDDGKIIYGGEAVGYVIEFENGFKIYHAGDTAVFSDMK
                     LIAEIYKPELAMIPIGDLFTMSPVEAARACRFLSPKYVIPMHYATFPILTGTPAQLRE
                     LTKDMKEMEIIELKPGETLT"
     gene            complement(978..1568)
                     /locus_tag="VI935_05875"
     CDS             complement(978..1568)
                     /locus_tag="VI935_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLE25162.1"
                     /translation="MDKKFFTMCAILSVLFLLFSFKISRTTEQKGKGLTKQDLIKRGE
                     YLVRFGGCNDCHTPKVLTPNGPVPDKTRLLSGYPSDSKMPTIDYSLVDTSKWVLFNYD
                     LTAAVGPWGVAFAANLTPDKQTGIGLWTEEIFTNSMRTGKHMGAGRPILPPMPWFNLE
                     HLEDKDLKAIFAYLQSLKPIKNLVPVPIPPSQLKTK"
     gene            1828..2682
                     /locus_tag="VI935_05880"
     CDS             1828..2682
                     /locus_tag="VI935_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25163.1"
                     /translation="MNGNKNCLFMIMGLLIFLFFSASQSFAIIPCSSVKIDLMSPETL
                     RSQNFNERLNWYKNKLSPCSQELKESADRNNIPTELLSTIILNELADINLLDLGQEWI
                     GVGKGSVGIAQIQIDTAIFHKLVDVTVEDIQKYQNSNEAVQKFGGKKPSRNETIRIIT
                     GKKLTEPGMAIEAAAREIRKILENIKACSSSSWTNNFLNGEIMLTNGNQSQIYDFIKG
                     GYLKEKRINLARMVAAPYNSTGIECVENPGNPFSPNDGGPFSNPRRRSELAAFIAEDL
                     FNGNIFNE"
     gene            3009..3422
                     /locus_tag="VI935_05885"
     CDS             3009..3422
                     /locus_tag="VI935_05885"
                     /inference="COORDINATES: protein motif:HMM:NF001617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HLE25164.1"
                     /translation="MDYEILDHTADACIRVYGKSFDELFENAARAMMELITDRERINP
                     SQEIEIEVRGETKEELLVHWLQEILFLHEVKKMVFKDFRLNLISETHAKGKAIGEKIN
                     IDKHELSFDIKAVTYHNLKIEPINDKLKVDIVFDV"
     gene            3605..>3851
                     /locus_tag="VI935_05890"
     CDS             3605..>3851
                     /locus_tag="VI935_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HLE25165.1"
                     /translation="MSEEIKLEKIHDYLWEIPKKGGMRVPGRVYATEMMLKDIQKDKA
                     LEQVVNVAYLPGIQKYSIAMPDIHWGYGFPIGGVAAMD"
BASE COUNT         1261 a          748 c          749 g         1093 t
ORIGIN      
        1 attctaggtc aacgtctccc caggttttaa ctctataatc tccatttcct tcatgtcctt
       61 tgtaagctct ctcaattgtg caggggttcc tgttaagata ggaaacgtgg catagtgcat
      121 aggaatcaca tacttaggtg aaagaaaacg gcaagcacga gctgcttcta caggagacat
      181 cgtaaataaa tctccaattg gaatcattgc caattctggc ttatagatct cggctatgag
      241 cttcatatcg ctaaaaacag cagtatctcc agcatgataa atcttgaatc cgttttcgaa
      301 ttctataaca taacctacgg cctcaccgcc ataaataatt tttccgtcgt cgtcttgaat
      361 cccacagcta tgatctgcat gaaccatcgt aaatttgatt ccatccacct cttgagtacc
      421 gcccttgttc ataggcatta tagcttgaac cccttttgaa ttaacccata cacacgtttc
      481 ataaatccca acgaccttag gtttatattc ctttccaata gtcacagcgt cgccaatatg
      541 gtcaaaatga ccgtgcgtga tcgccataat atctatcttg tcaaactctt gatgctcctt
      601 tggacacgca gggtttccct gaacccaagg atctactagt atcgttctac ccttaggtga
      661 cttaatctta aaagtagcat gccctagata tgttatttca gtatttcgat tgagtttcat
      721 ctctaaccct ccatttttgt ttattagttg ctgatgttaa gcactcaaat gttcaagtgg
      781 tacataaagc cgtcattccc gcatgttatc cgataaaagc tttcgtgtac aagatttagc
      841 gggaatccag cattaaatta ctttatggat gcccgatgaa agcattcggg catgacaaat
      901 tcacaagagt gcgtaacatt aatatgttaa attaaccaca atgacagaaa tattgcaacc
      961 tttataattt tatacaatca ttttgttttc aattgtgatg ggggaatagg aacaggcaca
     1021 aggtttttaa taggttttaa tgattgaaga taagcaaaaa ttgctttaag atccttatcc
     1081 tcaaggtgtt ccagattgaa ccaaggcatt gggggaagaa tcggacgacc tgcgcccata
     1141 tgcttgccgg tccgcatcga atttgtaaat atttcttctg tccatagacc gattcctgtt
     1201 tgcttgtcag gagtcaaatt cgcagcaaag gctacacccc aaggacccac agctgctgtc
     1261 aaatcgtaat taaaaagaac ccatttacta gtgtcaacta ggctgtaatc gatagtaggc
     1321 atctttgaat ccgaaggata gcctgacaaa aggcgcgtct tatcaggcac agggccattc
     1381 ggtgtcaata ccttcggtgt atgacaatca ttgcaccctc caaatcgtac gaggtattca
     1441 cctcgtttaa tcaaatcttg ttttgttaaa cccttccctt tttgttctgt ggtacggctt
     1501 atcttaaaag agaacaaaag aaacagcaca gacaagatag cacacattgt aaagaatttt
     1561 ttatccatag aatactcctt ttattttaag attatataaa tcttttttga gacataaact
     1621 aaaacaataa gttgggaata acaattttat atctacctgt aatcgagtaa atttacaaga
     1681 aaagctaaat tatgagcagt aatcggcaaa tttaagcaaa cttacaagag ccaatattag
     1741 tagctaaaga agaagactaa agatgctctt gaagagatgc aacaaagggc ttagtattaa
     1801 ttaaaactga tgtatataag atacaagatg aatggaaata aaaattgcct atttatgatt
     1861 atggggttat taatttttct tttctttagc gcttcacaat cctttgcaat aataccatgt
     1921 tcctcagtaa aaatagattt aatgagtccc gagaccctgc gctctcagaa cttcaacgaa
     1981 cgccttaact ggtataagaa taaactttct ccttgcagtc aggagttaaa agagagcgca
     2041 gatcgtaata atatccctac ggagcttctt agcacgataa tcttaaatga gcttgcagat
     2101 ataaatcttt tagacctagg tcaggaatgg attggggttg gaaagggttc agtaggaatt
     2161 gctcagattc agattgatac agctatcttc cataaacttg tagacgtgac agtggaagat
     2221 attcaaaaat atcaaaattc aaatgaagca gtccaaaaat tcggcggcaa gaagccatct
     2281 cggaatgaaa ctataagaat tatcaccggg aaaaaactca ctgagccagg gatggcgatc
     2341 gaggcggctg ctagagagat tagaaaaata ctagaaaata ttaaggcttg ttcttcaagt
     2401 tcttggacaa ataactttct caatggagaa atcatgctta caaatggaaa ccaaagccaa
     2461 atctatgact ttataaaggg tgggtattta aaggaaaaaa ggattaatct tgcacgaatg
     2521 gttgccgccc cctataactc cacaggaatt gagtgcgtag aaaatcccgg aaaccctttc
     2581 tctccaaacg acggcggccc cttttcaaac ccgagaagac gaagtgaatt agcagccttc
     2641 attgctgaag atttgtttaa cgggaatata tttaacgaat aggatttcta caactatttg
     2701 accatgtggg gcgaatccgc caatctgtag agacgcattg ctatgcgtct ctaaataatc
     2761 atatagtaat gcgggacaag aaaatcccta ttatcgttat cgtaacaatg ctaaatctat
     2821 cactcctcga ccccatcaca aaaccgatgc catgatagtc ggggttttca aaccccgacc
     2881 aacgggacaa gaatgtcccg tctatcggta tattttaaat tttgaataat aggggggaac
     2941 gtttgtgacc ctgccctcgg tgcattttaa atttcagaat cctcacattc atgctaaaat
     3001 cctaaattat ggactacgaa atactcgatc acacagcaga tgcgtgtatt agagtttatg
     3061 gaaaatcgtt tgacgaactt tttgaaaatg cagcgcgggc gatgatggag ttaataacag
     3121 atagagagag aataaaccct tctcaagaaa ttgaaatcga ggttcgtgga gaaactaaag
     3181 aggagcttct ggttcattgg cttcaggaga ttctttttct tcatgaagta aagaagatgg
     3241 tatttaagga ttttaggtta aatctgatta gtgaaactca tgcaaaagga aaagccattg
     3301 gtgaaaaaat taacatagac aaacacgaac taagctttga tattaaagcc gtcacttatc
     3361 acaatttgaa aatagaacct ataaatgata aattgaaagt agatatcgtg tttgatgtgt
     3421 aaatcatttt ctaacattat ctgccacgaa attaaggggt tgtacatcag acagtttggc
     3481 gaacccaatt gacacagata caggatgcaa gatgcaggat acaagataca agataaatat
     3541 aaaatctcgt gcatcatgaa tcttgcatct tgcatcattt caaaacaatg gggcgacgag
     3601 ggttatgagt gaagaaataa aactcgaaaa aatacacgat tatctttggg agatacccaa
     3661 aaaaggcggg atgcgtgtcc cgggaagagt ttatgctacc gaaatgatgt taaaagacat
     3721 tcaaaaagac aaggcgcttg aacaggtagt aaacgtcgct taccttccgg gaattcaaaa
     3781 atactccatc gctatgcccg atatacactg ggggtacggg tttccgatag gaggggttgc
     3841 cgctatggac g
//
LOCUS       DATDRL010000167         3952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2193469, whole genome shotgun sequence.
ACCESSION   DATDRL010000167 DATDRL010000000
VERSION     DATDRL010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2193469"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..306
                     /locus_tag="VI935_05895"
     CDS             121..306
                     /locus_tag="VI935_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLE25166.1"
                     /translation="MILEREENGSYHTFCPVLTGCHTQVDTYEVALENIKDAIRLYIE
                     SLIAHREKVPIKPALCS"
     gene            512..2095
                     /locus_tag="VI935_05900"
     CDS             512..2095
                     /locus_tag="VI935_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLE25167.1"
                     /translation="MPTHFVYFLFNKSNVIKPCYIIYTIESLRRKNMRQIGLRVEILF
                     NILFLTAVAMFLIGIIAFKVTERFALQGKIDGARSIIAAFESLYAKDGDLEGRFDLLK
                     DFLEPGAWMVISDRQTRRVFHVDSTKEVKNIADPLILEVMKTGRPKVNFVGINMPPFA
                     FYKGFMIASPLRRDGKQEGVVFLYQPLLSLEKGIFSGQRLIAISIILDLIVIALFGFY
                     LLSKRVIRPVHELIETTGEIARGKFPSKTNLGGVKEIVQLNDALRKMYDEIELNKMKL
                     KENIKALEESNQALLTTQKELIASEKLASLGKLSAGIAHEIGNPLSAIKGYVELLKKG
                     YILQDDNRAESLSSIEKELVRIDRIIRTLLDYSRPRGFALKRVGVNDVIKDAAELVKS
                     QGLLKNIDLKLDLATGLSLIEIDPYQLSQVLINLILNARDAILRDGVITISSLGTPNE
                     GVEISVTDNGVGIPKEIIDKIFDPFFTTKDPGKGTGLGLSVSQRIVQHFGGRIRVQSE
                     PEIGSVFRITFPGVKDHGS"
     gene            2085..3449
                     /locus_tag="VI935_05905"
     CDS             2085..3449
                     /locus_tag="VI935_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLE25168.1"
                     /translation="MAAKVLIIDDEEGVRKTLSLNLGMEGYEVESVGGADTGLNLIDS
                     GKNYDFIISDIRMPGMDGIQFLKEIKDRDLGSIVIMISAYGTVETSIEAIKCGASDYV
                     NKPINTDELFLRMRMAEERERLRRENLLLRKGLGKDDGFEAIVFTSDKMGGVVDFVKR
                     VSEYKTTVLITGESGTGKELIAHAIHARSPRKDGAFVAINCSAIPESLLESELFGYVK
                     GAFSGASQSKRGLFEEANGGTLFLDEIGEFPIHLQSKLLRVLQEEEIRRLGDTKTIKV
                     DVRIIAATSRDLNEDVKQGGFRGDLFYRLDVLPIHIPPLRERKEDISTLVDYFIKKYN
                     EKLNRFVKGVSQQAMSELLQYPWYGNVRELENVVERAMILCDSNSIDRVNFTLKDKNI
                     QGDAWFDSLSLDKAWQKLEKAYIEKALLASGGNRTQAAKLLGLSRRSLLYKLKQYGYS
                     EEED"
     gene            3570..>3952
                     /locus_tag="VI935_05910"
     CDS             3570..>3952
                     /locus_tag="VI935_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25169.1"
                     /translation="MSATAQLPKYWIKNPATDLTFFDFGWVLVFLPLILLKEHIAFII
                     LVVLIVNYIHRHYTFALVYGDKEEFEKRKNIYVFLPIVAVIVTFISIYFDGFKILVLI
                     SVLWTMYHTITQKYGITRIYSRKAG"
BASE COUNT         1238 a          635 c          903 g         1176 t
ORIGIN      
        1 tattaaaaac tatgtcttgt tgcttgatga taacttattg gtctaataga taatcattta
       61 ttctaaaggt taaaggctaa tgttctgagg tgagaagaag taaaagaata caattacaca
      121 ataattctcg aaagagaaga aaacggaagt tatcacacct tttgtcctgt tcttacagga
      181 tgtcataccc aagttgatac atatgaagta gctttagaaa acatcaaaga cgctataaga
      241 ctctacatag agagtcttat agcccatcga gagaaagttc ctatcaagcc tgctttatgt
      301 tcatagagga ttgatagtat aattgtatgg gattctgttg gattcctgta ttgaggtcta
      361 ttgatttttg acaaactaat tcgggaataa acactgggta gtccatcggg atctgtggta
      421 gctatccaga gggaggttct tttagggaac cacatgcttg ctagtggacg gcaacgagca
      481 agcctgtcct gagttgcaac gaagggaccc gttgcctacc cactttgttt atttcttgtt
      541 taataaatca aacgtcataa aaccttgcta tatcatctat actatagaga gtttaagaag
      601 aaaaaacatg cggcagatag ggctaagggt tgagatactt tttaatattc tttttcttac
      661 cgctgttgcc atgttcctta ttggtatcat cgcttttaag gttactgaaa gatttgctct
      721 ccagggaaag attgacgggg ctaggtctat aattgctgca tttgagtctt tatacgccaa
      781 ggatggagat ctagagggca ggttcgattt gttaaaggat tttcttgagc caggagcatg
      841 gatggtgatt tcagacaggc aaacaagaag ggtgtttcat gtagattcaa ctaaggaggt
      901 taaaaatata gcggatcctc tcattcttga ggtaatgaaa actgggagac ctaaggttaa
      961 ttttgtagga atcaacatgc ctccgtttgc gttctataag ggatttatga ttgcctctcc
     1021 gcttagaaga gacggaaaac aggaaggtgt ggttttcctg tatcaacctt tactttcact
     1081 tgaaaaaggt attttctcgg gtcagagact gatagctatt tcgattatat tggatttaat
     1141 tgttattgcg ctttttgggt tttacttact ctcaaaaaga gtgattagac cagttcatga
     1201 actcatagaa acaacaggag agatagcaag gggtaagttt ccatctaaga caaatttggg
     1261 tggggtgaaa gaaatagttc aactcaacga cgccttaagg aagatgtatg atgaaattga
     1321 gttaaataag atgaagctca aagagaatat aaaagcccta gaggaatcta atcaggcact
     1381 tctaactacg cagaaagagc ttatagcttc tgaaaagctt gcatcccttg gaaaactttc
     1441 agcgggtatt gcacatgaaa tagggaatcc tctaagtgcc ataaagggtt atgttgagtt
     1501 attaaaaaag gggtacatac tgcaggatga taatagggct gagtctctga gctctatcga
     1561 aaaagagctg gtgaggatag acagaattat ccgcacccta ctcgattact cccgaccaag
     1621 aggttttgca ttgaagaggg ttggcgtaaa tgatgtcatc aaagatgctg ctgaattagt
     1681 taaaagtcag gggcttctaa aaaacattga tctaaagctc gatcttgcta ctggactcag
     1741 tcttatcgag atagatccgt atcaactatc ccaggttctt ataaacttga tactaaatgc
     1801 tagggatgct atccttcgag acggggtgat tacaatatcc tctttaggca ctccaaatga
     1861 gggtgttgaa atttctgtta cggacaatgg cgttggaatt ccaaaagaaa taattgataa
     1921 gatatttgat ccatttttta ctacaaagga tcctggaaag gggacaggac tcggcctttc
     1981 agtgtctcaa agaatagttc aacactttgg cggaaggatc agagtgcaaa gtgagcctga
     2041 aattggttct gttttcagaa taacatttcc tggtgttaaa gatcatggca gctaaggttc
     2101 tgataattga tgatgaagag ggtgtaagaa aaacgctttc gcttaatcta ggaatggagg
     2161 gttacgaggt tgaaagcgtg gggggcgcag acactggcct caatcttatt gatagtggta
     2221 agaattatga ttttataatc tcggatatac gtatgcccgg tatggatgga attcaatttc
     2281 taaaggagat taaagatcgt gacttggggt caattgtaat aatgatctct gcctatggga
     2341 cagtagaaac atcaattgaa gctataaaat gcggtgcctc cgattatgta aataaaccta
     2401 ttaatactga tgagctcttt ctacgcatgc gtatggctga agagagagag aggcttagaa
     2461 gagaaaacct actactcagg aagggactcg gaaaggatga tggattcgag gcaatagtct
     2521 ttacaagcga taagatggga ggggtcgtag attttgtaaa aagggtttct gaatataaga
     2581 ctaccgttct tataactgga gaaagcggaa cgggtaaaga gctaattgcg catgctattc
     2641 atgcaagaag ccctagaaag gacggggctt ttgtagcaat aaactgttct gcgattcctg
     2701 aatcactcct agaaagtgag ctatttgggt atgtaaaggg tgcttttagc ggagcaagcc
     2761 aatctaagag aggacttttt gaagaggcta atggcgggac tttatttctg gatgagattg
     2821 gagaatttcc tattcatctt caatcaaaac tccttagggt tcttcaggag gaggagataa
     2881 gacgtcttgg agatacaaaa acgataaaag tagatgttcg aatcattgcg gcgacctcac
     2941 gtgatctcaa tgaggatgtt aaacaaggag gcttcagggg cgacttattc tataggctag
     3001 atgttcttcc gattcatatt ccaccgctca gggaaaggaa ggaggatatt tcaaccttag
     3061 ttgattattt tataaaaaaa tacaatgaga aattgaatcg ttttgttaaa ggtgtgagtc
     3121 agcaagcaat gtcggagctt cttcagtatc catggtatgg aaatgttaga gagcttgaaa
     3181 acgtagtaga aagggctatg attctttgcg attcgaattc aatcgataga gttaatttca
     3241 ctttaaaaga taagaacatt caaggtgatg cttggtttga ttccctctcg ctcgataaag
     3301 cctggcagaa actcgaaaag gcgtatatag aaaaagcgct tttagcaagt ggcggaaatc
     3361 gcacacaggc tgcaaaactc cttggcctta gtcgccgatc tttgctttat aaactcaagc
     3421 agtatggtta ttccgaggaa gaggattaac atttctaagc aagctaaggg gttcaaaaga
     3481 acaatgtttt attgacagta gacgtttttg tatgatatat gtaaaataac cattttttca
     3541 aaaaaccagc aagtaataca taagttttaa tgagcgcaac tgcacagtta ccgaaatact
     3601 ggataaaaaa tcctgcaaca gatttgacct tttttgactt cggttgggtt ctggtttttc
     3661 tacctcttat tttattgaag gaacatattg ccttcataat tcttgtggta cttatagtaa
     3721 attacataca tagacactat acctttgcac tcgtatatgg tgataaagaa gagtttgaaa
     3781 agaggaaaaa tatctatgtg tttctcccca ttgttgcagt tattgtgacg tttatttcta
     3841 tatactttga tggttttaag atacttgttt taatctcggt tctttggact atgtaccata
     3901 caattacaca aaaatacgga attacgagga tctattcccg aaaggctggc ta
//
LOCUS       DATDRL010000168         2444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3495572, whole genome shotgun sequence.
ACCESSION   DATDRL010000168 DATDRL010000000
VERSION     DATDRL010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3495572"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /gene="dcd"
                     /locus_tag="VI935_05915"
     CDS             complement(<1..471)
                     /gene="dcd"
                     /locus_tag="VI935_05915"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011364835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HLE25170.1"
                     /translation="MSILTRNEILGEIKQGNIKIEPFSTEYLGPGSLDLHLSNEFRIF
                     KNMRRVIEIRDDARYEDVTEKITVNDHLILMPGEMVLGITREKITLSHNICGWLEGRS
                     RFARLGLLVHISTSFIQPGISNKQCLEIINFSPMPLALYPGTAICQFIFQRTIGE"
     gene            complement(960..1556)
                     /gene="leuD"
                     /locus_tag="VI935_05920"
     CDS             complement(960..1556)
                     /gene="leuD"
                     /locus_tag="VI935_05920"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HLE25171.1"
                     /translation="MEPFKTLTGIVAPLDRVNVDTDQIIPKQFLKRIERTGFEEYLFY
                     DWRYLDNGKPNPEFVLNNERYKDATILLTRNNFGCGSSREHAPWALRDYGFCVIIAPS
                     FADIFFSNCFKNGMLPIVLPESVVEELFKRVFANIGYRLTVDLGSQIITDDKGFKIRF
                     EIDPFKKKCLLEGLDDISLTLKNEAKIRAYEERRGLVN"
     gene            complement(1571..>2444)
                     /locus_tag="VI935_05925"
     CDS             complement(1571..>2444)
                     /locus_tag="VI935_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HLE25172.1"
                     /translation="DIILSIIGHIGTAGATGTVVEYRGDAIEALSMEERMTICNMSIE
                     AGARAGMIAPDEKTFDYLEGRPYAPKGKDFERSLELWKQLKTDDGAIFDKRVLLDAST
                     MAPQVSWGTSPGMVTDVTGIVPHPDSFADANDVKAARQALEYMGLIPGTPIQEIKVDR
                     VFIGSCTNSRLEDLTAAARVVKGKKATPHVRAMVVPGSQSVKREAEKLGIDKIFKEAG
                     FEWRESGCSMCLGMNPDILAPGERCASTSNRNFEGRQGKGGRTHLVSPIMAAAAAIEG
                     HFVDIREWRLDEEK"
BASE COUNT          716 a          548 c          384 g          796 t
ORIGIN      
        1 ttctccgatg gttctctgaa atataaactg acatatagct gttccaggat agagagcaag
       61 cggcatagga ctaaaattta ttatctctag gcactgcttg ttgcttatgc ctggctgtat
      121 aaaactagta gatatgtgta ctagcagacc tagccttgca aatctgcttc taccctcaag
      181 ccatccgcag atattatggg ataaagttat tttttctctt gtaatcccaa gtaccatctc
      241 tccaggcatt aaaattaaat gatcgtttac tgtaattttc tctgtaacat cctcatatct
      301 tgcatcatct cttatctcaa tgaccctccg catgttttta aaaatcctga attcgttact
      361 aagatgaaga tcgagtgagc ctggacctaa atattcggta gagaatggtt cgattttgat
      421 attaccctgt tttatctcac ctaaaatttc atttcttgtt aaaatactca ttctatctcc
      481 ttcaattaaa agttcttaat gaatttagtc taacgtgata atctaaaatc acaatccaag
      541 taagcttcaa catattggta tggccttatt gatcttgttt agtatgtatc ttcgtaatga
      601 gtcaatatgt aattgggatt attataaaat caaattaact catatcttca tgtattatct
      661 tcaaaatttt actcactatc tgagtaatat ttaattgttt atgaagagct gtggataagt
      721 tatttaaagc tctcaaaaag ctttaacatt tgtggaaaag acaattttaa tattcttaag
      781 taatggttaa tatatgattc ataagtagct gttattccta aataaaaatc ccattatgga
      841 tttaatttta gtgtagagac gacccgccgt atcttctcta catacattat tatctgaaaa
      901 cctttgagct aaaaatagta agcttggaaa tgtgtaaatt tcggttgaag ttataactgt
      961 taattaacta aaccgcgacg ctcctcataa gcccgaattt tagcttcatt ctttagtgta
     1021 agacttatat catcgagtcc ttcgaggaga cactttttct taaaagggtc tatttcaaat
     1081 ctaatcttaa agcctttgtc gtctgttata atttgagaac ctaaatcaac tgtaagacga
     1141 taacctatat tagcaaaaac ccttttgaaa agttcttcta caacactctc aggtaaaaca
     1201 ataggaagca tcccattttt aaaacagttg ctaaaaaata tatcagcaaa cgatggagca
     1261 ataattacac aaaatccata atctcttaat gcccatggtg catgctcacg agagctcccg
     1321 catccaaaat tatttcgagt gaggagtatc gtggcgtcct tatatctttc gttattcaat
     1381 acaaattcag gatttggctt tccattgtct agataccgcc agtcataaaa aagatattcc
     1441 tcaaagcctg tccgctcaat tcgctttaaa aactgctttg gaattatttg atccgtatcc
     1501 acatttaccc tatccaacgg ggcaacaatt cctgtcaaag ttttaaaagg ctccatatct
     1561 tctactcctg ctatttctct tcatcaagtc tccactcacg aatatcaacg aaatgacctt
     1621 ctatcgcagc ggcggctgcc atgataggac ttacaagatg agttcttccg ccctttccct
     1681 gacgaccctc gaaattccta tttgatgtgc ttgcacatcg ctcgccgggt gcaagtatat
     1741 ccggattcat acctaggcac atactgcatc cagattcccg ccattcaaat cctgcctctt
     1801 taaaaatctt atcaatacct agtttctcag cctctcgctt taccgattga gaacctggta
     1861 caaccattgc gcgtacatgg ggagttgcct tcttaccttt caccacacgc gcggcagcag
     1921 ttaaatcctc aaggcgagag ttcgtgcatg aaccaataaa aaccctatca actttaatct
     1981 cttgaatggg cgttcctggt ataagcccca tatattctag agcctgtctt gcggctttaa
     2041 catcatttgc gtcggcaaaa gaatctggat gaggaacaat cccagttaca tctgtaacca
     2101 tgcctggact cgttccccag ctcacctgag gggccatcgt agatgcatcc agaagaaccc
     2161 tcttatcaaa tatcgctcca tcatcagttt tcaattgctt ccacaattct agagaccttt
     2221 cgaaatcctt tccttttggt gcgtatggcc ttccttctaa ataatcaaag gttttttcat
     2281 ctggagcaat cattcctgct ctggctccag cttcaatgga catattacag atcgtcattc
     2341 tttcttccat cgagagagcc tcgattgcat cacctcgata ttcaaccacc gtacctgttg
     2401 cacctgctgt tccaatatga ccaattatgc tcaaaataat atcc
//
LOCUS       DATDRL010000169        11758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1864461, whole genome shotgun sequence.
ACCESSION   DATDRL010000169 DATDRL010000000
VERSION     DATDRL010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11758
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864461"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VI935_05930"
     CDS             <1..648
                     /locus_tag="VI935_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25173.1"
                     /translation="KRTWASGGMIFGAGREGVDDVGFVTTLIDNLSKELNIDLKRVYA
                     AGLSNGGMFAHRLGCELSDRIAAIASVGGTMPTDISKSCKPKRPISVMIIHGTKDRLV
                     RWEGGSRKEQTNGFLSVPATVNKWVEIDGCSAPPKHTVLADKFDDGTRIRREAYEKCK
                     DKAEVILYVIEGGGHLWPNGFGKSKFLELLFGNISEEIDVGEVIWDFFQRHSITN"
     gene            complement(791..1303)
                     /locus_tag="VI935_05935"
     CDS             complement(791..1303)
                     /locus_tag="VI935_05935"
                     /inference="COORDINATES: protein motif:HMM:NF019224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HLE25174.1"
                     /translation="MQSFHPENPTCGTGPTTVFVTSQTFAGDLGGIVGADQICNNLAQ
                     NAGLPGTYIAWLSTSTVNARDRLNSNGPYRLVIGTIIADDLQDLLDGFLDAPINIDEN
                     GTLILGNQEVWTGTLADGSAALGRCSDWTANSNSVFGTAGNATSTAQSWTVFVNLNCS
                     MFNRLYCFQQ"
     gene            complement(1408..1584)
                     /locus_tag="VI935_05940"
     CDS             complement(1408..1584)
                     /locus_tag="VI935_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25175.1"
                     /translation="MRRYLSSLIVALVFATMVVIAGTPPAKSGATSGDGDCLEFCKDQ
                     AICACAKILGGVYV"
     gene            1855..2394
                     /gene="lspA"
                     /locus_tag="VI935_05945"
     CDS             1855..2394
                     /gene="lspA"
                     /locus_tag="VI935_05945"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HLE25176.1"
                     /translation="MNKYKTVVSISLVVVFLDQITKMLIRASLALHETVPIFPFLDIT
                     HLRNRGAAFGIMNDLPETIRMPLFVLILVAAFIAILTFVIKAESGDRILVTSLSLILG
                     GAIGNSIDRFRLGYVTDFIDFHWFGDPKYHWPPFNVADAAITIGVILILLQALFLKKV
                     SYTATKAQRHKGTKKEDKF"
     gene            2520..3152
                     /locus_tag="VI935_05950"
     CDS             2520..3152
                     /locus_tag="VI935_05950"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HLE25177.1"
                     /translation="MFTGIVEDVGRVEELKPKTKEVLFTIGAKNIDDKEIKIGESISV
                     NGACLTVISVKKNIFTVEASHETLRRTTLSKLKVGSKVNLERSLRLGDRLGGHIVNGH
                     VDGMGKVESIERRGKSLEFWFSIPKVHSKYIVEKGSIAIDGVSLTINALNVDRFSVNI
                     IPYTQEATIFSNLKPGDSVNIEFDIIGKYVEKFVMGKSGRKDFSQLLERF"
     gene            3495..4025
                     /locus_tag="VI935_05955"
     CDS             3495..4025
                     /locus_tag="VI935_05955"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HLE25178.1"
                     /translation="MTRNKTPKNIFLIGFMGAGKTTVGKILAKRLGLNFIDLDEVIEK
                     ELNLTIREIFSRYGEDFFRDAETKALRSIADKDRYVIAAGGGVVLRKKNWQIMKPNGF
                     TVYLRAPAEVLWTRVRNNASRPLLQVENPFDRLCELLSQRISQYEKADLIVDTENVSP
                     EHLALEIAEKMKNLLT"
     gene            4065..5114
                     /locus_tag="VI935_05960"
     CDS             4065..5114
                     /locus_tag="VI935_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HLE25179.1"
                     /translation="MPKKSRGATRRLKKVTPVPLMQMISGFWASKTLASAVELDLFTK
                     LSGRHADVHQLSQLLGIHPRPAEMLLSACTALGLLGKRDKRFSNSSLSEEYLVKGKPC
                     YFGGVVTLLDRRLYLPWNRLLEALRTNRAQTWEDHSDIFEQISSNLDKQRLFTEGMHS
                     FSVQSGKALAAAFDFSQYTQLLDVGGASGAYCIEAVRRYLDLRAVVFDLPTALEITQE
                     KIAESGFSDRIITCPGNFFTEELPKGSDVILLSMILHDWDPERNLMILHKCFNALPSG
                     GTVIISELMMDDNKAGPVSAALMSLNMLIETEAGRNYSWAEYTEWLEKTGFKKIERIA
                     MESPGANGILVGYKS"
     gene            5258..5983
                     /locus_tag="VI935_05965"
     CDS             5258..5983
                     /locus_tag="VI935_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLE25180.1"
                     /translation="MAKVNEIAPDAYRISIYVPEFNLQFNHFLIKDDEPLLFHTGYKR
                     MFPQVRDAVATIIKPSEIRWISFSHFESDECGSLNQWLEVAPSAQAACSVVGALVSVN
                     DFAIRPAKGMADDEMLTTGKYRFRFCSTAHLPHGWDAGVLFEEANRTLLCSDLFHHDG
                     DVEPLTESDIIERVRKTLIEYQASPFANYIPYTPQTDQILQRLAALKPKTLATMHGSS
                     FVGDGERALLDLALVIKETLGSP"
     gene            6104..6595
                     /locus_tag="VI935_05970"
     CDS             6104..6595
                     /locus_tag="VI935_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HLE25181.1"
                     /translation="MNREKNVSGFTLQPGIGKSLWVLGDLYTFKVVGEETGGAIAVLE
                     VQVQPQNGPPPHIHHREDETFYVLEGEFSFLHGDTTFTATAGSFVYIPKGTVHTFKNV
                     GTSRGRFLVIITPAGFEKFFEKIGEPVTDKTSPPPFNPETVKKLLALAPNFHLEVKLP
                     PDS"
     gene            6718..7725
                     /locus_tag="VI935_05975"
     CDS             6718..7725
                     /locus_tag="VI935_05975"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HLE25182.1"
                     /translation="MKIIVIFIIALCYHFACSNERDLNKIEGEIKQDKLRSQDFTKDP
                     TKFFAHRGLLNFAPENTLLAFEIAKERGFKNIELDIVTTKDNKIVVSHDFNLKRMFKI
                     NRDVCKVNYDELLELKPSQYFFEAVLGLPEEKIIAIRSTDGPWIKRLDSQQISLLDDV
                     FKTFGKDFNYHLDIKIIGCEQELIQELVSVIEKYDLENNVFIESTRLKLLEKIISTNP
                     KIKTIYWNDEIFSFSDEEWQDLVEKGIDSVDLRYSNINKKEVLRIKSQIKNIRIHTYT
                     VNSLKVINSLDNFVDYIITDLDIFGNNVDKYRFFNQKSQKIKLSSPLYQKFKSILSYF
                     K"
     gene            complement(7837..9786)
                     /locus_tag="VI935_05980"
     CDS             complement(7837..9786)
                     /locus_tag="VI935_05980"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HLE25183.1"
                     /translation="MDLSKLYQIKNSSYSVWLLEIGPPIVVIASFWCKIVYFYYSLEY
                     VWWGWNEPFNKWIEAHPNLYTANLASILPLFSLVIFLPRISRLVVLLLLDFLLTSLII
                     ADKLHVLWFSEIISIPEISSVFMLPKVSESIIKLLKPSDLGYYLDIIWGILLLPFYSK
                     ACKQIQPLDWKYIKRISAGSLVASILIAAPTLRLVWNDKYALFTPNTIQRDACGTIGL
                     LSYHIVDALSHIGGWHEKRGEVNLERVKNFLGSQHNERNSRSNLFGIAKGRNVIMIMV
                     ESLNSFPIGLEVNGQPITPHLTAFSKESLYFINFYDQAHLGSTSDGEFISLQSLYPVA
                     RGTVSMNYHSRSFFGLPAILSQHGFTTLSATAEAASFWNMERMHKKLGFQQSYFQDKY
                     NITETINGWLSDYEFFIQTIPILQARREPFLAYLITHSTHHPYMVPKKYHELNLGEME
                     GSMIGNYLHSVHYFDKVFGTFVNGLRKSGLLDRSIVVVYGDHKSKHIELEYIKKLLGY
                     PESSEYRTWMLLQRLPLIIRLPYGEEAGMRKVTGGHLDIAPTVLALLGVIDEKNVMLG
                     SDLTNGENSMVVFRNGSFADGKYIFINRFRQTSQSACYEASTGRITDCKPLEEKRLKA
                     LERLEISDLIIHGDLIPALRANKNN"
     gene            10128..11594
                     /locus_tag="VI935_05985"
     CDS             10128..11594
                     /locus_tag="VI935_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HLE25184.1"
                     /translation="MKRLSTLLVLILSVFVITMIGCEKLEEGTKPQKKTDTEKTTEKK
                     EDILETPRVQELPSFADLVEKLKPSVLNISTTSTFSQRGLFPKSPFGEENPFEEFFKR
                     FFGDIPQQEFKQSGLGSGFIISEDGYVVTNNHVIDKAQDIEVVLEDGKKYKADIIGKD
                     AKTDLAVLKINPEKMLQAVVFGNSDKLRIGDWVVAIGNPFGLGYTVTVGIVSAKGRSL
                     GLSSYDDFIQTDASLNPGNSGGPLFNQMGDVIGVNTAIVAGGQGIGFAIPINMAKNVI
                     EQLKDKGKVVRGCLGVLVQPITPAIAESMKLKEPKGALVGDVTPNSPAEKAGIQRGDV
                     IVDFNGNKIEDVSDLTTLAAVTPPGTEVKLKVMKDGSVKDIDVKLGEFPDEVAESTEK
                     EVKEKLGLTVHEITPSIAARFRLETDKGVVITHVDQGSVAQESGLQPGDVILDVDGQA
                     VNNIDDYKNAIDKMEKGKSALFLIKRGKNTIYVGIRTE"
BASE COUNT         3648 a         2141 c         2571 g         3398 t
ORIGIN      
        1 aaaaggacct gggcctcagg aggcatgatt ttcggtgccg ggcgcgaggg tgtagatgac
       61 gttggttttg tcactaccct cattgataat ctatcaaagg aactcaatat tgatttgaaa
      121 agagtctatg cggcgggatt atcgaacggc gggatgttcg cccatcgtct aggctgtgag
      181 ctatctgaca ggattgcggc aattgcatca gttggaggca caatgccaac tgatatatct
      241 aagagttgta aacccaaaag accaatatcg gtgatgatca ttcatgggac gaaggatagg
      301 ttagtccgat gggagggcgg gagtaggaaa gagcaaacaa acgggttcct ttctgtgcct
      361 gccactgtca acaaatgggt cgaaatagat ggctgctccg ctcctccaaa acatacagtc
      421 ctggctgata aattcgatga cggaactcga atacgcagag aggcttacga aaagtgtaaa
      481 gacaaagctg aagtaatcct atatgtcatt gagggaggcg gccatttgtg gccgaatggg
      541 tttggaaaga gcaaatttct ggaattattg tttggtaata taagcgaaga aatcgatgtc
      601 ggtgaagtga tttgggattt ctttcagaga catagtataa cgaattaaga aatgccggcg
      661 cgtgcggtcg ggttgctagt gcgacctccg cgcgtgggtt gggtgtgccg gtttggcggc
      721 ggggcgggct gagggtcacc ggagaggcaa gctatgtgtt gcgatagggg acgcactcct
      781 gtacggggag tcattgctga aagcagtaga gacggttgaa catactgcaa tttaggttta
      841 caaagacggt ccacgactgg gcagtagagg tggcgttacc ggctgttcca aaaaccgaat
      901 tgctattggc cgtccagtca gaacatctac ccagggctgc actgccgtca gccaaggttc
      961 ctgtccatac ttcttgattt cctagtatga gtgtaccgtt ctcgtctata ttgattggag
     1021 catcgaggaa cccgtcgagc agatcctgca aatcatctgc tattatagtt ccaataacga
     1081 gcctgtacgg accgtttgaa tttaagcggt ccctcgcatt aacagttgag gtagacaacc
     1141 atgctatata tgttcctggt agacctgcgt tttgggcgag gttattacat atctggtctg
     1201 cccctacgat tcctccgagg tcgcccgcaa aggtttgact cgtaacgaat acagttgttg
     1261 gtccggttcc acaagtgggg ttttcggggt gaaacgattg cacgacaacg tccttttccc
     1321 acagcgcaca tataggatcg acatcgcatt cacactggct cccgggatta ccagtgtcaa
     1381 cacactgggc aagaatgtta cactttgtta gacgtaaact cccccgagta ttttagcgca
     1441 agcacaaata gcctgatcct tacaaaactc aagacaatca ccgtctccgc tcgttgctcc
     1501 actctttgct gggggcgtgc cggcaattac aaccattgta gcaaatacta atgcgactat
     1561 taaagaacta aggtaccttc tcatcctctg atagctcctt ttcatcagat ttaattcatt
     1621 ttgtagcaca tatcatatcc aaagtcaaca gaaaagtatt tttgttttgg attgtaagca
     1681 cgaatttatt cgtgcaaatg ttattgcaca gattatgtcg caccaggatg gtgcttctaa
     1741 ggtattgtta ttgtaagagc agcttctaac tgcgattaga tcacgccaag cttgccttgg
     1801 gatttttctt tgttgaagta acaagtttat ccgctatact cttagctcta gaaaatgaat
     1861 aaatataaga cagtagttag catttctctt gtcgttgtat ttcttgatca aataacaaaa
     1921 atgcttatcc gagcgagcct tgctctccat gaaaccgttc cgatattccc ctttctcgat
     1981 ataacacatc ttagaaatag gggagctgct tttggaataa tgaatgatct tcctgaaacg
     2041 attcgtatgc ctctttttgt attaattcta gttgcggctt ttatagcaat cttgactttt
     2101 gtaataaagg cggaatccgg ggataggatt ttagtcactt ctctttctct tattctgggt
     2161 ggagctattg ggaattctat agatagattt cgacttggat acgttacgga ttttattgac
     2221 ttccactggt ttggagatcc taaatatcac tggcctcctt ttaacgttgc tgatgcggca
     2281 atcacaatcg gtgttatatt aatcctattg caggctttat tcttaaaaaa agttagctat
     2341 acagccacta aggcacaaag gcacaaaggc acaaagaagg aagataagtt ttaacttaga
     2401 agtcaggagt cagaagaacg gaggccttac atccttagtt tattctggat cccggcttct
     2461 ggattctaaa ttccaacttc gtgtctttgt ggctaaacag gtaagaaggt taaataagga
     2521 tgtttacagg aatagtcgag gatgtcggaa gggtcgaaga actaaagccc aagaccaaag
     2581 aggttctttt tacaatcgga gcaaagaata tagatgataa agaaattaag atcggtgaga
     2641 gtatttcggt taatggggca tgtcttacgg tgatctccgt caaaaaaaat atattcacag
     2701 ttgaggcttc tcatgaaacc ttgagaagga caaccctttc aaaactaaag gttggaagca
     2761 aggtaaacct ggaaagatct ctaagattag gggacagact tggcgggcac attgtaaacg
     2821 gtcatgtaga tggaatggga aaagtggaat ccattgagag gcgtggaaaa tcgcttgaat
     2881 tctggttttc aatacctaaa gtgcattcta agtatattgt ggaaaagggt tctatagcaa
     2941 tagatggagt gagtcttact ataaatgctt tgaatgtaga tagattttct gtaaatataa
     3001 tcccatatac tcaggaagcc actatattta gcaatctaaa gccaggtgat tccgtaaata
     3061 tagaattcga tataatagga aagtacgtag agaaatttgt aatggggaag agtgggagga
     3121 aagatttttc tcaacttctt gaaaggtttt gataattatt caaccactta tttccgaata
     3181 tcaaatgttt ggaattcaaa cgctgataat ccgataccat tttcaatata aatattgtag
     3241 acacgaacta gctcatacaa agtaggattg taggcacgaa cttgttcgtg caattactat
     3301 ggcacaggca ggtctgtgcc tacaaaaaga ttgcagattg taggcacgaa tttaatcgtg
     3361 caaatggtat agcttaacta gttaataatg ggaaatagta ctacatttga caatttagaa
     3421 atttaaaatt gtatagaatt tgtgatttat gatttgaaat ccaacttctc atttatggct
     3481 aagtagaaaa agttgtgact aggaacaaaa cacctaaaaa catctttctt ataggcttta
     3541 tgggtgcagg gaagacaact gttgggaaaa tacttgctaa aaggcttgga ctaaatttca
     3601 ttgacctaga cgaggtaatt gagaaggaac tcaacctgac tattcgagag atattctcaa
     3661 gatatggaga ggattttttt agggatgctg aaacaaaagc gctccggtct atagctgaca
     3721 aagatagata tgtaattgca gcaggtggtg gtgtagtttt aagaaagaaa aactggcaaa
     3781 ttatgaaacc aaacggattt actgtatatc ttagagcacc agccgaagtt ctttggactc
     3841 gtgtaaggaa caatgcatca cgtccgcttc ttcaggttga aaatccattt gataggcttt
     3901 gtgagcttct ttctcaaaga atctcccaat atgaaaaagc cgatttaatt gttgatactg
     3961 aaaatgtctc tcctgaacat ttagcattag agatagcaga aaagatgaaa aatcttttaa
     4021 cttaacaaga ttgaagaaac caagtgttaa aaaggagcaa atctatgccc aaaaaatctc
     4081 gtggtgcaac aagaagatta aagaaggtaa cacctgttcc cttaatgcaa atgatctcag
     4141 gtttctgggc ttctaaaaca ctcgcatcag cggtagaact agacctcttt actaagctat
     4201 caggtagaca tgccgatgtt caccaactat cgcaattgct aggaattcat ccaagacctg
     4261 cagagatgct tcttagtgcc tgtacagcct taggcttgct tgggaagcgg gataaacgtt
     4321 tctctaactc ctccctatca gaggagtatt tagtgaaagg taaaccttgc tattttggtg
     4381 gcgttgttac acttttggat cggcggttat atttgccttg gaaccggctt ttagaggctc
     4441 taagaaccaa cagggcgcag acttgggaag atcattcaga tatttttgaa cagatctcct
     4501 caaatctaga taaacagcgc cttttcacag agggtatgca tagctttagt gtgcagtctg
     4561 gaaaggccct cgcggcggcg tttgactttt cccaatatac tcagcttctc gatgtgggcg
     4621 gagcttcagg tgcgtactgt attgaagcag tacgtcgcta tctggatctc cgtgcagtgg
     4681 tctttgacct tccgacggca ctggagatta cacaagagaa gatagccgag tcaggatttt
     4741 ctgatcggat tataacctgt ccgggtaact ttttcactga agaactaccg aaagggtctg
     4801 atgttattct cctttcgatg attctgcatg actgggatcc tgagaggaac ctcatgattc
     4861 ttcataagtg ctttaacgca ttaccgtcag gtggtacggt gataattagt gagttaatga
     4921 tggatgataa taaggcaggg cctgtgtcgg cggctctaat gtccttaaat atgcttattg
     4981 agacggaggc aggccgtaat tatagttggg ctgaatatac tgagtggctt gaaaaaactg
     5041 ggttcaagaa aatcgagcga atagctatgg aatctccagg agccaacgga attctagtcg
     5101 gctacaagtc ttaaattaga caatagtatg ctagttaaag atgcacgatg ctgtcagaac
     5161 tttaatacat catgcctcgt gcatcctgta acatgtatct tgtcattcgt gtaaattagt
     5221 ttttatcggt aaaatattta tgaaaggaga aacggctatg gcaaaggtta atgaaattgc
     5281 tcccgatgcg taccggatct cgatctacgt acctgagttt aatctacagt tcaatcactt
     5341 tctcatcaag gatgatgagc ccctgctatt ccacactggc tacaagagaa tgttcccaca
     5401 ggtgcgcgat gctgtggcaa ctatcatcaa gccgtcggag atacgctgga ttagcttcag
     5461 ccattttgag tcggatgaat gcggttcgct caaccagtgg ctcgaggttg caccgtccgc
     5521 acaggcagca tgcagtgtag taggagcgct cgtaagcgtg aatgactttg caattcggcc
     5581 agcgaaagga atggcagacg atgagatgct gaccactggc aaataccgtt ttcggttttg
     5641 ctcgacggcg catctaccac atggttggga tgcaggggta ttgtttgagg aggcaaatag
     5701 aacgcttctc tgctcagacc ttttccatca tgatggtgat gtggaaccgc taaccgagtc
     5761 ggacataata gaacgggtgc ggaagacact gatcgaatac caggctagcc cttttgcaaa
     5821 ttacatacct tatacgccgc aaacagatca gatccttcag agattggctg cccttaagcc
     5881 caagacacta gcgacaatgc acggctcgtc ctttgttggg gacggcgagc gtgctctact
     5941 agaccttgct ttggtaataa aggaaacact tggttcgcca tgatttgtga agtagaacat
     6001 gataatcacg actccttcaa cgcaatgata aacaaattaa ttctcgatta aatcaagaat
     6061 tccaggaaag aaaaggcatc actacgaaaa gggagaaaat aacgtgaata gagaaaagaa
     6121 tgtaagtggt tttactcttc agccgggcat aggtaagtcg ctttgggttc taggtgatct
     6181 ttatactttt aaggttgtgg gtgaagaaac aggtggggcc attgcagtac tcgaggttca
     6241 agtgcagcca caaaacggac cacctcccca tatccaccat cgtgaagacg aaacgtttta
     6301 tgtgttagag ggagagtttt ctttcctgca tggtgacact acatttacag cgaccgctgg
     6361 atcgtttgtg tacattccca agggtacggt tcatacattc aaaaacgtgg gaacgtcccg
     6421 aggtaggttt ctggttatta tcacgcctgc gggattcgag aagttctttg aaaaaatcgg
     6481 tgagccagtg acagacaaga cttctccgcc tcctttcaat ccggaaactg tcaagaagct
     6541 tttggcgctc gctccgaact tccatctcga ggtgaagttg ccaccagatt cttagactta
     6601 cgaacgctat tcaataaata tcgatcagca taattggtga ctatatgttt ttaacaatag
     6661 atcaaggttc ttgtaaaaga aaaaaatctt gataacattt agaatgacaa aaaagatatg
     6721 aagataatcg ttatatttat aatagcactt tgttaccatt tcgcatgttc gaatgagagg
     6781 gaccttaaca aaatcgaagg cgaaataaag caagataaat taagatcaca agatttcact
     6841 aaagatccca ccaaattctt tgcacatcgc ggattgctaa attttgcccc tgaaaataca
     6901 ttgctagctt ttgaaatagc taaagaacgc ggtttcaaaa atatagagtt agatattgtg
     6961 acaacgaaag acaacaaaat agtagtatct catgatttca atttaaaaag aatgtttaaa
     7021 atcaatcggg atgtatgcaa agttaattat gatgaactac tagaacttaa gccatctcaa
     7081 tacttttttg aagcagttct aggtcttccc gaagaaaaaa taatcgctat tcgatctacg
     7141 gatggtccct ggataaaaag acttgattct caacaaatct cattgctcga tgatgtcttt
     7201 aaaacttttg gcaaggattt taattatcat ttagatatca aaataattgg ttgtgaacaa
     7261 gagcttattc aagaactcgt ttctgttatc gaaaaatatg atcttgaaaa taatgtcttc
     7321 attgaatcaa caagattaaa acttttggaa aagataattt caaccaatcc aaaaataaaa
     7381 acgatttatt ggaatgatga aatattttct ttctcagacg aggaatggca agatttagta
     7441 gagaaaggaa ttgattctgt tgacttacgg tattcaaata ttaataaaaa agaagtcttg
     7501 agaataaaat cacaaataaa aaatataagg attcacacat atactgttaa ctcactcaag
     7561 gtgattaaca gtttggataa ctttgtagat tatataatca ctgatttaga tatatttgga
     7621 aataatgttg acaagtatcg atttttcaat cagaaatcac aaaaaattaa actttcttct
     7681 cctttatacc agaaattcaa atcaatctta tcctacttca aataatttgt ttcagcataa
     7741 gcttacgttg ctcagatgtt ctcagttaga ataattaaat attgaatatg aataaaattt
     7801 gttccataac gaagcaaatg tagtttattt cgtaatttaa ttattcttat tggctctaag
     7861 ggctggaatc agatcaccgt gaataataag atctgagatt tcgagccttt ctaaagcctt
     7921 tagacgtttc tcttcaagtg gtttacagtc agtgattcga cctgttgagg cttcatagca
     7981 ggcagattgg gaggtttgcc taaaacggtt aataaaaata tacttaccgt cggcgaaact
     8041 cccattccga aatacaacca tggagttttc accatttgta agatcacttc caagcatcac
     8101 gtttttttca tcaattaccc caagaagagc taagacggtt ggagcaatat caagatgacc
     8161 acctgtgact ttcctcatcc ccgcttcttc accgtaagga agacgaataa tcaaaggtaa
     8221 tcgttgtagg agcatccatg tgcggtactc actagattct gggtatccaa gaagcttttt
     8281 aatatattct aattcaatat gtttagattt atggtcaccg taaacaacaa cgatagacct
     8341 atctaaaaga cctgactttc tgagtccatt cacaaatgtg ccgaacactt tgtcaaagta
     8401 gtgaacggag tgtaagtagt tgcctatcat gcttccttcc atttcgccaa gatttagttc
     8461 atgatacttt ttgggtacca tgtagggatg gtgagtcgag tgagtgatta ggtatgctaa
     8521 gaaaggttct cgtcgggcct gaagtatagg aattgtttgg atgaagaact cataatctga
     8581 taaccaacca tttatcgtct cagtaatgtt gtatttatct tggaagtatg actgttgaaa
     8641 accaagtttt ttatgcatcc tttccatatt ccagaacgaa gcggcttcag cagttgcaga
     8701 aagtgttgta aaaccatgtt gcgataagat ggcggggagt ccgaagaagg acctagaatg
     8761 gtaattcata gatactgttc cacgagctac tggataaagc gattgtaatg agataaactc
     8821 accgtctgaa gttgatccca agtgagcttg gtcatagaaa ttgatgaagt aaaggctttc
     8881 cttagaaaat gcagtcaaat gtggagtgat gggttgccca ttaacttcga gtccgattgg
     8941 aaatgaattc agtgactcca ccatgatcat gataacattt ctacctttag cgataccaaa
     9001 taaattagat ctgctatttc tttcgttatg ctggctgcct aagaaatttt tcacacgttc
     9061 tagattaact tctcctcttt tctcgtgcca tcctcctatg tgtgacaaag catcaacaat
     9121 gtggtatgac aacaagccta ttgttccaca ggcatctcgt tgaattgtat tgggagtaaa
     9181 taaagcatat ttatcattcc aaactagtct taaagtcgga gcagcgatga gaatgcttgc
     9241 tactaatgat cctgctgaga ttcgtttaat atatttccaa tcaagaggtt gaatttgttt
     9301 acaggcttta ctataaaaag gcaataatag aattccccaa atgatatcaa ggtagtatcc
     9361 cagatctgag ggctttagca gttttatgat gctttccgat accttcggaa gcataaatac
     9421 acttgaaatt tctggaattg atataatctc tgaaaaccat aaaacatgca gtttgtcagc
     9481 tattattaac gaagttaata ggaaatctaa taaaagcaaa accacaagac gacttattcg
     9541 aggtaaaaaa ataactagtg aaaatagggg gagaatactg gcaaggttag cagtgtatag
     9601 gttaggatgc gcttctatcc atttgttaaa tggctcgttc caaccccacc atacatattc
     9661 taatgaataa tagaaataga ctattttgca ccaaaatgag gctataacca caatcggagg
     9721 gccgatttcc agtaaccaga cgctataaga gctgttcttt atttggtata gttttgaaag
     9781 atccattctt gtagcttact tgcttaactt tcatgcatgg aaattaaatt aggcgattct
     9841 gtattatgct agtttcatca aaaaacttgc tttactaaat taaatatcgg cttactatac
     9901 cacagggatt aataggtctc aaagaatttg ttattttgcc tatagattga ctatagattg
     9961 acatagccta tagattaaat cgcatttgaa gagaagaaga aatagtattt aattgctgat
    10021 gaataaatca ctagtatatg gtaaagattt acataaacta ttaatggctt gccagagcgt
    10081 tttttatgac tatattattt ttacaaacac tgtaggagga agtattcatg aagcggttat
    10141 caacactact ggttttaatt ctctctgttt tcgtaataac tatgatagga tgcgagaagc
    10201 tggaggaggg aacaaaaccg cagaaaaaaa ccgataccga aaaaacaacg gagaaaaaag
    10261 aggatatttt agaaacacca agagtccaag agctgccttc ttttgcagat ttagtcgaga
    10321 aactaaagcc ctcagtttta aacataagta caacaagcac atttagtcag agaggccttt
    10381 ttcctaaatc accttttggt gaagaaaatc catttgaaga attcttcaag agatttttcg
    10441 gcgacattcc acaacaagaa tttaagcaaa gcggacttgg ttccggattt atcattagtg
    10501 aagatggata cgttgtgaca aataatcatg ttattgataa ggctcaggac atagaggttg
    10561 tgctagaaga tggaaagaaa tacaaggcag atattatagg gaaagatgct aaaacagacc
    10621 ttgctgtact taagattaat cctgagaaaa tgttacaggc tgtagtgttt gggaactccg
    10681 ataaacttcg gattggagat tgggtagtag caataggaaa cccttttggg cttggttata
    10741 cagtaacagt aggtatcgtg agtgcaaagg gaaggtctct tggtcttagt agttatgatg
    10801 attttataca gacggatgcc tcacttaacc ctggaaacag tggaggccct ctttttaatc
    10861 aaatgggaga tgtaatcggg gtgaataccg ccatagttgc aggaggtcag ggtataggat
    10921 ttgcaatacc aattaacatg gcaaagaacg taattgaaca gcttaaggat aaaggcaaag
    10981 ttgttcgtgg atgcctagga gtacttgtac agccaattac acctgctatt gctgaaagca
    11041 tgaaacttaa agaacccaag ggtgcattag taggcgatgt aacccctaat agtcctgcag
    11101 agaaggccgg aattcaaagg ggagacgtta ttgttgattt taatggaaat aagatcgaag
    11161 atgtatcaga ccttaccaca cttgctgcgg ttactccacc cggtacagag gtaaaattga
    11221 aggtgatgaa ggatggatct gtaaaagaca tagatgttaa gcttggagaa tttccggatg
    11281 aggtagcaga atcaacagag aaagaggtaa aggaaaaact aggtcttaca gttcatgaga
    11341 taaccccgtc gattgcagca cgatttagac ttgaaacgga taagggtgtt gttataacac
    11401 acgttgacca gggaagcgtt gctcaagaat ccggtttgca accaggagat gtgattttgg
    11461 atgtagatgg tcaagctgtt aacaatatag atgattataa gaatgctata gacaaaatgg
    11521 agaagggcaa gtcggctctc tttcttatta aaagaggtaa aaatacgata tacgtaggaa
    11581 ttagaactga atgatttcct agctactttc tctcactgct ttggaaaagt agcctacaaa
    11641 ttcctcaggt ttcatattcg ctatacggga caactttcta atctgctctg ggttttctaa
    11701 atcctctttt gtgagcttta tcatatattt ttctgcaacc tcataggctt ctgtattt
//
LOCUS       DATDRL010000170         2928 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3296562, whole genome shotgun sequence.
ACCESSION   DATDRL010000170 DATDRL010000000
VERSION     DATDRL010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3296562"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            413..1483
                     /gene="aroC"
                     /locus_tag="VI935_05990"
     CDS             413..1483
                     /gene="aroC"
                     /locus_tag="VI935_05990"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HLE25185.1"
                     /translation="MPGNSFGRLFQITTWGESHGPALGVIIDGCPAGLVISEEEIQYE
                     LDRRRPGQSKVTTQRKEPDKVEILSGVFEGKTLGTPISIMIKNVDVISKSYEDIKDIY
                     RPGHADYTYDVKYGVRDWRGGGRSSARETVGRVAAAAFAKKILNLNGVKTIGYVKQIG
                     DIVAEEIDFNEIENNPVRCPDPVKAIEMINLIEKVRNEGDSIGGTVEIVSIGVPTGLG
                     DPVFNKIDADLAQALMSIGGIRGFEVGMGFEVSRRKGSEVNDVMYIDDSNRLRFKTNN
                     AGGILGGITSGENLVVRIAIKPTSSIPKTQETVDKHGNIKELVVKGRHDPCLCPRAVP
                     IAEAMVNLVLVDHLLMSKVARI"
     gene            1725..2255
                     /locus_tag="VI935_05995"
     CDS             1725..2255
                     /locus_tag="VI935_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HLE25186.1"
                     /translation="MELKGKRVAILAENQYQELELWYPLYRFREEGAEVVVVGSGSSD
                     MYHSKHGYPVKVDITADKVKGVDFDCVIIPGGYAPDYMRRYPAMLKLVKDAFDHGKVI
                     GAICHAGWVLVSAGILRGKKVTCVSAIRDDIVNAGAEYINAEVVRDGNLVTSRIPDDL
                     PAFMREIIRAIWEKKG"
     gene            2635..>2928
                     /locus_tag="VI935_06000"
     CDS             2635..>2928
                     /locus_tag="VI935_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HLE25187.1"
                     /translation="MSEMVTQKRKNRLVDESSAYLRSASHQPVDWYPWCEEAFRKAKE
                     LDRPVLLDIGAVWCHWCHVIDRESYEDEEIAKIINANFIAIKVDRDERPDVDRR"
BASE COUNT          882 a          517 c          726 g          803 t
ORIGIN      
        1 ttactgaaat tggctataac cttataaaaa ttcgcccatt tgatatgttt ccacagactt
       61 atcatattga atctctagca ttgctttcaa gactcatatg aagcgggact agaaagtccc
      121 gcctatcccc agagccttaa cgtacaccga taggcagggt tttctaaccc tgcacaatga
      181 tacgaggttt tccaatcccg tcaaccatgc ttcagtggat aaatatccgc tcatcctaag
      241 ccagtcgaag ggttggaagc ctaaaagttt cctctgcatt taagagatgt aacataccat
      301 agcatacgaa taggtggggt tttctaaccc gttgctggac aggtatatcc cgaccaagtt
      361 tctaatcccg taagcatttg caatgccttt cctgctgata tagaatttcc ctatgcctgg
      421 aaattctttt ggtaggcttt ttcagataac tacctggggt gaatctcacg gtcctgctct
      481 tggggtaata atagatggct gtcctgcagg gcttgtgatc tcggaagagg agattcagta
      541 tgaactcgat cgtcgccgcc caggtcagag taaggtaacc acccagagaa aagaacctga
      601 caaagtggag atactttctg gcgtgtttga aggaaaaacc cttggcacac ccatctcgat
      661 tatgattaaa aatgtggatg tgatttcaaa atcctacgaa gacataaaag acatttatcg
      721 tcccggccat gccgattata cctatgacgt taaatatggt gtgagggact ggagaggagg
      781 cggaagatct tcagcaagag aaaccgttgg acgcgttgcc gctgcggctt ttgcaaagaa
      841 aatcctgaat ttaaatgggg taaagactat tggatatgta aagcaaattg gagatattgt
      901 ggctgaggaa atagatttta atgagattga aaataatcca gttagatgtc cggatccagt
      961 aaaggcgata gagatgataa accttattga aaaggtaagg aatgaaggcg attcaattgg
     1021 tggaacagtt gagattgtat caatcggggt ccctacaggt cttggcgacc cggtgtttaa
     1081 taaaattgac gccgaccttg cgcaagccct tatgagtata ggtggtatca gaggattcga
     1141 ggtaggaatg gggtttgagg tttcaagaag aaaagggtct gaggtcaacg atgttatgta
     1201 tattgatgat tctaatagac tcagatttaa aacaaacaat gcaggtggaa tccttggtgg
     1261 gataacaagt ggagagaatt tagtagttag gattgcgata aaaccaacct cttcgattcc
     1321 aaagactcaa gaaaccgtgg ataagcatgg caacattaag gagcttgttg taaaaggtag
     1381 gcacgatcct tgcctttgtc cgagggcagt tcccatcgct gaggcaatgg taaatttggt
     1441 attagttgat catttattaa tgtcgaaggt tgcaagaatt taagactata gtctaggttt
     1501 gaactatatg caattgtaat tgcaagtgac tcaaaggaag aaagcgaccc cttagtttta
     1561 tttgtcattg cgaacgaagt gaagcaatct gtttgagatt catcactacg gaacgacaca
     1621 gtctgtcgta ttgattagtc gctttcctct tcacattgga attaagtcct ttatttgtcg
     1681 cacaacacta gttatttata attcttgaat gtagggaggt aattatggag ctaaagggaa
     1741 aaagagtcgc gattttagct gagaaccaat atcaagaatt agaactttgg tatccgcttt
     1801 ataggtttag agaagaagga gcggaggtag ttgtagtagg aagtggaagt tctgatatgt
     1861 atcatagtaa acatggttat cctgtaaaag tggacattac tgcagacaag gttaaaggtg
     1921 tggatttcga ttgtgtcatt atccccggag gttatgcccc agattatatg cgccgttacc
     1981 cagcaatgct aaaactcgtt aaagatgcct ttgatcatgg aaaggtaatt ggagctatct
     2041 gccatgctgg atgggtgttg gtctcggctg gaatactaag gggcaaaaag gtgacctgcg
     2101 ttagtgctat tagggatgac attgtaaatg caggtgctga gtatataaat gctgaggttg
     2161 taagggatgg aaacttggtg acatcgcgta tccccgatga cctgccagct tttatgcgcg
     2221 agattattag agcgatatgg gagaaaaagg ggtaacctaa gtgtcgtggg acggcatccc
     2281 ttccccgata aaatcgggga cttggctcag ggcaggctat gccgcaatca attccaatta
     2341 taaaaatccg cgactggaag gtcgcggcta acgaaggctc catacaaata attacataac
     2401 gatagccgag gttttccaac atcggataat aatgaacttg catcctttag agatacttat
     2461 gcctaacagg aaaccgttat ccccgtttgt tctgagcatg cctaagacta agtaggatac
     2521 atcgggaaag gcttgcttgt ggacggccat gtagggacaa ggcatgactt gcccataaaa
     2581 ttgcatgtcc tatttgaaga tgagccacta attgtttaat tttaatatgt tattatgagt
     2641 gaaatggtga cacaaaaaag aaagaatcgg ttagtagatg aatcaagtgc atatttaaga
     2701 agcgcgagtc atcagcctgt agactggtat ccttggtgtg aagaggcatt ccggaaggca
     2761 aaggagttag accgtcctgt tctccttgat atcggtgctg tatggtgcca ctggtgccat
     2821 gttattgaca gggaaagtta tgaggatgaa gagattgcaa aaataatcaa cgcgaatttt
     2881 atcgcaataa aagtggatcg ggatgaaaga ccagatgttg atagaaga
//
LOCUS       DATDRL010000171         9395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_636452, whole genome shotgun sequence.
ACCESSION   DATDRL010000171 DATDRL010000000
VERSION     DATDRL010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9395
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636452"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VI935_06005"
     CDS             complement(<1..332)
                     /locus_tag="VI935_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HLE25188.1"
                     /translation="MQKNGFRIETDSMGEMMVPTSAYYGAQTARAIENFPISGIRKPR
                     TFIKALGMIKLAAAQANMELGLLNKRLGSAITKAAKEVIDGKLDDHFVLDVFQTGSGT
                     STNMNANEV"
     gene            complement(851..1693)
                     /locus_tag="VI935_06010"
     CDS             complement(851..1693)
                     /locus_tag="VI935_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HLE25189.1"
                     /translation="MTEVTSTWFTKSCPHFYLLCFHCNYIQGAGSPDQVTVNAVCMGM
                     IAAGVATMLQWIWKGPVGSGYLAPPVFSAIFLGPSVLAAKTAGLPVVFAMTIFAGAIE
                     VFLSRFLHRLRGVFHPAVSGFIVLIVGIELGIVGIEQVLDIETLNDPKFDQHIFVALI
                     TLTIIMGLNVWFRGLMRVLCSMIGILIGFIISIPLGLVSLKSASAFLHSPFFTFPDLG
                     YITYHFDPSLIPSFLTAGIAAVLRTIGVITTCQKINIEVGSVCPIILTKTPIEAVESA
                     DIVP"
     gene            complement(2219..5410)
                     /locus_tag="VI935_06015"
     CDS             complement(2219..5410)
                     /locus_tag="VI935_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLE25190.1"
                     /translation="MKIVDESIKHPVSVIVGVIFILMFGIISLIKLPVQLTPDVEKPR
                     ITVDTTWIGGSPQEVESEIVREQEDELKSVEGLVEMTSESADGSGSIVLEFGLGTNLD
                     AALLKVSNRLDQVKEYPFEADKPVIRSVDVRGSAMAWFILKPLPGKDIYIDKLHDFAE
                     DFIKPRFERVPGVASSNVFGGQEREMQVVVDPDALAARGITLFEMAQALDKENENISG
                     GDFEEGKRRYIVRTVGEYGSPEDIENVVITRKNGVRVYVRDVATVRLGYKDHEYAVRQ
                     NGQPAIAINALRETGANAIDVMAGLREAMMELNEGILKDRGLVLYNVYDETNYINNSI
                     DLVEKDVVIGSILAMAVLLLFLRSGSSTMIISIAIPISIIGSFIMMMFFRRTINVISL
                     AGMGFAIGIFIDNSIVVLENIYRHIQMGKSRLKAAYDGTVEVWGAILASTLTNVAVFL
                     PVIFVQEQSGQLFRDIAIAISFAVLLNLIVSITLIPSAAAQFLKVAQVKKVSSFENLW
                     GYIRVAQGFTDGVTNLVRQIIENKRLRLTVVLAFTLGSILLSWAMVPKMEYLPEGNRN
                     LIFGILLPPPGYNINEFTQIGEQIESDLRPYWEADAGSPEAEKLGGPPILNFFYVARG
                     RQVFMGAVAKEPSRVRELIPVMQGTLSKIPGMIAVVTQSSLFQRGIGEGRAINIQITG
                     PDLEKLVSLGQNIFFGVLQTIPEAQARPIPSLDLGNPEVQVITDRVRASDVGVTNEEL
                     GFSVNALVDGAKVSDYLIEGDEIDLALRGDDRYSNRTQDIENLMINTPSGKMVTVGSI
                     ARVNVTNGPEQINHYERQRTITIQVIPPEKVPLESAIEKIQENIINSLRLSGEIGGSY
                     NIILTGTADDLSVARKAFQWNFILAAVITYLLMASLYESFLYSFVIMFSVPLASFGGF
                     LGLFLLNRFTYQPLDVLTMLGFIILVGTVVNNAILIVHQSLNYMRDEGMELRDALTES
                     VRTRIRPIFMSTTTGIFGMLPLVVFPGSGSELYRGLGSVVLGGLLVSTVFTLFLVPAV
                     FSTVVDIKEGLKYKSSKIWHAFAQIPSKILSIF"
     gene            complement(5541..6635)
                     /locus_tag="VI935_06020"
     CDS             complement(5541..6635)
                     /locus_tag="VI935_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLE25191.1"
                     /translation="MNITRIVFPLLLISLILGFVVIFPVKAQAPPPSPVVVAKVEEQK
                     VQRPITLVGNAEPSKRSLIASEISGLVDRFPVKEGDFVKKGDVLAIFETEPLEIRLRE
                     AKAAKREAQARYELAQKNLKRFQELKNKGIASVQQFQDAESEQKAWSAEISQLQAQID
                     RYEYDLDKAKIVAPFSGYITIERTEVGQWIDEGGPVVELIDIDTVEITVDTPERYVSQ
                     IKLNDKATVKFDALPNVTLEGKVISIVPQADKAARTFPVKIEIDNKDHLVKSGMVALV
                     SFLIGEPSLVKLVPKDAIVERNNLKFVIVVNNGTAQPVTVNTGVAYKNLIEIIGPINI
                     GQPVVIRGNERLQPGQPVKVVNQDGMPEKK"
     gene            7359..9077
                     /gene="asnB"
                     /locus_tag="VI935_06025"
     CDS             7359..9077
                     /gene="asnB"
                     /locus_tag="VI935_06025"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HLE25192.1"
                     /translation="MGKFFGIIDGENLVIQDIFSDEGDKLWVSYEGEIYNSDEIRLSS
                     ESFGYRFNPGAYSELIAKAYKTWGEDFAKRLNGAFSICIIDKEKEILYLIRDQVGRKP
                     VYFSDANGRFAFSSDVKSLLEIPSFPREIDLRALNFYLTYRYIPQELSIFNHIRRLPA
                     GCALRLNLRSREIKKWSYWEPPTVEERLANEDELLKELEKIIEDAIRIRMRGNSHTGA
                     FLSGGLDSSLIVAMMSRLSSNTLKTFSVGYNDEKYNEIPFSRIVSRYFATDHEEFIIE
                     PDFDAFLESCSFFDEPLGDPSIIPTYYAMKLGKKYVDSVISGDGADGLFLGFRTHYLS
                     SRYEEIKRYMIPPFEWLSGAVAGILPQEVKWRIFFENKTPEDLFLTRGIVFNAHLRKR
                     LFKNWVLDELFREHFSEPEIYGTSTMNSYSGTLSGKMAFLTFKSDPDDILFKIDKISR
                     NLSLNVRTPFLDTRLVEFALGKVPGNMKIRGDKTKYILKRLGAKFLPKELPLDRKRGF
                     NPPLFKSLRKEWSHKARDILMEGEDNFLIRVILKNLLIYIKILYSMKVGGYLSFLTLK
                     SGGMGT"
BASE COUNT         2809 a         1918 c         1853 g         2815 t
ORIGIN      
        1 acctcgttgg cattcatgtt ggttgaagtg cctgagccag tttggaatac gtcaagcaca
       61 aagtggtcat ccagttttcc atcaatcact tcctttgccg cttttgttat agcgctccct
      121 agtcttttat ttagaagacc aagctccatg ttagcttggg ctgcggcaag tttaatcatt
      181 cctaaagcct taataaacgt cctcggttta cgaattccgc ttattggaaa gttctctata
      241 gctcttgctg tctgggcgcc gtaataagca gatgtaggaa ccatcatttc gcccattgaa
      301 tcggtttcaa ttctaaatcc atttttttgc ataattatat tcctccttat aattttctat
      361 tagactttct tgggtttacc aattaaatat atctgaattg attgtaaata taatttatct
      421 tcatggatag ttatgcggtt aatataatta taccatttga atttagaaat ggttcataga
      481 aaatagtgcg tgaccaaatt aattttggtt tccccagggg ttacgcaaga tcgccgttca
      541 tggttctaca ggctcaccac gaacgtcttg attttagtac cgttcacccg ttcggttgag
      601 cctgcgtcaa ggtcagtcgg gcgctcacga tgaagcctga gcttgtctaa gggcgtactc
      661 atttgtgtaa gcgtatcaaa tttaaatttg aggaccacta attcaatagc tttagcattc
      721 aagcgttatg tgtttaggct tcggcttaat gcgtcatcta agctatggat tttgtggaac
      781 tatgtagtat cgttgttgat caagtagggc ttaatatggc agcctctcag aatatcaaac
      841 tcggcgttat ttatgggact atatcggcgc tttctactgc ctcgatgggt gtcttcgtca
      901 aaatgatagg acaaacactg cccacctcta tgttgatctt ttggcacgtt gttataaccc
      961 cgattgtgcg tagcactgca gcaatgccag ccgttagaaa ggacggtatg agcgaagggt
     1021 cgaaatgata agtgatataa ccaagatcag gaaatgtaaa aaatggtgaa tgtaagaatg
     1081 cactggccga ttttagggaa acgaggccta acgggatgga tatgataaaa ccgatgagga
     1141 ttccaatcat cgaacaaaga accctcataa gaccccgaaa ccagacgttt aaacccataa
     1201 taattgtaag cgtaatcaat gcaacgaaga tgtgttgatc aaactttggg tcgttaagcg
     1261 tttcgatgtc tagcacctgc tctattccaa caatgccgag ctcgataccg acaattaaaa
     1321 caatgaaccc agaaacagcg ggatgaaaaa cgccacgtaa gcgatgtaaa aaccttgaga
     1381 gaaaaacctc aatcgcacct gcaaagatcg tcatagcaaa tacgacaggt aagccggctg
     1441 tcttcgcagc aaggacggat gggccgagaa aaatcgcaga gaaaacagga ggagccagat
     1501 acccagatcc tactggaccc ttccagatcc actggagcat cgttgctacc ccggcagcaa
     1561 tcattcccat gcaaacggca ttgactgtga cctgatcagg actccctgcg ccttgaatat
     1621 aattacaatg aaaacaaagt agatagaaat gaggacaaga ttttgtaaac caagtagaag
     1681 taacctctgt aatggcggga tttcatctac cgtgtaaatc agatcatctg gacgtgttgc
     1741 cattgagatg tcttttctct attccaatcc atattaacac aagttgtaac ctatgaataa
     1801 actatgccaa atcgcaagat tacttgaagg taacgtagga ttgccatccc ttcgactccg
     1861 ctcaggacag gcccttcgac agagcctgtg gtgagtttaa tcgaaccact caggccaagc
     1921 cttgctgcga tgagttcctc ttataaaaac acgcgactga aaagtcgcgg ccatcgaagg
     1981 attgtattta aataatcaaa caacgattgc cgaggttttc caacctcgga taaaacaata
     2041 tgatatggat gcccaccgat taaatcgggt agctcgctca ggacattcct ggtgcgattc
     2101 atgttgcggc tagaagaggt tccctaacta cccttcctcc cttcgtcttt gctcaggaca
     2161 ggcttgatta ctaccccagt ccccgccgct cctacggatt tatgtaatat ggttaggact
     2221 aaaaaattga cagtatcttt gatggtattt gagcaaaagc gtgccagatt tttgaggatt
     2281 tatattttaa accctctttt atatcaacga ccgtgctaaa aacagcggga acaagaaaaa
     2341 gggtgaacac agtcgaaact aagagccccc ctaatactac gctgcctaag cccctataga
     2401 gctcggagcc agagccgggg aaaacaacta aaggaagcat accaaagata cccgttgttg
     2461 tgctcataaa gattggtctg atacgggttc ttacagattc agttaaggca tctctaagct
     2521 ccattccttc atcgcgcatg tagttaagcg actggtgaac aattaagatg gcgttattta
     2581 ctactgttcc aacaagtatt ataaacccaa gcattgtaag gacatcaagt ggctgatatg
     2641 tgaatctatt taatagaaaa agcccaagaa acccaccaaa agaggcaagg gggacactga
     2701 acattatcac aaaggaatat aggaagcttt cataaaggga tgccataagt agatatgtta
     2761 ttactgcagc gagaataaaa ttccactgaa atgcctttct tgcgacggat aaatcatctg
     2821 ccgttcctgt aaggataatg ttatatgatc cgccaatctc accgctaagt cgtaatgaat
     2881 ttataatatt ctcttgaatc ttctcgattg cactttcaag aggcaccttt tcaggtggta
     2941 taacctgtat tgtaattgtc ctttgacgct cgtagtggtt aatttgctca gggccatttg
     3001 taacattaac cctagcgatt gaacctacag tcaccatctt tccacttggc gtatttatca
     3061 tgaggttttc aatatcctgt gtgcggtttg aatatctgtc gtctcccctg agtgcaaggt
     3121 caatttcatc accttcgatg aggtagtcgc ttaccttagc tccgtcaaca agggcgttta
     3181 cgctaaatcc aagctcctca tttgttacgc caacatccga agctcttaca cggtctgtta
     3241 taacctgaac ctccgggttt cctaaatcaa gacttggaat cggtcttgct tgggcctctg
     3301 gaattgtttg cagtacaccg aaaaatatat tttgacctag gctaacgagt ttttccaagt
     3361 ctggtcctgt gatttgaata ttaattgccc gtccttcacc tatacccctt tggaaaaggc
     3421 ttgattgagt aacaaccgca atcatcccag ggatcttgct aagcgtacct tgcattacgg
     3481 gaataagttc tctcacccta cttggctctt tcgctacagc gcccatgaat acctgacgac
     3541 ctcttgccac gtagaaaaag tttagtattg gcggtccacc tagtttttcc gcttctggag
     3601 atccggcatc tgcttcccag tacggtctta aatcgctctc tatttgctct ccgatttgag
     3661 taaattcatt gatgttgtat ccgggtgggg ggagaagaat tccgaaaata agattacgat
     3721 ttccctcagg gagatattcc atcttgggta ccatagccca gctaagtaga attgatccaa
     3781 gtgtgaaagc aagaaccaca gtgagacgaa gtcttttatt ttctataatc tgtcgaacta
     3841 gattagtgac tccgtctgtg aatccctgag caacacggat ataaccccag aggttttcaa
     3901 aggaagaaac cttttttact tgtgctacct ttaagaattg agcggcggcg cttggaatta
     3961 aggtgataga tacaattaga ttaagcagaa cagcaaagct tattgcgatt gcaatatcac
     4021 ggaagagctg tcctgactgt tcttgaacaa agataacggg taaaaacaca gcgacgttag
     4081 taagagtact cgctagaata gcaccccata cctcaactgt gccgtcatac gccgccttga
     4141 gacgggattt gcccatttgt atatggcggt aaatgttctc taaaacaaca atggagttgt
     4201 ctataaaaat tccaatggca aagcccattc ccgcgaggct aatgacgttt atcgttcttc
     4261 taaaaaacat catcatgata aatgagccaa ttatgctaat aggaattgct attgaaataa
     4321 tcatggtact gcttccgctt cttaaaaaga gaagaagcac tgccatagcg aggatacttc
     4381 caattacaac gtccttctct acaaggtcta ttgagttgtt aatatagttc gtttcatcgt
     4441 agacgttata taaaaccaaa ccacggtctt ttaagatgcc ttcgtttagc tccatcatcg
     4501 cttcgcgaag gccagccatt acgtcaattg cgttagcgcc cgtttcccta agtgcattaa
     4561 ttgcaattgc cggttgacca ttttgcctaa cggcatactc atggtccttg tacccaagcc
     4621 ttacggttgc aacatcgcgt acgtaaacgc gcacaccatt tttcctggtg ataactacat
     4681 tctctatgtc ttcaggcgac ccgtattctc caacagttcg aactatatat ctcctctttc
     4741 cctcttcaaa atcaccaccg ctaatgtttt cgttttcttt gtcaagggct tgtgccattt
     4801 cgaataatgt tattccacgt gcagcaaggg catctggatc aactacgact tgcatctcac
     4861 gttcttgtcc accaaatacg tttgaagaag ctacaccagg tacgcgttca aatcggggct
     4921 ttatgaaatc ctctgcaaag tcatgaagct tatcaatgta aatatccttt ccaggaagcg
     4981 gctttaatat aaaccaggcc attgcacttc ctctgacgtc aacgctcctt ataactggtt
     5041 tgtctgcttc gaatggatac tcttttacct ggtcaagtct atttgatacc ttaaggaggg
     5101 ctgcatccag gttagttcca agcccgaact caaggacgat gcttccagaa ccatccgcgc
     5161 tttcactcgt catctcaact aagccttcta cgctctttaa ttcgtcctcc tgctcacgga
     5221 caatttcgct ttctacctcc tgtgggcttc caccaatcca tgtggtgtca accgtaatcc
     5281 tgggtttttc cacatcaggt gtaagttgaa caggtaattt aattagggaa ataattccaa
     5341 acataagtat aaaaatcaca ccaactatga cgcttacagg atgttttatt gattcatcaa
     5401 cgattttcat gagctttgaa cctcaaacgt aagtatctag ccactaaggc actaagacac
     5461 gaagaattta ggagtcagaa ttcagaagtc agaagaaata ataaagactt ctgactcccg
     5521 acttctagct cctgacttcc ctattttttt tctggcattc catcctgatt tactactttt
     5581 actggctgac cgggttgaag tctctcattt cccctaataa caaccggttg tccaatgtta
     5641 atgggtccta ttatctcaat aagattcttg taagctacac ctgtattaac cgtaacaggt
     5701 tgggcagttc cattattcac gacaatcaca aatttaagat tgttccgttc aacgatagcg
     5761 tcctttggga caagctttac taaagatggt tcgccaatca aaaatgaaac aagagcaacc
     5821 atcccacttt ttacgagatg gtctttattg tcaatctcga tttttacagg aaatgttcgt
     5881 gccgctttat cggcttgagg aacgatagaa atgacctttc cttcaagtgt tacatttggt
     5941 agagcatcga acttgactgt tgccttgtca ttcaatttaa tttgactaac ataacgctca
     6001 ggagtgtcaa cagtaatttc cactgtatct atatctataa gctctacaac cgggccgcct
     6061 tcgtcaatcc actgacccac ctctgtacgc tcaatagtta tgtagccact gaaaggtgca
     6121 acgattttcg ctttatcaag gtcatattca tacctgtcta tctgagcctg aagctgggaa
     6181 atttctgctg accacgcctt ttgctcagat tctgcatcct gaaattgctg aactgaagca
     6241 atgcctttat tttttagttc ttgaaaacgt tttaagtttt tttgagctag ctcataccgc
     6301 gcctgagcct cacgctttgc tgcctttgcc tcgcgaagcc taatctcaag aggttctgtt
     6361 tcaaagatag caagcacatc ccccttttta acaaaatcac cttcttttac agggaatcta
     6421 tctacaagcc ctgaaatttc acttgctata agactccttt tacttggttc ggcgttacca
     6481 acaagcgtaa tgggtctttg aaccttttgt tcttctactt tagctactac caccggagaa
     6541 ggaggtggag cttgagcctt gactgggaaa ataactacaa accctagaat aagagaaatt
     6601 aatagaagtg gaaaaacaat tctcgtaata ttcattttga atttcttata caatagctac
     6661 ttataaaaag attaagtaat tacttactta cattatcatt acagatttta ttgtcaaatt
     6721 aaccaataaa ataatttacc tgtttatggc ttattattga aactaaaccg attgatttat
     6781 atcaatttga tttagtgttt actgaacgtt gcttactatt ttagataagg agaaagaagg
     6841 gttagcattt ataacattca gtaaccaaca aaaggagaat aggaagcgat gcgcttgatt
     6901 gtggaatcag tgctaataca tgtaactaga gcttagtaga gatggaagta caagctatca
     6961 aataacaaag ggtaaattaa tatacttttt tgggctgtcc taaacccgga ggatggaatc
     7021 aaagggattc aacctgtgac ccgacaggct gaatatgaga ggtttaggac agctccaact
     7081 tactccatct taattaaatt atcaatgaat tgtattttca ataatactta actaattaat
     7141 accccacttt tatataatat acattaaatg taaaataaat aaaaccaatt ctaaaaaatt
     7201 atttcctgtt tcaagtaggg acgggatttc aactcatatg gtgtctaagt gactatgatg
     7261 tttatacttt tcaaataaag ggaaactcat agcattctac attaatcttt caagaaaggt
     7321 tttgtaaaaa cctctccatt atttatactg ctattttgat gggtaaattc ttcggcatca
     7381 ttgatggaga aaatttagtt attcaagata tcttttcaga cgagggagat aagctatggg
     7441 tttcctatga aggtgagatt tataattcgg atgaaataag attatcctca gaatcatttg
     7501 ggtatagatt caatcctggt gcatattctg agctaatagc taaagcatac aaaacatggg
     7561 gagaagattt tgcaaaaaga cttaatgggg cgttctcaat ctgcatcatt gataaagaaa
     7621 aagaaatcct atatttgata agagatcaag ttgggagaaa accagtttat ttttccgatg
     7681 caaatggaag gtttgctttt tcatcggatg tcaaatctct actcgaaatc ccaagttttc
     7741 caagagaaat tgatctaagg gctttaaact tctatctgac ataccgctac attccacaag
     7801 aactttctat ctttaatcat ataagaaggc ttcctgcggg atgtgcactt cgcctcaatt
     7861 taaggtccag agagattaaa aaatggagtt attgggaacc cccaacggtt gaagaaaggt
     7921 tagccaatga agatgagctg ttaaaggaac tagaaaaaat aatagaggat gctataagga
     7981 taaggatgag aggtaattct catacaggtg catttcttag cggtgggcta gactcaagtc
     8041 tgatcgtggc gatgatgagc agattgtcat caaatacatt aaaaactttt agcgttggat
     8101 acaatgatga gaaatataat gaaatcccgt tttcaaggat tgtttcaaga tactttgcga
     8161 cagaccatga ggaatttatt attgagcccg actttgatgc cttcttggag tcttgttctt
     8221 tttttgatga accactcggt gacccttcaa ttatcccaac gtattatgca atgaaactag
     8281 ggaagaagta cgtagattct gtaatctccg gggatggagc agacggcctt tttttaggat
     8341 ttagaactca ctatttgtct tcgagatatg aagaaattaa aagatatatg attcctcctt
     8401 tcgaatggtt atctggcgca gtagcaggga ttttacctca agaggttaag tggcggatat
     8461 tttttgagaa taaaacccct gaggatttgt ttttgacaag gggtattgtt tttaatgccc
     8521 atttaagaaa aagactgttt aagaattggg tcttagatga gctttttaga gaacactttt
     8581 ctgaaccaga gatctacgga acatctacta tgaattcgta tagtggcacc cttagcggta
     8641 agatggcttt tttgaccttt aaatctgacc ccgacgatat tttattcaag attgataaga
     8701 ttagtagaaa cctctcatta aatgtaagga caccattttt agatactaga ctcgtggaat
     8761 ttgcacttgg gaaagtaccg ggcaatatga agattagagg agataagaca aaatacattt
     8821 taaaaagact tggggcgaag tttcttccca aagaacttcc gctagataga aagcggggtt
     8881 ttaatccccc attattcaag tcgcttagaa aggaatggtc gcataaggcg agggatattc
     8941 ttatggaagg agaggacaat tttttaataa gggttatatt gaaaaacctc ttaatataca
     9001 taaaaatcct atattcgatg aaggtcggag gctatctttc cttcttaact ttaaaatctg
     9061 ggggaatggg cacttaactg tagacagatt aagaaaacta ttttatttgt agccgaaaga
     9121 gcggttataa atttctactt taagagatag gtaattaaca tacgaaactt acagtggtgt
     9181 aaccataact ttatgaattc tatatacttt atgagatctt gaagaagggg tagaagaata
     9241 tgaagatagt tccccctatt attataattg gaatgagccg atctggtagc aatattctaa
     9301 cccgcatttt agaggattta ggtctatttg taggcaaaaa aaaagataga aataatgaag
     9361 ccattttttt taatcagtta aacgtatggc tttta
//
LOCUS       DATDRL010000172         2016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_539419, whole genome shotgun sequence.
ACCESSION   DATDRL010000172 DATDRL010000000
VERSION     DATDRL010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539419"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..452
                     /gene="rpsG"
                     /locus_tag="VI935_06030"
     CDS             3..452
                     /gene="rpsG"
                     /locus_tag="VI935_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HLE25193.1"
                     /translation="MQPDVKYKSKLVSKFINSLMRKGKKSVAERVFYGAMEVIEKKIT
                     DVVPLEMFETAVNNVKPLVEVRSKRVGGATYQVPVEVPKQRQLSLAFRWIIDAAKGKK
                     GRPMFQRLADELVDAYKKQGIAITQRENTHKMAEANKAFAHFAWSKF"
     gene            471..>2016
                     /gene="fusA"
                     /locus_tag="VI935_06035"
     CDS             471..>2016
                     /gene="fusA"
                     /locus_tag="VI935_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HLE25194.1"
                     /translation="MNIEKMRNFGIAAHIDAGKTTTSERILYYTGESYKMGEVHDGTA
                     VMDWMEEEQKRGITITAAATTCAWNDYQFNLIDTPGHVDFTIEVERSLRVLDGAICVF
                     CGVGGVEAQSETVWRQADRYGVPRICFVNKMDRIGADFMKVVSEINERLGIKAIPIQI
                     PLGKEQEFKGVIDLVNMKAIVYSDDIDKLGVNFSLEEIPAEYKKEAEIQREKMIEGLA
                     EQVDFLTEKFLNGEKITNDEIKKAIREGAINLKLVPVMCGSAFKNKGVQPLLDAVCDY
                     LPSPLDRKSIEGTNPYTDEEISRKPFPDEHFSALAFKIASDKHGDLTFIRVYSGKVTS
                     GTRVINSGKDKKELISRIYKMHANIREQVDELTAGEIGAVIGLKYTVTGDTLSDSDHP
                     IVLEKMEFPDTVISMAIEPKTDAEKEKLGVALSKLAKEDPTFKVRFDKETGQMIISGM
                     GELHLEVIKNRMLSEYKVDANVGAPKVSYRETIGKKVEVEGKFIQQTGGHGQFGHVWI
                     TLEPFKG"
BASE COUNT          689 a          301 c          498 g          528 t
ORIGIN      
        1 ttctgcaacc agacgttaaa tataagagta agctggtttc aaaatttatt aatagcctca
       61 tgcgaaaagg gaaaaaaagc gttgccgaaa gagtatttta tggggcaatg gaagttatag
      121 aaaaaaagat aacggatgta gtgccattag aaatgttcga aacagcagta aataatgtga
      181 aaccgttagt agaggttcga tcaaaacgag tcggtggcgc aacgtatcag gtacctgtag
      241 aggtgccaaa acaaaggcaa ttatcgttgg cgtttcgttg gataatagac gctgcaaagg
      301 ggaaaaaagg gcgtcccatg ttccagagat tggctgatga attggtagat gcatataaaa
      361 agcaaggaat agccataacc cagcgggaaa atacacacaa gatggctgag gctaataagg
      421 cgtttgctca ctttgcatgg tcaaaatttt agtacaggaa gtttaataat atgaatattg
      481 aaaaaatgag aaattttggt attgcagcgc atattgatgc tggtaagaca actacttcag
      541 aacgtattct ctattatacg ggggagtcgt ataagatggg tgaagtccat gatggtactg
      601 cggtaatgga ttggatggaa gaagaacaaa aacgtggaat caccatcacg gcagctgcaa
      661 caacttgtgc ttggaatgat taccaattta atttaattga tacgcctggt catgtggatt
      721 ttactataga ggtggaacga tcacttcgtg tgctagatgg ggccatttgc gtattctgcg
      781 gtgtgggtgg tgttgaggca caatctgaaa cggtgtggcg tcaggctgat agatatggcg
      841 taccgaggat atgtttcgtg aataagatgg atcgaatagg tgcagatttt atgaaggtcg
      901 ttagtgaaat taacgaacga ttgggaataa aagcgattcc tattcagata ccccttggta
      961 aagaacaaga atttaaaggt gttatagatc ttgtgaacat gaaggctata gtatactcag
     1021 acgacattga taaattggga gtgaattttt cattagaaga aattcctgct gaatataaaa
     1081 aagaagccga aatacaacgc gaaaagatga tcgaaggact tgctgaacaa gtggatttct
     1141 taacggagaa atttttgaat ggcgagaaaa taacaaacga cgagataaaa aaagccatcc
     1201 gtgagggcgc cattaatttg aaacttgtgc ctgtaatgtg tggttctgca tttaagaata
     1261 aaggagtgca gccgcttctt gatgctgtat gtgattactt gccctctcca ctcgatagaa
     1321 aatcaattga gggtacgaat ccttatacag atgaagaaat atcacggaaa ccgtttcccg
     1381 atgaacattt tagcgccctt gctttcaaaa ttgcatccga taaacatggc gacctgacat
     1441 ttataagggt ttattccggg aaagtaactt ctggcacaag ggtaataaat tccgggaaag
     1501 ataaaaaaga actaattagc cgtatttata aaatgcacgc taacattagg gaacaagtag
     1561 atgagctaac cgccggtgaa ataggtgcag taattggtct gaagtatacg gtaactggag
     1621 ataccttgag tgattcagat catcccatag ttcttgagaa gatggagttc ccagatacgg
     1681 ttatatcaat ggcaatagag ccaaagacag atgctgaaaa agaaaaatta ggagtggcct
     1741 tatcaaaatt agcaaaagaa gaccctacat tcaaagttcg ctttgacaaa gagacaggtc
     1801 aaatgattat ttctggtatg ggtgagttgc atttggaagt aattaaaaat agaatgttga
     1861 gtgaatacaa agtagatgcc aatgtagggg ctccaaaggt gtcttacaga gaaactattg
     1921 gtaaaaaggt tgaggttgaa ggtaaattta ttcaacagac aggtggtcac ggccaatttg
     1981 gacatgtgtg gattacattg gaaccattta agggtg
//
LOCUS       DATDRL010000173         3676 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2926270, whole genome shotgun sequence.
ACCESSION   DATDRL010000173 DATDRL010000000
VERSION     DATDRL010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2926270"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..454
                     /locus_tag="VI935_06040"
     CDS             143..454
                     /locus_tag="VI935_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25195.1"
                     /translation="MTWGDIISFFFILVLNPYGVVGIAIALLMVATAFIFFGVEAITV
                     PYRYVVKRFYCPLRKTNVELKLRPSIFTYRAYDDVIKCSAFKGKVTCKKGCLDLPELR
                     T"
     gene            897..1481
                     /locus_tag="VI935_06045"
     CDS             897..1481
                     /locus_tag="VI935_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25196.1"
                     /translation="MRKMILVLVVISSLATVGILAIMAADPGRYRPPTPSWFTGSTED
                     KMQKLGLIQPSYSDLMLWTGIRLNELFAAGTSGKTEYAKLQAKKIEETLEKAALVDPT
                     RRGGIEGLLSANYPGLIQAIDTKRADEFQAAFGKLYASCVTCHVNHGVPFYPLIPTTS
                     ISPITSGSIELWQEIQKHLRQEQEKKIQQEREKK"
     gene            1640..2884
                     /locus_tag="VI935_06050"
     CDS             1640..2884
                     /locus_tag="VI935_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HLE25197.1"
                     /translation="MDSIRLLLGVLVNVFLIGASTEYTSVKKEGYTESQTCGACHTVI
                     YKEWKNSLHASSISDPVFLGVFNEVEREKKNFCLKCHAPTVRVTGDYDLTQFITKEGV
                     TCDFCHTVKSVDLSKEEPFEFDPGFVKRGPFESDLEIGHKNLYSEIHLKAEFCAGCHE
                     VVNENGFHVMSTYSEWKGGPYAEKGIQCQNCHMPEEFGIPIVNTEIAKTQRSVTAHKF
                     LGGHSQINITRAATLTQILDKADDSLSVIVYVTNSEAGHSLPTGIPSRNVILTVKLLD
                     TKGGVVREKEVVYRRVLVDEEGKEIPEENIKDMFLKATSLKSDNRIKPKETRREEFIF
                     PLAKGIEKVLTVDSTLRYEFSVPYLEPNIMRMEMARDVKTINIEEEGAVIWKWTIALV
                     AVLVLLFILQITLRKLRRHPGG"
     gene            2877..3320
                     /locus_tag="VI935_06055"
     CDS             2877..3320
                     /locus_tag="VI935_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HLE25198.1"
                     /translation="MDEKIGRRNLLGTLVGWIFGLTVVGCIIPIIAYLFPKSKGEAEN
                     IFMDSSGKPITVSAILKEGSKTGLAFGDATVVISYGGEVRAFSAVCTHLGCIVSWKKD
                     EGIFFCPCHAGKFDSNGNVISGPPPKPLLRYGVKVEGRKINLSRV"
     gene            3322..>3676
                     /locus_tag="VI935_06060"
     CDS             3322..>3676
                     /locus_tag="VI935_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HLE25199.1"
                     /translation="MLKKIIDWIDERVQIIEVIENHLTGHMVPANLNFWYSMGSVLLA
                     MLFIQFITGFLLLIYYVPDVDKAFESVAYITNEVPFGWLIRRVHAMATNIFIAVLFLH
                     MFSTLFMGSYKKPREL"
BASE COUNT         1139 a          633 c          849 g         1055 t
ORIGIN      
        1 atactgtcag attttctaac atcagacagt ttgtaatcca tacctgcttt gtaattccca
       61 ctaaatatta caaaaacatt aaatgttatt ttggcatgta acttgccatt attgaagtta
      121 taaagggagg aggtgaaaca aaatgacatg gggagatatt attagttttt tctttattct
      181 tgttcttaat ccatatggtg tagtgggaat agctattgct ctcttaatgg tggcaactgc
      241 atttattttc ttcggcgtag aggctattac tgtaccttac cgttatgtag taaaaaggtt
      301 ttactgtccg ttgaggaaga cgaatgttga actgaaactc agaccaagca tcttcacata
      361 tagagcgtac gatgatgtta ttaagtgttc tgcatttaaa ggcaaagtca cctgcaagaa
      421 gggctgctta gatcttccag agcttcgaac ttgagagtag aaagggaaaa taaacatact
      481 acgaaggcta aaaaaatatc ccgaagaaaa taatattaga catgttgcga aataggattt
      541 tcgcgttttt cgagccttaa aatcaacggc ttagaagtca gatttttaat ttcgcaacaa
      601 atcttttata aataggattt atacagtgag ggtgaggcta tagcatattt cagttgggta
      661 cgatacagaa agcggctttc ggtagagtct gtcattcccg cacgcattaa gcgggaatcc
      721 agttgtatat ggatacccga taaaagcatt cgggtatgac aaatacacca tgctgtactg
      781 aatgcaaatg aaatacgcta taggagggtt tattgtattt tttaagataa attcttgaag
      841 tctaactagg tctttgttat gattcctacc aaattgaacc aaggaggatt agcagaatga
      901 gaaaaatgat tctagttctg gtggtgattt cttcattagc tacggtagga atattggcta
      961 tcatggcggc ggatcccggc cgttataggc ctccaacacc ttcctggttt actgggtcaa
     1021 ccgaagataa gatgcaaaaa cttgggctca ttcagccctc ttattccgac cttatgctgt
     1081 ggactggaat ccgccttaat gagttatttg cagcggggac tagcggaaaa acagagtatg
     1141 caaagcttca agcaaaaaaa atcgaggaaa ccctggaaaa ggccgcattg gttgatccca
     1201 caagaagagg aggaatagag ggacttcttt ctgcgaatta tccgggtctc atacaagcga
     1261 tagatacgaa aagagccgat gaatttcaag ctgcctttgg aaaactttac gctagttgtg
     1321 ttacctgtca tgtaaaccat ggagtcccct tttatccgtt aattcctaca acatctataa
     1381 gccctataac atcaggttct atagaactat ggcaggaaat ccagaagcat ctgcggcagg
     1441 aacaggaaaa gaaaatacag caggaacggg aaaaaaagta acggagggag caaaaagtag
     1501 tttgactact ctgaatagtg tgtgtaaagg gttaggcatt atcgacgaag ttgaatgccc
     1561 gacctctacc cgcatttctt tcaatgaaat cgaatgtgat ttagaaacaa taatccgagt
     1621 tattgaagct taaatacaaa tggattctat aaggctgctc cttggtgttt tggtaaatgt
     1681 cttccttata ggagcctcga cggaatacac cagtgtaaaa aaggaaggct atacagaaag
     1741 tcaaacctgt ggtgcatgcc acacggttat atataaagag tggaagaatt ctctccacgc
     1801 ttcttccatt agcgaccccg tcttccttgg tgtttttaac gaggtagaga gggaaaagaa
     1861 aaacttttgt cttaaatgcc atgctcctac tgtaagagtt acaggggatt atgatcttac
     1921 ccagtttata acaaaagaag gggtaacatg tgacttttgt cacactgtaa aaagcgtaga
     1981 tttaagtaaa gaagagccat ttgaatttga tccagggttt gtgaaaaggg gtccatttga
     2041 aagtgactta gaaataggcc ataagaatct ttactctgaa attcacctaa aggctgaatt
     2101 ctgcgccgga tgtcatgagg tcgtgaacga aaatggattt catgtgatga gtacctacag
     2161 cgaatggaaa gggggtcctt atgcggaaaa ggggattcaa tgtcagaact gtcatatgcc
     2221 cgaggagttc ggtattccta ttgttaacac tgaaattgca aagacccagc gtagtgtaac
     2281 agcgcataaa tttctagggg gacactctca gataaatata actagggctg caacgctaac
     2341 tcagatctta gataaagccg atgactctct atccgttata gtgtatgtaa ccaactcgga
     2401 agcagggcat tccctcccta caggcattcc ctcacgtaat gtaatactta cagtaaagct
     2461 tctggataca aagggagggg tagttagaga aaaggaggtt gtatataggc gggttcttgt
     2521 tgatgaagag ggaaaagaga tcccagagga gaatattaag gatatgtttc taaaagctac
     2581 gtccctaaag agtgacaacc gcatcaaacc taaggaaaca cgcagggagg agtttatctt
     2641 tccacttgct aagggaatag aaaaggtttt aacagtagac tctaccttaa gatatgaatt
     2701 tagtgttcct tatctcgaac caaatataat gagaatggaa atggctcgcg atgtgaaaac
     2761 aataaacatc gaggaagaag gggctgttat atggaaatgg actattgccc ttgtagcagt
     2821 tttagtgctt ttatttatcc tgcaaataac attacgaaag ctaaggaggc accccggtgg
     2881 atgagaaaat cggaagaagg aatttattgg gaacgttagt tggttggata ttcggtctta
     2941 cagtagtagg ctgtataatc cctataatag catacctttt cccaaaatct aagggggagg
     3001 cagaaaacat ctttatggac tcttcgggta agcctataac ggtgtcggct atattaaaag
     3061 aggggtctaa aacagggctt gcttttggag atgctacagt tgttattagt tacgggggag
     3121 aggtcagggc tttttcagca gtgtgtactc accttggttg tattgtaagt tggaaaaagg
     3181 atgaaggcat tttcttctgt ccctgtcatg cggggaaatt tgactcgaat gggaacgtta
     3241 tttcaggacc gcctcctaaa ccgcttctca ggtacggggt taaggttgag ggacgtaaga
     3301 taaacctttc aagggtgtaa tatgttaaag aaaatcatag attggattga tgagagggta
     3361 caaattatag aggttataga gaatcatctt actgggcata tggttcctgc taacctaaat
     3421 ttttggtact ctatgggctc tgtacttctt gcaatgcttt ttattcagtt tataaccggt
     3481 tttttgcttc ttatatatta tgtgcctgat gtagataaag cctttgaaag cgtcgcctat
     3541 ataacgaatg aggttccgtt tggatggtta ataagaaggg ttcatgctat ggcaacgaac
     3601 atattcatag ctgtgctctt ccttcatatg tttagtaccc ttttcatggg gtcttataag
     3661 aagccgaggg aacttc
//
LOCUS       DATDRL010000174         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2942651, whole genome shotgun sequence.
ACCESSION   DATDRL010000174 DATDRL010000000
VERSION     DATDRL010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2942651"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            609..1580
                     /locus_tag="VI935_06065"
     CDS             609..1580
                     /locus_tag="VI935_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012324565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HLE25200.1"
                     /translation="MGYGSRGESMQSGSTRSEEPIQPIPISFNYDRPKIELGKKLFFE
                     PRLSKSGWISCNSCHNLSTGGADNLPSSIGHKWLFGPINSPTVLNAKFNLAQFWDGRA
                     KDLKEQARGPIANPMEMASNHELAVGILLSIPEYVGWFKEVYKDEKITIDNVADAIAA
                     FEETLTTPNSRFDWWLKDYDDKISEFEKEGYDLFKAKGCINCHNGFGVGGNSFQKFGI
                     AKPYDKDTQTLGRYNVTKDEKDKYVFKVPLLRNIELTAPYFHDASTWNLSEAVNIMAE
                     YQLGVPLSRDETNKIVAFLKTLTGDQPSIILPTLPPSTANTQKPDRN"
     gene            complement(1618..2064)
                     /locus_tag="VI935_06070"
     CDS             complement(1618..2064)
                     /locus_tag="VI935_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HLE25201.1"
                     /translation="MYKIVLDSTVLVSAFLAKTGVSAKLLHKAKDGGFDICLAEEILE
                     EVQRALLQYQKIRKRYHYSDQTVIQFVQNLRIVAHLITELPSIEVVNSDPNDNMVVAC
                     ALKAQADYIVTRDNDLLSLENYEDIKVVTPEKFMGILRSGKHKPPF"
BASE COUNT          641 a          444 c          467 g          663 t
ORIGIN      
        1 ccgtcatgtg aggtttgtct tttcgtatgg atacctgtaa tcgatattgg gatttgggcg
       61 tttccgtgaa tgcctcatac aaatttcttg atgcagagtg tcgcacggaa cactcgatca
      121 tgcactgttt gcacgtcacg catttgtctt tgacgaattt ctttttcaac atttgcagat
      181 gctccttgag tttgtgagat aaataagtaa aataatttta taataaatct caacaagtcg
      241 caaccaaaaa agatgaagcg ttcgagatca agttattctt tttaagtccc ccttaacaaa
      301 gggggattca agggggttgt gtttttccgt ggttatttta caaccccctt tgcccccttt
      361 tcgttcgact gagcgctcac gacgaagtct aagggggatt tatgcatcgt atatgttatg
      421 tgagccgtat aaataaaatg agaattccta taagataaac agtaaataac aagtacacac
      481 tttcatcatt cacagaaaaa ggggggcaaa tgaagatttt taaatttact gcgattagct
      541 gtttattggc ttttgccgtt atcaatggcg tgatgccggc atttactatc gcaggaggtc
      601 tgattgatat gggttatggc agtagaggtg agagtatgca gtctggatca acacgttcag
      661 aagaacctat ccagccaatt ccaatcagct ttaactacga cagaccaaag atagaattag
      721 gaaagaaatt gttttttgaa cccagattgt ccaaatccgg ctggatcagt tgtaattcct
      781 gtcataacct ctcaacaggc ggcgcagata atttaccaag ctctatcggg cataaatggc
      841 tctttggtcc gataaactcg ccgaccgtct tgaatgcaaa attcaatctg gcgcaattct
      901 gggatggacg cgcaaaggac ttgaaagaac aggcaagagg ccctatagcc aatcctatgg
      961 aaatggcttc aaaccatgag cttgccgtag gtatattgct atcaataccg gaatatgtgg
     1021 gatggtttaa agaggtgtac aaagacgaga aaattaccat tgataacgtc gctgatgcga
     1081 ttgcggcttt tgaagagact ttaaccacac caaactcaag atttgattgg tggctaaaag
     1141 actatgatga caaaatttcc gaatttgaaa aggaaggata cgatctattt aaggccaaag
     1201 gttgtataaa ctgccataac ggttttggcg tgggaggaaa ttcttttcag aaatttggca
     1261 ttgcaaaacc atatgataaa gatacccaga cccttggcag atacaatgtg acaaaagatg
     1321 agaaagataa atatgtgttt aaagtacccc tgctgaggaa tattgaacta acggcacctt
     1381 atttccacga tgcaagcaca tggaatttga gtgaggcagt gaatatcatg gcagaatatc
     1441 aactgggagt accactttct agggatgaaa cgaacaagat tgtcgctttt ctcaagacct
     1501 taaccggaga ccaaccttca attattcttc caacgctgcc accttcaact gcgaataccc
     1561 agaagccaga tcggaattga ttttgtgctg ttgttctgtt ccttctatga aatggattta
     1621 gaagggaggt ttatgcttcc cactccttag tatccccata aacttctcag gtgtcactac
     1681 ctttatatcc tcgtagtttt cgagagaaag aaggtcattg tctcgggtaa ctatgtagtc
     1741 cgcctgtgct ttaagagcgc acgcaaccac catattatca ttagggtcgc tattgactac
     1801 ctcgatggaa ggtagctctg tgataaggtg tgccactatc ctgagatttt gaacgaattg
     1861 aattacggtt tgatctgagt aatggtagcg ttttctaatc ttctggtatt gcaaaagagc
     1921 ccgctggact tcttcaagaa tttcttctgc caggcatata tcaaaacctc cgtctttagc
     1981 tttgtgaagt aatttagcag aaacacccgt tttagcaaga aaagcgctaa caagaaccgt
     2041 tgaatcaagg acaattttat acatgttgtt tcctaaaacc cttcaccatt ttggcaattt
     2101 cctcttcctt ttttttagcg ctctgctttg gtttcggttt gcgatccttg acgttttcca
     2161 tagatttaaa aacttgattc catgccttct ctcgctccac cacagttact ggttt
//
LOCUS       DATDRL010000175         2775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3791601, whole genome shotgun sequence.
ACCESSION   DATDRL010000175 DATDRL010000000
VERSION     DATDRL010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3791601"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /gene="thrC"
                     /locus_tag="VI935_06075"
     CDS             <1..1241
                     /gene="thrC"
                     /locus_tag="VI935_06075"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HLE25202.1"
                     /translation="KGLRCRECGKEYPKAPIHVCEFCFGPLEVTYEYDEIKRVFSRDV
                     IKSRSPDIWRYAELLPLDKPNSVGKRVGFTPLLKADNLARVLGVREIYVKNDAVCYPT
                     LSFKDRVVSVALSKAKEFGYKTVACASTGNLANAVAALSALGRLESYLFIPYDLEQSK
                     IIGTTVYGAKLIGINGSYDDVNRLCSEIAGKFGWAFVNINIRPYYAEGSKTYGFEIME
                     QLGWRLPRNIVVPMASGSLITKIWKAIQEFDKLGLVDRTETKIYGAQATGCSPITTAL
                     KNGWEIFKPVKPNTIAKSLAIGNPADGYYAMKVMKESGGCGEDVTDEEVVEGMMLLAE
                     TEGIFTETAGGVTMAVTKKLIENDIIPRDESIVVSITGNGLKTLEPLYDKVSKPHIIG
                     ARLEEFEDLYSRLNSREKRR"
     gene            1247..1516
                     /locus_tag="VI935_06080"
                     /pseudo
     CDS             1247..1516
                     /locus_tag="VI935_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196494.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
     gene            1631..2539
                     /locus_tag="VI935_06085"
     CDS             1631..2539
                     /locus_tag="VI935_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLE25203.1"
                     /translation="MSRLDVVGIGQVCIDYLGKVRYFPDFEGKVEFEDVTIRGGGPTA
                     TALLVLARLGLKVAIIGKVGDDDLGRILIQDLKNEGIDTSGLIKEKSKKTQLSIIPIE
                     KKSGRRTVFWSKGTITPLKSKEIKKELIAHARAIHFDELFMEANVEAANIAKKLGITV
                     SMDAGNYIDGINELKGKVDLFIASQDFMREYTGENNPTNGIRKLKEFKAKVTTVTLGS
                     KGSLTIYNDELIKTPAYKVNAVDTTGAGDVFHGAFLYGWLKNWEIKKALDFASAAAAL
                     NCTYLGAQGGIPKNTKDIFDFMRMCK"
     gene            complement(2568..>2775)
                     /locus_tag="VI935_06090"
     CDS             complement(2568..>2775)
                     /locus_tag="VI935_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HLE25204.1"
                     /translation="QSFRLKGKGVASLNGKGKGDLYIRVQVEVPVKLTSKQRQLLEEF
                     ARSGEGDENPAVKKFFEKFRELFG"
BASE COUNT          931 a          493 c          628 g          723 t
ORIGIN      
        1 taaaaggact aagatgcaga gagtgcggga aggaatatcc aaaggcacct attcatgtct
       61 gtgagttttg ctttggccca cttgaggtaa catacgaata tgatgaaata aaaagagttt
      121 tttctcggga tgtaataaaa agcaggtccc ccgatatctg gcgttatgcg gagcttttac
      181 cccttgataa accaaatagc gttggaaaac gcgtagggtt tacgccactt ctaaaagctg
      241 ataacctggc aagggtatta ggtgttagag agatttatgt aaaaaacgat gccgtatgct
      301 accctaccct ctcattcaaa gatagggttg tttcagtggc actctcaaag gcgaaggaat
      361 ttgggtataa gaccgttgcg tgtgcttcca ctggaaactt ggcaaatgca gttgcagcgc
      421 tctctgcatt aggaaggctt gaaagctatt tattcattcc gtatgaccta gaacaatcaa
      481 agataattgg aaccactgtt tatggagcaa agcttatagg gattaatggc agttatgacg
      541 atgtaaatcg tctctgtagc gagattgcag gaaaattcgg ctgggcattt gttaatatta
      601 atatacgccc atattatgct gagggatcca agacttatgg ttttgaaata atggagcaac
      661 ttggatggcg cttaccaaga aacattgtag taccgatggc aagcggctct ctgatcacaa
      721 aaatatggaa ggctattcag gaatttgata aactaggttt ggtagataga acagaaacaa
      781 agatatatgg ggcgcaagca acaggatgtt ctcctatcac tactgcatta aaaaatggtt
      841 gggagatttt taaacctgta aaaccaaata caattgcaaa atcccttgct attggcaacc
      901 cagctgatgg atactacgca atgaaggtaa tgaaagaaag tggcgggtgt ggagaagacg
      961 ttacagatga agaggttgtt gagggaatga tgcttcttgc agagaccgag gggatattta
     1021 cggaaacagc aggtggtgta acaatggcag tgactaaaaa acttatcgaa aacgatatta
     1081 taccgagaga tgaatcaatc gttgtgtcaa ttactggaaa cgggctcaaa accctggagc
     1141 ctctttatga taaggtatca aaaccccata tcattggagc aaggctagag gagtttgaag
     1201 acttatatag tagattaaac agccgagaaa aaaggaggta actgaaatgc cgtctgtaag
     1261 aatacccact ccacttagaa gacttacggg agataaagat gaggtaacaa taaacgctaa
     1321 gaatatccga gaactcatag aagggcttga aaaacagttc cctggggtga aagaacggct
     1381 ctgcgatgaa agtggaaatg taagaagatt catcaatcta tacgttaata acgaagacat
     1441 aagatttcta aaggggattg aaacggagct aaaggaaagt gacattgtat caataattcc
     1501 agcaattgct ggtggtatga aacaatacga gccacgaatg aacacatatg agcacgaatt
     1561 atttattttt gctaaatata aaatctttat tcgtgaaaat tcgcgtgtat tcgtggctaa
     1621 ataaaatctt atgagccggc ttgacgtagt tggaatcggt caggtctgta tagactacct
     1681 cgggaaggtt cgttatttcc ccgactttga aggaaaggta gaatttgagg atgttactat
     1741 tagaggagga ggacctacag cgactgcgct tttggtcctt gctcgtttag gattaaaagt
     1801 cgctattata gggaaagtag gagatgacga tcttggaaga atcttgatcc aagaccttaa
     1861 aaacgaagga atagacacct ctggtttaat caaagaaaaa tcaaaaaaaa cccagctttc
     1921 aattattcca atcgagaaaa aaagtggcag gcgaacggtc ttctggtcaa aaggaactat
     1981 cactccactt aaatcaaaag aaataaagaa ggaactcata gctcatgcaa gggcaattca
     2041 ctttgatgag ctattcatgg aagctaacgt cgaggctgcc aacatcgcaa agaaattagg
     2101 aattacagtc tccatggatg caggtaacta tattgatggg attaacgaac tgaaaggtaa
     2161 agttgatttg tttatagctt cccaagactt tatgagagag tacacaggag aaaataaccc
     2221 tactaacgga ataagaaagc ttaaagagtt taaggcaaag gttacaacag taacacttgg
     2281 ctctaaaggc tcacttacaa tttataatga tgaacttatt aaaaccccgg catacaaagt
     2341 taacgccgta gatacaacag gagccggaga cgtattccac ggagcgtttc tttatggatg
     2401 gctcaaaaat tgggagataa aaaaggcctt ggatttcgct tcagccgccg ctgcactgaa
     2461 ctgcacttat ctaggagcac aaggtggaat ccctaagaat acaaaagata tatttgattt
     2521 tatgagaatg tgtaaatagg agatctagac gctaagtccc aactttatta tccaaataac
     2581 tcacggaatt tttcaaaaaa ctttttgact gcgggattct catcaccttc gcccgatctt
     2641 gcaaactcct caagtagctg cctctgcttt gaagtcagtt taactggaac ctctacttgt
     2701 acccttatgt aaaggtcacc ctttcctttt ccattaaggc tggctacacc cttaccctta
     2761 agtctgaagg attgt
//
LOCUS       DATDRL010000176         5005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2699126, whole genome shotgun sequence.
ACCESSION   DATDRL010000176 DATDRL010000000
VERSION     DATDRL010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2699126"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VI935_06095"
     CDS             <1..458
                     /locus_tag="VI935_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25205.1"
                     /translation="VKLNGPTQNLGKHQLLTASFEFTDIIKNKQPVSLIRELTYEVTQ
                     DKTKVAANENKVVAARGVSVDAASELYRATMYYEQGNRDDALSNIKRALGMIVQLNSS
                     PQKSAATVKQEAELRDAMEEMSAVPSAPESDAGKKLIKEYKAKSREQQK"
     gene            571..1185
                     /locus_tag="VI935_06100"
     CDS             571..1185
                     /locus_tag="VI935_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25206.1"
                     /translation="MKNKFKSLLIPILLGGAWGVFTYLVGSLVLSGFSLASFLFYPIL
                     FGGVTGIIAYTFESRIPFARAIAIGLISGLIYHILSPISPFLTSVFVGASFGGSLATD
                     VGKLKDVFRRLISILKGVFIFPFFIYVGGLLAGLTSSVFGSNFIIWFFWGGWLSLGIR
                     IINMPLFKEANSSEDFQTLSEADEFTSETHEILRELRQLDSRFK"
     gene            1347..2027
                     /locus_tag="VI935_06105"
     CDS             1347..2027
                     /locus_tag="VI935_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25207.1"
                     /translation="MRNITKVALFFLVVCIVFGFSKSTEAQTNTQPEVNLPKKPEVQP
                     NPMRGICAYMWDEYYEKEKIEINVSTRGDYDETLIFTCPLCSYRSHLVDPFLNTEYQG
                     KTGMDRVVECGFTQVVFRGGRGMEEIVVQVPKVKPDPVRSTCAKTWNAYYENENSDIR
                     VSTRGDYDETIVFACSYCNLENQFVNPFLNNEYQGKSGIARIKECGFIEAVFEGERGV
                     EPIVVQVP"
     gene            2033..2263
                     /locus_tag="VI935_06110"
     CDS             2033..2263
                     /locus_tag="VI935_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25208.1"
                     /translation="MVKNLNIVTFPFGWIREYEDDNWQLIWSSETKILYAKGAITKKV
                     VSIGQPSTWQEAKALATRVQNEPEVYISLAED"
     gene            complement(2352..3035)
                     /locus_tag="VI935_06115"
     CDS             complement(2352..3035)
                     /locus_tag="VI935_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HLE25209.1"
                     /translation="MISIVIPTYNESSTIEGTLINLFEIISTGDEVIVVDGFSEDDTK
                     EIAGKFTKVKLFNSKRGRATQMNSGAEKTNNEYILFLHADTTISATCLTMLKNEIISN
                     RIGWGWFPIKLNSPKFIFRVLELGANMRTSFTGTPLGDHGIFVKKDLFFEVGGFPDMP
                     IMEEIEFVRKLKKISNGVEIQSSVRTSVRRFERSGVLKTFVTMWILRILYYLGIPTKT
                     LLRYYSHIR"
     gene            3314..4000
                     /locus_tag="VI935_06120"
     CDS             3314..4000
                     /locus_tag="VI935_06120"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HLE25210.1"
                     /translation="MINTMREVNKKKILLILGVVVILVAVFLLVRDSGIEFEDLTPSK
                     IREYLLSFGMVKGAFVYLAIYTFSIRPFIPIPPTLYTLAGGFTFGPLWGSLLTVVGAT
                     LNASLCFLIARALGKDFVEKVSKGKFDKLNERLRNSGFKTLLIIRTSPVGPPFDLVSY
                     ASGILRISFWSYVVATMVGVIPAVAVYSYFGGSITKGGLTILIAFFLVVVISIFVPWY
                     IKRRKQPQIH"
     gene            complement(4027..4326)
                     /locus_tag="VI935_06125"
     CDS             complement(4027..4326)
                     /locus_tag="VI935_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25211.1"
                     /translation="MEEILNKIMSDLEILKEQLKDISSTAHSNAERIKRNGELIERNY
                     KQPLQNERIIKQNRELISRNKTLIEQNGRILKQILARLQRVEAKAGAAVTKRRKK"
     gene            4512..>5005
                     /locus_tag="VI935_06130"
     CDS             4512..>5005
                     /locus_tag="VI935_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25212.1"
                     /translation="MPSEKINFLLESYKDTENLIRLCDAKAAGIITILAFMFLPLVPS
                     LKASVHRIFEKGLVGWELIVGAVVVILILGFLGSVLLTFSFMFKAVKPRKKALEHSFE
                     TKNVFWCLDICSIVDKHGADHFKDLVDQIDNFDNELMQAVIIISYIGKEKLLAIDKAT
                     KYLK"
BASE COUNT         1493 a          838 c         1024 g         1650 t
ORIGIN      
        1 tagttaagct aaacggacca acccaaaatc ttggaaaaca ccagcttcta actgcttcat
       61 ttgaatttac agatattatc aaaaacaaac aacccgttag tttgataaga gaactaacct
      121 acgaggtcac acaggataaa accaaagtag cggctaatga gaataaagtt gtcgctgcaa
      181 gaggggtatc ggtggatgcc gcatcagaat tataccgagc gactatgtac tacgagcaag
      241 gaaatcgtga tgatgcttta agcaatatta aaagagccct ggggatgatc gttcaactta
      301 acagctctcc tcaaaagagc gcagctactg taaagcagga agcagaactt cgggacgcaa
      361 tggaggagat gtctgcagtg ccctcggctc ccgagtccga tgcgggaaag aagctgatta
      421 aggaatacaa ggctaagtca agagaacaac agaagtagaa tgctgtggtt cgtgattcgt
      481 ggctcgtgaa caatgatttt tgaatcgctt atgagacatc gaaccacgag tcatgataca
      541 ctattcccgc atgcaaatgg agaataaatc atgaaaaaca aattcaaatc actattaatt
      601 ccaatactcc tcggtggagc ttggggagtc tttacctacc ttgtcggttc tctcgtatta
      661 tccggatttt ccctcgcatc tttccttttt tatccaattc tttttggagg tgttactggt
      721 attatcgctt acacttttga gtcaagaatt ccgtttgcaa gggctatagc tataggcctt
      781 atttcagggc ttatttatca catcttgtct cccatttctc ctttccttac atcagttttt
      841 gtaggagcaa gctttggtgg tagcttggca accgatgtag gaaagttaaa agatgtgttt
      901 aggaggctta tatctattct aaaaggtgtt tttatctttc cattttttat ttatgtagga
      961 ggcttacttg caggtcttac gagcagcgtc tttggctcga attttattat ctggtttttc
     1021 tggggaggat ggcttagttt gggaatccgc attattaata tgcctctatt caaagaagcg
     1081 aacagcagtg aggactttca aactctttcc gaggctgatg aattcacatc tgaaactcat
     1141 gaaattctta gggagttaag acagttagac tcgagattta aataagcagt cttcaacaga
     1201 gtataaattg atattgaaaa ataattctgg gttgtcacat tgttgatttt tgccaaatct
     1261 taacctaata gttaaactaa acgtataata acgatatatt tcagtttaaa aatgtagtaa
     1321 aattagaaca actaggagga gaagagatga gaaatataac caaagtagca ttattttttt
     1381 tggtggtttg tatcgttttt ggattcagta aatcaactga agctcaaact aatacacaac
     1441 ccgaagtgaa tcttcctaaa aaaccagagg ttcaacccaa tcctatgaga ggtatttgtg
     1501 cttatatgtg ggatgagtat tacgaaaaag aaaaaatcga aataaatgtc tcaacaagag
     1561 gagattatga tgagactctg atatttacct gtcccctttg tagttatcgc agtcatttag
     1621 tagatccatt tctgaatact gagtatcagg gtaaaaccgg tatggatagg gtagtagagt
     1681 gtggttttac tcaggttgtc tttagaggtg gaagaggaat ggaagaaatc gtagttcagg
     1741 ttccaaaggt taagcccgac ccagttagaa gtacctgtgc taagacgtgg aatgcatact
     1801 atgagaatga aaatagtgat ataagggttt caacaagagg agattatgat gagaccattg
     1861 tgtttgcatg ttcctattgt aatcttgaga atcaatttgt taacccattc ctgaataacg
     1921 aataccaggg caaaagcgga atagccagaa taaaggagtg cggttttatt gaggctgttt
     1981 ttgaagggga aagaggggta gaacctatag tagtgcaggt tccataaaaa ggatggtgaa
     2041 gaatttaaat attgtgacgt ttccatttgg ttggattcgt gagtacgaag atgataactg
     2101 gcagctaata tggagttctg aaactaagat tttatatgct aagggtgcta ttacgaagaa
     2161 ggttgttagt attggacagc cttcaacttg gcaggaggca aaagcccttg ctacaagagt
     2221 acagaatgaa cctgaagttt atataagcct agcagaagat tagttcgagg gaaaagaatc
     2281 tatgtgaaaa cctttacgat ctttactgca ctaataatcg ggcctttgta cttactaagt
     2341 tgaataagca ttcatctaat atggctgtaa tatcttaaaa gtgttttggt tgggattccc
     2401 aaatagtata gtattcttaa aatccacatg gttacaaatg tctttaaaac tccagacctt
     2461 tcgaatcggc gaacgctcgt tctgactgat gattgaattt caactccatt tgatattttt
     2521 ttaagcttcc ttacaaattc aatttcttcc atgattggca tatctggaaa tccaccaact
     2581 tcaaaaaata aatctttttt aacaaatatg ccgtgatccc caagaggggt tcctgtaaaa
     2641 cttgtcctca tgtttgcccc taattctaaa accctaaata taaacttagg ggagttcaac
     2701 tttattggaa accatcccca cccaattctg tttgatatga tttcattttt taacatagtt
     2761 aagcacgttg cacttattgt tgtgtcggcg tgaagaaaga ggatatattc attattggtc
     2821 ttttctgcac ctgaattcat ctgtgttgcc cgtcctcttt tggagttaaa gagttttact
     2881 ttagtaaact tgcctgcgat ctcctttgta tcatcctcac tgaagccatc aactacgatc
     2941 acttcatcac ccgttgatat tatttcaaaa aggtttatga gtgtgccttc gatagttgag
     3001 gactcgttat atgtgggaat tacaatgctt atcatcaggc tatgtatgag ttatacttaa
     3061 atataactta tatattgagt taattttatc tgtattttct caaatggaat ccttcttatc
     3121 gtcgcaaacg tctaaatgaa tatctgttgt ttagaataaa taatgactct atttttttat
     3181 atggtgtgta aagaggcagt cagtctaata tatgcgcgaa tcttctccta atgggcatgg
     3241 aaggaagtga tgccctattc aaaacatatg attaatctat ttactagtaa ggtaggaggt
     3301 tgaatctttg tatatgatta atacaatgag agaggtgaat aaaaagaaaa tattactaat
     3361 acttggagtg gttgtaattt tagttgcagt ctttttgtta gtaagagact cgggtattga
     3421 gtttgaggat ttgacacctt caaagatcag ggaatatctt ctgagcttcg gaatggtgaa
     3481 gggggcgttt gtttatcttg caatttacac attttctata agaccattta ttccaatacc
     3541 accaacactc tatacgcttg cagggggttt cacattcgga cccctctggg gatctctact
     3601 taccgttgta ggtgcaacgc taaatgcaag cctttgcttt cttatagcaa gggcgctggg
     3661 taaggatttt gttgagaaag tatctaaggg gaaatttgat aaacttaatg agaggttaag
     3721 aaatagcggt tttaaaacgc ttcttataat cagaacttca cctgtaggtc ctccattcga
     3781 tttggtcagt tacgcttctg gtattttaag aatctctttc tggagttatg ttgtcgctac
     3841 aatggttggt gttataccag cagttgctgt gtactcatat tttggcggtt caatcacaaa
     3901 aggtggtctt acgatactaa ttgccttttt cttggttgtc gttatctcaa ttttcgtgcc
     3961 ttggtatata aagcggagaa aacaaccgca aatacattaa ttatcctttg taaaacaaag
     4021 aagaagttat ttttttctac gtttagtaac agcagctcct gctttagctt cgactcgttg
     4081 gaggcgggct agaatctgtt ttaaaatcct tccgttttgt tctattagtg ttttgtttct
     4141 actaataagc tctctattct gcttgataat cctctcattc tgaagaggtt gtttataatt
     4201 tcgctcgata agttccccat tgcgctttat acgctcagca ttactatgag cggtagaact
     4261 tatatctttt aactgttctt ttagaatctc caaatccgac ataatcttat ttaaaatctc
     4321 ttccatgcgg ccctccttac ttgtatttta aaagctgaaa atcctgtatt ttgtcgttag
     4381 attactgttt catatttcta ttgtcaagtc cagggagtaa ataggagggt ttcaaagctt
     4441 cattgctaat gaattgttca atagtatgaa gcaaaagtct tccatttctg ttaaattgat
     4501 taagaaacta catgcctagc gaaaagataa atttcctttt agaaagttat aaggataccg
     4561 aaaatctgat tcgtctttgc gatgcgaagg ctgcaggaat tattactatc ttagccttca
     4621 tgtttctccc gttagtccct tctttgaaag caagtgtaca tagaatcttt gaaaaaggct
     4681 tggtcggctg ggagttaata gttggggctg tcgtagtgat acttatactt ggttttcttg
     4741 gctctgtttt actaacattt tcttttatgt ttaaggcggt gaaacctagg aaaaaggctt
     4801 tagagcatag ttttgagacc aaaaatgtat tctggtgtct tgatatttgc agtatagtag
     4861 ataagcatgg ggctgaccac tttaaggatt tagttgatca aatagataac tttgataatg
     4921 agttaatgca ggcggtaatt attatctctt atataggaaa ggagaaattg cttgctattg
     4981 ataaagctac aaaatatttg aagta
//
LOCUS       DATDRL010000177         5157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3279537, whole genome shotgun sequence.
ACCESSION   DATDRL010000177 DATDRL010000000
VERSION     DATDRL010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3279537"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VI935_06135"
     CDS             <1..183
                     /locus_tag="VI935_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HLE25213.1"
                     /translation="FCRHYKHQWGSNTVRYASCGIKQPRQMRRPMLSNRFTTNWDEIL
                     KVKASFPIKFDSEAST"
     gene            239..385
                     /locus_tag="VI935_06140"
     CDS             239..385
                     /locus_tag="VI935_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25214.1"
                     /translation="MEEFKAIYDEKEDWLYLTKTNGSVHVEDTEIGKGIYPKKCTYPI
                     STVT"
     gene            complement(502..675)
                     /locus_tag="VI935_06145"
     CDS             complement(502..675)
                     /locus_tag="VI935_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HLE25215.1"
                     /translation="MTKEIRCRDVGVDCDWVASGETEEEIMKKVAEHAKKDHGMDEIP
                     EELVQRVRAAIHT"
     gene            complement(1122..1322)
                     /locus_tag="VI935_06150"
     CDS             complement(1122..1322)
                     /locus_tag="VI935_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25216.1"
                     /translation="MPFKVRAIQVDRGSEFFREFEQECQGRGINLFVLPPKSPKLNGC
                     VERAQRTHTEEFYEVSECSWTE"
     gene            complement(1340..1918)
                     /locus_tag="VI935_06155"
     CDS             complement(1340..1918)
                     /locus_tag="VI935_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25217.1"
                     /translation="MTNWDRVSRTNYRSKAFWDVHWIMKARDLLESAKLIEPEVVRYW
                     DNSREVLKTKSGQFLPDYYLGTYFMLIAYAVENIFKAVIVRNNSVDYKEKFKQTPKFP
                     KELQSHSLVELAKQAGYSFNLYEEDLLRRLTRSAIWYGRYPVPLDYKFSSNTEIFSDG
                     KKRSVGWFGANYIPRLKKFIDDLKVSLNISGE"
     gene            complement(1985..2257)
                     /locus_tag="VI935_06160"
     CDS             complement(1985..2257)
                     /locus_tag="VI935_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HLE25218.1"
                     /translation="MQRLLHAEDPKAYTILNKLGNSVAYKRGGRLLAYVYLPDGTMLN
                     TLLVQEEYAQVMTVFPKVKYQDMFLKLQREARDNKRIVEKGNVNAR"
     gene            complement(2208..2444)
                     /locus_tag="VI935_06165"
     CDS             complement(2208..2444)
                     /locus_tag="VI935_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25219.1"
                     /translation="MSQILLDLHEKAQQETKQMLLDAQRQTRLQLEHLDRHSKQLVEQ
                     RIKGEQWLGKKYPNGYELIAKVVTCRRPQGLHNT"
     gene            complement(2932..3723)
                     /locus_tag="VI935_06170"
     CDS             complement(2932..3723)
                     /locus_tag="VI935_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25220.1"
                     /translation="MKKIITMQRCIHLFALCFSLFLILWGVGCNGGGGGGGCPVPTLD
                     IEVCDPETGGPFSLEIDNDFFPVVPGSESVLESEDETIRLEITVLEETEEVAGIATRI
                     LVESEFEDGLLIEVSRNFFAQVQEGQEGAGTVCYFGEDVDECADGLEQVGDEFLCNGE
                     EPSHEGAWLAGENGNVPGLFMPADPQVGDVYMQEGADGVAEDQQEVLALGVPIEVPAG
                     DFEDTLTAEDCNPLEEGARDIKVFVRDIGLAIDEDAELIESSLIP"
     gene            complement(3937..4284)
                     /locus_tag="VI935_06175"
     CDS             complement(3937..4284)
                     /locus_tag="VI935_06175"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HLE25221.1"
                     /translation="MKKRVLIPTVVGLVIGSMLAVFVTARDTEHGRDSRLSCSVTVPD
                     PEPTNLASLAKITAGEAKVKALTGQPDGTTVIEVELENEYGCLVYGVELSNGSEVTID
                     AGNGKVLQVEPDD"
     gene            complement(4359..4628)
                     /locus_tag="VI935_06180"
     CDS             complement(4359..4628)
                     /locus_tag="VI935_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25222.1"
                     /translation="MFSSLFSHSSLVRIEYSQNIIDVYMDFKIHDPKPAHTQLPLGFA
                     IDNSTERQRQRPQEGAPQKEHYSGKVIYLRPTAGEETRQEGSRGG"
     gene            complement(4783..>5157)
                     /locus_tag="VI935_06185"
     CDS             complement(4783..>5157)
                     /locus_tag="VI935_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25223.1"
                     /translation="KYRFEGTTVGGASLKSMATLSHVIPCGPEIVSSETLPFNMARIE
                     WNAVINRLDPATEECGESTDLDIVGYQVIVGTFQVTLPASATQVTVPPEFLEPNTVYL
                     FEVLAIEASGNQTITEGSFETE"
BASE COUNT         1383 a         1247 c          956 g         1571 t
ORIGIN      
        1 ttctgtagac actataaaca ccaatgggga agcaacacag ttcgatatgc ctcatgtggt
       61 attaagcagc cccggcagat gcgtaggcct atgctctcaa ataggtttac aaccaactgg
      121 gatgaaatac taaaggtcaa agcctcattc cctattaaat ttgatagtga agctagtact
      181 taaaggccat agctaatgaa gctttggcgt tatcaaatcc ttaacttagg agatagtaat
      241 ggaagaattt aaagccatat atgatgaaaa agaagactgg ttatatctta caaagacaaa
      301 tgggagtgtc catgtcgaag atacagaaat tggtaaaggt atttatccta aaaaatgcac
      361 ctatcctatt tctactgtta cttgaaataa gatagatgca tctaaagata ggtctagagt
      421 atttgaaact ttttcctatt tcttaggaaa gtttaatttc aaacaccttg agtaatcagg
      481 caacggcttc tttcattact tctaggtgtg aatggctgca cgtactctct ggacaagctc
      541 ttctggaatc tcgtccattc catggtcttt tttagcatgt tcagcaacct tcttcataat
      601 ctcttcctct gtttcaccac ttgctaccca gtcacagtcc acacctacat ctctacatcg
      661 aatttctttt gtcattttaa acctccataa tttgtattta gaagacgtcc attgtgctac
      721 aaatggcatc tcatatcaaa tgtagcatac aaattaacaa aaaacaaaaa ttcattcatc
      781 accatccctg tttttatgta tccctaaaat gctaactatg tccatttttt tctagattcc
      841 attcaacgaa agtagaagct gcaaagacat ttggatttta ctttttcttc atctcatttt
      901 atgcacgaac cttttctatt gctattctct cacaaaaagg gatcccattt caaacatacg
      961 tataatagtt cattgccaat gaaacaagtg agaggggtaa ttggtaagga taatgcaaat
     1021 atctttagga aattgcaacg gtctcttgta tcccagtgct tgatggggtc taacacagtt
     1081 atatatgtac tcccaatgct ccagttcttt attcagttca gtcactctgt ccaagaacac
     1141 tcacttacct catagaattc ctccgtatgc gtcctctgtg ccctctctac gcagccattt
     1201 agctttggac tctttggagg taaaacgaac agatttattc cccttccctg gcattcctgc
     1261 tcaaactctc gaaaaaactc tgaaccacga tctacctgta tagctcttac cttgaatggc
     1321 atacgttcta tatctttttt tattcaccac ttatatttag ggataccttt agatcatcaa
     1381 taaatttttt aagtcttgga atgtagttag caccaaacca cccaactgaa cgtttctttc
     1441 cgtctgagaa gatttcagta ttagaactaa acttatagtc aaggggaact ggatagcgcc
     1501 cataccagat tgcactacga gtaagtcgtc taagaagatc ctcttcgtat agattaaaag
     1561 agtatccagc ttgcttagcc agctcaacta agctatggct ttgtaattct tttgggaact
     1621 tgggggtctg tttgaacttc tctttgtagt caactgaatt attgcgaact ataacagcct
     1681 taaaaatgtt ttccaccgcg taagcgatta gcataaagta agtacctaga taatagtccg
     1741 gcaaaaactg gccacttttt gttttaagaa cttcacggct attatcccag tatcgcacaa
     1801 cctcaggctc aatcagcttt gctgattcca gtagatctct ggctttcata atccaatgca
     1861 cgtcccagaa agcctttgat ctgtaatttg ttcgggaaac tcggtcccaa ttagtcatag
     1921 cttattttct ccttacaatc cttatgatca aaaatataaa gaattaataa aacatcttca
     1981 aaaatcatcg cgcgtttaca tttcctttct ccacaatcct cttattatct ctagcctctc
     2041 tctgtagctt cagaaacata tcctgatact taaccttagg gaaaacagtc attacctgag
     2101 catattcttc ttgaacaagc aaggtgttaa gcatagttcc gtcaggaaga taaacgtaag
     2161 ccagtaagcg tccgcctctt ttgtacgcta cgctgtttcc aagtttatta agtattgtgt
     2221 aagccttggg gtcttctgca tgtaacaacc tttgcaatta attcgtatcc gtttggatac
     2281 tttttcccaa gccactgctc ccctttaatc ctctgctcga ctaactgttt gctatgtcta
     2341 tctaagtgtt ctaactgtag ccttgtttgg cgttgggcat ctaaaagcat ttgttttgtc
     2401 tcttgctgtg ctttctcatg taaatcaagt agtatttgac tcatctcttc tgatgggttg
     2461 actgctttgt tgaaatctca tcctttctca tcggttgctt cttggcggaa catctagcct
     2521 tattttttat gatacttcat tgctacggct attgctaagg ttttttatgc tttctcttac
     2581 attttaatac aacgctccac ctattcaatc cttatgacaa ttacagaaac cctttaaaat
     2641 caagagtttt ctcaactcta tactttttga cacaaaatta cacaagtgct aaatttagca
     2701 cccataatcc gaaggtcggt ggttcaaatc cacccccccc gctaccaata atattaggaa
     2761 cttacaccat atcatatttg ctacaatttg aattatatta actggataat atataaaaaa
     2821 tttcccctca taaagcaaaa aaatcagtaa tctcagttgt acactagtat ggcagaatat
     2881 gtgttgtaca acgaaacaaa aggataataa ttacattaaa ataacttaat ttcagggtat
     2941 aagagaagac tcgatcaatt ctgcgtcctc gtcgattgcc aggccgatat cccgcacgaa
     3001 aacctttatg tctcttgcgc cctcttcgag cggattgcag tcctcagctg ttagtgtatc
     3061 ttcaaagtct cccgcaggaa cctctatggg cacgcccaac gctaggactt cctgttggtc
     3121 ttctgccaca ccgtccgcac cttcctgcat gtacacatcg ccaacctgcg ggtctgcagg
     3181 cataaaaaga cccggcacat ttccattttc tccggcgagc catgcacctt cgtggctcgg
     3241 ctcttcacca ttacaaagaa actcatcccc aacctgttca aggccatcag cgcattcatc
     3301 gacgtcctca ccgaagtagc aaacagtgcc cgcgccttcc tggccctcct gcacctgggc
     3361 gaagaagttc cgggagacct caatgagtaa accatcctcg aattcactct ccacaaggat
     3421 gcgtgtagct attcctgcga cctcttcggt ctcttcaaga acagtaattt ccagtcggat
     3481 tgtttcgtct tcactctcaa gcacgctctc actacctggc accacaggga agaaatcgtt
     3541 gtcgatttct agcgagaacg ggccgccggt ttctgggtcg caaacctcaa tgtccagagt
     3601 gggtactggg catcctccac ccccgccgcc gccgttgcaa ccaactcccc agaggataag
     3661 gaaaagagaa aaacataagg caaatagatg aatacatcgt tgcatggtta ttattttctt
     3721 cattattttc cctccttatg aatttattct tttactaaat agaacatagg aagattacgg
     3781 gaacctttcc tgaacattac attttggaaa ggtttataaa atgtgaagag ggtggatctg
     3841 agggtaaagc cagcggtgaa aattaaacat caactttcac tgcccgaata attagtcttc
     3901 taactcacat ttttcgctat ctattgattc tttcttttag tcgtcaggtt ctacctggag
     3961 cacttttcca ttgccggcgt ctatagtgac ttcggacccg ttgctcagtt ctactccata
     4021 gactaggcaa ccatactcgt tttcaagctc cacttcgata accgtcgtgc catcaggctg
     4081 gccggtcaga gctttgacct tagcctctcc agcagtgatt ttagccagcg aagcaaggtt
     4141 tgtaggctca ggatcgggca cagttacact gcaactcaac ctggaatcgc gcccgtgctc
     4201 cgtatcgcgg gcagtcacga acactgccaa catactgcca atgactagcc cgacaaccgt
     4261 tgggattaac actcgctttt tcattatttt cacccccctt acactgaatt tgttatgtaa
     4321 cttttatcat agtcgtggtg ttgagcagat aggtgatctc agcctcctcg gctgccttct
     4381 tgtcgtgttt cttctcccgc cgtcggccta agataaatca ctttgccact gtagtgctct
     4441 ttttgcgggg cgccctcctg cggacgttga cgctggcgtt cggtgctatt atctattgca
     4501 aaaccaagag gaagttgtgt gtgggcaggt tttggatcgt gaattttaaa atccatgtat
     4561 acatctatta tattttgcga atattcaatc cgaaccaggg agctatggga aaagagcgaa
     4621 gaaaacatct atttcaagac ctccatccgt ttttgtgcgt ttttgttccc ttttattttg
     4681 gggccacaca aaaggtggta ctccctttgc ctccttcact acaacaatgc gggatgtgga
     4741 tggagtccca gcgaccccat ccacgcagat ttagtagggg gactattccg tctcgaagga
     4801 gccctccgtg attgtctggt tcccgcttgc ctctatcgcc aacacctcga ataagtacac
     4861 cgtatttggc tcgaggaatt ccgggggaac cgtcacctgc gtggccgagg ccgggagggt
     4921 gacctgaaac gtgccgacga tgacctggta gccgacaata tctagatctg tggactctcc
     4981 acattcttca gtagcaggat ccaacctatt tataacggca ttccactcga ttcttgccat
     5041 gttaaagggc agtgtctcag acgagacaat ttcgggccca cagggaataa cgtgggagag
     5101 cgttgccatg ctcttcagac tggctccccc aaccgtcgtg ccctcgaacc tatattt
//
LOCUS       DATDRL010000178         4206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1858574, whole genome shotgun sequence.
ACCESSION   DATDRL010000178 DATDRL010000000
VERSION     DATDRL010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858574"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VI935_06190"
     CDS             complement(<1..409)
                     /locus_tag="VI935_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HLE25224.1"
                     /translation="MVFEQKHILGLEELSSDEIRLILDTAESMKEVSERDVKKVPALK
                     GVTVVNLFFEPSTRTRASFEIAEKRLSADVLNFTPTESSVVKGETLLDTARNLEAMGA
                     SVIVIRHAMSGAPWLLARELSSSIVNAGDGFHEH"
     gene            complement(415..972)
                     /gene="pyrR"
                     /locus_tag="VI935_06195"
     CDS             complement(415..972)
                     /gene="pyrR"
                     /locus_tag="VI935_06195"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HLE25225.1"
                     /translation="MKSKVIMDSETITRALSRIAHEILERNKGTKELRIIGIRRRGIH
                     LARRLADKIQELEGIKPPLGTLDITLYRDDFRRRSDWPSVKKTEIPFPVEDKNVILVD
                     DVLYTGRTTRAAIEAIMDYGRPASIQLAVLVDRGGHRELPIQADYVGIKVTTMRTQKV
                     DVLLRELDERDEVIVDQLDSTNVGG"
     gene            complement(980..1339)
                     /locus_tag="VI935_06200"
     CDS             complement(980..1339)
                     /locus_tag="VI935_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25226.1"
                     /translation="MSAKMIFWILVLVLVFYLGFEVVPIFYRGIFGIRAACKEEVEFY
                     KTYGKAFVVKRLDESLNAKGMPKDKRTYTVRVTDQAIIVTIDYYDTAEFFGGECRWCK
                     RDFEFHYNCEGALKSVY"
     gene            complement(1604..2539)
                     /gene="lepB"
                     /locus_tag="VI935_06205"
     CDS             complement(1604..2539)
                     /gene="lepB"
                     /locus_tag="VI935_06205"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HLE25227.1"
                     /translation="MLGLWSKESKIKKRAKRSVDIARSILKSNISKIDPDIGKLIKEK
                     IGETEKALGDGNLKDLTVKTEGLENVVGEHLSKFRKSRLRQNVESLLIAVALALFIRA
                     FIVQPFKIPSGSMIPTLLVGDHLLVSKFIYGTKIPFTDKIVLPVNNINRGDVIVFAYP
                     NYEDDPSKDGIDYIKRVVGLPGDSIDIEGRNLYVNGEEVPLKYIGEFHDERIGVGYDE
                     YEEDLFGNKHKVIYLKGKGSTERGGYIPVTKVPEGYVFVMGDNRDNSQDSRFWGFVPV
                     KDIAGNAFLIHWSWDFGSEGILDKVRWHRVLSLIE"
     gene            complement(2860..>4206)
                     /locus_tag="VI935_06210"
     CDS             complement(2860..>4206)
                     /locus_tag="VI935_06210"
                     /inference="COORDINATES: protein motif:HMM:NF040246.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HLE25228.1"
                     /translation="RECYEALGAVHAAWKPVPGRFKDYIALPKGNGYQTLHTTVIGPF
                     GERMEVQIRTQRMHEIAEYGIAAHWKYKEGRIEAVESDKIYASLRQLLEWRDIKDPTE
                     FLEAVKGELIPNMVYVFTPKGDLKELPIGATPVDFAYSIHTEVGNHCTRARVNGKLVP
                     LDYKLRSGDTIEIATLPDKKPSRDWLRFVITSRAKTKIRSWLRTEEREQSKHVGKSIC
                     ERKFKQHSLDYQKMLKSGELNTPLSELGFKEIDEFYLALGYGKVSVTELLKRILPKER
                     LEGLPEKESMLERIIQTLTRSTKSGVLVKGFDDVMIRFGNCCTPLPGENIVGYITRGR
                     GITIHKHNCSQLLDVDPARKIDVAWDRGFKEPRPARIEVICADRPGILSSITRSIASS
                     DINISKAEIHSTDDERAIGTFEIAVSDLNQLEGVMKSIKRLKGVISVERILGAEGI"
BASE COUNT         1139 a         1005 c          683 g         1379 t
ORIGIN      
        1 gatgctcatg aaaaccatca cctgcgttaa cgattgaaga gctaagttct cgggcgagaa
       61 gccatggagc accagacata gcatgcctga tcactatcac acttgctccc atagcttcaa
      121 gatttctagc agtatcaagt aaagtttcac cttttacaac actactctca gttggcgtga
      181 aatttaatac atcagcgcta agcctttttt ctgctatctc aaatgacgcc cttgttcttg
      241 tgctaggctc gaagaataga ttaacgactg taacaccctt gagtgcagga acctttttta
      301 catccctctc tgatacctcc ttcattgact cggcggtatc aagaattaac cttatttcat
      361 ccgatgataa ctcttcaagt ccaagtatat gcttttgttc aaaaaccatg tccgctatcc
      421 acctacgttt gtcgagtcaa gttgatccac tattacctca tccctttcat ctagctctct
      481 gagaagtaca tctacctttt gagttctcat agtcgtaacc ttaattccca catagtctgc
      541 ctgaatcgga agctccctat gacctcccct gtcaacaaga acggcgagtt gtatcgaggc
      601 gggtctccca taatccatta tggcctcaat agcggccctt gtagttcttc ccgtataaag
      661 aacgtcgtcc acaagaatca cgtttttatc ctccaccgga aatggaattt cagtcttttt
      721 tacactgggc cagtcgcttc ttcttctaaa atcatccctg taaagcgtta tatctagtgt
      781 tccaagaggc ggctttatgc cctctagttc ttgaatttta tctgcaagcc ttcttgctag
      841 gtgaattccc cttcttctta tgcctataat ccttagctct ttcgtgccct tatttctttc
      901 tagaatctcg tgtgcaattc gcgaaagggc gcgggttata gtttccgaat ccataattac
      961 tttgcttttc ataacgaaac taatatacgc tctttaacgc gccctcacag ttataatgga
     1021 attcgaaatc ccttttgcac cacctacact ctcccccgaa gaactctgct gtatcatagt
     1081 agtctatcgt tactataata gcttggtctg ttaccctaac cgtgtatgtt cttttatctt
     1141 tgggcatccc ctttgcattt agtgattcat caagccgttt aacaacaaac gctttcccgt
     1201 atgttttata aaactctacc tcctccttac aagccgccct tataccgaat attcctctat
     1261 aaaatatcgg aactacctca aaccccagat agaaaacaag caccaaaact agaatccaaa
     1321 atatcatctt tgcactcata tgataaccct catgtttaaa tcaaattaat aaacagtttt
     1381 atcaatatat tacttccata ttctcattaa ataaagctgc taacaattca aaaagtgtat
     1441 gtataaatct caaatgcgtt gtttaattac tgtattgtta ttatgatgct agttcgccaa
     1501 tcttaagaga tcagtttgtt caactcattt tagctctgtt taggtcgaca attttatagc
     1561 ggtctcattt tgagttagat atatataata gcacctgccg atactattca attaaagaaa
     1621 gcactctatg ccacctgact ttatctagta taccttcgct accaaagtcc catgaccaat
     1681 ggatgagaaa agcattgcca gctatatctt ttactggcac gaatccccag aatcgactgt
     1741 cttgactatt gtcccgatta tcacccatga caaatacata accctctgga acctttgtca
     1801 cagggatgta cccgcccctt tctgtagatc cctttccctt tagatatata accttatgtt
     1861 tatttccgaa gagatcttct tcatattcat cgtaaccaac accaattctt tcatcatgaa
     1921 actcccctat gtatttaaga ggaacctcct ctccatttac atacaaattt ctaccttcta
     1981 tatctatgct atcgcctgga agacctacta ccctctttat ataatctatt ccatcctttg
     2041 aagggtcatc ctcgtaattg ggatatgcga atactatcac gtcaccacga tttatattat
     2101 taacaggcag aacaattttg tccgtaaatg gtatcttagt cccataaata aacttactta
     2161 caagtaggtg atcacctaca agcagtgtgg gaatcataga tccagatggg attttaaaag
     2221 gctgtacaat aaatgctctg ataaaaagtg caagtgctac tgcgataagt agggattcga
     2281 cattttgtct taaccttgat ttcctaaact tagataggtg ttctccaact acattttcaa
     2341 gaccttcggt tttcacagta aggtctttca agttaccgtc tcctaaagcc ttttcagttt
     2401 caccaatttt ttcttttatt aatttcccta tatcggggtc gatttttgaa atattactct
     2461 taagaataga tcgagcaata tcaacagatc tttttgccct ctttttgatt ttagattcct
     2521 ttgaccaaag tcctaacatc ttgagggttt gagtataaat gggaaggtgg attctttcaa
     2581 tatgtataga aacctgtgta tagaaacctg gaaattacta atcctagaat tcacaatttg
     2641 gaattaatgt atctatatac aaatctattt tgctaaatta gttgaagatt atatcctgtc
     2701 tgcagcaatg aacctgatca ttacctaagg agtagtgagg aatctcaaag ggattgcttc
     2761 gtcacggagt ttatcctgag cgaagccgaa gggttcctcg agcaacttgt ctgtttaaac
     2821 gctgatacct tgcagctccc tgtatggttc ttaattaatc tatattccct cagctcctaa
     2881 tattctttct acagatataa ccccttttaa cctttttatt gacttcatta cgccttctaa
     2941 ttggttaaga tccgagaccg ctatctcaaa tgtccctata gctctctcgt catcagttga
     3001 atggatttct gctttagata tgtttatatc agacgacgca atagagcgcg tgatgcttga
     3061 taatattcca ggacggtctg cacaaataac ctcaatcctt gcaggcctag gttccttaaa
     3121 gcctctgtcc caggcaacat ctatctttct cgcaggatca acgtcaagaa gctgggaaca
     3181 gttgtgttta tgtattgtaa ttccgcggcc tcttgttatg tatccaacaa tgttttctcc
     3241 tggaagtgga gtacagcagt ttccaaacct aatcattaca tcatcgaaac ctttaactag
     3301 aactccgctt ttcgtcgagc gtgtgagggt ctgtattatc ctctcaagca tagattcctt
     3361 ctcaggcaaa ccctctaacc tttccttagg gagaatacgt ttgagaagct cggttacaga
     3421 aaccttgcca tagccaagtg ccaaataaaa ctcgtcaatt tccttaaatc caagctcact
     3481 aagtggtgta ttaagctcac cgctctttaa catcttttga taatcaagac tatgctgttt
     3541 gaatttcctc tcacatatag atttccctac gtgcttcgat tgctcccttt cttccgttct
     3601 aagccatgaa cgaatctttg tcttagcacg agaggtaatt acaaacctaa gccagtcgcg
     3661 acttggtttc ttgtcaggca aggttgcaat ttctattgtg tcaccacttc ttagtttata
     3721 atcaagcgga actagcttcc catttaccct tgcccttgtg cagtggttcc caacctctgt
     3781 atgaatcgaa tatgcaaaat caacaggtgt tgctccaatg ggaagctctt ttagatcccc
     3841 ctttggggta aacacataaa ccatattagg aattaattca ccttttaccg cttcaagaaa
     3901 ctctgtcgga tcttttatat ccctccattc aagaagctgt cttaagctgg catagatttt
     3961 atcgctctct acggcttcga tccttccctc tttgtatttc cagtgagctg caataccgta
     4021 ttcggcaatc tcgtgcatcc tttgggtcct gatctgaacc tccatccttt caccaaatgg
     4081 accaataacc gttgtgtgaa gagtctggta cccgtttccc tttggaagag caatgtagtc
     4141 cttgaatctt cctggaactg gtttccaagc tgcatgaact gcaccaagag cctcatagca
     4201 ttctct
//
LOCUS       DATDRL010000179         5010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2113594, whole genome shotgun sequence.
ACCESSION   DATDRL010000179 DATDRL010000000
VERSION     DATDRL010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113594"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="VI935_06215"
     CDS             <1..1216
                     /locus_tag="VI935_06215"
                     /inference="COORDINATES: protein motif:HMM:NF039926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HLE25229.1"
                     /translation="LLCPGCGDVVESFSTLRNLDSHYHCDLCQKDFEATLDDYIEISF
                     TISPSIRKIVFHNPETLSIEDYFFKYVFSEGAMFPDGTQFVDLVKKSVIILTYLDAKE
                     KKKFELEIPPGFLVGCDRLNHGEFFFNVNGEYKPEIQHLSFKLVDGKYEPEKGELFPG
                     KFVFDFENHTNKRGALLLIYKPHDFSPPGPLIYKPFLSGKRLLTTQTFRDLFRTEIVQ
                     GNEGIGIKDITILFTDLKGSTALYDRIGDLKAFSLVQQHFESLGKVVNNHSGAILKTI
                     GDAVMATFLNPLDAVKAALSMYEEIERFNQEHGERELILKIGIHKGAAIAVTLNDRLD
                     YFGQMVNISARVQKLSDADEIHITDEVYTYPGVRELLNGFEINLRKAKLKGIKEEMRV
                     YRINHNQRKIIE"
     gene            1492..3285
                     /locus_tag="VI935_06220"
     CDS             1492..3285
                     /locus_tag="VI935_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HLE25230.1"
                     /translation="MILKRSALLLMLILLPTAVFAAKRYVVKKGDNLGNLAKRFRVSI
                     QSIKKTNGLNTDRLNVGKTLIIPDAINNQTNVIASKDSEKNENELEVNKTNLQSFSLR
                     YIVKKEDTLSGIANRFGISVEKISYVNGLTENGLKTGMILTIPDSRNKDLGNMIFSPT
                     AQVGVEPKLVSSDGHREYIVKDGDSLTSIAEGCGVSVSDLKNANGLSSEEVRIGTKLI
                     IPDSQIPMRVVRVSPNVNETNNYIVKKDDALSTIASKYNVSVKNLMSANNLKSNEVRI
                     GTKLIIPDSQIPMRVVRVSPNENEINKYVVKKGDTLSKIASRYRVSVKDLKNVNGMKS
                     DKVKFGMSLIIPNSTLSRNVEPKTPVISDNNKYIVQKGDNISSISRRYGVSQAELKRA
                     NNIKGDTIKSGQLLRVPSKINGKNELVVEDIKVEDNDTHINSLPEIETAKAIQSNDSS
                     TYENGLTRDVIISIAKRFLGAPYKFGGDSLIRGLDCSAFVNKVFTFFNVDLPRTAREM
                     YKIGRNVTKGELEAGDLVFFKTYASYPSHVGIYIGDSEFIHASSAAKRVRIDNIDQSY
                     YRKRYIGAKRVVSTGIFYEELSKDYKGFEKQ"
     gene            3579..4475
                     /gene="xerC"
                     /locus_tag="VI935_06225"
     CDS             3579..4475
                     /gene="xerC"
                     /locus_tag="VI935_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HLE25231.1"
                     /translation="MFVHFNRFREFLKSERNYSEHTVRAYMTDLLQFSCFLREKVFNS
                     ACDEFDFSSVDEVAIRAFLGWLHRRKNLKSSISRKLASIRTFFEFLLREGVVNRNSAK
                     LVPTPKREKKLPAFLTVDEVFTLLDTEYANSAIGYRDKAILELLYSSGLRVSELVGIS
                     LSDINLTNSLVKVLGKGGKERIIPFGSKALNAINLYIPKRSELKPRGDFLFVNSRGGR
                     LTSRSVGRIVKRYASMSKIPKSVSPHVLRHAFATHLLGSGADLRAIQEMLGHSSLSTT
                     QRYTHTSIEKIMEIYDKTHPRA"
     gene            4482..>5010
                     /gene="hslV"
                     /locus_tag="VI935_06230"
     CDS             4482..>5010
                     /gene="hslV"
                     /locus_tag="VI935_06230"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HLE25232.1"
                     /translation="MNKFHGTTIVCVRKDGRVAMGGDGQVTLGQTAIKHTAKKVRKIY
                     NDRVMVGFSGATADALTLFDRFESKLEEYRGNLRRATVELARDWRTDRILRRLEALLA
                     IADKENMFLISGSGDVIEPDDDVLAIGSGGPYAQAAAKALLRHSKIDARGIVEEALRI
                     AAEICIYTNDKIIVEV"
BASE COUNT         1648 a          770 c         1072 g         1520 t
ORIGIN      
        1 gcttttatgc cctggttgtg gagatgtagt tgaaagcttt agcacgctga gaaatctaga
       61 ttctcattac cattgtgact tatgtcagaa ggattttgaa gctacgctcg atgattacat
      121 tgagatcagc tttactatat ctccatcgat caggaagata gtttttcata atcccgaaac
      181 tctttcgata gaagattatt ttttcaaata cgtatttagc gaaggcgcca tgtttccaga
      241 cggaacacaa tttgttgatt tggtgaagaa gtcggtaata atcctcactt atctcgatgc
      301 gaaagaaaag aaaaaatttg aactcgaaat cccaccggga tttttggtgg gatgtgatcg
      361 gctaaaccat ggggagtttt tttttaatgt gaatggggaa tataaacctg agattcagca
      421 tttatctttc aaattggttg atgggaaata tgagcctgag aagggtgaac ttttccctgg
      481 aaaatttgtt ttcgatttcg aaaatcatac gaacaaaaga ggagcactat tattaatcta
      541 caaaccccat gatttttcac cacctggacc acttatctat aagccctttc tttctggaaa
      601 gaggcttctc actacacaaa cctttaggga tcttttccgc accgaaatcg ttcagggaaa
      661 cgagggaatc ggaatcaaag atatcactat tctttttaca gacttaaagg gatcaaccgc
      721 gctttatgat cgtatagggg acctaaaagc cttttcatta gttcaacaac attttgaaag
      781 cttagggaag gttgtaaaca atcattctgg agctattttg aaaacaatcg gagatgccgt
      841 tatggctact tttcttaatc cgttagatgc agtaaaagca gctctatcta tgtacgagga
      901 aatagaacgg tttaatcaag aacatggtga aagggaatta attttaaaga tcgggatcca
      961 taaaggggca gctattgcag taacactaaa tgatcgtctg gattatttcg ggcagatggt
     1021 aaatatatcc gcgcgcgttc agaaactatc cgatgcagat gagattcata ttacagatga
     1081 agtgtatacc tatcccgggg ttcgggagct gttaaatggg tttgaaatta atcttagaaa
     1141 agcaaaacta aaaggtatca aagaggaaat gcgggtttac agaattaatc acaaccagag
     1201 aaaaataatt gaatgattta atcaaaattt ggtgatgttc tttatattgt tattaaaaga
     1261 ttacatagat acctattaac acacatttat attttattat tcatactgaa aaaatttttt
     1321 tctatttgct ctttacatat taattctgat tagtatataa ttatgtgctt ttttatctgc
     1381 tctttgaaat tagttgcttt tattctttac aagagaagta cttattagtt gtttagttat
     1441 aatctagggg ggtggttagt ttctacgatt ggcttgtcta ggaggtgtaa tatgatacta
     1501 aagcgttctg cacttttgct aatgttgatc cttctcccca ctgctgtttt tgctgctaag
     1561 agatacgttg ttaaaaaggg agacaattta ggcaatttgg caaagaggtt tcgagtttct
     1621 attcaatcta taaagaaaac aaacgggtta aacactgata gattaaatgt tgggaaaacc
     1681 ctcatcatac ctgacgctat aaacaatcag accaatgtaa tcgcttctaa agattcagag
     1741 aaaaatgaaa atgaacttga agtaaataaa accaacttac aatcgttttc actaaggtac
     1801 atagttaaaa aggaggatac gcttagcgga atagctaatc gatttggtat ttctgttgaa
     1861 aaaattagtt atgtgaatgg gcttaccgag aacggtttaa aaaccggtat gatactcacc
     1921 attcctgatt ctcggaataa ggatctaggt aatatgattt tctcacctac agctcaagta
     1981 ggtgtagaac ctaaattggt ttcgtctgat gggcatcgcg aatatattgt taaagatgga
     2041 gactcgttaa caagtattgc tgaaggttgc ggtgtctccg tgagcgattt aaagaacgct
     2101 aacgggttat cgagtgaaga agtaaggatt gggacgaagc tgatcatacc tgattctcaa
     2161 attccgatga gggttgtcag agtttctcca aatgtaaatg agactaataa ctatatagtt
     2221 aaaaaagatg acgctttaag cacaattgca agcaagtata acgtttctgt aaagaattta
     2281 atgagcgcaa ataatttaaa gagcaatgaa gtaaggattg ggacgaagct gatcatacct
     2341 gattctcaaa ttccgatgag ggttgtcaga gtttctccaa atgaaaatga gatcaataaa
     2401 tatgtggtca aaaaaggtga caccttaagt aaaattgcga gtaggtaccg cgtttcagta
     2461 aaggatttaa aaaacgtaaa tggtatgaag agtgataaag tgaaatttgg tatgagtctc
     2521 attattccga actccacttt atcaagaaat gttgaaccta aaactccggt gataagcgat
     2581 aataacaagt atatagttca aaaaggagat aacattagtt cgatatccag gcgatacggt
     2641 gtttcacaag cggaattaaa gagagcaaat aacataaaag gggatactat aaaaagtggt
     2701 cagcttctta gagttccctc taagattaat ggaaagaatg aattagttgt tgaggatatc
     2761 aaagtagaag acaatgatac gcatattaat tcattaccag aaatcgagac tgcaaaagca
     2821 atacaaagta acgattcaag cacatatgaa aatggtttaa ctagggacgt tattataagt
     2881 atagcaaaga ggtttcttgg ggctccctat aagtttggcg gcgattcact tataaggggt
     2941 cttgattgtt cggcatttgt taataaggtc tttactttct tcaatgttga tctaccaaga
     3001 acagcgaggg aaatgtacaa gattggacgg aatgttacta agggtgaact agaggctggc
     3061 gaccttgtgt tttttaagac ctatgcaagc tatccatcac atgttggaat ttatataggt
     3121 gacagcgagt ttatccatgc ttcatctgct gccaaaaggg ttagaattga taacattgat
     3181 caatcttatt atagaaagag atacatagga gctaagagag ttgttagcac aggcatattt
     3241 tatgaagagc tgtctaaaga ttacaaagga ttcgagaaac agtaagtagg atatcctctc
     3301 ttttatttgc gcctttcctg gtggggttat aattttacaa agtgtttata gttatgctat
     3361 aaaaatgcct gtactaaaat cattctgtgc aagttgatta gttaataaat aagtaaaaga
     3421 taaccttttg tgttagtttt aagtaagatg tatttctaat atacggagta gatacccctt
     3481 ctaaataatc ctcagttaaa taaaaccctc attcttacga tcgttaaaca gatttgaaat
     3541 gctaaaaagt ggtatgtata ttattttaaa gaaaatttat gtttgttcat tttaataggt
     3601 tcagagaatt tcttaagtct gaaaggaatt attcggaaca tacagttaga gcctatatga
     3661 ccgatctttt gcaatttagt tgcttcctga gagagaaggt ttttaattct gcttgtgatg
     3721 aatttgattt ttcttctgta gatgaagttg ctataagggc atttcttggt tggctccatc
     3781 ggaggaagaa tttaaaatcg tcaatatcaa ggaaactcgc atcgattaga accttttttg
     3841 agtttctttt gagggaaggt gtagttaaca ggaattctgc aaagcttgtt cctactccaa
     3901 agcgagaaaa gaaactccct gctttcttaa ctgtagatga ggtatttaca ctacttgata
     3961 cagagtatgc taatagtgcc ataggatata gggataaagc tatactagag cttctttatt
     4021 caagtggtct tagagtaagt gaacttgtag ggattagctt aagtgacata aatctcacaa
     4081 actctcttgt aaaggtttta ggaaaaggag gaaaagaaag aattattccg ttcggatcaa
     4141 aagctcttaa cgctattaac ctatatattc ctaaaagaag cgaacttaaa ccaagaggcg
     4201 acttcctttt tgtaaactct cgtggtggta gactcacttc aagaagtgta ggaagaattg
     4261 tcaaaagata tgcaagtatg tcaaagattc caaagagtgt aagcccacat gtattgaggc
     4321 atgcatttgc gactcacctg ttaggtagcg gtgctgatct acgcgcaatt caggagatgc
     4381 ttggacatag tagcctttct acgacacagc gttacaccca cacttcaatt gagaagatta
     4441 tggagattta tgataaaacc catccgagag catagacaga tatgaacaag tttcatggta
     4501 caacaatagt ttgtgtaaga aaagatggtc gtgttgcaat gggcggcgac ggtcaggtga
     4561 ctttaggtca aactgctata aaacacacgg ccaagaaggt aagaaagatt tataacgata
     4621 gggttatggt cggtttttcg ggagctacag ccgatgcgct tacccttttt gataggtttg
     4681 aatcaaagct tgaggagtat agaggtaatc tgagaagagc aaccgtggag cttgcaaggg
     4741 attggcggac tgacagaata ttgagaaggc tagaggcact acttgcaatt gcggataagg
     4801 agaatatgtt tctaatttct ggaagtggtg atgtcattga acctgacgat gatgtactag
     4861 caatcgggtc aggcggaccc tatgcacagg ctgcagcaaa agcattactt cgacattcta
     4921 aaatagatgc aaggggaatt gttgaggagg ctcttagaat cgcggcggaa atatgtattt
     4981 atacgaatga taaaatcata gtagaggtgc
//
LOCUS       DATDRL010000180         3100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2435640, whole genome shotgun sequence.
ACCESSION   DATDRL010000180 DATDRL010000000
VERSION     DATDRL010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2435640"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VI935_06235"
     CDS             complement(<1..624)
                     /locus_tag="VI935_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25233.1"
                     /translation="MKSLRIALFTYSTKPRGGVVHTLSLAEELARLKHRVHIYALGSK
                     EVSAVMSKPAPSLVEGTSDLPNIRVNRTKKRGSPPDPALSKVEGLLVDEKSEFFRPVH
                     VPYTIISCSTLPEENIDEKVKRYINTYTDYLSSVREHYDIYHAEDCISANMLCELRNR
                     GLIKFFVRTVHHMDDFTSKSLIDCQLKSILEPDYVIVVSRFWEKELRS"
     gene            complement(705..1712)
                     /locus_tag="VI935_06240"
     CDS             complement(705..1712)
                     /locus_tag="VI935_06240"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HLE25234.1"
                     /translation="MSTKQKELSNFLTAEAIGYKALKALSGSSRTAKVHSIFDRAFYI
                     RIDRNTLISVIKNKDYISPTSISVKESKDKSFKSIGIKEGMKIVIDNNSLLFGDNALT
                     IKLRKSSNWFPPLIPEQDDLTGLTGIGLNLRILRDLIYTSPTREGLVPLLENVELYGP
                     LNIFVRPQEPGVSERARPNIETMMWGLYRGDLDMVLKNVESILGLGPGLTPSCDDFLA
                     GLIHSLTIGGKALLKKSKGELGFYRKVSAEICRLAKIKTTIYSQILLNQARFGEGPKA
                     VVDLVHSLLTKQPPHVAEVSKVVINMGETSGADIAIGIFYGIRFLLSRMERIEDLYQF
                     E"
     gene            complement(1855..2130)
                     /locus_tag="VI935_06245"
     CDS             complement(1855..2130)
                     /locus_tag="VI935_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25235.1"
                     /translation="MTGKKSFGEWLRENAEKYLLEAASDEMMARYPEFCAKPYRDKGF
                     IPFFWRNIFVAIYLRIPWSVRRKMILLSSYPTGERPHWRRHSHEQDS"
     gene            complement(2147..>3100)
                     /locus_tag="VI935_06250"
     CDS             complement(2147..>3100)
                     /locus_tag="VI935_06250"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLE25236.1"
                     /translation="ILDQIKKVVVMAATPLGIEMTGNLAHRGLETHLVDEGGWIMSKV
                     ADPDIVEPVQKSLEEFGAKMHFGTKVLEFKGSNGKAKSVLTTNGEIECDVVIVATNKV
                     PNNRLSRQAGLEIGATGGLLVDDHMRTSVKDVYAAGDCIEVIHGVTDLPIQGLSGSHA
                     YSQGRVAGANAAGHDRSYDPVFIPWGMVGGKVQIGGVSLGETLHKALGIPHMVAAAQG
                     ISRARYYPGVSRIRVKLIADPKTLRVMGAQMAGGEGIKERADFLAFAIKRGATLHDFA
                     WMENVYSPPIGALMEPIALAAQAGLAELTKRGYQPPKFAGA"
BASE COUNT          849 a          749 c          576 g          926 t
ORIGIN      
        1 agagcggagt tctttttccc agaacctact gacaacgatt acataatcag gctctagaat
       61 gctttttaac tggcaatcaa ttagcgactt agatgtgaaa tcatccatat gatgaaccgt
      121 tcttacaaag aatttgatta aacctcgatt gcgtaactca catagcatgt ttgcagagat
      181 acaatcttcg gcgtgataga tgtcgtaatg ttctcttact gaagaaaggt aatcagtgta
      241 cgtattgata tatctcttta ccttttcatc tatgttctct tcggggaggg tagagcagga
      301 aataatagta taaggaacat gcacgggtct aaaaaattct gacttttcgt ccaccagcaa
      361 cccttcgact ttgctcaggg cggggtctgg tggactaccc cgtttctttg ttctatttac
      421 ccgtatattg ggtaggtcac tagtcccctc gaccaagctc ggggcaggct tgctcatgac
      481 cgcgcttact tctttcgaac ccagagcgta gatatggact ctatgtttaa gacgggctaa
      541 ttcttcagct aggcttaatg tatgtaccac tccgcccctc ggttttgtcg agtaagtaaa
      601 aagcgctatc ctaagcgatt tcatctaatt cccaatttgt aggcacagac ttgtctgtgt
      661 tatacctttg gcacgaataa attcgtgcct acaaaagtta ttttttattc gaattggtat
      721 aaatcctcta tcctctccat tcgtgaaagt aaaaaacgaa tgccataaaa aataccaatc
      781 gcaatgtcag ctccagaggt ctcacccata tttatcacca cctttgaaac ctcagcaaca
      841 tgaggtggtt gttttgttag caggctatga accaagtcaa caacagcttt gggtccctca
      901 ccaaagcggg cttgatttag aaggatctga ctatatattg tcgtctttat ttttgctaat
      961 cggcagattt cagccgatac ctttcgatag aagcctagct cacctttact ttttttgaga
     1021 agtgcctttc cacctatagt taaactatgt atcaaaccag ctaaaaagtc atcgcaggaa
     1081 ggcgtgagcc cggggcctaa tccgagtatt gactcaacat tctttaacac catatccaaa
     1141 tcacccctat acagccccca catcatggtc tcaatatttg gccttgctct ctctgaaaca
     1201 ccaggttctt gtggtcttac aaagatgtta agtggtccgt ataactctac attctctaaa
     1261 agtggcacaa gcccttccct agtcgggctt gtgtaaatta agtctctaag gattctaaga
     1321 tttagaccaa tgcctgttaa acctgtaaga tcatcctgct caggaattag aggaggaaac
     1381 caattagagg attttctgag tttaatcgta agcgcattat ctccaaagag caggctatta
     1441 ttatcaatca ctatcttcat tccttcttta ataccaattg acttaaagct cttatcctta
     1501 gattctttta ccgagatgga tgtaggactg atatagtctt tgtttttgat tacactgatt
     1561 agagtgtttc tatcaatccg aatataaaat gcacgatcaa aaatactatg taccttagct
     1621 gtcctgctag aaccgctcag tgccttcagt gctttatagc caatagcttc tgctgttaaa
     1681 aagttagaaa gttctttctg ctttgtagac atttaagtat ttgtttatta tggtctttga
     1741 ttaaatagat aacaatctga ttagcacaga caattgtagg atcgttccta ctcgttgccg
     1801 tccaccagcg agactggtgg actacccatt tcataattcc gtctattggg taggtcacga
     1861 gtcttgctcg tgactgtgcc tcctccaatg tggacgttct cccgttggat aagaagaaag
     1921 aagaatcatc ttcctacgaa cactccaggg aatccttaaa taaatcgcaa caaagatatt
     1981 tcgccagaaa aaaggaataa atcccttatc acgataaggc ttcgcacaga attcgggata
     2041 acgggccatc atttcgtcag aagcagcttc aagcagatat ttctcagcgt tttctcttaa
     2101 ccactcgccg aaggattttt ttccagtcat ttaaacttcc ttttttctaa gcaccagcaa
     2161 attttggagg ctgatatccc ctcttcgtca actcggccaa tcctgcctgg gcggctaaag
     2221 caattggctc cattagcgct ccaatcggag gagaataaac attttccatc catgcaaagt
     2281 cgtgtagcgt ggctccacgc tttattgcaa acgcgagaaa gtccgccctt tctttaatcc
     2341 cttcaccccc tgccatctga gcacccatta ctcgaagcgt tttcgggtcg gctatgagct
     2401 tcacacgaat cctgcttact ccggggtagt atcttgccct ggagatgccc tgagctgcag
     2461 ctaccatatg aggaattccc aaagccttat gcaatgtctc gcctagtgaa acccctccaa
     2521 tctgaacctt tccgccaacc attccccaag gaataaatac tgggtcatag gagcgatcat
     2581 gaccggctgc attagcacct gcaacccttc cctgagaata ggcatgactg ccagataaac
     2641 cttgaattgg cagatccgta acaccatgaa taacctcaat acagtcccca gcagcatata
     2701 catctttgac agatgtacgc atatgatcat ccaccaaaag accgccagtg gctccaattt
     2761 caagtcccgc ttggcgtgat agtctgttat taggaacctt atttgtagca actataacaa
     2821 catcgcattc tatttctcca ttagttgtga ggacagattt cgcttttcca tttgaaccct
     2881 taaactctag cacctttgtt ccaaaatgca tctttgcacc aaattcctct agtgatttct
     2941 gaaccggctc tactatatct ggatcagcaa ctttagacat tatccaaccc ccctcatcca
     3001 ctagatgagt ttcaagtcct cgatgcgcaa gatttccagt catctcaata ccaagtggtg
     3061 tcgcggccat gacgacaact ttctttatct gatcaagaat
//
LOCUS       DATDRL010000181         5744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1596192, whole genome shotgun sequence.
ACCESSION   DATDRL010000181 DATDRL010000000
VERSION     DATDRL010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596192"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VI935_06255"
     CDS             complement(<1..184)
                     /locus_tag="VI935_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HLE25237.1"
                     /translation="MGVNTNPVLGGDGFITTKLSSFANWARKNSLWPMPFGTACCAIE
                     MMGAFASRFDLSRFGAE"
     gene            complement(175..531)
                     /gene="ndhC"
                     /locus_tag="VI935_06260"
     CDS             complement(175..531)
                     /gene="ndhC"
                     /locus_tag="VI935_06260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HLE25238.1"
                     /translation="MLEDYLPILIILIFSVLLAGVLLGVSNLLGPKRYSKKKLTPYEC
                     GVPPVGDARERFSVKYYLVAALFILFDVEAVFLFAWAVVFKRLGVLAFVEIIAFFIVI
                     LGGYLYVLKKGALEWE"
     gene            complement(925..2358)
                     /locus_tag="VI935_06265"
     CDS             complement(925..2358)
                     /locus_tag="VI935_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLE25239.1"
                     /translation="MNERDPLKDIELLILSRYCIIFLDTVEEERAETLLKHLADHLRL
                     PFFSWTRTKGLGREEVKGHVYVSADPSIALTHIEHSQLPAIYHFQGLGDYLEDKLIVS
                     KLKDAAINFSKRSGAIIITGDDNIPDILRPISAKIELIAPRIEEYSELLNNIIRDLNV
                     RMNVKVEISPEDTNRLLNNIKGLTLMEAEKIFTMLIVEDGRLSPEDIRQVVEAKKGIV
                     EREGLLEYYPVEESMADIADVLGLKSWLAKRRNIVTDPKRASDFGLSFPKGVLLLVVP
                     GCGKSLCAKAVAMEWGLPLLKLDPSNLYNKYIGESERNFKKAMKTAERLAPIILWIDE
                     IEKAFSSSDTTEDNGVSLRIFGSFLSWLQDRKGDVFIVATANDVMKLPPEFLRKGRFD
                     EIFFIDLPNPEARKSIFDIHLKKRGKQPHLFNVTLLVETTEGFSGSEIEQVIVSGLYS
                     AFSAKTELSTQMLLEEISLTHPLSVTM"
     gene            complement(2899..3849)
                     /locus_tag="VI935_06270"
     CDS             complement(2899..3849)
                     /locus_tag="VI935_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HLE25240.1"
                     /translation="MIGTRGISIKMPGRSYSGQFTPLTEEETEISSHIRKHVEVLAGE
                     IGERNLREYNNLEAAISYIEKTLRELNYGVSFQDFVVEGKIAKNIEVEILGKSQPGEI
                     IILGAHYDSVINCPGANDNATGVAAILEFARLLKGGNLQRTVRLVAFMNEEPPFFQTE
                     NMGSLVYARRANKRREKIVAMFSIETIGYYSDAKGSQRYPFPLSFFYPDTGNFIGFVG
                     NTSSRSLVHQSIAFFRKNTYFPSEGVAAPGWIPGVGWSDQWSFWKEGYSAIMITDTAL
                     FRYPYYHTQWDTPDKIDYNRMARVVAGLKQVVIELASADK"
     gene            complement(4112..4522)
                     /locus_tag="VI935_06275"
     CDS             complement(4112..4522)
                     /locus_tag="VI935_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25241.1"
                     /translation="MPIKITNIVNLQQIEAVVSGPDGANRLFVITGIIPTNLSTNTTI
                     KETFTFLIGPTVTRRQFVRAIATASLNRILLFSGAAGPSIINSNWSVESVDADWDDES
                     GQVEVRVEVLTSSGGTGSTGVTSIGFQATILAEI"
     gene            4818..5549
                     /locus_tag="VI935_06280"
     CDS             4818..5549
                     /locus_tag="VI935_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HLE25242.1"
                     /translation="MEDKIREAKEILDRASNITVLTGAGISAESGIPTFRGEEGLWKK
                     FRSEELASQEAFQRNPKLVWEWYDWRRGIAKDAKPNLGHYALVNLENEKSNFTLVTQN
                     IDGLHQLAGSRNIIEMHGNQWQIRCTSCGIIEQNHDVPLNELPPKCKNCGALGRPNVV
                     WFGEIIPMAIIDNILIAIERCEVMLIVGTSGVVEPAASMGLIAKHTGKTVIEVNLETT
                     PNSSLYDISILGKSGEILPILCKSN"
     gene            complement(5546..>5744)
                     /locus_tag="VI935_06285"
     CDS             complement(5546..>5744)
                     /locus_tag="VI935_06285"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006115913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HLE25243.1"
                     /translation="LIPFKMLSFDDGVNVTLGLPIIRTSPDHGTAYDIAWRGKANPSS
                     MIAAIKVAVRLVQNKRKRNTG"
BASE COUNT         1832 a         1218 c         1073 g         1621 t
ORIGIN      
        1 cctctgcacc aaaccttgaa agatcaaatc ttgatgcgaa cgcccccatc atctcgattg
       61 cacagcatgc agtcccaaat ggcattggcc agaggctgtt tttcctcgcc cagtttgcaa
      121 atgagcttag cttagtagtg ataaaaccat ctccgccgag tacagggtta gtatttactc
      181 ccattcaaga gctccctttt ttaatacata caagtagccc cctaagataa cgataaaaaa
      241 ggctataatt tcaacaaatg caagaacccc aagcctttta aatacaacag cccacgcaaa
      301 aagaaaaaca gcttcaacat caaaaagtat gaaaagtgcc gcgactaaat aatacttaac
      361 cgaaaacctt tcccgtgcat ctcctactgg aggaacgccg cattcataag gagtaagctt
      421 tttctttgaa tacctctttg gaccaagcag attagaaacc ccgagaagga ctccggcaag
      481 cagcacagag aaaatcaata taattaatat tgggagataa tcctcaagca ttttattatt
      541 aggttaagtt ataagtggag attgtcaagc aaagtctaac taggggatat tatttggaaa
      601 atccttatgc aacgctatat tttggataaa gcaaaattcc acctgtcgcg aacccaaagg
      661 ggttctgtta aacttttaaa tccgttgtta aaccaaagcg ccctgacacc cgaggtctcc
      721 aagataatgt aactattttt cactaaattt actcaaattt acacaaaaaa tgataatact
      781 aagcaatgag catcccttat ttttttgtat ttaatacgtg ttttttttag cttattgaac
      841 acgatactca gctaagctaa gttcaaatta ctccgtgctt acagttctat tcttcgccca
      901 ctcgcgaagc caggcgattc tttctcacat tgtcacagaa agcgggtgag taagggaaat
      961 ctcttcaaga agcatttgag tagatagctc tgttttagca gaaaaggcac tatagagacc
     1021 cgaaacaata acctgctcaa tctcagaacc gctaaaacct tcggttgtct ctactaaaag
     1081 tgtgacatta aacagatgtg gctgcttgcc acgcttttta aggtgtatgt cgaaaataga
     1141 tttccgtgcc tcgggatttg gaagatctat gaaaaatatt tcatcgaaac gtccctttct
     1201 cagaaactca gggggaagct tcataacgtc attcgctgtt gcaacaataa aaacatctcc
     1261 tttcctatct tgaagccatg aaagaaagga cccaaaaatt ctcaatgaaa cgccattgtc
     1321 ttcagtggta tcagatgaag agaatgcctt ctctatctca tcaatccaga ggattatagg
     1381 cgccaatctt tctgcagttt tcattgcttt tttaaaattt ctctcgcttt caccaatgta
     1441 cttgttatat aggttagagg ggtcgagttt aaggagcggt agtccccact ccatagcaac
     1501 cgctttagca cataggcttt taccacaacc cggaactacg agtaaaagga cacccttggg
     1561 aaatgaaagg ccaaagtctg aagctctttt cgggtccgta acgatattac gacgcttggc
     1621 taaccaagac tttaaaccta aaacatcagc tatgtcagcc atactttctt ctaccggata
     1681 atattcaagc agtccctctc tttctacgat accctttttc gcctcaacga cttgtcttat
     1741 atcctctggg gatagtctgc catcttcaac aatgagcatt gtgaaaatct tttcagcttc
     1801 cataagggta agtcccttta tgttgttcaa aagtctgttc gtatcttcag gcgatatttc
     1861 aacctttacg ttcatcctta cattaaggtc tcggattatg ttattaagaa gttcactgta
     1921 ttcttcaatc cgaggggcga ttagctctat tttagcactt atgggtctta agatatccgg
     1981 aatattatca tcaccagtga taatgatcgc gccgcttcgc ttagagaaat taatagctgc
     2041 atccttaagt tttgagacga taagcttatc ttcgagataa tctcccagcc cttggaaatg
     2101 gtaaatagct ggcaactgtg agtgttcgat atgagtaagg gcaatagaag gatcggctga
     2161 gacatatacg tgacccttta cctcttcccg tccgagcccc ttggtgcgcg tccaggaaaa
     2221 aaaagggagt ctcagatgat cggcaaggtg tttaagtaat gtctctgcac gctcctcttc
     2281 aacagtatct agaaaaataa tacagtaacg agatagtatt aggagttcaa tatcctttaa
     2341 tgggtctctt tcattcatac caacatgtat ttataagcta atttcaaata tatacaaaat
     2401 tatgtcttgt tttaataatg tcaaaaagta ttcagtgata gaatctctta atcaatattt
     2461 ttatttatat tagtaaatta gagttcgtta gtagctgact agtttaagaa agtacgacaa
     2521 aaaaggcata tagagaatgt tacttaatca ataaccctgc aagctgcagg gtatggtctt
     2581 aatattaaag cgccacaaca gtcattgcga ggaacccttt gcctagctca ggataaactt
     2641 cgcgacgaag caatctaaat acgagattcc tcgcttcgct cggaatgaca ggaagaaaga
     2701 ttgctacacg gagcctgcac tgagcacttc ggtttcctca gtacaggctc cgtgaacgtg
     2761 ttcgcaatga caatacagca ttgtggaaat tctgtagcaa gctacaggga attatcaagt
     2821 caaagataaa taagtttttc ctagtaaatc tgaatcaagg attttttaaa gcaattttag
     2881 atgcatattc agtcattatt acttatcagc acttgccaac tcaataacaa cctgctttag
     2941 cccggcaact accctcgcca ttcggttgta atcaatctta tcaggcgtat cccactgtgt
     3001 gtgataatac ggataccgga atagggctgt gtcagtaatc ataatagctg aataaccctc
     3061 cttccagaac gaccattgat ctgaccaacc tacaccagga atccagccag gtgcagctac
     3121 accttcagat gggaaatatg tattctttcg aaaaaaagca atgctttgat gaacaaggct
     3181 acgtgaagag gtgttaccga caaagcctat gaaatttcca gtatcaggat aaaaaaaact
     3241 taaaggaaaa ggataacgct gacttccttt cgcatcagag tagtatccta tagtttcaat
     3301 cgaaaacata gcaactatct tttctcgcct tttgtttgca cgacgggcat aaacgagact
     3361 ccccatattt tcagtttgga aaaaaggcgg ttcttcgttc ataaaggcta caaggcgcac
     3421 tgtacgttgg agatttcccc ctttaagaag acgggcaaac tcaagaatag cagcaacacc
     3481 cgtggcatta tcattcgcgc ctggacagtt aataaccgaa tcatagtgtg caccaagaat
     3541 aatgatttca cccggctgag atttacctaa aatttcgact tcgatattct ttgcgatctt
     3601 cccttcgaca acaaaatctt gaaatgacac cccgtagttc aattctcgaa gtgtcttctc
     3661 aatgtagcta atagcagctt ctaggttatt atactccctg aggttgcgct ccccaatttc
     3721 ccctgcaagt acctctacat gctttctaat atgactactg atttcagttt cctcctcagt
     3781 taaaggagtg aattgtcccg agtacgaacg gccaggcatt tttattgata tgccacgagt
     3841 tcctatcatg ttaaaaattc ccaaaaaaat tatgaggatc aaagagccta acgtgaagta
     3901 taagaatcta tggcaaaaca gaagtctatt tggcgaaaga ttcatgcaat tctaaacaat
     3961 gggtgtgata cttcatcatc agcgcctgtc atcttttaat gtaaaatcag taatagatga
     4021 acctctcttt ggattctcaa aaaaccatag cacacaccct attaggtact tcttaaagta
     4081 aaagcttgaa gcattgagca ttcacataaa actatatctc ggcaagaatc gtagcttgaa
     4141 aaccaatgga tgtcacgcca gtagacccag tacctccaga gcttgtaagc acctcaaccc
     4201 ttacttctac ttgtccagat tcatcatccc agtcagcatc aacagattca acagaccaat
     4261 tactattaat tattgatggt ccagcagcac cggaaaatag aagaatccta ttcaaggatg
     4321 ccgtcgcaat tgcacggaca aactggcgtc gtgtaactgt aggaccgatc aaaaacgtaa
     4381 aggtttcttt aatagtcgtg ttagtagata aattggttgg tataattcca gttatgacaa
     4441 ataatcggtt cgcaccatca gggccactga ctacagcctc aatttgctgt agattcacaa
     4501 tgttagtaat tttaataggc atctcaagtc tcctttacta taaggttggt gtattatatg
     4561 tcaaaccaat gaatttagtc aaagaatggt gatcgagccc acaaacttaa ttgtgaatta
     4621 ttatacagag gataacctgc gaatgttaac tatcgtttat ataatatcgg cctatagttt
     4681 gtctctaggt aagccgaaat ccctggcttc ttataaaaaa cctttgtatt cataagcaat
     4741 ttccaaaaga taccatattg ctaacaaaac catatgaggt tagatttaat tcgcatatat
     4801 taaacaaact aataaagatg gaagataaaa taagagaagc caaagaaata ttggacaggg
     4861 catcaaatat aaccgtcctc actggcgcag gcatctcagc agaaagtgga attccaacat
     4921 tcagaggaga agaaggcctt tggaaaaagt ttaggtcaga agaacttgca agccaggagg
     4981 cattccaacg aaacccaaag ctcgtctggg aatggtacga ttggagaaga ggcattgcaa
     5041 aagatgcaaa gccaaaccta ggccattatg cacttgttaa cctagaaaat gagaaatcca
     5101 atttcaccct tgtaacgcag aatattgatg gtctacacca actagctgga agtagaaaca
     5161 taattgagat gcatggaaat caatggcaga taagatgtac tagctgtggt attatagaac
     5221 aaaaccacga tgtacctctc aacgagctcc cgcccaagtg taagaattgc ggtgcactag
     5281 gtcgtccaaa tgtagtttgg ttcggagaga taataccgat ggcaataatc gacaacatac
     5341 ttattgccat agaaagatgt gaagtaatgc ttattgttgg tacatccggt gttgtagaac
     5401 ccgcagcatc aatgggactt atagcaaagc atactggaaa gaccgtaatt gaggtaaatc
     5461 tagagacaac tcccaactca tcactttatg acattagtat tcttgggaaa tcaggagaaa
     5521 ttttgccgat tctttgtaaa tcgaattagc ctgtattcct ctttctttta ttttgtacaa
     5581 gcctaacagc cacttttatt gcggcaatca tgctcgaagg attagcttta cccctccatg
     5641 caatgtcata tgcagtaccg tgatctggtg aggttctgat aatcggaaga cccagagtta
     5701 cgttcacacc atcatcaaac gatagcattt taaatggaat taaa
//
LOCUS       DATDRL010000182         4383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3549676, whole genome shotgun sequence.
ACCESSION   DATDRL010000182 DATDRL010000000
VERSION     DATDRL010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3549676"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..1360)
                     /locus_tag="VI935_06290"
     CDS             complement(317..1360)
                     /locus_tag="VI935_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25244.1"
                     /translation="MKRMLIIGNEHPGIKSVPWTEVTTELIKEITDFQVLLIDLDNYS
                     PIITETQIEAFGTSVVSLVNSRYPVVYLLPRINFSTPNNKLFPFDLSIEEKFGVTLEF
                     NKDDEIIRFYKTFVTKHEIILTGIKEEYFYRTQFRPTCIEPLIRNNIKELCGIKLDRL
                     YLLHPPDRKYHKQAIKSLVEYFSPDIEEEKEEKQEWVSQYESVELGLDKLEAEIKEVE
                     LQVEKLIERKEEKEATKEKIAKWSDLLTRQGKTLELRLKEAFEFLGVEKVEHEPKGSH
                     GPDLVIHHNSIGLTIEVEGSKGPIRIDKARELLQWIADAPPEHKGVLIGNPFRELPPK
                     ERPPETIGFSLRK"
     gene            complement(1423..2349)
                     /locus_tag="VI935_06295"
     CDS             complement(1423..2349)
                     /locus_tag="VI935_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009556277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLE25245.1"
                     /translation="MLCIYYLPDEREVEASEAETILQASLKASIPHAHACGGNARCST
                     CRVLILEGVEHCAPRNTKEKELADRLRFCPEIRLACQTTISSNVKLRRLVLDDEDVEL
                     TSQLRVEATPTSVGEEKHVAILFADIRGFTTFAEALPPYDVIYVLNRYFHQMSQVIYR
                     NSGYIDNYMGDGLMALFGVEDPNGAALKAIKAGVEMLEAVERLRPYLEAVYTKSFQIG
                     IGVHYGEVVVGTIGATNMKKVTAIGDAVNFASRIEAANKQAGTKFLISEETYNQVKDQ
                     VQAVQKCISATLPGKSGEYTLYEVIGINESNP"
     gene            complement(2500..3078)
                     /locus_tag="VI935_06300"
     CDS             complement(2500..3078)
                     /locus_tag="VI935_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HLE25246.1"
                     /translation="MKVYLSKTVVISLIFICSLLIPSYAKEIYKWVDKDGLVHFTDNP
                     SSIPEEKKDEAIIIGEIGDGTDEEVELEVLEDAPNQPEEDNSQQDLEEERKEEAIRQL
                     WRSRALEIEAEERMILEEIEIKKKLISAKKSEVDFLLIYGYFADYSILELRNLQDYLK
                     GLQGQLRLFEEEKAQLKEEARRAGVPPGYLRP"
     gene            3258..3899
                     /locus_tag="VI935_06305"
     CDS             3258..3899
                     /locus_tag="VI935_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HLE25247.1"
                     /translation="MELIKDFTDILIHLDKYLDSIIRSYGGLTYGILFLIIFCETGLV
                     VTPILPGDSLLFATGTFAALGSLDVGLIIILLSIAAIAGDTVNYWIGYLVGPKVFSKE
                     KSRLFNKEYLDRTHHFYQKYGGKTIIIARFIPIIRTFAPFVAGIGRMTYKRFIIYNIL
                     GGIGWIVTFVLGGYFFGNIPLVKNNFALIILAIIIISILPAIIEYLRQRHQSS"
     gene            4048..>4383
                     /locus_tag="VI935_06310"
     CDS             4048..>4383
                     /locus_tag="VI935_06310"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HLE25248.1"
                     /translation="MKKDVLETLIDWIKEANKIVVLTGAELSAESGVPDFSDAKFNPN
                     INDFRSSRDVRAQYWKKIKEIYPSLANAQPNPAHEALVELEMIGKLDCLFTQTTDGLH
                     HRAGQSTVIE"
BASE COUNT         1230 a          908 c          743 g         1502 t
ORIGIN      
        1 attaactttt cctcgaaatg gcagtgaatc ccatatagct gatgcagtct tagtctcgtg
       61 tagatttgct aaaacctcaa tctcacctga aatgatttta acccttttca tcctagttca
      121 tttatatgga atatttggca aatgaatggt ttgatctata attttcttta attaattccg
      181 gtttctcctt tactatttaa aatcttatca agaattttat cgatatttac ttcttcgcct
      241 ttcaattatt tacagactaa tttaaaaatt tcatgcgaat taattaaaac aagggttctt
      301 ttttgtgcta agtcttctac ttccttaacg aaaatcctat tgtttcaggg ggtctttcct
      361 ttggtggaag ttcacgaaaa gggtttccta taagaactcc tttatgttca ggaggggcat
      421 cagcaatcca ctgtagcagc tcccttgctt tatctattcg gattgggcct ttagaccctt
      481 caacttcaat tgtaagccca atactattat gatgaattac taaatccggg ccatgggacc
      541 ctttaggttc atgttcaact ttctcaactc caagaaattc aaaagcttct tttaatctaa
      601 gttctagtgt ttttccttgg cgagtaagta aatctgacca tttagcaatt ttttcttttg
      661 tagcttcttt ttcttctttt ctttcaatta atttttcaac ctgcagttct acttctttta
      721 tttcagcttc taatttatca agtccaagtt caactgattc atattgagat acccattctt
      781 gtttttcttc tttttcttcc tcaatatcag gagaaaaata ttccaccaaa cttttgattg
      841 cttgtttatg atactttcta tcaggaggat gtagtagata taatctatcc aattttatac
      901 cgcatagctc cttaatgtta ttccgaatca aaggttcaat acaagtcggt ctgaactgag
      961 tacggtagaa gtattcttct ttaattcccg ttaaaattat ttcgtgttta gtaacaaacg
     1021 ttttataaaa ccttataatt tcatcatctt tattaaattc aagagtgaca ccaaatttct
     1081 cttctattga taggtcaaaa gggaatagtt tattattagg tgtagagaaa ttaattcttg
     1141 gtaaaagata gacaacagga tatctactat ttactaaaga cacaacactt gttccgaaag
     1201 cctctatttg ggtttcagta ataattggag aataattatc caaatcaatc aataaaactt
     1261 gaaaatcggt aatctcctta attaattcag tagtaacttc tgtccagggt accgatttta
     1321 ttccaggatg ctcattacct ataatcaaca ttctcttcat tttctttgat ctcttatgcc
     1381 ctgataacct aagatactga cctaaatgac tttcttcaga acttaagggt ttgactcgtt
     1441 tatcccaatt acttcataga gggtgtattc accgctcttt ccaggtagcg tggcgctgat
     1501 acatttttga accgcctgga cttggtcttt cacctgattg taggtttctt ctgagatcag
     1561 aaactttgtg cccgcctgct tattcgcagc ctcaatgcgg ctagcgaagt taactgcatc
     1621 accgattgct gttaccttct tcatattggt ggcaccaatg gttcctacta caacctctcc
     1681 ataatgcaca ccgatgccaa tctgaaaact cttcgtatat acagcctcaa ggtatggtct
     1741 taatctctca actgcctcta gcatctcgac tcctgccttg atagctttta gagctgctcc
     1801 gttgggatct tcgaccccga ataaagccat gagcccgtct cccatataat tatctatata
     1861 accgctgtta cgataaatca cttgactcat ctgatggaaa tagcgattta gcacatagat
     1921 gacatcatat ggaggcaagg cctcagcaaa ggtggtaaac ccgcgtatgt ctgcaaatag
     1981 aattgcaaca tgcttttctt cgcccacaga ggttggtgtt gcttctactc tcagttggct
     2041 agttagttct acatcctcat catccagaac gagccgtcgc agtttaacgt tacttgagat
     2101 cgtagtctgg caggcaagtc gaatctcagg gcagaagcgt agacgatcgg cgagttcttt
     2161 ctccttggtg ttgcgagggg cacaatgttc tacaccttca agaattaaaa cgcgacaggt
     2221 agaacaacgg gcgttcccac cacatgcatg agcatgtgga atactggcct ttagggatgc
     2281 ttgaagaata gtctcagcct cacttgcctc aacttctctt tcgtcaggta gatagtatat
     2341 gcacaacatt gtatgaactt ttcaaaatta agagataatt ctgatccgct ttttctattc
     2401 ccaagtatga attaccgatt ctagtctaca ctattatatc aaatctagtt ctgtttgttg
     2461 taggaagaaa ttttatttca tgtctactca actatatttt tatggtctca aatatcctgg
     2521 tgggactccc gctcgacggg cttcttcttt aagttgtgct ttctcttctt caaataacct
     2581 caactggcct tgaagacctt ttagataatc ttggagattc cgtagctcta gtatggaata
     2641 gtcagcaaag tatccgtata taagcaagaa atcaacctcg cttttctttg cactaattag
     2701 cttcttcttg atttcaattt cctcaagaat cattctttcc tcagcctcta tttctagtgc
     2761 acggcttctc caaagttgcc ttatagcttc ttcttttctc tcctcttcaa gatcttgttg
     2821 actgttgtcc tcctctggtt gatttggagc atcttccaaa acctcgagtt ctacctcctc
     2881 atctgtgcca tcgccaattt ctccaataat gatagcctca tcttttttct cctcgggaat
     2941 gctagaaggg ttatctgtaa agtgaactaa tccgtcttta tcaacccact tatatatctc
     3001 ctttgcataa gatggaataa ggagggaaca aataaaaatg agcgaaataa ctacagtctt
     3061 acttaaataa accttcataa atgaatgttt agattcgtga aaattctcta gctctattac
     3121 aacaatacac ctgcacaagt agaagttcaa tttttatctt gtcctagtag gcaatggata
     3181 aaataagaaa acagtttaat gcttgtgaaa tcgtattatt taatttaaat tatgccccta
     3241 caatttaagg gtatttcatg gaattaataa aagatttcac cgacatccta attcatctcg
     3301 ataaatatct tgattcaatc ataagaagtt atggcggctt gacatacggg attctttttc
     3361 taatcatatt ttgtgagaca ggattagtgg taacaccaat cctcccagga gattcccttc
     3421 ttttcgctac tggcaccttt gctgcccttg gctcactaga tgttggcttg ataataattc
     3481 ttcttagcat cgcagctatc gccggagata ctgtaaacta ctggatcggg tacctcgtgg
     3541 ggccaaaggt gttctccaag gaaaaatctc gtttgttcaa caaggagtat ctagaccgca
     3601 cccatcactt ctaccaaaaa tatggtggaa agactattat tatagcgaga tttattccaa
     3661 ttattcgtac ctttgcaccc tttgtggctg gtatcggtcg catgacgtat aaaagattca
     3721 ttatctataa cattcttggc ggtattggtt ggattgtgac ctttgtttta ggaggttatt
     3781 tttttggcaa tatcccgtta gtcaaaaata attttgcgct cataattttg gctataatta
     3841 tcatctcaat tctaccagcg ataattgaat atctgcgtca aaggcatcaa tcctcataat
     3901 tagggtttcg tgcattgtga tttgtgattc gaaatatcat gcacgattca cgaaccacaa
     3961 gtcatcgtca acgaattcgc tatttttatg gcatactata aacgtttgat tgacattttt
     4021 tcgtcctgaa tagtataatc catgtaaatg aaaaaggacg tattagagac tctaattgat
     4081 tggattaaag aagcaaataa gatagttgtc cttactggag ctgaactatc tgccgaatca
     4141 ggcgtccccg atttttctga tgcaaagttt aatcctaata taaacgattt tcgttcaagt
     4201 agagatgtaa gagcccagta ttggaagaag attaaagaaa tttatccctc acttgcaaac
     4261 gctcaaccga atccagccca tgaagcgctt gtagaacttg agatgattgg taagcttgac
     4321 tgccttttta cccaaacaac agatggactt catcatcgtg caggacaatc aacagtaata
     4381 gag
//
LOCUS       DATDRL010000183         5694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2403749, whole genome shotgun sequence.
ACCESSION   DATDRL010000183 DATDRL010000000
VERSION     DATDRL010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2403749"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VI935_06315"
     CDS             <1..346
                     /locus_tag="VI935_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HLE25249.1"
                     /translation="FFATNRDPVFPTPEGLVPATGAILASIEYATGKKATIVGKPEVI
                     IFEVAKKFLSSHERVAIVGDRLDTDILGGKRAGITTIFTLSGSIDQDELSKSDIIPDY
                     VINDLKDLLKEQ"
     gene            1050..4253
                     /locus_tag="VI935_06320"
     CDS             1050..4253
                     /locus_tag="VI935_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HLE25250.1"
                     /translation="MSQNSKTDLTFFTNEPGHTLLDRFIATLKGVQFFDIIVGYFRIT
                     GFYQLYESLEPVERIRILVGLSVDRKTHELMLESGKQQELDFESSKRTKQILVEYLRQ
                     EMDGSEDSYNTEVGVKRFIEFLQSGKLDIKAYPSSRLHAKVYISRFSEDDRDFGRVIT
                     GSSNFSESGFVDNLEFNVELKNSADVKYALSKFEELWKDAVDITEEYVGTISNQTWLN
                     DRITPYELYLKFLYEYLKEEINIDDEEVETYLPEGFKELKYQKQAVISARKILEAYSG
                     VFLADVVGLGKTYISALLAQQLNGKFLVICPPILVEYWRDTFRDFGVRGFDVESMGKL
                     DRILKRGADKYDYIFIDEAHRFRNEGTQGYEKLHQICFGKNIILVSATPLNNTIDDIY
                     SQLKLFQVPKKSIIPGLTNLEKYFQSLKSKLNPYKKSDPEYTKVVKEISKDVREKILK
                     HVMVRRTRTEVSKYYHDDLVKQNLSFPELDDPKRIIYTFDGKTNVVFNETIELLKRFI
                     YSRYTPLLYLKKELTEFEKQSQRNVGGFMKGILVKRLESSFHAFRNTLRRFIDSYQKF
                     IEMFNNGTVYISKSVDVYEYLDSDNEEALIEMVTGGRVQKYGAEEFRDEFKVHLSNDL
                     KVLLKIQSLWKDIEDDPKLEKFISELKAKSYLKKNKLVVFTESMETGEHLYRSLNDEF
                     PNEVLFYSSQRGMNKDLNISAGTARDLIKENFDPNCSPQREDVRILISTDILAEGINL
                     HRSNIVINYDLPWNPTRVLQRVGRVNRIGTDYKKIFVFNFFPTAQSDEHLGLEANIKA
                     KIQAFHDTLGEDAKYITEEEETSTHELFGDALFRKLNDRRSYEGEEETDSELKYLKIL
                     REIRDRSPELIEKIKHLPKKARSSRKISRKEKNQLVTFFRKGKLKKFLLAVNDDAKEL
                     SFFEAVDLFECEPDNRKQAIPSDYYKLLDLNKKEFEYLTSEESPGHLASRSGSNEGYV
                     IRRLKAKEMSSYTGFTEDDEGYIKSVLRAFEYGIIPKRTSQILKRELERESNPLKVLS
                     TLRSKIPDNLLEPVSNTHEKTHDKAEVILSEYLIAGD"
     gene            4255..>5694
                     /locus_tag="VI935_06325"
     CDS             4255..>5694
                     /locus_tag="VI935_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HLE25251.1"
                     /translation="MTKEEAKRLIEETFKNPFDESQFRRFVKNLLNDINESKAFEFHG
                     AYIPDAYGDYIRQYKRLGQYTDPKGNVLDVLTVRLMKESSLDRARTMQRNFIATYFKR
                     RGDDKDNALVAFYHDDLDDWRFSYVRRDYRLEQTESGKVKIREELSPARRYSFLVGTT
                     EPSHTAQQQLVPILQDDRNNPTLKQLEEAFNIEKVAKEFFEKYRGLYLKLNEELDELV
                     KNDQKIREEFTTKNIATENFAKKLLGQIIFLYFLQKKGWMGVEKDASGNYKPWGAGPR
                     DFLRKLYEKKYINYKNFFNDVLEPLFYQALAIDRGKVSEFTQFKCKIPFLNGGLFEPI
                     GGYSWEETDILIKNETFAEILHTFDLYNFTVREDEPLEKEVAVDPEMLGKVFENLLEV
                     KDRKSKGTYYTPREIVHYMCQESLINYLDTALNTHQEPFVKQRPVQQKLLGNPDPEQL
                     GLTMSDYKPTVPKEDIEEFIRKGELIIQHD"
BASE COUNT         1944 a         1079 c         1113 g         1558 t
ORIGIN      
        1 cttctttgca acaaatcgtg accctgtttt tcccacccct gagggactcg tgcctgcaac
       61 aggtgccata cttgcatcaa tcgagtacgc cacagggaaa aaagctacca tagtcggaaa
      121 gccagaggtt ataatatttg aggttgcaaa gaaattcctt tcctctcacg aaagagtcgc
      181 aatcgttgga gatagactcg acaccgatat acttggcgga aaacgcgcag gtattacaac
      241 tattttcacc ctttccggtt ctatagatca agatgaactt tctaaatcag acattatccc
      301 tgactacgtt attaatgatt taaaagattt attgaaagag caatgatgaa cggctttcat
      361 ttataatgtc tccacggcat ctaaaccgga ttataaaagc taataatgac acccgctcaa
      421 agtccttacg aatcacgtcc cattaatttg ccaaattcta tccatcaatc taagataaga
      481 aaccaccctg aaatttaaaa aggttgaatt ttgttgtatt tttttcgtat catttttgga
      541 gtttttgtat tactataaat tgaggtagtt atggataata ttaaaaaatt tcaagaatta
      601 cttaaggagc tcttccaatt tgagagaaaa aaaggggtca aatctttcgt tgactaattt
      661 tgtactgaat ccatatcaac tttttgctga atttttatag gaccctgtct taatccacgc
      721 tttattgcac gctcactcaa ctgcaacata ataactacct attccaaacc tttcagataa
      781 ctctgtttat ataagatcgc ctatactctt aactaaatac atccaaacca ttggaatatc
      841 ttctgttggg caatcccaca attggatatt gaattcttag tatcttatac atttaagtag
      901 agtctggata aaataataat atatcaacaa aagacttaac cccatccctc atcatgacat
      961 cttccatcgt ttgtagtaat ctataaatta ttgacttttc tctgctattt ctgagataat
     1021 tgtaattaat ttagttaaac gcaaatctta tgagccaaaa ctcaaaaact gatttaacct
     1081 ttttcacaaa tgaacctgga catacccttt tggatagatt tatagcaact ctaaagggtg
     1141 ttcagttttt cgacataatt gttggttatt ttcgtatcac cggtttttat caactatacg
     1201 aatccttgga gcctgttgaa agaattagaa tcctcgttgg tcttagtgtt gacaggaaga
     1261 ctcatgaatt aatgttggaa tcagggaaac aacaagaact tgactttgaa tcatctaaaa
     1321 gaaccaagca gattcttgta gaatatttaa ggcaggaaat ggatggttca gaggacagct
     1381 ataataccga agtcggtgta aaaagattca tcgaattctt acaatcagga aaacttgaca
     1441 ttaaagcata tccaagctcc aggctgcacg ctaaggtgta cattagcaga ttttcggaag
     1501 atgacagaga ttttggcaga gtcataactg gctcaagcaa tttttcagag tcaggctttg
     1561 tggacaatct cgagttcaat gtcgagctaa aaaatagcgc tgatgttaaa tatgcccttt
     1621 caaaattcga ggaactctgg aaagacgcgg ttgatattac agaggaatac gttggtacaa
     1681 taagtaatca aacatggctc aatgatagaa taactcctta tgaactgtat ctaaagtttc
     1741 tctatgaata cctcaaagag gaaataaata tagatgatga agaagtcgaa acctaccttc
     1801 cagaaggatt caaagaatta aaatatcaaa agcaggctgt aatatcggct agaaaaatat
     1861 tagaagccta tagcggggtt ttcctagctg acgttgtggg acttggcaaa acttacattt
     1921 cagcgcttct cgctcaacag ttaaatggga aatttctcgt tatttgccct cctattttgg
     1981 tggaatactg gcgagatacg ttcagggatt tcggagttcg tggtttcgat gtggaatcta
     2041 tgggcaaact cgatagaatt ctaaaacgag gtgctgataa atacgattac attttcatag
     2101 atgaagccca caggtttcgt aatgaaggca cgcaaggata tgaaaagcta catcagatat
     2161 gttttggtaa gaatattatt ttagtctccg cgacaccact taataacact attgatgaca
     2221 tttatagcca gctaaagctt ttccaggttc ccaaaaagag catcattccc ggtcttacta
     2281 atcttgaaaa atattttcaa agccttaaaa gtaaactaaa tccttataaa aaatccgacc
     2341 cagaatacac taaagttgta aaggaaatat caaaagatgt cagggaaaaa atactgaaac
     2401 atgtaatggt acgaaggaca cgcactgagg tatccaagta ttatcatgat gatctcgtga
     2461 aacagaatct ttctttccct gagctagatg accctaaacg tatcatctat acgtttgacg
     2521 gcaaaaccaa cgtagttttt aatgaaacta tcgagctcct taaaagattt atctattccc
     2581 gctatacccc tcttctttat ttgaaaaaag agcttactga gttcgagaaa cagtcccagc
     2641 gcaacgttgg cggtttcatg aagggaatac ttgtaaaacg acttgaaagc agtttccatg
     2701 catttagaaa taccctaaga cgttttatag attcctacca aaagtttata gaaatgttca
     2761 acaacggaac ggtctatata agcaaaagtg tggatgttta cgaatattta gatagtgata
     2821 acgaagaagc tcttattgag atggtaacgg gaggcagggt tcaaaaatac ggggcggaag
     2881 aatttagaga cgagtttaaa gttcacctaa gtaatgattt aaaggtactc cttaaaatac
     2941 agtcgctatg gaaggatatc gaagacgacc caaaactcga aaagtttatc tctgaattaa
     3001 aagcaaaatc ttatttaaaa aagaataaat tagttgtatt cacggaatca atggagacgg
     3061 gggagcattt gtataggagc ctgaacgatg aatttcccaa cgaggtctta ttctattcga
     3121 gtcagagagg tatgaataaa gacttaaaca taagtgctgg aacagcgagg gatttaatta
     3181 aagagaattt cgatccgaat tgttctccgc aaagagaaga tgtacggatt cttatatcca
     3241 cagacattct tgccgagggt atcaacctcc acaggtcaaa catcgtaata aactacgatc
     3301 ttccctggaa tccaacaagg gtccttcagc gtgttggacg tgtaaacaga ataggcacag
     3361 actacaaaaa gatttttgtc tttaatttct ttcccacagc ccagtctgat gaacacctgg
     3421 gactcgaagc aaacattaag gctaaaatac aggcttttca tgataccttg ggagaggacg
     3481 caaagtatat aactgaggag gaggaaacct caactcacga gcttttcggt gatgcactct
     3541 tcaggaagct aaacgacaga agaagctatg aaggcgaaga ggaaacagac tcggagttaa
     3601 aatacctcaa aatactcaga gagattagag acaggagtcc tgagcttatt gaaaagatta
     3661 aacacctgcc gaagaaagcc cgctcatcac gtaagattag tagaaaggag aaaaatcagc
     3721 ttgttacttt cttccgaaag ggcaagttaa aaaaattcct gcttgcagtt aatgatgacg
     3781 caaaagagct tagtttcttt gaagcggttg atctctttga gtgcgaacct gataaccgca
     3841 agcaagctat tccatccgac tattataaac ttctggattt gaacaagaag gaattcgaat
     3901 atctaacatc tgaagaaagc cctggacatc ttgcatcaag gagcggttcc aacgaggggt
     3961 acgttattag aaggcttaaa gctaaagaaa tgtcctctta cactggattt actgaagatg
     4021 atgaaggtta tatcaaatca gttctaagag cttttgagta cgggattatt cccaagagga
     4081 caagtcagat tttaaaaaga gaattggaga gagagtcaaa tcctctcaag gttttatcaa
     4141 ccttgagaag caaaatacca gacaatcttc ttgaacccgt ttcaaatact catgaaaaga
     4201 ctcatgacaa agcagaagtt atcctgtccg aatacctaat tgcaggtgat taggatgaca
     4261 aaagaagaag ctaaacggct gatagaagag actttcaaaa acccttttga tgaatcacaa
     4321 ttccgcaggt ttgtaaaaaa tctcctcaat gacattaacg aatccaaagc ctttgagttt
     4381 cacggcgcct atatacccga tgcatatgga gattatatca gacaatataa acgtcttggt
     4441 caatacaccg acccgaaagg aaatgtttta gatgtattaa ctgtaagact catgaaagaa
     4501 tcctcactcg atcgcgcccg aaccatgcag aggaatttca ttgccaccta cttcaagaga
     4561 agaggagacg acaaagataa tgctttagta gctttctatc acgatgattt agatgattgg
     4621 cgtttctcgt atgtccgaag ggattaccga ctcgagcaaa ctgaaagcgg caaagttaag
     4681 attagggaag agctaagccc agcccgccgc tattcgtttc ttgttggaac aacagaacct
     4741 agccacaccg cccaacaaca actcgttccg attcttcagg atgatagaaa caacccaacc
     4801 ctcaaacagc ttgaagaagc attcaacatt gaaaaggttg caaaggaatt ctttgagaaa
     4861 tacaggggcc tttatctaaa actcaatgaa gagctcgatg aacttgttaa aaatgatcaa
     4921 aaaatccgag aagaatttac aacaaaaaac attgctacag agaattttgc aaagaagctc
     4981 cttgggcaaa tcatatttct ctacttcctt caaaaaaaag gctggatggg agttgaaaag
     5041 gatgcaagtg gaaattataa gccctggggt gcaggtccaa gagatttcct gcgaaaactc
     5101 tatgaaaaga agtacatcaa ctacaaaaac ttctttaacg atgtccttga acctcttttc
     5161 tatcaggcgc ttgcgattga cagaggaaag gtatccgagt ttacccagtt taagtgcaag
     5221 attcccttct taaacggcgg actcttcgag ccaattggag gatatagttg ggaagaaacg
     5281 gatatcctta taaagaacga gacctttgcc gagattcttc atacctttga tctttataac
     5341 ttcaccgtcc gcgaggacga gccgctcgaa aaagaggtag ccgttgaccc tgagatgtta
     5401 ggaaaggtgt ttgagaatct gcttgaagta aaggacagga aatcaaaagg cacatattac
     5461 acaccgcgtg agattgtcca ttacatgtgt caggagagct taattaatta tttagatacc
     5521 gctctcaata ctcatcaaga accctttgta aagcaaaggc cggttcagca aaaactctta
     5581 ggaaaccccg acccggaaca actgggttta acaatgagcg actataaacc aactgtgcca
     5641 aaggaagata ttgaagagtt tatacgaaag ggcgagttaa tcattcaaca tgat
//
LOCUS       DATDRL010000184        12925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_603906, whole genome shotgun sequence.
ACCESSION   DATDRL010000184 DATDRL010000000
VERSION     DATDRL010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12925
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603906"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            587..1633
                     /locus_tag="VI935_06330"
     CDS             587..1633
                     /locus_tag="VI935_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HLE25252.1"
                     /translation="MYQNKNEKIKSRTVACLLPLSGKYELIGKKALRGILTARNLFQS
                     ENDTQIIVKDYGDSDLELKKAIDEVVTKDNASVVIGPLLSSSIREVSSSVKSLRVPTV
                     VFPLSKEVSLENPYIISFSYPVEKQAQVLARYAVQDIKITSLGILYPRSDLGELFKEA
                     FIKGTKEFGGEIIYIGSYDPKLLDILTEIGWISSRRPEAIFIPDAATHSGEIVLKLRQ
                     ENGLEDLILLGPNTWNSRSFLKTVGFDVDGTIFTDFFFSGSERWIDFSNKFRAAFHEE
                     PEFFEYQVYEAASLIFHVLQNPIQRREQIKERILSLRDNPLFDIAQNIDGSLEISPKP
                     LILTINDGEIVRVK"
     gene            complement(1748..2926)
                     /gene="larC"
                     /locus_tag="VI935_06335"
     CDS             complement(1748..2926)
                     /gene="larC"
                     /locus_tag="VI935_06335"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HLE25253.1"
                     /translation="MKRLYFDCFSGASGDMILGSLIHLGVDLENLRSELSNLPVKGYK
                     ISARFVNRSGIKAYKFNVTPGKDKTERNFKEIRSIIQRSKLNDKVRKTAVLIFEKLAE
                     AEAKIHGVKPEEIHFHEVGGIDSIVDVIGTTIGINLLGIEEIYSSAIPFSRSLIKMEH
                     GLYPGPSPAVLELLRGVEMYESERNEELVTPTGAAILKVISKGFGSFPRMRISKIGYG
                     AGTRNSHEYPNVLRAILGEDNINSEGMEKDTVVVIESNIDDMSPEAFDNLFDKLLLLE
                     EALDVSVVNAMMKRGRPGHIVKVISKEGGAQKVTETLFKESTTLGVRYTEANRFKLRR
                     KERIISTRYGDIRVKIVDEPELGIKRAKPEYEDCKSAANRHNVSLQEIYDEVNRVIDR
                     "
     gene            complement(2943..3758)
                     /gene="ccsA"
                     /locus_tag="VI935_06340"
     CDS             complement(2943..3758)
                     /gene="ccsA"
                     /locus_tag="VI935_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HLE25254.1"
                     /translation="MDFHLIISLFYLISSFLYCSHIWTQNQSVSRWGFHTFRIGVALH
                     TVDLVLTYLQGDPIAGGLDRSLYSFSWFIAIVYIVSQARFKATVFGAFVAPFAFLMTI
                     PSVILPQGIIEHDPSLRNPWILTHIGLVFLGEALFTIAFIAGVLYLFQEKRIKSKRVG
                     TFLMKLPSLTSLDSLNQVCLFVGFPLLTLGLALGLLSVKEIWGTLWSWGPKETWSVVT
                     WFLYAVLIHGRLSTGWRGRKAALGAVIGFGVVLFTFFVIGYLAPGRHDFLGMY"
     gene            complement(3887..4945)
                     /gene="prfA"
                     /locus_tag="VI935_06345"
     CDS             complement(3887..4945)
                     /gene="prfA"
                     /locus_tag="VI935_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HLE25255.1"
                     /translation="MLRRLEEFEKRYEDIEKVLSNPSISPSEIHRYSKQRSEISEIVE
                     TYREHKSTREEIEKNRVLLGDKEVGELAREEISSLEKKQVILEEKLRLLLLPKDPDDT
                     KNVFIEIRAGTGGEEAALFAADLFRMYTRYAERNGWKVEIMDMNETGIGGFKEVIAEI
                     QGKNVWSKLKYEGGVHRVQRVPTTEAGGRVHTSTATVAVLAEPDDVEVTVDEKDLRID
                     TFRAGGKGGQHVNKTDSAVRITHLPTGIVASCQNERSQHQNRVVAMKVLRARLYEIEE
                     KKRESEISNMRRSMVGSGDRSEKIRTYNFSQNRITDHRIGLTLYRLDAALDGDLEELI
                     QALISHYQTEKLKQAVGS"
     gene            5029..5238
                     /locus_tag="VI935_06350"
     CDS             5029..5238
                     /locus_tag="VI935_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25256.1"
                     /translation="METIDILESRIQETLSRKARLEEERRNLVDEVMLLHENIRRLET
                     EKEEIKAKLEAVIEKIELYLSRSEA"
     gene            5241..5579
                     /locus_tag="VI935_06355"
     CDS             5241..5579
                     /locus_tag="VI935_06355"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HLE25257.1"
                     /translation="MRRVKINVLNNELFIKTDAEEEYVQEISNYLEDKVREASPQVDA
                     VVVPRPFLLATLKIVDDYFRLKREYEEFKNRAEERSRRLVEILDSSLGEKETFEYWRG
                     GRRENQKKEV"
     gene            5530..6210
                     /locus_tag="VI935_06360"
     CDS             5530..6210
                     /locus_tag="VI935_06360"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HLE25258.1"
                     /translation="MNIGGEEGEKTRKRRFRINLTSGFEEPALNLSTLQRKRFHVIEE
                     KGDLRLRLLEKRLSLNPKDVWIRSEDIKRRLIKLKSFQEARSIALYYPIRNEVGTQGI
                     FKIAKELGKGVHFPLVYGSSLEFLKINDLAELKIGRFGVPEPSHFSPRVEIENIDLIV
                     IPGIAFDRFGARIGYGKGYFDRTISKIDIKKRVGLAYDLQVVESVPLERYDERVGLIV
                     TETGAIIC"
     gene            6228..7781
                     /gene="rny"
                     /locus_tag="VI935_06365"
     CDS             6228..7781
                     /gene="rny"
                     /locus_tag="VI935_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HLE25259.1"
                     /translation="MMWFIIATTLAFILGALGYYLFRAIREKRNLELKRTEAQIVIEA
                     AKKEAQKIRAEGELQAKEIIEKSKSEADRDYREKQKEVSKIEKRLQSREENIDKKYES
                     IERKETEISRRERETLSKEKVVAEKEKELNSIITEAKKKIEEVAGMSQEAAKRELINI
                     IENEAKHEAAKKLKLIEDELHENAESKSKDIIALAIQKYAGQYTSEKTISVLNLPNDE
                     MKGRIIGREGRNIRSIEARTGVDIIIDDTPETVVVSSLNPIRREVARIALERLIADGR
                     IHPARIEEVVTEVEDEIEKEIQKAGEEAVFDIGAHNMHPELVKLVGKLKYRTSFSQNI
                     LNHSLEVAFICGVIAGELGLNVKTARRVGLLHDIGKALDHEVEGSHVEIGEEVVRKYG
                     EPADVVEAVAKYHDPQPQSIWAILVQASDAISAARPGARREMYETYVKRIEDIERIAS
                     SFRGVEKCYAIQAGREVRVIVESGKVNDEEAVLLSNEIAKKIQREVAFPGQVKITVIR
                     EVRATAYAT"
     gene            8008..8808
                     /locus_tag="VI935_06370"
     CDS             8008..8808
                     /locus_tag="VI935_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HLE25260.1"
                     /translation="MEKKEGLCVLFIGDIVGEPGRNAVKTLLPGLVKDNLVDFVVANC
                     ENAAAGKGITPKITEYLLNTGINVLTSGNHIWDRQEIIPYIETQPLLLRPANYPKGTP
                     GKGHGIFETKTGYRIGVVNLCGKLFMDSYEHPFLTALDLVNELSKHTRMIIVDFHAEA
                     TSEKKAMGWYLDGKVSAIIGTHTHVQTADERVLPKGSSYITDVGMTGSIDSVIGMDKE
                     KAIKRFLTLIPIRLEPATDNVCLNGVLVDLDKDSGKSLGIKRIVVNAK"
     gene            complement(8919..10220)
                     /locus_tag="VI935_06375"
     CDS             complement(8919..10220)
                     /locus_tag="VI935_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLE25261.1"
                     /translation="MSNETIEVSVSKLGVFGWYKDINAQQWKAFIAAFLGWTLDAMDL
                     LLFAFAVSSIATVFKLNPSQVGAIFSVTLFSSAFGGAVFGIVSDYIGRVRALTYTILL
                     YSAFTGLSALSPNVTFLLVCRLFLGLGMGGELASGEVLVAESWPRQHRGKVIGMVQSG
                     WGIGYILAAVLATLIIPYFDVNLGWRILFLVGITPAFLVFYIRRHLDEPEIWKKTADM
                     RKEGKLSEKGQSFTLNQIFRRDLIRYTITATLLTSLCMIAYWGLYFWIPQFLSRPVAE
                     GGIGLGPKGFAWIIPINIGAFIGCNTFGWISDRFGRRPVFVAYLIIMAVLVWFFGHAK
                     DLTTVLILGPFIGFFGTGFYSGFGAIFSEIFPTRARGTAQGFCYNCGRGVSAVAPPLV
                     GYVAGIYGYGLSLITVSVFAVAAAIVVLTLPETRGKKLEIE"
     gene            10565..11893
                     /gene="miaB"
                     /locus_tag="VI935_06380"
     CDS             10565..11893
                     /gene="miaB"
                     /locus_tag="VI935_06380"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HLE25262.1"
                     /translation="MQRKYLYLETYGCQMNEYDSDKIRSLLGMELTNDPKKADVVIIN
                     TCAIREKADQKAFSSLGRLKHLKFRKPKMILGVGGCVAQLYGERLIENFPHLDLVFGT
                     RTITLLPELISQVEREKRRMVETSFDIEEVFEFEPYHEEGKVTAFVSVQQGCNKMCSY
                     CIVPLVKGKETNRSVEDILAETQKLVQKGVREITLIGQTVNSWKMAGYKFGDLLKIMA
                     EIEGLFRIRFTTSYPRDVTKKLIDAIRDVPKVCHHIHLPVQSGSNRILTEMGRTYTRE
                     WYMDILKRIRDAIPEMAITTDIIVGFPGETEDDFNDTMSLLKQAEFDGTFSFKFSPRP
                     GTPASEFSDMVSDQVSSRRLMELQAFQSEITVKKNQSIVGHIEEVLIEGESKNDPSWV
                     TGRTSQNRVVNFPGTTDLRGKLERVIITEGFQNSLRGRLLENEMEVSHVS"
     gene            11883..12359
                     /locus_tag="VI935_06385"
     CDS             11883..12359
                     /locus_tag="VI935_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HLE25263.1"
                     /translation="MFLEMKVSGIALDPFTNTPIVILKDLTGERVLPIWIGFMEASAI
                     AMELEKTPRARPLTHDLMKALLETLKFSVIRVDVTDLRNDTFYAEIRLQRGSEEHIVD
                     SRPSDAIAIALRTGSPIFVRDEVLEKSKKLEFDEEKDKLSDLLEKIPPGDFGKYKM"
     gene            complement(12558..>12925)
                     /locus_tag="VI935_06390"
     CDS             complement(12558..>12925)
                     /locus_tag="VI935_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLE25264.1"
                     /translation="KALETLESYHYPGNVRELENIIARCVALETSNVIRQQTLPELMV
                     NMDSLNIENGIAGNFGLDHLLETFEKRTIEKALAQAQGSKVEAAKLLGITFRSLRYRL
                     AKHGLLEEEEEGADDKENM"
BASE COUNT         4016 a         2434 c         2734 g         3741 t
ORIGIN      
        1 tcaaattgag catttgcaaa cttcaggtta tcttcccata aagaaaggaa aagcttaagg
       61 cttgcaagat aattttcttt cactaagcct gtaaggctct taaaattctc tgtaaaatca
      121 gagactttta tctcctcttt ctcttttttc tccgccattt aaatttacct cctaaattaa
      181 ttttatatta gtataatcat attaaataat ttgtcaaggg tcttcttcgc aaatgcaaaa
      241 ttattatatt ataataattc attaaattaa ggcacttaca atatagggtt atctatttaa
      301 tatggcacag gctaacacta atgggttaga ttcgttacac gcattcaata tttcatagag
      361 tattcgttaa gacttttgta gtgccatatg aactttattc ataaattagt ttattatccg
      421 aaacatcgaa attagatgtt ataattcctg gaaaatgttt ttattcatca atattaaaaa
      481 atggcgagat tatatatgca agcaagccct attgacatta ctctttatgc tttcactcat
      541 tacttattta attttcagtt tcttccctgt ctatagtcag gagatattgt atcaaaataa
      601 gaatgaaaaa ataaaaagta ggacagtcgc ctgtcttctt ccattaagtg gcaagtatga
      661 attaataggt aagaaggcct taagaggaat tctcactgca agaaatctat tccagtctga
      721 gaacgatact cagattattg tcaaagacta tggagatagc gatctagaac ttaaaaaagc
      781 cattgatgag gtcgttacaa aagacaatgc atcggttgtg ataggtcccc ttcttagcag
      841 ctctattagg gaagtgagtt ctagtgtcaa atctcttagg gtaccaacag tcgtgtttcc
      901 gctatctaaa gaagtatcat tggaaaatcc ttacattata agtttttcct accctgtaga
      961 aaaacaggct caagtgcttg caaggtatgc agtgcaggat atcaagataa caagccttgg
     1021 cattctatat ccaaggagtg atcttggaga attatttaaa gaagccttca ttaagggtac
     1081 aaaagagttc ggtggagaaa ttatctacat tggttcatat gatcctaaac ttcttgatat
     1141 tttaactgaa atcgggtgga taagttcaag acgccctgaa gcgattttca taccggatgc
     1201 tgctacacat tcgggtgaga tagtattgaa actgagacaa gagaatggcc tagaggattt
     1261 aatccttctt ggcccaaata catggaacag caggtctttt ctaaaaaccg tgggttttga
     1321 tgttgatgga actattttta ctgatttctt tttctctgga agtgagcgat ggattgattt
     1381 tagtaacaaa tttagagctg cttttcatga ggaaccggaa ttttttgaat atcaggtata
     1441 cgaagcagct agtcttattt ttcatgtttt acaaaacccg attcagagaa gagagcagat
     1501 aaaggagagg atcctttctc tcagagacaa ccctcttttt gatattgctc aaaacataga
     1561 tggaagccta gaaatctctc cgaagcccct tattcttacg ataaatgatg gggagatagt
     1621 aagggtgaag taaggagttt tagaattaaa gttaattaag gcccaagata aggtattagt
     1681 tcggattccc ccccgtaact atataagagt tagaactttc gtctggttag gaaaatcaga
     1741 actacttcta tctatcaatc actcgattta cttcatcata aatttcttga agtgaaacat
     1801 tgtgtcggtt tgctgcgctc ttacagtcct cgtattcagg ctttgctctt tttattccta
     1861 attcaggctc atcgactatt tttaccctta tgtcaccata tcttgtagag ataatccgct
     1921 cttttcttct cagcttaaat cggtttgcct ctgtgtatct tacgccaagt gttgttgatt
     1981 ctttgaaaag ggtttcggtt accttttggg ccccgccttc cttagagatc actttaacaa
     2041 tatgtcctgg tcttcctctc ttcatcattg catttactac agagacgtca agagcctctt
     2101 ctagtaagag taatttatca aataggttat caaacgcttc agggctcata tcgtcaatat
     2161 tgctttcgat gacaactact gtgtctttct ccatcccttc tgaattaatg ttatcttcac
     2221 caagtattgc tcttaataca tttggatatt catgtgaatt tcttgtcccg gcgccgtaac
     2281 cgatttttga aatcctcatt cttgggaacg aaccgaatcc ctttgagata acttttagaa
     2341 ttgcagcgcc ggttggggtc acgagttcct cgtttctctc tgattcatac atctcgacac
     2401 cacgtagaag ttccaaaact gcgggggatg gacctggata aagcccgtgt tccattttta
     2461 ttaggctcct tgaaaaggga atcgctgaag agtagatctc ctctattcca aggagattaa
     2521 tcccaattgt tgtgcctatt acatccacta ttgaatctat acctccaact tcatgaaaat
     2581 ggatctcttc aggttttaca ccgtgaatct tagcttcggc ctctgccaac ttctcgaata
     2641 ttaaaactgc agtctttctt actttatcgt taagtttact tctttggatt atacttctta
     2701 tctctttaaa gtttctttca gtcttatctt ttccaggtgt tacattaaat ttgtatgctt
     2761 tgattcctga tctatttacg aatctagcgc ttatcttgta tccctttact ggaaggttac
     2821 ttagttcact tcttaggttt tctaggtcta ccccaagatg aataagcgag ccgagaatca
     2881 tatctccgct agcacctgaa aagcagtcaa agtagagcct tttcaagttt gtttcctctt
     2941 attcaataca ttccaaggaa atcatgcctt ccaggagcga ggtagccgat tacaaagaat
     3001 gtaaagagta caactccaaa tccgatcaca gccccaagag cggcttttct ccctctccag
     3061 cctgtggaaa gccttccgtg aattaaaaca gcgtatagaa accacgttac aacagaccat
     3121 gtttccttcg gtccccaact ccaaagtgtt ccccaaattt cttttactga tagtagtccg
     3181 agtgctagtc ctagtgtaag taagggaaaa cccacgaaaa ggcaaacttg gttgagacta
     3241 tcaagcgatg tgagggatgg taatttcatt aaaaaggttc ctacgcgctt cgattttatc
     3301 cgtttttctt ggaaaaggta tagaacacca gcaataaaag ctattgtaaa gagggcttct
     3361 cctaagaaca cgagaccaat gtgagtcaga atccaagggt ttctgaggga tggatcatgt
     3421 tcgattatac cttgggggag aattacagaa ggtatcgtca tgagaaatgc aaaaggggct
     3481 acaaaggctc cgaatactgt ggctttaaac cttgcctgtg aaactatata gactatggca
     3541 ataaaccacg agaaggaata aagactccta tcaagtccac cagcaatagg atcgccttgt
     3601 aaatacgtta agacaagatc tacagtgtgt aacgctactc ctatcctaaa tgtgtgaaac
     3661 ccccatctgg agacactctg gttctgtgtc cagatgtggc tgcagtaaag gaaagaagaa
     3721 atcaaataaa aaagtgaaat aatcaggtga aagtccatgt ttatatttta attaactatg
     3781 gggtacaaat aaataagggt ggatcgtggt ttgtaagtcg tggttcggtg tttgcgatcc
     3841 gtgactcatt ttctcgatgc ccgattcacg atgcacgata cccgaattac gatccgacgg
     3901 cttgttttag tttttcagtt tgataatgtg agattaaagc ttggattagc tcctctaaat
     3961 cgccgtctaa ggcagcatca agcctgtaaa gcgtaaggcc tattcggtga tctgtaatcc
     4021 tattctggga gaagttatag gttctaatct tctcgcttct atcaccgctg ccgaccatac
     4081 ttcgtctcat gtttgatatt tcactttcac gttttttttc ttcgatctcg tagagccttg
     4141 cacgaagaac cttcattgca acaacacggt tctgatgttg agaacgctca ttctggcaac
     4201 tagctactat tccggtaggg agatgggtta tccttactgc cgagtcggtt ttattcacat
     4261 gctggccgcc cttaccacct gcgcgaaacg tgtcaatcct taaatctttt tcatccacag
     4321 taacctcaac atcgtcaggt tcggcaagaa ccgctacagt tgccgtggat gtatgaaccc
     4381 ttccaccagc ttcagtcgtg ggtaccctct gtaccctatg aacaccaccc tcatatttta
     4441 atttgctcca gacgtttttc ccttgtatct cagcaattac ctctttgaat cctccgatcc
     4501 cggtttcgtt catgtccatt atctcgacct tccatccatt cctttcagca tatctcgtat
     4561 acattctgaa taggtctgct gcaaaaagag cggcttcctc accgccggtt cctgcacgga
     4621 tctctataaa tacattctta gtatcatcgg ggtctttcgg gagaagcaaa aggcgaagct
     4681 tttcttcaag tataacctgc tttttttcaa gtgaagaaat ctcttcacga gcaagttctc
     4741 ctacctcttt gtccccgagt agtactctat ttttctcaat ctcttctcga gtacttttat
     4801 gttctctgta agtctcaacg atttcgctta tctcggagcg ttgctttgag tagcggtgga
     4861 tttcagaagg gcttatacta gggttactga ggactttctc aatatcctca tacctttttt
     4921 caaactcttc aagacgtcta agcattccaa tttcagccat attcatttat aatttttaca
     4981 tgccttgcaa aaatttcaag attagattaa actttatgta ctaagattat ggaaacaata
     5041 gatattcttg aaagcaggat acaagagaca ctttcaagga aggcaaggct agaagaagaa
     5101 cgtagaaatc tcgtggatga ggtaatgtta ttacatgaaa acataagaag gcttgagacc
     5161 gaaaaggaag agattaaggc caagttggag gccgttattg agaagataga gctttacctt
     5221 agtaggtcag aagcataaat atgaggcgtg taaagattaa tgtcctaaat aacgagttat
     5281 tcataaaaac agatgcggag gaggaatacg tgcaggagat atcgaattac ctagaagata
     5341 aggtaaggga agcatctccg caagtagatg cggtggttgt gcctcgtcct tttcttcttg
     5401 caacgcttaa gattgttgat gattatttca ggttaaaaag agaatacgag gagtttaaaa
     5461 atcgtgctga ggaaagatca agaagactgg ttgagatatt ggatagttcc cttggggaaa
     5521 aagagacctt tgaatattgg aggggaggaa ggagagaaaa ccagaaaaag gaggtttaga
     5581 atcaacctaa ctagtgggtt tgaagaacct gctttaaatc tctccacttt acagaggaag
     5641 cgttttcacg ttattgaaga aaaaggtgat ttacgtctcc gtttactcga gaaaaggcta
     5701 agtcttaatc ccaaagatgt atggataaga agcgaagata ttaaacgaag acttatcaag
     5761 cttaaatctt ttcaagaagc ccgcagcatt gccctttact atccgataag aaatgaagtt
     5821 ggcactcagg gtatattcaa gattgccaaa gaactaggga aaggagtcca cttcccacta
     5881 gtttatggat cttcactaga atttctaaaa ataaatgacc tagctgagct aaagatcgga
     5941 aggtttggcg ttcctgaacc aagccatttt tccccaaggg ttgaaattga aaatatagat
     6001 ctaattgtca tacctggtat tgcatttgat aggtttgggg caagaatagg ttacggcaaa
     6061 ggatattttg atcgtacgat atctaagatt gatataaaaa agagggtagg gttagcttac
     6121 gatcttcaag tagtagaatc agtaccgctt gaaagatacg atgagagggt aggtttgata
     6181 gttactgaga cgggtgcaat tatatgttaa aggaggatta gattgtgatg atgtggttta
     6241 taatagcgac tacactggct tttatactag gagctttagg gtattacctt tttcgtgcga
     6301 taagggagaa gagaaacctt gagttaaaaa gaaccgaagc gcaaattgtt atcgaggcag
     6361 ctaagaaaga ggctcaaaag attagggccg agggggaact tcaggctaaa gagattattg
     6421 agaaaagcaa atcagaggca gatcgtgatt atcgtgagaa acaaaaagag gtatctaaga
     6481 tagagaaaag acttcagagt cgtgaagaga acatagataa aaagtacgaa tccatagaaa
     6541 gaaaggagac cgagatttca cgaagggaaa gggagacttt atcaaaggag aaggtcgtcg
     6601 cagaaaagga aaaggaactc aactcaatca tcactgaggc aaagaaaaag attgaggaag
     6661 ttgctggtat gagtcaagag gctgctaaaa gagaactcat aaatattatc gagaacgagg
     6721 ctaaacatga ggctgcaaaa aagcttaaac tgattgagga cgagcttcat gaaaacgctg
     6781 agagtaaatc aaaagatatt atagctcttg ccattcagaa gtacgcagga caatacacaa
     6841 gcgaaaaaac gatatccgta ttgaacctcc ctaatgatga aatgaaggga agaatcattg
     6901 ggcgtgaagg aagaaacata cgttcaatcg aggctagaac aggcgtagac atcataattg
     6961 acgacacccc tgaaacagta gtcgtctctt ctttaaatcc aataagaaga gaggtagcta
     7021 gaatagcttt agagaggcta attgcagacg gtaggattca tccagcaagg atagaggagg
     7081 ttgtaactga agtcgaggat gagattgaaa aggagatcca gaaagctggt gaggaagccg
     7141 tttttgatat tggagcacac aacatgcacc ctgagcttgt gaagcttgta ggtaaattaa
     7201 aatacagaac aagcttttct cagaacatac tcaaccactc acttgaagtg gcttttatct
     7261 gtggtgtaat cgcgggcgaa ctcggtctca atgtgaagac tgcaagaaga gtaggtcttc
     7321 tccatgacat tggcaaggcc ctcgaccatg aggtagaagg ctctcacgtc gaaataggtg
     7381 aagaggtggt taggaaatac ggggaaccgg ctgatgttgt agaagctgta gcaaagtacc
     7441 atgacccaca gcctcaatct atttgggcta tacttgttca ggcttctgat gctatttcag
     7501 cagcaagacc cggtgcaaga agggagatgt atgaaaccta cgtgaaaaga atcgaggaca
     7561 ttgagaggat tgctagctct tttcgtggtg tagagaaatg ctatgctatt caagccggaa
     7621 gggaggtaag ggttattgtt gagagtggta aggtgaatga cgaagaagcg gtactgcttt
     7681 caaacgagat tgctaagaag atccagcgag aggttgcctt tccgggtcag gtgaagatta
     7741 ctgtgataag agaagtcagg gctacagctt atgcgacgta gataatcaag tgcctgattg
     7801 gttaattggt tgatgattga gcaagctagg ttacagggcc ttttaaaatt taatcaggta
     7861 ataaccgaag ggtagcccat cagtcttgct gatgggccag atggcgacga gcaagactcg
     7921 ttgcctaccc agttagatta tctatgagcc gtttaaatta aaatacatat gtgatctact
     7981 ctcagctata atttcatttt ttgaattatg gagaaaaagg aaggattatg tgttctgttt
     8041 atcggggaca ttgttggtga gcctggaaga aatgcagtaa agaccctact cccaggtttg
     8101 gttaaagata atcttgttga ctttgtcgta gcaaactgtg aaaacgccgc ggctgggaag
     8161 gggattactc ctaaaatcac ggagtattta ctgaacacag ggattaatgt tcttacatct
     8221 ggaaaccaca tctgggatag gcaggagatc attccataca tagaaactca gccgctactt
     8281 ctacgccctg ccaattatcc aaaaggaacc ccaggaaaag gacacggtat ttttgaaact
     8341 aaaacgggct atagaatcgg tgtggttaat ctttgcggca agctttttat ggattcttat
     8401 gaacatccat ttcttactgc tttagattta gtaaacgagt tatcaaaaca cactcgcatg
     8461 ataattgttg actttcacgc cgaggcaaca tcagagaaga aagctatggg ctggtactta
     8521 gatggtaagg tgagcgctat aattggtact cacacccacg ttcaaacagc agatgaaagg
     8581 gttcttccaa aaggaagtag ttacataaca gatgttggaa tgactggatc aatcgattcg
     8641 gtaattggaa tggataaaga aaaggctata aaaaggttcc ttacgctaat tcctataaga
     8701 cttgagcctg caactgataa tgtttgtctc aatggggtat tagttgattt agataaagac
     8761 agtggtaaaa gtttagggat caagagaata gtcgttaatg ccaaataatc attaatgaat
     8821 caattaaggt atctttactt ttaactgtgt cggggtcata aaaccctcct cactctcctt
     8881 caaaagtttt tattagtatt aaacgtccat tttcaaatct attcgatttc gagcttctta
     8941 cccctagttt ctggaagggt taaaacaact atcgcagcgg ctactgcaaa cacagaaaca
     9001 gtgataagtg aaagcccata accgtagatt cccgcaacat atccgactaa aggaggagct
     9061 acagctgaaa cccctcttcc acagttataa cagaaaccct gcgctgtgcc tcttgcacgc
     9121 gtagggaata tctcagagaa aatagcgcca aatcctgagt aaaatcccgt gccgaagaag
     9181 ccgataaaag gaccaagaat aagaacagtg gtcaaatcct ttgcatgacc gaagaaccag
     9241 actagtactg ccataattat caggtaagca acaaaaacgg gtctccttcc aaatcggtcg
     9301 cttatccaac caaatgtgtt acacccaata aaagctccaa tatttatagg aataatccaa
     9361 gcaaagccct ttggacctaa tcctatccct ccttcagcaa ccggtcttga aagaaactgt
     9421 ggtatccaga agtaaagacc ccagtaggcg atcatacata ggcttgtaag aagggtggcg
     9481 gtaatagtat atctgattaa atcccgcctg aagatctgat ttagggtaaa actctgccct
     9541 ttctcgctga gttttccttc ttttctcatg tcagccgttt ttttccaaat ttctggttca
     9601 tctaggtgcc ttctaatgta gaaaactaaa aaagcaggtg taatacctac aaggaaaagg
     9661 attcgccagc ctaggttcac gtcaaaataa gggatgatta aggtagccag aactgcggcc
     9721 aaaatatagc ctattcccca gccactttga accatcccga tgaccttgcc tctatgctgc
     9781 ctaggccaac tctcggctac aaggacctcc cctgatgcca attctcctcc catacccaga
     9841 ccgagaaaca aacggcaaac caggagaaag gtcacattag gtgagagggc actaagtcct
     9901 gtaaaagcgg aatataatag tatggtataa gttaaagcac ggacccttcc tatgtaatcg
     9961 gatacgattc caaatactgc tccgccaaaa gcggatgaga aaagcgttac tgaaaaaatg
    10021 gctcctacct gggagggatt gagcttaaat acggtggcga tgctactaac ggcaaatgca
    10081 aataaaagaa ggtccatagc atcaagtgtc caaccaagaa atgctgcaat gaaagccttc
    10141 cactgctgtg catttatatc tttataccaa ccaaaaactc ccagcttaga aacagaaacc
    10201 tctatcgttt cattactcat tgcttagccc cctttcaatg tttagtttag cagttactac
    10261 caaactatgg gtggaacttt ttattatggt ctagcctaat aaatttcatt tgatctgtca
    10321 atctttttgt taaaggtttg aattttgcgt catatattag attttactta ataaaattta
    10381 acgatttcca ctgaatccac gcttgatata ttaactacat ttaagggatt tcatcaactg
    10441 gtttaaatga agcgaattaa acaaatacaa aaaggatata aaggacttta actactattc
    10501 agaaatggat gtcggcacat tagcttgact tcaatatgtt aaggcgttac agtttaatcg
    10561 aaagatgcag aggaaatact tatatttaga aacatacggt tgtcagatga atgaatacga
    10621 ttcggataaa atccggagtc tccttggaat ggagcttaca aacgatccga aaaaggcgga
    10681 cgtcgtaata attaacacct gtgcgataag agaaaaggct gatcaaaagg catttagcag
    10741 tttgggtagg ttaaaacacc taaaatttag aaaacctaaa atgatacttg gcgttggggg
    10801 atgtgtagct caactctatg gagaaaggct tattgaaaat tttcctcatc ttgatcttgt
    10861 atttgggaca agaactataa cactccttcc cgaacttatt tctcaggtag aaagggaaaa
    10921 gagaaggatg gtggaaacat catttgatat agaagaggtt tttgagtttg agccctatca
    10981 tgaagaaggc aaggtaacag cgtttgtctc tgtacaacaa ggttgcaaca aaatgtgtag
    11041 ttattgtatt gttccattag tgaagggaaa agaaacaaac agatctgtcg aggacattct
    11101 agcagagacg caaaaacttg ttcaaaaagg tgtgagggag ataacactga taggacagac
    11161 agtaaattca tggaaaatgg cggggtataa attcggtgac ctactaaaga tcatggcgga
    11221 gatcgaagga ctattccgca ttcgctttac tacatcatat cctagggatg tcacaaagaa
    11281 attgattgat gcgataagag atgttccgaa ggtttgtcat catatccacc ttccagttca
    11341 gtctggctca aataggattt tgaccgaaat gggaagaaca tatactagag aatggtatat
    11401 ggatatacta aagagaatta gggatgctat acctgagatg gcgatcacaa cagacataat
    11461 tgtaggattt ccaggtgaaa cagaggatga ttttaacgat acgatgagtc tcctcaaaca
    11521 agcagaattt gatggtacct tttcctttaa gttttcgcct aggcctggaa cgcctgcttc
    11581 tgagtttagt gacatggtct ctgatcaagt ttccagtaga agacttatgg aattgcaggc
    11641 gtttcaaagc gaaattacgg ttaagaagaa tcaaagcata gttggacata ttgaggaagt
    11701 tcttatagaa ggtgagagta agaatgatcc aagctgggtc acgggtagga cttctcagaa
    11761 cagggttgtt aattttcctg ggacgactga cttaaggggt aaactcgaaa gggtaataat
    11821 aacagagggg tttcaaaact cccttcgtgg gagattatta gaaaatgaaa tggaggtgag
    11881 ccatgtttct tgaaatgaag gtttcgggaa ttgcattaga cccttttaca aatactccta
    11941 tagttattct aaaagacctg actggtgaga gggtgctccc aatatggatt ggttttatgg
    12001 aggcaagcgc catagcaatg gagcttgaaa aaacccctag ggctcgtcct cttacacatg
    12061 atttgatgaa agccctatta gaaaccctga aattctcagt aataagagtg gatgttactg
    12121 atctaagaaa tgatacattt tacgcagaga tccgcctaca aagaggaagt gaggaacata
    12181 tcgttgattc aagaccgagt gatgcaatag caattgcatt aagaactggt tccccaattt
    12241 ttgttaggga tgaggtgctt gagaagtcaa agaaactcga gttcgacgag gaaaaggata
    12301 aacttagtga tctattagaa aagatccccc ccggagattt tggaaagtat aagatgtaaa
    12361 gctgctgaag cttcatctgt acgttattaa atccatcttg gtgtgcgatt aatttttttt
    12421 atatagattt atctattact cgctatgttc gaattttaga tgcggtaact acctttcctt
    12481 acttaaaaac cttttgttta ttaagttgtg gatctaatac tggattgccc tttaaaagca
    12541 attatgagaa agtggaatta catattttct ttatcatcgg ccccctcttc ttcctcttct
    12601 aagagaccat gcttagcaag tctatatcta agtgaacgaa aggtaatccc aagaagcttt
    12661 gcagcttcga ctttacttcc ctgagcctgt gcaagtgctt tttctatcgt tctcttttca
    12721 aacgtttcaa gaaggtggtc caagccaaag ttacctgcga tcccgttttc aatattgagg
    12781 gagtccatgt taaccataag ctctggaagc gtttgttgac gtatcacatt agatgtctca
    12841 agcgcgacgc accttgcaat tatattttca agctctctta catttccagg ataatgatag
    12901 ctctcaaggg tttctaatgc cttag
//
LOCUS       DATDRL010000185         3370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2246304, whole genome shotgun sequence.
ACCESSION   DATDRL010000185 DATDRL010000000
VERSION     DATDRL010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2246304"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VI935_06395"
     CDS             complement(<1..952)
                     /locus_tag="VI935_06395"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HLE25265.1"
                     /translation="MALIVQKYGGTSLANLKKIRQVAEHIIKTRKKGNDVLVVASAMA
                     GETDRLLKLSRSIMDPPDERELDVVTSSGEQVSSGLLAMMIKSLGYDAVSFQGHQVRI
                     ITDNAYSKARIVKIDDEKIKRALLNGKIVVVAGFQGVDEEGNVTTLGRGGSDLTAVSL
                     AAVLKADACELYKDDVDGVYTTDPGICRNARKLKKISYDEMLEMASLGSKVLQARAVE
                     FAKKFSVPIHVRPTGRPDTEGTWVVEEEAAMAEMDDIVISGVTYDKNQAKITIMQVPD
                     RPGIAAKLFTPIGNEGIVVDMIVQNVSTEGYTDMTFTVPRT"
     gene            complement(904..1398)
                     /gene="tsaE"
                     /locus_tag="VI935_06400"
     CDS             complement(904..1398)
                     /gene="tsaE"
                     /locus_tag="VI935_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HLE25266.1"
                     /translation="MHRASCNILVSKEVATIKVGEVLGRSLKTSDIVALKGELGAGKT
                     VLVKGIAKGLDVKEEPNSPTFVIMNAYEGRIPLYHFDFYRVSGVYELEGIGYDDYFYG
                     NGVTVVEWADRVEGVFPESAIWIEIKIPAKKNTNSNVREINIEGEKKWLSLFRSMVER
                     VLPI"
     gene            complement(1483..3039)
                     /locus_tag="VI935_06405"
     CDS             complement(1483..3039)
                     /locus_tag="VI935_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HLE25267.1"
                     /translation="MKLATREIIRKIDRISIEEYGISGLILMENAGRAVAEVILDEFP
                     KVKRVAIFAGGGNNGGDGFVVGRHLTNKGLDVTTYLTVDPKIYKGDALTNSQALSKIG
                     GKVIRLNNGFRDYKGADLIVDALFGTGLDREVDGFYREVIDFINSQPVPRVAVDIPSG
                     LDSDTGFPLGVSVKADVTVTFVLPKIGLAIYPGIDYAGKVYVVDITTPRFLEKDIPFE
                     LITYDTVREILKPRHPNSHKGTYGHLFILAGSPGKTGAATLAALGGLRVGTGLVTVGI
                     PKSLNPIMEEKITEAMTEPLPETENTTLGKVSIEASKRIISARKTALAIGPGISTTAE
                     TGEFFFEILRSSSLPMVVDADGITILAQNLKILKKLKVPLVLTPHPGEMSRLIGKTGE
                     EVQRSRISVARDFSSMYNVYTVLKGARTVISTPDGKVFINPTGNPGMASGGIGDVLTG
                     VIGGFLAQGYNPIDACILGVFAHGLAGDIVACKRGVAGIIASDVAEALPETLNEIING
                     EKEDFFYRIS"
BASE COUNT          928 a          862 c          569 g         1011 t
ORIGIN      
        1 ccgttcttgg aaccgtaaac gtcatatccg tatagccttc tgtgcttaca ttctgcacga
       61 tcatatcaac aacgattcct tcattgccta ttggggtaaa aagctttgct gcaatcccgg
      121 gtcggtctgg cacctgcata attgtaatct tcgcttgatt cttatcgtat gtcactccag
      181 atataacaat atcatccatt tctgccatag ctgcctcctc ttctacaacc catgtcccct
      241 ccgtatctgg tctcccagta ggacgcacat ggattggaac actaaatttc tttgcgaatt
      301 caaccgctct tgcctgaagc acctttgaac caagactcgc catctcaagc atctcatcgt
      361 aagagatttt tttgagtttt ctagcattcc tgcatatccc tggatcagtc gtgtaaactc
      421 catctacatc atccttatag agttcacatg catcggcctt cagcacggcc gcaagagaaa
      481 cagcagtgag atcagaaccg ccacgaccaa gagtagtaac gtttccctct tcatcaaccc
      541 cctggaatcc ggctacaaca acgatctttc cgtttaaaag cgcccttttt attttttcat
      601 cgtctatctt gactatcctt gctttggagt aggcgttgtc agtaataatc ctcacctgat
      661 gaccttgaaa tgagactgca tcgtagccta aggatttgat catcatagca agaagccctg
      721 aagatacctg ttcaccgctt gatgtgacta cgtctagctc cctttcatca ggaggatcca
      781 tgatcgatcg agaaagcttt aaaagacggt ctgtttctcc agccatagca gaggcgacta
      841 ccaaaacatc gtttcctttc tttctagtct taattatatg ctctgcaacc tgtcttatct
      901 tttttaaatt ggcaagactc gttccaccat acttctgaac aatgagagcc actttttctc
      961 tccttcaata ttaatctcac gtacattgga attagtgttc tttttcgctg gaattttgat
     1021 ctctatccat atcgcgcttt caggaaaaac tccctccact cggtcagccc attcaacaac
     1081 cgtaacccca ttcccgtaga aataatcatc gtatccaatt ccttcaagct cataaacgcc
     1141 agaaacccta tagaagtcga aatgataaag aggaatcctt ccttcgtaag cgttcataat
     1201 gacaaatgtg ggactattag gttcctcttt tacatccaat ccttttgcaa taccttttac
     1261 tagaaccgtt ttcccagccc ctagctcacc tttaagagca acaatatcac tagttttaag
     1321 cgaccttcca agcacctctc caaccttaat tgtagccact tcttttgata caagaatgtt
     1381 acatgatgca cgatgcatga ttcatgatac atgattaatc ttttatcctg catcttgcat
     1441 cctgaatcct gtatcgggta ccatcctgca tcctgcctat aactaactta tcctatagaa
     1501 aaaatcctcc ttctccccat taataatttc gttgagtgtc tctggtaaag cttcggcaac
     1561 gtcgcttgca attattccag ctactcccct tttacatgca acaatatctc cggctaatcc
     1621 atgagcaaaa acccccaaaa tgcacgcatc gataggatta tatccttgcg ctaaaaaacc
     1681 acctataaca ccagtgagca catccccgat tccacctgaa gccattccag gattacctgt
     1741 aggatttata aataccttgc catcaggagt cgaaatcacc gttcttgcac ccttaagcac
     1801 agtatagaca ttatacatag atgaaaaatc cctagcaacg ctgatgcggc ttctctgaac
     1861 ctcctctcct gtttttccaa ttaagcgaga catctctccg ggatgggggg tgagaacaag
     1921 aggaaccttg agtttcttta agatcttcaa gttttgagca aggattgtaa taccatccgc
     1981 atcaactacc attggtaaag acgagcttct aagaatttcg aagaaaaact ctccagtttc
     2041 tgcagttgta gaaatacctg gcccaattgc aagtgctgtt tttctagctg aaattatcct
     2101 ttttgacgct tcgattgata ccttccctag agtagtattt tcagtttcag gaagaggctc
     2161 tgtcattgct tcagttatct tttcttccat gatcggattt aagcttttag ggataccaac
     2221 tgtaacaagc cctgtcccta cccttagacc gccaagtgct gcaagtgtgg cagcaccggt
     2281 ttttcctgga gaaccggcaa gaataaaaag atggccgtat gtccctttat gcgagtttgg
     2341 atggcgtggt tttaggattt ctctaacagt atcatatgtt ataagctcaa acggaatatc
     2401 cttttcaagg aacctagggg tagtaatatc caccacatag accttgccgg cgtagtcaat
     2461 tcctggatat atggcaagtc cgatttttgg aaggacaaac gttacagtaa catcagcctt
     2521 tactgaaact cctagtggaa atcctgtgtc ggaatcaagt cccgaaggga tgtctacagc
     2581 cacacgaggc acaggctgag aatttataaa atcaattacc tccctgtaaa aaccatcaac
     2641 ctccctatcc agccctgttc caaaaagtgc atctacaatc aggtctgcac ctttataatc
     2701 tctgaatccg ttattaagtc ttattacttt ccccccgatc ttactaaggg cttgagaatt
     2761 agtaagcgca tcccctttat atattttagg gtccaccgtt agatatgtgg ttacatctaa
     2821 gcctttattc gtaagatgtc ttcctacaac aaatccatct cccccattat ttccgccccc
     2881 agcaaagatt gcaacccttt ttaccttggg aaattcgtca aggatgacct ctgccacagc
     2941 acgaccggcg ttttccataa gaattagccc ggatattcca tactcctcga ttgaaattct
     3001 atctatcttc cttatgatct ctctggttgc tagtttcatg tctgaggatt aacacctaat
     3061 aattattgag aaataaatat atacaataaa ctaccttttg ataaatcgca taagctccct
     3121 gagctttgca gaacgctagg catttaggta aaaaagtgaa tgaacaaggc tatccgcaat
     3181 gtggaggaaa aagttctcag gagcttttac aatagcagct ctccgattgt aaatatcgag
     3241 aaaaactcat accccatatc ttcaagtgtt ctcctggctc cttcttctct atcaactatc
     3301 accaaaactt ttttcacaat agcaccagca ccctttgcag catcaattgc ttttagaatt
     3361 gacccacccg
//
LOCUS       DATDRL010000186         7795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2397713, whole genome shotgun sequence.
ACCESSION   DATDRL010000186 DATDRL010000000
VERSION     DATDRL010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2397713"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            512..958
                     /locus_tag="VI935_06410"
     CDS             512..958
                     /locus_tag="VI935_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HLE25268.1"
                     /translation="MWIFKTYPSWAPIFARLTLGIIFFAHGSQKLLGWFGGPGWSGAI
                     QFFEQNLSVPPFLTGLLIITEFFGGIAIILGFFTRLAALGLTIAMLVAIYKVHLQYGF
                     FLNWFNLPGVGHGIEMSLALIGLSLSLLVWGAGNLSVDQMIAGEKK"
     gene            1206..1949
                     /locus_tag="VI935_06415"
     CDS             1206..1949
                     /locus_tag="VI935_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25269.1"
                     /translation="MGTKREKIVSKAVEIIKSFQDGIRYSELVRRLQKELPDIPTNTI
                     HGTIWNLEVLLPNEIYKPARGLFRHTAFSDAQVSEVERRVPTRIEKVREEHFYQPFAD
                     WLVKELEECTKAISLGGNKFKDKWGTPDVIGIREPRKSDIIKPPTEIVSAEIKTDTRD
                     LITAFGQACSYKLFCHKSYIVVPKESPEEDITRIDALCLIFGIGLILFDSNSSKAPQF
                     EIRVRPSKHEPDMFYVNRYMKLIEDELFS"
     gene            2147..3844
                     /locus_tag="VI935_06420"
     CDS             2147..3844
                     /locus_tag="VI935_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25270.1"
                     /translation="MKNLICVIMTINRLLTKNLLSSLIVVWVLGPSAFAQTAGHNHPF
                     TDTKPAVLMSGLGEQHHPVSTKNPEAQRFFDQGLTYIFAFNHDEAVRSFKRTTELDPQ
                     LAMAYWGIALALGPNINLDVDPEREKAAYEAVQKALTLSANAPEQERAYIESLAKRYS
                     IDPKADLKKLAVDYKNAMEELVKRYPDDLDAATLYAESMMDLRPWQFWNKDGTPAEGT
                     EEIVTVLESVLKRNPNHIGANHYYIHTVEASLHPERALPSAERLKTLAPAAGHLVHMP
                     AHIYMRTGDYKSAAQRNKVAAEVDQAYIKSSGVQGVYPLMYYSHNLHFLAVAYAMEGR
                     FSDAKKAADQLEAHVGPHVKGMPMLEFFMPTSTLILVRFQRWGDILESPKPNPNMSIT
                     TAVWHFVRGMAYAATGKVKEAESERKQFISVMKTIPAGKTWDLNTVNSVLEVAENVVD
                     ARIALAKGDNKSAIELLKKAVEAEDALNYAEPPGWYIPTRESLGSVLMLNGEYAEAEK
                     VFRVDLDKNPRSGRSLFGLLKSLKAQGKNYDAQLVQKEFEVAWKNADIQLKQVEDLL"
     gene            complement(3940..5100)
                     /locus_tag="VI935_06425"
     CDS             complement(3940..5100)
                     /locus_tag="VI935_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HLE25271.1"
                     /translation="MLPLQGLKVLDLTRLIPGGYATLILTDLGAEVLKIEDIELGDYS
                     RWMEPTIGEYGVYFHALNRNKKSMKLNLKAEDGKRIFKKLVEKGYDTIVESFRPGVMD
                     RLGIGYNPLREINPRVIYCAISGFGQNGPKKEKALHDLNCLAVSGALSITGEEAPSIP
                     GIQIADTSSALFSVISILTAYIARGKTGQGQFIDVSMTDGVISLLSIHLIKFFVDSKI
                     PEGGRMQFDGQFPYYRVYKTKDGKYMTLAALEKKFWEKFCTAIGRKDLIDNQYDDSIE
                     TRNEIENIFKSKTRIEWEEISENIDSCLEPVLNFKEVTEHLQLKSRNLFFYIDHDNVK
                     VPQVRIPFLMSDVKNTPRNPAPLWGEHTEEILISIGYEKEEIASLKNKGVVG"
     gene            complement(5176..6153)
                     /locus_tag="VI935_06430"
     CDS             complement(5176..6153)
                     /locus_tag="VI935_06430"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HLE25272.1"
                     /translation="MIPVRILGTGSSAPERILTNFDFEKIVDTSDEWIRTRTGIVERR
                     IADPDVATSDMAYEASLRALEAASVDPIDVDGIILGTVTPDYFFPSTACLLQSRIRAK
                     RAFAFDLSAGCSGFVYGLQLGKAMIESGEAKIILIVGAETMTRIMDFNDRATCVLFGD
                     GAGAALIGKSDMSGILSLCLGANGDDWELLHMPAGGSRIPPSEESIRNRLHYIKMKGN
                     DIFKEAVKAMESASIEAIRKADIAPDDIDLFIPHQANLRIMEAVRKRLDIPPEKVFIN
                     VDRYGNTSSASVPIALDEAVRSGRADRGDLILFSVFGAGFTWASGVIRW"
     gene            complement(6218..6604)
                     /locus_tag="VI935_06435"
     CDS             complement(6218..6604)
                     /locus_tag="VI935_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25273.1"
                     /translation="MTLSFMESGCLATSLNDMTVFVFKGSHNEIESINNTSQIFFGLE
                     CIKRPEFPSVRMYFDLRDKSKKPFLFDYFFNIESDEDMKLLGKLAEQDYFDILLFDSI
                     ISYSKRVEITKREKEKIKEVLDQARA"
     gene            complement(6986..>7795)
                     /locus_tag="VI935_06440"
     CDS             complement(6986..>7795)
                     /locus_tag="VI935_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HLE25274.1"
                     /translation="GGTLAIAGASLQALFRNPLAAPFTLGISGGASLGAIIAIRLGLG
                     NTFLGFSSISIVSFIFSIFTILFVYSVSKVHGVVATGRLLLAGVVVNFLYSALILFIQ
                     FFSDFIQSLQTIRWIMGSLDIVGLDTVWKTLIFAIPGCVILLSITKDMNLFALGDDVA
                     SSLGVNIGLMEKKIYFATSLAVSAVISVTGPIGFVGLIIPHVLRMIIGVDNRIILPSS
                     FLLGASFLTAADTVSRTIFAPIEIPVGIITASCGGVFFLWLLMRMKKEVIV"
BASE COUNT         2286 a         1591 c         1634 g         2284 t
ORIGIN      
        1 ctatagttac ggagaccatg taaaggattc ctacgttaaa tccagcatct ccaaatgcaa
       61 agagcactag aggaagcccc atgttgcccg tattagcaaa aagaattggt ggtagataca
      121 cttttatagg taggttgagt gatttgatta tcaaatagcc tataacaaga gacccaccaa
      181 taattaagag tactgagaga aatacctttc cagcaaggaa aatttcaatg ggttctttag
      241 ctagtgaatg taatattaaa cacggggttg atatgtatat aacgaaatcg tttattgcag
      301 taagatttat ttttcttatt ttgccaaata gataacctat gaaagttata agaaataccg
      361 gaagaacaat atttgcaatt tcaataatga tgctagtatt ccccctttaa gggttggtca
      421 tccattttac ctaatttaaa tgggattgag atttcttaat gaaatatata ttatgatatg
      481 tgtttgaaat aaccttaaag gagggataac catgtggata tttaaaactt atccttcatg
      541 ggcgcctatt tttgcccgtc ttactcttgg cattatattc tttgcccacg gctcgcaaaa
      601 gcttcttgga tggtttggtg gtcctggttg gtcaggagct attcaatttt ttgaacagaa
      661 tctaagtgtt ccgccttttc ttacaggtct tcttataata actgagttct ttggaggaat
      721 agcaataatt ttgggttttt ttacacgcct tgcagctttg gggcttacaa tcgcgatgct
      781 tgttgcaatt tataaggttc atcttcaata cggattcttt ttaaactggt tcaaccttcc
      841 tggcgtagga cacggaatag aaatgagcct tgctcttatc ggactttcac tttcgcttct
      901 tgtttggggt gcagggaatc tgtcagtcga tcagatgatt gctggggaaa agaaataggt
      961 cgagtcatct gtcttagtga gttaacaata aactagcctt tgtttcggtg tttaagagtt
     1021 tagttctgct gtccttttta aatcgggtgg agggtaaact caaggttaaa aaataattgg
     1081 gaagacaaaa tgggttttga tatgctgaaa aatatctata acactgagga cattgcgcaa
     1141 attttgtagg tataagcgca ttaatgcgtt tttattatag gtaaccctaa gaagaacgag
     1201 acaatatggg tactaaaagg gaaaagatcg tttctaaggc tgtcgaaata ataaaaagct
     1261 ttcaagatgg tattcgctat tcggaacttg taagaagatt acaaaaagag cttccggata
     1321 ttccaactaa caccatccac ggaacgatct ggaatttaga agtgctcttg cctaatgaaa
     1381 tctacaaacc agcacgtgga ttattccgtc acaccgcatt cagcgatgct caagttagtg
     1441 aagtcgaacg cagggtccct acgcggattg agaaagtcag agaagagcat ttttatcaac
     1501 cttttgcgga ttggcttgtc aaagaattgg aagagtgtac aaaagcaatt tccttgggag
     1561 ggaataagtt taaggataag tggggaacgc ccgatgtaat cggaattcgc gaaccccgca
     1621 aaagcgacat cattaagcct cctactgaga ttgtatcggc agaaattaaa acagacacga
     1681 gggatttaat cacggcattt ggccaagcct gctcctataa gctcttctgt cataagtcgt
     1741 acatagtagt tccaaaagaa tctccagagg aagatattac tagaatcgat gccttatgct
     1801 taattttcgg gattggtcta attctgtttg atagtaacag ctctaaggcc cctcaatttg
     1861 agatccgggt tagaccaagc aaacacgaac ccgacatgtt ctatgtgaat agatatatga
     1921 aattgattga ggatgagtta ttttcttaag actttgccga atatagttac attattaccc
     1981 tgatagcgca tttgtggctt gctctgcttg tccataattg ccttagataa tttgcaaata
     2041 ctcaagacgt taaactgggt tggtgtttgt gaaaattttg cttacactgt tcttcataga
     2101 agctgctatc gtcacttaat taacaaaata caaataaaaa ttaataatga aaaacttaat
     2161 ctgtgttatt atgaccatca ataggctcct aacgaaaaat ttactcagct cattaatagt
     2221 ggtttgggta ttgggcccat cggcctttgc gcaaactgcc ggacataatc atccgttcac
     2281 cgatactaaa ccggctgtgt tgatgtctgg cttgggagag cagcatcacc cagtttcgac
     2341 gaagaacccc gaggctcaac gtttctttga tcaggggctt acttatattt tcgcctttaa
     2401 tcacgatgag gcggtacgct cgtttaaacg cacgactgaa cttgatcctc agttggctat
     2461 ggcgtattgg gggattgccc tagcgcttgg acctaacatt aaccttgatg ttgatcctga
     2521 acgggagaag gcggcatacg aagctgtcca gaaggctttg acattatcgg caaatgcacc
     2581 ggaacaagaa cgtgcctata tcgaatcact ggcgaaaaga tactcgattg atcctaaagc
     2641 cgatttgaaa aaactcgcag ttgattacaa aaacgcaatg gaagagcttg tcaaacgtta
     2701 tcctgacgat ctcgatgccg ctactctcta cgccgagagc atgatggact tgcgtccatg
     2761 gcaattctgg aataaagatg ggactcccgc tgaaggcaca gaagagattg ttacagtgct
     2821 tgaatcagtt ctcaagcgta accctaatca cattggcgcc aatcactact acatccatac
     2881 cgttgaggcg tcgctacatc cggaaagagc gctgcccagt gcggagaggc ttaaaacact
     2941 ggctccggcg gcaggtcatc tagtccacat gcctgctcat atttatatgc gtactggtga
     3001 ttacaaatct gcagctcaaa ggaataaagt tgctgctgag gtggaccagg catatatcaa
     3061 gagtagtggg gtgcaagggg tatatccatt gatgtattac agccacaatc ttcatttctt
     3121 agcagtggca tatgccatgg aagggcgatt ttcggatgct aagaaagcag cagatcaact
     3181 tgaagctcac gttggacccc acgttaaagg aatgcctatg cttgaattct ttatgcctac
     3241 ctcgactctc attctcgttc gttttcaacg ctggggcgac atcttagaat ccccaaagcc
     3301 caatccaaac atgagcatta caactgcggt gtggcatttt gttcgcggca tggcttatgc
     3361 agctaccggc aaagtgaagg aagctgaaag tgaacgaaag cagtttatct ctgtcatgaa
     3421 aacaatacct gcagggaaaa cttgggatct caataccgta aacagtgttc tggaagtcgc
     3481 agaaaacgtt gttgacgcca gaattgctct ggcaaagggt gataacaaat ctgcaattga
     3541 gcttttaaaa aaagcagttg aagcagaaga tgcgctcaat tacgccgaac ctcctggttg
     3601 gtatatccca actagggaat cactcggtag tgtgcttatg ttgaacggtg agtatgcaga
     3661 agccgaaaag gtttttcgtg tagatcttga caagaatccc cgaagtggac gttcgctttt
     3721 tggactgctg aagagtttaa aagcgcaagg gaaaaattat gatgcgcaat tggtgcaaaa
     3781 agaatttgaa gtcgcctgga aaaatgcgga cattcaactc aaacaagtgg aagatctatt
     3841 atgaaggctg aaggaaggga tggagaacca cgcttacagc gcgagttaga aatgttaata
     3901 attaggctta gaaaagggag agttgatatt ttgaactctt caccctacca ctcccttatt
     3961 tttaagagac gcaatttcct ctttttcata tccaatcgaa attaaaattt cctcggtatg
     4021 ctcaccccag agaggagcag ggtttcttgg tgtgtttttt acatctgaca ttaaaaaagg
     4081 aatcctcacc tgtggaactt ttacgttatc atggtctata tagaaaaaga gatttctgct
     4141 ttttagctgt agatgctctg taacctcttt aaagttaagc acgggctcaa gacaggaatc
     4201 gatattttct gaaatctcct cccattctat tctggttttt gatttgaata tattttcaat
     4261 ctcgttcctg gtttcaatgc tgtcgtcata ctggttatct atcaaatctt ttcttccaat
     4321 tgcagtgcaa aatttctccc agaatttttt ttcaagtgcg gcaagcgtca tgtatttccc
     4381 gtcttttgtt ttataaaccc gatagtatgg aaattgtcca tcaaactgca ttctcccccc
     4441 ttccggaatt ttgctatcaa caaagaattt aatgaggtgt attgaaagaa ggcttataac
     4501 tccatctgtc atagaaacat ctataaactg tccttggccg gtttttcctc gtgctatata
     4561 ggccgtgaga attgaaataa cagaaaaaag tgcactgctt gtatcggcaa tttggatccc
     4621 tggaatcgaa ggagcttctt cacctgttat gctgagagcc cctgatactg ccaggcagtt
     4681 taagtcatga agggcttttt cctttttggg cccattctgc ccaaagccac ttattgcaca
     4741 gtatataact cgtggattta tttctctaag agggttgtaa ccaatgccca atctatccat
     4801 aactccaggc ctgaaactct ctactatcgt gtcataacct ttttctacaa gttttttgaa
     4861 tatcctcttt ccatcttctg cctttagatt tagcttcatg ctttttttat ttctatttaa
     4921 agcatgaaaa tatacgccgt attccccaat ggttggctcc atccaacggg agtaatcacc
     4981 taactctata tcttcgattt ttaaaacctc agcacccagg tctgtaagaa ttaaggtggc
     5041 gtatccacca gggataagtc tagttagatc taaaaccttt aaaccttgaa gcggaagcat
     5101 tattcaaata cgggttttgg aaaccgaatt acgaaataat gtagagacga cccgccgggt
     5161 cgtctctaca attctctacc atcttataac accggaagcc caggtgaaac ctgccccaaa
     5221 gactgagaaa agaattaagt cccctctatc tgccctaccg cttcttactg cttcgtctag
     5281 agcaataggc accgaagcgg aggacgtatt tccatatcta tcaacgttga tgaaaacctt
     5341 ttcagggggg atatcaaggc gctttcttac agcttccatt attctcaaat tcgcctggtg
     5401 aggaatgaat aaatcaatat cgtcaggtgc aatatctgcc tttctgatag cctcaatcga
     5461 ggctgactcc atggctttta cagcttcttt gaatatatcg ttccctttca tttttatata
     5521 atgaagccta ttacgtatgc tttcttcact aggtggtatg cgagagccac cggcaggcat
     5581 gtgaagtagc tcccagtcat caccatttgc gccaaggcat agagacagaa ttcctgacat
     5641 gtcggatttt ccaattagag ctgcgcctgc cccgtctcca aaaagaacgc atgtggctct
     5701 atcgttaaaa tccataatcc tcgtcatagt ctcagcgcct actataagaa taatctttgc
     5761 ctccccggat tctatcattg cctttccaag ctgaagtcca tagacaaacc cggagcagcc
     5821 ggcggagagg tcaaatgcaa atgccctttt ggctcttatg cggctttgaa gaaggcaggc
     5881 tgtggaaggg aaaaaataat caggggtgac agtgccgagt attatgccat ctacatcaat
     5941 ggggtctaca gatgcagctt caagtgctcg aagcgatgct tcataagcca tgtcagatgt
     6001 ggcaacatcg ggatcagcaa tccttctctc gacaattccg gtgcgtgtgc gtatccattc
     6061 atcagaggta tcaacgattt tttcaaagtc gaaattcgtt agtattcttt ccggtgctga
     6121 tgaaccagtg ccaagaatcc ttacaggaat caatgtagtg attactccct agagggtttt
     6181 ggattgatat tatcagggaa agcacagatt ttgtcaatca agctcttgct tgatctagta
     6241 cttctttgat tttctccttc tctcttttag ttatttcaac cctctttgaa tatgagatta
     6301 ttgagtcaaa taaaagtatg tcgaaataat cctgttctgc taactttcct agtaacttca
     6361 tgtcttcatc agattcaatg ttaaagaaat aatcaaacaa aaaaggtttc tttgacttat
     6421 ctcttaaatc gaaatacatt ctcacagatg ggaattcagg tctttttatg cattcaagcc
     6481 caaaaaatat ctggcttgta ttatttatcg actcaatttc gttatgtgat cctttaaaca
     6541 caaaaacagt catgtcattc aacgaagtag caaggcatcc tgattccata aatgatagcg
     6601 tcatcttgat ttcatgcgtc agttcgacac gggttgtttt gtgtatcata agtttgttaa
     6661 gcaatcttat gatgctggat agcaagtaat tctcctatca ggtctaagcg gtcttaatac
     6721 tttgtcattc cgagcagagc gaggaatccg acacttagtg tcgtcctgag cgaaagcgaa
     6781 ggatccgaag ataaatcatg agatccttca gtcgctttga tcctacagga tgacgctatg
     6841 cgtcagattg ctttgtctcc ttcgaccttg gtcggtactg gctccgtggg taatgtaggg
     6901 aacgcatata tgcgttccct acaagtttgt gacgttatta ttataaaatc ataccctttt
     6961 gcttgctgca ttgtatttca tctagtcaga cgattacctc ttttttcatc ctcattagaa
     7021 gccagagaaa aaatacgcca ccgcaggaag ctgtaataat tccaacaggg atttcgatcg
     7081 gggcgaaaat tgttcttgat acagtatcgg cggctgtcag aaatgaggcc ccaagaagaa
     7141 aggaggatgg aagaatgatt ctgttatcaa cgcctataat cattctaagc acgtgcggaa
     7201 ttatgagtcc aacaaaccca atgggacctg ttactgatat aactgcactt acagcgagtg
     7261 atgtggcaaa atagattttc ttttccatca agcctatatt tactccaagc gaggaggcaa
     7321 cgtcgtctcc taatgcgaac aagttcatgt ccttcgtaat agaaagcaga atcacgcagc
     7381 caggaatagc aaatattaag gttttccaaa ctgtgtcaag tcccacaata tctaggcttc
     7441 ccataatcca tcttattgtt tgaagtgatt gaatgaaatc ggagaaaaac tggatgaaga
     7501 gtatcaaagc cgaataaagg aaattaacca caacacctgc aaggagcaat cttccggtag
     7561 caactactcc atgcactttt gaaaccgaat atacaaagag gatggtaaag atagaaaaga
     7621 taaaagaaac aatcgaaata gaagaaaaac caagaaacgt atttcccagt cccaatctta
     7681 tggcgatgat tgcgccgagt gatgcaccac cggaaatacc aagtgtaaaa ggtgcggcaa
     7741 gtgggtttct gaatagagcc tgaagcgaag cacctgcaat tgcgagtgtt cctcc
//
LOCUS       DATDRL010000187         5742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3731831, whole genome shotgun sequence.
ACCESSION   DATDRL010000187 DATDRL010000000
VERSION     DATDRL010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3731831"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VI935_06445"
     CDS             complement(<1..636)
                     /locus_tag="VI935_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HLE25275.1"
                     /translation="MSRLKISVIGAGNVGASAALKLAELELGDVVLVDIIEGMPQGKA
                     LDLMQMRPVSGRDVNIIGTNGYDETEGSDIVIVTSGIPRKPGMSRDDLLSTNTKIVKQ
                     VTAEVAKRSPNSILILVTNPLDAMVYVAYKVSEFPKQRIMGMAGVLDSARFRTFIAME
                     LGVSVENIHAFVLGGHGDDMVPLARCTTVAGVPITELMSKDRVDALIQRTRD"
     gene            complement(736..1995)
                     /gene="icd"
                     /locus_tag="VI935_06450"
     CDS             complement(736..1995)
                     /gene="icd"
                     /locus_tag="VI935_06450"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HLE25276.1"
                     /translation="MSSFTPPNEGQKIKIDQKKNELQIPDNPVIPFIEGDGTGPDIWR
                     ASRRVFDAAVKKAYNGNKKIVWFEVLAGEKAYNKLGTWLPDETLENIKGYIVAIKGPL
                     TTPVGGGIRSLNVTLRQLLDLYACVRPVRYIKGTPSPVKRPEHMDVVIFRENTEDVYA
                     GIEWKSGSPEVKKLLKYLKRNFKVNIREDSGIGIKPISAFGTKRLVRKAIKYALARGR
                     KSVTLVHKGNIMKFTEGAFREWGYELAREEFGHVTVTEDELWTKYDGRMPEGKILIKD
                     RIADNMFQQVLTRPDEYDVIATPNLNGDYLSDACAAQVGGLGMAPGGNIGDFYAVFEA
                     THGTAPKYAGQDKVNPGSVILSGVMMLEYLGWQEAADMIIKAMEKTVSEKKVTYDLHR
                     QLEGARLLRCSEFGDAIISNMDDLPSE"
     gene            complement(2107..4818)
                     /gene="acnA"
                     /locus_tag="VI935_06455"
     CDS             complement(2107..4818)
                     /gene="acnA"
                     /locus_tag="VI935_06455"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HLE25277.1"
                     /translation="MTKNNPFKTITSLKTSLGTVKFYSLERLGNALKVDISRLPFSIK
                     VLIENVLRQCDGNLVTEDDVSAIAHWNAKKSGGREIPFLPARVVLQDFTGVPCVADLA
                     AMRSAVNKIGGDTMRINPIVPVDLVIDHSVQVDYFGSPSAFEANVEREFERNRERYAF
                     LRWAQKAFDKFRVVPPGTGIVHQVNLEYLAKVVQSSGMNGNTVVFPDTLVGTDSHTTM
                     INGLGVLGWGVGGIEAEACMLGQPLYMLIPEVVGFKLTGELVEGATATDLVLTVTQML
                     RQKGVVEKFVEFFGPGLSRLSLADRATVANMSPEYGATTGFFPVDEETLNYLRGTGRD
                     KELVELVEHYTKAQGLFRTDDMPDPIYSDVLELDMRSVEPSLAGPRRPQDRVPLKEMK
                     RSFVNVLKSYLTKQEEGAKSPGSEEKIEVKMNGHKATLTHGSVVISAITSCTNTSNPS
                     VMIGAGLLAKKAVEQGLMVNPTVKTSLAPGSRVVTDYLKKSGLMPYLEALGFHLVGYG
                     CTTCIGNSGPLPEPVADAVKRNDLTVAAVLSGNRNFEGRINPLAKMSYLASPPLVVAY
                     ALAGTVNIDMLNEPLGVNKSGKPVYLRDIWPSQNEIRYAIKKALKPELFRSQYSHVFE
                     GEEQWKKLSTPEGSLYEWDPDSTYIQEPPFFKDLSPEPQPLQDIRGARVLALLGDSVT
                     TDHISPAGDIPADGPAGRYLIDRGVTKREFNSFGARRGNHEVMIRGTFANIRLKNLMV
                     PGTEGGWTIYIPTNEKMSIYEASMKYQAERIPLIVIAGKEYGSGSSRDWAAKGTMLLG
                     VKAVIAESFERIHRSNLVGMGVLPLQFKLGENRETLKLTGFEEYEVDGITQELKPRKE
                     VTVRARRTDGSEIKFKAICRLDSLIEIEYYRNGGILQTVLRQMVKHS"
     gene            5068..5271
                     /locus_tag="VI935_06460"
     CDS             5068..5271
                     /locus_tag="VI935_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015224895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLE25278.1"
                     /translation="MLRYFTLEYWVDEGWYVGRLKEVPGVFSQGESLEELEENIREAY
                     RLMLEEGISPNPEVQTKDIGVGV"
     gene            5268..5420
                     /locus_tag="VI935_06465"
     CDS             5268..5420
                     /locus_tag="VI935_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012267861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HLE25279.1"
                     /translation="MKRRDLIRKLVVAGCYLKRHGKKHDIYANPKNGKKAPVPRTQKL
                     RRPFAN"
     gene            complement(5519..>5742)
                     /locus_tag="VI935_06470"
     CDS             complement(5519..>5742)
                     /locus_tag="VI935_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25280.1"
                     /translation="TIVYCHCERSEAISYFRLLRRFSPRNDSYNGIFLRPYPAACCRV
                     IDYHFIHKRLSQVKIEAFDKKNFQSDNVP"
BASE COUNT         1591 a         1377 c         1084 g         1690 t
ORIGIN      
        1 atcgcgggtt ctctgaatta aagcatcaac cctatcctta gacataagct ccgtaatcgg
       61 aacacctgca accgtagtac accttgcaag tggaaccata tcgtctccgt gtcctccaag
      121 aacaaacgca tgtatatttt caacggacac accaagctcc atcgcgataa aggtcctaaa
      181 cctagccgaa tccaaaactc cagccattcc cattattctt tgctttggga attcgctcac
      241 cttatatgct acgtatacca ttgcatcaag tgggtttgta acgagaatta agattgagtt
      301 tggagatctt ttcgctactt ctgcagtaac ctgttttaca atcttagtat tggttgaaag
      361 aagatcatca cggctcattc ctggtttacg aggaatgcct gatgttacta ttacaatatc
      421 tgacccttct gtctcgtcat aaccatttgt cccgataata ttaacgtcgc gcccggatac
      481 aggacgcatc tgcattaaat caagcgcctt tccttgaggc ataccttcaa taatatccac
      541 tagaacaaca tctcccagtt cgagttccgc taattttaag gcagcagaag cacctacgtt
      601 tcctgctcct attaccgaaa ttttaagtct gctcatcgtt tttcctccct gatttactta
      661 ctttttcaat tgtttttctc taaatgaacc ttaaataata ggtataatta aaaccgcttt
      721 ttccaaataa gctttctatt ctgacggtaa atcatccatg ttagatatta ttgcatctcc
      781 aaactcggaa cacctgagaa gtctagcacc ttcaagctga cgatgtaggt cgtaagtgac
      841 ctttttttca gaaactgttt tctccatggc tttaatgatc atatctgcag cctcttgcca
      901 gccaagatac tcaagcatca taacacccga aagtattaca gagcctggat tcaccttgtc
      961 ctgtcccgca tattttggcg ctgttccgtg tgttgcttca aacacggcat aaaaatctcc
     1021 aatgtttcca ccaggagcca ttccaagccc acctacctgg gcagcacatg catcggagag
     1081 ataatcacca ttcaggttag gtgttgcaat tacgtcatac tcatcaggtc ttgttaaaac
     1141 ctgctggaac atattatccg caatcctgtc tttgattagg atcttccctt caggcattcg
     1201 gccatcatac ttggtccaga gttcatcctc tgtaaccgta acatgaccaa attcctcgcg
     1261 tgcaagctca tagccccact ctctaaacgc accttcagta aacttcatga tgtttccctt
     1321 gtgaacgagt gttacgctct ttctacctct tgcaagggcg tacttaatag cttttctaac
     1381 aagcctcttt gtgccaaaag cgcttatcgg tttaattcca attccagaat cctcgcgaat
     1441 attcactttg aaattccgtt ttaaatactt taaaagcttt tttacctcag gagagccgct
     1501 tttccactcg atcccagcat aaacatcctc agtgttttct ctaaaaatca ctacatccat
     1561 atgttcaggt ctttttacgg gagaaggagt cccttttatg tacctaacag gacgcacaca
     1621 agcataaaga tcaagaagtt gccttagcgt aacgtttaaa cttctgatac cgccaccaac
     1681 cggagttgta agaggtccct ttatggcgac tatatatccc tttatattct caagcgtctc
     1741 atccgggagc caggttccaa gtttattgta tgccttttca ccagcaagaa cctcaaacca
     1801 aactatcttc ttgtttccgt tatatgcctt tttaactgcg gcatcaaaca cacgccgaga
     1861 agcccgccat atatcgggtc ctgttccgtc accctctata aatggaatga caggattgtc
     1921 tggaatttga agttcattct ttttttgatc aattttgatt ttttgacctt cgtttggtgg
     1981 tgtaaacgac gacatgacgt aattcctctc ttatatattt ctaggaagtt gaatatacat
     2041 ggatttgtta aaaaaacaaa tttaagtcga agggaaattg aaactcagtg tttatcttat
     2101 cgtgattcag ctatgcttaa ccatttgacg aagcacagtc tggaggattc caccgttacg
     2161 ataatactct atttcaataa ggctatcgag acggcatatt gctttaaatt taatttcact
     2221 accatcggtt cttcttgccc taacagtaac ctccttacga ggtttaagct cttgagtaat
     2281 tccatctacc tcatactcct caaatcctgt tagtttgagt gtctctcggt tctcacctag
     2341 cttaaactga agtggaagta cacccatccc caccaagttg cttcggtgga tcctttcaaa
     2401 actctcagcg attactgctt taactccaag taacatggta ccctttgccg cccagtcgcg
     2461 tgagctacca cttccgtatt ccttcccagc tataacaatg agtggtattc gttcagcttg
     2521 gtatttcatc gaagcctcat agatactcat tttttcatta gttggaatat atatagtcca
     2581 gccaccttca gtgcccggca ccattaagtt cttaagcctg atattagcaa aggtgccacg
     2641 aatcataacc tcatggttac cgcgtcgcgc gccaaagcta ttaaactccc tcttagttac
     2701 accacgatca atcagatacc ttccagccgg tccgtctgcc ggaatatcgc cggcgggtga
     2761 aatatgatcg gtagtcacag aatcaccaag taatgcgagg acgcgtgccc ctctaatatc
     2821 ctgtaacggt tgtggctccg gcgagagatc tttaaagaat ggcggctctt gaatataggt
     2881 tgaatctgga tcccattcat acaagctacc ttctggagta gataatttct tccattgctc
     2941 ttcaccttcg aagacatgag aatactgact tctaaaaagc tccggtttca gagctttttt
     3001 aatcgcgtaa cggatctcat tctgacttgg ccagatatca cgcaggtata ctggtttacc
     3061 acttttatta acgcccaaag gttcatttaa catgtcaata ttcactgtcc cggctaacgc
     3121 atatgcaact acaagcggcg gcgatgcaag atagctcatt ttggcaagcg ggttaatacg
     3181 cccctcaaag tttctgtttc cactcagaac tgcagcgacg gtgaggtcat ttctcttaac
     3241 agcatcagct acaggttcag gtaatggccc actatttcca atacacgttg tgcaaccata
     3301 accgactaga tgaaacccca gtgcctctag ataaggcata agccctgatt tcttaagata
     3361 gtctgttaca acacgcgatc caggcgccaa gcttgttttt acagtagggt tcaccataag
     3421 cccttgttcg actgcctttt tagccaataa ccctgcgccg atcatgacag atggattaga
     3481 tgtattagta caactcgtta tagccgaaat caccacagag ccatgggtca gtgttgcctt
     3541 gtgtccattc atcttcacct cgatcttttc ctcactgcct gggcttttag caccctcctc
     3601 ctgcttcgta aggtaactct ttagtacatt tacaaaagac cttttcattt ccttaagagg
     3661 tactctgtcc tgcggccgtc taggaccagc gaggcttggc tcaacagatc tcatgtccag
     3721 ttctaaaacg tcactgtaga ttggatctgg catatcatca gttctgaata gtccttgtgc
     3781 ctttgtatag tgttcaacta attcaacaag ttctttatca cgaccggttc cacgtaggta
     3841 attaagagtt tcctcatcta ctggaaagaa acccgttgtt gccccatact ccggtgacat
     3901 attagccaca gttgctcggt ctgcaaggct taggcgactt agacctggac caaagaattc
     3961 gacaaacttt tccacaacac ccttttgtct caacatctgc gtcacggtaa gcacaaggtc
     4021 ggtagccgtt gcaccttcta ccaactcgcc ggttagtttg aatccaacaa cctccgggat
     4081 aagcatgtaa agtggctggc ctaacatgca ggcttcggct tcaattcccc ctacacccca
     4141 acccaataca ccaaggccgt taatcatcgt tgtatgagaa tcggttccaa caagtgtgtc
     4201 gggaaagaca actgtattac cgttcattcc gctggactga acaacctttg ctaggtattc
     4261 gagattgacc tgatggacaa tgcccgtgcc cggcggcacc accctgaatt tgtcaaacgc
     4321 cttctgcgcc cacctaagga atgcatatcg ttcacggttg cgttcaaact ctcgttccac
     4381 attagcctca aaagcggagg gcgaaccaaa atagtcaacc tgcaccgaat ggtcaatcac
     4441 tagatcaaca gggacgatag ggtttatccg cattgtgtct ccgcctattt tattcacggc
     4501 agaacgcatt gctgccaaat ctgcgacgca aggaactcct gtaaaatcct gtagtacaac
     4561 acgtgcaggt aaaaatggta tctcccgtcc tccggatttt ttagcattcc aatgggcaat
     4621 tgcgctaaca tcatcctctg taacaagatt accatcacac tgcctaagaa cgttttctat
     4681 aagaacctta attgaaaagg gcaatcgtga aatatcaact tttaaggcat ttccaagtcg
     4741 ttctagggag tagaacttaa ctgttcctaa gctggtttta agggacgtaa tagttttaaa
     4801 aggattgttt ttagtcatta cttttcgcca atcatgattc aagcttacaa atacacctat
     4861 ttgtattata taccaggagt gtaaataaga aaattcattc ttaaaaattt atcgattctt
     4921 aaaatcttag taaaattaag ttttactgaa ccaaaactta gcatatactt taatttaaca
     4981 caccggtaaa cttaacaaaa tggcattcaa taaagattcg gctgttttaa ttttaccgat
     5041 acaaattctg caaaaaggag acaaattatg ctaaggtatt tcacacttga gtactgggtt
     5101 gacgaggggt ggtatgtagg taggctgaaa gaagttcctg gcgtgttcag ccaaggtgag
     5161 tctcttgaag aattagagga gaacatccgc gaagcatacc gactgatgct cgaagaaggg
     5221 atttcgccca atcccgaagt tcaaaccaag gacattggag tgggggtgtg aaacggcgtg
     5281 atcttatccg taagctagta gttgcaggat gctatcttaa gcgtcatggt aagaagcacg
     5341 acatatatgc aaatcctaaa aacggtaaga aagcaccagt tcctcgcact cagaaattaa
     5401 ggagaccctt tgcgaactga taaaaaaaca gcttggactc aaatgaattg tatgctgatt
     5461 gtttttatct cggtgtcgga gtcataatgc tttatgaggt aagggttgat cgagaagctt
     5521 atgggacatt gtcggattga aaattctttt tgtcaaaagc ttcgatttta acttgcgata
     5581 aacgcttatg aataaaatga taatcaataa ccctgcagca agctgcaggg tatggtctta
     5641 aaaatatacc gttatagctg tcattgcgag gagaaaagcg acgaagcaat ctaaaataag
     5701 agattgcttc acttcgttcg caatgacaat acactattgt gg
//
LOCUS       DATDRL010000188         3847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_801378, whole genome shotgun sequence.
ACCESSION   DATDRL010000188 DATDRL010000000
VERSION     DATDRL010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801378"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VI935_06475"
     CDS             complement(<1..432)
                     /locus_tag="VI935_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HLE25281.1"
                     /translation="MSVSAKLRDYLDQNMIKYTLITHSKGFTAQELAAIMHVPGKEMA
                     KVVILKIDGRFAMAVIPAPHKIDFERFKVATGIKEVALASEKEFQNLFPDCEVGAMPP
                     FGNLYGLPVYVAQPLKEDKEIVFNAGSHTEAIRMQYADFERL"
     gene            complement(585..1028)
                     /locus_tag="VI935_06480"
     CDS             complement(585..1028)
                     /locus_tag="VI935_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLE25282.1"
                     /translation="MSLGNLCRREVVVVNQGTPIKEAVKFMEEKNIGSVIVVGNGKPL
                     GIATDRDILLRVVNKGLDPEKSPVDDVMTKEIVTLKEEMGLFEALEQVKGKGIRRFPI
                     VDARGNLKGIMTLDDIIYLLGKEMADVASIIESPLETTNWRLSIK"
     gene            1388..1951
                     /locus_tag="VI935_06485"
     CDS             1388..1951
                     /locus_tag="VI935_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25283.1"
                     /translation="MRIYIVIVLFITVSFLNYAGAKDEKEPVLISAEELCSKLSSDEN
                     AFKTYKGTEITIYGKLSSVGDKRKAYLGNRMDSVIDLVITPRPKKEPFSEDFTHTDCY
                     ISCLVNVPGEHCEYRDGRYECKEITNTRLEENRRIILKLSGGESITVKGEPENMSLIP
                     LYSIGGHKKQIVNIILSKCEIVQIERK"
     gene            2033..2437
                     /locus_tag="VI935_06490"
     CDS             2033..2437
                     /locus_tag="VI935_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HLE25284.1"
                     /translation="MKFKKIERDFISLQRLIRIATVDKNGTPHNVPVCHVSEGNHIYF
                     ATGKESKKIKNLEENGKVALVCDEYSEIWSYLKGVFVQGKTRVISKGPEFRKLKKMLY
                     QKYTLYEAEAPIEEENTVIVEIIPQNIVSWGL"
     gene            2516..2977
                     /locus_tag="VI935_06495"
     CDS             2516..2977
                     /locus_tag="VI935_06495"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLE25285.1"
                     /translation="MIVREATKRDVRACAKIEKQESGESIEIIEDEIEEQVHDENHFI
                     FVAEDDKRVVGFISSEWQKWNSSLYIDSLYIDKNYRFKGYGSNLLKQMIRKSKEIKSK
                     KVFVDVEAGNRDALILYLKHGFEINGYIQDFYRSKEEGDAVFLVLYNKYSS"
     gene            3395..3721
                     /locus_tag="VI935_06500"
     CDS             3395..3721
                     /locus_tag="VI935_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25286.1"
                     /translation="MLNIFVKNIFLVTEVIGAFKNKEVRSKRGCPMFDIKIYENEKEI
                     ETIKNLTYTQKEALERVLTTVSNRIEGFGFVPEHIETRSKQFGRRKVAYLHFDQKEGS
                     WMRRSK"
BASE COUNT         1282 a          676 c          745 g         1144 t
ORIGIN      
        1 caatcgctcg aagtctgcat attgcatgcg aatagcttca gtgtgactcc ctgcgttgaa
       61 aacaatttcc ttgtcttctt ttagtggttg tgcaacatag acaggcagac catataaatt
      121 accaaagggt ggcatagcac cgacctcaca gtcgggaaag aggttttgaa actctttttc
      181 agaggctagc gcaacctctt tgatcccagt ggcaacttta aacctctcga aatcgatctt
      241 atgcggtgcg ggtataaccg ccattgcaaa tcggccatcg attttaagaa taacaacctt
      301 agccatttcc tttcctggaa catgcatgat agcggccagt tcctgagcgg tgaatccttt
      361 tgagtgggtg attaaagtgt acttaatcat gttctgatcc agataatctc ttaatttcgc
      421 tgaaacagac attttcgtac cctcctttat aggagataaa ccttcagaat aatctctata
      481 aatataaaat agttgttata ttgatagtta cttgcttcga atacactgct acttataacc
      541 tagggccctc cctctcaatt atgctagcca catcagccat ctctttactt tatgctcaat
      601 ctccagtttg tcgtctccaa tggactctca attatactag ccacatcagc catctcttta
      661 cctaacagat aaataatatc gtcaagtgtc ataatacctt ttaagttccc cctagcatct
      721 acaataggga acctccttat accctttccc ttcacctgtt cgagggcttc aaatagaccc
      781 atctcctctt ttaatgttac aatctcttta gtcattacat catcaacagg actcttttcg
      841 ggatcaagcc ccttattcac aactcttaga agaatatccc tatctgttgc tatcccaagt
      901 ggctttccgt tcccaacaac tatgacactc cctatattct tttcttccat aaattttact
      961 gcttctttta tgggggttcc ttggtttaca accacaacct ctctacgaca cagattccca
     1021 agactcataa tatcgtacct cctttcacaa aaaatttttc tttttctata catccaactt
     1081 gcaagccata tgccacttaa tccttcatat ataacttgtt gaaatttata agaattccat
     1141 tgggaaatac tccatactaa aaacacatgt ttgtcagaat ttaagacatc ggacacacgg
     1201 tttattggta tctacacagc cttctatcat ttctatacat cttccatatg ttgtttgata
     1261 cttgttgatc taccaattga atttataaac aaacttttaa attagttgaa agggttcata
     1321 gttggtgaga taatttttag aaaagataag tttacctatg atttgtccat aggactgaaa
     1381 catcaaaatg agaatataca tcgttattgt actatttata acggtttctt ttcttaatta
     1441 tgctggcgca aaagatgaga aggaaccagt tttaatatca gcagaagagt tatgttctaa
     1501 gctctcgtct gatgagaacg ctttcaagac atacaaaggc acagaaatta caatttacgg
     1561 caaattatct tcagttggtg ataaaagaaa agcatattta gggaatcgca tggattctgt
     1621 aattgatttg gtaataacgc cacgaccaaa aaaagaaccg tttagtgaag attttacaca
     1681 tacagattgt tatatctcct gtctcgtcaa tgtacctgga gaacattgtg aatatcgcga
     1741 tggaaggtat gagtgtaaag aaataactaa cacaaggctt gaagagaata ggaggattat
     1801 ccttaaactt agtggaggtg aatctataac cgtgaaggga gaacctgaaa atatgtcgct
     1861 gattccatta tattctatag gaggtcataa aaaacaaatt gttaacatta tcttatcgaa
     1921 gtgcgaaata gtacaaattg agaggaagta agcgagccac tcgaggagca tattattaaa
     1981 cgagatctta aaacgtgggt tccaaaaatc cattagggag gtcatgactt agatgaaatt
     2041 taaaaagata gaaagggatt ttatttccct acagcgttta atcaggatag ctacagttga
     2101 taaaaacggc acgcctcata atgttccagt gtgtcatgtc tcggaaggaa atcacatcta
     2161 ctttgctact ggaaaggagt caaaaaagat taagaatctt gaggaaaatg gtaaggtagc
     2221 tttagtatgc gatgaatata gcgaaatctg gtcttatctc aagggggtat tcgtccaggg
     2281 caagactagg gttatatcca aagggccaga gtttcgtaag cttaagaaaa tgctttatca
     2341 gaaatacaca ctatatgaag cagaagctcc gattgaagag gagaacacag tgatagttga
     2401 gattattccg caaaacattg taagctgggg gctttgagca aaagaacggt tcgggtttaa
     2461 ttctcaaaat cgattgacca tcccattatg ataaagttta agagggtcta gtattatgat
     2521 agtaagagaa gctactaaaa gagatgttag agcatgtgca aaaatagaaa agcaggaatc
     2581 aggtgagagt atagagataa tagaggatga aattgaggaa caggtccatg atgagaatca
     2641 ttttattttt gtagccgagg atgacaaaag ggtggttggc ttcatatctt cagaatggca
     2701 aaagtggaat agctcccttt atatagatag cctctatata gataaaaact ataggttcaa
     2761 agggtacggc tctaaccttt taaaacaaat gataaggaaa tcaaaagaaa tcaagtccaa
     2821 gaaggtcttc gtcgatgttg aagcaggcaa tagagatgca cttattcttt atttaaaaca
     2881 cgggtttgaa ataaatggtt acatccagga tttctacagg agcaaagaag agggagatgc
     2941 tgtttttcta gtactttata ataaatattc aagttagaat agaagcagaa gattgaacac
     3001 ggcattcaca gaaatcagca tctcaaatgt gctatgtaag ttaagatcaa ttttagttca
     3061 acaaaaaaaa tcacagagtc aaaaaatttt cttgactttc atatccatcc ggagtattgt
     3121 agaaatataa ttaaattaaa ataggtgggt gacgttaaga tggggtagat tatatacggt
     3181 atagagattg ttgtcttctt tactcagcaa aggttttttc actaaatacg gatttaatta
     3241 accaagtttt atgcattctg aataattcca gaaatatctt tacctgcctt cctaaatata
     3301 aaaatacata cagcattcac cctccttaag agaggttttg ttcattcctt ttcttaaggt
     3361 ttttaaaatg accatgagag gaggtggatg gtaattgcta aatatttttg ttaagaatat
     3421 tttcctagta acggaggtta ttggagcttt taagaataaa gaagtacgta gtaaaagggg
     3481 gtgtccgatg tttgatataa agatttatga aaatgaaaag gaaatagaaa ctataaagaa
     3541 tcttacctac actcaaaagg aggcacttga gagggttctt acaacagtat ccaatagaat
     3601 agaaggattc ggattcgtac cagagcatat tgagacgaga tccaagcagt tcgggcggag
     3661 aaaggttgcc tacctacact ttgatcaaaa agagggttct tggatgagaa gatctaagtg
     3721 agcaattacg tgggaggggt ggctgtttta tgaagaactt gaagaaaaaa aagaccggtc
     3781 ggcacaaaca atcatcgtca agaaagggag tggacgtaac tcagtgcgac tcggaacaat
     3841 tgagcga
//
LOCUS       DATDRL010000189         4094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2835204, whole genome shotgun sequence.
ACCESSION   DATDRL010000189 DATDRL010000000
VERSION     DATDRL010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2835204"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VI935_06505"
     CDS             <1..445
                     /locus_tag="VI935_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HLE25287.1"
                     /translation="HQFNKVELVKFVTPENSYPELESLTNDAARVLELLELPHRIVVL
                     STGDMGFSSAKTYDVEVWIPTENTYREISSCSDFEDFQARRANIRYRPKGGGKPRFLH
                     TLNGSGLAVGRTVVALLENRQEYDGSVVIPTALIPYMGGVDIISS"
     gene            530..1303
                     /locus_tag="VI935_06510"
     CDS             530..1303
                     /locus_tag="VI935_06510"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HLE25288.1"
                     /translation="MKEYAVELAKRAGTALIQGFKSLAFIKNESKDPRHLVTKFDLES
                     EKIIIGGIKRKYPHHNILSEEAGIINKGSYYTWIVDPLDGTGNFTKKNPFFSISIALR
                     YKKELILGIVYVPVLDELYSGEKGKGAFLNNTPITVSSTKELNRSSIVSCEGSDKNII
                     RCAKLFREIRPLVLDMRKLGSAAIESAWVACGRAEAYIVTKINPWDIAAGVILVEEAG
                     GKVTDFKGRSWSVNKSDPILSNGKIHEKLLVKINKGLKS"
     gene            1403..2530
                     /locus_tag="VI935_06515"
     CDS             1403..2530
                     /locus_tag="VI935_06515"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HLE25289.1"
                     /translation="MNNSSHTDYDVIIVGTGPAGGMCAYELSKRNLKVLLLEKEKLPR
                     YKVCAGGLTKKAVDILPEDIRDIAENYTFNTHLTLNHRWRFLKATSFPIVTMVMRDKF
                     DNFLVQKSQAYGASLLDRTRVNSVEEFPDYVLVRTERGNFKSKVIVGADGVTSLVARS
                     LGLRKKPKLGVALEGEIFPNNNSVDLSSYNGSLHLDFNVLPKGYGWIFPKRDHLSIGV
                     FTTLPRIKEVKRYFSFYLERKGLAKNYTCRSLVGHQIPIGGCDEVLNTTRGILIGDSA
                     GLADPITGEGIYFGLRSGQIAAEVIAQSLTTDSILLDEYTQKVKSEIVADFKIASYMA
                     KFFYNLSFFTYNIVRKTNKFSEAFVRVVAGDQSYRDMFKNI"
     gene            complement(2843..3883)
                     /gene="rtcA"
                     /locus_tag="VI935_06520"
     CDS             complement(2843..3883)
                     /gene="rtcA"
                     /locus_tag="VI935_06520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HLE25290.1"
                     /translation="MKFVVIDGSFGEGGGQILRTSLSLSIITGKPFEIINIRARRENP
                     GLRPQHLASAHSAARISSAFLEGAELGSLRLRFIPKEVEPGEYTLDVSTAGATSLVLQ
                     TIFFPLALKGKSGSIANIRGGTHVHWSPSFEFLEEEWIPFMRRIGFQGEVRLKRAGFY
                     PKGGGEIISSIEPIKVVLPLVIKERGNLIKVIGISAVGDLTKRVAERGKERLIQILNE
                     YNIYNQVESRDLPSFGRGAIVFLKAVFENTIVCYSALGERGKRMEEVSEEASQKLFNF
                     LKSDATVDENMGDQLLLPLALAHGNSFFKVPRITEHITTNTEIIKKFLNEGRIHFEGN
                     EILVEGIGGIDR"
     gene            complement(3902..>4094)
                     /locus_tag="VI935_06525"
     CDS             complement(3902..>4094)
                     /locus_tag="VI935_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25291.1"
                     /translation="MLSALTSMFCFLFLVVFLFRYAFESVSEQEQTIAYYFFIILLVG
                     LLMVPISLFILERIERIKD"
BASE COUNT         1335 a          796 c          770 g         1193 t
ORIGIN      
        1 acaccaattt aacaaggttg agcttgtaaa atttgtaacc cctgaaaatt cctaccctga
       61 gcttgaatct ctaacaaacg atgccgctcg cgtgcttgaa ctccttgagc ttccacatag
      121 aatcgtagtt ctctcgacag gggatatggg attttcatct gctaaaacct acgacgtaga
      181 ggtttggatt ccaaccgaaa atacatatag agagatctct tcttgcagcg actttgaaga
      241 ctttcaggca agaagagcta atataagata ccgtccaaaa ggcggaggaa aacctagatt
      301 tcttcacaca ttaaacggct ccgggctagc tgttggtaga acagttgttg ctctcttaga
      361 aaatcgccag gaatacgatg gttcggttgt aattccgaca gctcttatcc cctatatggg
      421 tggtgtagat ataatctctt cttgaataaa taaatcagtt gcaggacaag aaaatcccac
      481 ctaatattgt ttgcgcacta ttttacttgc aagtacaaag gggcgctata tgaaagaata
      541 cgccgttgaa ttagctaaaa gagcaggaac tgctctgata cagggtttta aatctttagc
      601 cttcattaaa aacgaatcaa aggaccctag acacttagta accaaatttg atttagaatc
      661 agaaaaaatc attatcggcg ggataaagag gaaataccca catcacaaca tactttcaga
      721 agaagcgggg atcataaaca aaggttctta ttatacatgg attgtagatc ccctagatgg
      781 cacaggaaat tttacaaaga aaaatccatt ttttagcatt tcgattgcgc ttcggtataa
      841 aaaggagctc attctaggaa ttgtttatgt ccctgtctta gatgaacttt actcgggaga
      901 gaaaggtaaa ggggcatttt taaataatac cccgattact gtttcttcaa ccaaggaatt
      961 aaacagatca tcgattgttt cttgtgaagg ctcagacaaa aatattatca ggtgcgcgaa
     1021 actctttcga gaaataaggc ccctagttct tgatatgaga aaactaggct cggctgcgat
     1081 tgaatcagca tgggttgctt gtgggagggc agaggcctat atagttacaa aaatcaatcc
     1141 ctgggatata gcagccggag ttatacttgt agaagaggca ggaggaaaag taacagactt
     1201 taaagggcgc agttggagcg taaataaatc agacccaatt cttagcaacg ggaagataca
     1261 cgagaagtta ttggtaaaga taaacaaggg cttaaaaagt tgatagtttt tgatttatta
     1321 gttggggcag accccccgtg tctgccctaa tgaaacacac aataaggagc cgggcgaaca
     1381 cttaggttaa ctcctacaat agatgaataa ttcttcgcat accgattatg acgtaattat
     1441 cgtaggaaca gggccggcag gtggtatgtg cgcctatgaa ctgtcgaaga gaaatctaaa
     1501 agtgctttta ttagaaaaag aaaaactccc aaggtataag gtttgtgcag gtggattaac
     1561 aaaaaaagct gtagacattc tacctgagga tatacgcgat atagcagaaa actatacctt
     1621 taatacccat ttgactctca atcacagatg gcggttttta aaggcgactt cttttccaat
     1681 agtcaccatg gtaatgagag acaagttcga taatttcctc gtccaaaaat ctcaagcata
     1741 cggcgcatct cttcttgatc gaacaagagt aaattcagta gaagaatttc cagattacgt
     1801 gcttgtgagg acagaaagag gtaattttaa gtcgaaggta attgtaggag cagatggcgt
     1861 gacaagcctt gtggcacggt cattaggact taggaaaaaa cctaaacttg gcgttgccct
     1921 agaaggcgag atttttccaa ataataattc tgtggactta tcaagttata atggctccct
     1981 tcacctggat ttcaatgtct tacccaaggg ctacggttgg atatttccca aaagagacca
     2041 tctctctatt ggagttttta caaccctgcc aaggataaaa gaggttaaaa ggtatttctc
     2101 tttctaccta gaaagaaagg ggcttgctaa aaactacacc tgccgctctc ttgtaggaca
     2161 tcagatccct attggaggat gtgatgaagt tctaaataca acaaggggga tacttatagg
     2221 agactcagca ggactcgccg atccgataac aggtgaaggt atatacttcg gacttaggag
     2281 tgggcagatt gcagcagagg tgatcgctca aagtttaact acagactcta ttttgctaga
     2341 cgaatataca caaaaagtta aaagtgaaat cgtagctgac ttcaaaattg cgagttatat
     2401 ggctaagttt ttttataacc ttagtttctt tacatataac atagtaagaa aaacaaacaa
     2461 attcagcgaa gcattcgtta gggtagtagc aggagatcaa tcctacaggg atatgttcaa
     2521 aaatatataa ggtcttaata aggtcaaatt gacttattaa aaataactgt atgtttttta
     2581 aaggattatt ttaaaatttg ttgatagtta ttcaaagttc gtatatatct ttaaaaagtg
     2641 tggataaagc ctgatattta tttaatgtca attctagtta atttatataa ctaactgtaa
     2701 ctaagtaata gttcccctca gcaattccaa tttaagaata catttatctg ccatattttt
     2761 ctagttttga atcaagtgcc ataaggtccc tatcttatat aattaccatc tataatcctg
     2821 acatattaac taaagggaag agttatctat cgataccccc gattccttca actaaaatct
     2881 catttccttc aaaatgaatc ctaccttcat tcaaaaattt tttaataatt tcggtattag
     2941 ttgttatatg ctcagtaatt cttggaacct taaaaaatga attaccatga gctaaagcaa
     3001 gcggtaggag caactggtca cccatgtttt catcaactgt agcgtcgctt tttagaaaat
     3061 taaacaattt ctgactcgct tcctctgata cttcttccat tcttttccct ctttctccaa
     3121 gcgcgctata gcacacgatt gtattttcaa acacggcttt taaaaataca atcgcacctc
     3181 gtccaaatga tggtaaatct cttgattcta cttgattata aatgttgtat tcgtttagaa
     3241 tttgtattaa cctctcttta cctctttccg caacccgttt tgtaagatcc ccaactgctg
     3301 agattccaat tacttttatt aaattacctc tttctttaat caccagcggc aaaacaacct
     3361 ttatcggctc aatgctagaa ataatttctc ccccaccttt tggataaaaa cctgcacgtt
     3421 tcaatctcac ctccccctga aaacctattc ttctcataaa cggaatccat tcctcttcaa
     3481 gaaattcaaa agacggactc caatgaacat gagtgcctcc ccttatatta gcaatggaac
     3541 cagattttcc cttgagagcc agtggaaaaa atattgtctg gagaacaagg gatgttgctc
     3601 ctgcagttga tacatcgagt gtatactctc caggttctac ttccttcgga ataaatctta
     3661 gcctaagtga accgagttcg gctccctcta aaaaagcaga gcttatcctt gccgctgagt
     3721 gtgctgaggc aaggtgttga gggcgtagcc cagggttttc ccttcgtgcc ctaatattta
     3781 ttatctcaaa tggctttcct gtaatgatcg aaagcgagag ggaagttcta agaatctgac
     3841 ccccgccctc tccaaaggat ccgtctatca ctacaaattt cataataaca gctagttttc
     3901 cttaatcctt aatcctttct attctttcca aaataaagag agatattgga accatcaaaa
     3961 gacccactaa caaaataata aaaaaatagt atgcaattgt ttgttcttgt tcagatactg
     4021 attcaaaggc ataacgaaaa agaaaaacaa caagaaataa aaaacaaaac atggatgtta
     4081 aagctgataa cata
//
LOCUS       DATDRL010000190        13136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1113693, whole genome shotgun sequence.
ACCESSION   DATDRL010000190 DATDRL010000000
VERSION     DATDRL010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13136
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113693"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1968)
                     /locus_tag="VI935_06530"
     CDS             complement(<1..1968)
                     /locus_tag="VI935_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLE25292.1"
                     /translation="MKILLVDDNKENLYMLGTLLRDNGYEVEFAADGLEALEKALQDE
                     FDMIISDIMMPRMDGFQLCRKIKTNERLRRLAFVFYTAAYTDPKDEEFALSLGAEKFI
                     IKPQKPDAFIEILREIIRSHESGMLVAPKLPVEEEAVYLKEYNGRLIKKLEDEVLQLE
                     RKNRYETIIRTVTQGVHRSIELQEVLENAVESMSKNIDRVENISIYLVEGEEAILKAY
                     RGYSDWWVKRVGRIPYPKGFTWKAIIEGKPRYVADAEKDTFIGPAGRELGTKSYLSMP
                     IHLEGKTVGCINVNSFQKNAFDEEELNLLEIVAQQIEVAINNAKQAEALRQSEEALRE
                     NLVQLSKKNRYETIISTVTRSVHQSINLQDVLENAVESMSKNIDGVDNVCIYLVEGKE
                     AVMKTYRGYPDWFIERVRRIRYPKGFTWKTIIKGKPMYCADVDQDTVIGAAGRKVGTK
                     SYASMPIRFDSRTIGCININSFKKDAFDEEELKLLEIVAQQIEVAINNAQQDEALRQS
                     EERYRTLFDQSPVGVYMFDKDFKITQCNERMVEILQSSYDKLIGLDIRKLKDQSFMPA
                     MKKVLKGQSCRQESFYEVTSSSAKLWLSVSLSPLRDADGNVIGGMGVVEDITERKESE
                     QKIQLYTERLQTLSHQLMEIQEIERRHIAHEL"
     gene            complement(2000..2182)
                     /locus_tag="VI935_06535"
     CDS             complement(2000..2182)
                     /locus_tag="VI935_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25293.1"
                     /translation="MWILAATNRTEMLDAALLRPERFDLILELPIPDERLRLEILKIH
                     TEVCLVLSVQQSEAGS"
     gene            complement(2205..2486)
                     /locus_tag="VI935_06540"
     CDS             complement(2205..2486)
                     /locus_tag="VI935_06540"
                     /inference="COORDINATES: protein motif:HMM:NF014929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25294.1"
                     /translation="MGKLLDGLPLVAGDRIRAAFFGSRYQDFIALNTIPHYVYVVVIH
                     PQTIIEIEERKEEGAKKLRITYEDIGGLSHEIQKIKGNGRTSPEASSGL"
     gene            complement(2726..3169)
                     /locus_tag="VI935_06545"
     CDS             complement(2726..3169)
                     /locus_tag="VI935_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLE25295.1"
                     /translation="MSNQWRFTEGLINKWLWNQIPKEKKVLVVDNDEVICDLLKNIIS
                     SDGHRVVAVQNGVEAIKWIKISTFDLVFLDLAMPDISGVEVLKQIKEINSNLPVVIIT
                     GYPDSELMDQVLELGSISVVKKPFTKNQISSVIKSVFALYYRKSR"
     gene            3370..5145
                     /locus_tag="VI935_06550"
     CDS             3370..5145
                     /locus_tag="VI935_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25296.1"
                     /translation="MLLLPLLSVSSKAVSQQEQIEALKKQIEEIGRQNQKQIEELQRQ
                     NQEQIEQLQRKIEELEAERKTEQKKIEEVVTKKEAEEKEAWWKKLRLIGFNPDFPSYF
                     RIRSMFFKKATFVGAVPGKDDDIFFVDSRLLISPMLKITDNLSIRAQIDVFKNIIWGG
                     LGDASVADKFFEAPSPADSFRGALLREVIDTISGNVLSPVEEVDFFDIRSLYLVARVP
                     IGELWVGRQPYDWGLGVLANAGSMPDQDLGSIIDRFEFDTAPLALLDERWENLTFAFL
                     IDRLSQGMSLVNGASGSGWEVGFGALYRVENLQLGGYIYLLSQNNFDISGGLTGDLDN
                     NVNWSIYANYNYDPFFFSLEFQDLFGKVNDLEEPLPSILGNNDIDISPENFLIAARVG
                     YDPPPAFIDFIVAEFGWANGNDAKTPDKLEGNAIFFNNAYTVDNLLFKHIIPNIYALE
                     GSVINSGYVRAWSTLKLNDSFYFTPQALFAWVDERNALSLDLLTPLPKVGRFLGAEIE
                     GTLTWRIIDHLWFDLIGSVVIAGDGLKDLFSQRAFIEGVVPSIGTARPPDAPFAFQGR
                     FVVTIDSLIKTWTGSSSLLKRAYYY"
     gene            5504..7141
                     /locus_tag="VI935_06555"
     CDS             5504..7141
                     /locus_tag="VI935_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLE25297.1"
                     /translation="MPITLERRKCLRTPLVGLEIFSRNTGELIGMSRDITPYGMFIET
                     KNLLVPGIKILLEFKLPGQVIPIKAYSEVKWVRRSTTLEAGPSGMGAHFLGIYESDKQ
                     RLAKYSESLAKQEKINSDNFTLVDFLKVSGDDLFMKTKPVWGYIEDMKQKGFYTYRRP
                     LLSACRNRVVIFDETTGKEREMIMMGSNNYLGLTAHPKVIRAAEEAIEKYGTGAGSVP
                     LLAGTYYLHKELEAKLAKLKGCEDAVIFPTGYSTNVGCLSALVKEKDLAVLDRLTHAS
                     IIDGCMMSSGKFRTFQHSDMQNLREVLERNKDSFDGKLIAVDGVYSMDGDAAPLPEIV
                     DIAQEYGARVMVDDAHATGVIGERGRGTASHFKMVGKIDIVMGTLSKALGQLGGFVAS
                     SKEVVNYLRCFARSYFFSSSLPPVVVASVLAAIEVMESEPELHQNLWKNIKYFKENLQ
                     YLGFNIRNSESAIIPIIIGDELLLRKMSKRIHEEGIYINPIPYPAVPRNQTRFRASIM
                     ATHTREDLDTTLEVLEKVGREFGVIKKGLAIFSRKAA"
     gene            7173..8183
                     /locus_tag="VI935_06560"
     CDS             7173..8183
                     /locus_tag="VI935_06560"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLE25298.1"
                     /translation="MLTGGTGFIGGYLVGKLVARGDYVKCLVRKTSNVERLKSLGVEL
                     HYGDLLDFDSLLEATRGVDIVYHCAALVTDWGIRKEFYRVNVEGTRNVLKACEENSVQ
                     RVIHVSSNDVIWRFDNHIEIDEGYPYPKKYKHPYSETKAESEKLALKYNYEGRVEAVI
                     IRPCIVWGPGDKVILPRIVQLAIKGGLVLIGNGSNLISLSYIENLTDAMILASEKREA
                     RGRVYFINDGIKITFADYISRLLTSLGIELSPLRSIPYFLAYGLASVMEVWAMLLKSK
                     TPPVLTRYAVALMGRNLNYSIEKARRELNYHPRINLDEGLRQLKHWVDSIGGISALME
                     LK"
     gene            8180..10714
                     /locus_tag="VI935_06565"
     CDS             8180..10714
                     /locus_tag="VI935_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLE25299.1"
                     /translation="MSCLFNTEKFKVIALIVIGLSIVLGINSIIQGIKWTEKPFPGFL
                     LYNPVVVSNVTLIHWAQNQGLGLHSYDKIVEVDARKVSSSDEVYSIVTNKPIGTPINY
                     VIFRNNNFTRLTIPTMKFTISDFLQVYGIEFAVGLIFLVTGASVYFLKPDLISSRVFL
                     ILCSLIGIWFIEDFDYQTTYSLIYSVNISYLTQIFTPALLIFLALVFPSEKGFFKKNR
                     YIILLPLLFSSLLFILQLIFLDSPVIWKKLDTVMWFYILLGALAVLASTAISYLKPNS
                     ELDKQRAQVVLLGALLGYFLPAIAALIIVFYKISNLNYVTIPVLFFPLSVAYAIVKHK
                     LFDIDEIVRKGLTYATLTGVTVTVFGLTVVSFNWILADYGGWKHPATFMVPAIFMVLA
                     LNPLRNRIQDIIDTAFFRKSYDSSKTISELSAAMISILNIDEIANKIINTIAETMFLN
                     STSLFLFDRDTGYYRVYVTTVDDLKNKDFTLKTDDQLIFFLNRYKREIFKEDLINEQK
                     YIIYRDELMKSFQKMGASVFIPLFFKDELVGILSLGEKKSGLMYNSHDMKLLRTLANQ
                     SAIAVENAFAYKLVEDYAKKLEEANKELRETQAQLIQSEKMSAVGQLAAGIAHEIRNP
                     LNIIEGARYYLSQMISGENSRIIGEYLDYIKNEVDRTNRLIDNLLKFSRVEPQHFELV
                     DINNILEDTLSLLRKQLSDNNIRLITNFNYQIPKIMGDLNQLWQVFINILINAIQAMP
                     KDGELRIDTGLSYGSQDRIFISFKDTGIGIDEKNLPKIFNPFFTTKDIGTGLGLSISY
                     KIVEEHKGRIVVNSEKGRGSIFILELPVSYSNKAGEDDQEQKSLSS"
     gene            10683..12587
                     /locus_tag="VI935_06570"
     CDS             10683..12587
                     /locus_tag="VI935_06570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLE25300.1"
                     /translation="MTKNKRALVVDDEHMILKIISDILTKEGYEVKTAFNFDKAFELL
                     KEYPFHVVLTDIRMPEKNGIDLLEKIRTFNSNIPVILMTGFASLETAVKAVQHGAFDY
                     LTKPLDYDKLKSVTKHAFERYELLQENSRLVRELQELNASLELKVKERTRDLGNILSS
                     THESIVTMDKDLIIKSVNLKTVNIFGEGYIGRKIGDFIEGINFDSIIPKILADPSYST
                     KHEVRYGNKFLELTLSPLIDFETGNIFGVMAVTEDVTEKKKLEAQLIQSAKMSAVGQL
                     AAGIAHEFNNILTGIVGYTSFALSKTDIEQIRRDLKIVEKASDRAVEIVKKLLFFSKQ
                     KEGQFRLASIEEAIEDTLALIEHSFQSEGVKILRHYGKIPPIRMDVGEIQRVILNMAM
                     NSKHAMPQGGVIAMSTELEDDYVKIDFSDTGVGIPKEDLARIFEPFFTTKGSRGSGQT
                     PGTGLGLSVTYAIVERHGGRIDVESEVERGTTFTIRLPNIQRLSNSTKSDSLPKDDDK
                     VLQTKRKGNILIVDDEEFVCDILRQALSSVGHNVVIANNGEAAVELVRRNHFDIFFLN
                     LTMPGKNGLSVLREIKILDPSSVVVIISGRTEKDISDKAIAEGAFSFIRKPFTVSQIH
                     NTVARIFGAE"
     gene            complement(12799..>13136)
                     /locus_tag="VI935_06575"
     CDS             complement(12799..>13136)
                     /locus_tag="VI935_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018282001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLE25301.1"
                     /translation="AGYPLLSDEPVIVEDLITETRFSGPPLLHDHGVVSGISTIIYGK
                     NQPFGVLGAHTTRRQMFTKDDVHFLQAVANVIAEANERKQAEEVLKESFAKLSKKNRY
                     EAIFLPFGI"
BASE COUNT         3999 a         2376 c         2716 g         4045 t
ORIGIN      
        1 taattcatga gcaatgtggc gacgctcgat ctcttgtatc tccatcaact gatgggaaag
       61 ggtctgaagt cgctctgtat acaactgtat tttctgctcc gactccttgc gctcagtgat
      121 gtcttccaca actcccatcc ccccgataac gtttccatcg gcatcacgta gtggagaaag
      181 actcactgat aaccatagct tcgctgaact gctggtgact tcatagaaac tctcttggcg
      241 acaagactga cctttaagca ccttcttcat tgcaggcatg aaactctggt ctttcagctt
      301 ccggatatcg agcccgatga gtttgtcgta ggaagattgg agaatctcaa ccatgcgttc
      361 gttgcattgt gttatcttga agtctttatc aaacatatat accccaactg gcgattgatc
      421 aaagagggtg cggtagcgtt cctctgattg cctcagtgcc tcatcctgct gtgcattatt
      481 gatcgctacc tctatttgct gcgctacaat ctccagtagc tttaactcct cctcgtcaaa
      541 ggcatctttc ttaaaagaat ttatatttat acaccctatt gtcctgctat caaaccgaat
      601 aggcatagat gcatagcttt ttgttcccac tttccttccg gcagccccaa taaccgtatc
      661 ctgatccacg tccgcacaat acatgggttt tccttttatg attgttttcc atgtaaaacc
      721 tttaggatag cggattcttc ttacccgctc aataaaccaa tcagggtaac ccctataggt
      781 tttcataact gcttctttcc cttcgactag gtaaatacaa acattatcta caccatctat
      841 atttttactc atggattcaa cggcgttttc caggacgtcc tggagattga ttgattgatg
      901 gacactgcga gtcacagtac taatgattgt ctcgtatcta ttctttttgg ataactggac
      961 aaggttctct ctaagggcct cttctgattg tcgcagtgcc tctgcctgct tcgcattatt
     1021 tatcgctacc tcgatttgct gtgcaacaat ttccagtagg tttaactcct cttcatcgaa
     1081 ggcattcttc tgaaaagaat ttacatttat gcatcccact gtcttacctt ctaagtgaat
     1141 aggcatagat aaatagcttt ttgttcctaa ttctcttcca gcagggccga taaatgtatc
     1201 tttttccgca tctgcaacat atctgggctt cccttctatg attgccttcc atgtaaagcc
     1261 tttaggatag gggattcttc ccactcgctt aacccaccaa tcagagtagc ctctataagc
     1321 ctttagaatc gcctcttctc cttcaaccag gtaaattgaa atgttctcca ccctatctat
     1381 attcttactc atggattcaa cggcattttc taaaacctct tgtagttcta ttgaccgatg
     1441 aacaccctga gtcacagtac gaatgattgt ctcgtaacga ttctttctct ctaattgcag
     1501 cacctcgtct tccagcttct tgatcagccg cccattatat tccttgaggt aaacagcctc
     1561 ctcttcaacc ggaagttttg gtgctacaag cattcctgac tcgtggcttc tgataatctc
     1621 cctcaggatt tcgataaagg catctggctt ttgaggtttg atgatgaact tttctgcccc
     1681 cagactcaaa gcaaattctt catccttggg atcagtgtag gcggcggtat agaagacaaa
     1741 agcgagtcgt ctcaaccttt cattcgtctt aattttacgg cagagctgaa acccatccat
     1801 ccggggcatc atgatgtcag aaattatcat atcgaactcg tcctgcaacg ccttctccaa
     1861 ggcttcgagg ccatcggcag caaactcaac ctcatagcca ttgtccctga gaagtgtccc
     1921 tagcatgtaa agattctctt tgttgtcatc aacgagtaag attttcattg tccacctctc
     1981 ttacatcaag agtaaacatt caactaccag cttctgattg ctgaacgctt aagactaagc
     2041 atacttctgt gtgaatctta agtatctcta gacgtaatct ttcatccgga atgggaagct
     2101 ctaagattag gtcaaacctt tcgggacgca aaagtgcagc gtcgagcatc tcagttctat
     2161 ttgttgctgc taaaatccac acgtttttaa ttcctctatt ccattcaaag acctgaggat
     2221 gcttcagggg aagttctacc atttcccttt atcttttgaa tctcatggga gagacctccg
     2281 atgtcctcgt atgttatcct tagttttttg gctccttctt cttttctctc ttcaatctcg
     2341 ataatagtct gtggatgaat tactactaca tatacataat gaggtatggt gtttaaagct
     2401 ataaaatcct ggtagcggga accaaagaaa gcagcacgga ttctatcccc agcaaccaag
     2461 ggcaagccgt ctaggagctt tcccatgtaa tctatatccc ttttcttttt aaaggcgttg
     2521 gtttctccag ccggtgttgc tactattcgg cccgctggtc tgggaagaat cttttttatg
     2581 gagacctttt tcccaatact tacaacgaca tttccacgta taattccatc aatctgaact
     2641 atctctttac tcctcgcatc actaaaggtt ggtatgacct ttgcaacact tattttttcc
     2701 ctagagattt tttaaggttc ttgccctacc tgcttttcct ataatatagt gcaaagactg
     2761 attttataac gctactaatt tggttttttg taaagggttt tttcacaacc gaaatggaac
     2821 ccagttctaa gacttgatcc ataagctcac tatctggata gccggttata ataaccacag
     2881 gaaggtttga gtttatttcc tttatctgct ttagaacctc tactccactt atatctggca
     2941 ttgcaaggtc taagaatacc aaatcgaaag tggagatttt aatccattta atagcctcta
     3001 ccccattttg aacggctact acccgatgtc catctgagga aattatattt ttaagaaggt
     3061 cacagataac ctcatcatta tccacaacta aaaccttttt ctctttaggt atctgattcc
     3121 aaagccactt attaatcagt ccctctgtaa atctccattg attgctcact ttaaaggcag
     3181 ggattttccc ctcttctaat aatcagtata gagtcgcttt agtaatatta agatattcgg
     3241 ctgcttcttt caaggcttct ttcaagggta aatttgagta cgtgaaattt aaatagtata
     3301 ataagttaaa ctgatttaat aaaggagggt atagagataa agtttctatt aagagtagca
     3361 attattgcat tgcttctttt accgcttctc agtgtaagtt ccaaggcggt aagccagcaa
     3421 gaacaaatag aagcgctcaa aaaacagatt gaggaaatcg ggagacaaaa ccagaaacaa
     3481 attgaagagc ttcagaggca gaaccaagag caaattgaac agcttcagag aaagatcgaa
     3541 gagttagagg ccgaaaggaa aactgagcaa aaaaagattg aggaggtagt gaccaagaaa
     3601 gaggctgaag agaaggaagc ctggtggaaa aagttaaggt taataggatt taatcctgat
     3661 tttcccagct attttcggat tcggtcaatg ttctttaaga aagccacatt tgtaggcgca
     3721 gttccaggta aagatgatga catattcttt gttgattcaa gattgcttat atctccaatg
     3781 cttaaaatta ctgacaattt gtcaattcgt gctcagatag atgtttttaa aaatataatt
     3841 tggggtggct taggtgatgc aagtgtagcc gataagttct tcgaagcccc aagccctgct
     3901 gattcatttc gtggagctct gcttagggaa gtaatagata caatttcagg gaacgttctt
     3961 tcaccagttg aagaggtaga tttttttgat attagatcac tttacctggt tgccagagta
     4021 ccaataggag aactatgggt tggacgacag ccgtatgatt ggggactcgg agttttagct
     4081 aatgctggaa gtatgcctga tcaggacctt ggaagtatta tagatagatt tgaatttgat
     4141 actgcgcctc ttgctcttct tgatgaacgc tgggaaaatt taactttcgc attcttaatt
     4201 gatagattga gtcaaggtat gagtttagtt aatggagcta gtgggagtgg atgggaggta
     4261 ggatttggcg ctttatatcg tgttgagaat ctgcaactcg gaggttacat atatcttctt
     4321 tctcagaaca attttgatat tagtggtgga cttactggag atctcgataa caatgtgaat
     4381 tggtcaatat acgctaatta taattatgat cctttcttct tctctctcga attccaagac
     4441 ttgtttggaa aagtaaatga tttagaagaa cctcttccct caattttagg aaataatgac
     4501 attgatatat cgcctgagaa ttttcttatt gctgctagag ttggatatga tccaccacca
     4561 gcttttatag actttattgt tgctgaattt ggctgggcta atggtaatga tgcaaaaact
     4621 ccggacaaat tagaaggcaa tgcgattttt tttaataatg cttatacagt tgataattta
     4681 ctattcaagc atattatacc gaatatttat gcacttgagg ggagtgtaat aaattctgga
     4741 tacgtaagag catggagcac tttgaaatta aatgactcgt tttattttac accacaagcc
     4801 ctctttgcct gggtggatga gagaaatgct ctaagtcttg atcttcttac tccccttcct
     4861 aaggttggta gatttctagg agctgagata gagggtactc ttacttggag gattatagat
     4921 catctttggt ttgacctaat aggtagtgtt gtaatagctg gtgatggtct caaggacctt
     4981 ttcagccaaa gagcttttat tgaaggagta gtcccatcaa tagggacagc cagacctcca
     5041 gacgcacctt ttgcttttca gggaagattt gtggttacca tagattctct tattaagaca
     5101 tggactggtt ctagtagcct tttaaagaga gcatattatt actagaagat atagaagctt
     5161 taggacccaa ctatcagagg tagcttttga cattataagc taattaaaaa tcaatgattg
     5221 cagtctatta cgttacaaaa ctaaaaactc tcctttactg tttagttata agatacctta
     5281 gtttaagtaa cttagtaaaa ttcctaagac ttaagttttg agtaatagaa agtcttgaat
     5341 tagctctggt tactttgtac aataagaatt ctaagaccca atagaacttg aaaattgaat
     5401 atgcaagaca aatagttgag agctttttag ttagagcgtt atctaaaatg gacgattagg
     5461 atctagaagg tagagaatac aattcatgaa gaggaagatg taaatgccga taactttaga
     5521 acgcagaaag tgcttaagaa cgcccttagt aggtctcgag attttctcaa gaaatactgg
     5581 ggaactaatc ggaatgtcaa gggatattac tccttatggg atgtttattg aaaccaaaaa
     5641 tctactagtg cctggtatta agatactcct cgaattcaaa ttgcctggtc aagtaatacc
     5701 tataaaagcc tacagcgagg ttaaatgggt tagaaggagc acgactttag aagctggacc
     5761 ttccggaatg ggagctcact tcttaggtat ttatgagtca gataaacagc ggttagcaaa
     5821 atatagtgaa agcttggcta agcaagaaaa aataaactct gataatttta ctctagttga
     5881 tttcttgaag gtttcaggtg acgacttatt tatgaagaca aagcctgttt ggggatacat
     5941 agaggatatg aaacaaaagg gtttttatac ctatagaaga ccgttacttt cagcttgcag
     6001 aaatagagtg gtgatttttg atgaaacaac aggtaaagaa agggaaatga ttatgatggg
     6061 tagtaataac tatctcggtc ttactgccca tccaaaggta ataagagcag cagaagaagc
     6121 aatagaaaag tatggtacgg gtgctggctc agttcctctc cttgctggta catactactt
     6181 acataaagag ttagaagcta agttagcaaa gttaaaagga tgcgaagatg cggtgatttt
     6241 tccaaccgga tattcaacaa acgtgggttg tctatctgct ttggttaaag agaaagatct
     6301 tgcagtgctt gatagactta cccatgcaag cattatagat ggttgcatga tgagtagtgg
     6361 aaaatttaga actttccagc attcagatat gcaaaaccta agagaggtgc tcgaaagaaa
     6421 taaagatagt tttgatggaa aacttatagc ggttgatggt gtatacagca tggatggtga
     6481 tgcagccccc ctccctgaga ttgttgatat tgcccaagaa tacggggcaa gagtaatggt
     6541 agacgatgca catgcaactg gagtaattgg agaaagagga aggggtactg caagtcattt
     6601 taagatggtg ggcaaaatag atatagtaat gggtacacta agtaaagctt tgggtcaatt
     6661 aggtggcttt gttgcttctt ctaaggaggt tgttaattac ctaagatgtt ttgcaaggtc
     6721 atactttttt tcgtcaagtc ttccacctgt tgtggtagct tcggttttag ctgcaattga
     6781 ggtaatggaa agtgagccgg agcttcatca aaacctttgg aaaaacatca aatactttaa
     6841 ggagaaccta cagtatctag ggtttaatat taggaattct gagtcagcta taattcccat
     6901 tattattggt gatgaacttc ttcttagaaa aatgagtaag agaatacacg aagagggtat
     6961 ttacataaat cctattcctt atccggctgt tccaaggaat caaacccggt ttagagcaag
     7021 cattatggca acccatacta gagaggattt agatactact ttagaagttc tagaaaaggt
     7081 tggaagagaa tttggtgtca taaaaaaagg ccttgcaatc ttttcaagaa aagcagctta
     7141 gtgtaaagct ttaaggctat accgtgaaag taatgttaac tggagggaca gggtttatag
     7201 gtggatatct ggtaggcaaa ctggtagcga ggggagatta tgttaaatgc ttagtcagaa
     7261 aaacgagtaa cgttgaaaga ctaaaaagcc taggagttga gcttcattac ggggatttat
     7321 tagattttga ttctctttta gaagctacca ggggagttga tatagtatat cactgtgcag
     7381 cccttgttac agattgggga attcgtaagg aattctatag ggttaatgta gaaggaacaa
     7441 ggaatgtact taaggcatgt gaagaaaata gtgtgcagcg agttatccat gtaagtagca
     7501 atgacgttat ttggagattt gataatcata ttgaaataga tgaaggttat ccttatccaa
     7561 aaaagtataa gcatccctac agtgagacaa aagctgaatc agaaaaatta gcccttaagt
     7621 ataattacga aggaagggta gaagcggtta tcatacggcc atgcatcgta tggggaccgg
     7681 gtgataaagt gattcttcct agaatcgtac agttagcaat aaagggaggc ctagttttaa
     7741 taggtaatgg aagtaatctt atttctttga gttatatcga aaacttaact gatgctatga
     7801 tactagcaag tgagaagagg gaagctagag gaagagttta ttttataaat gatggcatta
     7861 aaattacttt tgcagattat atatcacgcc ttcttacctc tcttgggata gaattgtcac
     7921 ctctgagatc cattccctac ttcttagctt atggactagc ctcggtgatg gaggtatggg
     7981 caatgttatt aaagtccaaa actcctccag ttttaaccag gtacgctgtt gctttaatgg
     8041 gtcgaaattt aaattatagc attgaaaagg ctaggagaga attaaattac catcctagaa
     8101 ttaaccttga cgaaggacta agacagttaa aacattgggt tgatagtatt ggtggtatta
     8161 gtgctcttat ggaattaaaa tgagttgctt atttaatacc gaaaaattta aagttatagc
     8221 ccttatagta attggtttat ctattgttct tggtataaat agtataattc aaggaataaa
     8281 gtggacagag aaacctttcc ccggtttttt actttataat cctgtagtag ttagtaatgt
     8341 cactcttatc cattgggcac agaaccaggg actcggttta cattcttacg ataagatagt
     8401 agaggtagat gcaaggaaag tatcaagctc ggatgaagtc tatagtattg tcactaataa
     8461 acccattggc actcctataa attatgtaat ctttagaaac aataatttca ctaggctgac
     8521 tattcctact atgaaattta ccattagtga ttttctacag gtatatggta tagaatttgc
     8581 tgttggttta atttttcttg ttacaggagc ttcggtttat tttttaaaac cagatttaat
     8641 ttctagtaga gtattcttaa tactttgttc tttaatcggg atttggttta tagaggactt
     8701 tgattatcaa accacatatt cacttatata ttcagttaat atctcgtatc taacccagat
     8761 ttttactcct gctcttttaa tttttttagc attagttttt cctagtgaaa agggattttt
     8821 taaaaagaat cgctatatta ttttacttcc cttattattt tcgtcactac tatttatcct
     8881 acaactaatc tttcttgatt ccccagttat ttggaaaaaa ttagacacag taatgtggtt
     8941 ttatatattg ttaggtgcct tagcagtgtt ggcttctaca gcgataagct atttaaaacc
     9001 taattctgag ttagataaac aaagggctca agttgtgtta ttaggagcac ttttagggta
     9061 tttcttacct gcaatagccg cattgataat tgttttttat aaaatcagta accttaatta
     9121 tgtaactatt cctgtcttat ttttccccct ctcagtagca tacgccattg taaagcataa
     9181 gctctttgat atagatgaaa ttgttagaaa aggcttgact tatgccactc tcacaggagt
     9241 gacagttact gtatttggtc taacagtagt tagtttcaat tggattcttg cagattacgg
     9301 gggctggaag catccagcga cttttatggt ccctgctatt ttcatggttc tcgctttgaa
     9361 tcctcttagg aatcgaattc aggatataat agatacagcc ttttttagaa aaagctatga
     9421 ctccagtaaa acaatatcag agctaagcgc tgctatgatc tcgatactga atattgacga
     9481 aatagcaaat aaaattatta atactatagc agaaactatg ttccttaact caacgtcact
     9541 tttcctcttc gatcgtgata ccggttatta ccgagtttat gtgactacgg tagatgacct
     9601 gaagaataag gattttactc ttaaaactga tgaccaatta atcttctttt taaatagata
     9661 caaaagagag atttttaaag aagatttaat aaatgagcaa aaatatatta tatataggga
     9721 tgaattaatg aaatccttcc agaaaatggg ggcttctgtc ttcattccac ttttctttaa
     9781 agatgagtta gttggtatac tgtcgttggg tgagaagaaa tctggtctca tgtataatag
     9841 ccatgatatg aagcttctta gaacactcgc taatcagagt gcgattgctg ttgaaaatgc
     9901 ctttgcttat aaattagttg aggattacgc gaaaaaattg gaggaggcta ataaagagct
     9961 tagagaaact caggcacagc ttattcaatc agaaaagatg tcggcagtag gacagcttgc
    10021 agcagggatt gcacacgaga ttcgtaatcc actgaatatt atagagggtg caagatatta
    10081 tttgtctcaa atgataagcg gagaaaattc taggattata ggagagtatc tggattacat
    10141 caaaaacgag gttgatcgaa caaaccgttt gattgataac ctgcttaaat tctcgagagt
    10201 ggaaccacag cattttgagc tagtagatat aaacaatatc cttgaggaca ctctgagtct
    10261 cttgagaaag cagctatctg ataataatat cagacttata acaaacttta actaccagat
    10321 tccaaaaatt atgggtgatc taaatcaact ctggcaggtc tttattaaca ttctaataaa
    10381 tgctattcag gcaatgccta aagatggaga actccgaata gatacggggc tttcttacgg
    10441 aagccaagac cgcatattta taagctttaa agatacaggc atagggatag acgagaagaa
    10501 tcttccaaag atttttaatc cctttttcac aacgaaagac ataggaacag ggcttggtct
    10561 ttctataagc tataaaatag ttgaagagca taagggaaga attgtagtaa acagtgagaa
    10621 gggaagaggc agcatcttta ttttagaact tcctgttagt tacagtaata aggcaggaga
    10681 ggatgaccaa gaacaaaaga gccttagtag ttgacgacga gcatatgatt ctaaaaataa
    10741 tctcagacat cctcacaaaa gagggttatg aggtaaagac tgcttttaat ttcgacaagg
    10801 catttgagct cctaaaggaa tatcccttcc atgttgttct taccgatatc cgaatgcccg
    10861 aaaaaaatgg aatagacctt ctagagaaaa tccggacttt taattcaaat atacctgtca
    10921 ttcttatgac ggggtttgct tctcttgaaa ccgcagtaaa agcagtccag catggagcat
    10981 ttgattatct cacaaaaccc ctagactatg ataagctaaa aagtgtaaca aaacatgcat
    11041 ttgagagata tgagcttctg caagaaaaca gtcggcttgt acgggaactt caggaactaa
    11101 atgcgagtct tgaactcaag gttaaagaaa gaactcgaga tcttggaaat atactaagca
    11161 gcactcacga aagtatagta actatggata aagacctgat tataaaaagt gtaaacctaa
    11221 aaactgtgaa tatctttggt gagggctata ttggacgaaa aatcggtgat tttatagaag
    11281 gcattaactt tgactctatt attcccaaaa tactggctga cccctcttac agcacaaagc
    11341 acgaggtaag atatggaaat aaatttttag agcttactct ttctcctctt attgattttg
    11401 aaacaggcaa catatttggt gtgatggctg ttactgaaga tgtcacagag aaaaagaaat
    11461 tggaggcaca actgattcaa tctgctaaaa tgtctgctgt agggcagctt gcagcaggga
    11521 ttgcacatga gtttaataat attctcacag gaattgtggg ttatacgagc tttgccttgt
    11581 caaaaacgga catcgaacag attaggagag acttgaaaat agtggaaaag gcttcggata
    11641 gagcagtaga gatcgtgaag aagttgcttt ttttctcaaa acaaaaggaa gggcagtttc
    11701 gtcttgcctc gatagaggaa gcgatagaag atacactggc tctcatagag cattcttttc
    11761 aatcagaggg ggtgaagatt ctacgtcatt acggaaagat tcctcctatt agaatggatg
    11821 tgggtgagat tcagcgggtt atactcaata tggcgatgaa ttcaaagcat gcaatgcccc
    11881 aaggcggtgt gattgctatg agcaccgagt tggaggacga ttatgtgaag atagattttt
    11941 ctgataccgg ggttggaatt ccaaaagagg atctagcaag gatttttgag ccgtttttta
    12001 caacaaaagg aagtcgcggt agcggtcaaa cgcctggcac tggacttgga ctctctgtga
    12061 cttatgcaat agttgagagg cacgggggac gaatagatgt tgagagtgag gttgaacgtg
    12121 ggacaacatt tacaatacgg cttccaaata ttcagcgact ctcaaattcg accaaaagcg
    12181 attcactacc caaggacgat gataaagtct tacaaacaaa aagaaaggga aatattctta
    12241 tagtagacga tgaagagttt gtatgtgata tacttagaca agccctttca agcgttggtc
    12301 ataatgttgt tatagcaaat aacggtgaag ccgctgtaga gcttgtaaga agaaaccact
    12361 ttgatatttt ctttcttaac ttaacgatgc ctggaaagaa cggtcttagt gtgcttaggg
    12421 aaataaagat tttagaccca agttcagtcg ttgtaattat aagtggaaga acagaaaaag
    12481 atatatcgga taaagcaata gccgaaggcg cattttcttt tatacgcaaa ccttttacag
    12541 tgtctcagat ccataatact gtagcacgta tttttggggc tgagtgagaa ggaaagacct
    12601 gaatctgcca tttcgaagca tcttcagagc cttgttccat gaagttatta ttagaaagga
    12661 caagtcaaag ttcactatta ggtgtctaat accatttccg gatgaaaagt atttgtataa
    12721 gaaagggtct ggtataaact ttctatatgc cgaaacgcat aagtattgaa ccaaatttac
    12781 gcttgacaga gctggaagct agataccgaa aggcaaaaat attgcttcgt aacgattctt
    12841 tttggataac ttggcgaagc tctccttaag cacctcctcc gcctgcttgc gctcattcgc
    12901 ctcagcaatc acattagcga cggcctggag aaagtgaacg tcgtccttgg taaacatttg
    12961 tcgtctggtc gtgtgtgcac ctaagacgcc aaaaggctgg ttcttgccgt agatgatggt
    13021 gcttatgccg ctaaccaccc cgtggtcgtg aagtagcggt ggcccgctga accgcgtttc
    13081 cgtgattagg tcctctacaa tcaccggctc atcggaaagc agggggtagc ctgcct
//
LOCUS       DATDRL010000191         8240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3230235, whole genome shotgun sequence.
ACCESSION   DATDRL010000191 DATDRL010000000
VERSION     DATDRL010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8240
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3230235"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VI935_06580"
     CDS             complement(<1..154)
                     /locus_tag="VI935_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLE25302.1"
                     /translation="MSNNITIEKTRNIGIIAHIDAGKTTTTERILYYTGLTYKIGEVH
                     EGTAIMD"
     gene            complement(173..643)
                     /gene="rpsG"
                     /locus_tag="VI935_06585"
     CDS             complement(173..643)
                     /gene="rpsG"
                     /locus_tag="VI935_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HLE25303.1"
                     /translation="MSRKGSVSKRRISTDPKYGDLMAAKFINSLMYDGKKSIAENIFY
                     KAVETVGKKMEKDPLEVFRTAMENVKPVIEVRPRRVGGATYQVPIEVNQFRRLSLAIR
                     WVINAARGRQGKSMEEKLASEIMDAAQGRGGAIKKREDVQKMAEANRAFAHYRW"
     gene            complement(660..1031)
                     /gene="rpsL"
                     /locus_tag="VI935_06590"
     CDS             complement(660..1031)
                     /gene="rpsL"
                     /locus_tag="VI935_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HLE25304.1"
                     /translation="MPTIGQLVKEGREQLTKKSKAPALQNCPQKRGVCVRVYTTTPKK
                     PNSALRKVARVRLTNGIEITGYIPGEGHTLQEHSVVLVRGGRVKDLPGVRYHIIRGSL
                     DATGVQNRRKSRSKYGAKRPK"
     gene            1187..1438
                     /locus_tag="VI935_06595"
     CDS             1187..1438
                     /locus_tag="VI935_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25305.1"
                     /translation="MAQLNKKFTDSQIKELRERYVRNEIERKYIQQVLGISKTRFFAL
                     IKLYRKNPNKFSVRNSIDITTAKFIQQLGRFLILGFRER"
     gene            complement(1764..1934)
                     /locus_tag="VI935_06600"
     CDS             complement(1764..1934)
                     /locus_tag="VI935_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25306.1"
                     /translation="MLQGIVIDDSKLFNEKLREWENFYNFQRPHGSLSGQTPFERLQE
                     RLKELSLTEVTL"
     gene            complement(2354..2602)
                     /locus_tag="VI935_06605"
     CDS             complement(2354..2602)
                     /locus_tag="VI935_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine zipper domain-containing protein"
                     /protein_id="HLE25307.1"
                     /translation="MARKTYYFWWNRYQEYGFEGVKVRSRKPLHSPRATKTKIIEKVI
                     YLRQHYYFGPLKIQMYLLRYHDIRITPPLFIGFLKDYK"
     gene            complement(2539..2709)
                     /locus_tag="VI935_06610"
     CDS             complement(2539..2709)
                     /locus_tag="VI935_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25308.1"
                     /translation="MTQEQYQQKLANLRLSWFHHVQEVTHKVAKHVAIMGLLEKRTTS
                     GGTGIRNMVLKG"
     gene            2788..2907
                     /locus_tag="VI935_06615"
                     /pseudo
     CDS             2788..2907
                     /locus_tag="VI935_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
     gene            complement(2904..3857)
                     /locus_tag="VI935_06620"
     CDS             complement(2904..3857)
                     /locus_tag="VI935_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HLE25309.1"
                     /translation="MKQLEPFATESLASFYGRIDTNWQPTDFCPRSEDKDFAREIKEI
                     QERAKALSYEVLAVLIGNMVTEEGLPTYESAFFPVEGIHHLEADGWSRWVRSWTAEEN
                     RHGDVLNRYLYLCGRVDMKQLEITIQYILADGFNTQTMDDPYRSFIYTSFQERATNIS
                     HRRTSTLAKKAGDGVLAKICGVVASDEARHELAFKNFVAKFFELDPDETMLAFEDMMR
                     KKIAMPAHYLREHGQEKGSTFTHFAEAAQRIGVYTAFDYAEIIEALISFWNIKSIKGL
                     KPEAEKARDYICSLPARYKKLAERFKPAQRPHKYKWILEYA"
     gene            complement(3896..4849)
                     /gene="trxB"
                     /locus_tag="VI935_06625"
     CDS             complement(3896..4849)
                     /gene="trxB"
                     /locus_tag="VI935_06625"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HLE25310.1"
                     /translation="MSNSGELKEKYKVVIIGSGPAGLTAALYAARANLEPVVFEGVQA
                     GGQLTLTTAVENYPGFPDGIMGPDLMDEMRKQAERFGAKCVLAEVTSVDLSKRPFTVV
                     AGEALLKVETLIIASGASAKLLGLDSERRLLGHGVSTCATCDGFFFKDKEVVVVGGGD
                     TAVEEAVFLTKYATKVTIIHRRDKLRASKIMQERAFKNKKIGFIWDSAVVDIISDKDK
                     EVTGVKISNVNTGEITEKACQGMFVAIGHTPNTRLFEGQLEMDERGYIITKNSTTETN
                     VPGVFAAGDVQDQRYRQAVTAAGTGCMAAMDAEKFLEEHGE"
     gene            complement(5017..5877)
                     /locus_tag="VI935_06630"
     CDS             complement(5017..5877)
                     /locus_tag="VI935_06630"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HLE25311.1"
                     /translation="MIKLKFIHFFLIVLFLCFPSPVRAGWIIEQVIYSIAPSSKESSK
                     QKIKSTFYFSKNRVKSLQGNSEITINYNSNQIFIVDSGRKLYWGGTIDEYINEIARSV
                     AQSRKKLEESLKNMPPEQRKAIEEEMKKQGLRLPGEAKRSDMKVQVRIEKTPDKQTMA
                     GYDATKYKIYTDGAVYQEIWVSDEVSVYKHIDMKKMNDFQNRVNETLSSLYTSGGGGL
                     ESSLEYQKLLEKGYPLKTVQVVGQNKGIIEVVNAKETEIGNSEFEVMKGFRKTTLEEV
                     MKTNVGKETR"
     gene            6078..7169
                     /locus_tag="VI935_06635"
     CDS             6078..7169
                     /locus_tag="VI935_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HLE25312.1"
                     /translation="MKKTLLYDVHSLLGAIFDDFSGFLMAKSYNNPLEEYLAIRKGVG
                     LIDLSHHGKLRMSGKEHLKFLQGMVTNDVMKLEEGKGLYATLLTVKGKMVADMRIYRE
                     KESVLLELEPGLNEKVRELLLKYKLSYKANIEDLTDSYCLLSIQGSNSKRLLEKSLGE
                     EIPELKEYEFLKRDIVGTEVMITRVNRTGEEGYDFFIQPDGVKLLWESLIGNGRDLEL
                     KPVGLETMETLRIEAGIPRYDADMDEETIPLEAGLLDAISFEKGCYVGQEVIARIKWR
                     GHVNWCLVGFEIEGETLPSKGDKIFLGEREIGHITSSVFSPVLNRVIALGYIRREFKD
                     PETKVIVRINSESKSATVVRTPFLQSRGS"
     gene            complement(7201..7275)
                     /locus_tag="VI935_06640"
     tRNA            complement(7201..7275)
                     /locus_tag="VI935_06640"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7241..7243),aa:Val,seq:cac)
     gene            complement(7342..7572)
                     /locus_tag="VI935_06645"
     CDS             complement(7342..7572)
                     /locus_tag="VI935_06645"
                     /inference="COORDINATES: protein motif:HMM:NF016718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PUR family DNA/RNA-binding protein"
                     /protein_id="HLE25313.1"
                     /translation="MEKLEKEHLSKHVDIESKRFFFDVKENHKGKYLRITELSGGRSC
                     IVIPLGGIKLFRERLGEIIEEAEKLLIVEDDF"
     gene            7863..>8240
                     /locus_tag="VI935_06650"
     CDS             7863..>8240
                     /locus_tag="VI935_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLE25314.1"
                     /translation="MEKKLKFGIAGPVPGESVANLINYSMLYERGGFDTVWFPDHVVF
                     MAKTLTPEVWSVITAACLKTKKILMGTIGDAHRIHPAIFAHRLATVDHISSGRIFVCV
                     GYGEKMNLDPYGIRWDKPLKRVVE"
BASE COUNT         2414 a         1739 c         1493 g         2594 t
ORIGIN      
        1 agtccattat ggctgtgcct tcatgaacct cgccaatctt atatgtcaga ccagtgtaat
       61 aaaggatgcg ctctgtcgtt gtggtcttac cggcatctat atgggcaata atcccaatat
      121 ttctagtctt ttctatagtt atgttattac tcacttactc tcccctcgaa acttaccacc
      181 tataatgggc aaaggccctg ttggcttcag ccatcttttg tacatcttct ctttttttaa
      241 tcgctccccc acgcccttga gccgcatcca taatctcgga tgcaagcttt tcttccattg
      301 attttccttg cctgcccctt gcagcattga ttacccatct tatagcaaga gatagacgac
      361 gaaattgatt tacctcgatt ggaacttgat atgtagctcc acctaccctt cggggtctta
      421 cctcaataac cggctttacg ttttccatcg ctgtacgaaa aacctctagg ggatcctttt
      481 ccattttttt tccaaccgtt tcgacagctt tataaaaaat gttttcagct atgcttttct
      541 ttccatcgta cataagggaa tttataaact ttgctgccat caaatctccg tacttaggat
      601 ccgtagaaat ccgtctctta gaaactgaac cttttcttga cattgtaact tttccttaat
      661 tacttaggtc tcttggcacc atacttcgag cggctttttc ttctattttg cacgcccgta
      721 gcatccaatg agcctctgat aatatggtat cttactcctg gaaggtcttt taccctacca
      781 ccccttacta gaactacaga gtgttcctga agtgtgtgtc cttcaccagg tatataccca
      841 gttatctcaa ttccattagt aagccttaca cgtgcaacct ttctaagtgc cgagttcggt
      901 tttttgggag tagtagtata cactctgaca catacacctc ttttttgagg acagttttgt
      961 agagctggag ccttactctt cttcgttaac tgctctctcc cctctttcac tagttgccca
     1021 attgtaggca tttactatat tttctcctaa gcattaaaaa ttaatctaaa attataatca
     1081 tgaaatactt atgttgtcaa ggaatatgca cctccgtgtc atttaaaaat taaaagtgca
     1141 cacctaaata gacataatct aaaacccaaa aaggggattt tagattatgg cacagctaaa
     1201 taagaaattc acagatagtc agattaaaga acttcgtgaa cggtatgtga gaaatgagat
     1261 tgaaagaaaa tatatacaac aggttcttgg tattagcaaa acaagatttt ttgctttaat
     1321 aaaactttac cgtaaaaatc ctaataagtt ctcagtgagg aactctatag atataactac
     1381 cgccaagttc attcaacaac tggggaggtt ccttatttta ggtttcagag agcgttagaa
     1441 gagaaaaagt ctttatttag agaattcaag ataaagcctc cttatgaatc tgctaaggac
     1501 atcttttgtc taagaataga tagaactata gacgcttaca gaagaatctc aatcaatagc
     1561 ttagtgctta aagttaacgg ggctgttcca agagagactg taaatcttcg tatttatccc
     1621 atgagtaatg gaatatctga ggtaagattt tggtgcaatg ataaacttat tgatgtcaaa
     1681 agggttaaaa atagtgagtt aaatatagtt cacttttaaa ttttagctaa cagaaaatgt
     1741 acgttcgtaa gacaccagta acatcataga gtaacctcgg ttaaagataa ttctttcagt
     1801 ctttcttgta acctttcaaa aggggtttga ccagaaagag acccatgtgg cctttgaaaa
     1861 ttatagaaat tttcccattc ccttagtttt tcattaaaga gctttgaatc atcaatgaca
     1921 attccttgaa gcatttagta gaactcctcc tcgtcgattc gatacgaacg ttccactttc
     1981 ccgtttagcc ttggagtacg aggtttaatg tatctatgct caatcccttt atccagaatg
     2041 tgccagtgaa attgtggtcc aaactcagtt ccattgtcag tttggattga atgaacagag
     2101 aatggaagtt taactaggac ataatctaca aattcaatag aattcttttg attgtacctg
     2161 ttgtaaatct taagaaccct gattctagta caatcatcaa ttgcggtcta ttgataaaat
     2221 ttctttggtg ttaatccagg aatcggatcc aagaatttca catctatttg aatgttagga
     2281 ccaggaatgg gtttctcgta tcttttgaat ctttccttga ggggacgata acgctgatta
     2341 gctggcagac gattcatttg taatctttta agaatccgat aaatagcgga ggggtaattc
     2401 taatatcatg ataccgtaaa agatacatct ggatttttaa tggaccgaaa tagtaatgtt
     2461 gtcttagata aataaccttc tcaatgatct tcgttttagt agccctaggg ctgtgaagag
     2521 gcttgcgaga gcgaactttc accccttcaa aaccatattc ctgataccgg ttccaccaga
     2581 agtagtacgt ttttcgagca atcccataat agcaacatgt ttagctacct tatgagtaac
     2641 ttcttggacg tgatggaacc aagaaagcct taaattagct aatttttgtt gatattgttc
     2701 ttgagtcata gaagcatacc tccacgcatc ctcagtatac tccttctgtt acagatgtct
     2761 ggtagtccta taaggaaaca tacttaagct tttgagaaaa ctattcagca aaagttcgtc
     2821 acttacgatc tggcaagaca gattgaagga gcaaaggaag tgagttgctc ggggtttggg
     2881 gaagcgatta ttaaaaatct ctcctaagca tactccaaaa tccacttgta cttgtgcggc
     2941 cgctgggcag gtttgaagcg ctcggcgagt tttttgtagc gggccggcag gctgcaaata
     3001 tagtcgcggg ccttctcagc ttctggcttg agtcctttta tcgatttaat attccaaaaa
     3061 gaaatcagcg cctcgataat ctcggcataa tcaaaagcgg tataaacccc gatgcgctgg
     3121 gctgcttcgg caaagtgggt aaaggtggat cctttttcct ggccatgttc ccgcaaataa
     3181 tgcgccggca tcgcgatttt tttacgcatc atatcttcaa aggctaacat cgtttcgtca
     3241 ggatccaatt caaaaaactt cgcaacaaag tttttaaaag ctaactcatg ccgagcttca
     3301 tcggaggcaa ccacgccgca aattttggct aaaactccat cgccggcctt tttcgccaac
     3361 gttgatgtgc gccgatgaga gatattcgta gcacgctcct ggaagctggt ataaataaaa
     3421 ctgcgataag ggtcatccat cgtctgagta ttgaaaccat ctgccaaaat atactgaatt
     3481 gtgatctcca gctgtttcat atccacgcgg ccgcaaagat aaagatagcg attcagcaca
     3541 tcgccatggc gattctcttc cgcagtccag gaacgcaccc agcggctcca accgtcggcc
     3601 tccagatgat gaatgccttc taccgggaaa aaagctgatt cataggtcgg caagccttct
     3661 tcagtcacca tattccctat caagaccgct agaacttcgt aagacaaagc tttggctctc
     3721 tcctgaattt ctttgatctc tctggcaaaa tccttatctt cacttctggg gcaaaagtct
     3781 gtcggctgcc aattggtatc aatgcggcca taaaagctcg ccagagactc cgtggcaaaa
     3841 ggctcgagct gtttcataac ttcttcacgg tgaggataca caatcaaaat tttaattatt
     3901 ccccgtgttc ttcaaggaac ttttctgcat ccatagcagc catgcaccct gtgccagccg
     3961 ctgttacagc ctgacgatac ctttggtctt gaacatcccc agcagcaaac accccaggaa
     4021 cattcgtttc ggttgtactg ttttttgtaa tgatataacc cctttcatcc atctctagtt
     4081 gtccctcaaa aagcctcgta tttggtgtat gaccaatagc aacaaacatc ccttgacatg
     4141 ctttttcggt aatctcccca gtatttacat tacttatttt tactccagtt acttctttgt
     4201 ctttatcact tataatatct acaacagcag agtcccaaat aaatcctatc tttttgtttt
     4261 taaatgccct ctcttgcata atctttgacg ctcttagctt atcccttcta tgaataatag
     4321 tgaccttcgt tgcatacttt gtaagaaata ccgcctcctc tactgctgtg tcgccaccgc
     4381 caactacaac tacttcctta tctttgaaga aaaaaccatc acacgtagcg caggtagaga
     4441 cgccatgtcc aagaagcctt ctttcagaat caagtccaag gagctttgct gaagctccgg
     4501 aagctataat caatgtttct acttttaata aagcctctcc agctactacc gtgaacggcc
     4561 tttttgacaa gtctacagaa gttacctctg ccaaaacaca cttcgcaccg aatctttctg
     4621 cttgttttct catttcatcc atcaggtcag gacccataat cccatctgga aaccctggat
     4681 agttttcaac agcggttgtc aatgtaagct gaccacctgc ttgtacccct tcaaaaacaa
     4741 ctggctcgag gtttgccctt gctgcgtata atgctgctgt gagtccagcg ggtccggaac
     4801 caatgattac aactttgtat ttttctttca actcgcctga attactcatc tctcacatct
     4861 tctaaattgt ttcttattta tataacattc ggtaatgtag cattcaagta gtatgattct
     4921 taaattctca gcaagatata ttgataaacc tttttatttt agatgcaaga cctcaaaaat
     4981 ggataccaaa ttctgaatca tttcatatca aattcatcat cttgtttcct tgcctacatt
     5041 tgttttcatt acttcttcaa gagttgtctt tctaaaaccc ttcattacct caaactcgct
     5101 atttccgatt tctgtttcct tagcattaac tacctctata attcctttat tctgcccgac
     5161 aacttgaact gtttttagag gatacccctt ttctaataac ttttgatatt ccaagctaga
     5221 ctctaagcct ccaccaccac ttgtgtatag tgaactcaga gtttcgttaa ccctattttg
     5281 gaagtcattc atctttttca tgtcaatatg tttatataca ctcacctcat ctgaaaccca
     5341 aatctcttga taaacagccc catcggtata aatcttatac ttggtagcat catatcctgc
     5401 catagtttgt ttatcggggg tcttttcaat tctcacctgt actttcatgt ccgaccgctt
     5461 agcctccccg ggaagtcgta gtccctgttt tttcatctcc tcctcaatag cttttctctg
     5521 ctctggcggc atgtttttta gagattcttc caatttcttt cttgactgag caacggacct
     5581 cgctatctca tttatatact catcaattgt gccaccccag taaagctttc taccggaatc
     5641 aacaatgaat atctgattgc tattataatt tatggttatc tctgagttac cctgaaggga
     5701 tttaaccctg tttttagaga aataaaacgt ggatttgatt ttttgcttcg aagattcctt
     5761 ggaagaaggc gctatgctgt aaataacctg ttcgattatc catcccgctc ttacaggact
     5821 ggggaaacat aagaaaagca cgataagaaa aaagtgtata aatttaagct taatcatagt
     5881 tgctcccttt taaagattga atctaaatca taatatatta tcatcgccat attcaaaaaa
     5941 ctcaatttac cgttaacaaa aaagcttgtt ataattattt tgattgggat accataaata
     6001 ttatatcgta atatttatta ctgagagaat cgagcgttat tataatttta ccctaaaaca
     6061 atttttggaa agttaaaatg aaaaaaacct tgctttatga tgttcattct ttgctaggtg
     6121 ccatttttga tgacttttca ggttttttaa tggctaaaag ttacaataac cctctagagg
     6181 aatatctcgc tataagaaaa ggcgttggac ttattgatct ttcgcaccat gggaagttgc
     6241 gtatgagtgg aaaggaacat ttaaaattcc tacagggcat ggtaactaat gatgttatga
     6301 aattagaaga aggaaaaggc ctatacgcaa ccctacttac agtaaaggga aaaatggtcg
     6361 cagatatgag gatatatcgg gagaaagaat ctgtactact cgagctagag ccgggtctta
     6421 atgaaaaagt aagagaactt ttgcttaaat ataaactatc ctacaaagca aatattgaag
     6481 acctaaccga tagttactgt ttactctcta ttcaaggttc taattccaaa aggcttcttg
     6541 aaaaatcctt gggtgaggaa atcccagaac taaaggaata tgaatttctt aaaagagata
     6601 ttgtcggcac tgaggttatg ataacaagag taaatcgaac aggggaagag ggatatgatt
     6661 ttttcatcca acctgatgga gttaagcttc tttgggaatc tctaattgga aatggcagag
     6721 accttgaatt aaagcctgtt ggtcttgaga caatggaaac cttaagaatt gaagcaggca
     6781 ttcccagata cgacgcagat atggatgaag agacaatccc gcttgaagca ggactcctcg
     6841 atgcaataag ttttgagaag ggatgctacg tgggacaaga agtgattgca cgtattaagt
     6901 ggagaggtca tgtaaattgg tgtcttgttg gttttgaaat tgaaggcgaa accctgccct
     6961 caaagggcga taaaatcttt cttggtgaac gggaaatcgg tcatataaca agtagtgtat
     7021 tttctccagt acttaatagg gtaatagccc ttggatacat aagaagggag tttaaagatc
     7081 ctgagactaa ggttatagta aggataaaca gcgaaagcaa atcagcaacg gttgtaagaa
     7141 cgccatttct tcaaagtcgt ggttcgtgaa ttgtggctcg tgtatcgaat cacgattcag
     7201 tggtgggcgg tactggattt gaaccagtga cttccaccgt gtgaggatgg cactctgccg
     7261 ctgagttaac cgccccaact tttattttag tattgttttt aaaaagaaat caataaaagg
     7321 ctttcgaaca acgacgaaat cttagaaatc gtcttcaaca ataagtaatt tttcagcctc
     7381 ttctattatc tcgccgagcc gttctctgaa aagcttgatt ccaccaagtg gtattactat
     7441 gcaggatctt cctccactta gctcagttat cctcaagtat tttcctttat ggttttcttt
     7501 cacgtcgaaa aaaaacctct tggactcgat gtcaacatgc ttgctaaggt gttccttttc
     7561 taacttttcc atttttctac ctccaccact tcaccttctc cacatgaatt ttaaaatcgg
     7621 gttagaataa taactccata ctagaaaaaa atcaagacat aatatttatg cgtcatctaa
     7681 gacataaacc attgatattc atttgctgaa gaacataaat tcgctactat catcaatgca
     7741 cctttcctat gagagattac acaaataaat acttatttaa caaataagca atattcattt
     7801 agtagcttaa tcattgtgtg ttgtatgata ggattaagct aaagaaataa ggagcgtgag
     7861 ccatggaaaa aaagttaaaa tttggaatcg caggtcctgt gcctggagaa tcagtagcta
     7921 atttaattaa ctactcaatg ctctatgaaa gaggcggctt cgatacggtc tggtttcctg
     7981 atcacgtcgt atttatggct aaaacactaa caccagaagt atggtctgta attacagccg
     8041 catgtctaaa aacaaaaaag atcttgatgg gaacgattgg tgatgcacac agaattcacc
     8101 ctgcaatatt tgcccatcgt cttgcaaccg ttgatcatat ctcaagcgga agaatttttg
     8161 tctgtgtcgg atacggagaa aaaatgaatc ttgatcctta tggaataaga tgggataaac
     8221 cattaaagcg cgttgtagaa
//
LOCUS       DATDRL010000192         2649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1081085, whole genome shotgun sequence.
ACCESSION   DATDRL010000192 DATDRL010000000
VERSION     DATDRL010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081085"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VI935_06655"
     CDS             complement(<1..776)
                     /locus_tag="VI935_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLE25315.1"
                     /translation="MARPLRFECPGAIYHVTIRGNARQPIYEDGENRKAFLELLGFIV
                     DRFNWLCHAYCFMDNHYHFLIETLDGNLSLGMRQLKGIYTQRFNRRHGRVGHLFQGRF
                     KAILVDGENYLLELCRYVVLNPVRAGMVRSPGRYKWSSYHATVGLEKISSFLTTEWIL
                     SQFGRSRKVAREKYKTFVEEGIGKPGPWGKLRGQILLGDDTFVESLEPYIKKVREVEE
                     VPRGQRFVDRPGLEELLRDVEYQPNAVRDNLIREAHWKHW"
     gene            complement(944..1903)
                     /locus_tag="VI935_06660"
     CDS             complement(944..1903)
                     /locus_tag="VI935_06660"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HLE25316.1"
                     /translation="MTKRVLVTGGCGFIGSTLIRVLLRRGYTVTVFDNLSSGANENLP
                     EYKGISLEVGDIEVYTSVKNAIQGHDFVIHLAARAYIPGSFKNPVEYFRTNVFGTLNM
                     LKACLNNRVKRLILASSAEAYGSAMVTPIDESHPLNPMSPYASSKLAAEIVAQSFLRH
                     FGLPVVIVRIFNTYGPREGLPYVIPEIIRQCVKETVIRIGNLEAERDFTYVEDMALGL
                     ALTLETKQIEGEIINLGYGQSWSISAVLDKIRRILNKDLPVIVDPSRLRPLEVAKLLA
                     DNSKAEKLMGWRPVVNLDDGLKRTVAWYIEHGNQWTYEKLLKK"
BASE COUNT          749 a          650 c          499 g          751 t
ORIGIN      
        1 taccagtgct tccaatgagc ctcacgaatc aaattatctc ttactgcatt aggttgatac
       61 tccacatccc ttaatagctc ctctaaccca ggcctatcta caaatctctg tcctctcggc
      121 acctcttcca cttcccgtac cttcttaata taaggctcaa gactttcgac aaaggtgtcg
      181 tcccccaaaa gaatctgtcc tcttaactta ccccacggac ctggcttacc aattccctcc
      241 tccacaaacg ttttatactt ctcccgcgct accttccgac ttctcccaaa ctgggacagt
      301 atccattccg tggtcaaaaa tgacgaaatt ttttccagcc ctactgttgc atgataacta
      361 ctccatttgt accgaccagg tgacctcacc atacctgcac gcacaggatt caagaccaca
      421 tagcggcaaa gttcaagaag ataattctct ccgtctacca aaattgcttt aaacctcccc
      481 tgaaacaaat gtcctacacg cccatgtcgt ctgttgaacc tctgagtata tattcccttt
      541 aactggcgca tacccagtga aaggttcccg tccagagttt caattaagaa atggtagtga
      601 ttgtccatga agcagtaggc atgacagagc cagttgaagc gatccactat gaaacccaat
      661 aattctagaa aggccttgcg gttttcaccg tcctcataga taggctggcg tgcattgcca
      721 cggattgtga cgtggtagat agcacctgga cattcgaatc ggagaggtcg agccacaggt
      781 agaaattatg gatcaaaaaa tcttatattt caagacctga ccctattcat acttgacagg
      841 gactggctaa tgggtgaccc cgtatgttcc agaaaaggtt tgcaagtatt ttaatccaac
      901 acacaataaa tgctcgacta gccctttctg aaaaggtccg accctatttc ttcaatagtt
      961 tttcataagt ccactggttg ccgtgttcga tataccaagc tactgttctc tttaatccat
     1021 cgtccaaatt aaccacgggc cgccaaccca ttagcttttc tgctttgctg ttatcggcta
     1081 gaagtttagc tacctctaaa gggcgcaacc gactcggatc aacgattacc ggtaaatctt
     1141 tattgagaat gcgtctaatc ttgtctaaga ctgcactaat gctccacgat tgtccatatc
     1201 ccaaattaat tatctctcct tcgatctgtt tcgtttcgag agtaagagcc aatcctaatg
     1261 ccatgtcttc cacgtatgta aaatctcgct ccgcctccaa atttccaatc cgaatcaccg
     1321 tttccttcac acattgtctt ataatctcag ggatgacata gggcagtccc tcacgcggtc
     1381 cataagtgtt aaaaatacgc acaatgacta caggcaaacc aaaatgacgc aagaaacttt
     1441 gagcgactat ttctgcggct agcttggagg aagcatatgg actcatggga ttcaagggat
     1501 ggctttcgtc tatcggcgta accatggctg acccatacgc ttcagcgcta ctcgctagta
     1561 tcaatctttt gactcgatta ttaaggcagg cctttaacat gttaagtgtc ccaaatacat
     1621 tggttctgaa gtattccacg ggatttttaa agctacctgg aatatatgca cgagcagcga
     1681 gatggattac aaaatcatgc ccttgaatgg cgtttttaac tgacgtataa acctctatgt
     1741 ctccgacttc caagcttatt cctttatatt ccggcaaatt ttcattggct ccagagctaa
     1801 ggttgtcaaa aacggtgacg gtataaccac gcctaagtaa tacccttata agggtactac
     1861 ctataaaacc gcatccgccg gtaacaagca ctcttttcgt catcctaagc tattaacgat
     1921 ggatattacc aaaagtagta attgtacaat atggctgaat agcataacgc aaagaaggta
     1981 attatacgca cttctcttcc agacttttgt ttccagggga cgaagcagtt ttaacagaca
     2041 atttgttgga gaagactcca tttgtttaac atccgatgtt ttagtagggt agtcattcag
     2101 tgtaatacac gaaagttttt ttattagttc gtttgcctcc ctcattcttg acatctgaaa
     2161 aaaccgattt ttgatcaggg ctaaggttaa aggagcagtt agtacaatca aaaaaaatga
     2221 tgcaaatatt gagcccaatc ctttcacatg tgtattcaaa ataaaagcga ccactgcacc
     2281 tgttatgacg taactagctg ccggtatctg ccatatcatg atatcaaagt tccgaatatc
     2341 gaatcgacat gacgtgtata gcgcaagcag gacatcccgg ttagagttag tcgaaggttg
     2401 ggaaccgagg ggggatgcct cgttagtcat tgctcatctc ctccatcatt ctgagtacat
     2461 ctctcaacac tcatcctatg gaactcttgt acctatcgct ctggtgaggc ctaacgtatt
     2521 atgaaagggc gccccttcag tggtagtctg aagttgcaat ttagacaaaa aaccacttat
     2581 taaggagggc cctatgaaca atgataagta tattggacta gatgttcatc agtcaagcgt
     2641 agtcacagc
//
LOCUS       DATDRL010000193        13479 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3919332, whole genome shotgun sequence.
ACCESSION   DATDRL010000193 DATDRL010000000
VERSION     DATDRL010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13479
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3919332"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VI935_06665"
     CDS             complement(<1..1008)
                     /locus_tag="VI935_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HLE25317.1"
                     /translation="MEISVCYLFKYIYYLKEVNVGGQLFKNLILWLVIFVTVVALWQF
                     VKSLSPQITYDKIGYSDFLARLEQGEIKEVVFKGDEIEAYEAEKDKSKKGFKTVGPAN
                     EDIISDLKTKGIVFSFKEQKEGSLMQILFNWAPLILLVVIMVLFMRQLQAGGTKAMSF
                     GRSRARMVSENQNKTTFKDVAGIDEAKEEVQEIVEFLKNPKKFTRLGGRIPKGVLLVG
                     PPGTGKTLLAKAIAGEAGVPFFIISGSDFVEMFVGVGASRVRDLFIQAKRHVPCIVFV
                     DELDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFEPNEGIIVLSATNRPDVLDPALL
                     RP"
     gene            complement(1035..1979)
                     /gene="cysK"
                     /locus_tag="VI935_06670"
     CDS             complement(1035..1979)
                     /gene="cysK"
                     /locus_tag="VI935_06670"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HLE25318.1"
                     /translation="MSENVVNDISSLVGDTPVIKLNRVVPKGVATIWAKLENLNPAGS
                     VKDRICLSMIDAAEKEGKIRPGSSVIVEPTSGNTGIGLALICAVKGYRLILTMPEDMS
                     LERRLLLAAYGAELVLTPANEKMEGAVKKAEKIVWENKNAFMPQQFKNPHNPEIHRLT
                     TGPEILKQVPGEIHALIAGVGTGGTITGVGEILRKKFPNIHIVAVEPAESAVLSGGMA
                     NVHDIQGIGAGFVPEVLNTKIYDEVIKITGEEAKRFTKLISRKEGLLVGISAGAAALA
                     SVRIAERLGSGRQVLTIFCDTGERYLSTGIFCENEYDN"
     gene            complement(2697..2969)
                     /locus_tag="VI935_06675"
     CDS             complement(2697..2969)
                     /locus_tag="VI935_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25319.1"
                     /translation="MKRTYKTNSKINIKQGSVIIKAMEQKSQDIDELDSYIRGLNDQE
                     LKGVLLKLKNELRKPGASWEIVRKTLYSLWNKNKDTFLDIIPLILK"
     gene            3158..5659
                     /locus_tag="VI935_06680"
     CDS             3158..5659
                     /locus_tag="VI935_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HLE25320.1"
                     /translation="MYWYFTHDLPDLTKITGYKPYLTTEVYSSDGRIIGEFGTERRKL
                     IPYEEIPPHVKNAFIAVEDKRFFEHKGVDLRRIIGALLKNIEEGEIVQGGSTITQQVV
                     KNLVLSPERSITRKIKEAILAYKMEKNLSKEEILYIYFNHIYFGDGTYGIESASRNYF
                     GKFATEINLAQATFLAALPKAPGYYNPRLHFHRAKKRQELILKIMEDEGFANKEQIKQ
                     AQEYKIAIAPRRNTNLEVAPYFAELVRQHIENKYGSKALIEGGYTVFTTLDIDLSLAG
                     ERVLKEGIMKLDSRQGNPIVIKHLNNQKEIDTYRDSQNIGSLEKDKSYQGVVLSATKD
                     NSTNIFTAVIGVGKYLGNLKFIVSSLLPNLQENPQNSLSNKYTSPYIDLLPFELRVGD
                     VVKVKALKKEKGINIMSLGFIPESQGALLAMDVSSGEIKAMIGGFDFTDSQFNRATQS
                     LRQPGSSFKPIVYAAAIDKGYTETTFVYDMPVTIKDWVPQNYDGSYLGEIPMREALAK
                     SRNLASVRILMDINPSYAVNYAKKLGFTSHLSPYLALALGGSDVKLIEMVKAFNVFAS
                     GGRLVEPKFILRIYDRDGKLVEEDTTWKYITTEEAEKAERGQQRLDILNQIAKSLGRS
                     VFTKETKTKEDDLAQNNLDDEDSKSEFLTPKEFLRLIQKGDSKGLTPSKIGEQVISPE
                     TAYIMTDMLQAVIREGTGRSALELTSIAPVAGKTGTTNDFTDAWFIGFSPKIIAGVWV
                     GNDNHKSIGEGEVGGKAALPIWIGFMKAALSKYPSGNFKVPDRIQFVSTPYGFIPYKF
                     DSSPTTIDFGGTANNYEDTASTSSSGSEIDFLIRR"
     gene            complement(5656..6186)
                     /locus_tag="VI935_06685"
     CDS             complement(5656..6186)
                     /locus_tag="VI935_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HLE25321.1"
                     /translation="MAKIKTSILFLFHNQPVLCLAFCCLMFYLIQNYSFKDPHKVKNR
                     PSSSNFYIEFAKADGFPVLYDIGNSQELERVVPSSVYTKIKSGDKVIIHDNGTTSLSR
                     ISGKKSLALGIPTGLNSASIDDLTALPGVGIKLAERIVEYKKLNGSFKSVDELDNVKG
                     FGKKKIEAIKPFINLD"
     gene            complement(6246..7037)
                     /locus_tag="VI935_06690"
     CDS             complement(6246..7037)
                     /locus_tag="VI935_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLE25322.1"
                     /translation="MDLGIENKVAIVAASSKGLGRAIAFRLGGEGAKLTIFARGKSDL
                     ENTAKQIELKTGAEVLSIVADVSKIDDISKIVSQTVNRYGTVHILVNNAGAPPLGDFL
                     SFSREDWLRALELNLLSTINLTREVISHMQRQKWGRIVNITSSAVKQPIDGLILSNTA
                     RAGVIGFAKSISNEFAKDNILINNVCPGRILTDRIIQLAEERAKRNNIEVEKVIRSWE
                     QEIPARRLGKPDELANLVAFLVSEQASYITGTTIQVDGGMVRGIF"
     gene            7539..8117
                     /locus_tag="VI935_06695"
     CDS             7539..8117
                     /locus_tag="VI935_06695"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HLE25323.1"
                     /translation="MIFNSNIALKEWAVTIKALDEGIQTILFRKGGIHEEGRKFRIEH
                     DRFLLYPTYEHQREELLKDKFRKELYTVLHDSDDTNTQKIRNWATISHIAEITQMAQI
                     NALSPYYIWNNDYLYERLKWRPRKPLYMLFLKVYRLLEPKTIEITPEYSSCKSWVKLN
                     EEFLLEDAKPVLSDLEFNQSLKTIKTILLESL"
     gene            complement(8236..8562)
                     /locus_tag="VI935_06700"
     CDS             complement(8236..8562)
                     /locus_tag="VI935_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HLE25324.1"
                     /translation="MPIYEYECEKCNRITEVLQGFNDPPLKKCKYCKGRVQRIISLSS
                     FQLNGTGWYATDYAKGPGIPPDIAKDNPALSDSNNGSTKKDEESKPKKPRKSGLPNME
                     KSDVKI"
     gene            8987..9553
                     /locus_tag="VI935_06705"
     CDS             8987..9553
                     /locus_tag="VI935_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25325.1"
                     /translation="MVLHLNLINILLSSLILIFISCKSDVRDTTSKSSTNVSENVRTY
                     TDTFAYCKAVVNIDSPDSRYVGSKVPEVIARGLQKAFDVPPGAPLDVFTRGTYWRWMD
                     GKVYACNVGANLPCDQKADTSYKPNQGMVKWCESNLDSEFIPAYASGRATIYEWRCKG
                     KEPMIVREITKPDAAGYISNIWHEITPD"
     gene            9670..10398
                     /locus_tag="VI935_06710"
     CDS             9670..10398
                     /locus_tag="VI935_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLE25326.1"
                     /translation="MRLKDKTALITGAGGGIGKAIAKAYVEEGASVIICGRNEESLKE
                     ACKVISSYGEIEYVKADIVKKDDVKRLANRVEEKWNKLDILVNNASVLGVRTSILDYP
                     EDIWEEVINVNLNGQFFVIKAVLPFLLKSNNGSIINVSSSVGRRGKALWGAYAASKFG
                     LEGLTQVLADELKASSVRVNSVNPGGTRTEMRARAYPDEDPMTLPAPHDITPIFLYLA
                     SDKSIGVTGKPFDARDWNGRGSNS"
     gene            complement(10513..10695)
                     /locus_tag="VI935_06715"
     CDS             complement(10513..10695)
                     /locus_tag="VI935_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25327.1"
                     /translation="MQNLETTNLAGTIKDDVRKVVGVGSSAYRKVTSRNVVRSKTDPV
                     LPAAIPSYIVMLLDTP"
     gene            complement(10922..12196)
                     /locus_tag="VI935_06720"
     CDS             complement(10922..12196)
                     /locus_tag="VI935_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HLE25328.1"
                     /translation="MQSIFGLVDCNNFYVSCERIFNPKLKGKPVVVLSNNDGCVVARS
                     EEAKALGVPMGAPAFKYECLFKTHKVETYSSNYTLYADMSRRVMATLAQFTPEIEFYS
                     IDEAFLDLSGFSHLNLTEYARQIKARVMKWTGIPISIGIGPTKTIAKLANKLAKKNPM
                     CGGVMDITNHPRLDDFLQSVDVEDIWGIGRQYSRLLKRSGINTALDLKNASDVWVKKH
                     MTIVGLRTVWELRGIPCIELEDLGEPNKQIIRSRGFGRPVDNLAELREAVTFHTTRAA
                     ERLREQKLIASHISTFIETNRFKAEEPQYFKSAGYYLPEPTAYTPLLIKYALKNLERI
                     YKEGYRYTKAGVMLMGLTPEDQVQLNLLVPARPIEKDREIMKAVDKINVGWGSNTVKY
                     ASSGIEQQWRMRRARLSQRYTTNWDEMLVVKN"
     gene            complement(12200..12628)
                     /gene="umuD"
                     /locus_tag="VI935_06725"
     CDS             complement(12200..12628)
                     /gene="umuD"
                     /locus_tag="VI935_06725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HLE25329.1"
                     /translation="MNVIEVYRLKTEARVKLPLILARTQAGFPSPADDYIEERIDLNK
                     KLILHPEATYLVRVMGDSMIKDRIHEGDYLIVDRALEAGHNDIILAVLNREFTVKRLE
                     KTTHRWYLVAGNDKYPPIEINPEMDFEIWGKVTYIFHPAV"
     gene            complement(12664..13350)
                     /locus_tag="VI935_06730"
     CDS             complement(12664..13350)
                     /locus_tag="VI935_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017437509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HLE25330.1"
                     /translation="MCGRMTLTTDKDDIQSRWGFIDPSGVLDLIKPRFNISPSQNSPT
                     VIVNQDNRELKMMRWGLIPFWAKEASIGYKMINAKAETVHEKPSFRKSFKDKRCLVLA
                     DGFYEWTKTDKKNKIPFRFVLKTKEPFAFAGLWDAWKTPEGEMLLTFTIITTNANELM
                     EPIHDRMPVILHEKDEALWLDPEFKDANKLTALLKPFPSDKMEAYRVSTIVNSPKNDT
                     PKCIEPVGSM"
BASE COUNT         4041 a         2771 c         2501 g         4166 t
ORIGIN      
        1 aggacgaagg agagccgggt ctaatacatc agggcggtta gttgcagata aaactataat
       61 tccctcattt ggctcaaacc catccatctc gacgagaagt tgattaagag tctgttccct
      121 ttcatcgtgt ccacccccaa gtccagctcc acgatggcga cctacagcat caagctcatc
      181 aacaaaaaca atgcaaggca catgacgttt tgcctgtata aaaagatcgc gcaccctaga
      241 tgcgccaact cctacaaaca tctctacaaa atcgctacca ctaattataa agaatggcac
      301 acccgcttca cctgcaatgg cttttgcaag gagtgttttt cccgtacctg ggggtccaac
      361 gagaagcaca ccctttggta ttctaccccc aaggcgtgtg aatttctttg ggttctttaa
      421 gaactctacg atttcctgaa cctcttcctt tgcttcatca attcctgcga cgtctttaaa
      481 tgtggtttta ttctgatttt ctgaaaccat tctggcgcgg cttcttccaa acgacatagc
      541 tttagtacca ccagcttgga gctgtctcat aaataaaacc ataatgacta ccaaaaggat
      601 aagcggtgcc cagttaaata ggatttgcat tagcgaaccc tctttttgct ctttgaagct
      661 gaatactata ccttttgtct tgaggtctga gataatatcc tcgtttgctg gtcctacggt
      721 tttaaaccct ttttttgatt tatctttctc agcttcgtat gcttctattt catccccttt
      781 aaatacaacc tccttgattt ctccctgctc taaccttgca agaaaatcac tgtatccgat
      841 tttgtcatag gtgatttgtg gactcagact cttaacgaat tgccataatg caacaacagt
      901 tacaaaaata accagccaaa gaattagatt tttaaaaagc tgtccgccca catttacctc
      961 ttttaaatag tatatatact taaaaagata acacactgaa atctccattt caacatttta
     1021 tcggttccag attcttagtt gtcgtattcg ttttcacaaa aaatcccagt acttaaatat
     1081 ctctctccag tatcgcagaa tatcgttaga acttgcctcc cgctacctag tctttcagca
     1141 atcctcactg aagcaagtgc agcagcccct gccgatatgc ctacaagcag tccctcttta
     1201 cgggaaatga gctttgtaaa cctttttgct tcttctcctg ttatttttat tacctcatca
     1261 tatatctttg tattaagaac ctcgggaaca aatccagcac cgattccttg aatatcatgg
     1321 acgttcgcca taccacctga aagtacagcc gattcagcag gttcaactgc tactatgtga
     1381 atgtttggga atttctttcg aagtatttct cctaccccag ttatcgttcc tccagttcca
     1441 acaccagcta taagagcatg aatttctccc gggacctgtt ttaagatttc aggtcctgtc
     1501 gttaatctat ggatctcagg attatgtggg tttttaaatt gttggggcat aaaagcattt
     1561 ttattttccc atacgatttt ttcggctttc ttgacagcac cttccatttt ctcattagca
     1621 ggtgttagaa caagctcagc tccgtatgca gcaagaagta gtcttctttc taaactcata
     1681 tcttcaggca tcgtgagaat taacctatat ccctttacag cgcaaataag tgcaagcccg
     1741 attcctgtgt ttccgctggt aggttcaaca attacgctac tgccaggcct tatctttcct
     1801 tctttctcag ccgcatcaat catactgaga caaattctat ccttaacgct gccagcggga
     1861 ttgaggtttt cgagctttgc ccaaatcgtg gctactcctt ttggaacaac cctatttagc
     1921 ttaattacag gggtgtcacc aactagactt gatatatcat ttacgacgtt ttcactcatt
     1981 atgattcctt gtattttttg acgttctgat tgaacttgac tatttcctgt agcttgttac
     2041 aggtttccta ctgtcattgc gaccccagat gaagtctttg ggggaaagca atctggcacg
     2101 aagtgtcatt ccgaaccatg tcctgagccg aagccctgaa cgtagtaaaa ggcctggcga
     2161 aggagcagtg aggaatctct atcagattgc tccctccctt gcgctttgct cagggctttg
     2221 actcatttgc tgctcgcact tacagaagaa gttgtttgtt ttaacgctaa taccatacag
     2281 cttgctgttg ggatcttaat tagaaataat aatttaatca tatggaatac ctatttcaag
     2341 caaccgtaat taaatctaga tggaataaac tagtaaagtg ttatactcaa aggtctattt
     2401 agattggtat gtgaaggata gaaaagatgc agaagaatta atagcagaga ttaaaggaaa
     2461 ataattagtg ttttgcagaa ttgtcaagtt ttagtctatc tcagcattcc tcgctcttat
     2521 tatatatttg tatcctcaat tgtaagggta cgccgcaacg tgcccttcat ttcaccccat
     2581 ttgtcattcc cgcacgcttt aatcgggaat ccatatgttt attacctttt gcaatacaac
     2641 atagcggcga cctttaggtc gccgtaaaga aaaatatgga aggctaaagc cttccgctac
     2701 ttcagaatca aagggatgat atctagaaaa gtgtctttat tcttattcca caaagagtaa
     2761 agcgttttcc ttactatttc ccaagaggcg ccaggttttc ttaactcgtt tttaagtttg
     2821 agcaatactc ctttgagttc ctgatcattt aatccacgga tgtagctatc taattcgtca
     2881 atatcttgac tcttttgctc catggctttt attataacag atccttgttt tatattaatt
     2941 ttactattag ttttataagt ccgtttcatc aatctaatta ataaatactc taagcaggaa
     3001 gagacaaaac ttgatattgt gaagctatga ctgtaaatta aatatattta aaaataaaat
     3061 gaagcggcaa ataaacctgc gcaaaagaag caaaattaaa tcaactatta tttcttttac
     3121 tatactttta ataattactt ttgtgttcat ttacagtatg tactggtact tcactcatga
     3181 cctgccagac ctcacaaaaa tcacgggcta taaaccctat cttacgacag aagtctactc
     3241 atcggatggc cgaataatcg gagagtttgg aaccgaacgc aggaagctaa tcccatatga
     3301 agaaatccca ccacacgtaa aaaatgcatt cattgcagtt gaagataagc gcttttttga
     3361 gcataaaggg gtcgacctta gacgtattat aggcgcgtta ttaaagaaca ttgaagaagg
     3421 tgagatagta caagggggga gtacaataac ccaacaggtt gtaaagaatc ttgtcctctc
     3481 accagaaagg agtatcacaa gaaagataaa agaggcaatc cttgcataca aaatggagaa
     3541 gaatctttca aaagaagaga ttctttatat ctacttcaat catatttatt ttggagatgg
     3601 cacctatgga attgaatccg caagcagaaa ctactttggc aagttcgcta cagaaataaa
     3661 tcttgcacag gctacatttc ttgctgcatt accaaaagcg cctggatact acaatccgcg
     3721 cctgcatttc catagggcaa agaaaagaca agaactcata ctaaaaataa tggaggacga
     3781 aggattcgca aataaggagc aaataaagca agcacaagaa tacaagattg caattgcacc
     3841 aaggagaaat actaatcttg aagtggctcc atattttgca gaactcgtta gacaacatat
     3901 agagaacaaa tatggttcaa aagctcttat tgaaggggga tacacagttt ttaccacact
     3961 ggacattgac ctaagcctag ccggagagag ggttttaaaa gagggaatta tgaaactcga
     4021 ttcaaggcaa gggaatccta ttgtcattaa gcatttaaat aatcaaaagg aaatagatac
     4081 atacagagat tcacaaaata tcggcagttt agaaaaggac aaatcttatc aaggtgtagt
     4141 tctcagtgcc acaaaagata attcaacaaa tatttttacc gcagtaatcg gggttggtaa
     4201 atacttaggg aatctcaagt ttatagtaag ttcactccta ccaaatctac aagagaaccc
     4261 tcagaattca ctttctaata aatatacgtc accttatata gatttactcc ccttcgaatt
     4321 acgagttggc gatgtagtta aagtaaaagc tctcaaaaaa gaaaaaggga tcaatattat
     4381 gtcactaggt ttcatacctg aatctcaggg ggcactctta gctatggatg tgagctcagg
     4441 tgaaattaag gcaatgatag gtgggttcga tttcacagat tcacagttta acagggcaac
     4501 acagtcactt agacaacccg gctcttcatt taaaccaatt gtgtatgcag cagcaataga
     4561 taaaggctat acagaaacga cttttgttta tgatatgcca gttacaatta aggattgggt
     4621 tcctcagaac tacgatggca gctatctagg tgagattcct atgagagaag ctttagcaaa
     4681 atcaagaaat ctagcaagtg tgaggattct aatggatata aaccctagtt acgcagtaaa
     4741 ttacgcaaag aagcttggct tcacctctca tctaagtcca tatctagcac ttgcccttgg
     4801 tgggtcggat gtaaagctta tagagatggt aaaggcattt aacgtctttg cttctggtgg
     4861 tagactcgtc gaacctaaat tcatacttcg tatatacgat cgagacggca agctagttga
     4921 agaagacaca acctggaaat acattactac agaagaggcc gaaaaggccg agaggggaca
     4981 acaaaggctt gatatactta atcaaattgc aaagagtctc ggtagaagcg tttttacaaa
     5041 ggaaaccaaa actaaagaag atgaccttgc tcaaaataac ctagatgacg aggattcaaa
     5101 aagcgaattt cttactccaa aggagttttt gaggcttata caaaagggtg attccaaagg
     5161 attaactcct tccaagatcg gagaacaagt tataagtcct gagacagctt atataatgac
     5221 tgatatgctt caagcagtta taagggaagg gacaggcaga agtgcgcttg aacttacctc
     5281 aattgctcca gttgcgggaa aaaccggaac tacaaacgat tttacagatg cttggtttat
     5341 cgggtttagt ccaaagataa ttgctggagt ctgggtaggc aacgataatc acaagtcaat
     5401 cggagaaggg gaggttggtg gaaaagccgc tcttcctata tggataggtt ttatgaaagc
     5461 agcacttagt aaatacccaa gcggtaactt taaggttccg gatagaatac aatttgtaag
     5521 cacgccctat gggtttatac cttataaatt tgattcatcc cctacaacaa ttgattttgg
     5581 aggaacggcg aacaactatg aagatactgc atcaacatcc tcatctggaa gcgaaattga
     5641 ttttctaata cgccgctaat ctaaattaat aaagggtttt attgcttcaa tctttttctt
     5701 accaaatccc tttacattat ctagctcatc aacgctttta aagctaccgt ttagcttttt
     5761 atattcaacg attctttcag ccagtttaat tccgacgccc gggagcgccg ttaagtcatc
     5821 tatactagcc gaatttagcc ctgtaggaat accaagagct aggctctttt taccacttat
     5881 tctagaaagt gaagtagtgc cattatcatg aataataacc ttatcaccgg attttatttt
     5941 tgtataaaca gatgaaggaa ctacacgttc tagctcttga gaattaccta tatcatacaa
     6001 aacaggaaac ccatcagcct tagcaaactc gatataaaaa ttagaggacg acggcctatt
     6061 tttaacttta tgcggatcct taaaactata attttgaatc agataaaaca ttaagcagca
     6121 aaatgctaag cataagactg gttggttatg aaaaagaaat aagatactag tttttatttt
     6181 tgccattgat ctaagtacct ttctctttat atcattcaat gacaagtttt ataaattaag
     6241 acttgctaaa agattcccct taccattcca ccgtctactt ggatagtcgt ccctgtaata
     6301 tagcttgctt gctcagaaac aagaaatgca acaaggtttg caagctcatc tggttttcca
     6361 agtcttcttg caggaatttc ttgctcccat gatcttatta ctttttcaac ctcaatattg
     6421 tttcttttgg ctctctcctc agcaagttga ataattctat ccgtaagaat tcgacctgga
     6481 cagacgttgt ttataagtat attatctttc gcaaactcat ttgaaatgct ttttgcaaac
     6541 ccaattactc cagcccttgc tgtgttagag agaattaaac catctatagg ttgttttaca
     6601 gcggaagagg tgatattaac gatccttccc catttttgcc tttgcatatg agaaattact
     6661 tcgcgtgtca aatttatagt gctcagtaaa ttaagttcta aagctctgag ccagtcctca
     6721 cgagagaaac tgagaaaatc cccaagagga ggggcgccgg cattatttac aagaatatgg
     6781 acagttccat atctattgac tgtttgagaa acaattttac tgatgtcatc tatcttagag
     6841 acatccgcta ctattgataa aacctccgct ccggttttta actcaatttg ctttgctgta
     6901 ttctccaggt cagactttcc tcttgcgaag atagttagtt ttgctccttc tcccccaagt
     6961 ctaaaggcga ttgccctacc taatcccttg cttgaagctg ctactattgc aaccttgttt
     7021 tctataccta gatccatatt tttagaacct ataattttta attgaaaaca atataaactc
     7081 aataaataaa tagaacaagt ttacttagga acactgcagc aggggtatca gcatttagac
     7141 ggacaaatct ttcggtcatt gcgaggaacc cttcgacgaa gtttaccctg agcgaagccg
     7201 aagggctcag tataaactcc gtgaagtaat tctatgttgc ctgtcattgc gagtgttagc
     7261 aaagcaatct cgtctttttt ggattgtttc aatgcttcct tcttttattt acatttctcg
     7321 ttcttttcat ttccctttga accacactgc ggttcaaata atactgcgct cgaaataatt
     7381 cacctcacaa tgacattccc gtattctcat aaagatgata atggaaaccc tgtagcaaag
     7441 tatagggaat tgtcgagcta aattcatatg aaagcatttt ggtatgcaaa catttttggt
     7501 gctataatca tcttgtgaat atctaatgag gggaatgcat gatatttaac agcaacattg
     7561 cccttaagga atgggcggtt actattaagg ctctagatga aggaatacag acaatcctct
     7621 ttagaaaagg tggaatacac gaagagggta ggaaattcag aatagagcat gatagatttc
     7681 ttctttatcc aacatatgaa caccaaagag aggaattact taaagataaa tttaggaagg
     7741 agctatacac ggtgcttcat gactcggatg atactaatac acaaaaaatt agaaattggg
     7801 ctactatttc gcacatagca gaaataactc aaatggcaca aatcaacgca ctatcaccat
     7861 actatatatg gaataatgat tatttatatg aaagactcaa atggcgtcca aggaaaccac
     7921 tttatatgct atttttaaag gtatacagac tcttagagcc aaaaactatc gagataacac
     7981 ctgaatatag cagttgtaaa tcctgggtga aattaaatga agagttctta ctagaagatg
     8041 caaagccagt attgagtgat cttgaattta atcaaagttt aaaaacgata aaaaccattt
     8101 tactagaatc cttatagagt gtttatattg taggttagat gtaagggtta atatacgaag
     8161 aaaaaattaa cttatgagcc tataggttaa ataattttaa taattattac tggtagttag
     8221 agccaatcct taaatttata ttttaacgtc acttttctcc atatttggca gaccagattt
     8281 tctaggcttc ttaggtttcg attcctcatc ctttttagta ctgccgttgt tagaatcaga
     8341 aagggcaggg ttatctttag ctatgtctgg tggtattcca ggtccctttg cataatctgt
     8401 ggcataccat ccagttccat taagttgaaa tgaggataaa gaaattatgc gttgtaccct
     8461 tcccttgcaa tatttacatt tttttagggg agggtcatta aaaccctgaa gtacttctgt
     8521 tatccgattg catttctcac attcatattc ataaatcggc attgtttata ctcccttcga
     8581 attgtcttta tttatagtct atttagtata ataataaaaa tctaaacatt caaggcatcg
     8641 aatctaaagt gatcgtatct aaagaagcta atgagaaggc tagctttcat ccttatgatc
     8701 atttctttac ttgaaacgag gatcacgtca taaagaacta ggaacagttt ctgtaaatgc
     8761 cggagcagca tttgcgttat gagtatttca ttgttatagg gtcttgaaat ataactcaaa
     8821 ttgagattgc ctagttgaca taatatatct tatcagacat aaatttagaa actcatattt
     8881 aaagcctttg aatttctctt aagcgtaaga cgtttaagca aaagataatt gaagtaccaa
     8941 gaccgacgca ttacaatcta tacgaccaat ataataaaga gatgacatgg tgttacatct
     9001 caatctgata aatattcttc tatcatccct aattctgata tttatatctt gtaagagcga
     9061 tgtcagagac accacttcta agtcatcaac aaatgtttca gaaaatgtta gaacttacac
     9121 cgataccttc gcctactgta aagccgttgt taatattgac tctcctgatt caagatatgt
     9181 tggatcaaag gtacccgaag tgatagcacg tggattacaa aaggctttcg atgttccccc
     9241 tggagcaccg cttgatgtat tcacaagagg aacttattgg cgatggatgg atggaaaagt
     9301 atatgcttgt aacgttggag caaacctgcc atgtgatcag aaggcagata ctagttacaa
     9361 accgaatcag ggaatggtaa agtggtgtga atccaatctt gattctgaat ttatccccgc
     9421 atatgcctca ggtcgggcta caatctatga atggagatgc aaaggcaaag aacctatgat
     9481 tgtaagggag atcaccaagc ccgatgctgc tggctacata tccaatattt ggcatgaaat
     9541 aactcctgat tagaaattta tttctgctgt ttatctggaa atgaattaat tgtatttgta
     9601 aaccctacta ctaatacatc ttatacttat aataaaagat tcgcatatat ctgttatact
     9661 gaggtttcta tgaggctaaa agataaaaca gcgctgatta ccggcgccgg tggcggaatt
     9721 ggaaaggcta tcgcaaaggc ttatgtagaa gaaggcgctt ctgtaataat ctgtggacga
     9781 aatgaagaaa gcttaaagga ggcgtgtaaa gttatctctt cgtatggcga aattgagtat
     9841 gtaaaggctg atattgtaaa aaaagatgac gtcaaaaggc ttgctaatcg agtggaagaa
     9901 aaatggaata agcttgatat tctggtaaat aatgcaagtg tacttggggt tcgcacttct
     9961 atcttagatt accctgaaga catttgggag gaagtaatta atgtaaatct taatggtcag
    10021 ttttttgtga taaaggcggt tctccctttt ctgcttaaat caaataatgg ttcaattatc
    10081 aatgtttcat caagcgtagg aagacggggt aaagcgcttt ggggggctta tgcagcgtct
    10141 aaatttggac tggagggttt aacacaagtt cttgcagatg agctcaaggc atctagtgtg
    10201 agagtaaatt ctgtaaatcc gggcggaacg agaactgaga tgagagcaag agcctatcca
    10261 gatgaggacc ctatgacact acctgcacct catgacatca cccccatatt tttatattta
    10321 gcatctgata aatcaattgg cgttaccggg aaaccgtttg acgcaaggga ttggaacgga
    10381 agaggctcta attcttgata tatcgcaact taaagcagca tcataatatt cttaagcaat
    10441 tactttaaat atattaatat tttactttaa gttgtataaa cgagcgcaaa tagcactttt
    10501 gacccataaa tgctaaggcg tgtcaagcaa cataactatg taacttggaa ttgcagcagg
    10561 aagaacaggg tctgtttttg accttactac gttccttgag gttactttgc ggtaggctga
    10621 gctccctacc ccgactacct tcctcacgtc gtctttgatt gttcccgcaa ggttagtagt
    10681 ttcgaggttc tgcaaaacag tcactgcctc tcacttgact gtcttctcat cttccaccct
    10741 cgtagcttcg gtatcttctg agattcccac cacccgcccc ttggaacgtc taagcctttg
    10801 ccatctcctt cccttccacc tacccttttg taagacggtt tattgaattc ctaggttata
    10861 gcatttggag actacggctg ttcggcgacc tcgcttttta tgttcagctc catactgaaa
    10921 tttaattttt aaccaccaac atttcatccc agttcgtagt atagcgctgt gataatctgg
    10981 ctcgtctcat tctccattgt tgttctattc ccgaagaggc atatttaaca gtgttgcttc
    11041 cccaaccgac gttgattttg tctacggctt tcattatttc cctatctttc tcaataggac
    11101 gtgcaggtac taatagatta agctgtacct ggtcttcagg ggtaagcccc atgagcatta
    11161 caccggcttt tgtgtatcta taaccttctt tataaattct ttctaggttt ttgagggcat
    11221 atttgataag caaaggcgta tatgccgtag gctccggtag ataataccca gcactcttga
    11281 agtactgagg ctcttcggct ttaaatctgt ttgtttcaat aaaagtagat atatgagaag
    11341 cgatcaattt ctgctctctc aacctctcag cagcacgggt tgtgtggaaa gtaaccgctt
    11401 cccttaactc agccaaatta tctacaggcc tgccaaagcc gcgagacctt ataatttgtt
    11461 tattaggctc acctaagtct tctagttcaa tacacggaat accccttagt tcccacaccg
    11521 ttcttagtcc cacgatagtc atgtgctttt ttacccatac atcgctggca ttctttaaat
    11581 caagtgctgt gttaatccca ctccgtttca gtagtcttga atattgcctt cctattcccc
    11641 atatatcttc aacgtccaca ctttgaagaa aatcatcaag ccgcgggtga tttgtaatat
    11701 ccatcacccc gccacacatc ggatttttct ttgccagttt atttgccagc ttcgcaatcg
    11761 tctttgtagg tcctatccca atagagatag gtatcccagt ccacttcatc acccttgctt
    11821 tgatctgcct tgcatattct gtgaggttca aatgagagaa gcctgaaagg tctaagaagg
    11881 cttcgtctat ggaataaaac tctatctctg gcgtgaactg cgctagagtt gccattactc
    11941 gtctcgacat atcggcatag agggtgtagt tggaagaata ggtttctact ttatgcgttt
    12001 taaataaaca ctcatactta aacgccggcg cccccatagg tacaccaaga gccttcgcct
    12061 cctcgcttct agccacaaca cacccgtcat tattactgag tactactact ggcttccctt
    12121 tcaacttagg gttaaatatc ctttcgcatg agacgtaaaa gttgttacag tctactaacc
    12181 caaatatgct ttgcatgatt taaacagccg ggtggaatat ataagtaacc ttcccccaaa
    12241 tctcaaagtc catctcgggg tttatctcga tggggggata tttatcattc cctgctacca
    12301 gataccatct atgcgttgtt ttttctagcc tttttacagt aaattctcta tttaacaccg
    12361 caagtattat gtcgttatga ccggcttcca aggctctatc cacgatcaaa taatcgcctt
    12421 catggattct atctttaatc atagagtcgc ccatcacacg cacaagataa gtcgcttcag
    12481 ggtgtagtat gagcttttta tttaggtcaa ttctttcttc gatgtagtca tcggcaggac
    12541 ttgggaatcc cgcctgcgtt ctggctaaaa ttaaaggtaa ttttactctg gcttctgttt
    12601 ttaatctgta tacttcaatt acgttcataa cttaccccac cttacaatag gatagtgtat
    12661 atatcacatg ctgccaactg gttcaatgca tttcggagta tcattcttag gagagttcac
    12721 aatcgtagat actctatagg cctccatttt atcagatgga aagggtttta atagggcagt
    12781 cagcttatta gcatccttga attccgggtc tagccataag gcttcgtcct tctcgtgcag
    12841 gattacgggc attctgtcgt gtataggctc catcaactca ttagcgtttg ttgtaatgat
    12901 agtaaaggtg agaagcatct ctccttcggg cgttttccat gcgtcccata gacccgcaaa
    12961 ggcaaaaggt tcctttgtct tgagcacaaa tcgaaagggt attttgtttt tcttatctgt
    13021 ttttgtccac tcgtagaacc catcggcaag aacgagacac cttttgtctt taaatgattt
    13081 tctaaaactt ggcttctcat gcacggtttc tgcctttgcg tttatcatct tgtagccaat
    13141 tgaggcttcc ttcgcccaga aggggataag tccccagcgc atcattttta attctctatt
    13201 atcttgatta acaattacag taggcgaatt ctgagaaggt gatatgttaa accttggctt
    13261 gattaaatct aataccccac ttgggtctat aaaaccccaa cgactttgta tgtcatcttt
    13321 gtcagtcgta agtgtcatcc ttccacacat cggagaaacc attttaacat tttaccgatg
    13381 ccaattagaa atcggcctaa aacgctctcc ccaagtatct tgactggaaa ttaatatcct
    13441 tttaatctag gaagtactaa ttagataaag caacgtttt
//
LOCUS       DATDRL010000194         5257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1853839, whole genome shotgun sequence.
ACCESSION   DATDRL010000194 DATDRL010000000
VERSION     DATDRL010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853839"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(439..1467)
                     /locus_tag="VI935_06735"
     CDS             complement(439..1467)
                     /locus_tag="VI935_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLE25331.1"
                     /translation="MNTFYKLPGIKLNGVSLSLSLLTLIMLLGCVGETPQEPPKYGFY
                     KNEPVVFRSPYDDVWAATLQAVQELNWETSNADKTKGIIELKTSYVYTEVFGEYTRMY
                     VETNNVEMEKSKIRPYLTRISYYEKTAPPIPKFVRETLEVNVKSLGPTETEVKAYYKI
                     MPYYDYKIGYLGSVRSNGYLEKNLFNRIQEILAEEEIVIPPPPPPIAVPFEMEFELRD
                     IFFDFDKHYIRPDAEPVLRENAEILMKNPDMNILIEGYADIRGTDEYNLRLAQKRADA
                     TKSFLVSLGVAPSRITTASGGETIQFGAGTTEEAYQLNRRAHFIPVRPGSMPGAKIFF
                     KNANKPTL"
     gene            complement(1496..1834)
                     /locus_tag="VI935_06740"
     CDS             complement(1496..1834)
                     /locus_tag="VI935_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25332.1"
                     /translation="MMRLSLWIFVISILIFGFSRYTILEEYDLTAAQKHFTAIVRGLP
                     GITSAQWKTPISLWAQVSSKAVGSPPNSAKAQQLADILAERGRTALRQPFCVHIYQGS
                     ANELARSCVY"
     gene            complement(1970..2731)
                     /locus_tag="VI935_06745"
     CDS             complement(1970..2731)
                     /locus_tag="VI935_06745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLE25333.1"
                     /translation="MKLKNKVALITGGGIGIGKATAILFAKEGAKVVITGRTEKTLKH
                     VAEEARKEGLKIDYLVSDVAKEEDCKEAIDYTINKYGRIDILFNNAGVLYTGTTHETP
                     TEVWDKTFAVNARGTYLMSRYTIPHMLKQGKGCIINNSSVLGLKAVPGVAAYNATKGA
                     VTQLTRSMALEYSSKGIRVNAICPGTIVTPMVEGLFEQIGDRKAAEELFKSFHPIGRL
                     GRPEEIAHAVLFLCDDNVEFMTGTMLSVDGGWVAR"
     gene            3124..3312
                     /locus_tag="VI935_06750"
     CDS             3124..3312
                     /locus_tag="VI935_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HLE25334.1"
                     /translation="MKKIVEFYYDLGSPYSYLASTQIERICEKYNADLEWKPLLLGGV
                     YKETGNRAPLEVPNKRHT"
     gene            3309..3716
                     /locus_tag="VI935_06755"
     CDS             3309..3716
                     /locus_tag="VI935_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HLE25335.1"
                     /translation="MIKDLADWAGYYGVRFNFPDLFPLNGVKPMRGALVAKEKEKIRD
                     YTHRLFELYWIHGKDLSQDEVLEKAVRELGIDAGWFMQRIGEQDIKDNLREETVKAVK
                     RGAFGAPTIYVWDKMFWGNDRLIFVEEYLKGKF"
     gene            3863..5125
                     /gene="lysA"
                     /locus_tag="VI935_06760"
     CDS             3863..5125
                     /gene="lysA"
                     /locus_tag="VI935_06760"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HLE25336.1"
                     /translation="MWYDFQYKGGEYHVEDVSVKEIVEAVGTPCYIYSNSALEGDFDV
                     FREAFSEINPLICFSVKANSNLAVLKTYAKLGVGFDVVSGGELLRVLRAGGEPEKVVF
                     SGVGKTEDEIKAAIEAGILFFNVESVDELQIINDVAKRLGKKARIALRVNPDIDPKTH
                     PYISTGLMKSKFGIEIGKALDVYKNASKMEGIEVMGIDAHIGSQIFQLEPFVESLRKL
                     IELGDALKNEGITIQYIDIGGGLGISYKEGEMPPHPKEYADVIKREISGTPYKLVLEP
                     GRVLVGNAGILVTKVLYIKDGVSKKFVVVDAAMNDLIRPAFYDSYHEIITIDESLGDV
                     ETVDIVGPVCESGDFFAKDRGLPKVKRGDLLAILSAGAYGFVMSSNYNTRPRVPEVMV
                     RGEEFFVVRGRETYEDLFKGEIIPDFMK"
BASE COUNT         1571 a          927 c         1093 g         1666 t
ORIGIN      
        1 cctagagata ataaatttta gtcctatgcc gcttgctctc tatcctggaa cagctatatg
       61 tcagtttata tttcagagaa ccatcggaga agcgagctac gaagggaaat ttaaagaaca
      121 agacgaagaa aacttttaaa atagcatatt gtgctattcc ggcatcgaaa cattaagttt
      181 gaatatatga aaatgactaa gatattcatg ataaacacgc cgggaaactt agtagatgag
      241 gatttgaaaa agcgtgttct ttatctccaa tttaaacgaa taaccttata tataagaaaa
      301 tagcttctat accaattgac tttaagcaat ctggatttta cagcggatat ttatgtgctg
      361 agtagtcaga gcttctaagc actcagatta gaatgacgag ttctcaactg gatttggtat
      421 tattggtatt acaggtttct aaagggtagg cttgtttgca tttttaaaga aaatcttagc
      481 acctggcata gaccctggtc taaccggtat gaaatgagcc cttctattaa gttgataagc
      541 ctcttctgtt gtcccggctc cgaattgtat tgtctctcca ccgcttgcgg tagttattcg
      601 tgatggagca actcctagac ttactaagaa tgactttgta gcatcagccc ttttctgcgc
      661 aagcctaagg ttatattcat ctgtacccct tatatcagca tacccttcaa ttaaaatgtt
      721 catgtcagga tttttcatta gtatctctgc attctctcga agtacaggct ctgcatcagg
      781 tcttatgtaa tgcttatcga aatcaaagaa tatatcccgt agctcaaact ccatttcaaa
      841 tggcactgcg ataggtggag gtggcggggg tataacaatt tcttcttcgg ctagtatctc
      901 ttgaatccta ttaaagagat ttttctccaa gtagccgttg gatcttacag atcccagata
      961 gccgattttg tagtcatagt atggcattat tttataatat gctttcactt cggtttcagt
     1021 tggtcctaaa cttttaacat tcacttcaag ggtttctctt acaaattttg gaataggtgg
     1081 tgctgttttt tcatagtaag atatgcgtgt taggtagggc ctgattttgg atttctccat
     1141 ttctacgttg ttggtctcta catacattct tgtgtactcc ccaaacactt ctgtatatac
     1201 atatgaggtt tttagttcaa tgattccttt tgttttatct gcattgcttg tctcccaatt
     1261 tagttcttga actgcttgca gggtagctgc ccaaacatca tcgtagggac ttctaaagac
     1321 aactggctca ttcttgtaaa agccatattt tggtggttcc tgaggggttt cgccgacaca
     1381 gcctaataac attattaatg ttaaaagaga caaggataga gagacaccgt ttaatttaat
     1441 tcccggcaat ttataaaaag tattcataat tggcctaata taatattgca taatctcagt
     1501 acacgcaaga ccgtgcgagt tcattagcgc ttccttgata gatatgtaca cagaaaggtt
     1561 gtcttagagc agttcttccc ctctcagcta gtatatcagc gagctgttga gccttcgcac
     1621 tatttggggg agaaccgaca gctttcgaag atacctgtgc ccaaagggaa ataggagttt
     1681 tccactgtgc gcttgtaatc ccaggaagcc ctctcactat tgcagtaaag tgtttttgag
     1741 cagctgtaag gtcgtactcc tcaagaatag tgtatctact aaagccaaaa ataagaatcg
     1801 aaataacaaa aatccataat gataatctca tcatcctcct ccgttttaat gtgtgacaat
     1861 aatagtttat acccctttaa ataacttgtg tctagtctca ataaaatgat tgtgaaaagc
     1921 tattatcaaa tatgaattaa tacctgtcaa gccagttctt taatttttat tacctcgcaa
     1981 cccagccccc gtctacagag agcattgtcc cggtcataaa ctcaacatta tcatcgcata
     2041 aaaataaaac ggcatgggcg atttcttctg gtctgccaag gcgtccaatc gggtggaaag
     2101 atttgaaaag ctcttcagcg gcttttctat cccctatttg ttcgaataaa ccttcgacca
     2161 tcggagtcac aatcgttccg ggacagattg catttaccct gattcctttg cttgagtact
     2221 caagagccat gctccttgta agctgtgtaa cagctccttt agtggcatta tatgcagcaa
     2281 caccgggcac cgcttttagt ccgagtacgg aggagttatt tataatacat ccttttccct
     2341 gtttcaacat gtggggtatg gtgtatctgg acataagata agttcccctt gcatttacag
     2401 caaatgtttt atcccatact tcagtcggtg tttcatgcgt cgttcctgta taaaggacgc
     2461 cagcgttgtt aaagagtatg tctatgcggc cgtatttgtt aattgtataa tcaatagcct
     2521 ctttacagtc ctcttctttt gctacgtcac ttacgagata gtcaatcttt agaccctctt
     2581 ttcttgcttc ttctgctaca tgcttcagtg ttttctctgt acgcccagta attacaacct
     2641 ttgcaccttc ttttgcaaaa agaatagcgg ttgcttttcc tattcctata cctccaccgg
     2701 tgataagagc tactttattt ttaagtttca ttcaaataaa ccctcccttt taattgcctt
     2761 caattataaa tcgaaagatc attcaaaatc aagggatgcg cttttatatt gaataagagg
     2821 gaacactaat tttagttatt gccaacttat tatagtaatt tgaaatttaa attatttact
     2881 ctataacagt attgaattta tgttttgtaa tcaagggtat taaataacct ttcacctttt
     2941 aagtttaatt ctgtgtttga aagtaataaa tattggaaag cgattcatct tcctaaagtt
     3001 ggttttgaaa gaaattagcc ttggaaaatc aattgatatt aaaacctcag ttttatataa
     3061 ttactgtaag aaatatatct taacagtcta aatttaaggt aattaaatga ggtataaaaa
     3121 gggatgaaaa aaatcgtaga attttattat gaccttggaa gtccttatag ctatcttgct
     3181 tctacgcaga ttgagaggat ttgtgaaaaa tataatgccg atctagaatg gaagccatta
     3241 ctgctcggag gggtgtataa ggaaacagga aaccgtgctc cgcttgaggt tcctaataaa
     3301 aggcatacat gattaaagac ttagccgact gggcagggta ttatggagtt aggtttaact
     3361 ttcctgattt atttccatta aacggtgtta agccaatgcg tggtgccttg gttgcgaaag
     3421 agaaggaaaa gatcagagac tatacgcata ggctatttga gctatattgg atacatggga
     3481 aagatttaag ccaagatgag gtattagaga aggcagtgag agaacttggt atagatgcgg
     3541 gctggtttat gcaaagaatt ggagagcaag atataaaaga taatctccgg gaagaaacag
     3601 ttaaggctgt aaaacggggt gcgtttggcg cccctacaat ctacgtttgg gataagatgt
     3661 tctggggaaa tgatagattg atctttgttg aagaatatct aaagggaaag ttctaaatat
     3721 cattgctact aagacaccaa ggcacaaagg aggaattcta actttgtgtc ttcgtgccta
     3781 aatagttact atgtaggctg attgatttta ttatgccttc ttggtaaatt tccatcacca
     3841 aagataaagg gagaaaaact aaatgtggta tgactttcaa tacaagggtg gagagtatca
     3901 tgttgaagat gtatctgtaa aagaaattgt cgaggctgtt ggtacacctt gttacatcta
     3961 tagcaattcg gccttagaag gggattttga tgtttttcgt gaagcctttt ccgaaattaa
     4021 ccctttaata tgtttctccg ttaaagcaaa ttccaacctc gccgttctaa aaacttacgc
     4081 aaaacttgga gtaggttttg atgttgtttc gggaggagaa ctactccgtg ttctacgtgc
     4141 tggtggagag ccggaaaagg ttgtgttttc aggggttgga aaaacagaag atgagataaa
     4201 agccgctatc gaggctggca ttttattctt caatgttgaa tctgtagacg agctacaaat
     4261 tattaacgat gtagcaaaac ggttaggtaa gaaggctagg attgcactca gagtaaatcc
     4321 agatattgat cctaagaccc atccatatat ttctacagga ctcatgaaaa gtaagtttgg
     4381 gattgagatt gggaaagcct tagacgttta taaaaatgct tcaaaaatgg aaggaatcga
     4441 agtcatgggg attgacgcac atattggttc acagatattt caactcgagc cctttgtcga
     4501 atcattaaga aagctaattg agcttgggga cgctctgaag aatgaaggca ttaccattca
     4561 atacattgat ataggaggag ggctagggat aagctacaaa gaaggtgaga tgccacccca
     4621 tccaaaggaa tacgcagatg ttataaaaag agaaataagt ggcactcctt ataaactcgt
     4681 gttggagcct ggaagggtgc tcgttgggaa tgcagggatt ctcgtaacta aggtgcttta
     4741 tataaaagat ggagtatcta agaaatttgt agtggtggat gcagcaatga atgatttgat
     4801 tcgtcctgct ttctatgatt cttatcatga gattattact attgatgaga gtctcggaga
     4861 cgtagaaacc gtggacatag ttggacctgt gtgtgaatcg ggagatttct ttgcaaagga
     4921 taggggactt ccaaaggtaa aaaggggtga ccttcttgct atattgagtg caggtgcata
     4981 cggttttgta atgtcttcta attataacac ccgtcctcgt gtcccagagg taatggtaag
     5041 aggagaggag ttttttgtag tacggggaag agagacatac gaagatttat ttaaaggcga
     5101 gattattcca gattttatga agtgaatgaa ccttctggga atgtcgtggg cagaacattt
     5161 aattgctaaa ttaaccaact tgaatattct ggtataatat tttggataat gttatataga
     5221 atagagtcat ttggaggaac aaaacccttc gcctctt
//
LOCUS       DATDRL010000195         4205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_13773, whole genome shotgun sequence.
ACCESSION   DATDRL010000195 DATDRL010000000
VERSION     DATDRL010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13773"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..503)
                     /gene="rpsR"
                     /locus_tag="VI935_06765"
     CDS             complement(261..503)
                     /gene="rpsR"
                     /locus_tag="VI935_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HLE25337.1"
                     /translation="MERENTKEKKYFVRKKVCRFCMEAIEVDYKRVDLLSQFISERKK
                     IVPRRSSGLCADHQRDLASAIKRARVMALLPFTVLH"
     gene            complement(507..863)
                     /gene="rpsF"
                     /locus_tag="VI935_06770"
     CDS             complement(507..863)
                     /gene="rpsF"
                     /locus_tag="VI935_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HLE25338.1"
                     /translation="MIELNYYETLYLTRPDLTEDELGKVHGKLKEAIANHDGEIYKSE
                     KWSERNLAYRIGRFTKGVYYILVYKALPSVVTEVEKNLRFFNTDVLRFMTVRIKKETA
                     LSEKAASEMHLEGGGS"
     gene            complement(935..2023)
                     /gene="ychF"
                     /locus_tag="VI935_06775"
     CDS             complement(935..2023)
                     /gene="ychF"
                     /locus_tag="VI935_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HLE25339.1"
                     /translation="MNLSCGIVGLPNAGKSTIFNALTSAQAHVANYPFTTIDPNVGIV
                     NVPDERLNKLGEIIKPEKIIPATIKIIDIAGLVKGASKGEGLGNRFLAHIREVDAVIH
                     VVRCFDSKVPHIFETIDPKRDVDVINLELLLSDLDTVEKRIVKLEKVLKSGNKEALTE
                     LSLLERLKTHISQGKSARLFITKGDIDEKILGELNLLTIKPVLYVANLEEKEGHGFCI
                     ETLRNIAEEEKAGLIEILGKFETELSEFSEEESEAFLSDMGLTESGLRRLIKAAYKLL
                     NLITFYTTNGIELRAWTVKKGTYAPKAAGKIHTDFEKGFIKAEVISYEDFIRSRSDTV
                     ARESGLLRIEGKDYQIRDGDMVYFRFKI"
     gene            complement(2242..2838)
                     /gene="pth"
                     /locus_tag="VI935_06780"
     CDS             complement(2242..2838)
                     /gene="pth"
                     /locus_tag="VI935_06780"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HLE25340.1"
                     /translation="MILVAGLGNPGKGYASSRHNIGFIVIDELAKRLGASVKKKGFRS
                     HYAQAPIDEKKLILLKPDTYMNRSGEALSEAAEFFKIPAKDIIAVYDEMDLSLGSIKV
                     KVGGGSAGHKGIQSIINSLGDSDFIRVRVGIGKPVQKSEVIDHVLSQFEKEEKKLVKD
                     AIVKAADAVIEIVLMGAESAMNKFNKKPEVTSQPRKCG"
     gene            complement(2969..3637)
                     /locus_tag="VI935_06785"
     CDS             complement(2969..3637)
                     /locus_tag="VI935_06785"
                     /inference="COORDINATES: protein motif:HMM:NF004139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HLE25341.1"
                     /translation="MAQSTLFVLKRNRAGKSVAKKIRKEGAIPAIFYGRKTDPIPLTV
                     NPLDLKKALSTEAGENTLLELHIKGDGDEITKIALLREVQYDHLKSLPIHFDFQEVQM
                     KELITVKVPIRIVGRAQGVHEGGILEEILREIEVECLPTAIPNVIDVDVSQLGISDSI
                     HISDLSLSEGVTVLHEPEETIVTILSPVMEEVKPVAPAEVIAPTEAEEGEAKAETEKE
                     EAEE"
     gene            complement(3654..>4205)
                     /gene="prs"
                     /locus_tag="VI935_06790"
     CDS             complement(3654..>4205)
                     /gene="prs"
                     /locus_tag="VI935_06790"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HLE25342.1"
                     /translation="AGQIQGFFDVPVDHLYAAPVFISHLKKKFNKDEIVVVSPDAGGL
                     ERAGEYAKRLDAKLAVTYKRRTGPNEAKISHVIGEVRGKTVVIVDDMVDTAGTAIQAA
                     QALAREGAKHVTLCCTHGVFSGNAPQKIENSVLEEVIITDTIPPREEIKKSKKIKVLS
                     IAELLGEAIRRIAVGESISTLFT"
BASE COUNT         1056 a          954 c          747 g         1448 t
ORIGIN      
        1 gtttttttag atgtgtcaac ttaagcaaat tctgggatac aagagtaagg gtttgataat
       61 caacacctag ataggcgggg ttttccaacc ccgcttgcca atacgggact agaaagtccc
      121 gtctattgaa cgtcctgtct atcttgctaa attgtcattc gaatgccaca attgcctctc
      181 atcccttaag ttaacactga tggacatagt tctgtcccta cgtaattaca ttcgtgacaa
      241 aaattgattt ggcactggtt ctagtgaagc acagtgaacg gaagaagagc cataacacgt
      301 gccctcttta ttgctgaagc gagatccctc tgatggtctg cgcagagtcc cgaacttctt
      361 cttggcacta ttttctttcg ttcacttata aactgtgaga gaagatcaac ccttttataa
      421 tcgacttcta ttgcttccat acagaatctg caaaccttct ttctcacaaa atactttttt
      481 tcttttgtat tttctctttc catcttttat gaacctcccc cttctaaatg catttctgaa
      541 gcggctttct cacttagggc tgtctccttt tttatcctaa cagtcataaa tctgagcaca
      601 tcggtgttaa agaacctgag gtttttctcc acttcagtta caacgcttgg aagggccttg
      661 taaacaagaa tatagtaaac tccctttgta aaccttccga tcctatatgc aaggtttcgc
      721 tcagaccact tctcagattt ataaatctcc ccatcgtggt ttgctatagc ctcctttagc
      781 tttccgtgta ccttacctag ttcatcctcg gttaggtctg gccgtgtaag gtaaagcgtc
      841 tcataatagt ttagctctat cattaaaata cctcccattt tggactttcc gaccctatgc
      901 ttatgatttg aagcataagc taagagtaac gggtttagat tttaaaccta aaatatacca
      961 tatccccatc ccgaatttga tagtctttgc cttctatcct taagagtcct gattctcttg
     1021 cgactgtgtc agaacgagac cttataaaat cctcatatga tattacctca gctttgataa
     1081 atcccttctc aaaatcggta tggatttttc ctgcggcttt tggagcgtaa gtcccttttt
     1141 ttactgtcca tgctcgaagc tctattccat ttgtcgtgta aaacgttata agattgagta
     1201 gcttgtatgc cgcttttatg agcctcctga gtcccgactc agttagaccc atatcactta
     1261 gaaaggcctc cgattcttct tctgagaatt cagatagttc ggtctcaaat ttgcctagga
     1321 tttcaataag tccggctttt tcctcttctg ctatattcct taatgtttca atacaaaatc
     1381 catgcccttc tttttcttca aggttcgcta cataaagaac tggtttaatc gtgagaaggt
     1441 ttaactcgcc cagaatcttt tcatctatat ctcccttggt tataaatagt ctagccgatt
     1501 ttccctgaga tatatgggtt tttaggcgtt ccaacaaact gagctctgtc agtgcttctt
     1561 tatttccgga ttttaaaacc ttttctagtt ttacaattct tttctcaacg gtgtcaagat
     1621 cggaaagaag gagttcaaga tttattacat ctacatcccg cttagggtca atagtttcaa
     1681 aaatgtgtgg gaccttagag tcaaagcacc ttacaacatg aattacagca tctacctctc
     1741 gaatatgcgc aagaaatcta tttccaagac cttcaccttt acttgcacct tttacgagtc
     1801 ctgcgatgtc aatgatttta attgtggctg ggatgatctt ttcgggtttt ataatctctc
     1861 ccagtttgtt tagtctttca tcaggaacat tgacaattcc aacatttggg tcaatcgttg
     1921 taaaggggta attagctaca tgtgcttgag cggatgttag ggcattaaat atagtagatt
     1981 ttcctgcatt aggcagtccc acaattccac agctaagatt catgatattt tactatcttt
     2041 cttaagagta attattaatc cactgataga catgaattta cactaataga ggtgcgatgc
     2101 atattacagg atgcatgatg caagatgcat gatgaatgat tcatggaact tacatggatc
     2161 tgtaccctgt atcgtgcatc atgtatcctg ttttcattcg agttaatttg tgtcaattgg
     2221 cttcgccgaa atgtccgacc actaaccgca ttttcgtggc tgagatgtta cttcgggttt
     2281 tttattgaac ttattcatag cgctttccgc acccattagg acgatttcta taactgcatc
     2341 tgcagcttta actatcgcgt ctttgactaa ctttttctct tctttttcaa actgtgaaag
     2401 tacatggtca atgacctcag acttttgaac tggttttcct atccctactc tgactcttat
     2461 aaagtcacta tctccaagtg agtttattat ggactggatt cctttatgtc ctgcgcttcc
     2521 tcccccgact tttaccttta tacttccaag tgataggtcc atttcatcat agacagcgat
     2581 tatatctttc gcaggtattt taaaaaattc tgcagcctct gaaagagcct ctccacttcg
     2641 gttcatgtag gtatccggtt ttaggagtat tagtttcttt tcgtcgatgg gtgcttgggc
     2701 gtaatgactt ctaaacccct tcttcttaac acttgcgcca agtcttttcg caagctcatc
     2761 tataactata aagccaatgt tgtgccttga ggaagcgtat ccctttcccg gattcccaag
     2821 tccagcaacc aaaatcatta ttcgtaacta tttagccaca gagcacacgg agcgcacaga
     2881 gagaagaaag aacaaaaatt ttatttttaa aattttttct ctgtattctc tgtgctctct
     2941 gtggctatta aaataatacc ttttcaaact attcctcagc ttcctctttc tcggtttcgg
     3001 ctttggcctc tccctcttca gcctcagtcg gagctatgac ctcggcaggg gcaacgggtt
     3061 ttacttcttc cattacaggt gaaagaatcg ttacgattgt ctcctcaggt tcgtgaagga
     3121 ctgtaactcc ctcggagaga ctcaaatcgc tgatgtgtat tgaatcactt ataccaagtt
     3181 gagatacatc aacatcgata acgttaggaa tggcagtagg aagacattca acctcgattt
     3241 ctctcaatat ttcttcaaga attcctcctt catgaacgcc ttgagcccta cccacaattc
     3301 taataggaac ttttactgtt attagttcct tcatctgaac ctcttgaaag tcaaaatgta
     3361 tagggaggct cttgagatgg tcatattgga cttcgcgaag taaggcgatc tttgttattt
     3421 catctccatc gccttttatg tgaagctcta gaagggtatt ctcaccagct tctgtggaaa
     3481 gggctttttt aagatcaaga gggttaacag ttaatggaat aggatcggtc tttcttccat
     3541 agaatatggc aggaattgct ccttctttcc tgattttttt tgccacactc tttcccgctc
     3601 tatttctctt tagtacaaat aaagtgctct gtgccatgtt taaactcctt aaattaagta
     3661 aatagtgtac ttattgactc tcctacagct atcctcctta ttgcttcgcc gagaagctct
     3721 gcaatgctaa gaaccttaat ttttttactc ttttttattt cttctctggg tggaattgta
     3781 tctgttatga tcacctcttc gagtaccgaa ttttcaatct tttgaggtgc gtttcctgaa
     3841 aacacaccgt gggtacagca gagtgttaca tgctttgcgc cttctctcgc taaggcttga
     3901 gcggcttgga tagctgttcc agcagtgtct accatatcgt caactatgac tactgttttc
     3961 cccctgacct ctccaataac atgcgatatc ttggcttcgt tggggcctgt tctcctttta
     4021 tatgtcacag cgagtttagc atcaagcctc tttgcatatt ctccagctct ttctagcccg
     4081 ccagcatctg gtgatactac gactatttca tctttattga atttcttttt taaatggctt
     4141 atgaaaacgg gagcagcgta aagatgatca acgggaacat cgaaaaaccc ctgaatctgt
     4201 cctgc
//
LOCUS       DATDRL010000196         8038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3736034, whole genome shotgun sequence.
ACCESSION   DATDRL010000196 DATDRL010000000
VERSION     DATDRL010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8038
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3736034"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2504)
                     /locus_tag="VI935_06795"
     CDS             complement(<1..2504)
                     /locus_tag="VI935_06795"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HLE25343.1"
                     /translation="MASDKYGSMEGNGKALLEENSFRINIDAEEEVQNEVQVVVEYKE
                     GVMDIPEETINAFSGDELRARVFYEKYALRDERGKIVEKSPDEMWRRVASEIASPEKT
                     QKLKKEWEEKFYWLLSDFKFIPGGRILFGAGQKRRATLLNCYFMPIKEDSIESIFEWC
                     KEAARTYSFGGGVGTDISVLRPKGSPVNNSAIYSTGAVSFMDLMSTTTGTIGQAGRRG
                     ALMITIGVDHPDVLDFIDIKNDPARSKVKFANISVRVTDEFMKAVEEDKDFELRFENE
                     KVRVRRKIRARDLWKKLVKSAWASAEPGVVFWDAVKKFSPTEYNGMEIHGFNPCSEQA
                     LEDYGNCCLGNVNLSLFVNDKFTDRATIDWENLERALRFSVRFLDNVLDYNFEKHPLN
                     AQKEASQRSRRIGVGFTGFGDMLAKLNLKFDTPEAVEFADKMFEYIKNIVYEASSDFA
                     AEKGSFPAFDLDTHLSRPFIKTLDGRAIEKIKTQGLRNACILTVPPVGSGSILAATTS
                     GIEPIFALSYFRRSKSLSQEEFKVYHPLVGEYMKRFGLKDEKELPSIFVTSHEIKPEM
                     RVRMQAAIQKHIDSCISSTVNLPTDITLEEVEKVYFLSWKHGCKSITVYREGSREGIL
                     VTEEAWKKTSEKNVVSEIEPEVSTRDEGMHHPQYPISSLHPTLSEPFDRAQDRSDRTP
                     MPEIKPLKRPHYLEGFTEVIKTGYGNLYVTVNTYQGRPFEVFVQIGKSGYTTMADAEA
                     IGRLTSLALRCGIGVKDVVEQLEGIGGASPVFSEGKLVMSIPDAIAAVLRKHFMSGIE
                     NGVEAKVKKIDIGLERCPDCGSRALAFEQGCIVCKS"
     gene            3423..3593
                     /locus_tag="VI935_06800"
     CDS             3423..3593
                     /locus_tag="VI935_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25344.1"
                     /translation="MPKKGRKKIKDLVDMLEDPDNTTYIGGDYEDIMEQLEKEISATE
                     EVEENQENKEEE"
     gene            complement(3569..5482)
                     /gene="mnmG"
                     /locus_tag="VI935_06805"
     CDS             complement(3569..5482)
                     /gene="mnmG"
                     /locus_tag="VI935_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HLE25345.1"
                     /translation="MIITNPKRYQVIVIGAGHSGCEAVLAASRMGCKSLMLTANIDTI
                     GLMSCNPSIGGVGKGHLVKEIDALGGEMGRAADATAIQFRRLNTSKGPAVQASRIQAD
                     RQAYKAYMRKALESEENIDIKQRMVEGFLAEGGKVVGVKTNLGEYFLADAVVVTPGTF
                     PNGLIHIGQTRISAGRAGEAPAHGISDSFESLGFKIGRLKTGTPPRLDGRTINWAILE
                     PQPGDSDPKPFSFSNDKIEREQLPCYITYTNEKTHEVIRKNLHKSPLYSGVIKGIGPR
                     YCPSIEDKVVKFADKERHQIFLEPEGIGTYEIYPNGISTSLPLDVQFELVRTIKGLEE
                     VEIIRPGYAVEYDYSDPTQLKPTLETKIVENLFFAGQINGTTGYEEAASQGLIAGINA
                     ALRVKGGEPFILDRSQAYIGILIDDLVTKGVDEPYRMFTSRAEHRLILREDNADLRLR
                     EKGYKIGLVKDEDYERYLSKKTKIQEELERLEHARVFPDSRINEILTQLGSSPLKKPQ
                     TLKELLRRPEITWRELGLIDSYYTSLINKDISAQIEMEVKYEGYIQRQMEQVDKFKKL
                     ENMMIPESFQYDGIPGLSREIVQKLSRIRPGSLGQASRISGITPAAISILMVYLKRRE
                     IEVREGYSSSLFS"
     gene            5585..5953
                     /locus_tag="VI935_06810"
     CDS             5585..5953
                     /locus_tag="VI935_06810"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HLE25346.1"
                     /translation="MVKYGINDLRRSTRKMTIPKEIFEKLQKKIVEKSDEFSQIAAVY
                     KFVLNGPNGGTWIVDLRKDTLGVREDNSEAKCTVSMSDENFVKLVTGELKAETAFMTG
                     KLKLYGDMGLAIKLGKLFGK"
     gene            6070..6492
                     /locus_tag="VI935_06815"
     CDS             6070..6492
                     /locus_tag="VI935_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25347.1"
                     /translation="MMQKPDRLYIEENVKYYPLTEKIICKLAPISVEFVDDYRKIGEA
                     KPFPKKAAEDKNSLALAEKRGEVLKSIGRMVEELFSALDSKKTNYIFLGTIKLHKLLV
                     EAMRERFPKNQILLDELAPSVDGKYKYLKFKRVDVIER"
     gene            complement(6684..7151)
                     /locus_tag="VI935_06820"
     CDS             complement(6684..7151)
                     /locus_tag="VI935_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLE25348.1"
                     /translation="MGLGKLFGFKKGKQEDKEEKQKGAMKLEQAVKKPQSEATKPRYE
                     EEPDPFAGIDGEEAVSSVLSGRFQVVDVRSHYEYQSHHIPGSIHIFIQQLENRYKELD
                     PTRDTLVVCERGIRSQDACWFLSDMGFKQLFHLTGGLSVYKGPQKGTRVGNPD"
     gene            7559..>8038
                     /locus_tag="VI935_06825"
     CDS             7559..>8038
                     /locus_tag="VI935_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HLE25349.1"
                     /translation="MSVFRRIREFKKPTNGSGQGGVRRFPRVIFIYIVLGAWILLSSF
                     YIVDADEQGVVKRFGKYNRTQKPGVHFKAPWPIESVLKPRVTEVKRIEIGFRTIDPGP
                     PASYRHIPEESLMLTGDLNIVSSEFIVQYRIKNAEEYLFNVKDVDTTIRDAAEAAKRQ
                     "
BASE COUNT         2372 a         1710 c         1480 g         2476 t
ORIGIN      
        1 cagcttttgc aaacaatgca gccttgttca aaggcgaggg cgcggctgcc gcagtcaggg
       61 cagcgttcta gacctatgtc aatctttttc accttcgctt cgaccccgtt ttcgatgcca
      121 gacataaagt gttttcttaa aaccgctgcg attgcatcgg gaatagacat gacgagtttt
      181 ccctcagaaa acacgggcga tgctccacct attccttcaa gctgctctac aacatccttt
      241 acgcctattc cacatctcaa cgcaagggat gttagacgtc ctattgcctc tgcgtcagcc
      301 atagttgtat atccagattt tccaatctga acaaatacct caaacggtct tccctggtag
      361 gtatttactg tgacatagag atttccgtag ccagttttta ttacctctgt aaacccttca
      421 aggtagtgag gtcgcttcag tggcttaatc tccggcatgg gagttctatc agacctgtcc
      481 tgagctctgt cgaagggctc actaagggtg ggatgaaggg atgagatggg gtattgggga
      541 tggtgcatcc cttcatcccg ggtactgacc tctggttcga tctcactcac tacatttttt
      601 tcactagttt ttttccaggc ttcctctgtt acaaggatcc cttccctcga gccttctcta
      661 tatacagtta tgctcttaca tccatgcttc caagagagga aataaacctt ctctacctcc
      721 tcaagtgtaa tatcggttgg aagattcacc gtgcttgata tgcatgagtc aatgtgtttt
      781 tgaatcgcag cctgcatacg gaccctcatc tcgggtttta tctcatgcga tgttacaaat
      841 atagagggaa gctccttttc atctttcaaa ccaaatcttt tcatatactc accaacaagg
      901 ggatgatata ccttgaattc ctcttgagaa agcgatttag aacgcctgaa gtatgaaagc
      961 gcaaaaatgg gctcaattcc acttgtagtt gctgcaagaa tagaaccgct tcctacaggt
     1021 ggaaccgtga gaatacatgc gtttctaagc ccctgagttt ttattttttc aattgcgcgc
     1081 ccgtcaaggg tcttgataaa tggacgcgat aaatgggtat ccaggtcgaa tgcagggaag
     1141 cttccttttt ctgctgcaaa gtctgagctt gcttcataaa cgatattctt tatgtactca
     1201 aacatcttat ccgcaaattc aacggcctcg ggtgtgtcga attttaggtt gagctttgca
     1261 agcatatcac caaaaccagt gaacccaaca ccgattcggc gtgagcgctg cgatgcctcc
     1321 ttttgtgcat taagtggatg cttctcaaaa ttgtaatcaa gtacattgtc aagaaaacga
     1381 acagagaacc taagcgcgcg ctcgagattt tcccaatcta tagtagctct atctgtaaat
     1441 ttgtcattta caaaaagtga aaggtttacg ttcccaaggc aacagtttcc atagtcctct
     1501 aatgcctgtt cgctacacgg gttaaagcca tgaatctcca tcccattgta ttcagtaggt
     1561 gagaatttct taactgcgtc ccagaaaaca accccgggtt cggctgatgc ccaggctgat
     1621 tttacgagtt ttttccaaag gtctcgtgca cggatttttc tacgaaccct taccttctca
     1681 ttttcaaacc tgagttcaaa atccttatcc tcttctacag ccttcataaa ctcatctgtg
     1741 actcttacag aaatatttgc aaacttgacc tttgaccttg cggggtcgtt ctttatgtca
     1801 atgaaatcca agacgtcagg atggtcaacc cctattgtta tcatcaaggc tccacgacgc
     1861 cctgcctggc ctattgtccc cgttgtcgta gacataagat ccataaacga cactgcacca
     1921 gtcgaataga tagccgagtt gtttacagga gaccctttag gtctaaggac tgaaatatct
     1981 gttcctactc ctccaccaaa ggaatatgtt ctcgctgctt ctttacacca ttcaaatatt
     2041 gattcaatag aatcctcctt tatgggcata aaatagcagt ttagaagagt agctcttctt
     2101 ttctgacccg ctccaaaaag tatccttcca ccgggaataa atttaaaatc ggaaagtaac
     2161 cagtaaaatt tttcctccca ttccttctta agcttctgtg ttttttccgg agaagcgatc
     2221 tcgcttgcaa ccctgcgcca catctcgtca ggggattttt caactatctt ccctctctca
     2281 tcacgcaaag cgtatttttc gtaaaatacc cttgcacgga gctcatcacc gctaaaggca
     2341 tttattgttt cctccggtat atccatgacc ccctctttat actcaaccac tacttgtacc
     2401 tcgttttgaa cctcctcctc cgcatcgata ttgattctaa agctattttc ttctaataat
     2461 gcctttccat ttccctccat cgatccatac ttatcagaag ccatttgaat ccatcctcct
     2521 ttttatcaca tattgtatta ttacccgatt gaaccacaat atattgtgcc atcaaatttg
     2581 tcaagggggg tttataaatt caaataaaag agttaaatta acaatgataa agtcagattc
     2641 tcgttttttt cgcctcgatt tattcaatac tcaggcataa aaaactaatg atattagata
     2701 gttattaact gttatgaaac tattgactca agattttatt aaaaccatat tacaacactt
     2761 attttttctc tatattataa attaagttag atttagaaac tcctttaaat ttttgatctg
     2821 gccatattat ctcgcataat attaaagcaa aaggatgctt aatacataaa aaaccgccat
     2881 cttccttact tcgcactata ataaaccgtt ctcaagacta ttaatagaaa aagctttcac
     2941 ccgatggcat aaattattac ttcaatttta tcagtctaac gtttttaaat cataaccatc
     3001 aatttcatga aaataactat atagtatatg tttatgattc attcaaaata tatttaggtg
     3061 atttgatttc acattcgaat tattaaatcg aagtttaatc tagaattaag ctttcatgaa
     3121 attataaaga actttttgta ctcttggttt aatttattgt tcatatttcg tttataataa
     3181 tttgattact gagggaaata gtgtcctcga tttttctcaa gatgtaaatt gaaaaaagat
     3241 tagagtagat ttaagttact cataaaatca aacttgcatt ttaaaaactt atacttataa
     3301 tttcattaaa agttaataat gcctaacaat ctcaaggatt tcacattcat ctcagaagat
     3361 ttttaatgct ttgatctacg agcgcgattc atatgatgca tatccaacgg aggtaagaca
     3421 gaatgcctaa aaagggtcga aaaaaaataa aggatttggt tgatatgctc gaagaccctg
     3481 acaacactac atacattggc ggagattatg aggacattat ggagcagtta gagaaggaga
     3541 tttccgctac tgaagaagtc gaagagaatc aagaaaacaa agaagaggaa taaccctccc
     3601 tgacctcgat ttccctacgc ttaaggtaaa ccataagaat agaaatagct gcgggtgtta
     3661 tgcctgatat cctgcttgcc tgtcctagag aacccggacg aatgcgtgag agcttctgta
     3721 cgatttctct tgataaccca ggtataccat catattggaa actttctggt atcatcatat
     3781 tttcaagctt cttaaactta tctacctgtt ccatctgcct ttgaatataa ccttcatatt
     3841 taacctccat ctcaatctga gccgatatat ctttatttat caaagatgta tagtatgaat
     3901 caattaaacc aagctctctc catgtaatct caggacgcct tagaagttct ttaagtgtct
     3961 gcggtttttt aagtggtgaa gaaccaagct gtgtaaggat ttcgttaatt cgtgagtcag
     4021 ggaaaaccct tgcgtgttcg agcctttcaa gctcttcctg aatctttgtc tttttactta
     4081 ggtacctttc ataatcttca tcttttacta gtccaatctt atatcccttt tctcttagcc
     4141 ttaaatccgc gttgtcttct ctaagaatga ggcgatgctc tgcacgcgat gtaaacatcc
     4201 tgtatggttc gtccacccct tttgttacaa ggtcatcaat caatatgccg atatatgctt
     4261 gagaacggtc gagtataaat ggttctcccc cttttacacg aagtgcagcg tttattcctg
     4321 caataaggcc ctgagatgct gcctcttcgt aacctgtagt gccgttaatc tgccccgcaa
     4381 aaaatagatt ctcaacaatc ttggtctcaa gcgtgggctt aagctgagta gggtctgaat
     4441 aatcgtattc aaccgcataa cctggcctta ttatctcaac ctcttcgagt ccttttatcg
     4501 ttcttacaag ctcaaactga acgtctagtg gaaggcttgt tgaaatcccg ttcggataaa
     4561 tctcataagt tcctattcct tcaggttcga gaaaaatctg atgtctttcc ttatctgcaa
     4621 acttaacaac cttatcctca attgatggac agtacctagg tcctatacct ttgataacac
     4681 cggagtaaag aggagatttg tgaagatttt ttctgattac ttcatgggtt ttttcatttg
     4741 tatatgttat atagcaggga agctgttccc tctctatctt atcgtttgaa aacgaaaatg
     4801 gctttgggtc agaatctcca ggttgaggct caagtattgc ccagttaata gttctcccgt
     4861 ctagcctagg tggagttcct gtttttaatc ttccaatttt aaaaccgaga ctctcaaatg
     4921 aatctgaaat tccatgtgcg ggagcttcac ccgcacgccc tgcagaaatc cttgtctgtc
     4981 ctatatgaat aaggccgttc ggaaaagtgc ccggagtcac aacgacagca tccgctagaa
     5041 aatattctcc tagattagtt tttacaccaa ctaccttacc accttctgct agaaatccct
     5101 ccaccattct ctgctttata tctatatttt cctcagactc taatgccttt ctcatgtatg
     5161 ctttatacgc ctgccggtct gcctggattc tcgaagcttg aacagcaggt cctttacttg
     5221 tattgaggcg tctaaattga atagctgttg catccgctgc ccttcccatc tcgcccccaa
     5281 gtgcatctat ttcttttaca agatgccctt tcccaacacc gccgattgag ggattacacg
     5341 acataagccc aatcgtatca atattcgctg taagcataag ggatttacaa cccattcttg
     5401 aggcagcaag cactgcctcg caccctgagt gccctgcgcc gattacaatt acctgatacc
     5461 tctttggatt tgtaataatc atagttctta accttacaaa ataagtttta aaaattttaa
     5521 acttctaaag ggtaatcgtc aattcgctga gttccctata cccaatagaa cgcagatacg
     5581 atgaatggta aaatatggaa taaacgattt aaggagatca acaagaaaaa tgactatacc
     5641 caaagaaatt tttgagaagt tgcagaaaaa aatagtagaa aaatctgatg agttctctca
     5701 gatagctgca gtttataaat tcgtattaaa tgggcccaac ggagggacat ggatagtaga
     5761 tcttagaaaa gatacgcttg gtgtaagaga agataatagc gaagctaaat gtactgtgag
     5821 catgtcagat gagaattttg tcaagttagt tactggagag ttaaaagcag agacagcatt
     5881 tatgacagga aagctaaaac tatatggaga tatgggactt gctataaaac tcggaaagct
     5941 atttggaaaa taaaacttta gccacagaga gcacagagaa taaggaggaa agacttaaaa
     6001 aaattaaatt tttgtttttt tcttttctct atgcgctctt tgtcctccgt ggctaattac
     6061 aatcaaaaaa tgatgcaaaa acccgatagg ctttacatag aagaaaatgt aaaatattac
     6121 cctcttactg agaagattat atgcaaattg gctcccattt cagttgagtt tgttgacgac
     6181 tacagaaaaa tcggagaggc aaagcctttt cctaaaaaag cagcagagga taaaaactcc
     6241 ctggccttag cagagaaaag gggagaggtt ttaaaaagca taggaagaat ggtcgaagaa
     6301 cttttttcag ctctcgattc aaaaaaaacc aattacatat ttcttggaac aattaagctt
     6361 cataaactac ttgttgaagc aatgagggaa cgatttccaa aaaatcagat tcttcttgac
     6421 gaactggctc cttcggtcga tggaaaatat aaatacctga aattcaagag ggttgatgtt
     6481 atagaaagat gatttcatgg atatggcgat taaataattc tttagagatt aagctatcta
     6541 tggaatccag gaaagcaaaa gatttggttt ttaatgctcc ctatgcataa ggtcaactag
     6601 ttgtaaaccg ccataaaaga aaattagtaa ctaacatgag tcagaagtta cttatttcaa
     6661 cagcgacaat ttttactcct atcttaatct ggattcccaa cccttgtccc cttctgtgga
     6721 cccttataca cactcagtcc tccagttagg tgaaaaagct gcttaaatcc catatcacta
     6781 agaaaccagc aagcgtcctg actacggatg ccgcgctcgc agacaactag ggtatcacga
     6841 gttggatcga gttccttgta tctattctct aattgttgta taaaaatgtg tattgagccc
     6901 ggtatatgat gagactggta ttcataatgg cttcttacat ccacaacctg aaatcttcca
     6961 gatagcacgc tagaaaccgc ttcttctcca tctatacctg caaacggatc aggctcttcc
     7021 tcatatctag gcttagttgc ctcactttgg ggtttcttca ccgcctgttc taatttcatc
     7081 gccccttttt gtttttcttc tttgtcttct tgtttaccct ttttaaatcc aaaaagcttt
     7141 ccgagtccca tgccttatct ccataattcc gtcattagcc taaatttagc tttgatagaa
     7201 tagcataaaa atggataatt agtatgaact ctatgtaatt aattatatgc ctctattttt
     7261 atttacatta ccctactttt gtgatatgaa taacagatgt tacgcactta tacgctcaag
     7321 gggcaatatg ttgggctggt gcttggtgaa ggtcgtcatt cccacatgtt ttaagcggga
     7381 atcaaaaaca tttatggata cccgatatag acactcgggt atgacaatgg agggatgtcc
     7441 gataaatctt gtccccgcat gtcactcggt taaagcattc gagtgcaagc tttagcgggg
     7501 aacgttcggg catgaaaaag cgtaaaatat gcgcaaggtg agggaataaa aaaggacaat
     7561 gagcgttttt agaaggatta gagaatttaa gaagccaact aatggatccg gtcagggcgg
     7621 tgtgagaagg tttccgagag taatattcat ttatatagtt ttaggagcct ggattctact
     7681 ctcaagcttt tacatagtag acgctgatga acaaggcgta gtaaaaagat tcggaaagta
     7741 taatagaact cagaagccgg gcgtacactt taaggctcct tggccaattg agtcagttct
     7801 aaaacccagg gtaacagagg tgaaaagaat agaaatcggc tttagaacaa ttgatccagg
     7861 gcctccggca agttatagac acattccaga agagtcatta atgctcacag gtgacttaaa
     7921 catagtcagc tctgagttca tagtacagta tagaattaaa aacgcagagg aatatctatt
     7981 taatgttaaa gatgtggata cgactatacg agatgcagcg gaggctgcga agagacag
//
LOCUS       DATDRL010000197         3589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2413703, whole genome shotgun sequence.
ACCESSION   DATDRL010000197 DATDRL010000000
VERSION     DATDRL010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2413703"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VI935_06830"
     CDS             complement(<1..479)
                     /locus_tag="VI935_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HLE25350.1"
                     /translation="MEEWKYWIGLKMVQGVGEVLFRNLLAKFPNPKSIFEANLSELER
                     VEGIGEKIAKEIKSFKDWNKADEEVRKVKKFGFKLLLLKDSNYPRNLFNTYNPPPFLY
                     IKGDILPQDNNAVAIVGTRIPDRYGRFVAERLAEELAVRGVNIVSGMARGIDSIAHIT
                     "
     gene            complement(533..826)
                     /locus_tag="VI935_06835"
     CDS             complement(533..826)
                     /locus_tag="VI935_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HLE25351.1"
                     /translation="MIVELSIIPIGVGTSLSDYVAEVMKIIQESRLKYESHSMGTNIE
                     GEWDEIMPLIKKCHEGLYQKGVQRISTILRISDRRDKPYTMEGKMESLEIKLR"
     gene            complement(939..1865)
                     /locus_tag="VI935_06840"
     CDS             complement(939..1865)
                     /locus_tag="VI935_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HLE25352.1"
                     /translation="MDRRYNNYNNYLKEKFGCRVHKVSVDVGFTCPNRDGKVTIGGCI
                     YCNNDSFVPSYARSSLSLQDQIRTGMEYLKKRFKAEKFIIYFQAYTNTYDKIEKLEKL
                     YKEALDNENVVGLAIGTRSDCVDEEKIKLFEDLAKDWFVTIEYGIESIYDKTLEYMNR
                     GHNYQSLLEAIQITRKQGINIGAHIIVGFPTETRDEMLQMADEISRLNIDFLKVHNLH
                     IVKNTPLARMYAKEPFYIFNYEEYIDFISRFIEQLSPKIVLERLFTDTPQDLLIAPRW
                     NKTHAEILRGIEMELESRNTYQGRLFKEQPYS"
     gene            complement(1940..2614)
                     /gene="nth"
                     /locus_tag="VI935_06845"
     CDS             complement(1940..2614)
                     /gene="nth"
                     /locus_tag="VI935_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HLE25353.1"
                     /translation="MKQEQINPQDENLNSKKKRVKDIISIFRKEYPNPKCHLNFKTPL
                     ELLVATILSAQCTDERVNQVTKDLFRKYKTVMEYAKANQNEFENDIRSTGFYRNKAKA
                     IINCCKTLIERYDGNVPSLMERLTEVDGVGRKTANVVLGNAFGIPGIVVDTHVKRLAK
                     RIGFSDKSDPDRIEADLMELVHKEEWIYFSHALSDHGRQICHARRPLCPSCKINHLCP
                     SAELQR"
     gene            complement(2887..3156)
                     /locus_tag="VI935_06850"
     CDS             complement(2887..3156)
                     /locus_tag="VI935_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HLE25354.1"
                     /translation="MAYIIAEPCIGVKDRSCVDACPVDCIYEVEDQLLIHPDECIDCG
                     ACVDPCPVDAIFHEDELPDKWKSFIEKNKSYFVGKTGLTPAQRKG"
     gene            complement(3240..>3589)
                     /locus_tag="VI935_06855"
     CDS             complement(3240..>3589)
                     /locus_tag="VI935_06855"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE25355.1"
                     /translation="IKIDPAYLNAHISLGRLYVRSGFYEGAIRELEEAVRINPNSPEA
                     HAAMGEALYTIGAGREVKVEHPSGMGATSDFRGAKEYFKKAIDEFNQAIEIEPSLAQK
                     LQPLIEKASQRVK"
BASE COUNT          999 a          751 c          578 g         1261 t
ORIGIN      
        1 gtgatatggg caattgaatc tatacctcgt gccattccac tcacgatatt aacacctcta
       61 acggcgagct cttccgcaag cctctctgca acaaatcttc cgtaacggtc tggaattcta
      121 gtaccaacaa tcgcaactgc attattgtct tgaggaagaa tatcaccttt gatgtaaaga
      181 aaaggaggag ggttgtacgt attaaaaaga tttcttggat agtttgaatc cttaagaagc
      241 aataatttga aaccaaactt tttaaccttt ctaacctctt catcagcctt attccaatct
      301 ttgaagctct ttatttcctt ggctattttt tctccgattc cctctaccct ttcaagttcg
      361 cttaaattgg cttcaaatat acttttaggg ttaggaaatt tagctagaag atttctaaat
      421 agaacctctc ctacaccctg aaccattttg agtccaatcc agtatttcca ttcttccatg
      481 acggtattaa gttttaaccc ctgattttga gtaaagtcaa gaacatgtat cgttatctaa
      541 gctttatctc taaactttcc atctttccct ccatcgtgta gggcttgtct cttctgtcgc
      601 taattctaag aatcgtactt attctctgaa cacctttttg gtaaagccct tcatggcatt
      661 ttttgataag aggcataatc tcatcccatt cgccttcgat gtttgtcccc attgagtgcg
      721 attcgtattt caatctgctt tcctgaataa tcttcataac ctctgctacg tagtctgaaa
      781 gtgaggttcc aacacctatt gggattatgc tgagttcaac gatcatgatt ttttcctcct
      841 ataaattcta taataaacac atagagccca tcgtatctta taatcggtag ttaataaact
      901 gatgtattta ccttttatgc tttagacgga acgggttctc agctataagg ttgttcttta
      961 aataatcttc cctgatatgt atttctactt tcaagctcca tctcaatccc acgtaggatc
     1021 tcagcatgag ttttattcca tcttggagct attagaagat cctgtggagt gtcagtaaat
     1081 agcctttcta atacgatctt aggggacaac tgttcgatga agcgtgagat gaaatctata
     1141 tactcttcgt agttaaatat gtaaaagggc tctttggcgt acattctagc aagaggtgtg
     1201 ttttttacga tgtgaagatt atgaaccttt agaaaatcta tatttaatct tgatatctcg
     1261 tcagccatct gaagcatttc gtctcgagtc tcagtaggaa atccgacgat tatatgagct
     1321 ccaatattaa ttccctgctt tcttgtaatc tggattgctt ctaataaaga ttgatagtta
     1381 tgtcctctat tcatatactc caatgtctta tcataaattg attcaatacc atattctatc
     1441 gtaacaaacc agtccttagc caagtcctca aataatttaa tcttttcctc gtcaacacag
     1501 tctgaccttg tacctatagc aagcccaacg acattttcat tgtctagtgc ctctttatag
     1561 agtttttcta atttctcgat tttatcatag gtgtttgtgt atgcttgaaa atagattatg
     1621 aatttttcgg ctttgaatct cttcttgaga tattccatcc cggttctaat ttggtcctgg
     1681 agggaaaggc ttgatcttgc ataagaagga acaaagctat cattattaca atagatgcat
     1741 cctccaatag tcactttgcc gtctctgttt gggcaggtaa atccaacatc aaccgaaacc
     1801 ttatgaaccc ggcatccaaa tttttctttt aggtaattgt tataattatt gtaacgtcta
     1861 tccatttagt ttagcattct taaaaatctt gtaatttgat aagcttgatt aactttcaac
     1921 ttattaatgc actttcacgt tatctttgca actcagcaga ggggcagaga tggttaatct
     1981 tacaacttgg acataggggt cttcttgcat gacatatctg gcgaccatga tcgctaaggg
     2041 catgagaaaa gtatatccat tcctctttat gaactaactc catgaggtca gcctctatcc
     2101 tatccgggtc ggatttatct gaaaatccga tcctttttgc gagcctcttt acatgtgtat
     2161 ctactacgat tccagggatt ccaaatgcat ttcctagaac aacgttagct gtctttcttc
     2221 ctacaccgtc gacctctgtt aatctttcca ttaatgaggg tacgttacca tcatatctct
     2281 caattaatgt cttgcagcag tttataatcg cctttgcttt atttctatag aacccagttg
     2341 agcgaatatc attctcaaac tcattttgat ttgcttttgc atactccata actgttttat
     2401 atttcctgaa aagatccttt gtaacttgat tgaccctctc gtccgtgcat tgagcagaga
     2461 gaattgttgc tacaagaagc tctagtgggg ttttgaaatt caaatggcat ttaggattcg
     2521 gatattcctt acgaaatatc gaaattatgt cctttaccct cttcttttta gaatttaaat
     2581 tctcatcctg aggatttatt tgttcttgct tcaataccta tgctccgttt ttagttatcc
     2641 atataaaact gaggggctta atgaaaaata catagcttaa taaattgctt attggggatt
     2701 ttataagtaa ttataatact atttgatgat tcttggtaga tacttggata agtgagttga
     2761 caaataaaaa agggatatgt ctcaacaaac atatccctta ccaaatattg ttaatttaac
     2821 tggattaaac ttaactattt tagatcaaaa gatcggtact aagaaagtaa taaagtcctt
     2881 ataaatctaa cctttacgct gtgcaggcgt aagtccagtt ttaccgacaa aatagctctt
     2941 attcttttct ataaagctct tccatttatc tggaagctca tcttcatgaa atatagcatc
     3001 tacaggacag ggatcaacgc atgcgccaca gtcaatacat tcatctggat gtataagaag
     3061 ttgatcttcc acctcataaa tacagtcaac aggacaggca tcaacgcagg atctatcctt
     3121 aacgcctata catggctctg caattatgta tgccattaat tttacctcct taatctttaa
     3181 gttaattata tataaccagt cctaaatatg tcaagttata gaggagtcct aaatattcgt
     3241 tactttactc tctgacttgc cttttcgata agtggttgca gcttttgagc taacgatggc
     3301 tcaatttcta ttgcttgatt aaactcatct attgcttttt tgaagtattc cttagctccc
     3361 ctaaaatcgc tagtcgctcc catacctgac gggtgctcaa ccttaacctc tctgccagct
     3421 cctattgtat ataatgcttc tcccatagcg gcgtgagcct ctggtgaatt tggatttatc
     3481 ctaacggcct cttctagctc tctaattgcc ccctcataaa aaccgcttct tacatatagc
     3541 ctccccaaac taatgtgggc attgagataa gcaggatcta tctttatgg
//
LOCUS       DATDRL010000198         3491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_535642, whole genome shotgun sequence.
ACCESSION   DATDRL010000198 DATDRL010000000
VERSION     DATDRL010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535642"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /locus_tag="VI935_06860"
     CDS             <1..1430
                     /locus_tag="VI935_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLE25356.1"
                     /translation="IALENNRDIQIVKKTVEISVGDIETQKGIFDPQIGLSSSYTDGE
                     IATVSSFIPSGTITQGEFLTQTGVIGTLPTGTFYDLFNFSVSRATTDSNIVDLTPSWN
                     TNLGFTIGQNLLQDFGLDVNLTPITVAKISSEISVKELETTISDVFLNVETAYWNLVA
                     AKKNLELANTTLDLSKDLQRRNEIQVEVGVLPPVAVTEAKSEVARNEVGVIIADNALK
                     AAEDNLKNILAISITQKIDTTDEPTTVVNTYDENQVLNEALEKRPEVAQAKLDIESKE
                     TLKKFYSNQRLPSLSVQASVQLQGLGGSANPDRLSLGDEPEPIPPQFDEASDAFRQLA
                     EVDFPTVAVLGILSFPIFNWTAKGNYVKASADLDRSVITFKKVGDDVELDVSNAIRAI
                     DNSIKGINATKIAVELAEEVLRNEQERLNVGIGTTRNVLDAQRDLVDARFAQIQAIVD
                     YNVALARLERAKGTILEAKGVKIKE"
     gene            1561..2322
                     /locus_tag="VI935_06865"
     CDS             1561..2322
                     /locus_tag="VI935_06865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLE25357.1"
                     /translation="MRLKGKTALITGGGEGIGKATALLFCNEGAKVGITGRKKEKLDE
                     VIKEAEGGGEIIALPGDVSKEEDVKKTVQDFVNKFKKIDILFNNAGVLETGTVTTTTL
                     ESWDKIIGINVKGMFLMCKYVIPHMLKNRGGSIINNSSVAGLIGCPNTVAYCTSKGAV
                     MQFTRCLALDHVKEGIRVNTICPGFIRTKMNEDFISNPPDAQKQLDEMAAQIIPMGKR
                     GEPIDIAYGVLYLASDESKYVTGSSLVIDGGWTTY"
     gene            2596..3474
                     /locus_tag="VI935_06870"
     CDS             2596..3474
                     /locus_tag="VI935_06870"
                     /inference="COORDINATES: protein motif:HMM:NF017807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLE25358.1"
                     /translation="MALNIFLLLFVVITWAYSWVLMKIGLQFMEPFTFATLRCAIGTL
                     GILPLLYMKRISMPKGEKWFDYLVIGLFQTTGMFGFMLYGMKFVTAGKAAVLLYTMSL
                     WTSLLAHFYLKEKLSALRWLGIISGIIGVLCILGWDTLVNQNAKILFGEFLIIMGAVS
                     WAIANIWDKKRMMNEDTYMVNGLQMLIGTVGLALIAFPTEGLGHVRWTWVSISILIFT
                     GIVASSIDFTLWFYLLRKLDTFTVAFSVMLVPVFGLFLDWLQLGSKLDTGIIIGGGFI
                     LIGIYLETKHQKSKLK"
BASE COUNT         1146 a          575 c          785 g          985 t
ORIGIN      
        1 gaattgctct tgaaaataat agagatatac aaatagtgaa aaaaactgta gaaatttcag
       61 ttggagatat agagacacag aagggtatat tcgacccaca aatagggctt agttcttcct
      121 atacagatgg cgaaatcgca accgtaagct cttttatccc aagtggtact ataactcaag
      181 gagaatttct aactcaaacc ggtgtaatag gaactctacc aaccggtacc ttttacgacc
      241 tttttaattt ctctgtatca agagctacaa cagactctaa tatcgttgat ttaactccaa
      301 gctggaatac aaaccttggc tttacaattg gacagaatct tcttcaggac tttgggcttg
      361 atgtgaatct aactcctata actgtagcaa agatatcaag tgaaattagt gtaaaagagc
      421 ttgaaacgac tatttctgat gtttttttga atgtagaaac agcttattgg aatcttgttg
      481 ctgcgaaaaa gaatctagag ttggcaaaca ctacactcga tctttcaaaa gacctgcaaa
      541 gacggaatga gattcaggtg gaggttggtg tacttcctcc cgtcgcagtt acagaggcaa
      601 aatcagaggt tgcaagaaat gaggtgggtg taataatagc tgataatgca cttaaagcag
      661 cagaggataa ccttaagaat attcttgcaa tatccattac ccaaaaaatt gacaccactg
      721 atgaaccaac aacagttgtt aatacatacg atgaaaacca agtgcttaac gaggcgttag
      781 aaaaaagacc ggaggtagct caggcaaagc tcgatataga aagtaaggaa accttgaaga
      841 agttttattc aaaccagagg cttcctagct tatctgttca ggcatcggtt cagcttcagg
      901 gtttgggtgg aagtgcaaat ccagaccgac taagcttagg agacgaacct gagccaatcc
      961 ccccccagtt tgatgaagct tcagatgcat ttagacagct ggctgaggta gactttccga
     1021 cggttgccgt tctaggtatc ctaagctttc ccatttttaa ctggactgcc aaaggaaact
     1081 atgtaaaagc ctcagctgat cttgatagga gcgttataac ttttaaaaag gttggtgatg
     1141 acgttgaatt ggatgtaagt aatgctataa gagcgataga taatagtatc aagggtataa
     1201 acgccacaaa aattgctgtg gagcttgctg aggaagtact gagaaatgaa caagaaaggc
     1261 ttaatgtcgg aattggtaca acaagaaatg tattggatgc tcagagagac cttgtagatg
     1321 caagatttgc acagatacaa gcaattgtgg attataacgt tgctcttgcg cgacttgaac
     1381 gtgcaaaagg aactatttta gaggcaaaag gcgtgaagat aaaggagtag gaaataagaa
     1441 tataagacat attgattatt ccgaatgttg gataacttcc tgtcaacaaa tttgatgttg
     1501 agttatcaat taacataata accattatga gtacgagagc ataagaggag gtaaaaagta
     1561 atgagactca aaggaaaaac agcgctaatt actggaggag gtgaagggat tggtaaagcc
     1621 accgcacttc ttttctgtaa cgaaggagct aaggtaggga tcacaggaag aaaaaaagaa
     1681 aaacttgatg aagttataaa ggaagcagaa gggggcggag aaattatcgc actccccgga
     1741 gatgtgtcta aggaagaaga tgtaaaaaaa acagttcaag attttgttaa taaatttaag
     1801 aagattgaca tactgtttaa taacgcaggc gtcctcgaaa caggaaccgt aacaacaacc
     1861 acactcgagt cgtgggataa gataataggc atcaatgtaa aaggcatgtt tcttatgtgt
     1921 aaatacgtaa ttccacacat gttaaagaac aggggtggtt ctatcattaa taactcctct
     1981 gttgctgggt taattggttg tccgaatact gttgcgtatt gcacttcaaa aggagcggta
     2041 atgcaattta ctaggtgcct tgcgttagac catgtaaagg aagggatacg tgtaaatacg
     2101 atttgccctg gttttataag gacaaagatg aatgaagact tcatcagcaa tccaccagat
     2161 gcccagaaac agctagatga aatggcggca caaatcatcc ctatgggaaa gcgaggagaa
     2221 cctattgata tagcttatgg agttctttat ctagcaagcg atgagtccaa atacgttaca
     2281 ggcagtagcc ttgtaataga tggtggttgg acaacatact aagtttgtaa aagaaaagta
     2341 agtaaaagaa taacaaaact taattctata tggttgatcc attaccataa attggtggaa
     2401 atttggatga ttaagagctg tagggcacgt tccctgaacg tgccaagcaa aaagattaaa
     2461 gcccgtttgg gaaacgggca ctacaggtat tcggtagata ttagcttcta acttttaaga
     2521 cccccatctg ttaaaagtat gaggtcaagc tgtaatgcgg attctgatga atctgattaa
     2581 aggagttttc tcgaagtggc actaaacatc tttttgttgc ttttcgtggt tattacgtgg
     2641 gcgtattctt gggtattaat gaagatcggt ttgcaattta tggagccctt tacatttgcc
     2701 actctgcgat gtgcaatcgg tactttagga attcttcccc tcttatatat gaaaaggatt
     2761 tctatgccga aaggggaaaa atggtttgat tatctggtga ttggactttt tcaaaccaca
     2821 ggtatgtttg gatttatgtt gtatggaatg aaattcgtta ctgcaggaaa agcagcagtt
     2881 ctcctttata cgatgtctct atggacgagt ctgcttgcgc acttttatct taaagaaaag
     2941 ctaagtgcat tgaggtggct aggaattatt agcggtatta taggggtcct ttgtatccta
     3001 ggatgggata ccctggtaaa tcaaaatgct aagattctat ttggagaatt tttgatcatt
     3061 atgggagcag tttcatgggc tattgccaat atctgggaca aaaaaaggat gatgaatgaa
     3121 gatacataca tggtaaacgg tctacaaatg cttattggaa ccgtaggcct tgctctgata
     3181 gcatttccaa ccgaggggtt agggcatgtt agatggacat gggtatctat aagcatcctc
     3241 atatttacag gaatagttgc ttcttctata gactttaccc tatggttcta tctgttaaga
     3301 aagcttgata ccttcaccgt tgcattctcc gtaatgctag ttccagtatt tgggctattt
     3361 cttgactggc tacaattagg cagtaaatta gatacaggga ttatcattgg aggtggattt
     3421 atcttaattg gaatttatct agaaactaag catcaaaaat ctaaactaaa gtaaaattat
     3481 atttgttgcc t
//
LOCUS       DATDRL010000199         7032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3393460, whole genome shotgun sequence.
ACCESSION   DATDRL010000199 DATDRL010000000
VERSION     DATDRL010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3393460"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VI935_06875"
     CDS             <1..295
                     /locus_tag="VI935_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25359.1"
                     /translation="SLKAWTPFLANLALAPLAILAGLLPLTLAGIGTRDAALIALYQP
                     FFNAPTGAALGLLCTSRYLLPAICGLPLLRKYIQMAHTSRKKPGKLWGLGTEL"
     gene            596..2263
                     /locus_tag="VI935_06880"
     CDS             596..2263
                     /locus_tag="VI935_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25360.1"
                     /translation="MFTNAIHTFNEFPIWNPYFGGGIPWAGMVWNPGISPMSLILILF
                     GEVIGIKLWLFCTYLFGAIGMYLMCHNCLNTSRPAASLAGMLFVGSLWAAGRFISGNY
                     LEFTFLLLPLCIYLLNCVISFKLIGIFLPIIYLFALGAFGKYEIFLMMPFILFITIAY
                     RKENRLSIGQILIGFILTFIVFIGLSLHKLLPLLEIINSNIAEFRVPKTGLRALEIIK
                     MIVEVTRLKSINDIIGVGVGGVLLVFIAFLLNWRKSLGLALVLIAAFLLTMGPNTPLF
                     HVVNSIPILDTIKSYPKYFNGFILFSLCALTSVGIDCVLPLVSSRPTERSIDRWQWLT
                     IFLIFSFIVLPPSLTSFRTYKNGFRLKPFKSYEGDFFHVSEERLIGSRRKYRTPNMKN
                     IDTIMYYNLLRNRGTITWYGNFILPENAAPKYIVNHNGNVVRNEDYKGEIYCPKSTRE
                     ACDINNYRFTFNTISLELGKEFSANRVVFNFNFHPGWSSKDAKVVNANGLLAVDLNDN
                     SRTSNIIYLRFVDRRFNVGVKIFLVTFIGWLIGYFALCKIKSTSGDK"
     gene            2997..3326
                     /locus_tag="VI935_06885"
     CDS             2997..3326
                     /locus_tag="VI935_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25361.1"
                     /translation="MQYSSLILHGLSSISVYIDIVGIRVIIFSILWMLPALAGVVNAF
                     SLRLFTNLASPGWASTLSIGLTTIFMLSGIISLLVVLFILSYRTQKQFLPKRDYIDYM
                     LDFSVLK"
     gene            3434..4597
                     /locus_tag="VI935_06890"
     CDS             3434..4597
                     /locus_tag="VI935_06890"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HLE25362.1"
                     /translation="MSHYNTIYNQILHLIPRHQFESIVNKYEGDLYVKYFSCWQQLIT
                     LLFAQIRGKDSLREIETSLRTQSNKWYHIGLKDIKRSTLSDANKHRDWRVYEELFYRI
                     LGRCKDVTPKHKFRFNNPLYTLDATVVELCLSLYPWARFRKRKGALKIHNLYDHSGCI
                     PTFMTVTDARQHDVKVAKKLDLPLMADSIVSVDRAYIDYKWLNSLNTSGVYFVTRAKS
                     NINYEIIGQHEVNREKGLLFDYTIRLRGYYQSRFYPEKLRLVGFYDAGTNKRLVFLTN
                     NFLLSAYTITQIFKARWKIEEFFRWIKQNLKIKSFLGTSKNAVLTQIWIAMCYYLLLS
                     YIKYRTKYSYTLSEFAIMLKETVLERISLIDLLSCTHYNCYKAREPDLQLILF"
     gene            4924..6063
                     /locus_tag="VI935_06895"
     CDS             4924..6063
                     /locus_tag="VI935_06895"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HLE25363.1"
                     /translation="MVKLERIGIIGGGQLARMTAYAAYRMGLEIAILDPEPDSPAGQI
                     AHIKLVGSLNDVPMLEELARVSNLITLENEFVDAPLLEHLESIGTCVFPTARTVGLIQ
                     DKLLQKQILETNGIGVPHFMGVTNYEDIYIAGERFCWPLLLKTRRNGYDGRGNVLIRE
                     PNDTLLAWKTLEGRKSSLMVESFVPFIKELAVMVARSRNREILTYPVVETIQKDHICH
                     IVKVPADVGEDVSKMVATIARQAIEAVDGVGIFGIEMFILEDKSVLINELAPRPHNSG
                     HYTIDACITSQYENHLRAILGLSLGPPTMIQPSAVMVNLLGNREGIAQVSGMENALSI
                     PGVNLHIYGKRLTHLGRKMGHVTALGQNIEEALEKATRAASFLSF"
     gene            6211..6699
                     /gene="purE"
                     /locus_tag="VI935_06900"
     CDS             6211..6699
                     /gene="purE"
                     /locus_tag="VI935_06900"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HLE25364.1"
                     /translation="MGSDSDLPTMKAAAEVCEEFGITYEIRILSAHRTPLDMASYAQE
                     AHKRGLRVIIAGAGGSAHLPGMIAAHTPLPVIGVPIRTEALQGLDSLMSIVQMPPGVP
                     VATVAIGGAKNAALLAIQILATSDKSILQRVISYKEKLADTSREKNKQLKTSKLRERK
                     KK"
BASE COUNT         2173 a         1303 c         1384 g         2172 t
ORIGIN      
        1 ttctcttaaa gcatggactc cctttctcgc aaatctcgca ttggctccgc ttgcaatctt
       61 agcgggttta cttccactga ccttagccgg aatcggcaca cgtgacgccg cacttattgc
      121 cctgtaccaa ccttttttca acgctccgac cggtgccgct ctgggtctgc tttgcacatc
      181 acgctattta ctgccagcga tttgcggact acctcttctc agaaaatata tacaaatggc
      241 ccatacttcc agaaaaaagc ctggaaaact ttggggatta ggtactgaat tgtaaaagtt
      301 aattcatctg gtcaataaaa aaatgatata ttaacgacta ttattatcca gtccgcttcg
      361 cacactccga agccttgttt gtaagcaata gcgcttcaat aaaaatcgca ttttgtaggt
      421 ttatgtttcc atataaatga taaccaagaa tttattaaag cctatttcct attatttccc
      481 gattttcatt ttaatatcat ttgtcttaat gctgactttt gttttttggc tacacccttt
      541 aaaccttcct agcacatttg acccagatgc tttgcagcac cttgcgttcg gctacgtgtt
      601 cactaatgcc attcacacat ttaacgaatt cccgatttgg aacccctatt ttggcggtgg
      661 tataccatgg gctggaatgg tttggaaccc tggtatttct ccgatgtctt tgatattaat
      721 tctattcgga gaggtaatcg gcataaagtt atggctcttt tgtacctatc tatttggggc
      781 aataggaatg tatttgatgt gccataattg cttaaataca tcacgtccag cggcatccct
      841 ggctggtatg ctttttgttg gatctctttg ggctgctgga cgttttatat ccggaaatta
      901 cttggaattc acctttctac ttttacctct ttgcatctat ctcttaaatt gcgtaattag
      961 tttcaaactt attggcatat ttttgccgat tatatacttg tttgcacttg gtgcttttgg
     1021 taagtacgaa atttttttaa tgatgccatt cattcttttt attactattg catatagaaa
     1081 agagaacagg ttatcaatag gtcaaatact aattggattc attttaacgt ttatagtttt
     1141 tatcgggctc agtttgcaca agctcttgcc cttgttagaa attattaact caaacatcgc
     1201 tgaattcaga gtccctaaaa cagggttaag ggctttagaa ataattaaaa tgatcgtgga
     1261 agttacgaga ttgaaatcta ttaatgatat tataggagtt ggagtaggtg gagtgctttt
     1321 ggtatttatt gcttttttac taaattggag aaaatcttta ggccttgccc tagtattaat
     1381 tgccgctttt ttactcacta tgggtcctaa cactccctta tttcatgtgg tcaattcaat
     1441 tccaatcctt gatacaataa aaagttatcc taagtacttt aacggtttca tcttattttc
     1501 gctttgtgcg ctcacttctg tgggaatcga ttgcgttctt ccactagtaa gctcacgccc
     1561 tacggaacga agcatcgatc gatggcaatg gttaaccatt tttttgattt tttctttcat
     1621 cgttttaccg ccttccctaa cgtccttcag aacatataaa aatggtttca gactaaaacc
     1681 ttttaaaagt tatgaaggag atttttttca cgtttctgaa gagaggctta ttggatccag
     1741 acgcaaatat cgaacaccta acatgaaaaa tatagatacg atcatgtact ataaccttct
     1801 cagaaacagg ggaacaataa cttggtatgg aaatttcatt ttgccagaaa atgctgcacc
     1861 taaatatata gtcaatcata atggaaacgt agttaggaac gaagattata aaggcgaaat
     1921 ttattgccca aaatcaacca gagaggcttg cgatattaat aactatcgat ttacatttaa
     1981 cacaatatcc ctagaactgg gtaaggaatt ctcagcaaac cgtgtcgttt tcaatttcaa
     2041 ttttcaccca ggatggtcta gcaaagatgc aaaagtagtt aacgccaatg gcctcttggc
     2101 agtcgattta aatgataatt ccagaacttc aaatataata tacctgcgat ttgtagacag
     2161 gcgatttaat gtaggtgtca agattttcct agtaacattc attgggtggt taattggtta
     2221 tttcgcttta tgcaagatca aatccactag tggtgataaa tgaatggaaa tcagtatttt
     2281 aaagttccta gttagatgct acctcattca cttaggtatt aaagtacatt aaaaaaacta
     2341 cttgctattt tttattattt aagaatatga ttagcgattc aaatttaatt attctaatac
     2401 cagtttataa cgactgggaa cctttattgg ctttactaga aaaaatcaat tcaatggtac
     2461 ctgatttaga tgtttcaaca ttaattgtag atgatggatc aactgtcgat tcggatatta
     2521 ataaataata tttaaaggga gttaaatcaa taaatagaat atatttactt cgctctacga
     2581 gaaacctagg acatcagcgc gcagtatcaa ttggattagc ttatattgca aatcacttta
     2641 aatcagaagc tgtaatagtt atggactcag atggggaaga taaaccagag tatattaaag
     2701 atcttataaa tacaatgaaa aataatccta acaagataat atttgcagat agaaccaaac
     2761 gttctgagag tttgatgttt cgtctttttt attttcttta caaactattg tttaggacac
     2821 ttactggatc aagaatatct ttcggcaatt tttgtttgat accaaacgat cttctggggc
     2881 gaatagtcac tatatctgat atctggaatt atttttcatc tggcgttatt cactcacagt
     2941 taccttatat gagtataccc acggaacgtg gtagtagaat ttagggtaga ccaaaaatgc
     3001 aatactcttc actaatactt cacggactta gttcaatttc tgtatatatc gatatagttg
     3061 ggataagggt aataatcttt tcaatactat ggatgcttcc tgctttagct ggagttgtaa
     3121 atgccttctc attacgttta tttacaaact tggcatcccc gggatgggct tccactcttt
     3181 ctatagggtt aacaactatt tttatgctct caggtataat ttctttgcta gttgtacttt
     3241 ttatccttag ttatagaaca cagaaacaat ttctaccaaa acgtgattat atagattaca
     3301 tgcttgattt tagtgttcta aagtaatttt aggtcagtag tgtccggtaa aaaattagta
     3361 aaaaggcttg aacgctcctt ataggtgatt aagaataatc accataagcc tattaacaaa
     3421 ggagattcaa gccatgtctc attataacac aatctacaat cagatattac acttaattcc
     3481 cagacatcaa tttgagagta tagttaataa atatgagggg gacctgtatg taaagtattt
     3541 tagttgctgg cagcagctta taacactcct ctttgcacaa ataaggggga aagacagcct
     3601 aagggaaata gagacctccc ttaggacaca gagcaacaaa tggtaccaca tagggcttaa
     3661 agacataaag agatctacac tatcagatgc gaataagcat agggattgga gggtatatga
     3721 ggagttattc tacaggatac ttggcaggtg caaggatgta acacccaagc ataaatttag
     3781 attcaataat cccctataca ctctagatgc aacagtagtg gagttatgtt taagtcttta
     3841 tccatgggca aggttcagga agaggaaggg agcacttaag atacacaacc tttatgacca
     3901 tagcggttgt atacctacat ttatgactgt gacagatgcc agacagcacg atgttaaggt
     3961 ggcaaagaag ctagatttac cccttatggc ggacagcata gtatcagttg atagggctta
     4021 tatagactat aagtggttga attcactgaa tacttcaggt gtttattttg tcactagggc
     4081 aaagagcaat ataaattatg agattatcgg acagcatgag gtgaacaggg aaaagggtct
     4141 actatttgat tatacaatta ggcttagggg gtattatcag agcagatttt atcctgagaa
     4201 attaagactg gttggttttt atgacgctgg aactaacaaa agacttgtgt ttcttaccaa
     4261 taatttctta ctttcagctt atacaataac acagatattc aaggcaaggt ggaagattga
     4321 agagtttttc aggtggatta aacagaacct caagataaaa tcctttcttg gtacttctaa
     4381 gaatgctgtt ctaactcaga tatggattgc catgtgttat tatctgttgc tttcttatat
     4441 taagtaccgg acaaagtact cttatacctt gagtgaattt gcgatcatgc taaaagagac
     4501 tgtacttgag aggattagtc ttattgacct gctaagttgt actcattaca attgctataa
     4561 agccagagag ccagatttgc aattgatact gttttaaccg gacagtactg attttaggta
     4621 tcaaaacttg tgaagaccag ttaatttcta attatatttc ctcatgtaac gctttatgaa
     4681 gacaaacgat taatcaagca agtaaaaagt agcaaagttg attaagggac tgcatccctt
     4741 tattaaccaa cattctttag tcgccaattt gagcaatatt ataatgtatt taatttaacg
     4801 ataacttgaa actatactat tatcaggtaa taacactaaa ggcgatgaaa gtcataactg
     4861 ttccaattgc aatattatta tatatacgaa ttatagtcaa cgataaacat tatacttcgt
     4921 gagatggtta aacttgaaag aataggtatt ataggcggtg gacaactagc ccgtatgaca
     4981 gcttacgctg cttatagaat gggtttagaa atagcaatcc ttgacccgga gccagatagc
     5041 ccggccggac aaatcgcaca tataaagctg gtcggctctt tgaacgatgt tcccatgttg
     5101 gaagaactcg cacgcgtatc taatttaata acacttgaaa acgagttcgt tgacgcacct
     5161 ctacttgaac atcttgagtc tatagggaca tgtgtgttcc cgacagcacg cacagtcggt
     5221 ttaatacagg ataaactctt gcaaaaacaa atcctcgaaa caaatggaat cggtgttcct
     5281 catttcatgg gagtcacaaa ctacgaagac atttatatag caggtgaaag attttgctgg
     5341 ccgcttttac ttaaaactag acgtaacggt tacgatggtc gaggcaatgt attaattcga
     5401 gaaccaaatg atacgttatt agcatggaag acccttgaag gaagaaaatc ctccttgatg
     5461 gtcgagtcgt ttgtgccgtt tatcaaagag cttgcagtaa tggttgcacg aagtagaaat
     5521 agagagattc taacataccc agtagtagaa accatccaga aggatcacat ttgtcatatt
     5581 gtaaaggttc cagcagacgt gggcgaagat gtctctaaaa tggttgcaac tattgccagg
     5641 caagcaattg aagctgtaga tggtgtagga atatttggta ttgagatgtt cattctcgaa
     5701 gacaaaagcg ttcttattaa cgaactcgca ccacgtcccc ataattcggg acactacacc
     5761 attgacgcct gcattacatc ccagtatgaa aaccacctaa gagcgattct ggggctttcg
     5821 cttggccccc caacaatgat tcaaccatct gctgttatgg tgaatctctt aggaaatcgt
     5881 gaaggtatag cccaagtttc tgggatggag aatgcattaa gtatccctgg agtaaacctt
     5941 cacatctatg gaaagaggct aacccatctt ggtcgcaaga tgggacatgt aactgcatta
     6001 ggtcaaaata tcgaggaagc acttgagaaa gccacaagag cggcaagttt tttatcattc
     6061 tgagcgggga tagtaggggc acagcatgct gtgcccctac cgcaaatccg ggtacccacc
     6121 agtcttgctg gtgggcggca tgaaaccata tgctgagata aaatatgagt ggagggttaa
     6181 gcataagtga atgccttaat tggaattgtt atgggaagtg actcagattt gccaacgatg
     6241 aaagcggctg ctgaggtttg tgaagaattt ggcataactt atgaaatacg tatcctatct
     6301 gcacatcgta caccattaga tatggccagt tatgctcaag aagcacataa acgcggactc
     6361 cgtgtgatta ttgcaggcgc tggaggttca gcccatcttc caggtatgat tgcagctcat
     6421 actccgcttc cagttatcgg tgtacccata cgtaccgaag ccttacaggg tttagattca
     6481 cttatgtcta ttgtgcaaat gccgcctggg gtaccggttg caacagtggc aattggtggg
     6541 gcaaaaaatg ccgccttgct tgcaatacaa attcttgcta catccgataa atcaattctt
     6601 caacgtgtaa taagttataa agaaaaactg gctgatacat cacgtgaaaa aaacaagcag
     6661 ctaaagacta gcaaattaag agagcgcaaa aagaagtgaa taaacaagat tcttttacaa
     6721 gcatcgaaaa tgaattgaga taagagctaa ggaaataaag ctcccagcaa caagtttcag
     6781 ggtatcagcg tttaaacgaa caactccttc ggctgccgcg agctgagcaa agcatactgt
     6841 ttgtcattgc gaacacattc gcggagactg tactgagaaa atcgaagtgc ccagtgtaaa
     6901 ctccgcgaag caatctagca cgaagtgtca ttccgaaccc tgtcctgagc ctagtcgaag
     6961 gagcggtgag gaatctagat ctctcacttg cgttcgagat gacacctatt gtcagattcg
     7021 tcgttgcttt tt
//
LOCUS       DATDRL010000200        10121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_822726, whole genome shotgun sequence.
ACCESSION   DATDRL010000200 DATDRL010000000
VERSION     DATDRL010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822726"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VI935_06905"
     CDS             <1..1035
                     /locus_tag="VI935_06905"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE25365.1"
                     /translation="RELGISSGQHVSTFNIGSVYRDMGDYQACLEYYQECWRIIKARG
                     YSSLSGVIAAASGLADIYLLLGNYQESLRYYSEALDIIRGLGDRREEGNILSYMAAIY
                     GILADYQQSVNHYKEALNIYRELGDKASEGRVLTLIGNIYRQNLHDYNRAISFYKEGL
                     EIKKEIEDISEIRSLSNSLGIACWNLGHYEEALAYYQDALEICKNTGNMLGEGITLSG
                     MGVVYLSLRNYEEALKCNIEALNILKATGDQKVEGYILNSIGNVYYEMGAYQMAWKCY
                     QESLRIRKELGDKKGEAWVLHNLGRVYRSLENYDEAKKHYEEALSLAGEVGEEELMAN
                     SKNALSEIKE"
     gene            1142..1405
                     /locus_tag="VI935_06910"
     CDS             1142..1405
                     /locus_tag="VI935_06910"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HLE25366.1"
                     /translation="MPKYLITRTVPPLKEEELAAAGKRSITVCEEMGIRWIKSYYSAK
                     DGKFYCEYEAPSAEAIYEHGRRAQLPVDSVSLILGELDPSMFK"
     gene            2095..3297
                     /locus_tag="VI935_06915"
     CDS             2095..3297
                     /locus_tag="VI935_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLE25367.1"
                     /translation="MVDFELTEAQKNIKEFLHWFAKEKMRSISLQADKEGNVPREFLM
                     EVMELGISSGDVFFGEAEAGEALKDRQGRSQKTRISMIAAEELAWGDPAFIISFPGPG
                     LGAPPIKMLGTPEQKKRFFGIFSDKSEPRWGAYALTEPGAGSDAAAISTTAIKDVDHY
                     ILNGRKCFITNGARASWVVVFATVDPKLGREGHRIFVVEKGTPGFYTGRIEEKMGLRA
                     SETAELIFEECRVPMENLLGGEEYYEGKVKEGFKTAMKTFDATRPIVGAMAVGIARAA
                     FEIVKDWVKENNLLASSLPRFKGLRDNLNDMEREIDAARLLCWRAAWMIDEELPNSKE
                     AAMAKAYSAQVVACVTSKAIEIMGVPGCLRENLLEKLYRDQKVFDIFEGTGQIQRIII
                     SRRIYQSM"
     gene            3441..6224
                     /gene="uvrA"
                     /locus_tag="VI935_06920"
     CDS             3441..6224
                     /gene="uvrA"
                     /locus_tag="VI935_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HLE25368.1"
                     /translation="MKGEITVLGARENNLKNINVSIPWHKFTVITGLSGSGKSSLALD
                     TIYAEGLRRYVECLSTYARQFLERVDRPDMDDITGLPPVIAIESRNQVKSSRSTVGTT
                     TEIYDYLRLLFAKIGRIFCPNCKKEVKHNSPENVAKELLEKNKGKSAIITFPLKESST
                     SQELMSKGFIRVIRNGNVLEVEDILELSEGSEVIVDKVFISNDSFSRIIDSLETAFSE
                     STYINVHILDGNILKYSKELECPYCRKKFDEPTPLLFSFNSPRGACSSCRGFGNILEI
                     DPDLVVPNQDKSLTQGAIEPLTKPSRKYEMKQLLEFARRRGIDINTPFKNLSQKAKEL
                     IFEGDGGSTLLTTGRFPGIKGYFRFLEEKNYKLHVRVFLSKYRSAFICGACNGSRLRP
                     ESLWVKVCGKTIHEFVEMPIKDLKIFFESLSLTDYEGKIAKEILKQIRSRIDFLSKVG
                     LQYITLSRLTKTLSGGEAQRVDLACQLGASLTDTLYVLDEPSIGLHPKDIDRLVSIIK
                     ELRDRNNTVVIVEHDFNMIGSADYIIELGPLAGEKGGDVVYQGSVENFLNNSNQSLTK
                     RYLTHQEEIPFPIKRRKSKGRYLTVVGARENNLKNINVSFPLGTFICVTGVSGSGKSS
                     LMHDVLYSSLARRFRSEAEKVGKFKEISGTSNLSDVLMLDQTPIGKSSRSNPVTYIKA
                     YDEIRRVMSSTWDSRSKGFQASHFSFNVPGGRCDTCQGEGRQKIEMHFLADVFITCEE
                     CKGKRFKKEVLEVRYKGSNIDEVLNLTVDEAINFFLEFPPLLRRFKILQDVGLGYLKL
                     GQPAPTLSGGEAQRIKIARELGRKASPELSRRNGKDILYILDEPTVGLHSEDIKKLLR
                     VLNKLVDAGNTVIIIEHNLDVIKSADFIIDLGPEGGDEGGYIVAQGTPEEISRVEGSY
                     TGMYLSKVLSNIF"
     gene            6613..6942
                     /locus_tag="VI935_06925"
     CDS             6613..6942
                     /locus_tag="VI935_06925"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HLE25369.1"
                     /translation="MGMVVSRFEVYLVRLDPTEGHEIQKARPCIIISPDEMNHHINTV
                     VIAPMTTKGNPYPTRVPVRFKRKVGQIVLDQIRTVDKSRLVKRLGKIDEATADKVLAV
                     LTEMFAP"
     gene            complement(7065..7925)
                     /locus_tag="VI935_06930"
     CDS             complement(7065..7925)
                     /locus_tag="VI935_06930"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HLE25370.1"
                     /translation="MDYLKIAENAAREAGLLLREKFGKVKEVEYKTKNSLVTEVDRLS
                     ENLIVKVIRENFPSHDIFAEEAGRISNKSTHVWIIDPLDGTTNYAHTYPFFAISIALE
                     VEGVIRLGVVYDPIRDEFFSAELGQGAYLNGNSLKVSKTGKIEESLLCTGFTHENEWM
                     VDENLTHFENFIRRAQAVRRDGSAALDLCYVACGRYDGFWELGLHSWDTAAGSLIAKE
                     AGGRITDFSGGSGVSPISPTGSGTGIKREGYKGTAQEFDIYGEETLATNGLIHDEMIR
                     VLSLGREPHI"
     gene            complement(7937..8365)
                     /locus_tag="VI935_06935"
     CDS             complement(7937..8365)
                     /locus_tag="VI935_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLE25371.1"
                     /translation="MDKKVSEIFKLSKECVFVLNVRRDEESLEFVYPPKMKGNLLPIN
                     TKSIAGSAVLNRRPYISNNVRLEKSFIIFDWIMDRGGTPIQKMISYPVLLTEKVISVI
                     QITRRGDSLSEAGPDFDKSDLEKLSSILNSFLALQVVSSA"
     gene            8568..9011
                     /locus_tag="VI935_06940"
     CDS             8568..9011
                     /locus_tag="VI935_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HLE25372.1"
                     /translation="MKIAVGSDERTHLTDFVIEELKKRGIELELHGPLSGENIQWADV
                     AQNVAERVANGKCDQGILFCWTGTGVSMAANKVPGIRAALCADAETARGARKWNDANV
                     LAMSLRLTSPIIAKEIIDAWFSASLEKEEASNVEKVKAIEEKYNR"
     gene            complement(9155..9925)
                     /locus_tag="VI935_06945"
     CDS             complement(9155..9925)
                     /locus_tag="VI935_06945"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HLE25373.1"
                     /translation="MKKFMYFIVLAVIAFTYSQPSYSQSSEELKAIKEDLKALKEGQA
                     NMQKELQEIKTLLKSRPAAAPSEPQNVVLNIDKAPFKGNKNAKLTLIEFSDYQCPFCG
                     RHVTQTLPQLDTDYIKTGKVKYVFLDFPLDFHKNAFKAAEAADCAGEQGKFWQMHDLL
                     FANQKALELSDLTNYAKDLKLDTSKFQKCLDSGKYASQIKKEIELGQKSGVTGTPSFF
                     LGVTNPKDPNVKAVKVIKGAQPYANFKDAIDTTLSSQK"
BASE COUNT         3157 a         1942 c         2260 g         2762 t
ORIGIN      
        1 agagaattag ggatttcatc cggtcagcat gtttcgacat ttaatatcgg tagtgtttat
       61 cgggatatgg gcgattatca agcctgcttg gaatattatc aagaatgttg gagaataatt
      121 aaggctaggg gatacagtag cctttccggg gtgattgctg cggcaagcgg tcttgcagat
      181 atatatctac tcttaggaaa ctatcaagag tcccttagat actatagtga agcattggat
      241 ataatccgcg gattagggga tagaagggag gaggggaata tcctaagtta catggcagca
      301 atttatggta tcttagcaga ttatcaacaa tcagttaatc attataaaga ggcgttaaat
      361 atttatagag aattaggaga caaggcatca gaaggaaggg ttcttactct tatagggaat
      421 atataccgtc agaatctaca tgattataat agggccattt cgttttataa agaaggcttg
      481 gaaattaaga aagagataga agacatttct gaaattagaa gcttatcaaa tagcctagga
      541 atagcttgct ggaacttagg gcattatgaa gaagcccttg catactatca agacgcttta
      601 gaaatctgta agaatactgg aaatatgtta ggcgaaggaa taacactaag tggcatgggg
      661 gttgtttatc ttagtttacg taactacgaa gaggctttaa aatgtaacat agaagcccta
      721 aacatattaa aggcgacagg agaccaaaag gtagagggat atattttaaa ttctattgga
      781 aacgtgtatt atgaaatggg tgcttaccaa atggcttgga aatgctatca agagtctttg
      841 agaataagaa aagaacttgg agataaaaaa ggagaggcct gggttcttca taaccttgga
      901 agggtttatc ggagcttaga aaattatgac gaagctaaga agcattacga agaagctctt
      961 tctttggcag gagaggtagg agaagaagag ttaatggcaa atagtaaaaa tgccttatcc
     1021 gagatcaaag aatgaatctt ttttaaaatg cgggacagga atgtcccgcc tatcttaaat
     1081 agaatttaac acatgcgata gacgggattt tctaatcccg tctaacaagg aggtaaaaca
     1141 gatgccaaaa tatttaatta cacggacagt gcctccgtta aaggaggaag aactcgccgc
     1201 tgcaggaaaa cgctctataa ctgtctgcga agagatggga ataaggtgga taaagagcta
     1261 ctattcagca aaagacggaa agttctactg tgagtatgaa gcacctagcg ctgaagcaat
     1321 ctatgaacat ggaagacgtg cacaacttcc ggtagattct gtctctctta ttttaggcga
     1381 acttgaccct agtatgttta agtagggcag attttaaatc tgcccctaca tcaaatgaat
     1441 ggaaagaaag ggggtggtac agatgccgaa atttttaact gtgcggtcag tacctccctt
     1501 aacggatgaa caaatcgctg ctgcatccaa acgtgctata tctttagggg aacagatagg
     1561 tgtaaggtgg gtatagagct accattcagc aatagacgga aagctctatt gtgaatggga
     1621 agcaccaaac attgaagcta cctacaaaca cgcaaagctt ctacaaaggc ctgttgattc
     1681 agtatcactt gtatcagggg aatttgaccc cagtatgttt aagtagaaat cggttaaaaa
     1741 gcatttcata acttcactcc gattagttat accattttca ttatgaatta aggtattcag
     1801 tgtaaagcgg tcacgaacaa gcctgccatg agtcaatcga agtgactcgt agcccattgg
     1861 taggttggta aatttcatct tgctaatacc tcagataggc agggttttct aaccctgcca
     1921 gccgagacaa gaatgtcacg gctatcgtcg tcttatgcaa tgggcccaca agtcccctca
     1981 gctaagttcg ggacaggctt gctggtggac ttatgttatg aatgctatca atttaaaaat
     2041 tcaataggaa aatggtgtta caataatgat gatcccccag ggaggtttca ctaattggtt
     2101 gactttgaac tcaccgaagc tcaaaagaac ataaaagagt tcctacactg gtttgcaaaa
     2161 gagaagatga ggtctatatc ccttcaagca gataaggagg gtaatgtacc gagagaattt
     2221 cttatggagg tgatggagct aggaatcagt tcaggagacg ttttttttgg ggaggctgag
     2281 gcgggagaag ctcttaaaga cagacaagga aggtctcaaa aaacaagaat atctatgatt
     2341 gctgctgaag aacttgcatg gggagaccct gcatttatta tttcatttcc aggtcctggt
     2401 cttggggctc ctccaataaa gatgcttgga acacctgagc agaaaaagag attctttgga
     2461 atatttagtg ataaatcaga gcctagatgg ggtgcctatg cgcttacaga gcctggtgcc
     2521 ggttcagacg ctgcggcaat aagcacaact gctattaaag atgtcgatca ttacatatta
     2581 aatggaagaa agtgctttat tacaaacggt gcaagagcaa gctgggttgt agtctttgca
     2641 actgttgatc ctaagctagg aagagaaggc caccgaatat tcgtggttga aaagggaaca
     2701 ccgggatttt atacggggag aattgaagag aaaatggggc tacgagcatc agaaaccgct
     2761 gagcttatat ttgaagagtg cagagtccca atggagaacc tgcttggtgg agaagaatac
     2821 tacgaaggga aggtaaaaga gggtttcaag acagctatga aaacgtttga tgcgacacgc
     2881 ccgattgttg gagctatggc tgttgggatt gcacgcgcag cctttgaaat agtaaaagac
     2941 tgggtaaagg aaaacaatct ccttgctagt tcccttccta gattcaaagg tcttagagat
     3001 aaccttaatg acatggaaag agagatagac gctgctagac ttctatgttg gcgcgcagcg
     3061 tggatgattg atgaagaact accaaattcg aaggaagcgg caatggcaaa agcctattca
     3121 gctcaagtag tagcttgcgt tacatcaaaa gccatcgaga ttatgggggt tcccggatgc
     3181 ctaagagaaa accttttgga aaaactctac agagaccaaa aggtgtttga catatttgag
     3241 ggcacaggtc aaatacagag aatcataatc tcacgtagga tttaccagtc aatgtgagat
     3301 cttagccaca gagttcacgg agaaaagaat tatttgcgta ggggcacgtt gcaacgtgcc
     3361 cctactttct gatcaatgtt ctcagtgggc tcggtgtcta aagtaagaaa agcaatagta
     3421 cggtataatt tcttaatatt atgaaaggcg agatcactgt attaggggcc agggaaaata
     3481 atttaaagaa tatcaatgtc tctatacctt ggcataagtt cacagttata actggcctta
     3541 gcggttcagg taaatcttct cttgcgcttg atacgatata tgccgaggga ctcagacgat
     3601 atgttgagtg cctttccact tatgcaaggc agttccttga acgggttgac cgcccagaca
     3661 tggacgatat cacggggctt cctccagtga ttgctattga aagcagaaac caggtaaaaa
     3721 gctcacggtc tacagtagga acaaccactg aaatctatga ttatctaagg cttctttttg
     3781 caaagatcgg aaggatattc tgtcctaatt gtaaaaagga agtaaaacac aactcccctg
     3841 aaaatgttgc aaaagaacta ttagaaaaga ataagggtaa aagcgcaata ataaccttcc
     3901 ctttaaaaga aagctcaacc tctcaggagc taatgtctaa aggttttata agagtaatta
     3961 gaaatggaaa tgttttagaa gttgaagata ttttagaact ctctgaaggc tccgaggtca
     4021 ttgttgataa ggtatttatt agcaatgact cattttcaag aatcattgac tcccttgaga
     4081 ctgctttttc tgaaagcaca tatataaatg tccatatctt agatggcaac atacttaagt
     4141 attcaaaaga gctagagtgt ccatactgcc gaaagaagtt tgacgagcca acgccacttc
     4201 ttttctcttt taatagtcct agaggggcct gttctagctg tcgtggtttt gggaatattc
     4261 tcgaaataga ccctgactta gtggttccta atcaggataa gagcctaact cagggtgcca
     4321 tcgaaccgct cacaaaacca tcgcgtaaat acgagatgaa acaactccta gaattcgcac
     4381 ggagaagggg aatagacatt aacacacctt ttaaaaatct ttcccaaaag gcgaaagagc
     4441 tgatatttga aggcgatggt ggttcgactt tgctcaccac aggcagattt cccggtataa
     4501 aaggatattt tagattttta gaggaaaaga actacaagct tcacgttagg gtttttctca
     4561 gtaaatacag gtcggctttc atatgcggag cttgtaacgg tagcagatta agacccgaat
     4621 cactctgggt aaaggtctgt ggaaaaacga tccatgaatt cgtagagatg ccgattaaag
     4681 atctaaaaat attttttgaa agtttaagcc taactgacta tgaaggaaaa atcgcaaagg
     4741 agattctcaa acaaattagg tctagaatcg attttctatc aaaggtagga ttgcagtaca
     4801 ttacactttc tagacttact aagaccctat caggtggaga agcacagagg gttgacttgg
     4861 catgtcaact cggtgctagc cttactgaca ccctttacgt cttagatgaa ccatcaattg
     4921 gactacaccc caaggacatt gacagactgg tttcgataat taaagaactg agagatagaa
     4981 ataacactgt agtaatcgtt gaacatgatt ttaatatgat aggttcggct gattatataa
     5041 ttgaattagg gccattagcc ggagaaaagg gtggcgatgt cgtataccag ggatcggttg
     5101 agaatttcct gaataactct aaccaatctc ttacaaaacg gtatttaaca catcaagaag
     5161 agatcccgtt ccctataaaa agaagaaaaa gtaaagggag atatttaacc gttgtaggag
     5221 ccagagaaaa caatctcaaa aacattaatg tttcatttcc acttgggact tttatatgcg
     5281 taactggggt ttccggttct ggtaaaagct cgcttatgca cgatgttctt tattcaagtc
     5341 tagctcgaag atttcgaagc gaggcagaga aggtaggtaa gttcaaggaa atctcaggta
     5401 catccaatct ctccgatgta cttatgcttg atcaaacacc cattggaaag agctcaaggt
     5461 caaaccccgt aacctatatt aaggcctatg atgaaattag aagggttatg tcaagcacat
     5521 gggattcacg gtcgaaggga tttcaagcct cccatttctc attcaatgta ccgggaggaa
     5581 gatgtgatac ctgtcaggga gaaggtagac aaaagataga gatgcacttt cttgcggacg
     5641 tatttataac ctgtgaagag tgtaagggaa agagatttaa gaaagaggtg cttgaagtaa
     5701 ggtacaaagg tagcaatata gacgaggtcc ttaaccttac tgttgatgaa gcgatcaact
     5761 tcttcctaga attcccaccc cttcttagaa gatttaagat acttcaggat gtgggacttg
     5821 ggtatttaaa attaggacag cctgcgccta ccctatcagg aggagaagcg cagagaatca
     5881 aaatcgcaag ggagctaggg agaaaggcca gtcctgagct aagtcgaagg aacggtaaag
     5941 atatactcta catcttagat gaaccaacgg taggtcttca ctcagaggat ataaagaagc
     6001 tgctcagggt gttaaataaa ttagttgatg caggaaatac agtcataata attgaacata
     6061 atttagatgt aataaaatcc gcagacttta taattgacct gggcccagag ggcggcgatg
     6121 agggtggata tatagtagct caaggtacac ctgaagaaat ttccagggtt gaaggatcgt
     6181 acaccggaat gtatctaagt aaggttctgt cgaacatctt ctaatacaga taggtactat
     6241 tgtaagttag atgggaccct tataaaaaga ataagaaacc ctcagactcc acttcaagct
     6301 tataccttga cgatggcgaa gtttgatggt aattactatg taattactta atggagaggc
     6361 cgcatcaacg atgaaaacaa acattatccg tatcggcaat tccttgggta tacgtatccc
     6421 caagttgttg ttggagcagt gccgcttgaa aggcacggtg gaactacagg tgcaaaacga
     6481 acatttgatt atcaggtcgg ttgatagacc acggagcggc tgggaggatg cattccgcca
     6541 tatggccctg caaggcgacg atatgcttct aaatcgagac tcctggccta agactcaatg
     6601 ggatgaaact gaatgggaat ggtagtctcc cgttttgagg tgtacctagt acgacttgat
     6661 ccaaccgaag ggcatgaaat acagaaggct cgtccctgta ttattatctc ccctgatgaa
     6721 atgaaccatc acatcaatac cgtcgtcatt gctccaatga ctactaaggg gaatccatat
     6781 ccaacacgag ttcctgtgag attcaaaagg aaagttgggc aaattgtcct agaccagatt
     6841 cgcactgtgg ataaaagccg gctggttaaa cgtttgggca aaatagatga ggcgactgcc
     6901 gataaggtgc ttgcagtact aactgagatg ttcgcacctt agtcactcta tccataatcg
     6961 acctaggccc agatggagga gtcaatcgaa gacataaagc gtgaggttta taagatagat
     7021 aaaatcaaat aaaaaaaggt gttagctgag gtttctaggg ttgttcaaat atgtggttct
     7081 cttcccaggg ataaaaccct aatcatttca tcgtgtatta agccatttgt tgcaagcgtc
     7141 tcttcgccat agatgtcaaa ttcctgtgcg gttcccttat aaccctccct cttgatcccc
     7201 gtccccgatc cagtcgggga gatcggggat accccagacc cccctgaaaa atccgttatc
     7261 cttccccctg cctctttggc aatcaaagag ccagcagcgg tatcccagga atgaagccct
     7321 aactcccaga aaccgtcata ccttccacac gctacatagc aaaggtcaag ggctgcagaa
     7381 ccatcacgcc ttacagcctg cgctcttctt ataaaatttt caaagtgtgt tagattctca
     7441 tctaccatcc attcattctc atgagtaaaa ccagtgcaaa gcaggctttc ctctatcttg
     7501 cctgtctttg agaccttcaa ggaatttcca ttcagataag caccttgacc aagctccgca
     7561 ctgaagaatt catccctaat agggtcgtag accactccta atctaataac cccttccacc
     7621 tctagtgcaa ttgatattgc aaaaaaagga tatgtatgag cgtagttagt tgtcccatcc
     7681 agggggtcaa tgatccaaac atgagtagat ttattactga ttcttcctgc ctcttcggca
     7741 aagatgtcgt gagaaggaaa gttctctcta atgactttta ctatgagatt ttctgataat
     7801 ctgtcaacct cagtaacaag gctgttttta gtcttgtatt caacctcttt tacttttccg
     7861 aatttttctc taaggagcag tcccgcctct cttgcggcgt tctcagcaat ttttaggtag
     7921 tccaaaaccg cttcctctaa gcagagctca caacttgaag agcgaggaaa ctattgagaa
     7981 tagaacttaa cttttcaagg tccgatttat caaaatcagg tccagcctca gaaaggctat
     8041 cacctcttct cgtaatctga atgactgaga taaccttctc tgttaataag actggataac
     8101 taatcatctt ttgaatcgga gtgcctcccc tatccataat ccaatcaaaa ataataaagc
     8161 tcttctctaa acgcacatta tttgaaatgt aaggacgccg gttaagaacc gcgcttcccg
     8221 ctatgctttt ggtgttgata ggaagcaaat ttcctttcat ttttggcggg taaacaaatt
     8281 ccaatgactc ttcgtcacga cgaacattta gaacaaaaac acactctttg gaaagtttaa
     8341 atatctctga aaccttcttg tccaataata agtgatctat aattatttgc ctcctttaac
     8401 ctttatcagt atcaggccgt gagcacatat tttataacaa aaagcccata agtcaataag
     8461 gttttgggta tgactttggt atagtttctt tatcgagtta aggcctcctt gaacccctgt
     8521 tatacctaat ttaaatttat atacatagtc agtagggggt aacctatatg aaaatagccg
     8581 ttggaagcga tgaacgcacg catttaaccg attttgttat cgaagaatta aagaaacgcg
     8641 gtattgagct cgaacttcat ggtccacttt caggtgaaaa tattcaatgg gcagatgttg
     8701 ctcaaaacgt agcagaaagg gttgcaaacg gaaagtgcga tcagggaata ctattctgct
     8761 ggacaggtac aggcgtaagt atggcagcga acaaggtgcc aggaatcagg gctgctttat
     8821 gcgctgatgc agaaactgca agaggagctc gaaagtggaa tgacgcaaat gtattagcaa
     8881 tgagccttag acttacctct ccaattatag caaaagagat aattgatgcg tggttcagtg
     8941 ccagcctcga aaaggaagag gccagtaacg ttgaaaaggt aaaagcgatc gaggagaagt
     9001 ataaccgata aagcttagta atcgccccat ggtgtgtcac accgcagtga gaaaaaaatg
     9061 tagggactga tcaccgtatc agtcctcata tcgatgcccg tcctaggaac tggccctaca
     9121 tcggtggagt ggttcaagga actatcgagt tagactattt ctgcgaagaa agcgtcgtat
     9181 ctatagcatc cttaaaatta gcgtagggct gtgcaccctt tatcaccttt acagccttca
     9241 catttggatc cttcggattt gttacaccta agaaaaagct tggcgtacca gtaactcctg
     9301 acttttgacc tagttctatc tccttcttga tctgactcgc atactttccg ctatccaggc
     9361 acttctggaa tttcgacgtg tccagcttaa ggtcttttgc ataattagta aggtcactaa
     9421 gttccaatgc cttttgattt gcaaaaagga gatcatgcat ttgccagaac ttcccttgct
     9481 cccctgcgca gtcagctgcc tcagcggctt taaatgcgtt cttatggaag tctaaaggga
     9541 aatcgagaaa aacgtatttt actttgccag tctttatata atctgtatct agctgtggga
     9601 gtgtttgagt aacgtgcctt ccacaaaatg ggcactggta atcagagaac tcgattaagg
     9661 ttagttttgc gtttttattc cccttaaacg gagccttatc aatatttaat acaacattct
     9721 gtggctccga aggagcggct gctggccttg atttgagaag tgtttttatc tcctgaagtt
     9781 ccttttgcat attcgcctgc ccttctttga gcgcctttaa atcctccttt attgccttta
     9841 attcttcact agattgtgaa tagctgggtt gactatatgt gaatgcaatc actgccagga
     9901 caataaaata catgaatttt ttcatctcga ttttctcctt atataaatat atagatttag
     9961 gtaagaataa tatgtgtaat ccacggttct gtcaaatggt taaaggaaaa tggggtcagg
    10021 tctttaattg tgcattattt ctgatttaca attctactca ctgtcgtgta atgcaacccg
    10081 gctacccttg caatttctac aagcgtgtac ccatagctta a
//
LOCUS       DATDRL010000201         3208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1338347, whole genome shotgun sequence.
ACCESSION   DATDRL010000201 DATDRL010000000
VERSION     DATDRL010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338347"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VI935_06950"
     CDS             <1..213
                     /locus_tag="VI935_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLE25374.1"
                     /translation="PPKATFYVWFEVPDHITSKDFTGKLLTESGIVVTPGVGFGKYGE
                     GYIRVSITIAKERLIEAIERLRKSRF"
     gene            252..1799
                     /locus_tag="VI935_06955"
     CDS             252..1799
                     /locus_tag="VI935_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HLE25375.1"
                     /translation="MNLESLVVFTKGFLTAIYLMTFGLLCIFGLHRYYLAHLFTKYKN
                     RIPKPKEKLKKLPVVTVQLPIYNEMYVVERLISSVCNIDYPKEVLEVQVLDDSTDQTQ
                     EISRKIVDSFRAQGFHIHYLHRENRKGFKAGALEEGLKSAKGELIAVFDADFIPPKDF
                     LRKTVDFFSDPKIGMVQVRWGHVNLDYSTLTRAQSILLDGHFVIEQASRYFGGRFFNF
                     NGTAGVLRRECIESAGGWQHDTLTEDLDLSYRAQLKGWRFVYLKDFVANAELPVDMNA
                     FKLQQHRWTKGAIQTAKKLLPQILKSKDLPIQIKIEAAFHLLGNFSYVLLLLLLFLML
                     PISYLWRSIGWEKVVLLNLFAISTGTISVIRFYIITLKEVYGKNWIRYAKYIPLSIAL
                     GTGIALNNSRAVFEALFSKNSEFKRTPKFAVTTSKDDWRTRSYTTSKEITTLIELAIG
                     LLFTFQTFYAIFMGYLGWIPFLIIIQVGFLYTSTLSIFHGWRRLPMSINSLELAPKPT
                     LTKENNL"
     gene            complement(1978..3150)
                     /locus_tag="VI935_06960"
     CDS             complement(1978..3150)
                     /locus_tag="VI935_06960"
                     /inference="COORDINATES: protein motif:HMM:NF040309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HLE25376.1"
                     /translation="MSKIQYIFQAGSLYREFRERRMRFGVNLPQLTQNGVSIHKVDFV
                     SNNYSCLLREDQDVADNIQFPYMLFLPEGNKQFDEVIIILNGLNESEYRKFFPWAASF
                     AASGIPTIIFPITFLINRRPRSWFIPEEVGKKLSIRRNLEGNSTCTSYNVILSERLYE
                     HPERFFLAGLQTHNDLIDLVNALYYGEYSFWREDRTFSPFTKGTRAHFLGYSLGGYLA
                     LILFLGVGDNPILSQSKLIILCSGAAINNDVTDLNSNPISPLILDRNASEKLIEFYKQ
                     GESFPSMGTVEALMFKAVFLSDQSILGPNLERLRKRIRIIGSGNDKVIPLKGIEKNLG
                     WVDENLKLGIHEYPFNVQSHDQPNLEREMSRSYDIAKEFQEGFKRFVDSTITAVCD"
BASE COUNT          979 a          636 c          593 g         1000 t
ORIGIN      
        1 cctccaaagg ctacctttta tgtctggttt gaggttccag atcacattac ttcaaaggac
       61 tttacaggaa aattacttac ggagagtgga atagtggtta cacccggcgt tggattcgga
      121 aaatatggag aaggatacat aagggtatca attacgattg caaaagaaag acttatagaa
      181 gcgatagaga gacttagaaa gtcgaggttt taagttaatt atcttgaaaa ctaactattg
      241 aaaagatcca tatgaaccta gagagcttag ttgtatttac caaaggattt cttacagcta
      301 tctatttaat gacattcggg cttctctgta tcttcggtct tcatagatac tatcttgcgc
      361 atctttttac caagtataag aatcgaatcc ctaagcccaa ggagaagcta aaaaaactgc
      421 cagttgtcac cgttcagctt ccgatttata acgagatgta tgtcgtagaa aggctgatct
      481 catcagtttg taatattgac tatccaaaag aagtcctaga ggtccaggtg cttgacgatt
      541 caactgatca aactcaagag atctcccgaa aaatcgttga ttcgtttaga gctcaaggct
      601 ttcacataca ctacctccat agggaaaaca gaaaggggtt caaagctggc gcgttggaag
      661 aagggctaaa atctgctaaa ggagaactga ttgcagtgtt cgatgcagat tttattccgc
      721 caaaagattt tctaagaaaa acagtggact tcttctcgga tcctaaaatt ggcatggttc
      781 aggtaaggtg ggggcacgta aacctagact actctaccct tacaagggct caatctatac
      841 tacttgatgg gcactttgtc attgagcaag catctaggta ttttggaggt agatttttta
      901 acttcaacgg cactgcggga gttttaagaa gggagtgtat agaatcagcg gggggatggc
      961 agcacgacac gcttacagag gatcttgatc ttagttacag agcgcagctt aaaggatgga
     1021 ggttcgtata ccttaaggat tttgtagcca atgctgagct tcctgttgat atgaatgcat
     1081 ttaagttaca acaacacagg tggacaaagg gagcgattca aacagctaag aaactccttc
     1141 ctcaaatact caaatctaag gatcttccta tacagataaa aatcgaagct gcatttcacc
     1201 ttttaggtaa cttttcatac gtgcttttat tacttctttt attcttaatg cttccaatat
     1261 catatctatg gcgttccata ggatgggaga aagtagtgtt actcaatcta tttgcgatat
     1321 caaccggtac gataagtgtg ataaggttct atataattac tctcaaagag gtttatggaa
     1381 agaactggat acgttatgca aaatatattc cactctcaat cgcccttgga actgggatag
     1441 ctcttaataa ctcgagggct gtatttgaag ccttgttctc aaaaaactct gaatttaaaa
     1501 gaactccaaa atttgctgta accactagta aagacgactg gcgtacaaga agctatacca
     1561 catcaaaaga gataaccacg ttaatagaac ttgcaatagg acttcttttc acgttccaaa
     1621 ccttttacgc aatatttatg ggttaccttg gttggattcc ctttcttatc attattcaag
     1681 tgggctttct ttatacctca acactctcta ttttccatgg ttggcggcgg cttcccatgt
     1741 caataaattc actagaatta gctccaaagc ccacccttac aaaagagaat aatctttaga
     1801 tatacacgtc agctatcata aaatttcaaa caaaatttta ggaggtcaat tttcttgatt
     1861 aagtaactag aatttgaaca gcaattagca ttttcaaatc ttgtgttctt ttacaaaaat
     1921 tcaattgcat ctgtttctgt atactttaaa ttgttatttg ttactgaatc tcccttttca
     1981 atcacaaact gctgtaattg tactatctac aaacctttta aatccttcct gaaactcttt
     2041 tgctatgtca tagcttcttg acatctccct ttctagatta ggctgatcgt ggctttgaac
     2101 gttgaaggga tattcatgga tgccaagttt taaattctca tctacccagc ctagattctt
     2161 ttctattcct ttaagcggta taaccttatc atttccgctt cctattatcc ttattctctt
     2221 tcttaatctc tctagattag gaccaagtat ggattgatcg cttaaaaaaa ccgccttgaa
     2281 catcaaggcc tcaactgtcc ccatagaagg gaaactttca ccttgtttat aaaactcaat
     2341 tagcttttca ctagcatttc tatcaagaat aaggggacta atcgggtttg agtttaaatc
     2401 cgtgacatca ttattgattg ctgctcccga acataagatt attagtttac tttgagataa
     2461 aatggggtta tcccctactc ccaaaaaaag aatcaaggca aggtagcctc ctagtgagta
     2521 acctagaaag tgcgcccttg tgcctttggt aaagggagag aacgtcctat cttctcgcca
     2581 aaaactatat tctccgtaat ataatgcatt gaccaaatca attaggtcat tatgagtttg
     2641 caaaccagca agaaaaaacc tttcgggatg ctcataaagc ctttcactca agataacgtt
     2701 ataagaggta caggtgctgt taccttccag atttcttcta attgatagct tcttcccaac
     2761 ctcctcaggt ataaaccaac tccttgggcg cctatttatc agaaaggtta tagggaaaat
     2821 aattgtagga atacccgatg ccgcaaaact tgctgcccag ggaaagaatt ttctatattc
     2881 gctttcgtta agcccgttga gaatgattat tacttcgtca aactgtttgt taccctctgg
     2941 aaggaaaagc atgtacggga actggatgtt atctgctaca tcttgatcct ctctaagaag
     3001 gcaactgtag ttatttgata caaagtctac cttgtgtatt gaaactccat tttgggttaa
     3061 ctgaggcaga ttcaccccaa atctcatcct tctttcccga aattctcgat atagagatcc
     3121 ggcttgaaaa atatattgga ttttagacat ttttctctcc aatcaagagt agtccgtaat
     3181 aaacttttta gttagggtta gaaaaccc
//
LOCUS       DATDRL010000202         3519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1358865, whole genome shotgun sequence.
ACCESSION   DATDRL010000202 DATDRL010000000
VERSION     DATDRL010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358865"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1900)
                     /gene="pbpC"
                     /locus_tag="VI935_06965"
     CDS             complement(<1..1900)
                     /gene="pbpC"
                     /locus_tag="VI935_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HLE25377.1"
                     /translation="MILTFSYLFTTYANAIPTFDEVKNSYKKSDAVLLDRHGEIIHEL
                     RVDEKGRRLEWISLKDISQSLIKAVIHSEDKRFYDHHGVDWRAIEVAIFESLVSKNNR
                     GASTITMQLASTLDKDLKPKDSKRTLRQKWNQIRAAWEIEKTWSKAEILEAYLNLVTF
                     RGELQGVSSASRGLFDKEPSGLNESESTIIASLIRSPNAQVSDVIKRACILASSIKSQ
                     TQCENIKSLAKTLSGTYSIRPRMSLAPHVARELLQKEGTRAVSTLDADIQRFSTEVLK
                     HQLSSLKNQNVHDGAVIVVENKTGDILAYVGNSGLSSSARYVDGVRARRQAGSTLKPF
                     LYQLAIEKRLVTAASILDDSPLDISTPVGLYIPQNYDNEFKGLVSVRSALSASLNVPA
                     VRTLMLIGTEPFVERLKELGFENLKESGDYYGFSLALGSADVSLYELVNGYRTLANNG
                     IWSELSLSFDKNTSKKQVLEKDGTFIISDILSDRESRSATFGFENPLSTRYWTAVKTG
                     TSKDMRDNWCIGYSQKYTVGVWVGNFTGEPMWNVTGLTGAAPIWLDLMNYLHRSYPSI
                     PPQPPLGVVTKRIEFDHDIEPEKDEWFIEGTQPSSIELNTIHVNPHIIYPSERTIIAL
                     DPDIPERHH"
     gene            complement(2118..3416)
                     /locus_tag="VI935_06970"
     CDS             complement(2118..3416)
                     /locus_tag="VI935_06970"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25378.1"
                     /translation="MAELPSYLDSDGLVKYFPKMDLGSDVLTSYILSISSEAKYEIPS
                     ELKNRMIDGLKGFIEGRVIRYSSLPTADLSIRKMAVLEALSRNGESDPNLLGSISIEP
                     NLWPTSAVIDWMNVLLRIKDIPDRDKKLKESEQIIRSRLNLRGTTMSFSTESTDYLWW
                     LMISTDENAARGLLTLISFENWKDDIPRMARGVIGRQKRGHWDTTVANAWGVLAMGRF
                     SEKFESIPVTGSTSAELYGTTKTVNWKESPKGKSGMFSWPDTKEELSIVHQGSGKPWA
                     TIESLAAIPLKEPFSSGYKIKKTLIPVEQKIAGKWSTGDVMRIRLELEAQADMTWVVV
                     NDPIPAGSAILGTGLSRDSEVLTQGEESKGWVWPAFEERSFEAFRTYYEYVPKGKWTA
                     EYTLRLNNEGVLGLPPTRVEALYSPEMLGEMPNKEIRVEP"
BASE COUNT          919 a          799 c          658 g         1143 t
ORIGIN      
        1 ggtggtgtct ttcgggaata tccggatcta aagcgattat agttctctct gacggataga
       61 taatatgggg atttacgtgg attgtattta attcaattga acttggttgt gtgccctcta
      121 taaaccactc atctttttca ggctctatat catgatcaaa ttcaatcctc tttgttacca
      181 ctcctaaagg tggttgagga ggaatactgg gatagctgcg atgaagatag ttcataagat
      241 cgagccagat aggcgctgct cctgttaagc ctgtaacatt ccacattggt tcacctgtga
      301 aatttccaac ccagacacca accgtatatt tttgcgaata tcctatacac cagttatccc
      361 tcatgtcctt actcgttcct gtttttaccg ccgtccagta tcgggtggat aatgggtttt
      421 caaatccaaa agttgcgctt ctagattccc tatctgaaag aatatctgag ataataaatg
      481 taccgtcttt ttcgagtacc tgttttttgc ttgtattctt atcgaaactg agactcagtt
      541 cactccatat tccattattt gctaatgttc ggtatccatt tacaagttca tataggctta
      601 catcggcaga accaagggca agtgagaaac catagtaatc tcctgattct ttaaggtttt
      661 caaatccaag ttctttaagt ctctcaacaa aaggttctgt tccaattagc atcaaggttc
      721 ttacagcagg aacgttaagg gatgctgaaa gagctgaccg aacactgaca agacccttaa
      781 attcattatc gtagttttgt ggtatataaa gcccaaccgg cgtcgaaata tcgagtggag
      841 agtcatcaag gatagaggct gcagtaacta gtcgtttctc aattgcaagt tgatataaaa
      901 atggcttgag tgtcgagcct gcctgtctcc tagctctaac tccatctaca tatctcgcac
      961 ttgaagatag cccgctattt ccaacatatg caaggatatc cccagtctta ttttctacaa
     1021 cgatcactgc tccatcatga acattttgat tctttaatga actgagttga tgttttaaaa
     1081 cttcggttga aaatcgctga atatctgcat caagcgttga aacggctctt gttccttctt
     1141 tttgtaaaag ctctcgggca acgtgaggcg cgagtgacat acggggtctt atggaatagg
     1201 tacccgaaag cgttttggca agactcttta tattttcgca ttgagtttgc gacttaatag
     1261 aacttgcaag aatacatgcc ctttttatta catcactaac ttgagcattt ggtgaacgga
     1321 ttaaagatgc tattatggtg gattctgact catttaatcc acttggctct ttatcaaaaa
     1381 gccctcttga tgccgaggat acaccttgaa gttctcccct aaacgttact aaatttagat
     1441 atgcctcaag tatctcggcc tttgaccatg tcttttctat ttcccaggca gcccttatct
     1501 ggttccattt ttgtcttagc gtccttttgg aatctttagg ttttagatct ttatctagtg
     1561 ttgatgcaag ctgcatagtt attgtactag ctcctctatt gtttttagat acgagactct
     1621 caaagatggc aacctcgatt gctctccaat ctactccatg gtgatcataa aaccgtttat
     1681 cttctgaatg aatgactgcc ttgattaaag actgggaaat atctttaagg cttatccact
     1741 caagcctcct tcccttttca tctactctta gttcgtggat gatttctccg tgtctatcca
     1801 gaagaaccgc atcggatttt ttgtaagaat ttttaacttc atcaaatgtg ggtatggcat
     1861 tggcgtaggt tgtaaagaga tatgagaatg tcaggatcaa taacgacgtt ttccatcggg
     1921 atgacgggct aataatagca ttagtccagc agcaagtctt cgtctgaagc ctaggcgaat
     1981 tggaccggtg gactatctgt tttattatat ttacacgtcc attaatttgg gcacgactct
     2041 tactcacggc cgcttcaaac aaattcgcga agaaaattct catcatatca gtggtgatca
     2101 ttttattacg acgatactta cggctcaacc ctaatctcct tattcggcat ttcccccagc
     2161 atctcaggag aataaagcgc ctcaacccta gttggaggaa gaccgagtac tccctcgtta
     2221 ttaagtctca aggtgtattc agcagtccat tttccttttg gtacgtattc ataatatgtc
     2281 ctaaatgctt cgaatgagcg ctcttcgaat gcaggccaaa cccatccttt actctcttct
     2341 ccttgtgtta aaacctccga gtctctacta agcccagtcc ctaaaatagc tgagccggca
     2401 ggtattggat catttacaac gacccatgtc atatcggctt gcgcctcaag ctcaaggcga
     2461 atcctcatta catctccagt gctccatttc cccgctattt tttgttcgac gggtataaga
     2521 gtttttttaa tcttataccc acttgagaac ggttctttaa gcggaattgc agcaaggctc
     2581 tctattgtag cccagggttt cccagagccc tgatgaacga tgctcaattc ttctttcgta
     2641 tctggccagc taaacattcc actcttgcct ttaggggatt ccttccaatt aactgtcttt
     2701 gttgttccat acaattcggc agatgtagag cctgtcacag gaatggattc aaatttctcc
     2761 gagaacctcc ccattgcaag cactccccat gcatttgcaa cagttgtatc ccagtgtcct
     2821 cgtttctgtc ttccgataac gccccttgcc attctgggta tgtcatcttt ccagttctca
     2881 aatgaaataa gagttaataa cccccttgca gcattttcat cggttgagat cattagccac
     2941 cagaggtagt ctgtgctttc agttgaaaaa ctcattgtag ttccccggag attaagccgg
     3001 gaccttatta tctgttcgga ttcctttagt tttttatcac gatctggaat atctttaatc
     3061 ctcaaaagta cattcatcca atctatcacg gctgatgtag gccataaatt aggctctatt
     3121 gatatagaac ctagaaggtt tgggtcactt tcaccattac gtgaaagggc ttctaataca
     3181 gccatctttc ttattgaaag gtctgctgtt ggtaaagacg aatatctaat tacccttcct
     3241 tcaataaacc cttttaatcc atctatcatc cggtttttta attcggatgg gatttcatat
     3301 ttcgcctcac ttgaaattga gaggatatag gaagtcagca catcacttcc aaggtccatt
     3361 ttggggaaat attttactag cccatctgaa tcaagatatg aaggtaactc agccataatc
     3421 tttttccaca acaactcgtc ccttaatgct atagcccgtg acaccctttg ctccgtacac
     3481 gtatagggat agcttttcat gtaacggata actccatct
//
LOCUS       DATDRL010000203         2684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3030359, whole genome shotgun sequence.
ACCESSION   DATDRL010000203 DATDRL010000000
VERSION     DATDRL010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3030359"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VI935_06975"
     CDS             complement(<1..1073)
                     /locus_tag="VI935_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLE25379.1"
                     /translation="MIYSLKQLGFPPADDQIEITVFGPGYGESIVVYIPGLGWGVIDS
                     CRYEFKNTTYILPLEYLKAYNVTSLCFVILTHPHADHYRGMGEILRHFDSSIKRICRY
                     SGKGIREFKTYLTELESQKGDRQGDIRELMKLFKLMGYLQNEKGVKLRRLSELTEIIP
                     AKNVSLKNHSKVRVRLISLSPSSASEEEYERLLDQTTVKAIVEGDLARLLDLNDRRHN
                     LIASALLLEIGTIQIVLGSDLERGDSNQTGWKGVCSNVDCPNLSAHMVKVPHHGSHAA
                     HYDQAWNAHSSSGRLVSVVSPYNKSSPPLPRISDINRIKKYCIQVGLTSYSGLRRADE
                     AYSREVRRAIGNNIRKSWLISSG"
     gene            complement(1070..1600)
                     /locus_tag="VI935_06980"
     CDS             complement(1070..1600)
                     /locus_tag="VI935_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25380.1"
                     /translation="MKISSGTLQSKSLQEASPTLVGKEDYKTETSVLYSYKDSAFPAS
                     SAIKNGETTKLDYKPFSKEFSPQDTNEVILEWWIGEVDKVYNDYFSAYMIDLKGKEIG
                     AEFDMNSVDPNSRELLKPRAQFAYYVSRIDSIKGRRTASALVFSKPFRLSYESKEALL
                     KRAEELFGPEPIFDEE"
     gene            complement(1597..2325)
                     /locus_tag="VI935_06985"
     CDS             complement(1597..2325)
                     /locus_tag="VI935_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25381.1"
                     /translation="MGEETQTSPLYQCSIVILGSFNPGIFQPEWIERFGILPEQEVKA
                     VIEPGATVTLSDGKTKAPAFIITPEITQILFPTFQLKVGLSRFEFLTLQRDEFERVPE
                     VVIKIFHLLPHTPISAVGINFNIHLPIKDDVNEILKSYFQGKFFTEESSFSDGNNLLQ
                     ILGESYEIWGTFRCKKEEDTVTVQVQPSLRIENGIYLDYNFHSDLQDKTAKELVDILT
                     KNFLTDFEIARKIQYFISREESGL"
BASE COUNT          810 a          530 c          461 g          883 t
ORIGIN      
        1 tttcctgaag agattaacca tgactttctt atattgttgc caatagcccg cctaacttcg
       61 cgactgtacg cctcatccgc tcttcttaat ccagagtatg atgtaagtcc aacttgtata
      121 caatactttt ttatcctatt aatatcacta atccgtggta aaggaggaga actcttatta
      181 taaggtgaaa cgacggaaac taacctgcca ctagaactgt gggcattcca cgcttggtca
      241 taatgcgccg catgcgaccc gtgatgtggt acctttacca tatgtgctga taaattgggg
      301 caatcaacat tagaacagac acctttccat ccagtctggt tagaatcgcc tctttctaga
      361 tcgctgccta aaacaatttg tattgttcct atttcgagca gtagggcaga tgcaattaag
      421 ttatgtcgcc ggtcattcaa atccaataat ctagctaaat caccttccac tattgccttc
      481 acggtggttt gatcaagcaa cctttcgtat tcttcctcac tcgccgagga gggagagagt
      541 gatattagcc taactctaac tttactatga ttctttaggg acacgttttt ggcaggtatt
      601 atttcagtca attcagaaag tcttcttaat tttacgcctt tttcattttg caagtagccc
      661 attagcttaa ataatttcat taactcccgg atatcccctt gcctatcacc tttttgagat
      721 tctaattctg tcaaataagt tttgaactct cggattcctt ttccactgta acgacatatt
      781 cgcttaattg atgagtcaaa atgacggagt atttctccca taccccgata gtgatcagca
      841 tggggatgag taagtataac aaaacataaa ctggttacat tataggcctt cagatactcc
      901 aatggcaaaa tataggtggt atttttgaac tcgtaacgac aactatcaat tactccccat
      961 cctaaacctg gaatataaac aactatagat tcaccatatc cggggccgaa gacggtaatt
     1021 tctatctgat catccgctgg gggaaatcct aactgcttta gagagtagat cattcttcgt
     1081 caaaaatagg ttcgggaccg aatagttctt cagctctctt tagaagagct tcttttgatt
     1141 cgtatgataa acgaaaaggc ttagaaaaaa ccaaagcaga tgctgttctc cgccctttaa
     1201 ttgaatctat acgactaaca taataagcaa attgggctcg cggttttaaa agttcgcgtg
     1261 aatttggatc aactgaattc atatcaaatt ctgctccgat ttctttacct tttaaatcaa
     1321 tcatgtatgc gctaaaataa tcattgtaca ctttatcgac ctctccaatc caccattcta
     1381 aaattacttc gttagtgtct tgaggagaga attctttaga aaaaggttta tagtcaagtt
     1441 tagtagtctc tccattttta attgcactac tagcagggaa ggcagagtcc ttatagctat
     1501 ataagacact agtctctgtt ttataatcct ctttacccac aagagttggg ctagcttctt
     1561 gcaaagattt ggattggagt gttccagaac tgattttcat agtccactct cctctcttga
     1621 aatgaaatat tggatctttc tcgctatttc aaagtccgtc agaaaattct tagtgagaat
     1681 atcaactaat tctttagctg ttttatcttg taagtcacta tgaaaattat aatctagata
     1741 tataccgttt tctattctaa gagaaggttg aacctgaacg gtaactgtat cttcttcctt
     1801 tttacagcga aaagtacccc aaatctcata ggattcaccc aaaatctgca gtagattatt
     1861 cccatctgaa aatgatgact cctctgtaaa gaatttacct tgaaaatagg atttcaatat
     1921 ttcatttaca tcatctttaa ttggtaaatg tatattaaag ttaataccta ctgcactgat
     1981 tggtgtgtgg ggtaacaaat gaaatatttt aattactact tccggtactc tttcgaattc
     2041 atcacgttgc aatgttaaaa attcaaacct agagagtccg actttcaatt gaaatgtagg
     2101 aaagagtatt tgagttattt ctggtgttat aataaaagct ggggctttgg ttttaccgtc
     2161 tgaaagagta acagtagccc caggttctat aaccgctttt acttcctgct caggtagaat
     2221 tccaaaccgc tcaatccact cgggctgaaa tatgccggga ttgaatgaac cgagaataac
     2281 aattgaacat tgatataatg gactagtttg agtttcctct cccatgcctt tttatcgaag
     2341 tttacacggt tgatgatata ctaacaagaa tcggatgttt tgcgtaagct atttatatca
     2401 taaataatag ctcaatgtca acaaaaattc tattgaatcg ttcatcgtaa gaacacggaa
     2461 ctatgaatgt taataatatt aaaacccttt tgtattaaaa caaatattga tatttgttga
     2521 aatggtctcg aagattattt aagaaaactc acccaagctc gtagtagtca taatattcta
     2581 atccaaggtg ggtaataagc tcttctcctt ttagatagcg gagggtgttt tttaatttca
     2641 taagttgaat aaaaagatca tgttctgggt aaagcccttc accg
//
LOCUS       DATDRL010000204         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2148197, whole genome shotgun sequence.
ACCESSION   DATDRL010000204 DATDRL010000000
VERSION     DATDRL010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2148197"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VI935_06990"
     CDS             complement(<1..439)
                     /locus_tag="VI935_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLE25382.1"
                     /translation="MAVKKYKMFVDGKWVSSHTGETWDVINPANQEVIARVPLADEED
                     VRMAIMAARRAFDKGPWRKMSQVERGKLLLALARAIRDQAEELAQLETLNSGKPIRES
                     RMDISDAADCFEFYGGLADKINGDIVPVPDPNIFAMVLREPVGV"
     gene            complement(740..2104)
                     /gene="gabT"
                     /locus_tag="VI935_06995"
     CDS             complement(740..2104)
                     /gene="gabT"
                     /locus_tag="VI935_06995"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HLE25383.1"
                     /translation="MKKTSEYLKTEIPGPKSKGMIELRGKYVPRGVFNTVPAFIERAK
                     DALITDIDGNTFIDFASGLATVNVGHSRKEITEALRVQIEKYLHTCFHVFMYEPYVRL
                     AEKLAEITPGDFPKKAMFVNSGAEAVENAIKIARYFTKRKAVLTFEHAFHGRTLLTMT
                     LTSKLRYYKLGFGPFAPEVYRMPYAYPYRCPFGADYSNCAVACLDHFKSFFKTHVDPE
                     EVAAVIIEPVTGEGGFIVPPPEFLPGIDRICKENGIVFIVDEVQTGFGRTGEMFAVNH
                     YNLEPDIIVMGKSIAAGLPLGAVTGRTEIMDAPEVGSLGGTFGGNPLSCVASLKVIEI
                     MQEDGFMEHARKVGNIINVKLKDIQERYAIVGDARGLGAMAAIELVKDRFTKEPAKEE
                     TTEVLKQCHKNGLVIMKAGAYDNVIRLLVPLIIDEDMLQRGLDILENAVRAVNKGYSS
                     KKKV"
     gene            complement(2418..>2725)
                     /locus_tag="VI935_07000"
     CDS             complement(2418..>2725)
                     /locus_tag="VI935_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HLE25384.1"
                     /translation="AKIIHEGSQRKDKAFIDIDCSAIPETLIEAELFGYEKGAFTGAK
                     PEGKPGLVELATGGTLFLDEIGELPQNVPVRLLKFLDHMEFTRLGGTKKRKVDAMVI"
BASE COUNT          750 a          671 c          467 g          837 t
ORIGIN      
        1 ctacaccaac aggttctctg agcaccattg caaagatatt tgggtcggga acaggaacga
       61 tgtcgccatt aattttgtct gcaagaccac cataaaattc aaagcaatct gccgcatcac
      121 tgatgtccat tcttgactct cttatgggtt tcccggagtt taaggtttca agttgagcaa
      181 gctcctcagc ctgatctcta atcgcacggg caagggcaag tagtaatttc ccgcgctcga
      241 cttgactcat ttttctccaa ggacctttat caaatgcgcg tcttgctgcc attattgcca
      301 ttcttacatc ttcttcatcg gcaagtggga ccctagcaat cacctcctgg ttagcaggat
      361 ttataacatc ccaagtctct cctgtatgag aagaaaccca tttgccatca acaaacattt
      421 tatatttttt caccgccata ggtatcacac ctcaaattta gttgttagcc gtcagtcagc
      481 agtcttgtac ttaccgtcat ttcgagctca gcggggcaat ctgacacaaa gtgtcattcc
      541 gaatgcttcg catatctcag tacaggctcc aggaggaatc tcaatcagat tgcttcgtcg
      601 ctttgctcct cgctatgaaa ggtaattagc ctcgttaatc aaagtgacaa aatcatatgc
      661 tgttttacag cgcattataa tcatctaccc aaagactaac gactttacaa aagattataa
      721 caatttctca tgcctacttc tagactttct ttttcgagga atatccctta ttaactgctc
      781 gaactgcatt ttctaaaata tctaaacctc tctgaagcat atcctcatct attattagag
      841 gaacaagaag ccttataacg ttgtcataag cgcccgcttt cattattacc agaccgttct
      901 tatgacactg tttaagcacc tcagttgtct cttccttagc gggctctttt gtgaatctat
      961 ctttaacaag ctcaatagcc gccatagctc ctaaccccct ggcatctccg actatggcgt
     1021 atctttcctg tatgtcttta agtttcacat ttattatatt gcccacctta cgggcatgct
     1081 ccatgaatcc atcctcctgc attatctcta ttacttttaa cgaggcaaca cacgatagcg
     1141 gatttcctcc gaatgttcca cccaacgacc caacttctgg tgcatccata atctctgtcc
     1201 tccccgttac tgctccgaga ggaagccctg cggctattga tttccccata acgattatgt
     1261 caggttccaa gttgtaatga ttaacggcaa acatctcacc ggttctccca aaccctgttt
     1321 gcacctcatc aacgataaaa acgattccgt tctccttaca aatcctatct attccgggaa
     1381 ggaactcagg aggtggaact atgaatcctc cttcgccagt gacgggctcg ataataacag
     1441 ccgcaacctc ttcaggatcc acatgtgttt taaagaagct tttaaaatgg tcaaggcagg
     1501 caaccgcgca attcgaataa tcagctccga acggacaacg gtatgggtaa gcataaggca
     1561 tacggtatac ttcaggtgca aagggaccaa atcccagttt gtagtatctt aatttgcttg
     1621 tgagtgtcat agttaggaga gttcttccat gaaacgcatg ctcaaaagtt aaaactgcct
     1681 tccttttagt gaagtatctt gctatcttta tcgcgttttc gacagcctca gccccactgt
     1741 ttacgaacat agcctttttg ggaaaatcac ctggagtaat ctcagcaagc ttttcagcaa
     1801 gcctaacata aggctcgtac ataaacacat gaaaacaggt atgaagatat ttttctatct
     1861 gcacccttag agcttcggtt atttcctttc ttgagtgtcc aacattaaca gttgcaagac
     1921 cgcttgcaaa atctataaag gtgtttccat ctatatcggt gattagggcg tctttggccc
     1981 tttcaataaa tgcaggcacg gtattaaaca ctcctcttgg tacatatttc cctctgagtt
     2041 caatcattcc ttttgatttt ggtcctggaa tctctgtttt tagatattca cttgtttttt
     2101 tcattcctat accccataag caatggaata gattttaaat ctttggtatt tatagaatcc
     2161 tgcgatacta accataattt tatatttatg aaaagtaaat agcctatctg tctcgcaagt
     2221 tacacttcgc atatgctcat cacaggcttt gtcagatagg tatctgacat taagtgatat
     2281 tctgtacaaa gtaagcaatc acgatcagag aactttaaat tacactgcaa acaacgaaaa
     2341 taaattgtat taagattgta cagcaattac tatgccgtga taggcttcta tcggaactaa
     2401 ctgaaatcat tagaaaacta aattaccatt gcgtcaacct ttctcttctt tgtaccgcct
     2461 agcctagtaa attccatgtg gtcaagaaac tttaaaagcc ttactggaac attttgtgga
     2521 agctccccta tctcatccaa aaaaagggtc ccaccagttg caagctcaac caatcctggc
     2581 ttaccttcag gctttgcccc cgtaaatgca cccttttcgt aaccgaatag ctcagcttcg
     2641 ataagggtct ctggtattgc actacaatct atatcaataa acgccttatc cttgcgctgg
     2701 ctcccctcat ggattatctt tgcaa
//
LOCUS       DATDRL010000205         2282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1620699, whole genome shotgun sequence.
ACCESSION   DATDRL010000205 DATDRL010000000
VERSION     DATDRL010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620699"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VI935_07005"
     CDS             complement(<1..489)
                     /locus_tag="VI935_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HLE25385.1"
                     /translation="MKISELLDKNTIELDLKAKKKVQAIQEVADILYKANKIKNPKNI
                     AAVLLEREKISPSTFENGVAIPHATIEGIKEVVACLAISRSGIDFESPDKRPVRVLFL
                     FLSPLEESETHLQILSKAEGLLQNKKLCDALITSYSKEKLINEIINAERMGWDMYINL
                     SKE"
     gene            797..1408
                     /locus_tag="VI935_07010"
     CDS             797..1408
                     /locus_tag="VI935_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLE25386.1"
                     /translation="MIVKNCMTSNPVTVIPEEDVKNTSHLLQKYRIRQTPVVKDGRLV
                     GIVTDRDLRMALIRPNLTVGDVMSSSPVTVIEDAPVEEAARIICNRKFNALPVVSKTG
                     ELVGIITVTDILDNLIHFLGFHEEPFRVEVKVSEGVNLYGVIKVLQGNTEKVVSFSLS
                     RERGVTFYFWVIGCDFDKLDRKLQEKKLDVAVNYPEGLTKLAQ"
     gene            1641..2192
                     /locus_tag="VI935_07015"
     CDS             1641..2192
                     /locus_tag="VI935_07015"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HLE25387.1"
                     /translation="MFLLFLACDSGLPVKGELPDLPLKDQDGKKFTFSQLRGKVLIVS
                     YTYTHCPDICPMTIKKMKEIEDVLKKSGFQDSVYFVSISLDPKRDTPEVLREHARTNN
                     SDLRNWVFLTGNEVIINSIMKSAGVAAINEPTQYSENKEPYYFITHTDRISLVDKKGR
                     IRKHYKGSSFDIKEVLENIERLL"
BASE COUNT          723 a          383 c          435 g          741 t
ORIGIN      
        1 ttctttcgaa aggttaatat acatgtccca acccattctc tccgcattga tgatctcatt
       61 gattaacttt tccttgctat aactggtaat aagggcgtcg cacagtttct tattttgcag
      121 aagcccttct gcttttgaga gaatctgaag atgggtctca ctctcttcta atggagataa
      181 aaagagaaat aaaactcgaa ccggtctttt atccggggat tcgaagtcta ttcctgacct
      241 agatattgcc aaacaggcaa caacctcttt tattccctcg attgtagcat gtggtattgc
      301 tactccgttt tcaaacgtag atggtgaaat cttttctctt tctaaaagta cggcggctat
      361 atttttgggg tttttaatct tatttgcctt ataaagaata tcagctacct cttgtatcgc
      421 ttgcaccttt ttctttgcct ttagatctag ttcgattgta tttttatcta atagttcaga
      481 gatcttcatt ctttcaatac cggttttgat tttgtctaaa ctctattata tgaactatga
      541 ttgtaaagtg ttatagttta tctatagatt aaaattgtta tttaatcgtt acttattact
      601 attttttact caattcttat atttccttgc aaaaataaga ccgaataaat aaacgcagaa
      661 atccttctgt aagattttct tacatcttac acatcttata aatccaatcc ctttaattca
      721 tatattatat caatggtaca attcttgctt tttaagaaaa aagaacactt ggaagaatga
      781 aaagaggtga aaaaacatga ttgtaaaaaa ctgtatgacc tcaaaccctg tcacagtaat
      841 ccctgaggaa gatgttaaaa atacctctca tttgttacaa aaataccgta tccggcagac
      901 ccctgtggtt aaagatggaa ggctcgtggg aatagttaca gatagagacc ttaggatggc
      961 tcttatacgc cccaacctta cagtaggtga tgttatgagt tccagccccg tgaccgtaat
     1021 agaggatgca ccagtagaag aagccgctcg aataatttgt aaccgcaaat ttaatgcact
     1081 tcctgttgtt tctaagacag gtgaactggt agggattata actgtcactg acattcttga
     1141 taatttgatt cattttttag ggtttcacga agagcctttt agagtggagg ttaaggtatc
     1201 tgaaggtgtg aacttatatg gtgtaattaa ggtgctacaa gggaatacag aaaaggtagt
     1261 atcgtttagt ttatctagag agcgtggtgt tacattttac ttttgggtta tcgggtgtga
     1321 ctttgataag ttagatagaa aattacaaga gaagaaactt gatgttgctg taaattatcc
     1381 cgaaggctta accaaacttg cacagtgata tactagagag gaatagggta taatagataa
     1441 tctttccata ttataatctt aactgaactt gtaatataat gtttccaaag ggatataagg
     1501 ctaagtgaaa acgacgcaac aggctgtgca ttcttttagc tcatatcact gtccaaatca
     1561 agccttgaaa cgatctcagt gtctacacta tggcagtgaa cttattccgg tttgaaatga
     1621 aaggatttta tatatttttt ttgtttctgt tgttcttggc ttgtgattct ggattaccag
     1681 taaagggaga actaccagat ttacccctaa aggatcagga tggaaagaaa ttcacctttt
     1741 ctcagcttag agggaaggtt cttatagtaa gttataccta cacacattgt cccgatatat
     1801 gccctatgac aatcaagaag atgaaggaga ttgaggacgt cttaaagaag agcggttttc
     1861 aagatagtgt ttatttcgtt tctatatcac ttgaccctaa aagagataca ccagaggtat
     1921 taagagagca tgcccgaacg aataactcag atcttagaaa ttgggtcttt cttactggga
     1981 atgaagtcat tataaattcc ataatgaaat cggctggagt agcggctata aatgaaccca
     2041 ctcaatactc tgaaaacaaa gagccttact atttcataac ccatactgat aggataagcc
     2101 ttgttgataa aaaggggagg ataagaaagc actataaagg gagttccttt gatattaaag
     2161 aagttttaga gaatatagag aggctcttat agatcaaata tcctttcttt cgaaaataaa
     2221 aagagctact agtagtggaa ttaaaagcca agcaaggagg gctattgagg aaattatcat
     2281 gc
//
LOCUS       DATDRL010000206         2011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1811381, whole genome shotgun sequence.
ACCESSION   DATDRL010000206 DATDRL010000000
VERSION     DATDRL010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811381"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VI935_07020"
     CDS             complement(<1..191)
                     /locus_tag="VI935_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25388.1"
                     /translation="MNIDVLLNAFEKMASNYPELSLDEAKGVLKQYLLKRKDFDVQDE
                     AIIEALLKDKDRLLEQSFI"
     gene            320..1744
                     /locus_tag="VI935_07025"
     CDS             320..1744
                     /locus_tag="VI935_07025"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HLE25389.1"
                     /translation="MTNTRIAYGDEFISARLPERTQILEPPSPLPLLKNLEQAIRDAL
                     YSPIAHDPISKLVGPKSKVTIAFDDPVIPQVPMKKPDFREMAITILLEELEKAGVKRS
                     NIKLICANALHRKFTNGELSTILGSKITLGFGPSHLYCHDAEDKDNLVLTGETERGFE
                     VEVNKAVMESDQVFYVNITSLPFHGGWKSIVVGLSSYRSIRQHHRPFRGASGKSVMDA
                     KRSSFQKLIWEMGAVVERELAINGRRIFTIESVLNTTQPQELIAVFAGNISDVHEKTL
                     ELLYRQQVISLSKQTDVAIYGIPNQSYYANLSKINPILVRNQALSYAFGLYHNKPLIR
                     EGGIAIFVNPCLAEFNEMNHPSYVEFFNRVLPHTQDPYDIWDFYAEDFAHRPEYVHKY
                     RYAYGFHGVHPLILWGQGAFPLRHLGRVFLAGAKDFETARRIGFEPFGTLDEAIAEAE
                     ASLGKDCSITYHPAPPAFITSVEV"
     gene            complement(1769..>2011)
                     /locus_tag="VI935_07030"
     CDS             complement(1769..>2011)
                     /locus_tag="VI935_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25390.1"
                     /translation="RLDPHLTILKIYLAKTLMKQKKYEEAKKILKEVIEEKNPSVYAD
                     WYLNKKMTEKMIFKIEKKIQRGSSPPYPLGNASRLK"
BASE COUNT          576 a          407 c          410 g          618 t
ORIGIN      
        1 tctataaacg attgctcaag aagcctatcc ttgtctttaa gaagcgcttc aattatcgcc
       61 tcgtcttgaa catcaaagtc tttccttttt aataggtact gctttaatac tccttttgct
      121 tcatctaatg aaagctcagg atagttactt gccattttct caaaggcgtt aagtaaaaca
      181 tcaatgttca tgttaatccc tctcatttag taatataccc aaaataaccg tatgtatatt
      241 taatagaacc tgcatttaat attgactact actcatgtct atataaaaat tgttgtaaca
      301 actcccgagg aggtaaaata tgacaaatac aaggattgcg tatggtgatg agtttatttc
      361 tgcaagactc ccagagagga cccaaattct agaaccgcca tctcctcttc cactactaaa
      421 aaatctagag caggccattc gagatgcgct ttattcccct atcgctcatg atccaatctc
      481 aaagttagta ggtcccaaat cgaaggtcac cattgctttt gatgatcctg tcatacctca
      541 agtacctatg aagaaaccgg attttagaga aatggctatt acaattcttc tcgaagaatt
      601 agaaaaggct ggagtaaaaa gaagtaatat taagcttatt tgtgctaatg cacttcaccg
      661 taagtttaca aacggagaac tgtcaacgat tttaggttct aaaattacct taggatttgg
      721 acccagccat ctctactgcc atgatgctga agacaaagat aatcttgtac tgactggcga
      781 aacggagcgg gggtttgagg ttgaggtaaa taaagctgtt atggagtcag atcaggtttt
      841 ctatgtaaat attacgagtc ttccttttca tggcggatgg aaatctattg ttgtaggact
      901 tagcagctat cgaagtatac gccagcatca tagaccattt cgaggagcaa gcggcaagtc
      961 tgtaatggat gccaagcgct catcttttca gaagcttatc tgggaaatgg gtgcagttgt
     1021 tgaaagagag ttagctataa atggaaggcg aatttttact attgaatccg ttttaaacac
     1081 tactcagcca caagaactaa tagcagtatt tgcggggaat atttcggatg tgcatgaaaa
     1141 aacgctcgag ctactctaca ggcagcaggt gatttccctt tccaagcaga cggacgtagc
     1201 tatatatggt attcctaacc agtcttatta tgcaaacctt tctaaaatca accctatttt
     1261 agttaggaac caggcccttt catatgcatt tggactctac cataataaac cccttattcg
     1321 tgagggcggg atcgctatct ttgtaaatcc ctgcttagca gaatttaatg agatgaacca
     1381 cccatcatat gtggagtttt tcaatcgagt tcttccacac acacaggatc catatgatat
     1441 atgggacttc tacgcggagg attttgcgca caggcctgag tatgtacata agtatcgcta
     1501 cgcctatgga tttcacgggg tccatccact tatactctgg ggtcagggag cgtttccact
     1561 tcgtcacctg ggcagggttt ttcttgccgg ggcaaaagac ttcgagaccg caagacgtat
     1621 aggttttgag ccctttggaa ccttagatga ggcaattgct gaagcggaag caagccttgg
     1681 aaaggattgt agtataacct atcaccccgc cccaccggcc tttatcacat ctgtagaggt
     1741 gtagaggact tgagaaataa aggaaaattt atttcaatct ggaggcattc cccaggggat
     1801 aagggggaga cgagcctctt tgaattttct tctctatttt gaaaatcatc ttttctgtca
     1861 tttttttatt taggtaccaa tcagcgtaaa cagaggggtt tttctcttct ataacttctt
     1921 taagtatctt ttttgcctcc tcgtatttct tctgtttcat gagagttttt gcaaggtata
     1981 ttttcaagat tgtaaggtgt gggtcaagcc t
//
LOCUS       DATDRL010000207         5225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_918486, whole genome shotgun sequence.
ACCESSION   DATDRL010000207 DATDRL010000000
VERSION     DATDRL010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918486"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VI935_07035"
     CDS             complement(<1..371)
                     /locus_tag="VI935_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HLE25391.1"
                     /translation="MNSEERMAFILDHYENPRNHGARDNADVVQKWGNPGCGDIVTIY
                     LNFDDKGHITNVTFEGEGCTLSQAATSIITEMAIGKTLEEVESMSPNVISDIVGLEIA
                     MNRPKCATLGLTSVKMAISEWR"
     gene            complement(561..1406)
                     /locus_tag="VI935_07040"
     CDS             complement(561..1406)
                     /locus_tag="VI935_07040"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HLE25392.1"
                     /translation="MAKYVHPEVLVSTEWVSEHLSDPTVRIVESNEDILLYDMGHVPG
                     AVKTDWQGELQDQLIRDYINVEKFEKLMSEKGIANDTAVVFYGDKNNWWACYAFWTFK
                     LFGHEKCMVMNGGRQKWIAENRPLTKDVPTYPRTTYKITQVNESIRSFRDNVTKHING
                     KKPLIDVRSPKEYTGELLHMEAYPQEGALRGGHIPGAKNVPWARAANEDGTFKSANDL
                     KTIYQGEIGLKRGDNIIAYCRIGERSSHTWFVLTYLLGYKKVRNYDGSWTEWGNLVRA
                     PIEKP"
     gene            1655..2131
                     /locus_tag="VI935_07045"
     CDS             1655..2131
                     /locus_tag="VI935_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLE25393.1"
                     /translation="MIENLKVRDIIGKREKIFSVDADDTTDIAALKIRNFKVRTIGVI
                     KNGKLVGVIGQSDFSNKVVAMSKRPSDLTISEIMSTDLVTVGLDSSFFDCVRLLDTHH
                     ISHLVILDEKGNYYGMLSWWDLQQKLVEELKNQLELLQEYAFGPHRNVLLDQIIRK"
     gene            2229..3002
                     /locus_tag="VI935_07050"
     CDS             2229..3002
                     /locus_tag="VI935_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLE25394.1"
                     /translation="MTYKNIELFEDDWFAMIVMNRQARRNALSLDHMKELVHAFRAVG
                     GGKCRGVMLAANGPVFSAGHDFSDMAGSNLNEMRALLNTCTELMTTMQSIPQPVIARV
                     HGLATAAGCQLVATCDLAVASEKASFATPGGKGGWFCTTPMVAVSRAIGRKRALEMLM
                     TGDPIDAQTAADWGLVNKVVPAERLDEETRSLLERATRGSVLSKGMGKQAFYAQIDLD
                     QPKAYAYAVEVMAASALTEDAQEGMRAFLEKRQPVFKGR"
     gene            complement(3047..4216)
                     /locus_tag="VI935_07055"
     CDS             complement(3047..4216)
                     /locus_tag="VI935_07055"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HLE25395.1"
                     /translation="MTSKELGESPLVIVEHGSVGLVETKYYTFSYPPNKFVLDNGEKL
                     GPITVAYETYGELNETKDNAILIEHALTASAHVAGKRSPDDKHPGWWDVMIGPGKAFD
                     TNKYFVICSNILGSCYGTTGPSSIDPETGKPYGMTFPLIGIRDMVRVQKELTQYLGVN
                     RILSIAGGSMGGMQAIEWALFYPEMVDSIILIASAPRSTPQSIAIHRVGLQAIMDDPN
                     WNNGDYYGKEPPNKGLAIARMIGHITYLSDEWLWQKFGRRHTDPETMKLRLDSKFEIE
                     SYLEYQSSKFVQRFDANSYLYIMRSIDIYDAAEGYTSLEESFNRIRCKKVLVSSFSAD
                     WLYPAYQSEEIVKALIENNIVFTYSKIESSYGHDSFLLEHERLTHLITTFLDSLE"
     gene            complement(4394..>5225)
                     /locus_tag="VI935_07060"
     CDS             complement(4394..>5225)
                     /locus_tag="VI935_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HLE25396.1"
                     /translation="GNPLINVLDIEAVASIAHEAGIPLIVDNTFATPYLCRPFEFGAD
                     IIVHSATKFICGHGTSIGGVVVESGKFPWDNGNFPVMMEPSKAYHGVRFYETFGDFGY
                     IMKARVETLRTFGPALSPFNSFLFIQGLETLYLRMERHCSNALAVAKFLKEHPAVTWV
                     NYPGLTDNPYHELAKRYLPKGAGSIFTFGIKGGKEAGVRFIESAQLLSHLANVGDAKT
                     LVIHPASTTHRQLTDEEQLTAGVTPDMIRISVGLETIDDILWDIEQALEKSQKAKIAI
                     "
BASE COUNT         1389 a         1105 c         1129 g         1602 t
ORIGIN      
        1 cgccattcgc taatagccat tttaacactg gtaagcccta aagtcgcgca ttttggacgg
       61 ttcatcgcta tttctagtcc tactatatct gaaatgacat taggactcat tgattcaact
      121 tcctcaagtg ttttaccaat agccatttca gtaattatag aggttgcagc ctggctaagc
      181 gtacatccct ctccttcaaa tgtaacgttt gttatgtgac ctttatcatc aaagttgaga
      241 taaatcgtca caatatcccc acagcccggg tttccccatt tttgaaccac atcggcgtta
      301 tcacgtgcgc cgtgattcct agggttttca tagtggtcta gaataaacgc cattcgttct
      361 tcactgttca tggccataag agtttacctt gaagttatct tcttataaat aactgttgag
      421 ctacaaagac actaagatat taagttagaa tccagaagcc atgagtcaaa agtcagtaat
      481 gacgcaagat tttgcgtctt ttcttctgga ttctgacttc tgattccttc cttttgccaa
      541 cgtgactggt tacattctaa ttaaggtttt tcgatgggcg ctcttacaag attcccccac
      601 tctgtccatg acccatcata gttcctgacc ttcttgtaac ctaaaagata tgtcaaaaca
      661 aaccaagtat gtgaagacct ttcaccaatt ctacagtaag caatgatatt atctcctcgt
      721 tttagtccta tctcgccctg atatattgtt tttagatcat tagcggattt aaaggttccg
      781 tcctcattag ctgctcgagc ccagggtacg ttcttagcgc ccgggatgtg cccgcctctc
      841 agcgctcctt cttggggata agcctccata tgaagtaatt cccctgtata ttccttgggt
      901 gaacgtacat caataagagg cttttttcca tttatatgct tcgttacatt atcacgaaat
      961 gaccttattg attcattaac ttgtgttatc ttataggttg tccttggata tgttggtacg
     1021 tcctttgtca gtggacggtt ctctgcgatc catttctgtc tgccaccgtt cataaccata
     1081 cacttctcat gtccaaagag cttaaatgtc caaaacgcgt aacacgccca ccagttattt
     1141 ttatctccat agaaaacaac agcagtatca ttagcaatgc ccttttcaga cattagcttc
     1201 tcaaacttct ctacgtttat ataatcacgg ataagctggt cttgaagctc tccctgccaa
     1261 tcagttttaa cagctccggg gacatgtccc atgtcataga ggagtatgtc ctcattggat
     1321 tctactattc taacagttgg gtcacttagg tgttctgaaa cccactccgt gctgactaaa
     1381 acctctggat gtacatattt tgccatgtct acctccttta catttaagtt tttatttttg
     1441 acataagtct atgaaatgct tcaaccattg taatatatgt aattcaagaa agcaacaaaa
     1501 acagatgcaa acagatacac tcatgaactt agcctgtata tccgtattag aactgcaggt
     1561 ttaattcttg ttcttttttt cttcaatttg tgatattcta aaaacatcac ccgttgtcaa
     1621 tgggggtaac aattatctac agggggtggc atccatgata gaaaatttaa aggttaggga
     1681 tataattggt aagagggaga agatattttc tgttgatgct gatgatacta ctgacatagc
     1741 tgcgttgaag attaggaatt tcaaggtaag aactatagga gttattaaga atgggaagtt
     1801 ggttggagtt ataggccaga gcgatttttc aaataaggtt gttgccatga gcaagaggcc
     1861 gtcggatttg actatatctg aaattatgag cactgattta gtaactgttg gactcgattc
     1921 ttccttcttc gactgtgtaa ggcttttgga tacacaccat atttctcatt tggtaatact
     1981 tgatgaaaaa ggaaattatt atggaatgct atcatggtgg gacctgcaac agaaattggt
     2041 agaggaatta aaaaaccaac tagagctact tcaagagtat gcatttggac cgcatcgcaa
     2101 tgtactatta gatcaaatta taaggaaata accattaaac aaaatagtgc tatttaggaa
     2161 gtgggccata tgggttagtt taaattcatt gtaggtgcct caatttattt cctaggcggg
     2221 gagttataat gacatataaa aacatagagc tttttgagga tgattggttt gctatgattg
     2281 tgatgaatcg ccaagcgcgg cgaaacgcac tttcactcga ccatatgaag gagcttgtac
     2341 atgcatttcg cgcagttggt gggggcaaat gccgtggggt tatgcttgct gctaacggtc
     2401 cagtattttc ggccggtcat gacttttctg atatggctgg gagtaaccta aacgagatgc
     2461 gagccctcct taatacctgc accgaactga tgacgacaat gcagtcaatt ccacagcctg
     2521 ttattgcgag ggtacacggt ttagcgacgg cagcgggctg ccagcttgtt gctacctgtg
     2581 acttagctgt tgcttcggaa aaagcatcct ttgctactcc tggtgggaaa ggtgggtggt
     2641 tttgcacaac gccgatggtt gccgtaagcc gcgctatcgg tcgaaagcgg gcccttgaga
     2701 tgctgatgac aggagatcca atagatgccc aaacagcggc tgactggggc cttgtgaata
     2761 aagttgtccc tgctgagcgg cttgatgaag aaactcgttc tctccttgag cgtgctacgc
     2821 gtgggagtgt gctctctaag ggtatgggca agcaggcgtt ctacgcccaa atagacctag
     2881 accaaccaaa ggcatatgca tatgcggttg aggttatggc tgcgagcgca ctcactgagg
     2941 atgctcaaga gggtatgaga gcgtttctag agaagcgcca gcctgtgttt aagggtcggt
     3001 aagccattgt ttgcgttttc attatagcca atataatgca ctacatctat tcgagtgaat
     3061 ccaaaaaggt tgttataaga tgggtgagcc tttcatgctc aagtaggaac gaatcatgtc
     3121 cgtaggagga ttcaatttta ctgtaagtga agacaatgtt gttctcaata agtgctttaa
     3181 caatttcttc cgattgatat gcgggataaa gccagtctgc cgagaaggaa gatacaagaa
     3241 cctttttaca tctgattctg ttgaatgatt cttctaagct tgtgtacccc tctgcggcat
     3301 cgtagatgtc aattgaccgc atgatgtaaa gatagctgtt agcgtcgaat ctctggacaa
     3361 acttggagct ttgatattcg aggtagcttt ctatctcaaa cttgctgtct aaacgaagtt
     3421 tcatcgtctc tggatccgtg tgtcttcttc cgaatttttg ccatagccac tcgtcgctta
     3481 gataagttat gtgaccaatc attcttgcga ttgcaagccc tttgttaggc ggttcttttc
     3541 cataataatc accgttgttc cagtttgggt cgtccataat ggcctgaaga cctaccctgt
     3601 gaatcgcaat agattgtggg gttgaccttg gggctgaggc aatgagaatt atagagtcca
     3661 ccatttcagg ataaaaaagc gcccattcaa ttgcttgcat ccctcccatt gaaccgcctg
     3721 caatgctcag aatccggttg actccaagat attgggtaag ctctttctgt acgcgtacca
     3781 tatcacgtat tccgattaga ggaaatgtca tcccgtaggg ttttcctgtt tctgggtcaa
     3841 ttgatgaggg ccccgttgtg ccataacagc ttccgagaat attcgaacaa atgacaaaat
     3901 acttattagt gtcaaaagcc tttccgggac caatcatgac atcccaccag cccgggtgtt
     3961 tatcgtcagg gctgcgttta cctgcaacgt gggcgcttgc tgtaagggca tgctcgatta
     4021 gaattgcatt atctttagtt tcgtttaact caccgtaggt ttcataagca accgtaatcg
     4081 gtcccagttt ctcgccatta tcaagaacga atttattcgg tgggtatgaa aaagtatagt
     4141 atttagtttc aacaagcccg acagagccgt gctcgacaat aactagaggt gattccccga
     4201 gttccttgga ggtcatgcct ttaccttttt gaacattagc cactaatgaa cacgaattta
     4261 caggaataaa atgatgtacg atgaatgatt catgatacaa gattgatgaa ggtttttact
     4321 tgcatcctgt attttgtatc ttggatcctg catcctgttt ttttcatgcg tgctcattcg
     4381 tgttaattcg tggctagata gcaatttttg ctttctgtga cttttctaag gcttgctcaa
     4441 tatcccagag tatatcatca atagtttcaa gtccgactga tattcttatc atatcggggg
     4501 taactccggc ggttaattgt tcttcatctg taagctggcg gtgtgtagtg gatgcaggat
     4561 gaataacaag cgtttttgca tctcccacgt ttgcaaggtg actcaagagc tgagcgcttt
     4621 caataaatct aactccggct tctttgccac ctttaattcc aaatgtgaat atagagccag
     4681 cgcccttagg aaggtatctt tttgcaagct catgatatgg gttgtcagta agtccaggat
     4741 aattaaccca agttactgcg gggtgctcct tcagaaattt agcaacggcc aaggcatttg
     4801 agcagtgcct ttccatccga agataaaggg tttccaagcc ttgaataaat agaaatgaat
     4861 taaaagggct aagtgcgggt ccgaatgttc taagcgtctc cacccttgcc ttcattatgt
     4921 agccaaagtc cccaaatgtt tcatagaacc tcacaccatg ataggccttt gaaggctcca
     4981 tcataacggg aaagtttccg ttgtcccagg ggaatttccc agattccact acgacgccac
     5041 ctatagacgt tccatggccg cagatgaatt ttgtggctga gtgcacaata atatcggctc
     5101 cgaactcgaa aggtctgcat agatacggag tagcaaatgt gttgtcaaca ataagcggaa
     5161 ttcctgcttc atgtgcaata cttgcaacgg cctcaatatc aagaacgtta attaatgggt
     5221 tgcct
//
LOCUS       DATDRL010000208        18045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1068711, whole genome shotgun sequence.
ACCESSION   DATDRL010000208 DATDRL010000000
VERSION     DATDRL010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 18045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18045
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068711"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VI935_07065"
     CDS             <1..984
                     /locus_tag="VI935_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HLE25397.1"
                     /translation="CYYKIQGHFSNRWDEVDIASRGILLDSLKDYPQFAYIAFLGIDT
                     YSHLNHPFHHKVIESYFRIDESVGILAKALKDMGRLDETLLIITSDHGLTPTHSHFDS
                     VSFMNRLGYKTFHYPKIFKYYRSADAANMISGNAMTHLYVKSSQGWEKKSTFEELTQL
                     VDGLLERQEVDIVAGLDENGRVRIKSQRGEASTWLDKDDFIRYERFDADPFGYNGIPD
                     RLNREDALKYSINTKYPDALLQIIQLLESPRSGDLVVSAKQGFDLRAKHENPEHCSSH
                     GALFREQILVPLGINIKTKKEFVRTVDLYPTILHSLGKPIPKGTDGVSLVD"
     gene            complement(992..2953)
                     /locus_tag="VI935_07070"
     CDS             complement(992..2953)
                     /locus_tag="VI935_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HLE25398.1"
                     /translation="MNISLLNPFFLIGLITVSLPIVVHLISRKSGIKKSFSAVSFLFS
                     SQVETLRRSKIKDLILLIIRSLILVSLVLVFSKPNVFSLSYVDVNDVKTVAIVIDNSF
                     SMGYGDNFKIAKNKAKSLIESLADGSFGAVVPLVPTDITERVVTKDKRKMLEVLENIK
                     LSFSFTNNERRLEEIFSFIVKAPNNKKEVFLFSDFQRNGWINEKFKREWLLPIDVTSG
                     SQMQNRAISDLDVKDEGDATKVSVRGSNYSNSPVDDLLATLFIDNKEIKGFFRIEPWS
                     EQIKEFIVPKDTLNSDQATGKVEIPHDNLKVDDVRYFVHSQSKEPRILLVDGDPREDT
                     RLSETYYLARAIETISEIFPLKLSIIDNDSFLSEKLKLKDYDLLFMSNVGDISTKKAL
                     DIEEFLRDGGVAVIFLGDRVRNDLYNVMLRNVLPAEIGAISQGDYFLNANELNQFTDE
                     MNEKFRQVEVKRVFDLHPVKDTNIILSTSYNKPYLTQRRLENGNIFLFSSTSDTAWNN
                     FPITPVFLPVIKRALDLYLSKQPSIKGLLVGESLEIDFPNDIDEVKIGTPSGEEIKVL
                     RANPNFSHTLIPGIYAVKVGDKALYNFSANIDPRESNLEKISLESISPRSGIERGFVK
                     VLKEIWIYFLWGAIALFISESIIRFVFSR"
     gene            complement(3049..3921)
                     /locus_tag="VI935_07075"
     CDS             complement(3049..3921)
                     /locus_tag="VI935_07075"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HLE25399.1"
                     /translation="MPKDIVDIKAALRLKGFSFRISSRVKGIKAGIHKSQHKGISPDF
                     LEYKEYSYGDELKHIDWRLYGRHDRLYVKKFEDEVNLRWCILIDRSGSMGYASNGNNK
                     LDYAIRLSATLAYLLLKQGDAVGIGDFSDNDLNMITPKTGIANLTPIVEKLNSFNAYG
                     NTELKEPILRAIEKTKTDSAFVIVSDFLMDLDKIEESIKLLRASCREVIAFQVLDTNE
                     VEFNFDGSIEFIDMEGEKKILVDSGDIRDAYRKRMKEFIERLKFICLKHKSRYVFSPT
                     ILPIEDVLIQIVEK"
     gene            complement(3961..4455)
                     /locus_tag="VI935_07080"
     CDS             complement(3961..4455)
                     /locus_tag="VI935_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HLE25400.1"
                     /translation="MFKIGDIAVYPVYGVVMVKGIEVKEICGTKKTFYIFKVVDSDVT
                     VMVPTDNTHTVGVRPIISKAEIPRIYQILRTRKRTNNSTNGHKSWNKRYREYAEKLKS
                     GNIFEVAQVLKDIYLLQNEKELSFGERRIMNAARTLLIKEISISKDLEEESIAGEVEK
                     LLVE"
     gene            complement(4649..5257)
                     /locus_tag="VI935_07085"
     CDS             complement(4649..5257)
                     /locus_tag="VI935_07085"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLE25401.1"
                     /translation="MRKVISKVVFSFGMLSLSIIPIANSSYADFYSYVDQKGVHHYTN
                     IPPTDKRYKLKWRTKKASVKPNGIYAYSKSYEEEILRAAKRHNLDPDLVKAVIKVESN
                     FNSSAISRKGAIGIMQLMPDTADDYNVSNPFNPKENIDGGTKYLKKLMEMFSGDLELA
                     LAAYNAGENAVIKYGFRVPPYSETINYVEKVLMHYNNLKEKY"
     gene            5513..5893
                     /locus_tag="VI935_07090"
     CDS             5513..5893
                     /locus_tag="VI935_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25402.1"
                     /translation="MLFSFSYSQSKGNDEETLSKIKALEIKLSGIQSKSTVIPTEEVN
                     KASEWIEEAKKSFNSGRPGVTQIIMEKASYQVEYLNALIEESRVKREVEEKKEFLQKI
                     RSQTEELKAINKEVENEIKGFEDK"
     gene            5890..6939
                     /locus_tag="VI935_07095"
     CDS             5890..6939
                     /locus_tag="VI935_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLE25403.1"
                     /translation="MRIVILFIVTLTLASASKAAPTVKDIMSAYNLLEDAKRQGGGAY
                     APQAMKEAQFFYEQAERELKNGNDERAEEFRLISEIRSKTAISISRKKTYENELQVIE
                     FQLDETSSIKKNFEGELRKNVTRLEEIKEKLAISQDRMLSTALDTLEKASERINAAEG
                     VSAEDFNPQALDEARQTYSIAEEDLTLGEYKKSIELGEKAIDLAEIAYERSKTKFELR
                     SGILDRVSRIYGAKTEPLKDGVKVNLHGVFAPSGKTILFDAFPSLDALASVLAGYSNL
                     PVTIEVYASEQNSDDENIKIGETQAETVKNYLVSKGISPQRFKVTSAGLGVKEGVKED
                     SRIEVIIHLFELSST"
     gene            complement(7186..7575)
                     /locus_tag="VI935_07100"
     CDS             complement(7186..7575)
                     /locus_tag="VI935_07100"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HLE25404.1"
                     /translation="MKKVLILLLITSLPLFWACAGPDETGSVSLDGPILESINQEGNL
                     EFNGAVVNSGDTPVNFVEVVLILKDEDGKVVEASFTSVIGEDTQSLLQPSERAFFTIT
                     AKSDPKRIASKEVEIFSEEASDLPPSS"
     gene            complement(7572..8324)
                     /locus_tag="VI935_07105"
     CDS             complement(7572..8324)
                     /locus_tag="VI935_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25405.1"
                     /translation="MRLKNKLGTIATALGVELCILAFVGLVFLFQFDKAKVELVNKTK
                     AISETIGEQASSFFSQRMKAGTSDEFFQFLDQRLGRKKLFNTFDVAPKFFSVVLRRDV
                     ESGKVADDIKDDFYPKKGYTVSNQHGMISVSVPFAIQGQSEPFGIVKIDSDTQAIRAK
                     VLADNSLLYAAILVVLNNQVFILYLLLRRRKEVIFEKGYLREHSVGALKIMYRVLGDI
                     IQDHEGAGEPSAKQGESKKVISISELVEKRKK"
     gene            complement(8315..8728)
                     /locus_tag="VI935_07110"
     CDS             complement(8315..8728)
                     /locus_tag="VI935_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLE25406.1"
                     /translation="MRFGDYLKNKRRQKNTTQEDLARALGVSSVFIHQLETGKVDAPS
                     LERCNQIADILEVTIDELWNVAKKERLRRFMEREGITEDDFEVLTEGERTLIKLYRSL
                     DEDSRRDFGGMVYMLLRRSQDEGVQEILEEFMKCA"
     gene            complement(8994..9134)
                     /locus_tag="VI935_07115"
     CDS             complement(8994..9134)
                     /locus_tag="VI935_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25407.1"
                     /translation="MLEAQGPAGKSHIIQADDPKTLKGIRIGDNVNVNIEEEIAVSVE
                     KI"
     gene            complement(9916..9988)
                     /locus_tag="VI935_07120"
     tRNA            complement(9916..9988)
                     /locus_tag="VI935_07120"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9953..9955),aa:Phe,seq:gaa)
     gene            10463..11506
                     /gene="pilM"
                     /locus_tag="VI935_07125"
     CDS             10463..11506
                     /gene="pilM"
                     /locus_tag="VI935_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HLE25408.1"
                     /translation="MVFGKKEIVGLDIGSSAIKLAELREARNGYQLKNIGESLLPPEA
                     IVNKVIINSGAVVETISTLVKDLGVKTKDVAISISGHSVIIKKVSIPQMSEKELREAI
                     PWELEQNIPHSIDNVNYDFQILPGESQEGNMDVLIVAAKKDITEDYIAVTNEAGLNPV
                     VVDVDVFALENMFEINYPVTDEVTALINIGASVTNINILKGGVSIFARDITAGGRQFT
                     EWIQKEFNIGYDEAEKMKYSLGLTGVSKELDRITRDFTELICGEIERTIDFFATSLKE
                     PVRKIFLGGGSSKVPYIKEQLQERIGSSVEMVNPFKNILHDVRDFDPEYILDISPKMG
                     VAIGLAIRRKGDK"
     gene            11503..12117
                     /locus_tag="VI935_07130"
     CDS             11503..12117
                     /locus_tag="VI935_07130"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HLE25409.1"
                     /translation="MIKINLIPVKEGKEIKGLRDLIIGVIAIIAVFILLVALQLWRIK
                     QIKDVNIKIADVKARILKLEDVKKKVEEFKAKNKELQEKIEVIKVLEENRTGPLYVME
                     ALGKAIPNRAWIDKLSEKGSSAKMEGFAWDELTVSDFMKRLQSFPYFQNVELKVINTK
                     EIQQLPLKAFVIESRLNYSGKVKPEEKPQEIPEVKKPSQQKAQR"
     gene            12120..12776
                     /gene="pilO"
                     /locus_tag="VI935_07135"
     CDS             12120..12776
                     /gene="pilO"
                     /locus_tag="VI935_07135"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HLE25410.1"
                     /translation="MAIIENISDALNRQRLYVRLGILIGIIVIMCGVFWYFFWSPKTE
                     ELKSARNELQRNEAKLREYEAIAKELPKFEQEFKRLNREFEEASRKLPKEKEIPTLID
                     SIYAAVSASGLESNTFAPKPEVKKTIYAEIPIEMKVYTSYYDLAKFFNRVSKLPRIVN
                     IRDLNLSQDKKKSTGQKIVLNAAFTAVTFRLLPPQPVQPDTKTDTKRKKATKKTEPKE
                     "
     gene            12773..13261
                     /locus_tag="VI935_07140"
     CDS             12773..13261
                     /locus_tag="VI935_07140"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HLE25411.1"
                     /translation="MNGGAQGIRKGLILFLLIGLWVFVSHAQEDQKSATDFKTIQEGS
                     TQKPSVNRDPFRPFIKIVEKKEVETEVGKSVPPIKRYPLEQFRLVGIVWVGEQPKVMV
                     VDPEKNTYVLGVGDEIGSRQGKIIEVRESGIMVEEKNYFEDVFGQKKVESVKSVLAFK
                     GE"
     gene            13303..15996
                     /gene="pilQ"
                     /locus_tag="VI935_07145"
     CDS             13303..15996
                     /gene="pilQ"
                     /locus_tag="VI935_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HLE25412.1"
                     /translation="MKLILKAVFAGFFLFVLVSFLNSESSGLVTQANDNKDTHGVIAE
                     FNNETVKESPLLPKTNKSFASQSSVVNSTNKRHIATGSNKVESIGFGVQGLIGIVTIK
                     TTVPVKFEELPKGEKRLNLKLLDVFLPEEFQVSRDVNEFGSPVSFISTFKDLAGEGDV
                     IVIVELKEDARTELNQQDNVLTLRFTKQSGEETIKVSEKTQVEESQPTSNQSAPIEGV
                     AADSKQAEPAEKIEESPPTTIKAPETHPTIQTSSSKEAVNPVIKQEEATKQVEESKAP
                     QKTQIAESQPSTQSNSSKETVDTSYKEENTPKVIEGDVLPTTTLMVESQPSTQSFPSG
                     APGSASLNTVESIGFEVKEGVGIVAIKTSAPVTYEELPKEEKKLNINLQDVFLPEEFQ
                     VSRDVSDFDSPVRFISSFRDPVIARRVIIVLELKEDAPVVVKQEGNFITVNIGTPKGE
                     EEITDGLQPVPEGPLYAFDFRIATTPGAKKVYRGQLVSFDFKDADVRDVLKILADISG
                     FNFIVSRDVKGTVTLKLSNVPWDLALDIVLEDATLGAVVEGNVIKVAPLKTIQAKQIA
                     IRSAAQSKEQLEPLITKQIFINYATADELTPLADPLLSQRGDIKVDTRTNSILVTDTA
                     SRVRQIEELVKSLDTRTPQVLIESRIVQATLDFTRELGVQWGVDYKASAATGNPTGAT
                     FPSSINAGGTNIGAPFGTQGNNFIVDLPAAVGTGSGGAIGIVLGSLSGAFDLDIRLSA
                     LENRGDGRVLSSPRVLTLNNNPARIEQGVSIPFLSVSAAGTQTQFVDANLTLDVTPQV
                     TNDNRILMDIKVTDNRPDPTLTGANGQPSIRRNEAETQTIASDGETIVIGGIYTRETS
                     ESLRGIPWFNRIPLLGWLFRTERNIDSRRELIIFVTPRIVK"
     gene            16012..16578
                     /locus_tag="VI935_07150"
     CDS             16012..16578
                     /locus_tag="VI935_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25413.1"
                     /translation="MEKPEILGIALFPALVISTLIIALSCGPNPPFAPQGSTITILNP
                     LDDITIPPESLQPTFRIQAFVTDPDGNALNSVRVRFSLSFADQNSLVVDTDGDGESDS
                     RALQLVDNSNCNPNPCDLTPISQWFQQGAFVDSPYTELSNDRGIAEIIILIPGPDVVI
                     VDPAVFEASLDNGSPPDNFQFSVNAGAQ"
     gene            16619..>18045
                     /locus_tag="VI935_07155"
     CDS             16619..>18045
                     /locus_tag="VI935_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25414.1"
                     /translation="MKRLIKTSDSNNKLRQFLSILTVFLVFVGLNCVNGLDQGSSDDP
                     AHTGIIFVNGVPVVIFGNSIDQSLIEVTTGVFPNGSTIEFEITLSDLIELLSGCVFGG
                     DVIIVDGQAVANYLAGILIGTPDQLSSGDPPVAALNVAVKITTPDGDSESDFVTMFLN
                     AVGMIPPEDMDVTTSPDDGITLPVIVTLVFQTIGIPPGTNVSFSLSNPALGMLNPLVT
                     PVSGAEDNGEAETLYTTVNGSSGIQTITATIILPNPFDFDPSCPNVPVGQRTIQVSVK
                     INQIAPVPTPTPSPSPTPGPTPTPSPTPTPGPTPTPSPTPGPTVTLVCPDQIVEGDQA
                     QCTCSTNQGQGVQLCFVVSDPPLTLISPTMPDCDTTDGAGEAFVVVLAQDVMGAEIGQ
                     VECCLSLGDDMCDPGDLNDVEPITVVPVPTPTPTPSPTPGPTPTPTPSPTPTPGPIMI
                     TAGDLTPAAGGQTVIEAFSNQNVDDL"
BASE COUNT         5557 a         3383 c         3639 g         5466 t
ORIGIN      
        1 tgttactata aaattcaggg acattttagt aatcgctggg atgaagtgga tatagcttca
       61 agagggattc ttcttgattc tctgaaggat tatcctcaat ttgcctatat tgcttttttg
      121 ggtattgata cctattcaca tctaaaccat ccgtttcacc acaaggttat cgaatcatac
      181 tttagaatcg acgaaagcgt aggaatatta gcaaaggctt taaaagacat gggaagactg
      241 gatgaaaccc ttctcataat cacaagcgac catggtctta cacctacaca ttcgcatttt
      301 gactcagtgt catttatgaa ccgtctcggt tacaagacct ttcattatcc taaaatattt
      361 aaatattata gaagtgccga tgctgctaac atgatttccg gtaatgctat gacccactta
      421 tatgtgaaaa gctcccaagg ttgggagaag aaaagcacct ttgaggagct cacccagctt
      481 gttgatggtc ttctcgaacg acaggaggtt gatatagtgg ctggtcttga tgaaaacggt
      541 agggttagga ttaaaagtca aagaggagaa gcttcaactt ggctagataa agatgacttt
      601 attcgatatg agcggtttga tgctgatcct tttggttata atggtatacc tgatagattg
      661 aatagagaag atgctcttaa atatagcatc aatacaaaat atccagatgc gcttctacag
      721 attatccagc tcctcgaatc tccacgctct ggagatttag tagttagtgc aaagcaaggt
      781 tttgatctta gagcaaagca tgaaaaccct gaacactgct catctcacgg agcgctattt
      841 agggaacaga tacttgtccc ccttggtatt aatataaaga caaagaagga atttgttaga
      901 acggttgatt tgtatcctac gatacttcac agtcttggaa aacctattcc caagggtacg
      961 gatggggtga gtttggtaga ttgattcacg cttaccttga aaatacaaat cttataattg
     1021 actctgaaat aaataaagca atagctcccc atagaaagta gatccatatc tcttttaaga
     1081 cttttacaaa tcctctttca atacctgatc gaggagagat ggattctaag gaaatcttct
     1141 caaggtttga ctccctagga tctatgttcg cggaaaaatt atataatgcc ttatcaccca
     1201 cttttacagc atagattcca ggaataagag tgtggctaaa attaggattt gctctaagca
     1261 cttttatttc ttctcctgag ggggttccaa ttttcacttc atctatatca tttgggaaat
     1321 caatttccaa agattcacca actaacaagc ctttaatact aggttgtttt gataaataca
     1381 agtctaacgc tctctttata actggaagaa aaacaggtgt aattgggaaa ttattccaag
     1441 cggtgtcaga tgtagaggaa aaaagaaata tatttccatt ctctaacctt cgttgagtta
     1501 agtaaggttt attatatgag gtgcttaaaa taatatttgt atcttttacc gggtggagat
     1561 caaataccct ttttacttct acttgtctaa atttctcatt catttcgtct gtgaattgat
     1621 ttaactcatt agcattcaga aagtaatcac cttgtgatat tgctcctatt tcagctggga
     1681 gaacgttcct aagcattaca ttatacaaat cgtttctaac tctatctcca agaaatatca
     1741 cggctactcc tccgtccctt aaaaattctt cgatatcaag tgcctttttt gtggatatat
     1801 ctcctacgtt cgacatgaat aatagatcat aatcctttag ctttaacttt tcagaaagaa
     1861 atgaatcgtt gtctattatt gatagcttta gcggaaatat ctcagagatc gtttcgatag
     1921 ctctggcaag ataataggtc tcactcagtc ttgtatcctc cctcgggtca ccatctacaa
     1981 gcaggatccg cggttctttt gactgagaat gaacaaagta cctaacatcg tctactttta
     2041 gattgtcgtg agggatttca acctttcctg ttgcttgatc cgaattaagg gtatctttgg
     2101 gaactataaa ctcttttatt tgctcgctcc agggttcaat cctgaaaaag ccctttatct
     2161 ctttgttatc aataaataaa gtagctaaca gatcgtctac cgggctattt gaatagtttg
     2221 atcctcttac tgaaaccttg gttgcatcgc cctcatcttt aacatctaaa tcagatatag
     2281 cacggttttg catctgagaa cctgatgtga cgtcaatagg taaaagccat tctcttttaa
     2341 atttctcgtt aatccatccg ttccgctgaa aatcggaaaa taagaatacc tcttttttat
     2401 tattgggtgc tttaacaata aaactgaaaa tttcttcgag tctcctttca ttattagtaa
     2461 aagagaagga tagttttatg ttttctaaaa cctccaacat tttcctttta tcctttgtaa
     2521 caacacgttc tgtaatatca gttggaacaa gggggacaac agctccgaaa ctcccatcag
     2581 caagggattc tattaacgat tttgctttat tcttggcaat tttaaaattg tctccatagc
     2641 ccatgctaaa cgagttgtca atcacaatcg caacggtttt aacatcgtta acatctacgt
     2701 aagataaaga aaaaacatta ggttttgaaa acacaagaac cagactaact aggattaagg
     2761 atctaataat gagtagaata aggtccttta tttttgatct tcttagtgtt tctacctgtg
     2821 aagaaaaaag aaaactcact gcagaaaaac tcttttttat accacttttc ctagatatta
     2881 gatgtacgac gatgggaagg gaaaccgtta tcaggccgat taggaaaaat gggtttagga
     2941 gagagatgtt cacttctttt ccatattgta accatttagc cggcaataaa atgacacatt
     3001 atgcacgatt caatataaat gaaaattcca tctatcctgt atcctgcatc atttctcaac
     3061 aatttgaata agtacatctt cgattggaag gatagttggt gaaaacacat atcgtgattt
     3121 gtgtttaagg catataaatt ttaatctttc aataaactct ttcatccttt ttctataggc
     3181 gtctcttata tccccagaat cgacgagtat cttcttctcg ccttccatat ctatgaactc
     3241 aatcgaacca tcgaagttaa attcaacctc gttggtatcc agcacctgaa aagcaatgac
     3301 ttccctacaa gaagctcgaa gaagtttaat agattcctct attttatcta aatccataag
     3361 aaaatctgag actattacaa atgcggaatc cgtcttagtc ttctcaatag ctcttagtat
     3421 aggctccttc agttcagtat tgccatacgc attaaatgaa ttaagttttt ctactatagg
     3481 agtaagattc gcaatgcctg tctttggtgt aatcatattt aaatcgttat cagaaaaatc
     3541 tcctatccct actgcatctc cctgttttaa aagcaaatag gctagtgttg cagaaagcct
     3601 tattgcataa tcgagtttat tattcccatt agatgcataa cccattgaac cacttctatc
     3661 gatgagtata caccatctta gattcacttc gtcctcaaac tttttaacat aaagacggtc
     3721 gtgtctaccg tatagtcgcc agtcaatatg tttgagttca tcaccatagg agtactcttt
     3781 atattcaaga aaatccggac ttatcccttt atgctgactt ttatgaattc ctgcttttat
     3841 accttttacg cgggaagaaa tacgaaaaga aaagcccttg agccttaagg cagcttttat
     3901 gtcaacgata tcttttggca tgattcaagg gatcaagaaa agtaactaca agaattctac
     3961 ttattcgaca aggagttttt caacttctcc agcaattgac tcttcttcca agtcctttga
     4021 aattgagatt tctttaataa gcagcgtgcg tgcggcattc attattcttc tttctccgaa
     4081 agaaagttct ttttcatttt ggagaaggta tatgtctttt aatacctggg ctacctcgaa
     4141 tatgtttccg ctctttaatt tttcggcgta ttcacggtat cttttattcc aactcttatg
     4201 accgtttgtt gaattgttcg ttctttttct tgttctaagt atttgataga ttcttgggat
     4261 ttccgctttt gagattatag gtcttacgcc aacagtatga gtgttatcgg taggcaccat
     4321 tactgtaaca tcactgtcta cgaccttaaa aatgtaaaag gtctttttag taccgcatat
     4381 ttccttaact tcgatccctt taaccatcac aacgccataa actgggtaaa cggctatatc
     4441 accgatcttg aacattagct cccttccttg gcgttaattt aattaatact atcacaattt
     4501 ataatcaggg tcaacagaaa aaacatttgt ttactaactc aatgataaag agcggaacta
     4561 tttttcttat ttaaccgctc ttgttacatt ttattgaaaa ctgttataaa acagagtact
     4621 caattgattc aaggttttcc atatactttt agtatttttc ttttaaattg ttataatgca
     4681 ttaaaacttt ttcgacataa ttaattgttt cactataagg aggaactctg aatccatatt
     4741 ttattacagc gttttcacct gcattgtaag cggctagggc aagctcgaga tcaccgctaa
     4801 acatttccat gagcttcttt aaatatttcg tgccaccatc aatattttct tttggattaa
     4861 atgggttgct tacattgtaa tcgtctgcag tatctggcat aagctgcata attccaatag
     4921 caccttttcg tgaaattgca cttgagttga agttcgattc tactttgatt acagccttta
     4981 caagatcagg gtctaagtta tggcgttttg cggctcttag aatctcttcc tcataggatt
     5041 tcgagtaggc ataaatgccg tttggtttta cagacgcttt ctttgttctc catttaagtt
     5101 tatatctttt atctgttggg gggatgtttg tataatgatg aacgcctttt tgatctacgt
     5161 aagagtaaaa atccgcataa gatgaattcg ctattggaat tatcgaaaga gaaagcattc
     5221 caaaagaaaa aaccactttc gaaataacct ttctcaatac catggccacc gttattttat
     5281 cactattaat gtgaaatgcc tagagtgata ttaaaaattt taaaaatatt aagttattat
     5341 atacgacgca tgctctatct tgaccaattt tcttattgaa tatagtatct tataaaatta
     5401 tttgcattgt tactgtattt aagcgttttg gaaaaggtgc caaaaaatta gaatttatat
     5461 tagactttat ctttttggaa tgaggactat tatagcaaca acgattttat tcttgttgtt
     5521 ttcattttct tattctcagt ctaaagggaa tgatgaggag acactaagca aaataaaagc
     5581 cctggagata aaactttctg gtattcaaag taaatcaacc gtgattccta ctgaagaggt
     5641 taataaagca tcggagtgga ttgaggaagc aaagaaatct tttaactctg gtaggccagg
     5701 ggttacacag ataataatgg aaaaggcctc ttatcaagtt gagtacctaa acgctctaat
     5761 agaggaatcg agggtcaaaa gagaggttga agaaaagaag gaatttcttc aaaaaatcag
     5821 gagtcaaaca gaagaattaa aagcgataaa taaagaagtt gaaaacgaaa ttaaggggtt
     5881 tgaggataaa tgagaatcgt tattcttttt atcgttactc ttaccttagc tagtgcatct
     5941 aaggcagcac ctactgtcaa ggatattatg agtgcgtaca atcttttgga agatgcgaaa
     6001 aggcagggtg gtggagcata tgctcctcag gctatgaagg aggcgcagtt cttctatgag
     6061 caagcagaga gggaacttaa gaatggaaat gatgaaaggg cagaggaatt tcgactgatt
     6121 tctgaaataa gatcaaagac tgcaatatca atatcaagaa aaaaaaccta tgagaatgag
     6181 cttcaggtaa tagagtttca gttagatgag acaagttcca taaagaaaaa ctttgagggg
     6241 gagttaagaa aaaacgtcac taggcttgag gaaattaaag aaaaactggc tatttctcag
     6301 gatagaatgc tttcgaccgc acttgatacg cttgagaagg cttcagagag aataaatgcc
     6361 gctgagggtg tgtctgccga agattttaat ccccaagcac ttgatgaggc gcgtcaaacc
     6421 tacagtattg cagaggagga tttaacttta ggtgaataca aaaaatctat tgagcttggt
     6481 gagaaggcca tagacctagc tgaaatagca tacgagcgat caaagacaaa atttgagctc
     6541 agaagtggta tcttagacag ggtttccagg atatacggag caaaaacgga gcctttgaag
     6601 gatggtgtaa aagttaacct tcatggtgtc tttgctcctt caggaaaaac cattctattt
     6661 gatgcatttc cttctctaga tgcactggca agtgttttgg ctggatattc aaatcttcca
     6721 gtgaccatcg aagtatatgc aagtgagcag aactccgatg acgaaaatat aaaaataggg
     6781 gagacccagg ccgaaactgt aaagaactat ctagtttcca aaggaatttc cccccaaaga
     6841 tttaaggtga cgagtgctgg cctcggtgtc aaagaaggag ttaaagaaga tagtcgtatt
     6901 gaggtaatca tacatctttt tgagctcagt agtacgtaac cttctgataa gaaataatct
     6961 tttaataatg acaacttaaa aaatacatgc cggtttaact gtttaataaa ctcagttgaa
     7021 tcggataata atgattgaag ttaatgaaca tcgttatatc tgcctcgatg aaaaataaac
     7081 cgatcttgta gttctgagct tgagacatgt tgattcgata aacaaggtta ttctcggatc
     7141 ctctaataat tatttcgtgt gagtggatgt atctctagat gattgctacg aagaaggtgg
     7201 taggtctgaa gcttcttcag aaaagatttc tacctctttt gacgctattc ttttcgggtc
     7261 tgattttgca gttatagtaa agaaggctct ttcagatggc tggagtagac tctgggtatc
     7321 ttctccaata accgatgtga aacttgcctc gacgaccttt ccgtcttcgt cttttagtat
     7381 caaaacaact tcgacaaagt taactggtgt gtctccggaa ttaactacag caccgttgaa
     7441 ttcgaggttg ccttcttgat ttatgctttc taggatcggc ccatctaggc ttacagaccc
     7501 agtttcatct gggccagcgc atgcccaaaa taggggtaac gaggttatta gcaataatat
     7561 aagaactttc ttcatttctt tcttttttca acaagttctg aaattgaaat taccttcttg
     7621 ctttctcctt gtttcgcgct tggttctcca gcgccttcgt gatcttgtat tatatcgcct
     7681 agaactctat acatgatttt cagtgcacct acggaatgct ccctaaggta tcccttttca
     7741 aagataacct cttttcttct ccttagtagg aggtaaagta taaaaacctg attgttaaga
     7801 actacaagaa tagcggcgta taaaagggaa ttgtcggcaa gtacttttgc cctaattgct
     7861 tgggtgtcgg agtcaatttt tacaattccg aagggctcag attgaccttg aatggcaaaa
     7921 ggaacggaaa ccgatatcat tccatgttgg tttgatacgg tatatccctt tttagggtaa
     7981 aaatcgtcct taatatcatc tgctaccttt ccactttcga catcacgtct taaaacaacg
     8041 ctgaagaact ttggtgccac atcgaatgtg ttgaagagtt tttttcttcc gagtctttga
     8101 tctaggaatt gaaagaactc atcgcttgtt cccgctttca ttctctgtga aaaaaaactg
     8161 ctagcctgct caccgatagt ttcagaaata gccttcgttt tattgacaag ctctaccttc
     8221 gccttgtcaa actggaaaag aaatacaagt ccaacaaacg ctaagataca aagctctact
     8281 ccaagtgctg tagctatcgt tcctaatttg ttttttaagc gcatttcata aactcttcga
     8341 gtatttcctg aacgccttcg tcttgagagc gcctcagaag catgtatacc attcctccaa
     8401 aatcgcgtct tgaatcttcg tcaaggcttc tataaagttt tataagcgtt ctttctccct
     8461 cagtaagaac ctcgaaatca tcctcagtga tgccttctct ttccataaat cttctaagtc
     8521 tttctttctt agcaacattc catagctcat caattgtgac ttctaaaata tcagctatct
     8581 ggttacatct ctctaaggat ggtgcatcaa cctttcctgt ttcaagttga tgaataaata
     8641 cgcttgagac gccaagcgcc ctagcgagat cttcttgggt ggtattcttt tgtctccttt
     8701 tatttttcaa atagtcgcca aatctcattg gatgacctcc ggagaatcaa gatttccttc
     8761 ctaatacaaa aaattactat aaatattata acaattctat aaaatagcaa gggattttgt
     8821 tgcaattgaa attccggtat tgtggattct tgaatactaa attgctcctc aataccttga
     8881 ccagtctttt aaatgaggtt gggaaatatt atctaaaatt tttcccttct gatcttgaat
     8941 tcaaaataca tggttattgt acatagatag ataactcagc tccgatagat gatttaaatc
     9001 ttctcaactg aaacagcaat ttcttcttcg atgtttacat ttacattatc accgatcctg
     9061 attcctttaa gggtcttagg gtcatcagcc tgaatgatat ggcttttacc tgctggtcct
     9121 tgagcttcaa gcaatcgcct ttagcttgag aattccctgt agcttgctac agggtttcca
     9181 caaaagtgta ttgtcattgc gaacgaagtg aagcaatctc tcattttaga ttgcttcgtc
     9241 cccttcgctt tgctcagcgc ttcggctcac ttgctcctcg caatgacagc tataacgtta
     9301 tatttttaag accatacccc tgcagcttgc tgcaggggta ttgattgtac agaaccaaac
     9361 cttgaatctt ggattgcgcc tgatctgcta gacttgtgct ctacttcttt atcggattat
     9421 tacagtgtct tattcctgca ggtgcgtata taccaataag ggtcaaccct gctgctatga
     9481 gtatggataa aaggaatctt catcacctag tttcacgcct gtgaagtgtt tatttagtca
     9541 tgcttgcaat atttatactg agtcaatatc cataatattt agtcgttaaa ggaagttaca
     9601 aaaagaaggc tgataggatc tgtataaatg tgtgatatta ctctgtgaac aatgtggaag
     9661 tttacacagg aattttacct tggggaacta ataaatttgt gttgaaaaaa cattcttttt
     9721 ttgaatttta ccgaaagaaa tgaataaaga aagcctattg aataatgtga atccataaaa
     9781 cctgtcctgt tattttctag ttgtttaagg tcaattgagc ttattttata atatttagga
     9841 aaaaataaat ccaataacat gatgaaaagt gttataattc cctgtcttga ctcacttttg
     9901 taaagaaaat aagcatgccc ggagacggaa tcgaaccgcc gacacaagga ttttcagtcc
     9961 tctgctctac cgactgagct atccgggcat tttaatgttt tcagtatatt acaactcacg
    10021 gattaggtaa aaattgaagc ttataaagac ctagtaataa attgggggaa attctaccta
    10081 accactaccg cttatgcaag gtaggtatta cctgtctgaa taatactttt aagtataggt
    10141 attgacaaca atagagtttt tgaaataaga tacaatacag ataagattga agccaagaat
    10201 cgaaagtact tgaagtaagt gaatatctaa tggttattta agagcaatca aggagttggt
    10261 tttttgtaac cggagataaa tggaaaaact cctatgcact aataccttag aggatggggc
    10321 cgataggctt ttcttgacta atcaaataat acattccgag aatgctatat tattacatac
    10381 atttatagat ttaagggtaa ttatttgctc taagcagttt ggagaaggta aggcaataaa
    10441 aaagtaacac taggaggtag gtttggtttt cggaaaaaaa gagattgttg gactcgatat
    10501 cggttctagt gcgattaagc ttgcagagct aagggaggcc cgaaacgggt atcaacttaa
    10561 aaacatagga gaaagcctgc tgcctcctga ggctatagtt aacaaggtta taatcaattc
    10621 aggcgctgtg gtagaaacga tttcaacact tgtgaaggat cttggagtta aaacaaagga
    10681 cgttgcaatc tcgatttctg gacactccgt tattattaaa aaggtgagta ttcctcaaat
    10741 gagtgaaaag gagttgcgtg aggctattcc ttgggagctt gagcagaata tacctcatag
    10801 tattgataat gttaattatg atttccagat tcttcccgga gaaagtcaag aaggtaatat
    10861 ggatgtatta attgtagctg ctaaaaagga tataacagag gattatatag ccgttacgaa
    10921 cgaagctggg ctcaatcctg ttgtggttga tgtggatgtc tttgcccttg agaatatgtt
    10981 tgaaattaac taccccgtaa ctgatgaagt taccgcgtta attaacattg gagcttccgt
    11041 gacaaatatc aatattctaa agggcggggt ttctatattc gctagggata ttacagcggg
    11101 agggaggcaa tttacggagt ggatacaaaa agagtttaac attggctacg atgaggctga
    11161 gaaaatgaag tattctcttg ggctcaccgg agtttctaag gaacttgata ggataacgcg
    11221 tgattttact gaattaatct gtggagagat agaaagaact attgactttt tcgctacatc
    11281 actaaaagaa ccggttcgga agatttttct tggaggcgga tcttcgaagg ttccttacat
    11341 aaaagagcag cttcaagaaa gaataggttc ctcagtggag atggtaaatc cttttaaaaa
    11401 tatactccac gatgtgaggg atttcgaccc tgaatatatt cttgatatat caccgaagat
    11461 gggtgttgct atagggcttg cgataaggag aaaaggggat aaatgataaa aataaacctt
    11521 attcccgtaa aagaaggaaa agaaataaag ggtcttagag acctgattat aggtgttatt
    11581 gcgataatag ctgtatttat cttgcttgta gccttacaat tatggcggat taaacaaata
    11641 aaggatgtaa acattaagat tgctgacgta aaagcacgta ttttgaaact tgaggatgtg
    11701 aagaaaaagg ttgaagaatt taaagccaag aataaagaat tacaggagaa gatcgaggtt
    11761 ataaaagttc ttgaggaaaa ccgcaccggt cctctttacg ttatggaggc tctagggaag
    11821 gctattccta atagggcctg gatcgataaa ttatcggaga aaggttcgtc agcgaagatg
    11881 gagggttttg catgggatga gcttacagtt tcagatttta tgaaaaggct tcaatccttt
    11941 ccctatttcc aaaatgttga gctaaaggtt atcaatacaa aagaaataca gcaactacct
    12001 ttgaaggctt ttgtaataga gtccagattg aattattcag gcaaggtgaa gccggaggaa
    12061 aaacctcaag aaatacctga ggtaaagaag ccaagtcagc aaaaggctca gaggtgaaaa
    12121 tggctattat tgaaaatata agcgatgccc taaatcgtca gcgactctac gttaggcttg
    12181 gtattctaat tggcataata gtgattatgt gtggggtgtt ttggtatttc ttttggtctc
    12241 caaagacaga ggagctgaaa agcgcgagaa acgaacttca acggaatgaa gctaaactaa
    12301 gggagtacga agctatagca aaagagcttc caaagtttga gcaggagttt aagaggctaa
    12361 atagggaatt tgaagaagct tcccgtaagc ttcccaagga gaaagaaatt cctactttga
    12421 tcgatagtat atatgccgct gtatcagctt caggtctaga gtctaatacc ttcgccccaa
    12481 agcccgaggt gaaaaaaact atatacgctg agattccaat agaaatgaag gtctacacct
    12541 cgtattatga tcttgctaag ttctttaaca gggtctctaa gctccctaga attgtaaata
    12601 ttagggacct caatctcagt caggataaaa agaaaagcac aggccagaaa atagttctta
    12661 atgccgcctt tacagccgtg acgtttaggc ttctcccacc tcagcccgtc caaccggata
    12721 caaaaacgga tacaaaaaga aaaaaagcaa ccaagaaaac ggagcctaaa gaatgaacgg
    12781 aggtgcacaa ggcatcagga agggtttaat cttgttctta ttgataggcc tatgggtttt
    12841 tgtttctcat gctcaagaag atcaaaagtc ggcaaccgat tttaaaacaa tacaagaagg
    12901 ctctacacaa aagccatcag taaatcgtga tccatttaga cccttcatca agattgttga
    12961 aaaaaaggag gtggagaccg aggttggtaa atcggttcct cccattaaga ggtatccgct
    13021 tgaacaattc agattggttg gtattgtttg ggtaggggaa caaccaaagg taatggttgt
    13081 tgaccctgag aaaaacactt atgttctagg ggtaggagat gaaataggaa gcaggcaggg
    13141 aaagattatt gaggtaagag aaagtgggat catggttgaa gaaaaaaatt acttcgaaga
    13201 tgtgttcggg caaaaaaagg tcgagtcagt aaaatcagtc cttgcgttta aaggagaatg
    13261 agcaatagca ggagtgaagt ttaactcaag gaggatggtt ggatgaaact tatcctgaaa
    13321 gcagtttttg ctgggttctt tctttttgtt ttagtttcct ttttaaattc cgaatctagt
    13381 ggattagtta ctcaagcgaa cgataataag gatacccatg gtgttatcgc agaatttaat
    13441 aatgaaactg taaaagaaag tcctttactt cctaaaacaa ataagtcatt tgcgtctcag
    13501 tcttcagttg tgaattctac taacaaacgg catattgcta caggctcaaa taaggttgaa
    13561 agcattggtt ttggggttca gggtttgatt ggaatagtta caataaaaac aactgttcca
    13621 gtaaagtttg aagagcttcc taaaggggaa aagaggctta atttaaagct tttagatgtt
    13681 tttcttcctg aagaatttca ggtttcgcga gacgtaaatg agtttggtag cccagttagc
    13741 ttcataagta cctttaaaga ccttgctggt gaaggggatg taatagtcat tgtggaactt
    13801 aaggaggatg caaggactga gcttaatcag caggataatg tactcaccct aagatttaca
    13861 aaacagagtg gagaagaaac gataaaggta tcagagaaaa ctcaggtgga agaatctcag
    13921 cctacttcaa atcaatctgc tcctatagaa ggtgttgctg ctgattcaaa gcaagcggaa
    13981 cccgctgaaa agattgagga atcaccacct actactataa aagcgccaga aacccaccct
    14041 acaattcaaa cctcttcaag taaagaggct gtcaatcccg taataaagca agaagaagca
    14101 acaaaacagg ttgaggaatc gaaggcacct caaaaaaccc aaatagcgga atctcaacct
    14161 tcaactcaat ctaattcttc aaaagagaca gtggatacca gctacaaaga ggaaaatact
    14221 ccaaaggtga ttgagggaga tgtattaccc actacaacgc tgatggtaga atctcagccg
    14281 tccactcagt catttccatc tggagcgccc ggcagcgctt ccttgaacac tgtagagagc
    14341 ataggttttg aggtaaaaga gggggtagga atagtagcta taaaaacatc agctcctgtc
    14401 acatatgagg agcttcctaa agaagaaaaa aagcttaata taaatcttca agatgttttc
    14461 cttcctgaag aatttcaggt cagtagggac gttagtgatt ttgatagccc cgtaaggttc
    14521 atatcttcct ttagagaccc agttatagca aggagggtta taatcgtttt ggaactcaaa
    14581 gaagatgcac ctgttgtggt taaacaggag ggaaatttta tcactgtcaa cattggaaca
    14641 cccaaaggag aggaggagat aaccgatggg ttacagccgg tgccagaggg tccactctat
    14701 gcatttgatt ttcgtatcgc aactacgcca ggagcaaaga aggtctatcg aggtcagctg
    14761 gtttcctttg atttcaagga tgctgatgtg agagatgtgc ttaaaatcct tgcggacata
    14821 agcggtttta actttattgt ttcaagggat gtaaaaggaa ctgtcacttt aaaactttcg
    14881 aacgtaccat gggacttggc tcttgacata gtgcttgagg acgctactct tggcgcagtc
    14941 gttgaaggaa atgtaattaa ggtcgcccca cttaagacaa ttcaagctaa gcagatcgca
    15001 atcaggtcgg ctgctcaatc aaaagaacag cttgagcctc ttattacaaa acaaattttt
    15061 ataaactatg ctactgctga tgaactcaca ccccttgctg acccgcttct tagccaaaga
    15121 ggggatatta aggttgatac acgaacaaat agtatattgg ttacagatac agcaagtagg
    15181 gtaaggcaaa ttgaggagct cgtaaaaagc cttgatacaa ggactcctca ggtgttgata
    15241 gaatcacgca tcgttcaagc aacgctcgac ttcacccgtg agcttggtgt tcagtgggga
    15301 gtagattata aagcgtctgc tgctacagga aatccaacag gtgcaacctt tccttccagt
    15361 attaatgcag gcggtactaa tataggggct ccttttggaa ctcagggaaa taactttatc
    15421 gtagacttgc ctgccgctgt tggtactggg tcgggtggag cgataggaat agttcttgga
    15481 agtctttctg gagcttttga tctggatata aggctctcgg cacttgaaaa tagaggagat
    15541 ggtagggttc tttcttcacc aagggtattg acgctaaaca ataatccagc aaggatagag
    15601 caaggcgttt ctattccgtt cctcagtgtt tctgcagcag ggacccagac ccagtttgta
    15661 gatgctaacc tcacactcga tgttacccct caggtaacaa atgacaacag aatacttatg
    15721 gatataaagg ttacggataa ccgtcctgac cctacgctta cgggagcgaa tggacagcct
    15781 tctataagaa gaaatgaggc agaaacccag actatagcat cagacggtga gactattgtg
    15841 attggaggga tttatacaag agaaacatcg gaatctcttc gtggaatccc atggttcaac
    15901 aggattcctc ttcttggatg gctatttaga accgaacgga atatagactc aagaagggaa
    15961 cttataatct ttgtaacccc aaggatagtt aagtaaagga ggaataacaa aatggaaaaa
    16021 cccgagattt tgggtattgc tttattccct gcactcgtga tttcgacact tataatagcg
    16081 ctaagctgtg gtccaaaccc cccttttgca cctcagggct caactataac gattctaaat
    16141 cctcttgatg atattaccat accaccggag tcccttcagc cgacgttcag gatacaggca
    16201 tttgttactg accctgatgg aaacgcgtta aacagtgtta gggtaagatt ttccttatcg
    16261 tttgcagacc aaaattctct tgtagtagac acagatgggg atggagaaag cgattctcgc
    16321 gctcttcagc ttgttgacaa tagtaactgt aatccgaatc cttgtgatct tacacctata
    16381 tcacagtggt ttcagcaagg ggcgtttgtt gattcccctt atactgaact cagtaatgac
    16441 cgcgggattg ctgaaattat aatacttatt cccggccccg atgttgttat agtagatccg
    16501 gctgtttttg aagcctcgct tgataatggc tctcctcctg ataattttca gtttagcgta
    16561 aatgcagggg cacagtaaaa ccaaaaagac caatctcttt ttaatggagg aataattagt
    16621 gaaaagactg ataaaaacct cagatagcaa taataagcta aggcagtttt tatcgatttt
    16681 aactgtgttt ttggtgtttg ttgggcttaa ctgcgttaat ggcttagatc aaggctcttc
    16741 cgatgatcca gctcatactg gaataatttt tgttaacggt gtaccagtcg taatttttgg
    16801 aaattctatt gatcagagtc tcatagaggt tactacaggg gttttcccta atggctcgac
    16861 aatagaattt gagattacat tgtctgacct tatagaattg ctttcaggat gtgtatttgg
    16921 cggtgacgta attattgtag atggtcaggc ggttgctaat taccttgccg gtatacttat
    16981 tggaacacca gatcaactta gttcaggtga cccaccagta gcagcgttaa atgtagcggt
    17041 caaaataact actccggatg gagatagcga gtctgatttt gttacaatgt ttctaaatgc
    17101 cgtaggtatg ataccccctg aggatatgga tgttactacc tctcctgatg atgggataac
    17161 actaccagtc attgtcacat tggtatttca aactatagga attccgcctg gaacgaatgt
    17221 gagcttttcg ctcagtaacc ctgccttagg aatgcttaat cctctagtaa cgccagtgag
    17281 tggagcagaa gataatggcg aagcggaaac actgtatact actgtaaacg gttctagtgg
    17341 aatacagact ataacggcaa ctatcattct tcccaatcca tttgattttg acccatcttg
    17401 tccaaacgtc cctgtgggac aacgtacaat tcaggtatcc gttaaaatta atcaaatagc
    17461 gcccgttcct actccaactc cttcaccttc gcccacacca ggtcctacac ctactccgag
    17521 tcctacacct actccgggtc caactcctac tccatctcca acaccgggtc caacagtaac
    17581 acttgtatgc ccagatcaga tagtcgaggg tgaccaagcc caatgtacat gctcaacaaa
    17641 tcagggtcag ggggtacaat tatgtttcgt tgttagcgat ccccctctta cccttatttc
    17701 gcctactatg cctgattgtg atactacaga tggagcaggt gaagctttcg tagtcgtttt
    17761 ggcccaagat gttatgggag cggaaattgg tcaagtcgag tgttgtttat cacttggtga
    17821 tgatatgtgt gatcctggcg acttaaatga tgttgaacct ataacagttg tgcctgtacc
    17881 tacgcctaca ccaacaccat cgccaacgcc tggtccaaca ccaaccccaa ctccttctcc
    17941 aaccccaact ccagggccta tcatgatcac cgctggggac ctaaccccag cggcaggtgg
    18001 acaaactgtt attgaggcat tctcaaatca aaatgtagat gatct
//
LOCUS       DATDRL010000209         3225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1889997, whole genome shotgun sequence.
ACCESSION   DATDRL010000209 DATDRL010000000
VERSION     DATDRL010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889997"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..769)
                     /locus_tag="VI935_07160"
     CDS             complement(188..769)
                     /locus_tag="VI935_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25415.1"
                     /translation="MRHIPLVSLIVFIIFLSACGEGLLPTSSGNVDTMITEEDAIAQD
                     YNFRTPGYDMQVAIRDDVDGNINNILELLDERAEVFLECQFMMDPVIGFDDFKIPSGG
                     TVFPLSMLRVFVVPFNFECDAVDKSICAGIYYFGSDLIIIAEEGLGRCGKLPLWKHEL
                     GHRYGMAADHNNQDEFEPCIDPPDCDIIDIPDI"
     gene            1085..2089
                     /locus_tag="VI935_07165"
     CDS             1085..2089
                     /locus_tag="VI935_07165"
                     /inference="COORDINATES: protein motif:HMM:NF027495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HLE25416.1"
                     /translation="MKKIINKWVHIPGLHCGSVALRDVVTYYGYNLSEAMCFGLGGGL
                     GFFYIKGENMSPSRIIHLRGPGMEPNFFSLIDKPTSWKYEQDEDKAFEIAKEWINEDV
                     PILVQTDIFYLDYYKSSTHFPGHVVSVWGYDDETEIVFLADTGFRGLQPITYENFKKG
                     RTSKAMPFPLDNNWFEVNLKKAIRPLSEVIPEAIRRNAIFMTQGIKSLRGESGVKMLK
                     RWAEDLPIWADASDWSWCVRFAYQVIEKRGTGGGGFRWLYRDFLREAEEIIPALKELK
                     LSEKMDLIGNKWSAMSTLLKEISESEKNGNLFIKASENAKELGDLEEEFYNTVLDKIL
                     "
     gene            2225..2905
                     /locus_tag="VI935_07170"
     CDS             2225..2905
                     /locus_tag="VI935_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HLE25417.1"
                     /translation="MKLLLFDIDGTLILTGGAATRAVNRALNKIYGIDKAMDGIRPEG
                     KTDPAILREIFCKRIGRDLLTDELNIVFKEYVLFLREEVGNSHGYTIMPGIKNLIPAL
                     YKRKDVLLGIATGNIEEGAHIKLERAGLNSYFKFGGFGSDSEDREELIRIAIERGKKL
                     LNQDKKVDEKVFVIGDTPLDIIHGRGAGARTVAVATGSYSVSDLEKYEPYFLFENFLD
                     FEGVLKIF"
BASE COUNT         1054 a          613 c          633 g          925 t
ORIGIN      
        1 ttccgagatc tagcgcccgc cttgcggcag cgatagactc tttatcttcc gtctcacccc
       61 aacttccgcc cattgcccaa catccaaacc ctatagccga gagctttaag tcagaatttc
      121 caagtcttcg atattccaaa cttttctcct tttgaattcc attagtatta aatcacaatc
      181 aactaattta aatatcagga atgtcaatta tgtcgcaatc aggtggatca atacaaggtt
      241 caaactcatc ttgattgtta tggtcagcag ccatgccgta gcggtgaccc agctcatgtt
      301 tccaaagggg taattttccg catctcccaa gaccctcctc tgcaattatt attaaatcag
      361 aaccaaagta atatatgcct gcacatatgc tcttatccac tgcgtcgcat tcaaaattaa
      421 acggtaccac aaagaccctt agcatcgaga gcggaaacac tgtacctcca ctcggaatct
      481 taaaatcatc aaacccaata accggatcca tcatgaattg acattctaaa aaaacttctg
      541 ccctttcatc gagtaattcc agtatgttat tgatatttcc atccacgtca tctctaattg
      601 caacctgcat atcatatccc ggagttctga aattgtagtc ctgagcaatg gcatcttcct
      661 ccgtaatcat tgtatccaca tttcctgacg aagtaggtag gaggccttcg ccacaagctg
      721 ataagaatat gatgaaaaca atcaaactta ctaaaggtat atgtctcatc ttttaattta
      781 ctagttcgta ttaatctcga tcagtttata tgcctactaa ttttgtattc actagttaaa
      841 gttaaagaat tttacataat aaattctcga ttacaacact aaaaaaccga gtcatatact
      901 ctttttttca attgaccgaa tataaatcga agaaacaaca cactaaagag atgtaaataa
      961 tctattatgg catgagatga atatataaaa attgaacagt gttcgatact ccttcaatga
     1021 acaataaaag taatagtata gttaaatcta attccagttg ctaagctatg gtaaacttac
     1081 cttgatgaaa aaaataatta ataaatgggt tcatatacct ggcctacact gcggttctgt
     1141 agcacttaga gatgtagtta catattatgg ttacaatctc tcagaagcaa tgtgctttgg
     1201 tcttggcggg gggctaggtt ttttctatat aaaaggtgaa aacatgagcc cttcaaggat
     1261 aatacacctt cggggtcccg ggatggaacc aaattttttt agtttaattg ataaacccac
     1321 atcatggaaa tatgaacagg atgaagataa agcttttgag atcgcaaagg aatggataaa
     1381 tgaagacgtt ccaattttgg ttcagactga cattttctac ttagactact acaaatcctc
     1441 gacgcacttt ccaggacatg ttgtatcagt atggggttac gatgatgaaa cggaaattgt
     1501 ttttcttgcg gacactggtt ttagaggact gcaacccatt acatatgaga actttaaaaa
     1561 gggaagaacc tctaaagcca tgccttttcc tctagacaac aactggtttg aagtaaacct
     1621 taaaaaggct atccgaccat tatctgaagt tattcctgaa gctattcgaa gaaacgctat
     1681 attcatgact caaggtataa aaagccttag aggtgagtct ggagtaaaga tgctaaaacg
     1741 ctgggcagaa gatctcccta tctgggcaga cgcatctgac tggagttggt gtgtcaggtt
     1801 tgcataccag gtgatagaaa agagagggac cggcggaggc ggttttaggt ggctttacag
     1861 ggattttcta agagaagctg aggaaataat cccagcttta aaagaactta agctctctga
     1921 aaagatggac ttaattggaa ataaatggag cgcaatgtca acgctactta aagagataag
     1981 cgagagtgaa aaaaacggca atctctttat taaggcttca gaaaacgcaa aagagctagg
     2041 agatcttgag gaagaatttt ataacactgt gcttgataaa attctataaa taaaacataa
     2101 ctatatagcc acaaagacgc taagaaaagt tcaggggtta aaagttcaag ggtttaacgt
     2161 ttaactcttg aactgttaaa ccttaaactt cgtgctttgg tgccttagtg gccgagtggt
     2221 tacaatgaaa cttcttcttt ttgacattga cggaacatta attctcactg gaggtgcagc
     2281 aacaagggca gtgaaccgtg ccttaaataa aatctatgga attgataagg cgatggatgg
     2341 gatcagaccc gagggaaaaa ctgaccctgc aatccttaga gaaatcttct gcaaaagaat
     2401 aggcagagat cttttaaccg acgagttaaa tatcgttttt aaagagtatg ttctgtttct
     2461 tagagaggag gtgggaaatt cccacggtta tacaattatg cccgggatca aaaatctgat
     2521 cccagcccta tataaaagaa aagacgtcct tcttggcatt gctactggaa atattgagga
     2581 gggtgcccac ataaaacttg aacgtgcagg tcttaattct tatttcaaat ttggtgggtt
     2641 tggctcagat tccgaagaca gagaggaatt aattcgaatt gctattgaaa gagggaaaaa
     2701 gcttcttaac caagataaaa aggtagatga aaaggtcttt gtaatcggag atacaccgct
     2761 agatattatt catggcagag gagcaggggc aaggacagtt gctgttgcta caggatcata
     2821 ttcggtaagt gatttggaaa aatatgaacc atattttcta tttgaaaatt tcttggattt
     2881 tgagggtgtt ttaaagatat tttgactcga cccgcgaacc gacacaaata aacacgaaga
     2941 aaaagaaaag gtggtgagct tcctagaact gcttttgctg ttagaggttt aatagctgaa
     3001 atcaggagga gtaggggcac gttgcaacgt gctcctactt taattcgcgt aaatccgtgt
     3061 taattcgtgg ctaaatagtt ttaaaccacc cgtcttaaaa cagaactcaa cctttcataa
     3121 tcatccttaa aaatccttgc aaggctcttt cccattctga ctatataatc cttatcattc
     3181 tttctcacat cttgagcgta acgaagtgat gagataagaa gctcg
//
LOCUS       DATDRL010000210         4267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2753334, whole genome shotgun sequence.
ACCESSION   DATDRL010000210 DATDRL010000000
VERSION     DATDRL010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2753334"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VI935_07175"
     CDS             <1..481
                     /locus_tag="VI935_07175"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HLE25418.1"
                     /translation="ERSFFIARFATDSQCLDKYVYNRLPKKRRKDFFKDLAHLVAHLH
                     ESSFFHKNMNGENILITEKNEGCDIYLVDLDSVKYKGLKTKDRVENLAQLNCTFLDMR
                     KVSIRDRLYFLREYIINSQSLNLNNVLQKKFIDAIIKRSLKLAEKPGRRFLLNSLRI"
     gene            910..2427
                     /locus_tag="VI935_07180"
     CDS             910..2427
                     /locus_tag="VI935_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HLE25419.1"
                     /translation="MNLYRRIGQAVFSNTSIAPLAALFSFVGSVFVARYLSLETYGIY
                     VIVLAIKSTSLLYSDLGMSAGASRFFPELEKKEGYTGVLRGLKGVFVTRFTLAAFSML
                     IIIAFREKLSSIYGLSDQHSYLFVLVGILIFTDSCTLTIQSYLRANLEQKAINLVDLA
                     FSIIKSSLIILSVILGYGLKGLLIALVVSSALNAFFFSVLLYSRLRYVEKKLPQKSDK
                     AENLFKRFIKLSLSVFFIKQTSYFTSLSFIILVMGLYFNKQEIAFFSLAAGFANKAYN
                     MTLMPTKGVLLPSFSFSILTGDRSKIQLSFTYSLKFVSFLIFPVSMFLICLCPILIPM
                     IYTEKFLPSVILLRIILFFSLFASLTYSISSELLIAGEHLRVYVFVMLLSILSVPIFF
                     LVSQVGLVAIATFYGFVQLSVVVALTILVIKLFNLKFPTQHFIKMASATITVGLVLLG
                     IKYVLDDNIIIIILSFISLLAFLPLLRLLNIFSDQDKDVIRKLGIPFNEHVVKYF"
     gene            2871..3908
                     /locus_tag="VI935_07185"
     CDS             2871..3908
                     /locus_tag="VI935_07185"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HLE25420.1"
                     /translation="MPGYKHLVSSQAFNQYFSLEARAILENIRLMACISTVLALFAFI
                     RTKVDFHRIIKIFVITGTLNAIYGIYQIISLWKTPWLPMLPGAYIKLELLRASGTLWE
                     YSHYGSFMLIVTLLNLYIIGISKKILWKITAIFNITALFLSFSATAWIACTASLSVLI
                     FAQTYKPAVRKIGNSFGMLIILSVAVFLLLNAFDPIVEKVIWKITKDSPEKFERLEST
                     KNVFKIFLENPLGIGQGMYVFLFGGALAYLHIPVELGVFGIFLWGSILIVIFLAILKL
                     GHNSSTAGYTKRFFPYALAMFIGTNIFMLNYVTLNHEWVWFELALPVVALKLALKKDY
                     YNIEARPVSGL"
     gene            3916..>4267
                     /locus_tag="VI935_07190"
     CDS             3916..>4267
                     /locus_tag="VI935_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25421.1"
                     /translation="MIYLKKNIRLYASFLALLFLTTDTDITLGNIQTHIQKKKLIEFG
                     WDIPDTEFVKNNISEMEKKPFNGVVLHVKYNNQDKYLESIAFSNIYLQYEGVSKAIED
                     LKATNFEKFTDNFLR"
BASE COUNT         1327 a          709 c          745 g         1486 t
ORIGIN      
        1 agaaagaagt tttttcattg caagatttgc tacagattct caatgcttgg ataaatatgt
       61 ttacaatcgt ctcccaaaaa agaggcgtaa agattttttt aaagatttag cacacttagt
      121 agcacactta catgaaagca gcttcttcca taaaaatatg aacggagaga atatactaat
      181 aacagaaaaa aacgaaggat gtgatatata tcttgtagac ctagactcag ttaaatacaa
      241 gggtcttaag acaaaggacc gggttgaaaa cttagctcag cttaactgta cctttttgga
      301 catgagaaaa gtaagcatta gagataggct ctattttcta agggaatata taattaactc
      361 acagagccta aatcttaata atgtcttgca aaagaaattt attgacgcca ttataaagag
      421 gtctttgaaa ctagcggaaa aacccggaag gcgttttctt ttgaatagtc ttaggattta
      481 aaggtacctg aaatctcaat ctatacgctg gcacacctaa acagaagaat tccaaaagcc
      541 ttcctttcca ataaggacta attctagtta actgccaaat agttctttcg aatttcctag
      601 ttaaaccgca actagcgcaa cgtccttgcc cagtagaaag tctccaaaat ttcttgctac
      661 tgcagcgatg acaaggtcta atataaagaa tttaccgtat ttgtaggtat attgttaatg
      721 ttctaagtgc aactttgata accaattacc tataattaaa tatgggagta tctgattttc
      781 tttgataaaa taataataga atcatgtatg agagcctaag gagattaaaa tttttatgat
      841 actagggaga agattataaa tgagaattat ttatatctct ggagcttgta ttccgagcga
      901 ggaggcgcta tgaatttata tagaagaata ggacaagctg tattctctaa tacgtctata
      961 gcaccccttg ctgccttgtt ttcctttgtt ggctccgttt ttgttgctcg atacctctct
     1021 ctcgaaacgt atgggatcta tgtaatcgtg ctggctataa aatcaacttc attactatat
     1081 tcggatttag gtatgtctgc tggtgcatct agatttttcc cagaactaga aaaaaaggaa
     1141 ggatacacag gcgttctaag ggggttaaag ggtgtgttcg ttactaggtt tactctagct
     1201 gctttctcca tgctaataat aattgctttt cgagagaaat tatcatcaat ctatggttta
     1261 tcagatcaac attcttattt atttgtactt gttggaatat taatttttac agactcatgc
     1321 acactaacaa tccagagtta ccttcgagca aacttggagc aaaaagccat aaaccttgta
     1381 gacctagctt tttctataat taagtctagc cttataatat tatctgttat tctcggatat
     1441 ggattaaaag gtttattgat agcactagtt gtctcgtctg ctctaaatgc tttttttttc
     1501 tctgtactcc tttattcaag gttaagatat gttgaaaaaa aattaccaca aaaatcagat
     1561 aaggcagaga atctatttaa aagattcatt aagctatctt tatctgtctt ctttataaag
     1621 caaacgtctt atttcaccag tttgtcgttt ataatattag ttatgggatt gtattttaat
     1681 aagcaagaaa ttgcattctt ttcacttgca gcgggatttg ctaataaagc ttataacatg
     1741 actctaatgc ctactaaagg ggtactacta ccttcgttct cgttttctat tttaacaggg
     1801 gatagaagta aaattcaatt atcttttact tactccctta agtttgtatc tttcttaata
     1861 ttccctgtta gtatgttttt gatctgttta tgtcctatat tgattccgat gatttacaca
     1921 gagaaatttc tgccaagtgt catacttcta agaataattt tgttctttag tctctttgca
     1981 tctcttactt acagcatatc gagtgagctt ctaatagctg gagagcattt gagggtttat
     2041 gtttttgtaa tgttgctttc aattctctct gtaccaattt tctttttagt gtctcaagtc
     2101 ggactggtag ctatagccac tttttatgga ttcgttcaac tcagtgtagt tgtagcctta
     2161 actatactcg taattaaact tttcaacctt aagtttccga cccaacactt tataaagatg
     2221 gcaagtgcaa caataactgt aggattagta ctcttgggaa taaaatatgt attagatgat
     2281 aatattataa tcattatact gagttttatc tcgctacttg cctttttacc attactaagg
     2341 ctcttaaata ttttcagtga ccaagataaa gacgttataa gaaaattagg tattcctttt
     2401 aatgagcatg tggtgaagta cttttaattc ggctaaagaa cgcaatggat ggagtacgct
     2461 tcttaaggta ttatagcaat aattatctga attaaggcta gcagtgtaac atagtagctt
     2521 tgcttttttt cacctgaggt tatttagagg tgtgatatac gagatagaag ctcccccagt
     2581 acagtgtata aagattatca atttactccg cgacgtatag ttcaaaatct tttaagtttc
     2641 tacccagtct ctttgtatta atggtttttt gttttccatt cactcaagct ttaactataa
     2701 aattaggtgc acgctttatt attagtgaaa ttattgcttt gtttttagct ccttatgtct
     2761 tcttcctcgg taaagggaag cggtctacta cagagaggct gcttgtagtc cttttagtag
     2821 tttttttagt gtatagcatc tttatctcaa tcgtcggtct catctggata atgcctggat
     2881 ataaacactt agtgagtagc caagcattta atcaatattt ttctttagaa gcaagagcta
     2941 tcttagaaaa tatccggttg atggcttgta ttagtacggt tttagcttta tttgccttta
     3001 tccggacaaa agtggatttt catagaataa ttaagatatt cgttattacg ggtactttaa
     3061 atgctatcta cggcatatat caaattatta gtctttggaa aactccttgg ctacctatgt
     3121 taccgggagc ctatataaaa ttagaattat taagagcctc aggaaccctc tgggaatatt
     3181 ctcattacgg cagttttatg cttatagtga cacttctaaa tctgtatata attggtattt
     3241 caaagaagat actatggaag ataacagcga tttttaatat cacagcttta ttcttgtcct
     3301 tctctgcaac cgcttggata gcttgcaccg cgagtttaag tgtgcttata tttgcccaaa
     3361 cctacaaacc agcagtgaga aaaattggga attcatttgg aatgcttata atattaagtg
     3421 tagctgtttt tctactatta aatgcttttg atcctatagt agaaaaagtt atttggaaaa
     3481 taactaagga ctcacctgaa aagtttgaac gtttagaatc aacaaaaaac gtgttcaaga
     3541 ttttcttgga aaatccactc ggcataggac aaggtatgta cgtatttctt tttggtgggg
     3601 cacttgcata cctgcacatt cccgttgagc ttggtgtctt tggtatcttc ttatggggat
     3661 cgattttaat cgttatattt ttggccatct taaagctagg tcacaattct tcaacggctg
     3721 ggtatactaa gagattcttc ccttacgcac tcgcaatgtt tataggaaca aatatattta
     3781 tgcttaatta tgtaactctt aatcatgaat gggtctggtt tgaactcgct cttccggttg
     3841 tagccctgaa attagcttta aaaaaggatt actataatat agaagcaaga cccgtttcag
     3901 gtctgtaaaa agaggatgat ttatttaaaa aaaaatatta ggctttatgc ttcttttctt
     3961 gctttattat tcctaacgac agacaccgat ataaccctag gaaatataca aacccacatt
     4021 caaaagaaga aactaatcga atttgggtgg gatatacctg atacagaatt tgtaaagaac
     4081 aatatatcag agatggaaaa gaagcccttt aatggagttg ttctacatgt taaatacaac
     4141 aaccaggaca agtatctaga atcgatagcc ttctcaaata tatatctcca atatgaaggg
     4201 gttagcaaag caattgaaga cttaaaggca actaattttg agaagtttac agataacttc
     4261 cttagat
//
LOCUS       DATDRL010000211         9730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2785261, whole genome shotgun sequence.
ACCESSION   DATDRL010000211 DATDRL010000000
VERSION     DATDRL010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2785261"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VI935_07195"
     CDS             complement(<1..404)
                     /locus_tag="VI935_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25422.1"
                     /translation="MAQLHKKFTDSQIKELLERYVINEIERKYIQQVLGIKDRRFFIL
                     IKRYRENPKGSSIQYSRKAKTRGITPSIEENLIKELSIEKELIQDENVPLKSYNYSYI
                     KDLLEKKYNQTVSLPTIIDRAKKNDFYLKKRP"
     gene            601..873
                     /locus_tag="VI935_07200"
     CDS             601..873
                     /locus_tag="VI935_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HLE25423.1"
                     /translation="MQDKQKIIKRLKNIEGHIRGIQRMIEDDKYCIDVIKQALAVKSA
                     VDKVNALILESHLKSCVTTAIRSNKQVERERVIAELLDVFETSGKI"
     gene            932..3379
                     /locus_tag="VI935_07205"
     CDS             932..3379
                     /locus_tag="VI935_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HLE25424.1"
                     /translation="MKSIKIGSTQMVKDPVCGMNIDPESAAGSYEHEGRTYFFCNPHC
                     LQKFKAEPAKYLTGAPAIPHKESEPRSDAKDNVYICPMDPEVRQQGPGSCPKCGMALE
                     PISPVTPTAGIQYTCPMHPEIVRDEPGFCPICGMALEPITVTGEEVNEELVDMTRRFK
                     IGLALTIPLVFLAMSDLIPGQPVQKVISTHILKFIQLALAAPAVLWGGWPFFKRGWAS
                     IINRSLNMFTLIAIGTGVAFWYSVVATFFPNIFPDSFRSQGGEVGVYLEVASVIVLLV
                     LFGQVLELRARSQTSSAIKALLGLAPKTARVIRDDGTEEEIPLDNIKPGDRLRVRPGE
                     KVPVDGIVIEGESFVDESMITGEPIPVEKRKGDKVVGGTVNSTGGFVMQAERVGSETL
                     LAQIVRMVSEAQRSRAPIQRLADIVSSYFVPAVILIAVITFIVWSLIGPDPRLAHAIV
                     NAVAVLIIACPCALGLATPMSIMVGTGRGATAGVLIKNAEALEVMEKVDTLIVDKTGT
                     LTEGKPRLSSVVPLPGWNELELLRLAASIERSSEHPLAAAIVAGAQRKNLKLSEPDEF
                     ESITGKGVVGKVDGRLVALGNSKLLEQLNIEVGGMLERAEALRKEGETVMFVAVDGQL
                     AGLMGVSDPIKESTPEALRMLHEDGIRILMLTGDNRITAEAVARKLGIDEVQADVLPE
                     QKGEIVKRLQAEGRMVAMAGDGINDAPALAQAQVGIAMGTGTDVAIESAGVTLVKGDL
                     RGIARARRLSRATMRNIRQNLFFAFIYNALGVPIAAGILYPAFGLLLSPIIASAAMSF
                     SSVSVIGNALRLRKAEL"
     gene            complement(3564..4214)
                     /locus_tag="VI935_07210"
     CDS             complement(3564..4214)
                     /locus_tag="VI935_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HLE25425.1"
                     /translation="MKKQNIVLISAICMVIVFVLGSYFYKNQQLKKLGFMAKENASTF
                     VREYSLTLGSDDAKVYIVEFFDPACETCRDFYPFVKNLMAANPGKMKLVVRYAPFHPG
                     SDYFVKILEAARKQGKYWETLEVMFNSQPYWASHHDPQPQLIWQFLPQAGLNMEQIRN
                     DMNDPEIAKHIEQDLADAKTLNVRKTPEFFVNGKPLPSFGYNQLRELVETEIQANY"
     gene            complement(4211..4663)
                     /locus_tag="VI935_07215"
     CDS             complement(4211..4663)
                     /locus_tag="VI935_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HLE25426.1"
                     /translation="MENTNMPRLDQTSNSNWTILFICWLIASISTLGSLFFSEIMKFP
                     PCVLCWYQRICLFPLVLILSMGLFPFDKSVVKFAPPLALAGWFIAFYHNLLYSGIIPE
                     NIQPCSQGVSCTEDYINLFGIFTIPMLSIMAFSIIGVLLFILKRRLSK"
     gene            complement(4551..5069)
                     /locus_tag="VI935_07220"
     CDS             complement(4551..5069)
                     /locus_tag="VI935_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25427.1"
                     /translation="MVSALKITCQALRLSQLITSRKTGQDCAVGTNFAALLCLIAGCV
                     HTWKLMPLDVFAPPILVLVKMLSNSMWNGRAFSGVPICRSVLARRSQRRYVSPIISDS
                     QTWAGTWLEILKESSSEKLRHVLHQPILQKFLNYGEYQYASFRSNIQLKLDYSFYMLA
                     YSQYLYTGESVF"
     gene            5883..5975
                     /locus_tag="VI935_07225"
                     /pseudo
     CDS             5883..5975
                     /locus_tag="VI935_07225"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
     gene            6049..6261
                     /locus_tag="VI935_07230"
     CDS             6049..6261
                     /locus_tag="VI935_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011319271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HLE25428.1"
                     /translation="MKSKIISSSPDVMGGTPVFTGTRVPVQTLLDYLEGGESIDDFLE
                     GFPTVTREQVIAFLEEAKEQIVASVK"
     gene            6263..6583
                     /locus_tag="VI935_07235"
     CDS             6263..6583
                     /locus_tag="VI935_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015216317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HLE25429.1"
                     /translation="MKILLDECIDWRFAKELSGYFVETVPRMGWAGSKNGDLLMLAEK
                     QFDIFITVDRNLSFQQNLPQLNIAVLILHAPTNRLADLKQLVPNVLATLPTVKVGQIV
                     TISI"
     gene            complement(6658..6852)
                     /locus_tag="VI935_07240"
     CDS             complement(6658..6852)
                     /locus_tag="VI935_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002734063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25430.1"
                     /translation="MSTSVKHILKTFDLLSDKEKKEVASEIIRRSASFDLPPLSDEEL
                     VLSAEELFLELDRQEFKDGH"
     gene            7312..7506
                     /gene="rpmB"
                     /locus_tag="VI935_07245"
     CDS             7312..7506
                     /gene="rpmB"
                     /locus_tag="VI935_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014955590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HLE25431.1"
                     /translation="MAQVCVVCGKKPLYGHTVSHANNKTKRRWNINLRRVKARLDSGE
                     IRRIRVCTNCIKSGRVQKAA"
     gene            complement(7503..8015)
                     /gene="def"
                     /locus_tag="VI935_07250"
     CDS             complement(7503..8015)
                     /gene="def"
                     /locus_tag="VI935_07250"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HLE25432.1"
                     /translation="MAILKILTYPDPRLKKKSTPVEKIDKEIEKLLDDMAETMYKAPG
                     VGLAAPQVGINLRVIVIDISARQEDSPGLIELINPEIISSEGVQIEEEGCLSIPGFSS
                     EVKRKAKVKVQGVDRKGKLVEYEGNGLLARAFQHEIDHIDGILYIDRLSRLKRELLKK
                     KIEKSLAREN"
     gene            complement(8134..8421)
                     /locus_tag="VI935_07255"
     CDS             complement(8134..8421)
                     /locus_tag="VI935_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HLE25433.1"
                     /translation="MKRSELERELSAKFGLQPQQSEQIIDAVINHMTDVLQGGGRIEI
                     RGFGSFFTKVYKPYSGRNPRTGETVQVPSKKLPHFRPSKELRKKMNEVQSQ"
     gene            8578..9447
                     /locus_tag="VI935_07260"
     CDS             8578..9447
                     /locus_tag="VI935_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE25434.1"
                     /translation="MMGRLERILNKADNFKESERFEEALRELTKALKIAPHDPDVYLS
                     FALTYDAMEEFETAVAYFKKALELGPKDCYILTQFGITLGRMGRYIDALTVFEGALTI
                     EPGYVVAKWNLALTLRAIGCCEDALSGFMQCMTTDTDSDYIKSEVHYQIGLCYFDMGW
                     TPEALVELNKQVELFPDDIWAHLSIGNCYLDCGWVDESISKYREIISACPDFIPAYNS
                     LALSLAEKGWYDEALDVLRVALEYAPEDESIKDNIDYIQSLKDDENGFKGIITYCLIN
                     QILKRRHSLKNSR"
     gene            complement(9547..>9730)
                     /locus_tag="VI935_07265"
     CDS             complement(9547..>9730)
                     /locus_tag="VI935_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HLE25435.1"
                     /translation="NMVFWVLVIIGIVLLVIWVVRRAGGERSKAANSALEILKKRYAR
                     GEITKEEYEEKKRDIS"
BASE COUNT         2748 a         2031 c         2165 g         2786 t
ORIGIN      
        1 ttaggcctct tttttaaata aaagtcgttc ttcttagctc tatcaataat ggtaggtaag
       61 gagacggtct gattatattt tttctctaag aggtctttta tgtagctgta gttataggat
      121 ttaagaggca cgttttcatc ctgtattaat tccttttcaa tagataattc ttttataaga
      181 ttttcttcta tactcggggt tatacctctg gttttggctt tcctactgta ttgaatagaa
      241 gagcctttag gattttctct gtatcgttta ataagaatga agaatcttct gtctttgata
      301 ccaagaacct gttgtatata ttttctttca atctcgttta tcacataccg ttcaagaagt
      361 tctttaatct gactatctgt gaatttctta tgtagctgtg ccataatcta aaatcccctt
      421 tttgggtttt agattatgtc tatttaggtg tgcactttta aactttaaat cctacaaaaa
      481 ctatgcactt ttaattttta agtgacaaca tataaaatag acttgacaga aaggttgctc
      541 aacattattt tatatacccc accctagtgg gaaggggaat tataaaaagg aggtaacgat
      601 ttgcaagata aacagaagat aattaaaaga ttaaagaaca tagagggaca tattcggggc
      661 atccaaagaa tgatagaaga tgataaatac tgcattgatg ttataaaaca agcacttgct
      721 gtaaagagcg cagttgataa agtaaacgct cttatactcg aaagccacct caaaagctgt
      781 gtgacaacgg ctataagatc aaacaaacag gttgagagag agcgggtaat agcagaactt
      841 cttgatgtat ttgagacatc gggaaaaatt tgatttaaaa gtcttaaacc ttctggcttt
      901 ttaacctaag catatttagg agattatcaa gatgaagagt ataaaaattg gaagcactca
      961 gatggtgaaa gaccccgtct gtggtatgaa catagatccg gaatctgctg ctggctcgta
     1021 tgagcacgag ggaagaactt acttcttttg taatccccac tgcctgcaaa aatttaaggc
     1081 tgaacctgct aaatacctga ctggcgcacc ggctattcct cacaaagaat cagaaccacg
     1141 atcggacgct aaggacaacg tatacatctg ccctatggac cccgaggttc gacagcaggg
     1201 gccaggctcc tgcccaaaat gtggcatggc cctggagcca atatcaccag taaccccgac
     1261 agcgggaata caatacacct gtcccatgca cccggagatt gtgcgtgacg agccaggctt
     1321 ttgtcccatt tgcgggatgg ctttggagcc tataacggtt acaggtgaag aagtcaatga
     1381 agaactagtg gatatgacgc gcaggtttaa gattggcctt gcccttacaa ttccgcttgt
     1441 gtttcttgct atgtcggacc tgatcccagg ccagcctgtg cagaaagtta tctcaactca
     1501 cattctcaag tttattcaac tagcacttgc cgcccccgca gtgctatggg gagggtggcc
     1561 cttctttaaa cgcggttggg cttcaatcat caatcgtagt cttaacatgt tcacccttat
     1621 tgccattggc acaggagtgg cattctggta cagcgtggtt gccacctttt tcccgaatat
     1681 atttccagac tcgttccgta gtcagggtgg tgaggtaggt gtctatttgg aggtagcttc
     1741 agtaatcgta ctgcttgtgc tttttggcca ggttctcgaa ctgcgtgcac gaagccagac
     1801 tagcagtgcc attaaagcgc ttctgggact tgctccgaag actgccagag tgatacgcga
     1861 tgacgggaca gaggaagaaa tcccacttga taacatcaag cctggtgatc ggctccgagt
     1921 tcgccctgga gagaaggtac ctgttgatgg tatcgtcata gaaggtgaaa gctttgtaga
     1981 cgaatctatg attactggtg aacctatccc agtggaaaag aggaagggag ataaggtagt
     2041 tgggggtaca gttaacagca caggtggttt tgttatgcaa gccgagaggg ttggaagcga
     2101 aaccctttta gcccagatcg ttcgaatggt aagcgaggct cagcgaagcc gcgcccctat
     2161 acaacgtctt gccgatattg tctcttccta ttttgtccct gccgttatac taatcgctgt
     2221 gattacattc atcgtatgga gcctcattgg tccagaccca cgcctggctc atgccatagt
     2281 caatgccgta gctgttctca taattgcctg tccttgcgct ttagggcttg ctactccgat
     2341 gtctattatg gtcggaacag gtcgcggagc cacagcgggc gtgctcataa agaatgcgga
     2401 ggcccttgag gttatggaga aggttgacac ccttattgtg gataagaccg gtacgctcac
     2461 cgaaggcaag ccgcgcctta gttcagtagt gccgttgcca ggatggaatg aattggagct
     2521 attgcgcttg gctgcgagca tcgagagaag tagtgagcat cctctagcag cagcgatagt
     2581 agcaggtgcc cagaggaaaa acttaaagct ctccgagcct gacgagtttg agtcaatcac
     2641 gggaaaaggc gttgtcggaa aggtagatgg ccgtcttgtt gctctgggca acagtaaact
     2701 attagagcaa ctgaatatcg aagttggtgg tatgcttgaa cgagcggagg ctttgcgcaa
     2761 ggagggtgag acggtgatgt ttgtcgctgt tgatggccaa ctagcaggac ttatgggggt
     2821 atctgaccca atcaaggaat caactcctga agcgctccgg atgctgcacg aagacggcat
     2881 acgaatcctg atgcttacgg gggataatag aatcactgct gaggctgtgg ctcgaaagtt
     2941 aggaattgat gaggttcagg cggatgtgct gcctgagcag aagggcgaga tagtaaagcg
     3001 ccttcaggca gagggtcgta tggttgctat ggcaggcgat ggcattaacg atgccccggc
     3061 tcttgctcag gctcaggtag gcatagcaat gggaaccggt acagatgtag ctatagagag
     3121 cgcaggagta acccttgtga aaggagactt gcgtggtata gcgcgggccc gcaggctcag
     3181 tcgagctaca atgcgtaata tccgtcaaaa tctctttttt gcattcattt acaacgcact
     3241 cggagtacct attgccgcag gaatactcta tcccgctttc gggttactgc tcagtccaat
     3301 aatcgccagc gcagccatga gctttagctc tgtatcggtt ataggcaatg ctttgcggtt
     3361 acgcaaagcg gagctatagg ggtggaagta aaactgtaat taaaccacct acgtagaagt
     3421 cagtgaatcg aaccactgaa tgacatctaa atgcgaaaaa ctattctgca aacctcaaat
     3481 caataacctg ccgaaaaagt ggcctgtggt tcgatttcac tgcactgact ggttatgtgt
     3541 tctttgcttc tgataattta aatttaataa tttgcttgaa tttcagtttc tactaattct
     3601 cttaattggt tgtaaccaaa gcttggaagc ggcttcccat ttacaaaaaa ttcaggtgtt
     3661 ttcctgacat tgagagtttt agcatctgca aggtcctgct caatatgttt agcaatttcc
     3721 ggatcattca tatcatttct aatttgttcc atattaagtc ctgcctgtgg aagaaattgc
     3781 catattaatt gaggctgggg atcatgatgg cttgcccagt aaggttgact attaaacatg
     3841 acctccaaag tttcccaata ctttccttgt tttctcgccg cttcaagtat cttaacaaaa
     3901 taatctgagc cgggatgaaa aggagcgtat ctgacaacaa gtttcatttt acctgggttg
     3961 gcagccatca agtttttaac gaaaggataa aaatctctac aggtttcaca tgcagggtca
     4021 aaaaattcta cgatataaac ttttgcatca tcactgccaa gtgttagtga atattctcta
     4081 acaaacgttg aagcgttttc tttggccata aagcctagtt tttttaattg ctggttttta
     4141 tagaaataac tgccaagcac aaaaacaatc accatgcaaa tagctgaaat taaaactata
     4201 ttttgttttt tcatttagaa agcctcctct ttaatataaa taataatact cctatgattg
     4261 aaaatgccat tatcgacagc attggaattg tgaaaatacc aaacaagttg atgtagtcct
     4321 ctgtgcatga aacgccttga ctgcagggtt gtatgttttc aggaataatc ccagaataaa
     4381 gaaggttatg ataaaatgcg atgaaccatc ctgccaaagc taaaggaggg gcaaacttaa
     4441 caacgctttt atcaaaggga aagagtccca ttgataatat taacaccagg gggaataaac
     4501 aaattctttg ataccaacac aaaacacaag gaggaaattt cattatttcg ctaaaaaaca
     4561 gactccccag tgtagagata ctggctataa gccaacatat aaaaagaata gtccagtttg
     4621 agttggatgt ttgatctaaa cgaggcatat tggtattctc cataattgag aaacttttgt
     4681 aaaatgggtt gatgcaaaac gtggcgtagt ttttcggacg aactctcctt cagaatctca
     4741 agccatgtgc ctgcccatgt ttggctatcg ctaatgattg gtgacacata acgtcgttgt
     4801 gaacggcggg ccagcactga cctacaaatt ggcacgccgc tgaaggcccg tccgttccac
     4861 atagagttag aaagcatctt aaccaagacc aatatgggcg gggcaaagac gtccaatggc
     4921 ataagcttcc aggtatgcac gcaaccagcg atcaggcaca acaaggcagc gaaatttgta
     4981 cccaccgcgc agtcttgccc cgtctttctt gaggtgatga gctgtgaaag acgaagcgcc
     5041 tgacatgtga tctttaaggc gctgaccaag acccccgcgt gctttcggag tgcgaccaac
     5101 atgaaccact ttgcccttgg gatcgtaaat cacgtaaacg cctttcccac gtggtgcatt
     5161 aagcggttcg cgatgctgtg ggaatttctg caacggcgcg cggagaagcg tggcgaatag
     5221 tgctctcact gtgcggtagt tgcgcgtcac tgttccatgc tttctaacta ttgattatac
     5281 ggaattattt ccgcataatc accccaatat agggtgttat acggaaatcc ttgcaccttt
     5341 tttctttctt aggagtataa tttaaggtgt ctccctaagt caatggctac gggaaaaatt
     5401 atggaacaac gtcccaagaa attacccgat caggttcgtg agaccataag acgcaagcac
     5461 tattcctgta tgatatattt tatttatctt ggaaattatc aaaagtaggg tagctcacca
     5521 gtccctgcca tgagcagggt cgaagggttg ccggtcgagg agtaggcaaa tcgtctccta
     5581 attgtagggg caacccttgc ggttgcccaa aaagggcagg tgcaagtcct gcccctacat
     5641 ttatgcaaat gatttagcaa cgggcaagcc tgtcccgagc tccgtcgagg gaactcgttg
     5701 cctacccagt taagttattc cattatgaga ttaataattg attaaacata aatatatttc
     5761 atttcttaat ggtataagat tcgtgtgaaa ggaggagaat cattatggca aaagacccta
     5821 tatgtgggat ggatgttgat gaaaagaagg cagcggcaac atcctagcaa aagggaaaaa
     5881 cctattattt ttgtgctaaa ggttgcaaaa tcgcatttga caagaatccg gaggagtatt
     5941 tagggaagga gaaagaagag ggacacaagc actgattagg gatcgggatt tcggaaaaag
     6001 gtaactacca ttcttgattt ttcgaccggt tttacgaata ctgtaattat gaagagcaaa
     6061 atcatcagtt catctcctga cgtcatgggg ggaacccctg tgtttactgg aacgcgggtg
     6121 cccgtccaaa ctcttctcga ttatctagaa ggaggagagt ccatagatga ttttctggaa
     6181 ggcttcccca ccgtcacaag agagcaggtc attgcttttc tggaagaagc taaagaacaa
     6241 attgtcgcca gcgtgaagta agttgaaaat tcttctcgat gagtgtatag actggcggtt
     6301 tgccaaggaa ctgtcaggct atttcgtgga aactgtgcca cggatgggct gggcaggttc
     6361 aaaaaatggt gatctgttga tgctggcaga aaaacagttt gatatcttca ttacggtcga
     6421 tcgcaactta tcgttccaac agaacttacc ccaattaaac atcgctgtct taattttgca
     6481 cgccccaaca aatcggctcg cagatttaaa acaactcgtt cctaacgtcc ttgccacgct
     6541 tcccacagtc aaggtcgggc agatagtgac tataagcatc taacatcggt cttgggcgac
     6601 ggtgcgtttc gcttctctgc cagcatcact ttcataagca cttcgataat aatttatcta
     6661 atgaccatcc ttgaattctt gcctgtcgag ttctagaaag agttcctcag cactcaaaac
     6721 aagctcttca tccgagagcg gcggcaagtc gaaacttgct gagcgccgga tgatttcaga
     6781 agctacttcc tttttttcct tgtcagacag aaggtcaaaa gttttaagta tgtgttttac
     6841 cgatgtactc attttatcaa cccctaatca aaacctcgat tgggttagtc gaaattatca
     6901 acgatagcta atattatact ataaaattgc ctgattcatg aacacctcga ctggtgagat
     6961 ttcgcgacct agccgaaaat gaggtgttct acatcctcac aggtagaaga gccattcctt
     7021 cgatgacctc aggacaggct tggcacaata caaaaattat cctcttgtag gagcaccatc
     7081 ctggtgcgat tcatatcgcg gctagaagcc actcctacat agttacgtag gttatgtggg
     7141 cacgattcca tttatggagg gctaaagccc tccgctacat ttaattttcc acgcttgaaa
     7201 gttataccga actcacgtga gtttatagtg tgtattaaag ccttttagct tgaaataact
     7261 caccttactg ggtatattga attcacttat ttgattagga aggattctta aatggcacaa
     7321 gtttgtgtag tttgcgggaa aaaacccctt tacggacata ctgttagtca cgcaaataac
     7381 aagactaaaa ggcgatggaa cattaaccta agaagggtca aagcacgtct tgatagtgga
     7441 gagataagaa ggatcagggt ctgtacaaat tgtattaaat ctggacgtgt tcaaaaagct
     7501 gcttagtttt ctctagcaag agacttctcg attttcttct ttaatagttc cctttttaat
     7561 ctgcttagtc tatctatgta aagtattcca tctatatggt ctatctcatg ctggaaggca
     7621 cgggcaagaa gtccatttcc ttcgtattca acgagttttc cttttcgatc aacgccttga
     7681 accttgacct ttgcctttct tttaacctca ctagaaaatc ctggaatact taagcaacct
     7741 tcttcctcta tttgcacccc ctcggaagag ataatctccg ggtttataag ctcgattaga
     7801 ccgggtgagt cctcctgtct tgcagagatg tcaattacaa tcaccctcag atttatacct
     7861 acctgtggag cggctagtcc tactccaggg gctttgtaca tagtttcagc catatcatca
     7921 aggagttttt cgatctcttt gtctatcttt tctactggag tggatttttt tttaagccta
     7981 ggatcgggat aggttaggat ttttaagata gccatggcag gctaaaatcc tatccgaaag
     8041 aaaatcgttg tcaatgaatt aagggtttag tcgatagtcg agagtcaata gtcgaaagtc
     8101 tttaatcatc gactaacgac tagtgactag cgactattga ctttgcactt cattcatctt
     8161 ttttcttagt tccttgctag gtctaaagtg aggaagcttc ttggatggaa cttgtactgt
     8221 ttcccctgtt ctgggatttc tgccgctgta gggtttataa acttttgtaa agaaactgcc
     8281 gaagcccctt atttcaatcc tgccaccacc ttgaagcaca tcagtcatgt ggttgataac
     8341 agcatcaatt atctgctcgg actgctgggg ctgaagacca aatttagcgg ataattccct
     8401 ttcaagttct gatcttttca ttttaagacc tcttatttga ttaggatttg gaatccaatt
     8461 tatttaattt gacatctatt gtcaagtgtg aaaactgttt gaaactgttt gctgttgaca
     8521 actctttctg aagtaactat gctttagcga atcctgtaac agttttcgga gcacaacatg
     8581 atgggtagac tcgaaagaat attaaacaaa gcggacaact ttaaagaatc tgaaaggttt
     8641 gaggaagcgc tcagggaact tactaaagca cttaaaatcg cccctcacga tcctgatgtc
     8701 tatctatcct ttgccctaac ttatgatgcg atggaggaat ttgaaacagc cgttgcttat
     8761 ttcaaaaagg cccttgagct tggtccaaaa gattgttaca tcctaacgca atttggaatt
     8821 actctaggaa gaatgggtcg ctatattgat gccctcacgg tttttgaagg cgccctaaca
     8881 attgaaccag gctatgtcgt cgcaaaatgg aacctggctt taaccttgag ggctattgga
     8941 tgttgcgagg acgcactctc aggatttatg caatgtatga caacagacac agactccgat
     9001 tacataaaga gcgaggtcca ctaccagata ggtttatgct actttgatat gggatggaca
     9061 ccagaggcgc tcgttgagct caataaacag gttgaactct tcccagatga tatttgggct
     9121 catctttcaa tcggcaactg ctacttagac tgcggatggg tggatgaatc aataagcaaa
     9181 tacagagaaa tcatcagtgc ttgtcccgat ttcattcccg cttataattc tcttgctctt
     9241 tctttggctg agaaggggtg gtacgatgaa gctctggatg ttttgagggt tgctcttgag
     9301 tatgcccctg aggacgaatc aatcaaagac aatatagatt atatacaatc tttaaaagac
     9361 gacgagaatg gatttaaagg aataataact tactgtttaa tcaaccaaat tctaaagaga
     9421 aggcactcct taaaaaatag ccgttagctt tttgtaattc atcacagaaa ataaccctat
     9481 tcgacagcga tcaaattaaa agcaattatg agtaagcgat ttttctccat attctgtttt
     9541 gcctatctat gaaatgtccc gtttcttttc ttcatactct tctttggtta tttctccacg
     9601 ggcataccgt ttctttagga tctccaaggc cgagttcgca gctttactac gttcacctcc
     9661 tgccctccgg acaacccata ttacaagcag aacaatacca ataatcacga gaacccagaa
     9721 gaccatattg
//
LOCUS       DATDRL010000212         8081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_815875, whole genome shotgun sequence.
ACCESSION   DATDRL010000212 DATDRL010000000
VERSION     DATDRL010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815875"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VI935_07270"
     CDS             complement(<1..602)
                     /locus_tag="VI935_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLE25436.1"
                     /translation="MKIQKILWAYDGSKESDEALNYAVYLAKKFGSEITGVYVSVMPI
                     MSMYLLYPHFESEPFFSLMEQAEKHYKAKLTSIKNELVSQGLRFNGRVIKGEPAKDIV
                     EIAHGEKSDLIVMGKRGLGLIDRVLIGSTTLKVLRESEIPVLSVKKRDEKSTVEIKNI
                     LVPYDISEKNDSALNYAINLGEEVSANISVVCVIRLESYD"
     gene            complement(752..1609)
                     /locus_tag="VI935_07275"
     CDS             complement(752..1609)
                     /locus_tag="VI935_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLE25437.1"
                     /translation="MIHKILWASDGSKDSIQALKHAELIAKKLKAQILSLLVIPDYYD
                     VAEKFPPDEKQRFAKWIEETTTKERKVLEDIGKDFKEKGINFKIEITSGIPHRQILKV
                     ADREKVDLIALGKGRAVERSILGGTALKVLRSSTLPVLTARGDGKSLEIKRILVPTDL
                     SHGLSKDLIYAVELSKVFGATINLLNIVEVGEHAFPPEIVEQMRGFAYREIVESMGKV
                     KIKENIDASVEVAKNAWRGIVGFANERDIDLIVMMSYGGGKLKEDFIGSVAEKVIQES
                     ASPVITMTP"
     gene            complement(1669..1857)
                     /locus_tag="VI935_07280"
     CDS             complement(1669..1857)
                     /locus_tag="VI935_07280"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HLE25438.1"
                     /translation="MAKAREKVPKKPKEKAGQEIETLHEMIEKKAFEIYEKRGKEPGK
                     DLDDWLEAERIIKQRKKL"
     gene            complement(2044..2499)
                     /locus_tag="VI935_07285"
     CDS             complement(2044..2499)
                     /locus_tag="VI935_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HLE25439.1"
                     /translation="MALMKWSPPKEIERWFEDFFEEPFLPRMWRKFPRLRRLRELEEI
                     SPSVDMFDKKDEIVLKAEIPGAEKENISISLSDNTITIKGEMKKEEEVKEEDYYYAER
                     SYGSFSRSLNLPAKVQAEKIKASFKNGILEIHLPKAEESKPKEVKIEVK"
     gene            complement(2983..3186)
                     /locus_tag="VI935_07290"
     CDS             complement(2983..3186)
                     /locus_tag="VI935_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25440.1"
                     /translation="MSKKPIFAGIALDTTGEKQYLGCIKITIIPLPHTYDFNSTTSTL
                     RGRCQGVSYHVCKQIASGYFYLM"
     gene            3396..4283
                     /locus_tag="VI935_07295"
     CDS             3396..4283
                     /locus_tag="VI935_07295"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HLE25441.1"
                     /translation="MKKISNFVFWRKPERKAIRFLDVIFALTLAAFNLVMKTKKKAEW
                     DERIIKQSNKQWGDIIECMAIRPMIFIGLALVIGLLPSAAGAKGSPALNACQASLNTC
                     NADLATCEGDLAASDGFVDNGDGTVTDVRSGLMWEMKTGTVGSSVICTSAAVCPDPHN
                     VNNVYDWADTNFAVFVRQLNNTCDGDPTITCDSDPACGVGDCCGFACHRDWRIPEVGR
                     EPGSIELEGILSFAQGSCGDGIGACISPVFGPTAASSYWSAMTCALDTSFAFGVSFFN
                     GFVFCNNKFTDFVSARAVR"
     gene            complement(5012..5914)
                     /locus_tag="VI935_07300"
     CDS             complement(5012..5914)
                     /locus_tag="VI935_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLE25442.1"
                     /translation="MVRIDKILWTTDGSKEAQEALNYAKVFAKAFNSEIVGLHVIKSI
                     DKRFWGLIGERLDIEGLAEDASIKANWFNVFEGVQEELKREGLKLSFKVLSGAPHEKI
                     IEVANEEKADFIIMGKRGLGLKGRMLIGSNTIKVLQRSQLPVLAVKSRREKTLPRIKK
                     ILVPFDVSEKFDSALRYAVFLSKAFNGIISVIYVLELMSYPYEFPIKILNEIRGLHDD
                     ELKNKIAEIYSQEGNKVKIKHKVIESINPYLGIIRYAEDEKPDLIVMNTHGRSGMKKL
                     MLGSVAEKVIHEAPCSILAIKPTK"
     gene            complement(6000..6383)
                     /locus_tag="VI935_07305"
     CDS             complement(6000..6383)
                     /locus_tag="VI935_07305"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HLE25443.1"
                     /translation="MNKGQFLKRVQDYANINNRKRAEELCKIVFHLLSARLTENESRD
                     LESQLPSGIKEMWEEVEEKGVIKFHKDEFLERIMMEGRLESIVIAEIVAKTVFKVLKE
                     QITKGEANDVAAQLPKDLKEMWFSA"
     gene            6604..>8081
                     /locus_tag="VI935_07310"
     CDS             6604..>8081
                     /locus_tag="VI935_07310"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HLE25444.1"
                     /translation="MELTENALKVLKARYLLKDEKGNVMESPKDMFRRVAHHIAKVEK
                     FYNEDVGYCEESFFNLMTSLKFLPNSPALMNAGKSMGQLAACFVLPVEDSMKSIFDTL
                     KNAALILQSGGGTGFSFSRLRPKADIVRSTGGIASGPVSFMRIYNTATDVIKQGGARR
                     GANMGILRIDHPDIVEFIQVKRNERELNNFNISVAVTDAFMEALKRDLEYDLINPRSG
                     DIVGRLRAKMVFDEIVESAWETGDPGLVFVERINNANPTPHLGQIESTNPCGEQPLLP
                     YEACILGSMNLSRYVKDGDIDYDSLGSDIRIAVRFLDDSIDANIYPLPEIERMHRGNR
                     KIGLGVMGWADMLIHLRIPYNHSKAFDLGRRIMKFISNKSREASAELAEKRGVFPNFK
                     GSIYDTPGMPRVRNATTTTIAPTGTLSLIADCSSGIEPLFAIAYKRFVLDTELCEINK
                     YFLEFAQKMGFYTDEVMNRVIKSGNLKGIKEIPKDIRRLFKTA"
BASE COUNT         2178 a         1706 c         1595 g         2602 t
ORIGIN      
        1 aaatcatagc tttctaatct aataacgcac actactgaaa tattagcgct tacttcctct
       61 ccaagattga ttgcgtaatt gagggcagag tcgttttttt cagatatgtc ataaggaaca
      121 agaatgtttt ttatttccac ggtgcttttc tcatccctct tctttactga aagaacaggt
      181 atttcagatt ccctaagaac tttaagcgtt gtacttccaa ttaacaccct atctataagt
      241 ccaagtccgc gtttccccat aactataagg tcagattttt caccgtgggc gatttcaaca
      301 atgtccttag ccggttctcc ttttataacc cttccattga aacgaagtcc ttgagaaact
      361 aattcgtttt taatcgaggt tagctttgct ttataatgtt tctcagcctg ttccatcaag
      421 ctaaagaatg gctcgctttc aaaatgtggg taaagtaagt acatagacat tataggcatc
      481 acgcttacat aaacacccgt tatctcagag ccgaatttct tagcgagata tacagcatag
      541 ttaagtgcct catcggattc cttcgaacca tcataagccc agagaatctt ttgtatcttc
      601 attactacct cctcccgtag tatgcaacaa ttttctaata aaattaagga ctttgtttta
      661 tggtttgtta gaagcgcttc gccagttaac ggtattggag gtgtcacagg gttcataata
      721 ggctttagcg ttcgaaggct tgcgggaggg attaaggggt catcgtaata acaggagaag
      781 ctgattcctg aataaccttc tcagcaacgc taccaataaa atcctctttc agctttccac
      841 cgccatagct catcataact attaaatcta tatccctttc gttagcaaat cctactatgc
      901 ccctccaagc attcttggca acctctacgg aagcatcgat attttctttt atttttacct
      961 tccccatact ctcaacgatc tccctatagg caaaccccct catctgttcg actatttcag
     1021 gcggaaatgc atgctctcca acctcaacga tgttgagtag attaatcgta gcgccaaata
     1081 cctttgataa ctcaactgcg taaattagat cttttgatag tccgtgagat aggtctgtgg
     1141 gtactaatat cctttttatc tccaaactct tgccatctcc cctagcggtt aataccggta
     1201 gggtagaact cctaagaact tttagtgcag tgcctccgag gatagacctt tcaactgctc
     1261 ttcccttccc caaagctata aggtctactt tctctctatc agctactttt agaatctgtc
     1321 tatggggaat gccactcgtt atttctatct taaagtttat tcctttttcc ttgaaatctt
     1381 tcccaatatc ctcaagtact ttcctctctt ttgtagtagt ttcctcaatc catttagcaa
     1441 atctctgctt ttcatctgga ggaaactttt ctgctacatc atagtaatca ggtattacca
     1501 aaaggcttaa tatttgcgcc ttcaactttt tggcaatgag ttccgcgtgc ttaagggctt
     1561 ggattgagtc tttagatcca tcagaagccc atagtatttt atgaatcatt ataaggcctc
     1621 ccttctgctt ttattatttt ctttaacccc tttttaatta caatctattt aaagcttctt
     1681 tctttgtttt attattcgct ctgcttcaag ccaatcgtca agatctttgc caggttcttt
     1741 tcctcttttt tcatatatct caaaagcctt cttctcaatc atttcatgaa gtgtttctat
     1801 ctcttgcccg gctttttctt taggcttttt tggcaccttc tctcttgctt ttgccattat
     1861 attcctcctc agtctaaata cacaagtatt tctatcatga gaatagatat tatctaacta
     1921 acttgtgaaa tcattttgga atctgcgctt tttacaagtt gcttaaaatg actataagag
     1981 caagactcct gtatattaaa aatggagagc ttattttgca agagctcccc attcttaagt
     2041 gttttattta acctcgattt ttacctcttt tggctttgat tcttcagctt tcggtaggtg
     2101 tatctcgagt ataccattct taaaacttgc ttttattttt tctgcctgca ctttagctgg
     2161 tagattcaac gatctcgaaa aactgccata tgacctttct gcgtaatagt agtcttcctc
     2221 ttttacctct tcttccttct tcatttctcc cttgatggta attgtgttgt ctgagagaga
     2281 gatacttata ttctcttttt cagcacctgg gatctccgcc ttaagcacta tctcatcctt
     2341 tttgtcgaac atatcaactg atggagagat ttcttctagt tctctaagtc ttctcaacct
     2401 ggggaatttt ctccacatgc gtggcaggaa aggttcttca aaaaaatctt caaaccacct
     2461 ttcaatttcc tttgggggtg accatttcat aagtgccatg atatttacct cctattttta
     2521 gtctttactt attatttata tgcaaatgct ataccaattg taaatagatt aattgaataa
     2581 cgataaaagg aagtttaggc tacacataaa attaaacact gtgtcagttg tcagaaaatt
     2641 ggacaatgat tctttctgtc attccgagta aagcgaggaa tctcgttttt agattgcctt
     2701 atcgcggagt ctatcctgaa cacttcggct tcgctaaggg caggctcagg cgaacatgcg
     2761 aacggtattt cgaccaaccc gttcatgctg agcctgtcga agcatgagct gtgagcttgc
     2821 cgaacagttg aaccatgaac agcgattata attaagtcat aattgataaa aatttattta
     2881 actttctaca agtaacttag gcttcggtgg ttttcatttg gcaaaggttt ttctcaattc
     2941 tgtctcttat atttaaatgt cagtgacatt attactttgc aactacatga gataaaaata
     3001 cccacttgca atttgtttac atacgtggta tgaaacccct tgacatctac ccctaagtgt
     3061 gcttgttgta gaattaaagt cataggtatg aggaagggga atgatggtta tttttatgca
     3121 ccctaaatat tgtttttcac ctgttgtatc gagtgcaatt ccagcaaaaa tgggtttttt
     3181 actcattttt aaaacctact aatttactcc gagtggaatt tctacctacc aaattccata
     3241 ttctgagatt aggcctttcc cttctgtctt atttaggatt ttaaaaaagc accctgggtt
     3301 tggattcgaa aggaggtggt gtcccttgat tgaactaaag attttcaaga acctacaagt
     3361 caagttacaa acttaaagta aaggaggtaa tttctatgaa aaaaatatca aattttgtat
     3421 tttggcgaaa gccggagaga aaagcgataa ggtttctgga cgttatcttt gctctgaccc
     3481 tggcggcctt caaccttgtt atgaaaacta agaaaaaagc cgaatgggac gagagaatta
     3541 tcaagcagag caacaaacaa tggggtgaca tcattgaatg catggcgatt cgacccatga
     3601 tcttcatagg tctcgcgttg gttattgggc tgctgccgtc ggctgcggga gcgaagggat
     3661 cccccgccct caatgcatgt caggcgagtc ttaatacctg caacgccgat ctggccacct
     3721 gcgagggtga ccttgcggca tccgacggct ttgtagataa cggagatggg acggtgacgg
     3781 acgttaggtc agggcttatg tgggagatga agacgggtac tgtgggctct tctgtcattt
     3841 gcactagtgc agcagtctgt cccgacccgc ataacgtgaa caacgtgtac gactgggctg
     3901 atacgaattt tgctgtgttt gtgcgccaat tgaacaacac atgtgacggg gatccaacaa
     3961 ttacttgcga cagcgatccg gcctgtggag taggggattg ctgtggtttt gcttgtcacc
     4021 gtgattggcg gatacctgag gttggtaggg agcccggttc aattgagctg gaggggattc
     4081 tgagttttgc acaggggtcc tgcggcgatg ggattggtgc ttgcatcagt ccggttttcg
     4141 gcccgactgc cgcttccagc tactggtcgg ctatgacctg cgccctcgat accagcttcg
     4201 cctttggcgt ctccttcttc aatggcttcg tgttctgcaa caataagttc accgacttcg
     4261 tcagcgcccg tgcagtgcgt tgatgctcgt gattggtcat ttgatgattt gcgtatttga
     4321 tcatttggtc gccggggatg tggggtggag ttagagccta acccgttcat gctgagcact
     4381 tcggcatagc tcagcacaag cctgtcgaag tatgagcctt tggaggcatg aaattgattg
     4441 taaaactaaa ggcgcacttc gaccgggctc agtgctaacg gtatacaagc attgtaggca
     4501 cgaatttatt cgtgccaaaa atagcacaga caagtctgtg cctacaatat ttaagcctag
     4561 cagaaatagt tatatggcag ataatctccg gagaggggat aatggctcaa gcagagcatc
     4621 tacctattta caagagtgca tatgatctgt gtctttactt cgagcaggtg gtgagaaatt
     4681 tctcacgtta ccactatgcc tttagagctg gttttccggt ctacctttcc cagttgtaca
     4741 aagcacgggc gctccggcat gggttgaccg taatcaatgt gcgtcaagta tctgcgcctc
     4801 ttaatcgggg ccttacacag cgcttgccct gctccttgct aatcccgtca ttgactgttt
     4861 gattcttagt ctgcttgctg tgggctcctc gtttaaagag agaaataaaa accaaggata
     4921 aagactctag ataaacattt agaagcctat taaacacttc aaatgtgcat caaagagggg
     4981 gtaacagctg aaagcatttt aaacttctgg cttattttgt gggtttaatt gccaaaatag
     5041 aacacggtgc ctcatgtatt accttttctg caacgctccc taacataagc ttcttcatgc
     5101 cacttcgtcc gtgggtattc ataactatga gatctggttt ctcatcctct gcataacgaa
     5161 taattcctag gtaggggttt attgattcga ttaccttatg cttaattttt actttattac
     5221 cttcttgaga atatatttca gcaattttat ttttaagctc atcatcgtgt agcccccgaa
     5281 tctcatttaa tatctttatt gggaattcat aaggatatga cataagctct aatacataga
     5341 tcacagaaat aattccatta aatgctttgc ttaaaaaaac tgcgtatctt aaagccgagt
     5401 caaatttctc agatacatca aacggtacaa gtatcttttt tatccttggc agtgtttttt
     5461 ctctcctgct ttttactgcg agtacgggca attgagatct ctgtagcact ttaatagtgt
     5521 tactgccgat taacattcga cccttaagcc ctaaacctcg tttccccatt ataataaagt
     5581 ccgctttctc ttcattagca acttcaataa tcttttcatg cggtgccccg gataaaacct
     5641 taaatgagag ttttaatccc tccctcttaa gctcttcttg aacaccttcg aacacattaa
     5701 accaattagc tttgattgag gcgtcttcag ccaatccttc tatgtctaat ctctcaccaa
     5761 taagacccca gaaccttttg tcaatggact taattacgtg aagccctaca atttctgaat
     5821 tgaacgcttt cgcgaaaact ttagcgtagt ttaatgcttc ttgagcctct tttgatccat
     5881 ccgttgtcca aagtatcttg tcaattctta ccatttgtgt cccccgctat tataatggaa
     5941 taaatattgt actatatcgt gcaatatgat agcatctcta attataaaac ataacaagtt
     6001 caggctgaaa accacatctc ttttaggtcc ttaggtaatt gagcggcgac atcgttcgcc
     6061 tccccctttg tgatttgctc tttaagaacc ttaaaaacag ttttagctac tatctccgct
     6121 ataactatgc tttctaaccg cccttccatc ataatccttt ctaagaactc atccttgtga
     6181 aactttatca ctcccttttc ttcaacctct tcccacatct cctttatccc tgatggaagc
     6241 tgtgattcca aatctctgct ttcgttctct gttaatctgg cactcagaag atgaaaaact
     6301 atcttacaaa gctcctctgc tctttttcta ttattgatgt ttgcgtagtc ttgaaccctt
     6361 tttaagaatt gtcccttatt cattttgccc ctcctaaatt acaattggat tttgcctgta
     6421 tatatgtaat atggcaattt ttttaccaaa agtttaaatt ttgggctgaa aaatactttt
     6481 aatggtaatg tgtcaatttg ggtaggataa ataattagaa aaaaggaagt atgggaagat
     6541 agccttagct tatttctcac atataatatt tctgtgtacg aaatggcata atataattca
     6601 gtcatggagc ttacagaaaa cgctttaaag gttttgaagg cacgttacct tttgaaagat
     6661 gaaaagggga atgtcatgga atcgcctaaa gacatgttca ggcgtgtcgc tcaccatata
     6721 gctaaggttg aaaaattcta taatgaggat gttggttact gtgaggaaag tttttttaat
     6781 ttgatgactt ctcttaaatt tcttcccaac tctcccgccc tcatgaacgc tggtaaatcc
     6841 atgggacagc tcgctgcatg ctttgtcctt ccggtggagg actccatgaa gagcatattt
     6901 gacacattga agaatgcagc tttgatactc cagagtggtg gtggaacagg tttttccttc
     6961 tcaagattga gaccaaaggc tgatattgtg aggtcaactg gcggaatagc cagtggacct
     7021 gtttctttta tgaggattta taatacagct actgacgtta taaagcaggg tggtgcgaga
     7081 cgaggggcga acatggggat tctcaggata gaccatcctg atatagtcga gtttatccag
     7141 gtcaaaagaa atgagaggga attgaacaac tttaacatct ctgttgctgt gactgacgca
     7201 ttcatggagg cattgaaaag agacctagag tacgatctca taaatccaag gtcaggagat
     7261 attgtaggaa gactcagagc aaagatggtg tttgatgaga ttgttgaaag cgcatgggag
     7321 acaggggatc ctggattggt attcgtagag aggattaata atgcgaatcc aacgccgcac
     7381 ctaggccaga ttgaaagcac aaacccctgt ggggagcagc cgcttctacc ttatgaggca
     7441 tgtatattgg gttccatgaa tctctcaaga tatgtcaagg acggggatat cgattacgat
     7501 tcacttggca gcgatatcag gatagctgta cggtttttag atgattcgat agatgcaaat
     7561 atttatcccc ttcctgaaat tgagaggatg cataggggaa acaggaagat tgggctcggt
     7621 gtaatgggtt gggctgatat gctaatacac ttgagaatcc cttacaatca tagcaaggca
     7681 tttgatctcg gaaggaggat aatgaaattt ataagtaaca agtcaagaga ggcatcggct
     7741 gaactggctg aaaaacgggg ggtcttcccg aattttaaag gctcgatcta tgatacacct
     7801 ggtatgccca gggtaagaaa cgcaacgaca accaccattg cacccactgg aaccctttcc
     7861 ctaattgctg actgctcaag cggtattgaa cctctatttg caatcgccta taagaggttt
     7921 gttcttgaca cagaattatg cgagataaac aaatactttc ttgaatttgc ccaaaaaatg
     7981 ggattttata cggatgaagt aatgaatagg gttataaaga gtgggaatct caaagggata
     8041 aaggagatac caaaggatat tagaaggcta tttaaaacag c
//
LOCUS       DATDRL010000213         5885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_813231, whole genome shotgun sequence.
ACCESSION   DATDRL010000213 DATDRL010000000
VERSION     DATDRL010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813231"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VI935_07315"
     CDS             <1..306
                     /locus_tag="VI935_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HLE25445.1"
                     /translation="SMTKVSIQRRGNSMAKRISAMDLRKRLGQIMNEVTIRDDEYIIE
                     RDGKPMAALIPLWKLKQLEERKETFWKKVKEFRREGKRVQKKELEPAISEAVKAAKG"
     gene            320..757
                     /locus_tag="VI935_07320"
     CDS             320..757
                     /locus_tag="VI935_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HLE25446.1"
                     /translation="MFRVVLDANQFVSAILNQDGPPAKVLNAWREGLFELVISPSIID
                     EIRKVLNYPRLSKIHKKSPKEIDLFLEDLEILAFVAPEKLSLSVVLDHPTDDKYLVAA
                     LEGDAKFIVTGDSDLLTIQEYEGVKILTARAFLKEMELTRRKK"
     gene            complement(1042..1118)
                     /locus_tag="VI935_07325"
     tRNA            complement(1042..1118)
                     /locus_tag="VI935_07325"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1082..1084),aa:Ala,seq:cgc)
     gene            complement(1368..2855)
                     /locus_tag="VI935_07330"
     CDS             complement(1368..2855)
                     /locus_tag="VI935_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLE25447.1"
                     /translation="MPFPTETIKRYIAARYPILYLVSLEEDRVERMLSLLGARLFTTP
                     IKFYTWSCINGLRSNGIEIENTNDLIQALDRITREEETGFYLFKDLHFFLNDPRLIRK
                     LREVYRALRTSYKTLFILSPSLILPNELEKEVTVIDIGLPDVDEAGKVFDFVINSNKD
                     FEKIRSSLNPTIREKCIRAMLGLGSMEMELALIKILSGRESMGEESIEAILEEKAQLV
                     RKSGTLEFVRARIGLDDIGGLENLKEWLQTRALAFSNEAKKFGLDMPKGVLLMGISGC
                     GKSLSAKAVATAWDLPLLRLDLNQVYSGAFGSPEEAFRRSIKTVEAIAPCVLWIDEIE
                     AGITRSGEKASDSPASRIFGYFLTWMQEREHAVFITATANQIDLLPPEILRKGRFDEI
                     FFVPLPSHKERKEIFRIHLLRRGRNPDSFDLDSLAKNTDGLSGAEIEQTIVTALYESF
                     SMGKELDERELIIAASSIVPLSTTMREEISKLERWSSNRAVKASK"
     gene            complement(2943..4487)
                     /locus_tag="VI935_07335"
     CDS             complement(2943..4487)
                     /locus_tag="VI935_07335"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HLE25448.1"
                     /translation="MDSGVVKIFDTTLRDGEQAPGCGMTAEEKLRVAYQLEKLGVDVI
                     EAGFPVSSEGDFQSVKLISEDIRSCEIAGLCRANFNDIDRAWEAVKRAENPRIHTFIA
                     TSNIHLKHKLGKTRDEVLEIIGAAVKHAKRYTENVEFSCEDATRTELDYLCKAVDIAI
                     KAGAITINIPDTVGYTVPEEFSYIIRTLKERVQDLDKVTLSVHCHNDLGLAVANSIAA
                     IKEGARQVECTVNGLGERAGNASLEEIVMALKVRHDRNPFKTRIVTEQIYPTSRLVAQ
                     VTGVNVQPNKAIVGANAFAHEAGIHQDGVLKERITYEIMKPEEVGIPSNKIILGKHSG
                     RHAFKRRLEEFGYFLNESNFQRAFQRFKDLADKKKYVFDEDIEALISDEFIRSSDFYR
                     LISANFASGTDMRPVATLRLLIDGEEVTVSEYGDGPVDATYKAISKATGITPNLEHYI
                     VAAITGGTDAQGEVTVRIEEDGIVSQGLGASTDILVASAKAFINALNKLRWRKEHPKQ
                     ASTKGV"
     gene            complement(5118..>5885)
                     /gene="aroE"
                     /locus_tag="VI935_07340"
     CDS             complement(5118..>5885)
                     /gene="aroE"
                     /locus_tag="VI935_07340"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HLE25449.1"
                     /translation="AAFQSLRLDCVYLAFDVHPKDFEIAARAIRSMCISGVNITIPHK
                     ESIIFFLDEISPEASLTGAVNTVKNADGKLIGYNTDVSGFLRAIKEDLGFHPKGLRAA
                     LIGAGGAARAVLTSLCMNEAYQVYVINRTLDKAKRLASEFKKGFKDTFIQAVSLDDKD
                     EIKNILGKTNLLVNATSAGMKGVNSVEMPLEHLQKEAVIYDLVYDPRETNLIKTAKEL
                     GHKASGGLSMLLYQGVESFEIWTGKDAPTGIMKKAIE"
BASE COUNT         1640 a         1361 c         1130 g         1754 t
ORIGIN      
        1 tctatgacta aagtttctat acaaaggagg ggaaacagta tggcaaagcg catatcagcc
       61 atggacttga gaaaacgcct tggacagatt atgaatgaag ttaccatacg ggatgacgag
      121 tacatcattg aacgggatgg aaaaccaatg gctgccctta tacctctctg gaagcttaaa
      181 caacttgaag aaaggaaaga aacgttttgg aaaaaggtaa aagagttcag gagagaagga
      241 aaaagggtgc agaagaaaga acttgagcca gccatatccg aagcggtaaa agcggctaag
      301 ggttaataac gctactccta tgttcagggt agttctcgat gccaatcagt ttgtcagcgc
      361 tattcttaat caggatgggc ctcctgcgaa agtcctcaac gcctggcggg agggcctttt
      421 tgaattagta atctcgccat ccatcataga tgagatacgg aaggtactca attacccaag
      481 actttcgaag atccataaaa agagccctaa agaaattgac cttttcctgg aagatcttga
      541 aatccttgct tttgttgcac ccgaaaagct ttccctgtct gtagtgctgg atcaccccac
      601 agatgataaa tatcttgtag cagccctgga gggagatgca aagtttatcg ttactgggga
      661 ttccgatctt cttacaattc aagaatatga aggtgttaaa atacttacag caagagcgtt
      721 tctcaaagaa atggaactaa cgagaagaaa gaagtaatac tatttccaaa tccatgactt
      781 cttctaagta taaacgtata gctttttgaa catggaaact tcgacttttg gatgtacact
      841 ttgttactct atcaagctcc tctaacaaat tatacggaat cttgatttct gctaatccca
      901 ttgttatcac cttttttaat ctattgttaa atatatcata cagtcatgat gtaatgatag
      961 cagttccaat tacaaagtaa aaataacttt taggatttta ggctcgtttt agagtccaaa
     1021 tgaaccttac taattaatag atggtggaga tgacgggaat cgaacccgcg acctctgcct
     1081 tgcgaaagca gcgctctccc aagctgagct acatccccac ttttaaaatg atccaagata
     1141 catgattcat gatgcatgag gttttagtgc atcttgcatc ctgtatggtt ccccaattct
     1201 ataacataac ttagcatagc cttcaagcac ccaaatttgc ctattgtgat ttctgaatag
     1261 ttgacccata gtgtttaact acaacgttca tagaaggcac agaaggattt tttttatttt
     1321 taatagattc tctgtgatct ctgtgtccca aattataagc cacgaaatca cttagaagcc
     1381 ttcacagctc gatttgatga ccagcgctct agctttgaaa tctcttctcg catagttgtg
     1441 gataggggaa cgattgaaga cgccgctatt attaactccc tttcatctag ctcctttccc
     1501 atagaaaatg attcatagag cgctgtgaca atagtctgtt caatttcagc gccactaagc
     1561 ccatccgtat tcttggcaag cgagtctaaa tcaaaggagt ctggatttct tccacggcga
     1621 agaagatgga ttctaaatat ctcctttctt tctttgtgag atggaagcgg gacgaaaaat
     1681 atctcgtcaa atcttccctt tctcaagatc tcgggaggaa gcaagtctat ctggtttgca
     1741 gtagcagtta taaagactgc atgctccctt tcttgcatcc aggtaagaaa ataaccaaat
     1801 atccttgatg ccgggctgtc gcttgccttc tcaccgcttc ttgttatccc ggcctctatc
     1861 tcgtcaatcc aaagaacaca gggcgcaatc gcctcaactg tctttataga ccttctaaaa
     1921 gcctcctcgg gactaccaaa agcgccactg taaacttgat ttaaatcgag tctcagtagt
     1981 ggaaggtccc atgcagtagc tactgcttta gcactaagac tcttcccaca accgctaata
     2041 cccattagta gaacaccctt tggcatgtca agcccgaact ttttggcttc attagaaaag
     2101 gctagagccc ttgtttgaag ccactctttg aggttttcaa gccctccgat atcatctaag
     2161 ccgatacggg ctcttacaaa ctcaagtgtt ccggatttcc tcacaagctg agccttttct
     2221 tccaaaatcg cttcaattga ctcttcgccc atgctttctc ttcccgaaag aatttttatt
     2281 agggcaagct ccatctccat tgatccaagc ccgagcattg ctcggataca tttttcccta
     2341 attgttgggt taagcgagct tcgaatcttc tcaaagtctt tattggaatt tattacaaaa
     2401 tcaaaaacct ttccagcctc atctacatcc ggaaggccga tgtcaattac tgttacctct
     2461 ttttcaagct cgttaggaag aattaaagaa ggagaaagta tgaagagggt tttgtagcta
     2521 gttctgagag ccctataaac ttccctgagc tttcttatta atctgggatc gttaagaaaa
     2581 aaatggaggt ccttaaagag ataaaatccc gtctcttctt ctcttgtaat ccggtctaaa
     2641 gcctggatca gatcattcgt attttctatt tcaattccgt tgcttcttaa cccgtttata
     2701 caggaccaag tataaaactt aattggggtt gtaaaaagcc tcgctccaag aagtgataac
     2761 atcctctcaa ccctatcttc ctccaatgat acaagataga gaatagggta tcttgcagca
     2821 atgtatcttt tgattgtttc ggtggggaaa ggcatttttg tgactcggta ttcgtgacta
     2881 gtggttcgtg gttcgtgatt cgtgattcga tacaagcttc aacgatacac aaaccacgaa
     2941 ctctaaaccc ccttagtaga ggcttgtttt ggatgctctt ttctccagcg gagtttattc
     3001 agtgcattta taaaggcttt tgcactcgcg actaaaatgt cagtgcttgc acccagcccc
     3061 tgagaaacta taccatcctc ctctattctc actgtaacct cgccctgcgc atcggtgcca
     3121 cctgtgattg ctgctacaat gtaatgttca agattaggtg ttattcctgt tgcctttgag
     3181 atggctttat atgttgcatc aacaggacca tccccgtatt cagacacagt tacctcttct
     3241 ccatctatca aaagccttag agtcgcaaca ggccgcatgt cagttccact tgcaaaattt
     3301 gcagaaataa gcctgtagaa atcagacgaa cggataaact catcactaat tagcgcttct
     3361 atgtcctcat caaatacata tttcttctta tcagccaagt ccttaaacct ttgaaatgct
     3421 cgctgaaaat tgctctcatt aagaaaatac ccaaattcct caagacggcg tttaaaagca
     3481 tgtcttccgg agtgctttcc aaggattatt ttatttgaag gaatccctac ctcctctggt
     3541 ttcataattt cgtacgttat tctttctttg agaacgccat cttgatgaat gccagcctcg
     3601 tgtgcgaagg cgtttgcccc tactatcgcc ttatttggct gaacattaac tccagtaacc
     3661 tgagctacaa gcctgcttgt tggataaatc tgttcagtaa cgatcctagt tttaaaagga
     3721 ttacggtcat ggcgcacctt aagagccatc acgatctctt caagtgaagc gttccccgcc
     3781 ctttctccaa ggccattaac agtgcattca acctgcctcg caccctcctt aattgcagca
     3841 atagagtttg ctacagccag tcctaggtcg ttatgacaat gcacacttag tgttacctta
     3901 tcaagatcct gtactctctc cttcaaggtt cttatgatat aggaaaattc ctcaggcact
     3961 gtgtatccaa ctgtatccgg gatgttaatc gttatagctc cagcctttat agcaatatct
     4021 acagccttgc ataagtagtc aagctctgtc cttgttgcat cctcacatga aaactctaca
     4081 ttttcggtat agcgctttgc atgtttaacc gctgcaccta tgatttctaa tacttcatcc
     4141 ctagttttac caagcttatg tttcaagtga atattagagg ttgcgataaa cgtgtggatc
     4201 ctcgggtttt cagctctttt tactgcctcc caagccctat ctatatcgtt aaagttagcc
     4261 ctgcaaagcc ccgctatttc acatgatcta atgtcctctg aaatgagctt tactgattga
     4321 aaatcccctt cagatgaaac tgggaaacca gcctcgataa catccacacc taatttttca
     4381 agctgatatg ctaccctaag cttctcctca gcggtcatac cgcagccagg cgcttgctct
     4441 ccgtctctaa gggttgtatc aaatatcttt actactccgc tatccatttt taagaagcct
     4501 ccatttataa aaacagttaa aaagtttaat cattatggaa agggttgtca atttgaataa
     4561 atctcagtct tttttaggtg aatcaatctt taaactaatt gtaaaaatag gattttagta
     4621 gagacgaccc ggtgggtcgt ctctacataa ggtggtctgc agcccacgaa taatgcccgc
     4681 aacgactggc ggggttagaa aaccccgcct atcctgaatt agaagcgaga ttcctcactg
     4741 ctccttcgtc ttcgctcagg acatggttcg gaatgacaat ttgtgttgga tcgctttgca
     4801 tcgtaggggt ttgattaatc aaatccctac agctcgtaat aacgggaaag gattatcgct
     4861 gtcatgtagg ggcaggttta aaccctgccc ctaccgatga agtaagggtg tggaggccta
     4921 aagccctccg ctacatttat cccgttatgt tgatgttgca aaatcaggag ggctggggca
     4981 cgttgcaagg tacccctaca tcgttaggga aaaaatgaaa taaagacggg ctgaacggtc
     5041 ccttcgacag gctcaggaca gggttcagcc cctacaattg aaggaccata caattgcaaa
     5101 acttacatca ttttaattta ttcaatcgcc tttttcatga tgccagttgg agcgtcttta
     5161 cctgtccaaa tctcaaagct ttctaccccc tgatagagaa gcatgctcaa tcccccagat
     5221 gccttgtgtc ctaattcctt cgcagttttg atgagatttg tctcacgggg atcataaact
     5281 agatcataga tcaccgcctc tttttgaagg tgctctaatg gcatttcaac tgaattaact
     5341 cctttcattc ctgctgatgt agcattcaca agaaggttag ttttacccag aatatttttt
     5401 atctcgtctt tatcgtcgag agaaacagct tgtataaagg tatctttaaa acccttttta
     5461 aactccgagg ccaatctctt tgccttatcc aaggttctat taatcacgta aacctggtac
     5521 gcctcgttca tgcaaagaga tgtgagaacg gcacgcgctg caccacccgc tcctatcagg
     5581 gctgccctta gcccctttgg atgaaaacct aaatcctcct ttatcgctct tagaaaaccg
     5641 ctgacatcgg tgttatatcc tatgagtttt ccatccgcgt tttttacagt gtttacagcg
     5701 ccagtaagac ttgcttcagg ggaaatttca tccagaaaaa aaataatgct ctctttgtga
     5761 ggaatcgtaa tatttactcc agaaatacac atagacctta tagcgcgagc ggctatctca
     5821 aaatcctttg ggtggacatc gaaagcgaga taaacacagt cgagtcgtag tgattgaaaa
     5881 gcagc
//
LOCUS       DATDRL010000214         4684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_692859, whole genome shotgun sequence.
ACCESSION   DATDRL010000214 DATDRL010000000
VERSION     DATDRL010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692859"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VI935_07345"
     CDS             complement(<1..684)
                     /locus_tag="VI935_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HLE25450.1"
                     /translation="MNKTIIKKFGLFAASFALSLIAVLAGSKLYANLLGIHDSSFRYE
                     NQLNMWRSEPTIGFLNKPNFSSFCFGNVRVETNERGFRGTRPSPFAKTIGVLRIVGLG
                     DSVMWGTGVNEENSILGFLEKKLNNESLYEVINAGVVGYSTYQELLYLKKYVIPLKPD
                     VVLVNYSENDLLPTEDPFKNVRRIYIRYLNHLLESHHLALTPEEKLGIKELIRIFNTA
                     KYVWFTLHGL"
     gene            complement(1127..1750)
                     /gene="hisG"
                     /locus_tag="VI935_07350"
     CDS             complement(1127..1750)
                     /gene="hisG"
                     /locus_tag="VI935_07350"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HLE25451.1"
                     /translation="MITVAISRGRILKEATGLLRKAGFSVSGISNGSRKLIYEYPKDG
                     IKILIIRPTDVPPYVEYGAADCGIVGKDTLIEEKYDLYEPLDLGIGQCKLVVAGPKGF
                     NLSSTRILRVATKYPRIAHEHFAKKGISAEVVKLYGSIELAPIVGLSDVIVDLSATGE
                     TLRKNKLVEIETIANITARLVVNRVSMKLQSNEIKSFIGRLKEVREI"
     gene            complement(1747..3003)
                     /gene="murA"
                     /locus_tag="VI935_07355"
     CDS             complement(1747..3003)
                     /gene="murA"
                     /locus_tag="VI935_07355"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HLE25452.1"
                     /translation="MEKIIVEGGKRLEGEVTISGAKNAVLPLIASCILTEGRSTFTNV
                     PELDDVKTMSKLLRILGAEVEYEKGTLSIDTTRLNNWVAPYELVKTMRASVLVLGPLV
                     ARFGHARVSLPGGCAIGERPIDQHLKGLGILGADIKIEEGYIEATSPKLNGSVIPFDI
                     STVTGTENIVLASVLAQGETMLLNAACEPEVTELVHVLKKMGAEIEGVGTDIITIRGV
                     DKLSPINGHEVMPDRIEAGTFLIASALTQGDILIKKCRFENSGALIGKLLEVGAEVSR
                     IDSGIKVRGSDKIKSIDITTLPYPGFPTDMQAQMMTLMSVADGLSIITETIFPQRFMH
                     AGELRRMGADIKLVGNCAVVRGVPYLSGAPTMAGDLRASASLVLAGLLARGKTQVSRA
                     YHLDRGYDRLDKKLEALGAHIWREKE"
     gene            complement(2991..3929)
                     /gene="prmC"
                     /locus_tag="VI935_07360"
     CDS             complement(2991..3929)
                     /gene="prmC"
                     /locus_tag="VI935_07360"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HLE25453.1"
                     /translation="MKELYLKGRDLLKKSGVENPEFEAALLLSKVLRINTTYIYAHPK
                     NWVQSDKIKELELLLERRIKREPIAYILGEKEFYSRSFIVTPQVLIPRPETEILVEEA
                     LKITKTTPNSLILDVGTGSGCIAVTIGCESENAQIFAADISLEALMVARANSVRHKVA
                     ERISFICADFLSCFKGNSFDIIVSNPPYIPESEFHRLESDIRDFEPKISLIGGGSILR
                     HGSGQALRLHPSTVRSELVEDSRSTSGEEGLNCIKKIVHEAGRVLKNGGWCIIEIGAN
                     QSEKVLETFECIGFKEVSTVKDLADIERVVKGKWKR"
BASE COUNT         1261 a         1050 c          916 g         1457 t
ORIGIN      
        1 caatccatgc aacgtaaacc aaacgtattt tgcagtgttg aagatgcgaa tgagctcctt
       61 aatgcccaat ttttcttcag gggtgagtgc taagtgatgg ctttcgagaa ggtggttgag
      121 atatcgaata taaattcttc gcacattttt gaatgggtct tcggtcggca aaaggtcgtt
      181 ctcagaatag tttacaagaa cgacatccgg ttttagtgga atgacgtact tcttaaggta
      241 caacaactcc tggtaagtcg aatatcccac gacacctgcg ttgattactt catataatga
      301 ctcgttgttc aatttcttct caagaaatcc tagaatagag ttctcctcgt ttacaccagt
      361 accccacata acggaatcgc ccagcccaac gattcgaagt actccgattg ttttcgcgaa
      421 cggtgaaggc cgagtaccac ggaaaccgcg ttcgtttgtt tcaaccctga cattaccgaa
      481 acagaagctt gaaaagttag gcttgttgag aaaaccgatt gtgggttcag aacgccacat
      541 attgagctgg ttctcgtaac ggaaagacga atcatgaata cccagcaagt tggcatacag
      601 cttagagcct gctagaacag cgatcaggga taaagcaaag ctggcagcga aaagtccgaa
      661 tttcttaata atggtcttgt tcataatagt accaatcccc tatagactag actgttgtca
      721 aactagactc gtaactgact cgctttcacc attaggttat tccaagcgcc taactagtaa
      781 ttatacgagc aaaagttttt ataatcaccc catttggagt gttatccgaa aaaccttgaa
      841 cctcttcctt tgtttcgaaa tatacttaaa aatagctacc cgtctcaata ggaacaagaa
      901 aattatggaa caatctagtg gatcagaaaa gtatggaagt gtagtgaatg atatagcaag
      961 gagaaaccat gattgtcacc atacaccaac ctatgttgtt actctggccc ggattgttct
     1021 ttaaggctat gaaggctgac tgcatgattt tacttgatga cgtgcgattt cctcaaggtc
     1081 gtggcttggt taaaggtttt cgagaaaaaa aggggctcac cactttttaa atctcccgaa
     1141 cctctttaag ccttcctata aagcttttta tctcgtttga ttgtagcttc atactaactc
     1201 tgttaacaac aagcctcgct gtaatattag caatcgtttc gatctcgacg agtttattct
     1261 ttctcagggt ttctccagtg gcactcaggt ctactattac atctgaaagc ccaacaatag
     1321 gagcaagctc gatagaccca tagagcttta ctacctcagc gcttataccc ttttttgcaa
     1381 aatgctcgtg tgcgattctt ggatattttg tcgcaactct caggattctg gtggatgaca
     1441 aattaaaccc ttttggacct gcaacgacaa gcttgcattg tccaatacct agatcaagcg
     1501 gttcatacaa atcgtatttt tcttcgataa gtgtatcctt tcctacaatc ccacagtctg
     1561 ctgccccata ttctacataa gggggcacat ctgtgggacg tatgatcaat atctttattc
     1621 catcctttgg atactcgtaa ataagctttc ttgagccgtt agagattccg cttactgaaa
     1681 aaccagcttt tctcaaaagc cctgtagctt cctttagaat cctccctctt gagattgcaa
     1741 ctgtgatcat tccttttctc tccagatgtg cgcaccaagg gcttcgagtt ttttatcaag
     1801 cctatcatat ccacggtcta ggtggtaagc ccttgaaact tgcgtttttc cacgagctag
     1861 cagcccagct aagaccaaag atgcactggc ccttaaatcc cctgccatag ttggtgcacc
     1921 gctaaggtaa ggcactcctc ttacaacggc gcagtttcca acaagcttta tgtccgcacc
     1981 catccttctt agttcgcctg catgcatgaa tctctgaggg aatatcgttt cagtgataat
     2041 gctaaggcca tcggcaacgc tcataagagt catcatctgc gcctgcatat ccgtaggaaa
     2101 tccgggatat gggagggttg taatgtcaat gctctttatt ttatcgcttc cccgaacttt
     2161 tataccgctg tctatcctag atacttcagc tcctacttcg agtagcttac cgattaaggc
     2221 gcctgaattt tcaaacctgc atttctttat taggatatca ccttgagtta atgcagaggc
     2281 gattaaaaag gttccagcct cgatcctatc aggcataacc tcgtgtccat ttataggtga
     2341 aagcttatcc acccctcgta ttgtaataat atctgttcca acaccctcga tttccgcacc
     2401 cattttctta agaacgtgta cgagttctgt gacctctggc tcgcatgcgg cgttaagaag
     2461 catagtttcg ccctgtgcga gtacagaggc aagcacaatg ttttctgtac ctgtgacagt
     2521 tgagatatca aacgggatta cgcttccatt tagtttaggt gatgtagctt caatgtagcc
     2581 ctcttcgatt ttgatatccg caccaaggat cccaaggccc ttgaggtgtt gatcaattgg
     2641 tctttctcca attgcacatc ctccgggaag cgatactcga gcgtggccga atcttgccac
     2701 taggggaccg agaacaagta ctgaagctcg catggtttta acaagttcat acggggctac
     2761 ccaattattt aaacgagttg tgtcaattga caaagtacct ttttcgtatt cgacctcagc
     2821 gcctaagatt ctaagtagct ttgacattgt cttaacatca tctaattcag gtacgtttgt
     2881 aaatgtgctt ctgccttccg taagaatgca ggacgcaata agaggtagca cggcgttttt
     2941 agcaccgctt atggtaacct caccttctaa tctttttcca ccttcgacta ttatcttttc
     3001 cattttccct taaccaccct ttctatatct gccaggtctt ttacagtaga aacttcttta
     3061 aaaccaatgc actcgaaagt ctctaacacc ttttccgatt gattggctcc aatttcaata
     3121 atacaccatc ccccattttt caaaactctt cctgcttcat gaacgatctt ctttatacag
     3181 tttaatcctt cttcgcctga agttgaccgt gagtcttcga caagctcaga ccgaacggta
     3241 gaagggtgga gtcgaagggc ctgtcctgag ccatgccgaa ggatcgagcc acctccaata
     3301 agtgatattt taggttcaaa atctcttata tcggattcga gtctatgaaa ttcactttca
     3361 ggtatgtaag gtggatttga aacaattata tcaaaggaat ttcccttgaa acagcttaaa
     3421 aaatctgcac aaatgaacga aattctttct gcgaccttat gtcttaccga atttgctctt
     3481 gcaaccataa gtgcttcaag tgatatatca gcagcaaaga tttgtgcatt ttcagattcg
     3541 caaccaatgg tcactgctat gcatccgctg cctgtaccga catcaagaat aagagaatta
     3601 ggagtagttt tagttatttt taaagcttct tctactaata tttctgtttc aggtcttggt
     3661 atgagaacct gaggggttac aataaacgac cttgagtaaa actctttttc gccaagaatg
     3721 taagcgatag gttctctttt tattcttctt tcaagcagta attcgagctc tttaatctta
     3781 tcagattgga cccaattttt aggatgggcg taaatgtaag ttgtatttat tctaagaacc
     3841 ttagaaagca gcagcgccgc ttcaaactca gggttttcta cgcctgattt tttcagtaag
     3901 tctcttcctt taaggtaaag ctctttcaaa gatacccctt ctccattctt cattatttgc
     3961 tgtaaaaaat cttatttagt ttacctggta ttttcaaatt ggagagtttg aaagatttgc
     4021 ctgtctgcat actatgttat aatcacttgc tcgggtttcc ttaatgtcct attccatgct
     4081 ccgagcgagg gacatatcac gaacaagttc gtgcctacaa tctttttgtg acctattgtg
     4141 tcgaattgta tatataaact aattgttggc acagactagt ctatgctatt taactgtctg
     4201 ccagcaagac tggcaggcta cccatttatt gtattggatt atgagagaca tttccttcga
     4261 cacgctcagg acaggcttgt ctcctcgtgt agggttagaa aacctcaact atcgcagcat
     4321 tcccaaattg caaattgtca aaccacctgt agggcgaacc ttgcctgtgg cgagccgagt
     4381 cgagccgtgt tcgcccatca tattagggca gacacaccgg tctgccccta cattgaaacc
     4441 ttaacgacga tggtattact ttttagtatg ttatatttta acaatttgat tatgaactcg
     4501 tattaagagg tataggaata tgattggaaa gggattaaga ctgactaaga tttttggaat
     4561 agaaatctgg gttgattatt catggttaat aatttttgcc cttgttgcgt ggagcttggc
     4621 ttctagttat tttccacatg agtatccggg actaggagtt acaccttatt ggattatggg
     4681 ggct
//
LOCUS       DATDRL010000215         9324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2645575, whole genome shotgun sequence.
ACCESSION   DATDRL010000215 DATDRL010000000
VERSION     DATDRL010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9324
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2645575"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VI935_07365"
     CDS             complement(<1..924)
                     /locus_tag="VI935_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HLE25454.1"
                     /translation="MKPFGFPPKQGLYDPQFEHDSCGIGFVVNTKGKKSNDIVRHALN
                     VLKNLYHRGACGCEANTGDGAGILMQIPHAFFRKVCSEIGIHLPSSGEYGIGMVFLPP
                     DPYDRRECEKRLEDIIIEEGQYLLGWRTVPTNNASIGNTARSCEPCVRQVFIGRNSRI
                     KDDMAFELKLYVIRKRAERDIRYSGMKGGEYFYFPSLSYKTIVYKGMLTALQVEGFYP
                     DLMDSDIETSLALIHSRFSTNTFPSWGRSHPYRYLIHNGEINTLRGNINWMHTRQSMF
                     ASDLFRDDLPKILPIINPDGSDSAMFDNCLEF"
     gene            complement(1055..1597)
                     /gene="cysC"
                     /locus_tag="VI935_07370"
     CDS             complement(1055..1597)
                     /gene="cysC"
                     /locus_tag="VI935_07370"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HLE25455.1"
                     /translation="MKGFTVWFTGLPSSGKSTLARMLEKVLNDMDFHVEVLDGDEIRL
                     RLSKGLGFSKEDRDENIRRISYVANIVTRCGSIAITCAISPYREIRNEARKEIGRFIE
                     VYVKCPLEECIKRDLKGLYKKALDGEIKNFTGISDPYEEPLNPEVIVETHNETHEESL
                     SKIVSGLIELGYITKEKLRM"
     gene            complement(1691..2725)
                     /gene="tsaD"
                     /locus_tag="VI935_07375"
     CDS             complement(1691..2725)
                     /gene="tsaD"
                     /locus_tag="VI935_07375"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HLE25456.1"
                     /translation="MLSLILSIESSCDETAAAVIKDGKTVLSNIVASQIEIHKEFGGV
                     VPELASRKHVETIIPVIEEALTKSNEELTDIEGIAVTNGPGLIGSLMVGISTAKAISY
                     ALGIPFIGVDHLEAHISAVHLEYEVDFPFLGLIVSGGHTSLYLVTGYTDFKLLGKTRD
                     DAAGEAFDKAAKFLGLGYPGGVEIDRISKEGNSTAISFPRPFITSSSFDFSFSGIKTA
                     LVYYLKKNPNLNEEHLKNICASYQEAIVETLVEKTVKAALSSGVMSVVIAGGVACNSR
                     LRELGEKRFEEEGISLFIPSPKYCTDNAAMIGALGFYKLNEGDYSELTLNPYSTSRPK
                     YIRGRGLIAN"
     gene            complement(2781..3245)
                     /locus_tag="VI935_07380"
     CDS             complement(2781..3245)
                     /locus_tag="VI935_07380"
                     /inference="COORDINATES: protein motif:HMM:NF037830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25457.1"
                     /translation="MTVRKKKSKKRFDASSGEKLPDPLLFFLDRAIGKRFVSEALSSP
                     DLNIKVEVLDDYFEPNTTDEEWLKSAGKKGWIVFTKDKKIRYRATVVEIIKKGKIRMF
                     ILSRGNLSGHEMARIIINALPVIKKSILKHHPPFIVSITRSANLLPIELSNV"
     gene            complement(3181..3846)
                     /locus_tag="VI935_07385"
     CDS             complement(3181..3846)
                     /locus_tag="VI935_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009631756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HLE25458.1"
                     /translation="MKKDIRYTPCYSVSEAAFYLRLPVSTLRAWVGRQRGFEPLIAIA
                     QDKPPSLSFVNLVEAYVLAAIRRKCIISMNKVRRAIEYIIEKFHSEHPLADKEFETDG
                     LHLFVREYGTLINITQEGQIELEEIVKQYLKRIGRDTKGLPVSLYPFSRTGEPDEPRL
                     IIIDPQIAFGRPVLATKSIPVEVIAERYRAGESIADLTYDYDCTQKEIEEAIRCELWR
                     EAA"
     gene            4034..5848
                     /gene="thiC"
                     /locus_tag="VI935_07390"
     CDS             4034..5848
                     /gene="thiC"
                     /locus_tag="VI935_07390"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HLE25459.1"
                     /translation="MTKKDKIPAQNLISRDPYPRSTRIYVNGKIHDIRVAMREIELDD
                     SDPEFSTNGSSTKNNPITVYDTSGPYTDPDVEIDINKGLSRIRGKWILDRGDVEKLED
                     FSSEFAKKSLRDPKLDEIRFKHIKKPLRAKAGKTVTQLHYARRGIVTPEIEYIAIREN
                     QRIDVLREQMKDLAIYHKGESFEANIPDTYITPEFVRQEVARGRAIIPSNINHPESEP
                     MIIGRNFLVKINANIGNSAVTSSIEEEVEKAVWACRWGADTIMDLSTGKNIHETREWI
                     IRNSPVPVGTVPIYQAIEKVGGKAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYI
                     PLTAKRMTGIVSRGGSIMAKWCLAHRKESFLYTHFEEICEIMKAYDVAFSLGDGLRPG
                     SIADANDEAQFAELETLGELTKIAWKHDVQVMIEGPGHIPMHLIKENVDKQLEICHEA
                     PFYTLGPLTTDIAPGYDHVTSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKKDVKEGVI
                     AYKIAAHAADLAKGHPAAQLRDNVLSKARFEFRWSDQFNLSLDPDASLAMHDETLQDE
                     YFKEAPFCSMCGPKFCSMRITHDIRKMASEIMEEIEAM"
     gene            5933..6742
                     /locus_tag="VI935_07395"
     CDS             5933..6742
                     /locus_tag="VI935_07395"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLE25460.1"
                     /translation="MHVHFFPPGVFQAIWRYFETPSNGLWNIKYKLYGGKLVQTLKME
                     GVKRFTSLLYAHKPGLAQYLNDFVKESADHFPELIPFGTVFAGDGNSQRVARQIFEEY
                     KFFGVKLHPFVSNENLDDPRFFPVYEIMQDYGKVLICHPSSVPVYLKTDGANRLRNIL
                     IRFPRLKVIVAHCGAFEYGDYCVLTDDFEYIYFDTAMNCVHTKALTNNCPGREFFLKN
                     QDRILFGSDFPNIPHAYSNQVMALKKLNLGETVERKIFNDNAPNLLGLKEK"
     gene            complement(7274..8434)
                     /locus_tag="VI935_07400"
     CDS             complement(7274..8434)
                     /locus_tag="VI935_07400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLE25461.1"
                     /translation="MDNSKKIFYVLFIASIVTNIGSGMIASLLGVYSKTLGATGLQLG
                     IIYSGISFSRAILSPPIGKLSDEKGRKAFLIFGLLAYTLISFGYIYAETPWGLIIVGL
                     LHGAAAAFVLPVAFAYIGDITPIGKEGEYLGTFGISLFIGWAIGPILGGVIADVLGYK
                     EAFLGMGILSFASLVITFFMLPESETIKKDGNSISYKSILKDRLLVVVLSVRFLHLIT
                     ISSIVTFVPIFALSINLSLSQIGTILMLNILVMGLLQHPFGKLADRANRINMLIIGGI
                     IASAGLMGIPFSTNFLQLLFWNMVMAIGSALSFPTTNAIMTVKGRTTGMASVMAVFNF
                     AMSAGMALGPLIAGVIYDQMGLSWVFVVLSVISTLGLLLLYPFMRKSPDLSF"
     gene            9007..>9324
                     /locus_tag="VI935_07405"
     CDS             9007..>9324
                     /locus_tag="VI935_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25462.1"
                     /translation="MDFESIAREVTSRSKITVTQKDLERLFGALKKTKLPWELIDLSD
                     FPVPSVFETMKVLHEKGLVLISDRGFKLTRKGKEVANEVHPVEDISCSMCDGRGINLE
                     GFSQ"
BASE COUNT         2887 a         1825 c         1871 g         2741 t
ORIGIN      
        1 aaactccaag cagttatcga acatagccga gtcacttccg tctggattaa tgatgggaag
       61 aatcttaggt aagtcatccc tgaagagatc agatgcaaac atggactgcc gtgtatgcat
      121 ccagttgatg tttcctcgta gtgtatttat ctccccgttg tggatcaaat aacgataagg
      181 atgggagcgt ccccaactgg ggaacgtatt tgtgctaaat cgcgaatgga taagtgcaag
      241 tgaagtttct atatcactat ccatgaggtc tggatagaat ccctcaactt ggagagctgt
      301 gagcataccc ttgtaaacga ttgttttata ggagaggctt ggaaaataaa agtattcacc
      361 acccttcatt cctgagtaac gaatatccct ttcagcacgc ttgcggatga cataaagttt
      421 tagctcgaaa gccatgtcgt ccttaatcct tgaattacgt ccgatgaaaa cctgtctcac
      481 gcatggttca cacgacctgg cagtatttcc tatagatgca ttattagtgg ggacagtacg
      541 ccatccgaga agatattggc cttcctctat tataatatct tctaaacgct tttcgcattc
      601 acgtctgtcg tatggatcgg gagggagaaa gaccataccg ataccatatt ctcctgatga
      661 aggtagatgg atgcctattt cagaacagac tttcctgaaa aatgcatgtg gaatctgcat
      721 gaggatacct gcgccatcac ccgtgttggc ttcgcaacca caagcacccc ggtgataaag
      781 gttcttaagt acgttaagtg catgacgcac aatatcattt gactttttgc ctttagtatt
      841 aacaacgaat ccaattccac aggagtcgtg ttcgaattga gggtcatata ggccttgttt
      901 agggggaaat ccaaagggct tcatgataca acttttcctc gctcccttaa tacttattgt
      961 ctaaaatact acaccgaact tacaaataac tcaaatttat agttatatat ttttaagaaa
     1021 aattttcctg agcttaacga aataatctac aaacttacat cctcagcttt tctttggtaa
     1081 tgtatcccaa ctcaattaaa ccgctaacaa ttttacttaa actttcttca tgcgtttcat
     1141 tgtgtgtttc aactattact tcaggattta gtggttcttc ataggggtct gagatgcctg
     1201 taaaattttt aatctcacca tctagagctt ttttatataa tcccttcaaa tcccttttta
     1261 tacattcttc taaagggcac ttcacgtaaa cctcaataaa tcttccgatc tctttacgtg
     1321 cctcatttct aatctcacgg taaggagata tggcacaagt aattgcaatg gatccacacc
     1381 ttgtgacaat gtttgctaca tatgatattc ggcgtatatt ttcgtctcta tcttctttag
     1441 agaatcctaa acccttagat agtcttaacc ttatttcatc gccgtctagc acctctacat
     1501 gaaaatccat atcgttaagt accttctcga gcatccttgc caatgtgctc tttccagaac
     1561 taggtagtcc agtaaaccaa acagtaaaac ccttcatttt ttctccttaa ttttcccttc
     1621 gtaataaacg tgtctttcag ttaataaaat aaggcgcatt tattaatttg gtatgctatg
     1681 ttaaaacctg ttaatttgca attaatccgc gccctcttat gtactttggt cgagatgtgg
     1741 agtaaggatt taaagtgagc tctgaataat caccctcatt aagcttgtaa aaccctagcg
     1801 cgccaatcat tgcggcgtta tctgtgcagt atttaggcga aggaataaaa agggaaattc
     1861 cttcttcttc aaatctcttt tccccaagct ctcttagacg tgaattacac gcaactccgc
     1921 ctgcgataac aacagacata actccgcttg atagcgcagc ttttacagtt ttctccacta
     1981 gtgtctcaac aatcgcttct tgataacttg cgcaaatatt ctttaaatgt tcttcattta
     2041 aattaggatt cttcttcaag taataaacca atgctgtttt gattccacta aagctaaagt
     2101 caaatgaaga agatgtaata aatgggcgtg gaaaagaaat cgcggtggaa tttccttctt
     2161 ttgaaattct atcaatttct acacctcctg gatagcccaa tccaagaaac ttagcagcct
     2221 tatcgaatgc ttctcctgcc gcatcgtctc ttgtcttacc aaggagtttg aaatcagtgt
     2281 aaccggttac taaataaaga cttgtgtgtc ctcctgaaac tattagcccc agaaaaggaa
     2341 aatctacttc atattcaaga tgaacggctg agatatgcgc ttcaagatga tcaaccccta
     2401 taaagggaat cccaagggcg tacgagattg cttttgcggt agaaattcca accataagtg
     2461 agccaattag accaggtccg ttggttaccg cgattccttc aatgtcagta agttcttcat
     2521 ttgacttagt gagagcttct tcaattactg gaattattgt ctcgacatgt tttcttgatg
     2581 caagctcagg tactactcca ccaaattctt tgtgaatttc tatttgagaa gctactatat
     2641 ttgagagaac ggttttccca tctttaatca cagcggctgc cgtctcatca cacgatgatt
     2701 ctatacttag tattaaagat agcataacaa tttattttac aggatagttt aatattcaca
     2761 aataaagttg atttcaatct tcaaacattg ctcagttcta ttggtaataa gtttgcagat
     2821 ctagttatgc taacaataaa gggtggatga tgttttaaaa tagacttttt gattacagga
     2881 agtgcattga taattattcg ggccatttca tgacctgata aattccccct acttaatata
     2941 aacattctga tttttccttt ttttatgatt tctactacag tggcacgata acggatcttc
     3001 ttatctttag taaatacaat ccaacccttt ttaccagcag attttaacca ttcttcatcg
     3061 gtggtattag gttcaaaata atcatctaga acttccacct taatattcag atcaggactt
     3121 gaaagagctt cagatacaaa tctttttcct atagctctgt caagaaagaa taaaagtgga
     3181 tcaggcagct tctcgccaga gctcgcatcg aatcgcttct tcgatttctt tttgcgtaca
     3241 gtcatagtcg tacgttaaat cagctattga ttccccggcc ctatagcgtt cagctataac
     3301 ttcgaccgga atgcttttag ttgccaagac tggtctacca aatgcgattt gaggatcaat
     3361 aatgattaat ctaggttcgt caggctcacc agttcttgag aaggggtata ggcttacagg
     3421 taggccttta gtgtcacgtc cgatgcgttt taaatattgt tttacaattt cttcgagttc
     3481 aatctgccct tcttgtgtaa tattgattaa agtgccgtat tcacgtacaa agaggtgtaa
     3541 tccatcggtt tcaaattcct tatccgctaa gggatgttct gaatggaatt tttcaattat
     3601 atattcaata gctcgcctta ctttattcat tgatataatg cattttctac gaatagcagc
     3661 aagcacatag gcttcgacaa gattcacgaa cgaaagtgaa gggggcttat cttgagcaat
     3721 agcaatcaat ggctcaaaac cacgttgtct acctacccat gcgcgaaggg ttgatacagg
     3781 taatcttaaa taaaaagctg cttcgctcac actataacag ggtgtataac ggatatcttt
     3841 tttcattttc cacatcccta accctatttt ctttaaacat aagtaactaa taagattgtg
     3901 aataccaaaa aattttttgt caagatttgg tataataagg aggataatcc cgcaaagaaa
     3961 acatatgatg tgttctgagt aatataatag ttcatagttt gacacataaa tcaaatcctt
     4021 atatcataaa accatgacaa agaaggataa aattccagca caaaatctta tcagccgaga
     4081 cccttatcca cgttctacga ggatatatgt gaatgggaaa atacatgata tcagagtcgc
     4141 aatgcgtgaa attgagttgg acgattctga tccagagttt tctacaaatg gcagctcaac
     4201 taaaaacaat ccaattacgg tttacgatac cagcggtcct tataccgatc ctgatgtaga
     4261 gattgatatt aataagggtt tatcccgtat tcggggaaaa tggattctgg atagaggtga
     4321 cgttgaaaaa ctagaggatt tttcatccga atttgcaaaa aaaagtctaa gagatccaaa
     4381 gctcgatgaa attcgattta aacacataaa aaaacccctt agagctaaag caggcaaaac
     4441 tgttacccaa ttgcattatg cccgaagagg catcgttact cccgagattg aatatatagc
     4501 catacgtgaa aaccagagaa tagatgtatt aagggaacag atgaaggatt tggccatata
     4561 tcataaagga gagagttttg aagccaatat accggatacc tacataactc cagaattcgt
     4621 tagacaagag gtcgctaggg gaagagccat catacccagt aatatcaatc atcccgaaag
     4681 cgagccaatg ataatcggta ggaacttttt ggtaaaaatt aatgccaaca ttggtaactc
     4741 ggctgtgact tcaagtattg aggaggaggt agagaaagcc gtttgggcct gccgctgggg
     4801 tgcagacaca ataatggatt tatcaaccgg taagaatatt cacgaaacaa gagaatggat
     4861 tattagaaat tctcctgttc cggtgggtac agttcctatt tatcaagcta tagaaaaggt
     4921 aggtgggaag gcggaggatt taacatggga ggtatataga gacaccctaa ttgagcaggc
     4981 agaacaagga gtagattact ttaccattca tgcaggagtg ctcctaagat atattccttt
     5041 aacagcaaaa aggatgacag gaattgtttc aaggggcgga tctattatgg caaaatggtg
     5101 tttggcgcat cgcaaggaga gcttcttata tacccatttt gaggaaatat gcgaaatcat
     5161 gaaggcatat gatgtggcat tctcattagg cgatggcttg cggcccggct ctattgctga
     5221 tgcaaacgat gaggctcaat tcgcagagct agaaacctta ggagaattaa ccaagatcgc
     5281 ttggaaacac gatgtacagg tcatgattga agggcctggg catattccca tgcatttgat
     5341 taaggagaat gtggataagc aactcgaaat ttgccatgag gctccttttt atactctggg
     5401 tccgttaacg accgacatag cccctggtta tgaccatgtc acctctgcta ttggagcagc
     5461 tatgataggc tggtacggca cggctatgct ttgttatgta acgccaaaag agcacttagg
     5521 attgcccaat aagaaggatg tgaaggaagg ggtgatcgct tataaaatag cagcacatgc
     5581 tgcagacctg gctaaggggc atcctgctgc acagcttagg gataatgttt taagtaaggc
     5641 acgatttgaa ttcaggtgga gcgatcaatt taatctctct ttagaccctg atgcttcact
     5701 ggcaatgcat gatgaaaccc tccaggatga gtactttaaa gaggctccgt tttgctcaat
     5761 gtgcggtccc aaattctgtt ctatgagaat aactcatgat ataagaaaga tggcttcgga
     5821 aataatggaa gaaatagaag ctatgtgaac ttagttaaga ctacctagtc tatcgctttt
     5881 cttaaaaacc atcactcata attatgcccc cataaaaacg accatgattg atatgcatgt
     5941 acattttttt cctccgggag tatttcaggc gatatggaga tactttgaga ctccgagtaa
     6001 tggtttatgg aatatcaaat ataaattgta tggtgggaag cttgtacaaa ccttgaaaat
     6061 ggagggtgta aagcgtttta ccagccttct ctatgcgcat aaaccaggcc tagctcaata
     6121 tctaaatgat ttcgtcaaag agtcagcgga tcatttccct gagctgattc ctttcggaac
     6181 tgttttcgct ggagatggta attcccaaag ggttgcaagg caaatctttg aagagtataa
     6241 attttttggg gtaaaacttc acccctttgt ttcaaacgag aatttagatg atccacgttt
     6301 tttccctgtc tatgaaataa tgcaggatta tggaaaggtt ctcatttgtc atccgagttc
     6361 agtaccagtt tatctaaaga ccgatggagc gaatcggtta agaaacatct taatcagatt
     6421 cccccgtttg aaggtaattg ttgcccattg cggcgctttt gaatatggag attattgtgt
     6481 gctaaccgat gacttcgaat acatttattt tgatactgct atgaattgcg ttcacaccaa
     6541 agcccttaca aataactgtc caggaagaga attctttctt aaaaatcaag atagaatact
     6601 tttcgggtca gattttccta acatcccgca tgcatattcg aatcaagtta tggctttgaa
     6661 aaaattaaat ctaggtgaga ctgtagaaag aaaaatattc aatgataatg cgcctaactt
     6721 actcgggtta aaagagaaat aaaggatcgt gattcgtgaa tcgtgctttt tgtacagtgt
     6781 accgaacttc gaatcccgag gcccgagcta cgaatggtcg aagataatag gcttgtcgtg
     6841 ccaaaaatag cacagacaag tctgtgccta caaatacaaa ggttgtaggc acgaatttat
     6901 tcatgcaact taatttataa ccgtcgaagc ataatctccg gagaggggaa aatggctcaa
     6961 acagagcatc taccaattta caagagtgca tatgacctgt gtctctattt tgagcaggtg
     7021 gcgagaaatt tctcacgtta ccacactaat tccatcatcc cccatcagcc ggactggctg
     7081 gctgccccct atcaggggac gataggggat cttcctgtcc cattctatag aaagaaagat
     7141 attcaacgac tatccgatta aattgctagg attggaaagg caataaccga gcgtgatctg
     7201 tggctagtga atcgtgattt gtgtatcgtg catcgaacca cgaatcacga ggcccgaact
     7261 acgaacaacg attttagaat gataaatctg ggctttttcg cataaaaggg tagagtaaaa
     7321 gcaaacctag agttgatata acacttaaga ctacaaaaac ccacgaaagt cccatttgat
     7381 catatataac tccagcaatt agtgggccga gagccatccc ggcactcata gcaaaattaa
     7441 aaaccgccat gacgctcgcc atacctgtag ttcttccttt cacagtcata atggcattag
     7501 tagttggaaa agaaagggca cttcctatag ccataaccat gttccaaaat agaagctgca
     7561 aaaagttggt tgaaaatggg attcccataa gccctgctga agcaattatc ccaccgatta
     7621 ttagcatgtt tattctgttt gctctgtcag ctagttttcc aaatgggtgt tgaagcagtc
     7681 ccataacaag aatgttcaac ataagtatcg taccgatttg ggaaagactc aaatttattg
     7741 acaaggcaaa aataggaacg aatgttacga ttgaagagat tgtaattagg tgaagaaacc
     7801 taactgataa aactacgact agaagcctgt cttttaaaat agatttgtat gaaattgagt
     7861 taccatcctt ctttatagtc tcactttcag gaagcataaa aaatgttata acaagtgatg
     7921 caaaactaag aatccccatg ccaaggaaag cctctttgta acctaggaca tctgcaatga
     7981 caccccctaa gataggaccg atagcccatc ctataaaaag cgaaatgccg aatgtaccta
     8041 agtattcgcc ttcctttcct ataggagtta tatcccctat atatgcaaaa gctacaggta
     8101 ggacaaatgc agcagcggct ccgtgaagaa gcccgactat tattagcccc cagggtgttt
     8161 ctgcatatat ataaccaaac gaaatcagtg tgtatgctag aagcccaaaa atcaaaaaag
     8221 cttttctacc cttctcgtct gacaactttc caatcggggg ggacaggata gcccttgaaa
     8281 aggagatccc agagtagatg attccaagtt gaagaccggt ggctccaagg gtctttgaat
     8341 acacaccaag aagtgaggct atcattccgc ttcctatatt cgtaacgatt gaagcgataa
     8401 atagtacata aaatatcttt tttgaattat ccatttgaga ggcaggaaat ataacatagt
     8461 taaggtaggt agggaaatta ttgaaagaac attaaattga gctaagcgcg tttgaatttg
     8521 tgatgttaga ataaagatta gtaagtttta gttcgaaatc cagaaacagc aactataggg
     8581 agaagatatt cattttatca ggagcgaacg gaatgaacga gtgtgatgct tatggtcagc
     8641 atactgtgaa ccggaatatg gtcaatacaa agggaaaatg tcacctatat gtagcggaag
     8701 gctttagcct tacatatttt tctctatggc gacctaaagg tcgcagctac attatttcta
     8761 taaaaacggt agtggagaac tttagacctc tacaaatgtc attcccgcat gttacccgct
     8821 taaaacatgc gggcacaagt taaagcggga atcaatatag cttcggtgtt catggatgcc
     8881 cgattaatgc actcgggcat gacatgtaca tggcgcggaa tgagtctaga acttgctagc
     8941 atcgtttgaa agtgttcaaa ttttttggta taaaattttg atttagtatt agggagagta
     9001 ggtataatgg atttcgagag tattgcaaga gaggttacat ctaggtctaa gataactgtt
     9061 acccagaaag atctagaaag gctttttggt gctcttaaga aaaccaagtt gccttgggaa
     9121 ttaattgacc tgtccgattt tccggttcca tcggtttttg aaactatgaa ggttctacat
     9181 gaaaagggtt tagtcttaat ttcagatagg gggtttaaac tcacgagaaa aggaaaagag
     9241 gtagcaaatg aagtgcatcc ggtagaggac atttcttgct caatgtgtga cggtagggga
     9301 attaatttag agggattttc tcaa
//
LOCUS       DATDRL010000216         4639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_452855, whole genome shotgun sequence.
ACCESSION   DATDRL010000216 DATDRL010000000
VERSION     DATDRL010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452855"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VI935_07410"
     CDS             complement(<1..871)
                     /locus_tag="VI935_07410"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HLE25463.1"
                     /translation="MKRAYKVFWVSLFIISLTISIQEALAQSEDIAGEKQEEEKPQNP
                     TLRTIKYKVRVGDTLYSVAKKFRVSVDELKKLNSLKDDNLGVDQVISVPYDFKRSKTL
                     STSGDETSTSKKQETEATTLEEENKEIGAPTNDLETKAPEVQATAQEKLQVGEEVIKE
                     KEVKAPGGSPKQESIPEEEVVNLQTEMDIRDLIQTVSEITGETFLLEESVKGKKVTVV
                     SPKGGFKKQNAFRLFEAILDLNGFALVKKDGVSKIIPKRDIKIESLPTGSGIEYGSPS
                     DMYVTRLVPLKNIN"
     gene            complement(1027..1977)
                     /gene="gspC"
                     /locus_tag="VI935_07415"
     CDS             complement(1027..1977)
                     /gene="gspC"
                     /locus_tag="VI935_07415"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HLE25464.1"
                     /translation="MIASVIKKYIWVINLILLAGIAYTTVKIVTDKIKEAVTPPVSEA
                     FASSKLSPENRLDGNLRHSSPRSSYNVILKRNIFNLKRTSLGSSGLSVNEVPQTTLKL
                     ELLGTVLGGSGKSAIIKNLDTGKVRGYVEGELIDIVISESVRLARIENCIAMVERGEG
                     PETIKCKKDIGGTLSVVSQSQPGQIGEANRIERRDKSSREKTQTEAENLDVREVSEGV
                     YEVDQKMLEEALSDPNQLMTQARAIPQDDGLKFFAIRPNSLFFKIGLRNGDVLHSIND
                     IGLDNIENAFGFFEELRGQTHFSIDLSRRGQKFTYEYSIK"
     gene            complement(2052..3122)
                     /gene="galT"
                     /locus_tag="VI935_07420"
     CDS             complement(2052..3122)
                     /gene="galT"
                     /locus_tag="VI935_07420"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HLE25465.1"
                     /translation="MRELRFDPIRNRWVIISSERARRPSDYTIAGVGAHTDGKSLAAC
                     PFCEGSEEKTPLEIFAIRSHGTKPNSPGWRVRVVPNKFPALQIEDDAIRYGTGIFDVV
                     SGAGAHEVIIETPDHNKELGDLDTDWIKDVLFAYRQRIRDLFGDHRLRYVLIFKNHRS
                     EAGASLTHTHSQLIATPMVPPVVKQELTACRENYRAKERCLICDLIKQEIDFGARIIY
                     ETRDYIIWSPFASSFPFETWILPKHHLHDYSMLGDNDLRELAPVLKLLLNSIKGLLND
                     PPYNFVLHTSPPRFKRPGQPQHWDSIESDFHWHIELIPRLTTIAGFEWGAGFFINPTP
                     PEAAADYMRQDITRLIEVRKVI"
     gene            3674..>4639
                     /locus_tag="VI935_07425"
     CDS             3674..>4639
                     /locus_tag="VI935_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLE25466.1"
                     /translation="MNAESPITDHDIFLFKQGSHFKLYNKLGSHPITVNETQGTYFAV
                     WAPNAERVSVIGDFNGWNPESHPLGVRWDGSGVWEGFIPYLEKGATYKYQITSKHNNY
                     NVEKADPYAFFSESPPKTASIIWDLNYDWKDDEWMKNRFKFNSLDSPFSIYEVHLGSW
                     RRVPEEGNRFLTYREIAHCLADYVKYMGFTHVEFLPVMEHPFYGSWGYQTSGYFAPTS
                     RYGTPQDFMYLVDYLHQNGIGVILDWVPSHFPDDKHGLIYFDGTHLYEHADPRRGFHP
                     EWNSYIFNHGRNEVRAFLISNALFWLDKYHIDGLRVDAVASMLYLD"
BASE COUNT         1318 a          987 c          794 g         1540 t
ORIGIN      
        1 catttatatt tttcagtgga acaagcctcg taacatacat atccgaggga ctaccgtatt
       61 cgataccaga acccgttggt aggctttcaa tcttaatatc cctttttgga ataattttgc
      121 taactccatc ttttttaacg agagcaaacc cgtttaaatc taagatggcc tcaaagagtc
      181 taaaggcgtt ttgtttttta aatcctccct taggtgatac tacagtcact ttcttaccct
      241 ttacagattc ctctagtaga aatgtctccc cagtgatctc acttaccgtt tggattaggt
      301 ctcttatatc catttctgtc tgaaggttaa caacttcttc ctcaggtatg ctctcctgtt
      361 ttggagatcc ccccggtgct tttacctctt tctctttaat aacctcttct cccacttgta
      421 gcttttcttg agctgtagct tgcacctcag gtgctttagt ttctaaatca tttgttggag
      481 ccccaatttc cttgttttct tcttctaaag tagttgcctc agtctcttgt ttcttagatg
      541 ttgatgtttc atcaccactt gtcgatagag ttttagaacg tttgaaatca taaggcacac
      601 ttataacttg atccacccct aggttgtcat cttttaggct gttcaatttt ttcaattcat
      661 caaccgagac tcggaacttc ttagccacgc tatatagggt gtctccaacc cttaccttat
      721 attttatggt tctaagagtc ggattttgtg gcttttcctc ttcttgcttc tctcctgcaa
      781 tatcctctga ttgagctaag gcctcttgaa tacttatagt caaagaaata ataaacagag
      841 aaacccaaaa aaccttgtag gctcttttca ttccctgtgc tccccgaatc ttaaacttat
      901 gctgacaacc ttttctatat tcataatttc tcttcgctat gaaacataga cgcattcatt
      961 tatgcatcta ctatttatta gaattatttt tgttgattca tcctcatgtt acgattttta
     1021 attatgctat ttaattgagt attcatatgt gaacttctgt ccacgcctcg atagatctat
     1081 actaaagtga gtttgtcctc tcaattcctc aaagaagccg aaagcattct caatgttatc
     1141 aagcccaatg tcgtttatgc tgtgaagaac atccccgttt ctaagtccaa ttttaaagaa
     1201 aagactattt ggccttattg caaaaaactt tagcccatca tcttggggaa tcgcacgagc
     1261 ctgagtcatt agttgatttg ggtcgcttag agcctcttca agcatcttct ggtcaacctc
     1321 ataaacccct tctgacactt cccttacatc caaattttca gcctccgttt gagttttctc
     1381 cctagaactc ttatccctac gttctatacg attcgcttcg ccaatttgtc caggttgaga
     1441 ctgagaaaca accgataatg tacctccaat atccttttta catttgatcg tctcaggccc
     1501 ttcgcccctt tcaaccatag ctatgcagtt ttcaatcctg gctaacctaa cactttcaga
     1561 aataactata tcaatcaatt ccccttcaac ataaccccta accttaccag tatctagatt
     1621 ttttataatc gctgattttc ccgaaccccc aagaacagtt ccaagcagtt caagtttgag
     1681 tgtagtttga gggacctcat ttacacttaa ccccgatgat cctaatgatg tgcgctttag
     1741 attaaatata ttccttttta aaatcacatt atatgaactc cttggagacg aatgccttaa
     1801 attaccatca aggcggtttt ctggagataa ctttgaagat gcaaaagcct ctgatacggg
     1861 tggggttact gcttctttaa tcttatcagt tactattttc acagttgtat aggcaatccc
     1921 cgctaaaagt ataagattta tgacccagat gtactttttt ataaccgatg caatcattta
     1981 agtaaataag ttaatttatg ctaacaaaat aaagcttttt actttatatg tcaataggga
     2041 ttttcttgta atcatataac ctttctgacc tcaataagcc tagtaatatc ttgcctcatg
     2101 taatctgctg ccgcctctgg aggggtcggg tttataaaga aaccagcccc ccattcaaac
     2161 cctgcaatgg ttgtaaggcg tggaataagc tctatatgcc aatgaaaatc ggattcaatt
     2221 gagtcccaat gctgtggttg gccaggacgt ttgaatcttg gtggcgaggt atgaagtaca
     2281 aaattgtaag gcgggtcatt taaaagcccc tttatggaat taagtaatag ttttaataca
     2341 ggtgctagtt ctctaagatc attatcacct agcattgagt aatcatgtaa atggtgttta
     2401 ggaagtatcc atgtttcaaa aggaaagctt gaagcaaacg ggctccagat gatatagtcc
     2461 ctagtctcat aaatgatcct tgcaccaaaa tcgatttctt gtttaattag atcgcagatg
     2521 agacatcttt cttttgctct atagttttct ctgcatgcag taagctcctg cttaactact
     2581 ggtggaacca tgggggttgc tattagttga gagtgagtat gggtaagaga agcgccagct
     2641 tcagacctgt ggtttttaaa tatcaagaca taccgtagcc tatggtcacc aaaaaggtcc
     2701 cttatccttt gtctataagc aaaaagcaca tcttttatcc aatctgtatc taagtcacct
     2761 agttccttat tatgatcagg ggtctcaata ataacctcgt gcgcaccagc accgctaact
     2821 acatcgaata tacccgtacc gtatctaata gcatcgtctt ctatctgtag agctggaaat
     2881 ttattcggaa caacccttac tctccatcca ggtgagttag gctttgtccc atgacttctt
     2941 atagcaaata tttccaaagg tgttttctct tccgatccct cacaaaatgg acaggctgct
     3001 aaagatttcc catctgtatg tgctccaacc ccagctattg tataatcgct tggtctccta
     3061 gccctctcgc ttgatattat tacccatctg tttctaatag ggtcaaacct gagctcacgc
     3121 attcaattct ccataataat cattaagccc tatgattata tcaagatttg atacataaac
     3181 tatattagag aaattaaggg aaaagcaaaa taaaatctca ggatcttgag ggaaaacaat
     3241 gggttgtaac aattcaaagt caattgcttc tttgtagatt gctactccgc tatattttaa
     3301 gttaatcaag cctgtaagtt gactaaactt tactcatttt ttactcaaaa tcaaccccct
     3361 attttactta tgattaaatt ttatataatt ctaaaatctt caagtgattt ttataaggaa
     3421 gaaaccaatg gtattctttt aaccttatct atattattat gctttaacgt ttaattttca
     3481 gaatataatc ctgccagaaa gactggcagg atacccctgt atttgggtag acaatgagtc
     3541 ttgctcgttg tcttaaatgc actaacctac aatgctgcat ttcaatatga aattggtgct
     3601 atatcttaga ttaatgatta cataaaacta atagtcaacg cttaaaatat caaacaaagg
     3661 agatttaagt ttaatgaatg cagaaagccc cattacagat cacgacatat ttctatttaa
     3721 gcaaggaagt catttcaaac tttacaacaa acttggctct catcccataa ctgttaacga
     3781 aacgcaagga acttactttg ctgtttgggc tccgaatgca gaaagggttt cagttatagg
     3841 agactttaat ggttggaatc ctgagtccca tcccttaggt gtgagatggg atggttcagg
     3901 tgtatgggaa ggatttattc cttatcttga aaaaggagca acttataaat atcagatcac
     3961 ctctaaacat aataactaca atgtagaaaa agcggaccct tacgcattct tctctgaaag
     4021 ccctcctaag acggcttcaa tcatatggga cttaaactat gattggaaag atgatgaatg
     4081 gatgaaaaat aggtttaaat ttaattcgct tgattctcca ttttccatat acgaggttca
     4141 tcttggctca tggagacgtg ttccagaaga aggaaacagg tttcttactt acagagaaat
     4201 agcccactgc cttgctgatt atgttaagta catgggattt acacatgttg aatttttacc
     4261 agtaatggag cacccatttt atggttcttg gggttatcag acatcaggat actttgcgcc
     4321 tacaagtaga tatggaaccc ctcaggattt catgtaccta gttgattacc tgcatcaaaa
     4381 cggaattggt gtaattttag attgggtccc ttcacatttc ccggacgata agcacggact
     4441 tatttatttt gatggcacac acctatatga gcatgcagac ccaaggaggg gttttcaccc
     4501 agaatggaat agttacattt ttaaccacgg ccgaaacgaa gtgagggctt ttctaatcag
     4561 taatgccctc ttctggctgg ataaatacca tattgacggc ctccgagtgg atgcggtggc
     4621 ttctatgcta taccttgac
//
LOCUS       DATDRL010000217        10520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3448599, whole genome shotgun sequence.
ACCESSION   DATDRL010000217 DATDRL010000000
VERSION     DATDRL010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10520
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3448599"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="VI935_07430"
     CDS             <1..1403
                     /locus_tag="VI935_07430"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HLE25467.1"
                     /translation="DNGREILFARGNVDVDLDFKKIGENLDQAYFDITMKSSGVDLSP
                     LVKLSEEIKNIKGLAVLDFRAYGKVSSPNIKGQVKLQDVLLRIHSLRDEIKVTNGIIE
                     MDGEKGFLKTLEIQTEEGKGTFEGDINFNELSYNINGKLDNLQIMPKGITASVDGNVE
                     IKGSDGKIHIGGNTKVRSARIKLPDLPTKKIEEIKFVDEEEEFTIKETEETDYYRDNV
                     AMDLNVNLPGNAWVKGKGANIEIKGKIGVTKKYTKPLILTGNITTVRGTYEFFGKLFK
                     IQRGEVSFRGTPEINPFLDVTALYKVSNVNVFVNVSGTAEKPKIELSSEPPMDQTEIF
                     SYLAFGTSSEKLGVGERVSLEQKATQAVALLAAGQLKGIVGEQFGLDIISITGGQGGF
                     KGTELEVGKYFTDKLYIAYQRSSPFNQTVIQSASTSPDVIDQVHVEYNFFDFLTLESE
                     ISGYQSGADVFYNFSY"
     gene            complement(1400..2587)
                     /locus_tag="VI935_07435"
     CDS             complement(1400..2587)
                     /locus_tag="VI935_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008683125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLE25468.1"
                     /translation="MQILAEEFSLIKRLPPYVFAIVNELKMKARGRGEDIIDLGMGNP
                     DQPTPKHIVDKLIEASQNPRNHRYSASRGIPKLRTAICDWYKRNYGVELNPETEAIVT
                     IGSKEGISHLMLSILAPGEVVVVPDPTYPIHSYSVIIARGDVKSVPLKKDINFIEAME
                     KAVKEIWPKPKVLMISFPHNPTTSVVDLHFFKEVVDFAREFGLIVVHDFAYADLCFDG
                     YKAPSFLQVPGAKEIGVEFFSLSKSYSMPGWRVGFMLGNPKIINALGRIKSYLDYGIF
                     QPIQIASISALNGPKEYVEQIREKYSSRRDRLIEGLGRAGWEIPKPMGTMFVWAEIPD
                     RLKKIGSLEFSKLLIENAKVAVSPGIGFGNYGEGYVRFALVENEQRIMQAVRGIRKVL
                     NSY"
     gene            3034..3678
                     /locus_tag="VI935_07440"
     CDS             3034..3678
                     /locus_tag="VI935_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HLE25469.1"
                     /translation="MKKGAWLLVLGLPLLFSCASITKKGTESYQLDGNTQIGIASWYG
                     NQEHGNKTASGERFSKYDYTAAHKTLPIGTMVRVTNLDNGQDVIVRINDRGPFKKGRI
                     IDLSYAPAKAIGMFGKGTAKVKVEVLSVPREASNYFEAKYTVQVASFADQQNAYNLKG
                     ILDDKHKDVRIENVDFGDYTFYRVKIGQFSDKSKAEKIAASLRRTGYIGKVIQE"
     gene            4069..4869
                     /gene="panB"
                     /locus_tag="VI935_07445"
     CDS             4069..4869
                     /gene="panB"
                     /locus_tag="VI935_07445"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HLE25470.1"
                     /translation="MKKVTVPELCDMKRRGEKIVMVTAYDSATANIIDEAGVDIVLVG
                     DSLGNVIQGLPNTLPVTMDEMIYHTKIVSKGIEHAHLCGDMPFMSYHASKEDAIRNAG
                     RFIKEGGAESIKLEINENYLDTIFSIQKAGIPVMGHIGLCPQSIHITGGYKIQGREIQ
                     EAKRLIELAKSTEEAGAFSIVLESIPKDLAKKITESINIPTVGIGAGPFCDGQVLVIN
                     DLLGLSPAPLPRFVKRYVNLREIMTKAVRDFVDEVREGVFPDVDHSYN"
     gene            4880..5719
                     /gene="panC"
                     /locus_tag="VI935_07450"
     CDS             4880..5719
                     /gene="panC"
                     /locus_tag="VI935_07450"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HLE25471.1"
                     /translation="MRIISSIREMQSLVNKVIKNGMSITLVPTMGALHEGHMNLINEA
                     RKRGDVLVVSIFVNPTQFGPNEDFTNYPRDLEGDLRKIENLGVDIIFAPTQEEIYPVE
                     FQTYVEVKELQEHLCGHFRPGHFRGVATVVLKLFNIVKPHVALFGVKDYQQLKIIQKM
                     VKDLNLEVEVIGYPTVRNEDGLALSSRNVHLSPSERSSASQVSKALREIKRKFDMGCR
                     NTKLMVEFGRKILEEEGINKIDYLEICDSETLENKELASRGDLLAVAVRIKGTRLIDN
                     IRL"
     gene            5733..6137
                     /gene="panD"
                     /locus_tag="VI935_07455"
     CDS             5733..6137
                     /gene="panD"
                     /locus_tag="VI935_07455"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HLE25472.1"
                     /translation="MNRMLLKSKIHRVTVTEANLDYEGSITIDKDLMDAADLVSYEQV
                     HIFNLTNGQRFFTYVIEGKPGSGVICTNGAAAHLAREGDTVIIASFASYNENEEKRHE
                     PKLVYVNGKNKIKKIKPEPNKLTLAYSVKPLI"
     gene            complement(6274..7335)
                     /gene="phaC"
                     /locus_tag="VI935_07460"
     CDS             complement(6274..7335)
                     /gene="phaC"
                     /locus_tag="VI935_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HLE25473.1"
                     /translation="MNKRMSESLREKAQKLLEALKLGLVEQPEVKVGTTPHEVVFTEN
                     KLRLLHYIPLAEKVFPTPLILIFALVNRPYILDLQPGRSVVEVLLKHGIDVYLIDWGI
                     PGDEDKHQDLDFYINKYIRKVVNKVKKISASDKVSILGYCMGGTMSVMYTALYPEDVK
                     NLIVMTTGIDFKASPSGLLNLWGEKSYFNVDEFVEAYGNVPAEFLQSIFIFMKPIENL
                     VTKYVTFYEKLDDKKIVENFLAMEKWLNDNIPVPGEVFREFIKYCYQENLLVQNKLKI
                     KGKVIDLRKIKCPVLNLIAEKDHLVPPGTSIVLEDLISSKDKETIVFPSGHIGLSVSS
                     KSLKELWPKVAGWLAKRSN"
     gene            complement(7602..7676)
                     /locus_tag="VI935_07465"
     tRNA            complement(7602..7676)
                     /locus_tag="VI935_07465"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7642..7644),aa:Thr,seq:cgt)
     gene            complement(7847..8320)
                     /locus_tag="VI935_07470"
     CDS             complement(7847..8320)
                     /locus_tag="VI935_07470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HLE25474.1"
                     /translation="MRKESKEQIDMFSDKSGNCKFCKIIRGEIQSYVVFEDNMSFAFL
                     DHRPLIPGHCLLVPKEHYRTLIDLPTALIAPLFINSKLLAQAAELGLEAEGSFVAINN
                     RVSQSAPHLHIHIVPRRRKDGLKGFFWPRQSYKDEDAKLKVQEAIRSAISKIQSM"
     gene            8584..9066
                     /locus_tag="VI935_07475"
     CDS             8584..9066
                     /locus_tag="VI935_07475"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HLE25475.1"
                     /translation="MGEKQIFTIGHSNRSLDEFVKVLKHYNIGVLADIRHFPRSKRNP
                     QFNREALEIKLPESGIRYIWIERLGGFRKGGFEEYMKTLDFQDGLGTLLEIAKEKAAA
                     IMCSELLWFRCHRQHIASTLTKKKWRVFHIYDEMKYEAHRVLREKTNQLSLTFDDKVR
                     "
     gene            9069..9389
                     /locus_tag="VI935_07480"
     CDS             9069..9389
                     /locus_tag="VI935_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HLE25476.1"
                     /translation="MVKDKEPYYYSKTVRQAIFDELLEGMRTVRELSQALRISEKEVI
                     THLTHVKQSALQKGYRFIMKPSDCLNCGFIFEKRDKPKKPGRCPRCKEEHLTNPSFGL
                     GKLL"
     gene            complement(9651..>10520)
                     /locus_tag="VI935_07485"
     CDS             complement(9651..>10520)
                     /locus_tag="VI935_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25477.1"
                     /translation="ERLPVCERHKGFSIKKSAPIMRCIDVFSLAGELNIRHKPISVFF
                     SSGAPQNLSTLSRMTVFINMYVYRFKLISKEIARKYLEDYSIIKDHDDSTIARLLIIW
                     LRGHDLGHFYGLDSLGKKMSELDKAYLILHELKSDLTALYNMRYLADDLLKDDLLIKA
                     YIFAIAEMFRYIRRGRFYNYPDTASAFLAYCYFRESGSIRFDTKGKKFTVDFGKLETD
                     LKKLTEKLLTIFAEGDVTEGLKLLNRWGDIKELGQHGFPNELEFLEDPDIPHYIDFNF
                     VTRDGILSGLERR"
BASE COUNT         3392 a         2021 c         2093 g         3014 t
ORIGIN      
        1 aggataatgg gagagaaatt ctatttgcaa gaggtaatgt tgatgtagat ctagatttca
       61 aaaaaatcgg agaaaatcta gatcaagcat atttcgatat tacaatgaaa tcaagcggtg
      121 tggacttgag cccactcgta aagttgagtg aagaaattaa aaacattaaa gggctagccg
      181 ttcttgattt cagggcatat ggaaaggtga gtagccctaa cataaagggg caagtgaaac
      241 ttcaagatgt tctattaaga atccattcac taagagatga aattaaagtt actaacggaa
      301 ttattgagat ggatggagaa aagggatttc ttaaaacact agagattcaa actgaggaag
      361 gtaaaggaac atttgaagga gatataaatt tcaatgaatt atcttataac ataaacggca
      421 aattggacaa tttgcagata atgcccaagg gaattaccgc gagcgtggat ggaaatgtag
      481 agattaaagg aagtgatgga aaaatccata taggtggaaa cactaaagtc agatcagctc
      541 ggattaaact cccagatcta cctacaaaga aaattgagga aataaaattt gttgacgaag
      601 aagaggagtt tacaatcaaa gaaactgaag aaacagatta ctacagagac aatgtagcaa
      661 tggatttaaa tgtcaatctt cctggaaatg catgggttaa gggtaaagga gcaaacattg
      721 aaataaaagg aaagattgga gtcacaaaaa aatataccaa accattaatc ttaacaggca
      781 acatcactac agtcagagga acatacgagt ttttcggaaa actctttaaa atccaacggg
      841 gtgaggtgag ctttcgagga acccctgaga ttaatccatt tctagatgtg acggctttat
      901 ataaggtatc aaatgtgaat gtatttgtaa atgtaagtgg cactgctgaa aaacctaaaa
      961 tagaactttc tagtgagcct ccgatggacc aaacagagat cttctcctac ctagcatttg
     1021 gaacatcctc agagaaactc ggcgttggtg aaagggtttc cctagagcaa aaagccacac
     1081 aagcagtagc acttttggcg gcaggccagc tcaagggtat agtcggagaa caattcggac
     1141 tagacataat cagtataacg ggtggacaag gcggttttaa aggtacggaa ttagaagtag
     1201 gcaaatactt taccgataaa ctatacatag cataccagcg tagttcccct tttaatcaaa
     1261 cagttataca atccgcctca acttcaccag atgtaataga tcaagttcat gttgagtata
     1321 atttctttga tttcctaacc ttagaaagtg agataagtgg ataccaaagc ggagccgatg
     1381 tattttataa ctttagttat taataggaat tcaaaacctt tctaatacct cttactgctt
     1441 gcatgattct ttgttcgttt tcaacaagcg caaacctaac atatccctcg ccataatttc
     1501 caaatcctat tccgggcgaa acagcaacct tcgcgttttc gataagtaat tttgagaatt
     1561 cgagggagcc aatttttttt aatctatctg ggatttctgc ccaaacaaac atcgttccca
     1621 taggctttgg aatctcccaa cctgctcgtc ctagcccttc aataagtctg tcccttctgg
     1681 aactgtattt ctctcttatt tgttcaacgt attctttagg accgtttaac gcggaaattg
     1741 atgctatctg aataggctga aaaatcccgt agtctagata gcttttaatc ctaccaagcg
     1801 catttattat ttttggattt cccagcataa acccaacccg ccatccaggc atgctgtagc
     1861 tctttgaaag tgaaaagaat tcaaccccga tttccttagc acccggcacc tgtagaaaac
     1921 tcggcgcttt atatccgtca aagcatagat cagcataagc aaaatcatgc acaacgatta
     1981 gtccgaattc cctagcaaag tcaactacct ccttgaagaa atgtaagtca acaacgcttg
     2041 ttgtgggatt atgtggaaag gaaatcatta aaacctttgg cttaggccat atctccttga
     2101 ctgccttttc cattgcctca ataaaattta tgtccttctt aaggggcacg cttttcacat
     2161 ccccccttgc gataataacc gagtaactgt gaatcggata agttggatca ggaaccacaa
     2221 ctacctcgcc tggagcaagt atcgagagca taagatgaga tatgccctcc ttcgaaccaa
     2281 tcgtgacaat agcttcagtt tcaggattta attcgacacc atagttcctt ttataccaat
     2341 cacaaatagc agttcgtagc tttggaatac cacgagatgc agagtatctg tggtttctag
     2401 ggttttgaga ggcttctatc aatttatcga caatatgctt tggtgttggt tggtctgggt
     2461 ttcccattcc aaggtctata atatcctctc ccctccctct tgccttcatc tttaattcat
     2521 ttactatagc aaacacatat ggaggaagtc tctttataag cgaaaactct tctgcaagaa
     2581 tttgcatctt tattgtctcc caatactaat tgagcgattt tagtaatttt attctaatct
     2641 tcgataatct caagaagtaa ccttagtccc aattcctaat aaattacgaa tgcctccaac
     2701 gggtatccaa cgagtcttgc tcattggctg tataaccacc tcctctggta gcttacgatc
     2761 cttgcccgta acctgattac ccgctcgtaa cacttatggt ctacactccg tcatttcgag
     2821 cacattcgcg gagtctgtac tcagagaatc gaatgtactc agtgtaagct cagctgagaa
     2881 atctagatca ctcgcgtgcg ctcgagatga caaattccta actacgacaa ggttgataag
     2941 gacagggttc gagattgaca aaatacgttt ctctaccatc cactaaaaag gttctttata
     3001 acctaaacta gatatgttaa aattcttggc aatatgaaaa aaggggcatg gttacttgta
     3061 ttgggtcttc cattgctttt ttcatgcgca tcaattacta aaaaaggtac agaaagctac
     3121 caacttgacg gcaatactca aataggcatt gcttcttggt atggaaatca ggaacatggt
     3181 aacaaaactg caagcggtga acgcttcagt aaatatgatt acacagccgc ccataaaacc
     3241 ctcccaatag ggacaatggt cagggttacc aatttggaca atggacaaga tgttattgtt
     3301 agaattaatg acaggggtcc gtttaaaaaa ggacgtataa ttgacctttc atatgctcca
     3361 gccaaggcta taggaatgtt cgggaaggga acggcaaagg ttaaggttga ggtgctttcg
     3421 gtcccaagag aagcatcaaa ctactttgaa gctaaataca cggtccaagt tgcttcattt
     3481 gcagaccagc aaaacgctta caatcttaaa gggatcttag acgataagca taaggacgtt
     3541 agaattgaaa acgttgattt cggggattat accttctata gggtaaagat agggcagttc
     3601 tcagataaaa gtaaagccga aaaaatagcc gcaagtctta gaagaacagg atatatagga
     3661 aaggtaatac aggaataaat tagtggttcg tggctcgggt atcgtggatc tgggcacgag
     3721 tcgcaaatca cgaaccacga ttcacgaatc agctaaattt gacttcccga tcaagtttct
     3781 ttattctaac tttaaatctc tcgcaattgc gtagcaagct tataggctat tgttcattat
     3841 ttataccgct tggatcctct agaggaccga gacggaggaa acttggataa gaagataaat
     3901 aggacggaac atgagccgta cctaaatagg ttataaaact aaaaccagcg agggtttaag
     3961 cccgataagc tggtttttta tttgtaagta atagccacga aggcacaaag aaaacaaaat
     4021 ccttttatct tcgcgtcttg gtgtcttagt ggctacaaaa gaacaaaaat gaaaaaggtc
     4081 acagttcccg agctttgtga tatgaagagg cgtggagaga aaatcgttat ggttacagcc
     4141 tatgattctg ccacggcgaa tataattgac gaagccggag tagatattgt ccttgtcggt
     4201 gattccttag gaaacgtaat tcaagggctt ccgaacaccc ttcccgtaac aatggacgaa
     4261 atgatttacc acacaaaaat cgtctcaaaa ggaatagagc atgcccacct ctgcggagac
     4321 atgcctttta tgtcatacca cgcttcaaag gaagatgcca taaggaatgc tggaagattc
     4381 atcaaggaag ggggagcgga atctataaaa cttgagataa acgagaatta tcttgataca
     4441 atcttctcta ttcaaaaggc cggtatccct gtaatggggc atattgggct ctgccctcag
     4501 tcaatacaca taactggggg ttataagatt cagggacgcg aaattcagga agccaaaagg
     4561 cttatagaac ttgcaaagtc taccgaagag gccggagcat tttcaattgt tctagagagc
     4621 atcccaaaag accttgctaa aaagataact gaatctatca atattccaac tgtcggaatt
     4681 ggagcaggtc cattctgtga cggacaggtg cttgttataa atgatctatt aggcctttct
     4741 ccagcaccgc ttcctagatt cgtaaaaaga tatgtaaatt taagagagat tatgacaaag
     4801 gccgtaaggg attttgttga tgaagtaaga gagggtgtat tcccagacgt tgaccattca
     4861 tataactgag gaaaaccacg tgaggataat aagcagcata agagagatgc aaagcttagt
     4921 aaacaaggtg ataaaaaatg gaatgagtat cactcttgtt cctactatgg gggcgcttca
     4981 cgaagggcat atgaatctta tcaatgaggc taggaaaaga ggagatgtgc ttgtagtaag
     5041 catttttgta aaccccacac agtttgggcc caatgaggac ttcacaaact acccaaggga
     5101 tttggaaggt gatttaagga agattgaaaa cctcggtgtg gatattattt ttgccccaac
     5161 ccaagaagaa atttatcccg tagaattcca aacatacgtt gaagtaaaag agcttcaaga
     5221 acacctttgt ggacactttc gtccaggaca ctttaggggt gtggcaaccg tagtccttaa
     5281 gttattcaat atcgtgaaac ctcacgttgc gctttttgga gtgaaggatt atcagcaatt
     5341 aaaaatcatt caaaaaatgg taaaggattt aaacctagag gtagaagtta taggatatcc
     5401 cactgtcaga aacgaagacg gactggcact tagttcaaga aacgtacatc tctcaccgag
     5461 tgaaagaagt agtgcatctc aagtttctaa agcacttcgt gaaataaaga gaaagtttga
     5521 catgggttgt agaaacacta aactcatggt tgaatttggt aggaaaatat tagaggagga
     5581 aggaatcaac aaaattgatt acctagaaat ctgcgattcc gagacccttg agaacaaaga
     5641 actagccagt agaggagatt tactagcagt agctgtgagg atcaagggaa caaggcttat
     5701 tgacaacata agactttaag gaggagatgg acatgaatag gatgcttcta aaatcaaaaa
     5761 ttcatagggt gaccgttaca gaagctaatc ttgactatga aggaagcatc acaattgata
     5821 aagaccttat ggatgctgct gatttggtct cttacgaaca agtccatatt tttaacttga
     5881 caaatgggca gaggtttttt acatatgtta tagaaggcaa gcctggctca ggtgtcattt
     5941 gcacaaatgg agccgcagcc caccttgcaa gagagggaga cacagtaatc atagccagct
     6001 ttgcttcgta taacgaaaac gaggaaaaaa ggcatgaacc taaattggtt tatgtaaacg
     6061 gaaagaataa gattaaaaaa ataaagccag aacctaataa gttaacgctt gcatatagcg
     6121 tcaaaccgct tatttaaatg tttttttcat ttttctattt tccacaatat ttcgatttct
     6181 tactataaaa gttgggattt gttcatcgtc ctaccctaaa ttttagaact cggaagctta
     6241 aaagatagag cattttatag ccggcatacc ttcctaattc gatcttttag caagccaccc
     6301 tgcgactttt ggccagagtt ctttcaaaga cttgctactt actgaaagcc caatgtgtcc
     6361 acttggaaag actattgttt ctttatcttt gctagatatt agatcctcta aaactatact
     6421 ggtaccaggg ggtacaagat gatccttctc agctataaga tttaacacag gacattttat
     6481 ttttcttaga tcaattacct ttcctttgat tttaagtttg ttttgtacaa gcaggttttc
     6541 ttgataacaa tactttatga actctctaaa aacctctccc ggaactggga tattatcgtt
     6601 tagccacttt tccatagcga ggaaattctc aacgattttt ttgtcgtcta atttttcgta
     6661 aaaagtgaca tattttgtga caaggttctc aattggtttc ataaaaataa aaatggactg
     6721 gagaaattca gcagggacat ttccataggc ctccacaaat tcgtcaacat taaagtacga
     6781 tttttctccc cataaattta acaacccact tggcgaagct ttaaaatcaa tacccgtagt
     6841 cataacaatt aggtttttta catcttcagg ataaagtgct gtgtacatta ctgacattgt
     6901 tccacccata cagtagccaa gtatacttac tttatctgag gctgagattt tctttacttt
     6961 attcactacc tttcgtatgt atttgtttat ataaaaatct agatcctgat gtttatcttc
     7021 gtcaccgggg attccccaat ctatcagata tacgtctatc ccatgtttta agagtacttc
     7081 aacaacactt cttccaggtt gcaaatccag tatgtatggg cggttcacaa gtgcaaaaat
     7141 caaaataagg ggagtgggaa ataccttttc agctagaggt atataatgta gaagcctaag
     7201 tttattctct gtaaagacaa cctcgtgagg ggttgtgcct accttaacct cgggttgttc
     7261 tactaagcct agtttaagtg cttctagaag cttctgggcc ttttctctta aggactcact
     7321 cattctttta ttcattattt ttatgaattt ctattgcaca gcaccttatg gattgctaaa
     7381 tattaccgag tcttagtgga atattctagg aattcttgag ggacgattat ttgggcaact
     7441 tgacgaaact aggacttgga aaaatttatt taatcggcca caaggatttt taacagataa
     7501 tccagaaaat gacttaaagt cctttagtaa gtttgctagc gaagaagcat caaaaataaa
     7561 agaattagtc catcctagtt gtcttaaaac aaaaatatta ttggagccga cgaagggatt
     7621 cgaacccttg acctgcgcat tacgagtgcg ctgctctacc actgagctac gtcggctatt
     7681 ctttataatt atctgattaa taataaatat ataccgtcaa aattcaccac ttgatacaaa
     7741 cctgcgataa ctcctttact ctaaaaacac gcgataaaag tttagctgag ttttagaaca
     7801 atcgtcaagg ttagataaca tcttacaact atatatcgta ctagtctcac atagattgaa
     7861 tcttggaaat tgcagaccga atagcttcct gcacttttag ttttgcatct tcatccttgt
     7921 aagattgtct tggccaaaag aaacctttca agccatcctt ccttcgtctg ggcacaatat
     7981 ggatgtgtaa atgtggcgcg ctttgactga cacggttgtt gatggctaca aacgatccct
     8041 cagcctctag gcctagttct gctgcttgtg ctaaaagctt tgagttaata aaaagtggag
     8101 cgataagagc cgttggcaga tcaatcagag ttctgtagtg ttcttttgga acaagtaggc
     8161 agtgtccagg aattagtggc cgatgatcca aaaaagcaaa agacatattg tcttcaaaca
     8221 ccacgtagct ttgaatttct ccccgtatga tcttacaaaa tttgcaattc ccacttttat
     8281 cactaaacat atctatttgt tccttacttt cctttctcat ggtagttaaa tcttactgga
     8341 acttttgccc ttccctcctc ctaggctagt ctaaaagtac atatctaggc gtggtgcttt
     8401 atgtcactag caagattata tcctaaataa ccttatttga tgccaatgga atttaagtct
     8461 ctcgttagat acaactttgc gaaattgata aagcttattt tcttaagcga aatctcgctt
     8521 tagactttgc taagttgcta tatggtaaat agatagctga tggtggagca tcaaaataac
     8581 gatatgggtg aaaagcaaat ctttacaatc gggcactcaa atcgaagtct cgatgagttt
     8641 gtgaaggttc taaaacacta caatatagga gttctcgctg atattcggca tttcccccgt
     8701 tcaaaacgca acccccaatt caacagagag gctcttgaaa ttaaactccc agaaagcggc
     8761 attcggtata tctggatcga aaggcttgga ggattcagga agggcggatt tgaagaatat
     8821 atgaaaacat tggatttcca agacggacta ggaacacttc ttgagattgc aaaagaaaaa
     8881 gcagcagcca tcatgtgttc cgagctcctt tggtttcggt gccaccgtca gcatattgct
     8941 tctaccttaa caaaaaagaa atggcgggtt tttcatattt atgatgaaat gaaatatgag
     9001 gctcatagag tccttagaga gaaaacaaat cagctaagtc taacttttga tgataaagta
     9061 aggtaaaaat ggtgaaagat aaagaaccgt attattattc taaaactgtt agacaagcca
     9121 tatttgatga actcctagag ggtatgagga ctgtaagaga gttatcacaa gcactcagga
     9181 tttcagagaa agaggtgata actcatctca ctcatgttaa gcaatccgct ctacagaagg
     9241 gttataggtt cattatgaaa ccttcggact gcctgaattg cgggtttatc ttcgagaaaa
     9301 gagataagcc aaagaaaccc gggagatgcc ccaggtgtaa agaggaacat ctcacgaatc
     9361 cgagctttgg actggggaaa ttactctgac aatgaaatgc cctgcagcaa catgcaggat
     9421 atatgcgtat aaacagacgc taccttcttt catcgcgagg agcaattgct ggattagaca
     9481 atcaagtcta tccacatata ccgatagtcg gggttttcca accccgcaac atgtgtgggg
     9541 caggaatttc ccgtctattc attttcttat ttgagggcca gtgagaaaaa aagtagtgtc
     9601 atccatttac ttagtgaaat agcccgcata gagatccaat tacacgcaag ttatctacgt
     9661 tctaggcctg agagaatccc atccctagta acaaaattaa aatcaatata gtgaggtata
     9721 tcgggatcct caagaaattc aagttcatta gggaaaccgt gctgtccgag ttccttaatg
     9781 tccccccagc gatttaagag tttcaatcct tcagttacat caccttcagc aaatatcgta
     9841 agcagttttt cagtcagttt tttaagatca gtttctaatt tgccaaaatc tactgtgaat
     9901 ttttttcctt tggtatcaaa cctaattgat ccactttctc taaaatagca gtatgcgaga
     9961 aatgcagaag cggtatcggg gtaattgtag aatctacccc gtcttatata tctaaacatc
    10021 tctgcaattg caaaaatata tgctttaatg agtagatcat ctttaagtag atcatcagca
    10081 aggtatctca tattgtaaag tgctgtcaaa tcagatttaa gctcatgtag tatcaaatat
    10141 gccttatcca gctcagacat cttttttcca agagaatcta gcccgtaaaa atgtccaagg
    10201 tcatgacctc gaagccatat gattaaaagc ctagcaatgg tgctatcgtc atgatctttt
    10261 ataattgagt aatcctcaag gtattttctt gcaatctcct ttgagatgag tttaaaccta
    10321 taaacataca tattgataaa cacagtcatt cttgaaaggg tagaaagatt ttgaggggcg
    10381 ccacttgaaa aaaaaacaga gataggttta tgtcgaatat ttagttctcc cgcaagtgaa
    10441 aatacatcaa tacatctcat aataggtgca cttttcttaa tggaaaaacc tttatgccgt
    10501 tcgcagaccg ggagtctttc
//
LOCUS       DATDRL010000218         8241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1145271, whole genome shotgun sequence.
ACCESSION   DATDRL010000218 DATDRL010000000
VERSION     DATDRL010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8241
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145271"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VI935_07490"
     CDS             <1..868
                     /locus_tag="VI935_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HLE25478.1"
                     /translation="AMAIRAIPFEILNFSELNLPPILEKISLEERGLIIATGTTSSGK
                     STTLAAIIDCINSTRARHIITIEDPLEYVHRDKKSIIQQREVDIDTLSFTNALRSAMR
                     QDPNVILVGEMRDLETMEIAMAAAETGHLVLTTLHTLDAQETINRILTIFPPNQHNQI
                     RYQLSQILKAIISQRLMPIADGKGRVPAVEVLIATARIRECISDPERTAEIRTAIEEG
                     YLHYGMQTFDQCLLDFYKKGLITYEEVMRQATNKDDLALKISGITSGADAMAEEIDRR
                     GKEKAKHESLRN"
     gene            849..1532
                     /gene="tsaB"
                     /locus_tag="VI935_07495"
     CDS             849..1532
                     /gene="tsaB"
                     /locus_tag="VI935_07495"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HLE25479.1"
                     /translation="MKVLGIETSTGSGSVAVLECDKVLGEFFLNLGPTHSEKLLPMID
                     WLLKEVGVEKNEIEGIAVSKGPGSFTALRVGISTAKGLAFTLGIPVVGVSSLEVLAWN
                     LLFTPLYICSLIDAKKKEVYAALFRYSDTNLNRITDDILISPDDICEMISERTIFVGN
                     GALLYRDFLINSLGELALFCPSGVNFPKASSCALIGSYKFKNGNKDDLSALAPQYLRK
                     SEAEIYREG"
     gene            1537..1758
                     /locus_tag="VI935_07500"
     CDS             1537..1758
                     /locus_tag="VI935_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HLE25480.1"
                     /translation="MKEMEIIERLLEGDEEFKRLYFEHRKLDDTVKELEKKGTLSIDE
                     ELEVKRLKKTKLALKDKMEEKIRRIRKSF"
     gene            1777..3480
                     /gene="ilvB"
                     /locus_tag="VI935_07505"
     CDS             1777..3480
                     /gene="ilvB"
                     /locus_tag="VI935_07505"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HLE25481.1"
                     /translation="MVRMIGAQMFFETLLDQGVDIIFGLPGGYVLKVYDVMPKYTNVI
                     KHVLSRHEQGATHMADGYARASGRPGVVLVTSGPAATNTVTGITTAHMDSSPVVVFTG
                     QVPTPYLGSDAFQEADHIGITRPCTKHNSLVRKTKDLPQAMKEAFYIAVTGRPGPVLV
                     DMPKDVLIGEDEFEIPKEISLRGYKPTTKGHIGQIKKAVEMILSAKRPVIFGGGGLIW
                     SEATPELIEFVHRLKIPVALTLMGLGSYPASDPYYMSMLGMHGSYKANMAVHESDLII
                     AIGARFDDRATGGNFPKFAPNAKIVHIDIDPSTIDKNIRVHCPIVGDAKQILKQMLEL
                     IPSNLQIDRREWFGMIEEWGREHPLTYRQNGDKIMTSFLIDTLCKITNGDAVIVTDVG
                     QHQMWVAQFYKFNRPRTHITSGGLGTMGFGFPAAIGAKFARPDEMVICVSGDGSFQMN
                     MQELATAVENNMDLKIIVINNEHHGMVRQWQTLFYNSNYSASHFDVMPDFVKLAEAFG
                     AKGLRAKRPDELEATLREGLFTEGVVLIEVVVDYEEMVYPMIAPGGAMNEMILEPADI
                     T"
     gene            3684..4187
                     /gene="ilvN"
                     /locus_tag="VI935_07510"
     CDS             3684..4187
                     /gene="ilvN"
                     /locus_tag="VI935_07510"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HLE25482.1"
                     /translation="MRHTISILVDNEPGVLARIAGLFSARGFNIESLCVAETLDQTSS
                     RITLVTSGDDWIIEQIIKRFNNMINVIKVQDLIEVTHVEREMVLVRVEATQETRAEIL
                     RMADIFRAKVIDVGPKSYALELTGDKEKVNAFIELLKPMGIKEIARTGVVAMAREVQN
                     HKRGGER"
     gene            4184..5200
                     /gene="ilvC"
                     /locus_tag="VI935_07515"
     CDS             4184..5200
                     /gene="ilvC"
                     /locus_tag="VI935_07515"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HLE25483.1"
                     /translation="MKVYYDKDVNAEKLKTRKIAIIGYGSQGHAHAQNLRDSGMNVVV
                     GLREGGSSWEKAKNAGFKVLPVDEASRWADIVMILAPDSHHSDIYSNHISNNLNPGDS
                     VAFGHGFNIHFGQIVPPPNVDVFMVAPKAPGHTVRNEYTQGRGVPVLIAIYQNATGQA
                     KEIALAYAGAIGAGRAGIIETTFKDETETDLFGEQCVLCGGLTALIIAGYETLVEAGY
                     PPEMAYFECLHEVKLIVDLIYEGGISNMRYSISDTAKYGDITRGPRVINDTVRQEMKR
                     ILKEIQSGEFAREWIIENRAGRPVYNALLKKGESHQIEEVGRRLREMMSSLFKQKLVD
                     KTKN"
     gene            5270..5926
                     /locus_tag="VI935_07520"
     CDS             5270..5926
                     /locus_tag="VI935_07520"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HLE25484.1"
                     /translation="MIKPRSIIAVEGLPYIGISAFLAWITALFGFESLSIILAAVTVF
                     IIYFFRDPERKTPEVPLSIVSPADGKVVEIDNALEENFLNLEMKRIGIFLSITDCHVN
                     RFPLTGKVLNTKYTPGQFHIADLKRSSKENERLATLIESDDKEQIVVVQIAGFIARRI
                     VSYVSVGDLLQKGERFGMIKFGSRVDIYLPPSSEVSITLGDKVRGGETIIGFLRGKGE
                     "
     gene            5923..6768
                     /locus_tag="VI935_07525"
     CDS             5923..6768
                     /locus_tag="VI935_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HLE25485.1"
                     /translation="MKIRTSSVFTNDSKRKRSKRQRMLPVLPNLFTTGSLFCGLLSIM
                     TSIQIASLNEIQASDSEWFYRKFWWAAAFIGIAGLLDLFDGRIARILKSQSNFGLTYD
                     SLSDLVSFGVAPGVLIYVFTLMQSGKLGLMVALFYIVCTALRLARFNVQSGNREKYAF
                     TGLPSPVAAGLMFSPILLFSEFQITSDDRMMWFYLILAPFVGLLMVSDVPYWKGLRLG
                     WAKPFNVLVVAAIIFTAVITNPEIMVLLVVYLYSLIGLLLYIISQFKEKPKVIEETEP
                     EEETP"
     gene            6836..>8241
                     /locus_tag="VI935_07530"
     CDS             6836..>8241
                     /locus_tag="VI935_07530"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HLE25486.1"
                     /translation="MRYFFNYSECNIRGMVIKVLASGILILTLMVGDKIMAAEREKAA
                     KYIEKGLDGGIGVVKYQLENGLTVILEENHSASVVAVNVWVEVGSACEQDGEYGLAHV
                     HEHMVFKGTEKRGVGEIARVIEGDGGDINAYTSFDETVYYVVIASRFLDTALDVLSDA
                     MENSKFDPDELNKELEVVLEEIRRGEDTPSRKLSERLFSEAYTVHPYGRPVIGSDESV
                     RNFTRERVVNFYKKWYSPNNMVLVLVGDFDARKVIPKIQETFGALQERSIPGCVISDE
                     PAQKELRAFVFDKNIQEGYFSLAFHTPSVKHEDAPVLDVISSILGGGESSRLYRRVKE
                     EKGLVNNIYAYSFTPKSIGLFNVGGTLDPEKARDAFSEIIKETYRLKYEPVSFEELSR
                     AKVNIESDSIYTKETMQGQAQKLGYYEVEAGDYRLESDYLQRVSQVTQEDIIRVARRY
                     FNNPNLTAGFLLPTGKVT"
BASE COUNT         2449 a         1456 c         2032 g         2304 t
ORIGIN      
        1 agctatggct ataagggcga ttcctttcga gatcctgaat ttttctgagt taaatctacc
       61 gcctatactt gagaagattt ctctggagga gcgcgggtta ataattgcaa ctggaacgac
      121 gagtagcggt aaatctacaa cccttgccgc aatcattgat tgtataaact cgactcgagc
      181 taggcatata atcacgattg aagatcccct tgaatacgta caccgtgaca aaaagagcat
      241 tatacaacag cgagaagttg atattgatac actttcattt acaaacgctt taaggtccgc
      301 aatgaggcaa gatccaaatg tgattcttgt cggtgaaatg cgcgatcttg aaacaatgga
      361 aatcgctatg gctgctgctg aaactgggca tctggttctt acaacactcc atactcttga
      421 tgcgcaagag acgattaacc gaatactcac tatattcccg cctaatcagc ataatcaaat
      481 tcgctaccaa ctttctcaga tcttaaaggc gataatttcc cagagactaa tgcctatagc
      541 agatggaaag ggtagggttc ccgcagttga ggtgctgatt gctacagcaa ggattagaga
      601 atgtatctct gatccagaaa ggacagcgga aatccgaact gctattgaag agggatatct
      661 gcattatggg atgcagacct tcgatcagtg cttgttagat ttttataaaa aaggcctaat
      721 cacttatgaa gaggtcatga gacaggcaac gaataaagat gaccttgcgc tcaagataag
      781 tggtattact tcgggagcgg atgcgatggc agaggaaatt gatcgtcgtg gaaaagaaaa
      841 ggcaaagcat gaaagtctta ggaattgaaa catcaacagg ttctggaagc gttgcggtct
      901 tagaatgtga caaggtgctt ggggagtttt ttctaaatct cggtccgact cactctgaaa
      961 agcttcttcc gatgatagat tggctattaa aagaggttgg tgtagaaaaa aatgagattg
     1021 aagggatcgc agtttcaaag ggaccgggct cttttacagc actaagggta gggatctcca
     1081 cagcaaaggg tcttgctttc acccttggaa tcccagtcgt tggggtttcg tctcttgagg
     1141 ttcttgcatg gaatctgctt tttactcctt tatatatatg ttctctgatt gatgctaaaa
     1201 agaaggaggt atatgcagcc ttatttagat attctgatac caacttaaat aggattacag
     1261 atgatattct tatttctcct gatgatattt gtgagatgat ctctgaaagg acaatttttg
     1321 tcggaaatgg tgcattgctt tatagggatt tcttgataaa ctcgcttgga gagcttgcgc
     1381 ttttttgtcc ttcgggtgtc aactttccca aagcttctag ttgtgctctt ataggatcct
     1441 ataagttcaa aaacggcaac aaggatgact tatcggctct cgctcctcaa tatttaagga
     1501 agtcagaggc tgaaatctat agagaggggt gatataatga aggaaatgga gataattgaa
     1561 aggttactcg aaggcgatga agagtttaaa aggttatatt ttgaacatag aaagttggat
     1621 gacacggtaa aagagctaga aaagaagggt actctttcta tcgatgaaga gctagaggtt
     1681 aagagactaa agaagacgaa gcttgcactc aaagataaga tggaagaaaa gataagaagg
     1741 ataagaaaaa gtttttaaga tgggtggtag actccaatgg taagaatgat tggcgcacaa
     1801 atgttttttg aaacactact tgaccaaggg gtggatataa tctttggtct tcccggagga
     1861 tatgtgctaa aggtgtatga tgttatgcct aagtatacaa atgttataaa gcatgtcctt
     1921 tcaagacacg aacagggagc cacacatatg gcagatggtt atgcgagagc atctggaaga
     1981 cctggcgttg tgcttgttac atctggtcca gctgctacca acacggttac aggaattacc
     2041 accgcgcata tggattcttc tcctgtggtt gtctttactg gtcaggttcc aacgccgtat
     2101 cttggaagcg atgcctttca ggaggcagat catattggga ttactagacc atgcacgaag
     2161 cataatagcc ttgtcaggaa gactaaagat ctcccacaag ctatgaagga ggccttttat
     2221 atcgctgtta cgggacgtcc aggccctgta cttgttgaca tgccaaaaga tgttcttatc
     2281 ggggaggatg aatttgagat tccaaaagag ataagtctaa gaggctacaa accaacgaca
     2341 aaaggtcata taggacagat aaagaaggcg gttgagatga ttctctcagc aaagagacca
     2401 gttatctttg gtggtggagg tcttatctgg tctgaggcta cgccagaact catagagttt
     2461 gtgcataggc ttaagattcc agtagcgctt accttaatgg gacttgggtc ctatcctgct
     2521 agcgacccat actatatgag catgctagga atgcacggct cctataaagc aaatatggct
     2581 gttcacgaaa gtgatctaat catcgcaatt ggagcgaggt ttgatgatag ggcaaccggt
     2641 ggaaactttc ctaagttcgc accaaatgca aagatagttc atatagatat tgacccctcg
     2701 acaattgata agaacattcg tgttcactgt ccaatcgttg gagatgctaa acaaattctg
     2761 aaacagatgc ttgaacttat tccctcgaat ctacagattg acaggaggga atggtttgga
     2821 atgattgagg agtggggtcg agaacaccct cttacttaca gacagaatgg ggataagata
     2881 atgacctcat tccttataga cacgctctgt aagatcacaa atggagatgc tgtaattgtg
     2941 actgatgttg gccaacatca aatgtgggtt gcccaattct ataagtttaa ccgccccagg
     3001 acgcatatta catcaggcgg actcggcact atggggtttg gctttccggc tgcgattggt
     3061 gcgaaattcg cacgcccgga cgaaatggta atatgtgtgt caggtgatgg aagcttccag
     3121 atgaatatgc aggaacttgc cacagcggta gagaataata tggatttaaa gatcatcgtc
     3181 ataaataacg agcatcatgg aatggtaagg caatggcaga cgctatttta caatagcaac
     3241 tattcagctt ctcatttcga tgtaatgcct gatttcgtga agcttgccga agcattcggt
     3301 gctaaggggt taagagcaaa gagacccgat gagctcgagg ctacattaag agagggcctc
     3361 tttactgaag gggttgttct aatagaggta gttgttgact acgaagaaat ggtttatccc
     3421 atgatcgctc ctggtggtgc gatgaacgaa atgattctgg agcctgcaga tataacttga
     3481 taagtcaatt gagcttattt gattaggcat tcacccttga tcagtctcgg agaaaaattt
     3541 tattgcaaga aaagtgaaag cccaaaacgc ataaatttga cttgccattt aatacttagc
     3601 attagaggat ggggatttaa caccttattc acttcaatat ctgagataag tttcacataa
     3661 atttctaaac cggaggttta aaagtgaggc acacaatctc aattttggtt gataacgaac
     3721 cgggtgtact tgcaaggata gcggggcttt ttagtgcaag ggggtttaac attgaaagtc
     3781 tatgtgtggc agagacactt gatcaaacgt catcccgtat cactctagtt acaagcggcg
     3841 atgactggat aatagagcag atcataaaga gattcaacaa catgataaac gtgatcaagg
     3901 ttcaagatct aattgaagta actcatgtag aaagggaaat ggtgcttgta agagtagagg
     3961 ccactcagga aaccagggct gagattctca gaatggctga tatctttagg gcaaaggtaa
     4021 tagatgttgg tcctaaatca tatgcactag agcttactgg tgataaagag aaggtaaatg
     4081 cattcataga actattaaaa ccgatgggga taaaggagat tgcgcgcact ggcgttgtag
     4141 caatggcaag ggaggttcaa aaccataaaa gaggcggtga aagatgaagg tctattacga
     4201 taaggatgtt aatgctgaaa agctaaagac aagaaaaatc gcaataatag gctacgggag
     4261 tcaaggacat gcacatgcgc aaaatcttag agatagcggt atgaacgtag tggttgggct
     4321 tagggaagga gggagtagtt gggaaaaggc taagaatgct ggctttaaag tactccctgt
     4381 agacgaggcc tctagatggg cagatattgt tatgattctt gctccagatt cacatcactc
     4441 tgatatttat tcaaaccata tttcaaataa ccttaacccc ggggattcgg ttgcatttgg
     4501 tcatgggttt aacattcact ttggacagat tgttccacca ccaaatgttg atgtattcat
     4561 ggtagcgccg aaggcccctg gccacaccgt tcgtaatgaa tacacgcaag gacgcggtgt
     4621 tccagtgctt atcgcaattt atcagaatgc aacggggcag gcgaaggaaa ttgcccttgc
     4681 ttatgccggt gcgatcgggg ctggacgcgc tgggataatt gaaaccacct ttaaagacga
     4741 aactgagacg gatctttttg gtgagcagtg tgttctttgc ggcgggttga cagccttaat
     4801 tatagccgga tatgaaaccc tcgtcgaagc cggctatcca cctgaaatgg cttactttga
     4861 atgccttcac gaggtcaagc ttatagtgga cttaatttat gagggtggta tctctaatat
     4921 gcgttattca ataagcgata ctgcaaaata cggggatata accagaggac cgagggtaat
     4981 aaacgatacc gttagacagg agatgaagcg tatactcaag gagattcagt cgggcgagtt
     5041 tgcacgggag tggataattg aaaatagagc tggtcgtccg gtttataatg cacttctaaa
     5101 aaagggtgag agtcaccaga ttgaggaggt tgggcggagg ctcagggaga tgatgagttc
     5161 tctctttaag cagaaactcg ttgataagac gaagaactga agctgtcagc catcggccgt
     5221 aagctgtcag caaaatcttt tactgatggc tgatggctaa acacttacaa tgataaaacc
     5281 gagaagcata atagccgttg aaggtcttcc gtatattggt atttccgcat ttcttgcatg
     5341 gatcacggcg ctctttggtt ttgagtcatt atcaattatt cttgcagccg tcactgtttt
     5401 tatcatatac ttcttcagag atcctgagag gaaaacccct gaagtcccat tatctatagt
     5461 ttctcctgct gacggaaagg ttgtagagat cgataacgct cttgaagaga attttttaaa
     5521 tctcgagatg aaacggatag gaattttctt atccataact gactgccatg taaacaggtt
     5581 tccattaact ggtaaggttt taaacactaa gtacacccca gggcagtttc atatagctga
     5641 cctaaagcgt tcatcaaaag agaacgaaag gcttgccact cttatcgaaa gtgatgataa
     5701 ggaacaaatc gtcgttgttc agattgcggg gtttatagcg aggaggattg tttcttatgt
     5761 ttcagtcggg gatctgcttc aaaaaggcga aagatttggt atgattaaat tcggttcaag
     5821 ggtagatatc tatcttcctc caagttctga agtctcaata acactcggag ataaggtaag
     5881 aggtggtgag acaataatag gatttctgag aggaaaaggc gaatgaagat tcgaacatcg
     5941 tccgttttta ctaacgacag taaaaggaaa aggtcaaagc gtcaaagaat gttgccagtt
     6001 ttgcctaatc ttttcaccac tggtagcctt ttttgtggac tcctttcgat tatgacctcg
     6061 attcagatag cttcattaaa cgaaattcag gctagcgatt cggaatggtt ttatagaaaa
     6121 ttctggtggg cagctgcttt tattgggatc gctgggcttc ttgacctatt tgatggaagg
     6181 attgcaagga ttctcaaatc tcagagcaac ttcggcttaa cttatgattc tctttcagac
     6241 ctggtttcat ttggtgttgc tccgggtgtt ttgatttatg tttttaccct tatgcaatct
     6301 ggaaagttgg ggttaatggt agccctgttt tatatagttt gtacagccct aaggcttgcg
     6361 aggtttaatg tacagtcagg aaatcgtgaa aaatatgcct ttaccgggct tccaagccct
     6421 gttgcagctg ggcttatgtt ctctccgatt cttctttttt ctgagtttca aattacttct
     6481 gatgatagga tgatgtggtt ttatctcatt ttagcaccgt ttgtgggtct ccttatggta
     6541 agcgatgtcc catactggaa aggtttgaga ttagggtggg caaagccttt taatgtgctt
     6601 gtagttgccg caatcatatt cacagcagtt attacaaatc ctgaaattat ggttcttttg
     6661 gtagtttatc tatactcatt aatcgggttg cttttataca taatcagtca gtttaaggaa
     6721 aagcccaagg tcatcgagga gacagagccc gaagaagaga ctccttgagg gcaatttaaa
     6781 tcttttctta ttaaaagcct aactcatagc aaacatttat aattaggagg tttttatgcg
     6841 atatttcttt aactattccg agtgcaatat acgaggaatg gtaataaaag tccttgcctc
     6901 tggtatactg atactcacac ttatggtagg ggataaaata atggctgctg agagagaaaa
     6961 agctgcaaaa tatattgaaa agggtctaga tggtggaatc ggcgttgtga agtatcaact
     7021 tgaaaatggc cttacagtca ttttagagga aaaccattcc gcatcggttg ttgctgtaaa
     7081 cgtttgggtt gaggttggaa gcgcatgcga acaggatggt gaatacggtc ttgcacatgt
     7141 gcatgaacat atggttttta aggggacaga aaaaagggga gttggagaga tagcgagggt
     7201 aattgaggga gatggtggcg atattaatgc atatacctct tttgatgaaa ctgtatatta
     7261 cgttgtgata gcaagtaggt tcctcgatac agcgcttgat gtgctttcag atgcgatgga
     7321 aaattcaaag tttgaccctg atgagttgaa taaagagctt gaggttgttc tcgaagagat
     7381 caggcgtggc gaggatacac cttcaagaaa gctcagcgaa agacttttct ccgaggctta
     7441 tactgttcat ccatatggaa gaccagttat agggagcgac gagagtgtga gaaacttcac
     7501 cagggaaagg gtagttaatt tctacaagaa gtggtactcc ccgaataata tggtgcttgt
     7561 actagtaggt gatttcgacg ctcgcaaggt tattccgaag attcaagaaa cctttggagc
     7621 tttacaggag aggtcgattc ccgggtgtgt tatatccgat gagccagctc agaaggaatt
     7681 aagggcgttt gtttttgata agaatatcca ggaaggttac ttttctctag catttcatac
     7741 accaagtgtg aagcatgagg atgcccctgt gcttgatgtg atttcaagta tccttggagg
     7801 cggtgagagt tctaggcttt acagaagggt taaagaagaa aaaggcctcg tgaataacat
     7861 atatgcctat tcctttacac ctaagtctat cgggcttttt aatgttggcg ggacacttga
     7921 ccctgagaag gctagggatg cgttctctga gattataaaa gaaacgtatc ggctaaaata
     7981 cgagcctgta agttttgagg agctttcaag ggctaaggtg aacattgaaa gtgattctat
     8041 atatactaaa gaaaccatgc agggacaggc acaaaagctc ggctactatg aggtagaggc
     8101 gggagattac aggctagaga gtgattattt acagagggta tctcaggtga cacaggaaga
     8161 cataattcga gtggcgagaa ggtattttaa taacccgaac ctaactgctg gatttcttct
     8221 ccctacagga aaagtgacga t
//
LOCUS       DATDRL010000219         5475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3739105, whole genome shotgun sequence.
ACCESSION   DATDRL010000219 DATDRL010000000
VERSION     DATDRL010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3739105"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..2050)
                     /locus_tag="VI935_07535"
     CDS             complement(185..2050)
                     /locus_tag="VI935_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HLE25487.1"
                     /translation="MREEQSNKGMSRRDFVNMVTAGAVAAGAAGFIDSAKAESAAKEE
                     IIIDRDIWVPMRDGVLLATDIYRPSDNEKHPILVHRLPYDKSYAWIAAGLMFNPLEAV
                     ERGYVVVIQDTRGRHNSEGVWNPFFHEAEDGYDTVEWAASQPWSDGNVGIYGSSYMGV
                     TTWQTVITNPPHLKAAIAYLTGSNYHNGWTYSGGAFELGFNLWWTWFLAGDTLMRLKL
                     SPDELNQALAEWDKLNTGFIEAAKYLPLKNQPAFQKGVATYWREWLEHPSYDGYWEKI
                     DINKRASNIKVPVLHITGWYDNFLRGHLDIYKSIKEKAAKDVQESMRFIIGPWDHGAY
                     LSLNLTSAGERSFGAQAFSGPFFVRDLGYQWFDSFLKGEKTGVNKLPRVRYFDLGKRN
                     WQESEEWPPKHTLTRFYFHSQGKANTRFGDGELSTIAPEQELPNSYVYDPVNPVPSRG
                     GRTLMPTYGPGGIQDQSKVEERDDVLIYTSPQLASSVSVAGPVKVTLYASSSAPDTDF
                     TAKLVDVEPDGYCANVAEGIVRARYRNSMAKEDFMEPGKTYEFKIDLWDVAYTFAPGH
                     RIRVEISSSNFPRYDRNLNTNKPMAEAAESDMKVATQRVFHNTDYPSHIVLPVVA"
     gene            2618..2983
                     /locus_tag="VI935_07540"
     CDS             2618..2983
                     /locus_tag="VI935_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25488.1"
                     /translation="MRRYRSSLIVAFVFAAMVVIALAPPAKSGTNGDGSCLEFCEEQA
                     ICMCAKILGGSLRLTKCNIGAECVDTVNPGSHCECDIDPLCALWEKDVVVQSAPPGNP
                     VAPCEVVWSGDPCFGCWDY"
     gene            complement(4233..4309)
                     /locus_tag="VI935_07545"
     tRNA            complement(4233..4309)
                     /locus_tag="VI935_07545"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4273..4275),aa:Pro,seq:cgg)
     gene            complement(4480..5100)
                     /locus_tag="VI935_07550"
     CDS             complement(4480..5100)
                     /locus_tag="VI935_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLE25489.1"
                     /translation="MDNHYHLLIETQDGNLSLGMRQLNGIYTQRFNKRHGRVGHLFQG
                     RFKAILVDRENYLLELCRYVVLNPVRAGMVRSSGRYKWSSYRATAGTEKISSFLTTAS
                     VLSQCGRSRKVAQEKYKTFVEEAIGKPGPWGKLRGQILLGNDTFVASLEPYIKKVREV
                     EDVPRGQRFVDRPGLEELLRDMEYQPNVVRDNLIREAHWKHGYTLV"
BASE COUNT         1475 a         1315 c         1073 g         1612 t
ORIGIN      
        1 ttcctgcata actttctcct tttgttccta ttataaaagc attaatttat gaatctaaaa
       61 ctgaactacc tggtaaattt aagaatatca atcaagttat cctaaatctt gattcttatc
      121 ttgtggaatt tcgataactc ctttatgatt catctcaaat cttcttaata acatactcta
      181 ttccttacgc cacaaccgga agaacaatat gtgatggata atcagtatta tgaaagaccc
      241 gctgtgttgc gactttcata tcagactctg ccgcttcggc catgggctta ttggtattca
      301 gattgcggtc ataacgggga aagttactgc tgctgatttc caccctgatt ctatgaccgg
      361 gggcaaaagt ataagctaca tcccacaaat caatcttaaa ctcataggtt tttccaggct
      421 ccatgaagtc ttcttttgcc atagagtttc tgtaccgggc gcgaactatg ccctcagcta
      481 catttgcaca gtatccatca ggttcaacat ccacaagctt tgctgtaaag tcagtatcag
      541 gtgcagaact ggaagcataa agcgttacct taaccggacc ggcaacggac acacttgatg
      601 caagctgggg cgatgtataa atgagaacat catcccgttc ttcgactttg gactggtcct
      661 gaatccctcc gggtccgtaa gtgggcatca aggttcttcc accacggctt ggcaccggat
      721 taaccgggtc gtaaacataa ctattaggca gttcttgttc gggagcaatg gttgatagct
      781 caccgtcccc aaaccgagta ttggctttcc cctggctgtg gaaataaaac cttgtaagtg
      841 tatgcttcgg aggccattct tctgactcct gccaattcct tttccccaaa tcaaaatatc
      901 ttacacgcgg aagcttattt acccctgttt tttctccttt tagaaaactg tcaaaccact
      961 gatagcccaa atcgcgcacg aaaaagggac cactgaatgc ctgtgcacca aatgatcttt
     1021 cacccgcact agtgagattt aaagagagat aagccccatg atcccacggc ccgattataa
     1081 accgcatact ttcttgaaca tcttttgcag ctttttcttt aatggattta tatatatcta
     1141 aatgtccccg gagaaaatta tcataccatc ccgttatgtg aagaaccgga actttaatat
     1201 tgctcgctcg tttatttata tctatttttt cccagtaccc gtcatatgag ggatgttcga
     1261 gccactctct ccaatacgtt gcaacacctt tttgaaatgc cggttgattc ttcaatggaa
     1321 gatactttgc agcttcaatg aaacctgtat tcaacttatc ccactccgct agagcttgat
     1381 ttagctcatc cggagaaagc ttaagccgca ttaaagtatc acctgccagg aaccaagtcc
     1441 accagaggtt gaatccaagc tcaaacgctc cgccgctgta tgtccaccca ttatgataat
     1501 tagagcccgt gagatatgct atagcggctt tgagatgagg gggatttgtt ataacagttt
     1561 gccaggttgt aactcccata tacgaggacc cgtaaattcc gacattgcca tcgctccagg
     1621 gctggcttgc tgcccactcc acagtgtcat agccgtcttc tgcctcatgg aaaaagggat
     1681 tccacacacc ctccgagtta tgccgtccac gagtatcttg aataacaact acataccctc
     1741 gttctacggc ttccagagga ttaaacataa ggcctgcggc aatccaagca taggatttgt
     1801 catagggaag cctgtgaacc agaatcgggt gtttctcatt atccgatggc cggtaaatat
     1861 cagtggcaag cagcacgcca tcccgcattg gaacccaaat atccctgtca atgataatct
     1921 cctcttttgc ggcgctctcc gcttttgcgc tgtcgatgaa gcctgcagcg cccgcagcga
     1981 cagcacctgc agtgaccata ttcacgaagt cacggcgcga catccctttg ttgctttgtt
     2041 cttctctcat atccccctcc ttcggtggtt gcggaaatcc ttacacctta ttttccttat
     2101 cccattttat gcacgaactt tttactttgt tattctctca caaagagcag cctcagtcaa
     2161 gaaaaaaggg gtcgagtctt ttgttgatat attcttttat ttggtattcg aaatctcaaa
     2221 attatttcta aggtattatt atgctcactg gttttttatc ctatgattct cgaatctacc
     2281 tctaattaga aattagtcaa caaaagattc gaccctatat atacccctag tcagtagggt
     2341 tgtaatgctc cccttttgtc aagcccgctt taagttggaa tcataaagga taaatgattc
     2401 ctggtaattc tcattagctc cttcccctat acatccaata agcctatgtc taattcgacc
     2461 tacacagcta cattaggtta cagcccataa ttcatctctc caatcaaata acgtgacatt
     2521 atcattttgc aacaacacct gcaatcaact aatgttgact ttggatacga tatatgttat
     2581 ccattgaatt aaatctaaga gaaggggggg taggagaatg agaagatacc gtagttcttt
     2641 aatagtcgca ttcgtatttg ctgcaatggt tgtaattgcc ttggcacccc ctgccaagag
     2701 cggaacgaac ggcgacggta gttgtcttga gttttgcgaa gaacaggcca tttgcatgtg
     2761 cgctaagata ctaggaggga gtctgcgcct tacaaagtgt aacattggag ccgagtgtgt
     2821 agacactgtt aatccaggga gccactgtga atgcgatatc gacccccttt gcgccctttg
     2881 ggaaaaggac gttgtggttc agtcagcgcc tcccggaaac cctgtggccc cgtgtgaggt
     2941 tgtctggagt ggcgatccat gctttgggtg ttgggactac tagacaatac cgtctaccgc
     3001 agagtgctgg ccgacggcga tatgggatga caaccaaggc ttgtgccaag tcacagacat
     3061 atatagccaa gtcccgttgg acttttcaca agcgggcttt atctacggaa acgccgaata
     3121 tttcgtaggt gccctccttg cctccgggat ggaccccccc caggctgtcc cttactcgag
     3181 gaaccaaata tcttagtagg taatctcagc gcgcggagat tggggtcagg tcttgaatcg
     3241 tgcattataa atcaatcttg aggaaaaagg gggctgacac ctttttattc tctgtgacat
     3301 gtcgaattcg aggctattta catttactta cgttacagtt gacgacctcc ttcccttagg
     3361 cttcaccagg ttcgttacct cccctgctgc taagttcagt atagacccga tggttagtct
     3421 ttggtcgtgg tgcctttcgc atgacagctt acttgtatca gcttgcttgg cgcaccgaag
     3481 atgcgacact gagactggaa gcagagccag gcttttgtta ttttgttatt atcttcatct
     3541 atcccacgct ccgtcaaaaa actcttaggg gaaaaagggg ccgagtcttt tgttgatata
     3601 ttcttttatt tggttttcga aatctcaaaa tatttttaag gtattattat gctcactgtt
     3661 tttttccata ctatgattct cgaatctacc tctaattaga aattagtcaa caaaagattt
     3721 gacccctata catgacccct atacatatac gtttgacatt atagaagtaa aagaaaatgt
     3781 gggagaagat tttaagtgaa gaaaagttga attttgagcc ctgaccccca tagttttccc
     3841 tttttatttg gtgaatcata acatcaattc atgcttaaaa ccgaagacta gctttggtat
     3901 tcttaaactc ctatggtaac ccttacaaaa ggcatgactc actaaagcaa atatttggac
     3961 ttgccttaaa acgtacagaa atcaaaagtc ttagattccc tggtctgaga cccatagcgg
     4021 ctacccgtat gatagagagt ggagctaata ttgtagtagt cagttgaatc cttggacact
     4081 ctgatttaat gaggactatg agatattctc atcccgatga ttctctcaag gaagcgatag
     4141 agaggcttac aaatttcatc ctaaaccgta gcaatttccg tagcaatgta aataaggaga
     4201 aaacgctatg agtcgtaagt tcctgaaatg aatggtcggg gtggtgggac ttgaacccac
     4261 ggcctctgcg tcccgaacgc aacgctctag ccaactgagc tacaccccgt gtttatttag
     4321 attattctgg ttgaaggata tttgtttcac ccttaaaaag ttttttaaac ctctccgcat
     4381 caggaaatag gttaatgggg tcaggtcttt aatcgtgcac tatctccgat ttgctatcct
     4441 gcttaccgta gtgtagtgct gccccactat ctctgcaaat tacacaagcg tatacccgtg
     4501 cttccaatga gcctcacgaa tcaaattatc tcttactaca tttggttgat actccatatc
     4561 ccttaatagc tcttctaacc caggcctatc tacaaatctc tgtcctctcg gcacatcttc
     4621 cacttcccgt accttcttaa tataaggctc aagacttgcg acaaaggtgt cgttccccaa
     4681 cagaatctgt cctcttaact taccccacgg tcctggctta ccaattgcct cctccacaaa
     4741 cgttttatat ttctcttgcg ctaccttccg acttctgcca cactgggaca gtaccgaagc
     4801 cgtggtcaaa aaagacgaaa ttttttccgt ccctgctgtt gcacgataac tactccattt
     4861 gtaccgccca gatgacctca ccatacctgc acgcacagga ttcaaaacca catagcggca
     4921 aagttcaaga agataattct ctctgtctac caaaattgct ttaaacctcc cctgaaacaa
     4981 atgccctaca cgcccatgtc gtttgttgaa cctctgagta tatatgccat ttaactggcg
     5041 catacccagt gaaaggttcc cgtcctgagt ttcaattaag agatggtagt gattgtccat
     5101 caagcaatag gcatgtcaga gccaattgaa gcgatccact atgaaaccca ataattccag
     5161 gaagacctta cggtcttcat cgtcctcata gataggctgg cgtgcattgc cacggcttgt
     5221 gatgtggtag atagcacctg gatactcgat tcggagaggt cgagccacag gtagaaatta
     5281 tagatcaaaa atcttatatt tcaagacctg accctatttt tcgtttataa taaaaataaa
     5341 ggatagggag aatccttgat aaatcgaagg gtcacacttt tttgatttct atttccaact
     5401 tctctctatt aaacgtgatg gaaaatttat caaaaaatcc ttcctgacct aagatggctg
     5461 aaaatccaac atttt
//
LOCUS       DATDRL010000220         2648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2113794, whole genome shotgun sequence.
ACCESSION   DATDRL010000220 DATDRL010000000
VERSION     DATDRL010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113794"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..912)
                     /locus_tag="VI935_07555"
     CDS             complement(157..912)
                     /locus_tag="VI935_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25490.1"
                     /translation="MPEATSFDSIDALCQQARANFRTHSSRKQKEIDMVLTLCFEVVS
                     SISKLKTPDEWKDSREEATLAVLFQSIETVLAMHYLSESGFWDNALVLKRNFSELLLI
                     AIAIGFDQQCFIDWKHGRANFDSFERIYKRVEQSSNVPEIEKSLLPHLKRYWSESSQL
                     FSHNMSRKSIRTFVKDGQIHFEPKSASADFQEKRMNTIRNMLLDVISILLGIFQYDKR
                     TFERRADFPEAPGIISRCNECFQNHVWKSEKTT"
     gene            complement(1035..1943)
                     /locus_tag="VI935_07560"
     CDS             complement(1035..1943)
                     /locus_tag="VI935_07560"
                     /inference="COORDINATES: protein motif:HMM:NF037930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25491.1"
                     /translation="MPSLIDELQQLAVDGGVSATELLQKCLIVSTKLGFNDFADWARQ
                     ELDGYKDTAVPDYRIIHGDPQVFDPYRGYQPLHCSDPKFKKAISRMHFNQPIGEIEHD
                     LRHSEKSGSDAFQITYAPDFEKKLMDGIEFNLRPSLHVSASQLRKIIDGVRKVVLEWS
                     LKLEADGIVGEGMGFSKDEKKKASTITYNIQNYIRGEFKGSQIQVDTTSSNQNLTTNQ
                     FNIEEVKQLVGTLNASLDELELSQNQKEELMSEIKTLDSQAGSPKPKQGIIRESLLSI
                     RTILEAASGNLVASGILNQIGALFGP"
BASE COUNT          687 a          567 c          565 g          829 t
ORIGIN      
        1 tatgatggaa aagaggttgt actgaaacca ggagatgtca ttgcgatacc ttcaaatgct
       61 gttcattcag cttttactgg agaaaaatcc tgcagggcag ttgatgcatg gtcgcccgtc
      121 aggaaaaaat tccttaataa atccgttttt cctattttag gtcgttttct cgctcttcca
      181 tacatggttt tgaaagcact cgttacatcg agagatgatt ccgggtgctt cagggaaatc
      241 cgctcgcctc tcaaaagtgc gtttgtcata ttgaaatatg cccagcagta ttgagataac
      301 atcgaggagc atattcctga tcgtgttcat ccgcttttcc tgaaagtccg ccgaagccga
      361 tttcggctca aaatgaatct gcccgtcttt gacaaatgtt cggatgctct tgcggctcat
      421 gttatgtgag aaaagttgcg aggattcaga ccaataacgt ttcagatgag gcaataggga
      481 tttctcaatc tcaggaacat tggagctctg ttctacccgc ttgtagatcc tttcgaaaga
      541 atcaaagttg gcccttccgt gcttccagtc gataaagcac tgctggtcga accctattgc
      601 tatcgctatc aaaagtagtt cggagaaatt tctcttgagt actaacgcat tatcccagaa
      661 gccgctctct gataagtaat gcatcgcaag gacggtctcg atcgactgaa atagaacggc
      721 gagggtcgct tcttccctgc tgtccttcca ctcgtcgggg gtcttgagct tactgatgct
      781 actgactact tcaaaacaga gggttaaaac catatcgatc tctttttgtt ttcgagaaga
      841 atgagtccgg aaattggccc ttgcctgttg gcaaagagca tctatactgt cgaaacttgt
      901 ggcttcgggc atgaacgttt tctctggtat tgccgcctaa tgcggcggtt gagcgggggc
      961 ccatcgatta agctcacctg caagcagtgc gccagcaccg caagtcaggt ggagcgaatt
     1021 gttagctctc attttcatgg gccgaacagc gcgccgattt gattgagaat tccactcgcg
     1081 acaaggtttc cgctggccgc ttcaaggatt gttctaattg atagcaatga ctcccggatt
     1141 atcccttgtt ttggtttcgg cgaaccagcc tgagagtcaa gagtttttat ctcagacatg
     1201 agttcttctt tttgattttg actcagttct agttcatcga gcgaagcatt gagcgtacca
     1261 actagttgtt tgacctcttc aatattaaat tggttggtcg tcagattctg gttacttgat
     1321 gtcgtatcga cttggatctg tgacccctta aactctccgc gaatataatt ttgaatattg
     1381 taggtaatgg tagaggcctt tttcttttcg tctttagaga agcccatacc ttcgcctact
     1441 atgccgtcgg cctcaagctt cagtgaccac tccaacacta cttttctgac gccgtcgatt
     1501 atttttctca attgggacgc gctgacgtgt aatgatgggc gcaaattgaa ttcgattccg
     1561 tccattagtt ttttctcaaa gtcaggagcg tatgttattt ggaacgcatc agaaccagac
     1621 ttttcagaat gtcttaaatc atgctctatc tcgccaatag gctgattgaa atgcattcgc
     1681 gatattgcct tcttgaattt cggatcagag cagtgcagag gttgataacc tcgatacgga
     1741 tcgaatacct ggggatcgcc gtgaatgatt ctgtagtcag gaactgccgt gtctttgtag
     1801 ccatcgagtt cttgacgtgc ccaatctgca aaatcgttga atcctagttt tgtcgaaaca
     1861 attagacatt tctgcaggag ttcggtagct gatacaccgc catcgacggc cagttgttgg
     1921 agttcgtcta taagtgatgg catttggttt tctgtcctcc gcttacatgg gagctaacat
     1981 tcacttttat acttattcta tgttcagcca tagaatacaa agataatatc gaaaaagtca
     2041 agaaaatcta cccaaaattt gttgaaaagc atgttgttat tccgcataat acttctattt
     2101 taaatatcct aaaaatagac attgattttt ggggcaaaat cacacgagtt gttggctcac
     2161 aaaggggcat gaaaattaac ccaccctcac cttaatcctc tccctgaggg agaggaagaa
     2221 aagaggggaa ggcaaatgct aaagtccttt cggttttctc tgtgcttctt taaccattca
     2281 aaaaactgcc tccgttttaa gccaagtaat tccatagctt attctctgct aagctcattg
     2341 ttgttatacc tattcaaaat cattttcacc tgttcttccg tcagtctttt atgaatttgt
     2401 tccgtcatac tacttttcct cccttatgtt taatttaaaa tactctttat caaattacca
     2461 taaaggagtg cggttttatt ttttagctaa caaaacctga taaaaatctt gacaaagcat
     2521 gaggttatct tgtaatttag tatattcaaa attatttgga gatgctggat gttcgatagt
     2581 ctgagtgata aattcagtgc tgtaataaag aaggtaaggg gccttgcaaa tctgtcagag
     2641 gcaaatat
//
LOCUS       DATDRL010000221         3027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1212238, whole genome shotgun sequence.
ACCESSION   DATDRL010000221 DATDRL010000000
VERSION     DATDRL010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212238"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VI935_07565"
     CDS             <1..919
                     /locus_tag="VI935_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLE25492.1"
                     /translation="TRKIIRFLREKGYKTVVVRHPMPYGNLKKQICQRFSKYEDLEKN
                     DCTLEEIEEYEPHIREGVVVYAGVDYMEILKEAEKEANIIIWDGGNNDLPFFKPDLHI
                     VIADARRAGHEVSYYPGQANLMMADIVIINKVNTAKKKDVQLIKENVKRLNPKAKIIM
                     ADMPKYVDNPQLVMKKRVLVVEDGPTLTHGGLSYGAGALEAKRLKAKIIDPRPYATRS
                     IKEIYKRHPHIGKVLPAIGYGKAQMEDLEKTINRVPCDTVLIGTPIDLGKYIRVNKPY
                     ARVTYGIKEIGKPSLEGIISGLILHSSHI"
     gene            1310..2119
                     /locus_tag="VI935_07570"
     CDS             1310..2119
                     /locus_tag="VI935_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HLE25493.1"
                     /translation="MDSFQNLNPVLQALIATCFTWGMTALGASIVFMAKDVSKKILDG
                     MLGFSGGIMIAASFWSLLAPAIEMSEGKDVPTWFPALTGFLIGAIFLWGIDRILPHLH
                     LGFSIEEAEGIKTTWQRSILLVLAITLHNIPEGLAVGVAFGAVVSGFSSATLSAAIAL
                     AIGIGIQNFPEGLAVSAPLRHEGMSRLKSFWYGQLSAIVEPIAGVIGAVAVIVVQPIL
                     PYALSFAAGAMIFVVIEELVPESQRSGNTDLATFGAMIGFAVMMVLDVAFS"
     gene            complement(2239..>3027)
                     /locus_tag="VI935_07575"
     CDS             complement(2239..>3027)
                     /locus_tag="VI935_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25494.1"
                     /translation="VYLMDFGVPDDNDRDLGFEDYIFGYIPSAVEGVLKAASSNSLSL
                     LGYCLGGVFTLLYVTLTDNPVKNIVLVASPVDFSKLGLHYHYWSNVDADRLVNTFGNI
                     PPELIIISFNLIAGIKDPFRYLRKSLGFLQDISNKEYVKRELLINRWLIESPPFPAKA
                     FKQLIIEFFYKNAMVKMDIRMKGKLADLSRIKCPILILSHTADVVSPPASARVLLDLI
                     SSKDTEFLEVSGGDAGHIDIIVGNEGAEFTWPRIASWLKRRSNK"
BASE COUNT          953 a          586 c          651 g          837 t
ORIGIN      
        1 aaccagaaaa ataattagat ttcttaggga aaaaggttat aaaactgtgg tagtaagaca
       61 ccctatgcct tatggtaatt tgaaaaagca gatatgtcag aggttttcca aatacgagga
      121 cttagaaaaa aacgattgca ctcttgaaga aatagaggaa tatgagcctc acataaggga
      181 gggcgtggta gtctatgcag gtgtggacta catggaaata ttaaaagagg ctgaaaagga
      241 agcaaacatt ataatatggg atgggggtaa caacgacttg ccatttttta agcccgacct
      301 tcacatcgtc attgctgatg caagaagagc gggacacgag gtaagttatt atccggggca
      361 agctaacctt atgatggcag acattgttat cataaataaa gtcaacacag caaagaaaaa
      421 agatgtacaa ctcatcaaag aaaacgtgaa aagactaaat ccaaaagcca agataataat
      481 ggctgatatg cctaagtacg tagataatcc acaactagtt atgaaaaaaa gggtactggt
      541 ggtagaagat ggaccaacat taacccatgg aggcttaagc tatggggcag gagcattaga
      601 ggcaaaaagg ttaaaagcaa aaataattga ccctcgtccc tatgctacaa gaagcataaa
      661 agaaatctat aagaggcatc cccatatagg taaagtactt cctgcgatag gctatgggaa
      721 ggcccaaatg gaagacctag agaaaaccat aaacagggta ccgtgtgata cggttcttat
      781 aggaacaccg atagacctcg ggaaatatat cagagttaat aagccgtatg cgagggttac
      841 ttatggtata aaggagatag gaaaaccgag tttagaaggg ataataagtg gacttatttt
      901 acactcatct cacatataga actaacacct tcacaacttg caggcctgtg gaccatattt
      961 tgagaggtct tctttttcaa aaaactactc cacccctagc tctagcacct tttccttttt
     1021 tgagccgtaa cgaaagatag taattccttt gcatccaagc tcataaccca aaatataagc
     1081 tttttgtacc cttctgcctt ggaatcctta gtcatatcaa tacaattaat aagaaaatga
     1141 acggcacctt aaaagctatt tagccagaat actatcggga atcgtacatt gtaaattaag
     1201 taacataata gtgccataaa gggcttagct aagcacaaag aggtgaataa ttttgtggtt
     1261 taatataaaa tagcgatgaa cagaacttaa agagggactc aggatcatga tagattcttt
     1321 tcaaaacctc aaccccgttc tacaggctct tattgcaacg tgctttacat ggggaatgac
     1381 tgctcttggg gcttccatag tgtttatggc taaggatgtt agcaagaaaa tactcgacgg
     1441 aatgctgggg ttttccggcg gaattatgat agccgctagc ttttggtcat tgcttgcacc
     1501 ggctattgag atgtcagaag gcaaagatgt tcccacctgg tttccggctc tgacaggttt
     1561 cctcatcggg gctatcttct tgtggggtat tgatagaata cttccacacc tccacctcgg
     1621 cttttctata gaagaggctg aggggattaa gacaacgtgg cagcggagta tattgcttgt
     1681 tcttgctata acactgcata atatccccga aggtcttgcg gttggagttg cctttggggc
     1741 tgttgtctct ggtttttctt ctgccacttt atcggctgcc atcgccttag ctatcggtat
     1801 cgggattcaa aattttccag aaggactcgc agtctctgcc cctcttcgac acgaggggat
     1861 gtcccgtttg aaaagttttt ggtatgggca gttatccgca atagttgaac ctatcgccgg
     1921 tgttattgga gcagtagctg ttattgttgt acagcccatt ctgccttacg ctttgagttt
     1981 tgcagcaggt gccatgatct ttgttgttat cgaagagctt gtcccggaat ctcagcgtag
     2041 tggtaataca gatttagcca ctttcggggc aatgataggc tttgctgtaa tgatggtact
     2101 tgatgtagcg tttagttaat aaagaaaggt tttgcttttg gaagccaagc aacctaagac
     2161 actattggct cactgactta cttcataaga agctgtagat gtgattgtag gggcatatta
     2221 aaacgactag ataatgcttt acttattaga cctacgtttt agccatgacg caatccgagg
     2281 ccaagtaaat tcagctcctt cgtttcctac aataatgtca atatgtcctg cgtcccctcc
     2341 agagacctca aggaattccg tatctttact tgaaatgagg tcaagaagaa cccttgccga
     2401 tgctggcggg gaaaccacat ccgctgtatg agaaagaatt agaatagggc atttaattct
     2461 ggatagatcc gccaatttcc ctttcattcg aatatccatt ttaaccatgg catttttata
     2521 gaaaaactct ataataagct gcttaaaagc cttggcaggg aaaggaggtg attctatcaa
     2581 ccatctgttg ataagcaatt cccttttcac atattccttg tttgaaatat cttgtaaaaa
     2641 acccagggac ttcctaagat aacgaaatgg gtccttgata ccagcgatca aattaaagct
     2701 tataattatt aattctggag ggatattccc aaacgtatta actaatcgat ctgcgtcaac
     2761 gttactccaa taatggtagt gaagacccag ctttgaaaaa tcaacagggc ttgcaacgag
     2821 aactatattt ttaacagggt tatcagttaa agtcacgtat agcagagtaa aaacgcctcc
     2881 gaggcaatag cccaaaagtg ataatgagtt agaagaagcc gcttttaaaa caccttctac
     2941 tgcagatggt atgtaaccga aaatataatc ctcgaatcct aaatctcgat cgttatcgtc
     3001 aggtactccg aaatccatta ggtaaac
//
LOCUS       DATDRL010000222         4702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1828644, whole genome shotgun sequence.
ACCESSION   DATDRL010000222 DATDRL010000000
VERSION     DATDRL010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828644"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            585..1904
                     /locus_tag="VI935_07580"
     CDS             585..1904
                     /locus_tag="VI935_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLE25495.1"
                     /translation="MDPFFTLNNAHLDNSSSYENLDPHLRKLKTQPYVFESSLLHLFP
                     FQIPGVYTLGGARQIGKTTFLKQWISSLLKKGVPATSIVFLTGELIDDHYRLLHIIKE
                     AFEMMSPKDSFKTIILDEVTYIKEWDKAIKFLADSGLIENCMMVLTGSDLALMKEARM
                     RFPGRRGKHSQVDFHYYPLSFQEYVTLVHGKEENWNISRLYEAFQSYLVHGGFLTAIN
                     DIAIHNKVQPSTFQTYSDWIRGDFLKRGKKEKYLKDFLLAVLKTYGTQITWNSLAHHT
                     EIDHHQTLQEYADIMQSMEALFIQEALVEHKCMAAPKKAKRLFFSDPFIYHAINLWLD
                     PRYSLEKTLTHTETLSSLVETTVINHAKRLYPTYYIKAEGEVDLAYVKGSTFIPIEIK
                     WGKQLRPKDLKQIKKYPQGQIWAHVQDTYHLEGLPVIPLPKALYEFS"
     gene            complement(2133..2438)
                     /locus_tag="VI935_07585"
     CDS             complement(2133..2438)
                     /locus_tag="VI935_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25496.1"
                     /translation="MSSTNADRQRKYKEKRRAKDLSLVQVWVPKDRIVEIKSIAAGMA
                     EEGSRVRDLDLEPSQRQLNFAQFLCDKKGLKINQEVLSSSKKLSEWLNKNKRKSDKI"
     gene            complement(3100..3357)
                     /locus_tag="VI935_07590"
     CDS             complement(3100..3357)
                     /locus_tag="VI935_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25497.1"
                     /translation="MFASRTTMSYGEADVLEEETDKSTLTERRGMGPDKHTRNGEITS
                     GRSIDHAGTNNNLQRLIRLGTTKLRRDDGLAHEFVRAMRGG"
     gene            3654..4124
                     /locus_tag="VI935_07595"
     CDS             3654..4124
                     /locus_tag="VI935_07595"
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HLE25498.1"
                     /translation="MADKIGLTVNREKTHITKITDGFDFIGFRFVKRKSLRSSKDNIY
                     VFPSKRAQQAIRNKLRYVTSRRAPIKPEEFIESIRPVVMGWVNYFRHTNASDAFRGLQ
                     RFINTRFRRYLTYRSKGRGFGWNKYPNKKLYAMGMIYIGSGLIEYPERLVHGLR"
     gene            complement(4511..>4702)
                     /locus_tag="VI935_07600"
     CDS             complement(4511..>4702)
                     /locus_tag="VI935_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25499.1"
                     /translation="YTDAKMAFHLNNKKGSHSSHKGRDQNKQEGRIPSQSKEFYRRRC
                     GTLTTFRTTKTQSFLVERP"
BASE COUNT         1258 a         1037 c          946 g         1461 t
ORIGIN      
        1 gtgaaggcct ccttttcata aaagtcaata gtattttaca gccggaattt tgcatcaaga
       61 aaagaaaagt ttccgtttat tcataataat caacgatgta aaaacatcct ccttttatca
      121 aaatcgttcg tttgtgataa taaaataaga cctctttggg gaaaggaaaa ctgaaaaaat
      181 tacatgttaa ggagagggtt aacaatgaat gaagtgttaa ccctcttttt cttttaccta
      241 tgttctaatt gttgactcca attttggcac attttagaca taacgttatg taaaacgtgt
      301 atcctttcaa gttccatgga ttcttgttct ctcaaagaat ccattttcct taaattgttt
      361 tctagttctt ttaaaaagta ctgaaagttg tgtatggtaa tgtcagacat gatcaagcct
      421 ccttgacagg gttggttgtg tttagcaacg ctcgggtgtt gattgcacct gggcgttgtg
      481 tccatcttac agaagatttc ttttccttac aatggatttt tatgcgtgac tgtttttaga
      541 taaagtatat tgactgctat tgaaagaaga ggtacctttg ctctatggat cctttcttca
      601 cgcttaataa cgctcatctc gataattctt cttcgtatga gaatttggac ccccatttac
      661 ggaaactcaa aactcaacct tatgtttttg aatcctctct cttacatctt tttccctttc
      721 aaataccagg cgtgtatacc cttggaggag caaggcaaat tggaaagact accttcctca
      781 aacaatggat aagttctctt ctcaaaaaag gagttcctgc aacgtctatt gtttttttaa
      841 cgggtgagct tattgatgat cattaccgtc tcctccatat tatcaaagaa gcctttgaaa
      901 tgatgtctcc taaagactcc tttaagacca tcatccttga tgaggtgacc tacattaagg
      961 agtgggataa agccattaag tttttggctg atagtggact cattgaaaac tgtatgatgg
     1021 ttttaacagg ctcggatctt gctctcatga aagaagcaag aatgcgtttt ccaggaagac
     1081 ggggaaaaca ttctcaagtc gatttccatt attatcctct ttcttttcaa gaatatgtca
     1141 ccctcgtcca tggaaaggag gaaaactgga atatttctcg tctttatgag gcctttcaat
     1201 cttatctcgt ccatggaggg tttttaacgg ctattaatga catcgctatt cataataaag
     1261 tccagccttc tacttttcaa acttatagcg attggatccg gggtgacttt cttaaaagag
     1321 gcaagaagga aaaatatctc aaggattttc ttctggctgt tttaaaaacc tatggaacac
     1381 aaataacatg gaactctcta gctcatcata cagagattga tcatcaccaa accctccaag
     1441 aatacgcaga tataatgcaa tctatggagg ccctctttat tcaggaagcg cttgtagaac
     1501 acaaatgtat ggcggctcct aaaaaggcca agcgcctctt tttttccgat ccctttatct
     1561 atcatgccat taacctttgg ttagatccaa ggtatagcct tgaaaaaacg ctcactcaca
     1621 cggagactct ttcctctctc gtagagacga ccgtgattaa tcacgcaaag cgtctttatc
     1681 cgacctacta tatcaaggca gagggagaag ttgacttggc ctatgtaaaa ggatcaacct
     1741 tcatccccat cgaaattaaa tggggtaaac aacttcgtcc caaagatctt aaacaaatca
     1801 aaaaatatcc ccaaggacaa atatgggctc atgttcaaga cacttatcat ttagaaggtc
     1861 ttcctgttat ccctctccct aaggcgctgt atgagttttc atagggatga aacgatttgc
     1921 tcttgagcca taagcctgct caaaaaaatc atttcttcat gaagacgctt taataaatgg
     1981 cgagaatttt gtatatcagg attagtcatg atcaagcctc ctagagtaag gtttggttgt
     2041 gatgagcaat gctcgggtgt ttagattgag gacacctggg cattgcgctt agtctatctc
     2101 attctcacgt tagcgacaat caattctttt ttctaaatct tgtctgattt cctcttattt
     2161 ttgttcaacc actcagaaag tttctttgaa cttgaaagaa cttcctgatt aatttttaat
     2221 cccttcttat cgcacaagaa ctgcgcaaaa ttgagctgcc tttggctcgg ttctaaatca
     2281 agatctcgaa ctcgactccc ctcttctgcc attcccgccg ctatagattt aatctccacg
     2341 attctatcct taggcaccca aacttgaaca agagaaagat ctttggctct ccttttttct
     2401 ttatattttc tttgacggtc tgcgtttgta gagctcattt aattttattc cctgaccgtt
     2461 accgtaacaa cggtattata tgaggagtga aagtcaagta gatacttgaa cgcgcgttgt
     2521 catttttgac aacgcatatt gcgtatgtcg taattctgac gaatcacttc cataaccccc
     2581 tttggtgatt ttcgtggggc gcttaaattt attcggttga aaattcttct ggtttttttc
     2641 tgtctgtctc agcgctttct cttgagatcg tgtctgataa aaaatcttag gagttttgtg
     2701 gattttggtc tgtcgggtag aatcgaggcg cgcaacaacc ttagggtcca gtggatgtat
     2761 cctctccttt cgtagaggtc ctcaatctgg ctcccttcgc tttacgtttc ctcgaagccc
     2821 ccttttcatc ccggacggtc gggtttcccg agtccggttg gcgaccttag ctttccttac
     2881 aaagccttcc cgataaaagc gaagcttaag cgctaactcg catacacccc ttcctctatc
     2941 ggtttactct gtacctcgcc atagaaggcc cactgcctac tcccagaatc tgagttctga
     3001 aaggtgtatg gtttctatga ccgctaagcg ccgagagttc ctttgctcct gttgggtgtt
     3061 gcctttcagg aaatgatgtt cccatcgttt aggaaggagt taccctcccc tcatcgctct
     3121 tacgaactca tgcgccaatc catcgtctct cctcagcttc gttgtaccta atctgatgag
     3181 tctctgcagg ttgttgttag tccctgcgtg atcaatggac cttcccgacg ttatctctcc
     3241 attccttgta tgcttgtctg gacccatacc ccgtcgctct gttagagtgc tcttatccgt
     3301 ttcttcctct aacacatcgg cctcaccata cgacatagtg gttcggctcg cgaacattcc
     3361 ctacaatgca atttccataa gggtcgcttt tcggggctgc agtcattctc tcatgttcag
     3421 acctgcatac ttgctcggcc ttccgattgc acctacctga gggattatct ctctcggcag
     3481 ccagacctta tacaccacgc aatgaacaga ttgttacctt tctgaactgt ggtatcgcta
     3541 cgtgactgaa tcggacaatt gtcacggctg gaattccacc agctggattg gagccttgtc
     3601 ggccgctacc gtgccgcaaa ggacctgctc ctgtgctaca aatgtttacg gagatggcag
     3661 ataagatagg tttaactgtt aatcgggaaa agacgcacat aacgaagata acggatggat
     3721 ttgatttcat cggttttcgc tttgtgaaac gcaaaagcct gagaagcagt aaggacaaca
     3781 tctatgtctt cccatcgaag cgagcacaac aagccattcg gaacaaactg agatatgtga
     3841 cgtcacgaag agcgccaata aagcctgagg aattcataga gagcattcga cctgtggtca
     3901 tgggatgggt aaactatttc cggcacacga atgccagtga tgcctttcga gggttacagc
     3961 gctttattaa cacccgcttt cgaagatact taacctatag aagcaaaggc cgagggtttg
     4021 gatggaataa atatccgaat aagaaactct acgcaatggg aatgatctac attggtagtg
     4081 ggcttattga gtatccagaa agacttgtgc atggtttacg atgaagactg tcgggccgcc
     4141 gtattcggga aaactgaacg tacggtggga tgagaagggg atgggtaccc aaccacggag
     4201 gagctagtga gcgtgagcaa attaattgat gcataagtgg gaggatttta atcaaaataa
     4261 atgagggacg cataaaaaaa cccatcaaag aatttgatta aaaactcttg atgggtttta
     4321 ctctttttta atacagaaga agagcatatc aatgtcccat gattttctta aagatacaag
     4381 ctttcattta caactggatg ccattgatca gaatttggct catcaaacac aggcaagagg
     4441 ctgtcctcta tgcggacata aactccattg tgccaattat cctcgtagcc ctatgggact
     4501 tcctcccccg ttatggtctt tctacgagaa acgattgagt ttttgttgtg cgaaatgtcg
     4561 taagcgtacc acacctcctt cggtaaaatt ctttggattg cgatggtatc ctgccttctt
     4621 gtttattctg gtctctgcct ttatgcgagg aatgagagcc ttttttattg ttcaaatgaa
     4681 acgccatttt ggcatcagtg ta
//
LOCUS       DATDRL010000223        15174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1157362, whole genome shotgun sequence.
ACCESSION   DATDRL010000223 DATDRL010000000
VERSION     DATDRL010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15174
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157362"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="VI935_07605"
     CDS             complement(<1..1215)
                     /locus_tag="VI935_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE25500.1"
                     /translation="MLVKKTMNLQELKNQLKLFLVIFLLIQPYSCAHKSIENQVKADT
                     SKKTEPQSEKYEAYYHFTLSRLNFFRGNFEEAIREIETAEKYDPNSAYIKYNIAVIYM
                     SVNRFTEALNKLEESVRVNPSFAPSHTLLGKIYASSEDPEKKKRGVKELKMAIELDPN
                     DSEAHLFLGIIETESKEYDKAGISFKRVIELIPDDERGYYFLGRPNLEKGDLVESENY
                     YKKALRLNPVYPSALLDLAIIYEKQGKLDESENIYKELVTIFPSSFDAYVRYGNFLFR
                     INRLSEAAIQFKKAQQLDYQNPDLKLRLGLLYLEKEEYEKAIEEFQLILLGNPGDERA
                     KYYLALCYTETGRYDESLKILDQIPKNSALYNDALIQRAYLFEKMGRTDESLKMIKEA
                     LKHDPDNEQIVNY"
     gene            complement(1205..2614)
                     /locus_tag="VI935_07610"
     CDS             complement(1205..2614)
                     /locus_tag="VI935_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HLE25501.1"
                     /translation="MYFLGFLIIFLLNASQSIALKVNYEEEWMGIYSGGDKIGYSHTI
                     YKPKRENLQIFEETKLNMDTLGTKQVVDITSTYLLNKYELQAFEFSMKVGPVQFSAEG
                     KKEGKKLDIQISSVSGKSNITFPIENEPLVSPIIFKWLAEQKPRVGKSYQVTLFDPTL
                     ILTGADAKSLLATLSIEGEEQIKTPYGSFKTYRVKMLFMGSESTYWITKEGEIIKEFS
                     QPGLLALRESKEKVLGETLQSLDIVQKTAISSNVSLDNPRTLKFLRIKIDGIESPKEP
                     NLEDNRNLFKDGIIEVRRSDLSQIIPYEIPYTEEAYKSYIKPTSLIQSDDPKIIEESV
                     EILRGEKDSLRAAKEINNWVYKKLEKAPTVSLPNALDVLQTRRGDCNEHAALFAALSR
                     AAGIPTKIVFGVVYVNGKFYYHAWNEVFLGSWIPVDPTFGQLPADASHIKIVEGDLNR
                     GSEIIRLVGKIKIEILDAS"
     gene            complement(3176..3463)
                     /locus_tag="VI935_07615"
     CDS             complement(3176..3463)
                     /locus_tag="VI935_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLE25502.1"
                     /translation="MIKGKNRYIKFKKRVLARPKVRESFEEGLDVLRLAVSMAELREK
                     RGLTQTQLAAQLHTSPAVISRIENGGNAELKTLQRIAQALHARLKIELVEA"
     gene            3605..4681
                     /gene="mqnC"
                     /locus_tag="VI935_07620"
     CDS             3605..4681
                     /gene="mqnC"
                     /locus_tag="VI935_07620"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HLE25503.1"
                     /translation="MIASEIVNKIDRKGRINPEEGLMLLREADLLELGSLADSVRRRF
                     HPDGIVTFVADTNPNYTNVCDTECLFCAFWRPAGASDAYTLTIDEVLRIMKTSFHNGA
                     TTVLLQGGHNPEIKLDYYIELVRRVIEEVPLLHLHAFSAPEISAIANYSHISTKEVLE
                     KLWDAGLRTIPGGGAEILSNKVRKKISPLKIDADKWIQVTKEAHEIGFKSTATMMFGH
                     VEEDVDIIEHLERIRSLQDETGGFLAFIPWTFKPRNTFLEKKIPREISGDRYLRVLSL
                     SRIYLDNFNQVQGSWFTQGKKIGSISLHYGASDLGGTLYDENVLGCAENKLRATMTEL
                     VHMIKSAGFKPAQRDTFYKVIRYF"
     gene            4839..5363
                     /locus_tag="VI935_07625"
     CDS             4839..5363
                     /locus_tag="VI935_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25504.1"
                     /translation="MLKKVYVPSLFCTFILIGIILILSCGAQRTYQTKSPPGGFASYV
                     IVEIPDFKTSVNPVPPDVIWKIPNEIAEKLRKELLFTGVSRSPVDLSDRVLILDGTMV
                     GYTPTIWYKRIVNTGRVIANVRFIDKSENKVIAESSFEGTVEGGYFGGGMHFANVRLA
                     DEVVQYIKRNFSPQ"
     gene            complement(5368..5748)
                     /locus_tag="VI935_07630"
     CDS             complement(5368..5748)
                     /locus_tag="VI935_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25505.1"
                     /translation="MDIESLVEKKIHISNLDDFLASALLECQEKPIKLSIQKEGIEGY
                     VSVNLLKSEENMEEDGIDFELSPCECINFDSMEISDFLYKPVFSSSAYEFFDFVFMFF
                     SSIECLVDFTGDAWKVKILKTEYP"
     gene            complement(6237..7658)
                     /locus_tag="VI935_07635"
     CDS             complement(6237..7658)
                     /locus_tag="VI935_07635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLE25506.1"
                     /translation="MWLDEAISVWFANESLGNIIIKQTSADVHPPFYYIVLSLWINVF
                     GNGEFEVRLLSAIFGILSIPLLYLIVKDLFGNLPALISALMLALSPFHVYYSQEARMY
                     SILTFFVLLSIFFMAKMLCIGEEIKLTKKTIFYSIGYVISTVAALYCHNVALLLPLAQ
                     NLFFIIFWNRHRPFFKFWTLSLLIIITLWAPWTSFFFEQSSSVGKSFYTPQLTLESIF
                     NLFATFNNGPTYWLFNWIDVGNFSLIHGRLVLVTIIFFSLLFLAGIIYGRRNPRSLVL
                     LLLISFVPIGTQLLISLKHSILATQTLIWASGPYIVLIALGISSIKRKWVFALTIVLI
                     SVLNSASLYKYYAEFKKERWDLAAEYVSQNSGKEDLVLFSSALGQIPFEYYFDRYNLM
                     LEKHGLPTNFSERKMTIEDVPALEDLIQNHKRVWLIYSHEWFTDPEKLSESTLKKNYC
                     LSKSKDFKSGQSNLSCYLFERCK"
     gene            complement(7911..9302)
                     /locus_tag="VI935_07640"
     CDS             complement(7911..9302)
                     /locus_tag="VI935_07640"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HLE25507.1"
                     /translation="MKKKWAATFLILAIILSVENNNTAQANVAVGFNLFFNTLSPYGN
                     WVSVPRYGNVWYPTGVGPHWRPYTDGRWVWSDYGWTWVSYEPWGWAPYHYGRWIFLDY
                     YGWVWIPGNIWAPAWVTWYTSPGYIGWAPLPPDDNFFLGINIGFTNYNYYNNYNYYTP
                     PSHCVFVPSHKFLHHHVHSAVVPQSHNVKIFKEATHINNARIVNNNVVNRGPDVGFVE
                     RVSRTKVEKVNLVEKDIDTDRIVKTGVNVNKLDGDNYNIFRPDLVKKNKGAALPNAEA
                     PKKALQIENTHNRFSSYNKLQNLPNRNYPKVKKESLKNSLTIRDDNDHSYSNNKVKIK
                     NEERYQKQENIKDAPDQQYNAHSENKKRNSLKRTHSAYTENRKGYEPNYLHVKRANNN
                     SKRFPYQNNADKPKQKDYPINSNKRWITSQEDNINQFQNQDRQPKARYREQNNVELKS
                     PKMSKRSKRNESN"
     gene            complement(9621..9815)
                     /locus_tag="VI935_07645"
     CDS             complement(9621..9815)
                     /locus_tag="VI935_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25508.1"
                     /translation="MDGKNLTPQNAPGSMIGNLGGVLLPPGMPFFTFIGQPPSYRVSA
                     YKDIPFVIAMTAPLSVIPSD"
     gene            9955..11136
                     /locus_tag="VI935_07650"
     CDS             9955..11136
                     /locus_tag="VI935_07650"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HLE25509.1"
                     /translation="MINAGVGYSLNENGEQAAKEAARQAIENGDITKADFAVVFCSFP
                     HRGSYKDILKSVCQITGTTNITGCSGIGVLTNYGEVEAKPGVAVLAVSSDGMHATSFL
                     VHHTDDGGMKAGLEIGGRLIPIKDENALLAILPDPFHIHPELLMRGIESRIGEIQIVG
                     ASASEHPAFHETYEFYGESVVSGAISGFMIQGSFTHRIGITQGCQPVGIPCIITKSDQ
                     NLIFELDGQPAFEVLKKQVPKRILENPRKLLRLLFVGFSPDPKETEIIGGEYLVRNLI
                     GINPNTGVIGVAENVREGQIMTFTVRHPIMAREDLKQMLERLASSRNTQKRFKFGFYF
                     NCCARGSSLYGYEGIDTAYITHALGEIPIIGFFGNSEIAPLKGINRLFTYTGVLVLIS
                     E"
     gene            11772..12551
                     /locus_tag="VI935_07655"
     CDS             11772..12551
                     /locus_tag="VI935_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HLE25510.1"
                     /translation="MGYKFFELSEEDSVGVVRLNRPPVNAFNLDMAREFDALLDDVAK
                     IEGIKSLVVTGNKKIFSAGADIGLIRSSEWSYLKELVEIGQRAFQKMENMTKIVIAVI
                     EGHCMGGAMEFALACDRRFMAIGEAKIGLPEVKLGLMPAWGTTYRLPCLIGKSKALDL
                     MVRGTLLNAEEAKAIRLVDEIYPQVEVSTKAMDYAKSIAIGATFAIGKIKSCINKSFD
                     LTFKESMELECNSQGELFKTQDYGEGTQAFLEKRSPRFVGK"
     gene            complement(12625..14100)
                     /locus_tag="VI935_07660"
     CDS             complement(12625..14100)
                     /locus_tag="VI935_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLE25511.1"
                     /translation="MAKPIRKYGLFIDGKEINSVSGKTYIRENPATEEPFAEIAEAQR
                     EDVDRAVKAAKRAFEKWSHVPSRERAKFIFRLAELLEKHKEHIAMTNTLETGKPIRES
                     RLVELGNSIRTLEYYAGAATKLNGESISVSDDQLTITLKEPVGVAAHIIPWNFPLLLS
                     FWKIGAALAAGCTIVLKPATDTSCGILEFARITAEAGLPEGVFNVIPGKGSLAGQALI
                     EHPDISKIAFTGSTEVGKGVMNTASQHIKRVSLELGGKAPCIVFDDAEIEEAVEANLR
                     GGFFNQGENCTAVTRLLLHEAIYDKFLTSYIDRVKKIRIGDPTKDETEMGSLVSRAQF
                     ESVKSYFEKGIEEGAKVLHGGRRPKEFPKGYFFEPTVLADVKPSSTVACDEIFGPIVA
                     VIPFKTEEEAIRISNDTIYGLAGGIWTQNIKRALKTAKAIKAGYLWVNTYGGIIPETP
                     YGGFKQSGIGKELGTEGLDMYLETKAVNIYLGDKIPRWYKG"
     gene            14605..>15174
                     /locus_tag="VI935_07665"
     CDS             14605..>15174
                     /locus_tag="VI935_07665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLE25512.1"
                     /translation="MEGKINTLETVREKLLDREEPISFDEAIQLTEIPKEHFPDLISL
                     ARKVTLKYKGDGVFLRSIISAQTGNCPEDCAFCSQSSYYDTEINAHPLMAAERILKAA
                     KQSEKLGAMDFCIVVSAKGPTPKIFKMVLEIVDLLIKETNLKIGCSLGELTEEQAFAL
                     KEHGVWRYNHNLETCRSYFPHVCTTHTYDD"
BASE COUNT         4349 a         2984 c         3114 g         4727 t
ORIGIN      
        1 atagtttaca atctgttcgt tatcaggatc gtgttttagg gcttccttta tcattttgag
       61 agattcgtca gtccttccca tcttttcaaa aagatatgct ctttgaataa gagcgtcgtt
      121 atacaaagcg gaattcttag ggatctgatc gaggatttta agcgactcgt cataccttcc
      181 agtctccgtg taacaaaggg ctagataata tttagccctt tcatcccccg ggtttccaag
      241 tagaatgagt tgaaattctt caatagcttt ttcgtattct tccttctcaa gataaagaag
      301 accaagtcta agctttaagt ctgggttttg atagtcaagt tgttgagctt ttttaaactg
      361 gattgcggct tccgacaggc gattaattct aaagaggaaa ttcccgtagc gtacataagc
      421 atcaaaactg gaaggaaaaa tggttacaag ttctttataa atgttttctg attcatctag
      481 ctttccttgc ttctcataga ttatcgcaag atccaaaagg gctgatgggt aaacagggtt
      541 tagcctcaag gcctttttat aataattttc agattcgaca agatccccct tttcaaggtt
      601 tggccttcca agaaagtaat atccgcgctc gtcgtctggt ataagctcga tgaccctctt
      661 aaaacttatt ccggctttat cgtattcctt tgactcggtt tcaataatgc caagaaatag
      721 atgtgcctca gaatcatttg ggtctagttc tatagccatt tttaactctt taacgcctct
      781 ttttttcttc tctgggtcct ctgaagatgc atagatcttc cctaggagtg tgtggctagg
      841 agcgaagctt ggatttactc gaacagattc ctctagctta ttcaaggcct ctgtaaatcg
      901 gtttacgctc atatatataa cggcaatatt atattttatg taggctgaat ttgggtcata
      961 tttctcggct gtttcaatct ctcgaatcgc ctcttcaaag tttccacgaa aaaagtttag
     1021 tcttgagagt gtgaaatggt aataagcctc gtacttttct gactggggtt cagttttttt
     1081 acttgtgtct gcttttactt gattttctat agatttatga gcacaggaat acggttggat
     1141 cagtaaaaag atgacaagaa aaagtttgag ttgattcttt agttcttgta aattcatggt
     1201 ttttttaact agcatccaag atttcaattt tgattttacc aacaagccta atgatttcag
     1261 agcctctatt gaggtcgcct tctacaattt ttatgtgtga cgcatcagca gggagttgcc
     1321 cgaatgttgg atctactgga atccaactcc ctaaaaagac ctcgttccat gcgtgataat
     1381 agaacttgcc gttgacataa acaaccccaa aaacaatctt tgtgggaatc cctgctgcgc
     1441 gagaaagggc ggcaaagagt gcggcgtgct cgttgcagtc tcctctcctg gtctgaagca
     1501 cgtctagggc gtttggaaga cttacggttg gcgctttttc tagtttttta taaacccagt
     1561 tgttaatctc ctttgctgct cgaagggaat ctttttcgcc tcttaaaatt tctactgatt
     1621 cctctattat ttttggatca tcgctctgga ttaagcttgt aggtttaata taggatttgt
     1681 acgcttcctc agtatagggg atttcataag gaattatttg agaaagatcg cttcgcctta
     1741 cctcgatgat accgtctttg aaaaggtttc tgttatcttc aagattgggt tctttgggtg
     1801 actcaattcc gtcaatcttt atgcggagaa attttagagt tctagggttg tctaaagaaa
     1861 cattcgagga gatagcggtt ttctgaacaa tgtctaaact ctgtagcgtt tcacctaaaa
     1921 ccttttcttt agattctctt aatgccagaa gtcctggctg agaaaactct tttatgatct
     1981 caccctcttt ggttatccaa tatgtagact cagaacccat aaagagcatc ttaactcggt
     2041 acgttttgaa gctaccataa ggggttttaa tttgttcttc accttcaata cttagggttg
     2101 cgagtaaact cttggcatct gcacctgtta gaatcagcgt tggatcaaaa agggttactt
     2161 gataactttt tcctactctg ggcttttgct ctgcaagcca cttaaatata attggagaaa
     2221 ctaaaggttc gttttcaatt gggaaagtga tattggactt gcctgaaact gaggagattt
     2281 gtatatcaag cttttttcct tcttttttac cttcagcgct aaattgaacg ggaccgactt
     2341 tcattgaaaa ttcaaatgcc tggagttcgt atttattcag aaggtaagtc gatgttatat
     2401 ctacgacctg tttagtgcct agtgtatcca tattaagctt ggtttcttca aatatttgta
     2461 ggttttctct ttttggttta taaatagtgt gagaatagcc aatcttatcc cctccggaat
     2521 agatccccat ccattcctct tcataattga cttttaaggc aatagattga gaagcgttca
     2581 ggagaaatat tataagaaag cctaaaaaat acatactagt ttcaccttaa taatctacac
     2641 tcgtttcttg ttgctctcaa gtgtcgagtc gggggtggag acctaatgat cttttaacat
     2701 ccaattcggt aagaaacaca gggtaggcaa cgagtcttgc tcgttgccta ccctgttgga
     2761 ttgctggcta atacatttga tttttagaag tccagtggct ctgccacggt gactttcact
     2821 aaaatcaatc aaggattcct ggttacagga atgctcataa gttggttatg aagaagaagg
     2881 aaaaaggggc acccgttttt aaggctattg ttgcttgcga gctaagcgaa gcaatctcat
     2941 catttgggat tggttcgtcg ttttacttct cgcaatgaca gaaaaaacta ttagagaaaa
     3001 aggtgacgaa gggctggttc ccgacaaagt catcggggat aaagtccagc ctatccccat
     3061 atggataaca aacaaaggga tagtcggggt tttctaaccc cgctatacaa tcccaaaaaa
     3121 ggggactgtt ccccgttttt cttaggcaag ctagagtaat gtaaaaatgg gatagtcaag
     3181 cttcgaccag ctcgatctta agtcgagcgt gcaaggcctg cgcaatcctc tgcaatgtct
     3241 tcaactccgc atttcctccg ttctcaatcc gcgagatcac cgctgggctg gtgtgaagct
     3301 gtgcagccaa ttgtgtctgt gtaagtcctc gcttctctct gagttccgcc atgctaaccg
     3361 ccagccgcaa cacatctaac ccttcctcga aggattctct cactttgggc cgcgctaaaa
     3421 cccgcttctt aaacttgatg tatctattct ttccctttat catgatgctc ctcccatttc
     3481 atactttata tgatatacct ttaaagataa attagcaagc ggtaaccctc tattaatata
     3541 gagttgtaaa ggttaaatcg taaagattga cacccaatgt ctaataaggt tgaataaatt
     3601 ggaaatgata gcgagcgaaa tcgttaacaa aattgataga aaggggcgta taaatccaga
     3661 agagggtcta atgctacttc gggaagccga tttgcttgaa ctcggatctt tagcagactc
     3721 tgtcagaagg agattccatc ccgatggaat cgttacattt gttgcggata caaacccaaa
     3781 ttatacaaac gtctgcgata cggagtgtct tttctgcgca ttctggagac cagcaggggc
     3841 ttcagacgct tacaccttaa caattgatga ggttctaagg attatgaaaa cctcttttca
     3901 taacggagcg accactgttc tccttcaagg cgggcataat ccagaaatta agcttgacta
     3961 ttatatcgag cttgtgagac gggtaataga ggaggttcct ctgcttcatc ttcatgcatt
     4021 ttctgcgcct gagattagcg ctatagcaaa ttattctcac atttcgacca aagaagtatt
     4081 ggaaaaactt tgggatgcag gactaagaac catccccggt ggaggcgctg agattctaag
     4141 taataaggtg agaaaaaaga taagccccct taagattgac gccgacaagt ggatacaggt
     4201 tacaaaagaa gcccacgaga ttgggtttaa atccacagcc acaatgatgt tcggccatgt
     4261 tgaagaggat gtggatataa tagagcacct tgaaaggatc agaagccttc aggatgaaac
     4321 aggcggtttt ctggctttta tcccctggac gttcaagcct aggaatacct tccttgaaaa
     4381 aaagatacct cgggagatta gcggagatag ataccttcgt gtcctttctc tatcaaggat
     4441 atatcttgat aattttaatc aggttcaagg ctcatggttt actcagggga aaaagatcgg
     4501 ctctatttca cttcactatg gtgcaagtga tttaggtggg accctatacg atgaaaacgt
     4561 tctcggatgc gcagagaaca agcttagagc aacgatgact gagctcgttc atatgataaa
     4621 aagcgcgggt tttaagccag cacagagaga tacattttat aaggttatta gatacttcta
     4681 attttcgtga ttcgttgatg gtggttcgtg gatcgcgatt catagtgcga gtcattatac
     4741 atgaaacccg atcctcgaat cacgaaccac gcactgttct tgactttctc cctatttatg
     4801 aaagtatttt cttcaatgat ttgattggag gataaaacat gttaaaaaag gtttatgttc
     4861 catctttgtt ttgtactttt atcttgattg gaataattct aatactttca tgtggggcgc
     4921 agagaactta tcagactaaa tcccctcccg gaggatttgc tagttatgtt attgttgaga
     4981 tccctgattt taagacatct gtaaatcctg tgcctccgga cgttatttgg aaaataccta
     5041 atgagattgc ggaaaaatta agaaaggaac tactttttac aggggtaagc cgctcacctg
     5101 tagacttatc tgaccgtgtt cttatattgg atggtactat ggtaggttat acccctacaa
     5161 tttggtataa gcgaatagtt aatacaggga gagtgattgc taacgtgcgc tttattgata
     5221 aatctgagaa caaggtaata gctgagtcat cctttgaggg aacggttgaa ggaggttatt
     5281 ttggcggagg catgcacttt gcgaatgtcc gtttagctga tgaggttgtc caatatataa
     5341 aaagaaattt ttctccacaa tgaaatatca tgggtattcg gtttttaaaa tctttacctt
     5401 ccatgcatca ccagtaaagt ctacaaggca ttcgattgaa ctaaaaaaca taaaaacaaa
     5461 gtcaaagaac tcataagcag agcttgaaaa cacaggttta tatagaaaat cagaaatctc
     5521 catgctgtca aaattaatac actcacatgg agatagctcg aaatcaatcc catcttcttc
     5581 catattttct tctgatttta gtaagtttac cgatacatat ccctcaattc cctctttttg
     5641 aatagaaagc ttaataggtt tttcttgaca ctcaagtaga gccgatgcaa gaaaatcatc
     5701 gaggttgctt atgtgaattt ttttctccac taagctttct atgtccatgt gacaaatctc
     5761 ccatccctga gtgaaactcc ctgcggcaga gccacggggc aactaaaaat caaaagtatt
     5821 agcctacatt ccatcctccc aaagtagggg ctgatgcctt tatcagccct aaaagctcgt
     5881 tcggggaacg agccctacac cctgtgcaaa taaaattgta gggactgatt cccaatcagt
     5941 gcaaatatca ttgcccgttc cggggaacgg gcactacatt ctctgaagta atttcccggt
     6001 tcattaaata tgtagcggcg acctttaggt ccccataaaa taataatgtg gaggactaaa
     6061 gtcctccgct actgtcatac ccgaaagttc cccatttcat acaaatgggg acaaggttat
     6121 cgggcatcca tgacatacta ctttatccga gattagaaaa cctcggctat cccccgtgga
     6181 ggtctgaaga ctccgctaca ttgctgctaa catcaaatct aaagcatttt ttaaggttat
     6241 ttgcatctct caaataaata acaacttaaa ttgctttgcc cgctcttaaa atctttactt
     6301 tttgaaagac aatagttttt cttgagagtt gattcagaaa gcttctctgg gtcagtgaac
     6361 cattcatggc tgtatattaa ccataccctt ttgtgatttt gtattaaatc ttctaatgca
     6421 ggtacgtcct ctatggtcat ctttctttca ctgaaatttg taggaagacc atgtttttca
     6481 agcattagat tgtatctatc aaaataatat tcaaatggaa tttgcccaag cgctgagcta
     6541 aaaagaacca aatcttcttt acctgaattt tgtgaaacat actctgctgc taaatcccat
     6601 cgctcttttt taaattctgc ataatatttg taaagtgagg ctgagttaag aacactaatt
     6661 aaaactatcg tcaaagcaaa aacccatttc ctcttgatcg aagagattcc taatgctatt
     6721 aaaacgatat acggccctga tgcccatata agggtttgtg ttgctaggat tgaatgcttt
     6781 aaacttatta atagttgggt ccctatagga acaaaagaga tgagtaagag tagtactaaa
     6841 gatcgaggat tgcgacgtcc ataaataata cctgctagga aaagaaggct aaagaatatt
     6901 atggttacta aaaccagtct tccatgaatc aagctaaaat taccgacatc tatccagtta
     6961 aaaagccagt atgtaggccc attgttaaag gtggcaaaaa gattaaatat gctctcaagt
     7021 gtaagctgag gtgtatagaa actctttcct actgaagagc tctgttcaaa gaaaaaagag
     7081 gtccagggag cccagagggt tattatgatt aataaagaaa gggtccagaa tttgaaaaat
     7141 ggtctatgac gattccagaa tattatgaaa aagagatttt gagccagtgg tagaagtaaa
     7201 gcaacgttat ggcagtaaag ggcagctact gttgaaataa cataaccaat ggagtaaaag
     7261 atagtttttt ttgttagttt aatctcttca cctatacaaa gcatcttggc catgaaaaag
     7321 attgagagaa ggacaaagaa tgtcagaatg ctatacatcc ttgcttcctg ggagtaatag
     7381 acgtgaaatg gagataacgc aagcattagg gctgaaatta gagcaggaag gttaccaaat
     7441 aggtctttca caattaggta aagtaaaggg atactaagaa tcccaaatat cgctgataaa
     7501 agccttactt caaattcccc atttccaaat acatttatcc ataggctaag gacaatataa
     7561 taaaatggtg gatgaacatc tgcgctcgtt tgttttatta ttatgtttcc cagagattca
     7621 ttagcaaacc agacgctaat ggcttcatcg agccacaaac tatttgatcc cagttgataa
     7681 agacggagga atattcctat taagatgatg agtgggagta atatgaatga gagacgattg
     7741 ttaggtgtga ctttaagcat tctggataga gctttgtagt ttgttagtag gttagtttgg
     7801 taaacctaat ttaccaaatt agatagtgat taacaacgtt taaaataggg aggtggtgca
     7861 attaacacag cttttgctat ttttataaac ttacagcacg atataagtgg tcaatttgat
     7921 tcatttcgtt tgcttctttt tgacattttt ggtgatttga gttctacgtt attctgttct
     7981 cgataacggg ccttcggttg tctatcttgg ttttgaaatt gattaatatt atcctcctgg
     8041 gacgtaatcc atcttttatt cgagttaatc ggatagtctt tctgctttgg cttatcagcg
     8101 ttattttggt atggaaatct cttgctattg ttgtttgccc tttttacatg taagtagttt
     8161 ggttcatacc ctttccgatt ttcggtgtat gcactatggg ttctttttaa cgaattcctt
     8221 tttttattct ccgagtgcgc attatattgt tgatcaggcg cgtccttaat attctcttgt
     8281 ttttgatatc tttcttcatt tttaattttt accttattgt ttgagtagct atggtcatta
     8341 tcgtccctga ttgttaggga gttctttaaa gactcttttt taactttagg gtagttacga
     8401 ttgggtaagt tttgaagctt attataactc gagaatctgt tatgggtgtt ttcgatttgc
     8461 agtgctttct ttggggcttc agcatttggt agagctgctc ctttgttttt tttgactaaa
     8521 tcaggcctga atatattgta attatcacca tctaatttgt ttacattgac gccggtttta
     8581 acaattcgat cagtatctat atctttttca actagattta ccttttcaac ctttgttctt
     8641 gaaaccctct caacaaaacc gacatcagga ccacggttta cgacattatt atttacgatt
     8701 cttgcattat ttatatgcgt cgcttctttg aaaattttaa cgttatgtga ttggggaaca
     8761 actgctgaat gaacatgatg atgtaaaaat ttgtgcgagg gtacaaaaac acagtggctt
     8821 gggggtgtat agtaattgta attattgtaa taattataat tagtaaaccc aatatttatt
     8881 cccaaaaaga agttatcatc aggtggaaga ggagcccacc caatgtagcc gggactggta
     8941 taccatgtaa cccatgcggg tgcccaaata ttgcccggta tccataccca tccgtagtaa
     9001 tcaagaaata tccatcttcc atagtggtat ggcgcccaac cccaaggctc ataagaaacc
     9061 catgtccatc catagtctga ccacacccag cgcccatcag tatatggcct ccagtggggt
     9121 ccaacgcctg tgggatacca tacgtttcca tatcttggca cggataccca attaccgtag
     9181 ggcgaaagtg tgttgaaaaa aagattgaac ccaactgcta cgttggcttg agcagtgtta
     9241 ttattttcaa cactgaggat tattgctaag attaagaagg ttgctgccca tttctttttc
     9301 attatattaa tcactccttt tgataattcc catatatgat taaactcata atttctatca
     9361 gaggtttcaa tctttgctga cgtgaaggat tattgttggg acattactta actcgacaat
     9421 tccctacagc ttactacttt ttttcaactg gcatcccgaa cacattcaca gagcacacgg
     9481 gataggcggg actttctagt cccgcaatgg catgcggggt tcgaaaaacc cacctatcga
     9541 tatactatca tttgctagca cctcgaataa ctcggctaag ctctataaaa caaaaacaag
     9601 aatgagattc ctcgctccta tcagtcgctc ggaatgacag aaagaggagc cgtcattgca
     9661 atcacaaaag gaatgtcttt atatgccgat accctgtagc ttggaggctg tccgataaat
     9721 gtaaaaaacg gcattcctgg gggtagcaaa acccctccca ggttgccgat cattgatcct
     9781 ggggcattct gaggggtcaa atttttacca tccataattg tcggacagcc tccttgtcga
     9841 agggttctct atttacaaaa tcctaagttt gtaatagtta tgttaaaatt cctagggaac
     9901 aaacttattt gagattggtt gcccaactac ttgggtcaaa cctggagcta acttatgata
     9961 aacgctggag taggatattc tcttaatgaa aacggtgagc aggctgcaaa agaagcagct
    10021 aggcaagcta ttgagaatgg ggatataacc aaagccgact tcgcagtagt tttttgttcg
    10081 tttcctcaca gaggaagtta taaagatata ttaaaatctg tatgccagat cacagggact
    10141 acaaatatta ctggctgcag tggtatagga gtccttacta attatggaga agtagaggca
    10201 aaacctggtg ttgcagttct tgctgtttca tcagatggaa tgcatgcgac ctcctttctt
    10261 gtacaccaca cagatgatgg cggcatgaaa gccggtttgg aaattggagg gcgtttgatt
    10321 cctataaaag atgaaaacgc acttcttgct attcttccag acccatttca tattcatccc
    10381 gagcttctca tgagaggaat tgaatctcgt attggagaaa tacaaatagt tggcgcatcg
    10441 gcttctgaac acccggcttt tcatgaaaca tatgagtttt acggagaatc agttgtttca
    10501 ggagctatat caggatttat gattcaaggc tctttcaccc atagaatagg aattactcaa
    10561 gggtgccagc ctgtagggat tccttgcatt atcacaaaat ctgaccagaa tcttatcttc
    10621 gagctcgatg gccagcctgc ttttgaggtt ttgaagaagc aggttcctaa aaggatactg
    10681 gagaacccaa gaaaattatt gcgtcttttg ttcgtgggtt ttagtcccga tcccaaggag
    10741 acagagataa ttggcggaga gtatcttgta cgaaacctaa tagggataaa cccaaatacc
    10801 ggcgttatag gagttgctga gaatgtcagg gaagggcaga ttatgacgtt tactgtacgt
    10861 catcccataa tggcgcgaga agacctaaaa caaatgcttg agcgattagc atcgtcgagg
    10921 aatactcaaa agcgatttaa atttggtttt tactttaatt gctgtgcaag aggttcgtca
    10981 ctttatggtt atgaaggaat tgacacagcc tatatcaccc atgcactagg tgaaattccg
    11041 attattgggt tttttggtaa ctcagaaatc gcaccgctaa aaggaatcaa ccgtctcttt
    11101 acatatacag gagtgctcgt gctgatttca gagtaatgat ttatccaatc ttaacgaact
    11161 taggaattac atcagcgtac tcttaacatt taggagcgcc atcttggcgc gataaattgc
    11221 cactcatata caaatctgcg actggaaagt cgcagctatc gaaggttaat tttcacagaa
    11281 tcacaccgcg atagctgagg ttttcatgtc tcgtcttagc gcgataaatt gccactcata
    11341 tacaaatctg cgactggaaa gtcgcagcta tcgaaggtta attttcacag tattacacca
    11401 cgatagccga ggttttctaa cctcggataa acgtagttat ggtattgatg cacaattaat
    11461 tcttgtcccc atatgtatga aatgggtaac attcgggcgg gcatgagagt gcgattaccc
    11521 atatacgtag gtgcggcatc ccttcgaccc ttcgacaaag ctcagggctc aggacaggcc
    11581 ttgccgcgat aatcacgaaa caccgatttt gctgaggctg gaagccgctc ctacttagtt
    11641 ctactggtga attattacat atattgctaa ggttaatata ggatggttta ttaaaaataa
    11701 agggaacttc tgggttgaaa gttatcaagt tacgtttatt atttaaaaaa atttttccaa
    11761 aggagatatt gatgggttac aaattctttg aactctcaga agaggattca gtaggtgttg
    11821 tccgtttgaa tcgcccacca gttaatgcct ttaatctcga tatggctaga gaatttgatg
    11881 ctcttttaga tgacgtagca aagattgaag gtataaagtc cctagttgtt acaggaaata
    11941 aaaagatatt ttctgcgggc gccgatattg gattgattag aagcagtgag tggagttatc
    12001 ttaaggagtt ggttgaaatt ggacagaggg cgtttcaaaa aatggagaat atgactaaga
    12061 tagtgattgc tgtgattgaa ggtcattgca tgggaggtgc tatggagttt gcccttgcat
    12121 gtgacagaag gtttatggca ataggcgaag caaaaatcgg gcttccagag gtaaagcttg
    12181 gactaatgcc agcctgggga acaacctata gacttccatg tcttatagga aaatctaagg
    12241 ctcttgactt aatggtgaga gggacccttt taaatgctga ggaggcgaaa gcgattagac
    12301 ttgttgacga gatataccct caagtagaag taagcacaaa agcaatggat tatgcaaaat
    12361 caattgctat tggagcaacc ttcgctatcg gtaagattaa aagctgtatt aataaaagtt
    12421 tcgatttaac ttttaaagaa agcatggaac tagagtgcaa ttctcaggga gaattattta
    12481 aaacccaaga ttacggagag ggtacgcaag cgtttttaga aaagagaagt ccaaggtttg
    12541 taggtaagta agacattacg gtagagacga cccgtcgggt cgtctctaca taaattcgcc
    12601 agccctttta gaagattgat gcttctaccc cttataccac cttgggattt tatcgccgag
    12661 atatatattt acagcctttg tctcaagata catatcaagt ccctcagttc cgagttcctt
    12721 tccgatacca ctttgcttaa acccaccgta aggcgtttca gggatgattc cgccgtatgt
    12781 attcacccat aagtaccctg cttttatagc ctttgccgtc ttgagggcgc gttttatatt
    12841 ctgggtccaa attcctccag cgagtccata gattgtgtca tttgaaattc gtattgcttc
    12901 ttcctcggtt ttaaatggaa tcacagcaac aatgggaccg aatatttcat cacatgcgac
    12961 agttgacgaa ggcttcacat cggctaaaac agttggctca aaaaagtagc ctttgggaaa
    13021 ctcttttggg cgtctgcccc cgtgcagaac cttagctcct tcttcgattc ccttctcaaa
    13081 atatgatttt acagactcaa actgtgcacg ggagacaagt gagcccatct cagtttcatc
    13141 tttagtaggg tcccctatac gaatcttttt caccctatct atataagatg tgaggaattt
    13201 atcgtagata gcttcatgca gtaggagacg ggttaccgcc gtacagtttt ctccctgatt
    13261 aaagaagcca cctcttagat ttgcttcaac cgcttcttct atttcagcat cgtcaaaaac
    13321 tatacatgga gccttaccgc ctaattcaag cgaaaccctt tttatatgtt gtgaagctgt
    13381 attcatcact ccctttccaa cctctgtaga gcctgtaaat gcaatcttac ttatatctgg
    13441 gtgttctatt aaagcctggc ctgctaaact tccctttcct ggaattacat tgaatacacc
    13501 ttcaggcaat cctgcctcgg ctgttattcg tgcaaactcg aggatgccac atgatgtatc
    13561 tgtcgcaggc tttaatacaa ttgtgcaccc tgcagcaaga gcagctccga ttttccagaa
    13621 tgatagaagg agtggaaaat tccaaggaat aatatgcgcg gcaacaccta caggttcttt
    13681 taaagtaatt gtgagctggt catctgatac tgaaatgctt tcaccattta gtttggtggc
    13741 ggctcctgcg tagtattcta gcgttcgtat tgagtttcct aactcaacca gccggctttc
    13801 acgaatcggc tttccagttt ctagggtgtt tgtcatggct atatgctctt tgtgcttttc
    13861 aagaagttct gcaagacgga atatgaattt agcgcgctcc cttgacggga cgtgagacca
    13921 tttttcaaat gcacgcttgg cagcctttac agccctatct acgtcctctc tttgagcctc
    13981 ggcaatctct gcaaagggct cctctgtagc cgggttctct cttatatagg ttttcccact
    14041 aactgaattg atttcttttc cgtcaatgaa gagaccatat tttcttatag gttttgccat
    14101 aactattctc tcccttttgt gtgatatgaa ttctctaact tgaaactccc tttagcttgc
    14161 tacatggttt acaccgtcat tgcaaacact tttgtggagt ctgcactgag aacggatagg
    14221 cgggacattc ttgtcccgca acaggatgcg gggtttgaaa accccgccta tcgataaact
    14281 ccatgaagca atctgacaca aagtgtcatt ccgaacgaag tgaggaatct aattgggatt
    14341 gcttcgtcac ttcgttcctc gcaatgacac ttccgtgctg tctttgcaat gaccaaagta
    14401 actacctttt ccatgctgat accctagctt gctgcaggat tcttcattat agatattaca
    14461 actgtataaa ttacaagtct agcttatggg tatatattct actaactgaa ttttttagat
    14521 tatttagttc aaatgttata acttacatta tcagtcttat tgacaacgat tgtgctatag
    14581 gttaaaaagt tttactggct aattatggag ggcaagatta atactttaga gacagtaaga
    14641 gaaaagttgc tagataggga agagcctatc agttttgacg aagccataca attaaccgaa
    14701 atccctaaag aacattttcc tgatcttatc tcactcgcta gaaaggtaac gctgaaatat
    14761 aaaggagatg gcgttttttt acgttctatt ataagtgctc agactggtaa ctgtccggag
    14821 gactgtgcat tctgttctca gtcatcctat tatgacactg aaataaatgc tcaccctttg
    14881 atggctgctg aaagaatatt gaaggctgca aaacaatctg aaaaattagg cgcaatggat
    14941 ttttgcatag ttgttagcgc taagggaccg acgcctaaaa tttttaaaat ggttcttgag
    15001 attgtggatc ttctaataaa agaaacaaat ctaaaaattg gatgctctct aggggaactc
    15061 acagaagaac aggcttttgc actcaaagaa catggagttt ggcgttacaa tcataatcta
    15121 gagacctgcc gcagttattt ccctcacgtt tgcactacac atacatatga tgat
//
LOCUS       DATDRL010000224         6427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_418508, whole genome shotgun sequence.
ACCESSION   DATDRL010000224 DATDRL010000000
VERSION     DATDRL010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418508"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..562
                     /locus_tag="VI935_07670"
     CDS             53..562
                     /locus_tag="VI935_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25513.1"
                     /translation="MIQKLRTDLVLKLSPIKLLIINADGLLSENQTPYDFQNQSLSNG
                     QGVKALKKLNVESIAISSKKSEALSSILNRLGIELLYQSVFQKEYLYTKLKFEHSVSD
                     YEIAYIGGELSDLSIINQVSFPVAPADAALEVKTKSYYVTYGIGENAVREVVDLILKA
                     KNHSNGFRK"
     gene            741..1874
                     /gene="queG"
                     /locus_tag="VI935_07675"
     CDS             741..1874
                     /gene="queG"
                     /locus_tag="VI935_07675"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HLE25514.1"
                     /translation="MSIAAVSKSIKEKALEIGFDLIGISPVDSFPENQFYKEWLARGF
                     AGEMKYMEKEPEKRENIRKILPDARSVVSCGLNYNTNYPYSMNETDQEKGWISRYAWG
                     DDYHDIMKKKLQILLESIKANSPQEIKARVYVDTGPVLERVYGKYSGIGWFGKNTCLI
                     NQKVGSWIFVGEIITNIELDYDNTAPDRCGTCTRCIDACPTGALLEPYVLDSRRCISY
                     LTIEYRGMIPLEHRDKTGNNIFGCDICQDVCPWNRRAKVTDESSFKPRDGLYNPDLAS
                     LSQLTEKDFRRAFKGNPIKRAKGNGLLRNIVVAMGNSGLKDFIPYLEESLKDKDPIVR
                     AHSAWALSKLEGEDSYNTLSNHFEVESDSIVREEIISILNMNG"
     gene            2043..2465
                     /locus_tag="VI935_07680"
     CDS             2043..2465
                     /locus_tag="VI935_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLE25515.1"
                     /translation="MSEDFKEVLNVNEMFYKALGGGNLGLMEEVWVKDPRAKCVHPGW
                     PILLGWEAIRQSWKNIFDSGGPTKIQISNVNVEMSGDLAWITCIEHICHIIRDQVQIS
                     MAQATNIFERNGTHWLMIHHHASPMPMPRGKAADDKLQ"
     gene            2485..2700
                     /locus_tag="VI935_07685"
     CDS             2485..2700
                     /locus_tag="VI935_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25516.1"
                     /translation="MCCEDLEQALRDEIIIIMDKSYLEDGRIMNMVESPFYFRSERGN
                     SGYDYYGINCCPFCGMAISIVAQGFSV"
     gene            3108..3425
                     /locus_tag="VI935_07690"
     CDS             3108..3425
                     /locus_tag="VI935_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25517.1"
                     /translation="MAIKLLELRKFAIEKRVDVGFKDKNSGELCVVDKHGIAKIPGKN
                     TFKEPFRNSIEEALKTATEFIIDDRKGRQVVLVDNMAEMIADVLKPKTQAVHGAAAKK
                     EED"
     gene            complement(3550..5067)
                     /locus_tag="VI935_07695"
     CDS             complement(3550..5067)
                     /locus_tag="VI935_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HLE25518.1"
                     /translation="MNSINVPSFTGLNSSPKSSHRVKGKLLSFLFIPTDIASLVYFRI
                     AFGGIMLWEVIHYCQYDVIKRYYLDPKFLFTYYGFDWVHPWPGDGMYYHFIALGVLSL
                     FIILGLFYRVSALLFFLGFTYVFLLDQTHYLNHFYFVSIISFLMIFIPANRSFSIDAR
                     LHPTIRSDTAPAWALWVLRAQMGIVYFYGGLAKLNGDWLHALPFQLTLPAISNVPLIS
                     SFSAWEWIAYFVSYTGLLFDLFVVPLLLWRKTRLFAFCWALAFHITNTFLFSIGIFPL
                     FSIAATALFFPPDWPRRFIRRFWGSVSKEKRAKKTHPHPVSSVTASLQRGQRVTMVLL
                     GIFFAFQLLLPLRHHLYPGEVSWTEEGHYLSCRMMLRRKIAAARFFATDPVNNKSWEI
                     NVRDYLNRWQSQEMIKRPDMILIFSHHLAKELRKQGYEQIEIRAKVFVSLNARKPHYM
                     VDPTVDLGSQPRSIIPAKWIMPLVEPRPTREQIKDFLEKLINLMNSKHRQKEQTQ"
     gene            5417..5683
                     /locus_tag="VI935_07700"
     CDS             5417..5683
                     /locus_tag="VI935_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25519.1"
                     /translation="MDEIRSIFSDKWIRRFSVLFLVLGLSFFLATACNDDDCFFGQID
                     FAAVENEAECEQFCFVNLCDDCEFVAPNECTGIDCAVCEIIEDI"
     gene            6221..>6427
                     /locus_tag="VI935_07705"
     CDS             6221..>6427
                     /locus_tag="VI935_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HLE25520.1"
                     /translation="MYQYFLLENKDKYHGFLYYKQAPNFTLTDHNGKKVTLSEFRGKL
                     ILINWGYTRCPDICFTTLSVLRDVM"
BASE COUNT         1987 a         1079 c         1431 g         1930 t
ORIGIN      
        1 attttagagc ttaaaatgat taaccagttt agactttgat ggaggagaaa ggatgatcca
       61 gaaattaagg actgacctag ttctaaaact tagtccaatt aagcttttaa taataaatgc
      121 tgatggactt ctttctgaga accaaactcc ctacgatttt caaaatcaat cccttagtaa
      181 tggtcaagga gtaaaggctt taaagaaact taatgttgag agcatagcta tttcttcgaa
      241 aaagtctgaa gcgctttctt ctatactgaa ccgtttgggc atagaattat tgtatcagag
      301 tgtttttcaa aaagagtatc tttatactaa actcaagttt gaacattctg tttcagatta
      361 tgagatagct tatatcggtg gtgagttatc tgatctttct attattaatc aggttagctt
      421 tcctgttgct cctgcagatg ccgcccttga ggtcaagaca aaatcatatt atgttaccta
      481 tgggattggt gaaaacgccg taagagaggt ggttgacctt atcttaaaag ctaagaacca
      541 ttcaaatgga tttcgcaaat gaatgatacg ggaaggtttg atctttacga cattggcttg
      601 ttcaggcaat ttttaggtgt cgtatcatta gttgagtggg gtacagcttt tagccttgtt
      661 aaattcaagc ttatatcata acttcaactt gcaataaaat cgttagagat agcgcggtcg
      721 ctatgataca ataatcttta atgtctattg cagcagtctc caaatctatt aaagagaaag
      781 cattggagat aggctttgat ttaatcggga tatctcctgt tgattcattc cctgagaatc
      841 agttctataa agagtggctg gcaaggggat ttgcgggtga gatgaaatat atggagaagg
      901 aaccggaaaa aagagagaat ataaggaaga tacttcctga tgcaaggtca gttgtttcat
      961 gtggtttgaa ttataatact aactatccat actcgatgaa tgaaactgat caagaaaagg
     1021 gatggatttc gcgatatgca tggggtgatg actaccatga tattatgaag aagaaattac
     1081 aaattttgct agagtctatt aaagcaaaca gtccccaaga aattaaagcc cgtgtctatg
     1141 tggatactgg tcctgtttta gagagggttt atggaaagta ttcaggcata ggatggtttg
     1201 ggaaaaatac atgcctaata aatcagaaag tcggctcctg gatctttgtt ggagagatca
     1261 taacaaacat tgagcttgat tatgataata ctgctccaga tagatgcggc acgtgcacac
     1321 gctgcattga tgcctgtcct acaggggcgc ttcttgagcc atacgttctt gattcaagac
     1381 ggtgcatttc gtatcttacg atagagtata gaggaatgat cccattggaa cacagggata
     1441 aaacaggaaa taacatcttt ggttgtgata tatgtcaaga tgtctgtcca tggaatagaa
     1501 gagcaaaagt gactgatgaa tcttcgttta agccaaggga tggtttgtat aaccctgatc
     1561 tagcctccct ctcacaatta actgaaaagg attttagaag ggcattcaaa gggaatccga
     1621 taaagagggc gaagggaaat gggcttctta gaaatattgt agttgccatg ggaaattcgg
     1681 gcttgaaaga ttttattcct tatttagagg aatctcttaa ggataaagac ccgattgtaa
     1741 gagctcattc tgcctgggct ttatcaaaac tcgaaggtga ggattcctac aataccttgt
     1801 caaaccattt cgaagttgaa tcagactcta ttgttaggga ggaaataatc tctattttga
     1861 atatgaatgg ataaaatgga actgcaaatc ccaaattgca gattccaaaa cccaaacaaa
     1921 tcaaaatccg aaattctaat gaccaaaacg tttggaattt gtattgtcgt tagtaaaatt
     1981 tgtttgtgat ttgctgcttg tgatttggtc attgagctgt tttattttct gggggcttac
     2041 ttatgtccga ggattttaaa gaggtactca atgttaatga aatgttctac aaggcacttg
     2101 gaggcgggaa tttgggttta atggaggagg tttgggtaaa agacccaagg gcaaaatgcg
     2161 ttcatccagg ttggccgatt ctcttaggat gggaagcaat tcgacagagt tggaagaata
     2221 tctttgattc tgggggtcct acaaaaattc aaatatcaaa tgtaaatgta gaaatgtcgg
     2281 gagacttagc gtggatcact tgcattgaac atatatgtca tataattagg gaccaagttc
     2341 agataagcat ggcccaagcc actaacatct ttgaacgtaa cggcacacat tggctaatga
     2401 ttcaccatca tgcttcacct atgccaatgc cgaggggaaa ggcagcagat gacaagttac
     2461 aataaaaaat aaaggacaac attaatgtgt tgtgaagacc tggaacaggc tttgcgagac
     2521 gaaatcatta ttatcatgga taaatcgtat ttagaagatg gtaggatcat gaatatggta
     2581 gagtcaccgt tttacttcag gagtgaaaga ggaaatagtg gttatgacta ctatggaatt
     2641 aactgctgtc ctttctgcgg aatggcaata tcaatcgttg ctcagggttt ttctgtttag
     2701 aattaaaact aaaagacaaa tattgcaatt ctattgtttg attttgtata acgaagactg
     2761 tcgttgcgag gaatccttcg acatagctcg gggtaaactc cgtgacaaag gttccgtttt
     2821 gtttgtcatt gcgaacgaag tgaagcaatc ggtttgagat tcctcactgt tccttcgacg
     2881 gagtttaccc cgagtgtagc cgaagggctc aggacatggt tcggaatgac acttcgtgtc
     2941 agattggttc gcggggttta cattgagcac ttcgatttcg tcagcacagg cacttcggca
     3001 agctcagtac aggccccgcg aatgtgatcg caatgacgat tccctgatga atgcagtaat
     3061 gtttcataga attggtatta gacgatcaga cataggagtt aagtgatatg gcgataaagc
     3121 tattagaact aagaaaattt gcaatcgaaa agagggtaga cgtgggcttt aaggataaaa
     3181 attccggtga attgtgtgtg gttgataaac acgggatcgc taaaattccg gggaaaaaca
     3241 catttaaaga acctttccgt aacagtattg aggaagcact taaaacagcg actgagttta
     3301 taattgacga tagaaaggga aggcaggtag ttttagtaga caatatggct gagatgattg
     3361 ctgatgtgct aaagcctaag acacaggcag tgcatggtgc agcagcaaag aaggaagagg
     3421 attaattaag tagataatat caaaataagg ggaactcggc ttattctata aagcagaacc
     3481 tgcgcattgt ttttttacca tcttcaagtt aattaccaga gtttgtaact cggacataaa
     3541 aactcgagat tattgtgttt gctctttttg cctatgctta gagttcatca ggttaatcag
     3601 cttctcaagg aagtccttta tttgttctcg agtcggacgt ggttctacaa ggggcattat
     3661 ccattttgct gggattattg aacgcggctg tgaacctaga tccaccgtcg ggtcaaccat
     3721 ataatggggt ttacgagcgt tgagggaaac aaataccttg gcgcgaatct ctatctgttc
     3781 gtatccctgt tttctcaatt ccttagcaag gtggtgactg aaaatcagga tcatatctgg
     3841 tcgtttaatc atctcttggg attgccaacg attgaggtaa tctcgcacat ttatctccca
     3901 gcttttatta ttcaccggat cggtcgcaaa gaacctcgca gcagctatct tacgtcgaag
     3961 catcatgcga catgagaggt agtgaccttc ctctgtccag ctaacctctc ctggatatag
     4021 atgatggcga agcggtagaa gtagttgaaa agcaaaaaaa atccctagta agaccattgt
     4081 gacacgctgt ccacgttgaa gtgatgctgt aacagaggag acaggatgtg ggtgggtttt
     4141 tttagcccgc ttttctttag aaaccgagcc ccaaaacctt cttataaaac gtctgggcca
     4201 atcaggaggg aagaagagcg ctgttgcagc tatggagaac aaagggaata tgcctatact
     4261 gaacaaaaat gtatttgtta tgtgaaacgc taacgcccaa cagaatgcaa aaagacgtgt
     4321 ttttcgccat agaagaagag gtacaacaaa tagatcgaat agaagaccgg tgtagctaac
     4381 aaaatatgct atccactccc aagcggaaaa ggaactgatt agtggaacat tggagatggc
     4441 gggtagcgtg agttgaaaag ggagggcatg gagccaatca ccgttcagtt ttgctaaccc
     4501 accatagaaa taaactatgc ccatttgcgc gcgtaggacc cagagtgccc atgcaggtgc
     4561 agtatctgat cggatagttg gatgtagcct ggcatcgatt gagaaggaac gatttgcagg
     4621 tataaatatc attagaaaac taattatgct aacgaaatag aaatgattca gataatgtgt
     4681 ctgatctaac aggaatacgt aagtgaaccc aaggaaaaat agtaaagcac taacacggta
     4741 gaatagacct agtattatga agagtgagag tacgccaaga gctatgaagt gataatacat
     4801 gccatcgcca ggccacggat gtacccaatc aaagccatag tatgtaaaaa gaaattttgg
     4861 gtctaaatag tagcgtttaa ttacgtcgta ttgacaataa tgaattacct cccagagcat
     4921 gatcccacca aaagcaatac ggaagtaaac aagtgaagct atatcagtcg gaataaagag
     4981 gaaactcaat aattttcctt taaccctatg cgaggatttg ggtgaggagt ttaatcctgt
     5041 aaaagaaggg acgtttatgc tattcatttc atactttttc cacattatca cttctaacag
     5101 tagattgtca ataaattgaa ttttttatca aatgtagtct taaatcgaaa attccctgta
     5161 actcggaggt tgtccgataa atgcaaaaaa cggcattctt ggggggggta gcaaaacccc
     5221 tcccaggttg ccgatcgttg atcctggggc attctgaggg gtcaaatttt taccatccat
     5281 aattgtcgga cagcctcctt gctgtagggt gtttcattat ctttttaata ttttttctat
     5341 atttttttct cttgacaata gattatcttt ttgggataat tacaagtatt gaatatatac
     5401 ttaaaggagg agagtgatgg atgaaatcag aagcattttt tcagataagt ggatacgccg
     5461 tttctcggtt ctttttttag ttttgggact ttcgtttttc ctggctacgg catgcaatga
     5521 tgacgattgc tttttcggtc aaatcgattt tgccgctgtt gaaaacgaag cagagtgcga
     5581 acaattttgc ttcgttaatc tttgcgatga ttgcgaattt gttgcaccga atgaatgcac
     5641 cgggattgat tgtgccgtat gtgaaattat tgaagatata taataggtga ttagtaactt
     5701 cagtactatg gttgcgcttt tggtagctaa tctagattgg ttagttcgat tccttaggca
     5761 tgactaagct gattttcaat aaatctttta aatatagtgc atttagcatc gttggataaa
     5821 ttatggagga agttagacct ccataatctt tatatgtatt gatatatgat aagagtaagt
     5881 taggggcgaa ctattgttcg ccccttgtgt ttaaaatatg tttctcgctt tataagggtt
     5941 tactagtacc tcctctatcc cgtcacctat tgccaataaa cataccgata taactagttc
     6001 ttatatctta tactgtaact ctcctataat aaattaatgg tctttaacat aatggtctgg
     6061 caactgtgtg tcacgaggta ttgagtggtg aacttctaat tatttttagt atgttatatt
     6121 atcaacaaaa tatcacatca aatcagaaaa aatcgtgaca aaaaggaatg caactataat
     6181 ttctctatta attttgacgt tacttggttg gggattgttt ttgtatcaat actttttgct
     6241 tgagaacaaa gataaatacc atggctttct ttattacaaa caagctccaa attttactct
     6301 tactgatcac aatggtaaga aagttactct ctccgagttt agaggcaagc tgattttaat
     6361 caattgggga tacacaaggt gtccagatat ttgttttaca actctttccg tgcttcggga
     6421 tgttatg
//
LOCUS       DATDRL010000225         4016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3260919, whole genome shotgun sequence.
ACCESSION   DATDRL010000225 DATDRL010000000
VERSION     DATDRL010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3260919"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1903)
                     /locus_tag="VI935_07710"
     CDS             complement(<1..1903)
                     /locus_tag="VI935_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLE25521.1"
                     /translation="MKSKETINVIILAIIVVMIAGIFVLDLFTPRGIPDWGLYLVPLF
                     IMSLITQQRYTFLLTTVCTVLLITGFIYSPPGIKLEIALISRLLGVCLLWFIVVLLVR
                     RKLSEEEVRLLNTITEAVHKSSNLREVFNIALDKVMELTDIDIVGIYLVDEATNEAVL
                     EAHRGYPDKYIERAGRIKYPKGVTWKVINSGETYIVQDVSTDPYVGSVGKEAGFQSFM
                     SVPIKIEDKTIGTVNFHSNKRNKFGKREIELFSSIGTQIAIAVAKTKQTEDLQLVNED
                     LSVLNTIATSIHRSLDLNEVYKIALDAIIGITAFDIIMIYLVDENTNEAVLQAHRGLT
                     EDYIKRAERIPYPKGVTWKVIKSGELTHIDDIQKDPDLGPAGRALGHHTHVSVPIKHE
                     DKIIGVVNFASLRVLELSSRDINLLSAIGSQIGTAIVQAYLYEKSQEQREELGTLYED
                     LDKKNRDLEILSTITQAVHQSLDLEEIYKIALDMVTGMENVDMAMIYLVDDDRKEAVL
                     QTQRNFPEDYIRRAGRIPYPKGITWKVINIGEIVNIEDVQKDPDIGPAGRDLGHHSLL
                     GVPITLDGVVIGVTWFLSYKERQFNEQEVKLLSSIGNQIALAIAKAKLYRNLKEANEQ
                     LKKLDNMKDE"
     gene            complement(2122..2634)
                     /locus_tag="VI935_07715"
     CDS             complement(2122..2634)
                     /locus_tag="VI935_07715"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HLE25522.1"
                     /translation="MEFPIGKKISLGFGLALLVLSIISVFSYLSITEFIKTNDMQQNI
                     DDVSKNLERVFSQLKDVESSQRGYVITGQESYMEPYKVAIVSIDQGIKNLRELTADNP
                     NQRRRLNTLESMISEKLDFAKERIELRRNEGFEAAARKIKTGEGKRTMDGIRKVINEW
                     EKEQDLLLKQ"
     gene            complement(2784..3068)
                     /locus_tag="VI935_07720"
     CDS             complement(2784..3068)
                     /locus_tag="VI935_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLE25523.1"
                     /translation="MREWVLEEQNFAASIADVGSLALEASEWNRIEEALRKGEERYRA
                     LYEDNPFICFTVDLEGKVLSVNLFGAVQLGYKAHELVGQSVLLIFHTFLV"
     gene            complement(3495..>4016)
                     /gene="folK"
                     /locus_tag="VI935_07725"
     CDS             complement(3495..>4016)
                     /gene="folK"
                     /locus_tag="VI935_07725"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HLE25524.1"
                     /translation="IAFIGIGSNLGNRIENCVKGVEEISTFSKIIVVSSFYETEPVDK
                     EDQSDFINCMIKIETAHSPFELLTILNYCENKIGRKQVEKWGPRIIDLDIIFYDDLII
                     ETNDLTIPHPRAHLRRFVLQPLYEIAPNFIHPVLRKSVSELLSEVRCTKKVLKVGDFS
                     TYYSHKSTTCPQI"
BASE COUNT         1111 a          872 c          681 g         1352 t
ORIGIN      
        1 attcatcttt catattgtcc agcttcttaa gttgttcatt cgcctctttt aaatttctat
       61 acagctttgc ctttgctata gctaaagcaa tttgattgcc gatagaagaa agtaatttta
      121 cctcttgctc attgaattga cgctccttat aacttagaaa ccaggtaaca cctataacca
      181 ccccgtctaa agttatgggt acccccaaaa ggctatggtg tcccaggtct cttccagcag
      241 gacctatatc tgggtctttt tggacatcct caatgtttac tatctcaccg atgttaatta
      301 ctttccaagt aatgccttta ggatatggta tccttccagc cctgcgtatg tagtcttcag
      361 gaaagtttct ttgggtttgg aggacggctt cttttctatc atcgtctacc aaataaatca
      421 tagccatatc cacattttcc atacctgtaa ccatgtctaa ggcaatcttg taaatttcct
      481 ctaggtcaag ggattgatgt actgcttgag ttatagtact tagtatttct aagtccctat
      541 ttttcttatc taagtcctca tataaagttc ccaactcctc tctctgctcc tgggattttt
      601 catacagata tgcctgcact attgctgtcc caatttgact gcctatagca ctgaggagat
      661 ttatatctcg agaactaagc tctaataccc taagactggc aaaatttact actcctatta
      721 ttttatcttc atgttttata ggtactgata catgagtatg gtgacccaac gccctacccg
      781 caggaccaag gtcagggtct ttttggatgt catcaatatg ggtcagttca cccgacttaa
      841 taaccttcca tgttacaccc ttggggtatg gtattctttc tgccctctta atgtaatcct
      901 ctgtgagtcc tctatgtgct tgaagtactg cttcatttgt attttcatcc actagataaa
      961 tcataatgat atcaaaggct gttattccta taattgcatc tagagcaatt ttgtatactt
     1021 cattaagatc taaagaccta tgaatagagg tagctattgt atttaggacg gataaatctt
     1081 catttactaa ctgtaaatct tcagtctgtt ttgtctttgc tacggctata gcgatctgag
     1141 tgccaataga agaaaaaagc tctatttccc ttttgccaaa tttattcctt ttattactgt
     1201 ggaaatttac agtacctatt gttttatcct ctatctttat aggtactgac ataaagcttt
     1261 gaaaacctgc ttccttaccg acggaaccaa catatgggtc ggttgataca tcttgaacga
     1321 tataggtctc acctgagtta ataaccttcc atgttacacc cttagggtat tttatcctac
     1381 ctgctctttc tatgtattta tcagggtatc ctctatgagc ctcgagtacc gcttcatttg
     1441 tagcttcatc taccagatat attcctacaa tatctatatc cgttagttcc ataaccttgt
     1501 ctagagcgat attaaaaacc tctctaaggt ttgatgactt atgaaccgct tccgtaatag
     1561 tatttagtaa cctcacctct tcctctgaca gcttgcgcct cacgaggagc actacaatga
     1621 accagagcaa gcataccccc aataatcggc tgattaatgc aatttccagc ttaatacctg
     1681 ggggagagta gatgaagcca gtaatgagta atactgtgca gaccgtcgtg agaagaaaag
     1741 tgtatctttg ctgtgtgatc agagacatga taaacagcgg gaccaaatag agcccccaat
     1801 ccggtattcc ccgtggcgta aaaaggtcga ggacaaagat ccctgcgatc attacaacga
     1861 taatcgccag aataattacg ttaatggtct cctttgactt catggtaatc ttcctatgag
     1921 gtccattttg ggtgtcattg ccgttttcta cagcgcaacc ttcgctcttg agtgcgggtg
     1981 agacctacca tcacctgctt acgctcggtg aagtcactat ttatgataaa tattgccaaa
     2041 tcaacgatta tcaaagctat aaggttttcc aagaacgatt ataaacttcg cgttttgagc
     2101 acttgcttgc gtcgccaccg actactgttt caacaacaaa tcctgctcct tctcccactc
     2161 attaattacc tttcggatac cgtccatggt cctcttcccc tcacccgtct tgatcttccg
     2221 cgccgcagcc tcaaatccct cgttcctccg caattcaatc ctctccttcg caaagtcgag
     2281 tttttccgaa atcatggact caagggtgtt cagtctccgt cgttggttgg ggttatcagc
     2341 ggttaattcc ctaaggttct tgattccttg atcaattgat acgattgcaa ctttataagg
     2401 ttccatataa ctctcttgtc cagttatgac atagccacgt tgactggact cgacatcttt
     2461 caactgggag aacacccttt cgaggttttt ggaaacatca tcgatgtttt gctgcatatc
     2521 attagtctta atgaattcag ttatgcttag gtatgagaac acgctgataa tgctcagaac
     2581 taacaacgct aatccaaacc ctaaagagat ctttttaccg atgggaaact ccatattaat
     2641 acaccaaccc aatatttagt tttgctctat aggttaaact tttttcaata tatttctcta
     2701 caagcttaat aacggctctt ctcttttgcc ttctttttcc ttattcccct tccttttatg
     2761 tttttccgct tgcgctttct ctattatact agaaaagtat ggaaaatcag aagaacggat
     2821 tgcccaacta actcatgagc cttgtaacca agttgtactg ccccaaatag gttgactgat
     2881 aaaacttttc cttctagatc cacagtaaag catataaacg gattatcttc gtacaaagca
     2941 cggtaccttt cttcaccctt tcgtagcgcc tcttcaatcc gattccactc tgaagcctcc
     3001 aaagcgagag aacccacatc ggcgatcgag gctgcaaagt tttgttcttc tagtacccac
     3061 tctctcatcg gttatgtgta tttcccgctt ttaatttcag cttaaaaggg tggaaacatt
     3121 ctattttgat tgggtatatt tgctctgaga gaactaagca attgagttcg tatttgtcgt
     3181 agcttgacac catctcaaac ccattcttaa tacagtatta tttactataa tagcttattt
     3241 cgaatattaa aatcaagtca ataaggttag ttagttacta ttgtgttaat gctagtaatc
     3301 tctaaagtcg gtgtgtataa ccaataaaac gcatctttgg catatcttgt ggataacttc
     3361 gaagatatta tgtaaataag tatttattat tagcactatt tgtattaaga tggtgtaaat
     3421 aacgttgtat ttttaatgag ttatatttat agaaaaaaat tcctaattgt ggataactta
     3481 tggaaagaaa tgttctatat ttgtggacaa gttgtggatt tatgtgaata gtatgttgaa
     3541 aaatcgccta cttttaatac tttcttagta cacctaacct cacttagaag ctcagaaaca
     3601 gatttcctta ataccggatg tataaaattt ggggcaatct catatagggg ttggaggaca
     3661 aaacgtctaa gatgagccct tggatgagga atagtgagat catttgtttc gattataaga
     3721 tcgtcataaa atataatatc caagtcaatt attctcggac cccatttttc aacctgcttc
     3781 cttccaatct tattctcaca atagttaaga attgttaata gttcaaaagg ggaatgagca
     3841 gtttcaatct ttatcataca gtttatgaaa tctgactggt cttccttatc gacaggctcg
     3901 gtttcgtaaa aagaggaaac aacgataatc tttgagaaag tcgagatttc ctcaacacct
     3961 ttaacgcagt tttcaatcct atttccaaga tttgaaccta ttcctataaa ggctat
//
LOCUS       DATDRL010000226         5681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3219564, whole genome shotgun sequence.
ACCESSION   DATDRL010000226 DATDRL010000000
VERSION     DATDRL010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3219564"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VI935_07730"
     CDS             <1..184
                     /locus_tag="VI935_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PPOX class F420-dependent enzyme"
                     /protein_id="HLE25525.1"
                     /translation="GTAALTDNPDELLYWATRIGGRYMGAELAEAYGKRNSVEGELLV
                     RVTPSKIIAHKDVAGW"
     gene            1129..1749
                     /locus_tag="VI935_07735"
     CDS             1129..1749
                     /locus_tag="VI935_07735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HLE25526.1"
                     /translation="MDISDILTRRKFIGIITSFSVLSSLFPSNYLLSVWALENKNPLK
                     SQKLLKDKFKFFTPYQATVIEEVTSLIIPSDNSPGAREAGVVFVIDNSMSENNRLQTL
                     YLQGIEWLDLMSKMLSGENSFLDLNQDEMIEILNIADQKKSLLYKEANIPRVDRDKNI
                     GSLFFDNLVRQTFNIFYTSDLGWKVVGYHGPPQWAGNLNYNKCNLK"
     gene            1746..3434
                     /locus_tag="VI935_07740"
     CDS             1746..3434
                     /locus_tag="VI935_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLE25527.1"
                     /translation="MSVEKEVDVCIIGSGAGGAPVAYALGKAGFSVLVLEAGRRYNPN
                     EYHLAKKDWERYPYPQPFVDPKLGSKKDLYTYSDPEELNPNYKKLRSWSKAQGHLNKS
                     DRRRPPHIDRAKGVGGTTLHYQGEAHRFSPFGFKSESLYGIGDDWPITYQDLEPYYDK
                     AEKLLGVAGMDGNPFKPQRGQFPNPPHELSCASKRVKVGFDKLGLHLWPNSLNINSRP
                     FDGRPACNYCNGCFLGCMMRAKGSMDVTLIPKAEATGNVEVRSNSVVREITINKKGKV
                     DGVIYFDSNKKEKKQMAKIVVVSASALESPRLLLNSKSSLFPDGLANSSGLVGKNFME
                     TIIYSNAVLFAEQIDSYKGLQIDSRAWDYNETRKDNSYIGGVVFGVSALELLGPLNYA
                     QRIAPGWGGEHKDFMTKYFGHAVNVFAIGEQLPNEDHMITIDSEVKDYYGIPVAKITT
                     KLSNNDLEMLSFMQRKCNEIVEAAGVVEKVREFGAYDLSLISHMGGTSRMGVDPKKSV
                     LNSYCQSHDIKNLFVIDSSCFVTQGGGDSPSLTIHAIALRASDYIISESKKGNL"
     gene            3461..3676
                     /locus_tag="VI935_07745"
     CDS             3461..3676
                     /locus_tag="VI935_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25528.1"
                     /translation="MRPEALFANFELLLFAGHETTRNLIGNAVNLLLTHPSEFQKAKE
                     APSLWKNVVDETMRFDPPDTDDLPNSG"
     gene            3636..3992
                     /locus_tag="VI935_07750"
     CDS             3636..3992
                     /locus_tag="VI935_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HLE25529.1"
                     /translation="MTPPIQMISRIAGDDIEVHGKQIRKGQQLFMVLASGNRDPDHFP
                     YPDHFDIEREAGGHLAFGLGPHYCLGSLLAEAAAMIALETLFERFPELKLDPTRKKVW
                     VINTRFHGLQSFPVVR"
     gene            4146..4586
                     /locus_tag="VI935_07755"
     CDS             4146..4586
                     /locus_tag="VI935_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25530.1"
                     /translation="MIRFRNIFPIVVFTLLVFMTVNTMGASPMDQAKTELDTAIYHGK
                     ASVDSDSAGAMKAHLHHAINCMEGPQGQNFDSSEVNPCKGQGNGIVPDLKAAGSPASK
                     ALKHVQEADNIAVTSLGVSDINTLRNNINEVVNHLEQAKAELGM"
     gene            4774..5385
                     /locus_tag="VI935_07760"
     CDS             4774..5385
                     /locus_tag="VI935_07760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HLE25531.1"
                     /translation="MIRILALGDSYTIGEGVNQSESWPALLAGLLREANLKVDTPQMI
                     AVTGWTTIDLISAIGKSRLEEQYDLVTLLIGVNDQFQGYSEESYATNFEKLLIKAIEL
                     AGGRPYRVIVISIPDYSVTPFGKRFGSARIRAEIERFNEINNKIAVEKGVRYVNVTEI
                     SRRASEDLTLLAPDGLHPSAKMYAEWVELIAPVARSMLEESNN"
     gene            5446..5562
                     /locus_tag="VI935_07765"
                     /pseudo
     CDS             5446..5562
                     /locus_tag="VI935_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
BASE COUNT         1770 a         1046 c         1277 g         1588 t
ORIGIN      
        1 aggtacagca gccttgacag acaatcctga tgagctgctt tactgggcta ctcggatcgg
       61 aggcagatat atgggtgctg aattagctga agcctatggc aaacgtaata gtgttgaagg
      121 cgagttgctg gttcgagtta ctccatccaa aattatcgct cataaggacg tcgccggttg
      181 gtaaaaattt taaaagctca ctagtgctag accagataat tttgaaccag gagcttacgc
      241 aagagcgccg ttcatggttc gacaagcctg ccctgagtgt agccgaaggg ctcaccacga
      301 acggcttagt ctaaataccg ttcgccctga gctcagtcga aaggcgtgct ttattggata
      361 aatgttagag ttaatttagt ggtgcactag ttttatacct tgacgaattc gatcaattcg
      421 tttaatgcga agtcgtttca ttgcatttac ttcttatcaa tttgttttac gctggcgctt
      481 tatttatatt atcgaaaacg atggagcgta atatgtctga aaaccatcct ttagctagcg
      541 atgaccaggc attaaatcac ttagcaaaag catttgagaa agccgcacat gagctgtata
      601 tgcaacccat tgtagaggcg cattgaaatg tgtctctaca tattgcctac cctgttaggt
      661 tattacccgt ttaatgaatc aaatgtcatt aggattccat tctaaactct aacctgcgac
      721 aaaaggtgtc tgttgtccct attttaacca ttgggaacaa gaatgtcccc tctatcgtag
      781 gaaagtttaa aatatgccga taggcagggt tttctaaccc tgcataatat tgggactcga
      841 aaaccggaca tatctgaaaa aataagatag agacgacccg ccgggtcgtc tctacaataa
      901 ttcaacgggc tcataaaaat ctttagggta ggggctgatg ccctcatcag cctcccgatg
      961 aagtcgtcgg ggctagttcg gggaacaggc gctacatcta aaaacttgaa ggaatttggg
     1021 catctttgct gaaggataag taagaataga tttcactata attcattgaa aaatagtatt
     1081 tgaccaatcg atgttgtaag attatcctgc taatacaagg ctatctcagt ggacatttca
     1141 gatatcttaa caagaagaaa attcatcggt attataacct cattctcggt tttatcatca
     1201 ttgtttccat ccaattactt actttcagta tgggcattag aaaataaaaa ccctttaaaa
     1261 tctcaaaaac tacttaagga taaatttaaa ttttttactc cttatcaagc aacagtcatc
     1321 gaggaagtaa cgtcattaat aattccaagt gataatagtc caggtgctag ggaagcgggt
     1381 gtagtttttg taattgataa ttcgatgtcg gaaaacaata gactgcaaac actttatcta
     1441 cagggaatag aatggttgga tttaatgtca aaaatgctat ccggtgagaa tagctttctc
     1501 gatttgaatc aggatgaaat gattgagatt ttaaatatag ccgatcaaaa aaaatcttta
     1561 ttatataaag aagctaatat tcccagagtg gatagagata agaatattgg aagcttattt
     1621 tttgataatc tcgttcgcca aacgtttaat attttttaca caagtgattt aggatggaaa
     1681 gtcgttggtt accacggacc acctcaatgg gcggggaacc ttaattacaa taagtgtaat
     1741 ttaaaatgag cgtagaaaag gaagtagatg tttgcataat aggttctgga gctggtggag
     1801 cgcctgtagc ttatgcgctt ggtaaagccg ggttctctgt gttagttctc gaagccggtc
     1861 gtcgctataa tccaaatgaa taccatcttg ccaagaagga ttgggagcgg tatccctatc
     1921 cccaaccatt cgttgatcct aagcttggta gcaagaaaga tctttataca tacagcgatc
     1981 cggaagaact taatcctaac tataagaaac ttcgttcctg gtctaaagcg caaggtcatt
     2041 taaataaatc agatagaaga agacctcctc acatagatag ggcaaagggg gtaggaggaa
     2101 cgacactgca ctaccaggga gaggcacacc gattttcacc cttcggtttt aaatctgagt
     2161 cattgtatgg aataggtgat gactggccca tcacttatca agaccttgag ccctattatg
     2221 ataaggcgga aaagctactt ggagtagcag gaatggatgg gaaccccttt aaaccccaaa
     2281 ggggacaatt ccccaatccc ccacatgagc ttagctgcgc cagtaagagg gtcaaggttg
     2341 gatttgataa gcttggtctc catctctggc ccaattcact gaacatcaac tcaaggcctt
     2401 tcgatgggag gcctgcatgc aattactgca acgggtgttt tcttggatgt atgatgaggg
     2461 caaaggggag tatggatgta acactgattc caaaggcaga ggcaactgga aacgtggagg
     2521 ttcgatcgaa ttcggtggta agagaaatta cgataaacaa aaaggggaag gttgatggag
     2581 taatatactt tgactctaat aagaaagaaa aaaaacagat ggctaaaatt gtagttgttt
     2641 ccgcgagtgc cttagagtcc cccaggttgt tattaaattc aaagtcatca ctctttcccg
     2701 atggcctagc aaacagcagt ggtcttgtgg gaaagaactt tatggaaaca attatttata
     2761 gtaatgcagt tttattcgcc gagcagattg attcctataa ggggttgcag atcgatagcc
     2821 gtgcctggga ttataatgaa acacgaaagg ataattccta tataggtggc gttgtatttg
     2881 gtgtttctgc cctggagtta ttaggtccac ttaattatgc gcaaagaata gcccctggtt
     2941 gggggggtga gcataaggat tttatgacca aatattttgg acacgcagtt aatgtatttg
     3001 caataggaga acaattgcct aatgaggatc atatgataac aatcgattcg gaagttaagg
     3061 attattacgg tattccggtt gcgaaaatta cgactaaatt gagcaataat gatctggaga
     3121 tgttgtcatt catgcagaga aaatgtaatg aaattgtaga agctgctggc gttgttgaga
     3181 aggtcaggga gtttggggca tatgatttga gtctgatttc tcatatgggc gggactagta
     3241 ggatgggtgt cgatcctaaa aaatccgttt taaattccta ttgccagagt cacgacataa
     3301 aaaacctctt tgtaattgac agtagctgct ttgtgactca aggcggagga gatagccctt
     3361 cccttaccat tcatgccata gcccttagag cttcagatta tatcattagt gaatcaaaga
     3421 agggaaacct ataaatgtaa acttattggc tggccatgtt atgagaccag aagccctttt
     3481 tgcgaatttt gaactattac tttttgcagg acatgagacc acaagaaacc tgattggcaa
     3541 cgccgttaac ctgcttttga cccatcccag tgagtttcaa aaagcgaaag aagccccttc
     3601 tctttggaag aatgtagtag atgagacaat gcgctttgac ccccccgata cagatgatct
     3661 cccgaatagc gggtgacgat attgaagttc atggtaagca aatacgaaag ggtcagcaat
     3721 tatttatggt tttggcttcc ggcaatcgcg atcccgacca tttcccatac ccagaccatt
     3781 ttgacattga gcgagaggcg ggtggacacc tagcttttgg cctgggacca cattattgcc
     3841 tgggttccct tctagcagag gcggcagcaa tgatcgccct tgaaaccctt ttcgaacgtt
     3901 ttcccgaact aaagcttgat ccgacgcgga aaaaggtctg ggtaatcaat accaggtttc
     3961 acggattgca gtcttttcca gtggttcgat gaatgttcgt ggacgttagg tgacaagcta
     4021 tttattcatt ttatttttga catcaacctt atagttttgt actcttttta acgataaaat
     4081 gattatacta cacgcttgaa tgacgtgagt agcttaacaa aaaataaaga ggaggagaga
     4141 gaaaaatgat aagatttcga aatatatttc caatagtggt atttaccctt ctagtattca
     4201 tgacggtaaa tacaatgggt gcatcaccca tggatcaggc taaaactgag cttgatactg
     4261 cgatatacca tggaaaagca tctgttgact ccgattcagc gggtgcaatg aaggcgcatc
     4321 ttcaccatgc tataaactgt atggagggac ctcagggaca aaactttgat tcttcagagg
     4381 taaatccttg caagggtcag ggtaatggca tagttcccga tttaaaagcg gctgggtctc
     4441 cggcatctaa agcattgaag catgtccaag aggccgataa tatagcagta acttcgttag
     4501 gagtatctga cataaatacg cttcgaaaca atataaacga ggtagttaat catttagaac
     4561 aagctaaagc ggaattgggt atgtaaaaat ctattcaaga ctcactgatc tcaaagtaga
     4621 attctggtta aatcttaaca aatattaatc atgcaacgta ctctgtctat cctgagtatt
     4681 tctttattcc ttagtatttt taaaatttct tgccttagtt cgtcatatca agacagaaca
     4741 aagatggagc caaccaatga agaggaatct caaatgataa ggattcttgc actcggggat
     4801 tcttatacta tcggcgaagg agtcaatcag tcagaaagct ggccggcgct gcttgcagga
     4861 ctgcttcggg aagccaacct aaaagttgat actccacaga tgattgctgt caccggttgg
     4921 actacaattg acctcattag cgcaatcggg aaatcaagat tagaagagca atatgacctt
     4981 gttacattgc ttatcggggt aaatgaccaa ttccagggtt atagcgaaga aagttacgct
     5041 actaattttg agaaattact tattaaagca attgaattag caggaggaag accttatcgg
     5101 gtcattgtaa tatcaatacc tgactatagc gtgacaccat ttggcaagcg gtttggttct
     5161 gctaggatta gagctgaaat agagaggttt aatgagatca acaataaaat tgcggttgaa
     5221 aagggtgtcc gttacgtaaa cgtaacagaa atatctcgaa gggcttctga agatttaact
     5281 ctattagctc ccgacggtct ccacccatca gcaaagatgt acgcagaatg ggtagaattg
     5341 atcgcaccag tagctagaag tatgcttgaa gaaagtaata attgataaat aagatgttac
     5401 acccctttct gtacaaatct ttagggcttc gaatacacag aggaatttac agttgaaaag
     5461 ctaatgaggg atgctaaaat aatgcagttt tacaaaggcg ccaatcaaat tcaaggattg
     5521 gctaccgcaa gagaaatttt aaacactact aaaataagat aaggaggaaa gacacagtga
     5581 agatcgttgt tattgtgaga caaacccctg atacagaggc gaagattaag gttgcctcga
     5641 gtggaaattc aattgatatt gaagggataa aatggattgt a
//
LOCUS       DATDRL010000227         5870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1601459, whole genome shotgun sequence.
ACCESSION   DATDRL010000227 DATDRL010000000
VERSION     DATDRL010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601459"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VI935_07770"
     CDS             <1..223
                     /locus_tag="VI935_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25532.1"
                     /translation="VAKQLNPPLDKHTIWGGIKPHIETKLKETQEQVFKTHRREGFDR
                     VAKILLYMEKLGLPEEAKEATPKWLEKLK"
     gene            complement(268..1998)
                     /locus_tag="VI935_07775"
     CDS             complement(268..1998)
                     /locus_tag="VI935_07775"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HLE25533.1"
                     /translation="MTKSALKALILIFVQTIALTWAYELGFAQDLNVQVLSEQVREHL
                     QNRIETEFVLPDTAIEDEYTYTTSMLSRFYGRRNYLPAWISNDGPLTQVDALIKTIRE
                     ANQEGLMPDEYNLGKIEDKLREIRQRKDTNTPIDPFTLVILDILLTEAFLTYSSHLLD
                     GRVNPEKIEASWYPNRYEVDLVLLLQAALESNTVEESLRSLLPVDQRYIRMRQALRRY
                     RDIAARGGWNSVPDGPKIKKGDSGARIQALRARFITEGYLVPGLSKNSEIFDDQLEQA
                     ISKYQDNHGLKVDGIVGRDTLASLNISVEERVRQIELNMERLRWLPKDLGKRYVLINI
                     PNYQLEVVENGKPVMIMRAVVGKPSDPSPVLSSEITYLVLNPSWYVPSSIATSEILKE
                     LRTDSTYLAKNDIKVYKVEDGTRREINPATVNWTQVSEKNFNYKFRQRPGSKNVLGRI
                     KFIFPNPFDVYLHDTSSPELFAKFVRSFSHGCMRVEKPIELAEFLMRDDPEWSRESIL
                     SAIDKRSEKTVWLPEPIPVNVVYLTAWVDENGSVQFRNDIYGLDKVLNEALNQRDKAL
                     SISKESLNHE"
     gene            complement(2243..2485)
                     /locus_tag="VI935_07780"
     CDS             complement(2243..2485)
                     /locus_tag="VI935_07780"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLE25534.1"
                     /translation="MFDPIWSSRRHLITVYPLGGCPMGDGDTKGVVDADGQVFNYPNL
                     YIADGAIVPSALGPNPSKTIGTLAERISEKIIGKGM"
     gene            complement(2771..3295)
                     /locus_tag="VI935_07785"
     CDS             complement(2771..3295)
                     /locus_tag="VI935_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HLE25535.1"
                     /translation="MKYTAFVLVIPLLIIWVVLNAYAEPNKAKAELNNSKGENIGIAT
                     LVEGIDGVLITINVWNLPPGLHGFHIHEAGKCEPPDFKTAGGHYNPYGRKHGLKNPEG
                     QHSGDSPNLLVGPDGTGTQVILFPLVTLGDGTNSLFHQGGTALVIHASADDQITDSSG
                     NSGARIACGVISKY"
     gene            3648..4913
                     /locus_tag="VI935_07790"
     CDS             3648..4913
                     /locus_tag="VI935_07790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLE25536.1"
                     /translation="MSRGIFYGWYIVAASLVLIIMDGLLLYSFGVFLPYLNEEFGFSR
                     AIGSSIFSLRSVVLAFSLTLAGRLVDKYDPRAVIISGGIITAIGMLLSGFADKGWELY
                     VSYGFLIGLGDGVLYITCVAVVSRWFIKKRALVIGIITTGIPLSGLIASPLTAWLISS
                     FGLRNAFFALAGLLTLSTLSALALRGYPQEKNLKPYGEEEEPSTRTVQVTRDGLKNNN
                     NDWKALEAIATPVFWLMYSMYFLGFTTFLVVVIHTFSFAVDLGIPALVASGALSAIGI
                     GSILGRVALSGLLTEVLETKKVLFLCFFLQGSSLLLLLGVREVWAFYLFGVLFGFFYS
                     GLVPIFPTLLGKFFGLSAMGTIYGFFGTSFCVAAIGGPLLAGYIHDITGSYFYPFLLS
                     IFFCYAGAILSFFIKSPVRNIKPIESISQ"
     gene            5203..>5870
                     /locus_tag="VI935_07795"
     CDS             5203..>5870
                     /locus_tag="VI935_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25537.1"
                     /translation="MNRDLIGEIRQYGERLLRELNEELYRNLAGLKKESNISPIYKSY
                     QDLLDPGVFFSINSVSPKYNEEERGIKLLRGFLAKSIIERKTSTLKDKILTIETRAEI
                     PLGSRGISYRSASAEIKREPRREIREEIDEKRCGIALKLNPLFLEALYTTHKTSSELG
                     FLYTNLCDEIEGLNLNQLETNARLFLKDTEYIYRDLLKWFLLKRMELKLQDAKKHDLD
                     FLFNS"
BASE COUNT         1696 a         1181 c         1177 g         1816 t
ORIGIN      
        1 ggtggcgaag caactaaatc cacctctcga caaacatacg atttggggag ggataaaacc
       61 tcacatagaa accaagctaa aagagaccca agagcaagtg tttaaaaccc atagaagaga
      121 ggggtttgat agggttgcta aaatcctgct atacatggag aaactcggtt tacctgagga
      181 agccaaagag gcaaccccta aatggttgga aaaactaaaa taggaatcga aatctccaag
      241 ctcctagaga acccgcaaat tcgtgtgcta ctcatgattc agggattcct tgctaatgga
      301 aagagcttta tcgcgttggt tcaaggcctc gttaagcacc ttgtctagtc cgtagatatc
      361 attacgaaat tggactgagc cattctcgtc tacccatgcc gtcaagtaaa ctacgttgac
      421 aggtattggt tctggcagcc acacagtttt ttctgaacgt ttatcgatcg cactgagtat
      481 actttcgcgt gaccattctg gatcatctcg catgagaaac tctgcaagtt ctataggctt
      541 ttcgacacgc atgcagccat gactgaaact tcgtacaaat tttgcaaaca gttctgggga
      601 ggacgtgtcg tgaaggtaga catcaaatgg gttaggaaaa atgaacttta tacgaccaag
      661 cacatttttc gagcctggcc tctgcctaaa tttataattg aagttttttt cgctaacctg
      721 agtccagttg acggtagccg ggttaatttc tctcctagta ccatcctcaa ctttataaac
      781 ctttatatcg ttcttagcaa gataagttga atcagttcga agttccttca atatttcaga
      841 ggtagcaatg cttgatggga cataccatga aggattcagc actagatatg tgatttcact
      901 gctaaggact ggactagggt cagatggttt acctacaact gcccgcataa tcattacagg
      961 tttaccattt tcaaccactt ctaattgata attagggatg ttgataagta cataacgttt
     1021 ccctaggtct ttagggagcc agcgcaagcg ctccatatta agctcaatct ggcggactcg
     1081 ctcctcaact gaaatattaa gtgatgcgag agtatcacga ccaacaatcc cgtcaacttt
     1141 tagaccgtgg ttatcttggt atttgctaat tgcctgctct aactgatcat cgaatatctc
     1201 actattctta cttaaacctg ggacaaggta accttcagta atgaatcggg ctcgcagtgc
     1261 ttgtattcta gctccactgt cgcccttttt tattttcggt ccatcgggta ctgaattcca
     1321 cccgcccctt gcggctatat ctcgataacg ccttaaggcc tgtctcattc ttatgtagcg
     1381 ttgatctact ggaaggagac tgcgcaggga ttcttccact gtatttgact caagggcagc
     1441 ctgaaggagg agaacaagat ctacttcata tctgttgggg taccaagatg cttctatctt
     1501 ttctggattt actcgaccat ccaataaatg ggaactataa gtcaagaagg cttctgtaag
     1561 cagtatatcg agaataacta atgtaaatgg atcaatagga gtattagtgt ctttcctttg
     1621 ccttatctct cttaatttat cctcgatctt accaagattg tattcatctg gcattagtcc
     1681 ttcttgatta gcttcacgaa tagtttttat aagagcatct acttgggtta gggggccatc
     1741 attgctaatc caggcaggca ggtagtttcg ccgcccgtag aaacggctga gcattgaagt
     1801 tgtatatgta tattcgtcct cgattgctgt atccggcagt acaaattctg tttctattct
     1861 attttgtaga tgttcacgaa cttgctcaga taaaacttgc acattcaggt cttgtgcgaa
     1921 accaagctca taggcccagg ttagggcaat agtttgaaca aagattaaaa ttaatgcttt
     1981 tagagcagac ttagtcatat tttaattaga tgcaaaattc ataccactgg ttttacaata
     2041 aaaatatata tgtaacttat tgcaatcatt ataaatagtt taaggacaga acataccttg
     2101 taacacgtat ttgacaaaaa ttgttatttt tcgtgaagaa aatgtcacag gatgacaaat
     2161 tttgtgaaat ttaaataatt atttaggact tgagaataaa ctataacctt agaatatcta
     2221 aaagcaacct ggttctttca atctacatgc ctttcccgat aatcttctct gaaatccttt
     2281 cggctagggt gcctattgtc tttgaagggt taggtcctaa agctgagggt acgattgccc
     2341 catcggctat gtataaatta ggataattga aaacctgacc gtctgcgtca acaacaccct
     2401 ttgtatcacc atctcccatt ggacaaccgc ccaagggata cactgtaata agatgacgcc
     2461 tagaagacca gattgggtca aacattaaat tcccatcaag tccgccgttc gtaactgtat
     2521 atgagcagca tcagcaaaaa tggttccaat agcgaatgcg actggacatc tatctgtacc
     2581 atatcacccc tccattttct tgatgcttca taatactaga actggataac ataggttgat
     2641 atcacagaag acgtaacgta atcacacaac aatcacctag tttttagaac taagctaaac
     2701 cgcgagtcct gttaaacaaa ccagtttttc tttctatgta aagtaagaaa tttttaaagt
     2761 caaggttttt tcagtactta gatataacac cgcatgcgat acgagcacct gaattcccac
     2821 tagagtctgt tatctggtca tctgccgaag cgtgaatgac aagagccgta ccaccctgat
     2881 gaaatagaga gttggtgcca tctcctaagg tcacaagggg aaatagtata acttgagtgc
     2941 cagtgccatc gggtcctaca agcaggttag gcgaatcccc gctgtgttgc ccttctgggt
     3001 ttttaagccc gtgctttctt ccgtatggat tatagtgtcc tcccgctgtt ttaaaatcag
     3061 gcggctcaca tttcccagct tcgtgaatat gaaaaccatg aagacccggt gggagattcc
     3121 acacattaat tgtgatcaaa acgccgtcta ttccttcaac aagtgttgct atgccaatat
     3181 tctctccctt agaattgtta agctcagcct tagccttgtt tggttctgca tatgcattca
     3241 aaacaaccca tataattaaa agtggtatta ctaaaacgaa cgctgtgtat ttcatctttt
     3301 taatacctcc taggatattt gtataattta ggggaccccc tggattcacc ttttcaaaaa
     3361 taccactagt ttttaataaa gaaaagataa tttttaatga gatatgttag gatactagca
     3421 gatcaagcaa ctttatttca ttcgtggata tgtacaactt aagatcgtca gctatcaggg
     3481 cttctggcat tgatacaaag tgtttatcgt gtccccattt cttcgatatt ttcaggtatg
     3541 attatttttc ttgagtttta taatctgatt gttgcttata ttcacagttt ggggttcaaa
     3601 tcgtcttgta aatttagccc gcattgaaag taagattagg agaaaatatg tcccgaggga
     3661 ttttctatgg atggtatata gtagcagcct ctcttgtttt aatcataatg gatggccttc
     3721 ttctctactc ctttggtgtg tttcttcctt acttgaatga ggagtttggt ttttctcgtg
     3781 cgatcggttc ttctattttt tctttgagat cagtggtact tgccttttct ctaaccctcg
     3841 ctggacgact tgtagacaag tacgacccaa gggcagtgat tatttcaggt gggattatca
     3901 cagcaattgg catgcttctc tctggatttg cagacaaggg atgggagtta tatgtttctt
     3961 atggatttct aatcggtctt ggagacggag ttctatatat cacatgtgtt gcagtagtta
     4021 gtagatggtt tataaagaaa agggcgcttg taataggaat tatcacaact ggcattccac
     4081 taagtggtct aattgcaagc ccgcttaccg catggcttat ttcttcgttc ggactgagaa
     4141 acgcattctt tgctctggcc ggacttctta cactttctac tctctcggca ttagcattaa
     4201 ggggataccc tcaggagaag aatctaaaac cttatggtga ggaggaagaa ccatctacga
     4261 gaacagttca ggtcactcgg gatggactaa aaaacaataa taacgattgg aaagcacttg
     4321 aggctatagc tactccagtt ttttggctaa tgtactcgat gtattttttg gggtttacca
     4381 cctttttagt agttgtgatt cataccttta gctttgcggt agatttaggg attcccgctc
     4441 ttgtagcctc tggtgcgcta tccgctatcg gaatagggag catactagga agagtagctc
     4501 tttctggact tcttacagag gttttagaaa ccaagaaggt tctttttctt tgtttcttcc
     4561 ttcagggaag ttcattgttg cttctactag gtgtaaggga agtatgggct ttttaccttt
     4621 ttggcgtcct atttggattt ttctatagtg gacttgttcc cattttcccc acccttcttg
     4681 gaaagttttt cgggctaagt gcaatgggga caatatatgg cttctttggt acctcttttt
     4741 gtgtagctgc tattggaggg ccacttctcg caggatacat tcacgacata acaggtagtt
     4801 acttctaccc atttcttctt agcatatttt tctgctatgc aggtgctatc ttatcctttt
     4861 tcatcaagtc tccagtaagg aacattaaac ctattgaaag catttctcaa tagtgtgggt
     4921 gttctgaaag gatttggatt cccgcttaaa acatgcggga atgacaatcc actagtcaac
     4981 aaactcaata cggatggtag tggaaattaa ccaatctaga accgcccttt tgcaagtagg
     5041 cggttctcta actgcctccc tctagatagt agggcacaac gaagcgtgcc cctacatgtt
     5101 cctatcaaag acaaaataat taggtctttt tatcaaaaac atatattgta gtttatttaa
     5161 tgtttcaaaa tgaaaactgt ataagatata ttaaagcggt cgatgaatag agatctaata
     5221 ggagaaattc ggcaatacgg tgaacgatta ctcagggaat taaacgagga gctttatcga
     5281 aatttagccg gattaaaaaa ggaaagcaat ataagcccga tttacaaatc gtatcaagat
     5341 cttttagacc ctggcgtctt cttttctatc aatagtgtat cacctaagta caatgaggaa
     5401 gaaagaggga ttaaactcct tcgtggtttc ttagcaaaat ccattataga aagaaaaacg
     5461 tcaactctga aagataagat tctaacaatt gagacgagag ctgaaattcc actcggctca
     5521 agaggaattt cttatagatc agcctcagca gaaataaaga gagaaccaag aagagagata
     5581 agagaagaga tagatgaaaa aagatgtggg atagctttaa aactcaaccc actttttctt
     5641 gaagcattat atactacgca caaaacgtca tcggagctcg ggtttttata cacaaacctt
     5701 tgtgatgaaa ttgaagggtt aaacctaaat caactcgaaa caaatgctag actatttctc
     5761 aaggatactg agtacattta ccgcgattta ctaaaatggt ttctcttaaa acgaatggag
     5821 cttaaactcc aggatgccaa gaagcatgac ctcgattttc tcttcaactc
//
LOCUS       DATDRL010000228         2885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1448372, whole genome shotgun sequence.
ACCESSION   DATDRL010000228 DATDRL010000000
VERSION     DATDRL010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448372"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VI935_07800"
     CDS             <1..309
                     /locus_tag="VI935_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25538.1"
                     /translation="AAATDAALGRRNTEFQAAAGALDANSVDLSKAIGSVYGPDAEKA
                     FLPLWRKHIGFFIDYTVGVATKDKAKQEKAVSDLVQYPKDFGAFMHSASPAIPANVVG
                     "
     gene            299..484
                     /locus_tag="VI935_07805"
     CDS             299..484
                     /locus_tag="VI935_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25539.1"
                     /translation="MWADLVKTHVFTLKAVVDAQAAKNWAQADSDIRTAASHMQMIAD
                     PLAEAIINQFPTKFAAK"
     gene            557..958
                     /locus_tag="VI935_07810"
     CDS             557..958
                     /locus_tag="VI935_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HLE25540.1"
                     /translation="MKMKVAFKFFSILIMTPLFIFGPSFYLGETEADETKSQSSKQSN
                     ENIDIKLFTFEPETIEVPVGTTVVWINFDAIEHSVTNGTPEQPGKTFDSDFFTEGQNF
                     TFTFVKPGTYPYFCKRHNSMRGEIKVLPATP"
     gene            complement(1069..1809)
                     /locus_tag="VI935_07815"
     CDS             complement(1069..1809)
                     /locus_tag="VI935_07815"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HLE25541.1"
                     /translation="MSHARLRIFVIASVIIHILAFSIWAFVWRPEVIVTNAELIDLGV
                     IGASKEHKAGGGNIFVPSQKAASKVENAVIKPITRKNQEKEITNKREASIQHSKLMIE
                     PSTVNKSDYTNKEQTHLISNTSKASAESLKEGTQIGYERLSVLGTDGSGQAVEVGYPD
                     YKINPKPGYPIIARRNGYEGAVLLKVWVLEDGKVGKIEIEQSSGHEILDKSAIEAVTN
                     WIFMPGKRNGVPLPSWVTVPIKFQLRGG"
     gene            complement(1850..>2885)
                     /locus_tag="VI935_07820"
     CDS             complement(1850..>2885)
                     /locus_tag="VI935_07820"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLE25542.1"
                     /translation="IPETEAQIHTELIREKSPTANVIGYIEGSDQSLKDQVIIIGAHY
                     DHIGLGDRVSRGDDKTDKNKVHNGADDNASGVAGLLELAEYFSDQRVSLKRSLLFIAF
                     SAEELGLIGSSYYVKHPKIPLGNTLAMINMDMIGRLRDDKLTVFGAGSSPEWKPLLES
                     FNSNLGLTSQTKDSGFATSDQTVFYANDIPVLHFFTGVHEDYHAPGDDWQKINSEGEK
                     KILLLISDLIGKLNETSTKIALSKAKEEERAPSQFNVYVGTIPDYSEEGEGVTLMGVK
                     EGSPADKAGLKGKDTIVEFGGQSIKNIYDYVYALGEADPEVPTEIVILRKGKRIKLSI
                     IPGSRKDKIN"
BASE COUNT          803 a          642 c          552 g          888 t
ORIGIN      
        1 gctgcagcaa cagatgcggc cttaggcaga cgcaatacag agtttcaggc agcagcggga
       61 gcccttgatg ctaactcagt agacctttcc aaagcaattg gatctgttta cggtcccgat
      121 gctgaaaagg cgtttttgcc cttgtggcgt aaacacatcg ggttttttat cgattacaca
      181 gtaggtgttg ctacaaagga taaggcaaag caggaaaaag cggtgtccga tttagtacaa
      241 taccccaagg atttcggtgc ctttatgcac tctgctagcc cagccattcc cgcaaacgtt
      301 gtgggctgac ctggttaaga cccatgtttt tactctaaag gcagtggttg atgctcaagc
      361 agctaagaat tgggcgcagg cagattctga tataaggact gctgcaagcc acatgcagat
      421 gattgccgac ccacttgctg aggctattat caatcagttc cccactaagt ttgccgctaa
      481 gtaatgttaa gttttagaca agtgtaatag agcaagagga attcctacct cttgctctat
      541 tttttaggag gcatagatga aaatgaaggt agcattcaag ttcttttcaa tattaataat
      601 gacaccgctt tttatttttg gacctagctt ttatcttggt gaaaccgagg cagatgagac
      661 aaaatcccaa tcatctaaac aatcaaacga aaatatagat atcaagttat tcaccttcga
      721 accagaaacg attgaggtgc ctgttgggac tacagttgtg tggattaatt tcgacgctat
      781 cgagcacagt gtgaccaacg gaacaccaga gcaacctgga aagacctttg attccgattt
      841 ttttacagag gggcaaaact tcacgtttac ctttgtaaag cctggcactt acccctattt
      901 ctgtaaacgt cacaactcga tgcgcgggga gattaaggtc ctacccgcaa ctccttaaga
      961 tcaaggcttt ataagtaagt attcaaatat tgtaggcaca gacttgtctg tgcaacacta
     1021 taattttgac tggggaaatg ttataaggaa tagccaaaat ctacgatttc atccacccct
     1081 aagttgaaac ttaatcggaa ctgtaaccca actgggtaac ggaaccccgt tcctttttcc
     1141 aggcataaaa atccagtttg ttaccgcttc gattgcggat ttgtcgagta tttcgtgacc
     1201 agaggactgt tcgatttcaa ttttacctac cttaccatcc tcaagcaccc ataccttgag
     1261 aagaactgca ccttcgtatc catttcttct tgcaatgatt ggatacccag gcttagggtt
     1321 tattttataa tctgggtaac ctacctcaac agcctgccct gagccatcgg taccaagcac
     1381 agatagtctt tcatatccta tttgcgttcc ttcctttaaa gactctgcgc tagcctttga
     1441 agtatttgaa atcagatgtg tctgttcttt atttgtgtaa tcacttttat taactgtact
     1501 tggttcaatc attaatttag agtgttgaat agaagcctct ctcttatttg taatttcctt
     1561 ttcttgattc ttccttgtga taggtttgat aacggcattc tctactttcg atgccgcttt
     1621 ttgacttggt acaaaaatgt taccacctcc tgccttatgc tctttggagg caccaattac
     1681 accaagatct attagttctg cgttggtaac gataacctcc ggccgccaca cgaaagccca
     1741 gatagagaaa gctaagatgt gtataatcac tgaagcaata acaaatattc taagacgagc
     1801 atgagacata aaaacatctc cagctaaaca gagtttattt ctaataattc taattaattt
     1861 tatcctttct tgaacctggt ataatgctta acttgatccg tttgcctttc cttaatataa
     1921 caatctcggt tgggacttct ggatccgcct cccccaaagc gtatacataa tcatagatgt
     1981 tctttataga ctgtccacca aattccacta ttgtgtcttt accctttagg cctgctttat
     2041 cagcagggct cccttctttt acacccataa gtgttacccc ctctccttcc tctgaataat
     2101 ctggtatcgt tccaacatag acattaaatt gagagggtgc tctttcttct tctttagcct
     2161 tagacaacgc tatcttagtc gatgtttcat tcagcttgcc gattaaatct gagataagca
     2221 aaagaatctt tttctctccc tctgagttta tcttttgcca atcgtcacca ggagcgtggt
     2281 agtcttcatg cactccagta aaaaaatgaa gcacagggat gtcgttagca taaaatacgg
     2341 tttggtcact tgtagcaaaa ccggagtcct tcgtttgtga tgttaaacca aggttagaat
     2401 taaaagattc aagaaggggt ttccactcag gagatgaacc agctccaaaa actgttagtt
     2461 tatcgtctct tagtctacca atcatatcca tattaatcat cgctagtgta ttacccaaag
     2521 gtattttagg atgtttaaca tagtaagaag aacctattaa ccccaattcc tcagctgaga
     2581 aggcaataaa aagaagactc cttttaagag aaaccctttg gtcggaaaaa tactcggcta
     2641 gttctagaag ccctgcaaca cctgaggcgt tatcatccgc accgttgtga accttattct
     2701 tatctgtttt atcgtctcct cttgatactc tgtcacccaa acctatgtgg tcataatgag
     2761 ctccgatgat tatcacctga tctttgagtg attgatcaga accctcgatg taacctataa
     2821 cgtttgcagt agggcttttt tcacggataa gttcggtatg aatctgggct tcagtttcag
     2881 gaata
//
LOCUS       DATDRL010000229         3210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2670191, whole genome shotgun sequence.
ACCESSION   DATDRL010000229 DATDRL010000000
VERSION     DATDRL010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2670191"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..435
                     /locus_tag="VI935_07825"
     CDS             220..435
                     /locus_tag="VI935_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HLE25543.1"
                     /translation="MLKGKVKWFNSSKGFGFIEQEGGGKDVFVHYSAIQGKGYKALSE
                     GEIVEFEVVEGPKGPQASKVVKLNNNK"
     gene            complement(542..1105)
                     /locus_tag="VI935_07830"
     CDS             complement(542..1105)
                     /locus_tag="VI935_07830"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HLE25544.1"
                     /translation="MIKKWNVVRSENVNSYKVFSTRKDISLSPMTGDEHDFYVVEAPD
                     WVNVVAITPDDQIVLIEQYRHGIRSITLEIPGGMVDDGESPVEAGKRELLEETGYTSD
                     VWVCIGKINPNPAIQNNTCYTFLASNARKVQDPSFEGTEDIAVLIKPTKNITNLVAEG
                     KITHALVVVAFYWFLLYDQHIRRIKGR"
     gene            complement(1258..1695)
                     /locus_tag="VI935_07835"
     CDS             complement(1258..1695)
                     /locus_tag="VI935_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HLE25545.1"
                     /translation="MAINMTPQEIKVFLAEQKVCRMATVDKSGMPHVIPMWYVILDKV
                     LFIETTGTTKKVRNVKSTKKASLVIDDGDSLHNFRGVMIQGNMVVVNDKAFFKKFREA
                     YAQRYFGSDEHPGYKFLTGIPNRVLLKFTPERFYSWDYRKLKF"
     gene            complement(2016..3023)
                     /locus_tag="VI935_07840"
     CDS             complement(2016..3023)
                     /locus_tag="VI935_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLE25546.1"
                     /translation="MITRILRGQAPDYVSNPKWINPRKNFVSAYIRTYETDKHILEDK
                     VEQALQVLQKCEVCPRNCHVNRLENKTAVCRTGRNAYVASAFPHFGEEDCLRGWRGSG
                     TIFFSFCNLKCVFCQNFDISWEGNGMILTPTELAKVMISLQEEGCHNINFVTPEHVVP
                     QIMEAIPLAIEMGLRLPIVYNTSAFDSSHSLEIMDGIVDIYMPDFKFWERKLSFFYMA
                     AEDYPDVVRAAIKEMHRQVGPLTFDENGLALRGVLVRHLVMPNRVASTREICRFIANE
                     VSRDTYINIMDQYRPEGRVLKLPQKYESINRPTVHAEYEEALKIAREEGLYRFDIRWM
                     H"
BASE COUNT          952 a          670 c          585 g         1003 t
ORIGIN      
        1 ttgactatcg gtgaatttct tgtgtagttg tgccataatc taaaagcctc ctttttggct
       61 ttagattacg acttcttagg gtgttcactt ttaatcttta aatcgtgtaa aaacggttca
      121 cttttaattt ttaaatgaca tgcagaatac ggtatgttga caagtctttt aaactgtgag
      181 atattaagag cagttttaag ctttaggagg taatttgatt tgttaaaggg taaagtaaaa
      241 tggttcaata gctcaaaagg ctttggattt attgaacagg aaggtggagg aaaggatgta
      301 tttgtccact atagtgccat tcaaggcaag ggctacaagg cactttctga gggtgagatt
      361 gttgaatttg aggttgtcga aggtcctaaa ggccctcaag cgagcaaggt agtcaaacta
      421 aataataata aatagctatc tattcccgct tcgctcccaa gtcaatttaa tattatcata
      481 taagcttggg taatggctta gcgattctat ataaggatga ggaggagtta tattgactcc
      541 gttacctgcc cttaatcctt ctaatatgct ggtcatatag aaggaaccaa taaaacgcca
      601 ctacgaccag ggcatgtgtt attttgcctt cggctactaa atttgtaata tttttagtag
      661 gctttatcaa cactgctata tcctccgtgc cttcaaagct cggatcctga accttccttg
      721 cattgcttgc aagaaatgta tagcaggtgt tgttctgtat agctgggttt ggattaatct
      781 ttccaatgca aacccacacg tcggaagtat atccagtttc ctcaagcagt tcacgttttc
      841 cagcttcaac tggagattcg ccatcgtcaa ccatgccacc tggtatctct agggttatag
      901 atctaattcc atgacggtac tgctcaatca atactatttg atcatcaggt gtaatagcaa
      961 ccacatttac ccaatcgggc gcctcgacaa catagaaatc gtgttcatct ccagtcatcg
     1021 gagaaagact tatgtccttc cttgttgaaa aaaccttgta tgaatttaca ttttcagacc
     1081 taacgacgtt ccactttttt atcattcttt atactcctaa atctaatccc gactctaaaa
     1141 acaacaatat atactaagct gtatcgtttg cgatacctat tggagtctaa aactttcatt
     1201 acatgagaga tagtggtcta tattggagtg aactaattag gagcttaata ggactgatta
     1261 aaacttcaat ttcctataat cccatgaata aaacctctct ggggtgaatt tcagaagaac
     1321 gcggtttgga ataccggtta aaaatttata acccggatgc tcatctgagc cgaaatatct
     1381 ctgtgcatac gcttctctaa actttttaaa aaaggcttta tcattaacta ctaccatgtt
     1441 tccctgaatc ataacacccc tgaaattatg taaagaatcg ccatcatcaa taaccaggga
     1501 ggccttttta gttgatttta cattcctgac cttctttgtc gttcctgttg tttcaataaa
     1561 gagaactttg tctaaaatta cataccacat aggaataaca tgtggcatcc cagatttatc
     1621 aacagttgcc attcggcata ccttttgctc ggccaggaag acctttattt cctgaggagt
     1681 catatttatt gccatgattt cacccctaat ttaagagaat tttctgtata gttgagttta
     1741 ctggctttat tcagatcaat caatcaaaac cacggaacaa tcttctacgc tgatgctttt
     1801 aggctctgtc atacctgaat gcttttttcg gttattcata agagttcatt tgctggattc
     1861 tagctttcgc ggcaatgaca aaggcataat ggattcctac ttaaggcgta ccaaataccc
     1921 ttatcaggta atttacagga atgacaacgt taatcatcgt tcgacacatt ccgtttcacg
     1981 cctgtgagtc cttaacctct attaattaat ggaaactaat gcatccagcg aatatcgaaa
     2041 cgatacagcc cttcctcacg tgctatcttc aaagcctcct catattccgc atggacagta
     2101 ggccgattaa tactttcata tttttgtgga agctttaaaa ccctaccctc cggtctatat
     2161 tgatccatga tatttatgta tgtgtctcta gatacttcat ttgcaataaa gcggcaaatc
     2221 tctcgtgtgc tagcaactcg gtttggcatt accaagtgac gaacgagaac cccgcgcagg
     2281 gctaggccat tttcgtcaaa tgtaaggggc cctacctgac gatgcatctc ctttattgca
     2341 gcacgcacta catccggata atcctcagca gccatataaa agaacgagag ttttctctcc
     2401 cagaacttaa aatcgggcat atatatatca acgataccat ccataatctc aagagaatgt
     2461 gacgaatcaa aagcacttgt gttatataca attggcaacc ttaaacccat ctcgatagcc
     2521 aatggaattg cctccataat ttgaggaaca acatgctctg gagtaacaaa atttatgttg
     2581 tggcagcctt cctcttgaag tgatatcata acctttgcaa gttccgttgg tgttagaatc
     2641 atcccattac cttcccaaga tatatcgaag ttctggcaga aaacacattt tagattgcaa
     2701 aaagagaaaa aaatcgtccc agaacctcgc caccctctca gacaatcttc ttcgccaaaa
     2761 tgcggaaatg cgcttgcaac ataggcatta cggccggttc tacatacagc ggttttattc
     2821 tcaagtcggt tgacatgaca attacgcgga cacacctcac acttttggag tacttgaagg
     2881 gcttgttcta ccttatcttc taatatatgt ttgtcggttt cgtatgtacg tatataagcg
     2941 gatacaaagt tcttacgagg atttatccat ttagggtttg aaacataatc tggtgcttga
     3001 cctcttaata ttcttgttat cattggagta tattaaaatt ataatatttt actagacgaa
     3061 tctcaaccca acgaattgcc tcaccagaaa tctatctgaa agaggatcgt tgcctcaaat
     3121 aagaatctat ttgaaatagg tcaaaatagt ctccgacaaa ttgtgcaata cggaggctga
     3181 agtgaggcta acgatgaaag agaggcaatc
//
LOCUS       DATDRL010000230         3279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1347771, whole genome shotgun sequence.
ACCESSION   DATDRL010000230 DATDRL010000000
VERSION     DATDRL010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347771"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VI935_07845"
     CDS             complement(<1..413)
                     /locus_tag="VI935_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE25547.1"
                     /translation="MKVSFLSGYPFEEYRYGVSEHIFQLSKALSLSLKVDSCHVISMY
                     GNTRSWKVLPKVWSHTIKREISHYLFPWSASTEIDRLVHEINPDLIHLHCATLPYTWA
                     AQKLSKQFPVVVTIHSDVAEEALYKPFKSKLWDKT"
     gene            complement(446..2329)
                     /locus_tag="VI935_07850"
     CDS             complement(446..2329)
                     /locus_tag="VI935_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25548.1"
                     /translation="MKIRSLLRFDIIAAILGVGVGAGAIFLSDLYNLNRSHLGFAVLI
                     AGFSYLFLNKYITSESRTKNLSYLQTSSRIFTSGSGLFFLLLIISIFVVRESLYNRSA
                     LYLGFFVSALIVLLIQITLLDCDPSWKQRVLLAQILLMTISFKAGAFYLYPSISGIDP
                     FYHQRYIDGLIATGFIQENAYSRFPSMQLLSGVFSLVVDTPSKNGFFYISILYSIALL
                     AVFPIGKKILNARAGLLAAMLVCIIDYQVFWGIQITPMTLAITFFLLVLMSLIGRRDS
                     QKSNVKLGWTIIIILISGAMILTHTLSTLIFFIALIVTLILSEYIKLTKRQSRKREMA
                     NWALVLLVGVAILSYWMNSFTMPGGSFFESVIFSIKSALSMAELGKVQMVSMAGSLDK
                     WEVFAGEAGWTLLLVPALIGAMVSLKFGAKKIEELTLVFVFATYLLVAYGAGITGVAG
                     ILPDRWISFLSILTSLLASLTLSRLLNKRKIVLSILILLFLFTTSFLLITSPTRVIPD
                     SPLYAQNLSTRPGFFASELSGMDYARDLSVIDLSASSLSKLYLSEAKEIDPRDPDTYQ
                     SQNLMVLREFDLNKGFFNPFPQDKINEFVPPPQEFLDYIHGLERIKVYDNGEVSLYLS
                     SNK"
     gene            complement(2326..>3279)
                     /locus_tag="VI935_07855"
     CDS             complement(2326..>3279)
                     /locus_tag="VI935_07855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLE25549.1"
                     /translation="PIYNENSMEYVLLTPARNEAVLLPFAIRSLLSQNHLPALWLIVD
                     DGSSDNTMQIIHELESSHSWIISKSLPGGLGGLGKHFAEVVDYGFKQLQLEAVARGIE
                     YDYIGKIDADVSFDPTCFSSLINEFEKDPSLGITSPRLIYCEPGEKGFYIHKEPDRSL
                     SDHPDDPIRLYRRKCFEDIGGIKIVRASETVAEAKAKLRGWKIYRFDNVYGKHLRKAH
                     SSTPLWSRLTLYGSEAYYLGYHPLLALGRLCFDIFFGRPRYRFLAYACGYIRGYIKRE
                     PRIDDDEILRYFWSQRLREIVSQLILRPEVVLFPRSKKRSS"
BASE COUNT         1097 a          657 c          614 g          911 t
ORIGIN      
        1 attgttttat cccagagttt tgatttgaat ggtttatata aagcttcttc agccacgtca
       61 ctatgaatcg ttactacaac gggaaattgt tttgacaatt tttgagcagc ccatgtataa
      121 gggagggttg cacaatgaag gtgaattaaa tcgggattga tctcatgcac caatcggtcg
      181 atttcagttg aagctgacca aggaaataag taatgggaga tttctctctt aattgtatga
      241 gaccaaacct ttggaagtac tttccaggat cgcgtatttc catacattga aataacatgg
      301 caagagtcga cttttagaga aaggcttaaa gccttagaca actgaaaaat atgctccgag
      361 accccatagc ggtattcctc aaatggatag ccactcagga aagatacttt catgaattaa
      421 ttcttgtaaa aactttgtac taaccctatt tattggatga caagtacagc gatacttcgc
      481 cattgtcata aacttttatt cgttccagcc catggatata atcaaggaat tcctgaggag
      541 gaggaacaaa ctcatttatc ttgtcttgcg ggaatggatt aaaaaatcct ttgttcagat
      601 cgaattccct tagaaccatt aaattctgag actgataagt atcaggatca cgaggatcga
      661 tttctttggc ttcactcaga tacaatttag ataaagaact tgcagataaa tcaataacag
      721 acaagtctcg ggcataatcc atacccgaga gttcagaggc aaaaaaacca ggtcgcgtgc
      781 tgaggttctg ggcgtaaagt gggctgtctg gaatcactct cgttggggag gtgatcaata
      841 agaaacttgt cgtaaaaaga aataacagta ttaatataga taaaactatc ttccttttgt
      901 ttagaagcct tgataaagtc aaggaagcca ataaggaagt tagaattgag agaaatgaaa
      961 tccaacgatc aggtaaaatt cccgcaactc cagttatccc ggctccataa gccactaata
     1021 aatatgtggc aaaaacaaag actaatgtaa gctcttcaat tttctttgct ccgaacttta
     1081 acgaaaccat tgctcctatc aaagcaggta ccaataacaa agtccaaccg gcttcaccag
     1141 caaatacttc ccatttatcc aatgaaccgg ccatagatac catctgaact tttccgagtt
     1201 cggccatcga aagggcagat ttaatagaaa aaatcaccga ttcaaaaaaa cttcctccgg
     1261 gcatggtaaa actattcatc caataggaca aaatagcaac tccaaccaga aggacaagag
     1321 cccaatttgc catttccctt tttctgcttt gtctttttgt caatttaatg tattctgaca
     1381 atatcaatgt gacgattaga gcgataaaaa aaattaacgt agaaagcgta tgagttaata
     1441 tcatcgctcc acttataagg ataataatga tagtccaacc aagtttcaca ttggatttct
     1501 gactatctct tcgtcctatg aggctcatca agaccagtaa aaagaaggta atagccaaag
     1561 tcattggagt aatttgaatc ccccaaaaaa cttggtaatc aatgatacat accaacattg
     1621 ccgcaagcag tccagcccga gcattcagga ttttctttcc tatagggaat acagccaaga
     1681 gagctatgga ataaaggata ctgatataaa aaaaaccatt cttggaagga gtatcaacaa
     1741 ccaagctaaa aactcctgat agcaattgca tagatggaaa tctactatat gcattttcct
     1801 ggataaagcc ggttgctatt aacccatcaa tgtaacgttg gtgataaaaa ggatcaattc
     1861 ccgaaatact tggataaaga tagaaggctc cagctttgaa tgaaatcgtc attaacagaa
     1921 tctgtgcaag taacacccgt tgtttccatg acggatcaca atccagcaat gtgatttgga
     1981 ttaataaaac aattaatgca cttacaaaaa accctagata taaagcagag cgattatata
     2041 gagattccct aacaacaaat atactaataa tcaataaaag aaaaaataat cctgatcctg
     2101 aagtgaatat tcttgaagat gtctgcaaat atgatagatt cttggttcta ctttcagatg
     2161 tgatgtattt atttagaaat aaatatgaga aacctgcgat aaggacagca aagccaagat
     2221 gagaccgatt taaattgtag agatcactta gaaaaatagc tcccgcgcct actccaactc
     2281 ctaggatagc cgcgattatg tcgaatcgta ataggcttct aatcttcatg acgaacgttt
     2341 tttgcttctt ggaaataaaa caacttccgg ccttaatatc aattgggata caatttcccg
     2401 taagcgttga gaccagaaat atcttaagat ctcatcgtca tcgatcctag gttctcgttt
     2461 tatataccct cgtatgtatc cacaggcata ggcaagaaaa cgatagcgtg ggcgcccaaa
     2521 aaagatatca aaacacaagc gacccaatgc aaggagagga tgataaccaa gataataggc
     2581 ttctgatccg tacagtgtca atcttgacca gagtggtgtt gaactatgtg ctttacgtaa
     2641 atgttttccg tagacgttgt caaaacgata tattttccac cctctgagtt tagctttagc
     2701 ctcagccacc gtttcagagg cacgtacaat ttttataccc ccaatatctt caaaacactt
     2761 acgccggtat aaacgaatcg gatcatccgg gtgatctgat agtgatcgat caggctcctt
     2821 gtgaatgtaa aatccctttt cccctggttc acaataaatt aaacgtgggc ttgttatgcc
     2881 aagcgaaggg tctttttcaa attcattgat taatgaggaa aagcaggttg gatcaaagga
     2941 gacatctgca tcaattttcc caatataatc atactctatc cctctagcta ctgcttccaa
     3001 ttgaagttgt ttaaagccat agtccaccac ctctgcaaaa tgttttccta atccaccaag
     3061 cccacctgga agcgatttgc ttatgatcca tgaatgggaa ctctcaagtt catggatgat
     3121 ttgcatcgtg ttatcagaac tcccatcgtc gacgatcagc cagagagctg gaaggtggtt
     3181 ttgacttaag agagatcgta ttgcgaaagg caatagaacc gcttcatttc gggcaggggt
     3241 taatagaaca tattccatgg aattctcatt gtaaatcgg
//
LOCUS       DATDRL010000231        11435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1081287, whole genome shotgun sequence.
ACCESSION   DATDRL010000231 DATDRL010000000
VERSION     DATDRL010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081287"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VI935_07860"
     CDS             complement(<1..257)
                     /locus_tag="VI935_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25550.1"
                     /translation="MDLQRKGVRPLFSVPFSPRRQILLGDDTFVASLEPYIKRVREVE
                     EVPRGQRFVDRPGLEELLRDVEYQPNAVRDNLIREAHWKHW"
     gene            complement(1259..1480)
                     /locus_tag="VI935_07865"
     CDS             complement(1259..1480)
                     /locus_tag="VI935_07865"
                     /inference="COORDINATES: protein motif:HMM:NF026928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm27 family immunity protein"
                     /protein_id="HLE25551.1"
                     /translation="MKCKCRDVNELWDEEAKQYADGHLEEVEVRAEGWEVLYRCPVTG
                     RKWLEDYPRSEEHGGGPLRLRQLTECEDG"
     gene            complement(1477..9756)
                     /locus_tag="VI935_07870"
     CDS             complement(1477..9756)
                     /locus_tag="VI935_07870"
                     /inference="COORDINATES: protein motif:HMM:NF026858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 10 domain-containing
                     protein"
                     /protein_id="HLE25552.1"
                     /translation="MIERFLKKRQLLPSLFLLFITFIKPTELLAVGEYYDETGFRSNK
                     TYISYSPQEYINPFSGNLILSHTDITLPGNGGLDLTIQRTYNSKIFSEITSSTFGIAG
                     GVLGSLGVGWDLHFGRISDLGLLDNVTLINANPFFLQMPDGSIHSILNNDHQNINSNV
                     NSQFITEDFWLINYDGVNNRYIMTLPDGTVYTFGQHVTDSLSYSFYYVTSIKDKNGNE
                     ISIEYFDCCNMSDPNPDSGNIGTCTNSNPLTKTRHIKGVIDSVGRVINFSMLNNSCRG
                     NAISSIDVNGSVYNYIYFPGTDGFPFTEPIPFYDGGSGRLLKEVKPPVGPSWQYFYDV
                     DTNDGEPDGELISVTYPSGGTVQYDYETFALNLLQDSANQPKPFNFRATVSRTRSGPN
                     ITPGTWALSYAPASLQDTTIVTDSCGTKEVYKFFGLREGSFNGVWNIGVLYNKETKDI
                     NDNLFELEAKSWVPYQISNNGLDPITLINGAFLPLLNSRTINRDGISYTTDFTYNRFY
                     DSPTQIVENGELSRTTDITYFEDLTPGNYIIGKPLVTTFTSGTQTKTITNSYDSKGNL
                     ISVDKYGVTTVFSYHPDGNLAWEKNARGFYTHFDQYNFGVAGVIKYGSSAESADDSVY
                     TEARTINWEGTIASLTDGRGNQTLFNYDGINRITSVTPPPSGEAQTLITYGNVSGRDY
                     IIKKGISEVKYSLNGFGKPIGAQSNVGVDTEIRYDECGRRIYESLPFAFDNSTPNTGD
                     SFTYDALERVKRITHPDNTSISYSYSGNTVTINNERNLATTYNFKSFGDPEDKRLSSI
                     KDQQNNTTSYEHDLLGNITRVDLPIGGDRVFTYNSKNFLASENNPENGTTTYLHDEIG
                     NVISKTNADSKTINYQYDSLNRLTFIDHPGGEDDVTYAYDDANNRTLMEGSSGGYSYS
                     YLYDRSNRLTRQNVILGGVSYGVDFVYDERNNLIQAIYPSGEIVNYNYDAGNRLLDVY
                     DSANILFVGNISYHPSGAPSHYINANSVSSDFTYDSNHRLKSLKISRPFPELVLVKEG
                     LGSGTVASSPLGINCGIDCRQVYPADGITITLTATPDPDSNFVGWSGDPDCADGVVLM
                     DANKTCVVTFALEPVQFTLTVNKTGTGGGTVTSSPGGIDCGSDCTEGYDVNVQVTLTP
                     APDAGSSFTGWGGDPDCSDGVVTMDASKTCTATFELATQQYTLGVVNNSDGFGSGTVS
                     SNPAGISCSSGTVGDCTEDYNENTTVTLTATPATGVFGSWGGDADCSDGVVTMNSDKT
                     CSATFVEFFTFTVLKSGTGSGKVTTLDGGINCGGDCTEVYGANNLLFLNATPDPGSKF
                     TAWGGSCSGGSAISLLLDSNKTCTATFDLLPGHTLMLEKQGNGTGTVTSNPTGINCGS
                     DCSENYFEGTQITLTPTPNAGSIFSGWSGDADCSDGVVMMNSSKTCTATFNLKPPQFT
                     LTVSKSGTGTGIVTSNPPGISCGSDCTEAYDINSQVSLTAAPNTGSSFTGWGGDADCL
                     DGLVTMDANKTCTANFTTNPQSTLTVTKSGTLTGTVTSNPAGIYCGIDCTQSYSLNTR
                     VTLTPTPDSGSIFGGWEGPADCSDGVVIMDASKTCTAIFLFEGEINQVFKEAQRLVAS
                     DGNALGGFGSGVTIDGDTAVMGAPLAKIGKKTEQGAAYVFERDAGGVWRQVKKLTASD
                     GAMDDNFGASVSASGDWAIVGIKVSNPNNPDSGKQGKAYVFKRDQNGVWSQKQILSAS
                     DGKLNDEFGEEVVISGSRLVVRAHTADVGSNIGQGAVYVFELDANGTWNQVTKLFSPV
                     PNQIPANPPDFFYGIDKVAFSGDRILTQYNSGGSPLYAYLDFFERNINGVWSHKQSIL
                     DVGFDWGNISGDTAIVDGKVFKRSSNGTWAQVQSIKGGDAYLSGDYAVIDQRGSDDSI
                     LYRMGSDGVWWSIQVINRQGQTDINGDRAVIQSTVYELVNTFNLTLSKSGDGTGMVTS
                     SPSGINCGTACLSSKSAFAENTQVTLTAVPNSDSIFEGWSGDAECSDGVVTMNATKTC
                     TATFTKAAQLYTLTVSVSGSTNARVTSNPPGISCPSNCTEDFAEKAQITLTPVPYGVS
                     EIFLGWSGDADCLDGIVTMDTNKFCIATFEESCACSDPKAIIGTSGNDTLRGTSKNDI
                     ICGLEGNDSLTGQGGDDCLYGGPGNDTLQGGQNDDTLRWDEGNDRLNGQSGTDNLDGG
                     TGTDTCLSGETNTTCESLSGLKEINPPANRSKFITKEESHLNSLENLNKISIKISSHT
                     GSRVSRISSYVESLRLILVSVLFASNAFAQIPPTAFIDLEYFYDGVSNVTGVIDHNDS
                     ANNCTMQYDSLDRLTNADGSWGAGSFTYDSVGNRMSKDIAGENVNYSYGADNRLSGVV
                     HDANGNIIDDGTFTYTYDSENRLVSVPNGTDVITYEYDGDGRRIKRVTSGETTYYAYG
                     VGLNVLTEFSGQRVPKFDYIYAGNRNIARVNFDATGTPESKTFYHNDHLGSNIAITDA
                     TSTVEWDRVYLPYGEGFNDPNVDYLQNTHEYTAKELDEDSGLYYYGARYYNPELGRFM
                     SVDPSDGDITEPQSWNRYAYVLNNPFRYVDPDGEAAVNVSGIVKLNTQLRNKLGFLPN
                     LLLDLLLPVEPEAIVASSIPGLQALGTGTISKGVGNAITKVVNNPVPKRVARVVDKRV
                     IDRGATRLGAPDAEDVFVTAADDVMGIQSSEALAERLTLLNKAGELRRGPFAVIEFDT
                     PSGIASPIFRTNPGFVGFGRTANGAREFVVPNLTLEELINAIIRTVP"
     gene            10361..11419
                     /locus_tag="VI935_07875"
     CDS             10361..11419
                     /locus_tag="VI935_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25553.1"
                     /translation="MRLIYEDEEIRIGNGFPSLVRFFQGNSTKPLVVFFPGWAHLGRI
                     SYSFPGCNKEHFIAQWINQKGYSFLATSYPLDHAVYDRVYPEFTLTDWGKMAAEITNQ
                     FILENALPKEVVGISWSAAGQVIRPFNVACKSLGINVLFHLGLEASPALLIPSDRTIG
                     MKKTEKGMVSLRDSHYDLFWKEIEEQSQLNGEEVIPKENYLMDFLGDIPIALIETNEC
                     FENGHFVTNIDKSLEDKNFFAFTEYPLIANISGDSKLAPYHPIVDNWTWGFLITRKVY
                     HDYFVKNQESLSKISEAKFKKLVEYVNTLPERLHSHISGNHFLFVGRYGAQKIADYVE
                     HFDKEIEKIEQELKTFLR"
BASE COUNT         3230 a         2603 c         2165 g         3437 t
ORIGIN      
        1 taccagtgct tccaatgagc ctcacgaatc aaattatctc ttactgcatt aggttgatac
       61 tccacatccc ttaatagctc ctctaaccca ggcctatcta caaatctctg tcctctcggc
      121 acctcttcca cttcccgtac cctcttaata taaggctcaa gacttgcgac aaaggtgtcg
      181 tcccccaaca gaatctgtct tcttggagaa aaggggacgg agaaaagggg acgcacccct
      241 tttctctgaa gatccaatgt ttttgtttcg attggacttc gtgaatatta cttgacaagt
      301 ataaaggaat tgttgtacta tcttaaccga attgagaggg cggggtgaag gcatggaggt
      361 aagaaaatgc cagtttcaat atgttgtttt ccttctgaaa gattctcaca tctatcagtt
      421 acaacctcat ctacaatcta catctcttct gttctcaaga tacattgcaa aataaagatt
      481 ctaaaatctg aatcaaggag gtgtaactat gaaaaccttt gttagagttt tcattattct
      541 aggtgcaata ggagcgatgg tctcagtcgc cctaccggct ctggcaggag tcgctccccc
      601 ggcggaagat gctctttgtc ttgcggattg cgcagatgcc ttcggtgaat gcaaaagtgc
      661 ttgcggtatg acctgcacgg atccacaatg tgtacctggt gaatgtagtg atatgtgcat
      721 caatgacctt gtggattgcg ttgctatctt ttgtgaattt gtcccccggc cgcggtgtgg
      781 cgacaataat atcgacccgg gggagcagtg cgacccgccc gggatgcttt gttccggcat
      841 ccgggtctgc aatattaact gcagatgcgt gcctgtacag gggggtgact gaaatctcat
      901 cgccctgcca gaacgcttag gcacttgtcc gtaatcagaa ggctatcaga cattctccgt
      961 caggtgagat attggtaagc ggatttgcaa tgatcacgat ttcccctatc agacggcgtg
     1021 ggttaaacta gcaatccgtc ttcagaaaac gagcggggtc aggtctcaac atttgacata
     1081 aacagataat gaatgttgaa agttgagacg cgaccccatt gactttgctt tcaacgaatc
     1141 ttaaaaagtg cagagtcaag aaacaacctc acaaattagt tagtgaaata aaaattcaca
     1201 attcaagaca cacccgaatt ctttattttt ccctcatttt tgcctttggg acgtgaactc
     1261 atccgtcttc acattccgtt agctggcgta atcgaagtgg gccaccgcca tgttcctcgc
     1321 tccgtgggta atcttcgagc cattttcgac cagttactgg gcaccgatat agaacttccc
     1381 atccctccgc acgcacctcg acttcttcga gatgcccatc tgcatattgc ttagcctcct
     1441 cgtcccaaag ttcatttacg tctctacatt tgcatttcat ggtacggtac gtataatagc
     1501 gtttatcaat tcctctaaag tcaggttcgg tacaacaaac tcccgagcac catttgcagt
     1561 tcgaccaaat ccgacgaagc caggattagt tctaaatatt ggtgaggcaa tgccactagg
     1621 tgtgtcgaat tctattactg caaacgggcc acggcggagc tcgcccgcct tattaagtag
     1681 ggttagcctt tcagccagag cctcggatga ctgaataccc ataacatcat cggcagccgt
     1741 aacaaaaaca tcctcggcgt cgggcgcacc gagtcgagtt gctccacgat ctataacgcg
     1801 tttatccact acccgcgcaa cgcgtttcgg tacaggatta tttactacct tagttatagc
     1861 gttgccaaca ccctttgata tagtccccgt cccaagcgct tgtagtccag gtatagaact
     1921 tgcaacgatt gcttccggtt ccaccggcaa tagcaaatca agtagcaaat tagggaggaa
     1981 tcctaatttg ttcctcaatt gcgtatttag ctttactatt cctgaaacat ttaccgcagc
     2041 ctccccatca gggtcaacgt atctaaaagg gttgtttaat acgtaagcat atctattcca
     2101 actctgtggt tcggttatat caccgtccga tggatcaacg gacataaacc ttccaagttc
     2161 tggattgtaa tatctcgctc catagtagta gagtccggag tcttcatcta attccttcgc
     2221 tgtgtattcg tgagtgttct gcagataatc gacattcgga tcattaaatc cctccccata
     2281 tggcaaatac accctatccc actccaccgt gcttgttgca tcggttatcg ctatattcga
     2341 ccctagatgg tcattgtgat agaacgtctt gctctcaggt gtcccggttg catcaaaatt
     2401 aacccttgct atattcctat ttcccgcata tatgtaatca aacttcggca ccctctgtcc
     2461 gctaaactct gtaagcacat ttagccccac cccataggca tagtaagtgg tttcaccact
     2521 cgtaaccctc tttatccttc ttccatcccc atcgtactca tacgttatta catctgttcc
     2581 atttggtaca ctcactaacc tgttctcaga atcataggtg tatgtaaatg ttccatcatc
     2641 tattatgttc ccatttgcat catgaactac accgctcaga cggttatctg ctccatatga
     2701 gtagttaaca ttctcccctg ctatgtcctt gctcatccta ttcccaactg aatcatacgt
     2761 gaacgaacct gcaccccaag atccatctgc attagttaat ctatcaagac tgtcatactg
     2821 catggtacag ttattggcag aatcattgtg gtctattacg cccgttacat tgctaacccc
     2881 gtcgtaaaag tattcaaggt ctatgaaagc tgtgggagga atctgagcaa aggcatttga
     2941 tgcaaaaagc acagaaacca agattaatct taaggattca acatacgatg atattcgact
     3001 gactcttgaa ccagtatgag aacttatttt aattgatatt ttgttaaggt tttccaaaga
     3061 attaagatga ctttcctctt tggtgatgaa cttacttcta tttgcgggag gattgatttc
     3121 ttttaaacca ctaagacttt cgcaggtagt gtttgtttcc ccacttaaac aagtatctgt
     3181 accagttcca ccatctaagt tgtccgtccc agactgacca ttaagcctat cgtttccttc
     3241 atcccatctt aaggtatcat cgttttgtcc accttgaagc gtatcgtttc ccggacctcc
     3301 atataaacaa tcatccccac cttgcccagt caagctatca ttaccttcga gaccgcatat
     3361 gatgtcgttt ttagatgttc cacggagggt atcatttcca ctggtgccaa taatcgcttt
     3421 gggatcgcta caagcacaac tctcctcaaa ggtagcgata caaaacttgt ttgtatccat
     3481 tgtaactatt ccatcaagac agtcggcgtc tccgctccac ccaagaaaaa tctcactaac
     3541 tccataaggc acaggtgtca gcgttatttg agctttttcg gcaaaatcct ctgtacaatt
     3601 tgatgggcaa gaaatgccag gcgggttact agtcactcgt gcatttgtag aacctgatac
     3661 actcactgtc aaagtgtaca actgcgcggc ttttgtaaag gtagcagtac aggttttagt
     3721 tgcgttcatt gtaacaacgc cgtctgaaca ttcagcatcc ccgctccacc cttcaaaaat
     3781 tgaatcagag tttggaacgg cggttagcgt tacttgagta ttctcggcaa aggcagattt
     3841 actactcaaa caagcggttc cgcagtttat tccagaaggg ctgctagtca ccatacccgt
     3901 tccgtcaccg gacttactta gagtgagatt aaatgtgttc actaattcat aaacggttga
     3961 ctgaataaca gccctatcac cgtttatatc tgtttgtcct tgccggttaa ttacctggat
     4021 gctccaccat acgccgtcgc tgcccattct ataaagaatc gaatcatcac taccacgttg
     4081 atcaataacc gcataatcac cgcttaggta tgcatctcca ccctttattg actggacttg
     4141 cgcccaagtg ccgttactag aacgcttaaa aaccttacca tcaactatag ctgtgtcccc
     4201 actaatattt ccccaatcaa acccaacatc taatattgac tgtttatgac tccaaacccc
     4261 attaatattt ctttcaaaga aatcgaggta agcgtataaa ggagagccgc cgctgttata
     4321 ttgtgtcaaa atcctgtccc cactaaaagc aaccttatca attccataga agaaatcggg
     4381 cggattggca ggaatttgat tgggaaccgg ggaaaataat tttgtaactt gattccatgt
     4441 tccatttgca tccagctcaa aaacatagac tgcaccctgc ccaatatttg atccaacatc
     4501 ggcagtatgt gctctaacaa caagcctgct tccacttata acgacctcct ctccaaactc
     4561 atcgttaagc tttccgtcgc ttgcactcag tatctgcttc tgactccata ccccattctg
     4621 gtctcgtttg aaaacatatg ccttaccctg ttttccacta tcaggattgt tgggattaga
     4681 gaccttaata ccgactatag cccagtctcc gcttgccgat acgcttgctc caaaattgtc
     4741 atccatagcg ccgtcgcttg cagttaattt tttcacctgc cgccacactc caccggcatc
     4801 gcgctcaaac acataagccg caccctgttc ggttttcttt ccaatcttcg ccaatggggc
     4861 tcccattacg gcagtatctc catctattgt gacaccgctt ccaaaccctc caagtgcatt
     4921 cccatcgctt gctactagtc tttgagcttc tttaaatacc tgattaattt ccccttcaaa
     4981 caaaaatatt gccgtacatg tcttactcgc atccattata accactccgt ctgaacaatc
     5041 cgcaggacct tcccatcctc caaagattga tccggaatcg ggtgttggcg ttaacgttac
     5101 ccgtgtattc agagaataac tttgcgtaca atcgattcca caataaatac cagcaggatt
     5161 gctggtaact gtacctgtta atgttccaga ttttgtcacc gtcaaagtag actgcgggtt
     5221 tgttgtgaaa ttagcggtac aggtcttatt tgcatccatt gtaaccaagc cgtctaaaca
     5281 atcagcatca ccaccccatc ctgtaaagct tgatccggta ttaggagcgg cggttaggct
     5341 tacctgcgag tttatatcat aagcctctgt acaatcacta ccacaactta ttccaggggg
     5401 attgcttgtt actattcctg taccagttcc agatttactc accgtgagtg taaactgtgg
     5461 gggttttaaa ttaaaagtag cagtacatgt cttacttgaa ttcatcataa ctacaccatc
     5521 tgaacaatct gcatccccgc tccatcctga aaagatcgaa cctgcattag gggttggagt
     5581 tagtgttatt tgtgtgccct cgaaataatt ctcagaacaa tcgctgccac aattaattcc
     5641 agttggatta ctagtgaccg ttccagtgcc attgccctgt ttttctagca taagtgtgtg
     5701 cccgggcaat agatcgaatg tggcagtaca ggttttattt gaatccaata ataaagatat
     5761 agccgaccca ccagagcaac ttcctcccca tgctgtaaat ttagagcccg gatcgggagt
     5821 tgcatttaag aataagagat tatttgcccc ataaacctcc gtgcagtcac cgccacaatt
     5881 tattccaccg tcgagggttg taacctttcc gcttcctgta ccgctcttta gtactgtaaa
     5941 tgtaaaaaat tctacaaatg tggcactgca tgtcttatca gaattcatcg taaccacacc
     6001 atccgagcaa tccgcatctc caccccatga tccaaatacc ccagtagcag gagtcgctgt
     6061 aagggtcact gttgtattct cgttgtaatc ttcagtacaa tcaccaacag taccgctact
     6121 acaggaaatt ccagccggat tactagaaac cgttccagat ccaaatccat cactattatt
     6181 tacaactcca agcgtatatt gttgagtagc cagttcaaac gtagcagtac atgtcttgct
     6241 tgcatccata gtaaccacgc cgtctgaaca gtcaggatcg ccgccccaac ctgtaaatga
     6301 tgaacccgcg tcaggtgccg gtgtcagcgt tacctgaaca ttcacgtcgt aaccctcagt
     6361 gcagtcgctt ccacaatcta tcccccccgg gctgcttgtt actgttcctc cccctgtgcc
     6421 ggttttgttt actgtgagcg taaactgcac tggttccaaa gcaaaggtta caacacaggt
     6481 cttgttcgca tccataagca ctactccgtc tgcgcaatca gggtccccgc tccagcccac
     6541 gaagttagaa tcaggatcgg gagtagcggt cagggttatc gtaatgccgt ctgcaggata
     6601 aacctgtctg caatcaatac cgcaatttat tccaagagga ctgcttgcta cagtaccact
     6661 gcctaaaccc tcttttacca atactaactc aggaaaaggc cttgaaatct taagggattt
     6721 taagcggtga ttactatcat aagtaaagtc tgagctaacg ctattggcat ttatataatg
     6781 ggaaggagcc cctgaaggat ggtaggaaat gttgcctaca aataaaatgt tcgccgaatc
     6841 atatacatct aataatctgt ttcctgcatc ataattgtaa ttaactatct cccctgaagg
     6901 atatattgct tgaataaggt tatttctttc atcataaaca aaatcaacgc cataacttac
     6961 accacctaaa attacattct gtctagtcaa cctgttcgat cggtcgtaca gataagaata
     7021 cgaataacct cctgaactgc cttccatcaa agttctattg tttgcgtcat cataggcata
     7081 agtaacatca tcctctcctc caggatgatc aataaatgtc agacgattaa gtgaatcata
     7141 ttgataattg attgttttac tgtcggcatt tgtttttgat ataacatttc caatttcatc
     7201 atgtaggtaa gttgtagttc cgttttcagg gttattttct gaagccagga agtttttaga
     7261 attatatgtg aagactcgat caccacctat cggcaaatcc acacgggtaa tatttccaag
     7321 aagatcgtgt tcataagaag ttgtattgtt ttgttggtct ttaatgctag agagtctttt
     7381 atcttcagga tcgccaaaag acttaaaatt gtaagtggtg gctaggtttc tttcattatt
     7441 aatcgtaacg gtattgcctg aataagaata gcttatagaa gtgttgtcgg gatgtgtaat
     7501 cctttttact ctttcaagcg catcataagt aaaactatcg cctgtattcg gggttgagtt
     7561 atcaaaggca aagggaagcg attcatatat cctgcggcca cactcatcat atcttatttc
     7621 ggtatcgaca ccgacatttg attgagcgcc aataggttta ccaaagccat ttaaactata
     7681 tttgacttcg gaaatacctt ttttaataat gtaatcccga ccgcttacgt taccataagt
     7741 aattaaggtt tgagcctctc cactgggtgg aggagttact gaagttatcc tgtttatgcc
     7801 gtcgtaatta aataaagtct gattgcccct tccatcagtt aagcttgcaa tcgtcccttc
     7861 ccaattgatt gttcttgcct cagtataaac agaatcatcg gctgattcgg ccgacgaacc
     7921 atatttgata actcctgcaa ctccaaaatt atattgatca aaatgggtgt aaaaacctct
     7981 tgcattcttt tcccacgcta aatttccatc tggatgataa gaaaacactg tagttacccc
     8041 atatttatct actgaaatta ggtttccttt gctgtcataa gagtttgtta ttgttttggt
     8101 ttgagtgccg gatgtaaaag tagtaactaa aggcttcccg ataatataat tacctggtgt
     8161 aagatcttca aaataggtta tatctgtagt tctagataac tcaccgtttt ctactatttg
     8221 ggtgggtgaa tcataaaacc tgttataggt aaaatctgtt gtataactaa taccgtccct
     8281 atttatcgtc cttgaattca agagtggcaa aaatgcccca tttataaggg taataggatc
     8341 taaaccatta ttagaaatct gatatgggac ccaactttta gcctctaact caaatagatt
     8401 atcattaata tcctttgttt ctttattata tagcactccg atgttccata caccgttaaa
     8461 agaaccttct cttaatccaa agaatttata tacttctttt gtaccgcaag agtctgttac
     8521 tatggtagta tcttgtaaac tagccggagc ataagagagt gcccaagttc ctggagttat
     8581 atttggtcca gatcgggttc tagatactgt tgctcgaaaa ttaaatggct taggttggtt
     8641 cgccgaatct tgtaataggt ttaatgcgaa tgtttcataa tcgtattgaa ctgtacctcc
     8701 tgatggatac gtaactgata ttaactcccc atcaggctcc ccgtcattcg tatctacatc
     8761 atagaaatat tgccagctag gtcctactgg aggttttacc tcctttaata gacgacccga
     8821 tcccccatca taaaagggta taggttcagt aaaaggaaaa ccgtcggtac caggaaaata
     8881 aatgtaatta tagacacttc catttacgtc aatcgaacta attgcattac cccgacatga
     8941 gttattaagc atactaaaat ttataactct tcctacgctg tcaataaccc ctttaatgtg
     9001 ccgtgttttg gtaagaggat tggaattggt acatgtgcct atattgccgg agtcagggtt
     9061 cggatcactc atattacaac aatcaaaata ttcaatactt atctcatttc catttttgtc
     9121 ctttattgaa gtaacataat agaagctata ggaaagagaa tcggtaacgt gttgaccaaa
     9181 ggtataaaca gttccatcgg gaagagtcat aatatatcgg ttatttacac cgtcatagtt
     9241 tattaaccaa aaatcctctg taataaattg actgtttaca tttgaattta tgttctgatg
     9301 atcattgttt aatatagaat gaattgatcc atcgggcatc tgtagaaaaa agggattagc
     9361 attaattaaa gtaacgttgt ctaacaatcc taaatcactt atccttccaa aatgtaaatc
     9421 ccagccaacg cctaaacttc ccaaaactcc accagcaatg ccaaaggtac ttgaggttat
     9481 ttctgagaaa attttactgt tatacgtcct ttgtatggtt aggtctaatc ctccattccc
     9541 aggcaaagtt atgtccgtat gagaaagtat aaggtttccg gaaaaaggat taatatattc
     9601 ttggggtgaa taacttatgt atgttttatt cgacctgaac cctgtttcat catagtattc
     9661 tcctacagca agtaactcgg ttggcttaat aaaagtaata aataataaaa acaagctagg
     9721 taataactgc ctttttttaa gaaagcgttc tatcattttt aattttcatg cttgcaagtt
     9781 ttggaatttc acgcgctaaa gtataaatgc catttctaaa aaaagattaa aaaatttttt
     9841 cttgacaaaa tttccaacaa agtcaatatc attaaaatca gatcaccact taaattggtt
     9901 tataaaacag cgaaatgagt taataagtga gcaaggtcag ttattgatct tatgccgacc
     9961 taggagttct agcagttttg attcacccct tggaggaaaa gctgtcagat taagtcgcct
    10021 tttttgacga aagtttatta atatataata atagggcttg agaatgaaga ttgactttta
    10081 tcaaccaagg tgatttgtaa ctatcaaggc tttgcctacc aaacttggaa catcgaaaaa
    10141 tatttccctg atggttgggc tattaagacc ggtaacttct acgggaaggt gatcgaaccc
    10201 acgccagact attcgtagat tgccacggca ttcggagggt acggtgaacg tacaagcact
    10261 ctcaaaacgt tggagtcagc tctacgccga gagcttgaag ctgtggagag gggaaatgtt
    10321 gcagtcattg atgtgattct ggaaccgtag agattaacaa atgcgactga tttatgaaga
    10381 tgaagagata cgaattggca atggatttcc ttctctagtt agattctttc aaggaaactc
    10441 tacaaaaccc cttgttgttt tctttcctgg atgggctcac ttaggaagga ttagctatag
    10501 ctttccaggg tgcaataaag agcactttat agcacaatgg atcaatcaaa aggggtattc
    10561 ttttttggcc acatcttatc cattagacca tgctgtctat gatcgtgttt atccagagtt
    10621 tactttaaca gattggggaa aaatggctgc ggaaattacc aatcagttca ttttagagaa
    10681 tgcgctccca aaagaggttg tagggattag ctggagcgct gcaggacagg tgataagacc
    10741 ctttaatgtt gcctgcaaat cattagggat caatgtgctt tttcatctag ggttagaggc
    10801 atctcccgct ttacttatac cttctgatcg gacaattgga atgaaaaaga cagaaaaagg
    10861 catggtatct cttagagatt ctcactacga tctgttttgg aaagaaatag aggaacaaag
    10921 tcaattaaat ggagaggaag tcattcctaa agagaattac ctcatggact ttttagggga
    10981 tatccctatt gccttaatcg aaactaatga gtgctttgag aatgggcatt ttgttacaaa
    11041 tattgataag agccttgaag ataaaaactt ctttgcattc accgagtatc ccctgattgc
    11101 aaatatttcc ggagattcta aactagcgcc atatcatcct atcgttgata attggacttg
    11161 gggttttttg ataactcgga aggtttatca tgattatttc gtcaaaaatc aggaatctct
    11221 atctaaaatt tcagaggcaa agtttaagaa attagttgaa tacgtaaata ccttgcctga
    11281 acgtttgcat agccatattt caggaaacca tttcttgttt gtaggaagat acggcgccca
    11341 aaaaatagca gattatgtgg aacattttga taaggagata gaaaaaatag agcaggagct
    11401 caaaaccttc ttgagatgaa tccaccacaa tgtaa
//
LOCUS       DATDRL010000232         4506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3827760, whole genome shotgun sequence.
ACCESSION   DATDRL010000232 DATDRL010000000
VERSION     DATDRL010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3827760"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /gene="purF"
                     /locus_tag="VI935_07880"
     CDS             <1..1080
                     /gene="purF"
                     /locus_tag="VI935_07880"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HLE25554.1"
                     /translation="NALTLKEELEQSGAIFQSTTDTEVIVHLIARSREQSLIKRIIEA
                     LMRCRGAYSLLILSPEFMIATRDPFGFRPLVLGRLEDAVVVASETCAFDLIEAEFVRE
                     LEPGEILVVSPRGVESFKPFPEVGHKYCVFEFIYFARPDSFMFGRNVYQVRKELGKQL
                     AREQPADADIVVAVPDSGVPAAIGYSEESGLPLELGLLRNHYVGRTFIEPQQSIRNFG
                     VKLKLNAIREVLSGKRVVIVDDSIVRGTTSRKIVKMIRAGGAKEVHMRISSPPMCFSC
                     YYGVDTPIREELIASSLDAEEINKYITSDTLGYLSLEGVMKAVESYKGLDGNEGFCDA
                     CFTGNYPVEITDSKVQLKQLQLFDR"
     gene            complement(1124..2155)
                     /locus_tag="VI935_07885"
     CDS             complement(1124..2155)
                     /locus_tag="VI935_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HLE25555.1"
                     /translation="MLEISSRRFNYKKLSLVVVGLLFALILCEIILRLFVPVGIIGHT
                     ITIYDPIYGSRLKKSYTAKWITSEFIIQYTTNSLGFRGPEPKTFPYRPILFLGDSYTE
                     GYGVSDGEEFPELIRKQLAEQFGETRISVVNAGIENTGNAHWVKFLKNEVKRFNPRLV
                     VLQFMDNDFNDNVKEQLFTLSPSDSLVELPVPPPSFERRIQKVIEFIPFIANSYILGL
                     GRHIFYLLASYRNTPKDIVNVSSGIDNGFEERLTFRLWQEVLEICKQNNWPVLAIIVY
                     INGNRLTKLENLLKRYNVESLRVPEYEDRPDLYYKVDGHWNALGHAYVAELVVKSLTV
                     SNKYRYLFK"
     gene            complement(2456..3403)
                     /locus_tag="VI935_07890"
     CDS             complement(2456..3403)
                     /locus_tag="VI935_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLE25556.1"
                     /translation="MTTIQLKEADKVEILTILDNSLDLLLPSTDQVKRVPLTPDALTR
                     ETLVAEHGFAALVTVISGNTSESLLFDAGLSKNGLIHNMDVLDVNPEEFHAIVLSHGH
                     TDHTQGLVGMIKRLGKRKMPLLLHPDAFLKCKVVFPDGHELGLPPPDRGILTQEGIEI
                     IEERGPSYLLGGLVLVTGQIHRSTPFEQGFSIHYAYIGNKWQQDPLIHDDQAVVLNVK
                     GRGLVVVTGCGHAGAINTIRHAQELSGIQKVYAVREGFHLSGRLFEPIIPPTVAALKA
                     LNPAIIVPAHCTGWKAAHAIARELPEAFVPNTVGTRFVL"
     gene            complement(3625..4017)
                     /locus_tag="VI935_07895"
     CDS             complement(3625..4017)
                     /locus_tag="VI935_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25557.1"
                     /translation="MGARHVVEAYHNSWTKGKPAEARSYLDNDLHFQGSIDKFRRADD
                     FVKTRTQIQRMLKGVTPPRNFFSENGSVLLYDCDTMSPAGVIRAAEFFKVENGEINEA
                     RLFFNGNELRKLMGSRYPKFVLLSLKMA"
     gene            4299..>4506
                     /locus_tag="VI935_07900"
     CDS             4299..>4506
                     /locus_tag="VI935_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25558.1"
                     /translation="MSILNFIIKDFNYTILSAISLLSTLIISISCAKVEVYPVQNQYG
                     SKGKILTASGPGVRFYRPALYVWIT"
BASE COUNT         1358 a          952 c          877 g         1319 t
ORIGIN      
        1 aacgcattaa cacttaaaga ggaactcgag caaagcgggg cgatctttca atctacaact
       61 gatacagaag taatcgtaca tctaattgct agatcaaggg agcagagtct tataaagagg
      121 ataatcgaag cccttatgag atgtaggggc gcttattctc tcctaattct ctcccccgaa
      181 tttatgatcg caacacggga tccctttgga tttagacccc ttgtactagg aagacttgaa
      241 gatgcagtag tggtcgcttc tgaaacatgt gcatttgatt tgattgaggc agagtttgta
      301 agagaactag agcctggcga aatattagtt gtaagcccga ggggcgtcga atcctttaaa
      361 ccatttcctg aagtaggaca taaatattgc gtttttgaat ttatctactt tgcaagacca
      421 gatagcttta tgtttggaag gaatgtttat caggtaagaa aagagctcgg aaagcagctt
      481 gcgcgcgagc aacccgctga tgcagacatc gtagtagcag ttcctgactc aggagttcca
      541 gcagcgatag ggtattctga ggaatctggg cttccgcttg agctagggct tttaagaaac
      601 cattacgttg gaagaacatt tatagaaccc cagcaatcca taagaaactt tggagtaaag
      661 ctaaagttaa atgcgataag agaagtgctt tccggaaagc gagtagtaat cgttgatgat
      721 tcaatcgtca gaggaacaac aagcagaaag atcgtcaaga tgattcgcgc cggaggggca
      781 aaagaggtgc atatgagaat cagttcacct cctatgtgct tttcatgcta ctacggagta
      841 gacaccccga ttagagagga actcattgcc tcctcattag atgccgaaga gataaataaa
      901 tacataacct ctgatacact tggatacctg agtcttgagg gcgttatgaa ggccgttgaa
      961 agttataagg gtttagatgg taatgaaggt ttttgtgacg cctgctttac cgggaattat
     1021 cctgtagaga taacagactc aaaggtccaa ctaaaacagc tacaactgtt tgataggtaa
     1081 agagaaaaaa ctaaatcgga aataccaaag agggtttgag aggttactta aataagtagc
     1141 ggtatttgtt ggaaactgta aggcttttga caaccaattc tgcgacatat gcatgcccta
     1201 aggcattcca atgtccgtca actttatagt aaagatccgg ccggtcctca tattccggaa
     1261 ctcttaggct ctcaacatta taacgcttaa gaagattttc taatttggtc aagcggtttc
     1321 catttatata aacaattata gctaaaacag gccaattatt ttgtttacag atttcgagta
     1381 cctcctgcca tagccggaac gtcaaccttt cttcgaaacc gttgtctatc ccactagaga
     1441 cattaactat gtcctttggg gtatttcgat atgatgctaa taaataaaat atatgtctcc
     1501 ctaatcctaa tatgtatgag tttgcaatga agggtataaa ttcgataact ttctgaattc
     1561 ttctttcaaa gctaggcgga ggaacaggga gttctaccaa actatcagat ggagatagag
     1621 tgaacaattg ctctttaaca ttgtcgttga aatcattatc catgaactga agtacgacta
     1681 ggcgtgggtt aaatcttttt acttcgtttt ttagaaattt aacccagtgt gcattacccg
     1741 tgttctcaat ccccgcattc actacagaaa ttcgagtttc accgaattgt tctgcgagtt
     1801 gcttccttat gagttccggg aattcctcgc cgtcgctaac gccataccct tctgtatacg
     1861 aatcgcccaa aaaaagtatc ggtctataag gaaaggtttt cggctctgga ccccgaaaac
     1921 ctaaagaatt tgtagtatat tggataataa actctgatgt aatccattta gctgtataac
     1981 ttttcttgag cctactacca taaataggat cgtagatagt aatggtatgt ccaataatac
     2041 caacaggtac aaataaacga agaataattt cacagagaat taacgcaaat aataaaccga
     2101 caactaccag acttagtttt ttataattaa accttcttga actaatttcg agcatagcta
     2161 ttacaatctc accattaacc agtaattatt caaaaacttc tgaagttcct tgtaaaatac
     2221 agctagccat ctcagcatat aatttatacc gttgatacct aaatcgaact cgatctttcc
     2281 tctctcctta gctaatggca tgcagcataa ccttgtaagt tataattaat tttatctcaa
     2341 caaatataag tataaccaaa aggaatgcaa gcattcagga attagtttcc ttttaagttc
     2401 aacagaattc tgtattctga actcacttta actgcaactt atttaagctt tgcctttaaa
     2461 gcacaaaacg ggtacctacg gtgttgggca caaacgcctc gggtaactca cgagcaatgg
     2521 cgtgagccgc cttccagccc gtacagtgcg caggaactat tatcgcaggg ttgagcgctt
     2581 tgagcgcagc aacagtcggc ggtattatag gttcaaacaa cctaccgcta aggtgaaacc
     2641 cttctcttac cgcatacacc ttctggatac cggagagttc ctgagcatga cggatggtgt
     2701 tgatcgcccc tgcatgccca cagccagtga caaccactag ccccctgcct ttgacgttca
     2761 gcaccaccgc ttggtcatcg tgtatcaagg gatcttgctg ccacttattt ccgatgtacg
     2821 catagtggat ggaaaagcct tgctcgaatg gagttgaacg atgaatctgg ccagtcacaa
     2881 gcaccagacc acctagcaaa tacgatggac ccctctcttc gatgatctct atgccttcct
     2941 gtgttagtat accccggtct ggtggaggaa gaccgagttc atgaccgtcg ggaaatacta
     3001 ccttacactt aaggaacgca tcagggtgca acaacaatgg catcttacgc ttccccaacc
     3061 gttttatcat gcctacaagc ccctgtgtgt ggtcagtgtg cccatggctg agcacaattg
     3121 cgtggaactc ttcaggattc acatcgagca catccatatt gtgtatgagt ccgtttttac
     3181 tcaagcccgc atcaaaaagc agggattcag atgtattgcc actaatgaca gtaaccaacg
     3241 ctgcaaaacc atgctcggct acgagagtct ctctggtgag agcatccgga gttagaggca
     3301 cgcgtttaac ctggtcagtg ctcggcaata gtaaatcgag ggaattatcc aatattgtaa
     3361 ggatctcaac tttgtctgcc tctttaagtt ggatagtggt cattggtaag ccctctctat
     3421 taaaattgat ctctcttttc tattgttggc tgtattaatc gtttcagaac caatctaatc
     3481 cacgacagat tctcgatttg cctcagtgct tatgtagtta attcagcata actattataa
     3541 gaaaaatatt tcgcctaatc atcaggtaaa gtaaattatc caaaatatga gttatttatc
     3601 agcaacccta cccaataagt gacattacgc catttttaaa ctaagcagga caaacttagg
     3661 atatctacta cccatcagtt ttctaagctc attaccatta aaaaagaggc gtgcctcatt
     3721 tatctctccg ttctcaacct tgaaaaattc agcagctctg attactccgg caggactcat
     3781 cgtgtcgcaa tcgtagagaa gcactgaccc gttctcagaa aagaagtttc ttggcggtgt
     3841 aactcctttt aacatcctct gaatctgagt gcgcgtttta acgaaatcgt ccgccctacg
     3901 aaatttgtca atgctaccct gaaaatgtag atcgttatca aggtaacttc tagcctctgc
     3961 aggtttacct ttagtccatg aattatggta tgcctcaact acatgtcttg cacccattta
     4021 cctctttctt agtcttttct tctttcttac tttaccttaa aatttaccca cttatggaaa
     4081 tttatatgtt tgactgtagc taaccctcaa gctttaatca aaaaacgtaa ttattaaacc
     4141 atgacattaa aaaattttta tttgtactca tacattgtac tcatacatct ttttgacatt
     4201 attttcagca tatgttagga aaattatgat actataaagt aagtaaacat ttataatttc
     4261 agataacaaa aatttgattc aggagagagg aacgttacat gtcgattcta aacttcataa
     4321 taaaggactt taattatacg atcttgagtg caatttcatt attatcaact ttgataatct
     4381 caatcagttg tgcaaaggtt gaggtttatc ctgtgcaaaa tcaatacggt agcaaaggta
     4441 aaatcctaac agcctctggt cccggagttc gtttctacag gccagcgtta tacgtttgga
     4501 ttacaa
//
LOCUS       DATDRL010000233         7215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1346298, whole genome shotgun sequence.
ACCESSION   DATDRL010000233 DATDRL010000000
VERSION     DATDRL010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346298"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VI935_07905"
     CDS             <1..844
                     /locus_tag="VI935_07905"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLE25559.1"
                     /translation="TKQLYYSILLAKKDVEITKQNLETSKTLLSIIERRVELGESREL
                     DFIKSRVETLNFERELIRAETNLGAVKAILNRFLGDSLEKDFNLEDELTSPSTIYALD
                     NLAQRVADFNPQIKAQETEVKRAGFSLLEQIHLRVPDIFLQGFYEREIDRISTGGGVG
                     INIPLWYQNQGGIQSARAEERRASFDLKLLEIEIGNRLEDEYKNYKITFEQSKIFSAE
                     LLDRAKRSLEIAQFSYEQGEIGLLDVLDALRTYRDMLREYYQVLYEFYISQITLEKIA
                     GGKI"
     gene            844..2043
                     /locus_tag="VI935_07910"
     CDS             844..2043
                     /locus_tag="VI935_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLE25560.1"
                     /translation="MVTQSFLKPKTKKLLLSLLVSVFLISFTSCDREGEAGNEKEANE
                     DKPSNLIQLDSKSIEAANLKIEEVSLRSLKSVLEVPGKFQFNENKLAHVGSRVPGRIV
                     EVRANLGDNVKEGDSLAIIDSTELGTAQSEYLKAKANLLAQEKSYQRAKKLLEGKAIS
                     LGEFQKREAEYLTVRAEFNAAEDRLHLLGLSEAELKRIGSEHIINSKVAVRAPFSGTV
                     VERHATLGEVIEPAANLFTVADLSKLWVIADVPEKDIPLVMTGLPVEIKVSPYPNEVF
                     KGTVTYVGDQIDPSTRTVKVRTEIDNPSGKLKPEMFATIFITTQVMSDVLAIPGEAVQ
                     TDGNKKIVFVNKGNGGFERREVSLGRQLDSFYQVVNGVKAGEKIVTKGSFLLKSEAQK
                     GELGEEH"
     gene            2045..5167
                     /locus_tag="VI935_07915"
     CDS             2045..5167
                     /locus_tag="VI935_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLE25561.1"
                     /translation="MIDKIITFALQQRFLILVFTALFIGIGVYSAKKLPIDAFPDVTN
                     IQVQIITQAEGMAPTEVERLVSFPIEVTMTGLPKVTEVRSLSKIGLSVVTIVFEDDVD
                     IYFARQLVFERLQQAKENLPPGIDAEMGPISTGLGEIYQYEVDGDGYNPMELRTIQDW
                     IIRPILRTVPGVTDVNSFGGLVKQYQVLINPQKLISHNLTLREVFEAVEKNNANAGGN
                     FIEHNSEQYIVRGLGLVKTTEDLKNIIITSRDGTPIFVKDVANVMLGPEVRQGAVTKD
                     GKGEVVTGIVLMLKGASGREVVTGVKEKVKEIQKALPPGVTIKSFYDRTDLVKRAIGT
                     VTKALEEGAIFIFLVLLLFLGNIRSALIVGAVLPLSALFTFIMMERFNLSANLMSLGG
                     LAIGIGMLVDGAVVMVENVYRHLEENPEGRGNITHTVLEAAKEVGRPIVFGISIIIAV
                     FLPLFTLQGIEGKMFSPMAFTVSFALLGSLIFSLTVIPILCTFLLGEKRSRFVNIKVF
                     DRLGGLFESLSNKIITSLKNLYIPILRKALSRRKLVVGGAALALIISLSLFPFIGTEF
                     LPQLNEGSIALQAFRLPSISLTESLEVSGQIERALMKFPEVETVVSKTGRAEIASDPM
                     GVEISDIIVNLMPQSDWKIANSNEELVEKMREDLSLIPGVAYSFSQPIALRVDELISG
                     VKAQIAIKLFGDDMDVLKNKAEQIREIMSKIKGVEDLNVERVSGLQYLQIDIDRSQIA
                     RYGINVSDIQEIVETAIGGKVATEVFEGQKRFGVLVRFPEDVRKDVNTIKNILVSTPN
                     GARIPLEQLARVYLEEGPAQISRENGQRRIVIECNVQGRDIGGFVAEAQKKIASKVQL
                     PPGYFIDWGGQFENQQRAMARLYIVVPLVILLIFLLLFSTFNSLKNALLIIMNIPFAV
                     IGGVLALFISGLYLSVSASVGFIALFGVAVLNGVVMVSYFNQLRREGVPLEEAIIRGA
                     ELRLRPVLMTALVASLGLVPMLIATGPGSEIQRPLGTVVIGGLISSTILTLVVLPTLY
                     GWFEKKEVEF"
     gene            5190..5552
                     /locus_tag="VI935_07920"
     CDS             5190..5552
                     /locus_tag="VI935_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HLE25562.1"
                     /translation="MKEIKAFIQPFMLSKVVRTLQQIPEFPGMSISEVKGFGRGKAKG
                     EPRRIIEDLVEYTPKVRIEIIAKDRMVDEIVALIQKNAHTGNKGDGKIFVSNVERAVR
                     IRTGEKDEEAIWSSLKDE"
     gene            5600..6631
                     /locus_tag="VI935_07925"
     CDS             5600..6631
                     /locus_tag="VI935_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HLE25563.1"
                     /translation="MTLVHDDSDRSGHQYNHIHKHSSHAHTHGMIDPSIVSNKRGIWA
                     IKWSFIGLFVTALFQIVVVWFSGSVALLADTIHNFGDAATAVPLWIAFVLTRIKPSRR
                     FPYGLGRIEDLAGVVIVLIILSSAIIAGYEAIDRLIHPRKVEYLWAVITASIIGFLGN
                     EAVATFRIKVGKEIGSAALVADGYHARVDGWTSLAVLFGALGVWFGYPLSDPIVGLLI
                     TIAILWVVFKSGKSIFTRMLDGVDYDTLQDIKHVSKHVEGVVGVSEVRARWIGHRLHA
                     EVNISVVPTLSVVEGHNIAKEVRHQLLHHLPHLGNANIHVDPVDENGEIYHKIVEHAH
                     DNLTPHSHY"
     gene            6700..>7215
                     /locus_tag="VI935_07930"
     CDS             6700..>7215
                     /locus_tag="VI935_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE25564.1"
                     /translation="MGDFDRLAMKYLKFFMIFILLCLLVVGIAYVIKYGAFLPKVTQK
                     RVTEVQEGELSSAEAYLNHGAEDYQNSQYEHAILDFSKAIEINPRYAKAYLNRGTVYY
                     QKGQCDEATLDYNKAIEIDPKYDKAYFNRGMVYGKCKSLYDQAISDLSKAIEINPRYA
                     EALTNRANAYAS"
BASE COUNT         2172 a         1362 c         1726 g         1955 t
ORIGIN      
        1 taccaagcag ctttactact cgattctact tgctaaaaag gatgtagaga tcacaaagca
       61 aaatctagag acctccaaaa cccttctttc aattatagaa cgtagagttg agctaggcga
      121 atctagagag ttagatttta ttaaatctag ggttgagacc ttgaattttg agagagagct
      181 aattcgtgct gaaacgaatt taggtgcggt gaaggcaatt ttaaacaggt ttcttgggga
      241 ttccctagaa aaggacttta atttagaaga tgagcttacc tctccttcta ctatatatgc
      301 cttagacaac ttagcccaga gagttgccga ttttaaccct caaataaagg ctcaagagac
      361 tgaagtcaag cgtgcagggt tttcgttact tgagcaaata catctaaggg ttcctgatat
      421 tttccttcag ggtttctacg agagagaaat agatagaatt agcacaggag gcggcgtagg
      481 cataaacatt ccactctggt accaaaacca gggagggata cagagtgcaa gggctgagga
      541 acgtagggct tcttttgacc tgaagctcct tgagattgaa atcggaaacc gtctcgagga
      601 tgaatataaa aactataaaa tcacttttga acagtcaaag atattttcgg cagaactatt
      661 agaccgggca aagcgttcac ttgaaatagc gcagtttagc tacgagcagg gtgagatagg
      721 gcttctggat gttctcgatg cccttagaac ctatcgcgac atgctcagag agtattatca
      781 agttctctac gagttttaca tatcgcaaat tacattagaa aaaatcgcag ggggtaagat
      841 ctgatggtta cacaatcgtt cttaaaaccg aagacgaaaa aattgctttt aagcctattg
      901 gtttcagttt ttttgatttc cttcacctca tgtgacaggg aaggagaagc tggtaatgaa
      961 aaggaagcaa acgaagataa gccttcaaac ctaattcaac tcgactctaa atcaatagag
     1021 gcggcgaacc tcaagattga agaggtttct ttaaggtctc ttaagagcgt actagaggtg
     1081 ccaggaaagt ttcagtttaa tgaaaataaa cttgcccatg tcggctctcg tgtccctgga
     1141 agaatcgttg aggttagagc taacttagga gacaatgtca aagaaggcga cagccttgcc
     1201 ataatagata gtacagagtt gggaacagca caatcggaat atctcaaggc aaaggcaaat
     1261 cttcttgcac aagaaaagtc gtatcaaaga gctaagaaac tcctagaagg gaaagctata
     1321 tctctcggtg aatttcaaaa gcgtgaagcg gaatacttaa ccgtaagagc agagtttaat
     1381 gcggctgaag acaggcttca cctcttaggg ttgagcgaag cggaattaaa acgtataggg
     1441 agtgagcaca taatcaattc aaaagtagct gtcagagcgc ccttttctgg aacggtagtt
     1501 gagaggcacg caacacttgg agaggttata gagccggcag caaatctttt caccgtagca
     1561 gacctatcaa aactctgggt tattgctgac gttcctgaaa aggacattcc tctagttatg
     1621 accggactcc ctgtagagat aaaggtttca ccatatccga atgaagtatt taaaggcact
     1681 gttacctatg taggagacca aattgaccct tcgacacgca ctgtaaaggt tcgtactgag
     1741 atagacaacc cttccggaaa actcaagccc gagatgtttg ctacaatttt tataactaca
     1801 caggttatgt cggatgtctt agcaatccca ggggaagcgg tccaaacaga tggaaataaa
     1861 aagattgttt ttgttaataa gggcaacggt ggatttgaga gaagagaggt ttcactagga
     1921 aggcagcttg atagcttcta tcaggtagta aacggcgtca aagccggaga aaagatagtt
     1981 acaaagggga gttttctact taaatccgaa gcccaaaagg gcgagcttgg ggaggagcat
     2041 taagatgata gataaaatca taacatttgc tcttcagcaa agattcctaa tccttgtttt
     2101 tacagctctt tttataggca taggggttta ctcagccaag aagcttccta ttgatgcctt
     2161 ccccgatgtt acaaacatac aggtgcagat aataacccaa gccgagggaa tggcgcccac
     2221 cgaggttgaa agactcgtga gcttcccaat cgaggtaacc atgacggggc ttccaaaggt
     2281 aactgaagta aggtcgctct ctaagatcgg actttctgta gttaccatag tttttgaaga
     2341 cgacgtggac atctactttg caaggcaact tgtgtttgag aggctacagc aggcaaaaga
     2401 gaatctgcct cctgggattg atgcagagat ggggccaatc tcaacaggtc ttggcgagat
     2461 ataccagtac gaggttgatg gagatggata caaccctatg gaacttcgga ctattcagga
     2521 ctggattata agaccgatcc ttcgtacggt tcccggtgtg accgatgtaa atagctttgg
     2581 tggattagta aaacaatatc aggttctcat taatcctcag aagctcataa gccataacct
     2641 gacactgcgt gaggtttttg aagctgtaga aaagaacaac gccaacgccg gaggaaactt
     2701 tatagagcat aactcagagc agtatatagt ccgtggattg ggtttagtta agacaactga
     2761 ggatttaaaa aacataatta tcacatcacg cgacggtact cctatattcg taaaagatgt
     2821 agctaatgtc atgctcggtc cagaagtacg acagggggct gtaacaaagg atggaaaggg
     2881 agaggtagta acgggaatcg ttttgatgct taaaggggca agcggtcgag aagtggtaac
     2941 cggggtaaaa gaaaaagtaa aagagattca aaaggcgctt cctccaggcg ttaccattaa
     3001 gtccttttac gacagaactg atttggtaaa aagagccatt ggaaccgtta caaaagcgct
     3061 cgaagaggga gcaattttta tattcctggt acttcttctc tttctaggaa atattcgctc
     3121 tgctctcatt gttggagccg tcttaccact ttctgcactt tttaccttca tcatgatgga
     3181 aagatttaac ctctccgcaa acctcatgtc tctcggtggg cttgctatag gaattgggat
     3241 gcttgtggat ggcgctgtag tgatggtaga gaacgtttat cgccatttgg aagaaaaccc
     3301 agaagggaga ggaaatataa cccatacagt gcttgaggct gctaaagaag taggaagacc
     3361 tattgttttt ggaatctcga taatcatcgc tgtttttctt ccacttttta cgcttcaggg
     3421 aatcgaggga aagatgttct ctcctatggc atttacggtt agtttcgcac ttctcgggtc
     3481 cctgatcttt tctcttaccg tgataccgat actctgcacg ttcctgctcg gagaaaaacg
     3541 ctcccgtttt gtaaatatca aggtgtttga tagattgggc ggattatttg aatccttatc
     3601 aaacaagatt atcacttctc ttaaaaacct ctatattccc attttaagaa aagccctcag
     3661 tcgcagaaag ctcgtagttg gaggggcggc attagcactt atcataagcc tttccctatt
     3721 cccctttatc gggacagagt ttttaccaca actcaatgaa ggctcaatag ccctacaggc
     3781 atttcgtctt ccgagcattt cacttacaga gtcccttgag gtttcaggtc aaattgaaag
     3841 ggcgctcatg aaatttccag aggtcgaaac ggtggtttct aaaactgggc gcgctgagat
     3901 agcctcagac ccaatgggtg tggagataag cgatattatt gtaaacctaa tgcctcaaag
     3961 tgattggaaa atcgcaaata gcaatgaaga attggttgag aaaatgagag aggatctctc
     4021 cttaattccg ggtgtagctt atagcttctc tcagcctata gccctcaggg tggatgagct
     4081 tatctcaggg gtaaaagccc aaattgctat aaaactcttc ggtgatgaca tggatgtgct
     4141 gaaaaacaag gctgaacaaa ttagggagat aatgtcaaag attaaagggg ttgaggattt
     4201 aaacgttgag cgtgtttccg gtcttcaata cctacagata gatatagaca ggtctcaaat
     4261 agcaaggtac ggaatcaatg tttcggatat tcaagagata gttgaaaccg caataggcgg
     4321 caaggttgca acagaggtat tcgagggaca gaagagattc ggagtccttg tcagatttcc
     4381 tgaagacgta agaaaagatg taaacactat aaaaaatatc cttgtaagca ctccaaatgg
     4441 tgcccgtatc ccacttgagc aattagccag agtctatctg gaagagggtc ctgcccagat
     4501 aagtagagaa aacgggcaga ggcgaattgt aattgagtgt aacgttcagg gaagagacat
     4561 cggagggttt gttgccgaag cccaaaagaa gatagcttct aaggtacaac ttccgcctgg
     4621 atatttcatt gactggggag ggcaattcga aaaccaacag agggctatgg caaggctcta
     4681 cattgtggtg cctcttgtga ttttgcttat atttcttctt ctcttcagca cctttaattc
     4741 tttaaagaat gcactactaa tcataatgaa catcccattt gcagttatcg gtggagtatt
     4801 ggctctcttc atctccgggc tttatttaag cgtctctgcc tcagtgggat tcatagcgct
     4861 ttttggagtt gccgtcctaa acggtgttgt catggtttct tactttaacc agttaagacg
     4921 agaaggagtg ccactcgagg aagctattat tcgaggcgct gaactgagac tgagaccggt
     4981 tctgatgact gcgcttgttg caagcctggg acttgttcca atgcttattg ccacaggtcc
     5041 tggctcagag atacaaagac cactaggtac ggtggttata ggaggactaa tttcctctac
     5101 gattttaacc ctggttgttc ttcctacact ttatggctgg tttgagaaga aagaagtaga
     5161 gttttaagac aaaagatgga gggagttgca tgaaagagat taaagcattt attcagccat
     5221 ttatgctcag caaggttgtg agaacccttc aacagattcc agagtttcca ggcatgagca
     5281 tctcagaggt aaagggattt ggaagaggca aagctaaggg agaacctcga agaataatag
     5341 aagacctcgt tgagtataca cctaaagtaa gaatcgaaat catagccaag gatcgcatgg
     5401 tcgatgagat cgtagcatta atccaaaaga atgctcatac agggaataaa ggagacggaa
     5461 agatttttgt atcaaatgta gaaagggctg taagaatacg aacgggagag aaagatgaag
     5521 aggcgatctg gtcatcttta aaagatgaat gagaaggtca atgtgagatt taagaggtct
     5581 taatggtgag gaggtcaata tgacattagt gcatgatgat tctgatagaa gtggtcatca
     5641 atataatcat attcataagc atagtagcca tgctcatacc catggaatga ttgacccttc
     5701 tatcgtttca aacaagcgtg gaatttgggc aatcaagtgg tcctttatcg ggctgtttgt
     5761 aactgcgttg tttcaaatcg ttgttgtatg gttttcaggg agcgtggcac tcttagcaga
     5821 tacaattcac aattttggtg acgcggctac agccgttcca ctttggattg cctttgtgct
     5881 cacgaggata aaacctagta gacgattccc gtatggtttg ggaaggattg aggacctagc
     5941 gggtgtagtc atagtgctaa ttatcctgtc aagcgctatt atagctggat atgaggcgat
     6001 tgaccgtcta attcatccaa gaaaagtaga gtacctgtgg gcagtaatta cagcatctat
     6061 aattggcttt cttggcaatg aagcagttgc tacttttcgc attaaggtcg gcaaagagat
     6121 tggaagtgcc gctctagttg cagatgggta tcatgctcgg gtagatggtt ggaccagctt
     6181 agctgtattg ttcggcgcat taggcgtttg gtttggttat ccactgtcag atccgattgt
     6241 gggtcttttg attaccatcg ctattctatg ggtagttttt aaatcgggga aatcgatttt
     6301 tacccgtatg ctcgacggtg tagattacga cacactacaa gatataaagc atgtatcaaa
     6361 acatgttgag ggcgtcgttg gagttagcga agtgcgggcg cgatggattg gccaccgcct
     6421 gcatgcagaa gtaaatattt cagtggtgcc taccctttca gttgtggaag gacacaacat
     6481 agctaaagaa gtacgccacc aactcttaca tcatctgcct caccttggaa atgcaaatat
     6541 tcacgttgac ccagtagatg agaacgggga aatatatcac aaaatcgttg agcatgctca
     6601 tgataattta actcctcatt cacattattg aagtctgtat ccatgcttct taggctttca
     6661 agcatataga atgggtcaga ggagattcgc tgttaattca tgggagattt cgatagattg
     6721 gcaatgaagt atctaaaatt ctttatgatt tttattttgt tatgtcttct tgtagtaggc
     6781 atcgcttatg ttatcaagta tggagccttc ttacccaagg tgacacagaa aagagttact
     6841 gaagtccaag agggagagct ttcttctgcg gaggcttacc tcaatcatgg agctgaagat
     6901 tatcaaaaca gccaatatga acatgctata ttagatttca gcaaggctat agagattaac
     6961 ccgaggtatg ctaaggccta ccttaatcgt gggactgtct attatcaaaa ggggcagtgt
     7021 gacgaagcta ccttggatta caacaaagcc atagagatag atccaaaata tgataaagct
     7081 tacttcaatc gagggatggt ttatggaaaa tgcaaaagcc tatatgatca ggctatctct
     7141 gatctcagca aagccattga gataaatcca agatatgctg aagcgcttac caatagggca
     7201 aatgcttatg ccagc
//
LOCUS       DATDRL010000234         8459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3508187, whole genome shotgun sequence.
ACCESSION   DATDRL010000234 DATDRL010000000
VERSION     DATDRL010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8459
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3508187"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VI935_07935"
     CDS             complement(<1..434)
                     /locus_tag="VI935_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25565.1"
                     /translation="MEKKPHFRQLILSLIVWSMSFFGCDNPFDEGTSSQPDFEAGATG
                     FNDITDVFIEINASRDSGDVNGDGVVIIGQEQFTLDVIDSILSTDFGMEDPIPQTDNL
                     LVGVDFVCTPAFAELTVVLSPGDSVTTIRLNECEKNNPDEFE"
     gene            complement(474..881)
                     /locus_tag="VI935_07940"
     CDS             complement(474..881)
                     /locus_tag="VI935_07940"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HLE25566.1"
                     /translation="MGKTMFMALIPVIVFFMIFSNARATDWVHVATAEKNKVKAYVDV
                     DSITEDGNKRRFWMHHDFKRVQSLDENPIEKAFGHDVVDCKDKTIQYLELIIERADGS
                     KTKSKLKPEVGTVRPDSVDEWILEFVCNYKKKG"
     gene            complement(886..1050)
                     /locus_tag="VI935_07945"
     CDS             complement(886..1050)
                     /locus_tag="VI935_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25567.1"
                     /translation="MKIKKIILAFLLGLALLASYGCAFLVGAAAGGAGGYILRDQGYE
                     VQSPVEKEGG"
     gene            complement(1546..1836)
                     /locus_tag="VI935_07950"
     CDS             complement(1546..1836)
                     /locus_tag="VI935_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25568.1"
                     /translation="MRRLVISFFVTTSLTTIGIRAFTETEQQIDEIANMGEVTDNSNF
                     LTIKDEKGEAHLIKTIAVLRFGNDDLSLEIGKAGSSIEEKDTTIIEDKEFFE"
     gene            complement(2137..2505)
                     /locus_tag="VI935_07955"
     CDS             complement(2137..2505)
                     /locus_tag="VI935_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25569.1"
                     /translation="MRRLLLYMLIAAGLILGGLIFFVTWKQQTKQVAANAVGSAEIIV
                     GEVTLVGDALLIVKEDMSHRKYEFNASPDKLKDVKTGYRVEVVTANEGVSSLTILGMP
                     RADAELYQKWNKVINTNSTF"
     gene            complement(2641..3234)
                     /locus_tag="VI935_07960"
     CDS             complement(2641..3234)
                     /locus_tag="VI935_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HLE25570.1"
                     /translation="MRKLLLSTLIAAGLILVGVNSFAVDVVPQDVQMGQKKQDTEQGV
                     ETQSETTGTFEGAEYVVQEGDTLSEIAKEFLGSEEEWRLIAKANDIENPDKIIVGQRL
                     HMPSTQNNEMSAEMEQQTNQVAAVEITADITGQITAINGDILSVTDKVGKTHLIKITD
                     PETLERIKVGDNVNIKIEKGLALSVKKTEGKSAEFAP"
     gene            complement(3420..3839)
                     /locus_tag="VI935_07965"
     CDS             complement(3420..3839)
                     /locus_tag="VI935_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HLE25571.1"
                     /translation="MKKLAKNVSLFLALLTFPFFFLCTHIYGKNVKKNHSLKNYSTIG
                     VGSWYGANFHGKKTASGKRFNMHNYTAAHRSLPLGTQVRVVNLKNGRGVIVKINDRGP
                     YIKGRIIDLSYGAAKSIGMVRSGIARVKVEVISVPAG"
     gene            4265..5584
                     /locus_tag="VI935_07970"
     CDS             4265..5584
                     /locus_tag="VI935_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLE25572.1"
                     /translation="MRSTNYANERVSNLTLSKQITLGYAFMFFLMIFISGLSIYSIYK
                     LDKAASNIEGRHSALLNLLSSKEKEAETQKHFGFGNEMLNEAVKIIDEQIKYAYTNTF
                     TIVGIALLFGGILILMIPRVITKPVSQLVNATKLVASGTYSYRVKKVTGSNEISILNQ
                     AFNNMLEKIEYHHNELEKINEKNLKLLEETRRFNEVLEAKVEEATIEIKEKHEELIKA
                     ERLAIIGEIATGIAHEIRNPLSGIAVALELMKNEIQNPEEHKQTISDILREISRLERI
                     IKDLLKFAHLSYPQSLTLIECEPNEIVERSLGLIYIKAKEKGINVEKKLNCSERFKVD
                     PEQIQQVVINLLINGVEAMDKPGRLTIETGSSNGHVLIEISDTGCGFSEEDKEKIFRP
                     FYSNKEHGTGLGLPISRRIVEIHKGKILASSEKDKGSRFTVMIPTNL"
     gene            5647..7026
                     /locus_tag="VI935_07975"
     CDS             5647..7026
                     /locus_tag="VI935_07975"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLE25573.1"
                     /translation="MSELILIVDDEKFLCRQLEKALSQEGYSVISASTGKEGAEIAKK
                     ESPAVILLDLKLPDADGLEILQIISTLEPHPTTIIMTAHGNIEVAVSAIKIGAYDFIE
                     KPFHVDKLKVIIRNAISAIQLRRNLSTATSREQEKYGFNSLIGKSEAIKEITYLFQKL
                     ADTDPKTILITGESGTGKGLAARILHYNGVRAEKPLIELNCAAIPETLLESELFGYEA
                     GAFTDAKKMKKGIIELADGGTIFLDEIGDMGLALQAKLVKVIEERTFRKIGGTRDIRV
                     DTRVIAATNHDLKELVSKNLFREDLYHRLNVINIKMPPLRERKEDIPLLADYFVAYFN
                     SEAHKDINLVPEEVSEAFLNYHWPGNIRELRNTIERAVFLSEGKQINPRHLQLEEKSE
                     DGIKLEKSEGKMILEIPLDDASLTMIEEKIIREALDLNDWNQKKTAEMLGITREVLRY
                     RMKKMGLLD"
     gene            complement(7042..7266)
                     /locus_tag="VI935_07980"
     CDS             complement(7042..7266)
                     /locus_tag="VI935_07980"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HLE25574.1"
                     /translation="MEIQPFGKVLIFLGIFFALLGLLTVLAPKLPYIGRLPGDIYIKR
                     GGITIYFPIVTSILISIILSLILNFIFGKK"
     gene            complement(7308..>8459)
                     /locus_tag="VI935_07985"
     CDS             complement(7308..>8459)
                     /locus_tag="VI935_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE25575.1"
                     /translation="HRAADILKRSSHIDKAQKLYERILNMDSKDELALRRLTEFNVED
                     GAVDQAIDIYERMLRSNPNDFKILKKAIEVYRGLGDIKKLKIYLRRLASAYKLKSQDT
                     DIKEVYEEILSLDPFDPEAIEYMSQERITNELRIQKPLEEQMDAVIQNDFTNVEDIKE
                     FSSPDQYSSLVDRYSKGDSNFENVLDEEDNAKELEGSPTHLSNHQEIFSKLTLDDNKI
                     SEMEVVDKTQLEDIKEGEAEVFGVLNNKGDNQEVEESREVTSELNGLSSATLNKVGKS
                     EFFELGMMHKDKMMFEQAASEFRKSIMEGYKVWESYMMVGICFLKRGDGKTALQWFQK
                     GLFLDGLLVNQLIALKSGSAIALGILGNYSDAIKVLEGIVAEYEKYEMR"
BASE COUNT         2623 a         1724 c         1473 g         2639 t
ORIGIN      
        1 tcttcaaatt cgtcaggatt attcttttca cactcattaa gccgtatcgt tgttaccgag
       61 tctccagggg ataaaacaac tgttaactca gcaaatgcag gggtgcagac aaaatctacc
      121 cccaccaata gattgtctgt ctgtggtata ggatcttcca taccaaaatc tgttgagagg
      181 attgaatcaa ttacatccaa agtgaactgc tcctgtccta ttatcactac tccatcgcca
      241 ttcacatcac cagagtccct tgatgcatta atttctataa acacatccgt aatatcgtta
      301 aagcccgtag caccagcttc aaaatcaggt tgagaactag tcccctcatc aaaaggatta
      361 tcgcatccaa agaatgacat tgaccaaaca atcaaagata aaataagctg tctaaaatgt
      421 ggtttcttct ccattcagtg tcgctccagg gtgcttctta ctatattggt ttcctatcct
      481 tttttcttat aattacaaac aaattctaag atccattcat ctacactatc aggtcttact
      541 gttcctactt cgggctttaa cttactcttc gttttacttc catcagcgcg ttctataata
      601 agttccaagt actgtatagt cttatcttta cagtcaacaa catcgtgacc gaaggctttc
      661 tctatgggat tttcatctaa agattggact ctcttaaaat catgatgcat ccaaaacctt
      721 ctcttattac catcttctgt tatactatcc acatccacat aggcctttac tttattcttt
      781 tcagccgttg ctacatgtac ccagtctgtt gctctggcat ttgaaaatat cataaaaaat
      841 actatcaccg gtatcaaagc cataaacatt gttttcccca ttgcgttacc ctccttcctt
      901 ttcaactgga gactgtacct catagccctg atcccttaaa atatatccgc ctgcaccgcc
      961 tgctgctgct cctacaagaa atgcacaccc gtagcttgcg agaagcgcca agcctaaaag
     1021 aaatgccagg attatttttt taattttcat ttttccttcc ttactttcca tctttaaagt
     1081 ctgtccttta accatttctc tacccacaac tttgtctttt cagccaattc tttactcgac
     1141 acataagata cgtgcttgtc gttaagataa atcccccaat aggtaatcgc ggattgtttt
     1201 ctttaatatg ttcttcctgt tcattcccaa ctgggagcgc catgcagtcc catcttggat
     1261 aaactgtaaa aggagttatc gttattttca tcttcaaatc cctgctaatt cttatagaaa
     1321 gttatgccat taacttaaaa gcaatatcta tgccaacagg tttaatattc ttatcttcaa
     1381 tgtttatagg agattataat tagagggatc tttcagaaat gaagaatgca acccgctgtg
     1441 taataataca ctgcaaaaac tgagtaaatt tacacaaaaa tgcaggtaag atttttataa
     1501 tagacttagc tttttcattc ctgctggtga tttgcttata tgagcttatt caaagaattc
     1561 tttgtcttca ataatcgtag tgtctttttc ctcaattgaa gaacccgctt ttcctatttc
     1621 caaacttaga tcatcgttgc caaacctaag tactgcgata gtcttaatta gatgagcttc
     1681 gcccttctcg tccttgatgg ttaggaaatt actgttatca gttacctcac ccatattggc
     1741 tatctcatct atttgttgct ccgtttcggt aaacgctctt attccaatag tggtaagact
     1801 tgttgttaca aaaaaggata taactaatct tctcatttta gcttcacctc ccttcttatc
     1861 tcgatatata gaggttgtag ctttacttga agccacaacg tttgcgcaag ttagatggaa
     1921 gaaaacttat gaggagtgag aggtgattaa ataatttgtc aactcacctt tccttttact
     1981 caaaagtgct taaatcatta gaagagaggt aagttaagca aattttactt acccctcttt
     2041 ccagtttaaa ttctatttgc ctttagattg gttatctaat aaagataact caacatatgt
     2101 acttaacata caaatgacag gcttcgttat gccctattaa aaagtcgaat tggtattaat
     2161 aaccttattc catttctgat aaagctcagc atcagcccgc ggcataccca atatagttaa
     2221 tgacgaaact ccttcattgg ctgttacgac ttctactctg tatccagttt ttacgtcttt
     2281 tagcttatcg ggagaagcat tgaattcata tttcctgtgg ctcatatctt ctttcactat
     2341 gagcaaggca tcacctacta gagtaacttc acctactatg atttctgcag aacctacagc
     2401 attcgccgct acctgctttg tttgttgctt ccatgtaaca aaaaaaatta gtccgccaag
     2461 gataagccct gctgctataa gcatatataa aagtaacctt ctcattttgt cttacctcct
     2521 aatagttttt tatggaaagt taaatagcta aatataaagt tttaccgaca cggatgaagg
     2581 agaggtgagt aagtttgggg tattctactc acctctcttc tagagtttaa tttaattggc
     2641 ttaaggggcg aattcggctg atttaccttc agttttcttg actgaaagtg ctagaccttt
     2701 ttctatcttt atattaacat tatcaccaac cttaattcgt tccagtgtct ccgggtcagt
     2761 aattttgatt aaatgagttt tacctacttt gtccgtgaca cttaagatat ccccattaat
     2821 ggcagttatc tgccctgtaa tatcggctgt aatctcaact gcagctacct ggtttgtttg
     2881 ttgctccatt tcagcactca tttcgttgtt ttgtgtggaa ggcatatgca aacgttgtcc
     2941 aacaatgatt ttatctggat tctcaatatc attcgcttta gctatgagcc tccattcttc
     3001 ttcagagcca aggaactcct ttgcaatctc tgataaagtg tcaccttctt ggacaacata
     3061 ctcagcacct tcgaaagtac ctgtagtctc gctttgtgtc tcaactccct gctctgtgtc
     3121 ttgcttcttt tgtcccattt gaacgtcttg aggaaccaca tccacagcaa atgagtttac
     3181 tccgacaaga ataagccctg ctgctataag cgtggataaa agtaatttcc tcattttgtc
     3241 ttacctcctt cttattttag cgtttttcta tttttattgg caatgtctat gccaaatatt
     3301 actctattta atgtttatag gaggttgtgc atagtagggt cattaagaga aataaaaatg
     3361 tgtaataaca cactgtgcaa actgtgtgat cacccacatg atttgctgaa tttagtttct
     3421 caccccgctg gtactgaaat tacctcgacc tttaccctcg ctattccact tctaaccatt
     3481 ccgatagatt tagccgctcc ataagaaagg tctattatac gtccttttat atatggacca
     3541 cggtcattta tttttactat aacacctctt ccgtttttta agttaacaac tctaacttgt
     3601 gttcccaggg gtaagcttct gtgggcagcc gtgtagttgt gcatattgaa acgcttaccg
     3661 cttgctgttt ttttaccgtg aaagttggca ccataccatg aaccaacgcc gattgtagag
     3721 tagtttttga gtgagtgatt ttttttgaca ttcttcccat aaatatgcgt acacaaaaag
     3781 aagaatggga aagttagtaa agccagaaaa agtgatacgt tttttgctaa ttttttcata
     3841 cctctacccg ttggtttaaa ccgtagtggc ttcagattat aacagtatga acctcgatca
     3901 aggtgttaga taagaagccc ctctacttag acaatccagt cactatatgc tgcttttccc
     3961 tattcattgg tatcttttgt atatgtttag caactaccat gccagccagt ttaatctcgt
     4021 taatctctaa tgtttacaag aggtttcaaa taaaaattca tcaataaaaa taaaaatgca
     4081 ccttgctgtg taataacaca ctgtaaaagc tgtgcaaaat tacacaaaaa tggcaagagt
     4141 gcgtatcaat actcaaccct aatcttctta tgagatggca cagaacttgc aatacttatt
     4201 tttttaacca acttcacgca aggacctggc tatgcactat ttggtaaaaa caggtagaat
     4261 ttttatgaga tctaccaatt atgctaatga gagggtttct aacttgacgc tgagtaaaca
     4321 gatcactctg ggttatgctt ttatgttttt tcttatgata tttataagtg ggctttctat
     4381 ctatagcatc tataagcttg acaaagcagc atcgaatata gaaggacgac atagcgcatt
     4441 attgaacctt ctttcaagta aagaaaaaga agcagaaacc cagaaacatt tcggctttgg
     4501 aaacgagatg ctgaacgaag ctgtaaaaat catagacgaa caaataaaat atgcctacac
     4561 aaataccttt acgatagtag ggatagccct tttatttggt ggaatactaa ttttgatgat
     4621 cccaagagta atcacaaaac ctgtttccca gctagttaat gctacaaaat tggttgcttc
     4681 aggaacttac tcatatagag taaagaaagt aactggtagc aatgagattt ctatactcaa
     4741 tcaagccttc aacaacatgc ttgagaaaat tgagtatcat cataatgaac ttgagaagat
     4801 aaatgagaaa aatttgaagc ttcttgaaga aacaagaagg tttaatgagg tgctcgaagc
     4861 aaaggtagaa gaagcaacaa tagagataaa agaaaaacat gaagaactca taaaggcaga
     4921 gaggcttgct ataataggag agatcgcaac aggaatcgct catgaaatca gaaatcctct
     4981 ttcagggatt gccgtggcat tagaactcat gaaaaatgaa attcaaaacc cagaggagca
     5041 taagcagacg atttctgaca tcctaagaga gataagcaga ctcgaacgca taataaaaga
     5101 cctacttaaa tttgcgcacc tttcatatcc tcagagttta actctaatcg agtgtgaacc
     5161 gaatgaaatc gtagaaagat cccttggctt aatatatata aaagctaaag aaaaaggaat
     5221 caacgttgag aagaaactca actgcagcga gcggttcaaa gtagaccccg agcagattca
     5281 gcaggtagtt ataaaccttc taatcaacgg cgttgaggct atggataaac caggaaggct
     5341 tactatcgaa actggaagtt ctaatggtca tgtactaata gagatttcag acacaggctg
     5401 tggtttttct gaagaggata aagagaaaat ctttcgcccc ttttattcaa acaaggagca
     5461 tggcacaggg ctaggtcttc ccatctctcg gagaatcgtt gaaatacata aaggcaagat
     5521 tcttgcatcg agtgaaaaag ataaggggtc tagattcact gttatgattc ctacaaattt
     5581 gtgaataagg acttaagcct atattagagt gatgtaatta aatgaatttt gatttatagg
     5641 taagaagtga gtgaacttat cttaatagtg gatgatgaaa aattcctctg ccgacaatta
     5701 gagaaagctc tttctcaaga aggatatagc gtgatttctg catccacagg gaaagagggg
     5761 gctgaaattg ccaagaaaga aagtcccgct gtcattcttc ttgatttaaa actcccagac
     5821 gcggacggtc ttgagattct tcaaatcata agcacactcg agcctcatcc tactacaatc
     5881 ataatgaccg cccatgggaa tattgaagta gccgtatccg caatcaagat tggtgcttat
     5941 gattttatcg agaaaccctt tcatgttgat aagttaaaag tcataataag aaacgcaata
     6001 agcgccattc agcttagaag gaatctcagc actgcaacaa gtagagaaca ggagaaatac
     6061 ggatttaatt ctcttatagg aaaaagcgaa gctataaaag agattacata cctgtttcaa
     6121 aaattagcgg ataccgatcc taagacgatt cttattacag gagaaagtgg tacgggaaaa
     6181 ggtcttgccg caaggatcct tcattataat ggggtaagag cagaaaaacc ccttatagag
     6241 ttaaactgcg ctgccattcc ggaaacactt ctagaaagcg agctattcgg ctatgaggca
     6301 ggtgctttta cagatgcaaa aaagatgaaa aaagggatta ttgagctagc ggatggagga
     6361 acaatctttc ttgatgagat cggtgatatg ggtcttgctc ttcaggcaaa gctagtaaaa
     6421 gtaatcgagg aaagaacatt caggaagatt ggaggaacaa gggatataag agtggataca
     6481 agggtgattg ctgcaacaaa ccatgatctc aaggagttag taagcaaaaa tcttttccgt
     6541 gaggatttgt atcatcgctt aaatgtaata aacatcaaga tgccaccgct tagagagaga
     6601 aaagaggata ttccactcct tgctgattac tttgttgcat atttcaatag cgaagctcat
     6661 aaggacataa atcttgttcc agaagaagta agtgaagcgt ttcttaacta ccactggcct
     6721 ggaaacatcc gtgaacttag aaacacaatt gaaagggcag tgtttttaag tgaaggcaaa
     6781 caaataaatc ctagacatct tcagcttgaa gaaaagagcg aagacggaat aaaactcgag
     6841 aaatctgagg gtaagatgat tctagaaatc ccacttgatg atgcatcact cacaatgata
     6901 gaggagaaga ttatcagaga agcattagat ctaaatgatt ggaatcagaa aaagaccgct
     6961 gaaatgcttg gcattacgag agaagttcta agataccgaa tgaaaaaaat gggcttgctg
     7021 gattaaatac tcacctattt cttacttctt cccgaaaata aaattaagaa tcaaggacag
     7081 aattatgctt attagaatcg atgtgacaat gggaaagtaa atggttatac caccgcgttt
     7141 tatatagatg tccccaggca gcctcccgat gtatggtaat ttgggggcta agacagttaa
     7201 taatccgaga agagcaaaga aaatgcccag aaaaatgagc accttcccaa aaggctgaat
     7261 ttccatccct ctttaaacca tttataacgg ccttaaaaga actcttacta cctcatttca
     7321 tatttctcgt attctgccac aattccctct aaaaccttaa ttgcgtcaga gtaatttcct
     7381 aaaattccaa gcgcaattgc agaaccggat tttagagcga taagctgatt gactagaagc
     7441 ccatcaagga aaagaccttt ttgaaaccac tgaagcgctg ttttgccgtc ccctcttttg
     7501 aggaaacata tacctaccat catgtaggac tcccaaactt tataaccttc cattatagat
     7561 tttctaaact ccgaggcggc ctgctcaaac atcatcttat ctttatgcat catccctagc
     7621 tcaaagaatt cgctttttcc taccttattt aacgttgctg agcttaaacc atttagctcg
     7681 gaagtaactt ctctactttc ctcaacctcc tgattatcac ccttattatt gagtacacca
     7741 aatacttcag cctcaccttc ttttatatct tctaactgtg tcttatccac aacctccatc
     7801 tcagatattt tgttatcatc tagcgtgagt ttggaaaata tttcctggtg attactcaag
     7861 tgggttgggg aaccttctaa ttctttagca ttatcttcct catctaaaac attttcgaag
     7921 ttactatccc ctttactata cctatcaacg agagaactat attggtccgg ggatgaaaat
     7981 tcttttatgt cttctacatt ggtaaaatca ttttgaatta ccgcatccat ttgttcctca
     8041 agaggttttt gaattcgtaa ctcgtttgta attctttcct gactcatata ctcaatcgct
     8101 tcagggtcaa agggatcaag acttagaatt tcttcataga cttctttaat atcggtatct
     8161 tggcttttta acttataagc actagcaagt cttcttaaat atattttcaa cttctttata
     8221 tctccaagac cacgataaac ctcgattgcc tttttaagaa ttttaaaatc attagggtta
     8281 gatcttagca ttctttcata aatatcaatc gcctgatcca ctgccccatc ttcaacattg
     8341 aactcagtaa gtcttctgag agccaactca tcttttgaat ccatgtttaa aatacgttca
     8401 taaagcttct gagccttgtc aatatgagag gatcgtttca gtatatcggc agccctatg
//
LOCUS       DATDRL010000235        17211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_289069, whole genome shotgun sequence.
ACCESSION   DATDRL010000235 DATDRL010000000
VERSION     DATDRL010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 17211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17211
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289069"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2124)
                     /locus_tag="VI935_07990"
     CDS             complement(<1..2124)
                     /locus_tag="VI935_07990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE25576.1"
                     /translation="MPELTVSMPAYNAGKYIREAIESVLRQDGIEFELIVVDDGSQDN
                     TSEVVLSFEDPRIKLIRNKRNMGIGYCHNLVIEQSNSPLMMHVDSDDLVLPYAFKKMV
                     SKLKSDPSIGQVHCYFFEIDEDGRVTRDAFHKRRKNLLKKRGPDMDYKRELLVHGSVI
                     NHLRTYRREVFNEVGKFNEKIKYGVDYDMALRIVDKYDIKLVPEFLYCFRIHKTNTAQ
                     SLRFKNLRFLIQRLFILRKLLKSDKIHFTKEKKYNVNRFMFTGLYYALKLPNIIYFLE
                     SVRRRILSFIYWRVWSPTTNSLYNSMIDHFSWWPINLFSLNRGNKPTSEKRIAYYLWH
                     YPILSQTFIQREIAALRKSGLLVEIVADASGDLECLDENTRSLVKDTYYLYPVNKKHL
                     LRYFKYFFFKNPLSFLNLFLYAVFHKYGRYKTLKEDAIIFLKAVYLAGVLKDLHINHV
                     HSPWADVNAFIALIASKVLKVPYSVQARAHDVHRKKYSYASSEKFVNAEFIVTNTQYN
                     ESYLRSFLDKRDWGKIHTIYNGISLQQFESKLKSENTSNQTRILTVARLIEQKGLVYL
                     LKACKILEDRGYSFKCEIIGGPEEPLYINYYISLTKLYRQLGLEEWVFFLGPQPFNKV
                     LEEYRNSDIFVLPCVIAEDGSRDITPNVLIEAMAMKLPVITTNVTGIPEIVQDGVSGI
                     LVTPNDENALAEAMIKLIEDHKLREK"
     gene            complement(2194..2940)
                     /locus_tag="VI935_07995"
     CDS             complement(2194..2940)
                     /locus_tag="VI935_07995"
                     /inference="COORDINATES: protein motif:HMM:NF020604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HLE25577.1"
                     /translation="MKPHTSYTVCGTPRSGSGLLCEVLWKTGLAGRPDEYFLRKYVEK
                     YSEIWRASTSYEYLNKIIEEGTTGNGVFGIKIMWDQVGYFREKVLRIPQYMHLQTPDL
                     MGCIFPNLHYIRITRRDKVRQAVSFYKAKKTGIYNWHEGEPTQYLETLEFNFKSIDRL
                     IHNLCDWDSSWQNYFEALKISPFNVVYEDDLEHGYEGVIKRILTYLGVNIPDGLTIQA
                     KRRKQSDEHSERFVRLYLDALQDNKKIQCE"
     gene            complement(2958..4136)
                     /locus_tag="VI935_08000"
     CDS             complement(2958..4136)
                     /locus_tag="VI935_08000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE25578.1"
                     /translation="MELSILIYKDLKHGLSREKQFSLSTRNNFGILTSMSDITAVVLT
                     IGEETTQLAIDFVKKQTLPPEEIIIIRNVTPFHKALNQGASQVKTEFFMQVDSDMILD
                     ENCLEDLRKCMEKNVGIAVGHLRDPLIGRISSIRMFRKECFEKVQFKNSISPDTDFRN
                     DIWQYGWITVYVLRFFDKSNKKLWHTFGEHRPSYTPHYTFSKYLLEGRRYRYRKDLGG
                     LLWHFRELKNSGHEASLIAQIAIAHGIFLEDENDLLNPYPKNEDFDFLERFLTSSGSY
                     NVNKFKISPFDIFNPKKAFKKYYKLGIELRKANAFSAFKYCMDVLNKSRDNFIWIAKV
                     SLCHGLFSEDYSEKKFKKEYCKLNELLSEYNLLHILKKKLKRFFISIIYFFLHFLNIK
                     "
     gene            complement(4284..5048)
                     /locus_tag="VI935_08005"
     CDS             complement(4284..5048)
                     /locus_tag="VI935_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25579.1"
                     /translation="MKPIKFFGRKIRGIPPLSRKIFTSLFHTKILDGEFRWLVSLLRK
                     YGLFRAPSKLTIPQFDIDQFQIIRKVGSGGVNDIYLAKRDEKEYAIRIRKNKSKIYLK
                     RALENYYFLSRNDPRFVQLHGVDSNHAAFLCEYVPEHKKADLIRKECLKARLDLIAEL
                     VKIDISLRNSGIVIQDLCLDNLVIHNELVILVDFDSVVFLYEIEDFYKRKVYCLNYGL
                     SILSLFTSISFNKIVKQIYGESYKEIKHKDIKLEKK"
     gene            complement(5167..6282)
                     /locus_tag="VI935_08010"
     CDS             complement(5167..6282)
                     /locus_tag="VI935_08010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE25580.1"
                     /translation="MAKKILLGCYEVPGYGGASTSSYNLFEMMQNDGLDVCYLNLIDE
                     QDVDYYRYVFGENFGNPKSMTNVYNCVLKGSWHHPHPELNNLINYLSPDILVGKGYIA
                     AFLMKRAAPKKRNIFITSGCQQVRDYIAKKKAKDVISLGEIIKHAKHLPVLLPGYEID
                     AIRISDLIVTHSDMTMFLFEYFFPPSQIAKIYTDVIWSAEWIYKDALDYLRLKKPFFE
                     RNIDILFIASAWSRPEKNYKLVKKIVLQCKGLNIHIVGEVEEKLVGVKHYGLITKREE
                     LFILLGNAKTIVCTSLFDPAPGILFEASAMGCNIIASKNCGNWQICNEELLVDPFNLN
                     NFLEKIFLSLTKKFHDNIDYFLKTKSYQNLIDTILVF"
     gene            complement(6297..7034)
                     /locus_tag="VI935_08015"
     CDS             complement(6297..7034)
                     /locus_tag="VI935_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLE25581.1"
                     /translation="MDYAVRTTNLCKTFSVKSNHTTMFLLLKRKLLGGASNSRTIYAL
                     NDINIEIMKGNKIGIIGNNGSGKTTLLKVIAGLEEPNKGQVHVNGYITLLAGLGTGMV
                     DELSVEENVFLYGAIYGMERKKIKGKLHDIIEWAELQDFVGAELRTLSSGMRARLAFS
                     TMRHVETDIFLLDEALTAGDKNFKKKCEEYFENSKNNGKTFLISTHDLNFVKIFCTKT
                     LWLHKGQQKAFGETEEVLQLYIDSKSG"
     gene            complement(7036..7803)
                     /locus_tag="VI935_08020"
     CDS             complement(7036..7803)
                     /locus_tag="VI935_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLE25582.1"
                     /translation="MIHKKKYLNLLRELAFTQYKLKDQSTFFGFMWSFLNPLIMLTVL
                     FIFFRIRIGEGIEHYGIYLLIGIIQYTHFANSTSASMRVLRSMKNLTCNAIFPKEILV
                     IGSIISNTIEFIFSMIICVIIAFFLGIKVSSSLIMLPLVILLQLMMVLWVSLLLSYLY
                     VFVRDTDHLYQVFLRLLFFVTPTFYGLSFLGEGFAKNIVLLNPLTHLINFSRTIIIEG
                     RFFSIELMLLFVLINSALIYFAFKIFKKFEPKFAEHL"
     gene            complement(7885..8760)
                     /locus_tag="VI935_08025"
     CDS             complement(7885..8760)
                     /locus_tag="VI935_08025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE25583.1"
                     /translation="MAHAPKVSVIVPVYNSEVTIEECLQSLLKLNYPREDVEFIIVNN
                     ASTDRTADIIDSYREKISMLNEEKKGPSAARNKGIMSASGKFVAFTDSDCVVDKDWLK
                     YIIPPLQDDSIGIVGGKILAKHPCNNIEEFGEKIHDHNKAINEFKPPYVITMNWASKL
                     SVLKEVGLFDESFLRVQDGDLSYRILQAGYKIIYRSEAIVYHSNEKTLSGLFHKGYLH
                     GFHSVRVVKKHKIFLRQFGHGRFYLNSYIEILSSLINYMLGKNRSYSICYFTFNIGKK
                     VGKIFGSIRFSYFEV"
     gene            complement(8802..10655)
                     /gene="asnB"
                     /locus_tag="VI935_08030"
     CDS             complement(8802..10655)
                     /gene="asnB"
                     /locus_tag="VI935_08030"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HLE25584.1"
                     /translation="MCGIVGMLSFQANVTEWHTEIANSLDLIARRGPDDQGIWSDWHS
                     CILGFRRLSILDLSPAGHQPMLSQDGRYVIVFNGEVYNFRELRRKLELRGVKFRSTGD
                     TEVVLYALAHWGVKALNHLNGMFALGFYDTVENRLLLARDHAGIKPLYYMMTPKGLVF
                     ASQYDQILAHSWSRGLEVCREALAMYLDLSYIPAPYALLQNTHMLEPGSWLEVNTDGN
                     TSRGRFFEFPVYREPDLRGEEAYEAVDAAVTKAVRRHMVSDVPLGTFLSGGIDSPLVA
                     AKMRSVSNGNIPAFTLGTNGDRFDESPDAIVYARELGLDHVVWHVTPDDALGMLDDVI
                     DACGEPFADYSIFPTMLISRLARQKVKVMLSGDGGDEIFWGYVDRIASVLYKAHDFRH
                     PYWWRIALWGAKKIFRLGKGYPGPRWRSIGDWYLYRHTYNGHRLRHIFPDLPQWPAGF
                     KLFDYTGWELEQTAQWLRWNEFTGLLTRILLKVDRASMYHSLEVRVPLLDRDVLEVSS
                     RINWSSCLDMERKIGKLPLRHALAHHVHFQTQKKRGFMVPMGAWLRGVLRPVFEEAVL
                     RRSEIMGLPVRRDFLQKMFLQHLSEKVDFSWGLWRLLSLSLWEERHYRYHT"
     gene            complement(10720..11583)
                     /locus_tag="VI935_08035"
     CDS             complement(10720..11583)
                     /locus_tag="VI935_08035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE25585.1"
                     /translation="MNETAITVLMPLKNYYLNYLRKAIESVINQSCPYWYLLIIVEED
                     DFNKFREILEKELNDSRIEIIVNQGRKLAGAINTGMKYAKTDFVAILLADDMWSNNAV
                     EVLNRYIAQYAGIDFFHSSRVVIDENDNPISSVYYSKEKFSLDDFKVGSPVKHLLCWR
                     RDKALSIGGLDESLNSVGPDDYDFPWTMAERSATFKAVKKCLYYYRDHLDCFRLTTHL
                     PLSVHTSEIRKILKKHGVGFWDRIRFISNAKRSYLKQCLYKSSIDKWIKEKLGYDARR
                     GWIHIYRKDIL"
     gene            complement(11751..13331)
                     /locus_tag="VI935_08040"
     CDS             complement(11751..13331)
                     /locus_tag="VI935_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015130372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLE25586.1"
                     /translation="MLIDARTLLENKIIKTDVCIIGAGPAGITLAHEFIGQPFRVCLL
                     ESGGLELDEKTQSLSEGENVGLSYFPLETTRLRYFGGTLGKEAWCWQLDEIDFEKRDW
                     IPYSGWPFDKSHLHPFYERAQSILRLRPFAYDVEAWETKNSPSLPFIGDRVINKIIQI
                     SPSIRFGEIYRDEVERADNIHTYLYANVLDIETTYTARTVTHLQVACLQGNKFWVEAK
                     LFILATGGIENARLLLLSNKVQSAGLGNQNDLVGRFFMEHPHLESGILIPSDPCISAA
                     LYNLYNYECKVNNVPVIRALALSAQTLRHEKLVNFSATLEPSCYQVSNGVTSLELLLK
                     ALRQRNMPDGLIKHLSNVIVDINDVATAAYRKLFKGNSYPVKLFRLYNRTEQAPNPNS
                     RVTLTAERDCLGKNRVQLDWRLSPIDKSSLRRAHEIIRLELDRAGLGRLMITLDDNDT
                     TWPLSLTGGHHHMGTTRMHVDPKRGVVNENCQVHGISNLFIAGSSLFPTPGCVNPTLT
                     IVALALRLADHMKRLMIC"
     gene            complement(13810..14418)
                     /locus_tag="VI935_08045"
     CDS             complement(13810..14418)
                     /locus_tag="VI935_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25587.1"
                     /translation="MKDLNALEVFGGSGNVHTKNYVPYISSLEVWECNPKLEFRLKRN
                     LPNANIKITDSYKEIKVTSQKYYFIVVDNPIVFGEHIEHFDLFPDIFRVAADSTILIL
                     TIIPEVKEVYKKRWPTLFNEIQLARRKAFYKTNHPEKLSFDEMIDLYKNLIDENRFNL
                     EWCFTQERCFFCYRDKNYFPGYAYYLVLKINKILSQEEHNAT"
     gene            complement(14995..15909)
                     /locus_tag="VI935_08050"
     CDS             complement(14995..15909)
                     /locus_tag="VI935_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE25588.1"
                     /translation="MEIAVIIEGISEKRIARQSWIYFRDSLAEKDIHILVYHGNDEAF
                     ERPFDAMLLHVWQHWANKERFDPSRIFPIIEKYAIYRAEFPETVQIVFNHTDMSRRPY
                     ATPYWRLGDPILYRTPAYNRKELYPFPEEHIWAYEHIWGEPCFVSNTPPKYKAGFIGR
                     PSGPPGYRQSVARETAKVGIGICKESHAYSKKEYDEIMANCKIIVCPRGWGEQSQRHW
                     DAWLSGKPVLTDRECNSVEMIPGLRLQEEMHYLVFDDPKDIPDIVSDWTRPSRLDDLA
                     QIAESGRRAALSYDACGRIIEFFERAVK"
     gene            complement(15947..>17211)
                     /locus_tag="VI935_08055"
     CDS             complement(15947..>17211)
                     /locus_tag="VI935_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25589.1"
                     /translation="NKQWEVVLYARCPSIDEFPEELFFDDDIIWHQQQFGKVGHVAFA
                     YLVVSRGNLYGLNYVSDLVQRIGRRRGYKTRIEKIFKGWPHMLLNSIMTFAIENNIKK
                     IYSPTADLAIEHTDSSRNVQRELFERVYDGVVNQHFQTKKEGKWWVIDVGINRDNVII
                     SEKKQEIAEDRKTICITHDIERGLGHMDVDLTFAEFANKIWSKNLEEMLIIEKEMGVK
                     ATYNVLGCFFDEVRGRIEKDGHCIGFHSYDHKIDNYRPYLKKYHKIFRLIFGIITDKT
                     HNRYERQLDKCRQVDYRIKGYRTPRSKITPDLNGENLCYYNFEWLASSSYSLKTEMPI
                     MQNRIVKIPILFDDFDMYKSKMKYEEWEQKAIDRIKQNDFIAFGLHDCYAHYWLPHYR
                     EFLKKICELGKLKTLNEVANEVILCNGK"
BASE COUNT         5096 a         3627 c         2934 g         5554 t
ORIGIN      
        1 tttttctctc aatttatgat cttcaataag ctttatcatt gcttccgcta aagcattctc
       61 gtcatttgga gtgactaata ttccgctgac tccatcttgt acaatctcag gtatacccgt
      121 tacattcgtc gtgatgactg gcaatttcat agccatagcc tcgatcaaaa cattcggtgt
      181 aatatctctg ctgccatctt ctgcgatcac gcaaggcaaa acaaaaatat cagaatttct
      241 atattcttct aatactttat taaagggttg aggtcctaaa aagaagaccc attcttccaa
      301 tcctaactgc ctatatagtt tcgtcagcga tatgtagtag tttatatata aaggttcttc
      361 tgggccgccg ataatttcac atttgaatga gtatcctcta tcctctagaa tcttacaggc
      421 cttaagtaag tatacaagcc ctttctgttc gatcagtcta gctacagtga gtatcctggt
      481 ctggtttgaa gtattctcac ttttgagttt ggactcaaat tgctgcaaac taatgccatt
      541 gtaaatagta tgaattttcc cccaatctcg tttatctaaa aacgacctta gatatgattc
      601 attgtattgg gtgtttgtta caatgaattc cgcattcaca aacttctcgg atgatgcgta
      661 cgagtatttt tttctgtgaa catcgtgcgc acgtgcttgt acagaatatg gaacttttag
      721 aactttagat gctatcaagg caataaatgc gttaacgtct gcccaaggag aatgaacatg
      781 atttatgtgc aagtctttta atacccctgc caaatatacc gccttaagaa agattatggc
      841 atcctcttta agggttttat accttccgta cttgtgaaac accgcgtaaa gaaataggtt
      901 cagaaaagac aggggatttt tgaaaaaaaa gtatttaaaa tatcttagaa gatgcttttt
      961 attcacaggg tataagtagt aggtatcttt gacaagagac cttgtatttt cgtctaaaca
     1021 ttctagatcg cctgaggcat cagcaacaat ctcaaccaaa agtcccgatt tccttaatgc
     1081 tgctatttcg cgttgaatga atgtctggct caaaatggga tagtgccaca aataatatgc
     1141 tatacgtttc tctgaagtgg gtttatttcc cctattcaaa ctaaataaat taataggcca
     1201 ccaggaaaaa tgatcaatca ttgagttata aagactatta gtagttggag accatacacg
     1261 ccaatatatg aaactaagaa ttcttcttcg aacactttct aaaaaatata ttatattagg
     1321 aagctttaaa gcataataca atccagtaaa cataaatcta tttacattgt attttttctc
     1381 ttttgtgaag tggatcttat cactctttaa taactttcga aggataaaca acctttgaat
     1441 caagaacctt aaattcttaa atcgcaaaga ttgggcagta ttggtcttat gtattctgaa
     1501 acagtacaaa aactctggca caagttttat atcgtattta tctacgattc taagggccat
     1561 atcataatcc acgccatatt taattttttc attaaacttt cctacttcat taaaaacctc
     1621 ccttctgtaa gttcgcaaat gatttatgac tgagccatgg actaataatt cccttttgta
     1681 atccatatcc ggccctcttt ttttgagaag gtttttcctt cttttatgaa acgcatctct
     1741 tgttactctt ccatcttcat ctatctcaaa aaaatagcaa tggacttgcc caatactagg
     1801 atcgcttttt agtttactaa ccatcttttt aaaagcataa ggtaatacta aatcatctga
     1861 atccacatgc atcataagag gtgaattgct ctgttcaata accaagttat gacaatatcc
     1921 tatacccata ttccttttat ttcttatcaa ctttatcctt ggatcttcaa aagaaagtac
     1981 aacttccgaa gtgttatctt gagagccatc atcaacaacg attaactcaa attcaatacc
     2041 atcttgcctc aatacacttt caatagcttc tctaatatat ttcccggcat tataggctgg
     2101 cattgagact gttaattcag gcatatctcc ctttcgccca tcatttcttg ttccagcatt
     2161 tatgctttca gcttatacct cacataatta tcgctattcg cattgaattt ttttattatc
     2221 ctgcaaagca tccagataga gacgaacaaa cctttcggaa tgctcatcgc tttgtttcct
     2281 gcgtttagct tgaatagtta gaccatccgg tatgttcact cccagataag taagaatccg
     2341 tttaatcaca ccttcgtaac catgttccaa gtcgtcctca taaaccacat taaacggaga
     2401 gattttaaga gcttcaaagt aattttgcca acttgagtcc cagtcgcaca ggttatgaat
     2461 taaacgatca attgatttaa agttgaactc tagtgtttct aagtattgtg ttggttcgcc
     2521 ttcgtgccaa ttataaattc cagttttttt ggctttataa aatgaaacgg cttgcctcac
     2581 tttgtcacga cgggtgatcc tgatataatg aagatttgga aatatacaac ccatcaaatc
     2641 aggtgtctgt aaatgcatat attgggggat tcttaaaact ttttccctaa aataaccaac
     2701 ttgatcccac attattttta taccaaagac accgttgccg gttgttcctt cctcaataat
     2761 tttattgaga tattcataag acgttgaggc cctccaaatc tctgaatact tttcaacata
     2821 tttccgaagg aaatactcat cgggcctacc agccaatccg gttttccaca gcacttcaca
     2881 tagcaaccca ctgccgctgc ggggagttcc acagactgta tatgaagtat gaggtttcat
     2941 attcctgact tgttcgttta ttttatattc aaaaaatgaa gaaaaaaata gattattgat
     3001 ataaaaaagc gcttcagctt ctttttgaga atatgtagaa gattatattc cgaaagaagc
     3061 tcattaagct tgcaatactc ctttttaaat ttcttctcac tgtaatcttc tgaaaataat
     3121 ccatggcaca aactaacttt agctatccag ataaaattgt cgcgactttt atttagaaca
     3181 tccatacagt atttaaatgc agaaaaggca tttgcttttc ttaattcgat accaagttta
     3241 tagtactttt taaacgcctt tttcggattg aaaatatcaa aaggggaaat cttaaacttg
     3301 ttgacgttgt agctgcctga actcgtcaag aatctttcca gaaaatcaaa gtcttcattt
     3361 tttggatatg gatttaatag atcattttca tcttctagaa aaatcccgtg agcgattgct
     3421 atttgtgcaa ttaacgaagc ttcatgacca ctatttttta actctctaaa atgccagagt
     3481 aaccctccca aatcttttct atatctgtac cttctacctt ccaataaata cttcgagaat
     3541 gtatagtgtg gggtgtagct cggtctgtgt tcaccaaatg tgtgccatag ctttttatta
     3601 gatttgtcga agaatcttaa aacatatact gttatccacc catattgcca aatatcgttt
     3661 ctaaaatcgg tatctggcga tatcgaattc ttgaactgaa ccttttcaaa gcattctttt
     3721 ctgaacattc ttatgctaga tatcctaccg atgaggggat ccctgagatg gcctactgct
     3781 ataccgacat tcttctccat acattttcta agatcttcca aacaattttc atccaatatc
     3841 atatccgaat ctacctgcat aaaaaactcc gtttttactt gggaagctcc ttgattcagc
     3901 gccttatgga atggcgtgac attcctgatt atgattattt cttctggtgg gagtgtttgt
     3961 ttttttacaa aatcaatggc gagttgggta gtttcttcac cgatggtcaa tacgacagca
     4021 gttatgtcac tcattgaagt tagaatacca aaattatttc tagtggataa tgaaaactgt
     4081 ttttctctgg agagtccatg ctttaggtct ttatagatca gtattgatag ttccactcac
     4141 tgattgtacc aaagttgtga gaaagatctg caatcagcat tttactaaaa ttaggattac
     4201 aaaccttttc tgaaaaacaa aataataaaa agaggtaatt tttttcatca atatttacaa
     4261 gatcattaag ttttgttaat aattcatttt ttttctaatt taatatcctt gtgttttatt
     4321 tctttgtatg attccccata aatttgtttt acaattttat taaagctaat agacgtaaac
     4381 aagctcaata tggataggcc ataattaaga caatatactt ttcttttata aaaatcttca
     4441 atttcgtaca aaaaaacgac actgtcaaaa tccactaata ttaccaattc attatgtatt
     4501 acaagattat ccaggcataa atcttgaata actattccag aatttcgtaa gctaatatct
     4561 atttttacta actctgctat caaatctaat cttgccttta aacattcttt acggatcagg
     4621 tctgctttct tatgttcggg aacatattcg cacaagaatg cagcatgatt ggaatccact
     4681 ccatgtagtt gtacaaatct aggatcattt ctgcttaaaa aataataatt ctctaaagct
     4741 cttttcaaat aaattttact tttatttttt cttattctta tagcatattc tttttcatcc
     4801 cgtttagcta aataaatatc attcactcct ccacttccaa cttttctaat aatttggaat
     4861 tgatctatat caaactgagg gatggtcaac ttcgacggag ctctgaaaag tccgtatttg
     4921 cgcagtaagg agacaagcca tctgaactca ccgtctaata tcttggtgtg gaataaactt
     4981 gtaaagattt tacgactcag tggcggtatt cctcgaatct ttctcccaaa gaacttgatt
     5041 ggtttcataa cactcataat ccacctagat gaagaatttc cgtttttgat ttataggccc
     5101 ctaatttaaa agcattatgg aaagacgtaa tattcttgat agtgataatc tcttcgggtt
     5161 gggaagttaa aataccaaaa ttgtgtcaat tagattctgg taagatttag tcttcaaaaa
     5221 gtaatcaatg ttgtcatgga atttttttgt taaggaaaga aagatctttt ctagaaagtt
     5281 gtttaaatta aatggatcga caagaagctc ttcattgcag atctgccaat taccgcaatt
     5341 ctttgaagca ataatattgc aacccatagc agaggcttca aataaaatac ctggtgcagg
     5401 gtcaaaaaga gatgtgcaaa caattgtttt ggcatttccc agaagaataa acaattcttc
     5461 ccgtttcgtt attagtccat aatgttttac gccgacaagc ttttcttcta cttctccgac
     5521 tatatgaatg tttaaacctt tgcattgtaa aacaatcttt ttgacaagct tataattctt
     5581 ttccggtctg ctccatgcac tggcaatgaa aagtatatca atatttcttt cgaagaaagg
     5641 tttttttaac ctcaaataat ctaacgcgtc tttgtatatc cattctgcag accatataac
     5701 atcagtgtat attttcgcaa tttgcgaagg tgggaaaaag tactcgaata aaaacatagt
     5761 catatcagaa tgtgtgacga taagatccga aattctgata gcatctattt catatccagg
     5821 aagaagcacg ggtagatgtt ttgcgtgctt aataatttca cccagggaaa taacgtcttt
     5881 tgcttttttt ttggctatgt aatctctgac ctgttgacat ccagaggtaa taaatatgtt
     5941 tcgctttttg ggagctgccc gtttcatcaa aaaagccgct atgtaacctt tgcctaccaa
     6001 aatatctgga gacaggtaat ttatgagatt atttaactca gggtgtgggt gatgccagga
     6061 tccctttaaa acacagttat aaacattagt catagatttt ggatttccaa aattttcacc
     6121 aaaaacgtat ctataataat ctacatcttg ctcatcgatg agatttaaat aacagacatc
     6181 gagtccatca ttttgcatca tctcaaataa attgtaactt gatgtactag ctccaccata
     6241 gcctggaact tcgtagcatc ctaataaaat ctttttagcc ataagataaa aggaagctag
     6301 cccgattttg aatcaatata tagttgcaaa acctcttctg tttctccaaa agccttttgc
     6361 tgtcccttat gaagccagag agttttagta caaaaaattt ttacaaaatt cagatcatgt
     6421 gttgaaatca aaaaagtttt gccgttattt ttagaatttt caaaatattc ttcgcatttc
     6481 tttttaaagt tcttatcccc tgctgtgaga gcttcatcca agagaaatat atccgtttcg
     6541 acatgcctca tcgttgagaa agccaatctt gctctcatgc cagaagaaag ggttcttaac
     6601 tctgcgccaa caaaatcctg caattcagcc cactcgatta tatcgtgtaa ttttccctta
     6661 attttttttc tttccatgcc atatatcgcc ccgtataaaa aaacattttc ctctacgctc
     6721 agttcatcta ccattccagt accaagtccg gctagaaggg tgatatatcc atttacatga
     6781 acttgtccct tattaggctc ttctaatcca gctatgactt tcaataatgt cgttttacct
     6841 gatccattat ttccaataat tccaattttg tttcctttca ttatctcgat attgatatcg
     6901 ttcagggcgt aaatagtcct agaattagaa gccccgccga gcagttttct tttaagcaac
     6961 aagaacattg tcgtatggtt gcttttaact gaaaatgttt tacataagtt ggttgttctt
     7021 acagcgtaat ccaatttaca gatgctctgc gaatttaggt tcaaattttt tgaatatttt
     7081 gaacgcaaaa tatatcaaag ccgagtttat caaaacaaat agcaacatca attcaataga
     7141 aaaaaacctt ccttcaatta taatagtcct tgagaaattt attaaatggg ttaatggatt
     7201 tagtaaaaca atgtttttcg cgaatccttc tcctaaaaaa gatagaccat aaaatgtagg
     7261 tgttacaaag aaaagcaacc ttagaaacac ttgataaagg tgatctgtat ctcttacaaa
     7321 aacatacaaa taagataaga gaagagaaac ccataaaacc atcattaatt ggagtagaat
     7381 caccaacggt agcatgatta aagagctaga tactttaatc cccaaaaaaa aagctattat
     7441 tacacaaata atcattgaaa aaataaattc tatagtatta gaaataatag aacctattac
     7501 caatatttct ttgggaaata ttgcattgca cgttagattt ttcatagagc gtaaaacacg
     7561 catggaggcg gatgtgctgt ttgcaaaatg cgtatactgg attataccta taagcagata
     7621 aattccgtag tgttctatcc cctcaccaat ccttattcta aaaaagataa atagcactgt
     7681 aagcataatt aaaggattca gaaaactcca cataaaacca aaaaaagtgc tttgatcttt
     7741 gagtttatac tgagtaaaag ccagttctct taataaattt aagtattttt ttttatgaat
     7801 cataaaatta tctcatactg aaaacttaac cagtgtttta taggttagtg tttaacttat
     7861 aagctttttt cgattcctaa cttatcaaac ttcaaaataa gaaaatctta tagacccaaa
     7921 aatcttaccg acctttttac ctatgttgaa tgtaaaatag caaatagaat aactccggtt
     7981 cttacctagc atataattaa taagacttga taaaatctca atatagctat ttagataaaa
     8041 tctgccgtgt ccaaattgcc ttagaaaaat cttatgtttc ttaaccactc tcacagaatg
     8101 aaaaccatgt aaataaccct tgtggaataa gcccgaaagg gttttttcat tgctatggta
     8161 gactatagct tcagatctat aaataatctt atacccagct tgaagaattc tataagataa
     8221 gtcaccatct tggactctta gaaaactctc atcaaataaa ccaacttcct taaggacaga
     8281 aagttttgag gcccaattca ttgtgataac atacgggggc ttaaattcgt tgatggcttt
     8341 gttatggtca tgaattttct caccaaattc ttctatgttg ttacatgggt gttttgccag
     8401 aattttgcca cctacaatgc cgatactatc atcttgtaag gggggaatga tatattttag
     8461 ccagtcttta tctaccacgc aatccgaatc agtaaaagcg acgaactttc cgcttgcgct
     8521 cataatacct ttgtttctcg cggcggaggg tcctttcttt tcctcattta gcatgctaat
     8581 tttctctcgg taactatcta ttatatctgc tgtcctgtca gttgaagcgt tattcacaat
     8641 gatgaattcc acatcttctc ttggatagtt aagcttcaag agagactgga ggcattcttc
     8701 tattgtgact tcggagttat atactggaac tatcacagaa actttcggtg catgagccat
     8761 cgttgattgc ccttaatttc tggctaaatc aaaatgtgct attaagtatg ataacgataa
     8821 tgccgctctt cccatagaga caaactcaac agcctccata atccccagct aaagtcaact
     8881 ttttcagaaa ggtgttgcag aaacattttc tgcaagaaat cacggcgaac cggcagtccc
     8941 atgatttcgc ttcgccgcaa aaccgcctct tcgaagaccg gccggagcac tccccgtaac
     9001 caagcaccca tcggtaccat aaatccgcgc ttcttctggg tctgaaaatg cacatggtgt
     9061 gcgagtgcat gccgaagtgg taatttgcca atttttcgtt ccatatctag gcaggagctc
     9121 caattaatcc gtgaggagac ttccaggaca tcgcggtcta acagtggaac tcgtacttct
     9181 aacgagtgat acatgctagc tcggtctact ttaagcaaga ttctagtcaa aaggccggta
     9241 aattcattcc agcgcagcca ctgcgccgtc tgctcaagtt cccagccagt atagtcaaat
     9301 agcttgaaac ctgctggcca ctgagggaga tccgggaaaa tatgccgaag acggtggcca
     9361 ttgtaggtat gacgataaag ataccaatct ccaatacttc gccaccgcgg gcccggatat
     9421 ccttttccaa gtcggaagat ctttttggct ccccaaaggg caatacgcca ccaataagga
     9481 tgacggaagt catgtgcttt gtataacacg gaagcaatgc ggtcaacgta tccccaaaag
     9541 atctcatctc ccccatctcc ggagagcatc accttcacct tttgccgagc caagcgcgaa
     9601 attaacattg ttgggaaaat ggaataatcg gcaaatggtt caccgcaggc atcaatcaca
     9661 tcatcaagca taccaagagc atcgtctggc gttacatgcc acacgacatg atctaggcct
     9721 aactcccggg catatactat ggcatctggt gattcatcaa agcggtctcc gtttgtgcca
     9781 agggtgaaag caggtatatt cccgttgcta actgaccgca tcttggcagc cactaatggg
     9841 gagtcaatgc ctccggaaag gaaggtgccc agtggaacat cacttaccat gtgccgtctc
     9901 actgccttgg taacagccgc atccacagcc tcgtaagctt cttctcctcg taaatctggc
     9961 tcgcgataga caggaaactc gaagaaacgc ccccgactgg tgttgccatc ggtatttact
    10021 tctaaccaac tgcccggttc aagcatgtga gtattttgaa gcagggcata aggagcaggg
    10081 atataactca ggtccagata catagccaac gcttcacgac atacctctaa accacgactc
    10141 caagaatggg ctaatatctg gtcatactga gaagcgaaaa ccaggccttt gggggtcatc
    10201 atgtagtaaa gcggcttgat tcctgcgtga tctcgggcta ataggaggcg gttttctacg
    10261 gtatcgtaga atcctagggc aaacatgccg tttagatggt ttagcgcttt aactccccaa
    10321 tgagcgagag catacagaac tacctctgta tcgcccgtag atcgaaattt tacacctctc
    10381 agctcgagtt ttcgcctcaa ttcgcggaaa ttatagacct ctccattgaa gacgatgacg
    10441 tagcggccat cttgcgaaag catgggttgg tgtccagcag gcgataggtc cagaatggag
    10501 agacgacgaa atcccagtat acacgaatgc caatcactcc atatcccctg atcatcaggc
    10561 ccccggcggg ctatcaagtc caacgagttg gcgatctcag tatgccattc agtaacattt
    10621 gcctgaaacg acaacattcc tacaataccg cacacctaag ctctcctccc ttccaataat
    10681 tttatagggg atcgtgtttc aacgatctgg ctaataaacc tataatatat ccttcctgta
    10741 tatatgtatc caacctcgtc tcgcatcata acctagtttt tcttttatcc acttgtcaat
    10801 tgaagattta tataggcatt gcttcagata agaccttttc gcattcgaga taaaccttat
    10861 tctatcccaa aatcctacgc catgcttttt caggatcttt cttatctcac tcgtatgtac
    10921 actcaaagga agatgtgtag tcaatctaaa acaatcaaga tggtctctat aataataaag
    10981 acatttttta accgctttga aagtcgcact cctctctgcc atcgtccagg gaaaatcata
    11041 atcatcgggt cccactgagt tcaaagattc atctaaaccc ccaattgaca aagctttgtc
    11101 tctccgccag cagagtaaat gtttaactgg tgatcccacc ttaaaatcat cgagactaaa
    11161 tttttcctta ctatagtaaa cagagctaat agggttgtca ttttcgtcaa taacgactct
    11221 tgacgaatgg aagaaatcaa ttccggcata ttgggctata tagcgattta atacttctac
    11281 agcattattt gaccacatat catcagccaa cagtattgca acaaaatccg ttttggcata
    11341 tttcatgcca gtattaattg ccccagctag tttccgccct tgattcacaa taatttcaat
    11401 gcgtgagtca tttagctctt tttctaatat ctcgcggaac ttattaaaat cgtcctcttc
    11461 cacaataatt agtaagtacc agtatgggca actttgattg attacagatt caatagcttt
    11521 tcttagataa ttaagatagt aattttttaa cggcattagc actgttattg ccgtctcatt
    11581 cattgctaac ctctctttct caatgctcac atttgctatg actgagccca agttaagata
    11641 ctatctactg ctgcctatta tcacttcact agcgacctca ttaaagggat cgatcatatc
    11701 tataataatc caagtcttgc tgatcatcag atttaaagat gtttgcaaat ttaacatatc
    11761 atgagccttt tcatgtgatc ggccaatctc aaagcgagag cgactatggt aagcgtggga
    11821 ttcacacagc cgggggttgg aaatagtgaa ctgcctgcta tgaacaaatt ggagataccg
    11881 tggacctggc aattttcatt tacaacaccc cttttagggt ctacatgcat gcgtgtcgtt
    11941 cccatgtggt gatgaccacc cgtaagcgaa agcggccaag tagtatcgtt atcatctagg
    12001 gttatcatta agcgacccag tccagcacga tcgagttcta aacggattat ctcgtgtgcg
    12061 cgcctcaaac tacttttgtc tatagggctc aatcgccagt ccaactgtac acggttctta
    12121 ccaagacagt cgcgctcagc agtaagagtc actctactat ttggattggg tgcctgttca
    12181 gtgcgattat aaaggcggaa aagcttaact ggataggagt tccccttgaa gagcttcctg
    12241 tatgcggcag tggcaacatc attaatgtca acgattacat tgctcaggtg tttgataaga
    12301 ccatcaggca tattcctttg acggagggcc ttaagaagaa gttcaagaga ggtaacacca
    12361 tttgaaactt ggtagcatga tggttctaga gtagcactaa aattcaccaa tttctcatga
    12421 cgcagagtct gcgcggagag cgccaaagcc cttatgactg gtacattatt taccttacac
    12481 tcataattat ataaattata taaagctgcc gaaatacagg gatctgatgg aataagtatg
    12541 cccgattcga ggtgtgggtg ttccatgaaa aaccttccaa cgagatcgtt ttgattgccc
    12601 agtccagcac tctgtacctt attggacagt aacaacaaac gcgcattctc gatgccgcct
    12661 gtggccaaaa tgaatagctt cgcttcaacc caaaatttgt tcccctgtaa acaagccacc
    12721 tgcaagtggg ttacagtccg tgccgtatag gttgtctcaa tgtccaatac attcgcgtag
    12781 agataggtgt ggatattgtc tgcgcgctct acctcatctc gataaatttc cccaaagcgg
    12841 atagatgggc tgatttgtat gattttgtta atcacacggt ctcctatgaa tgggagagaa
    12901 gggctgtttt tagtttccca agcctcgacg tcataggcaa aaggtctaag ccgtaagata
    12961 gattgcgcac gttcgtagaa cggatgaagg tgggatttat caaaaggcca accgctgtat
    13021 ggaatccagt cccgtttttc aaaatcaatt tcgtcaagct gccaacacca agcctcctta
    13081 cccaaggtgc caccaaaata gcgaaggcgc gttgtctcta gtgggaaata agaaagccca
    13141 acattttcac cctcggaaag tgactgtgtc ttttcgtcaa gttctagacc accgctttcc
    13201 agtagacaga ctcgaaaggg ctgaccgatg aattcatgcg ccagggtaat tcccgccggt
    13261 ccagcaccaa taatgcaaac gtcagttttg ataattttgt tctctaggag agtgcgggcg
    13321 tcaattaaca aaggagtatt ccttactaaa gctgtgccag ttcaggagct atgagacgag
    13381 tgttacgggt agtaattacc ctgtttttgc atacttattt tgctcttatt ttgccgcctc
    13441 tactgaaatc tcttctaaat aggtagagcc ccttaattga actttgatct attcaaacta
    13501 ataaccttct tacacaccta tacctctctt tgacataact ttcaaactag aaccttttgg
    13561 aatctatttt atagtaaagt atatatagaa ggaggggaag aacttgattt tggggtatgt
    13621 gcttaagcag tatcggctaa agaaaatcat ccctcaaaat ctgatttttc ataggtgttt
    13681 cattccactg aaaagggccc ttttttatga atgaatacgg caggttagaa gcaaggttag
    13741 agtatgtctc tttgttacat tgtgtttgtg agtaatccct cttgatctag tcttttgctt
    13801 ccttatttct tacgttgcat tatgttcttc ttggcttagt atcttattaa tcttaagaac
    13861 gaggtaatag gcataaccgg gaaagtaatt tttatcccga taacagaaga agcaacgctc
    13921 ctgagtaaaa caccattcaa gattaaatct gttttcatct ataagattct tgtatagatc
    13981 aatcatttca tcaaatgata gtttctcagg gtgattggtc ttataaaatg ctttacgacg
    14041 tgcaagttgt atctcgttga agagagttgg ccatctcttt ttgtaaactt ctttaacttc
    14101 gggaatgata gttaaaatca ggatggttga atctgcagct acacgaaata tatcagggaa
    14161 taaatcaaaa tgctcaatat gctctccaaa aactattggg ttgtcaacga ctataaaata
    14221 atacttttgg gatgtcactt ttatctcttt ataggaatca gttatcttga tattggcatt
    14281 tggtaggttc cgttttaatc ggaactctaa ttttggatta cattcccata cttctaaact
    14341 tgatatataa ggtacatagt tttttgtatg aacgttgcct gagcctccaa aaacctcaag
    14401 tgcatttaaa tctttcaagt ttatacctct agcttctaac tcccttaaaa cttgatgtat
    14461 aggggctaac cgtaatatgt tacgaactct ccttagaaac gtctttataa acaggatgtt
    14521 tcgcattagc tcattgagct ttcttctttt tatcattatc ccatattatg cataagctct
    14581 ttatatcaca aaaaaatggt ctaatgctag taaaaatttc tcatgggttc catcagataa
    14641 gctcattacc tccacccctt ggactgcatc ttgtaaatag ccagtatcct ctcttttttc
    14701 ttcgtatctt tctgtgcttt atgtaggttt gctaggcatt aatttggatt tattggttta
    14761 atctctaatt ttctcataca cgcatcctaa catttagaat ctatttcttg atatggtttt
    14821 aaaaacatca aacaaaaggt atttttacaa gcctcaaaaa tatttaaacg ttctcggtat
    14881 gatgactatg agagactctt caatcagatg gctgcgtaaa gccaaatacg gtcttcaggt
    14941 atgcagtttc gactattcct acctgttcct cttctcaatc ataaggcatt tttctcattt
    15001 tacagcgcgc tcaaaaaact cgataatgcg gccacaggca tcataagata atgcggcacg
    15061 ccttccgctt tcagcgattt gagctaaatc atcgagacga gatggccttg tccaatccga
    15121 gacgatatcc ggtatatctt tcggatcatc aaataccaag taatgcatct cttcttgcag
    15181 gcgtagccct ggaatcattt ctactgagtt gcactccctg tcggtaagca ccggttttcc
    15241 agatagccag gcgtcccaat gcctttgact ttgttcgccc catccccttg gacaaacgat
    15301 tattttacaa ttggccatga tctcatcgta ctccttcttc gagtatgcat gactctcctt
    15361 gcagattccg attccgacct ttgccgtctc tctcgcaaca ctttggcgat agccgggagg
    15421 tccacttggt ctgccaataa aaccggcctt gtacttcggt ggggtattgg aaacgaaaca
    15481 cggttctccc caaatatgct catacgccca tatatgttcc tcagggaaag gatagagttc
    15541 tttccgattg tacgcaggtg ttctataaag tattggatcg cccagcctcc aatatggagt
    15601 tgcatagggg cggcggctca tatctgtatg attgaaaaca atctggacag tttcaggaaa
    15661 ttcagcccga taaatcgcat acttttctat gattgggaaa atccgagacg ggtcgaagcg
    15721 ctctttattg gcccaatgct gccacacgtg taatagcatt gcgtcaaacg gcctttcaaa
    15781 cgcttcgtcg tttccatgat agacaagaat atgtatgtct ttttcagcta gggagtctct
    15841 gaaataaatc catgattgcc ttgctattct tttttcacta atgccctcta tgatgacagc
    15901 gatctccatg tataagtaca ccctctattt atctgcccac gctaccctat ttaccattgc
    15961 acaaaatcac ttcattagct acttcattta acgtcttaag ctttcctaat tcacaaatct
    16021 ttttaaggaa ttctctatag tggggtaacc aataatgtgc ataacagtcg tgcaagccaa
    16081 acgcaatgaa gtcattttgt ttaattctat ctatagcctt ttgctcccac tcctcgtatt
    16141 tcattttact cttgtacata tcaaaatcgt caaacaggat gggtattttt acaatcctat
    16201 tttgcattat tggcatttct gttttaaggg aataagacga gctggccagc cattcaaaat
    16261 tataatagca taggttctca ccgttcaagt caggggttat ttttgatcta ggtgtcctgt
    16321 aaccttttat cctgtagtct acctgcctgc atttgtctag ctgtctctca tacctattat
    16381 gagttttatc cgttatgatt ccgaatatca gtctaaaaat tttatggtat ttttttaaat
    16441 atggccgata gttatcaatt ttatgatcat aggaatgaaa accaatgcag tgaccatcct
    16501 tctctatcct tccccttact tcatcaaaaa agcatccaag tacgttgtat gttgctttca
    16561 cacccatctc tttttcaata atcagcatct cctcaaggtt ttttgaccag atcttgttgg
    16621 caaactctgc aaaagtcaga tcaacatcca tatgccccaa tcctctctca atatcatgag
    16681 ttatacagat cgtttttcta tcttcggcga tttcctgttt tttttcggat attattacat
    16741 tgtctctgtt tatccctaca tcaattaccc accatttccc ttcttttttt gtttgaaaat
    16801 gttggttaac tactccatcg tatacccttt cgaataattc cctttgaaca tttcgagaag
    16861 agtcagtatg ttctatagct aaatccgcag tcggcgaata aatcttttta atattatttt
    16921 ctatggcgaa agtcatgatg ctatttaaca gcatatgagg ccatcctttg aaaatctttt
    16981 ctattcttgt cttatatcct cttcttctgc ctattcgctg caccaggtcg gaaacgtaat
    17041 ttaaaccata taaattgcct ctactgacaa ccagataggc aaatgctaca tgtccaacct
    17101 tgccaaattg ctgctgatgc catattatgt catcatcaaa aaataattct tcaggaaact
    17161 catctattga tggacaccta gcatataaca caacttccca ctgtttatta g
//
LOCUS       DATDRL010000236         2343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1660395, whole genome shotgun sequence.
ACCESSION   DATDRL010000236 DATDRL010000000
VERSION     DATDRL010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660395"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1967)
                     /locus_tag="VI935_08060"
     CDS             complement(123..1967)
                     /locus_tag="VI935_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLE25590.1"
                     /translation="MSGNESILWMTVIAIVAGIVAQIAAAFIGIPSIVPLLFLGIVIG
                     PEVLGFLDPKVFGNGFEAIIKLCVAIILFEAGLNLDRSEIRKHQKVIVRLISYGGLIT
                     MVLAAFFSKLIMDLSWSMAFLFGALVIVTGPTVINPLLRRVRVGARLKNILESEGVFI
                     DPIGAIIAIFVFEIILEKPPSFFHAVSLVFARLGIGIVIGVIGGFLIGKVAKRWSLLM
                     EEVGELFILASALGIYALSEAVIAESGLMAAVASGAIIGNMDIPEEDTLRKFKGKISI
                     LVISILFILLASNLKLEYITSLGWSGVFIVLALLFIVRPVEILLTTYGSGLHLKEKAF
                     LSYISPRGIVAASVSSIFAIQLDNKEFQGGDVIQGLVFLTIGISVVLQGMTANSVAKM
                     LGVLIKGRKTVIVGADSFGRLVGKLLRMKGKEASFIDTNENLVRFAYIEGFEAIEGNS
                     LDLDKLEEVGITEADTTLAVTTSNKVNLLVSRLAKEDFGIKTTLPILNQNEEGLDKET
                     VTKLGLEIAFGKPLNMYEVNPKIAHREFKLYECSVDDAIVGKKIEDLSLPTDVIAILI
                     VRSKSEIPIICASNLPIEKGDKITLVDLSDGLRGFEGVGFKDCTQINL"
BASE COUNT          757 a          514 c          364 g          708 t
ORIGIN      
        1 tctatttgaa actattaata atctctggct catgtcacac aacctttatt tcaaactcta
       61 ccaaatagta ttgcccttaa tcttttgtat tcaaccccat ggtttatctt tagttagctc
      121 tgttacaaat ttatttgagt acaatcttta aagcctacac cctcaaaacc cctcaaacca
      181 tcgcttaaat ccacaagagt tatcttatcc cctttctcaa taggtagatt acttgcgcaa
      241 ataattggaa tttcagattt gctgcgaact atgagaattg caatcacgtc agtgggaaga
      301 gacaagtctt caatcttctt tccaacaatt gcatcatcta cagagcactc atatagctta
      361 aattctctgt gtgctatctt aggattaacc tcatacatat ttaaaggttt tccaaaggcg
      421 atttctaaac cgagttttgt tacggtctct ttatccaggc cttcttcatt ctggtttaaa
      481 ataggaagtg tggtttttat tccaaagtcc tctttagcaa gtcggcttac tagcagatta
      541 accttattac ttgttgtaac tgctagagta gtatcggctt ctgtaatccc tacttcctca
      601 agtttatcca ggtcaagact gtttccctca atagcttcaa atccttctat ataggcaaat
      661 cttacgagat tttcatttgt gtctataaaa ctggcttcct ttcctttcat ccttaaaagc
      721 tttccaacta gcctgccaaa tgaatctgct cctacaatga cagtctttcg acccttgatc
      781 aataccccta acatctttgc tacactgttt gctgtcatac cttgaagaac aacagaaatg
      841 cctatagtta aaaaaacaag cccttgaatt acatcaccac cctgaaactc tttattatca
      901 agctggatag caaatataga agacacagat gctgctacaa ttcccctcgg agaaatataa
      961 gacagaaaag ccttttcttt caaatgaagt cccgatccat aagtagtaag aagaatctcg
     1021 acaggacgta cgataaaaag aagggcaaga actataaaca ctccactcca gccaagagag
     1081 gtgatatact cgagtttcag gtttgaagcc aaaaggataa acaatatcga aattacaaga
     1141 atcgaaatct ttcctttaaa ttttctgagg gtatcttctt caggaatgtc catgtttcct
     1201 ataattgccc cagatgcaac tgcagccata agaccggact cagcaatcac tgcttctgac
     1261 agagcataaa ttccaagagc agaagccaag ataaaaagct caccaacttc ttccataagc
     1321 aatgaccatc tctttgcaac cttaccaatc aaaaaaccac caataactcc aattacgatt
     1381 ccaatcccta atcttgcaaa tacgagtgat actgcgtgaa agaatgaagg aggtttttcc
     1441 aaaattattt caaatacaaa tattgcgatt atcgcaccta tcgggtctat aaagactccc
     1501 tccgactcta atatattctt tagcctagct cctactctaa ctcttcttag aagtggattt
     1561 atcacggtag gacctgttac aatcactagg gcaccaaaaa gaaaagccat agaccaagat
     1621 aaatccatta tgagtttcga gaaaaaagct gctaacacca ttgtgataag accgccgtat
     1681 gaaataagac ggacaattac cttctggtgc tttcttatct cgcttctatc aaggttaaga
     1741 cctgcttcaa agagaattat cgctacacag agttttatta ttgcttcaaa tccatttcca
     1801 aagacttttg gatcaagaaa acccaagacc tcaggtccta tgactattcc taaaaacaat
     1861 aaaggaacaa tgcttggtat tcctatgaaa gcagccgcta tttgtgccac aatacctgca
     1921 acaatagcaa tgacagtcat ccagagtatg gattcgtttc cactcaaatt agaatactcc
     1981 tctctttatt ttctcagata attcagacca aacagggatt gtcaaatatc tttatgcttt
     2041 taagccttag atgaatatta ccttaacatt attaaattag gattaaaatt tttggggatt
     2101 attagttttc tgaagagaac agaatgatac tcatttcgtt aatcctattt tgataaaaaa
     2161 aatagcagga cagccgatgg ggcgtatgct gtcctgccaa aaggaggtac tgagagacga
     2221 tggggcttta ataattctat aaagtgaaat aatagagaag ctctaaggca actgtgtcct
     2281 gagtcttctt aaaattccca tcgttatctt caaatacacc ttcgtcagac tgatcatgac
     2341 ggt
//
LOCUS       DATDRL010000237         9942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3920063, whole genome shotgun sequence.
ACCESSION   DATDRL010000237 DATDRL010000000
VERSION     DATDRL010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9942
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3920063"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VI935_08065"
     CDS             complement(<1..473)
                     /locus_tag="VI935_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HLE25591.1"
                     /translation="MKIPELSVVTGAFSYTGKYITSRLLSMGKRVRTLTGHPDRHNPF
                     GNQVSVAPFNFDNQSELIKGLQGATTLYNTYWVRFSYGEVTFDKAIENTKTLIKAAEE
                     AGVSRIIHISITNASEESSLPYFRGKGILEKAIINSKLSYAIIRPTVIFGPEDIL"
     gene            complement(854..2578)
                     /locus_tag="VI935_08070"
     CDS             complement(854..2578)
                     /locus_tag="VI935_08070"
                     /inference="COORDINATES: protein motif:HMM:NF010029.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HLE25592.1"
                     /translation="MFEEKKSKVEIAKENSNGLRGTIREELGSNSDHCSTENTHILKF
                     HGIYQQDDRDLRQELKKEGKDKKYIFMVRTKNPGGGELSPEQWEALNKITDLYGNGTL
                     RITTREDIQFHGVGKEELEGTIRLLNSELISTYGACGDGNRNTVACPVSNIRKGSLFD
                     GQKWAQLISKHLSFKSKAYYEIWLDGEPITKEEDVREPQSNDETETLYGKTYLPRKFK
                     IGIGLPDDNCVDVHTHDIGIIPLLNGGLMGFNVLVGGGMGSTHRKVRTFPRLADPLAL
                     VSPENLIDVLTRIVEIQRDHGDRSDRNHARMKYVVEELGVERFKEELERRLGYRLLPP
                     EPIELRYTESHLGWHEQDTSGLWYVGIFVENGRLKDTDQSQIKTGLREIVRRFRPVVR
                     LTPSQDIVLSNIPEEKIKDIESALRDYRIITEKEITSLRKNSIACPALPTCGLALAEA
                     ERFLPCLIDELEERGYGDEKIKIRMSGCPNACSRPPVAEVGIMGVSPKKYNLYVGGSF
                     EGTRLNRLYEELIDAGELADRIAELIDIFHLNKRPGERFGDFCNRVGVEKLKELRIKL
                     YEEHRRVV"
     gene            complement(2721..3908)
                     /locus_tag="VI935_08075"
     CDS             complement(2721..3908)
                     /locus_tag="VI935_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HLE25593.1"
                     /translation="MKYQKYEDKFEEHPIGGLFGEEEPFGSHELLEDESLQDRTEAED
                     NTQITSPRIKTQKEEGADKKLRLLQIYFKDIGNKPIFTPKEEIEVSVKIKKCELGAKE
                     IERTLKEILGKDLKESPLEVVNKSKLEAALNNRNSILRRMQRLHALRKAYLNKTRELK
                     EKFIKANLRLVIHIAKKYKGQGLPYADLIQEGNIGLMRAIETFDHTKGKFSTYATWWI
                     YQAISRALLDQTRTIRVPVYVLENVSKVHRMNSMLHKEMGRKPMPEEVAERVGIPVEA
                     VKWILEDTNSVIHLDSPIFYEEKTTLLEFVSDNGSSVPDSIVAKAAIPQRIREVLSIL
                     TPREQEIIRMRFGIGQETPHTLDEIGRKFGLTRERIRQIEHEAFRKLKNSEIRDILRS
                     FLE"
     gene            complement(3960..4577)
                     /locus_tag="VI935_08080"
     CDS             complement(3960..4577)
                     /locus_tag="VI935_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLE25594.1"
                     /translation="MSIFDSKYREKHISARIARSLFRISQAIKNMLWDECKLKNLTPA
                     QIQTLLFLRYIRPDSATVNTLSRSLSSTPATASGVLDALQKKSMILRKRKESDRRVVF
                     LSLTNKGANTIQRLEGLGYELQKIIEGLAVEEQEVLLSSLSKIERLLREKGYALITET
                     CLTCCYFRLNIHLGAGKPHYCNLFNIDMGEDEIYKECPEYSELEM"
     gene            complement(5161..5874)
                     /locus_tag="VI935_08085"
     CDS             complement(5161..5874)
                     /locus_tag="VI935_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLE25595.1"
                     /translation="MASQLPGAKIVERVDLTDDLMIIKIKPEIPLNFKPGQYCTIGLR
                     GVERPYSIVSSPHEQSLELFVELVPDGELTPKLWQVTVGEVLSIRPSAKGVFTFDERY
                     PNQFMVATVTGIAPFTSMIRNYLHLALSSHTFYILQGASYQDEFAYKDELETFSKEHE
                     NIIYIPTVSRPEEERNKGWQGETGRVNVIVERYLEKFSLDPKTTLVYACGHPGMIGDV
                     KQRLGSKGYKVKDEKFWRE"
     gene            complement(5979..6197)
                     /locus_tag="VI935_08090"
     CDS             complement(5979..6197)
                     /locus_tag="VI935_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25596.1"
                     /translation="MNCLGVDFHKNYSTVTVVDHKGQALQKAKLYNNNESFAEFLRPC
                     SEVSAVVEACRNWPVVVDLLEDLVDEVN"
     gene            complement(6769..8004)
                     /gene="fabF"
                     /locus_tag="VI935_08095"
     CDS             complement(6769..8004)
                     /gene="fabF"
                     /locus_tag="VI935_08095"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HLE25597.1"
                     /translation="MKRRVVVTGVGLITPLGTGNEKSWKGICEGYPGIRRIQKFDPSV
                     LKTQIAGEVLDLDPVDFMDPKEIRRSDPFIQYAVAATQLALNDAGLEITEDISPNVGT
                     FVSSGIGGLDSFQKNVIEMHERGPARVSPFFIPSMIANMASGYVSIFFHAKGPNSCTT
                     TACAASAHAIGDAAKIIERGDADVMIAGGTEAPILPVAIAGFNAMRALSTRNEEPHRA
                     SRPFDKERDGFVMGEGAGILILEEIEFAKRHRARVYAEVIGYGMSSDAYHITSPSLDG
                     PVRCMRRALRDAQLNPEDIDYINAHGTSTPQNDVNETRAVKEVFNSYAYRLPISSTKS
                     MTGHLLGAAGGIEAAFTVLALYNGIIPPTINLEFPDPECDLDYVPNEARELKIKTALS
                     NSFGFGGTNASLAFKRFED"
     gene            complement(8004..8255)
                     /gene="acpP"
                     /locus_tag="VI935_08100"
     CDS             complement(8004..8255)
                     /gene="acpP"
                     /locus_tag="VI935_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HLE25598.1"
                     /translation="MGIQHDQELVGKVRKMIASQLGKSEDEISLESAFIEDLGADSLD
                     LVELVMAMEDEFGVEISDEDAQRITTVQDAINYVLERRK"
     gene            complement(8283..9029)
                     /gene="fabG"
                     /locus_tag="VI935_08105"
     CDS             complement(8283..9029)
                     /gene="fabG"
                     /locus_tag="VI935_08105"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HLE25599.1"
                     /translation="MAEFKDKVALVTGASRGIGRAVAIELASRGVFVVVNYAQNEAAA
                     REVLFTIENSGGKGEIRHFDVANTTVVQDEIGKIGEQFGGLHILVNNAGITKDGLIVR
                     MKEEDWDMVLSVDLKGVFNCTRAAAKIMMKQRYGRIINISSIIGEMGNSGQVNYSAAK
                     AGIIGFTKSAAKELGSRGITVNAVSPGFIETDMTRNLPENIRNKYVEGIPIGRFGNPE
                     DVAKVVAFLSSDDASYITGEVVRVNGGLYT"
     gene            complement(9194..>9942)
                     /gene="fabD"
                     /locus_tag="VI935_08110"
     CDS             complement(9194..>9942)
                     /gene="fabD"
                     /locus_tag="VI935_08110"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HLE25600.1"
                     /translation="LTVSVAAWRVLDEEMGIKAEFLAGHSLGEYSALVASGGLDFKDA
                     VLVVRKRGEFMQSAVPDGVGAMAAILGLSKEKVGEVCIEAHKDDLIVSPANFNAPGQV
                     VISGNKEAVEEACALAKSNGAKRVLLLEVSAPFHCALMNSAALRLEEVLSQVRIHELQ
                     TAVVSNFDAKPNKDSSKVKKLLVNQVANPVRWDESVNMLYENGVREYIEIGPGNVLSG
                     LIKRTVSNASVFNFEKIEQLNSLKERWRTI"
BASE COUNT         2577 a         2387 c         1836 g         3142 t
ORIGIN      
        1 ataagaatgt cttcaggtcc aaatatcacg gttggtcgaa taatagcata tgagagctta
       61 gaattgatga tggctttttc aagaatgcct tttcccctga aataaggaag ggatgattct
      121 tcagaggcat tcgtaatgct aatgtgtata attctgctga cgccagcttc ttcggctgct
      181 ttgatgagcg ttttcgtatt ctcgatagcc ttgtcgaagg tgacctcacc atacgaaaag
      241 cgaacccagt atgtgttgta aagggttgta gctccttgca aacccttaat cagttcactc
      301 tggttatcga aattgaatgg ggcaacactc acttggttgc cgaatggatt atggcggtca
      361 ggatgccctg taagcgttct gactcttttc cccatcgaaa gaagtcgact cgtgatgtat
      421 ttgcctgtgt agctgaacgc cccagtcact acactcaatt caggaatttt cattaccata
      481 cctctggcct tgagttgcta ccttttacag taatggtata ccaagctgga ctatagactc
      541 ttttgcctta atccaaacgt atatcttgtt tcttgaatat cagtggcata atgaaagggc
      601 ctgtctttag agccaaaaaa tgctgggtga atcggatttt ctcgtgtgat aaaatctatt
      661 tttgaaaatc tttaaaacat aagaagggat ttccatgatt tgaaggggat ggcgttctcc
      721 tcgggataat ctggatgttc tttacgctgc tctttcccat tgtaactgta tcgtggcttc
      781 attctttacc tccactggat gctctaaggt ctcttatggg ccccaaattg atattcttct
      841 caatactaaa ttttcatact acccttctat gttcttcgta gagcttgatt cttagctctt
      901 ttagcttctc tacccctaca cgattgcaga agtcaccaaa gcgttcaccc ggtcttttat
      961 tcaggtgaaa tatatctatt agctctgcta tcctatcagc caactctcct gcatctataa
     1021 gctcttcata aagccgatta agccttgtgc cctcgaaact accgcctacg tataggttgt
     1081 actttttggg tgagacgccc ataataccta cctcagccac aggtggtctc gagcaggcat
     1141 tcggacagcc gctcatgcgt atcttaattt tctcatcccc gtatcctctc tcctccagtt
     1201 catctatgag acaaggaaga aacctctcgg cctcggcaag tgcaagccca catgtaggaa
     1261 gtgcggggca ggctatggag ttttttctca agcttgtaat ttccttttcg gttattattc
     1321 tgtagtcccg taatgcggat tctatatcct ttattttttc ctcagggata ttggaaagaa
     1381 caatatcttg agacggggta agtcttacca cgggtctaaa cctccttaca atctcacgaa
     1441 gacccgtctt aatctggctc tggtcagtat ctttcagacg tccattttct acaaaaatac
     1501 ccacatacca gagtcccgaa gtgtcctgtt catgccagcc cagatgactt tccgtatagc
     1561 ggagttctat gggttcagga ggaagaagcc tgtagcctag acgtctttct agctcctctt
     1621 taaatctttc aactcccaat tcttctacaa cgtacttcat tctggcgtgg tttctatccg
     1681 acctatctcc atggtctctt tgtatttcaa ctatacgtgt taacacatca attaaattct
     1741 ccggcgaaac cagcgcaaga gggtcggcaa gacgaggaaa agttcttacc tttctatggg
     1801 tacttcccat accgcctccc acaagcacat taaagcccat aagcccgccg tttagaagtg
     1861 gaattattcc tatatcgtgg gtgtgcacat caacgcagtt atcgtcgggg agaccaatcc
     1921 cgatcttgaa tttcctcggc agatacgtct tcccgtagag ggtttccgtt tcatcgttcg
     1981 actgaggctc acgaacgtct tcttctttgg ttataggctc tccgtctagc cagatttcgt
     2041 agtatgcttt ggatttaaaa ctaaggtgct tagatataag ctgagcccac ttttgtccat
     2101 cgaaaagtga tcccttccta atgttcgata cggggcaggc tacggtattg cggtttccgt
     2161 caccgcaggc tccgtaggtt gatatgagct ctgaatttag aaggcggatt gttccttcaa
     2221 gctcttcttt tcctaccccg tgaaactgaa tatcctctct tgtggtaatt ctaagcgtcc
     2281 cgtttccgta gaggtctgtt attttgttaa gagcctccca ctgctccggg gaaagctccc
     2341 ctccacctgg gttcttggtt cttaccataa agatatattt cttgtctttc ccctcttttt
     2401 ttagctcctg cctaaggtct ctatcatcct gctggtaaat accgtggaat tttagaatat
     2461 gggtgttctc cgttgaacaa tggtcggagt tgcttccaag ctcctcacgg attgttcctc
     2521 ttagaccatt actattttcc ttagcaatct caaccttcga tttcttttct tcaaacatca
     2581 ctaataccca cttactttaa gtgatttata gtcttccgcg taacagccga taaatctttg
     2641 caataaaatg tcctgtattt attaaataaa tccaatttat ttttatggaa tggcactctc
     2701 tatcattatc attggtctgt ttattctaaa aaacttctta atatatctct tatttctgaa
     2761 ttcttgagtt tcctaaatgc ctcatgttct atctgcctta tacgttcacg cgtgagaccg
     2821 aactttcttc caatctcatc gagtgtatgc ggggtttctt gacctatccc gaatctcatc
     2881 ctaattattt cttgttctct aggggtaaga attgaaagaa cctctcttat cctctgtgga
     2941 attgctgcct ttgccacaat agagtctggc acagatgacc cattgtcaga gacaaactca
     3001 agaagagtcg ttttctcctc ataaaatata ggtgagtcga gatggataac actgtttgtg
     3061 tcttctaaaa tccactttac tgcctcaaca ggaattccaa ctctctcagc aacttcctct
     3121 ggcatgggtt ttctccccat ttccttatga agcattgagt tcattctgtg taccttgctt
     3181 acattctcaa gcacatacac aggaaccctg atggttcttg tctgatccag tagtgctctc
     3241 gatatagctt gatatatcca ccaggtagca taagtggaaa acttaccctt tgtgtggtcg
     3301 aacgtctcta tagctctcat taggccgata ttaccctctt gaattagatc tgcgtaagga
     3361 agtccctgcc ctttatactt ctttgcaata tgtataacta accttaagtt tgccttgata
     3421 aacttctctt taagctctct tgtcttgttc aaatacgctt ttctaagagc gtgaagtctc
     3481 tgcatacgcc ttagaatgga attcctattg tttaatgctg cctcaagttt acttttattt
     3541 actacctcta gagggctttc ttttaagtct ttgcccaaaa tctccttaag agttctctct
     3601 atctccttcg ctcctaactc acattttttt atctttactg aaacctcaat ttcctcctta
     3661 ggagtgaaaa ttggcttatt ccctatatct ttaaagtaga tttggaggag cctgagtttc
     3721 ttatctgcac cctcctcttt ttgtgtctta atcctcgggg atgtaatctg agtattatct
     3781 tctgcctcag ttctatcttg tagggattca tcctcaagaa gttcatggga tccaaagggt
     3841 tcctcttcac cgaagagtcc accgattgga tgctcttcaa atttatcttc gtatttttgg
     3901 tatttcattc tatttttatt taggattcct aaatattaaa ttaaaaaaat ttttttattc
     3961 tacatctcta gctcactata ctctggacat tccttgtata tctcatcctc acccatatca
     4021 atattgaaaa gattacaata atgtggtttt cccgctccta gatgtatgtt cagtctgaaa
     4081 taacagcagg tcagacacgt ttccgttatc aaggcatatc ccttttctct taataacctt
     4141 tcaattttag ataaagaact taataatact tcttgttcct caacagccag cccttcgata
     4201 attttttgga gctcatagcc aaggccttcc aacctttgga tggtattcgc acctttgttg
     4261 gtcaagctta aaaaaacaac tctcctatca ctttcctttc tttttcttaa tatcatgctc
     4321 tttttttgca aagcatccag aacaccgcta gctgtagcag gagtggatga aagagaccta
     4381 gaaagtgtgt taacggtagc tgaatcaggc cttatatagc ggagaaatag aagagtctga
     4441 atctgtgcag gtgttagatt ttttaacttg cattcgtccc acaacatgtt cttaatagcc
     4501 tgacttatgc gaaaaagtga tctggctatc ctcgcgctta tgtgcttttc tctatacttt
     4561 gagtcgaaaa ttgacatata agacctaatt atttagtatt cctaaatatg taacattata
     4621 agcctagttt tgtcaagacg ggtattatcc ctttaaagaa agcgtgaaag gagaacaaga
     4681 tgagtagatt tattgaaatt gctaaaatca atgaaatagc tccaggtaag gcaaagatgt
     4741 tagaagggaa aaagatagcc atttttaacc tagaatgaga ctattatgct attgatgaca
     4801 cctgtccaca tgcaagggga cctctttctg aaggggaagt tgagggagaa gtggtcacta
     4861 gcccatggcc tgggttagaa ttcaatataa aaacaggtga attactaaga cctccagcca
     4921 gaagagggta aaaagctacg tggttcgggt agaaggttct atcataaaga tagaggctta
     4981 aagaaaacct tgcatgcctt tctttttacg acctgagtgt gcaagagctt ttattttagc
     5041 gacctttgag catccgacaa tttggaagtc tgtttccctc ttgcgaaaca atttatggtt
     5101 attttgaaat cgttatcatt ttctcaaagg aaggtcttgt gaattcatct cttattccct
     5161 tcactctctc cagaatttct catcctttac cttataccct ttactcccca acctctgctt
     5221 cacgtccccg atcattccag gatgaccaca ggcatatacc aaggtggtct taggatcaag
     5281 ggaaaatttt tccagatacc tctcaacaat tacattcacc cttcccgtct ctccttgcca
     5341 gcctttgttt ctctcctctt caggcctgct cacggtaggg atgtaaatga tattctcatg
     5401 ctctttcgag aacgtctcca gttcgtcttt atatgcgaat tcgtcctggt agcttgcgcc
     5461 ctggaggata taaaaggtgt gtgaactcag tgcgaggtgt aggtaattcc tgatcatgct
     5521 cgtaaaaggc gcgattcccg tcaccgttgc taccataaac tgatttggat acctctcatc
     5581 aaaggtgaag actccttttg cgctcggtcg aatgctgagc acctctccaa ctgttacctg
     5641 ccacaatttg ggtgtgagtt ctccgtcagg aaccaactcc acgaaaagtt ccagtgattg
     5701 ctcatgcggg gatgaaacga tggaataagg tctttcaaca ccccgtagac caatcgtaca
     5761 gtattggcct ggtttaaagt tcaaggggat ctcaggtttg atttttatga tcatgagatc
     5821 atcggtcaaa tcgacccgct ctactatttt tgcccctggc agttgagacg ccatatcaca
     5881 atcccttcta aattagagag aaataatcat tacactcatt gtatgcataa aattgtacct
     5941 gaagtctcag taggtcataa gtaaatcttg ttacacagct aatttacctc atccacaaga
     6001 tcctccaata gatcaacaac aacaggccag ttacgacagg cttctactac tgctgaaact
     6061 tctgagcagg gtcttaaaaa ttcagcaaat gactcattat tgttataaag ttttgctttt
     6121 tgtaaggcct gtcccttatg atctactaca gttactgtag agtagttttt gtgaaaatct
     6181 acgcctaagc aattcataat gcgcctcctt tagcgtcgat atacaaatca tatcttctcg
     6241 ttaaggcgca ctgtgtcttt atcatcatac ctaaacggaa tgagaaattt ttcttgacaa
     6301 gagaacatcc ttggggagat aatgggaata gaaaaggtta atggataaaa atgggattat
     6361 ataatgacca gcagtgttac aaggattccc gttatacacc tcgtatagag tgattatata
     6421 gaaattctgt aaagggggtc agagaacatg caacccgaag acttcaccta tgtgttttta
     6481 gcgcagcgcc cgcgaggccc tcgttgccgt gtggaagggg gccgaggaag ctggctgggt
     6541 catcgtaggt gattatgatc tctcccgtat ggtggcttcc ccgatagcaa attggaagta
     6601 aaaagtattg atatctgcaa gcccgacatg gcgcctccgt tcgctccatt ttcgaccctt
     6661 ctctgcccgt tttgagcact gcaacgcaaa atttaaggca aaagtgtaaa tttggttgtt
     6721 attgtagtaa caacatgaga atggtatgag cataatccaa gaattatctt agtcctcgaa
     6781 cctcttaaaa gccaaactcg cgttcgtccc accgaaccca aacgagttgc ttagggcagt
     6841 ttttatcttt aactctcttg cctcattcgg aacgtaatcc aaatcgcatt cagggtcagg
     6901 aaattcaaga tttatagttg gcggaataat cccattatac aaagcaagaa ctgtaaatgc
     6961 agcttcaatg cctcctgctg caccgagaag atgccctgtc attgattttg ttgaacttat
     7021 cgggagtcta taggcatagc tattaaaaac ctcttttaca gcgcgtgttt catttacatc
     7081 gttttgagga gttgatgttc cgtgggcatt gatataatca atatcctccg ggtttagctg
     7141 agcatctcgt agagcccttc tcatacacct aactgggcca tcaagcgaag gagatgtgat
     7201 gtgataagcg tcgctgctca ttccatatcc tataacttcg gcataaaccc ttgcacgatg
     7261 tctttttgca aactcgatct cctctaggat taaaatcccg gcgccttctc ccatcacaaa
     7321 cccgtcccgt tccttatcaa aaggcctaga ggcacgatga ggttcttcat tccttgttga
     7381 gagtgccctc attgcgttaa atccagctat tgcaactgga aggatgggcg cttccgttcc
     7441 tcccgcaatc atcacgtctg catcgcctct ttcaattatc tttgccgcat caccaatggc
     7501 atgggcgctc gccgcacacg ctgttgttgt acaactattc ggtccctttg cgtgaaagaa
     7561 tattgatacg tatcccgagg ccatgtttgc aatcattgaa ggaatgaaga aaggggatac
     7621 tcgagcaggc cccctttcat gcatctctat cacgtttttc tgaaatgaat caagcccccc
     7681 gataccagaa ctgacaaatg tccctacgtt tggagaaata tcctccgtga tttctagacc
     7741 cgcgtcattc aatgcgagct gtgtagcagc aacagcgtat tgaatgaatg gatcgcttct
     7801 tcttatctcc ttaggatcca taaaatcaac gggatctaaa tcaaggacct caccagctat
     7861 ctgagtcttg agcacagaag gatcgaactt ttgaatacgc cttatccccg gatatccctc
     7921 acagatcccc ttccagcttt tttcattccc cgtgcctagt ggggttataa gcccgacccc
     7981 agttacgact acccttcttt tcattacttc cgacgctcca agacatagtt tatagcatcc
     8041 tgaacggttg taatgcgctg cgcatcttca tccgagattt caaccccgaa ttcgtcctcc
     8101 atcgccataa cgagttctac caagtcaagt gaatctgctc ctaaatcctc gatgaatgcc
     8161 gattcgagag atatctcatc ctcagacttg ccgagctgac ttgcaatcat cttcctcacc
     8221 ttgccaacta attcctgatc atgttgaatt cccataatta attcctccca aatttttgtt
     8281 tttcaagtat aaagcccacc gtttaccctt acaacctccc ctgtaatata agatgcatca
     8341 tcagatgaaa gaaacgctac aacctttgca acatcctccg ggttgccaaa cctcccaata
     8401 ggaatacctt caacatattt attcctaatg ttctcaggaa gattacgtgt catatcggtc
     8461 tcgataaacc ctggactcac tgcattcact gttattcctc tggagccaag ttcctttgca
     8521 gcagatttag taaaccctat tataccagct tttgcggctg aatagttgac ttgtcccgaa
     8581 tttcccatct cccctatgat tgaactaata tttatgattc ttccatacct ctgcttcatc
     8641 ataatctttg ccgcagccct tgtacaatta aatacaccct ttaggtcaac ggaaagaacc
     8701 atatcccaat cttcttcttt catccttact ataagaccat cttttgtaat ccctgcgtta
     8761 tttactagta tatgaagtcc tccaaattgc tcccctatct tcccaatttc atcctgaact
     8821 acggttgtat tagcaacatc aaaatgccta atctcgcctt ttcctccaga attttctatc
     8881 gtaaatagta cttcccttgc cgcagcctca ttttgggcat agttaacgac aacgaataca
     8941 ccacgcgatg ctaactcaat tgctactgcc cttccaatgc ctcttgaagc acctgttaca
     9001 agcgctacct tatctttaaa ttctgccatt ttactcaagc ctaccagcaa gcctgcgtcg
     9061 agctcaggcg agacgacttg tggactaccc gatttttgtt ttatttcccc gtattgtgca
     9121 ggtcccgagt tctctcgagt gaactcggga caggcttgct cgcgtttaca ccaaacatat
     9181 cttacttctt aatctaaatg gttctccatc tttctttaag tgaattaagt tgttctattt
     9241 tttcaaaatt aaaaactgaa gcattagaaa cggtcctttt tattaaccca gaaagcacat
     9301 taccaggtcc gatctcaata tactctctaa cgccgttttc atataacata ttaacagatt
     9361 catcccatct aaccgggttt gcaacctggt ttaccaggag tttctttacc ttagatgaat
     9421 ccttatttgg tttcgcatca aaattcgata cgacagcggt ttgaagctcg tggattctga
     9481 cctggcttaa aacctcttct aatctcaaag cagcagagtt cattaacgca cagtgaaacg
     9541 gggcacttac ttcgagaagc aggacccttt ttgctccatt tgattttgct aaagcacagg
     9601 cttcctcaac cgcttcttta ttacctgaaa tcacaacctg accgggcgcg ttgaaattag
     9661 ctgggctaac tattaagtcg tctttatgag cctcaatgca aacttctcct accttctctt
     9721 tcgagagccc aagaatggca gccatagccc ctacgccatc aggaactgca gactgcatga
     9781 actcaccgcg ttttctaaca actagaacag catctttaaa atcaagacct ccgcttgcaa
     9841 ctagggcgga gtattcacca aggctgtgac cagcaagaaa ttctgcttta atacccattt
     9901 cctcatctag tactcgccat gcagcaacac ttaccgtaag ga
//
LOCUS       DATDRL010000238         2136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_181799, whole genome shotgun sequence.
ACCESSION   DATDRL010000238 DATDRL010000000
VERSION     DATDRL010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181799"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..247
                     /locus_tag="VI935_08115"
     CDS             53..247
                     /locus_tag="VI935_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25601.1"
                     /translation="MGKTESKPVLSFVEGLVLTIAINNRDIADNIPFGGITAVMRLLV
                     VGRNSGVNPKEGIIDDHCGN"
     gene            228..1127
                     /locus_tag="VI935_08120"
     CDS             228..1127
                     /locus_tag="VI935_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25602.1"
                     /translation="MTIAEIEGKISHTGQNLSERMEDLLTSDVFSACRYVRANTLLLP
                     FLRRAKGLNNNTLEHVLKEAVKEAHYLFWHRLRKTEPDVLIAIEFSSGYFFLVLVEAK
                     YFSSKSSSPWSEEELEEATAPRDQLAREYKELLDAHIVFHIPKSKVSGRALIYITVHR
                     SIPIDSVEESLKEIGKLVSGEDDINIFWASWFELHPITSQVENTLDWERPIVDDLRRL
                     LERKRLVHFRGFRLVVIKSISEGFVYKRKVEKRPVGYGFILAQETIKTHPIFYSSRPK
                     TREYCWAVPTKQLVGKIYNGGAL"
     gene            1124..1657
                     /locus_tag="VI935_08125"
     CDS             1124..1657
                     /locus_tag="VI935_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25603.1"
                     /translation="MSDQTDSIKTTFKNLITIYQETANLLLDAGSILEKAGYPCLHTN
                     TLGTEQSKNINNPSWWITPYASRYFATQENPAEIKAIGVFFVDKAYTPIEPLVLAGCF
                     KMKKNEKDEVLPLGYWYLKEAWFSLISDRKLREDLELEGNWNFSSAKIRAVPLDDIRD
                     QETLEKTIIAPLIAMSC"
     gene            complement(1833..2009)
                     /locus_tag="VI935_08130"
                     /pseudo
     CDS             complement(1833..2009)
                     /locus_tag="VI935_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367951.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
BASE COUNT          663 a          416 c          438 g          619 t
ORIGIN      
        1 aatttttaaa gaataatgag cctacttttt cattatttga agaagggggg aggtggggaa
       61 aacagaaagt aagcctgtcc tgagctttgt cgaaggattg gttttaacta tagcaataaa
      121 taaccgcgat attgcggata atataccatt tggagggata actgcagtaa tgcgattatt
      181 agtagttgga cgaaattctg gagtgaaccc aaaggaagga ataattgatg accattgcgg
      241 aaattgaggg aaagatcagt cacacaggac aaaacttatc cgagcgcatg gaagacttgc
      301 ttacttccga tgttttttct gcatgtcgtt atgtcagagc taacactctt ctgcttccat
      361 tcttacgccg ggctaaggga ttaaataaca acactttgga acacgtcttg aaggaggcag
      421 tcaaagaggc acattactta ttttggcatc gccttcgtaa aactgaaccg gacgttttaa
      481 ttgctataga gttttcttca ggatatttct ttcttgttct tgttgaggca aagtacttta
      541 gttcgaaatc aagttcacct tggagtgaag aagaacttga agaagctaca gcaccccgtg
      601 accaattagc tagagaatac aaagaacttt tagatgcaca catcgttttt catattccaa
      661 aatcaaaggt atcaggaaga gccctgatct atattacagt tcatagatct attccaatag
      721 attctgtaga agaatcgctt aaggaaatcg ggaaacttgt gtcaggggaa gatgatatta
      781 acattttctg ggcaagttgg tttgaattgc atccaataac ctctcaggta gaaaacactc
      841 tggattggga acgacctatc gttgatgact tgcgacgtct cctcgagcgg aaacgcttag
      901 ttcattttag aggatttaga ttagttgtga ttaaaagtat ttcagagggg tttgtatata
      961 aaaggaaagt cgaaaaaaga cccgtaggct atggattcat ccttgcccaa gaaaccatta
     1021 agactcatcc tatattctat tcatctcgcc ctaagactcg tgaatattgc tgggctgttc
     1081 caacaaaaca gttagttggc aaaatttaca atgggggtgc tctatgagtg accaaactga
     1141 tagtatcaaa acaacattca aaaatcttat aacaatctac caggaaactg caaacctttt
     1201 attagatgcc ggcagtatat tagagaaagc agggtaccct tgtctgcata ccaatacttt
     1261 ggggacggaa caatccaaaa atatcaataa tccctcatgg tggatcactc catatgcctc
     1321 acggtatttc gccacccaag aaaatcctgc tgaaatcaaa gcaattggag ttttctttgt
     1381 tgacaaagcg tataccccga tcgagccact tgttttagca ggttgtttca agatgaagaa
     1441 aaatgaaaaa gatgaggtct tacccttggg ctactggtat ctcaaggagg catggttttc
     1501 cttaatttcc gacagaaaac tgagagagga tttagagctt gagggtaatt ggaacttcag
     1561 ttcggcgaaa ataagagcag tcccgcttga tgacatcaga gatcaagaga cattagaaaa
     1621 aactatcatt gcacctttga ttgctatgtc atgttagaga tggtatcgta atccagataa
     1681 gcagaacttc gtctaatagc ggataaaaga gaaatcaaag attccctcaa atcccgacaa
     1741 gtcgggactt cttttattcg caaaacgtcg ttagccgcaa atatatgcct aatcggtttt
     1801 tttcagatta aaaaacaaag gaaggtttat acttatcccc tagccttcgc tgctctcttc
     1861 gcttcatctt ttaggagttt gtaatcgata ctatcaacca tcgctttcca gcttgcctcg
     1921 acgatgtttt ctgaaacacc gactgtgccc cagtcgtttt cgccgtcccc ggattctata
     1981 agtaccctta ctactgcatc tgttcctttc ctgttgaaac aacgcttact ccataatcct
     2041 ttaatttaac tggaaaggag gcttatcaga tatatgagag aaattcactt catttaagct
     2101 agaacacagg gcttcccaaa tatcggtatt gctatt
//
LOCUS       DATDRL010000239         9132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_542253, whole genome shotgun sequence.
ACCESSION   DATDRL010000239 DATDRL010000000
VERSION     DATDRL010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542253"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VI935_08135"
     CDS             complement(<1..259)
                     /locus_tag="VI935_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25604.1"
                     /translation="MYTLTSSQKDLLRRIVRQVREEKLPEEFSITWRSEGKGFVSVWK
                     GGSEEDIPTVTQGALDALAAAGLLLCDIEYYTAHSIKTELSR"
     gene            complement(430..1540)
                     /gene="trmFO"
                     /locus_tag="VI935_08140"
                     /pseudo
     CDS             complement(430..1540)
                     /gene="trmFO"
                     /locus_tag="VI935_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940494.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
     gene            2145..3905
                     /gene="pilB"
                     /locus_tag="VI935_08145"
     CDS             2145..3905
                     /gene="pilB"
                     /locus_tag="VI935_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HLE25605.1"
                     /translation="MEEKTKQSGSRFGDILVKQEVINPDQLKTAIEEQRRTGKRLGET
                     LLRLGYISQYELVAFLSKQYVVPAINLDEFDVMPEVLKFIPRESAVKHSLIPINRSGV
                     TLVVAMSDPSNIFAIDELKFATGHNIEVVVASEDSIKNAIERHYGDRENERARIHIEE
                     TGGSIDDIMGEIEYFIAELGKTEEMEVDDLAKASEEAPVIKLVNHILVNAIRHGASDI
                     HIEPYEKDYRVRYRIDGILYDTMRPPFKIKNAISSRLKIMSNLDIAERRLPQDGRIKL
                     RFGQRGTMEYRVSVIPTIFGEKVALRLLDRSSLQLDLTKLGFEEEQLTAFKDSIYKPY
                     GMVLLTGPTGSGKTTTLYSSLIELNRTECNISTAEDPIEYSLSGINQVQIHEEIGLTF
                     AACLRSFLRQDPDIILVGEIRDYETAEIGIKAALTGHLVLSTLHTNDAPSTITRLLNM
                     GVEPFLVTASLNSVVAQRLVRRICARCREEVNISPQVLIDLGVSPREVSEYKVYRGKG
                     DGCKACLGTGYKGRIAVYEVMVLTDELKEFVLSGASAIDLKREALRQGMKTLRQSALI
                     KLKHGITTVEEVVRNTASDN"
     gene            3917..5011
                     /locus_tag="VI935_08150"
     CDS             3917..5011
                     /locus_tag="VI935_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HLE25606.1"
                     /translation="MAATLHELLKVIVDSNASDLHITTGSPPMIRIDGKLVPIKHPPL
                     TPPETKDLCYSVLKDVQKQRFEENWELDFSFGLQGLSRFRGNIFMQKGAVAGAFRLIP
                     FNIRNLRELGVPPVVSELARKPRGLLLVTGPTGSGKTTTLAAIIDQINQERHEHIVTI
                     EDPIEYLLNHKNCLVNQREVGSDTKSFHDALRSVLREDPDVILIGEMRDLESIKIALV
                     LSETGHLTLATLHTNTAVQTINRVIDVFPPHEQSQIRAQLSFVLEGILCQTLIPKIGG
                     GRVLGVEVLVPNPAIRNLIREDKTHQIYSQQQIGQEKWAMQTLNQSLADLHLRKLITY
                     DDAILRSQDPEELKRLLSATNARIERAVRQ"
     gene            5068..6285
                     /locus_tag="VI935_08155"
     CDS             5068..6285
                     /locus_tag="VI935_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLE25607.1"
                     /translation="MAVYVWEGRLRGGKTQKGEMEAPNKSAVITRLRQMQIQPIPEKI
                     KEKGKLFRFNLQFGGVPTKDLVVFSRQLSTMIDAGLPLVKSLDTLIIQQNNVKFKNII
                     TNVKETVEGGSTFADALAKHPNVFNDLFVNLIRAGETGGVLDTILHRLSAYLEKMENL
                     KRRIRGAMVYPAVVIAVAIIVLSIIMIFVVPVFAELFKDLGTTLPALTQFIVNVSRFF
                     KNNIVYILIAVALLSFSIAAIYRRSKRGRKIIDAFLLKIWIVGDLILKTVLARFCRTL
                     ATLTAGGIPILDGLEITAKASGNKVTEEAILQVRKDIAEGKTLAEPLSENPKIFPPMT
                     VQMIAVGEQTGNLDGMLNKIADFYEEEVDVAVAALMSALEPVMIAILGGTVGVIVIAM
                     YLPMFKLVSTLAQ"
     gene            6309..7946
                     /locus_tag="VI935_08160"
     CDS             6309..7946
                     /locus_tag="VI935_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLE25608.1"
                     /translation="MNYSSDTDKGLKIFVVLRVIVITLLVGTGAFFYFKIGLRNEAML
                     LAFIIFIVYFLSFLYLLLYPLFQKFINLFKGIQISFDIIIASAVIYVTGGISSPFIFF
                     YVLIIIFANITLTRKAGYAAAASSATLYLIIVLYQLHQEHSFSIKEFPLSPIVLEDKE
                     LAYAFFKITGFLFIAMLSGYLSERMHLTRKELRESKESLVILENLHENILQNLISGVI
                     TLDLQKRLISINKTALEVLGIQSEDKVLGKELNNLIPTLSIEKLLSKRSEESLHSTND
                     GRNLTLEFSSSILKNSEGETGGYIIIFQDITEVKELEKRLRASEKMALMGQLAAGLAH
                     EIRNPLSAISGAIEVLSDEVKPTEDNTHLLNVVTQEVQRLNLLVEDFLLFARPIQKPE
                     VSVDISQVIDKTVELFSSATHGNGLQVVVNTEEELYVQVDPYRLKQVIWNLLLNAIQA
                     MPNGGKIVVEAHLEEGDVVIKVQDEGCGINEGVIPRIFEPFFTTKGGGTGLGLAIVKR
                     VVEGYDGRINVISSEDKGTAFIITLPKGEKLIQEIVH"
     gene            7969..>9132
                     /locus_tag="VI935_08165"
     CDS             7969..>9132
                     /locus_tag="VI935_08165"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLE25609.1"
                     /translation="MMQEKSKILVVDDEPGMRTFLEIILRKEGYSVETASDGMKALDN
                     INNNVFDLAILDMLMPVMNGIEVLKRIIEKSPETTVIIITAFASHETAIEAMKLGAYD
                     YITKPFKIDEIKLVIKKALDKKGLERENLRLRKELQTKYGFANIIGRSIDISKVFELI
                     KRVSELKVNVLITGESGTGKELVARAIHYSGSRHRGPFIAVNCGAIPEPLLESELFGY
                     RRGAFTGASKNKKGLFEEADGGTIFLDEIVDLPIHLQVKLLRVLEEKTIRPLGSTEPI
                     PVDIRIIAATNKLPEEEIANEKFREDLFYRLNVITIDLPPLRDRRDDIPPLAIHFVEK
                     YSREMGKDIHGISPKALETLESYHYPGNVRELENIIARCVALETSNVIRQQTLP"
BASE COUNT         2859 a         1707 c         1960 g         2606 t
ORIGIN      
        1 ttcgactcag ttcggtctta atagagtgag ctgtgtagta ctcaatatca cagaggagca
       61 aaccagcagc agctaatgcg tctaatgcac cctgtgttac tgtcgggata tcctcttcac
      121 ttccgccttt ccacactgaa acgaaacctt ttccctctga tcgccaagtt atggaaaact
      181 cttccggcaa cttttcttca cgaacttgcc gcacaatccg tcgtagcaaa tctttttgac
      241 tacttgtgag ggtatacatg tttgttaact tacaagggca tactagaagc tgccttctat
      301 gattattcgg aacttttccg tataatcgcc gggatattgt gcagaaatct ttgcaccatt
      361 tttcagtgtt agagaataaa ttaaaatatc tctccggggc aagaggcgta aatcaagcga
      421 gtaaaaatat caaaacggat tcagctcaga tattctagca agtgctcttt ctacgataag
      481 ctttctcctc tgtgattttg ggacgccatt tggaagagga ggaaatagcc caaagtttat
      541 gttcataggc tgaaaattct ttacaccctg gtcattgata tactttataa gcgcaccaat
      601 tgcagtttca ggcgagggta tgttagcttt gtctgaaatc ctaccatgtt atacatagtg
      661 ccgtctttgt tttccatcct aagctgaacc actgaaaaag gctttttccc agtacgtggg
      721 tcggtaagcc ctataggctt cacgggacca aacctcgatg tatctcgtcc cctttccgcc
      781 atttcctcta ttggaaggca actttcgaag tatagcgccc tttcaacatc cctagtctcg
      841 accctatctg agtttaggat ctcttatatg aatctgtaat attcatcctc gcctaaagga
      901 cagtttaaat aatctccctc ttcgggatct gggtagtgat cctgtgggag ggcagtcggg
      961 gaaccacttc ctctcccttt ccatatctcg aagcacgaaa gaccttgccg cagtctatag
     1021 tctcagcatc tattaaaggg gagatagcat cgtaaaaata aagatgagag gagcccgtaa
     1081 ttcttctaat ttcctctgat agtgattcag aagttagagg accggtaaca attattatag
     1141 aacctcttac aggtgaagga atctctttaa cctcttctct tacaatttct acaagtgggt
     1201 tttcttcaat catttttgtc aggtactgag aaaactcaac tcgatcaaca gcaagcgcct
     1261 ttcctgcagg aacaattgta ttatctgccg cttcgataac tatggagact agttttcgca
     1321 tttcctcctt cgatataccg ctcgcatttt caagcgagtt ggataaaagt gagttactac
     1381 acacaacctc ccctagataa tctgttttat gagccggtgt aggattttga gatctcatct
     1441 catagaggtt taccttgagg ccaaattttg taacctgata tgctgactca gttcccgtta
     1501 agccatcccc aattattgtg actactcttt ctttagacac gagattaaaa ttctacccaa
     1561 tgacaattct gactcaattc agcattcagt tagcaagatg gaatatgtgt tcctaactca
     1621 ctaagcttct tatggattca aacccatttt atacaatcca actttgtaat cggaattcaa
     1681 aattaatgat cccaccattt tttatattat cctagtcctc aaaacaaagt tttaatcaat
     1741 ttgcaatgag attaaaacgg ctattttaca aatttattgt gttttcaata agttatattc
     1801 tagctttaat atttctgatg ctttttgtaa agagttatgt tattgtaaaa taagattaaa
     1861 acaagatgga ttggctttaa aatacgaaat gttttaaatt ctacaacaat gggttcagaa
     1921 acccttcaaa atatgggttc agaaaccctt caaaatatgg ttactatcct attatgtcag
     1981 gcttagaaga tatagaggaa agtcagaagg aattggatta ttcagtttgg ataaactcta
     2041 ccaaatatct gttaaaagct gtgggtatca tttattatga aaagtaagta aaattgttga
     2101 ttattgtatt aatttactaa atgttatata taaggatgga ataaatggaa gaaaaaacaa
     2161 aacagtcagg aagtcgcttt ggcgatatat tggtaaaaca agaggttatt aatccagatc
     2221 aactaaaaac cgctatcgag gaacagagac gtaccggaaa aaggcttggt gaaaccctcc
     2281 ttaggttggg ttacataagt caatatgagc tagttgcatt tctaagtaag caatacgtag
     2341 ttccagccat taatctcgat gaattcgatg taatgccaga ggttttaaaa tttatcccac
     2401 gagaatctgc agttaagcac tcccttattc ccataaatcg gtcaggggta acactagttg
     2461 tggctatgtc tgacccatcc aatatatttg ctatcgatga gctaaaattt gcgactggac
     2521 acaacattga agtggtagtt gcttcagaag attcaattaa aaacgcaatc gagaggcatt
     2581 atggagacag ggagaatgag agggcgagga tacatataga agaaactggg ggatcaatcg
     2641 atgatataat gggagagatt gagtatttca ttgcagagct aggtaagacg gaagagatgg
     2701 aggttgatga cttagccaag gcgtcagaag aagcacctgt tattaaactt gtaaaccaca
     2761 tccttgttaa tgcaataaga cacggtgcaa gtgatattca catcgagcca tatgaaaagg
     2821 actatagggt gagatatcga attgatggaa tcctttacga cactatgcga cctcctttta
     2881 agataaaaaa cgcgatctct tctaggttga agattatgtc gaacctagat attgctgaga
     2941 gaagacttcc tcaggacggt agaataaaat taaggttcgg tcaaagggga actatggaat
     3001 atcgcgtctc agtcattccg acgatttttg gggaaaaggt cgcgctcagg cttcttgata
     3061 gatcatccct gcaactcgac ttaactaaac ttggttttga agaagaacag cttacggcgt
     3121 tcaaggatag tatttataaa ccctatggca tggttttgtt aacgggtcct acaggaagtg
     3181 gaaaaacaac tacactctat tcaagcctta tagagctaaa tcgtacggag tgtaatatct
     3241 ctacggctga agaccctatt gaatatagcc tttcaggaat aaatcaggtt cagatacatg
     3301 aagagattgg gcttactttt gctgcatgcc ttagatcgtt tttaaggcaa gatccagata
     3361 taattctcgt tggagaaatt agggattatg aaacagcgga gatcggtatt aaagcggccc
     3421 tcactggtca cctagttctt tcgacactcc acacaaatga cgctccttca actattacga
     3481 ggctacttaa catgggcgtt gagccatttc tagttactgc gtctttgaac tcggtagtcg
     3541 cacaaaggct tgtaagacgg atttgtgcac gttgtagaga agaggtaaac atttcgcctc
     3601 aggttttgat agacttaggg gtatcaccga gggaggtatc cgaatataag gtttatagag
     3661 gtaaaggcga cggttgcaaa gcatgccttg gtaccggata caaaggaaga atagccgtat
     3721 atgaagttat ggtactaacc gatgagttaa aagagtttgt tttaagtgga gcgtctgcaa
     3781 ttgatcttaa aagagaagct ttaaggcagg gaatgaaaac acttaggcag agtgccctta
     3841 tcaaacttaa acatggcata acgacagtcg aggaggtagt aagaaataca gcatccgata
     3901 attaggaggt attaatatgg ccgcaacact tcatgagctt ttaaaggtaa ttgtagatag
     3961 taacgcctca gatcttcaca ttactacagg ctctccaccg atgatcagga ttgatggaaa
     4021 gctcgttcct attaagcacc ccccgcttac tccacctgaa accaaggatt tgtgctatag
     4081 tgtccttaag gatgtgcaga aacagaggtt tgaagaaaac tgggagcttg acttttcatt
     4141 tggcctccag ggactaagcc gtttccgtgg caatatcttt atgcaaaaag gggctgtcgc
     4201 tggggctttt cgactgattc cgtttaacat aaggaatctt agggagcttg gagttccacc
     4261 tgttgtttca gagctagcaa gaaaaccaag gggtctcttg ctcgtaactg gccctacagg
     4321 ttctggaaag actactaccc ttgcagctat aatagaccag attaaccaag aacgccatga
     4381 acacatagtc acgattgaag accctattga atacctttta aaccataaga actgccttgt
     4441 aaatcagaga gaggtaggaa gcgataccaa gagctttcat gatgctttaa gaagcgttct
     4501 cagggaagac ccggatgtga ttttaatcgg ggaaatgaga gaccttgaaa gcatcaagat
     4561 agccctggtt ctttcagaaa cgggacattt aacacttgca acacttcaca caaatacagc
     4621 tgttcaaaca ataaaccgtg taattgacgt ttttcctccg cacgaacagt ctcaaattag
     4681 ggcacaactg tcttttgttt tggagggaat attatgtcag acacttatac ctaagattgg
     4741 gggggggagg gtgctcggtg tagaggtgct tgttcccaat cccgctatta ggaaccttat
     4801 cagggaggat aaaacgcatc agatttattc acagcagcag ataggtcagg aaaaatgggc
     4861 gatgcagacc ctcaaccaga gtcttgccga tttgcatttg agaaaactta taacttatga
     4921 tgatgcaata ttgagaagcc aggacccaga ggaattaaaa agactgcttt cagcaactaa
     4981 tgccaggata gagagggcag ttagacaata gctatgaact ttagatgtca gattaatctt
     5041 taaatgtaaa acaaggaggg tagaaagatg gcggtatatg tttgggaagg caggttaaga
     5101 ggcggtaaaa cccaaaaggg ggaaatggaa gctcctaata aatctgcagt gataacccgc
     5161 ctgaggcaaa tgcagatcca gcctatacct gaaaagatta aagaaaaggg aaaacttttc
     5221 aggtttaacc tccaattcgg aggtgttcct actaaagatc tcgtagtatt ttcaaggcag
     5281 ttatccacta tgatcgatgc aggtctccct ctcgttaagt cacttgatac attaattatt
     5341 caacagaaca atgttaagtt taaaaatata ataaccaacg taaaagaaac ggtagaaggg
     5401 ggctcaacct ttgcagatgc ccttgccaaa caccctaacg tttttaacga cctctttgta
     5461 aatctaataa gggctgggga gacaggcggt gttcttgata cgattctcca tagactctcg
     5521 gcatacttag agaaaatgga gaatttaaag agacgcatca ggggggcaat ggtttatccc
     5581 gctgttgtaa tagctgtggc aattatagtc ctctcaataa taatgatctt tgttgtccct
     5641 gtttttgcag agctctttaa ggacctaggc accacacttc ctgctctcac tcaatttatt
     5701 gtgaacgtga gtaggttttt taaaaataat attgtttaca ttcttatagc cgtagcctta
     5761 ttatcattca gtatcgctgc aatttataga cgttctaagc gaggaagaaa gatcatcgat
     5821 gccttccttc ttaaaatctg gatagtagga gacctaattc ttaaaaccgt tcttgctagg
     5881 ttctgcagaa cacttgctac acttacagca ggcggcattc cgatattaga cggactagaa
     5941 attactgcaa aagcatccgg caataaagtt acagaagaag ctattctaca ggtaagaaag
     6001 gacatagccg aggggaaaac acttgccgag ccactatcag aaaatcccaa gatattcccg
     6061 cctatgacgg tgcagatgat tgccgttggt gagcaaacag gaaatttaga tggtatgctc
     6121 aacaagattg ctgactttta cgaggaagaa gttgatgtcg ccgtcgcagc cttaatgtca
     6181 gcactcgaac cggtaatgat tgctattcta ggcggaactg taggggtgat agtgatcgct
     6241 atgtatcttc ccatgtttaa gcttgtatcc acgcttgctc aataatactt ttattatcac
     6301 tgagcagaat gaactatagc agtgacactg ataaaggact caagatcttt gtagttttaa
     6361 gggttatagt tattaccctt cttgtcggta caggggcttt tttttatttt aaaattgggt
     6421 tgagaaatga agccatgctt ttggctttca tcatctttat tgtttatttc cttagttttc
     6481 tttatttgct tctgtatcct ctttttcaaa aatttattaa cttatttaaa ggaatccaga
     6541 tttcttttga tataatcata gcctcagcag tgatttatgt cactggtgga atatcaagcc
     6601 catttatctt tttctacgtc cttatcataa tatttgctaa tataacgctc acaagaaagg
     6661 caggttatgc cgctgctgcg tcatcagcca ctttgtatct tataatcgtg ttatatcagc
     6721 ttcatcagga gcattccttt agtataaaag aatttcccct atcccctata gtattggagg
     6781 ataaagagtt agcttatgct ttttttaaaa taacgggttt tctttttata gcaatgctta
     6841 gcggatactt atctgaaaga atgcacttaa ctcgaaaaga gcttagagaa agcaaagaga
     6901 gtcttgtaat tctcgagaac cttcatgaaa acattcttca gaatcttata agcggcgtta
     6961 taaccctaga tcttcaaaaa aggcttatat caataaataa aacagcactg gaggttcttg
     7021 gaattcagag tgaagataag gtattgggca aagagttgaa taatcttata ccgacgctta
     7081 gcatcgagaa actattatca aaaagaagcg aagagagcct acattcaaca aacgacggaa
     7141 gaaatcttac cctcgaattt tcttcatcta ttcttaaaaa ctcagaagga gagactggag
     7201 gatatattat aatctttcag gatattacag aagttaagga gcttgaaaag cgtcttagag
     7261 catccgaaaa gatggctctt atgggtcaac ttgcagctgg gctagctcac gaaataagaa
     7321 atcccctctc agctataagc ggggcgattg aagttctaag tgacgaggtt aagccaactg
     7381 aagacaatac tcatcttctc aatgttgtca cccaggaggt tcaaagactc aaccttcttg
     7441 ttgaggattt tctccttttt gcaaggccga ttcagaaacc ggaggtttca gtggatataa
     7501 gtcaagtaat tgataaaact gtagaattgt tctcaagtgc cacgcatgga aatggccttc
     7561 aagtagtcgt taatactgag gaagagctat acgtacaagt agacccatac aggctaaaac
     7621 aagttatctg gaatcttctc cttaacgcga ttcaggcaat gccaaatggc ggaaaaatag
     7681 tagttgaagc tcatctcgaa gaaggggatg ttgtgattaa ggttcaagat gagggatgcg
     7741 gtataaacga aggggttatt cctagaatat ttgagccctt ttttaccaca aaggggggag
     7801 gaacagggct tggtcttgca attgtaaaga gggttgtaga aggctatgat ggaaggataa
     7861 atgtcataag ttctgaagac aaaggaacgg cttttattat aacccttcct aagggtgaaa
     7921 aattaataca ggaaattgtg cactgagaga ttaaattcga gatagcatat gatgcaggag
     7981 aaaagcaaaa tacttgtcgt agacgatgag cccggaatga ggacgtttct agaaatcatt
     8041 cttaggaaag agggctacag tgtagagacc gcgtctgacg gtatgaaggc tctagataat
     8101 ataaacaata atgtattcga cttagccata ctagacatgc tgatgcccgt tatgaatggg
     8161 atcgaggttt taaagaggat catagaaaaa agccctgaga cgaccgtgat aattataacc
     8221 gcgtttgctt cacatgaaac tgcaattgag gctatgaagc taggagcgta tgattacatc
     8281 actaaaccct ttaaaattga tgagataaag cttgttataa aaaaagcact cgataaaaag
     8341 ggactcgagc gtgagaattt aaggctcaga aaagaattgc aaacaaaata tgggtttgca
     8401 aatataatcg gaagaagcat agacatatca aaggtttttg agttaataaa aagggtttca
     8461 gagcttaaag taaatgtgct tattacagga gaaagcggaa caggtaaaga gcttgtagcc
     8521 cgcgcgatac actatagtgg atcaaggcac cgaggcccct ttatagccgt aaactgtggg
     8581 gcaattcctg aacctctttt ggaaagtgag ctttttggtt ataggcgagg ggcttttaca
     8641 ggagctagta agaataaaaa aggacttttt gaggaagcag atggcggtac gatcttctta
     8701 gatgagattg tagacttgcc aatccatctc caggtaaagc ttcttagggt gcttgaggaa
     8761 aaaacaattc gtccgcttgg aagtacagag ccgatcccgg ttgatattag aataatagca
     8821 gcaacgaata aactccctga agaggaaatt gcgaatgaga agtttcgtga ggaccttttt
     8881 tatcgtctaa atgtaataac aatcgatctt cctccactca gagatagaag agatgatata
     8941 cctcctctag caatacattt tgttgaaaaa tattctcgcg agatgggaaa ggacattcat
     9001 ggtatttctc ctaaggcatt agaaaccctt gagagctatc attatcctgg aaatgtaaga
     9061 gagcttgaaa atataattgc aaggtgcgtc gcgcttgaga catctaatgt gatacgtcaa
     9121 caaacgcttc ca
//
LOCUS       DATDRL010000240         3274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2215674, whole genome shotgun sequence.
ACCESSION   DATDRL010000240 DATDRL010000000
VERSION     DATDRL010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2215674"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VI935_08170"
     CDS             <1..961
                     /locus_tag="VI935_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HLE25610.1"
                     /translation="LIEFKGIANKLNFYSDVILSFLDSSEDGYVRWVEGNIGRGGVFS
                     GIGLSPLDISPQLKEKLYSRCRTVVMTSATLSVKRSFDFIKSGLGLEDNERISEIILP
                     SPFNYREQAFLAIPRDIPEPVQKGYSDKLSPIILEAVRISRGNALILFTSYSLLETVY
                     EKIKKELSGLGIISLKQGSLPRARLLNKFKVEDNSVLFATDSFWEGVDVPGEALRLVV
                     ITRLPFRVPTDPIIEARVEHMEKQGLNSFLEYTVPVAVLKFKQGFGRLIRTKNDRGSV
                     LVLDKRVISKHYGRHFLDSLPKCKLVMGSTHEVIKELESFFGD"
     gene            1298..2542
                     /locus_tag="VI935_08175"
     CDS             1298..2542
                     /locus_tag="VI935_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25611.1"
                     /translation="MLPHKSLIKFFAVVVATVSKGGASAISAVWTFPAVIFSAMLIAW
                     AAEAAQFLISQGLALAILAWIQTLPEFAVEGVIAWSAGKDPSKIHLVTANFTGAIRLL
                     PGLGWPLIFFTAFIFNKIKTGENIKEIVLKKEHSLEVLALLPPLIYFVVIFLKGTFNI
                     IDSLVLILLYSSYLYSLNKLPPQEVEGVDELEPIPRSILRRKPLFRNSIIMGLFLAGG
                     LILLFVAQPFLHSMLALAVTLGISEFVFVQWVAPFLSEFPEKVSAFYWARQVKKASIA
                     LMNMVSSNINEWTMLAAMIPIVFSLSAGGVSTIHFDEFQLTEILLTIVQSLLAFMLLL
                     NMKYSWYEALGLFLLWAIQFAQPHLREEIIFVYLAWIAFCLIQILFGWREPQAIKDFM
                     HILRSYILVSKPSTQIDPEERK"
BASE COUNT          909 a          627 c          707 g         1031 t
ORIGIN      
        1 tttaattgag tttaaaggga ttgcaaataa gctaaacttc tattcggatg tgattctttc
       61 ctttctcgat tcgagcgagg atggatatgt tagatgggtc gaaggcaata ttggacgggg
      121 aggggttttc tcaggaatag gactttcacc tcttgatatt tctcctcagt tgaaagaaaa
      181 actgtattct agatgtagaa cagttgtgat gacctctgcc actttatcag taaaaaggag
      241 ctttgatttt ataaaatctg ggcttggtct tgaagataac gagagaataa gtgagattat
      301 tcttccatct ccctttaact atagggaaca agcatttctt gctattccta gagatattcc
      361 cgagcctgta caaaagggat attccgacaa attatctcca atcatactcg aggctgtaag
      421 gatatcccgt ggaaatgcac tgattctttt cacatcttat tctcttctag aaacggttta
      481 tgaaaagatt aaaaaggaac tttcaggact cggaattatc tctctaaaac aggggagtct
      541 tcctagggcg aggcttctta ataagttcaa agtagaggat aattccgttc tttttgcaac
      601 cgatagcttt tgggaaggtg ttgatgtccc tggggaagcc ttgaggcttg ttgtaattac
      661 aaggctgcca ttcagggttc ctacagaccc tataatagaa gcaagggttg agcatatgga
      721 aaagcaagga ttaaattcct ttcttgaata taccgttccg gttgcagttc ttaaattcaa
      781 acagggtttt ggaaggctca taagaacgaa gaatgatagg ggttcggttt tagtcctaga
      841 taaaagagta ataagcaagc attacggaag acatttctta gattctcttc caaaatgtaa
      901 gttggtcatg ggttctacac atgaggtaat aaaagagctc gaatcatttt ttggagactg
      961 agctgggttc gtggctcgtg attcgttgtt cgtggttagc cagatcgaga ccttagtctg
     1021 agcctggctt catcgtgagc tcggcagaac ggggggcggg gttggaaaac cccgcctatc
     1081 cttatgtatt tgcgcgttat ttctttttga tgtagaagaa acctttaggt tttcaatata
     1141 attattaatg gaaggctaaa gccttacgct acattttcta ttctaagaag tcgattcacg
     1201 aatcacgaga cccgaataac gaaaaataaa ttgacagtta taacccccta agatattttt
     1261 attcaaccct tctcacaaac cgaggcaaga aaaatctgtg ttaccccata aatcgctcat
     1321 aaagtttttt gcggtagttg tagctactgt gtccaaaggc ggggctagcg caatctctgc
     1381 cgtctggaca ttccccgctg taattttttc tgcaatgctc attgcgtggg cggctgaggc
     1441 tgctcagttt cttatctctc aggggctcgc ccttgcaata cttgcatgga ttcaaacgct
     1501 tcctgaattt gcagtcgagg gggttatcgc atggtctgct ggaaaggacc caagtaaaat
     1561 ccatcttgtt actgcaaact ttacaggtgc aatacgtctt cttccgggtc ttggctggcc
     1621 ccttattttc tttacagcct ttatttttaa taaaattaaa actggagaga atataaaaga
     1681 gatagttctg aaaaaggaac attccctaga agtccttgct cttcttcctc ctcttattta
     1741 ctttgttgtg atatttctta agggtacttt taatataatt gatagcttag tgcttattct
     1801 actctattcc tcatatcttt actccttgaa taaacttcct cctcaggaag ttgaaggtgt
     1861 agatgaactt gagcctatac ctagaagtat ccttcgtcgt aaaccgcttt ttagaaattc
     1921 tataattatg gggctatttc ttgcaggtgg tttaatctta ctctttgttg ctcagccttt
     1981 tcttcatagt atgcttgccc tggctgttac tttaggaatc tctgaatttg tatttgttca
     2041 gtgggttgcc ccttttctat ccgaatttcc agagaaggtt agtgcatttt attgggcaag
     2101 gcaggtgaaa aaggcatcta tagccctgat gaacatggtt tcatctaaca ttaacgaatg
     2161 gactatgctt gccgcaatga ttccaattgt gttcagcctg agtgctggag gtgtctcaac
     2221 aatacatttt gacgaattcc agcttaccga aatccttctt actatagttc aatcacttct
     2281 tgcgtttatg cttcttctta acatgaaata tagctggtat gaggctctgg gactttttct
     2341 tctctgggca attcaattcg cacaaccgca cctgagagag gaaataatat ttgtgtatct
     2401 tgcttggata gccttctgcc ttattcaaat actatttggc tggagagagc ctcaagctat
     2461 taaggatttc atgcatattc ttaggagtta tatattggtc tcaaaaccgt ctactcaaat
     2521 agacccagag gaaagaaaat agtaactata cagccacttt ctttgacact aagtcacccc
     2581 tcatataaac tttataggta tcttttctat ggattttata ccaattcgat ttttcgatag
     2641 tgcacacgga agtctgtcat tgcgagaaga attatttcga gcgccgcttt atttgaacca
     2701 gccttatctc tatcgcagtg ggatagagat gggaataaac agggtggttc aaagggaaat
     2761 acaaagaacg agaaatagaa ataaaaggca gtgacgaagc aatcttttat tatctatgac
     2821 attgcttcgc aacactcaac aatgacagga aagactgtca ttccgaatac ttcggatggg
     2881 ctcagcacat gctccgtgag gaatctagat ctctcacgta cgttcgagat gacaactagg
     2941 gtcagattgc ttcacggagt ttacactgag tttaacgaag tgttcgcaat gacaattgga
     3001 agcaatcttt tcgctgggat ctggggtatc cccgatcaag tcggggataa aagagggagg
     3061 gtagttaggg aaccgtaacg ctcgcgatgg cgactttttt tgaatgcaat aacacttgat
     3121 agaattaaat tttaggaggt gtgtatttaa atgcctactg cttcacaaat agcgcctgta
     3181 aacatcgaag atgagatgaa ggaatcatat ctcagctatt cgatgagcgt cataattggg
     3241 cgcgccctgc ccgatgtaag ggatggtcta aagc
//
LOCUS       DATDRL010000241        13807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3255823, whole genome shotgun sequence.
ACCESSION   DATDRL010000241 DATDRL010000000
VERSION     DATDRL010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3255823"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VI935_08180"
     CDS             complement(<1..663)
                     /locus_tag="VI935_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25612.1"
                     /translation="MPKHKMETIVITISNPKELTQGTSDSFFIPLVRPLRRVLENGVI
                     GRITYVLYQELNKPLRTFGAFCYTPGERLLFFPGIVKRSLRWNSEGKQKIEKFNLPTD
                     TLIDHFSLEKELDKWHITLLDSNGEKKYPLRKYKTRRYQDNSLFWFGLSVKSPYVLDP
                     TPRESKFIFPSPETDSERRIQDIIKARDGAVFSLISLPNGLTLNTDEYLHFEVLVTKE
                     KRD"
     gene            995..1312
                     /locus_tag="VI935_08185"
     CDS             995..1312
                     /locus_tag="VI935_08185"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLE25613.1"
                     /translation="MKEIDKILPNIKKALKTIYGDQLLEVILFGSCARGTFTKDSDID
                     IAVVLKGRVNRTKEIDRIYDTLYELILETGELISIYPVSEEEILNSVWPLYHHIRNEG
                     IRI"
     gene            1332..1700
                     /locus_tag="VI935_08190"
     CDS             1332..1700
                     /locus_tag="VI935_08190"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HLE25614.1"
                     /translation="MQRVARAIKSVELLLKEGDFDSSVSRSYYAIFYATEAVLLTKEL
                     KFSSHKSVITLFGEHFLKTGTFKPEMGRRLSKTFEKRLIGDYSFEPQVTMKDTKETLN
                     WAREFVKDIRNYLIKEEYMK"
     gene            2044..2823
                     /locus_tag="VI935_08195"
     CDS             2044..2823
                     /locus_tag="VI935_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HLE25615.1"
                     /translation="MESNSSEVIKESKDPSSECEKASMLEDRYFLEGPRSRIQELFFT
                     IRVVIEFIKGFRVLHFVGPCVTVFGSARLKEDHPYYQLARNAGGRLVKLGFTVMTGGG
                     PGVMEAANRGAKEAGGMSVGCNITLPVEQKPNPYLDKWVTFNHFFVRKVLLVKYSYAF
                     VVMPGGVGTLDELFEVITLIQTKKILDFPVVLIGKEYFQDAMEFFQRMVLEGTIGISD
                     LNLILLTDSLDEAISHIEKHAVEKFKLRRRKVPKPSRLLRE"
     gene            complement(3005..3080)
                     /locus_tag="VI935_08200"
     tRNA            complement(3005..3080)
                     /locus_tag="VI935_08200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3045..3047),aa:Arg,seq:ccg)
     gene            3294..3659
                     /locus_tag="VI935_08205"
     CDS             3294..3659
                     /locus_tag="VI935_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLE25616.1"
                     /translation="MANMQKKSVNSPDETRSFPKGKLELVTVAGITFGRATLEPGWKW
                     SECVKPIAKTKSCEAPHTQYHVSGRLRVRMDDGTEQEFGPGDVSHLPPGHDGWVVGNE
                     PVVVIDITGMTHFAEQAKK"
     gene            3864..4283
                     /locus_tag="VI935_08210"
     CDS             3864..4283
                     /locus_tag="VI935_08210"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HLE25617.1"
                     /translation="MITIIKTVFLGLVLIVASNITAHAEDWVFITLSPDGEFALYIDK
                     DSLVFSDNKTWYWSKLEFAEPQESVKEGEIYSILHSFIEIDCAKKTFTNPYAIEKKKD
                     GQVVWRGHIPARPDIPIPPDSIIEREFEFVCIKLIPR"
     gene            complement(4779..5543)
                     /locus_tag="VI935_08215"
     CDS             complement(4779..5543)
                     /locus_tag="VI935_08215"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HLE25618.1"
                     /translation="MSLELELKELLEELKRYFECQKILGLDTIALAREKKNEESSETY
                     RKELKYSVLTEKRTRIYIEEQLGIFDKPSKKLTLEEIREEIGDCTRCKLHQGRTNIVF
                     GEGNPKARLVFVGEGPGEEEDKQGRPFVGRAGQLLTKIIIAMGLRRSDVYICNVVKCR
                     PPGNRSPEPDEAGTCEQYLFKQLRAIDPDIIVCLGSVAAQSVLRIKEKLGNLRGKFHS
                     YGRAKVMVTYHPAALLRNPGYKKPLWDDMQIVIKEL"
     gene            6216..7139
                     /locus_tag="VI935_08220"
     CDS             6216..7139
                     /locus_tag="VI935_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLE25619.1"
                     /translation="MKILVTGGAGFIGSWVADTFINKRHKVLIVDDLSTGIEANIPKD
                     AEFTKGNIREFALLEKVFSNFKPDIVNHHAAQINVRRSLEDPVFDAEVNIIGTLNLLE
                     LSVKHNIKRFVFASSGGAIYGEPENLPADENTCPIPISPYGVSKHAIEKYLGYYKTVH
                     RLDYVALRYSNVYGPRQNPHGEAGVLAIFCNRIMAGKPCEIFGDGNQTRDYVFVGDVT
                     NANILSINSPSGIYNIGTQIETSVNDLITTLRKVTKREFKVEYSPPRAGEVLRIYLDI
                     QFAKRVLGWSPNVNLEDGIHQTWKWFNDRDS"
     gene            7215..8201
                     /locus_tag="VI935_08225"
     CDS             7215..8201
                     /locus_tag="VI935_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HLE25620.1"
                     /translation="MDFSELTRISSGFSHSRILQVALKLGVFDVIKRQGGSSVEVAKA
                     LGTNPRATELFLNALVALRFLRKKEGKFYNTNTSLKYLAKDSPKFFGGMILFEEGLWN
                     MWAKLEESIRTGKPARATDMFQKSEEETERFIMAMHSLVSARGDAEILSNKLDFSWAQ
                     TMIDIGSGPGSYPIKFLQRYPHLKITVLDLHGTLKVTKQVLKKEGMNRKIKLLECDYN
                     SDEIPKGFDVAFLSNIIHSEDEESNEKLLKKIYESLNHGGEIIIKDHILNHTLTSPAV
                     GAIFSIQMLLTTKGRDYSFREVRGWLMDVGFKKVKWIRLEPPLTSSLVIGWK"
     gene            8316..8516
                     /locus_tag="VI935_08230"
     CDS             8316..8516
                     /locus_tag="VI935_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25621.1"
                     /translation="MMRRYEIYFDVRVIERDIREGTITKKDYEEYLKKLSDISEKGCT
                     LVIDEATNQPSEITNSESGENG"
     gene            8513..8719
                     /locus_tag="VI935_08235"
     CDS             8513..8719
                     /locus_tag="VI935_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25622.1"
                     /translation="MKWDYRVIPIDELFPEHDDHDIAVSKQAATTRRAKIGSGFEQNL
                     HKLGNEGWEFVNIVGEFGIFKKPS"
     gene            8927..10000
                     /locus_tag="VI935_08240"
     CDS             8927..10000
                     /locus_tag="VI935_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25623.1"
                     /translation="MNLTCVRSGVNNIFYLFALFLILTLPNSAFSVTVSVEGFEPLSG
                     KTIASARTMALSNALKTAVENAAKGINPTLDAKSLEKLINENPITYIKAYRILDENRI
                     GDDYKIILEANVDSEMLKDKIKGLKVNNLKSPGKPSISIVVLKIPESDPIIKSLPLSE
                     IKSEITRVLQGGGYKVQESMGDIRIETYVSVKTSESKVGRSMCYALGNVLIKVKDKNE
                     KVLNEVSDSLYLNGTDITQLSLEAIKQAGAKAADKVKLEFDKKWNIGQRVKSKTILIT
                     FVGFSNYKQYELLDTILANSTPGIDNITKRIFRNDGVSFLVLSSISEEDLAKTVDSQN
                     TPGFSLRLNEVSSDRIEFSVVTE"
     gene            complement(10084..11616)
                     /gene="guaA"
                     /locus_tag="VI935_08245"
     CDS             complement(10084..11616)
                     /gene="guaA"
                     /locus_tag="VI935_08245"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HLE25624.1"
                     /translation="MRETVLVLDYGSQYTQLIARRVRELGVYSEIKPFNEPLERIKET
                     QPRAIILSGGPASVWDEGAPSLDPGLFNLGVPILGICYGMQLIAHLLGGKVERSTRRE
                     FGPAEIEVTDTLDIFSNIPRMTRVWMSHGDRVLELPKGFVSIAMTENSPVAAMKHTGG
                     RIFGTQFHPEVIHTELGKEILSNFVIKISKCQGSWTAKSFAGTAIKEIRQKVGNSHVV
                     CALSGGVDSAVTALIVQRAIWDKLHCIFVDTGLLRLNEAKQVVETFKDLGLNLTHVNA
                     RRRFLDKLRGIEDPEMKRKIIGEEFIKVFEDEASKIEGAEFLAQGTLYPDVIESVSVK
                     GPSAKIKTHHNVGGLPERMNLKLIEPLRELFKDEVRAVGRELGLPSEILDRHPFPGPG
                     LVIRIMGEITEEKLEKLRYADAIFIEEIKRAGIYNDIWQAFCVLLPVKTVGVMGDERT
                     YENVIALRAVTSLDGMTANWARIPFEILEKISARIINEVKGINRVVYDISTKPPSTIE
                     WE"
     gene            complement(11909..13375)
                     /gene="guaB"
                     /locus_tag="VI935_08250"
     CDS             complement(11909..13375)
                     /gene="guaB"
                     /locus_tag="VI935_08250"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HLE25625.1"
                     /translation="MFENKMEEFLTFDDVLLLPDYTGVLPTEVDVSTYLTTEIKLHIP
                     LISAAMDTVTESKTAIAMAQEGGIGVIHRNLTLAEQVAEVEKVKKHESGMIINPMTVH
                     PSQRVQEALDVMLKHNITGLPVTEEDGRLLGIITYRDLRFEKNLDFRVDKLMTPKERL
                     ITVTEGITLAGAKELLHKYKIEKLPVVDSEFRLRGLITMKDIEKIEKHPNASKDEMGR
                     LRCGAAVGVGPERESTIEALLKAGCDVIVIDTAHAHSKRVIDAVASTKSNFRKINLVA
                     GNVGTADGAEALVKAGTDAIKVGVGPGSICTTRVIAGVGVPQVTAIGDVARVAQRYDI
                     PVIADGGIKYSGDITKALAAGARSVMIGNLFAGTDEAPGEVVLYQGRTYKVYRGMGSI
                     EAMKKGSRDRYAQDDMIESKLVPEGIEGRVPYKGSIAGMIYQLVGGLKAGMGYTGSRN
                     VEELRKKARFIKVTLAGLRESHVHDVIITKEAPNYRIE"
     gene            complement(13382..>13807)
                     /locus_tag="VI935_08255"
     CDS             complement(13382..>13807)
                     /locus_tag="VI935_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25626.1"
                     /translation="AFRYKDKCILLTGGQRGMKSTRFKISVSQDGLLPEETADVATLQ
                     FPILPKKLLKALGFERVDTKTYVGVLPVFVNIGDKEQEIVVELGVTVPEMRKKVIEVV
                     RTPSNLASPKRAVKPQKTTIRRNGRRPQRKRIKVRKSRR"
BASE COUNT         4288 a         2739 c         2649 g         4131 t
ORIGIN      
        1 atctctcttt tcttttgtaa ctaagacctc aaaatgaaga tattcatccg tattaagcgt
       61 caacccattt ggaagagaaa taaggctgaa aacagcacca tctcttgcct tgattatgtc
      121 ctgtatcctt ctttctgaat ctgtttcagg agaaggaaat ataaacttcg actctcgagg
      181 cgttggatct agaacataag gactttttac actcaagcca aaccaaaaga ggctattatc
      241 ttgatacctc cgtgttttgt acttacgaag aggatatttc ttttcgccat tactatctaa
      301 gagtgtaata tgccatttgt ctaactcttt ctccaaggaa aagtgatcta ttaatgtatc
      361 ggttggtaaa ttgaatttct cgatcttctg cttcccttca gaattccacc ttagacttct
      421 tttgacaata ccaggaaaaa acagaagtct ctcacctgga gtgtagcaaa aagccccaaa
      481 tgttcgtaaa ggcttattca gctcttgata aagaacatag gtaattctac ctataacccc
      541 attttcaaga actcttctta aaggtcttac aagaggaata aaaaaagaat cgctagttcc
      601 ttgtgtaagt tctttgggat tactaattgt gataactata gtctccatct tatgtttagg
      661 catgaaaata atgatttaac tcaaattgtt actaattatt gcaatagcat acttaataga
      721 aaaagcaaat atcaaaagaa aagcagagtt tagcaattaa agaatccctt gttagggagc
      781 gatgggggag gtccaaaacc atatgacctc tactccggat cgcaaaatga tacttgttat
      841 gctttgtttg gtttagtcta gaatgataat aatatcaaag gattagaatt tgttgtattc
      901 tatatcaacg caacaaaaca aaaaaaatac aaactaaaca tacactgaat cagaaaaacc
      961 aatagagtgc tgtaatgtgt aaaacaaaag aacaatgaaa gagattgata agattttacc
     1021 taatataaaa aaggctctta aaaccattta tggtgatcaa ctcttagagg tcattctatt
     1081 cggctcttgt gctagaggga cttttactaa ggattcagat atagatattg cagttgtgct
     1141 taaaggaagg gtaaatagga caaaagaaat agataggata tacgatacgt tatatgagtt
     1201 gattctagaa accggggagc tcatttccat atatccagtt tctgaggaag aaatacttaa
     1261 ctcggtctgg cctttatatc atcatattag aaacgaaggg ataaggattt gaaagaaata
     1321 gaaaccctaa aatgcaaagg gtagcaagag caattaagag tgtagagtta ctacttaaag
     1381 agggagattt cgatagttcg gtttcccgtt cttattatgc gattttttat gcaactgaag
     1441 cggttctctt aacaaaagaa ctaaaattct catcacacaa aagcgtgata acactatttg
     1501 gcgaacattt tctaaagacc ggtactttca agccagaaat gggaaggagg ctaagcaaga
     1561 cttttgagaa gcgcctaata ggtgattata gttttgaacc ccaagtaact atgaaagata
     1621 caaaagagac tttaaattgg gcaagggaat ttgttaaaga tataaggaat tatcttataa
     1681 aagaggaata tatgaaataa aagcagctat aagagtcttt ttcccaaaac aaacttgagg
     1741 ctataccaac aaataattta accattaggg aataactttt ataacttcca atgcggattc
     1801 gaacctgcta ctcttggctt cctagtctct cactcaatcg ccttaactaa gtctcttttc
     1861 aaatttgcaa tgtcttcagt aaattgtaaa gtttcattgt aagtgagaaa taaatagaag
     1921 cgcatttcaa atcatagcag ggataactat agattcagca aagattagta taaggccttt
     1981 aggtaacatt tcagctgtta cttttttctc ggtggcatat tcaaccccta attaggagta
     2041 gaaatggaga gtaattcttc agaagtcata aaagagagca aagacccttc atcagagtgt
     2101 gaaaaagcct ctatgctcga ggatcgttat tttcttgaag gtccgcgttc cagaatacag
     2161 gagttatttt ttaccattcg tgtcgtgata gagttcatta agggttttag agtgctccac
     2221 tttgtaggac catgcgttac cgtatttgga tcagcgcgac taaaggaaga ccatccttat
     2281 tatcaacttg cacgcaatgc aggaggacgt cttgttaaat taggatttac tgttatgact
     2341 ggaggtgggc cgggtgttat ggaggcagct aaccgtggtg ctaaagaagc aggaggtatg
     2401 tcggtgggat gcaatattac acttccagta gagcaaaagc ccaatccata cttggataag
     2461 tgggtaacct ttaatcattt ttttgtgaga aaggtattgc ttgtaaagta ctcctatgca
     2521 tttgttgtaa tgcccggagg agtcggaacc ttagacgagc tctttgaggt tataacactc
     2581 attcaaacaa agaagatact ggatttcccc gtggttctta taggaaaaga atacttccag
     2641 gatgcaatgg aattttttca acgtatggtg cttgagggaa caattggtat atccgactta
     2701 aatctcatac ttttgactga ttctttggac gaagcaattt cacatattga aaaacacgca
     2761 gttgaaaaat tcaaactgcg tcggcggaag gtaccaaaac catcaaggtt attgagggaa
     2821 taaattcatt ctagtggtgc cctaactaac ctccctcctg atcgctacca cagatcccgt
     2881 ggtgccctaa ctaacctccc tcgtctggcc caagctcgac caaagcccgc cgactaccat
     2941 agatccaatg gctctagaaa aaatctcact tttgttcact aacccggaac ccaagcattt
     3001 ttcatggcgg gcgatgcggg attcgaaccc gcgacctctg gcttcggagg ccagcactct
     3061 atccactgag ctaatcgccc acttctcaaa gtttgctatg ttttcagtaa attgtaaagg
     3121 ttcttttggt gaaaaccgat taaatctcgg ttatggattg aggcataaga cagcattttc
     3181 tcatgaaatg tacagttaga gagaacttgc cttgaaagag gttattcttt gtgagacaat
     3241 attaaaagta tgtaaagcaa agattgctga tacatgcaag gagataacag gatatggcaa
     3301 atatgcagaa gaaaagcgta aactcaccag acgaaacacg ctccttccca aaaggaaagc
     3361 tcgaacttgt tacggttgca ggcatcacgt ttggtcgtgc tacacttgag ccaggatgga
     3421 agtggtctga atgtgtcaag ccaatagcta aaacaaaaag ctgcgaggct cctcacaccc
     3481 agtatcatgt ttcaggaagg ttaagagtta gaatggatga cggcacagag caagaatttg
     3541 gtccaggcga tgtatcgcat cttccccctg gacatgatgg ctgggtagtt gggaatgaac
     3601 ctgtggtagt aattgacatc acaggtatga cccactttgc tgagcaggct aaaaaatagc
     3661 gatagcatat ttagcaacat ttaaccagtt acattttagc tagccttcta ttcgagaaac
     3721 aactacatag aggataggtc taactttagg tattggcagg gctctgattt ctttaaactg
     3781 ttttgctttt tgatgaggag aaaacatccg aactcctaac agtggcttta ggtaatgaga
     3841 tgtaaatagg gataataaaa agcatgataa caataatcaa aacagtattc ctagggttgg
     3901 ttttaattgt ggccagtaac ataactgctc atgctgaaga ttgggtattt ataaccctct
     3961 cacctgatgg agaatttgca ttgtatattg ataaagatag tctcgtattt tctgacaata
     4021 aaacctggta ctggagtaag cttgaatttg cagaacctca agagagtgta aaagaaggcg
     4081 aaatttattc aatattgcat tcctttatag agatagattg tgccaagaaa acctttacga
     4141 atccctatgc tatagaaaaa aagaaagacg gacaagttgt ctggaggggt catatacctg
     4201 ctaggcccga tataccaatc cctcctgata gtataataga gagagaattt gaatttgtct
     4261 gcattaaatt aatacctaga taaaaattag cttgtgatgg taaggcatag atatagcaga
     4321 agacttatat tgagctggaa aagcatttta aatgttctag tcattatgga tagttatacc
     4381 tatcaaatcc ctatgattta aaggagaact cgggacgatg gggcaaatca gaatatctgc
     4441 aagaaacatt ggggaaattg gttccaataa aattaaacct caataagttt cgattgccat
     4501 ttataagagt aagagagaga tataatatgc cgagagtgcc ccaagggatt gaaggttgca
     4561 agagttgtga gtcattagac attcttttaa aggttgctac tggagagaaa tagtatttaa
     4621 gaaggttgcc ggtttaagga aaccaaattt caattattct ccttgtaggt gttttaacgc
     4681 cgatacttta tatttcctgt cattcctata aaaacctatc ttgcagggct tcggaggcta
     4741 tcactctatc ccctgagcta atcgcccgat ttaaagattc ataattcttt tatcacaatc
     4801 tgcatatcgt cccagagcgg ttttttatat ccagggtttc ttaaaagggc tgcaggatga
     4861 tatgtaacca taacctttgc ccttccatat gaatgaaact tccccctgag atttcccagt
     4921 ttttccttta ttcttaacac agattgagcg gcaacgcttc ccaaacagac aattatgtct
     4981 gggtcaatgg ctctaagctg cttaaagaga tactgctcgc aggttccggc ttcatcaggt
     5041 tcaggtgacc tgttgccggg tggacggcat tttacaacat tgcatatata tacatcggag
     5101 cgcctaagac ccattgcgat aattatcttt gtaagcaact gccctgctct tccaacaaag
     5161 ggtctaccct gtttgtcttc ttcctcacct ggaccctcac caacaaaaac gagccttgcc
     5221 tttggattcc cttccccaaa gactatattt gtcctccctt gatgtaattt gcaccttgta
     5281 cagtccccga tttcttctct gatttcttca agggtcagtt tcttagatgg tttgtcaaaa
     5341 atacctagct gctcctcgat gtatattcta gtccttttct ctgtaagcac tgaatatttt
     5401 aattcttttc tgtacgtttc agaactctct tcattctttt tttctcttgc caaggctatt
     5461 gtatcaagcc ctagaatctt ttgacactca aagtatctct tgagttcttc cagtaattcc
     5521 tttaattcaa gctctaagct catcagatgt attttaaagt tctaatataa tccaatgtcc
     5581 atatcacttc aactcgatga ttctatttag ctcgctaaag gttttctaac gtcattgcta
     5641 taaaaatacg gaaatgtcat tgtgaggagt caaacgacta gcaaactaaa aacgagattc
     5701 ctcgcttcgc tcggaacgac agaaagagag aatacttcaa tgagtttaca ctgagcactt
     5761 cactccactc actacaggct tcgcaaatgt gttcgcaatg acaacttgaa aacggagggt
     5821 gatcattaga acaacttgca caactttttc ataaatgacg aaagcaaatg gaattgcttc
     5881 accccattac tcctaacaat aacatatgcg aagttttatt ccctcttact acgcgctcaa
     5941 ctagatatgt tctataatct tataactttg ctaatctaaa aattaatgta atcttaatcg
     6001 gcaagaatcc gaaatataag aatttaatgt tatcttatct tttattttaa aaaggatgac
     6061 cgttgtcatt actaaagatt tcagtttcta taaaaacttc gaattatcga agctaaggca
     6121 cacacaatta ttcgtatgtt ggggttagta gttaatccct gccattagaa tcataggtta
     6181 tttaatcaac cttaaaatat gaaggagcaa atataatgaa gatccttgtt actggtggtg
     6241 ctggatttat cggttcatgg gtagctgata cctttataaa taagagacat aaggttttaa
     6301 tagttgatga cctttctact ggaatcgaag ccaatattcc gaaagatgca gagtttacta
     6361 aaggtaatat tagagaattt gcattactgg agaaggtctt ttctaatttc aaacctgata
     6421 tagttaacca ccatgcggca cagataaatg ttaggcgatc tttagaggat cctgtatttg
     6481 atgctgaggt aaacattatt ggaacgctaa accttttaga gctttctgta aaacacaaca
     6541 taaaaagatt cgtctttgcg tcatcgggag gcgctattta cggcgaacct gaaaatttac
     6601 cagcagacga aaatacttgt cctattccta tctcacccta tggagtttca aaacacgcta
     6661 ttgaaaaata cctaggttat tataaaacgg ttcataggct tgattacgtt gcccttagat
     6721 actcaaatgt ttatggcccg agacaaaatc cacatggaga ggcaggcgtc ttagctatat
     6781 tttgcaatag gattatggca ggaaaacctt gcgagatctt tggtgatgga aatcaaacaa
     6841 gggattatgt attcgtgggt gatgtaacaa atgctaatat acttagtatt aattcaccct
     6901 ctggaattta taacattgga acacagatag aaacctcagt taatgatctt ataactacac
     6961 ttagaaaagt aactaaaaga gaattcaaag tagaatactc tccaccacga gctggagagg
     7021 ttcttaggat ttacctagat attcaatttg ccaagcgcgt tttaggctgg tctccaaatg
     7081 taaatttaga agatggcata catcaaacct ggaaatggtt taatgatcgt gactcgtgat
     7141 tagtgatccg ggcttcagga atctaatatc aaattacaaa ctacgagtca cgaaccccga
     7201 gctaagagaa aaaaatggat ttttcagaac tcactcgaat aagcagtgga tttagccact
     7261 cgaggattct tcaggttgca ctcaagcttg gtgtatttga tgtgattaag aggcaagggg
     7321 gttcttcggt agaagttgct aaagctctcg gtacaaaccc tagggcgaca gagctttttc
     7381 tcaatgccct tgtggctctc agattcttac gcaagaagga agggaaattt tacaatacta
     7441 atacttcact taaatacctt gctaaagatt cacctaagtt cttcggagga atgattcttt
     7501 ttgaagaagg actatggaac atgtgggcaa aactagagga atctattcgc actggcaaac
     7561 ctgcgcgagc tacagatatg tttcagaaaa gcgaagaaga gacggagaga ttcattatgg
     7621 ctatgcactc acttgtgagt gcaaggggtg atgctgagat tctaagcaat aaacttgatt
     7681 ttagttgggc tcaaactatg attgatattg gttcagggcc aggatcatac cctataaaat
     7741 ttttacaaag ataccctcac ctgaaaatta cggtcttaga cctccacgga acactaaaag
     7801 taacaaaaca ggtcttaaaa aaagagggaa tgaatcgaaa gatcaagctt ttagagtgtg
     7861 attacaactc agatgaaatc cctaaagggt ttgatgtagc atttctttca aacatcattc
     7921 acagtgaaga tgaagaatcg aatgagaaac tacttaaaaa aatatatgaa tcgctaaatc
     7981 atggtggaga aataataatt aaagaccata ttcttaatca caccctaaca agtcctgcgg
     8041 ttggggctat attttcaatt caaatgctcc ttacaactaa gggacgggat tattcattcc
     8101 gagaagtaag aggctggcta atggatgtag gattcaagaa ggtcaaatgg ataagattag
     8161 aacctccgct tacatcgtct ctagtaatag gatggaaata atccattact acagctcagt
     8221 ttacaaatcc tagtgtagca aagaatgaca accaactctt cattaattga taatcggact
     8281 ttgtctgggt aatattaatt ttacggaggt tgaacatgat gcgtagatat gaaatttatt
     8341 ttgacgtgag agtaatcgag agagacattc gcgaaggaac tatcacgaaa aaggactatg
     8401 aagagtattt aaaaaaactt agcgacatat ctgagaaggg ttgtacactt gtaattgatg
     8461 aagcaacaaa tcagccatca gaaataacaa acagtgaatc gggagaaaat ggatgaaatg
     8521 ggactataga gttattccta tagatgagct attccctgaa catgatgacc atgatatagc
     8581 tgtgtcaaag caggctgcaa caactagaag agcgaaaata ggaagcggct ttgagcagaa
     8641 tttacacaaa cttggcaacg agggttggga gtttgtaaac atagttggcg aatttgggat
     8701 ttttaaaaag ccgagttaaa taaagggata taatcataaa tttccctcaa atattaatag
     8761 ggcaaaatat aacgagtgga tataatttta ggatttttaa cctttggcag ttgactaact
     8821 aggatgtgtt aaatttatac atgagagttt aaacttcaga attaattctc atcacattct
     8881 gtgacaagca attgcattcc gaaatgaatt atctaaaagt aataatatga atctcacttg
     8941 tgtaagatca ggggttaata atatatttta tctttttgca ttgtttctta ttcttacttt
     9001 acctaattct gcattttcgg taacagtctc ggtagaaggc tttgaaccat tatctggtaa
     9061 aactattgct tcggctagaa ctatggcact atcaaatgcc ctcaagactg cagttgaaaa
     9121 cgctgcaaaa gggattaacc ctactttaga cgctaagtca ctagagaaat taataaacga
     9181 gaaccctatt acatacataa aggcttacag gattcttgat gaaaatcgga ttggagatga
     9241 ttataaaatc atacttgaag ctaatgtgga ttccgagatg cttaaagaca aaatcaaagg
     9301 tttgaaggtc aataatctta aatctcccgg aaagccctcg atatcaatag tagttttaaa
     9361 aatcccagag agtgacccga ttataaagag cttacccctc tcggaaataa agagtgagat
     9421 tacaagagta cttcaaggtg ggggttataa ggtccaggaa tccatgggag atattagaat
     9481 agaaacctat gtgagtgtaa agactagtga aagcaaagta ggtagaagca tgtgttatgc
     9541 ccttgggaac gtattaataa aagtaaaaga taaaaacgaa aaggtcttaa acgaggtaag
     9601 tgatagctta tatttgaacg gtaccgacat aactcaactc agccttgagg caataaaaca
     9661 ggctggagca aaggcagcag ataaggttaa attggagttt gataaaaaat ggaatattgg
     9721 tcaacgagtt aaaagtaaaa caatattaat aacctttgtt ggatttagta actacaaaca
     9781 atacgaacta ctagacacaa ttttagcaaa ctcgacccca ggcattgata atataacaaa
     9841 gaggattttt agaaatgatg gagtttcctt tttggtactc tcaagcataa gtgaagaaga
     9901 cctcgctaaa acagtagata gtcaaaacac acctggtttc tcacttaggc ttaatgaagt
     9961 ctcttctgat agaattgaat tcagtgttgt tactgagtag agacgccatt aatggcgtct
    10021 ctactcataa tgtgggttaa aaaatccgaa atccgcgatt agcattccgc atttcttata
    10081 tctctattcc cactcaattg tgcttggggg ttttgttgag atgtcgtaaa caacacggtt
    10141 aattcctttt acttcgttta taatccgtgc tgagattttt tcaaggattt caaaaggaat
    10201 gcgagcccaa ttagcagtca taccatcaag gcttgtcaca gccctaaggg caataacatt
    10261 ttcatatgtt ctttcatccc ccatgacacc tacggttttt acagggagta gtacacagaa
    10321 cgcctgccaa atatcattgt aaattcccgc cctttttatc tcttctatga aaatagcatc
    10381 agcgtacctc aacttctcaa gcttttcttc tgttatttct cccatgattc ttatcacaag
    10441 gccaggtcca gggaatggat ggcggtcgag gatttcagaa ggaagcccta gttcccgccc
    10501 cacagctctt acttcgtctt taaatagctc tctcaagggt tcgattagtt tcaggttcat
    10561 cctctcagga agtcccccca cattatgatg ggttttaatc tttgctgagg gaccctttac
    10621 actaacgctt tctataacat caggataaag cgttccctga gcaagaaact ccgctccttc
    10681 tatctttgag gcctcgtcct caaatacctt aataaattcc tccccaatta tctttctttt
    10741 catctctggg tcttctattc ctcggagttt atcaagaaat cttcttctag catttacatg
    10801 agttaaattg agtcccaagt ctttaaaagt ttcaactacc tgttttgctt cgttgagtct
    10861 cagaagccct gtatcaacaa atatgcaatg gagtttatcc catatagcac gctggacaat
    10921 taatgcagta accgccgagt ccactccccc tgagagcgca caaactacat gagaatttcc
    10981 taccttctgt ctaatctcct ttatggcggt ccccgcaaat gatttcgctg tccatgaacc
    11041 ttggcatttc gatattttaa taacaaaatt tgagagtatt tccttaccaa gctcagtgtg
    11101 aataacctct ggatgaaact gagtgccgaa gattctgccc cctgtatgct tcatcgccgc
    11161 tactggcgag ttctcagtca tcgctataga aacgaaaccc tttggaagct caagcacacg
    11221 gtcgccatga gacatccaga ccctggtcat tcgaggaata ttcgagaaaa tatccaaggt
    11281 atctgtgact tcaatttccg caggtccgaa ttcacgcctt gttgagcgct ctacctttcc
    11341 gcctagaaga tgagctatga gctgcatccc atagcatatt ccaaggatcg gaaccccaag
    11401 gttaaataag ccgggatcaa gtgacggcgc cccttcatcc catacacttg cggggccgcc
    11461 tgaaagaata atcgccctcg gttgagtttc ctttattcgt tctaacggtt cgttaaacgg
    11521 tttaatctca gaataaacgc caagctcacg tacccttcgt gcaataagct gggtgtattg
    11581 agaaccataa tcaagaacta aaacggtttc acgcatttta aatctttgaa acgtttagtt
    11641 gataaataat atcattttca ttttgaatga ttgcattgat atcattcata atattatccc
    11701 gccagcaagc ctgtcccgag tttaacgaag ggactagcgg tctaccctaa tattatgggt
    11761 agccaatgag tcttgctcat tggcgtaaac agacgacaac cttaatccaa aattgtgaca
    11821 tgaaattgat atcagtttag taatatactg aataataata aaatcaatta gacaggtaga
    11881 caaacgatat taaatctaac tagggactct attcgattct atagttcggt gcttctttag
    11941 tgattattac gtcatgtaca tggctttcac gaagcccagc tagggtgacc tttataaacc
    12001 tagccttctt tctaagctct tcgacatttc tggatcctgt atatcccatc ccagccttta
    12061 atccaccgac aagctggtaa atcatccccg caatcgagcc tttataggga actctaccct
    12121 ctatcccctc aggaacaagt ttcgattcta tcatatcatc ctgtgcatac ctatcacggc
    12181 ttcccttctt cattgcctca atcgaaccca ttccacgata gaccttgtat gttctccctt
    12241 gatatagaac tacctctcca ggggcttcat ccgtgcctgc gaagagattt cctatcatta
    12301 ccgagcgtgc ccctgccgcc agagccttag ttatgtcacc tgaatacttt atcccaccgt
    12361 ccgcaattac agggatatcg tatctttgtg ctactcgtgc cacatctccg attgcagtaa
    12421 cctgcgggac tccaacaccc gctataactc gagttgtgca tattgatcca gggccaactc
    12481 caacctttat cgcatcagtc cctgccttta ccaaagcctc tgctccatca gcagttccaa
    12541 cgttccccgc tactaagttt attttacgga aattagattt cgtggaagct actgcatcga
    12601 ttaccctctt tgaatgtgcg tgtgcagtgt caatcactat gacatcacac ccagctttaa
    12661 gtagggcctc aatagttgat tctctttcag gtccgacgcc aactgcggct ccacacctta
    12721 agcgtcccat ttcatcttta ctagcatttg ggtgcttctc tattttttct atgtccttca
    12781 ttgttattag cccgcgtagc ctgaactcag agtctactac tgggagtttt tcaatcttat
    12841 atttgtgaag aagctctttt gcgcctgcga gtgttattcc ctcagttaca gttatgagcc
    12901 tttcttttgg ggtcattaac ttatcaaccc tgaaatctag gttcttctcg aatctgaggt
    12961 ctctataggt aattatccca agaagccttc catcctcttc agtaactgga agtccagtta
    13021 tattgtgttt tagcatgaca tcgagtgcct cttgaaccct ttgtgaagga tgaacggtca
    13081 tagggtttat tatcattccg ctttcatgct tttttacctt ttctacttct gcaacttgtt
    13141 cagctaaagt tagattccta tgaatgaccc caataccacc ttcttgagcc atagctattg
    13201 cagtttttga ctcagtaact gtatccatgg ctgcactaat aagtggaata tgtagcttga
    13261 tttctgtggt caaataagta gagacatcta cttcagtagg tagaacaccg gtataatctg
    13321 gcaataatag aacatcatca aaggttagaa attcctccat tttattctca aacatagaag
    13381 cctaccttct tgactttcta accttaatcc tttttctttg gggccttctc ccgttacggc
    13441 gaattgttgt tttttgtggt tttaccgccc gctttgggga ggcaagattt gaaggggttc
    13501 ttacaacctc tattaccttc tttctcatct cgggaactgt aaccccgagc tcaaccacta
    13561 tctcttgctc cttatcacct atattcacaa aaacaggaag cactccaaca tatgtttttg
    13621 tatcaaccct ctcaaaacca agcgctttta gaagcttctt aggtaaaatc ggaaattgta
    13681 atgttgctac atctgccgtt tcttccggaa gcaaaccatc ttgactaaca cttattttaa
    13741 atcgcgtaga tttcatgccg cgttgtcccc ctgttaataa gatacactta tccttatatc
    13801 taaaagc
//
LOCUS       DATDRL010000242         7130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2932909, whole genome shotgun sequence.
ACCESSION   DATDRL010000242 DATDRL010000000
VERSION     DATDRL010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2932909"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            365..1351
                     /gene="thiL"
                     /locus_tag="VI935_08260"
     CDS             365..1351
                     /gene="thiL"
                     /locus_tag="VI935_08260"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HLE25627.1"
                     /translation="MIDELSALRLIKERFQKVSKNIAIGIGDDAAAVKINPESLLLAT
                     VDSQVEGVHFVKGVISAKELGRKSIAVSVSDIGAMGGIPKFFLASVGFSGEEDEGFLE
                     QLMDGFQSGAEEFKVDLIGGNLSSSQNLFLDITVLGEVEQHLMVKRSGATPKDIIYVS
                     GTIGDSALGLRVLQSKTNREKNWFLISRHILPQPRLKLGRELAKRRLVTSMIDISDGL
                     ILDLERITIEHGLGAEIYIERTPISSYYRDCISDFTKDLYDLALSGGEDYELLFTSPK
                     EKREEIKEISEKLNIKITEIGYVTKNPPVRVLDANGKEIKVKRRGFIHFGNY"
     gene            1467..2810
                     /locus_tag="VI935_08265"
     CDS             1467..2810
                     /locus_tag="VI935_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HLE25628.1"
                     /translation="MIDLPLNYSLFLIVILLMLSAFFSMSEGSLFSLGRHQREKLKKE
                     GKRSSTLIERLLKDPYKLIITILLADEVINVAYTSTVTSTLRKIIHLPSDQIITLICI
                     GVASPTLLLLGEIGPKTIGVKYPRLLSTLVSYPLNLVHILITPFRWIIMVLSIGLTWL
                     LGGKIEHEQRTGFNPDEVKALVGLGSEEGVVTDIERKLVGSLFKLEDVPAYKIMTPSI
                     DCFFLPVNIAPLDAIIEIKKRGFSRVPVYKGDRDNIVGILYVKDLLSATTIDGLTLES
                     LVRTPYFIPRTKRAFDLLREFQQKRIHIAIVVDEYGRVDGIVTMEDLLEELFGEIEDE
                     RRVMKEHTIKKDGEALLIPGSLKIDEFNDTILFSVLRFGGLENLASELEASILPPEDD
                     HETMGGFVFDLFGRFPHEGEHITYGSIVFTVNKVSGKRIAEIKVERLKKEGANVA"
     gene            2800..4023
                     /locus_tag="VI935_08270"
     CDS             2800..4023
                     /locus_tag="VI935_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HLE25629.1"
                     /translation="MSLELVIIIITICLLLQAFFAGSEIALVSCDKIKMKALAEGGST
                     RAKLVLKSFVEIERYISTTLAGINLSLITSTVILTFYIQDTYGKGKEIYTVLILSPLI
                     VIFGQVVPKAVFQKRASTMVLWSIYPLWIASKVFSPILFQINIFIKGLLRLIGSTENP
                     FITREELLHAIEGDETIRQGGYREKIIKRIFRFSETSVDEIMIPLIQVCALNEDTQAK
                     DAVKIIKETGHSRIPIYHERVDNITGILHAFDILGAKPDDPIKNFTLTPFYVPETKSV
                     DELLDEMKKGSAGMAVVVDEYGGAVGVITLEDIIEEIVGEIEDEYDKGIRLWRKISES
                     EYQINPKIEIEKVNEELGLGIPEGEYDTLSGFLLSEVGSIPKVGEEIPFKNHTFIVTK
                     ASLRSIEEVKLIAGR"
     gene            4570..5157
                     /gene="hisB"
                     /locus_tag="VI935_08275"
     CDS             4570..5157
                     /gene="hisB"
                     /locus_tag="VI935_08275"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HLE25630.1"
                     /translation="MERRANIKRKTSEVEVSVDLNLDGKGKYDIETGIPFFNHMLSQF
                     AKHGYFDLTLKAKGDIEIDYHHTVEDVGLALGEAFLKALGDKRGISRFGDAFVPFDDT
                     LVFASVDLSGRPYFVYKVRTPKSKVGDFDIELGGEFFKSLSNAIKCNLHIEQKYGNNL
                     HHIIEATFKAVARALDHASKIDPRSNEIPSTKGKI"
     gene            5350..5982
                     /gene="hisH"
                     /locus_tag="VI935_08280"
     CDS             5350..5982
                     /gene="hisH"
                     /locus_tag="VI935_08280"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HLE25631.1"
                     /translation="MISIVDYGMGNLRSVKKAFESLGFLPTVTSNPEEILNSGGLVLP
                     GVGAFGDCMKNLEEYNLIEPIRLFIKDGKPFLGICLGLQLLFEESEESPGVNGLGILK
                     GKVVRFPRFDEGRLKVPHMGWNQVDARRPLPILKGIPVGSWFYFVHSYFPEPEDSTVI
                     AGITHYGIEFTSAIEKDNIFACQFHPEKSSTLGLRILENFAVMCGEKQRQ"
     gene            complement(6194..>7130)
                     /locus_tag="VI935_08285"
     CDS             complement(6194..>7130)
                     /locus_tag="VI935_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLE25632.1"
                     /translation="REALVTFSSFTNSESPNNSARATYWKARILEKMGRKEEAINIYE
                     SLARSDNPTYYSYTALKKLGSIPKLNISLNSNTKTDMVKKVNPRKSKAELLIELGTFD
                     DAILEILELEKEATNTWDLMDVSRLYNRASKFNHSIKIAQDIRLSEAYRLSYPKGFNE
                     IVRLHSGKYQADEFIVYSIIREESRFQDDAVSRSGAIGLMQLIPDTGRSTAQKVGISG
                     YNTDMLYIPVVNVELGTAYFQEVLEQYNGVVYLAIASYNAGPHNVARWVEKLNNLEVD
                     EFIEEIPYQETRNYVKRVLRSYGVYKAIYDSGNAQ"
BASE COUNT         2213 a         1351 c         1494 g         2072 t
ORIGIN      
        1 ttattaatcg taagccatca cgatcccatt tgccattttt ctataaaaag aaatctctca
       61 ataaatatta gatgattttt acccttctac tttctgtagt ggttaaccac attccagaac
      121 aggtttggtt agccttttta aatacctagg tttgacatac aaagtcaaat atcctaaaat
      181 ggtatcgtgg gtattgatta ataattgggt tgataaccta agtccttata tgcaaatagt
      241 aattcttaat tatttaaggc atatatctaa ttcacttaaa ctttttacca atcagataat
      301 accctccctt acgctttatg cgattgcctg cgagggggag agataccttt aggttttaac
      361 taaaatgata gatgagctta gcgctcttag gttgattaaa gagcgatttc aaaaggtatc
      421 aaaaaatatc gctatcggga ttggagatga cgccgcggcg gttaaaatta accctgaaag
      481 ccttttgcta gcaacggtag attcacaggt agagggtgtt cattttgtaa aaggggttat
      541 ttctgccaaa gagttaggca gaaaatctat tgctgtatct gtgagtgaca taggggcaat
      601 gggtgggata ccaaaatttt tcctggcttc tgtgggattc tctggagagg aggatgaagg
      661 ttttctcgaa cagttaatgg acggatttca atccggtgca gaagaattta aagttgatct
      721 gattggcggg aacctaagtt cgtcacaaaa cctcttcctc gacataacag ttctcgggga
      781 ggttgagcaa catcttatgg ttaaaagaag tggtgctacc cctaaggata ttatctacgt
      841 gagtggtaca attggcgact cagcactcgg attaagagta cttcaaagca aaacgaatag
      901 agaaaagaat tggtttctaa tttcaagaca cattttacct cagcccagac ttaaactcgg
      961 aagagagctt gctaaaagaa ggcttgtaac atcaatgata gacataagcg atgggcttat
     1021 tcttgacctt gaacgtataa caatcgagca tggattaggt gcagagattt acattgaacg
     1081 gactccaatc tcttcttatt acagagactg tatatctgat ttcacaaaag acctttatga
     1141 ccttgcactt tcagggggag aagactatga gcttcttttt acttcaccta aagaaaaaag
     1201 agaagaaata aaagagattt cagagaaact caatatcaaa atcacagaga ttggatacgt
     1261 aaccaaaaac cccccagtca gagtattaga tgcaaatggg aaagaaataa aagtgaagcg
     1321 aagagggttt atccactttg gcaattatta ggtacgaatt gacacaaatt tgccgatttt
     1381 ggatttcgga attggaattt cggatttttt taatccgcat cccgcgcaga gacgccatta
     1441 atagcgtctc tgcaagatag taacaaatga tagatttacc tttaaattac agcctttttc
     1501 taattgttat tcttcttatg ctctcagcct ttttctccat gtctgagggt tcgctctttt
     1561 cccttggcag gcatcagcgt gagaaactaa aaaaggaggg gaaaagaagc tcaactctga
     1621 tcgaaaggtt gcttaaggac ccttacaagc tgattattac gattctcctt gcagatgagg
     1681 taattaacgt tgcttatacg agcactgtta cgtcaacctt gagaaaaata attcatttac
     1741 cctccgatca gataatcaca ttaatctgca ttggggttgc atctcccact cttcttctcc
     1801 ttggtgaaat aggaccaaaa acaataggtg taaagtatcc gagactttta tcaacccttg
     1861 tttcatatcc gctcaacttg gtacacatat taattactcc atttaggtgg ataataatgg
     1921 ttctctcaat agggcttaca tggcttcttg ggggaaaaat agaacatgaa caaagaaccg
     1981 gttttaaccc agatgaggta aaagcccttg tagggcttgg aagtgaagaa ggggtggtta
     2041 cagatataga gaggaaactc gtaggaagtc tctttaagct tgaagatgtt ccagcttata
     2101 agattatgac ccctagcatt gactgctttt ttctccctgt gaacatcgct cctctcgatg
     2161 caatcatcga gatcaagaaa agagggtttt caagggtccc cgtttacaag ggtgatagag
     2221 ataacatagt tggaatcctc tatgtaaaag acctcctctc cgcaaccact atagatggac
     2281 ttacgcttga gagtcttgta agaacgcctt attttatccc acgaacgaaa agggcttttg
     2341 atttactgcg tgagtttcag caaaagagaa tccacatagc aattgtcgta gatgagtacg
     2401 gaagggtaga cggtattgtt actatggagg acttactaga agagcttttt ggcgagatcg
     2461 aggatgaaag aagggttatg aaagagcaca ctattaaaaa ggatggagaa gctcttctta
     2521 tacctggaag ccttaagata gatgaattta acgacaccat tctttttagc gtgcttagat
     2581 ttggaggtct tgagaacctt gcatccgaac ttgaagcatc aattcttcct cctgaagatg
     2641 accacgaaac aatgggcggt tttgtgtttg acctttttgg aagattcccc cacgagggag
     2701 agcatattac ttacgggagt atagttttta cagtcaataa ggtttctgga aaaagaatcg
     2761 ctgagataaa agtagaaagg ctaaaaaaag agggagctaa tgtcgcttga gttagttatt
     2821 attattataa caatttgtct tcttcttcag gccttttttg caggctctga aatcgcactt
     2881 gtttcttgtg ataaaatcaa gatgaaagcc cttgccgaag ggggttcaac aagggcaaag
     2941 cttgtgctta aatcctttgt agaaattgag cgctatataa gcactactct tgccggcata
     3001 aatctatcac taattacaag cacggtcatt ctaacctttt acatacaaga cacgtatgga
     3061 aaaggaaagg aaatctatac tgttttaatt ctctctccac taatagtaat ctttggtcaa
     3121 gtcgttccta aagccgtttt tcaaaaaaga gccagcacta tggttctctg gtccatctat
     3181 ccgctttgga ttgcatcaaa ggtattctcc cctattctct ttcaaataaa catttttata
     3241 aaagggcttt tgagactgat tggaagtaca gagaacccat tcataacaag agaggaacta
     3301 ctccacgcta ttgaaggcga tgagacaata cgccagggcg gatacaggga aaagattata
     3361 aaaagaatat tcagattttc tgagacaagt gtggacgaga taatgattcc attaattcag
     3421 gtctgcgccc tgaatgagga tactcaagcg aaagacgctg ttaagataat aaaagaaact
     3481 ggacattcta gaatcccgat atatcacgaa agagtggata atatcactgg aatactccat
     3541 gcatttgata tactaggtgc aaaaccagat gatcctataa agaatttcac gctcacgcca
     3601 ttttatgttc ctgaaacaaa gtccgttgac gagcttcttg atgagatgaa aaagggatct
     3661 gcagggatgg cagtagttgt ggatgaatac ggtggagcag taggagttat caccttagaa
     3721 gatattattg aagagatagt tggtgaaatt gaagacgaat atgacaaggg gattaggcta
     3781 tggaggaaaa tctcggagag tgaataccaa ataaacccaa agattgagat agagaaggta
     3841 aatgaagaat taggactcgg aatcccagaa ggagaatatg atacgcttag cggctttctt
     3901 ctttcagaag tgggttctat ccctaaagtc ggagaggaaa tcccttttaa gaatcacacc
     3961 ttcatcgtga ccaaggcttc tctaaggtct atagaggaag ttaagttaat agcgggtagg
     4021 tagaatgtag atttcatcct gtccttctta gattcccact aaaacttgta cccgcatgct
     4081 ttaagcgagt aacatgcggg aatcagagac cttctgtcat tcccgtcctc cggttaaacc
     4141 ggaggatcaa tcgggcatcc atactatatt cttttatccg aggttagaaa acctcggcta
     4201 tcgagttgtg attaaatgaa aataacattc gatagcagcg actttctagt cgctgatttt
     4261 aataagggga atttatcgcg tctagcctat cctgagcggg ctgtcagtag attttgagca
     4321 agacttataa tccacccata atacatgcgg gatgtaaata aaaaatggtt ctaccaacgg
     4381 tttatcaccg aggtagacca ccgattcttt tgagacctca ggacaggctt actggtggtc
     4441 agttagtagg acataagcaa ggccagttgc ctacttagcg tatttttgat caacttacat
     4501 aaatccgaca actgtttctt agtacttatt tttggctaag tggtcaaatt tcagatatta
     4561 ttctgctaaa tggaaagacg cgctaatatc aagagaaaaa cctctgaggt agaggtttcg
     4621 gttgatctta acctagacgg gaaggggaag tacgatattg aaacaggtat tccttttttc
     4681 aatcatatgc tctcccagtt tgcaaaacac ggatactttg accttacgct caaagcaaag
     4741 ggcgacattg agatagacta tcaccataca gttgaggatg tcgggcttgc tttaggtgag
     4801 gcttttctca aagccctagg agataaaagg ggaatatcaa ggtttggaga cgcgtttgta
     4861 ccatttgatg atacgcttgt atttgcatcg gttgatctga gcgggagacc ttattttgta
     4921 tataaagtac gcacacctaa aagcaaggtt ggagactttg acatagagct cggaggagag
     4981 tttttcaaat ctctttctaa tgcgataaaa tgcaatctcc atatcgaaca aaaatatggc
     5041 aacaatcttc accatataat cgaagcaaca tttaaggctg ttgctcgtgc acttgaccat
     5101 gcctcaaaga tcgatccccg ttcaaatgaa ataccatcaa caaagggaaa aatctgattt
     5161 ataagccaag gactgaatca gacgaaagaa aattaagata gttgagatat ttgacacaag
     5221 atgcacgata cacgatgcag gatgcatgta aatacttcgt gaatcttgaa tcatgcatca
     5281 tgaatcatgc atcggtttat tagggcaagt tcgtgtttat tagtggctaa ttagatacag
     5341 agaacaccta tgatttcgat agttgattat ggaatgggaa atcttagaag tgtaaagaag
     5401 gcgtttgagt cccttggatt tctacctact gtgacaagta atccagaaga gatcttgaac
     5461 tcaggtgggc ttgttctccc cggtgttggc gcattcgggg attgtatgaa aaacctagaa
     5521 gaatacaacc taatagaacc gataaggtta tttataaaag atgggaaacc atttctagga
     5581 atctgccttg gtctccaact cctatttgaa gaaagcgaag aatccccagg tgtgaatgga
     5641 ctcggaattc taaaaggcaa ggtagtcagg tttccaagat tcgatgaagg aaggctaaag
     5701 gttccacata tggggtggaa tcaagtagat gcgagacgcc cgctacctat tctgaaagga
     5761 atacctgtcg ggagctggtt ttattttgtg cactcatact tcccagagcc tgaagatagt
     5821 accgttattg caggaataac gcattacggg atagaattca catccgcgat agagaaagac
     5881 aatatttttg cctgccagtt ccatcccgag aaaagtagta cactaggcct caggattctt
     5941 gaaaactttg ccgttatgtg cggggagaaa caaaggcaat gaataggcct gcggcaagct
     6001 gcagggtatc ggggtatcat acataaatct caccagttat ttagaagagc aaagcaacga
     6061 atcttacacc agttgtcatt ccgagcgctg cgaggaatct caagaaattg cttcacttaa
     6121 ttcgcattga cacatgggat aatgaaactc tgatgaaagc cacagggaat tatgaagcaa
     6181 aactgtttat tcatcattga gcatttccac tatcgtaaat tgccttgtag actccatagc
     6241 ttcttaaaac ccttttcaca tagttccttg tttcctgata tggaatctcc tcaataaact
     6301 catcaacctc caggttattt agtttttcta cccaccttgc cacattatgt gggccagcat
     6361 tgtagctagc aatcgcaaga tagacaactc cgttatactg ctctaacacc tcttggaaat
     6421 atgctgttcc gagctctacg tttacgactg ggatataaag catgtcagta ttatatccac
     6481 taatgccaac cttttgtgcc gtgcttctcc ctgtatctgg tataagctgc attagcccaa
     6541 tagcaccaga acgagaaaca gcatcatctt gaaacctgct ttcctctcta attatcgagt
     6601 atacaatgaa ctcatccgcc tgatattttc cagagtgtaa tcttacaatc tcattaaatc
     6661 ccttcggata ggagagccga taagcttcag aaagtctaat atcctgtgca atctttatag
     6721 aatggttaaa tttactagct ctattgtata gcctacttac atccatcaaa tcccatgtat
     6781 tggtagcctc tttctccaat tccagtattt ctaaaattgc atcatcaaaa gtacctagct
     6841 ctatcaacaa ctcagcttta ctcttccttg gatttacctt tttaaccatg tcagttttcg
     6901 tgttggaatt tagagaaata ttaagttttg gtatggaacc caacttcttt aatgctgtat
     6961 aggaataata ggttggatta tcagaacggg ctagactttc atagatattt atagcctcct
     7021 cttttctacc cattttttct aggattctcg ctttccaata ggtagccctt gctgaattat
     7081 ttggagattc agaattggta aacgatgaaa acgtaactag agcctctctt
//
LOCUS       DATDRL010000243         9223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_61623, whole genome shotgun sequence.
ACCESSION   DATDRL010000243 DATDRL010000000
VERSION     DATDRL010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9223
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61623"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="VI935_08290"
     CDS             <1..1409
                     /locus_tag="VI935_08290"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HLE25633.1"
                     /translation="IYGWRGADITNILNFESDFPDAKVVKLEQNYRSTKNILRAANAV
                     IVRNRYRRDKRLWTENTEGSAVVYYEARDERDEARYVASQIEYERTTDERSYKDFAVF
                     YRTNNQSRVIEEEFVHQGIPYNIVGGVGFYERAEIKDIMAYLRVIANPLDEISLRRII
                     NVPSRGIGKATIETLDKIAQERDISLLDATELAIKNELLSKKALASLEKFYNLINELI
                     KLSQRLSIGRLLDKILEKTNYLEMLEHDEERRDNLGEVLTLAAEFEKEEANNSIHDFL
                     DWITLSSDVDRFNEKADKVTLMTLHCAKGLEFPIVFIVGMEESLFPHIKSLGNGRLIE
                     EERRLCYVGITRSKEKIYLTSTSKRRLFGEEHRSIPSRFVTEIPRKLLQWESYQTNFG
                     EEAFKNHNNDQKRVDGNGFPKNGDGYIIGEKVIHPSFGQGVVKRVEGLGDEAKVVISF
                     HAHGEKKIIASFLGLKKI"
     gene            complement(1651..2262)
                     /locus_tag="VI935_08295"
     CDS             complement(1651..2262)
                     /locus_tag="VI935_08295"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HLE25634.1"
                     /translation="MPHELPKLPYSYDALEPHIDARTMEIHHTKHHQAYVNNLNAALE
                     KYPNLQNKSVEELIKDLNAIPEDIRTAVRNNGGGHLNHSMFWPMMSSHGGGEPKGDLV
                     AAIKSAFGDLASFKDQFSKAATGRFGSGWAWLCIDKNGKLVINSTPNQDNPISEGLKP
                     ILGLDVWEHAYYLKYQNRRADYIGAWWNVVNWEQVAKNYAAAK"
     gene            2552..3301
                     /locus_tag="VI935_08300"
     CDS             2552..3301
                     /locus_tag="VI935_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLE25635.1"
                     /translation="MREEFIIEIKDVHKSFGLKEVHTGVTLPVRRGDTITVLGGSGTG
                     KSVLLKEITGLVKPDKGEVIVEGEDIVPMDEGELIHVRRKMGMLFQGAALFDSLTVAE
                     NIAYPLREHSKHNEGEIKEIVAKNLELVGLPGIEDKIPSDLSGGMKKRVGLARAMASE
                     PNILLYDEPTTGLDPPNISRINHLIRDMQQKFGITGVVITHDVASAFEISDRIAFLYK
                     GKIIFEGTVEEAKNTVIDVLRDFINGKLEDD"
     gene            complement(3332..3805)
                     /locus_tag="VI935_08305"
     CDS             complement(3332..3805)
                     /locus_tag="VI935_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25636.1"
                     /translation="MKKYLILIIIAVVVLTYIFLPSGDDTREIEVILDGVIQAGREKN
                     LDGVMEHFSINYRDDYGATYPVVKNIVKTFFDKFDGIDGKYSDLTVSIDETEEGGKQA
                     VANFDISVSGIRSSIPTPILGSGDVPQNLIVTLRKSKLTGWKIVKVEGVELEENF"
     gene            complement(3815..6193)
                     /locus_tag="VI935_08310"
     CDS             complement(3815..6193)
                     /locus_tag="VI935_08310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HLE25637.1"
                     /translation="MNSFLSKYKILPPLSGFILGILLSEYFGNEWILITIISPVFGAI
                     YLIKPKLGFLIFIPIGLLFSAKPYLQENNVLNFVGKELDIEGVLYKSPESREKGSRLF
                     IDVDRILIGGKEKPSSGKVIITTEETITGLAYGDRIRVIEAKLRPIRSFQNPGSFDIR
                     RYYERQGVYARGFVPGKEWIISFGKSKGSNSFVHAVDKLRVKFANFVRKKSPFPESEV
                     LNAITIGDQGGIAPELRNEFSEAGIAHLLSISGLHVASVAFVFYLIIKWILKRSEYLL
                     LRFQVPRLTAALTILPVFIYMFVAGFATPVVRASIMAVAYLVSIVIGREENKLNTLAV
                     SAFIILLWHPWALFELSFQLSFASVLGILLMHRFYPFKLNTFKDKLFTSVKTTVAASF
                     ATLPFIINTFGTISIVSIPANLVAVPFVEFLIVPLGLITFLTFLVSEVVARVFLYGDV
                     FLIKLLILGTKELVKFPLSSLTVPSPGYKTWLVLIPSIIPLLFWKKYYKLRLLLPVFI
                     LVFLVTWAFTSLRNPNLGFLEVNFLDAGSRNITFIRLPIEKTILIDGGFSKVNMGGFI
                     EQSVVTPYLLKSGVTNINYLILTSLDKDHLEGVKNILQKFKVESIWTNGRKLDGELWG
                     IIKDKDIVWRNILDEETETLDIEGVEIELLKPEGEFNIRDSSLPYPILVRLTFKEVGL
                     IIGEGITDVNAITRLFEFNRDKIQNTVIYFPKVFMKQKESMDFIRMLSPKVIVTNSTL
                     ENFSKPLDERKLNRYYNPSVFQIDTQGLVTVLTDGKQLQVKTFLGKRVDIIN"
     gene            complement(6193..6672)
                     /gene="ispF"
                     /locus_tag="VI935_08315"
     CDS             complement(6193..6672)
                     /gene="ispF"
                     /locus_tag="VI935_08315"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HLE25638.1"
                     /translation="MHRVGIGFDAHKFSQGQKLIIGGVEIPFELGLEGHSDADVLTHA
                     ICDALLGAMGERDIGSHFPDTNSQYKGISSLVLLREVLTLSYSKGFMVENIDTVVVCE
                     KPRISGHVSHIKKKLASIIGVPEKSLGIKATTTDGMGFTGRGEGIAAYAVVLIKTVD"
     gene            complement(6795..7553)
                     /gene="ispD"
                     /locus_tag="VI935_08320"
     CDS             complement(6795..7553)
                     /gene="ispD"
                     /locus_tag="VI935_08320"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HLE25639.1"
                     /translation="MFNLKVAAIIAAAGLGKRFGEGLRKQFQPLSGKPLIVYSIERFE
                     DSKLINEIILVVPKDSINYCRREIKDKFQFKKITKIIPGGKQRQQSVKMGFISVSSET
                     DVVVVHDGVRPFVTARMIDEVIKESLNTGGAIAAIPVKDTVKKSSNKNHVVRTLSREA
                     LWLAQTPQAFQYNVLKSAYEKTENDGFLGTDESSLVERIGISVTLVAGSQTNIKITTK
                     EDLLLGELVLKEGTRLLDNDRYDHGEDIAQSERY"
     gene            complement(7546..8550)
                     /locus_tag="VI935_08325"
     CDS             complement(7546..8550)
                     /locus_tag="VI935_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HLE25640.1"
                     /translation="MKPKILFLILAAALGFWIAYPFYSFIIAIALGCILGAIAFVVIW
                     GLESRMNEFTPRAILGGGIGICVASLSFFAIKIVFDSLRIPSNITPYVLPIIFLSFFY
                     IGITIGAKKGFELDQTARKTSAKNNHRTPKILDTSVIIDGRIADVADTGFIEGIMIVP
                     KFIIKELQYIADSPDPIKRVRGRRGLDVLKRMQEIPNIVIKITSHDFPRVREADLKLV
                     ELAKSLRGIIITNDFNLNKVAGLHGVRVLNLNQLASALKPIVLPGETMNIRVVKEGKE
                     ENQGVGYLEDGTMVVVDDARKYVGREIEVSVTSVLQTPTGRMIFSRVKEESLISDLHV
                     "
     gene            complement(8787..>9223)
                     /locus_tag="VI935_08330"
     CDS             complement(8787..>9223)
                     /locus_tag="VI935_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25641.1"
                     /translation="KIANYQIIEIPNFNKNDKEWVPYDSASLIPDMVAEKLRTSNHFN
                     EITRSESNIASRESVLLVKGTVTGYKPGCKFCEFLAGRINDKGKSSVSVRVKLIDKTT
                     GDVIADAEMEGRAKNPGTGSSRYTRVVDEIVRLISNLGKESS"
BASE COUNT         2796 a         1775 c         1748 g         2904 t
ORIGIN      
        1 ccatctatgg atggcgcggg gctgatataa caaacattct taactttgaa agtgattttc
       61 ccgatgcaaa ggttgtaaag cttgaacaaa actatagatc aactaagaac atactgaggg
      121 ctgcaaatgc agtgatagta agaaacagat atcggcgtga taaaaggcta tggactgaaa
      181 atacagaggg ttccgcggtt gtttattatg aggctcgcga tgagagggat gaagctagat
      241 acgtcgcctc acagattgaa tatgaaagaa ccactgatga acgctcttac aaagattttg
      301 ccgtatttta tagaacaaac aaccaatcac gtgtgataga agaagaattt gtgcatcaag
      361 gaattcctta caacatagta ggaggggttg gtttttacga gcgtgctgaa atcaaagata
      421 taatggcgta tctgagggtt atagcaaatc cattagatga aattagcctg agacgcatta
      481 tcaacgtccc atctagagga atagggaagg caactataga gacacttgat aagatagctc
      541 aagaaaggga tatttcatta ttagatgcta ccgagcttgc tataaagaat gagctacttt
      601 ctaagaaggc gctagccagt ctagagaaat tttataacct aattaatgag cttattaagc
      661 tttcgcaaag gctctcgatt ggtaggcttc tcgataagat ccttgagaag acaaattacc
      721 tcgagatgct tgaacatgat gaggaaagac gggataacct tggggaggtt ttaacacttg
      781 cggcagaatt cgagaaagaa gaagcaaata acagtattca cgattttctt gactggatta
      841 cgctttcaag cgatgtggac agatttaatg aaaaggccga taaggttact cttatgacac
      901 tacattgtgc taaggggctt gagttcccaa tagtttttat tgttgggatg gaggagagtc
      961 tatttcctca cattaaatcc cttggaaatg gaagactaat agaagaagaa agaaggctgt
     1021 gctatgtggg catcactagg tcaaaggaga agatctatct cacaagcaca tctaaaagaa
     1081 gacttttcgg ggaagagcac agatcgattc cttcacggtt tgttaccgag atcccaagaa
     1141 agcttcttca atgggaaagc tatcaaacta atttcggaga agaggcattt aagaaccata
     1201 acaacgatca aaaacgagtt gacggcaacg gattccctaa aaatggcgat ggatatataa
     1261 ttggggaaaa ggtaatacat ccttcattcg gtcagggtgt ggtaaaaagg gtggaggggt
     1321 taggtgacga agcgaaagtg gttatctctt tccatgctca tggagagaaa aagatcatag
     1381 caagcttcct agggcttaaa aagatttaac cctctgaatc tcaagtggca tattgatatt
     1441 ttgtgggtcc aaccaatatt ttggttagaa atatttgtta taaatcagac tcataataat
     1501 tataactacc ctttattatt tactactctt ctgatctagt aaaggtggat tatttccctt
     1561 tcaaaatcgc ggaagtatca gttgatttac ttaagcaatt aattcctctt ctggtttgcc
     1621 cccaatatta gtgacaattg ctccaggatc tcatttcgca gcagcgtaat ttttggctac
     1681 ctgttcccag ttaactacat tccaccatgc cccaatatag tctgcacggc gattttgata
     1741 tttaagatag taggcgtgct cccaaacatc caaaccaaga ataggcttaa gaccttcaga
     1801 aattgggtta tcttgattag gggttgaatt tatgactagt tttccgttct tatctatgca
     1861 aagccaggcc caaccgcttc caaagcggcc ggtggcagct ttggaaaact gatctttaaa
     1921 actggcgaga tccccaaacg cggatttaat agcagctacc agatcaccct taggttcccc
     1981 tccgccatgg gaactcatca tgggccaaaa catactatgg ttaagatgac ctccgccatt
     2041 attacgtacg gcggtgcgaa tgtcttcggg tatggcgttt aaatctttaa tcaattcctc
     2101 aacactcttg ttttgtagat tgggatactt ttctagagcc gcatttaggt tatttacata
     2161 agcctggtgg tgtttcgtat gatgtatctc cattgtacgt gcgtcgatat gcggttctaa
     2221 tgcatcatag ctataaggta atttgggtaa ctcgtgtggc atattttatc cctccattca
     2281 gagttttttt gagtgattgt taggaataaa gaccaaactt agcattaaca tattttttat
     2341 tgaaaaattt tttgataagt agatatttat tattaacagt gctagtgtaa ctttttaatt
     2401 aagatgtgtc aaaggaatgt taaacagtag tttgttgttt ttgacaagct agcatataat
     2461 tattaaattt attgtagaat ttaaagtgat ccgaaccctc tgatatatac atcgtccatg
     2521 tttctattac tgattaagta agtaattaag gatgagagaa gaattcatca tagagataaa
     2581 agatgttcat aagtcatttg gcttaaagga agtacacacg ggtgttacgc tcccagtcag
     2641 aagaggagat actataactg tgctgggtgg cagcggtacc gggaaaagcg ttcttcttaa
     2701 agagataaca ggtcttgtaa aaccagataa aggtgaggtt atcgtagaag gtgaggacat
     2761 tgttccaatg gatgaaggtg agcttattca tgtaagaaga aagatgggga tgcttttcca
     2821 gggcgccgct ctttttgatt cactgaccgt agcggaaaat attgcttatc ctctaaggga
     2881 gcattcaaaa cacaatgaag gtgagataaa ggaaatcgtg gcaaaaaacc ttgaacttgt
     2941 agggcttccg ggaatagagg ataaaattcc ttctgacctt agcggtggta tgaaaaagcg
     3001 tgttgggtta gcaagggcga tggcttcgga accaaatatt cttctctatg atgaaccaac
     3061 aacgggactt gatccaccaa atattagtag aattaatcat ttaataagag acatgcagca
     3121 aaaatttggt ataactggcg ttgttatcac gcacgatgtt gcaagcgcat ttgagatttc
     3181 agatagaatt gcttttctct acaagggcaa aatcatattt gaaggaacag tggaagaagc
     3241 taaaaacacc gttatagatg tactaaggga ttttataaat gggaaactcg aagacgatta
     3301 ggctttcttc aatttaaggt ctcctaaatc attaaaaatt ctcttctaac tccacgcctt
     3361 caacctttac tattttccag cctgttaatt tagattttct taaggttaca ataagatttt
     3421 gaggaacgtc tccacttcct aagatggggg tagggatgct agaccttata cctgagacag
     3481 atatgtcaaa atttgcaact gcctgtttcc ctccttcctc agtttcatct attgatactg
     3541 ttaaatcgga atatttaccg tcaatgccat caaacttatc aaaaaaggtt tttacaatgt
     3601 tttttaccac tggatacgtt gcaccgtagt cgtctctgta gtttattgag aaatgctcca
     3661 ttaccccatc caagttcttt tcccttccag cctgtataac tccatctaaa atcacttcta
     3721 tttctcgagt gtcatcaccc gatggaagaa agatatatgt taaaactact actgctatta
     3781 ttattagaat tagatacttt ttcatgccta aagcttaatt tataatatca acccttttac
     3841 ctaagaatgt tttaacctgt aactgctttc catctgtaag aaccgttacc aaaccttgcg
     3901 tatcaatttg aaaaacactt gggttataat atctattcaa ttttctttca tcgagtggtt
     3961 ttgaaaaatt ctctaaagtg gaatttgtta caataacctt gggagaaagc attctgataa
     4021 aatccatact ttctttttgc ttcataaaga ctttgggaaa atagatcaca gtattttgga
     4081 ttttatctct attaaattcg aaaagtctag ttatagcatt tacatcagtt ataccttccc
     4141 ctataattaa acctacttct ttgaaggtca acctcaccaa aattggatag ggcaaagatg
     4201 aatctcttat gttaaattca ccttcgggtt taagcaattc gatctctaca ccttctatat
     4261 caagggtttc tgtttcttca tccaatatgt ttctccaaac gatgtcttta tctttaatta
     4321 tcccccaaag ctcaccatca agttttcttc cgttcgtcca gatactttct accttaaatt
     4381 tttgcagtat gtttttaacg ccttctaaat gatctttatc tagcgatgtc aaaatcagat
     4441 agttgatatt cgttacacct gacttaagaa gataaggggt cacaacgctc tgttcaataa
     4501 aacctcccat attaactttt gaaaatccgc catctattaa aattgttttt tctatgggaa
     4561 gccttatgaa tgttatgttt ctgctgccgg catcaaggaa gtttacttcg agaaaaccaa
     4621 ggtttggatt tcttaaactg gtgaacgccc atgtgacaag aaataccaaa ataaaaacag
     4681 gaagtagaag tctcagtttg taatatttct tccaaaaaag aagaggaatt attgaaggta
     4741 tcagtactaa ccacgtttta tagcctggtg aagggacagt cagtgatgaa aggggaaatt
     4801 ttactaattc ttttgttcct aagattagta atttaataag aaaaacgtct ccataaagaa
     4861 agaccctagc aacaacttcg gatactagaa aggttagaaa ggtaataagc ccaagaggga
     4921 ctatcaaaaa ttcaacaaat ggaacagcaa ctaggtttgc aggtattgat acaatagaga
     4981 tagttccaaa tgtgtttata atgaagggta aagtagcaaa agacgcagca acggtggttt
     5041 ttacacttgt gaaaagtttg tctttaaatg tgtttaactt aaagggataa aatctatgca
     5101 ttagaagtat tccgagaact gaagcgaaag agagttgaaa cgaaagttca aaaagtgccc
     5161 agggatgcca taacaggatt ataaaagctg agacggcgag tgtatttaac ttattttcct
     5221 ctcttcctat aactattgag acgagataag ctacagccat tattgaggct cgtacaacag
     5281 gggtagcaaa tcctgcgaca aacatataaa taaaaacagg aaggattgtt agtgcggctg
     5341 taagtcttgg tacctggaac ctgagaagaa ggtattcaga gcgtttgagg atccatttta
     5401 ttattaggta aaaaacaaat gcaactgatg ccacatgaag ccctgagatc gaaaggagat
     5461 gagcaattcc agcctcagaa aattcatttc ttaactctgg tgctatccct ccttgatctc
     5521 caatcgtgat ggcattaagc acctcgcttt caggaaatgg ggatttcttt cttacaaaat
     5581 tagcaaactt gactctaagc ttgtctactg cgtgaacgaa ggaatttgag cccttacttt
     5641 taccaaaaga aataatccat tcctttcctg ggacaaatcc tctggcataa actccttgtc
     5701 tttcatagta gcgtctaata tcaaaactac ccgggttttg aaaacttcta ataggtcgga
     5761 gttttgcctc aattaccctt attctatcac cgtatgcaag tcctgtgatt gtttcctctg
     5821 tagtaataat tacttttcca gaagaaggct tttcttttcc tcctataaga atcctatcaa
     5881 catcaataaa taatctcgat cccttctctc tgctttcagg ggatttatag agtacaccct
     5941 ctatatcaag ttccttcccc acaaagttca aaacattatt ttcttggagg tacggttttg
     6001 cagaaaatag aaggccaatt ggaataaaga ttaagaaacc taatttaggt ttgattaaat
     6061 atatagcacc aaaaacagga gagatgatgg taataagtat ccattcgttt ccaaaatact
     6121 cgctaagtag tattcctagg ataaaccctg aaagcggagg aaggatttta tatttcgaga
     6181 gaaaggaatt cattaatcaa cggtcttaat aagaacaaca gcataggctg ctattccttc
     6241 acccctgcct gtaaatccca taccgtctgt tgtagttgct ttaataccta gagatttctc
     6301 tggaactccg attatagaag caagcttctt ttttatatga gaaacatgcc cggaaatcct
     6361 tggtttttca cagacgacaa cagtatctat gttttcgacc ataaatcctt tggaatacga
     6421 taatgttaag acctccctta gtagaactaa actcgatata cctttatatt gggaattggt
     6481 atctggaaaa tgactcccga tgtctctttc tcccatggct cctagtaaag catcacagat
     6541 agcatgagtt aagacatccg catctgagtg gccttctagt cccagttcaa aagggatctc
     6601 taccccccct ataatgagtt tttgtccttg tgagaattta tgagcgtcga acccgatgcc
     6661 tacacggtgc attgattttc cattatgaat acactcaacg atttgagatt ccagaataag
     6721 gtgttcttag ccgaagatcg cacttagcct ttgttttgtg cgtgagagaa tagtaagtag
     6781 gtttgattct acgattaata tctttcactc tgtgctatat cctctccgtg gtcatatcgg
     6841 tcattatcta atagtcttgt tccttcctta agcactaact ctccgaggag taggtcctcc
     6901 ttggttgtta tctttatgtt tgtctgggag ccagcaacaa gcgtgacact tatgcctatt
     6961 ctctcaacaa gcgaggattc atcagtgcct aggaatccgt cgttttctgt cttttcataa
     7021 gccgatttta gaacattgta ctgaaaagcc tgtggtgttt gagcaagcca tagtgcctcg
     7081 cgtgaaaggg tccttacaac gtgattttta ttagaagatt tctttaccgt atccttaact
     7141 ggtatagcag cgattgcccc tcctgtattt aaagattctt ttataacctc atcaatcatc
     7201 cttgctgtta caaatgggcg tactccgtca tgtactacta ctacgtccgt ttcactcgag
     7261 acagatataa atcccatttt tactgattgc tgtctttgct tcccacccgg gatgattttt
     7321 gtgatctttt taaattgaaa cttatctttg atttctcttc gacagtagtt gattgaatcc
     7381 tttggaacaa caaggatgat ttcatttatt aacttggaat cttcaaatct ctcgattgaa
     7441 tatacaataa gcggctttcc agagagcggt tgaaactgct tccttaaacc ttcaccgaat
     7501 ctttttccga gtccagcggc tgctattatt gcagctactt ttagattaaa catggagatc
     7561 agagattaaa ctctcttctt taacccttga gaaaatcatc ctccctgtag gggtctgaag
     7621 aacactcgta acagagacct cgatttctct tccaacgtat ttccttgcat cgtcaactac
     7681 aaccatagtt ccatcttcga gataacctac accttgattt tcttccttac cctctttaac
     7741 aacgcgtata ttcatagtct cacctggaag cactatgggt ttaagtgcgc ttgcgagttg
     7801 attgaggtta agcacccgta caccgtgaag ccctgcgact ttattcaaat tgaaatcgtt
     7861 cgtaatgata attcctctta gactctttgc gagttcgacg agttttaaat ccgcctctcg
     7921 tacccttgga aaatcatggc ttgtgatttt aatcacaatg ttaggaatct cttgcatgcg
     7981 tttgagtaca tctagtcctc ttctacctct tacgcgttta attgggtcag gagagtctgc
     8041 tatatattga agctccttaa taatgaattt tggtacaatc ataatccctt cgataaatcc
     8101 tgtatcagca acatctgcta ttcttccgtc tattattaca ctagtatcca gtatctttgg
     8161 agtcctatga ttattcttgg ctgaggtttt tcttgctgtt tgatctaatt caaatccctt
     8221 ttttgctcca attgtaattc caatataaaa aaaggaaaga aaaataatag gtagtacata
     8281 cggagtaata ttagaaggaa tccttaaact atcaaacact attttaatcg caaagaagga
     8341 tagtgaggca acacaaattc ctatgcctcc tccaagtatt gctcttgggg taaattcatt
     8401 catccttgac tcaagccccc atataactac aaaagcaata gcaccaagga tacatcctag
     8461 tgcaattgca attataaagc tatagaaagg gtatgcaatc caaaacccca gggctgcggc
     8521 caagatcaag aaaagtattt taggtttcat ttcttcctca ccccctttct ctactcccct
     8581 ttcttttgga ttggattaaa tataagtcat aatgcgcgtt ttcacaagat tcgtggatcc
     8641 tttttctttt tcagcttttt gaattagaga tatgtttttt tgatacatcg ctccttagca
     8701 tttcccatag aaattatcca gagagacaac ttatcgaatt gcaaagagca atgcaaaagt
     8761 aggggattgc aattaatcta gaagtgttat gagctttctt tacccaggtt tgaaatgagt
     8821 ctgacaattt catccactac tcttgtatat ctacttgaac cagttccagg gtttttggct
     8881 cttccctcca tctctgcatc tgctatcaca tcacctgtgg ttttatcaat taactttact
     8941 ctgacagata ccgagctttt ccccttgtca ttgattctac cagctaaaaa ctcacaaaat
     9001 ttacaaccag gcttataacc agttacagtt cctttaacaa ggagcacact ttctcttgat
     9061 gctatattgg attcagagcg ggtaatctca ttaaaatgat tggatgttct tagtttctca
     9121 gctaccatat ctggaattag tgaagctgag tcatagggaa cccattcctt atcgttttta
     9181 ttgaaattgg gtatttcgat gatttggtag tttgcaattt tga
//
LOCUS       DATDRL010000244         2657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3461518, whole genome shotgun sequence.
ACCESSION   DATDRL010000244 DATDRL010000000
VERSION     DATDRL010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3461518"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VI935_08335"
     CDS             <1..412
                     /locus_tag="VI935_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25642.1"
                     /translation="KEEIKVSDFTENFKSLTGLVKENYLASLKLFLSLWEDNLKFANA
                     QFDQFLTTQKEYNEQLKDVFEKFSGQTTGLWNGNHQKAFDGAFDRIAGVQRDYAQLVR
                     SASEKFAKETVSLSQKTADKAFSFFEEYLNLLKV"
     gene            912..1286
                     /locus_tag="VI935_08340"
     CDS             912..1286
                     /locus_tag="VI935_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25643.1"
                     /translation="MPEISKDEESCYFSYFKILYESWERSMSKAMGVWFHNPIFTCST
                     IKAIEKSVEFKDYIYDIMDRTLKHRFIPAMHDMDKLVKSLDKLEVKVNKVEEKINKIQ
                     STKKLTTELKKGAQKKGGRNDE"
     gene            1279..1743
                     /locus_tag="VI935_08345"
     CDS             1279..1743
                     /locus_tag="VI935_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HLE25644.1"
                     /translation="MSEEKFDYMDYYKKFYEVWEKSMSEALEMWSKSPLLNKDVAAGN
                     DKTMDFDPALYYKRFYETWEKSMSEALEMWLKSPLFAASAGKLIEKSSGLKKYLDEVM
                     ERSLNNMRLPTKNDIDRVLASLNNLEAKINDIWDKIDELKTGGRATTKKSSS"
     gene            1903..>2657
                     /locus_tag="VI935_08350"
     CDS             1903..>2657
                     /locus_tag="VI935_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLE25645.1"
                     /translation="MATNYLESLMQQTEKLREKFIKGVELALTNPPEIKLGITPHEVI
                     FTENKLRLLHYYPVVEKPHPVPLILIFALINRPYILDLKPGKSVVEVLLKKGLDVYLI
                     DWGTPGDEDKHLNLNHYINRYIKKVVEKVKKISGSDKVSLLGYCMGGTMSAMYTALYP
                     EGVQNLILMTAGIDFKVDGGMLNLWGDKPHFDVDKFIDAYGNAPPEFLQTGFLIMKPI
                     QNMFSKYVSFYENIDNEDFVENFLAMEKWINDN"
BASE COUNT          943 a          415 c          536 g          763 t
ORIGIN      
        1 gaaagaggag ataaaagtct ctgattttac agagaatttt aagagcctta caggcttagt
       61 gaaagaaaat tatcttgcaa gccttaagct tttcctttct ttatgggaag ataacctgaa
      121 gtttgcaaat gctcaatttg atcagttcct tactactcag aaagagtata atgagcaatt
      181 aaaggatgtc tttgagaaat tctcaggcca aactactggc ctttggaatg gcaaccatca
      241 gaaagcattt gacggggcct ttgaccgcat agcaggtgta cagagggatt acgcacagct
      301 tgtaagaagc gcctccgaga agtttgcaaa ggagactgtg agtcttagtc aaaaaacagc
      361 agacaaggct ttttcgttct tcgaggaata ccttaacctc ttaaaagtct aagccttaat
      421 ataaacaggt agatttattc agacacactc tcagattagg gcgtgtctta aatcaacaga
      481 tctaattccc aactttatat atcaaagggg tgtaatttgc gaaccatata agtgtttggt
      541 ttgggattat aactaacatt cgttataaaa ttacacttta gtaaatttga tggggcctag
      601 agatgcaaaa aatcgcctga attttataag cgggaagaac gtagcaaaaa agcgttcttg
      661 cccaataaga ttgctttgga aacaatttct caatgtgctt cgtataaacc agtttacaaa
      721 aaacttaaat aaaatttgaa tatagatgtg gttgagtgat atgtttaagc atcgtaagtt
      781 tataagcttt gatttacaag attgctctta ggttagaaat acggctttag ataaaagaaa
      841 acaaccctta tgttgctaac ccttaattat ttaaatatca tgatagatac taattgttag
      901 gagagtggcc gatgcctgag ataagcaagg atgaggagtc atgctatttt agttatttta
      961 agatactcta tgaatcatgg gaaagatcca tgtctaaagc tatgggggtc tggttccata
     1021 acccaatttt tacttgtagc acaataaaag ctatagaaaa atcagtagag tttaaagatt
     1081 acatatatga catcatggat agaaccctaa aacacagatt tattccagcg atgcatgata
     1141 tggacaagct agtcaagtcc ctggataaat tagaagtcaa agtcaacaag gtcgaagaaa
     1201 aaattaataa aattcaaagt acgaagaaat taaccacaga actaaaaaag ggagcacaaa
     1261 aaaagggagg tagaaacgat gagtgaagaa aagtttgact acatggatta ttacaaaaag
     1321 ttttatgaag tgtgggaaaa gtctatgtct gaagcactgg agatgtggtc aaagagccca
     1381 ctactaaata aagacgtggc ggcaggtaat gataaaacaa tggatttcga tccagctttg
     1441 tattacaaaa ggttttatga aacctgggaa aaaagtatgt ctgaggccct ggagatgtgg
     1501 cttaaaagtc ctttattcgc cgcaagtgct gggaaattaa ttgagaaatc ctcagggctt
     1561 aaaaaatacc ttgatgaagt catggagaga agccttaata acatgcgtct cccaacaaaa
     1621 aacgatattg atagggtttt ggcatcactt aacaacctag aagccaaaat aaatgacatt
     1681 tgggacaaga ttgatgaatt aaaaacagga ggacgtgcta caactaagaa gtcttcttct
     1741 tagggttaaa tttatatttg attactaatt tgtcatttct tatgtggcaa gtttctagaa
     1801 atataatgat taataaatgt cgtgagaata ccgaattaga acacaggatt gcattcattt
     1861 gacttgatgt taatattaaa tattaagagg ttatgagata gaatggcgac aaattacctt
     1921 gaatccttga tgcagcaaac agaaaagttg agagaaaaat tcataaaggg cgtggaatta
     1981 gctttaacaa atccgcctga gattaaacta ggcataactc ctcatgaggt tatctttaca
     2041 gaaaataaac ttaggcttct tcattattat cctgtagtag aaaaacccca tcctgttcct
     2101 cttattttga tttttgcact aataaatcgc ccttacatac tcgacctaaa acctggaaag
     2161 agcgttgttg aagtactcct aaaaaaggga ttagacgtat atcttattga ttggggaacc
     2221 cctggggatg aagataagca tctaaatcta aaccattata ttaaccgtta tataaaaaag
     2281 gtagttgaaa aggtcaaaaa aatatcgggt tcagacaagg tgagtcttct tgggtactgc
     2341 atgggtggca caatgtccgc tatgtacact gcgctttatc cagagggtgt gcaaaaccta
     2401 attcttatga ccgcaggaat tgatttcaag gttgatggag gcatgttaaa cctttgggga
     2461 gacaaacctc atttcgatgt tgacaaattc attgatgcat acgggaatgc tcctccagaa
     2521 ttcttacaga caggatttct tataatgaaa ccgatacaga acatgttttc taaatatgta
     2581 agcttttatg aaaatataga taatgaagac tttgttgaga acttccttgc catggaaaaa
     2641 tggataaacg ataatat
//
LOCUS       DATDRL010000245         5064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1974196, whole genome shotgun sequence.
ACCESSION   DATDRL010000245 DATDRL010000000
VERSION     DATDRL010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1974196"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..629)
                     /locus_tag="VI935_08355"
     CDS             complement(216..629)
                     /locus_tag="VI935_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25646.1"
                     /translation="MAERKETLKVKDFSEVIKGVTGLVRENYQNGFEFALSLWEENLK
                     VLNAQVDQWLNLQQDYTNTLRDLYEKLPKEVPAFWDGKAINSEVNRLLAFQKEYLGLV
                     RNVSDKLTKETLSLTKKNIEKAISLFDDYINLVKI"
     gene            complement(1043..1258)
                     /locus_tag="VI935_08360"
     CDS             complement(1043..1258)
                     /locus_tag="VI935_08360"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HLE25647.1"
                     /translation="MGKSNLTLSILAPNPVKNGKEIEKSTPLSGSLTDKIYKYTIQVT
                     AFEGMEKAQRVVNELKEKDYDAYSVCF"
     gene            complement(1240..1470)
                     /locus_tag="VI935_08365"
     CDS             complement(1240..1470)
                     /locus_tag="VI935_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25648.1"
                     /translation="MGELIPKLVLAKPKRGGRFKLILILLVTFGIGVFVGTAIDTVNI
                     LERDASTSKEAKVSNPGIDTKEKVSRIWENLT"
     gene            complement(1543..1630)
                     /locus_tag="VI935_08370"
     tRNA            complement(1543..1630)
                     /locus_tag="VI935_08370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1593..1595),aa:Leu,seq:cag)
     gene            1953..3035
                     /gene="amrS"
                     /locus_tag="VI935_08375"
     CDS             1953..3035
                     /gene="amrS"
                     /locus_tag="VI935_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HLE25649.1"
                     /translation="MAIKKELKTLARPSLAEVLDEYSVEGELYQKLENNKVLCYACGH
                     RCVIYDGLRGICKVRYNKEGKLFVPKGYVAALQSDPTEKKPFFHVLPGSFTLTFGMLG
                     CDYHCSYCQNWLTSQALRDSTAGIEPMTVTATEMVSLAKRYGASMIGSSYNEPLITSE
                     WALDVFRIAREQGFKNAFISNGNATREVLEYLKPLTDCYKVDLKSMQDRNYRKLGGLL
                     STILETIPRLVEMGFWVEIVTLVIPGFNDSNEELKEAAKFLNSISPDIPWHITAFHKD
                     YKMTDPDNTSPETLIRATEIGLEAGLRFVYAGNLPGWVKNYENTYCPNCHKLLVERYG
                     YRILNYSITPDGKCPSCSSTIPGIWW"
     gene            3851..4138
                     /locus_tag="VI935_08380"
     CDS             3851..4138
                     /locus_tag="VI935_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HLE25650.1"
                     /translation="MIAKVSSKGQVVVPKEIRKEMKIKAGTLFEFRQIDKKRLEITVI
                     HDLIKELEGIYKGSGMLKELEREHKEEIERDELYSRRLGGRSMARKRKRVP"
     gene            4074..4475
                     /locus_tag="VI935_08385"
     CDS             4074..4475
                     /locus_tag="VI935_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HLE25651.1"
                     /translation="MNYILDAWAVVAWLEKESGYLRVSELLEKAGDREIRLTMSIVNF
                     GEVFYRTGKLYGIAEATRTERRIRALPINIIAPVEETLVMHAAYLKAQHPITYADAFA
                     AALAEQEKAILVTGDRDFKRVEKKIKIEWLR"
     gene            4683..>5064
                     /locus_tag="VI935_08390"
     CDS             4683..>5064
                     /locus_tag="VI935_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLE25652.1"
                     /translation="MEPKYIRNFSIIAHVDHGKSTLADRILEFTGSVTEREMTEQFLD
                     QMELERERGITIKAQAVRINYKADDGITYEFNLIDTPGHVDFSYEVSRSLYACEGVLL
                     VVDASQGVEAQTLAHAYLASDSGLE"
BASE COUNT         1518 a         1036 c          953 g         1557 t
ORIGIN      
        1 tcatcatctt catgaacaaa ctctccaagt tcgtttactc cagaagttgt ttcttcaaac
       61 tccatgtcct ttatctccat ttcttcatca ttccttcatt ggattattta aataccatta
      121 acaaagaggt aaagaaaaat aatcttatta gtagggttct gagtaaaccc aattaattct
      181 tttgtctata ttatattcac aggaatctat aatatttaaa tttttaccaa gttaatataa
      241 tcatcaaaaa gcgatatagc cttctcgata ttctttttag taagactcaa agtttccttt
      301 gtaagcttat ctgaaacatt ccttactaag ccgagatatt ctttttgaaa agccagaagg
      361 cgattaacct cactatttat agcctttcca tcccaaaatg ccggcacttc cttcggtagc
      421 ttttcataga gatccctcag agtattggtg taatcctgct gaagattaag ccactgatca
      481 acctgagcgt ttaaaacctt aagattttct tcccaaagcg ataaagcaaa ctcaaagcca
      541 ttttgatagt tttctctaac caatcctgta acccccttta ttacctcact aaaatccttt
      601 acctttagtg tttccttcct ttctgccata attcattttc ctccaattta atttttttat
      661 gtggttaata taatgtatgc tagatcattt gtcaagaggt tttttcgcag ttgcgaggaa
      721 tcattttatt agagattcat agaataatag gcgaaaactc atacatccac ttttattatt
      781 acgcataagc taacaaaatt gagcaattac actgtaaata ttataaaatt taaaagccca
      841 aacttaagac ctagtatttt tacttagttt tgcatgaaaa tttgaataca acatgttgct
      901 agatgaaaat tattccaact ttagttcttc tataacgggc tccgaggctt ctttttgttg
      961 taataggtca gcaggcgctt gggcctcctc ttgagtctag aacttgccta cctttataag
     1021 cttccaggct tcccctagtg aattagaaac agacactata agcatcataa tccttctcct
     1081 ttagttcatt tacaactctt tgtgcttttt ccatcccttc aaacgcagtt acctgaattg
     1141 tgtatttata gattttatca gttaaagatc cgctcaaagg tgtggatttc tctatctcct
     1201 ttccattttt cactggattt ggagcaagta ttgatagagt taagttagat tttcccaaat
     1261 ccgactcacc ttttctttag tatcgatccc cggattacta acctttgcct ctttactcgt
     1321 ggaagcgtct ctctccaata tatttacagt gtctatcgca gttccgacaa agacaccgat
     1381 accaaaggta acaagaagta ttagaatgag tttgaatctt ccacctcgtt taggttttgc
     1441 caaaactagc ttaggtatta gttcacccat tgatactatt cacttttaat ttccatttga
     1501 tgcacactgc gcatcaaata atacagaatc accttcccca catggtgccg aaggggggat
     1561 ttgaaccccc acgagctttc acccactgga tcctgaatcc agcgcgtcta ccaaattccg
     1621 ccacttcggc atcaaaaaat ctaatgaatt ttggtagcta taacttacct ccaaaaaatg
     1681 caatttcatt atttgaaaaa atttcgtctt acatgctacg taatttatct tgaattttga
     1741 ttccgatctt aaagtgtaat accattaaaa tatctcgtca aacccatact taagaccgtt
     1801 aggtttataa aaatcctaca cactaactcg gttttcctta cattttatag tcttagaaag
     1861 tttgacacta tatacttaaa agcataaact ttattgtaaa gtctcatcct gtggtgagac
     1921 catctaaata tgtttaattg gggaaatgag gtatggcaat aaaaaaagaa cttaagacac
     1981 ttgcaagacc ctcccttgca gaagtactgg atgagtattc tgtagagggt gagctttatc
     2041 aaaaattaga aaataataag gttctctgct atgcatgcgg ccatagatgc gttatatatg
     2101 atgggcttcg tggaatatgc aaagtaagat acaataagga aggcaagctt tttgtcccaa
     2161 agggatacgt agctgcgctc cagtctgatc ccacagagaa gaagcctttc ttccatgttc
     2221 ttcctggttc tttcacacta actttcggga tgttaggctg tgattatcat tgttcatatt
     2281 gtcagaactg gctcacatcc caggctctta gggattcaac agcgggtata gagcctatga
     2341 ctgttactgc aacggaaatg gtttcccttg caaaaaggta tggagctagc atgattggta
     2401 gctcttataa cgaaccactt attacttctg aatgggcatt agatgttttt cggattgcta
     2461 gggaacaggg atttaaaaat gcttttatct caaacggaaa tgcgacccgt gaggtcttag
     2521 aatatcttaa acctctcact gactgctaca aggtagacct aaaatccatg caggatagaa
     2581 actatcgaaa actcggcggt ctccttagta caattcttga aactattccc cgattggtcg
     2641 agatggggtt ttgggtagag attgttacat tggttattcc tggatttaat gactcaaacg
     2701 aagaattaaa agaagctgca aaattcctta attccatatc gccagatatt ccctggcata
     2761 tcacagcgtt tcacaaggat tacaagatga ccgaccctga taacacctcg cctgaaacct
     2821 taattcgcgc caccgaaatc gggcttgaag caggacttag gtttgtttat gccggaaacc
     2881 ttcccggatg ggtaaaaaac tacgagaata catactgccc aaactgccat aaactcctgg
     2941 ttgagcgata tggatacagg attctaaatt actctatcac tcccgacggc aaatgcccca
     3001 gttgctcttc cactatcccg gggatttggt ggtgagatca ttaggtcaag ttgtagtcct
     3061 atatcatctc gtaccccacc cttgtccaat tatcgttttg gtggatactt aaatagcaac
     3121 accacaaatc ccttggttta ccttgatcat tttccgctta taaatggtta gtgctcttac
     3181 caacggatct ccacaatcca aggtactgat aaaagaatca tcgtatctcg ccctattcct
     3241 cgggtctctc gtacaaagca cgggagttcc aagtgatgat agatgggcgc gtgtttagta
     3301 ataaggtagc tttttgcgaa agggaaaacc caaaacgaca tccggtttaa tgattgagta
     3361 caagactgat gattgacttg gtgcaccctt tcctgcttac tgccagtttt gatacattta
     3421 ttgaatgtag taacatagtt taattacctt tcactcggct atttaagtgt aaacctcccc
     3481 tttagcacaa ctatctaacc aataatatcc accgtcatcc aaatttttat tcgaatttac
     3541 tcatctaggg agttaacaac aacctccttt tgccatttgt ttgtttcctt tttttagggt
     3601 taataataaa cctcagattg ggattttata taggacgttc ctggacggtc acgagcaaga
     3661 ctcgtgatct acccaatgga cagaaataaa aaaccaggta gtctgctttc ttcttaagtg
     3721 tggtagtaca ccagtctatt aattagggta gcccgccagt ctggctggtg ggcggtcaca
     3781 gggtccctcg atttgactat taattttgtc ttacctataa ttaatagtaa gacaactatg
     3841 aggaggtaag atgatcgcaa aggtttcatc aaaaggacaa gtcgtagttc caaaagagat
     3901 aaggaaagaa atgaaaatca aagccggcac tttgtttgaa tttagacaga tagataagaa
     3961 gcgtcttgag ataaccgtaa tccatgatct gattaaggaa ttggaaggaa tttataaagg
     4021 gtcagggatg ttaaaagaat tggaacgaga gcataaagag gagattgaac gagatgaact
     4081 atattctcga cgcttgggcg gtcgtagcat ggctagaaaa agaaagcggg taccttagag
     4141 ttagtgaatt gcttgagaag gcaggcgata gagagataag gctgactatg agtatagtaa
     4201 attttggtga ggttttttac cgcaccggga aattgtatgg aatcgcagag gcaacgagaa
     4261 ctgaaaggag aataagggct cttccaataa acataattgc acctgtggaa gaaacgctcg
     4321 ttatgcatgc cgcatacctc aaggcgcagc acccaatcac ctatgccgat gcttttgcgg
     4381 cggcattagc tgaacaagaa aaagcaattc ttgttactgg tgaccgggat tttaagcgag
     4441 ttgagaaaaa gataaagata gagtggttaa gatgacggtg aaacataaca attaagaagt
     4501 cacgagccaa gagtctctta ttttcttctg gattacgaat tctggcttct gtattctaac
     4561 ccccatctac tttaaaactc atattctcca ttgtgcctta gtggctaaac ggttcatttg
     4621 cttaaccccc cttttccctt ataatatcat ttcacagtag tcttaaagat cgaacacata
     4681 gcatggaacc taaatatata aggaactttt ctatcatcgc ccatgtggat catggaaaat
     4741 caacgcttgc agatagaatc ctggaattca ctggaagtgt aaccgaacgc gagatgaccg
     4801 agcagtttct cgaccagatg gagttagaga gagagcgcgg gataacgatc aaggctcagg
     4861 ctgtgagaat caactacaaa gcagacgatg gaatcactta cgaatttaat ttaattgata
     4921 cacctggtca tgttgatttc agttacgagg tctcgaggag cctttatgct tgtgagggtg
     4981 tgcttctcgt ggttgatgca tctcaaggtg tcgaagctca aacacttgcg cacgcttatc
     5041 ttgcatcaga ctcaggactc gaac
//
LOCUS       DATDRL010000246         3408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2019364, whole genome shotgun sequence.
ACCESSION   DATDRL010000246 DATDRL010000000
VERSION     DATDRL010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2019364"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..935
                     /locus_tag="VI935_08395"
     CDS             321..935
                     /locus_tag="VI935_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25653.1"
                     /translation="MLDEYETRKKTLEKAIQIAKREWGNIKSSLMYCGDIGEFCKEDF
                     MIGVIEEDVIIREPLISPTKSVSGYAPTFYPMYLTQNLIIMDEKISKYSYKTIEALYV
                     FIELATKAAERLGLVGTFSIGFGSGYGYVRTGWIGEKGRPEERDIFYKMFYKGRVDYD
                     WDFHWTSVRQRMNLIFTRFMAWQNNPKLYESEVKPSAKVKPMMV"
     gene            1152..2471
                     /locus_tag="VI935_08400"
     CDS             1152..2471
                     /locus_tag="VI935_08400"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLE25654.1"
                     /translation="MIQDTIVIPTRKEIFGWAMYDFANSAFATTISSVVFNVYFTKVI
                     VGDGGAEIFGHNIPASSLWGYTVSLSILLVVFTAPILGAIADFTGTKKRFLFLYCYLA
                     CLFTGLLFFAEEGDYIFAMIFYMLANYCFAGSLGFYNAFLPEISTKENIGRISGLGWA
                     IGFVGGGILLAINLVMIRNPQLFGIPEEDNLPVRFVILSVAIWWAVFSVFTFLWVREK
                     TKGNPLPRGENYVSIGFKRLVLTFKKIRQHKELFKFLVTFLIYNEGIETVIVMAAIFG
                     SEILGMSQSEIVTLFLLGQIVAFCGSLLLGYLADKYGNKKAIILSLIIWCVLVIWAYF
                     MKTRFEFWTIGVFIAFILGGSQAVSRSLFGLFTPRENTAEFFGFYALGGKFSSAIGPF
                     TFAFIRHAFDSIRVAIIALIVFFSLGLLFILFVNERRGIEESSKPIG"
     gene            complement(2716..>3408)
                     /locus_tag="VI935_08405"
     CDS             complement(2716..>3408)
                     /locus_tag="VI935_08405"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HLE25655.1"
                     /translation="DALSIRAVEEVIHAGYPLDAEAVLLIELEGLNEILSRSFPLVKD
                     FCIKNRARDVRIAETEIERERLWKGRKSVAGAIGKIAPNQYTQDGVVPRPKLPEVLRK
                     VIEIGKNRSLLIANVAHAGDGNLHPLILFDPRHPGELERVIEAGGEILKLCVDAGGTI
                     TGEHGVGIEKNKYMSWLFSEADLKAMEIIKDTFDPKGIFNPGKIFPTPKGCRELRVQP
                     IGVRTSEKGIWI"
BASE COUNT          963 a          632 c          750 g         1063 t
ORIGIN      
        1 agaatacaac ggtagccagt gggaaaagcg gtataacacc tgagaagttt tgaaggtttg
       61 aaacgttcgc taggtcaata gtaaaattct tcattatagt agtattttac caaataaggt
      121 gttgaacttg gagtatctaa tttagttgag ggaatttatg taaatgcact acagaaaagt
      181 aaacataacc cggtattaga tcgacgtagt cttgataata aaaaattgtt gtgacataag
      241 atcctattat cattttgata atccaagcct aggtatgtat atttaaaacg ctcaaatccc
      301 aatcgtgtat aggttgctaa atgcttgatg aatacgagac aagaaagaaa accctagaaa
      361 aggctattca gattgcaaaa agggagtggg gtaatattaa atcatccctc atgtattgtg
      421 gcgatattgg agagttttgt aaagaagatt ttatgatagg agttatagaa gaggacgtga
      481 taatcagaga accactaata agtcctacaa aatcagtttc aggatatgcc cctacattct
      541 atccgatgta tcttacacaa aacctaataa tcatggatga gaagatctct aagtattcgt
      601 ataaaaccat agaagctctt tacgttttta ttgaacttgc cacaaaagcc gcagagaggc
      661 ttggtcttgt aggaacattc agcattggat tcggaagtgg atatggttat gtccgtaccg
      721 gttggatagg agagaaaggg agacctgaag aaagggacat cttctacaag atgttttata
      781 aagggcgagt tgactacgat tgggattttc actggacttc tgtaagacag agaatgaacc
      841 taatcttcac taggttcatg gcttggcaga acaacccaaa gctttatgaa agtgaggtaa
      901 aaccaagtgc caaggttaaa cctatgatgg tttaaaaaat gaacctaggt tgtgatctaa
      961 gtacagatgt cattgcgagc gactaaaagg gtgagccaga gccccgaaca acgtgagagg
     1021 ggaagcaatt tggtacagaa tgtcattccg aatactgcgg agggctcctc gcaatgacat
     1081 tacctttttt atagggagca aatttgttta aacagattcg caaactagac tgcatttttg
     1141 gttaaataac attgattcag gatactatcg ttattcccac cagaaaggag atattcggtt
     1201 gggcgatgta cgattttgca aactcagcgt ttgcaacaac tataagctct gtagtattta
     1261 atgtgtattt tacaaaagtt atagttggag atggtggcgc agagatattt ggccacaaca
     1321 tacctgctag ctctctttgg ggttatacag tatctttatc aatccttctt gttgttttta
     1381 ctgcacccat ccttggagca atagccgatt ttacgggtac aaagaaacga tttctttttc
     1441 tgtattgtta tcttgcctgt ctttttacag gactcttatt tttcgcagaa gagggtgact
     1501 acattttcgc tatgattttc tatatgcttg cgaactactg ctttgcagga agtctgggtt
     1561 tctataacgc gtttcttcct gagatttcca caaaagagaa tataggtagg atttcaggtc
     1621 ttggatgggc tatcgggttt gtaggtggag gaatccttct tgccataaac ctagtcatga
     1681 ttcgaaaccc acaacttttt ggaatccctg aggaagacaa tctgccagtt cgttttgtta
     1741 ttctctctgt cgcaatctgg tgggctgtgt tttcggtttt tacctttcta tgggttcgag
     1801 agaaaacaaa ggggaatccg ctgccaaggg gtgagaacta tgtgagcata gggttcaagc
     1861 ggcttgtgct tacctttaaa aagattaggc aacataagga gctctttaaa tttttagtca
     1921 cctttctgat ctacaatgag ggcattgaga ccgtaatagt gatggctgct atttttggaa
     1981 gcgagattct ggggatgtct cagtctgaga ttgtaacctt attccttttg ggacaaattg
     2041 tagccttttg cggttcttta ttattgggtt atttggctga taaatacggc aacaaaaaag
     2101 cgattatact tagtttaatt atttggtgtg tccttgtaat atgggcttat tttatgaaga
     2161 cgaggtttga attctggacg ataggggtgt ttatagcttt tatattagga ggcagccagg
     2221 cagtaagccg ttctctcttt gggcttttta ctccgcgtga gaacacagcg gagttctttg
     2281 gattttatgc acttggtggc aaattttctt ctgcaatagg cccatttaca ttcgcattta
     2341 tacgccatgc gtttgactca attcgtgttg ccattatagc gcttatagtg tttttttcgc
     2401 ttggcttgct atttatcctt tttgtgaacg agagacgagg tatagaagaa agcagtaagc
     2461 caatagggtg agtcgcagcg aaaagcaaac acgcccttcg acgggggtga acggtatttg
     2521 aaatatgcca tttgtggttg accctgagct cagtcgaagg gtcgaaccat gaacagcgat
     2581 tctgccttac tagcgggatg acattttttc catcagggaa tactaaaggg tctaactaca
     2641 attatatacc cttacgtcga aattacattg aattttttga attagaaaga acgataggga
     2701 tcctattgct aattgttaaa tccagatccc cttttcacta gttcggacac caataggctg
     2761 gacgcgtagt tctctgcagc cctttggagt tgggaaaatt ttgccaggat taaaaattcc
     2821 tttggggtca aatgtatctt tgattatctc catcgcctta agatcagcct cgctaaaaag
     2881 ccagctcatg tatttgtttt tctctattcc tacaccatgt tcaccagtga ttgttcctcc
     2941 agcatctaca caaagtttta aaatttcacc gccagcttca ataacccttt ccaattcacc
     3001 tgggtgtctt ggatcaaaaa ggattaatgg atgaaggttg ccgtccccgg cgtgtgcaac
     3061 gttggcgata agaagtgaac gattctttcc tatttctatt acctttctca gcacctctgg
     3121 aagcttagga cgtggaacta ctccgtcctg ggtgtattga tttggtgcta ttttgcctat
     3181 tgcacctgca acgctttttc ttcccttcca gagacgctcc ctttcaatct ctgtctcagc
     3241 gattcttaca tcacgtgcac gattcttaat acaaaaatcc tttactaaag gaaaagacct
     3301 actgagaatt tcgttaagcc cttcgagttc gataagtagc acagcttccg catcaagagg
     3361 gtatcccgcg tgaataactt cttcaacagc gcgaatagag agcgcatc
//
LOCUS       DATDRL010000247         5558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3567430, whole genome shotgun sequence.
ACCESSION   DATDRL010000247 DATDRL010000000
VERSION     DATDRL010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3567430"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VI935_08410"
     CDS             <1..534
                     /locus_tag="VI935_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25656.1"
                     /translation="TTILSVFVRRYLHLQDYITKSQFQDLGKLIFGFSLFWTYLFFSQ
                     YLAIWYGNMPEETGFVFNRVREEPFKTLSWVVISCCFFFPLVTLLPKTNKITTPIIAF
                     IATVSLSGLWLEKFVLVTPSLSPEIHFCWLQILITLGFFSAFMLTFLFFIKNFPILPV
                     GDPLFSGKIGTHRSGGH"
     gene            535..690
                     /locus_tag="VI935_08415"
     CDS             535..690
                     /locus_tag="VI935_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25657.1"
                     /translation="MEIEEIQESVDKITQLVVAILCISVAGGILTIAAIALFGPQMSP
                     LMQKLPF"
     gene            859..1875
                     /locus_tag="VI935_08420"
     CDS             859..1875
                     /locus_tag="VI935_08420"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HLE25658.1"
                     /translation="MMLRTRLYNLLRIEFPIICAPMGFVTGPELAAAVSNAGGLGIMP
                     FSGNPPSALRQEIRRLRSLTDKPFGVNLLLHYPVDEHVTVCIEERVPILSFFWGDPSR
                     FIDRAHEAGIKVFHQVGSVKGAQRAVRAGVDVVIAQGVEAGGHIEGVVTTMALVPRVV
                     DAISPIPVVAAGGIADPRGVVAALALGAEGVVLGTRFLATIESNAHPIYKQRVLAATE
                     EDTVRITLFGHGWPNAPHRTIRTAFVDEWLSKEDQGNESRSDEPVIGETRIGGQPVPV
                     VRFKGFPPSKDARGDIESMNLLAGQGVGLVNEIKPAATIVRELVEGAREIIQKRLDRI
                     LANK"
     gene            2001..2546
                     /locus_tag="VI935_08425"
     CDS             2001..2546
                     /locus_tag="VI935_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015157636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLE25659.1"
                     /translation="MRHRRSIRLKDYDYSQEGVYFATICIQNRQCLLGYVQDGTMFLS
                     HAGEMVKSVWMDLPNRFDHILLVEFVVMPNHIHGIIIIHRRGESCIRPNSSMGDQKDR
                     PYGTEPDSIGRIIQAFKSITTNKHIWGVREKKWPAFPNRLWQRNYYEHIVRNEKELHN
                     LREYVANNPTNWAEDEENPDK"
     gene            2597..2932
                     /gene="gldC"
                     /locus_tag="VI935_08430"
     CDS             2597..2932
                     /gene="gldC"
                     /locus_tag="VI935_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HLE25660.1"
                     /translation="MSKEVEIRFSIVLDDNNIPVAIFWEASDSEFEGKKPCDSVMISI
                     WDKKEKNALSIDLWTKDMIVGEMNAHFFLTLMKMAETYERATKNNEVANMIKGFANDF
                     ATKVEELTK"
     gene            complement(2951..3880)
                     /locus_tag="VI935_08435"
     CDS             complement(2951..3880)
                     /locus_tag="VI935_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HLE25661.1"
                     /translation="MKKVKFGVILRQEKIDFKTIRETAELCDELGYHSIWFYDHILGM
                     GSIDFDVFEAWTLMSALSTITSKVKIGTLVLCNSFRHPSLLAKMASTLDHISNGRLEF
                     AIGAGWFEPEYKAYGYPFPDTITRIEQLKEAVKIIRALWTQEKATFIGKHFQIRDAYC
                     NPKPIQKPHPPITIGGSGERHLLRVVAELADGWNCPASNAADYDRKLKVLKTHCKSLG
                     RDISELEISEQTVCVIAKDKTELEEKLPKAKKRFSFFGDIEKTGIVGTPDQCIEKITH
                     KVNKGVSKFTIFFYDILNPEFLRFFAKEVMSEF"
     gene            4049..4765
                     /locus_tag="VI935_08440"
     CDS             4049..4765
                     /locus_tag="VI935_08440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLE25662.1"
                     /translation="MTQVLLQSGFGLKVGYIIINKVPPVIMLIILFMLIWGCEQKEPK
                     QSEQKQNERKTVDQQGSENKRSHTQGGNPMVIMSTSVGDIKIELYSDKAPMTVNNFLA
                     YVNDKFYNGTIFHRVIPGFMIQGGGFTPEMQQKATKPPIKNEANNGLKNDTGTIAMAR
                     TSVVDSATSQFFINLTGNDFLNHGDRDFGYAVFGKVVEGMDVVNKIAGVKTGSHGMHR
                     DVPLDPVEVESIRVIEPVGK"
     gene            complement(4776..5261)
                     /locus_tag="VI935_08445"
     CDS             complement(4776..5261)
                     /locus_tag="VI935_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLE25663.1"
                     /translation="MTLIVRSLGIQDRGRIECILAEVKIFSSEEIKVALELIDSCLSG
                     NPDYIIKVATDEKGTVLGYICYGKIPLTDAVWDIYWVVISDEFQGKGIAKKLLTFMED
                     DLKAKSARAVMVETSSRPEYEPARSFYMRNGFIEVSRISDFYAKYNDKVIYRKDLNSK
                     N"
     gene            complement(5224..>5558)
                     /locus_tag="VI935_08450"
     CDS             complement(5224..>5558)
                     /locus_tag="VI935_08450"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25664.1"
                     /translation="NEYKNTITICPASLNKKTEKRIKDIALKASRTIGCRDYARVDMR
                     MDSLGKIYVIEVNPNPDISPESGFSKALKAAGISYKAFVNQVVGFAIQRKKARYYDTN
                     SKELGNTG"
BASE COUNT         1629 a         1096 c         1151 g         1682 t
ORIGIN      
        1 acgactattc tttcagtctt tgtacgaagg tatcttcacc tccaggacta tataacaaaa
       61 tcccagtttc aagatctcgg caagcttatt tttggatttt cgcttttctg gacctatctc
      121 ttcttttctc agtatcttgc aatatggtat ggtaacatgc ccgaagaaac ggggtttgtg
      181 tttaatcgag tacgtgaaga gccgttcaag acactttcat gggttgttat atcatgttgt
      241 ttcttctttc cacttgttac actacttccg aaaacgaaca agattactac cccaataatc
      301 gcattcatag ccactgtctc cctaagcggg ctatggcttg agaaatttgt tttggttaca
      361 ccatcccttt ctcccgagat ccatttttgc tggttgcaga ttcttattac ccttggtttc
      421 ttttcagcct ttatgctcac atttttattt ttcataaaaa acttcccaat tctgcccgtg
      481 ggtgatcctt tatttagcgg aaagattggg actcatcgtt ccggaggaca ttaaatggaa
      541 attgaagaaa tacaagaatc cgtagacaaa atcactcagc ttgttgttgc cattctctgt
      601 atcagcgttg caggtggaat actcacaata gccgctattg ctttgtttgg acctcaaatg
      661 tcaccactta tgcagaaact cccattttaa tacttaagta gaagccggtc aatgtatatt
      721 tagcctcaca gtgttaagtt tattactcga tattgcagaa ataaaaaaac aaatcaaaga
      781 ttttcttaaa ttgccttcct caacttcctt tatcataata acgctatcca ccattacggt
      841 aatctaaagg aggtaaagat tatgttgcgt actcgcctat ataaccttct tagaatagaa
      901 tttccgataa tttgcgctcc gatggggttc gttactgggc ctgagttagc ggcggctgtg
      961 agtaatgctg gtggtctagg catcatgcct tttagcggaa atccaccctc agctcttcgt
     1021 caagagattc gccgactaag aagcctcact gataagccgt ttggcgttaa cttacttcta
     1081 cattatcctg tggatgagca cgtaactgta tgtatagagg agcgagtacc aattttgtct
     1141 ttcttctggg gtgacccctc tagattcatt gatcgggcac acgaggcagg tataaaggtt
     1201 ttccatcagg tagggtcagt taagggagct cagcgcgcag ttagagcagg tgtggatgta
     1261 gtaatagccc agggagttga ggctgggggt cacattgagg gcgtagtgac tacaatggca
     1321 ctcgtgccac gcgtcgttga cgctatttcg ccaatcccag ttgttgcggc aggaggaatt
     1381 gccgatccac gaggagttgt tgcagcttta gcactgggcg cggagggggt ggtgctaggc
     1441 acacgatttc tcgcaacaat tgagtctaat gctcacccaa tctacaaaca gagggtgtta
     1501 gcagcgacag aggaggatac ggtgcgtata actctatttg gtcatgggtg gcctaatgct
     1561 ccacaccgta caatacgtac agcgttcgta gatgagtggc ttagtaagga ggatcagggt
     1621 aatgagtcac gatctgatga accagtgatt ggggagacac gtattggcgg acagccagtg
     1681 ccggtagtgc gtttcaaggg gttcccaccg agcaaagatg caagaggtga cattgaatct
     1741 atgaatctcc tagccggaca gggtgtgggt ttggtgaatg aaattaaacc cgctgctaca
     1801 atcgtgcgtg agttggttga aggagcacga gagattatcc aaaagcggct tgacagaatc
     1861 ctagctaata aatgagcgta attttgtatc tgcaatgttt actaacgccg atatttaggg
     1921 tcatactaat aagtaaacat tgctcacctt ggaggagatt gagattacgg aggggaaaag
     1981 acgaactata ttgaggggaa atgcgtcacc gccgttcaat cagattaaag gattatgatt
     2041 attcccagga aggggtttat tttgctacca tttgcattca gaatcggcaa tgtttattag
     2101 gatatgttca agacgggaca atgtttttaa gccatgctgg ggaaatggta aagtcggtat
     2161 ggatggattt gcctaatcgt tttgatcata tccttttggt tgaatttgtt gtaatgccaa
     2221 atcacatcca tgggattatc atcatccatc gtaggggcga atcttgtatt cgccctaatt
     2281 cttcaatggg cgatcaaaag gatcgcccct acggaaccga acccgattca ataggtcgca
     2341 ttattcaagc atttaagtcc atcacgacta acaaacatat ttggggtgtt cgagaaaaga
     2401 aatggccggc gtttcccaac agactatggc agcgcaatta ttacgaacac atcgttcgga
     2461 atgaaaagga attacataac cttcgcgaat atgttgccaa taatccaaca aattgggcag
     2521 aagatgagga aaatcctgat aaataaacat ggtagagacg taaaattttg cgtctctaca
     2581 ttagagggta gccattatgt caaaagaagt ggaaattagg tttagtattg tactagatga
     2641 caacaacata ccggttgcca tcttttggga agcatcggat tcagaattcg aaggaaaaaa
     2701 gccgtgcgat tctgtaatga tatcaatttg ggataagaaa gaaaaaaatg cattgagcat
     2761 tgacctttgg accaaggata tgattgttgg tgaaatgaat gcccattttt ttcttacact
     2821 tatgaaaatg gctgaaacct atgaaagagc aacaaaaaat aatgaagttg caaatatgat
     2881 aaaagggttt gccaatgatt tcgcaaccaa agtagaagaa cttacaaaat aagtaatgaa
     2941 taaaacgaca tcaaaattcg gacataacct ctttcgcaaa aaaacgtaga aattccgggt
     3001 tcaaaatatc atagaaaaat attgtgaatt tgcttacacc tttgttcact ttgtgtgtaa
     3061 ttttctcaat acattgatct ggtgttccaa cgattcctgt tttttcaata tccccaaaaa
     3121 agctaaaccg cttttttgcc tttggaagtt tttcttcaag ttccgtttta tctttcgcta
     3181 tcacacacac agtctgttca gaaatttcta gttcacttat atctctacct aaagatttgc
     3241 agtgtgtttt aaggactttt agctttctat cataatctgc cgcattagag gcagggcaat
     3301 tccagccatc tgcaagctct gctacaactc gaagtaggtg cctttctccc gaacctccga
     3361 tagtgattgg tggatgtggt ttttgaatag gcttgggatt gcaatacgca tctctaatct
     3421 gaaaatgttt tccaataaaa gttgcttttt cttgagtcca caatgcccta attattttca
     3481 cagcttcttt taattgttct atcctagtta ttgtatcagg gaatggatag ccataagctt
     3541 tatattcagg ctcaaaccag cctgcaccga ttgcaaattc aagccttccg ttacttatgt
     3601 gatcaagtgt cgaggccatc ttagctagaa gggaagggtg tctaaaagag ttgcaaagaa
     3661 cgagtgtacc aatctttact tttgaggtga tggttgaaag ggcggacata agagtccagg
     3721 cctcaaaaac atcaaagtca atagagccca ttccgaggat atgatcatag aaccaaatag
     3781 agtgataacc cagctcatca caaagctcag ccgtttccct aatcgtttta aaatcaatct
     3841 tttcttggcg taagataacc ccaaatttga cctttttcat gatttaacac ctgctaagga
     3901 gttttatgag tgacaaaaat cactttaaca gactgataat gttgaatcaa atctaattat
     3961 gttatgagct tatacaggaa tttgacactg gagtatagtt gttataagtt aaaaagctac
     4021 ctaattctca gtgaaagtac tacaaaaaat gacacaggtg ttattacaaa gtgggttcgg
     4081 tttaaaagtg ggctatatta ttattaataa agtccctcct gtcataatgc tgatcatcct
     4141 tttcatgctt atttggggct gtgaacagaa agagccaaaa cagagcgagc aaaagcagaa
     4201 tgaacgaaaa acagttgatc agcaaggtag tgaaaataag aggagccata ctcaaggagg
     4261 aaatcctatg gtgataatgt ctacgtcagt gggagatatt aaaatagaac tgtattcgga
     4321 taaggcccca atgactgtta ataattttct cgcctatgtt aatgacaagt tttacaatgg
     4381 taccatcttt catcgggtca ttcctgggtt tatgattcag ggaggtggat ttacaccgga
     4441 aatgcaacag aaggcgacta aaccacccat aaagaacgag gcgaataatg gtcttaagaa
     4501 tgataccggc accatcgcca tggcgcgcac aagcgtggta gacagtgcta cttctcagtt
     4561 ctttatcaat cttacaggta atgacttctt aaaccatggt gatagggact ttggctatgc
     4621 cgtttttggt aaagtggttg aaggaatgga tgtagtaaat aaaatcgccg gagtgaaaac
     4681 aggcagtcac gggatgcaca gggacgttcc tttagaccct gtagaagtag aatctattcg
     4741 tgtaattgaa ccagtaggga aataaaagtg tttttttagt ttttagagtt taaatctttt
     4801 ctataaatca ccttatcatt atattttgca taaaaatcac taattctaga aacctcgata
     4861 aacccatttc tcatgtaaaa actccttgca ggttcgtact ccggccttga tgatgtctct
     4921 accattacag ctcttgcact cttagctttt aaatcatcct ccataaacgt caggagcttt
     4981 tttgcaattc cctttccttg aaactcatct gatataacaa cccagtaaat atcccagacg
     5041 gcgtctgtta gcgggatttt accatagcat atatagccaa gaaccgtgcc cttctcatct
     5101 gttgcaacct ttatgatgta atctggatta cctgaaaggc aagaatctat taactcaagt
     5161 gctactttaa tttcttcaga agaaaatatc ttaacttcgg caagaataca ttcaatcctt
     5221 cccctatcct gtattcccaa gctccttact attagtgtca taatatctcg ctttcttcct
     5281 ttgaatcgca aaccctacaa cttgattaac aaatgccttg tagctaatac ctgctgcttt
     5341 taaagccttt gaaaagcctg attcgggtga tatatcagga ttagggttta cctcaatgac
     5401 ataaatcttt ccaaggctat ccattctcat atccaccctt gcgtagtctc tgcatcctat
     5461 tgttctagag gcttttaggg ctatgtcttt tattcttttt tccgttttct tattcaaact
     5521 agcaggacaa attgtaattg tattcttgta ttcattac
//
LOCUS       DATDRL010000248        13564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2400290, whole genome shotgun sequence.
ACCESSION   DATDRL010000248 DATDRL010000000
VERSION     DATDRL010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13564
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2400290"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3202
                     /gene="gltB"
                     /locus_tag="VI935_08455"
     CDS             <1..3202
                     /gene="gltB"
                     /locus_tag="VI935_08455"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HLE25665.1"
                     /translation="EEVLQRQQAFGYTFEELRIILAPMAKDGVEPVGSMGNDTPLAVL
                     SDKPQLLYNYFKQLFAQVTNPPLDAIREEIITATETAIGPERNLLLPEPESCHQIKLK
                     TPILTNEELNKLKHINTPSFKSVILPILFKVSEGGQGLEKAIEELYRRASLAISEGAN
                     ILILSDRGVNRENAPIPALLAVSGLHHHLIREGTRTKVGLVLESGEPREVHHFALLIG
                     YGAGGINPYLAYETLDDMIKEGLLTDITYKEAVKNYNKAIVKGVVKVMSKMGISTIQS
                     YRGAQIFEAVGLNHTMIDKYFAWTSSRVEGVGVDVIAKEVQMRHERAFSDRQVDGNTL
                     DVGGKYQWRQEGEHHLFNPETVHKLQYACRTNNYKVFKEYSELVDNQTKRLCTLRGIL
                     ELKFTPNPIPLEEVEPVESIRKRFKTGAMSYGSISKEAHESLAIAMNRIGGKSNTGEG
                     GEDPVRFIPDNNGDSRNSAIKQVASGRFGVTSQYLVNAKELQIKMAQGSKPGEGGQLP
                     GAKVYPWIAKVRYSTPGVGLISPPPHHDIYSIEDLAELIHDLKNSNPEARINVKLVSE
                     VGVGTIAAGVAKGHADVVLISGHDGGTGASPQSSIHSAGLPWELGLAETHQTLVLNNL
                     RSRIVVETDGQLKTGRDVVIAALLGAEEFGFATAPIVVLGCIMMRVCHLDTCPVGVAT
                     QNPELRKKFEGKPEHVVNFMRFIAEEVRDLMSKLGFRTLNEMVGRTDKLEMRKAIDHW
                     KAKGLDFSKILYQPDASPDVGRYCQIPQDHGLDQALDNRVLLNLCEPALERSEPVEAI
                     LPIRNTNRVVGTILGSEITRRYGAEGLPEDTIRLHFQGSAGQSFGAFVPCGITLILEG
                     DSNDYIGKGLSGGKIIVYPPRGSTFVPEENIIIGNVAFYGATGGEVYIRGMSGERFCV
                     RNSGVHAVVEAVGDHCCEYMTGGRVVVLGPTGRNFAAGMSGGIAYVLDEPGDFNRRCN
                     QDMIYLERLEDENEINELEAMIKKHAEYTKSQLAWKILRRWKEMVPKFIKVMPKDYKR
                     MLNAVKRVGEAGLSGDEAVMAAFEENKRDLARVSGN"
     gene            3887..5371
                     /locus_tag="VI935_08460"
     CDS             3887..5371
                     /locus_tag="VI935_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HLE25666.1"
                     /translation="MGKPTGFMEYDRELPEERPPAERIHDWLEFHLHLPEKKLRTQGA
                     RCMDCGVPFCHTGIILGGMTSGCPINNLIPEWNDLIYRGLWREALDRLHKTNNFPEFT
                     GRICPAPCEAACVLGINEPPVTIKNIECAIIDKGFEEGWVVPEPPTKRTGKKVAIVGS
                     GPAGLACAAQLNKVGHRVTVFERADRIGGLLMYGIPNPHLDKKIVERRLDLLAKEGIK
                     FVTNTEVGKDYPSDRLFKEFDAVVLCGGASKPRDLPIEGRELKGIHFAMEFLEANTKS
                     LLDSDLQDGNYISAKAKDVIILGGGDTGTDCVATAIRHGCKSLLQFEILPMPPTERAP
                     DNPWPEWPKVYFLDYGQKEAAAVFGSDPRRYSVMTKRFVGDKKGNVKELHTVRIEWQK
                     GDNGRPVFKEIPDTEKVLPVQLVLLALGFLGPEETLLSQLGVERDERSNAKAEYGRFA
                     TNIKGVFAAGDMRRGQSLVVWAINEGRGAARECDRYLMGSTNLP"
     gene            complement(5783..7573)
                     /locus_tag="VI935_08465"
     CDS             complement(5783..7573)
                     /locus_tag="VI935_08465"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HLE25667.1"
                     /translation="MQPLLDTVFTSIILTGIMDTVRIGLGQIDVTVGDLDGNLNKILR
                     YIESAKKLGVDILCFPELAITGYPPEDLLLKPSFIEDNLEALAKVSNATEGLTLIVGF
                     VDRKEDIYNAAAIIHNKRLVDVYHKRYLPNYGVFDENRYFQSGFRSPVYKLGRVIFGV
                     NVCEDIWYPGDPTREQALLGDAQIIINISSSPYFVSKVKSRERMLSVRASDYSVIVVF
                     CNLIGGQDELVFDGHSVVIGEQGDIISRASGFKEELLVADVSPGRVFRSSLHDPRKRK
                     EKQALLHESKQAEIIEIAEEEPLRNSRPRIAPKVEEILRPEEEVFCALVLGTRDYVTK
                     NGFKKVVMGLSGGIDSSLVAVIAVEALGKENVIGVSMPSRYTSSGSIADAEKISENLG
                     IELITVPIEKSFSAYLDLFTQAFSGKKQDVTEENLQARIRGNILMALSNKFGWLVLTT
                     GNKSETSVGYCTLYGDMAGGFAVIKDVPKTFVYRIAEFYNNWKGKDIIPKTVIGKPPS
                     AELRPNQKDTDSLPAYEILDPILKAYVEDDLSIEEIIALGFDENVVKRVIKLVDSNEY
                     KRRQAAPGIKITVRAFGKDRRFPITNLYRG"
     gene            complement(7592..8242)
                     /locus_tag="VI935_08470"
     CDS             complement(7592..8242)
                     /locus_tag="VI935_08470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HLE25668.1"
                     /translation="MKNKLRFQSLGSIGDYVAVAYKLTKDTIKVTDSSIVAYVIVIFI
                     ITLLRRFLPSFVLPIAAFLFFSAPYLMKSKVTGLKWNLRGVLLGVAVSVIILSIYLVI
                     VNKPIKLSKISFTLLILQLFLVSLPEEVFFRGYLQEKIGNNLKGILIVSSLFAIGHFA
                     TACLGGGYVGMVCLKTLLTFFPSIVMGFMYAKTGTIWGNVVFHFLANVVYEGTGGL"
     gene            complement(8336..8962)
                     /locus_tag="VI935_08475"
     CDS             complement(8336..8962)
                     /locus_tag="VI935_08475"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HLE25669.1"
                     /translation="MVSVFRKSDILSSVILGEIVAVFLILISKNLERDVPILDILVRS
                     KWLIFLLVPALATLGVYGTFYVGRRHPVFFQFGKFITIGFSNAAIDFGILNLLIFITG
                     IERGYFYSVFKAISFVFAVTNSYLWNRFWTFESHETNETGKQFFQFVAISGIGFGINV
                     IIASFIVNVVGPVGEISPRLWANVGASAAIVISVFWNFLGYKFIVFKK"
     gene            complement(9397..10704)
                     /locus_tag="VI935_08480"
     CDS             complement(9397..10704)
                     /locus_tag="VI935_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HLE25670.1"
                     /translation="MLLKGFVRKNKIEVLLFFLLIISLGLKLYRLSIPKSYYFDEIYY
                     AFTAEEMAKGNRAGWEVGHTAPKGFAYEWSHPHLGKELSAIGILIFGDNTFGWRFFQA
                     IFGGFGTLFIYLLGKELFQSKRAGLIAAFLFTFESFIYVLSRITMVDIFLMSFILLAS
                     LFFVKYARTQKNAFLLLTGFFCGTAISVKWSGIYALEFLATISFLFIFYREVYLGVQK
                     RSSLYYTFLEIIPKIFLVFVIIPGVVYLATYIPFFLYGNSFSDFISLQSSMYGYHKGL
                     TATHPYQSSWWKWPLLLRSVYLYLGSGEKESAHIYAIGNPFIWWSGCVFLALSVVQVI
                     RKELPALAFVTLSVFAYWLPWALSPRKLVFVYHFLPSLPFVLLIIAYFLDLLWTKSRY
                     GSAFVIAYLTISVGMFIYFYPILAAIPVSNASVESYFWLKSWR"
     gene            complement(10937..12406)
                     /locus_tag="VI935_08485"
     CDS             complement(10937..12406)
                     /locus_tag="VI935_08485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLE25671.1"
                     /translation="MNQEEGFAGMGALDVIEGRLSNPFGFGPVTAWGWTYYSALYFYG
                     QALFIKLFDVNVFSIRLFSAISGTLSVIFAYLLAREIWGRSVGIISCILVAVAGIHIH
                     FSRFGFPFIHTCLFGTMTLFFIVRSIKNKAPADFALAGFSIGLAQYFWTASRILPFIA
                     FIFFIFKIIQKRNFLPQYYRGLIILFAAFVLAVAPLALPIEKKFWNFTEGASRDFVFG
                     GWMATDYMNRLQSEESLWYILGEQIVRSFLSFNFYPDKGYLYGGVYGGLNGPMLEFFT
                     SIFFVFGLFYIVFAWEKSAHLLLLIAFFGSVFGLVALTTHPPNYQRMVVILSLPYIFA
                     AVGIWKCFEWLRYIITYRRPLYVTLCCLLLFLGWRNYELYFSEYLNSRYWGYTDPAIS
                     IAHFVKSLDLTYRIYLPAPYMHVPWTVKFITEDAPYDMDYRPLLQVLQDLETNVGKTV
                     FILLPEQANNLDLLQKRFPGGITRSLSDDFQSIKIYKIE"
     gene            complement(13099..>13564)
                     /locus_tag="VI935_08490"
     CDS             complement(13099..>13564)
                     /locus_tag="VI935_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLE25672.1"
                     /translation="NVDFQVNTINKMELPEGYYSKVVCQEVIEHLFEDEINQLMSKSY
                     KTLKPGGRFLITTPNYSSLWPVIEYIMDKLKLSPPMAGHQHVTKFNTSKLKNWLESFQ
                     FQIIDAGGFNTFAPWVGFLPASLVRGINRFELNHISVLGNLIYVIAQKPFWN"
BASE COUNT         4248 a         2972 c         2787 g         3557 t
ORIGIN      
        1 cgaagaagtt cttcagcgcc aacaggcgtt tggctataca tttgaggagc ttcgcattat
       61 tttggctcca atggcaaagg acggcgttga accagtcggg tctatgggta atgacacacc
      121 gcttgcagtg ctttcagata agccccagtt gctctacaac tattttaaac agctatttgc
      181 acaggttact aatcccccgc tagatgcaat acgtgaggag atcataactg caacagaaac
      241 agccattggc cccgaacgca atctacttct acccgagccc gaaagctgcc accaaatcaa
      301 gcttaaaacg ccaattctta caaatgagga actaaacaag ctcaaacata taaatacccc
      361 cagcttcaag tcagtgatct tgccaatcct cttcaaggtc tcagagggag gtcaaggact
      421 agagaaggca atagaagagc tttatagaag agctagcctc gctatatcag agggggcgaa
      481 catcttgatt ctatccgacc gtggtgtaaa tcgtgaaaac gcaccaatac cagcccttct
      541 tgccgtatcg ggtctccatc atcaccttat tcgtgaaggc acacgcacta aagtaggatt
      601 ggtactagag tcaggagaac cacgagaggt acaccatttt gcccttttga tcgggtatgg
      661 cgctggaggg ataaatccct accttgcgta tgaaaccctt gacgatatga tcaaggaggg
      721 cttgcttacg gatataactt ataaagaggc tgtgaaaaat tataataaag ccatcgtaaa
      781 gggagtcgtc aaggtgatgt cgaagatggg gatatccact atccaaagtt accgcggcgc
      841 ccagatcttt gaggctgtcg gccttaacca cacaatgatt gataagtact ttgcctggac
      901 ctcttcacgc gtagaaggcg tgggggttga cgttatcgcg aaagaggtac aaatgaggca
      961 tgagcgcgcc ttctccgacc gtcaggtaga cggcaataca cttgatgtcg gtggcaagta
     1021 tcaatggaga caagagggag aacatcatct cttcaatccg gagactgtac acaagcttca
     1081 gtacgcctgt cgcacgaata actataaggt cttcaaagag tattccgaac tcgtagacaa
     1141 tcagactaaa aggctctgta ccttacgagg gattttagaa ctaaagttta cgcctaatcc
     1201 tattccactt gaggaagtag agccggttga atccatacgc aagcgcttca aaacaggggc
     1261 gatgtcttat ggctccataa gcaaggaggc acacgaaagc cttgcaatag ccatgaacag
     1321 gattggcggt aagagcaaca caggtgaagg tggtgaggat cccgtacgct ttatacccga
     1381 taataacggt gattcacgca atagtgccat caagcaagtt gcttcaggaa ggttcggcgt
     1441 tactagccag tatctggtca atgccaagga gctacaaatc aagatggctc aaggctcaaa
     1501 acctggcgaa ggagggcagt tgccaggagc caaggtctat ccatggattg caaaagtaag
     1561 atactctacc ccgggtgttg gtctgatttc acccccaccg catcatgata tttactcgat
     1621 cgaagatctt gccgagttga tccacgatct gaagaattcg aatccagaag ctaggattaa
     1681 tgtcaagcta gtctccgaag ttggtgttgg cactatcgct gccggtgtag caaagggtca
     1741 cgctgatgta gtgctcataa gcggtcatga tggaggcaca ggcgcctcac cccagtccag
     1801 tattcatagt gccggtctac catgggagtt gggtctagcc gaaacccacc agacccttgt
     1861 tcttaacaat cttcgcagtc gaattgttgt agaaaccgac ggacaactca agactggtcg
     1921 ggatgttgtc atagctgctc ttcttggcgc agaagagttc ggttttgcaa ccgcaccaat
     1981 tgtcgtacta ggctgtatta tgatgcgcgt ctgtcaccta gacacctgtc ctgttggagt
     2041 ggcaacccag aacccggagc tccgtaagaa gtttgaggga aagcccgaac acgtggtcaa
     2101 cttcatgcga tttatagccg aagaggtgcg cgatcttatg tcaaagctcg gcttccgtac
     2161 attaaatgag atggtaggac gaaccgacaa gcttgaaatg agaaaggcaa ttgaccattg
     2221 gaaggcgaaa gggcttgatt tctcgaagat actttatcag cctgatgcct ctcctgatgt
     2281 tggtcggtat tgccagattc cccaggacca tggcctggat caagcccttg ataaccgtgt
     2341 gcttcttaac ctatgtgagc cagcacttga gcgcagtgag ccggttgaag caatactccc
     2401 cattcgtaac actaaccgcg ttgtgggaac cattctcggt agcgaaatta ccaggcgata
     2461 cggagccgaa ggcctccccg aggacacaat tagacttcac ttccaaggat cggcaggaca
     2521 aagcttcggt gcatttgttc cgtgtggcat aacgctcata ctcgagggtg attcaaacga
     2581 ttatataggc aaggggcttt ccggtggtaa gatcatagtc tacccaccta gggggtcaac
     2641 attcgttcca gaggaaaaca tcatcattgg taacgtagcg ttctacggtg ctaccggagg
     2701 ggaagtctac atccgcggca tgtctggaga aagattctgt gtgcgtaaca gcggggttca
     2761 cgcagttgtt gaggcagttg gagaccattg ctgtgaatac atgaccggtg gacgcgttgt
     2821 agtgcttggt cctacaggaa ggaatttcgc ggcaggcatg tcaggcggta ttgcatatgt
     2881 tcttgatgag ccaggtgatt ttaaccgccg atgtaaccaa gacatgatct accttgagag
     2941 gttagaagac gagaatgaga ttaatgaatt agaagcaatg attaagaaac atgctgagta
     3001 cacgaagagt caactggcat ggaagatcct aagaagatgg aaagaaatgg ttcctaagtt
     3061 tattaaggtc atgcccaagg actataagcg tatgctcaat gctgtaaaac gtgtcggaga
     3121 ggcaggctta agcggtgatg aagctgtcat ggctgctttc gaagagaata agcgcgatct
     3181 ggcacgcgtt agcggaaact aagttgccat tcgtgattcg tgattagtgg ttcgagacac
     3241 gattcacgaa tcacaaagcc cgactagtat tttccaaacg catacagaac taattaccca
     3301 actttctatt tcacaagctt aacagtgaag gctaaaaata aaccgagtat cagtcttcac
     3361 ccattcacaa aaaaaagaag accgcagtag aaattaattt cgacattctt acgaaaatta
     3421 tcgtcaatga aatatcattg agtaagcttt aaggtagaag catattatta gtagacattc
     3481 cttttgtctg cgaagagtaa agcgatcaag caatcgagaa acgagattgc ttcgctaccg
     3541 caatgacaac tggaagaaat agcttcacgg agtctacgct gagtgaaaat aatgtgttcg
     3601 caacgacaag ataaaaaagt gtcattgcga gaagtgaaac gacgaaggaa tccaataaag
     3661 agttatggat tcgctagttg atgacagtgt acaccaccct gagcgaatca catggaatcg
     3721 ttgagttgaa ctaaaacttt gaatttaatt ttattggtgt agattattga acttgccctg
     3781 cacaatgttc atatgttaca aatactgtag tagctaataa agttgactta aacatctctt
     3841 cgtgttctta ttaattaaat aggtataata aatcaaggta aattcaatgg gtaagccaac
     3901 cggatttatg gaatatgacc gtgagctccc ggaagaacgt ccaccagccg aaaggatcca
     3961 cgattggcta gagtttcatt tgcacctccc tgagaaaaag ctacgtactc agggtgctcg
     4021 ctgtatggac tgtggtgtac ccttctgcca caccggaatc atacttggcg gtatgacctc
     4081 cggctgccca atcaacaatc ttattccgga atggaacgac ctgatctacc gcggtctctg
     4141 gagagaagcg ctcgatcgtt tgcacaagac taacaatttc cctgagttca caggcaggat
     4201 ctgccctgca ccatgtgagg cagcatgtgt tctcggaatt aacgagcctc cggttacgat
     4261 taaaaacatt gaatgcgcca ttattgacaa gggctttgaa gagggatggg tagttccaga
     4321 gcctcccaca aaacgaacgg gaaagaaggt agctattgtg ggttctggac cagccgggct
     4381 tgcatgcgca gcacagctta ataaagtggg tcaccgggtt actgtttttg agcgcgctga
     4441 tagaattggt ggattactta tgtacggcat tcctaatcca catctcgata agaaaatagt
     4501 agagcgtcgc cttgacctat tggctaaaga gggcatcaag ttcgtaacaa ataccgaggt
     4561 cggaaaggac tacccttctg acaggctttt taaggaattc gacgctgtag ttctatgcgg
     4621 tggagcttct aaaccgcgtg acttgcctat tgagggccgg gagcttaagg gtatccattt
     4681 cgctatggag tttctcgagg ccaatacaaa gagccttctt gacagcgatc ttcaagatgg
     4741 aaactacatc tcagctaagg ccaaggatgt gattatcctt ggcggcggcg atacaggtac
     4801 agattgcgtt gctaccgcaa ttcgccacgg atgtaagagc ttattacagt ttgagatcct
     4861 tccaatgccg cccactgagc gcgcccctga caacccctgg cctgagtggc ctaaagtcta
     4921 tttccttgac tacggccaga aggaagcagc cgccgtgttc ggaagcgatc ctcgtaggta
     4981 tagcgtcatg accaaaaggt ttgttggaga taaaaagggc aatgtcaaag agttacacac
     5041 tgtgcggatc gaatggcaaa aaggcgacaa cgggcgtcct gtcttcaagg aaatccccga
     5101 cactgagaag gtcttgcctg tgcaacttgt gctgctcgcc ctagggttcc ttggtcctga
     5161 agaaactcta ctttctcaac tcggcgttga gcgtgatgaa cgttccaatg ctaaggcaga
     5221 gtatggaagg ttcgcgacaa acataaaagg cgtttttgca gccggtgata tgcgccgcgg
     5281 tcaaagcctc gtagtatggg ctatcaatga gggacgcggt gccgcacgtg agtgcgaccg
     5341 ctacttaatg ggatcaacta atctaccctg atagtaaaaa agcgttactc tcttactata
     5401 ccattccaaa ttattaattg gttaatcatg gtttcctttg atacagacgg gacatttcct
     5461 tcgttatgcc gcttctactc gtggcgactc ctaacctgtc atacccgagt acagttaccc
     5521 ccgattaaat cgggaaagga atgcagctct tacccaaggg gcttctaacc tgtcataccc
     5581 gagtgcagtt atcgggtatc catatttttt ttggattccc aataaagctt gtacccacat
     5641 gcctttagtg ggtaacgttt gggaatgact aaggcataat ggattcctgc ttaaacccgt
     5701 aacagaatgc tcttatcaca taacatgcaa atgacattct gttggcactt taaacacttt
     5761 ccattgaaaa tggtatacta tattaccctc tatacaaatt tgtgattgga aagcgacggt
     5821 ccttgccaaa ggctcttaca gtaatcttta tgcctggggc agcctgccta cgcttgtact
     5881 cgtttgaatc tactaatttt atcaccctct tcaccacatt ttcatcaaac cctaaagcaa
     5941 taatctcctc tatgcttaaa tcgtcctcta catatgcctt gagtattgga tcgagaatct
     6001 cgtaagccgg aagtgaatca gtatccttct gattaggtct tagctccgct gacggcggtt
     6061 tccctatgac ggttttggga ataatgtctt ttcccttcca attgttataa aattccgcta
     6121 tgcgataaac aaatgtcttc ggtacgtctt ttatcacggc aaaccctcca gccatgtccc
     6181 cgtataatgt gcagtatcca acgctagtct cgctcttgtt accggttgta agcacaagcc
     6241 atccaaattt attagaaagc gccatgagta tattaccacg aatccttgcc tgaaggtttt
     6301 cctcagtcac gtcttgcttt ttcccactaa aggcttgagt aaacaaatca aggtaagcgc
     6361 tgaatgattt ctctatggga acggtgataa gctcaatccc aaggttctcg gagatttttt
     6421 cggcatccgc tatacttcct gatgaggtat acctagaagg catcgaaacc ccgataacgt
     6481 tttcttttcc aagtgcctca acagcaatta cagcgacaag tgatgagtca ataccaccgc
     6541 ttaggcccat tacaaccttc ttaaaaccat tcttcgtgac atagtctcta gttccaagta
     6601 ctaaagcaca aaacacctct tcttctggac gcagaatttc ctctacctta ggggcaatac
     6661 gggggcgaga attccttaaa ggctcttcct cggcaatttc aataatctct gcctgttttg
     6721 attcatgcaa aagagcctgc ttctcctttc tttttctggg atcgtgaagg gatgagcgaa
     6781 aaaccctacc tggactgacg tccgcaacaa gaagctcctc tttaaaccct gaagcacggg
     6841 atattatatc gccttgttca cctattacaa cgctatgtcc atcaaataca agctcatcct
     6901 gaccgcctat caggttacaa aagacgacta tcactgagta atcagatgcc cttacggaga
     6961 gcatcctttc cctcgatttg acctttgaga caaaataagg ggaggaagag atatttatta
     7021 ttatctgggc atcacctaat agagcctgct ccctagtcgg gtctcccgga taccagatgt
     7081 cttcacaaac atttacccca aagatgaccc taccaagttt gtaaacaggg gagcgaaatc
     7141 cggattgaaa atacctattt tcatcaaaaa caccatagtt cggaagataa cgcttgtggt
     7201 aaacgtcaac taacctctta ttatggatta tagcggcagc gttatagata tcctcctttc
     7261 tatctacaaa accaacaatt aatgtaagcc cttctgtagc attagaaacc ttagctaaag
     7321 cttctaagtt atcttctata aagctcggtt tcagcagtaa atcctcagga ggatagccag
     7381 tgattgcaag ctcaggaaaa cataagatgt caacaccgag tttttttgct gattctatgt
     7441 atcgaagaat cttatttaga ttcccatcta aatcaccaac ggttacatca atctgtccca
     7501 accctatcct aacagtatcc atgatgcctg ttagtataat acttgtaaaa accgtatcaa
     7561 gtaggggctg catatatgcg gcccctaccc attacaatcc accagtaccc tcataaacaa
     7621 cgttagcaag aaaatggaat acaacgtttc cccagattgt tcccgtttta gcatacataa
     7681 aacccataac gatagagggg aaaaaagtaa gaagtgtttt taaacagacc atcccaacgt
     7741 aacccccacc caaacacgct gtagcaaaat gcccaatagc aaacagtgag ctgactatta
     7801 atatgccttt taaattattc ccaattttct cctgaaggta gcctcgaaaa aaaacctcct
     7861 caggtaaaga gactagaaaa agttgcaaga taagaagtgt aaaagaaatt ttactaagct
     7921 ttataggttt atttacaata actagataaa tagaaagaat aattaccgat acagcaactc
     7981 ctaatagcac gccacggaga ttccacttaa ggccagttac cttacttttc atcaaataag
     8041 gtgcagagaa aaacaaaaaa gccgcaattg gtagtacaaa actgggaaga aatcttctaa
     8101 gcagtgtgat gataaatata acaatcacat aggctacaat agaactatcg gtgaccttga
     8161 tagtatcttt tgtaagttta tatgcgacag ctacgtagtc tcctatagaa cctaaggact
     8221 gaaaccgcag tttatttttc ataaatgaaa ttccgaagtg aaaagcttta ccaaaattta
     8281 aataagattt taaaacacat aagataccag gattagattt ttaaatttca gatttttact
     8341 ttttaaaaac tataaattta tatcccaaaa aattccaaaa gaccgaaatc actatagctg
     8401 cggaagcccc aacgtttgcc cagagccttg gagagatttc accgaccggt ccgacaacgt
     8461 ttacaataaa agaggcgata attacattga ttccaaatcc gataccactt attgctacaa
     8521 actgaaagaa ctgctttccc gtttcgttgg tctcatggct ctcaaacgtc cagaatctat
     8581 tccacaaata actgtttgtt accgcaaata cgaatgagat ggctttgaaa actgagtaaa
     8641 aatatcctct ttcaatccct gtaatgaata ttaaaaggtt cagtatccca aaatcaatag
     8701 cagcatttga aaaccctatc gtaatgaact taccaaactg aaaaaaaact ggatgtcttc
     8761 ttccaacata gaatgttcca taaaccccaa gtgttgctag agcgggaact aaaaggaaaa
     8821 ttaaccactt agaccttaca agaatatcta atatcggaac atctctctca aggtttttcg
     8881 agattagaat tagaaaaacc gcaacaatct caccaagtat aacactgctt aggatgtctg
     8941 attttctaaa tacgctcacc atttttatgc tataactgta tcataataat accgattttc
     9001 tatccaaatc tggcgttaac agtcttgtca ctgctagcga ttataagaag tgaagaattc
     9061 taaacagttt tcagccatca gcattcagct ataaagtttt tcctgacgac tgattactga
     9121 tagctggtgg ttgattaaat tgtggattgc ttcaggacgt agactctacc tagcgtatgt
     9181 gaagtgaccc tcgcaaaaac atttccgttc attcacaatc aatcttttgt agatagagat
     9241 tgcttcgtcg tcgcctttta tttccatttc tcgaacactg ctctcgaaat aatgctcctc
     9301 gcaatgaccg ttcgtcattt ataccccatt caacaaggaa atggtattag gatcagagat
     9361 cacgtttaat aaaacttcgt caggggcagt taataactat ctccacgact tcagccaaaa
     9421 gtaactttca actgaggcat ttgatactgg aatcgccgct agtatagggt aaaagtaaat
     9481 aaacatacca acggaaatag ttagatacgc gatgacaaag gcacttccat aacgggattt
     9541 tgtccatagt aaatcaagaa agtacgcaat tattagaagt acaaaaggga gggagggtag
     9601 aaaatggtag acaaacacaa gctttcttgg agatagtgcc catggaagcc agtaagcaaa
     9661 aacgcttagt gttacaaaag caagtgcagg tagctccttt cgtattactt ggacaacgct
     9721 taaggcgaga aacacgcatc ctgaccacca aataaacgga tttcctatcg cataaatatg
     9781 ggcgctttct ttttcaccac ttcctaaata taaataaaca gatctaagaa gtagtggcca
     9841 tttccaccaa gaagactgat agggatgtgt tgcagtaagc cctttatggt aaccatacat
     9901 gctactttga agagatatga aatcgctaaa ggaatttcca taaagaaaaa aaggaatata
     9961 tgttgcaaga taaaccacac ctggaataat tacaaatacg aggaaaatct taggtatgat
    10021 ttctaaaaat gtataataca aagaacttct tttttgaaca cctaaataaa cctcgcgata
    10081 gaatataaac aaaaacgaaa tagtcgctaa aaactcaaga gcataaatac cgctccattt
    10141 tactgatata gcagtcccac aaaaaaaacc agtaagcaaa agaaaggcat ttttttgagt
    10201 ccttgcatac ttcacaaaaa aaagcgatgc gagtagaata aagctcataa gaaaaatatc
    10261 caccatagtg atgcgactta gaacatatat aaatgattca aacgtaaata aaaaagccgc
    10321 aataagccct gctcttttac tttgaaaaag ctctttacca agtaggtaaa taaacaaagt
    10381 accaaatccc ccgaaaatag cctggaagaa acgccatcca aaagtgttat cgccaaaaat
    10441 caagatgcca attgcgctta gttcctttcc aagatgggga tgcgaccatt cataagcaaa
    10501 cccctttggt gctgtgtgtc caacctccca ccccgctcga tttcctttcg ccatctcctc
    10561 tgctgtaaaa gcatagtaga tctcatcaaa atagtagctc tttggaatac tgagacggta
    10621 taacttgagg cctaggctta taatgagtaa aaagaaaaga agaacttcaa tcttgttttt
    10681 tcttacaaac cctttaagca acatgtttta caatgttcta tagaaatcgg tcactaacat
    10741 cccttatctt atcatattgg ttctgtgatg aagtatgcta aaaaaagatt ataatgtact
    10801 cgtctgttta atccaagagc aagccttagt tgtgtttttt atctattttt ttgcttttct
    10861 tcatcctgaa acgcttcagc gtgttatacg aaagcgctta aataacttat tatatttttt
    10921 gtcctcttgg attaccttac tcaatcttat aaatcttgat agattggaaa tcatcactca
    10981 aacttcgagt aatgccacct gggaatcttt tttgcagcaa atctaaattg ttggcttgtt
    11041 ccggaagaag tataaacacc gtcttaccaa cattcgtttc taaatcctgc aagacttgga
    11101 gtaatggacg atagtccata tcatatgggg catcttctgt aatgaatttc accgtccacg
    11161 ggacatgcat atagggagcc ggcagatata tcctataggt gagatcaagg gatttaacaa
    11221 aatgtgctat tgagatggct ggatcggtat agccccaata tctgctattc aagtactcag
    11281 aaaaatacag ctcgtaattt ctccacccta aaaataacag caaacaacaa agtgtcacat
    11341 atagaggtct acggtaagta ataatatacc gtaaccattc aaagcatttc cagattccaa
    11401 cggctgcaaa aatatatgga agagacaaaa tgaccaccat gcgttgataa ttaggaggat
    11461 gtgtcgttaa tgccacaaga ccgaagacac ttccaaagaa ggctataagc aataacaggt
    11521 gagcagattt ttcccaagca aacacgatat agaacaatcc aaagacgaag aagatagacg
    11581 taaaaaactc cagcatgggc ccatttagac ctccataaac acctccataa agataacctt
    11641 tgtccggata aaaattaaaa gagagaaaag agcgaactat ctgttccccc aagatgtacc
    11701 agagactttc ctcagattgc agtctattca tataatcagt cgccatccat cctccgaaga
    11761 caaaatctcg ggaagcgcct tctgtaaagt tccagaattt tttctcaatg ggtagcgcta
    11821 aaggtgcaac ggctaagaca aaggctgcaa agaggataat taagcctcta taatactgag
    11881 gcaagaagtt tcttttttga atgatcttaa agataaagaa aataaaggca atgaaaggga
    11941 ggatacggga tgcagtccaa aaatactgtg ccaatccaat gctaaaacca gctagggcaa
    12001 agtctgccgg agccttattc ttgattgacc tgacaataaa aaataaggtc attgttccga
    12061 agagacaggt atgaataaaa ggaaacccaa atctgctaaa gtggatatgg attccagcca
    12121 ctgctaccag aatgcagctt attatcccaa cactccttcc ccatatctcc cttgccaaca
    12181 ggtaagcgaa gatgacagat aaggtgccgg aaattgcgga gaaaagccta atgctgaaaa
    12241 cattaacgtc aaaaagttta atgaataatg cctggccgta gaaataaagg gctgagtaat
    12301 atgtccaacc ccaagctgtg accggtccaa aaccaaaagg atttgaaagc cttccttcta
    12361 ttacatcgag tgctcccatt cctgcaaacc cctcttcttg attcatcgcc aagggcacga
    12421 cccccaaccg ggaaactctc agccataatg caactaccat tattaatcct actgctataa
    12481 gttcataatg atttttggtt ataaagattt gtaagaactt gattgtaaac ttttttaaat
    12541 ccataaaaga tagaagaaaa agaacaaggc ttgctatcca tgttaacaaa gtaaaaacgg
    12601 aggctgtctc ctgataagta tggaaaagga cataagcaaa caaaacgaat gagaataaca
    12661 gcaaccagaa tcgcctgctt ttaagatcta aacttataac tattccaggt tctattagac
    12721 tcttatcttt atcgacaata tccagaccga aactctccac cttctggtgg aaccgataac
    12781 ttatataaaa aagtataata gctaaaagat ataaggcaga tcccgtttct aagtcccttg
    12841 aatacgaaaa ctggtattga ccagccaacg caaacactat cgacaacaaa aaggaacccg
    12901 taaaaataat tcttcttctc gttttaatct ccagcctaga taagatggcc gaaaaacatc
    12961 ctccgcccaa acttgctttc aaaaaagtct tttataaaca caactaagaa ccttacagct
    13021 agctacagtt tatcggcata tgaacattcc ttcggtcatt gcgaggctgc gaggctcatg
    13081 aaatcagccg aagcaatctc aattccaaaa aggtttttga gcgatgacgt agattaaatt
    13141 gcctaaaaca gaaatatgat tgagttcaaa acggtttatt cctctaacaa gactcgcagg
    13201 caagaagcca acccatgggg caaatgtatt aaatccgccg gcatcaatga tttgaaattg
    13261 aaacgactcg agccagtttt ttaatttact tgtattgaat tttgtgacgt gctggtggcc
    13321 agccataggt gggctcagtt ttaacttatc cattatatac tcgatgacag gccagaggct
    13381 actgtaatta ggtgtggtga tgaggaaccg ccctcctggt ttcaatgttt tataggactt
    13441 agacataagc tggttgattt cgtcttcaaa aagatgctcg attacttctt gacagaccac
    13501 tttactgtaa tagccctccg gtagctccat cttgttgata gtgttcactt gaaagtcaac
    13561 atta
//
LOCUS       DATDRL010000249         3718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_846653, whole genome shotgun sequence.
ACCESSION   DATDRL010000249 DATDRL010000000
VERSION     DATDRL010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846653"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..3186
                     /gene="ileS"
                     /locus_tag="VI935_08495"
     CDS             337..3186
                     /gene="ileS"
                     /locus_tag="VI935_08495"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HLE25673.1"
                     /translation="MAEDKTLDYKNTLNLPRSEFPMKANLNQKEPELLERWGSIKLYE
                     KIREKSQGKPKFILHDGPPYANGHIHLGHCLNKILKDVIVKSKTMFGLDSYYVPGWDC
                     HGLPIEYQVTKDLGAKKETISKAEIRKLCRNYAQKFVGIQRDEFKRIGVFGDWEHPYL
                     TMSYDYEATIARELGRFITVGNLYRGKKPVYWCYSCQTALAEAEVEYEDKTSPSIYVK
                     FPLISEELFAKFFALFEKPVVSKTELKVFALIWTTTPWTLPANLALAFHPDFSYVAAE
                     ILRSSSGQANGEVYIVAEALLETVLGKDAKVIQKFRGKDIDGLKFKHPFYERESIVTL
                     GDFVTLDTGTGIVHIAPGHGQEDYEIGIKYDLDIYAPVDERGRFTKSVEFFGGMFVYD
                     ANKKIIEKLKEVGNLLREEKITHSYPHCWRCKSPIIFRATPQWFISMEKNNLKSKALR
                     EITERVRWIPEWGRERIYNMIENRPDWCVSRQRAWGVPIVVFYCDSCDHTLMNPDIVN
                     YVAQIFEKHGADAWFTHQTNELLPSSTKCPKCGHENFRKETDILDVWFDSGVSYASVL
                     EGNTKLSMPADMYLEGSDQHRGWFHSSLLACVGTRGIAPYKSVLTHGFVVDKEGEKMS
                     KSKGNVIVPSDIIKKYGAEVLRLWVVAEDYRSDIRISTEILERLTESYRKIRNTFRYL
                     LGNLYDFDPVRDALGYEKLEEIDKWILHRLGILSSKILKGYEDYEFHIVYYELQRFCI
                     VDLSAIFLDIQKDVLYTFPPNSEKRRSAQTAIYTILNCLTKLSAPVLSFTADEVWKYM
                     PGEKTESAHLTNFPEPEFINDELEEKWNRILPIRDEILKALERARKDKFIGSSLEAGI
                     SIYVKGELKSFIQENLELLKTTAIVSHIGVAKDSSKGNNVFESVDIPGLWIEVQKAPG
                     EKCERCWTYRTTVGTNPDYPTLCDRCIGHLMVMGT"
     gene            3224..>3718
                     /locus_tag="VI935_08500"
     CDS             3224..>3718
                     /locus_tag="VI935_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25674.1"
                     /translation="MARHKLELLEEGNPEYYRLINESNEIMDEYSRIMGLVHSFKLPT
                     TKIWKFELPKPSLFYRAKADLSIIGKEGMSLANRFLEWNNRAITFCLSPHYRFSPDQE
                     VDKQTSVIHFTNLLIHRADRLNNDITLLQSSYNLRFSEIENTINYWIAISAWGFTFLG
                     FVVSA"
BASE COUNT         1181 a          617 c          828 g         1092 t
ORIGIN      
        1 ttgtaaccag atttaagtat cgctaaactt gcttttgccg ctttatttgc taatcttttt
       61 gtcacaattt aattatttta gttaaagatg gataattagc agtaaaggat taaattaaaa
      121 ctcctcgtga gcaaagttaa aagatgtgga cgtaggtaag tcacatcata aagaagtcat
      181 aatgaattcg gctactttaa tctattttaa ctttatactg tagcagaaat ccctaaattt
      241 caacatggat caatagtttt aatttattcc tatccggtgc caaataactt ttaataaaat
      301 aatggtagga aattatagag gggatattaa cgccacatgg ctgaagataa aactttagat
      361 tataaaaata ctctcaatct tcctcgttca gagttcccga tgaaggctaa tttaaatcaa
      421 aaggaacctg aacttcttga gagatgggga tctataaagc tttatgaaaa gatccgggag
      481 aagtctcaag ggaaaccaaa atttatcctt cacgatggtc caccctatgc aaacggtcat
      541 attcatcttg ggcattgtct taataaaatc cttaaagatg tcatagtaaa atccaagacg
      601 atgtttgggt tggattcata ttatgttcct ggctgggatt gccacgggct tccaatcgaa
      661 tatcaagtga caaaggattt aggtgcgaaa aaagagacga tttcaaaggc tgaaataaga
      721 aagctgtgta gaaactacgc tcagaagttt gttggaattc agcgtgatga gttcaaacgc
      781 attggtgttt ttggagattg ggaacatcct tacctgacaa tgagctatga ttatgaggcc
      841 acaatagcaa gggagttggg aagatttata acggttggaa acctgtacag aggaaagaaa
      901 ccggtctatt ggtgttattc atgtcaaact gcgcttgctg aggctgaggt agaatacgag
      961 gataaaacat cgccttcgat atatgtgaaa ttccctttga tttcagagga attgtttgct
     1021 aagttttttg ctctttttga aaagcctgta gtgagcaaaa ctgaactaaa ggtatttgcc
     1081 ctcatctgga ctacaactcc ctggacactt cctgcaaatc tggcgctcgc ttttcatcct
     1141 gatttctcat acgtagcggc tgagatcctt cgatcaagct caggacaagc taacggagaa
     1201 gtttatattg ttgctgaagc gcttcttgaa acggtactag gcaaggatgc aaaggtcatc
     1261 caaaagtttc gggggaaaga cattgatggg ttaaaattta agcacccttt ttacgaaaga
     1321 gaatctatcg ttactctagg tgatttcgta acacttgata caggaactgg cattgttcat
     1381 atcgcccctg ggcatgggca ggaagactac gagattggga taaaatacga ccttgacatt
     1441 tatgcacctg tagatgagag agggaggttc accaaaagtg ttgagttctt tggtggcatg
     1501 tttgtctatg atgcgaacaa aaagataatt gagaaactaa aagaggtagg gaacctttta
     1561 agagaggaga agatcacaca ttcttatcca cactgttggc ggtgtaaaag tccgattatc
     1621 tttcgagcga ctcctcagtg gttcatttct atggaaaaaa ataatttaaa gtcaaaggct
     1681 cttcgagaaa taactgagcg cgtaagatgg attccggagt ggggtaggga gagaatttat
     1741 aacatgattg agaaccgtcc ggattggtgt gtttcaagac aaagagcttg gggggtacct
     1801 atagtcgttt tctattgcga ttcatgtgac catacgctga tgaacccaga tattgtcaat
     1861 tatgtagctc aaatttttga aaagcatggt gcagacgctt ggtttactca tcagacaaat
     1921 gagcttcttc cttccagcac aaaatgccct aagtgtggac atgagaattt tagaaaggag
     1981 actgatatac tcgacgtatg gtttgattcg ggtgtgagct atgcttcagt gcttgaagga
     2041 aatactaagc tttctatgcc tgcagatatg taccttgaag gaagcgacca gcatagagga
     2101 tggttccatt caagtcttct tgcctgtgtg ggaacgagag ggatcgctcc ctataaatcg
     2161 gtccttactc acggatttgt cgtggataaa gaaggggaga agatgtcgaa gtcaaaggga
     2221 aacgttattg tgccctctga tattataaaa aaatatggag cggaggttct aagactctgg
     2281 gttgtggctg aagactatag aagcgatatc aggatctcaa ctgagatatt agaaaggctt
     2341 acagagtctt acagaaagat aagaaataca tttcggtatc tacttggaaa tctctatgat
     2401 tttgaccctg taagagatgc actaggttat gaaaagcttg aggaaatcga caaatggatt
     2461 cttcatcggc ttgggatttt atctagcaag attctgaaag gttatgagga ttatgagttt
     2521 catattgttt attacgagct tcagcgtttt tgtattgtgg atttaagtgc gatttttctt
     2581 gatattcaaa aggatgttct atatacattc cctccaaatt ctgagaaaag aaggtcggcg
     2641 caaacagcca tttatactat tttaaattgc cttacaaagc tttctgctcc tgttctttca
     2701 tttacagcgg acgaggtctg gaagtatatg cccggtgaaa aaacagagag tgctcatcta
     2761 acaaattttc cagaacctga gttcattaac gacgagcttg aagagaaatg gaataggatt
     2821 cttcccataa gagatgagat attaaaggcg ctggagagag cgagaaagga taaattcatc
     2881 gggagttcac tcgaagccgg gattagtatt tatgttaaag gggagctcaa gagttttatt
     2941 caagaaaacc tcgaacttct taaaacaacg gcgatagtat cacatatagg agttgctaaa
     3001 gactcttcta agggtaataa cgtttttgag agtgtagata taccggggct ttggattgag
     3061 gtccaaaaag cccctggaga gaaatgcgaa agatgctgga cttatagaac cactgttgga
     3121 actaatccag attacccgac gttatgtgat agatgtattg ggcatttgat ggtgatggga
     3181 acctagattt tattgctaaa aaagacgttt gaagtgtgag agaatggcca gacataagtt
     3241 agaactctta gaagaaggca atccagaata ttacagactg attaatgaat caaatgagat
     3301 aatggatgaa tatagtagaa ttatgggcct agttcattct tttaaacttc ctacaacaaa
     3361 aatctggaaa tttgagttac ctaaaccaag tcttttttat agagcaaaag ccgacctatc
     3421 aataatcggt aaagaaggaa tgtctttggc aaaccgcttt ctagaatgga ataatagagc
     3481 tataactttt tgtctaagcc cacattatcg tttcagtcct gatcaagagg ttgataaaca
     3541 aacatctgtc attcacttta caaacctgct tatacatcga gcggatagac tcaacaatga
     3601 tataacatta ctccaaagta gttacaatct ccgattttca gaaatagaaa atacaatcaa
     3661 ttattggatc gctatttcag cctgggggtt taccttttta ggttttgtgg tttcagca
//
LOCUS       DATDRL010000250         2552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2936322, whole genome shotgun sequence.
ACCESSION   DATDRL010000250 DATDRL010000000
VERSION     DATDRL010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2936322"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /gene="raiA"
                     /locus_tag="VI935_08505"
     CDS             <1..431
                     /gene="raiA"
                     /locus_tag="VI935_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008289603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HLE25675.1"
                     /translation="VVVTFTVEKHRHRHRVDIDVYLKTPGGGTLHAWEESKDILMSLE
                     FAIDDIERQLNRLKERRIEQRKEIAREKVKSHTEELPSSILDLIAEERFPIAKPLGIE
                     EALMVLQDEGRFFLPFRNAETGEVNILYRKKSRKYGLISL"
     gene            628..1035
                     /locus_tag="VI935_08510"
     CDS             628..1035
                     /locus_tag="VI935_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HLE25676.1"
                     /translation="MTYKILEHTADIRMIVQADSLEKLFSEAVYGMMKIIEPKVNNIK
                     AAIQRTIALEAVDSTALLIDFLNEVLLATHIHKEVYNEVIFKSLSEHSIEATLRGFIA
                     SSLGEDIKAVTYHEADVKKKNDGTWETMLIFDI"
     gene            1288..>2552
                     /locus_tag="VI935_08515"
     CDS             1288..>2552
                     /locus_tag="VI935_08515"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HLE25677.1"
                     /translation="MFNKSNLIRIEDFLWEIPKSFRDDMRVPARIYTDDEMLDAIIVD
                     KSLEQLVNVATLPGIKGYAIAMPDIHEGYGFPVGGVAAFDSEEGIISPGGIGYDINCG
                     VRLLRSEKTFSEIKPHLRNLGRTIYHEVPSGVGVGGRLDLKGREFDMVLEKGAERMLE
                     LGYGETQDLEHIESGGVLHTAAPELVSEHAKSRGRDQLGTMGAGNHFVEVGIVETTFD
                     NGEAEKLNIFKDQVTVLIHTGSRGLGHQIATDYIRIMMKVMPKYGIVLPDRELACAPF
                     KSREGQEYFKAMSAGANFAWANRQLITWEVRKAWKDVLGKEGGKLSMVYDVAHNIAKI
                     EEYTLDGKRYNLVIHRKGATRSFPGQPVLIPGSMGTNSYILVGQEKAMISSFGSSCHG
                     AGRRMSRTRARKEVSVEELRQRLESMGIVV"
BASE COUNT          809 a          454 c          601 g          688 t
ORIGIN      
        1 aagtggttgt aacctttaca gtagaaaagc ataggcatcg tcatcgtgtt gatattgatg
       61 tatatcttaa aacacctggt ggcggaacgc tccatgcatg ggaggaaagt aaagatatcc
      121 ttatgtctct tgaatttgcc attgacgata tagaaaggca gctcaatcgg ctaaaggaaa
      181 gacggattga gcaaagaaag gagatcgcca gagagaaggt aaagagtcat acagaggagt
      241 taccctcttc tatcctcgat cttatagcag aagaaagatt ccctatagcg aaacccctcg
      301 gtatagaaga ggctttaatg gttcttcagg acgaaggaag gttctttttg cctttcagaa
      361 atgctgaaac tggcgaggta aacattttat ataggaaaaa aagtagaaaa tatggcctca
      421 tttcacttta ggaaatgata ccacgtaaca agctcttcaa atggagctaa tttaaagtaa
      481 gcttcgatta ttgttatatc ttgtttagat cgtattaact ttagtataac tatgtttact
      541 cacaataagc cttaatacta agcaaccgga gaaaaaccag gatttttaga ttatcataag
      601 aacgtacctt ttatttagat accacatatg acatataaaa ttttagaaca caccgccgac
      661 attaggatga ttgttcaagc ggattctctc gagaagcttt tttcggaggc tgtctatgga
      721 atgatgaaaa ttatcgaacc gaaggtcaac aacataaagg cggccataca gagaactatt
      781 gcccttgaag cagtggattc aaccgcgctc ctcatagatt tcctcaatga ggttttactc
      841 gcaacacaca tacataaaga agtctacaat gaggtgattt ttaagagctt atccgagcac
      901 tcaatagaag cgacgctgcg tggctttatt gcatcatcgt taggtgagga tattaaagct
      961 gtgacctatc atgaggcaga tgtaaaaaag aagaatgatg gaacgtggga aaccatgctc
     1021 atttttgata tttagcatgg aaatcgcctt ggtgttgctt gatgctggat gtactattta
     1081 actgcagata cgcctttttt agcgggcaat ttttctccaa ataattgtca ttaatttatg
     1141 aaacgatgtg tatcaaattt aatggcatat aatttgcata gattcatgat gtatattcaa
     1201 attcttaaca aattttaata aagagaatca aattatctaa attaggctta tgcatacaga
     1261 taacttcatt aaggtgaaat aacgctcatg tttaataaat cgaatctcat aaggatagag
     1321 gattttcttt gggaaattcc aaaaagcttt cgtgatgaca tgcgggtgcc agctcgtatc
     1381 tacacagatg atgaaatgct cgatgctatt attgttgaca agtctttaga gcagcttgtt
     1441 aatgttgcta cactcccagg aatcaaggga tacgccattg ccatgcctga tatacatgag
     1501 ggatatggtt tccctgttgg cggagtagcg gcctttgaca gcgaggaagg tattatctct
     1561 cctggtggta tcggctatga cattaattgt ggagtaagac tcttacgctc agagaaaacc
     1621 ttctcagaga ttaaacctca cctccgtaat cttgggagaa caatttacca cgaggttccc
     1681 tcaggcgtag gggtaggagg gaggctcgat ttgaaaggta gagaattcga tatggttctt
     1741 gaaaaggggg cagagcgaat gctcgaactt gggtatggag agactcagga cctcgaacat
     1801 atagaatcag ggggtgtttt acacaccgca gcgccagaac tcgtttcaga gcatgcaaaa
     1861 agcagaggtc gggatcagct tggaacaatg ggggcaggca accactttgt ggaggttggg
     1921 atcgttgaga caacctttga caatggggaa gccgaaaaac tcaatatttt taaagaccag
     1981 gtaacagttc tgattcatac cggttctagg gggcttgggc atcagattgc aaccgactat
     2041 atacggatta tgatgaaggt tatgcctaaa tatggcattg ttctgcctga ccgtgaatta
     2101 gcatgtgccc catttaagtc aagagaggga caagagtact tcaaagcgat gtcagcaggt
     2161 gcaaacttcg catgggcaaa ccgacaactc attacatggg aagtgagaaa ggcgtggaaa
     2221 gacgttttag gcaaagaggg agggaaactt agcatggttt atgatgtagc acacaatatt
     2281 gcaaaaatcg aagagtacac attagatgga aaaagatata atcttgttat tcaccgaaaa
     2341 ggcgccaccc gttccttccc aggtcaaccg gtacttattc ccggaagtat ggggacaaat
     2401 tcttatattc tcgttggaca agaaaaagca atgatttcaa gctttggatc gagctgtcac
     2461 ggcgcaggaa gaaggatgtc tcgcacacgg gccaggaaag aggtaagcgt agaagagctt
     2521 agacaaaggc ttgagagcat ggggattgtc gt
//
LOCUS       DATDRL010000251         7537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2196576, whole genome shotgun sequence.
ACCESSION   DATDRL010000251 DATDRL010000000
VERSION     DATDRL010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196576"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1752)
                     /locus_tag="VI935_08520"
     CDS             complement(142..1752)
                     /locus_tag="VI935_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLE25678.1"
                     /translation="MNFRLDRKLTFPTGTAFLNSWTIQRPVFCVGAYEVYRIQGGSEI
                     LFGLVIRCRKREEALEQSKQHLTWWRLQGEDRIEVLANDIRMQGEWYLVLACIAIPNK
                     IVLFEELGTTLLTQVQAESLIIDLIALCKLAHTHSVNPVIEIGSVLLVKHKGGIKSIP
                     LFVKISDLPAETEAVESIAKIVYFFTTGIDTSTFTSIPSNKRTTPPPASRWNKNIDHG
                     LSSLLGSCLDSKDPQRVISLSDFEIRLSHFSGQGGAQTTIGIQRNQESVEHLGSKTSK
                     GLAKVAGMSALKELLIEEVVHPIKDPEPYKRYGLTIPNGILLFGPPGCGKTFIARQLA
                     EELDFYFLEIIPSEIASPFIHDSVLKIRDIFATAEQHAPSIIFIDEFEALVPSRHELS
                     GHQQYKSEEVNEFLVHLNECVSKNILVLAATNEPDKIDGAILRPGRMDKKIRVDPPDY
                     EARIELLKMYLSHRPLGNVEIEKFARLLEGYSCVDIRNICDESARLAFKQDSLIENEH
                     LSEAIHRNPSSVSVGDLLKYEKFQQSGI"
     gene            complement(1755..4280)
                     /locus_tag="VI935_08525"
     CDS             complement(1755..4280)
                     /locus_tag="VI935_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HLE25679.1"
                     /translation="MSDTINYGIDLGTTNSAIAKCEGDAVRVFKNRDQMDVTPSVVRI
                     EKTGRIIVGRRAYQTLFSDTDNVAAEFKRWMGQSDRKVFKAATKSLSAEDLSAEILKS
                     LLEDARRQTTDPITASVITVPSAFGQLQCEATARSAALAGLTEAPLLQEPLAASIAYG
                     MKLDAWDKRWLVYDLGGGTFDIALVSTRDGQLSVLEHRGDNMLGGKDFDRLIVERIFW
                     PRLEDLFNMPGPDSDPAIRRKLIQILRGKAEEAKIDLSLSERVVVSIFDAGNDEDGNP
                     IEAEFEITRAELNPLVEPLIAKTIELCKQALDKARITTKDISTIILVGGPTHMPILRE
                     MLTTEVSVPLDFTIDPMTVVARGAAIYASTLPCKQATLKPIKSEAINIKLAYETVWSE
                     TTCLVAGRMNNLPQNIDSIQISIEAETGHWNSGWLPVQKDYFETKVHLLEGRTNRFWI
                     YLRDRKGNDLAPSPDSFSIRHGLTLAEPPLPHSIGAEVVRPDGKSEIDVIFPRSTPLP
                     AQKIVPYKAYKKLRPSQTEDYLAIKIWEGESFSDPEANTFVGVLKIVSNEIRRPIPEG
                     TDIEVSISINASRLMEVQAFVPVLNQHFQNRVYVPKENEEVIVEKAQEVEHELNEHYD
                     RIENLEEVAQEIDDPTIQQEVNELKVKLDELTEERYRLESHNQSDPDDAKRVVQQSKE
                     VRGRLSEIERRLKSQARLPLLLRNVEAKRARTEEVVKRWGDRLEQKEYELLCREANRQ
                     IEREDEKALEKVIRDFESLKWRVLFKQDWYWKELFDSLCQPGVPFINAQEAQRLITVG
                     QEATARGDGDRLREIVRELWKLQPRSAVETEKEKALRAGIRRN"
     gene            complement(4280..4663)
                     /locus_tag="VI935_08530"
     CDS             complement(4280..4663)
                     /locus_tag="VI935_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25680.1"
                     /translation="MADQKDLIELPRSIIATLALEIWRLWRTVENKESHANSVNLRYS
                     IRKMKEALEVQGCSFIDLTGEIYDAGMAVDIIDTEGERKDGVTDLIIKEMVAPIILFG
                     GVLLTHGQVILERSAKGENIAKESK"
     gene            complement(4664..6403)
                     /locus_tag="VI935_08535"
     CDS             complement(4664..6403)
                     /locus_tag="VI935_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25681.1"
                     /translation="MRCIERTSYQENAFRILGVSANATLKTLRDAYNTLKQRGKLGGL
                     GLVDDPLCFLSNIERSEPNVRDAFNKLQNPQIRLKERLFWFTNSTQVDRDALVWLVSK
                     ELTKAIQIWESDQNYTTLANLARLRHAICIFKDPNVQDTHLWMKALGSWRIIIESDDF
                     WKHFTDIEMDSGFEPSAIVSDIEILREDSWELVLKPSIVLAQNAINEAKYDVVRRHLD
                     LIRGSGFPSLIVHHIEGNILEPLESRFEETKAKILKTLSDLLEDKEASLETKRKAGDQ
                     AYNFYKVDLFTQVHRLTQVAGVESEAATRAHQAAADCLRSISIFYHNHAQAYQISEQL
                     LKEAQSLAPDSIISERIKEDLEIVSKSAQQENIWKDPKPMTSFDKWLIWVMLLLIFLI
                     FPLISPLFFNSQSSYSNKPGLYSSPTHTVPYSTQKRSPQNNPDDNPFIDYEQNKKKKN
                     PFLEYEKSIESEKRIETAKVEQLKTEIEDAKLRLGNQELELRELADKIGSYLTQIEQY
                     ATRIKQIESDIDSGYSINQDEYETALARHNFYVDLYNTELAQHNLKYAEYERLFKETN
                     AKISEHNKMITGR"
     gene            6814..>7537
                     /locus_tag="VI935_08540"
     CDS             6814..>7537
                     /locus_tag="VI935_08540"
                     /inference="COORDINATES: protein motif:HMM:NF022633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HLE25682.1"
                     /translation="MPNILYHYTSKIGLLGIIKDKCLWATNILYLNDAAEISYTLQLG
                     AEVLETKMKNPKLKDNELKLIHKMYEVVKGRLEFGIPNTYVACFSEVDDSLSQWRAYC
                     PKTGGFSIGFDYEYLRSLASDQNYELKECVYDEKPELPSNKSRGLKEVERIIDKHSKE
                     ATNWTLNGKDLDVDTGDRIVFDFLYELAYVATWMKHEAFKEEKEWRVISSIHNVDVSS
                     VFHREGTSMIVPYREFKLVKDDQ"
BASE COUNT         2035 a         1636 c         1516 g         2350 t
ORIGIN      
        1 attctgagtg agaaatcggc agagtctcta gaggcagaac aggacagaac tctgtttact
       61 gtgcatcaac ggggtcaggt ctcaactttt aactttcatt ttctccttat gtcaaatgtt
      121 gagacctgac cccaaatttt gtcatatccc actttgttga aatttctcat attttaataa
      181 atcacccacg gatacacttg acggattccg atgaattgct tcggacaagt gctcgttttc
      241 aataaggcta tcttgcttaa aagctaatct cgctgactca tcacaaatat tccgtatgtc
      301 gacacatgaa taaccctcga gtaaacgggc aaatttctca atctcaacat tgcccaatgg
      361 cctatgagat aaatacatct tcaacaactc aattcttgct tcatagtctg gtggatctac
      421 acggatcttt ttatccatcc gaccgggacg caggatagcc ccgtcgattt tatcaggctc
      481 gttagtggct gctaagacga gaatgttctt actgacgcac tcgtttaaat gaacgagaaa
      541 ctcgtttacc tcctctgatt tgtattgttg gtgtccgctc aattcatgtc gagatggtac
      601 taatgcctcg aattcatcaa taaaaattat ggatggagca tgttgttcgg ctgtagcaaa
      661 tatatcgcgt attttcagga cactatcatg aatgaaagga cttgcaattt ctgagggtat
      721 tatttcgaga aagtaaaaat ccaattcttc ggcaagttgt cttgctataa aggttttacc
      781 acatccaggt ggcccaaaga gaagaattcc gttaggtata gtaaggccat aacgcttgta
      841 tggctctgga tcttttatag gatgaacgac ctcttcaata agtaattcct tcagcgccga
      901 cataccggcg actttggcaa gtccctttga agtttttgaa cctaaatgct ccacggattc
      961 ttgatttcgt tgaattccta ttgttgtttg ggctccaccc tgtccagaaa agtggcttaa
     1021 acgtatttca aagtcgctga gagaaatcac tctttgcggg tctttggagt caagacatga
     1081 tccaagaagc gatgataatc cgtggtcgat atttttattc cagcggctag cagggggtgg
     1141 agttgttctc ttattagagg gtatagatgt aaatgtagag gtgtcgatgc ccgttgtaaa
     1201 gaaatagacg atctttgcaa tagactctac agcctccgtt tctgctggca aatccgaaat
     1261 ttttacaaaa agaggaatgc ttttaattcc ccctttatgc tttaccaaca acacgctccc
     1321 gatctctatt accggattaa cactgtgtgt atgagctaat ttgcacagag caatcaaatc
     1381 gattataagt gattcggctt ggacttgtgt caggagagtt gttcccaact cttcaaataa
     1441 cactatcttg tttggaatcg cgatgcaggc aagaaccaaa taccattcac cttgcatgcg
     1501 aatatcattc gctaaaacct ctatcctatc ttcaccttgt agccgccacc aagtcagatg
     1561 ttgtttgctt tgttccaaag cctcctcccg ttttctacaa cgaatcacaa gtccgaaaag
     1621 aatctctgaa ccaccttgga ttctgtaaac ctcataggcc ccgacacaga aaacaggacg
     1681 ctgaattgtc catgaattaa gaaaggctgt accagtaggg aaagtcagtt ttctatcaag
     1741 acgaaagttc atacctaatt cctccgaatt cccgctcgca aagccttttc tttttcggtc
     1801 tcaacagcac tcctaggttg cagtttccat agctcccgta caatttctct caatctatct
     1861 ccatctcctc gtgctgtagc ttcctggcct actgtgataa gtcgttgtgc ttcttgtgca
     1921 ttgataaaag gaacgcccgg ctgacacaaa gaatcaaaca gttctttcca ataccaatct
     1981 tgcttgaata gaacgcgcca ctttaaagat tcaaaatcac ggataacttt ctccaaagct
     2041 ttttcgtcct ctctttcaat ttgacgatta gcttcacggc ataacagttc atattccttt
     2101 tgctcaagcc gatctcccca tcttttcaca acttcctctg ttcttgcccg ctttgcttcg
     2161 acattgcgga gaagaagtgg taatctggct tgcgatttaa ggcgtctttc aatttcactt
     2221 aatcgaccac gaacctcctt ggattgttgg acaactcttt tcgcgtcgtc tggatcactc
     2281 tggttgtggc tctccaaacg gtaccgttcc tcggttagct catctaattt cacttttagc
     2341 tcatttactt cttgttgaat cgtagggtcg tcgatttctt gagctacctc ttctaaattt
     2401 tcgatacggt cataatgttc attcagttca tgctcaacct cctgtgcctt ctcaactatg
     2461 acttcctcat tctccttagg cacgtagacg cgattttgaa agtgctgatt tagaacagga
     2521 acaaaggcct gtacctccat aagtcgggaa gcattgatcg aaatggacac ctcaatatct
     2581 gtcccttcgg gtataggacg gcgaatttcg ttggaaacaa tcttaaggac tcctacaaaa
     2641 gtattggctt cgggatcaga aaacgactcg ccttcccata ttttgatagc taggtaatcc
     2701 tcagtctgac ttggcctaag ctttttatac gctttatagg gaacaatttt ctgggcgggg
     2761 agcggagttg agcgtggaaa gatcacgtca atctcacttt taccatcagg acggacaact
     2821 tctgctccaa ttgaatgtgg cagtggtggt tctgcaagcg ttaaaccatg acgaatggaa
     2881 aaggaatccg ggcttggggc gagatcattc cctttcctgt cacgtaagta gatccagaaa
     2941 cggtttgtcc tgccttctag aagatgaact ttggtttcaa agtaatcttt ctggacaggt
     3001 aaccagccac tattccaatg gcctgtctct gcctctattg atatttggat ggaatctata
     3061 ttttggggta gattattcat tcgaccggca accaggcaag ttgtctccga ccacactgtt
     3121 tcgtatgcaa gtttaatgtt aatggcttct gactttatgg gttttaaggt tgcttgtttg
     3181 caaggtagtg tagaagcata tatggctgca ccacgtgcca caacagtcat tggatcaatc
     3241 gtaaaatcta gcggtacact gacttcagtt gtaagcatct cacgaaggat gggcatatgc
     3301 gtaggtcctc cgacaagaat aatcgttgat atatcctttg tagttatgcg tgctttatcc
     3361 agtgcctgct tacataactc aatcgtctta gcaattagag gctcaaccag cggatttaat
     3421 tcggctctag ttatttcaaa ctcagcctct attgggttac cgtcttcgtc atttcctgca
     3481 tcaaaaatgc tcacaacaac ccgttcagag agactgagat cgatttttgc ttcttcggct
     3541 ttaccgcgaa gtatctgaat aagttttcgc cgaatagccg ggtcgctgtc aggcccaggc
     3601 atgttgaata aatcttccaa acgtggccaa aaaatccttt ctacaataag tcggtcgaag
     3661 tccttgccac caagcatatt gtcacctcgg tgttccaaga ctgacaattg tccatctctg
     3721 gtggaaacaa gggctatgtc aaatgtaccc cctccaagat cgtagactag ccagcgcttg
     3781 tcccaggcat ccagtttcat cccataggcg attgaagccg ccaaaggctc ttgtaaaagc
     3841 ggagcctcgg ttaaccctgc taatgcggca gaccgggctg ttgcttcgca ttgcaattga
     3901 ccgaaggcag atggcaccgt aatgaccgac gctgtgattg ggtctgttgt ctgtctccgg
     3961 gcatcttcga gaagtgattt cagaatttcg gcggagaggt cttcagcaga aagcgatttg
     4021 gtagcggctt taaagacttt acgatcgctt tgacccatcc aacgtttaaa ctccgcagca
     4081 acattgtctg tgtctgagaa cagggtttga taagctcgcc ggccaacgat gatgcgaccc
     4141 gtcttctcaa tacgcaccac agaaggcgtt acatccatct ggtcgcgatt cttgaaaacc
     4201 cgtactgcat caccttcaca cttagctata gccgaatttg tggtcccaag gtcaattcca
     4261 taattgatgg tgtcgctcat tatttgctct cctttgctat gttctcacct tttgcgctcc
     4321 tttcaagtat aacctgtcca tgggttaaga gtacgccacc gaagagtata attggagcca
     4381 ccatctcttt aatgataaga tctgttaccc catcttttct ttcaccttca gtatctataa
     4441 tatcaacagc cattcctgcg tcatatatct ctcctgtcaa gtcaataaac gagcaacctt
     4501 ggacttcaag agcttccttc atcttcctga ttgaataccg gaggttcaca gaattggcat
     4561 gactttcttt attctcgaca gtgcgccaaa gccgccaaat ctcaagtgca agagttgcaa
     4621 taatgcttct tggcaattca attaaatctt tttgatcagc catttatcgc cctgtaatca
     4681 ttttattgtg ttcactaatt tttgcattgg tttctttaaa taatcgctcg tattccgcgt
     4741 atttaaggtt atgttgagcc aactctgtgt tgtacaaatc cacataaaaa ttatgacgag
     4801 ccaatgcagt ttcatattca tcttgattga ttgaatagcc agagtcaatg tcgctctcga
     4861 tctgtttgat ccttgtggca tattgttcaa tctgggttag gtaggatcct atcttgtcag
     4921 ccaattctct gagttccaat tcttgatttc ccagccttag tttcgcatct tcaatctctg
     4981 tttttaattg ttctacttta gcagtttcta ttctcttttc agactcaatg gatttttcat
     5041 attcgagaaa tggatttttc ttttttttat tttgttcata atctataaat ggattatcat
     5101 cgggattatt ttgtggcgat ctcttttgcg tagaatatgg aactgtgtgt gtcggacttg
     5161 aatacagacc gggcttattt gaataactag attgactatt aaagaataac ggagagatta
     5221 acggaaagat taaaaagata agtaatagca ttacccaaat aagccactta tcaaaagagg
     5281 tcatcggctt tggatctttc cagatatttt cctgttgtgc actcttggaa actatttcca
     5341 gatcttcttt gattctttca gaaataatac tgtccggtgc taaactctgt gcttctttta
     5401 acagttgttc cgaaatttga tacgcttgag catgattatg gtagaagata gatatacttc
     5461 taagacaatc cgctgcagct tgatgcgccc gtgtggccgc ttcagattct acacctgcca
     5521 cttgagtcaa gcgatggact tgtgtgaaga gatcaacttt gtagaaattg tatgcttggt
     5581 cgcctgcctt acgttttgtt tctaaagagg cctccttgtc ttctaataaa tcggaaagtg
     5641 ttttaagaat tttggctttg gtctcctcga atcgactctc taagggttct aatatattcc
     5701 cttcaatatg gtgtacaata agcgatggga aaccagaacc tcgtattaaa tcaaggtgac
     5761 gccttacgac atcatatttg gcttcgttaa ttgcattctg cgctagaaca attgatggtt
     5821 taagaacgag ctcccaagaa tcctcgcgta gtatctcaat atcagacact attgcagaag
     5881 gttcaaaccc ggagtccatc tcaatatcag taaaatgttt ccaaaagtcg tcgctttcaa
     5941 taattatgcg ccaacttccc aatgctttca tccacaaatg cgtgtcttgg acattaggat
     6001 ctttgaaaat acaaatggcg tgacgcagcc gcgcaagatt agcaagcgtt gtataatttt
     6061 gatctgactc ccaaatctga atagcctttg tcagttcttt tgaaactaac cagactaatg
     6121 catccctatc aacctgtgtg ctattagtaa accagaatag cctttctttc aaacgaattt
     6181 gcggattttg taatttattg aaagcatcac gaacgtttgg ttcacttctc tcaatattac
     6241 taagaaaaca taaaggatcg tctaccaagc caaggccacc gagcttgcca cgttgtttta
     6301 gtgtattata agcatctcgt agcgttttca gggtggcatt cgctgaaaca cctaaaattc
     6361 taaaagcatt ttcttgataa ctcgtcctct ctatacaccg cattattcta actccccccc
     6421 tatttcccca ttcatggatg aactcctctc ttaatacaat tatctcatca cggctaatcc
     6481 cttgtcaaca cacgtaaaga aggcaagggg gtgaatgggg taccgaactg ggcaagctgg
     6541 tctacttcca ctcccgataa gggcctaact gggcagttac gagttgtcag aaaatatctg
     6601 atgacataag gtagttatga attttagggt ataaggttat agaatgtcgc ttctaaacat
     6661 tgttgaaatt agccgcacta aaggaagatg ggttcaattt ttttgtttaa aagttacacg
     6721 tgtcttgtag ctttatatag agcttgagcg tagatacagt tatagggtat attccttcca
     6781 tcaagtttag aaacttaaaa gcaatcttat tggatgccta atatcctata tcattacaca
     6841 tctaaaattg gcttacttgg tattatcaag gataagtgct tgtgggcaac aaatattctt
     6901 tatttaaatg acgctgctga aatttcttat actctccagt tgggcgctga agtgttggaa
     6961 actaaaatga agaatcccaa actcaaagac aatgaattaa aactaatcca taaaatgtat
     7021 gaggtagtga aaggtcggct ggaatttgga attcctaata cctatgtagc ttgtttctcc
     7081 gaggttgatg actccttgag tcaatggagg gcttattgtc cgaaaactgg cggctttagc
     7141 attggttttg attatgagta tttgaggtct ttagctagcg accagaacta tgagctaaaa
     7201 gagtgtgtct atgatgaaaa gcctgaatta ccttcgaata aaagcagggg actgaaagag
     7261 gtagagagga ttatagataa acatagtaaa gaggctacaa attggacact taatggaaaa
     7321 gacttggacg ttgataccgg tgacaggatt gtttttgatt tcctctatga acttgcctat
     7381 gtggcgactt ggatgaaaca tgaggcattt aaggaagaga aagagtggag ggtgatttct
     7441 tcaattcaca acgtggatgt atctagtgtg tttcatagag aggggacatc aatgatagtt
     7501 ccatatcggg aatttaaact tgtaaaggat gatcaac
//
LOCUS       DATDRL010000252         2792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_941631, whole genome shotgun sequence.
ACCESSION   DATDRL010000252 DATDRL010000000
VERSION     DATDRL010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941631"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(829..2157)
                     /locus_tag="VI935_08545"
     CDS             complement(829..2157)
                     /locus_tag="VI935_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015792038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HLE25683.1"
                     /translation="MELIEKFFGFREKGTSFKTETIAGLTTFMTMAYIIFVQPAVLSQ
                     AGMDFGAVMMATCLSAAIATLIMGLYANYPIGVAPAMGENFFFVFTVVLGMGLAWQKA
                     LGAVFISGVLFVVLTAFRIRELIVDAVPQALKDSIAGGIGLFISFIGLLQAGIIEKST
                     EGILSLGDLKKEPVLLSIFGLIFTAVLMSRKITGAILIGIIVSTILGLLLGIVNYPGS
                     VLSLPPSILPTFLKVDIKGALEIDFITVTIVFLFMVMFDTIGTLIGVAGIAGFLRNGK
                     LPRAGRALMADAVGTSVGALLGTSTVTAYIESTTGVSQGGRTGFVCVVIAILFLLSIL
                     FFPLVKMIGGGYEISKGVFIYPLTAPALVIVGSLMIQTIKNVRWDDFTEGIPAFLTLI
                     GIPFTFSIADGLCFGFISYPILKLLSGKGKTVSWLIYLLGALSIMRFLFA"
     gene            2515..>2792
                     /locus_tag="VI935_08550"
     CDS             2515..>2792
                     /locus_tag="VI935_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25684.1"
                     /translation="MSSLHSKRSLLTLQHDGLQDELSPEENINHKLKALCTLHSDLPS
                     PESMNPVEVALYTTDQFSLEEDEASTTRFNAIYYSFKYHFEHNAFYKR"
BASE COUNT          908 a          606 c          541 g          737 t
ORIGIN      
        1 ggacgaagca atccgaccac ttagtgtcgt cctgagccta agcgaaggat cccatgataa
       61 ctcacgagat ccttcagtcg ctttgctcct tcaggatgac actttgtgtc agattgcttc
      121 acggagcctg cactgaacga agtgaacgtg ttcgcaatga caatacacta ttgtggagac
      181 cccgtaccaa tctactggaa attatcaagt taaaacttag cctaacagca aactacaggg
      241 tatcagcata taaacagata cccacttcag tcattgcaat gactataggc ggagtttact
      301 aaccacaacc gctaatgcgg gactggaaag tcccgcctat ctttggtgga ttgcagttta
      361 ttaggtaagc agatttgcaa gatacagata ggcggggttt tctaaccccg cattctgatg
      421 ccggtctatc gtacatattg tatatgtggg acaagaacgt tatttacacc atatagaaca
      481 acaacatacc tataggcgta gttttccaac cacgtcttaa tgctgaacaa gaaagtccag
      541 cccatccaac atggttaata aacaaggcga tagtcggttt tttccaacac cgaccgtcga
      601 tgcgggataa aactgtccga gccgaagtcc tgaggccttc gatgcctcag ggtaaactcc
      661 gcaaaggagc ggcaccaagt ctaagcgaag ggactggaga aggagcagtg agtaatctca
      721 aagggattgc ttcgctacca ctcgcaatga catcgcggaa ttgtcatttc attcgcaatg
      781 acaactgaaa cactttagca agctacatgg aattacctag ttaaaggatt acgcaaataa
      841 gaagcgcatt atggaaagcg ctcctagtaa atatataagc caggaaacgg tcttgccttt
      901 acctgaaagc agttttaaga tgggataaga gataaagcca aagcaaagac catcggcaat
      961 actgaaggtg aatggtattc caattagtgt taggaatgca ggaatccctt ccgtaaaatc
     1021 atcccacctt acgttcttaa ttgtttgaat cattaaactt cctacaataa caagtgctgg
     1081 agcggtaagg ggatagataa aaacaccttt agagatttca taacccccac ctatcatttt
     1141 tacaagcgga aaaaacagaa tggacaacaa aaaaagtata gcaataacta cacatacaaa
     1201 tcccgttctg ccaccctgag aaacccctgt tgtgctctca atataagcgg taacggttga
     1261 tgtgcctaag agcgctccca ctgaggttcc tacagcatca gccattagcg ctcttcctgc
     1321 tctaggaagc ttcccatttc ttaaaaagcc agctattccg gctaccccta ttaatgtgcc
     1381 tattgtatcg aacataacca tgaaaaggaa aacaatagtg acagttatga agtcaatttc
     1441 aagagcccct tttatatcaa ctttgaggaa tgtaggtaaa atagatgggg gaagggaaag
     1501 aacgcttcca ggatagttaa ctattccaag aagaagcccc agaatagtcg aaacgataat
     1561 tcctattaat atagctcctg taatctttct tgacatcaaa acggcagtaa atattaatcc
     1621 gaagatagaa agaagcaccg gttctttttt taaatcgcct aaacttaaaa tcccttcagt
     1681 actcttttca ataattcctg cttgaagtag tcctataaat gaaatgaaca atccaattcc
     1741 acctgctatt gaatccttga gtgcttgagg aacagcatct acaattagct ctcgaattct
     1801 aaaagcggtt aagacaacaa aaagaacccc tgaaataaaa accgctccaa gggccttctg
     1861 ccacgctaag cccataccga gtacaactgt aaagacaaaa aagaagttct cccccatagc
     1921 aggagcaacc ccgataggat agttggcata taaccccatg ataagggttg cgatagcggc
     1981 agacaggcaa gtagccatca ttacagcacc aaaatccatt ccagcttgtg agagaacggc
     2041 aggttgtaca aatattatat aagccatggt catgaatgtg gtgagaccgg caatggtttc
     2101 ggtcttaaaa gatgttcctt tctctcgaaa accaaaaaat ttctctatca attccatttg
     2161 ttgtaataat caattaaatt tggtaattgc ctcttaatct tatatacatt ttcttaaatc
     2221 gccacagagt acacggagaa ctgagatagg tatttctata aagaatctct caatctgctt
     2281 tgtgacctat gtgccacatg ttgtcgttgg aacggattaa accggggtgt atctataaat
     2341 gattgatccc tactcgatcc ccgaagaatt cataggggac acgtggatga gaaaggaatt
     2401 tttatgtaac ctagctcgct ttcttgacaa aatctttatt ttttaataag gtaatcaagg
     2461 gggaaagctt gcctatattt tataaaattt cgagctaact taaagaggtt tatattgtca
     2521 tctttacatt caaaaaggtc tctcttaact cttcagcatg atggtctgca agatgaactt
     2581 tcacctgaag aaaatatcaa tcataaacta aaagcccttt gcaccttgca cagcgaccta
     2641 ccatcgcctg aaagcatgaa cccggtggag gttgctttat acacaacaga tcaattttcc
     2701 ttggaagaag atgaagcaag tacaacccgc tttaatgcta tatactatag tttcaagtat
     2761 cacttcgaac ataatgcttt ctataagcgt tt
//
LOCUS       DATDRL010000253         3540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3016058, whole genome shotgun sequence.
ACCESSION   DATDRL010000253 DATDRL010000000
VERSION     DATDRL010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3016058"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /gene="bamA"
                     /locus_tag="VI935_08555"
     CDS             complement(<1..1610)
                     /gene="bamA"
                     /locus_tag="VI935_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HLE25685.1"
                     /translation="MRTKSPYVFFFRILITLFFTLSLTRIGFCQENIIRIDIKGNRRI
                     ETDLIRINISAKVGDLLSQETVSTDIKKIYKLGFFDDVSAVVEKNPEGVILIYNVKEK
                     PVVVDLRVKGNKKINTDEILKAIEVKEGRIIELEKVKMSAAAIKKLYLDKGYVAAEVN
                     YDIEPRTEGTVGVTFDIKEGKKAFIKEVIIVGNKTIKTKKIKGVMQTKPKWFLTFITE
                     RGIYRQEELDRDSERIRALYLDNGYIDVKVAKPEVQYSEKKEGFVVTYRIEEGNQYQV
                     KDIKVEGDLVASEDELMKLLTLQIGKPFSRAAIGEDISRITTFYGDKGYAFANVDPDF
                     KIDRENLKVDISFKIEKGKEIYIRNIDIAGNIRTRDKVIRREIPIQEQQLFSSSRIQA
                     IKPRIFRLGYFEENIEVSTDRVPDTEDQLDISVKLKEKPTGFFSIAGGFSSVETFIFA
                     GQIQESNLFGYGNSLSLSAQIGGVTRLFLLNYTDPHFLDSDWTLDVLGFQTDREYQDF
                     SRFSYGGSVTLGRRLLRDLTGRITYRLE"
     gene            complement(1600..2283)
                     /locus_tag="VI935_08560"
     CDS             complement(1600..2283)
                     /locus_tag="VI935_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLE25686.1"
                     /translation="MSKEIIKAKDLTKVFETDGSRVTALKGLNLVINKGEVVGIVGVS
                     GSGKSTLLHILGTLERPSDGEVLYQGENVFMRDDDELALFRNREIGFVFQFHYLLSEF
                     SALENVMMPLLIQGIQLNEAREIAAIILRRVGLGERLRHRPGELSGGEQQRVAIARAV
                     VLKPKVILADEPTGNLDLETGNSVLDLFLELNVEVGITLALVTHNPMIAMRLGRRIRL
                     SDGKIVDEN"
     gene            complement(2280..>3540)
                     /locus_tag="VI935_08565"
     CDS             complement(2280..>3540)
                     /locus_tag="VI935_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLE25687.1"
                     /translation="SKRRQTFISVIGLISILGVFMGVMALNVVLAVMRGFEEELRDKI
                     LGVNSHLVVLSYDGPMGDYERIKDKIINTNGVVGTSPFIYGQGMVVGENNVLGAVIRG
                     IDPKNAGSVTNIEEALGRGMVGRNGKVKTDELREIGRELITKLNRETKSGKPPLILGI
                     ELASNLGISAGDQVNLVSRFGKVGPFGPQAKIKRFEVVGVFDYGMVEYDSSIAYVGLR
                     DAMDFFEMNSKVTGVEAKVRDLYKAREIGEKIAVSLGFPFYTRNWEEVNRSLFRALRL
                     ERIAIAIFLALIILVAALDIVSTLTMVVMEKGKDIAILRAMGATRRGIMRIFITDGMI
                     IGFVGTLLGSIAGLGICYLVKTSDAVKKLIPFDTQVYPVSEFPVKIEPLYFLGVAIMS
                     LGICFLATLYPSYQASRKDPVEALRYE"
BASE COUNT          991 a          883 c          529 g         1137 t
ORIGIN      
        1 tgttcgagtc tataggtaat cctgcctgtt aaatccctta aaagccttcg gcctaaggtt
       61 acacttcctc catatgaaaa cctactgaaa tcctgatatt ccctgtcagt ttggaaccca
      121 aggacatcaa gcgtccagtc ggaatctaag aagtgtgggt ctgtataatt caatagaaaa
      181 agccttgtga caccacctat ttgagcagaa agagataaac tattcccata gccaaagaga
      241 ttcgattctt gaatctgacc agcaaatata aatgtctcaa cagagctaaa accaccggct
      301 atgctaaaaa acccggttgg tttctcttta agcttaacac ttatatcaag ctgatcctct
      361 gtatcgggga cacgatcggt ggaaacctcg atattctctt caaaataacc cagcctaaat
      421 atacggggtt ttattgcctg aatccttgat gaactaaaaa gctgctgttc ttgtattgga
      481 atctctcttc ttataacctt gtcacgggtt cttatgtttc cagctatgtc aatatttctt
      541 atatatatct cctttccttt ctctatctta aagcttatat caactttaag attctcccta
      601 tcaatcttaa aatctggatc aacgttagca aaagcatatc ccttgtcacc ataaaatgta
      661 gtaattcgtg agatatcttc tccaatagcg gcacggctaa atggtttacc gatctggagt
      721 gtgagtagtt tcatcaattc atcttctgaa gccaccaagt ccccttctac tttaatatct
      781 ttgacttgat actgattacc ttcttcaatc ctgtaggtaa caacgaagcc ttcttttttt
      841 tcgctgtatt gaacctcggg cttagcaacc tttacatcta tgtaaccatt atcaaggtat
      901 agtgctctaa tcctttctga gtctctatcc aactcttcct gcctataaat ccctcgttca
      961 gtaataaaag taaggaacca tttaggtttg gtctgcataa caccttttat ctttttcgtc
     1021 tttatagttt tattacctac aattattacc tcctttataa atgccttctt gccctctttg
     1081 atatcaaaag taacaccgac cgttccctct gtccttggtt caatatcata gtttacctcg
     1141 gcagctacat atcctttatc caaataaagc ttcttaatcg cagccgcact cattttaacc
     1201 ttttccagtt caattattct tccttcctta acttctatag ccttgagaat ttcgtctgta
     1261 tttatctttt tgtttccttt tactcgaagg tcaacaacaa caggcttctc tttaacattg
     1321 tagataagaa ttactccctc aggatttttt tcgaccacag ccgatacatc atcaaaaaaa
     1381 ccgagtttgt aaatcttctt aatatcagta cttactgtct cctgagatag aagatctcca
     1441 acctttgcgg atatattaat ccttataaga tcggtttcta ttcttctatt ccctttaatg
     1501 tcaattctta ttatgttctc ttgacaaaat ccaattcggg tcaaagataa tgtaaaaaat
     1561 aatgtaatta gaatcctaaa gaaaaataca taagggcttt tagttctcat ctactatttt
     1621 tccatctgag agtctgattc ttctgccaag ccgcatagct atcattggat tatgtgtaac
     1681 caaagccaat gttatcccta cctcgacgtt aagctcaaga aatagatcca aaacagagtt
     1741 cccagtttct aaatccagat ttcccgttgg ttcatcagcc aaaatcacct ttggctttag
     1801 tacaacagcc cttgcaattg caaccctctg ttgttctcca cccgaaagtt ctccgggtct
     1861 atgtcttaat cgttcaccaa gccccaccct cctaaggatt attgctgcta tctcccttgc
     1921 ttcgtttagc tgaattcctt gtattaaaag tggcatcata acattctcaa gcgcgctaaa
     1981 ctccgagagc aaataatgga actgaaaaac aaatcctatc tcacggtttc taaaaagtgc
     2041 aagctcatca tcgtctctca tgaacacgtt ctcaccttga taaagaacct ctccgtcgct
     2101 aggacgttca agggttccga gtatgtgtaa tagtgtactt ttacctgatc ctgatacacc
     2161 tacaatacct accacttcac ctttattaat cacaaggttg agtcctttta acgcagtcac
     2221 tctgctgcca tcagtttcaa atacctttgt tagatccttt gcctttatta tctctttgct
     2281 cattcatacc tcaatgcttc cacagggtct tttcgcgacg cctgatatga tggatataag
     2341 gtcgctaaaa agcatatacc caaactcatg atcgccaccc ctaaaaagta aagcggttca
     2401 attttaactg gaaactcaga aacaggatag acctgtgtgt caaacgggat aagcttcttg
     2461 acagcatcac tagttttcac taaatagcaa attcctaatc ccgcgatact accaagaagt
     2521 gttccgacaa aaccaattat cattccatcc gttataaata ttctcataat tcctctacgg
     2581 gtggcgccca tagcacgcag tatcgcaata tcctttcctt tctccattac aaccatagtg
     2641 agtgtgctaa caatatcaag cgctgcaacg agtattataa gagcgaggaa gattgcaata
     2701 gcaatccttt caagacgaag cgctcgaaag aggcttctgt ttacctcctc ccagtttctt
     2761 gtataaaagg gaaagcctaa actcacggca atcttctcac cgatctccct cgctttatat
     2821 aagtctctaa ccttagcttc aacacctgta accttggagt tcatctcaaa aaaatccatc
     2881 gcatccctta aaccaacgta ggcgatagag gagtcatatt caaccattcc atagtcgaag
     2941 actccgacta cttcaaacct cttaattttc gcttgtggtc cgaatgggcc aacctttcca
     3001 aaacgagata ccaagtttac ttgatctccg gctgaaatcc ctaggtttga ggcaagttct
     3061 atacctaaga ttaaaggagg ctttcctgat ttagtttctc tattaagttt cgttatcagc
     3121 tctcgaccaa tttctctaag ttcatcggtc ttaacctttc cgttccttcc aaccatccct
     3181 cttcccaatg cctcctctat gtttgttacg ctgcccgcgt ttttagggtc gattccccta
     3241 atcaccgctc caagaacatt gttttcacct acaaccattc cctgtccgta tataaatggg
     3301 cttgtgccta ctaccccgtt tgtattaata atcttgtcct ttattctttc ataatctccc
     3361 ataggcccat cgtagctcag caccacaaga tgagagttca ccccaaggat tttatctctg
     3421 agttcctcct caaaacctct cattacagcc agaacaacgt ttagcgccat tacacccata
     3481 aacaccccaa gtatggatat taaccctatg acagaaataa aggtctgcct tctttttgat
//
LOCUS       DATDRL010000254         2790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2260764, whole genome shotgun sequence.
ACCESSION   DATDRL010000254 DATDRL010000000
VERSION     DATDRL010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2260764"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..523
                     /gene="ndk"
                     /locus_tag="VI935_08570"
     CDS             107..523
                     /gene="ndk"
                     /locus_tag="VI935_08570"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HLE25688.1"
                     /translation="MEKTLSLVKPDGVKKNLVGEVIKRFEQNGLRVIGVKMLWLSREE
                     AQGFYIVHKERPFFDSLTTFMSSGPIVALVLEGENAIKKTRELMGATNPKDAAKGTIR
                     GDFGGGIETNVVHGSDSKESADFEIPYFFSRMDLLI"
     gene            555..941
                     /gene="gcvH"
                     /locus_tag="VI935_08575"
     CDS             555..941
                     /gene="gcvH"
                     /locus_tag="VI935_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HLE25689.1"
                     /translation="MYPDNLKYHKDHMWLHSTGNKARIGITYFAQEQLGDIVFIELPA
                     VGTEVDANAIIGEIESTKTTSTLEAPVSGRIIEVNDNLEETPEIINDEPYKGGWIAVI
                     EMSDPKEVIDLMDSKTYQDLIEKESK"
     gene            982..2169
                     /gene="tyrS"
                     /locus_tag="VI935_08580"
     CDS             982..2169
                     /gene="tyrS"
                     /locus_tag="VI935_08580"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HLE25690.1"
                     /translation="MSNDKVQEHIRLIKRGSVEIISEEELKQKLERSVKENLPLKVKA
                     GFDPTSPDLHLGHTVLLYKLRQFQELGHKVFFLIGDFTGMIGDPSGVSETRRPLTRED
                     VLMNAATYQRQVFRILDPRYTEIVFNSQWMNSVTGEELIRLCGKYTVARMLEREDFKK
                     RYTTGRSIGIHEFIYPLIQGYDSLKLKADIELGGTDQKFNLLVGRDLQKEYGQEPQVV
                     ITMPLLEGTDGIKKMSKSLKNYIGIEEPPREIFGKVMSIDDQLMLRYYEILTSNNMDE
                     IKTMHPMEAKKRLAQELVERFHNREEAVKAREEFEKVFSHRKGPGADIPEYFIKDGKR
                     WLPHILTDAGITKSNSEAIRLIREGAVELDGEKVYDTNFELPQGGKRIVKVGKKRFIK
                     ISS"
     gene            2334..>2790
                     /locus_tag="VI935_08585"
     rRNA            2334..>2790
                     /locus_tag="VI935_08585"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          927 a          460 c          682 g          721 t
ORIGIN      
        1 aggattatta actaaaaatc tataaattgg ttgacaacca ttagatgttt acgatatctt
       61 tttgcaaata tatttttaca attatatctt aatgagggag tgctaaatgg aaaagacact
      121 ttcacttgta aaacctgatg gagttaaaaa gaatcttgta ggcgaagtca tcaaacggtt
      181 tgaacaaaac gggcttaggg taattggagt aaagatgcta tggctttcca gggaagaggc
      241 tcagggattt tatattgttc acaaggagag gccattcttt gatagcctta ctacttttat
      301 gtcgtcgggg ccaatagtgg ctttagtcct ggaaggggag aatgctatca agaagacaag
      361 ggaattgatg ggagcaacta atcctaaaga tgctgcgaaa gggactatca ggggagactt
      421 cggaggaggt atagagacta atgttgtcca tggttctgat tccaaagagt ctgcagactt
      481 tgagatccca tattttttca gtagaatgga tttattaata taaaaaactt aatataaact
      541 taggaggtag cttaatgtat cctgataatt taaaatatca caaagatcat atgtggttac
      601 attccaccgg aaataaggca agaatcggga ttacctattt tgcccaggaa caacttggtg
      661 atattgtttt tatagaacta ccggcggttg gaacagaggt ggatgccaat gctattattg
      721 gagagattga gtcaacaaag actacatcca ctcttgaagc ccctgtaagt ggcaggataa
      781 tagaggtaaa tgataacctt gaagagaccc ccgagataat taatgatgag ccttataaag
      841 gtgggtggat cgcagtaatt gagatgtctg acccgaaaga ggtaatagac ttgatggatt
      901 caaagaccta tcaggactta atagagaaag aatcaaagtg aattcatcag tcagaacagg
      961 ccagactata tatcaaataa catgagcaat gataaagtcc aggaacatat aaggctgatt
     1021 aagcgtggta gtgttgaaat aatatctgaa gaggaactta agcaaaaact tgaaaggtct
     1081 gtaaaagaga atcttccgct aaaagttaaa gccggttttg atccaacttc acctgacctc
     1141 cacctgggac atacagtttt attatataag ctaaggcaat tccaggagct ggggcacaag
     1201 gtatttttcc tgataggcga ttttacaggt atgataggtg acccgagcgg ggtttctgaa
     1261 acgagaaggc ctctgaccag agaagatgtt ttgatgaatg cagctactta tcaaagacag
     1321 gtgttcagga tactcgatcc aagatataca gaaattgtat ttaacagcca atggatgaac
     1381 agtgtaacag gtgaagagct tatccgcctt tgcggtaagt atacagttgc cagaatgtta
     1441 gaacgtgagg acttcaagaa acgatacaca accggccgtt caataggtat ccatgaattt
     1501 atttatccgt tgattcaggg ctatgactct ttaaagttga aggctgatat agaattaggg
     1561 ggaacagatc agaagtttaa ccttctcgtt ggaagagacc ttcagaaaga gtatggtcag
     1621 gaaccgcagg ttgtaattac catgccctta ctcgaaggta ctgatggtat aaagaaaatg
     1681 agtaaaagcc ttaagaatta tattggcatt gaagagccgc ccagggagat ttttggaaag
     1741 gtaatgtcta tagatgatca attaatgctg aggtattatg aaattcttac ttctaataat
     1801 atggatgaga taaagacgat gcatcctatg gaggctaaga aaaggcttgc acaagagctt
     1861 gtggaaagat tccataacag agaagaggct gttaaggccc gtgaggaatt tgagaaggtc
     1921 ttttctcaca gaaagggtcc cggtgcagat atacctgaat atttcattaa agatggaaag
     1981 agatggcttc cacatatcct taccgatgcc gggataacaa agagtaattc agaggctatc
     2041 agacttatta gagaaggggc agttgaatta gacggggaaa aggtatatga tacaaacttc
     2101 gaacttccgc aaggagggaa gcggatagta aaagtaggaa aaaaaagatt cataaaaatt
     2161 agttcttgac aagcccatat aattctcgta atatttaagt tcgttcaaaa atggcgtatc
     2221 aagagtaaca tttcttgaca aaaatatttg aatctgctat atttgatgtg cctaaaaaat
     2281 aggcacatgg tctttgaaaa ctaaatagcg tacacattga gcgggttaaa gtttttttgg
     2341 agagtttgat cctggctcag aacgaacgct ggcggcatgc ctaacacatg caagtcgaac
     2401 gagaaaaggg tagcaatacc ctgagtaaag tggcgcacgg gtgagtaatg catgggtaac
     2461 ctaccttgaa ggctggaata accctccgaa aggagagcta ataccggata acaagaagag
     2521 atgaaaatct ccttctttaa aggatccccc gcaaggggaa atcacttcaa gatgggctca
     2581 tgtcccatta gctagttggt agggtaatgg cctaccaagg ctgtgatggg tagccggtct
     2641 gagaggatga tcggccacac tggcactgaa acacgggcca gactcctacg ggaggcagca
     2701 gtgaggagtt ttgcgcaatg ggcgaaagcc tgacgcagca atgccgcgtg agggaagaag
     2761 gccttcgggt tgtaaacctc tttcgactgg
//
LOCUS       DATDRL010000255         8496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3245877, whole genome shotgun sequence.
ACCESSION   DATDRL010000255 DATDRL010000000
VERSION     DATDRL010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3245877"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /gene="dnaG"
                     /locus_tag="VI935_08590"
     CDS             complement(<1..1177)
                     /gene="dnaG"
                     /locus_tag="VI935_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HLE25691.1"
                     /translation="MNILNLIETYVSLKRSGRSYVGLCPFHEEKTPSFHVNEEKGVFH
                     CFGCGAGGDIFGFVMRYKNLTFAEAAAELSKQAGINIEKTNRDKVRKTRRDILFKLNR
                     LVSNYYHMLLLESEEGRAARDYLRKRGLTLDIAREFYLGYAPLGWDVLAKYLASKRVP
                     TSIAEDIGLIIKKKNKDEYYDRFRGRLMFPIRDVEGNALGFGGRTLTNEEPKYLNSPE
                     SEIYHKGRVLYGLDWARDYIRRKGEAVIVEGYMDFLSLYSVEIKNVVATLGTALTRDH
                     VVLLKRFTERPVVIFDGDEAGEKASLRVLDVFLEEGLSPHIVILPKGEDPASFISKEG
                     RDSLLRLIEGAVLLIDFYIERIQRDFLNRKISRNKAVDMVVEVLTKLKNPIERSHYIR
                     "
     gene            complement(1350..1541)
                     /gene="rpsU"
                     /locus_tag="VI935_08595"
     CDS             complement(1350..1541)
                     /gene="rpsU"
                     /locus_tag="VI935_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HLE25692.1"
                     /translation="MPGIVVGSNESIDSALRRFKKQVEKGGILSEVRKREFYEKPSER
                     KKKKLFASKKRIVRGTKRV"
     gene            complement(1646..1828)
                     /locus_tag="VI935_08600"
     CDS             complement(1646..1828)
                     /locus_tag="VI935_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25693.1"
                     /translation="MGLIDEIRKKRDSWMGRLEDHRQDEGEYEDDSMINSGNEEEEIL
                     RKRRMVGVFTGKERVK"
     gene            complement(2043..2903)
                     /locus_tag="VI935_08605"
     CDS             complement(2043..2903)
                     /locus_tag="VI935_08605"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HLE25694.1"
                     /translation="MHEGTSEKGISSSEGIKDNARKPIGNKEKIAKGKNTPKSVELAK
                     LPKEKPPIIESDRKIFEKEKPKVVIIVDDLGFMKEPIDKLLEIPAQLSFAVLPNLPYS
                     HYAAEMAYKNGRDVILHLPMEPTDSSGYAAFDAGEDVLLMGLPKDKILDKLEKNLTAI
                     PYIKGVNNHMGSKFTENTELMELILERIKSKGLFFVDSRTSKSTSGFKVAKELGIKTA
                     ERDLFLDEDSQRADYIKSQIERLIRVSKEKGYAIGICHPYPDTIKVLSEVIPDIKKEV
                     EIIPVSGVVN"
     gene            complement(3062..4207)
                     /locus_tag="VI935_08610"
     CDS             complement(3062..4207)
                     /locus_tag="VI935_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLE25695.1"
                     /translation="MKRRSIIVLLLFFVFAVSALGEEATYQGLSNFTRVLDLIERNYV
                     DNVDTEKLAISAIEGMLKTLDPYSAYLTPERYKELEIGTSGEFGGVGIEISGENDLLT
                     VITPIEGSPAAKAGIKPGDLIIAIDGKPTQGLSVDEAVKSLRGQKGSTVKITIQSQGD
                     KNPREVVLVRDIIFVKSVDSKLIDGRIGYIKLSQFQEKTSEELVKAIVALESENKSQL
                     NGLILDLRNNPGGLLTQAVEVADEFIDEGLIVSVKGRVEDQSTEYYATKEDNTPGYPI
                     VILVNKGSASASEVVAEALQDKKRAIILGTKTFGKGSVQSIIKLDDGSGLKLTTAKFY
                     APSGRSINQVGVIPDVIVENDENKDLQLERAKELLRSSKNVKTNPRG"
     gene            complement(4311..5312)
                     /locus_tag="VI935_08615"
     CDS             complement(4311..5312)
                     /locus_tag="VI935_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HLE25696.1"
                     /translation="MSRTQISNFLVDEVTEHVYIRETYGKTLVELGREYPNIVVLDAD
                     LTVSTKTNLFGKEFPNRFFTMGVSEQDMMATAAGFALSGKIPFASTFAIFATGRAWEH
                     VRQSIVFPKLNVKIVATHAGVTVGEDGATHQALEDIALMRVLPNMTVIVPADAVETEK
                     VIRTIVNHKGPTYVRLARAKFPTIFKKSYEFEIGKAKVLREGKHVTIFAIGIMVCEAL
                     EASKILEKEGISACVVNVSTVKPIDVETIVKTSNSTGAVVTAEDHNIIGGLGGAIAEV
                     LGENCPTPMKRIGMKDSFGTSGDGLQLLEYFGLKARNIVQAIHELLEFNSQKKSVVL"
     gene            complement(5309..6145)
                     /locus_tag="VI935_08620"
     CDS             complement(5309..6145)
                     /locus_tag="VI935_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HLE25697.1"
                     /translation="MHERTKNVDILKEIARRVRIDLLKALHRAKSGHTGGSLSSVEIL
                     VSLYFSELRHNPKNPRWEDRDRFVLSKGHGVPALYSVLAHAGYFAREELMSLRKPGSR
                     LQGHPEYDLEIGIEATTGSLGHGLSAANGMALSARMDGKDIRIYALLSDGELQEGSSW
                     EAITTSSYRKLDNLCAIVDRNRFQNDGAMKDIKDIEPVPDKWRAFGWEVETLPDGHDF
                     SSILSAFEKARHTKGKPFVIIANTIKGKGVSIFENQGKYHGVAPTDDELKVALNELGE
                     AA"
     gene            6437..6670
                     /locus_tag="VI935_08625"
     CDS             6437..6670
                     /locus_tag="VI935_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25698.1"
                     /translation="MTKMIIGVFERLFESYKVLVIQGSNGWAIPFRVLLIPISIFFYA
                     LTILVILAIVLLVILIAVPISTISKVRVSKARV"
     gene            6746..7495
                     /locus_tag="VI935_08630"
     CDS             6746..7495
                     /locus_tag="VI935_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HLE25699.1"
                     /translation="MKGKVESLLTQGKLPRHIVVIMDGNGRWANRKGLDRISGHKEGM
                     KSVKSVVKASRELGIKAITLYAFSVQNWNRPKSEVEALMELLKEYLIKEGNRLVEKEI
                     KLNAIGRLWELPSEVYEVLVKTMEKTKLCKKMTLTLALSYGGREEIIDAVKKIISDRI
                     SPKDLDEDSFSKFLYTSNLPEPDLLIRTSGEMRLSNFLLWQLAYTEIYVTKTLWPGFR
                     KRHLLMALLNYQNRERRFGLTSDQIRKREIS"
     gene            7492..>8496
                     /gene="dxr"
                     /locus_tag="VI935_08635"
     CDS             7492..>8496
                     /gene="dxr"
                     /locus_tag="VI935_08635"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HLE25700.1"
                     /translation="MKGISILGSTGSIGKQTLDITSRFPERFRIVGLAAGENISLLKE
                     QILNFKPEVVSIKDKDNARKLSKELKSESVEILWGEEGAQAVASHTRTGLVVSAMVGA
                     SGLKPTLSAIKSRKNVALANKEVLVMAGNILTEEALKNGINVIPVDSEHSALFQALRH
                     NGKEFVKRLILTASGGPFLNLPEENLADVTPEMALKHPTWKMGKKITIDSATLMNKAF
                     EIIEARWLFGIDPKSISVWIHPQSIVHSMVEFIDGSIIAQMSIPDMRIPISYAISYPD
                     RLPIDGNYNISRAFGELSFEDADLGKFPVLSLAYDALEEGGTMPAVMNAANEMAVQHF
                     L"
BASE COUNT         2426 a         1905 c         1481 g         2684 t
ORIGIN      
        1 ttcttatata atgacttctt tcaattgggt ttttgagctt tgtaagaact tcaaccacca
       61 tatcaaccgc cttgtttcta cttatttttc tgtttagaaa atctctttga attctctcta
      121 tatagaaatc aattaacaaa actgcacctt ctattaacct taataaacta tcccgtccct
      181 cttttgagat aaatgaagcg ggatcttccc ccttaggaag aataacaatg tgaggtgata
      241 acccttcttc gagaaaaaca tcaagaaccc taagagaagc cttttcgcca gcttcatccc
      301 catcgaaaat aacaactggc ctctctgtga aacgcttgag aagcactacg tggtcgcgtg
      361 tgagtgcggt gcctaaagta gcaactacat tttttatctc gacagagtaa agcgagagaa
      421 aatccatgta accttcaact atcacggcct ctcccttacg tctaatgtaa tcccgagccc
      481 aatcgagacc gtagagaaca cgacctttgt ggtaaatctc cgactcagga gagtttaagt
      541 acttaggctc ttcattggtt agagtccttc ccccgaatcc tagcgcatta ccctcgacgt
      601 ctcttattgg aaacattaaa cgtccccgaa acctatcgta atattcatct ttgttttttt
      661 tctttattat taacccgata tcctctgcta tactagttgg aacccgtttg ctggctaagt
      721 atttagcaag tacatcccaa ccaagggggg cgtatcccaa ataaaattcc ctagctatgt
      781 ctaaagtaag gcctcttttc ctcagataat ctcttgcagc cctcccctcc tcgctttcaa
      841 gaagaagcat gtgataataa tttgatacta gcctattaag tttaaagagt atatcccttc
      901 tggtttttct aaccttatct ctatttgtct tttcaatatt tattcctgct tgcttcgaaa
      961 gctccgctgc ggcctcagca aaggtgaggt ttttataacg cataacaaac ccaaaaatat
     1021 ctcctcccgc tccacatcca aaacaatgaa ataccccctt ttcctcattt acatggaaag
     1081 atggggtctt ttcctcgtga aaagggcaaa gccctacgta actccttccc gatctcttaa
     1141 gagatacata ggtttcgatg aggtttaaaa tattcaacct cccttttatc tctttaataa
     1201 cggtttcaga gacaaaatac cccatacggt aaagattgta taatattttt agtccgtatt
     1261 attaaaagaa gagaaagcga gttcaggaat atgaattcca aggtgttcca ccttccgggc
     1321 tgaatttgaa aaatatctaa actttttcct taaactctct tagtccctct tacgatgcgt
     1381 ttttttgatg caaatagttt tttcttcttt ctctcactag gcttctcgta aaactctctt
     1441 tttcttacct ccgacagaat gccccctttt tctacctgct ttttaaacct ccttaaggcg
     1501 ctatcaatgc tttcattact tccaactaca attcccggca ttcaatcccc ttctgctgct
     1561 aattgaaatt tcttacaccc taaaatatta aatcgagctt gtcaagtata tggttataga
     1621 gcggttataa attcacgtca tagtattatt ttaccctctc ttttccggta aacaccccta
     1681 ccattcttct ctttcgcaaa atctcctcct cttcatttcc actattgatc atagaatcat
     1741 cttcatactc tccttcatcc tgacggtgat cttcaagcct tcccatccat gaatcccttt
     1801 tcttcctgat ttcatcaata agccccatat tttggcctcc atagctaatt catcaattag
     1861 aatataccgc gtctattatg aaatatttag gaaataaaaa ccattcctga ccaagttaaa
     1921 agcatcagaa cattttctat aaattttaaa aatagagacc tgataaggtc aaaaattatt
     1981 agatcaatcg ctcaactaaa tttatgtaga agtcggtaac atataatagc taatttttaa
     2041 atttagttca ccacccccga aacaggtatt atctcaactt ccttttttat atccggaatg
     2101 acctcggaga gcactttaat tgtatctgga tagggatggc aaattcctat tgcataaccc
     2161 ttttctttag aaacccttat tagcctctcg atctgggatt tgatgtaatc agctctttgt
     2221 gagtcttcat cgagaaaaag gtctctttca gcagtcttaa ttcctaattc cttagcgacc
     2281 ttaaaaccac ttgttgattt agatgtccta ctatcaacaa aaaaaagccc cttgcttttt
     2341 attctctcaa gaattaactc catgagctca gtgttttcag taaactttga gcccatatgg
     2401 ttgttaacac ccttgatgta aggaatcgca gttagattct tctcaagctt gtcaagaatc
     2461 ttatccttag gtagtcccat aagaagcaca tcctcccctg catcaaacgc tgcatagccc
     2521 gaagaatctg taggttccat aggaagatga aggattacat cacgtccatt tttatatgcc
     2581 atctcagccg catagtgaga atagggaagg tttggaagga cagcaaagct aagttgagct
     2641 ggtatttcca gaagtttatc aattggttcc ttcataaaac ctaaatcatc tacgataata
     2701 acgaccttgg gtttttcctt ttcaaatatt tttctatcag actctataat gggtggcttt
     2761 tctttgggta attttgcaag ttctactgac ttaggagtgt ttttcccctt ggctatcttc
     2821 tccttgttgc caataggctt tctagcattg tctttgatcc cttctgaaga ggaaatccct
     2881 ttttcggaag ttccttcatg cacccatttc ttgatataga aaaatgcact taaacacagg
     2941 gcaaaaaata taataaatcc aaaagtaatt aagaagattt tccctaccct ttttgttcgt
     3001 cttttctccc ttgctttaag cttcctaaaa cctctttttc ttttccccag ggaatagctc
     3061 cttagcctct tggatttgtc ttaacgttct tagaagacct gagtagttct tttgccctct
     3121 cgagttgtag gtctttattc tcatcgtttt caacaatcac atctggaatt acccccacct
     3181 ggttaattga cctcccactc ggcgcataga actttgcggt tgtgagttta agccctgaac
     3241 catcgtcaag cttaataata ctctgtacgg aacccttgcc aaaggttttt gtgccaagaa
     3301 taatcgctcg tttcttatct tgcagtgctt ccgctacaac ctctgacgcg cttgcactcc
     3361 ctttgtttac gagaattaca ataggataac ctggggtgtt gtcttctttg gtagcataat
     3421 actctgtaga ttgatcttcc actcttccct tgacgctgac gataagaccc tcgtcaataa
     3481 actcatcagc cacctctacg gcttgagtga gtagaccgcc gggattattt cttaaatcaa
     3541 gtataagccc atttagttgt gatttattct cggattcaag tgctactatc gccttaacta
     3601 gttcctccga tgtcttttct tgaaactgag atagcttaat gtatcctatc cttccatcaa
     3661 ttaacttgga atcaacactt tttacgaaga tgatatctct gactagcacg acttctctcg
     3721 ggtttttatc gccctgactt tgtatagtta tctttactgt actacccttt tgacctcgta
     3781 gactctttac agcctcgtca acagaaagac cctgagtagg ttttccatca atagcaatta
     3841 taaggtctcc tggtttaatg ccagcctttg cagcaggtga gccttcaatt ggtgttatta
     3901 ctgtgagaag atcattttca cctgagattt cgatccctac ccctccgaat tcacccgatg
     3961 taccgatttc aagttctttg tagcgttcag gcgtaaggta tgccgaataa gggtcaagag
     4021 tctttaacat tccctctatt gcactgattg caagcttctc tgtatcaaca ttatctacgt
     4081 aatttctttc gatcaaatca agcactctcg taaaattaga aaggccttgg tatgtagcct
     4141 cctctcctaa ggcacttact gcaaaaacaa aaaaaagtaa taaaacaatt atagaccgtc
     4201 ttttcatatt aaatccctcc gcttaaaacc atgttcacac tcaaagtgat ttctaatctt
     4261 ggtgaggcta aataatcctc atgagttttt gaggtaaaaa ttcacaacta ttatagtact
     4321 actgatttct tttgagagtt aaactccaga agttcgtgta tcgcttgtac tatatttctc
     4381 gccttaagac cgaagtattc aagcagttgc aatccatccc cagaagtacc gaagctatct
     4441 ttcatcccta ttctcttcat cggcgtaggg cagttctcac caagcacctc agcaatggcg
     4501 cctcccaatc ctccgattat attatggtct tcagcagtta caactgcccc agtactattc
     4561 gacgtcttaa ctatagtttc aacatcaata ggttttacag ttgacacatt aaccacgcag
     4621 gcgctgattc cttctttctc tagaattttt gaagcctcaa gagcctcaca caccatgata
     4681 cctattgcaa agatagtcac atgtttcccc tctctaagaa ccttagcctt tccaatttca
     4741 aattcgtatg atttcttaaa aatcgttgga aactttgccc ttgcaagcct tacgtatgta
     4801 ggtcccttat ggttaacaat tgttctgatt accttctccg tctcaacagc atcagcaggc
     4861 acaataactg tcatgttcgg gaggactcgc ataagagcga tgtcttcaag cgcctgatgt
     4921 gtggctccat cttctcctac tgtaacccca gcatgagtag caactatttt tacatttagc
     4981 ttaggaaaca caattgactg ccttacatgc tcccatgctc ggcccgtagc aaatatcgca
     5041 aatgtgcttg caaaagggat tttaccagaa agcgcaaaac cagctgccgt tgccatcata
     5101 tcctgttctg aaacacccat tgtaaagaac ctatttggaa actccttccc gaaaagattt
     5161 gttttagttg aaaccgtaag gtcggcgtcg agaacaacta tattaggata ctctcttccc
     5221 agttccacaa gagtttttcc ataggtctct ctgatgtaaa catgctcggt cacctcatcc
     5281 actagaaaat tgcttatttg agtcctgctc atgcggcttc tcctaactca tttaaagcta
     5341 ctttgagctc gtcatcggta ggtgcgacac cgtgatactt gccctggttt tcgaatatcg
     5401 ataccccttt cccctttatt gtgtttgcaa tgatgacaaa tggttttccc tttgtatgtc
     5461 ttgccttttc gaatgctgaa agaatgctag aaaaatcatg cccatctgga agagtctcca
     5521 cctcccaccc aaaggcacgc cacttatcag gaactggttc tatatcttta atatccttca
     5581 tagcgccatc gttttgaaac ctgtttctat caacaatcgc acaaagattg tctagttttc
     5641 tataagaaga ggttgtaatc gcctcccacg aagacccctc ttgaagctca ccgtcactca
     5701 aaagtgcata tattctaata tcttttccat ccatcctagc tgaaagagcc attccattcg
     5761 ctgcggaaag accgtgccct aaggaaccag ttgtggcttc tatccctatt tctaggtcgt
     5821 attcgggatg cccctggagt cggcttcccg gctttcttag gctcataagc tcttcacgag
     5881 cgaaatatcc tgcatgtgca agaacgctgt aaagcgctgg aacgccatgc ccttttgata
     5941 aaacaaacct gtctctatcc tcccagcgcg ggtttttagg gttgtgtctc aactcggaaa
     6001 aatacaaaga tactaaaatt tctacagatg aaagcgaccc tccggtgtgt cctgatttag
     6061 cacgatgaag tgcctttaat aggtcaatgc gtacccttct tgcaatctct tttaggatat
     6121 cgacgttctt tgttctttca tgcatacttt ttgtatttat ttgtagtttt atgttttttt
     6181 atctataaca acaatggatc tttccagaat cattataata ttacctatcg ggttgtcaaa
     6241 ctgtgtgatt aatagtactt agccataagt ttttgcctga agatcattaa acaaagaatc
     6301 ctcagggaaa tgatacaagt taaacgtaga attgaattcc ttaggagggc tgagctcaaa
     6361 aggtacatta gtaaaatcct ctatccttga actggacttt gccattgtat ttctttaaaa
     6421 ttatagtgga aataaaatga cgaaaatgat aataggtgtt tttgaaaggt tatttgagag
     6481 ttacaaagtg cttgttattc aaggctcaaa tggctgggct attcccttta gggttttgct
     6541 catcccaatt tcaatctttt tctatgcgct caccattcta gttattttag caatagtcct
     6601 actagtaata cttatagcag ttccaatctc aacgatttcg aaagtaagag tctctaaagc
     6661 cagggtatga tcttacatta attagaattt atataatgcg tcccttagca tgaattgaat
     6721 tcatggtatt atattttact tatacatgaa aggaaaggtt gaatcacttc ttactcaagg
     6781 aaagctccca agacatattg tagtgataat ggacggaaac ggaaggtggg caaatagaaa
     6841 ggggcttgac cgaataagcg gccataaaga aggtatgaaa tccgtcaagt ccgtggtaaa
     6901 agcctcgagg gaattaggca taaaagcgat caccctctat gctttttctg ttcaaaactg
     6961 gaatagacca aaaagtgaag ttgaagccct tatggaacta ctaaaagaat acctgatcaa
     7021 ggaaggtaat cgtctcgttg aaaaggaaat caagttaaac gcaattggaa ggttgtggga
     7081 acttccttcc gaagtctacg aagtgctagt aaaaaccatg gaaaagacaa aattgtgtaa
     7141 aaaaatgaca cttacccttg ccctaagcta tggagggagg gaagagatta ttgacgctgt
     7201 aaagaagata atctctgacc gtatttcgcc taaagatctt gatgaggata gtttttctaa
     7261 gtttctatac acttctaatc ttccagagcc cgatcttctt atacgtacca gcggagagat
     7321 gaggctttct aattttctcc tctggcagct tgcatacacc gagatctatg ttacaaaaac
     7381 actctggccc ggttttagga aaaggcatct attaatggct cttctaaact accagaaccg
     7441 agaaagaagg tttgggctta caagcgacca aataagaaaa agggaaataa gttgaaagga
     7501 atatctattc ttggttcaac cggctcaatt ggcaaacaaa cgctcgatat tacatcccgt
     7561 tttcctgaga gatttcgtat cgtagggctt gctgcagggg aaaatatcag ccttcttaag
     7621 gaacagattc taaattttaa accagaggtc gtttctatta aagataaaga taacgctaga
     7681 aaactttcaa aagaattaaa atcagaatca gttgaaatcc tttggggtga agaaggcgca
     7741 caggctgttg cttcccacac tagaactggt ttagtagtat ctgccatggt cggagcatcg
     7801 ggtctcaaac ctaccctctc agctataaaa tcgagaaaga atgtggcact ggcaaacaaa
     7861 gaggtccttg ttatggcagg taatatatta acagaagaag ctttaaagaa tggaatcaat
     7921 gtaattcctg ttgacagcga acatagcgcc ctttttcaag cccttagaca taatggaaaa
     7981 gagtttgtca aaaggcttat tttaacagca tcaggcggtc ccttccttaa tctacccgaa
     8041 gaaaatctgg cagatgtaac ccctgaaatg gcactaaaac acccgacatg gaagatgggc
     8101 aagaaaataa caattgattc ggcaaccctt atgaataaag catttgagat aatcgaagca
     8161 agatggctct ttggtataga ccctaagagt atttcagtat ggattcatcc acagagtata
     8221 gttcattcta tggtagagtt tatagatggc tctatcatcg cacaaatgag cattcctgat
     8281 atgagaatac caatttcgta tgcaatctct taccctgata gactacctat agatggtaat
     8341 tataatatat ctagagcctt tggtgagctt tcttttgaag atgcagatct tggaaagttt
     8401 cctgtgcttt ccctcgctta tgacgctttg gaggaaggtg ggacaatgcc tgcagtaatg
     8461 aatgccgcaa atgaaatggc tgttcaacat tttctt
//
LOCUS       DATDRL010000256        15053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3247251, whole genome shotgun sequence.
ACCESSION   DATDRL010000256 DATDRL010000000
VERSION     DATDRL010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15053
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3247251"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..388
                     /gene="gcvH"
                     /locus_tag="VI935_08640"
     CDS             2..388
                     /gene="gcvH"
                     /locus_tag="VI935_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HLE25701.1"
                     /translation="MPEVLKYTKEHEWARVEDDIVIIGITDYAQDALGEIVYIELPSE
                     GDEVTKGETFGAVESTKSVSDLFAPISGEVVEVNEALLDSPEAINEDPYGEGWMVKVK
                     PYDPSELDDLMDSDEYTEFIEMQSEK"
     gene            563..3481
                     /gene="gcvP"
                     /locus_tag="VI935_08645"
     CDS             563..3481
                     /gene="gcvP"
                     /locus_tag="VI935_08645"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HLE25702.1"
                     /translation="MDDKVENDGLGKELFASRHIGPNETELEEMLKLIGADSLGSLIE
                     ETVPSAIRLKKPLDLPEAVSEYRFLEDISKIAKKNKVFKSYIGLGYSDCIIPSVIRRN
                     IFENPGWYTQYTPYQAEVSQGRLEALLNFQTMVCELTKMEIANASLLDEGTAAAEAMT
                     MLARLKSRDYHDSANIFFVSEKCFPQTIDVLKTRADPLGIKLTIGDHQSIEFKDKVFG
                     AIVQYPNGDGEVLDYSDFIYNAHNAGALVAVASDLLSLTLLTPPGEFGADVVVGSSQR
                     FGVPLGYGGPHAAFFATRDEFKRQVPGRIIGVSIDSHGNIAYRMALQTREQHIRREKA
                     TSNICTSESLLAIMASMYAVYHGPQGLRAIAERIHTLTRILDIELNKLAVKQDNSYFF
                     DTLRIDLGEGPADKLEKIKSLSNKAQVNFRYIDNRYIGISIDETTELEDIERIIQIFA
                     KASNQNYQKRYLRESARGIEIKFPKKLTRKSSYLIHPVFNKYHSETKMLRYIKSLEYK
                     DLALNTSMIPLGSCTMKLNATTEMIPVGWAEFSRLHPFAPREQAQAYAEILKELEGAL
                     CEITGFHACSLQPNSGAQGEYTGLMVIRAYHRDRDQGQRNVVLIPSSAHGTNPASAVM
                     AGMRVMVVKCDEEGSIDIEDLRSKALEHRDTLSTLMITYPSTHGVFEEKIQEICEIIH
                     EQGGQVYMDGANMNAQVGLSNPAIIGADVCHINLHKTFSIPHGGGGPGMGPICVAKHL
                     APYLPAHPLVDIGGKKSISPVASAPWGSASILLISYAYIKLLGWNGATDATRYAILNA
                     NYIKSRLEKHYPVLYTGSRGRVAHEFILDLRQFKRSSDIEVEDVAKRLMDYGFHAPTI
                     SWPVPGTMMIEPTESEAKEELDRFCDALISIRKEIQEIENGMADKNDNVLKNAPHTAM
                     ETASDTWKHPYSRLKAAYPIDFVKSNKFWPSVGRIDNAYGDKHLVCTCPSVESYTGET
                     "
     gene            complement(3537..5090)
                     /locus_tag="VI935_08650"
     CDS             complement(3537..5090)
                     /locus_tag="VI935_08650"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLE25703.1"
                     /translation="MKNINCKKMNREKIILSGAIKIAIFLITLTTILHAQEIKTLTYT
                     DGGIYEGETKNDKREGRGTMTWPDGTQYVGDFKDDMKSGQGTYTWPNGDKYEGEWGDD
                     KINGKGTLTYADGTKYVGDFIDGVKSGQGTYTWPNGDKYEGEWRDDERYGQGTMIWSD
                     GRKYVGEWKNDKRYGEGTLTFPNGDTYEGEFKDNTLDGKGTYTYADGRKYIGDFKDDK
                     FISGETIKPQVTENLIDKVAAVVNEDVITLSELKEIASTTNQNPEDTQVQSQVLELMI
                     EERLLQQEAKKLGIQVTEKEIDAAVEEVKRRYNLTEEQMDEALKKQNLTPESFREQWR
                     KQLLHNKLIGTQVQGQIAITEDEIKEYYEEKGGELESLEEIRIAHILITVESPDEEKQ
                     AENQALEIAKLAKSGEDFSELAKQFSKDTSSAERGGDLGYFKKGDMVVELEKAVIVTR
                     IGEIVGPVRSTAGYHVIKVIDKKMVEANSLDNAKEEIRERIYQEKVEESIKAWLENVK
                     KTAYIDKKI"
     gene            complement(5340..6227)
                     /locus_tag="VI935_08655"
     CDS             complement(5340..6227)
                     /locus_tag="VI935_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLE25704.1"
                     /translation="MKLISSLLLIALILGCSDFDNFYQGKRVGSKDLTGSSIVVAKIG
                     EETITKEDLIKSLNGLPPRQRVNYLSSPERLREYLDSFINQIVLFKEAEKMDIDKREK
                     IKESLQNFERRLLIQALSQDIMDHQISEEEIKKYYEENPNSFKRVKVSEIFIKANPEQ
                     GISKDEARTIAWLVVNRANAGESFEGLVKYFSDDITSKKRGGDFGYISRGSLPADIEN
                     KIFNLKIGEISDPIETENGFIIIKVTEDPKSIPLDQVIGEIQVELRKKTLSDYTKKLR
                     KQIGVEIFEDNLKKIGENG"
     gene            complement(6524..9988)
                     /gene="mfd"
                     /locus_tag="VI935_08660"
     CDS             complement(6524..9988)
                     /gene="mfd"
                     /locus_tag="VI935_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HLE25705.1"
                     /translation="MNEKKYIDQFTALHKFQHKNTFYNPILSSTLDQLLNKMVTGEKI
                     LKLSGLKVSSKFYIASTLAKKIERPILYLVSSKEIGKLAAKDMAFYLGEKPPILLKKE
                     LGLKNVLFSSQKDEYGERINWLYSASEKKPALSTSFSLRTSLSNGMLIAEAPALFQRT
                     IPIDVLRNSAIKIQKGSDIIRDNFISKLIQMGYLRSDFVEKVGDVSVRGAIIDLFSPG
                     FENPIRLELYGDAIHSIRFFRVDDQKSLETTEGAAILPVSEVILTDEAIKQSLSYVRK
                     KAEEQEIPASTKISLLEEIEKGTRFPEIEWFLPSFYQNLSTAFDYIPKDTLIIFDNPD
                     EISKSVKAYLSSLSDIKTSIKKHLKPTPEFQELYLTEDQLHDEILMFQRIFIEDVEIY
                     EAGIESIRFNTETANVQKEASFDSAFEFLSFKIQEWQALGIQVNLVLQTDIERKKIKD
                     ILTKRGIKNVNILIGSVSSGFIFPEGKIALITEKEISGERKQLKPTKSKDVPSVFITS
                     FSELKPGDFVVHVDFGIGIFRGLRRLKLENFEGDFLECEYEGGDKIYVPVDKLKLVQR
                     YIGDKKPRIDKLGHQNWKRVIKRVKKAVENVAKELLQLYAERKAEKGFQFSRRDSLFR
                     EFEFTFTYEETQDQEAAIYDVMSDMESPKAMDRLICGDVGFGKTEVAVRAAFKAVMDG
                     KQVAILVPTTLLAYQHYISFTERLKGYPVVTEMLSRFKTQKEIREIQKKLENGTIDII
                     IGTHKLLGNKVKFKDLGLVVIDEEHRFGVAHKEKLRKLKKGVEVLALSATPIPRTLQL
                     SLAGIRDISLINTPPEGRQSIENYVSQFNGDIIRDAIRRELNRSGSVFFIHNRIEDIL
                     RIAETIRELVPEAKIDITHGRMEEEKLEKAILRFIQGKTDVLVTTAIVEAGLDIPRAN
                     TIIINDAHAFGLADLYQLRGRVGRAEKKAYAYFLIPGVNILTPEAKRRLKAIAELREL
                     GSGFKLALSDLEIRGAGNIFGTEQSGHIAEVGLELYLEMLDKAIKELKKEKTHIEYEP
                     EVRLNTPAFLPDDYISDGAERLLFYKELSSVMSEKDIKEIKNELEDRFGELPEPALNL
                     VKVIELKLLLKKLLAQRIEIRNEETVIVFLEASPFYHHFTPSGRLKILYKHGRALEEI
                     KKRLKELK"
     gene            complement(10632..11711)
                     /gene="leuB"
                     /locus_tag="VI935_08665"
     CDS             complement(10632..11711)
                     /gene="leuB"
                     /locus_tag="VI935_08665"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HLE25706.1"
                     /translation="MLKILILPGDGIGVEVTNASVYVLKALGEKLNIKFEFESALFGG
                     SSIDAHGIPVTAEVLKKAKSSDAVLLGAVGGPKWDGLDYEIRPEKGLLTLRKELEAFA
                     NLRPAVVYPSLQSASTLKSEIVEGVDIMVVRELTGGIYFGTPRGITKLSNGEERGVNT
                     LSYTTTEIERVSRVAFQIARKRRKKLTSVDKANVLEVMELWRRTVTRIGKEEFPDVEL
                     EHLYVDNAAMQLIRRPRSFDVILADNMFGDILSDEAAQLTGSLGMLPSASLGNGRAIY
                     EPIHGSAPDIAGQDIANPIASILSSAMMLKYSFNMDNAAYEIDKAVRKVLEKGYRTRD
                     IYEKGTTKVGCQEMGRSILEEIRKQ"
     gene            12046..13857
                     /locus_tag="VI935_08670"
     CDS             12046..13857
                     /locus_tag="VI935_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE25707.1"
                     /translation="MSKRKKEHKEKKKKNQNVADSARFEKKGVSITSFLGNKGLVTLF
                     LFLLSFGAFAYSLKSDFVWDDLSRIKESSYRLNISDVNYKSLIPGLGKGIIGKHYRPL
                     LHISWIVDNEIWGVTPFGFHLSNIIFHSISTVLFYFMVLLILREFNAQRRESIAFLSS
                     IFFALHPIHVESVSFVAARADLLCSIFFFLAFIFNILSYRKLWFFILTIIFFYLSLLS
                     KEVAVAFPLVAIGFDLITGRFKSRGSFLRYPFYGLLIFGYFFFRSTLLESIIKLDLVY
                     VGGVLKVLFSSYFFYLEKLVFPFNLNPFISTRPSGSYYFLFSVLIIFILCVVAFVSIR
                     KREGITAFGIYWIFSTLGPHSLVAVLSVAITPLAERFLYIPSAGFCLLLGYFLVEAGK
                     RLNFKKLAWALGFILSLTYLFFTIKGQGVWKDNLTLWEYASKRSPINAIPHVNYGTSL
                     LQAGKTDEAIRELLFAFKPSVRSGKKQRIYASNNLGLVYLDKKDYESAEKWFNKTISD
                     DPRFDTAYYHLGVVYIIKGQNGNDASYYRKAEEYLKQAVKLRRSYGRAHLALANVYME
                     LGEREKARDEAKMALQSGLIESLANHARNILRKTENQ"
     gene            complement(13976..14734)
                     /locus_tag="VI935_08675"
     CDS             complement(13976..14734)
                     /locus_tag="VI935_08675"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLE25708.1"
                     /translation="MKFLRFKNKDGKTFAGVLEDSNVYEIEGDIFKEYKVTKNKHHLT
                     EIGFLPPCSPTKIVAVGLNYRDHAAEMKKPIPEEPLLFMKPSTSVIGHEDKIIYPKHM
                     SSRIDYEGELAVVIGKIARWVEENEAFDYVFGYTCINDITARDLQIKDVQYTRAKGFD
                     TFAPVGPVIETELDPSDLEISTFLNGERRQHSRTSELIFNVPRLIIFISRVMTLLPGD
                     IIATGTPSGIGSIKIGDKVEVKVEGIGILRNYVE"
     gene            complement(14873..>15053)
                     /locus_tag="VI935_08680"
     CDS             complement(14873..>15053)
                     /locus_tag="VI935_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLE25709.1"
                     /translation="RGPIAVRLAKTAINAGSEMDLKKALLLEKTLVSLCFDSEDRIEG
                     MKAFLDKREPSFKSK"
BASE COUNT         4077 a         3073 c         2865 g         5038 t
ORIGIN      
        1 gattccagaa gtgcttaaat acacaaaaga acacgagtgg gcaagggttg aggatgatat
       61 tgttataata ggtattactg attacgccca ggatgccctt ggggagattg tttacattga
      121 gcttcctagt gaaggagacg aggttacaaa gggagaaacc tttggtgcgg tggagtcaac
      181 taaatcggtc tcggatttat ttgcccctat aagcggcgag gtggtagagg ttaacgaggc
      241 tcttcttgat tccccagagg caattaacga agatccttat ggtgaggggt ggatggtaaa
      301 ggtaaaaccc tacgatccca gtgagcttga tgatctcatg gatagtgatg aatacactga
      361 attcattgag atgcaatcgg agaagtaatg ataaaaaagg atgattgatg agaggcttag
      421 attcataagc ttctaaagcg aatatttttt taaaagattt ttaaagatta cacctgttgt
      481 catcttttaa catcgccagg aaacttaatc attaaattgc atgcttgata gaggcccctt
      541 taattttaat agtaaaagga tcatggatga taaggttgaa aatgatgggt taggaaagga
      601 actgtttgct tcaagacaca tcggtccaaa tgaaaccgaa ctcgaagaga tgctaaagct
      661 aatcggcgct gattctttag gctcgctcat tgaagaaact gttccttcgg caattcgtct
      721 taaaaagccg ttagatcttc ctgaagcggt aagtgaatat cggttcttgg aagacattag
      781 caagatagcg aaaaaaaata aggtgtttaa gtcatatatt ggcctggggt attccgactg
      841 tatcatccct agcgtaatta ggcgaaatat atttgaaaac cctggttggt atacccagta
      901 cactccatat caagctgagg tttcccaggg tcgccttgag gcgttactaa actttcaaac
      961 tatggtctgc gagttaacta aaatggagat tgccaatgcc tctcttctag atgaaggaac
     1021 tgccgctgca gaggctatga ctatgctagc acgactaaag agtagggatt atcatgatag
     1081 cgccaatata ttctttgtct ctgaaaaatg ctttccacag accatagatg tattgaagac
     1141 aagagccgat ccgttaggaa ttaaactaac aatcggcgac catcagagta ttgagtttaa
     1201 agataaggtt tttggggcca tagttcagta tccaaatgga gatggcgagg ttcttgatta
     1261 ctctgatttt atttataatg cacacaatgc gggggcgttg gtagctgtcg cttcagactt
     1321 acttagtctt acattactta ctcccccagg cgaatttggc gccgatgttg tggtaggttc
     1381 aagccagcga tttggcgtgc cacttggata cggcgggcca cacgcagcct tctttgcaac
     1441 acgcgatgaa ttcaagcgtc aggttccggg acgaatcatt ggagtatcca tagattctca
     1501 tggcaatatt gcttatagga tggctctgca gactagagaa cagcatataa gaagagaaaa
     1561 ggctacttcc aatatctgca cttccgaatc tttgcttgcc atcatggcga gtatgtatgc
     1621 tgtatatcat gggcctcagg gccttagagc gattgcagag agaatccata ccctaacgag
     1681 aattttggat attgagctta ataagttagc ggttaagcaa gataattctt acttcttcga
     1741 tacattaagg attgacttag gagaggggcc tgcagacaaa cttgaaaaaa taaagtctct
     1801 ttcaaacaag gcacaggtaa attttcgata tatagacaat cgctatatcg gcatttcaat
     1861 agatgagacg acggaacttg aagatattga acgaatcata cagatctttg caaaagcaag
     1921 taaccagaac taccaaaagc gatatcttcg agagtcggca agaggcatag aaattaaatt
     1981 tcctaagaaa ttaacgcgta agagcagtta cttaatacat ccggttttta acaaatatca
     2041 ttccgagact aaaatgctac gttatataaa aagtctcgaa tacaaggacc tagctttaaa
     2101 tacatcgatg ataccgctgg gatcttgtac gatgaagctt aacgcaacaa ctgagatgat
     2161 ccctgtgggc tgggcggagt tttcaagatt acatcctttt gctccaagag aacaagctca
     2221 agcgtatgcc gagatattga aagaattaga aggagctctg tgtgaaatta caggctttca
     2281 tgcttgttcc ttacaaccta attcaggtgc acaaggagaa tacacgggtc tcatggttat
     2341 acgagcatat catagggata gggatcaggg acaaagaaat gtagtgttaa ttccatcttc
     2401 ggcacatggt accaatccag caagtgctgt gatggctggt atgagagtta tggtggtaaa
     2461 atgtgacgag gaaggcagta ttgacataga agacttgaga agcaaggcat tggaacacag
     2521 agatacttta tctacactta tgattactta cccatccacc catggtgtat tcgaggagaa
     2581 aattcaggag atttgtgaaa ttattcatga gcagggtggt caggtatata tggatggagc
     2641 caacatgaac gctcaagtcg gcctttcaaa tcctgccatt attggagcag atgtatgcca
     2701 tataaatttg cataaaacat ttagcattcc acatggggga gggggtccgg gaatgggtcc
     2761 tatatgcgtt gcaaagcatc tagcacctta tcttcctgca cacccattgg tagatattgg
     2821 gggtaaaaag tctatatcac cggtagcttc cgctccttgg ggaagcgcaa gtatcttatt
     2881 aatatcatat gcgtatatta agctactagg ttggaatgga gcaactgatg caacaaggta
     2941 tgcgattctt aatgctaatt acataaaatc ccgactagaa aagcactatc ctgttttgta
     3001 tacaggaagt cgtggaaggg tcgcacatga atttatttta gatctacgtc agtttaaaag
     3061 aagctcagat attgaggttg aggatgtagc aaagagactc atggattatg gtttccacgc
     3121 tccgactatc tcatggcctg ttccgggcac gatgatgata gaaccaaccg agagcgaagc
     3181 caaagaagaa ttagatcgct tttgtgatgc cctgatatct atacgaaagg agattcaaga
     3241 gattgagaat ggaatggcag ataaaaatga caatgtgtta aaaaacgcgc ctcacactgc
     3301 tatggaaacg gcctcggata cctggaaaca tccttattcc aggctaaaag ccgcttatcc
     3361 aattgatttt gttaaatcta acaaattttg gccatccgtt ggaaggatag acaacgctta
     3421 tggagataaa catctagtat gcacttgtcc ttcggtggag tcctacacag gagaaacgtg
     3481 aatagtggtt tgtgaatcgt gcatcgaacc acgagacacg aaacccgaac tacaatttaa
     3541 atcttcttat caatgtaggc tgttttcttt acattttcta gccacgcctt tatagattct
     3601 tcaaccttct cctgatagat cctttccctt atttcttctt tagcattgtc aagagaattt
     3661 gcctctacca ttttcttatc aataactttt atcacatgat accctgcagt ggaccttact
     3721 ggacctacaa tttctccaat ccgggttact ataactgctt tttcgagttc caccaccatg
     3781 tcaccctttt taaagtatcc tagatcacct ccccgttctg ctgatgaagt atctttagaa
     3841 aattgttttg caagctcact aaaatcctct cctgacttag ctagttttgc aatctctaga
     3901 gcttggtttt ccgcctgctt ttcttcatca ggtgattcaa cagtgattaa tatgtgcgca
     3961 atccttatct cttctaacga ttcgagctct ccaccctttt cctcgtaata ttcctttatc
     4021 tcatcttctg tgatagcgat ttgtccttga acttgagtac ctatgagctt attgtgtaga
     4081 agttgttttc gccattgttc tctaaaagac tcaggggtaa ggttttgttt ctttagcgcc
     4141 tcatccattt gttcttctgt taagttatat cttctcttta cctcttctac agcggcgtca
     4201 atctcctttt ctgtaacctg aattcctagt tttttagctt cttgctgtag aagtctttct
     4261 tctatcatca gttctaagac ctgactttgc acttgtgtgt cttctggatt ttggtttgtg
     4321 gtggaggcta tttcttttaa ttcagatagg gtaatgacat cttcgtttac aacagccgca
     4381 actttatcta tcaggttttc tgtaacttgg ggttttatgg tctctcctga aatgaattta
     4441 tcatctttaa aatcacctat atattttctc ccgtcagcat aagtatatgt ccccttgcca
     4501 tccagcgtat tatctttgaa ctctccttcg tacgtgtctc catttgggaa ggtcagtgtc
     4561 ccctcgccgt acctcttatc gtttttccat tctcctacgt attttctacc gtctgaccag
     4621 atcattgtac cctgaccgta tctttcgtca tccctccact cgccttcata tttatcaccg
     4681 tttggccatg tgtatgttcc ctgaccgctt ttcactccat ctataaaatc tcctacgtat
     4741 tttgttccat cagcataagt aagcgttcct ttcccattaa ttttgtcatc cccccactcg
     4801 ccttcatatt tatcaccgtt tggccatgtg tatgttccct gaccgctttt catgtcatct
     4861 ttaaaatcgc caacatactg tgtgccatcc ggccaagtca tcgtaccacg accctctctc
     4921 ttatcattct ttgtttctcc ttcgtatatt cctccgtctg tataagttaa tgtctttatt
     4981 tcctgagcat gtaaaattgt tgtaagagta atcaaaaata tggcaatctt aattgcacct
     5041 gacaatataa ttttctctct gttcattttt ttgcaattaa tgtttttcac ccttaacccc
     5101 cgaactttta actatattgg tctctctatt tatctaagcc gcccagcatt tctcgataag
     5161 agaataagat agaatcaaga actggataag aataagatat atatcctatt tttagactca
     5221 ttattgtcca ttatctgttt actttttcct gatatacttt tttaactcat attaatactt
     5281 tttgcatttg ccttggaaag tccagctaag actgagttgc tgaaatacaa tctttattat
     5341 tatccatttt cgccgatctt ctttaaatta tcttcaaaaa tttctacccc gatttgtttt
     5401 cgtagctttt tagtataatc tgacaatgtc ttttttctca actcgacttg aatttcccct
     5461 attacttgat ctaatgggat actctttggg tcttcagtta cctttattat aataaaccca
     5521 ttttcggtct caatcggatc gcttatttct cctatcttga ggttgaagat cttgttttca
     5581 atatcggctg gcaggcttcc tctcgaaata tatccgaaat cgcctcctcg cttttttgat
     5641 gtgatgtcat cggaaaaata ttttacaagc ccttcaaagc tctctcctgc attagccctg
     5701 tttacaacaa gccaagcaat ggttcttgct tcatcttttg agataccttg ctcagggttc
     5761 gctttaatga aaatctcgct tactttaact cgtttgaagc tatttggatt ttcttcatag
     5821 tattttttaa tttcttcttc acttatttga tggtccataa tatcctggct tagagcctga
     5881 atcaaaagcc gtctttcaaa attttgtagg ctttctttaa ttttttccct tttgtctatg
     5941 tccatttttt cagcctcttt aaatagaaca atttgattta tgaaggaatc taggtactcc
     6001 cttaatcttt caggtgatga aagataatta acccgctgcc ttggagggag accattaagt
     6061 gatttgatca aatcttcttt tgtgattgtc tcttcgccta tttttgcaac cactatggag
     6121 cttcctgtta aatctttaga tcctaccctt tttccctgat agaaattgtc gaaatcgcta
     6181 caacctagaa ttagtgctat taataataga gagctaatta atttcatttg ttatctccta
     6241 tagataagag ctataggtgt tagtaattgt aggccagaaa acgaaggatg tactttgtta
     6301 cagcactttt aacgccgcct actaataatg agagttgaga ctcttctcat tagaatataa
     6361 tttacatgaa aaaaatcatt gtgggtatga tatactcatc cggaacaatt taaaggttta
     6421 tggggagtgt ttgatcacga atagaagtta tggctgtcta tttcttaata atttagggta
     6481 atcggtttaa ccaaatcaaa aatgcttttg tttcatttta ttattatttc agttctttaa
     6541 gtcttttctt tatttcttca agagcccttc catgtttata aagaattttt aaccttccac
     6601 ttggtgtaaa atggtgataa aagggcgatg cttcaagaaa gactataacg gtttcttcat
     6661 ttcttatttc aatcctttga gcgagtagtt ttttgagtaa gagcttaagc tctataacct
     6721 tgacaaggtt taaagcgggc tcaggcagct ctccgaatct atcttctagt tcgtttttaa
     6781 tctctttgat atctttctct gacatgactg aagagagctc tttataaaaa agaagcctct
     6841 ccgctccatc gctaatataa tcatccggga gaaaagcagg cgtgtttaat cttacttctg
     6901 gttcgtattc tatgtgggtt ttctcctttt taagttcttt tattgcctta tcaagcatct
     6961 ctaaataaag ctcaaggcct acttctgcaa tgtgccctga ctgctcagtg ccgaagatgt
     7021 ttcctgctcc tcgaatttct agatcagaca aagcaagctt aaacccagag ccgagttccc
     7081 gaagctccgc gattgcctta agccttcttt tcgcttcagg tgtaagtata tttacaccag
     7141 gaataagaaa atacgcatag gcctttttct cagcccttcc cactctgcct ctaagctgat
     7201 aaaggtctgc aaggccaaag gcgtgtgcat cattaatgat tatcgtatta gccctcggta
     7261 tgtcgagtcc cgcctctact atagccgttg taactagcac atcggttttt ccttgaatga
     7321 acctaagaat agccttctct agtttttcct cctccatcct tccatgtgta atgtcaatct
     7381 tcgcctctgg cacgagttct cttatagttt ctgctattct aaggatatcc tcaatacggt
     7441 tatgtataaa aaacactgac ccgctgcgat taagttccct tctgattgca tcccgaatta
     7501 tatctccatt aaattgagaa acgtagtttt cgatagattg ccttccttca ggtggggtat
     7561 ttataaggct tatgtctcta atcccggcaa gagatagctg aagtgtgcgt ggtattgggg
     7621 ttgcactgag cgctaatacc tctacaccct ttttaagttt tctcaatttt tctttgtgcg
     7681 ctacgccgaa tctgtgttct tcatcgatta caacaagacc taggtcttta aacttaactt
     7741 tatttcctaa aagtttgtgg gttccaataa taatatctat cgtgccattt tcgagttttt
     7801 tttggatttc tcttatctcc ttttgcgttt tgaatcttga aagcatctca gttactactg
     7861 gatacccttt tagcctttcg gtaaatgata tatagtgctg ataagcaaga agtgttgtag
     7921 gtacaagaat agcaacttgt tttccgtcca taactgcttt aaatgctgcc cttactgcga
     7981 cctcagtttt tccaaagcct acatccccgc aaatcagtct atccatggct tttggtgatt
     8041 ccatgtcact catcacgtcg taaattgctg cttcttgatc ttgcgtctct tcgtaggtaa
     8101 aagtaaactc aaattctcta aacaagctgt cccttcttga aaactggaat cctttttctg
     8161 cttttctctc ggcgtagagt tggaggagtt ctttagccac attttcaacc gcctttttta
     8221 ccctttttat aacccttttc cagttttggt gccctagctt gtcgatcctt ggttttttat
     8281 ccccgatata tctctgaacg agcttgagtt tatcgacggg cacataaatt ttatcgccgc
     8341 cttcatattc acattcgagg aaatcccctt caaaattttc taacttgagc cttctaagcc
     8401 ctctaaatat ccctattccg aagtctacgt gaacaacaaa gtcgccgggt tttagctcac
     8461 taaatgatgt tataaagaca gatggtacgt ctttagattt tgttggcttt aattgtttcc
     8521 tttccccaga tatttccttc tctgtgatta atgcaatctt gccttcaggg aagataaaac
     8581 cagatgaaac cgatccgatg aggatattta cattttttat cccacgcttg gtgagtatat
     8641 ctttgatttt tttcctctcg atgtccgttt gcaataccag attgacttgt attccaaggg
     8701 cttgccattc ttgtatctta aaggaaagga actcaaaggc agaatcaaaa ctagcttctt
     8761 tttgaacgtt tgctgtttca gtattgaaac gtatgctttc gattccagct tcatagatct
     8821 ctacatcttc aataaagatt ctttggaaca ttaaaatttc atcatgtagt tgatcttcgg
     8881 ttagataaag ctcttggaac tcaggggtag gcttcaagtg cttctttatc gaagttttaa
     8941 tatcagataa tgagctcaaa taggctttaa cagattttga aatctcatcg gggttatcaa
     9001 atatgattaa agtgtcttta ggaatgtaat cgaatgcggt gcttagattc tgataaaatg
     9061 aaggtaggaa ccactcgatt tctgggaagc gtgtaccttt ttcaatctct tcaaggagtg
     9121 agattttggt tgaagcaggt atttcttgct cctctgcttt ttttcttaca tatgataagg
     9181 attgtttgat tgcttcatct gttaggatga cttcacttac cggaagaatg gcggcacctt
     9241 cggttgtttc taaggatttt tggtcatcta ccctgaaaaa tctgattgag tggattgcat
     9301 ctccatatag ttcaagtctt atggggtttt caaatcctgg cgaaaataga tcaataatgg
     9361 cacccctaac acttacatcc ccaacctttt caacaaagtc gctacgtaag taacccatct
     9421 gaatcagctt tgaaataaaa ttgtctctta tgatatcgct tcccttttga attttaatag
     9481 cagaatttcg tagaacatct attgggatcg ttctttgaaa aagcgcaggt gcttcagcta
     9541 taagcattcc attcgacaag cttgtcctga gtgaaaacga tgtgctcagg gcaggctttt
     9601 tctcgcttgc ggaatatagc cagtttatcc tttctccata ctcatccttt tgcgatgaga
     9661 ataaaacatt ctttaagccc aattcctttt ttaacaaaat gggcggtttt tctcctagat
     9721 aaaaagccat atcctttgcc gccaattttc cgatttcttt agaagaaacg agatataaga
     9781 ttggcctttc tatcttctta gctaaagtcg aggcgatata aaatttagaa gaaaccttta
     9841 aaccagagag ctttagtatt ttctcgcccg ttaccatttt attcagaagc tgatcaagag
     9901 tgctagaaag tattgggtta taaaaggtat ttttatgttg gaatttatgt agtgctgtaa
     9961 actgatctat gtattttttt tcattcataa tctgtcttca actccatagt tccctgtaac
    10021 ctgtgtcatt gcgagcgatg cggttcccta aatgccctcc ctctggaacg ctaccccaga
    10081 ccctgcgaag caatctcgtt ttttggattg cttcgtcgcc ttgctcctcg caatgacatt
    10141 cccgtgttgt ctgcgagtaa gctcctgata gatggggctt tctagtcccg tatcttgcag
    10201 ggttagaaaa ccccgcctat agtcaatcgc gagagccgaa ggtgtagcgt actgcttgtc
    10261 attgctgaac acattcgcgg agcttgtact gaggaaaccg aagtgctcag ggtaaactcc
    10321 gtcgaagagc tccctgcaat gatacaatga tatttcagtg ttacttcgag ctgaagtcga
    10381 agtgcctgtc tgagccctgt cgaaggactc gcaccaaagg gactatcatt tccataccga
    10441 tacactgcag cccgctgcag ggtcctttat taaaagaggc tagaaatcat aaatgtatct
    10501 cgtcactgtg tcatttgact ttttgatttt aggtgtgtga aggatacaaa gtatcatttc
    10561 ttttcattcc tcagggttat gtttcacgag ataatatttt agaaagttgt ccgttagcta
    10621 taaggttgac actactgttt cctaatttcc tctagaatcg atctacccat ctcttgacac
    10681 ccaacttttg ttgttccctt ctcgtaaata tctctagttc tgtaaccttt ttcaagaact
    10741 tttctaacag ctttgtcaat ttcgtacgct gcgttatcca tattaaatga gtatttaagc
    10801 atcatggcag aggaaagaat agaagcgata gggtttgcaa tatcctgccc agcaatatca
    10861 ggagcactgc catgaatagg ttcataaatc gctcttccat ttccaaggct cgcagaagga
    10921 agcataccga gggagcctgt aagctgtgca gcttcatcac ttagtatatc cccaaacatg
    10981 ttgtctgcta ggatcacatc aaagcttctg ggtcttcgaa tgagctgcat tgcagcgttg
    11041 tccacatata gatgctcaag ctctacatct ggaaactcct ctttaccgat tctagttact
    11101 gtccttctcc agagttccat aacttcgagc acgtttgctt tatcaacgga ggtaagcttc
    11161 tttcttcttt ttcttgcaat ctgaaaagcc acccttgaaa ccctttctat ctcggttgta
    11221 gtgtatgaga gggtgtttac tcccctttct tcaccgtttg aaagttttgt aatacctcga
    11281 ggagtgccga aatatatccc tcctgtaagc tctcttacta ccatgatgtc gacgccttct
    11341 acgatttcac tcttgagggt tgaggcgctt tgaagagatg gataaacaac agccggtctt
    11401 agatttgcaa acgcctctaa ttcttttcta agtgtgagaa gccccttttc tggtctaatt
    11461 tcataatcaa gaccatccca ctttggacca cctactgcac caaggagtac cgcgtcgctc
    11521 gactttgcct tctttaggac ttcagccgtt actggaatcc catgagcatc aatcgaagat
    11581 ccgccaaata gtgcgctttc aaattcaaat ttaatattaa gcttctcacc caaggctttg
    11641 agcacgtaaa cgctcgcatt tgttacttcc actcctattc catctccagg aaggataaga
    11701 atcttaagca ctttttttcc ccttagaata aatttataat taagatttat tttacctaat
    11761 gaactaatta tttaggaatt aattatgttt cttacaatta gaggtaatca tatattaaac
    11821 atagaaaagt tttgtattaa gcacaaaggt tattaacctt tttcctcgga actaatgggc
    11881 cgagaataga attttagaat aaaatcaaga tatatcatgt atgctacgct ttccaatgcg
    11941 gagtcatggc aattaaggaa aatcagctta taaaaatgta aataactgta ttgggttgaa
    12001 tcatgagcga aaggttgtca gggttttgtc tttcatttct aagagatgag taaaagaaaa
    12061 aaggaacaca aagaaaaaaa gaaaaaaaac caaaacgtgg cagattctgc ccgctttgag
    12121 aaaaaaggtg taagtatcac atcattcctt ggcaataagg ggttggtgac gcttttttta
    12181 tttctcctct cattcggggc gtttgcttat tcacttaaaa gcgactttgt ttgggacgac
    12241 ttatcgcgta taaaagagag ctcttataga ctcaacatat ctgatgtaaa ttataaatcg
    12301 ctaatccccg ggcttggtaa gggcattata ggaaagcatt atcgtccgct attgcatata
    12361 tcatggatcg tggacaatga aatatggggt gttactccct ttggttttca tctaagtaat
    12421 atcatctttc attccatctc gacagtgctg ttttacttca tggtccttct tatactcaga
    12481 gaatttaatg cgcaaagaag agaatctata gcttttctat catcaatttt ttttgcttta
    12541 caccccatac atgttgaatc ggtttcattc gttgccgcac gggcggattt gctctgtagt
    12601 atattctttt ttctagcgtt catatttaat atcctttcct acagaaaatt atggtttttt
    12661 atcttgacta tcattttctt ttatctttct cttctttcta aagaggttgc ggtagcattt
    12721 cctttagttg ctataggctt tgatctaatt actggtagat ttaaaagccg cggtagcttc
    12781 ttaagatacc ctttttatgg tttattgatt tttggttatt tcttcttcag gtcaacactt
    12841 cttgagtcta ttattaagtt agatttagtt tatgttgggg gggtgttgaa ggttttattt
    12901 agctcatatt ttttctattt agaaaagctt gttttcccct ttaatttaaa tccttttatt
    12961 tcaactcggc catcaggttc ttactatttt ctcttttctg tcctcataat ctttattctg
    13021 tgtgttgtcg cttttgtgtc tataagaaaa agagaaggaa taaccgcttt tggcatatat
    13081 tggatttttt ctactcttgg ccctcattct cttgttgctg tgctttcagt cgcaatcacg
    13141 cctcttgctg agagattttt gtatattcct tccgcgggat tctgcttact cttaggatat
    13201 tttctggttg aagctgggaa aagattaaac tttaaaaaat tagcttgggc tctaggattt
    13261 attctgtctt taacctattt attttttact ataaaaggtc agggggtatg gaaggataac
    13321 ctgacactat gggagtacgc ttccaaaagg tctcctatta atgctattcc tcatgttaat
    13381 tatgggacgt cgctattaca agcaggaaaa accgatgaag caattagaga actccttttt
    13441 gcttttaagc cgagtgtaag aagcggcaag aaacaaagga tttatgcgtc aaataatctt
    13501 gggcttgttt acttagataa aaaggactat gagagcgctg aaaagtggtt taataaaacc
    13561 atttctgacg atccaagatt tgatacagcc tattatcatc ttggggtagt ttatataatc
    13621 aaaggtcaaa acggaaacga tgcatcctat tataggaagg cagaggagta tttgaaacag
    13681 gcagtcaaat taagacgctc ctatggaaga gcgcatcttg ctttagctaa cgtttatatg
    13741 gaactcgggg aaagggaaaa agcaagggat gaggctaaaa tggcgttaca aagtggactt
    13801 atcgagtccc tagctaacca cgcccgtaat atactgagga aaaccgagaa tcagtaaata
    13861 agatttacct ggcgcttgcc atattctata caataacagg caatttattt tgaaatcgag
    13921 tcagtatcaa aagcgcagga tatatttaag agacatttga ctaaaatgac gctttttact
    13981 ctacatagtt ccgtaaaatt cctatgccct caacctttac ctctacttta tcgcctatct
    14041 taattgaacc tattcctgaa ggagtaccag ttgcaattat atctcctgga agaagcgtca
    14101 taacccttga gataaatatt atcagccttg gaacgttaaa aataagctcg gatgtacgag
    14161 aatgttgtct tctctcaccg ttaaggaacg ttgaaatttc aaggtcagat gggtcgagtt
    14221 ccgtctcaat gacaggtcca actggggcaa atgtgtcaaa ccccttcgcc cttgtgtatt
    14281 gtacatcttt tatctgaagg tcacgagccg taatatcatt gatgcatgtg tatccaaaga
    14341 cgtagtcaaa tgcctcgttt tcctctaccc atcttgcgat tttgccaatt actacagcaa
    14401 gctccccttc gtagtcgatt cgtgaagaca tatgtttagg atatattatt ttatcttcgt
    14461 gacctattac tgatgtgcta ggcttcataa aaagaagtgg ctcctcgggg atgggttttt
    14521 tcatctctgc tgcatggtct ctgtaattta accctacggc aacaatttta gtaggggagc
    14581 atgggggaag aaatccgatt tcagtcaggt gatgtttatt tttagtgact ttatattctt
    14641 taaaaatatc accttcaatc tcatagacgt tgctatcctc aagtacacct gcgaatgtct
    14701 taccgtcttt gtttttaaat ctaagaaatt tcattgctta cctttatttg tcattgcgag
    14761 ccgaaggcga agcaacccct cggttgttgt tggattgctt cgtcgcctcg ctcctcgcaa
    14821 tgacattctc ttttcacatt atggattgtt ttacgacata ctgtgataaa ccctacttac
    14881 tcttaaaaga tggttctctt ttgtcaagaa aggccttcat gccttctatt ctatcttcgc
    14941 tgtcgaagca cagggaaacc agggtttttt ctagaagtag tgccttcttt aagtccattt
    15001 ctgaacctgc attaattgcg gttttagcga gccttactgc tattggtccc ctc
//
LOCUS       DATDRL010000257         7275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_792380, whole genome shotgun sequence.
ACCESSION   DATDRL010000257 DATDRL010000000
VERSION     DATDRL010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792380"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..397)
                     /locus_tag="VI935_08685"
     CDS             complement(5..397)
                     /locus_tag="VI935_08685"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HLE25710.1"
                     /translation="MKKGAKKRAVTKKLPVRKTPKKPIGESGNLGIKKQYLKSGLSCR
                     VSFRLPKEAAIDAKKVTIVGDFNNWDSEATQMKRLKNGDFIVTLELNTGRAYNYRYLI
                     DGNRWENDWCADRYEQNPYGGENSVVEV"
     gene            complement(524..1060)
                     /gene="hpt"
                     /locus_tag="VI935_08690"
     CDS             complement(524..1060)
                     /gene="hpt"
                     /locus_tag="VI935_08690"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HLE25711.1"
                     /translation="MKEDIAKVLITEEDIRKRIKELGEEISRDYHGKKPVLVTVLRGA
                     IIFLCDLLREISIPVTLDFLSISSYTGQTHTGIVRILKDLDENIQNQHVILIEDIIDT
                     GLTLNYILRTLKERKPTDVRVCALLDKKVRRIVDIPIDYIGFEIPDEFVVGYGMDYKQ
                     NYRNLTFIGVLKEEILTK"
     gene            complement(1388..2299)
                     /locus_tag="VI935_08695"
     CDS             complement(1388..2299)
                     /locus_tag="VI935_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25712.1"
                     /translation="MAIITISELGDGLGQEVARKVSQRLGFILVDSTLIISKLGIPHK
                     IDVENDTEPLFQEKILPRLIKKLITEQALENNVVVLNLGGEILFRNLPGALHVKIHHS
                     FDRKKETKLIKHRKRSYLSFIKKLYAKKKIVGSFYDLQIKIENMDADFAVDMILKAVE
                     TRGIMAKAGVTWRGIEKLKASLNELGLYTRLNSEKVKKLGIPSFAHPSEKDFVRVLEF
                     YRIKWEYEPRSFPIEWDKDGKVIEEFTPDFYLPDLDTYIELTTLKQKLVTKKNRKLRK
                     LRELYPNVNIKIFYGRDYKKLLQRFGR"
     gene            complement(2523..3008)
                     /locus_tag="VI935_08700"
     CDS             complement(2523..3008)
                     /locus_tag="VI935_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HLE25713.1"
                     /translation="MEIKTVKIQKPEDINLILGQSHFIKTVEDLHEALFNTVPGIKFG
                     LAFCESSGPCLIRYSGTDPELVELARNNAFIVSAGHSFIIFLKNTYPINVLNAIKNVP
                     EVCRIFCATANAVEVIIAESEQGRGILGVIDGLKSKGIETEKDIEERRAFLRKIGYKI
                     G"
     gene            complement(3179..3340)
                     /locus_tag="VI935_08705"
     CDS             complement(3179..3340)
                     /locus_tag="VI935_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25714.1"
                     /translation="MSKFRYMMKNPTTNIRVLTFKQIDSSVIINSEATNLYYKDRLLH
                     GVYTELRMP"
     gene            complement(3378..5378)
                     /locus_tag="VI935_08710"
     CDS             complement(3378..5378)
                     /locus_tag="VI935_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HLE25715.1"
                     /translation="MSSKPEEKPKKLKDNPWIMRTYAGHTTAKASNELFRKNLAKGQT
                     GLSVAFDLPTQTGYDSDHPLAEGEVGKVGVPICHIEDIEALFDEIPIGKMNTSMTINA
                     TAAWLLSLYIALAERRGVDISLLEGTTQNDILKEYLSRGTHIFPPKPSMRLISDTITF
                     TSSKVPKWNPINICSYHLQEAGATPVQEIAFTLANAMAVLDAVRDSRQIPEDRFPQIV
                     GRISFFVNAGIRFIEETCKVRAFTEMWDELTLKRYGVKDDKYRRFRYGVQVNSLGLTA
                     QQPENNIARILLEMLSVTLSKNARARAIQLPAWNEALGLPRPWDQQWSLRFQQILGYE
                     TDLLEYPDIFDGSPVIERKVRELKKEAWKEIERIEEIGGALSAIEKGYMKMALVASHA
                     ERVRAIEKGEIQVVGVNCFTETEQSPLVQSVEKSIQKVDPKVVRQQLEKLKAYKVKRG
                     EKAVKEALRELSEAAKSDINIMPASIHCAHAGVTTGEWAGTLRRVFGEYRAPTGTSIT
                     LREDTNEIRTVRNEVKSLREKLGRPPKVLIGKPGLDGHSNGAEQIAVRARDVGMEVLY
                     QGIRLTPEEIVSTAEQENVDIIGLSILSGSHDTIIPKIMKLLNEKGLRDIPVVAGGII
                     PDDDAKALFETGVKRVYTPKDYSLNRIMRDFVEIIRESKDNV"
     gene            5788..6165
                     /locus_tag="VI935_08715"
     CDS             5788..6165
                     /locus_tag="VI935_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25716.1"
                     /translation="MGTSKIKVRVPPYRPPRYEWRKVIHQTVVRELKNGDLAYLPSDR
                     LEINVRLYLDKKSILHHDVDNRLKDMLDALQGRAGGSKKVHKLKPIIPNDNQVYKVSI
                     EKVLPPPQSHGLGHLVIKRYKQK"
     gene            6353..6613
                     /locus_tag="VI935_08720"
     CDS             6353..6613
                     /locus_tag="VI935_08720"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HLE25717.1"
                     /translation="MAIGKISEKGQLVIPKKLRIKYGLKAKSQVEWIDTGQGLVLIPI
                     PKDTIKSSRGMLRGTKITTETLLENRKRDKELERKKSDRVKR"
     gene            6610..7041
                     /locus_tag="VI935_08725"
     CDS             6610..7041
                     /locus_tag="VI935_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLE25718.1"
                     /translation="MTSPPSRFVLDSFAVIAFLRDEEGADKVEEFLKEADRGKTKLYM
                     HIINLGEVYYNLLKEEGENLANSVWAQVKNYPVEFLDDLSENFLLSAARIKGTYPLSY
                     ADAFAAAISIEKGALLITGDPDFKLLERDGKIKVLWIRDIK"
     gene            complement(7045..>7275)
                     /locus_tag="VI935_08730"
     CDS             complement(7045..>7275)
                     /locus_tag="VI935_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25719.1"
                     /translation="IEEAKHQLDFYHEMGGGSMFDPKTGQMIDEATAKIALMRVSKAY
                     MAGLVDKEYVDSMAEKSKSITGYDIRRAGERR"
BASE COUNT         2044 a         1526 c         1353 g         2352 t
ORIGIN      
        1 taggtcatac ctctactacc gaattctcac ctccgtaagg gttttgctca tatctgtctg
       61 cacaccagtc attctcccaa cgattaccat caattaggta cctgtaatta taggctcttc
      121 cagtatttaa ttctaaagtt acaatgaaat caccgttttt gagccttttc atttgtgtgg
      181 cctctgagtc ccagttatta aaatccccaa caattgtgac cttcttagca tctattgctg
      241 cctcttttgg cagtctgaaa ctaaccctgc acgaaaggcc agactttagg tattgttttt
      301 taatcccgag atttccagac tcaccaatag gtttctttgg cgtttttctc acaggcaatt
      361 tcttggtgac tgctcgcttt ttagcgccct ttttcatacc ggcctccata ggttttttag
      421 actgtataaa aaaattatat caaagaataa atgtgagttt tagaataatt attaagaagt
      481 ggacattggg aaaaaattca taaatttgaa ccaagaattt tcattattta gttaagattt
      541 cttccttaag aacgcctatg aatgtaagat tccgataatt ctgcttgtaa tccattccat
      601 accctactac aaactcatct ggaatttcaa accctatgta gtcaataggt atatccacaa
      661 tcctgcgcac ctttttatct aaaagagcgc atacgcgaac atcggtgggt tttctttcct
      721 ttagcgttct taatatatag ttaagagtca acccggtatc aattatgtcc tctattaaga
      781 ttacatgctg attctggata ttctcatcta gatccttcag gattcttact atgcctgtat
      841 gagtttgccc tgtataactt gaaatagaca aaaaatcaag ggtaacagga atagaaatct
      901 ctctcaacaa atcacataga aaaataatcg cccctcgcaa gacggtaaca agtacaggtt
      961 tcttcccatg ataatcacgc gatatctctt ctccaagttc cttaatccgc tttcttatat
     1021 cttcttctgt aattaaaacc tttgctatgt cttctttcac attaattcct ccaacagttt
     1081 tgaattgatt cctactctat aagtaaaatt aacaatatcg tctttaatct gccttaaatc
     1141 gttgtaactt attaaaaata aacgattatt ttgcttataa aaatttcact tgacattagc
     1201 ttcatcatgt agtatatata atattaacgg ttccaagagg ctcagggtta tttgaaaacc
     1261 aaagcccaaa gtaccaaaag aagaccgaaa ggtcaagtag gaacgcccct cgacgaggga
     1321 gaggctagtt aaaagctaag ccaggcaagg ttttcaaaag taaaaggtca cttggagccg
     1381 tttcatatta tctaccgaat ctctgtagta atttcttata atccctccca taaaatattt
     1441 ttatgttgac gtttggataa agctctctta gcttcctgag cttacgattt ttctttgtca
     1501 caagcttttg tttaagagtg gtaagctcta tgtaagtgtc tagatcaggg aggtaaaaat
     1561 ctggggtgaa ttcttctatt accttgccgt ccttatccca ttcaatagga aaactcctag
     1621 gttcatattc ccacttgatt cggtaaaatt ccaaaacacg aacaaaatcc ttttcactag
     1681 ggtgagcaaa ggaaggaatt cctaattttt taaccttctc tgagttcaat ctagtatata
     1741 aacctagctc attcaagctc gcttttaatt tttcaattcc tctccaagtc actccagcct
     1801 tcgccattat gccccttgtt tcaactgctt ttaatatcat atccaccgca aaatccgcat
     1861 ccatattttc tattttaatc tgaaggtcat aaaaactacc tactattttt ttctttgcat
     1921 aaagtttctt gataaagctt aagtaacttc tttttctgtg tttaattaat ttagtttcct
     1981 tttttcggtc gaaagaatga tggattttta catgaagggc gccaggaagg ttgcgaaata
     2041 gaatctcgcc tccaaggtta agtactacta cgttattttc gagagcttgt tcggttatta
     2101 gctttttaat aagtcttggc aggatttttt cttgaaagag gggctcagta tcattttcta
     2161 cgtctatctt gtgaggaata cctaacttgc taataatgag ggtgctatct actaaaatga
     2221 atccaagcct ttgagaaacc tttctagcaa cctcttgacc tagaccatca cctaattctg
     2281 atatagtgat aatagccatc tatttttaat tggatattat aattgtattt tctattatga
     2341 atgttaacta agtaaattct ggttggacat ttagagttca acaggattaa agttcatacc
     2401 ccccttacac attctcaata gcaaccccaa aaactgtagg agcggcttct agccgcgatt
     2461 cagtcgcgcc atggatggcg ctcctacaag atccgaaagt ttgtcttatt ctttttctcc
     2521 gctcatccaa tcttgtatcc aatttttctt aaaaaagccc ttctctcttc gatgtccttc
     2581 tccgtttcaa tcccttttga ttttaaacca tcaataacgc cgaggattcc tcttccttgt
     2641 tctgactcag cgataatcac ttcaacagcg tttgctgtag cacaaaatat tctacaaacc
     2701 tcaggaacat tttttatagc gttaagcaca ttgatcggat aggtgttttt aagaaatatt
     2761 ataaagctat gccccgctga aacaataaat gcattatttc ttgctaattc tacaagctca
     2821 gggtcagtac cgctatatct tataaggcag ggacccgatg attcacaaaa ggcaagtcca
     2881 aactttatgc cagggactgt attgaacaaa gcttcgtgaa ggtcttcaac agttttaata
     2941 aaatgtgatt gacctaaaat aaggtttata tcttcaggct tctggatttt tacagtttta
     3001 atttccatgg ctttctacgt tatatatgtc aataattctc ttgtcattcc gagtcaaccg
     3061 aaataatctc attattagat tgctttgctc ctttcagtcg ctcgcaatga cggctttttc
     3121 tgccgttgag aggtttcttt tgcctgtcgt tgcggacaca ctcatccttc ggtttcactc
     3181 agggcattct tagttcagtg taaactccgt gaagcaatct atccttatag tatagatttg
     3241 ttgcctcgct gtttataatc accgaagagt ctatctgttt aaatgtcaac acccttatgt
     3301 ttgttgtagg gttcttcatc atgtatctaa acttgctcat tatttcgtat tacctaaatt
     3361 ctatgaggga tcaaaaatca aacattatct tttgattctc tgattatctc aacaaaatct
     3421 ctcataattc gattaagcga ataatcctta ggggtgtaaa cgcgctttac tcctgtctca
     3481 aagagggctt ttgcatcgtc gtctggaatg attccacccg ctacaacagg gatgtctcgg
     3541 agcccctttt cattaagaag tttcataatc tttggtataa tcgtgtcatg tgagccagag
     3601 agaatactta gtcctattat gtcaacattc tcctgctctg cggttgagac aatctcttca
     3661 ggtgttaacc gaattccttg ataaagcacc tccataccta catcccttgc acgaaccgca
     3721 atctgctcag ctccattgga atgtccatca aggccgggtt ttccaattag cacctttggt
     3781 ggccggccca gtttttctct aagtgatttt acttcgtttc ttaccgttcg gatttcgttc
     3841 gtatcttccc taagtgttat tgaggttcct gtaggggctc tgtattcacc aaagacgcgt
     3901 cggagcgtac ccgcccattc gcctgttgta actcctgcat gagcacagtg tattgatgca
     3961 ggcataatgt ttatgtcact cttagcagct tcactaagtt cacgaagggc ctctttgact
     4021 gctttctcac ctcttttgac tttatatgct ttaagttttt ctaattgttg tctcacaacc
     4081 ttggggtcaa ccttctgtat tgatttttca actgactgta caaggggcga ttgttctgtt
     4141 tcggtaaagc agttaactcc aacgacttgt atctcccctt tctcgattgc acggacgcgc
     4201 tcggcatgtg acgcaacaag tgccatcttc atatatccct tttcaattgc tgaaagcgct
     4261 ccgcctattt cttctatcct ttcgatctct ttccaagcct cttttttaag ctctctaacc
     4321 ttcctttcaa tcacaggtga cccatcaaat atatctgggt attcgagtaa atctgtttca
     4381 tagcctagaa tctgctggaa tctaagtgac cactgctgat cccaagggcg cggaaggccg
     4441 agtgcttcgt tccaagcagg aagctgaatt gcccttgcac gtgcattttt gcttaatgtt
     4501 accgaaagca tctcaagtag aattcttgcg atgttatttt caggttgctg tgctgtaaga
     4561 ccgagggaat ttacctgaac gccatacctg aagcgacggt atttatcgtc ctttactccg
     4621 tatcgcttga gggtgagttc atcccacatt tccgtgaatg ccctcacctt gcacgtctct
     4681 tcgatgaagc gaatcccggc gttaacaaag aacgaaattc gtcctactat ttgagggaat
     4741 ctatcttcag gaatctgtcg gctatcacga acggcatcga gaacagccat tgcgtttgct
     4801 agtgtgaaag cgatctcttg cacaggtgta gctcccgctt cttgaagatg gtagctgcag
     4861 atattaatcg gattccattt tggaactttt gaagatgtaa aggtgatagt atccgagatt
     4921 aggcgcatag atggttttgg aggaaagata tgagttccgc gggaaagata ttctttcagt
     4981 atgtcattct gagtggttcc ctcaagtagt gaaatatcca caccgcgcct ttcagcaagc
     5041 gctatgtaga gagaaagaag ccatgctgca gtggcattaa tcgtcatgga cgtattcatt
     5101 ttccctatag ggatctcgtc aaaaagcgct tcgatgtctt caatgtgaca aattggaacg
     5161 cctacctttc cgacttcacc ctcggcaaga ggatgatcag agtcgtatcc tgtttgagta
     5221 ggtaggtcaa aagcgacgct aagccctgtc tgtccctttg caaggttttt cctaaatagc
     5281 tcattcgaag cctttgcagt cgtatgccct gcataagtcc tcataatcca ggggttgtct
     5341 tttaactttt tcggcttttc ctcaggtttt gaagacatag atttgtctcc ttatttgaat
     5401 taacgcaaag gtctttcaat cactaatgga gtaacagata gagggtaggt aacgagtccc
     5461 ctcgtcttaa gctcgggaca gacttgctcg ttactatcca ccggcaagcc tgtcctgagc
     5521 gaagtcgaag gaactggcgg gctacccgca agctaaactg gtatttgtta ctcgcttagg
     5581 ttttcaaaga ccctaaagct aattttatct gaaaagtggg agaagggaaa tgtgttcgta
     5641 accctttggt taacttctgg agacatttac tagagtgtct ccaagataac aaatattgcg
     5701 aatatttcct tgtaacagcc gatcaggatg attatacgaa atatttctct tataattagt
     5761 agttagttaa aatttaatag aatgagaatg ggaacatcaa aaataaaggt cagagttcct
     5821 ccatatcgcc caccgagata tgaatggcgc aaagtaattc atcaaactgt agttcgtgag
     5881 ttgaaaaatg gcgacctagc ttatttgcct tcagatcgtt tagaaattaa tgttcgactt
     5941 taccttgata aaaagtcaat cttgcatcat gacgtagata atagattaaa agatatgctc
     6001 gatgcattac aagggcgagc aggtggctcc aaaaaggtgc ataaactcaa accaataatt
     6061 ccaaatgata atcaagttta caaagtatcg attgaaaagg ttttgccacc tccccagagt
     6121 catgggttag gtcacttagt tataaaaagg tataaacaga agtaggagag taactccttt
     6181 atcacaatgt atcctacgag gtcattcgcc agcttcttat aaaccaaagc atgtgcttca
     6241 gtttatatct agttcatatc aaggattgtc tttagctgcg aggaaattgt ttacttttca
     6301 tataattgtt ttacaatatt atataaagta aactatataa aggagaagta agatggcaat
     6361 aggaaaaatt tctgaaaagg gtcagttagt tataccgaaa aagcttagaa taaagtatgg
     6421 acttaaggct aagtcccaag tcgagtggat tgatacagga caagggttgg ttcttattcc
     6481 tatacctaaa gacacaataa aatcctcacg tggaatgctt aggggtacaa agatcacaac
     6541 agaaacactg cttgagaata gaaaaaggga taaagaatta gaacgtaaaa aatctgatag
     6601 ggttaaaaga tgacctcccc tccatctaga ttcgttctgg attcttttgc ggtaattgct
     6661 tttcttcgag acgaggaagg agcggataaa gtagaagagt ttctcaaaga agcagataga
     6721 ggaaaaacta aactctacat gcacattatc aacttaggag aggtatacta taatttgttg
     6781 aaagaggaag gggagaatct ggctaacagt gtttgggcac aagtaaagaa ctatccagta
     6841 gagtttttag atgatctatc cgagaacttt cttctgagtg ctgcacgcat caagggaaca
     6901 tacccacttt cctatgctga tgcttttgct gctgcgatat ctattgaaaa aggagccttg
     6961 cttataacag gagacccgga ttttaagctc cttgaaaggg atggaaagat taaagtactc
     7021 tggattaggg atattaaatg atttttaccg tctctcccct gcacggcgta tgtcatatcc
     7081 ggtgatggat ttactctttt ctgccattga atcaacgtat tctttgtcaa ctaagccagc
     7141 catgtaggct ttggataccc tcataagagc tatctttgct gtggcttcgt caatcatctg
     7201 tccagtctta gggtcaaaca tcgagcctcc gcccatctca tgatagaaat caagctggtg
     7261 ctttgcctct tcgat
//
LOCUS       DATDRL010000258         2503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1139479, whole genome shotgun sequence.
ACCESSION   DATDRL010000258 DATDRL010000000
VERSION     DATDRL010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139479"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VI935_08735"
     CDS             <1..1050
                     /locus_tag="VI935_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLE25720.1"
                     /translation="WGVYQPLITFIAGIATAIFLWVGGRSTITLGITLGDFAAISIYL
                     GMLTWPMVAMGWAVDIIKRGNASIKRVNEILLTRPEIADSPDSVDIEIKGKIEFRDLS
                     FTYPNGKKALTNINLTIPEGTSLGITGGTASGKSTLLKLLMKIIELSEGQIFTDGIDL
                     RKIKKDSLRKGLIFLPQETVIFSGTIRDNIAFMNPDVSDEGIIEAAKLAEIYNEIESF
                     PGGLDARVGERGLSLSGGQRQRIALARAILLKPKVLILDDVLSSLDLRTEGLVLRNLK
                     RFMNGKTIIVVSSKVPSISGFDKLAVLEKGKLIELGTHDDLMNRDGIYTRLYKVQTLD
                     RSVTTQPQVYINAHE"
     gene            1205..>2503
                     /locus_tag="VI935_08740"
     CDS             1205..>2503
                     /locus_tag="VI935_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLE25721.1"
                     /translation="MDQGEEKGLKISYEIKLLKWLLNFASPYKRFMALSLIFMIITAI
                     FELIVPYVTKTAVDKYIFPSWREARFLDVREPLDSAQDRPQSNDENKDKGFENQLKEK
                     YPSAIITLDKDNYLIDLSRVSKEDRITLEKLGIVSEERYLVINLEDIEISKRERVLSI
                     IKNRPDIFKSIGGFFVSNYSSLKKLSSEDIRILRDQEINHLDRLALFLFLMLSAIFFF
                     SSLYTYLLHYSGQRIMHRIRVSVFSHLLTLPQPFFDKNPVGRLTTRVTNDVNAINEMY
                     TSVLVQFFKDILVIFGVLFVMFNMNRNLTFFIFILTLVLGVIALLFRNRLKSVYRNVR
                     KSIAKLNAFVQESIKGIMIIRLYNRETENFVKFRDVNRENYKANMDQLFAFATFRPII
                     EFISTTAVALILWYGGLSILNLDLTLGALIAYLAYIRMLFG"
BASE COUNT          777 a          419 c          529 g          778 t
ORIGIN      
        1 tggggtgtct atcaaccact tattacattt atagcaggga ttgcaacggc aatatttcta
       61 tgggtaggag gaaggagcac gataaccctt ggtataactc tgggtgattt tgcagctatt
      121 tcaatatatc ttgggatgct aacatggcct atggtagcca tgggttgggc ggttgatatt
      181 attaaacgtg gtaacgcatc aattaagcgt gtaaatgaaa tactgcttac aagacccgaa
      241 atcgctgatt ctccagattc agttgatatt gagataaaag ggaaaatcga gttcagggat
      301 ctcagcttta cttatcctaa tggaaagaag gccttaacta atattaactt gactatccct
      361 gaaggaacgt ctctcggtat tactggaggc acagcttccg ggaagagcac ccttcttaag
      421 cttcttatga aaataataga gctatctgag ggtcaaatat ttacagatgg gattgatcta
      481 cggaaaataa aaaaagatag cttaagaaaa ggactcattt tccttcctca agaaaccgta
      541 atctttagcg gaacgataag agataacatt gcttttatga atccagatgt ttctgatgaa
      601 gggataatag aggctgctaa acttgctgag atctacaacg agatagaaag ttttcctgga
      661 ggtcttgatg cacgagttgg ggagagagga cttagtcttt caggtggaca gaggcagaga
      721 attgcacttg caagggcgat tctattaaaa ccaaaggtct tgatacttga tgatgtcctt
      781 tcttctcttg atttaagaac cgagggttta gtgcttagga atcttaagcg gtttatgaat
      841 ggtaaaacca tcatcgttgt gtcaagcaag gtcccttcaa taagtggttt tgataagtta
      901 gcagttttgg aaaaggggaa attgattgag cttggaactc atgatgatct tatgaatcgg
      961 gatgggattt acacaaggct atataaggtg cagacacttg accggagcgt aactactcag
     1021 ccacaagtct acataaatgc acacgaatga aaataactat gatacaggat gcaagatgca
     1081 ggatacagga tacaagaaaa gcacgtcatg aatcatgcat cgtgtatctt gcatcactcc
     1141 atattagcgt gaatttgtgt atgttagtga ttaataatca tttggagaac ccgctttgag
     1201 ctcaatggat caaggagagg aaaaggggtt aaaaattagc tacgagatta agctattaaa
     1261 gtggctcctt aattttgcat ccccatataa aaggtttatg gcgctttccc taattttcat
     1321 gattatcaca gctatcttcg agcttatagt tccttatgtt acaaagacag ccgtagataa
     1381 gtacatattt ccctcttgga gagaagcgag gtttttagac gttcgtgagc ccttagactc
     1441 tgctcaggac aggcctcagt cgaacgatga aaataaagac aagggatttg aaaatcagct
     1501 caaagaaaaa tacccatcag caataattac tctcgataaa gataattact taattgatct
     1561 ttccagggtt tcaaaagaag acaggattac gcttgaaaag cttgggattg tctcagaaga
     1621 aagatattta gtcattaatc ttgaagatat tgaaataagt aagagggaaa gagttctcag
     1681 tataattaaa aatcgtcccg atatttttaa atcaattggt ggtttttttg tttcgaatta
     1741 ctcttctctt aaaaaactaa gtagtgaaga cattagaatc ctacgagatc aggagattaa
     1801 tcaccttgat agactagctc tttttttatt tttaatgctt tctgccattt tcttttttag
     1861 ctctctttac acctaccttc ttcattactc aggacaaagg attatgcata gaattagggt
     1921 aagtgtgttc tctcacttgc tgactcttcc gcaaccgttc ttcgataaaa accctgttgg
     1981 gagattaaca acacgggtga caaacgatgt gaatgcaata aacgagatgt acacctcggt
     2041 actggtccag ttttttaaag atattcttgt gatttttggg gttctatttg tgatgtttaa
     2101 tatgaataga aatcttactt tcttcatctt tattctcact ctagttcttg gggttattgc
     2161 tttacttttt agaaataggc tcaagtcggt ttatagaaac gttagaaaat caatcgcaaa
     2221 actaaatgcc ttcgtacagg aaagcataaa agggattatg attataaggc tctataatag
     2281 ggagactgaa aattttgtaa agtttaggga tgttaaccga gaaaactata aagcaaatat
     2341 ggatcagctt tttgcatttg ccacgtttag acccatcatt gagtttatta gtaccactgc
     2401 cgtggctctt atactctggt atggggggtt gagtattctc aatcttgatc tcacgcttgg
     2461 tgctcttatt gcttatcttg catacataag aatgctcttt gga
//
LOCUS       DATDRL010000259         4432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3561159, whole genome shotgun sequence.
ACCESSION   DATDRL010000259 DATDRL010000000
VERSION     DATDRL010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3561159"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VI935_08745"
     CDS             <1..277
                     /locus_tag="VI935_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013304288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HLE25722.1"
                     /translation="GGGVPRNWAQQVTPMIEFIKHGVQILEDREIGYNYGVLITTDQP
                     QFGGLSGSTLEEAISWNKYAPQARFATVHCDMTIALPIMVGAVKEWL"
     gene            complement(607..1596)
                     /locus_tag="VI935_08750"
     CDS             complement(607..1596)
                     /locus_tag="VI935_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HLE25723.1"
                     /translation="MRFKLFSIFMMILCLAALEVRSFAQNISSEDVKRLQLFLKRTLG
                     ARVPADAKIEVKGYEKSSIQGFKKGTFVIQSSKGSGDVAFLMSNDGRYLIFGEPIETQ
                     KFQDAGVAGMKKGTIPLGRQAVSILMTKDGKYIILGELIDSKVNPLDETMKKISLNDV
                     PIKGNKSAKVTVVEYSDFQCPFCKRATDMLPGILDQYKNKVKLVYKQLPLPNHPWAKD
                     AAIASECAFDQGNDAFWKFHDLLFQKQKDITVEKSKEQFKTFAKDVGLNTAKFETCVN
                     SPETATTVQNQIKEAQSIGISSTPTFVVNGMLVPGANPDGLKAAIDASLSGNI"
     gene            complement(1865..3166)
                     /gene="purB"
                     /locus_tag="VI935_08755"
     CDS             complement(1865..3166)
                     /gene="purB"
                     /locus_tag="VI935_08755"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HLE25724.1"
                     /translation="MIQRYSRPEMLRIWSDEAKYQNWLRIEIAVCEAWAKLGRIPFVS
                     LANIKERASFNATRVEELEKTFKHDVIAFLTCVSEYVGEESRYIHVGLTSSDVLDTSF
                     ALQLRDAAAIIVDDLKQVIEVLRGLAYKHKYTPMIGRTHGIHAEPITLGLVFALWYDE
                     MKRNLDRMERARDVVSVGKISGAVGTFANVPPFVEEHVCSVLGLKPAKVSTQIIQRDI
                     HAEYFLTLSIIASTIEKIALQIRHFQRTEVIELEEPFTQGQKGSSAMPHKRNPVLSEN
                     LCGLSRLVRSYAISALENIPLWHERDISHSSVERVIGPDATILVDFMLYRLRGLLDTL
                     EIYPENMEKNLWLTRGLIFSEKILLKLIDKGLSREKAYVLVQRNAMKSWGGKDNFKDI
                     ILKDEEIISCLSLHEIEECFDVKHDLKQVDYIFGRVFSESE"
     gene            complement(3163..3960)
                     /locus_tag="VI935_08760"
     CDS             complement(3163..3960)
                     /locus_tag="VI935_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLE25725.1"
                     /translation="MTTKLKLTHTSIGNIMKIRTLASGSSGNSLFVETRRTKILVDAG
                     LSHRQLTDRLKKMGVLMSDIDAVLITHEHTDHVAAIPQIKAPVYVSSSTVSLWKDKVK
                     DLKEFEPGSQFFLNDALITPFPVPHDALDPVGFTIETDHKKLGIVTDIGSVTALVRER
                     LRGSNALIIEFNYDEGMLLYGSYPWDLKQRIKSRLGHLSNEDAAELLQSLIHEELRYV
                     ILAHLSEVNNTPKKALESAHSAIIKNGSENIRLSVAQRNDLGEVAIV"
     gene            4034..>4432
                     /locus_tag="VI935_08765"
     CDS             4034..>4432
                     /locus_tag="VI935_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25726.1"
                     /translation="MDKEGNLFFEGNQITDPWVYRFFLRSLNETEDGRLLVVCENEHC
                     YIEVEDVPYIINDISVIRFEKEGIVHIELLFKGGYKEVLDPSTLHVGKDNILYCRVRK
                     GKFLARFNRRSYYHIVNFIEYNDKTGEFQIK"
BASE COUNT         1304 a          951 c          824 g         1353 t
ORIGIN      
        1 gggaggaggt gtgccgagaa actgggcaca gcaagttaca cctatgattg aattcataaa
       61 gcatggtgtt caaattttgg aagacaggga aatcggatat aactatggtg ttctcatcac
      121 tacggatcaa ccccagtttg ggggcttaag cggaagtaca ctcgaagaag ccatctcttg
      181 gaataaatat gcaccccaag caaggtttgc aactgttcat tgcgatatga ctatcgcact
      241 tccgatcatg gtgggagctg taaaggaatg gctgtagtgt ttattaataa ttttaaggaa
      301 gataaacgga atcttaaagt cagccggcct ttattcctct attgaattgt ttttcaattt
      361 gaatcaataa ccctgtagca agctacaggg tatggtctta attaaagaac cacaactgtc
      421 attgcgagga gccctgagcg aagtcgaagg ggacgaagca atctaaaatg ggagattgct
      481 tcacttcgtt cggaatgaca atacagcatt gtggaaaccc tgtagcaagc tacagggaat
      541 aatcaagtga aaaagtgaat caaaactttt aattttcaga taacggccgg ctgaccctgt
      601 atccatttat atgttacctg ataggctagc atcaattgcc gcttttaagc catcaggatt
      661 agccccagga acaagcatac cgttcaccac aaacgttggc gttgagctta tgccaataga
      721 ctgcgcctct ttgatctggt tttgaactgt tgtggctgtc tcaggggaat taacgcaggt
      781 ttcaaactta gctgtgttta aacctacatc ctttgcaaag gtcttaaact gctctttaga
      841 tttctctaca gtaatatcct tttgcttttg aaacagaagg tcatgaaact tccagaaagc
      901 atcgtttccc tgatcaaatg cacactctga agcaatcgcg gcatccttag cccatgggtg
      961 attaggcagt ggaagctgct tataaaccaa tttcacctta ttcttgtatt gatctaagat
     1021 cccaggaagc atatcagtgg cacgcttgca gaaaggacac tgaaaatccg aatactcaac
     1081 tactgtaacc tttgcactct tgtttccttt tatggggaca tcatttaaag aaatcttttt
     1141 cattgtttca tcaagtggat taaccttaga atcaatcaat tcaccaagaa ttatatactt
     1201 tccatcttta gtcattagaa ttgaaactgc ctggcgccct aaagggattg ttcccttctt
     1261 catccctgct acacccgcat cttggaattt ttgagtctct ataggttcac caaaaattaa
     1321 gtatcgccca tcattactca ttaaaaaagc tacatctcct gatcccttgg atgactgaat
     1381 tacaaatgtg ccttttttaa acccctgaat tgaactcttt tcataaccct ttacctcaat
     1441 tttagcatct gcaggcaccc ttgcccccaa ggtcctttta agaaagagtt ggagcctctt
     1501 tacatcttca gacgaaatgt tttgagcaaa gctccgaact tcgagcgcag caagacatag
     1561 aatcatcata aatattgaaa acaacttaaa tctcatttct acctcctcat atactaaaaa
     1621 gattgcgtta aatataccac cacttatctg aaaagcaacc aaaatcttga tttccgattg
     1681 tgtaagtctg atgtatgcct aactcaaatt ttggatttcg gaatttgaat cgttaatctc
     1741 atacagaact tcattagaag aacgttaaaa cactcacact ctaagtccgc aatttttaaa
     1801 accgtaactg atgtgtaatg tatttgtata tatctgtata tcaaggttta acagttgttt
     1861 ttcgttattc actttcacta aaaaccctac caaaaatata gtccacctgt tttaagtcat
     1921 gctttacgtc aaaacactct tctatttcat gaagagagag acaacttatt atctcttcgt
     1981 cttttagtat tatatccttg aaattatctt ttcctcccca gcttttcatc gcgtttctct
     2041 ggacaagaac ataagctttt tctcttgata gtcccttgtc aattaattta agtaatatct
     2101 tttctgagaa aataagacca cgagtaagcc agaggttttt ttccatattt tctggatata
     2161 tctcgagtgt atcaagaagc ccccgaaggc gatacagcat aaaatcaact aaaatagtag
     2221 catcgggacc gattaccctt tctaccgatg agtggctaat atcgcgctca tgccagagtg
     2281 gaatattctc aagcgcggat atagcgtaag agcgaactag cctcgaaagc ccgcataggt
     2341 tttcagatag cacaggattt ctcttatggg gcatggcgga tgaccccttc tgtccttgag
     2401 taaacggctc ctctagctct atgacctcag ttctttgaaa gtgtctgatc tgcaaagcaa
     2461 tcttttcaat cgttgaggct attatcgaga gcgttaggaa atactccgca tgtatgtcac
     2521 gctgaataat ctgagtggaa acctttgctg gtttaagccc aagaacactg catacatgct
     2581 cttctacaaa aggaggcacg tttgcgaagg ttccaacagc accggaaatt ttaccaacgc
     2641 ttacaacatc tcttgccctt tccatccgat cgaggtttct tttcatctcg tcataccaga
     2701 gtgcaaaaac aagacccaga gttataggct ctgcatgaat accatgggtt cttcctatca
     2761 tgggtgtata cttatgttta tatgcaagcc ctcttagaac ctcgatcacc tgtttcaaat
     2821 catcaactat tatcgctgca gcatccctta gttgaagtgc aaaggatgtg tccaaaacat
     2881 cagatgatgt aagcccaaca tgaatatacc tcgactcttc cccaacatat tctgaaacgc
     2941 atgtaagaaa ggctatgaca tcatgcttaa atgtcttctc tagctcctca acccgtgttg
     3001 cattaaaact tgcacgctct ttaatgtttg ctaaagatac aaacgggatc cgtccaagct
     3061 tagcccaggc ttcgcagact gcaatctcta tacgaagcca gttctgatac ttagcttcat
     3121 cactccagat tcttagcatc tcaggtcgcg agtatctttg aatcatacta ttgcaacctc
     3181 tcctaaatca tttctttgag caacagagag tcttatgttt tcgcttccgt tttttataat
     3241 ggcagaatgc gcgctctcta aggccttttt tggcgtgtta ttcacctcac ttaaatgagc
     3301 gaggattaca tatcggagtt cctcgtgtat gagactttga agaagttctg ccgcatcctc
     3361 atttgagagg tgccccaaac gactctttat ccgttgcttt aaatcccatg ggtatgaacc
     3421 ataaagaagc ataccttcat catagttgaa ctctataata agtgcatttg atccccttag
     3481 cctttccctt acaagagcgg ttacagaccc tatgtccgtt acaataccta actttttatg
     3541 gtctgtttca attgtaaatc cgaccgggtc caatgcgtca tgtggaacag gaaatggggt
     3601 aattaaggca tcatttagaa agaactgaga acctggttca aattctttta gatctttaac
     3661 cttatccttc catagtgaaa ctgtgcttga tgaaacgtaa accggtgcct tgatctgagg
     3721 aatggcggca acgtggtctg tatgctcatg tgtaatgagc actgcatcta tatcagacat
     3781 tagaacaccc atttttttaa gtctatcggt aagttgtcta tggcttaaac ccgcatccac
     3841 tagaatcttt gttcttcttg tttcaacaaa aagagagttt ccagagctac cacttgcaag
     3901 cgtacggatc ttcattatat tcccgatact agtatgagtt agtttaagtt tagttgtcaa
     3961 ctattaattt cctttatggt atcctttttt tatgcagggt acaggagaaa taatccgaaa
     4021 gcactattat ttcatggata aagaaggaaa cctctttttt gaaggaaatc agatcacaga
     4081 cccctgggtg tatagattct tccttaggtc tttaaatgaa acagaagacg gtcggttgct
     4141 tgtcgtatgc gaaaacgaac actgctacat agaggttgag gacgtacctt atataataaa
     4201 tgatatatca gttatacgat tcgaaaagga gggaatagta cacatcgagc ttctttttaa
     4261 aggcggatat aaagaggtgc ttgacccctc caccctgcat gtcggtaaag ataacatcct
     4321 ttactgccga gtaagaaagg gcaaattcct agcaagattt aacagaaggt cttattatca
     4381 tatagttaac tttattgaat acaatgataa aacaggagaa tttcaaatta ag
//
LOCUS       DATDRL010000260         6439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3250206, whole genome shotgun sequence.
ACCESSION   DATDRL010000260 DATDRL010000000
VERSION     DATDRL010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3250206"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VI935_08770"
     CDS             <1..185
                     /locus_tag="VI935_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25727.1"
                     /translation="TLEEVESMSPNVISDIVGLEIAMNRPKCATLGLTSVKMAISEWR
                     RKKMLDEIEEERSLSR"
     gene            complement(339..656)
                     /locus_tag="VI935_08775"
     CDS             complement(339..656)
                     /locus_tag="VI935_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HLE25728.1"
                     /translation="MNKGRWVFILLIAFFTLSSYVLTETNKININTATVEELVSLPGI
                     GNKIANRIIEYREKNDNFKTIEEIMNVKGIGKKKFKKIKQLITVGDETTPARATPSPT
                     PTK"
     gene            894..2114
                     /gene="coaBC"
                     /locus_tag="VI935_08780"
     CDS             894..2114
                     /gene="coaBC"
                     /locus_tag="VI935_08780"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HLE25729.1"
                     /translation="MFRIREKNIILGVTGGIAAYKACELIRRLINEESNVQVIMTKNA
                     TEFVSPLTFQTLSRKLVATDVFDLEWEAEIGHISLADSADLLVVAPATANFIGKVASG
                     IADDLLTTVLMAVRSPVMICPAMNVNMYGNPVVQENIEKLRKRGFIIVEPAEGDLACG
                     YEGKGRLPEPDDIIEEIEKVFAPKNLRKEKILITAGATREFIDSVRFLSNPSSGKMGY
                     ALAREGWMRGADVILVSGKADVLPPRGVNLINVVSVSDMYEAVISHLDWATIVIKAAA
                     VGDYTPSNTFQGKLKKGSRETTLELKRTVDILSEVGKKKNGRVVVGFAAEIENIVENA
                     LEKLRKKNADLIVANDISKPGSGFGEDTNIVYFVDKFGKVEEFSLMTKTEIAQRVYDK
                     IIDIKRHSAQLGDS"
     gene            2216..3418
                     /gene="ilvA"
                     /locus_tag="VI935_08785"
     CDS             2216..3418
                     /gene="ilvA"
                     /locus_tag="VI935_08785"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HLE25730.1"
                     /translation="MVTLKEIEEASKRLKCIVYKTPIVYSFSLSELSGANVFLKLENL
                     QRTGSFKIRGAYNKVYNLRSAAKNVVTASAGNHAQGVALAAKLLGMKATIVMPEGTPI
                     NKILATKHYGADVVLHGNSFEDAYVYAKQLTESSSGVFIHAFDDPEVIAGQGTIGLEL
                     IETLQNIEVVFAPIGGGGLISGISIAVKENNSKIKIIGIQSTSSPSMLVSKEKGTVVE
                     IISKETLADGIAIRKVGAIPFNIVQNYVDDIMTVDEQEIEDALLLLTERKKIVVEGAG
                     AVALAGLLKRKESFYKKNAAIIVSGGNIDINTLAKIMERGLIKRGRFMKLELELPDVP
                     GSLAKLATLLGSVKANIVQIIHDRFSRELQLNKAIAEISLETQGFEHQEEILKLLREN
                     GYDPKGKE"
     gene            complement(3579..3866)
                     /locus_tag="VI935_08790"
     CDS             complement(3579..3866)
                     /locus_tag="VI935_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HLE25731.1"
                     /translation="MAKPMTKSQITDHLAKKASITKKMAAYILDEIASLAHREAKNTF
                     TLPGIGKLVLVNRKARIGRNPATGAEIKIPAKRVVKFRVAKACKDAALGKK"
     gene            4380..4592
                     /locus_tag="VI935_08795"
     CDS             4380..4592
                     /locus_tag="VI935_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25732.1"
                     /translation="MERPDNFIGVNLSSVSLTVSVRTHPWRVVDPPTSFENSADGFCN
                     LLAWLHKHSHKPDNSVVCMEATGSCQ"
     gene            complement(4890..>6439)
                     /locus_tag="VI935_08800"
     CDS             complement(4890..>6439)
                     /locus_tag="VI935_08800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLE25733.1"
                     /translation="KIEIRITVIDKDGVVIGDSEEDPARMENHAKRPEVIDAISKGSG
                     KSLRYSTTVKKGMFYVALPVKDQTGGIKAVVRTALPLSFLEKVFSPVKFKIIYLGLIL
                     IAFALFLSLASTRALTKSLDKTIKLSEEIAKGNFNVSIPISDKKGEIGKLNLALSTMA
                     KKLDELFKQVSLEKTQLETVLSTMSEGVMVVRGDGKITLINNALKDMLGIKDDWIEKP
                     YWEIFRIREIMELVENVIKKQLAEKKEISVLYPTERYFLASAIPLNSVEKEAIFVIFD
                     ITEFKRLERIKADIVANVSHELRTPLTAIKGYIETLEEGAYENPDERNHFLSVIKRHT
                     DRLINIVSDLLLLSEIERKSAPWGEESKTAFEDVDFKEVVHSSLEALKSRVEEKSLHV
                     SLNIQDGLPKYKGDRFLLEQMLINLIDNAVKYTPERGTIGVQVNQSDSHLGIEVFDRG
                     VGIPKEHLPRIFERFYRADKTRSRKLGGTGLGLSIVKHVVIMHGGEIQVESEVGKGSR
                     FIITLPV"
BASE COUNT         1998 a         1231 c         1256 g         1954 t
ORIGIN      
        1 aaacacttga ggaagttgaa tcaatgagtc ctaatgtcat ttcagatata gtaggactag
       61 aaatagcgat gaaccgtcca aaatgcgcga ctttagggct taccagtgtt aaaatggcta
      121 ttagcgaatg gcgaagaaaa aagatgctag atgaaattga ggaggaaaga agcttatcaa
      181 ggtaagaatc ctccttcatg tggagactac attttgaatc caacacaaaa caaaaattgt
      241 agccgaaggc ttcagccttg catgaacagt atctttgtca ttgcaatgat aacatgaaac
      301 cctgtactag ctacagggaa ttgccgagtc acagaaaatc attttgtagg tgtaggtgaa
      361 ggagttgccc gcgctggcgt agtttcatca ccaacagtaa ttagttgttt tatcttttta
      421 aattttttct tacctattcc ttttacattc attatttctt caattgtttt aaaattgtca
      481 ttcttttctc tatactctat tattctattc gctattttat tacctatacc tggcaagcta
      541 acaagctcct caactgtagc tgtattaata tttatcttgt tagtttctgt aaggacatac
      601 gatgacaaag tgaaaaatgc tattaaaagt ataaaaaccc atctaccctt attcatgttt
      661 ttgcctggct taatatcatt gataattata tttgcacatc atataaattg taaagactct
      721 ttatatccat ccgataatta aatacgacat acgacgggct aattctgcat tctgataatg
      781 caatatgaaa ggaattgagc atgcacataa atagtatata agtaagctcc catcagtttg
      841 cctcatctca taatctttac tataataata aggaatattt aaggaaaaat aagatgttta
      901 gaattaggga gaagaatata atcctaggtg ttactggtgg cattgccgct tataaagcct
      961 gcgagcttat aagaaggctc ataaacgaag aaagcaatgt acaggtaatt atgactaaga
     1021 atgctacgga gtttgtttcc ccacttacgt ttcagaccct ttctagaaag ctagtagcaa
     1081 cagatgtttt tgatctcgaa tgggaagcag aaataggcca cataagcctt gcagacagcg
     1141 cagaccttct cgttgttgct ccagctacag caaattttat aggaaaagtg gcatctggga
     1201 ttgcagatga tcttcttact acggtactta tggcggtacg gtcacctgta atgatatgtc
     1261 ctgcaatgaa tgtaaacatg tacggcaatc ccgttgttca ggaaaacatc gaaaagctga
     1321 gaaaacgcgg atttataata gtagagccag cagaaggaga cctagcctgt gggtacgagg
     1381 ggaagggtag acttccggaa ccggatgata ttatagaaga gatagaaaag gtatttgccc
     1441 ctaaaaacct gagaaaagaa aagatcttaa ttacagcagg agctacaaga gaattcattg
     1501 attctgtgag atttctttcg aatccttcaa gcggaaaaat gggatacgca cttgcaagag
     1561 aaggatggat gagaggtgcg gatgtgattc tagtctcagg taaagctgat gttttaccac
     1621 ctagaggcgt aaatctaatt aacgttgtta gcgtatcaga tatgtatgag gcagtaataa
     1681 gccatcttga ttgggctacc attgtaataa aagctgctgc agtaggtgac tatactcctt
     1741 caaatacctt ccaaggaaaa ctaaagaagg gttcaaggga gacgacgctc gagcttaaga
     1801 ggacggttga tatactatct gaagttggta agaaaaagaa cggcagggta gtagtagggt
     1861 tcgcggctga aatagaaaac atagtagaaa acgcgcttga gaagcttaga aagaagaacg
     1921 ctgacttaat tgtagcaaat gatatatcga aacccggatc aggatttggc gaggatacta
     1981 acatcgtcta ttttgtagac aaattcggaa aggttgagga attttccctt atgactaaaa
     2041 ccgaaatcgc ccaaagggtt tatgataaga ttattgacat taaacgtcac tctgcgcaat
     2101 taggggattc gtgactcgga tttagtgatt cgaatttgtc ttagcttccg acgaaagttg
     2161 aactaaccac tattcacgag acacaatcca cgaacaatat ctcgcgaatc aatccatggt
     2221 tacactcaaa gagatagaag aagcctcaaa aagattaaaa tgtatagttt ataaaacacc
     2281 tattgtctac tcattttctt taagtgagct ttcgggggca aacgtattcc ttaaactcga
     2341 aaaccttcag agaactggct cttttaagat tcgcggggct tataataaag tttacaatct
     2401 gagaagcgct gcaaagaatg tagttaccgc atcagctgga aatcatgcac agggcgtagc
     2461 acttgccgct aaacttcttg gcatgaaggc aaccatagtc atgcctgaag gaactcccat
     2521 taataaaatc cttgctacaa aacactacgg agcagatgta gttcttcatg ggaattcgtt
     2581 tgaggatgct tatgtatatg cgaagcagct tacggagtca tcaagtggag tttttatcca
     2641 cgcctttgat gatcctgagg taatagcagg tcagggtact attgggcttg aactcattga
     2701 aactcttcaa aatattgaag tagtgtttgc acctattgga ggtgggggtc taatctctgg
     2761 aatctcgatt gcggttaaag aaaataattc aaaaataaaa attataggta ttcagtcaac
     2821 ttcttcacct tctatgctag tctcaaaaga aaagggtacg gtcgtagaaa ttatttccaa
     2881 ggaaaccctt gccgatggaa ttgctatcag aaaggttgga gctattccat tcaatatagt
     2941 tcaaaactat gtagatgata ttatgacagt tgacgagcaa gaaatcgagg atgctcttct
     3001 ccttttaacc gaaagaaaaa agatagtagt tgaaggtgct ggcgctgtag ctcttgccgg
     3061 actcctcaaa aggaaggaga gtttctacaa aaagaatgct gcaattatcg tgagtggtgg
     3121 aaacatcgat attaacacgc ttgctaaaat catggagagg gggcttataa agcgaggacg
     3181 gttcatgaaa ctagaattag agcttcctga tgttcctggc tctttagcta agctggctac
     3241 tcttcttggc tcggtcaaag caaacatagt acagattatt catgacaggt tttcaagaga
     3301 acttcagctt aataaggcca tagcagaaat tagccttgaa acgcaaggtt ttgaacacca
     3361 ggaagaaatt cttaaactcc ttcgagaaaa tggatatgac cctaaaggga aagaatgaga
     3421 catctagcct aatggcttta tacccttctt aatgtaaaaa ttgtattaac cgccaacgga
     3481 ttagcgataa taacccccct ctatctcgct aacggtgggc gaagcacaat tgtttcggat
     3541 taatcagata atgttactaa ttaaatagaa agaacctact atttttttcc gagtgcggcg
     3601 tctttacatg cctttgcaac tcgaaattta actactctct ttgcaggaat ctttatctcc
     3661 gcaccagttg cgggatttct tccgattctg gctttgcggt ttactagcac aagcttgccg
     3721 attcctggaa gtgtgaaagt atttttagct tccctgtggg caagagatgc aatctcatct
     3781 agtatgtaag cggccatctt ttttgtgata gaagcctttt tagccaaatg gtccgttatc
     3841 tgggacttag tcattggttt cgccatattt cctcctttaa aactgtgtga ttgtttgtca
     3901 tattacagaa gttattagaa aaatcaatat gttttgttta tttaataaaa aatctttctg
     3961 aacttaacaa gttggccgtc gagcttcgta gaagttttgg tagtatctat acccgaaaac
     4021 tcagtgtgta attttgagcc ctggagagat caacatagcc ttcataacca aaagcaaaag
     4081 gtaaattcag caaacgtaga ggaaacctct acgttccata aaattattca tggaaggccc
     4141 atactacgcc aagctccatt agacttttat atagtaatga ttttaacttt acatgttaaa
     4201 actaaccaag gtggaataag tctatgacag gctcaaagct atagcttatg tcctgaataa
     4261 gaccccaccc tgaagggtag ataaaactat atagtattta agtctcttaa tgactattag
     4321 catggagcca gttctaacta cccattggta aaagtaagta aaagatagga ggtgagaaga
     4381 tggaaagacc tgacaacttt atcggagtga atttaagctc agtgtcatta accgtatctg
     4441 taagaacaca cccatggaga gtggtcgacc ctcctacatc ctttgaaaac tcagcggatg
     4501 gtttttgtaa cttattggca tggttacata aacacagtca taaaccagac aacagtgtag
     4561 tttgtatgga agctacaggt tcttgtcaat agtaccactt tagttctttt ttttgctcgc
     4621 atcaattccc aaggagcagt ttacgctcac ttaaaagaag atggcagcga tccttgccct
     4681 aacccgtctc gatatgtcac cttctgttaa atttaaataa agattatagg agcttagatt
     4741 gaatttgcta aatttgtagg gcggaacgca accaccctga gtaacgctgt aatatagttc
     4801 cgtttgtggt taaatagaaa caaaaagtta caggatataa ttcagaattt gattagaagc
     4861 ggtctcataa ataggaatgg ggacaccttt cacacaggaa gtgtaattat aaatctgctt
     4921 ccttttccta cttcactttc tacttgaatt tctccaccgt gcataatcac cacgtgcttt
     4981 acaatgctaa gcccaagccc agtaccgccg agcttccttg aacgcgtttt gtcagccctg
     5041 tagaaacgtt caaaaattct aggtaagtgt tctttgggaa taccaacccc acgatcaaaa
     5101 acctcgatcc caaggtgtga atcactctga ttaacctgaa ctccaatagt gcctctttca
     5161 ggcgtatact ttacggcatt atctattagg tttataagca tctgttctaa cagaaatcta
     5221 tctcccttat atttgggaag accatcctga atgttaagag aaacatgcag gctcttttct
     5281 tcaactctac tttttagcgc ctcaagggat gagtgtacaa cttctttaaa atcaacatcc
     5341 tcgaaggcgg tctttgattc ttctccccat ggagcgctct ttctttcaat ctcagatagc
     5401 aagagaaggt cagaaacaat attgattaat ctatctgtat gcctttttat tacgctaaga
     5461 aaatgatttc tctcatctgg attttcataa gctccctctt ccagggtttc aatatagcct
     5521 ttaatggctg taagaggtgt tctaagttcg tgagacacat ttgcaacgat atcagctttt
     5581 attctctcta gtcttttgaa ttcagtaata tcaaatatca cgaagattgc ctctttttcc
     5641 accgaattta agggaattgc gcttgctaga aaatatctct cagttgggta aaggactgaa
     5701 atctctttct tctcagccaa ctgtttttta ataacatttt ctaccagttc cattatttct
     5761 ctaattctaa aaatttccca gtacggtttt tcaatccaat catcctttat tccaagcatg
     5821 tcttttaagg cattatttat aagtgtaatt ttgccatcgc ctcttacaac cattactcct
     5881 tcactcatcg tgctgagaac tgtctcaagc tgagttttct caagagaaac ttgtttaaat
     5941 agttcgtcta attttttagc cattgtgctt aaggccaagt taagctttcc gatttctcct
     6001 tttttatccg aaatagggat acttacatta aaattccctt ttgcaatctc ttcagagagc
     6061 ttaatagttt tatcaagtga ttttgtaagt gccctagttg atgcaagtga aaggaaaagc
     6121 gcgaaagcta ttaaaatcaa gcctagataa attatcttga attttacagg agaaaacacc
     6181 ttttccagga agctaagtgg aagtgctgtg cggactacag ccttaattcc gcctgtttga
     6241 tcttttacag gaagcgccac ataaaacata cccttcttaa cggttgtgct atagcgtaga
     6301 ctttttccac ttcctttaga aattgcgtct attacttctg gtctctttgc atggttttcc
     6361 atcctagcag gatcctcttc agaatcaccg atcacaactc catccttatc tattactgta
     6421 atccttatct ctatttttt
//
LOCUS       DATDRL010000261         9894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2986735, whole genome shotgun sequence.
ACCESSION   DATDRL010000261 DATDRL010000000
VERSION     DATDRL010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9894
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2986735"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            476..3097
                     /locus_tag="VI935_08805"
     CDS             476..3097
                     /locus_tag="VI935_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25734.1"
                     /translation="MSYSDLLKPRVDVLKEDGIEGIIDLANLEDPKGKKLETDPQRFL
                     TLTYPTADVRRVIQKLSDRFSGHKDAPGLFLFEGLKGSGKSHLLLMIYHLFKNYKIAK
                     RWLDAHKMYCELPDNAVVVVNKFTDLPLDSIWDFVFKQLTNQRPKKSVVQPSLAEVEK
                     ILGDKRLILILDELEQGIRVISEPSIKAQNIAFLQMLSEWANRSDKVTVFASIYSDQE
                     EPGDTLKRGPSCRVQFTYSPDKVKVVLHRLFENYLEFKHDACIPTVDSYLNAWRRQHL
                     SLSEEYRSRMLLTYPFLPELLELILERVPARGGFQSVRGALGFLANMVRLTHGKVDLI
                     TAAHATLQDREVAVRLQDLDPSGDLISRAKGNLNELKDHLLVSEIGATVMLYTLTGSG
                     RARGVSREELIKHVLNPKVDINDFGKTLLALQKYASHFHIEEGRYFFDPEENADAKVE
                     FRSLTIDPEGSKARSFLHVLWRDEIFKEPNCVIYQGEEETKSKLDELDKNRLRYVLSP
                     RKLNQEERHELFHGVPIRNQVILLEPKDQKFNLNIHSDLLKWAKRQLAAKELTETAKD
                     STRRSEYERIAREDKSHCIEAIKRAGLVFTKWEEYGQTANEDRVEEEAISGNSREDVL
                     NFLSQKLFPIQLFEEHLLSRLHEIIGSTVKDIDRQYRSTIGFHVPTTVRTVSQALREL
                     CKNGQISIRHPRGNFCRENPNLTETELFDATIGEPFETPIVRSSILKPTEEEGETPFI
                     ETEQEDTITVTPLPAGSLRKQEISIPPQESSGSLRQTIALQLQKFPNAKIIRIRTTIF
                     LEKKTGDLSSLPASIRGSLSGQGTLSAEITITKEGEFTKGEVETIIESLPTLPGADYG
                     ARLNLLIPSTEDEKNND"
     gene            3090..4076
                     /locus_tag="VI935_08810"
     CDS             3090..4076
                     /locus_tag="VI935_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25735.1"
                     /translation="MTNLILNNFASLIRPGPRIEWLEKWLLGEVWTTVRYQSLSPIEY
                     LNDGESKVNELEEIIAQAAYRLYDEFLEEPRQGRNILGFIENDESSALVIFDGLSLRE
                     IPVLLNLAQKSNFIVQEVGVSYSALPTETISYIEQRLKLGSVAPSQLPRRREIKEKGI
                     SAYYYDSQSQRHLLDPDARKLFLWSAFPDNTYSDSGARFAQHFEQIHTLLETAWLNTV
                     QQIPRGRRILITSDHGYVYLGAGLTFARSNSELRPLSEYLGGERHRDLTEEGNQPPEH
                     PDLAVYPNHKVAVLRGRIQTHPPGKSATRLYKHGGLSIMEMLVPWIVLEAKT"
     gene            4076..7177
                     /locus_tag="VI935_08815"
     CDS             4076..7177
                     /locus_tag="VI935_08815"
                     /inference="COORDINATES: protein motif:HMM:NF018357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HLE25736.1"
                     /translation="MADSKRNVNSIKYAIEDSFPIVEINRLAIPERNAFKPIYKMHKW
                     FARRASCVFRAILLGCMKPVPLDENGNPIKSGAEVIMEEFYKDHTNDPDTKGKVVLDP
                     FMGGGTTVVEALRLGCKVIGIDLNPVAWFIVKTEVKPVDIDELKASFERLAERKVVWN
                     GKLVKQTLLERYKTECPCCGAGRVEADIIYTFWVKSAICTNPLCKKEVPLFSDYIIAA
                     KSPSIRYNRDVECPECKKTFDWEIEPASLIAEPGLCIVSPSYSAGSGRTSARWAFSAN
                     DKVECPWCKKIVKAQIKHPKAERKKVSLTVLLCPHYYSVWQWRGNLPDEVSCPVCKKD
                     YNPVVGNIPRSGWFLCPSCGHTDRIINSIRNLPKDQLLPLRPYAIEGYCSFCAGDIEE
                     EVESPQLTLDGANSKIEIKKTKLTDHICQLTKNNGKFFKRITPSDLARYQKACEMWEK
                     EKGNLPYPRQKIPDGQETHRLLEHHYHYWHQMFNPRQLLCLSLLLKAIDEEPNQVLKE
                     MLLSAFQGSLESNNTFCRYTIAGGNKSQGIFSRHDFQPKLTFTENNPWGTEYGHATFY
                     NKFELIVEGKKWAFVTSDRCFTGRFSKEGKPILEDVQSGEAVYSTEKQVFLMAGDSKS
                     LGSLIQDGEAHFVVTDPPYASNVNYSELADFFYVWLRLSLSKTYPYFAPELTPKVQEI
                     IVNPTRGHTEEDFKLGLQAVFEECRRKLLQNGLLLFTFHHSEGKAWEALLEAVCEAGF
                     EIDSVYPIQGEAETSLHLLEKEAIAYDLIHVCRKRKVEQEIKKRSWAGIRQEIRRRSR
                     EEIKAIEAGRYGQELSPADVNIVLIGKCLELYSRHYGAVVDHEGKEVTLKQALEEIRM
                     MVDQLLTREQPLPSELSDIDPESYVYLTSLCDRKEVKSDDVHKATRGILEPESLIKAG
                     IMIKGRAGRGRTHEVKQPSERFNSLLEKFKETPLPQQDLFGEIEAPKMKGNIYFIDYI
                     HFLMALVEGGENIIPWLEGFRGETPRLRAACEYLMARNRSFTPTLKKIIDLIEVGPLF
                     QTR"
     gene            7182..7613
                     /locus_tag="VI935_08820"
     CDS             7182..7613
                     /locus_tag="VI935_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25737.1"
                     /translation="MPGYQVVEIVNLRPNKDNGIMPYENKIGFLDFLREIAQEPSPFP
                     KFHRLQVSGLEEVLYAGRPNHEELALKIHKLLRSAAQNLEKRLIEVQIVFKGKLMRGE
                     NLWVEYRNTKLPIGHIFGTPIRQVDAKGNPFFRSNFNLTNG"
     gene            7632..>9894
                     /locus_tag="VI935_08825"
     CDS             7632..>9894
                     /locus_tag="VI935_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HLE25738.1"
                     /translation="MSEHIQKTINAGEKVKAYGFEHLGTGEVIRLRESGKTYQVDVVF
                     DCVDGRRLETIPLDRLERVPDLFERISTGDVDSPLDFFLKQLAYQLPLNNAGGELSNS
                     RTDLLPHQILLTRDIVESQRRRFLIADEVGLGKTIETGLIIRELLARKDAERILIICP
                     AGLTRNWQQELRDCFRLHFDILGIDFNDTTSLSWETHHLVIASIDTIKQTRRIERLKH
                     APKWHIIVFDEAHHLSRKRYGKKIDYTQNYRLAEALRGYTRDMLFLSATPHQGDAFQF
                     WSLIQLLDDQLFESPESMIDHRGLLNRVMFRRIKREVTDAEGNPIFMRRHVHTQTFPL
                     AARERWFYDQLTEYLREGYGVAGIGEERTTSKQRAVGFVMAIFQKMMSSSPRAIRQAL
                     RRRLLVLLAREQMTLERKIQEVRATGLADKMLNIQEEMRGLAIKINSISTSLEQMALA
                     DAYIAKVKQRLARKGYAGEETTSWALDSEEEAEDIIYAEAVIPNESQKVRELIKLAPE
                     GPDRKFDTLIRAIEQVRRLSPQEKIIVFTQYRETLEFLNEELSKLYGEEKVATLKGGP
                     LEDKIAAVEAFWEPDGAQFLISTSAGGEGINLQVCNIMFNYDLPWNPMAVEQRIGRIH
                     RYGQTDTVQVYNLVAEDTVEERIYGLLEEKLLDIAKTIGKLDPVTGQVVEDFRSEILG
                     FLGSSPNYQDLYKKALIDKDYQRTEQEIVEAIEKAQKASEALRYLSQDLESFNFGRYR
                     NMKGPFTLDDLGLF"
BASE COUNT         3113 a         1860 c         2185 g         2736 t
ORIGIN      
        1 gtaactaaaa tggaggtaaa gttggccagg cgtaaaagga gtatgaagtc aatgggttcg
       61 cgtctcaaca tttgacattc attcacttgt tatgtcaaat gttgagacct gaccccgttc
      121 gtttgtctta ctgaattaat agaaaaggtg taggaattat tatatcttag gtcgcaggtt
      181 cgatttcttt catctccatc tgtttatgct ttttcagttt aatcgggaat aaatattacg
      241 cctgttctaa ttaagtcaat atgcatgcca gacccaaatt tgttcaactc tataaattaa
      301 aatatactcc tataaacaaa atcataataa ttttaacctg gtctagagta tagttaattc
      361 tgttctaaac aggctccaag aaaattgctg ctttgtgaaa gtttggggta tgaagttaca
      421 cgttatttgg tgggtgtagt ataatttaaa aaattctaaa cttaaggata cagaaatgag
      481 ttattcagac ctattgaaac ccagagttga tgttttgaaa gaagatggaa tcgagggaat
      541 tatagatctt gcaaatcttg aagatcctaa aggcaaaaaa cttgaaactg atccccagcg
      601 gttcttaacc ttaacttacc ctaccgcaga cgttcgcaga gttattcaga agttatctga
      661 tagattttcc ggtcataagg atgctccagg attatttctt tttgagggac tgaaaggaag
      721 cggaaaatct cacctacttc ttatgattta tcatttattt aagaattata aaatagcaaa
      781 gaggtggctt gatgcccata agatgtattg tgaacttcct gacaatgcag tagtggttgt
      841 aaataaattt accgacctcc cccttgattc gatttgggat tttgtcttca agcaattgac
      901 aaatcagcgt cctaagaaga gtgttgtcca accaagtctc gcagaggttg agaaaattct
      961 tggagataag aggctgattc ttattttgga tgagttggag caaggtatac gtgtaataag
     1021 cgaaccatct ataaaagcac agaatatagc attcctccag atgctttcag aatgggcaaa
     1081 ccgttccgat aaagttacag tttttgcaag catctatagt gaccaagaag aaccaggaga
     1141 tacccttaaa agaggaccta gttgccgagt tcaatttact tactctccag ataaggtaaa
     1201 agtagtgctt catagacttt ttgagaatta ccttgaattc aaacatgatg cgtgcattcc
     1261 aacggttgat agttacctga atgcgtggcg tcgacaacac ctttctttat ctgaggaata
     1321 caggtcaaga atgctactta cttacccgtt tttacccgaa ttacttgagc tgattttaga
     1381 aagagtgcca gctcgaggag gtttccaaag cgttcgaggt gcactaggtt ttctggcaaa
     1441 tatggtcagg ttaactcatg gcaaagtcga tttaataact gcagctcatg ccacactaca
     1501 agatagagaa gtagctgtaa gacttcaaga tcttgaccca agtggggatt tgatttcaag
     1561 agcaaaagga aatctaaatg aattaaaaga tcatctgttg gtatcagaga ttggcgctac
     1621 cgttatgctg tatacactta ctggttcagg tcgagcacga ggtgttagcc gagaagaact
     1681 gataaagcat gttctaaatc cgaaggtgga tataaatgac tttggaaaga cacttctcgc
     1741 cctacagaaa tatgcctccc attttcatat tgaggaaggc agatatttct ttgatcccga
     1801 agagaatgca gatgctaaag ttgagttccg ctctttaact attgatccag agggaagtaa
     1861 agctcgcagt ttccttcatg ttctttggcg tgatgaaatt ttcaaggaac caaactgtgt
     1921 tatctatcag ggagaggaag aaacaaagag taagctagat gaacttgata agaatcgcct
     1981 tcggtatgtt ctttctcccc gtaagttaaa ccaagaagag cgacatgaac tctttcatgg
     2041 tgttccgata cgcaatcaag ttattttact tgagccaaaa gatcaaaaat ttaaccttaa
     2101 tatccacagt gaccttctta aatgggctaa aagacagctt gctgctaagg aacttactga
     2161 gactgccaag gattcgacac ggagatccga gtatgaaaga atcgcacgag aagacaaaag
     2221 tcactgcatt gaggcaatca aacgagcagg tctcgttttt actaaatggg aagaatatgg
     2281 acagacagca aatgaagacc gagtcgaaga agaagctatt tctggtaata gtcgagagga
     2341 tgttcttaat ttcctttctc agaaactgtt tcctattcaa ttatttgagg agcacctatt
     2401 atcccgttta catgaaatta ttggtagtac tgtaaaagat atagataggc aatatcgttc
     2461 tacaattggt tttcacgttc ctacaactgt gagaacggtt agtcaggctc ttcgagaact
     2521 atgcaaaaat ggacagatta gtatcaggca tccgagaggt aatttttgca gagagaatcc
     2581 aaacctcaca gaaacagaac tctttgacgc aactataggg gagccttttg agacgccgat
     2641 tgtccgttcc tcaatactta aaccaaccga ggaagaagga gaaacaccat tcatcgaaac
     2701 cgaacaagaa gatactatta cagttacgcc tttgcctgca ggttccttaa gaaaacaaga
     2761 aattagtatc ccacctcaag aaagcagtgg gagtcttaga caaactatag ccctacaact
     2821 tcagaaattt cctaatgcaa agataatacg catacgcact acgatatttt tagagaagaa
     2881 gacaggtgat cttagttctc ttcccgcttc aatcagagga agtcttagcg gacagggaac
     2941 tctttctgca gagattacca ttactaaaga aggtgagttt actaaaggag aggtggagac
     3001 aattattgaa tcgcttccga cactgcccgg tgcagactac ggagcacggt tgaaccttct
     3061 tattccatct accgaagacg aaaagaacaa tgactaattt gattttaaat aattttgcaa
     3121 gcttgattag accaggaccc aggattgaat ggttagaaaa atggctgtta ggagaggtct
     3181 ggacaactgt acgttatcaa agcctttccc caatagagta tttgaatgat ggtgaatcaa
     3241 aggttaatga gcttgaagag atcatagcac aagcagcgta tagactttat gacgaatttc
     3301 ttgaagaacc ccgacaggga aggaatattt tgggttttat agaaaacgat gaatcaagcg
     3361 ccttggttat ctttgatgga ttgtcacttc gagagatccc cgttcttcta aatcttgccc
     3421 aaaaatcaaa ttttatagtt caggaagttg gagtatctta ctcagcattg cctacagaaa
     3481 ccatttctta tatcgaacaa agattgaaat taggtagcgt agctccctct caactgccaa
     3541 gaaggagaga aattaaagaa aagggaattt ctgcatatta ctatgatagt cagagccagc
     3601 gtcaccttct tgatccagat gcaaggaagc tttttttatg gtcagctttt ccagataaca
     3661 cctacagtga tagcggtgcc cgttttgctc agcactttga acaaattcat acactccttg
     3721 aaactgcctg gcttaacact gttcaacaga ttcctcgagg gcgaaggatt ttgataacca
     3781 gtgaccatgg atatgtgtat ctgggagcgg gcctaacctt tgcgagatca aattctgaat
     3841 taaggccact ctcagaatat cttggaggtg agcgtcacag agacctaacc gaggaaggta
     3901 accaaccacc cgaacatcct gatctagcag tttatcctaa ccataaagtt gcggttttga
     3961 gagggcgcat tcaaactcat cctccgggta agtcagcaac tagactatac aaacatgggg
     4021 gcctttcaat aatggaaatg ctagtgcctt ggattgtttt ggaggcaaag acctgatggc
     4081 tgattccaag cgaaatgtta attcaataaa atacgcaata gaagactcct ttcctattgt
     4141 agaaataaat cgtttagcga ttcctgagag aaacgccttt aaacctatat ataaaatgca
     4201 taagtggttt gctcggcggg cctcatgtgt gtttagagca attcttctcg gttgcatgaa
     4261 acctgttcct cttgatgaaa atgggaatcc tattaaaagt ggcgctgaag ttattatgga
     4321 agaattctac aaggatcata ctaatgatcc agatacaaaa ggaaaagttg ttcttgatcc
     4381 ctttatgggt ggtggcacta ctgtagttga agcgctgagg cttggatgta aagttattgg
     4441 aattgacctt aatccagttg cctggtttat agtaaagaca gaagttaaac cagttgatat
     4501 tgatgaactt aaggcatcat ttgaacgcct cgcagagcgt aaggttgtct ggaacggtaa
     4561 attggtcaag cagacgcttc tcgaacgata taaaactgaa tgcccttgtt gtggcgctgg
     4621 aagagtagaa gcggatatta tctatacctt ctgggttaaa tcagctattt gtactaatcc
     4681 gctgtgtaaa aaggaagttc ctcttttctc agattatatt atcgcagcca aatctccctc
     4741 aataagatac aaccgtgatg tcgaatgtcc cgaatgcaaa aagacctttg attgggagat
     4801 tgaacctgcc agtttaattg ctgaacctgg actttgcata gtgagcccat cctattctgc
     4861 cggatctggt cggacaagtg caaggtgggc attttctgcc aatgacaaag tagaatgccc
     4921 ctggtgtaaa aagattgtaa aagcacaaat caaacatccc aaagcagagc gcaaaaaggt
     4981 ttcactcaca gttcttcttt gtcctcacta ctactctgta tggcagtgga gaggaaatct
     5041 tcccgacgag gtcagttgcc ctgtttgcaa aaaggattat aaccctgtgg taggaaatat
     5101 tcctagaagc ggttggtttc tttgtccttc ctgtggtcac acggatagaa taataaactc
     5161 aatccgaaat ctaccaaaag accaactcct tcctttgcgc ccctacgcca tcgaaggtta
     5221 ctgttctttt tgtgcgggtg acatagagga agaggttgaa tcaccccaat taaccctcga
     5281 tggtgctaac tcaaaaattg aaataaaaaa aacaaagtta actgaccata tctgccagct
     5341 tacaaagaat aatggaaagt tttttaagcg tattacgccc tcagacctgg ctaggtatca
     5401 aaaagcctgt gaaatgtggg aaaaagaaaa agggaatcta ccatacccaa gacaaaagat
     5461 tcctgatgga caggaaactc atcgtttact cgaacatcat tatcactact ggcatcaaat
     5521 gttcaatcca cgtcagttac tttgtctttc attattactg aaagcgatag atgaagagcc
     5581 aaatcaggta ttgaaagaaa tgttgcttag tgcgtttcag ggttcactag aaagtaacaa
     5641 tacgttctgt cgctatacga ttgctggggg taacaagtcc caaggaattt tctctcggca
     5701 tgatttccaa cccaaattga cttttactga aaacaatcct tggggcacag aatacggtca
     5761 tgcaacgttc tacaacaaat ttgagttgat tgtcgagggc aagaaatggg ctttcgtaac
     5821 atctgaccgc tgttttactg gtcgatttag taaagaaggg aaaccgattc tagaggatgt
     5881 ccaatctggt gaagccgtgt attcaacaga gaaacaagtg tttcttatgg caggtgattc
     5941 aaaaagcctg ggatctttga ttcaagatgg tgaagcccac ttcgtggtta ccgatccccc
     6001 ttatgctagt aatgtaaact attcagaact agccgatttc ttttatgttt ggctacgtct
     6061 cagcttgtcg aagacttatc cttactttgc accagaatta actcctaaag tccaggaaat
     6121 aatcgtcaat ccgacgcgag gacatacaga agaggatttt aagttagggc ttcaggctgt
     6181 atttgaggaa tgtagacgta aattgttgca aaatggcctt ctacttttca cgtttcatca
     6241 ttctgaaggc aaagcctggg aagctctctt agaggctgtt tgcgaagccg gttttgaaat
     6301 tgacagtgtc tatcctattc aaggtgaagc agagacgtca cttcatttgc ttgagaaaga
     6361 ggcaattgcc tatgatctaa tccatgtttg tcgtaagcga aaagttgaac aagaaattaa
     6421 aaagcgttct tgggcgggga taagacagga aattcggcga agatcacggg aggaaattaa
     6481 agcgattgag gctggcaggt atggtcaaga gctttcccca gctgatgtaa acatcgtcct
     6541 tattggtaag tgcctcgaac tttacagccg tcactacggg gctgtcgttg accatgaagg
     6601 aaaagaagta accttgaaac aggcactcga ggaaattcgt atgatggttg accagctact
     6661 aacaagggaa cagcctttac catcagaact tagtgatatt gaccctgaat cttatgtata
     6721 tctaacctct ctctgtgatc gtaaagaagt taagagcgat gatgtgcata aagccaccag
     6781 aggaatactg gaacctgagt ccctgattaa ggcgggaatt atgataaagg gtagagcggg
     6841 tcgtggcaga acccatgagg taaaacaacc gtccgaacgt tttaactctc ttcttgagaa
     6901 gtttaaagaa actcccttac cacagcaaga tctttttgga gaaatcgaag ctccaaaaat
     6961 gaaaggaaac atatacttta ttgactatat tcactttctt atggctcttg ttgaaggggg
     7021 agagaatatt atcccctggt tggaaggttt cagaggggag acaccaagat taagagccgc
     7081 ttgtgagtac ttaatggcga ggaataggag ttttactcct actcttaaga aaattattga
     7141 tctaatagag gtagggccac tttttcagac aaggtgatta aatgcccgga taccaagtgg
     7201 tagagattgt aaatttacgg ccaaataagg ataatggaat tatgccatat gaaaataaaa
     7261 ttggcttttt agacttctta agggagattg ctcaagaacc gtcccctttt ccaaaatttc
     7321 accggcttca agtgagcgga ttggaagagg tactttatgc gggtcgtccc aaccatgaag
     7381 agttagctct caaaatacat aaacttctta ggtcagcagc acagaacctt gagaaaagac
     7441 taattgaagt ccagattgtt tttaagggaa aacttatgag aggagagaac ctttgggttg
     7501 agtacagaaa tacaaaactt ccgattggac atattttcgg gacacccata agacaagtgg
     7561 atgctaaagg aaatccattt ttcaggtcga attttaattt aactaatgga taatattgtt
     7621 ttgagtgagc aatgagtgag catattcaga aaacgatcaa tgcgggtgaa aaagtaaaag
     7681 cttatgggtt tgagcacctt ggaacaggcg aagttatccg tctgcgtgaa tcaggaaaaa
     7741 cctaccaggt tgatgttgta tttgactgtg ttgatggacg aagattagaa actatcccat
     7801 tagataggct tgagcgtgtg cctgatctat ttgaaaggat ttcgacaggg gacgtagatt
     7861 caccacttga tttctttttg aaacagcttg catatcaact accgcttaat aatgcaggag
     7921 gcgaactttc caactcaaga accgaccttt tacctcacca gattctctta acacgggata
     7981 ttgttgaatc ccagcggcgc agattcctta tcgcagatga agttgggctt gggaagacca
     8041 tagaaacagg gcttataata agggaattac ttgctaggaa ggacgcagag cgcattctta
     8101 taatttgccc tgcaggactc actagaaatt ggcaacaaga acttagagat tgttttaggc
     8161 tacactttga tatacttggt attgatttca atgatacaac gtctctttca tgggaaactc
     8221 atcatcttgt gattgcctca atagatacaa taaagcaaac aagaagaata gaaagattaa
     8281 agcacgcccc taaatggcac attattgtgt ttgatgaggc ccatcatctg agtcgtaaaa
     8341 gatatggaaa aaaaatagat tacacacaga attatcgact ggccgaggca ttaagaggtt
     8401 atacccggga tatgcttttt ctttcggcaa ccccacatca aggtgatgca tttcaatttt
     8461 ggtctctaat tcaacttctt gacgatcagt tatttgaatc tccagagagt atgatagacc
     8521 atagaggact tctcaaccgg gtaatgttcc gtagaatcaa gcgtgaagtt acagatgcag
     8581 aagggaatcc aatcttcatg cgtcgccatg tccatactca aacctttccc ttagcagcgc
     8641 gggaaagatg gttctacgat cagcttactg aatacctgcg tgagggttat ggggttgctg
     8701 gtataggaga ggaaagaact acaagtaagc aaagagctgt aggttttgtt atggctatat
     8761 tccagaagat gatgtcttcg agtcctcgtg ctattcgtca ggctctgaga cggaggcttt
     8821 tggtactgtt agcacgggaa cagatgactc tggaaaggaa aatccaagag gtccgtgcta
     8881 ctggcttggc agataagatg ttaaatatcc aggaagagat gaggggttta gcgataaaaa
     8941 ttaattctat atctacctca ctagaacaga tggctttagc cgatgcctat attgctaagg
     9001 tgaaacagcg gcttgctcga aaaggctatg ctggagagga aacaacgtcc tgggctcttg
     9061 attccgaaga agaagctgaa gatattatat atgccgaggc agtaatcccc aatgaaagcc
     9121 agaaggtgcg agaacttatc aagcttgccc cagaaggacc ggatcggaaa tttgatacac
     9181 tcataagggc tattgaacaa gttagaaggc tttcaccgca agagaaaatc atcgttttta
     9241 ctcaatatag agagacacta gaattcttaa atgaagagtt aagcaaatta tatggcgaag
     9301 aaaaagttgc taccctcaag ggaggtcctc ttgaagacaa aattgcggcc gtagaagctt
     9361 tttgggaacc agatggagca cagtttttga ttagtacttc tgccggaggg gagggaataa
     9421 atcttcaagt atgtaatatt atgtttaatt acgatctacc ctggaatcca atggcagtgg
     9481 aacaaaggat tggtcgcatt cataggtatg gacaaactga cactgtgcag gtttacaacc
     9541 tcgttgctga agatacagtg gaggaacgga tctatggtct gcttgaagaa aaattattgg
     9601 acattgccaa gaccatcggg aaacttgatc cagtgacagg tcaggttgta gaagacttta
     9661 gatcagaaat tctcggtttc ttaggaagct ctcccaacta tcaagacctt tacaagaaag
     9721 ctttaataga caaagactat caaagaaccg agcaagagat agtcgaggca attgagaaag
     9781 cacaaaaagc ctcagaagcc cttcgttatc tttcacaaga ccttgagtct tttaattttg
     9841 ggcgatacag gaatatgaaa gggcctttca cccttgatga ccttggactc ttta
//
LOCUS       DATDRL010000262         2441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3194883, whole genome shotgun sequence.
ACCESSION   DATDRL010000262 DATDRL010000000
VERSION     DATDRL010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3194883"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..626
                     /locus_tag="VI935_08830"
     CDS             336..626
                     /locus_tag="VI935_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018624064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HLE25739.1"
                     /translation="MYTYYVYIMASKRNGTLYIGITNDLIRRAYEHRNDLVEGFTKKY
                     GVRKLVYYGRTNDIYSAIQREKRFKKWNRQWKIDLIEKVNPDWKDLHDDLAK"
     gene            complement(756..1217)
                     /locus_tag="VI935_08835"
     CDS             complement(756..1217)
                     /locus_tag="VI935_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25740.1"
                     /translation="MTEPEEKKDKFQPRIYIDKEGNWYQDGIPILHRWTYLYNNKLLR
                     RDEEGRYYVDEGSGRVYAYVEDTPFVVKMIDKRQDGFFIILNDETEEKLDFNLLWMNE
                     ENIPYTKVKNGEFEARFSRPAYYEFTKYLKQEGDKFYIELDGNKYDLKAKM"
     gene            complement(1354..1767)
                     /locus_tag="VI935_08840"
     CDS             complement(1354..1767)
                     /locus_tag="VI935_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HLE25741.1"
                     /translation="MKSHTEYLWFNTKNRKEIIHITDKLREIVKKSSVEEGFVLVSAM
                     HITAAVYVNDLEEGLFEDIWEWLEKLAPYRKDYKHHGTGEDNGDAHLKNLIMHHQVIL
                     PITKGDLDLGPWQRVFYAEFDGQRRKRVIIKVIGE"
     gene            complement(1881..2138)
                     /locus_tag="VI935_08845"
     CDS             complement(1881..2138)
                     /locus_tag="VI935_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25742.1"
                     /translation="MKEVTKLDIYDEYKKAQEELWKGYQEIRDEFIKGYLAKKLEMTY
                     EEFKKMEKSLRDKYIDGRRQLLEKYFRESAISNQQSAISDQ"
BASE COUNT          677 a          503 c          403 g          858 t
ORIGIN      
        1 gatacttaag atcatataca gcatgatgtg ttctttttac tgggctcata cccagatttt
       61 atattcttta ttgcccaaag ggcaaacctg ttttcatccc cgtagtagaa ctacggggta
      121 ttcaacaggt attttaataa aaggctgtca ttctctcatg ttacccgata agagcattcg
      181 ggtacgggct taaccatgag ttcactattc ctttgtcatt cccaaacgcc tttattggga
      241 atccaaaaag tatggatacc cgataaaaac attcgggtat gacaagggta gcgataccca
      301 tataatgcat tcgggcatga cagtggtggg ggattatgta tacctattac gtttacataa
      361 tggcaagcaa aaggaacggt acgttatata ttggtattac taacgacctc attagaaggg
      421 cttatgaaca cagaaatgat ctagttgaag gatttacaaa gaaatatggt gtacgtaaat
      481 tggtctatta tggacggact aatgacatat acagcgcaat ccaaagggaa aagaggttta
      541 agaaatggaa ccgacaatgg aaaatcgatc tcattgaaaa agtcaatccg gattggaagg
      601 atttgcatga tgacctagca aaataagtgt actttcattt tacattttac actagagagc
      661 ttcatcatga gagccataaa tattatgtaa catgagttat agaaatgtct tgttcgctaa
      721 ctcgttttcg tgtttattgt tagttaagtg gttaattaca tttttgcttt taagtcatac
      781 ttattcccat ctaattctat ataaaactta tccccttcct gcttcaagta cttggtaaac
      841 tcataatagg ctggtcttga gaacctggct tcgaattctc cattttttac ctttgtgtaa
      901 ggtatgtttt cttcgttcat ccacagtaaa ttgaaatcga gcttctcttc agtttcatcg
      961 tttaatatta taaagaaccc atcttggcgt ttatcaatca ttttcactac aaaaggggta
     1021 tcctccacat atgcgtaaac cctcccactt ccttcatcta cgtagtatct tccttcctca
     1081 tcacgtctta gtagtttatt attgtaaaga taagtccatc tgtgtagaat tggaatccca
     1141 tcctgatacc aatttccttc tttatcaatg tatattctcg gctgaaattt atcttttttt
     1201 tcttcaggtt ctgtcataat atttttatta tatctcactc aagttggttg tttcaattta
     1261 tccgaaatcc gcactccgca atctaaaatt ggcacatcag cagtctgcaa tctgtaatcg
     1321 gcgatgacag agcatcaaaa cctctgtggc gaactactcc cctatcacct taataatcac
     1381 cctttttctc ctttgtccat cgaattcagc atagaagact ctctgccagg gacccaggtc
     1441 taaatcccct tttgtgatgg gaagtatcac ttgatgatgc attattagat ttttaagatg
     1501 ggcatcaccg ttgtcttctc ctgttccgtg gtgtttatag tcttttctat atggtgctag
     1561 tttttctagc cattcccaaa tgtcttcaaa aagtccttct tccaggtcat ttacatatac
     1621 ggcagccgtt atatgcatcg cagaaactag aacgaatccc tcttcgacgc tgcttttctt
     1681 cacgatctcc cttaacttat cggttatgtg gattatttct ttcctgttct tagtattaaa
     1741 ccaaagatat tctgtgtggc ttttcatggt tttcctcttt tgtaggggcg aaccactgtg
     1801 ttcgcccgtt ttttctgtgt gttttagggc agacacgagg gtcgttcgac tgaactcacg
     1861 acgaagtcag taaatgcctt ttactgatcg ctgatggctg actgctgatt actgatagct
     1921 gattccctaa aatacttttc aagcagttgc cttcttccat ctatgtattt atcccttaac
     1981 gatttctcca tctttttaaa ctcttcataa gtcatctcaa gcttttttgc aagatagcct
     2041 ttaatgaact catctcttat ctcctggtaa cctttccata gttcttcttg cgcttttttg
     2101 tattcatcat agatatcaag tttcgtaacc tctttcatta tttcatcttt cagataagta
     2161 tttgtatatt tgtttttagc ggacatcaat ggaggtattt gataagaaaa aacgatgata
     2221 aataaaaata tcatccaatt caatttagca tcctctcctg cttaaataat cctaaaactc
     2281 attataaaca ctaattatat gttttgaaat aattatttgc tcctttttcc ttaactccta
     2341 taccttctcc acaccctcct tatcacaatc aatgcgggaa ttaagagata aagtggaatt
     2401 gatgttgtag tgttaattga tgcaacagaa caaccaccac c
//
LOCUS       DATDRL010000263         2355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1985823, whole genome shotgun sequence.
ACCESSION   DATDRL010000263 DATDRL010000000
VERSION     DATDRL010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985823"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..740)
                     /locus_tag="VI935_08850"
     CDS             complement(90..740)
                     /locus_tag="VI935_08850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HLE25743.1"
                     /translation="MGNKLRFQSLGSIGDYVAVAYKLTKDTIKVTDGSIVAYVIVIFV
                     IILLRRFLPSFVLPIAAILFFSAPYLMKSQVIGLKWNLNGVLLGVVVSVIILSIYVVI
                     VNKPIKLGKISYTLLILQLFLVSLPEEVFFRGYLQEKIGNNLKGILIVSSLFAIGHFV
                     TLCLGGGYVGVGCMKTLLTFFPSIVMGYMYAKTGTLWGNVIFHFLANVVYEGTGGL"
     gene            1394..>2355
                     /locus_tag="VI935_08855"
     CDS             1394..>2355
                     /locus_tag="VI935_08855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLE25744.1"
                     /translation="MQQNTNIQSHIELPIAKTLLSVVVPAYNEEEVLPDFHKRISSVL
                     ENMSIEAEIVYVNDGSTDNTLAVMQGLQESDPRVAIVDLSRNFGKEIALTAGLDHASG
                     DAVVVIDADLQHPPELIPELVKHWMEGYDVVYAKRFSRSGEGVIKKATTFVFYRLIQM
                     VSRLKIPQDAGDYRLLSRRALESLKQLREQHRFMKGLFAWIGYPQKSVPYRPDPRYSG
                     QTKWNYWTLWNFALEGITSFSIAPLKLATYIGLVTAIVAFIYAGVIVYQTLAYGNPVA
                     GYPSLMVIILFLGGVQLITIGIIGEYLGRMFDETKQRPLYFLKG"
BASE COUNT          718 a          483 c          434 g          720 t
ORIGIN      
        1 cctaccctga tgttatccat gatgcctgtt agtataatac ttgcaaaaat cgtatcaagt
       61 aggggctgca tatatgcagc ccctacccat tacaatcccc ccgtaccctc ataaacaaca
      121 ttagcaagaa aatggaatat aacgtttccc cagagcgtac cagttttggc atacatataa
      181 cccataacga tagagggaaa aaatgtaaga agggttttca tgcaaccaac tccaacgtaa
      241 cccccaccca aacacaatgt aacaaaatgc ccaatggcaa acagtgaact gactattagt
      301 atgcccttta aattatttcc aattttctcc tgaaggtagc ctcgaaaaaa aacctcctca
      361 ggtagagaga caagaaaaag ttgcaagata agaagtgtat aagaaatttt accaagcttt
      421 ataggtttat ttacaataac tacgtaaata gaaagtataa ttactgatac gacaactcct
      481 aatagcactc cgtttagatt ccacttgaga ccaattacct gactcttcat caaataaggt
      541 gccgagaaaa acaaaatagc cgcaattggt agcacaaaac tgggaagaaa tcttctaagc
      601 agtatgatga caaatataac aatcacatag gctacaatag aaccatcggt gaccttgata
      661 gtatcttttg taagtttgta tgcgacagct acgtagtctc ctatagaacc taaggactga
      721 aaccgtagtt tatttcccat aagtcacatt aatcctcttt gatagacttt caatatcaaa
      781 tgtaatagag gtatctcgtc tgagggctac tttcataacc tacctcctta aaaattatct
      841 ctaacgtatc cgattaggca tcataagata ttctcattta attttatcct cttgttgatt
      901 tttattattt cggcattgta agcaaaactt accatcttgc tttctcttct tcatagacaa
      961 taataactaa gctatttatt ccgagacact tataaaaata aatccaattc ccaagaaagt
     1021 tgtgtacatt tagtcggttt atactatctt ggatctgagc gaggtgtttt atctccctta
     1081 tcgaaagatt tccttcattt atccgatttc taaacttatc taatataata accttttcat
     1141 agatcgattc tagtaattat agattaatat attcttattt aatcaactcc gttttaggtt
     1201 actcgtagtg gagaaatttg ttgacaagaa gcctcaaatt tgttaaaaaa tgattcatat
     1261 aaacatagga gtaaactatc attatccgtt attttctgtt agtttaattg tatatatttg
     1321 ggaaagtttt atggattgct ttagctttct actttgcagt tcataaattt gtaggtgttt
     1381 ggagaaaagt tgtatgcaac agaacactaa tattcaatcc catatagaac tccccattgc
     1441 taaaacactt ctctctgtag tggtgcctgc atataatgaa gaggaggtcc tgcccgattt
     1501 ccataaacgc atttcctcag tcttggaaaa tatgtcgata gaggcagaaa ttgtctacgt
     1561 taatgacgga agcaccgata ataccttggc agtaatgcaa ggtctgcaag aatctgatcc
     1621 acgagtcgcc atcgttgatt tgagccgtaa ctttggtaaa gagattgctt tgaccgctgg
     1681 acttgaccat gcaagtggtg atgcggtagt ggttattgac gcagatttgc agcatccacc
     1741 tgagctaatt ccagagttgg tcaagcattg gatggagggc tacgatgtgg tgtatgctaa
     1801 gcgcttttcg agaagtggag agggggtgat caaaaaggcc actacttttg tcttttatcg
     1861 cctgattcag atggtaagcc gcttaaagat tccgcaggac gctggcgact accgcttgct
     1921 tagccgtcgc gcccttgagt cactcaaaca gctacgggaa cagcaccgct tcatgaaagg
     1981 actgtttgca tggataggtt atccccaaaa atccgttcct tatcggcctg atccccgcta
     2041 ttctgggcaa accaaatgga attattggac tctttggaat tttgctctag agggaattac
     2101 ctctttcagc attgcccctc taaaactcgc cacctatatt ggcctcgtca ccgcaatcgt
     2161 ggccttcatt tatgccggcg ttattgtata ccaaacactt gcttatggga acccagtagc
     2221 aggttatccc tcacttatgg taattatact gttccttggt ggtgttcagc ttattactat
     2281 tggcattatc ggggaatatc tgggacgaat gtttgatgaa acaaagcaaa gaccgctcta
     2341 ctttttgaaa ggtta
//
LOCUS       DATDRL010000264         5794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1034234, whole genome shotgun sequence.
ACCESSION   DATDRL010000264 DATDRL010000000
VERSION     DATDRL010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5794
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034234"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1626
                     /locus_tag="VI935_08860"
     CDS             199..1626
                     /locus_tag="VI935_08860"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HLE25745.1"
                     /translation="MSQSPERREGDKAISKDVIASASARNDSLHELPKGWVWTKVGEI
                     IEKVSITGKKLKQSEYQEEGKLPVIDQGQTFVGGYTDKEELKVRCEPPVIVFGDHTKV
                     FKYVDFEFVAGADGVKVINPLEVFYPKLFYYFLNAIQLPDKGYARHFQFLEKSFIPLP
                     PLPEQQRIVAKIEELFTNLDAGVEALKKVKAQLKRYRQAVLKYAFEGKLTEKWREKHK
                     NELEPASLLLEQIREERKKNTKGSNVGATGRSPLPVDTSDLPELPEGWVWTKVGEIYD
                     IVGGGTPSTEVEEYWNGDIPWITSADIQGLKDIRPRRHINKKAIENSATNLVPEGSLI
                     VVTRVGLGKIALINNPICFSQDSQALISSNLLMYPDYSLYYLSQAVQIFKYKHRGTTI
                     AGVTKKQLSELTFALPPLSEQHKIVEEIERRFSVADKIEKVVEGSLKQSERLRQSILK
                     KAFEGKLVPQNPSDEPAEKLLERIK"
     gene            1813..2274
                     /locus_tag="VI935_08865"
     CDS             1813..2274
                     /locus_tag="VI935_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014274081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLE25746.1"
                     /translation="MVDGVIVLNDFGKIVESVWSDLPKHYSDVRLDAFIIMPNHVHSI
                     IFIVGANVRAGFKPAPTTNKRHPLSELVRGFKTFSSRCVNEIRGLSGTPVWQRNYYEH
                     IIRNEKDLNEIREYIVNNPTKWEMDEENPRNIKVGAGFKPAPTGKFKQYGE"
     gene            2264..3712
                     /locus_tag="VI935_08870"
     CDS             2264..3712
                     /locus_tag="VI935_08870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HLE25747.1"
                     /translation="MANESSTIVQKLWNYCNVLRDDGVSYGDYVEQLTYLLFLKMADE
                     QTKAPFNRPSIIPKGFDWQSLLSKDGDALEVHYRHILETLGKEKGMLGVIFRKSQNKI
                     QDPAKLKRLVMLINDETWVGLDIDVKGEIYEGLLQKNAEDIKSGAGQYFTPRPLIKAI
                     VEVMRPEPGMTICDPACGTGGFLLVAHDYIVQNYKLDKEEKRKLKYETFKGKDIVDGV
                     VRLCVMNLYLHGIETTIETGDSLISDPGDRFDVVLTNPPFGKKSSITIFSGEGRVDKE
                     SLIYERQDFWATTSNKQLNFLQHVKTLLKINGKAAIVVPDNVLFEGGAGETVRRKLLA
                     ECDVHTLLRLPTGVFYAQGVKANVLFFDRKPASEKHWTEKLWIYDLRTNKNFTLKTSP
                     LRYEDLEDFVRCYNPENRFERQETERFKAFTYDELTKRDKLSLDIFWLKDESLEDSEN
                     LPDPDLLAKEIAENLESALEQFGSIYEDLERK"
     gene            3828..4043
                     /locus_tag="VI935_08875"
     CDS             3828..4043
                     /locus_tag="VI935_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25748.1"
                     /translation="MYLPGARFEVKLRIKLGDEVFEPGLKGTIIEGIPKMVGKAYIVK
                     FDDGRIAQIHIVIMNNQTKIITGENVG"
     gene            complement(4044..5306)
                     /gene="hisS"
                     /locus_tag="VI935_08880"
     CDS             complement(4044..5306)
                     /gene="hisS"
                     /locus_tag="VI935_08880"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HLE25749.1"
                     /translation="METRSIRGFNDILPDQVKRWQHIEEKARTIFEIHGYSEIRTPIV
                     EFTDIFARSIGTTTDIVEKEMYTFTDRDGSSITLRPEGTAGVVRSFIEHNLYAKSPIT
                     KLYYMGMMFRHERPQKGRYRGFYQIGAELFGSKEPLADAEIILMLWRFFEGIGISPFL
                     KLEISSLGDEKCRPFFKRELIKFFKPKRNELCEDCQKRLEQNPLRIFDCKKERCKEIA
                     EKAPSILDNLCDECKEHFDSVKESLENIGTPYVLNPRIVRGLDYYTRTVFEVTTEELG
                     AQNAVGAGGRYDALVEELGGPPTPAVGFALGMERMILLHEKMFPDGFQKEADAFIAHI
                     GSEARNRAFLLAYELRKNGVVIDTDYEDKSLKSQLKRADKLGVKYTIIIGEEELRRGV
                     VKVRDMRKSLEEEMSLEDVKKLKTKLKI"
BASE COUNT         1814 a         1095 c         1244 g         1641 t
ORIGIN      
        1 gcagggatgc gcgataaatt agttcatgaa tattttggaa ttcgacatga tgttgtttgg
       61 gaaactataa gcaagcggct acctaaggtt agacggttag ttgagactct attaaaaaag
      121 atggacgagg aaataaaaga aaaatgaggt tttgacggtg tggaggataa aaataaaaac
      181 ttcattgcga ggagcaaatt gagccaaagc ccagagcgaa gagaagggga caaagcaatc
      241 tcaaaggatg tgattgcttc ggcttcggct cgcaatgaca gcttgcacga acttcctaag
      301 ggatgggtct ggaccaaggt cggagaaata atagaaaaag tttctataac tggaaagaaa
      361 ctaaaacaaa gtgaatatca ggaagaaggt aagctacctg taatagacca aggtcaaact
      421 tttgtcggag gttacacaga taaagaagaa ctaaaggtgc gttgtgagcc accggtaata
      481 gtttttggcg accatacaaa agtttttaag tatgttgatt ttgagtttgt agctggtgct
      541 gatggtgtaa aggtaataaa tcctttggaa gtattttatc caaagttatt ttattacttt
      601 ttaaatgcaa tccaattacc agataagggc tatgcgaggc actttcaatt tttagaaaaa
      661 tcatttatcc ctctcccccc tctccctgaa cagcagcgca tcgttgccaa gattgaggaa
      721 ctttttacca atctggatgc gggtgttgag gcgttaaaga aggtaaaagc gcaactgaaa
      781 cgctaccgtc aggcggtttt gaagtatgca tttgaaggaa aactaacaga gaaatggcga
      841 gaaaaacata aaaacgaatt agaacccgcc tcgttgcttc tggaacagat tagggaagaa
      901 aggaaaaaga atacaaaagg gtcaaatgta ggggcgaccg gccggtcgcc cctacccgtg
      961 gacacctcag atttgcccga actgccggag ggatgggtgt ggacgaaggt tggggagata
     1021 tatgatattg taggaggtgg tacgccttca acagaggttg aagaatattg gaatggtgat
     1081 attccttgga taacaagcgc tgatatccaa ggtctaaagg atattagacc aagaagacac
     1141 attaataaaa aggctattga aaattctgct acaaatcttg ttcctgaagg aagtttgatt
     1201 gttgttacca gggtgggatt agggaagata gctttaatta ataatccgat atgttttagt
     1261 caagattcgc aagccttaat tagcagtaat ttgcttatgt atccagatta ctcgctctac
     1321 tatctttctc aagctgtcca aatatttaag tacaagcata gaggtacaac aatcgctgga
     1381 gtaactaaga agcaactctc agagctaaca tttgcacttc ctccactctc tgaacaacac
     1441 aaaatcgtcg aagaaatcga gcggcggttt tctgttgcgg ataagataga aaaggttgtg
     1501 gaaggtagcc tcaaacaatc agaaagatta cgccagagca ttttgaaaaa ggcgtttgaa
     1561 ggcaaattgg tgccacaaaa cccaagcgac gaaccggcgg agaagctatt agaacggata
     1621 aaataggggt ttgattaatc aaacccctac tacaacaaat ccgcgagcga tgcggaaaca
     1681 ccataaaaaa tgacgtacga tccggagaaa catcatcggc gttccattcg attaaaagat
     1741 tatgattatt cacaagcggg ggcgtatttc ctgacaatat gcacatataa tagagaatgc
     1801 actttgggtg atgtggtgga tggggttatt gtgttaaacg attttggtaa aattgttgaa
     1861 tctgtttggt cagatttacc gaaacattat tctgatgtta ggttagatgc gtttattata
     1921 atgccaaatc atgtccacag catcattttt atagtagggg caaatgtaag ggcgggtttc
     1981 aaacccgccc ctacaacgaa taagcgccat ccattatcgg aacttgtccg cgggtttaaa
     2041 acgttttcgt ctcgctgtgt taatgaaatt cgtggcttat caggtacacc tgtttggcaa
     2101 cgaaattatt acgaacatat aatacgaaat gaaaaagatt tgaatgaaat cagggaatac
     2161 atcgtgaaca atccaactaa atgggaaatg gatgaagaga atccgaggaa tatcaaagta
     2221 ggggcgggtt tcaaacccgc ccctacaggg aaatttaaac agtatggcga atgaatcttc
     2281 tacaatcgta caaaaactct ggaactactg caacgtcctt cgtgatgacg gcgtgagcta
     2341 cggcgattat gttgagcagt tgacctacct attgttcttg aagatggcgg acgagcagac
     2401 aaaagcgcct ttcaataggc cctcgataat acccaaaggc tttgactggc agagtcttct
     2461 ttctaaggac ggagatgcac tggaagtcca ttaccgccat atcctagaga ctctgggcaa
     2521 ggaaaaggga atgcttggag tcatcttcag aaaatcacaa aacaagattc aggacccggc
     2581 aaagctcaaa cgcctggtta tgctgattaa tgatgaaacc tgggttggtt tagatattga
     2641 tgtaaaggga gaaatttacg aagggctttt acagaagaac gcagaggata taaaaagcgg
     2701 tgcaggacag tacttcactc ctagacctct cattaaggct attgtggaag ttatgagacc
     2761 tgaacctggg atgacaatct gcgaccctgc atgcggtact ggtggatttt tactcgttgc
     2821 tcatgactac attgtccaaa actacaaact tgataaggaa gaaaagagaa agctgaaata
     2881 cgaaacattc aaaggaaaag acattgtaga tggagtagta aggttatgtg ttatgaatct
     2941 atatttgcac ggaatagaaa caaccattga aactggagat agtctaattt ctgacccagg
     3001 cgaccgtttc gatgtggtgc ttactaatcc tccatttggc aaaaaaagca gcattaccat
     3061 attcagtggt gagggaaggg ttgacaaaga atccttaatt tacgaacgtc aggacttctg
     3121 ggcaaccact tcaaacaagc aacttaactt tctacagcat gtaaaaacac ttctgaagat
     3181 taatggaaaa gcggcaattg tggtgcctga caacgttctc tttgaaggtg gagcaggaga
     3241 aactgtaaga agaaaactcc ttgccgagtg cgatgttcat acacttttaa gacttcctac
     3301 cggtgtattt tatgcccagg gtgtaaaggc aaatgtcctt ttctttgata gaaaaccagc
     3361 aagtgagaag cactggaccg agaaactctg gatatatgac ctaagaacaa acaagaactt
     3421 tacactaaaa actagcccgt tgcggtatga agaccttgag gactttgtca gatgttacaa
     3481 tccagaaaac cgattcgaga gacaggagac tgaacggttt aaagcattca cttacgatga
     3541 acttactaaa cgggataaat taagtcttga tatattctgg cttaaggatg aaagccttga
     3601 ggattctgaa aatcttccgg acccggactt gcttgcaaaa gagatagctg aaaatcttga
     3661 atcggctttg gaacagttcg gtagcatcta cgaggatttg gagaggaaat aatatttata
     3721 tagaattact ctcttctaag aatcggtctt catctgtggc aacgtgttga tttaactcag
     3781 tgcaaactat tccatgagct ttagctttgc tgtaggaggg ataaacagtg tatttaccag
     3841 gtgcaagatt tgaagtaaaa ctgaggatca aattaggtga tgaggtattt gaaccaggct
     3901 taaagggaac cattattgag ggtataccaa agatggtggg aaaggcttat atagtaaaat
     3961 ttgatgatgg taggattgct caaatccata ttgtaataat gaacaatcaa accaaaataa
     4021 ttacaggtga aaatgtggga tagttaaatc tttaacttag ttttaagctt ttttacatcc
     4081 tctaagctca tttcctcctc aaggcttttt ctcatatctc ttaccttgac tacaccccgt
     4141 cttagttctt cttctccaat gattatcgta tacttgacgc ctaatttatc cgctcgctta
     4201 agctggcttt taaggctttt atcttcatag tccgtatcaa taactacacc gtttttccta
     4261 agctcataag caagaagaaa agccctattc cgtgcttcac ttcctatgtg tgctataaag
     4321 gcatccgcct ccttctgaaa tccatcagga aacatcttct catgaagtag tatcattctt
     4381 tccatgccga gagcaaagcc aacggcaggt gtaggtggac cgcctagttc ttcaacaagc
     4441 gcgtcgtatc tcccgcccgc tcccacggca ttctgagccc caagctcttc ggttgtcacc
     4501 tcaaaaactg ttcttgtgta gtaatccagc cctcttacaa ttcgaggatt gagaacatat
     4561 ggagtgccga tattttctaa agattcttta actgaatcaa aatgttcttt acattcatcg
     4621 cataagttat ccaagataga gggggctttt tcagcaatct ccttgcatcg ttctttctta
     4681 cagtcaaaga ttcttagtgg gttctgttca agccttttct gacaatcctc gcagagttca
     4741 ttcctttttg gtttaaaaaa ttttatgagt tctcgcttga aaaaaggacg gcatttttcg
     4801 tctccaagag agcttatctc aagcttgaga aaagggctta ttccaattcc ctcaaaaaac
     4861 ctccaaagca ttaatattat ttctgcatct gcaagaggtt ccttagaacc aaaaagctct
     4921 gccccgattt gataaaaacc cctatatcgt cccttctgtg gtctttcgtg cctgaacatc
     4981 atacccatgt aatagagttt tgtgatagga gacttggcgt acagattgtg ttctatgaat
     5041 gatcttacaa ctccggcagt gccttctggc ctaagggtga tggatgaacc gtctctgtct
     5101 gtaaacgtat acatctcctt ttcaactatg tcggttgttg taccaatgct tcttgcaaaa
     5161 atatccgtaa actcaacgat tggcgtcctt atttccgagt aaccgtgaat ttcaaatata
     5221 gttctagcct tttcctcaat atgctgccat ctcttaactt gatcaggcag tatatcgtta
     5281 aaacctctta tcgatctcgt ttccatgtta cttggattct atcacaggga ttaggaattt
     5341 ggtattacga attgggaatt aggaattagt tatttattgt tcaatgcgtg aagatttata
     5401 ttaataaagc ctaacactaa atgctagtag ctaattggag agactaatcc aattattaac
     5461 tgttaggtta tttgtattcc aatttcaagt aatcaataaa agcatcgagt acatcatgca
     5521 cggttaatat gcctacaagc cctcctttat ttgaaacaac aggaagagca ttgaattttt
     5581 tctttcttaa ttaagcatat agattcaagg gtgccatctt cttttaccat catcccattt
     5641 cgcatccttt ttagggttat atattttatc cataaaatta gccttttggt tggctcaaaa
     5701 gatagactat tttagctcca accagtcgcc attctcaaac tggtaagtct gattcagtat
     5761 tgattcagga ggaatttcct cagcgcttat aaca
//
LOCUS       DATDRL010000265         3670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2480915, whole genome shotgun sequence.
ACCESSION   DATDRL010000265 DATDRL010000000
VERSION     DATDRL010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2480915"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VI935_08885"
     CDS             complement(<1..875)
                     /locus_tag="VI935_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HLE25750.1"
                     /translation="MLRLNDIVEKVNSYLPEADVEVIKRAYVFSAKVHGGQKRSSGES
                     YLGHPLEVAGILADLRLDLSSVVTGLLHDTVEDTLATLDEIEALFGKEVALLVDGVTK
                     ISKLPFTSKIEEQAESFRKLFLATAKDIRVVLVKLADRLHNMRTLEYLPEDRQKRISK
                     ETLEIYSPLAHRLGIYWIRTELEDLSFRFLSPEEYENISKLIAKKKKEWEKYVESVQG
                     ILEKQLIEFGIKTEITGRFKHIYAIYNKMQTQNLEFDQVHDVIAFRIITSSLRECYEA
                     LGAVHAAWKPVPGRF"
     gene            complement(1001..1600)
                     /gene="gmk"
                     /locus_tag="VI935_08890"
     CDS             complement(1001..1600)
                     /gene="gmk"
                     /locus_tag="VI935_08890"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HLE25751.1"
                     /translation="MRETIIFVVSGPSGSGKTTLTRRVIKMVENLRFSVSFTTRLPRP
                     DEVDGIDYRFISEKEFDEMVKNKRFAEWAIVHGYHYGTPIEELERMRSLGVDLLLDID
                     AQGAKSIRNLYGSGVYIFVTTSSLEVLKERLIRRHGEGMVEIARRIEDSRNEMDEIDS
                     YDYIIINDSVDEAVMKLKAVIVAERCKRERVLETLKKSF"
     gene            1843..2727
                     /gene="metF"
                     /locus_tag="VI935_08895"
     CDS             1843..2727
                     /gene="metF"
                     /locus_tag="VI935_08895"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HLE25752.1"
                     /translation="MRFSEYFKDGRRPIISFEIFPPKTDKGMEPLLEIIPELIALGPH
                     YITVTYGAMGSTQRRTLEIASLIKNRYGMETACHLTCVGASRIELNEILEQITNAGIR
                     NIVALRGDPPMGEEAFIPPQNGYSHANQLVEHIRRFEKERGRGPLGIAVAGYPEKHIE
                     APNMEIDIANLKQKVEAGGDIIITQLFFDNNFFFEFVERVRAAGITVPIIPGLMPILS
                     VKQIQKITSMCGSNIPRELQLELNEAADDDEKASEIGIRQCIRQARELLQHGVPGIHF
                     YVLNKSTHIRKIMETLRS"
     gene            2826..3509
                     /gene="ubiE"
                     /locus_tag="VI935_08900"
     CDS             2826..3509
                     /gene="ubiE"
                     /locus_tag="VI935_08900"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HLE25753.1"
                     /translation="MPETKSLFNRVAEHYDLLNTLLSFGMDRSWRRRLAEEITSSNLV
                     LDIATGTADVAIEVVKKWNGCFAIGIDPSKQMLDLAHTKLETARVKKKVALVEGFAED
                     LPFKNGTFDAITIAFGIRNTIDPLISLKEMNRVLKPSGRAGILEFALPRNRLFAPLYM
                     SYIRNMFPFLGSFFGKKNEYQYLVDSIPTFPQRDGFTRLMEEAGFSVIKPIELTMGTV
                     IIYVGVKDD"
BASE COUNT         1108 a          775 c          673 g         1114 t
ORIGIN      
        1 ttgaatcttc ctggaactgg tttccaagct gcatgaactg caccaagagc ctcatagcat
       61 tctctaagtg agcttgtaat aatcctgaag gctataacat catgcacctg atcgaactcg
      121 agattctgtg tttgcatctt gttgtaaatt gcataaatat gcttaaatct gccagttatt
      181 tctgtcttta taccaaactc tattagttgc ttctcaagta tgccttgtac cgactcaacg
      241 tatttctccc actccttttt cttttttgca attagttttg aaatattttc atactcctcg
      301 ggacttagaa aacgaaaaga cagatcctca agctctgttc ttatccagta aataccaagc
      361 ctatgtgcaa gaggagaata aatttcaagt gtttccttag aaattctctt ctgacgatcc
      421 tcaggaaggt attctaatgt gcgcatgtta tgaagcctgt ctgcaagctt aacaagaaca
      481 acccttatat cctttgccgt tgcaagaaat agtttcctga aactctctgc ctgttcctcg
      541 atctttgaag tgaatggtag cttgcttatt tttgtaacac cgtcaacaag aagtgcaacc
      601 tccttaccaa aaagtgcttc aatctcatca agggttgcaa gtgtatcctc cactgtatcg
      661 tgaagaagcc ctgttactac agatgacaaa tctagcctca aatccgcaag tatccccgct
      721 acctcaagag gatggcctag gtaagattct cctgaacttc ttttttgtcc tccatgaacc
      781 tttgcagaga agacataagc cctcttaata acctctacat cggcctcagg aaggtatgag
      841 ttaacttttt caacgatgtc gtttaatctc aacatgattt tctcataact tagctactaa
      901 taaacacgaa tttacacgaa taaaatgata catgaatttt tacgtgcatc ctgcatcatg
      961 tatcctgaat cttgcatcct gtatcttgca tcgtgcatct tcaaaagctc tttttgagcg
     1021 tttctaacac cctttctctc ttgcacctct cagcaactat aacagctttt agtttcatta
     1081 ctgcctcatc cactgagtca ttaataataa tgtaatcata agaatcgatt tcatccatct
     1141 cattccttga atcctcgatc ctccttgcaa tttcaaccat tccttcacca tgtcttctga
     1201 taagccgctc ttttaaaact tcaagtgatg atgtggtaac gaaaatgtaa acacccgaac
     1261 catacaaatt tctaatgctt tttgcacctt gagcatctat gtctagaagg agatcaacac
     1321 ctaaggacct cattctttca agctcctcga ttggagttcc atagtggtaa ccatggacta
     1381 tagcccattc agcaaacctc ttatttttaa ccatctcatc gaattccttc tcagatataa
     1441 atctataatc tattccgtca acctcatctg gtcttggtag tctagtcgta aaggaaacag
     1501 aaaatctaag attttcaacc atcttaatca cacgccttgt aagcgtcgtt tttccacttc
     1561 ctgatggacc agaaactaca aagatgattg tttctcgcat gatttcaaag ggttactact
     1621 actttttata agtttaggat acctttacac aatttgcaat tggaagaaat ttgttaggac
     1681 ctcatcttat ttgacaatat tccctaaaaa gtgaaaattt atgacttgtg tgctctttta
     1741 taatgctcct attttcttca aaaaatcgtt gatttattat cttaattcga agtcacgcaa
     1801 taaggataca aatgttttaa cttattaaaa tcatttaagc ttatgcgctt ctcagaatat
     1861 ttcaaagatg gcagaagacc gataatatcc tttgagattt ttccgcctaa gactgacaag
     1921 gggatggaac ccctcctaga aattattcct gaactaatcg ctttaggtcc acattacatc
     1981 acagtgacat acggagcgat gggttctaca cagcgtagga cactcgaaat cgcatctctt
     2041 ataaaaaacc gctacggtat ggaaaccgca tgccatctca catgtgttgg agcatcacgt
     2101 attgagctta atgaaatcct ggagcagatt accaatgctg gcataagaaa tatagttgca
     2161 ctacgcggtg atcctcctat gggtgaggag gcctttattc cgcctcaaaa cggatactca
     2221 catgcaaatc aattagttga gcacatccgg cgtttcgaaa aggagagggg tcgaggacct
     2281 ttgggtattg cagttgcggg ttatccagaa aaacatatcg aagccccgaa catggaaata
     2341 gacatcgcta acctaaagca gaaggttgaa gcaggtggcg acattataat tacccaactc
     2401 ttttttgata ataacttctt cttcgagttc gttgaaaggg tgcgtgcggc tggtataact
     2461 gtcccaatca ttccaggttt aatgcctata ctttctgtta aacagataca aaaaattacc
     2521 tctatgtgcg gctctaatat accccgtgag ttacagttgg aattaaacga agcggctgat
     2581 gatgatgaaa aggctagtga aatcggaata cgtcaatgta tccgccaggc aagagagctt
     2641 cttcagcatg gtgtgcctgg tattcatttc tatgtactaa ataaatccac acacattcgc
     2701 aagatcatgg agaccctgcg gagttagttc gtacgagtgc ctggatcgtg attcgtgatt
     2761 caacttctta ataaacacag ccaaacgaag cacgagcccc gattcacaaa tcacgagttg
     2821 ataatatgcc tgaaacaaaa tcacttttta atagagttgc agaacactat gacctgctaa
     2881 ataccttgct cagctttggt atggatagat cctggagaag gagactagct gaggagataa
     2941 caagttcaaa cctcgtgctt gacatagcta caggaaccgc agatgtagcg attgaggttg
     3001 taaagaagtg gaatggatgt tttgcaatag gcattgatcc gagtaagcaa atgcttgatc
     3061 ttgctcatac taaacttgaa acagccagag ttaaaaagaa agttgcttta gtcgagggat
     3121 tcgctgagga tttacctttt aaaaatggga cctttgatgc gataacaatt gcctttggaa
     3181 tcagaaatac tattgatccg cttatatcac tcaaagaaat gaatagagtt ctaaaaccca
     3241 gtgggagagc aggaattcta gagtttgctt tacctcgaaa taggcttttt gcacctctat
     3301 atatgtctta cataagaaac atgtttccat ttctcggatc gttttttgga aagaaaaacg
     3361 aatatcaata tctagtagac tctattccta cattccctca aagggatggt ttcactaggc
     3421 ttatggaaga agctggattt tcagtgataa aaccaataga gcttacaatg ggaacggtaa
     3481 ttatttacgt tggagttaaa gatgattagt ttaccgtaaa ccaattcata aattttttca
     3541 atgaagctcc ctgcggcaag ccgcagggta tcagcataaa aaagatagtt tctttggtca
     3601 ttgcaatgac agcacgaaaa tgtcattgcg aggaacaaag tgacgaagca atctagatac
     3661 gggattgctt
//
LOCUS       DATDRL010000266         2163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3479209, whole genome shotgun sequence.
ACCESSION   DATDRL010000266 DATDRL010000000
VERSION     DATDRL010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3479209"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VI935_08905"
     CDS             complement(<1..1136)
                     /locus_tag="VI935_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25754.1"
                     /translation="MTTIYRYLKWDGLQGEFKLEPQELFKEFSNFLMEGWTTDEAFEW
                     IMKQGVRGENIKVMGVDELRSELSKYKEMKFQTFNLRSSLSEIKDELDEIVEMEIDTL
                     KENLSRISPEYQRRKNFLRSLPQKLSTAIEKLSGYDFMDKEAKERFEEFKQKLESIKR
                     VENFIHRYSEKFRGKTSLGFQETLDLIEEFERIEQLEHNLMSGNFEVVRLEDLKKMAS
                     DEGYNSFILLRDFKSTLEKSGLVRIRGQYIELTPKGIRKTGEHALGDIFSSIKKNQFG
                     EHETTQRGFGTLKPEETKEYEYGDPFNLNVVGTLKNSLLRETKGSPIKVAPEDFEVYD
                     MEYHTQATTAILLDLSWSMSFQGRFPAAKRVALALDHLIRTHYP"
     gene            complement(1249..>2163)
                     /locus_tag="VI935_08910"
     CDS             complement(1249..>2163)
                     /locus_tag="VI935_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HLE25755.1"
                     /translation="DELTIHFGLIPRSNRGIFAINELPDLVEKVQVGLLNIMEEKDVQ
                     IKGYKLRLPLDILIVATANPEDYTNRGRIITPLKDRFQAQIRTHYPSTRDEEIKITEQ
                     EARVLKREGHTVKVPKFIKEIIAEITFQARGSHEINQRSGVSVRTTITNYESIIAAAE
                     KRAIKMEEREVSPRITDFPAIVPSTVGKIELEYLGEDTTEASVVEKLIKRAIKIVFDS
                     YFTSLSSFSELLESFEQGYVEVSDTMSSDEYLVGIKEVKGLKKCIETLGIDQTPGQIA
                     SAIEFILEGLHLSNKLNKEKVRGRIIYK"
BASE COUNT          546 a          468 c          394 g          755 t
ORIGIN      
        1 cttggatagt gtgttcttat taaatgatca agagcaaggg ctacgcgctt tgcagccgga
       61 aaccttccct gaaagctcat ggaccaactg agatctagta gaatagcggt tgtggcttgt
      121 gtatgatatt ccatatcata gacctcgaaa tcctcaggag cgactttgat ggggcttccc
      181 tttgtttcgc gaaggaggga gttttttagt gttccaacta catttaaatt aaacggatct
      241 ccgtattcat actctttggt ttcttctggt tttagcgtgc caaagccacg ctgagttgtt
      301 tcgtgttcac caaattgatt tttcttaatg gatgagaaaa tatcccctag tgcatgctca
      361 ccggtttttc taataccctt aggagtgagt tcgatatact gaccacgtat cctaacaagt
      421 ccagattttt ctagcgttga tttgaaatct ctaaggagga tgaaagaatt atagccttca
      481 tcacttgcca tcttcttaag gtcttcaagc ctcacaacct caaagtttcc tgacataaga
      541 ttatgttcaa gctgttctat tctttcaaac tcttcgataa ggtctaaggt ttcctgaaac
      601 ccaagtgagg ttttacccct gaatttttca ctgtatcggt gtataaaatt ttcaaccctt
      661 tttattgatt ctagcttctg tttgaactct tcaaacctct ccttagcttc cttatccata
      721 aaatcgtagc ccgatagctt ctcaatggcc gtacttagtt tttgtgggag gcttctaaga
      781 aagttttttc ttctttgata ttcgggtgat attctagaaa gattttcttt taaggtgtca
      841 atttccatct cgacaatctc atccagttca tccttgattt cagatagaga tgaacggagg
      901 ttaaatgttt gaaacttcat ttccttatat ttagagagct cagagcgcag ttcgtcaacc
      961 cccataacct ttatgttttc accacgtaca ccctgtttca taatccactc aaaggcttcg
     1021 tcagttgtcc atccctccat aagaaagttg gagaattcct taaaaagctc ctgaggttct
     1081 aacttaaact caccctgcaa tccatcccat tttaggtatc tatagattgt tgtcatgtaa
     1141 ctgtaagtag gaactagacg tgagggcgta aatcgtgagg cgtaaagggt taggcgattt
     1201 acccctcacg ctctcacacc ttacggtgtt tgcccaattc ctaattcctc atttgtatat
     1261 tattctccca cgaacctttt ctttgttgag tttgttgctt aaatgcaagc cttctagtat
     1321 aaattcaatt gctgatgcaa tttgacctgg ggtttgatct atgccaaggg tctcaataca
     1381 ttttttaaga cctttaacct cttttattcc cacaaggtat tcatcagatg acatagtatc
     1441 tgaaacctcg acgtagcctt gctcaaagct ctcaaggagt tcggaaaagc tgctcagaga
     1501 agtgaaatat gaatcaaaga ctatctttat cgcccgcttt attaactttt ctaccacgct
     1561 tgcttcagtt gtatcttcac ctagatattc aagttctatc tttcctacag ttgaggggac
     1621 gattgcagga aaatctgtta ttcttggcga aacctcgcgt tcttccattt ttattgccct
     1681 tttttctgcg gctgcgatta tgctttcata atttgtaatc gttgttctaa cgcttacacc
     1741 agaacgctgg tttatctcat gtgaccctct tgcctgaaag gttatttcag caattatttc
     1801 ctttatgaac ttaggcactt tgacagtatg gccttctctc tttaaaaccc ttgcctcttg
     1861 ttcagtaatt tttatttctt cgtcacgtgt tgaagggtaa tgcgttctaa tctgtgcctg
     1921 aaaacggtct ttaagcgggg ttattattct accacggttc gtgtagtctt ctgggttggc
     1981 ggtggcaact attaaaatat ccagaggaag gcgtaacttg taacctttta tttgtacatc
     2041 cttttcttcc attatattta aaagcccaac ctggaccttc tctacaagat caggaagttc
     2101 atttattgca aaaatacctc tgttactcct aggaataagt ccaaagtgga ttgtaagttc
     2161 atc
//
LOCUS       DATDRL010000267         4473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3457754, whole genome shotgun sequence.
ACCESSION   DATDRL010000267 DATDRL010000000
VERSION     DATDRL010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3457754"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="VI935_08915"
     CDS             complement(<1..1539)
                     /locus_tag="VI935_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HLE25756.1"
                     /translation="MKISELLDKNTIKLDLKAKKKAQAIQEVADILYKAKKIKNPQNI
                     VAVLLEREKVSPSTFENGVAIPHATIEGIKEVVSCLAISRSGIDFESPGKRPVRVIFL
                     FLSPFEESETHLQILSKAEGLLQNKKLCDALIASNSKEKLINEIINAERMGWDTYINL
                     SKEDVLLELGTTERGLSREEAIRRLQRYGSNLLEKIRRKPLILRFSANLTNLLAILLW
                     VAGILAFIANLPELAWAILVVIFINAAFSFWQEYKAEKGIEALRELIPSYSRVLRDGK
                     EERILTSDVVPGDVILLEEGDQVPADGRLFEAFDMRIDNSALTGESRPIYKIAEPVFD
                     GKNFLWTEIPNLVFGGTSVLSGSGRAIVIATGMHSEIGKIARLTQVIKEELSPLQREM
                     IKVTKVVSILAISMGILFFILGSYVAHLTTFESFIFAIGIIVANVPEGLLPTVSLALA
                     MAVQRMANRHVIIRRLSSVETLGCTTVICTDKTGTLTTNQISVVRLWFNNSLFEIEGS
                     GYDPS"
     gene            complement(2220..3017)
                     /locus_tag="VI935_08920"
     CDS             complement(2220..3017)
                     /locus_tag="VI935_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLE25757.1"
                     /translation="MRSVYVIAKREFLDAIRNRWFFFFAILFILLDSTVAYSGIIGGG
                     DNTFLKPAVSLLNLALILVPLMALIMGANSFTGAKESWELLLIQPISRKEAVLGKYLG
                     LGIAITSTLLLGFISAGLILILNIGSKELSHFLLLVFLSILLSLIFLSIASLVSIALG
                     EKAKSIGISFMLWFWFVIIYDFILVGLTLAYGELIIALLFLNPADLVRTTLLTSLGSA
                     ALVGPAGAVLHKTLGSVTGIIASLIVLLAWLLLPLLVALFIFERKDI"
     gene            complement(3019..3663)
                     /locus_tag="VI935_08925"
     CDS             complement(3019..3663)
                     /locus_tag="VI935_08925"
                     /inference="COORDINATES: protein motif:HMM:NF017394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HLE25758.1"
                     /translation="MKKSLSNTRRLILILAISSSFKRGFVTASDCRGNPCGCPGDRAG
                     ARPAPTVNLPNDRLLYALLKNRIGIKIAIFSFLLIIGCQSENTPVSINKGTDMCEYCR
                     MMIDDFHYSAEIVTKGKVHKFDDIGCMMAYAQSNDLSLDKAHFWVMDFDSATWIKGDE
                     AYYVLSPGIHTPMGYGIIAFKDQIKAKETEDKSKGEVKEFKSLFNIDWKQRPEH"
     gene            complement(3608..>4473)
                     /locus_tag="VI935_08930"
     CDS             complement(3608..>4473)
                     /locus_tag="VI935_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLE25759.1"
                     /translation="GVSLDIEKAETFAIVGPNGAGKTTLLNTIVGLVHPSGGKITVDN
                     IEVSKNPKEIKKRIGYLPQRISFHENLSVLEVIEFYASLRGINNNNLENVLSAVGILE
                     QKNKKVGVLSGGMLQRLALAQAIISDPPILILDEPTISLDPEGRIAFKRLIREEKRKG
                     KTILLSSHILSYIEELADRIAILVSGKVVALDTLGGLRTRLKMGSILEVVIENLSSQA
                     LSLVEDLGAKNATINGNRLRFFIPPEYKSKVIKTLVEYGFNVMDLWTEDSSLEEIFIK
                     YTEANSNSSNQ"
BASE COUNT         1332 a          944 c          823 g         1374 t
ORIGIN      
        1 acttggatca tatcctgatc cttcgatttc aaaaagacta ttgttaaacc aaagccttac
       61 tacgcttatc tggtttgtag taagagtgcc agtcttgtca gtacagataa cagttgtgca
      121 tccaagggtc tccacagatg atagccgcct tattattaca tgcctatttg ccattctctg
      181 cacagccata gccagagcca aacttactgt gggaagaagc ccctcaggaa cgttagctac
      241 aataatccct atcgcaaata taaaactctc gaaggttgta agatgggcaa cgtaactacc
      301 aaggataaag aacaggatcc ccatacttat agctaagata cttactacct ttgttacctt
      361 tatcatctcc ctctgaagcg ggcttaattc ttcttttata acttgagtta gtcgtgcaat
      421 tttgcctatc tcagagtgca tgccagtagc gataactatg gccctcccac ttcctgaaag
      481 aacactcgta cccccaaaaa caagattagg aatttcggtc cagaggaaat tctttccatc
      541 aaaaaccggc tcagcaatct tataaattgg tcgtgattcg cccgttagag cagagttatc
      601 gatccgcata tcaaaagctt caaatagtct tccatcagcc ggcacttgat ccccttcttc
      661 aagtagtatc acatccccag gaactacatc agaggttaaa atcctttcct ctttaccgtc
      721 tcttaaaaca cggctatagg atggaataag ctcccttaga gcttcgatcc ccttttcagc
      781 tttgtattcc tgccagaagg aaaaagcagc gtttataaaa ataacgacaa gtattgccca
      841 ggctagctca ggtaagttag ctataaaagc caggattccg gctacccaga gaagaattgc
      901 aaggagattg gtgagattag cagagaagcg aaggataagt ggttttcttc taattttctc
      961 aagtaaattt gatccgtacc tctggagtct tcttattgcc tcctccctag acaatcccct
     1021 ttcggttgta ccgagttcta gtagtacatc ttcttttgaa aggttaatat acgtgtccca
     1081 acccattctc tctgcattga tgatctcatt gattaatttt tccttgctat tactggcaat
     1141 aagtgcatcg cacagttttt tattttggag aagcccctct gcttttgaga gaatctgaag
     1201 atgggtttcg ctctcttcaa atggagataa aaagagaaat attactcgga ctggtctttt
     1261 acccggggat tcaaaatcta ttcctgacct agatattgcc aaacaggaaa caacctcttt
     1321 tatcccctcg attgtagcat gtggtattgc tactccgttt tcaaacgtag atggtgaaac
     1381 cttttctctt tctaaaagta cggcgactat attttggggg tttttaatct tctttgcctt
     1441 ataaagaata tcagccacct cttgtatcgc ttgcgccttt ttctttgcct ttagatctag
     1501 tttgattgta tttttatcta atagttcaga gatcttcatt atttcaatac tggtttaatt
     1561 ttgtctaagt tctattatat gaactataat tgtaaactat acgtatattg ttagatcgca
     1621 attgttatta atcgttattt attatttttg ttacctactc catatccaca tgcaaaatta
     1681 agaccgaaga aataaacgta gagataaact catgaacctg aggactgccc tattaatatc
     1741 ggtcgtttca gaaaagtatt tataaatgaa aatgtaaagg ggatcacttt aaccaatgtc
     1801 tatatcgaac atggatcaca tacaattgtt tatatagttg aggcggatga ctataataac
     1861 attgtaaagt tcctcgaacc agggatgtta aagtgttcct catccatctc accggtttcc
     1921 tctttgccag ttggtgaaat tttgaagaaa tagagttata ccaatcgaat acgaagagtc
     1981 catatgattc tttaaagtag gctcatgatc gccgttcatg gttcgacagt cctgtgctga
     2041 gactagacga agggttcacc atgaacagat aaccttagat accgtattta aatatcgttc
     2101 gtccgttcgg ctgagctcac gacgaagcct gagcacagtc gaagggcgtg tttgtcctct
     2161 taaacacttt gtggaatatg gcttatctaa aagcaatttg ttttagaaga gacattagat
     2221 caaatatctt ttctttcaaa aataaaaaga gctactagta gtggaagtaa cagccaagca
     2281 agaagaacta ttaaagaagc aattatacct gtaacacttc caagggtttt atgtagcacc
     2341 gctcctgcag gtcccaccag agctgcacta ccaagtgatg taagaagtgt agttcttaca
     2401 agatctgcag gatttaaaaa cagaagtgca atgattagct ctccgtaagc cagggttaaa
     2461 cccaccaaga taaaatcata gatgatcaca aaccaaaacc agagcataaa gctaatcccg
     2521 atagattttg ctttttctcc taaggctatc gataccagtg aggcaattga gagaaaaata
     2581 agagaaagca gaatagaaag aaaaacgagt aataaaaagt gtgatagctc cttagagcct
     2641 atgtttaaaa ttagaattag cccggcagaa ataaagccaa gaagaagagt tgatgttatg
     2701 gctattccga gaccgagata tttccctaac acagcttctt ttcggctaat gggctgtatt
     2761 aagagcaact cccacgattc tttagctcct gtaaaactat ttgctcccat tatgagagcc
     2821 ataaggggga caagaattag agcaaggttt agtaagctga cggctggttt taaaaaggta
     2881 ttatccccgc cacctatgat tccagaataa gctactgttg agtcaagaag tataaaaaga
     2941 atagcaaaaa agaaaaacca tctgtttctt atggcgtcta aaaactctct tttagcaatg
     3001 acgtaaaccg atctcatctc agtgttcagg tctttgtttc cagtctatat tgaataggga
     3061 tttaaactcc ttgacttcac ccttgctttt gtcttcagtt tctttggctt tgatttggtc
     3121 tttaaaggct atgattccgt atcccattgg tgtatgaatc cctggagaaa gtacatagta
     3181 ggcttcatct cccttaatcc aggttgcgct atcaaagtcc atcacccaaa agtgagcctt
     3241 atcgagactc aaatcattgg attgagcata agccatcatg cacccaatat cgtcaaactt
     3301 gtgaactttt ccttttgtaa caatctcagc cgagtagtga aaatcatcta tcatcatccg
     3361 acagtattcg cacatgtcgg ttcccttgtt gatagaaaca ggagtatttt ccgactgaca
     3421 gccaataatt aacaaaaatg aaaagattgc tattttaata ccaattcgat ttttcaataa
     3481 tgcataaaga agcctgtcat taggaagatt aactgtaggg gcgggtcttg cacccgccct
     3541 atctcctggg caaccgcaag ggttgcccct acaatcactc gcggtgacaa aacccctttt
     3601 gaacgaacta ctgattgcta gaattagaat tagcctccgt gtatttgata aagatttctt
     3661 caagtgatga gtcttctgtc cataagtcca taacgttgaa cccatattct acgagagttt
     3721 taatcacctt tgatttatac tctggaggga taaagaacct gagtctattt ccattaattg
     3781 tagcgttctt tgctcctaag tcctcgacaa gactgagtgc ctgtgaactc agattttcga
     3841 ttactacttc aagtattgat cccattttaa gcctggtcct taggcctcct aatgtatcta
     3901 gtgccacaac cttcccagaa acaagtatag caatcctgtc ggctagttct tctatataac
     3961 tgagtatatg agatgagagg agaatcgttt tccccttcct tttctcttcc ctgataagcc
     4021 ttttaaaagc tattcttccc tcagggtcta aactgattgt aggctcgtca aggataagga
     4081 ttggagggtc agaaataatc gcctgggcaa gagcaagcct ctgaagcatt ccacctgata
     4141 agactccgac ttttttattt ttctgctcaa ggattcctac cgcgctgagg acattttcca
     4201 aattattatt atttattcct ctaagagatg cataaaattc aataacttca agcacgctta
     4261 agttctcatg aaaagagatt ctctggggaa gatatccgat tctctttttt atctcttttg
     4321 gatttttact aacctcaata ttatcaactg tgatttttcc accacttgga tgtactaacc
     4381 caacaatagt gttgagaaga gtggttttac ctgcaccatt tggaccgact atggcaaagg
     4441 tctcagcctt ttcgatatcg agagaaactc cta
//
LOCUS       DATDRL010000268         2799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2099137, whole genome shotgun sequence.
ACCESSION   DATDRL010000268 DATDRL010000000
VERSION     DATDRL010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2099137"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VI935_08935"
     CDS             complement(<1..765)
                     /locus_tag="VI935_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLE25760.1"
                     /translation="MPKDIDMKVVRTTLFNPGWYIRENREKWVLPLILKDRAKRFRKR
                     PFLQYQNEKPLTFKQVNTFANKLANGLIKLGIGKGDRVAVLMPNSTEYILIWFGILKA
                     GGVMAPINTAYKMDFLHYIIDNSDSRFIFIGEEHLERLIPIQDRIPNVEKIVACTTNF
                     SNDFKNPGIALKPLISYRELIDNSSSSEPEVDVGFTDISRLMYTSGTTGRSKGVLKSH
                     AADYYSARGYVEVAEINENDVLYTCLPLFHSNAQVLC"
     gene            1015..1482
                     /gene="rimP"
                     /locus_tag="VI935_08940"
     CDS             1015..1482
                     /gene="rimP"
                     /locus_tag="VI935_08940"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HLE25761.1"
                     /translation="MSSLDQRVIDSVREILDPLLLQEGLELVDIEYRREGRGWVLRIY
                     IDKEGGVSVEDCARISRELGNLLDLNDIIPGTYNLEISSPGLTRALKKVRDFESFKGK
                     LVKIKTMTDIQGRRVFIGRLIDFVGNVASVEMDGHVYFIPYDEIERANLELDF"
     gene            1495..2781
                     /gene="nusA"
                     /locus_tag="VI935_08945"
     CDS             1495..2781
                     /gene="nusA"
                     /locus_tag="VI935_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HLE25762.1"
                     /translation="MASELSRVIDSVCKDKGVDKDAIVGAVVEAVLSAAQKLFRMQDK
                     GKELEVRYNEDEGEVELFEFKNVVTYVANPDIEIGVGEAAELDPEAEVGDLIGVKINP
                     DFTRIAIQNAKQKILQKLKEAEGKVIYEEFKSRKGELVSGIVRRIERKNVIVDLGKTE
                     AILPAEHQVSKEYYKPKERIRAILFDIRETKRGPQLILSRAHPDFVSKLFITEVPEIT
                     EGVVQIKAISRDPGSRTKISVSSNDPDVDPIGACVGMRGSRVQNIIHELRGEKIDIVP
                     WSSDHARFVCNALSPARVSKVIIDEDNRSMEVIVDDDQLSLAIGRKGQNVRLASQLTE
                     WRIDIKTDSQVRREQQESVSLLMTLPGVGEVTANLLYGEGFRSLEDIAFSDPKSLVKA
                     AGLKKEEDAIKIQTAARIALKDKLEKMSVSAKKEEG"
BASE COUNT          873 a          452 c          647 g          827 t
ORIGIN      
        1 acatagcact tgggcattag agtgaaaaag gggcaaacag gtataaagaa catcattttc
       61 gtttatttca gctacctcta cgtatcctct ggcgctgtaa taatcggcgg cgtgtgattt
      121 tagaacccct tttgaacgtc cagtggttcc tgatgtgtac attaggcgtg aaatatcagt
      181 aaatccaaca tctacttctg gttcgctaga gcttgaatta tcaattaatt ccctataaga
      241 aataaggggt tttagcgcga taccagggtt cttgaaatca ttgctaaagt ttgtagtgca
      301 tgcaactatt ttctctacat tagggattct atcttgaatt ggtattaatc tctcaaggtg
      361 ttcctcaccg atgaaaataa acctcgaatc cgaattatca atgatatagt gtaggaaatc
      421 catcttgtaa gcggtattta tcggagccat aacaccacca gcttttagta tcccaaacca
      481 tattaaaatg tattcggtgg aattcggcat taaaaccgct accctatctc cttttccaat
      541 tccgagttta atcaagccgt tagctaattt atttgcaaat gtattcactt gtttgaaggt
      601 gagcggtttt tcattttggt attgtaaaaa tggccttttc ctaaacctct ttgctctatc
      661 ctttaaaata agaggaagaa cccatttttc tctattctct ctaatatacc aaccagggtt
      721 gaaaagggtt gtccttacta ctttcatatc aatatctttt ggcatttaag taactccccg
      781 ttgaataggc gggatgacgt aaaataagta aaattataat tttgtatttc tacacctatc
      841 aatcaatcta tatagaatat tctaacttaa ggtattaaac tagtaagtgt ctagtagctt
      901 attacaatga aagtgttaga aacacctgaa tttgcctagt ctaatttagt taggtaaaat
      961 aaatttctga agcgggcatt tgacccgctt tttagttagt ttcggggtag atcgatgagt
     1021 tctttagatc aaagggttat cgatagtgtt cgggaaatct tagatcccct tcttttacaa
     1081 gagggattag agcttgtaga cattgagtat agaagagagg gtcggggatg ggttcttaga
     1141 atttatatag ataaagaagg tggagtgagc gttgaagact gtgctagaat cagtcgtgaa
     1201 ctgggtaatc tactcgacct aaatgatatt attcctggaa cttataacct tgagatatct
     1261 tccccaggac ttacaagggc tcttaaaaag gtaagggact ttgagagttt taaagggaag
     1321 ctggtaaaga ttaaaactat gacggatatt caaggtagaa gggtttttat tggtaggctt
     1381 atcgattttg ttggaaatgt agcttctgtt gaaatggatg ggcatgtata ttttattcct
     1441 tatgatgaaa ttgagagagc caacctagaa ttggattttt aggaggtgat gtagatggct
     1501 agtgaattaa gtcgagtaat agattctgtg tgcaaggata aaggtgtaga caaagatgct
     1561 attgttggag ctgtagtgga agcggttctt tctgctgcac agaagctttt tcgaatgcag
     1621 gataaaggca aggagcttga agtgcgttac aacgaggatg aaggtgaggt tgaactcttt
     1681 gagtttaaaa acgtagttac atatgtagcc aatccagaca ttgagatagg tgttggagaa
     1741 gcagcggaac tggaccccga ggctgaagta ggggatttaa taggggtaaa aatcaaccct
     1801 gattttacaa ggattgcgat tcagaatgca aagcagaaga ttcttcaaaa gctcaaagaa
     1861 gcagagggta aggtaatcta tgaggaattt aaaagtcgca agggtgaatt ggtcagcggt
     1921 atagtgagaa gaatcgagag aaaaaacgtg atagtagacc tagggaaaac ggaagctatt
     1981 cttcctgctg agcaccaggt ttccaaggag tattacaaac caaaggaaag aataagggca
     2041 attctgtttg atattaggga aactaagaga ggtcctcagc ttattctctc tcgtgcccat
     2101 cctgattttg tcagtaaact ttttattact gaggtcccgg agattactga aggtgtggtt
     2161 cagatcaagg ctatatcaag agaccctggc agcagaacga aaatatcggt ttcttcaaac
     2221 gatcccgatg ttgacccaat aggggcatgt gttggaatgc gtggttcaag agtccagaat
     2281 atcattcatg aacttcgagg tgaaaagatt gatattgttc catggtcgtc agaccatgct
     2341 agatttgtat gcaatgccct ctcgccagca cgagtgagca aggtaataat agatgaggat
     2401 aatagatcaa tggaggtaat tgtagatgat gaccaacttt cattagctat agggagaaaa
     2461 ggacagaacg ttagactagc atctcagctc acagaatgga gaatagatat aaagacggat
     2521 tctcaggtaa ggcgtgagca acaagagtct gtttcattac taatgactct tccaggcgtt
     2581 ggggaagtaa cagcaaatct gctttatggc gaggggtttc gttcacttga agatatcgcc
     2641 ttttcagacc caaagtcact tgtaaaggct gcaggtctta aaaaggaaga agatgccatc
     2701 aagattcaaa ccgctgcaag gattgctctc aaagataagc ttgaaaaaat gtctgtgtca
     2761 gctaagaaag aggaaggata aagattaagt tataggttc
//
LOCUS       DATDRL010000269        14283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3524454, whole genome shotgun sequence.
ACCESSION   DATDRL010000269 DATDRL010000000
VERSION     DATDRL010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14283
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3524454"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(449..889)
                     /locus_tag="VI935_08950"
     CDS             complement(449..889)
                     /locus_tag="VI935_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HLE25763.1"
                     /translation="MKILSKHVVVMVLSLFFVGMTLQSANSADKSLYDRLGGKAAISA
                     VVDQFVSNVAADTRVNMAFANTDIPHFKQCLVDQICQATGGPCKYTCRSMKDTHAGLG
                     CTADNFNAIVEDLVAALNKFKVGKKEQDELLGILGPLQSDIVEK"
     gene            complement(1046..1648)
                     /locus_tag="VI935_08955"
     CDS             complement(1046..1648)
                     /locus_tag="VI935_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25764.1"
                     /translation="MKKGTMVTLCLMVVFSVVILLANPGFPQIQDIEGNVICVEVDEK
                     GNVNGMDQFTECDGVFMVVGKDGKLYTVYGTEEQMKMMAKSPKKKVSGQVGGSQRAWV
                     IYGSPLDIQKGTQQSVTGTIVCLLPSYEKGNVTPVVSTGPCNEATPHAHVIYTKDGQV
                     YTLSGSEAAISSIEKNPQRTNVTLSGKVVGNQGAWILYVE"
     gene            complement(1740..1916)
                     /locus_tag="VI935_08960"
     CDS             complement(1740..1916)
                     /locus_tag="VI935_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25765.1"
                     /translation="MKTRQVSVVCLFPDNSNGKVKPVMALVTKDGEFYPLMDSEDLTH
                     KLDINEKKIYESRY"
     gene            2276..2518
                     /locus_tag="VI935_08965"
     CDS             2276..2518
                     /locus_tag="VI935_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25766.1"
                     /translation="MANIISNSCLNHLFKYAYRWLCLKRSKHPPSSEVWDLRRNWNEI
                     KEFVIEDFSKGCYRLSIQKKIRLSIGEIIALSCPAI"
     gene            3673..4086
                     /locus_tag="VI935_08970"
     CDS             3673..4086
                     /locus_tag="VI935_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25767.1"
                     /translation="MKTLKFLLVATLITLSLATVGFGQQGGRRGGGGHGGGGWHGGKG
                     AHGLHGGGGWHGRHGGWYGGKGGHGWHGGGGWHGYYYPRNQFYFNLGIPFLYSPYYYP
                     YGYYQPYYNPPYQYPPYYPYGAYPYMNFGFQLYGR"
     gene            5463..5918
                     /locus_tag="VI935_08975"
     CDS             5463..5918
                     /locus_tag="VI935_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25768.1"
                     /translation="MKGNNNSKRHSFILSRSRPYRKNDNPHVEQKNDDKVRKLVGYWR
                     YDTPEVVDLLNKLSEKADLLDNFFIPSSKLIKKIRDGNGRVIRRVYDRSRTPFQRLIE
                     CEELSEQEKQKLKKIFKSLDMIELRQENNKILQNLSDIKLQSSRKRIMI"
     gene            6708..8174
                     /locus_tag="VI935_08980"
     CDS             6708..8174
                     /locus_tag="VI935_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLE25769.1"
                     /translation="MRLTSNMETQKVKTIDLESLKQKLNSMSLFSQLSSDDLKGLVEK
                     SSLRQFEAGSVICREGEYGDTAFAILGGTAEVSINTAGYKNLTLALLGEGNIFGEMAA
                     LSGYPRSATVSAKEALYLLEIPAEVLKNLMKHSPGFKDAIEGMYAERAVKTYLRKVPL
                     FSALDDSVIEKLEKDVNLKSYKSGDIIFNEGDQGDSLFVIRTGFVKITKKHLDKEQII
                     AYLSQGNYFGEMALLEDERRTATVSAFTNVEVLQVLKEDFNMLLDADSKLAEGIRDII
                     IERKLRTLKVQKDPSKSERLQALVEKGIIQAESLLIIDLKTCIHCNNCVDACEDRHGY
                     PRLDRRGTRVADVSIPVACRLCHDPVCLICNFDAIKRAPTGEIYIIDDKCVGCAGCAI
                     RCPYNVIQMVPSKPEHELKKFNIISALLKGVKEDDNKTPKDHKIKPKHLAIKCDNCMG
                     YHDTACTNNCPTNAIRWVNPIKYFLDTEDLIPKREKYQ"
     gene            8171..10546
                     /locus_tag="VI935_08985"
     CDS             8171..10546
                     /locus_tag="VI935_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HLE25770.1"
                     /translation="MTSIPRTHKRLSLFTLVTGFIFWAVYRFAPNLPIPGVEKGTVYL
                     LGIVAVLLILFVMTYSLRKRVVKGVPGRLDNWLWAHIYLGILALFIIALHAEFRFGWD
                     YNTAAVIFLALVIITGVVGRYFFTRFPSSIASEQEKVISQINDVKKFIDDLMTGKSRP
                     FQKIIGSELNTPSPLSSTPEYWEELLEKGGILHEEEKEDFKKVVILLEQKASLEAQSV
                     SQLKYKPLFRGWLVAHLVVTGGLIVLIPLHILDDSFRAFQPTASDFGSPQECRQCHQR
                     QYDEWIMSMHAYGQVSPVAFELNAKVQEDSKGKVGVFCFKCHAPVSIAIGEDAIMPND
                     KRAPIGVLGVQCDSCHTIGKNHGLVSGDFPLEPGRTKYGPFGAGSNGDFKPVRNSFHK
                     SLDFNYLKTSEFCGSCHDVVTPKGLRVEETFAEWKDSIYAKKGITCQECHMRTIPGKP
                     GQKKVMGPAAIMVGVDLPERPLSDHSMIGVDYHLVDFFPYPDNPEETERIQREYMQEV
                     YELHKGSAQMKVEAPKSVITGSDFQVAVHVTNVGAGHYLPSGFTVERQLWVEIIVKGS
                     DGKLLFVSGDLDGNYDLRNRCSQEVKLEAAPLDEYLVNFQSEMIRVNPDGTEEDVFLT
                     SQANKFVKDGIPPLETRSGIYPITVPPDAKGPLKVDVRLRFRNLSPLIFDRLALDEKL
                     KKRLKIIDLATESRLIEVESNGMVTAGQQVSLSSSSGSDANAGTPSSQQGKIVGIIMS
                     IDNQAGTISLTDAQRQKHVIKIDPKLLEGISICNKVEVEVENGVAKSIKKL"
     gene            complement(10626..11054)
                     /locus_tag="VI935_08990"
     CDS             complement(10626..11054)
                     /locus_tag="VI935_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25771.1"
                     /translation="MDTQELKKRVLRDNVFSFLILFFLLASIAAGGDYIAQLKNLVVL
                     KVSDEQFQIKGEIVNNTDKELKRFSIKCIIYNPSGEVVEEAQILPFVIPIPPRGSSPF
                     LLPITYKPDMGRFTLQVINFVGKELSVDTGGKPLAFSVPR"
     gene            complement(11246..11638)
                     /locus_tag="VI935_08995"
     CDS             complement(11246..11638)
                     /locus_tag="VI935_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25772.1"
                     /translation="MKTSFALIFCLVLIFGIYIAESKSGGCPEGTVKVPNSNLCIEKL
                     NPARTANFKEIAMHCLSKKMDICLAEQIVKACEEGSIKVPPDQSSLLFLTSSGMYLRI
                     TPDCNLAKTGPIGSTEKEQFLCCDKLAE"
     gene            complement(12024..13712)
                     /locus_tag="VI935_09000"
     CDS             complement(12024..13712)
                     /locus_tag="VI935_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009672788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HLE25773.1"
                     /translation="MEERIWHKNYDIGVPTALKYPQIPVFTLLENSAQSYPQRDALIF
                     LGKRINYSQLLNLSNKFASSLVKLGIEKGDRIALFLPNTPHMIIAFYGALKAGAIVVN
                     TNPLYTERELEYQLKDSGADSIVTLDLKLTISKVKAVKDTIPLRHVIVGSTGDFLPFP
                     KNMLYPFLKRGELDAVPHSGSYHRFSEIIRNASDPLPTIVKPDDTAVLQYTGGTTGIS
                     KGAELTHKNLVTNAFQIRCWGEDVFIDGEEAILTVLPCFHVYGMTVCMNLGMMIGATL
                     LLLPRFNPKEVLQTIKEYRPTIFPGVPTIYTAIANHPQVNNYGVESIKLCLSGGAPLP
                     LEVLDKFENVTGTKILEAYGLSEASPATHANPFIGRREIGTVGLPIPDTDAKVVDLES
                     GEKELPVGEPGELIVKGPQVMKGYWNRSDETSLTLRNGWLFTGDIATMEENGYFSIVD
                     RKKEMIISGGYNVYPREVEEVLYEHPKVLEAAVIGVPDSYKGEYVKAFVIVKSGEEVT
                     ESEIILFCKERLAPFKVPKVVEFRTSLSKSTIGKVLRRALREEELKKTLNPHLK"
     gene            complement(14073..>14283)
                     /locus_tag="VI935_09005"
     CDS             complement(14073..>14283)
                     /locus_tag="VI935_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25774.1"
                     /translation="LINLSLKSRFTNSAEIKTEIKEAINAIVTENSNPRDPISGKKVS
                     NTDIKTIKASETSTFLERNISLKVI"
BASE COUNT         4104 a         2937 c         3075 g         4167 t
ORIGIN      
        1 cttaaaagtg gattcccgct aaatacttgc gggaatgaca acttctgttg gggattttaa
       61 cacctttcca atggaaatgg tgttactact aatgagagta gtctgagaat tagcctattt
      121 tcaacaacaa accctttgta aaaaagggat aatttaattt cgctcttcag agtcagtaat
      181 gtagggctct ttcctccgaa cgagcctttg tattcaggac tgatgaggga aacattataa
      241 aggcaatcat taccaggtta agcgatatca cgcttccctg gcagacagcg tgtgaacggt
      301 agcacctgtg gcgggcaagt atcatgcccg ccaacagggc aattcccatc taaatttgaa
      361 tagttaagtt aaggtctgct tctagtgttg tgcctgttct cagaaaaccg ttcatcaatt
      421 tcttaatgca acggctcgga actggttgct atttttcaac tatgtcagat tgtagcgggc
      481 caaggatacc tagaagctca tcttgttcct tctttcccac tttgaatttg tttaaggcag
      541 caactaggtc ttctacgatt gcgttgaaat tatccgccgt gcatccaagt ccagcgtggg
      601 tatccttcat gctcctgcag gtatacttgc agggaccgcc tgttgcctga cagatttggt
      661 caacaagaca ctgcttgaaa tgtggaatat cagtgttagc aaaagccata ttaacgcgtg
      721 tgtcggccgc cacattggat acaaattggt caacaacggc tgaaatagcc gctttgcctc
      781 caagacggtc gtatagcgat ttatctgccg aattagccga ctgaagagtc atgccaacaa
      841 aaaagagcga taataccata acgacaacat gctttgataa gattttcatg ttataccctc
      901 ctttaaggat tttttttgtt ctattaagta aaatagatcc tatagcaact gttaacaaaa
      961 tgtcaattat taatcttcta tgaatctttt agggggacag aacaaataat acctctcccc
     1021 tcaccttagt ctcgtgatct caagtctatt ctacataaag aatccaggca ccctgattcc
     1081 caactacctt tccacttagc gttacatttg ttctctgagg attcttttct atactactaa
     1141 ttgctgcctc cgaaccgctt aatgtgtata cttgtccatc cttggtgtat ataacatgtg
     1201 catgtggtgt cgcctcatta catggcccag ttgatactac tggtgtaacg ttacccttct
     1261 cgtaactagg cagcaagcaa actattgtac ccgttacact ctgttgtgta cctttttgga
     1321 tgtctaatgg tgatccgtat ataacccaag ccctctgact accaccaacc tgtccgctga
     1381 cctttttctt agggcttttt gccatcatct tcatttgttc ttctgttcca tagacggtgt
     1441 atagtttgcc gtccttccct acaaccatga atacaccatc acattcagta aattgatcca
     1501 taccattaac gttacctttc tcatctacct ccacgcaaat aacattgccc tctatgtcct
     1561 gtatttgtgg aaagccaggg ttggccagta aaattacaac agaaaaaact accataagac
     1621 ataaagtaac catagttcct ttcttcatct gatttacctc cagtttgttt tgattttgtg
     1681 aaattatacc ttaaatctaa gtcgtttgat ttttagatta accaaattca ctacttctat
     1741 taatatctac tttcatatat cttcttttcg ttaatatcta gcttatgtgt tagatcttct
     1801 gaatccatta atggatagaa ttcaccatcc ttagttacta aagccattac tggtttaacc
     1861 ttcccgttac tattgtcagg aaacaaacat acaacactaa cttgccttgt cttcattagt
     1921 gtctcacatg aaatgctagg acttggtaac catattgaaa acactatact gctccccatt
     1981 aagaacctaa taaaaataag ctagtaaaat atatagaaaa aggtagaagg agtgggagca
     2041 atgaaacgga gaagatggga ttctaaaaca aagaccaaga tagtgcttga aggtctatcg
     2101 ggaagacccg cctctgagtg caagattcat acttcacatt taaacgccgc ttaaccgaag
     2161 ggcatctgta acaccttcgc attgggtaca caccgtctct atacccagca aatccacatg
     2221 gctaagaagc cattctctag acatactcac agctaaggaa acagagctaa ccctgatggc
     2281 taatataatc tcaaattcct gccttaacca tctctttaaa tatgcttatc gttggctttg
     2341 cctcaaaagg tctaagcacc ctccttcatc agaggtttgg gatttacgca gaaactggaa
     2401 cgaaattaaa gagtttgtaa ttgaagactt ttcaaaaggc tgctacaggc tttctatcca
     2461 gaaaaagata aggctctcta taggtgaaat tatagccctt tcttgtcccg caatctaatc
     2521 gaatagattt ctctaaatcc ggggttggaa aaccccggct atcgcttgca ctaacaaagg
     2581 atagacggga cattcctgtc ccgttctatc gggtgagggc tatcactgta gagaatttca
     2641 tggaacgtgc tacccggctt tacgagcatg agccggaagg aggagtctcc tcccacaggc
     2701 ttgagttgta cgttaatcgg tggtttaggt gggtccacta gggacaagtt gcggatggtc
     2761 ataggggctg cttgccccgt gcgccgtgaa tgccggctgt ttcgctgcat gtgttgggcg
     2821 ggcggggcaa aattgatatt gtggtgtgat tggaaggcat gaatgatgga tcgagctagg
     2881 tacaagacgg gccgtagcgc ctgggggtat tccctccgag ttagaagctg catgcctgtc
     2941 tgcacccaag aagggtcacg caattgtact cttctacgca atcctaagac caggacgatt
     3001 cacaacatga ggggaaagtc atttggcaca cacattctac caaggggtcg caactggggt
     3061 tcagcgggcc gcctgtctcc tctgtggaga gtgggtacat tcagcgcagt cgcagggggc
     3121 ctctgggttt cctggaggga aggtttgtac caccacgtct ttttcccatg ccttgccaat
     3181 agggtcgact tcgcatacac agccgttacc accacaagag gcaccaatgt tacaaagtgc
     3241 aaggcggaga ccccctccaa gtatttaacg caagcacaaa tagcctgttc ttcacaaaac
     3301 tcaagacaac caccgtcgcc gttcaatccg ctcttggcgg ggggtgtcca tgcaattaag
     3361 accacggcac tcattacaac tgctaccgtt aaagaaataa agaatcttct catcccctca
     3421 tgcctcctct aatttagatt gaacatatat aaactacatt tcttatcata agtcaaaaac
     3481 tttgaataaa aatacagcct tagtcctata tcacctcgtg ttgatccagg ttgccctatt
     3541 ccgaacgcgc aactttagtg aaacctttgt cgggacctcc agttaataga gttatagacc
     3601 ctaggagtgg tttgatacaa tatgctatac tttataccta acatgaaaag gggttatggt
     3661 tggaggtgtt aaatgaaaac acttaaattt cttttagtgg caacgctaat tacgctatca
     3721 cttgcaaccg tagggtttgg tcagcaaggt ggtcggcgtg gagggggtgg ccatggcggt
     3781 ggaggttggc atggcggaaa gggcgctcat ggattgcatg gcggaggtgg ttggcatggc
     3841 agacatggtg gatggtacgg tggtaagggc ggtcatggat ggcatggggg tggaggttgg
     3901 catgggtatt actacccacg gaatcagttt tacttcaact taggaattcc gttcctctat
     3961 tctccctatt actaccctta tggatactac caaccttatt ataatcctcc ttatcaatat
     4021 cctccgtact atccgtatgg agcgtacccc tacatgaact tcggattcca gctctatggt
     4081 cgttgagtca agtgtgttta gaggtagaat agcacaggag ctaaagggca cggcaaagta
     4141 atagtgtagt ctgagatacc ccgtattggc acagtcctcg tcaagaaatc gctttttagg
     4201 acgagtgtga aactctactt gttgattttg atgcctaaac agaatccgat ttgctgagtt
     4261 gcacaaaaaa actagggagc ggagaaaggg tttgacttcc ttgagtatca cataagccca
     4321 gagggtcttt ctctagcgaa aaagactgtt gagaatttca tatcacgtgc cacccggctt
     4381 tatgagcatg agccggaagg aggagtctcc tcccaccgcc ttgggttgta cgttaatcgg
     4441 tggtgcaggt gggggaacct tcggccttcg tggtgcctat tgagcaacat gtgcctatgg
     4501 gggggtgcaa tcaagggggt ctccaacgac ggtgccacct tgcgctacca cgttgacgta
     4561 ttgtaccaag cattgccgac atgcttctaa ctgctctgca ctgagatcta ctacgttgta
     4621 tcctggtgcg ccaagcacag gacagccagg gggctcggta ttggtcccta ggatggtatt
     4681 cgcgacgcat tgggcgtttc cgtagaatac cccagcttgg aattcgtcca gcgggaattg
     4741 gttaaagatg tctgtcagtt ggcacgtgcc tgggacgttg ttgtcctccc atattccttc
     4801 cggccagcat tctgcagtcg gcggtatggc gaggaagtag ttgcatggac accctgggca
     4861 ttgatcgacc tcacacggtg gctcggggtt ttcgacgggt gaggactgaa ccacaacgtc
     4921 cttttcccac agcgcacata taggatccac atcgcattca caatggctcc cgggattttc
     4981 agtgtcaaca cactgggcac caattttaca ctttgttagg cgcaggcttc ccccgagtat
     5041 tttagcgcac atacaaacgg cctgttcctt acaaaactca agacaaccac cgtcgccgtt
     5101 aaatccgctc ttggcggggg gtgtccatgc aattaagaac atggcgctca ttacaactgc
     5161 taccgttaaa gaaataaaga atcttctcat cccctcatgc ctcctctaat ttagattgaa
     5221 catatataaa ctacatttct tatcataagt taagaacttt gaataaaaat acaaccttag
     5281 tcctaccatc taactcgata acctagatct tctcgggctg ggtagttaga gcagctggat
     5341 taggaaagaa cgaaaatagt gtattacgta ttgatagaac agcacagagt agaatattcc
     5401 acagggtatt tcactatcac ccagacaacg ataaatcgat actcaaggta ttattcgaga
     5461 ggatgaaggg aaataataat tcaaagaggc actcattcat cttatctcgt tcaagaccat
     5521 ataggaagaa tgacaatcca cacgttgagc agaaaaatga cgataaggta aggaagctgg
     5581 ttggttactg gagatatgat acgcctgagg tagttgatct tctcaataaa ctctctgaga
     5641 aggcagactt gctggataat ttctttattc cctcttctaa attgataaaa aagataagag
     5701 atggtaatgg aagggtaata cggagggtgt atgataggtc caggactcct tttcagagac
     5761 ttattgagtg tgaagaatta tcagagcagg agaaacagaa actaaagaag atattcaagt
     5821 cccttgatat gatagaattg agacaggaga ataacaaaat attacaaaat ctaagtgata
     5881 ttaaactaca gagttcgagg aaaagaatta tgatctaaca aggatttaaa atatccaatt
     5941 tcgaggaact ataattgtcg tatagatgtt gcgggaaatt gttaattgat agaaatcact
     6001 tagaagcagg tacttagaat aatatccgct ggttattttt ggacaagcct ataaggggaa
     6061 cgtgttaata agaatcgcta ggaatcattt attctttatg attccaacct ataccaagct
     6121 tgacagaagg gtgcagtaca acactaataa taacattact aacaaaccaa ctaatcgtac
     6181 tctacttatc atcttcttcg cctcatttaa ttcttactga atatatacga ctattgggca
     6241 caatttggat taacccaggt tcaagtttta ttaatgaatt acgacataaa gtaatagtca
     6301 actggagatt ggccaaaaga taagaatata tatttgagga tacaacttta atttttgcta
     6361 ctatctttaa ttgtcatcaa aaaccaaata ttgtagagcc cttataatca tcaactttat
     6421 ttacacttgt gtatacagcg tttacgttct gatatcaatt ttatgatcaa gcatctagct
     6481 agcatacttt aagccacttt ttttacaccg cacttattat ctttattggt catggactag
     6541 atgttggcac gctcattgca catcagacaa atatcagaac ggtgacgagc gggaaatgaa
     6601 aaagttatct gcagttgagg ttttaaatgt tagtcttttt ctaaattcac tatatttgtt
     6661 ataatagaaa taagttaatt taggtttggg tgtttcgggg cgggtaaatg agactaacgt
     6721 caaacatgga aacccagaag gtcaagacaa ttgacttgga atctcttaaa cagaaattaa
     6781 acagtatgag tcttttttct cagctctcgt ctgacgatct aaaagggcta gttgaaaaat
     6841 caagcctgag gcaatttgag gcaggttctg tgatatgccg ggaaggagag tacggtgata
     6901 cagcctttgc tatcttaggt ggtaccgctg aggtaagcat taacaccgca ggatataaaa
     6961 atctaacgct tgcccttcta ggagaaggga acatattcgg tgaaatggct gcgctttctg
     7021 gttacccaag aagcgctacc gtgtctgcaa aggaggccct ttaccttcta gagattcctg
     7081 ctgaggtgct caagaattta atgaagcatt ccccagggtt taaggatgct atagagggta
     7141 tgtatgctga gcgagcggtt aagacatatc ttaggaaagt tcctctattt tcagccttag
     7201 atgattctgt aatagaaaag ctggaaaagg atgtaaatct aaaatcatac aaatctggcg
     7261 acattatatt taatgaggga gatcaagggg attctttgtt tgtgattcgg acgggctttg
     7321 ttaagattac gaaaaaacac ctagataaag agcagatcat tgcttatctt tcccagggta
     7381 attactttgg tgagatggct cttcttgagg atgagaggcg taccgctact gtatctgcat
     7441 ttactaatgt agaggttctt caggtattaa aagaggattt caatatgcta ctggatgccg
     7501 attcaaagct agctgagggg ataagggaca taattataga acgcaagctt aggaccctta
     7561 aggttcagaa agacccttct aaatctgaaa ggttacaagc tcttgttgaa aaggggataa
     7621 ttcaagcgga gagtcttctg attattgacc ttaagacctg tattcactgc aacaactgtg
     7681 ttgatgcttg tgaggacagg cacggttatc cacgcttaga tagacggggg acacgggtcg
     7741 ccgatgtatc tattccggtt gcctgccgat tgtgtcacga cccagtgtgc ctaatatgca
     7801 actttgacgc gataaagcgt gcccctactg gtgagattta tattatagat gacaagtgcg
     7861 ttgggtgtgc gggatgtgca ataaggtgcc cttataatgt tattcaaatg gtaccttcaa
     7921 agcctgagca tgaactcaaa aaatttaata tcataagcgc cctattaaaa ggtgtaaaag
     7981 aggatgataa caagactcct aaagaccata agataaaacc gaagcatctg gctattaaat
     8041 gtgacaactg tatgggctac cacgacacgg cctgtacaaa caactgtcct acaaacgcaa
     8101 taaggtgggt taatcctatt aagtattttt tggatactga agacttaata ccaaaaaggg
     8161 agaaatatca atgaccagta ttccacgaac gcataagaga ttatccctat ttaccttggt
     8221 tacggggttt atcttctggg ctgtttatcg ctttgctcca aacctaccta ttcctggtgt
     8281 ggaaaaagga acagtttacc tccttggtat tgtagcagtc cttcttattc tctttgtgat
     8341 gacatactct ctacgtaaac gtgtggttaa aggggtacct ggtcgccttg ataattggct
     8401 ctgggctcac atttatctag gaatcttagc actttttata atagccctcc atgctgagtt
     8461 ccgttttggt tgggattaca atacggcagc ggtgatattt cttgccttag tcattataac
     8521 cggcgttgta ggtcgctatt ttttcacacg ttttccaagc tcaattgctt ctgaacaaga
     8581 aaaagtaatc tctcaaatta atgacgttaa aaaatttata gacgatttaa tgaccggaaa
     8641 gtctagacca tttcaaaaaa ttataggctc tgagcttaat accccttcgc ctctttcttc
     8701 tacacctgaa tactgggagg agcttcttga aaagggtgga atcctacatg aggaagagaa
     8761 ggaggatttt aaaaaggtag ttattttact tgagcaaaag gcaagtttag aagcccaatc
     8821 tgtgagccag cttaagtata aaccgctctt tcgtggttgg cttgttgcgc atctcgttgt
     8881 gacaggtggg cttattgttt tgataccact tcatatcctg gatgatagct ttagagcatt
     8941 tcaaccaaca gcttcggatt ttggatcacc tcaagaatgc aggcagtgtc atcaaaggca
     9001 atacgatgag tggataatgt caatgcacgc atacggtcag gtaagccctg ttgcctttga
     9061 gctaaacgca aaggttcagg aggacagcaa gggaaaggtc ggggtctttt gttttaagtg
     9121 ccatgctcca gtaagcattg cgatcgggga agatgcaatt atgcctaatg ataaaagggc
     9181 acctatcggg gttctaggag tccagtgtga ctcttgccat actataggaa agaaccacgg
     9241 gcttgtaagc ggggattttc ctcttgagcc aggtaggaca aagtatggcc cctttggcgc
     9301 aggatcaaat ggcgatttta aacctgtgcg caactcattt cataagagct tggattttaa
     9361 ctatcttaaa acctctgagt tttgtggctc ttgccatgac gttgttactc ccaagggact
     9421 tagggttgaa gaaacgtttg cagaatggaa ggacagcatt tacgccaaga agggcataac
     9481 atgccaggaa tgccacatga ggacaatccc tggtaaacct ggtcagaaga aggttatggg
     9541 tcccgcagcc atcatggtcg gtgttgattt gccagagagg ccgctctctg atcactctat
     9601 gatcggcgtt gattaccatc ttgtagactt tttcccatat ccggataatc ccgaagaaac
     9661 tgaaaggatt cagagggagt atatgcagga ggtttatgaa cttcataaag gaagcgcaca
     9721 gatgaaggtc gaagcgccaa aatcggtaat cacaggatcg gattttcaag ttgcggtgca
     9781 tgtaacaaac gttggtgcgg ggcactatct tccatccggt tttacggttg agcgtcagtt
     9841 atgggttgag ataatagtaa agggctcaga tgggaaattg ctttttgtat ctggtgactt
     9901 ggatggaaac tatgatttac gtaaccgttg cagccaggag gtaaaactgg aagcagcacc
     9961 tttggatgaa tatctagtca actttcaatc cgagatgatt agagttaatc cagacgggac
    10021 tgaagaagac gtctttttaa ctagtcaggc aaacaaattt gttaaagatg gaatacctcc
    10081 ccttgagaca agatccggaa tctaccctat tacagttccg cccgatgcga agggaccact
    10141 caaggttgat gtgcgactta gatttagaaa tctctcgcca ttgatatttg accgattagc
    10201 tctagatgaa aaactaaaaa aacgacttaa gatcatagat ttggcaacag aatcgaggtt
    10261 gatagaagta gaatcaaatg gcatggtgac tgctggtcag caggttagtt tatcttcaag
    10321 ttcaggttcg gatgcaaatg ctggtactcc ctcctctcaa caggggaaaa tagttggaat
    10381 tataatgtca attgataatc aagcaggaac catatcatta actgatgcgc aaagacaaaa
    10441 gcatgtaata aagatagacc ctaaactact tgaagggatt tctatttgca ataaggttga
    10501 agtcgaggtt gaaaacggag ttgctaagtc aattaagaag ctatgataca agatgcatga
    10561 tccatgacgc acgatacaag ataagtgatt catcatgcat cttgtatcct gcatcctgat
    10621 ctaacttatc taggaacact aaatgctaga ggctttccac cagtgtcaac acttagttcc
    10681 ttcccaacaa agttaattac ctggagtgtg aaccttccca tatcaggttt atatgtaatc
    10741 ggcagtaaaa aaggggaact gccgcggggt gggattggaa taacaaaggg cagaatctgg
    10801 gcttcctcga ctacctcacc ggaggggtta taaattatac attttataga gaagcgcttt
    10861 agttccttat ccgtattgtt tactatctcg cctttgatct ggaactgctc atcggaaacc
    10921 tttaacacaa ccaggttctt tagttgggcg atataatccc ctcctgctgc tatagaagcc
    10981 aagagaaaga ataggatcag gaaggaaaaa acattatctc ttaaaactcg tttttttaat
    11041 tcctgcgtat ccataaactt aactgtttgt ataacgaagg caataagaca cgaagtttga
    11101 atttagaatt cagaagctaa gagccagaat aaaataaaca tggaaaatct ctgttcttct
    11161 ggcttctgcc ccctaacttc taagttaaaa ctcatattcc ttctttgtgt cttggtgcct
    11221 tcgtggctga ataattaccc ataaactatt cagcaagttt atcacagcac aaaaactgtt
    11281 ctttttcggt agagccaatg ggacctgttt ttgcaaggtt acaatcggga gttatacgta
    11341 aatacatacc acttgatgta agaaataata gcgagctttg atcaggtggt acttttattg
    11401 aaccttcttc acacgccttt acgatttgtt cggctaggca aatatccatt ttcttggaaa
    11461 ggcaatgcat ggctatttcc ttaaaatttg cggttcttgc tgggtttaat ttctcaatac
    11521 aaaggttgga attggggact ttcaccgtgc cctcgggaca gcctccgctt ttggattccg
    11581 ctatgtaaat accaaatatt agtactaaac aaaaaataag tgcaaagctt gttttcataa
    11641 cgattccgtt attaaagatc ataaacacaa tataactcag accactttaa tgtcaaaaat
    11701 gtcggggatt atgacctcat cagtctctta atttttttga ttagaaatta attccttccc
    11761 tattgaagat ggaaggcctc gtcgtgagtt cagacgagcg gatagcatga gggaatgata
    11821 tggctgatgc tcgattacaa catttaggaa tgaaagaaag gtgtcattcc cgcacatatt
    11881 tagcgggaat ccataaaaga aaacatggat ccccgataac tgcactcggg tatgacagca
    11941 agagcacctt ctagactcga gaatgacaaa aattctccca ccaccgcaga accggcatcc
    12001 ccaatgcgat aatggttaaa atttcattta agatggggat taagtgtttt cttaagttcc
    12061 tcttctctca gcgctcttct tagaaccttt ccaattgtac tttttgagag agaggttcta
    12121 aactcgacaa ccttgggaac tttaaatggg gcgagtcttt ctttgcagaa aagaataatc
    12181 tcactctctg ttacttcctc tcctgatttt actatgacaa aagcctttac atattcaccc
    12241 ttatatgaat ctggaacacc tattacagcg gcttcaagca cctttggatg ctcatagaga
    12301 acctcctcta cctctcttgg ataaacattg tagcctcctg agattatcat ctcctttttt
    12361 ctgtcaacaa tcgaaaaata gccattctct tccattgtag caatgtcgcc tgtgaatagc
    12421 caaccatttc ttaatgtaag cgatgtttca tcggatcgat tccagtaccc tttcataacc
    12481 tgtggtccct tgactatgag ctcacctggt tctccaacgg gaagctcctt ttccccgctt
    12541 tctaagtcaa cgacctttgc atctgtatcc ggaatgggga gaccgactgt tccaatttcg
    12601 cgtctaccta tgaagggatt tgcatgggtt gcaggcgatg cttcagacag cccgtaagcc
    12661 tctaggattt tcgttcctgt aacattctca aacttatcta gtacctcaag tggtaaaggc
    12721 gctcctccag atagacatag ctttatggac tcgacaccat agttgtttac ctgagggtga
    12781 tttgctatgg ctgtatatat tgtggggaca ccgggaaata tagttgggcg gtattctttt
    12841 atcgtttgta gaacctcttt gggattaaat ctaggaagaa gcagtagtgt cgccccaatc
    12901 atcataccaa gattcataca tacggtcatc ccatagacat ggaaacatgg aagaacggta
    12961 agtattgctt cttccccatc tataaacaca tcttctcccc agcatcgaat ttgaaatgcg
    13021 ttagtaacca aattcttgtg agtaagttca gcacctttag atatccctgt tgttccacct
    13081 gtatactgaa gcacggcagt gtcatccggt ttaacaattg ttggcagagg gtcggaagca
    13141 tttcttatta tttctgaaaa tctatgataa ctccccgaat gtggtacagc atctaattcc
    13201 cctcgcttaa gaaatgggta aagcatgttt ttaggaaaag ggagaaaatc tcctgtactc
    13261 cctacgatta catgccttaa gggaattgta tccttgacgg cctttacctt tgatatggtg
    13321 agttttaaat ctaaggttac aatggaatct gcacccgagt cctttaactg gtattcaagc
    13381 tctctttctg tataaagtgg gtttgtatta actacaatgg ctcctgcttt taaagcacca
    13441 taaaaggcta taatcatgtg aggggtattt ggaaggaata gcgcaattcg atctcctttt
    13501 tctatcccta atttgactag agaggatgca aatttatttg aaagattaag aagctggcta
    13561 taattgattc ttttgcctaa aaatattagt gcatctcttt gtggatagct ctgtgcagag
    13621 ttctcaagta gtgtaaagac tgggatctgt gggtacttga gagccgttgg tactcctatg
    13681 tcgtaatttt tatgccagat tctttcttcc attttgaaat gcggtttacc ctttacctat
    13741 tctattttac tactatagct tactgtaagg caattgataa tttattttgt catttgagga
    13801 acgaagtgat gaagcaatcc caaaaagaga gattgcttcg cttcgctcgc aaagacaaca
    13861 cgggaaagtg tcgttgcgag ggagtaatac ggccgaagca atctcgtata caattaaggg
    13921 atttctacag tgggtttaca cttaataatg tattcgtaat cataggccgg agtagggatg
    13981 gcccgacgga tcacctctac aatgctaggt ttctaggtta gagaaaacag tgttaaagtt
    14041 aacactaaat ctgaaatggc atggttacta attcagatga cctttaaaga gatatttctc
    14101 tctaaaaaag tactcgtctc acttgcttta attgttttaa tatccgtatt gctaaccttc
    14161 tttccactta tcggatctct gggatttgag ttctctgtta cgatagcatt tatagcttcc
    14221 tttatctcag tctttatctc tgctgaattt gtaaaccttg actttaggga gaggtttata
    14281 aga
//
LOCUS       DATDRL010000270         5466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3717382, whole genome shotgun sequence.
ACCESSION   DATDRL010000270 DATDRL010000000
VERSION     DATDRL010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3717382"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..1286
                     /locus_tag="VI935_09010"
     CDS             303..1286
                     /locus_tag="VI935_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE25775.1"
                     /translation="MNKRLSYFIRPSIMSLMCITLIIIILFSPLSVLEISKALAQDTK
                     PPSGSKEFNKYYELGLKHYEEKKYEEAIKAFNKVLEINPKSADAYFKIGRCYSRLNKL
                     VESTKSYEKAIQIDPDGSYPIYFHLGLNYYNLKDYDKAIESLQKAIKIKPDTYYFTYF
                     SLANSYYNKKDYENAVKYLNETIKRKPDYEIAYFILSKSHQQLGNYKGAIKVLDDGLK
                     QKPNSLTIIESLAWLLSTSPPDELRDAKRAVILAERAVDFTKGEDPIHLDTLAAAYAE
                     AGRYQDAIKTQEKAINLLMNSQASSGIDSSLIEEFKKRLSYYKENKPWRIE"
     gene            1817..2446
                     /locus_tag="VI935_09015"
     CDS             1817..2446
                     /locus_tag="VI935_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25776.1"
                     /translation="MVRLSILILSIIFTLGLSSMLAYSHTDDATIVETFSCAVEEAED
                     PNEAEAHWWWCYDESAYDALHGLKNGAFSLSMKLDVFDEEDNLVITCEGETSTKSVDT
                     LSGNFAARLEEACVADECGTGVRECHVENVDLEEVFCGPEPQDVACLEQQEACDDAVE
                     DFESTGSFTYTGDVKATHIGTGGPSGNASSSGGGGGLEHHKAMAECPEF"
     gene            2960..4546
                     /locus_tag="VI935_09020"
     CDS             2960..4546
                     /locus_tag="VI935_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25777.1"
                     /translation="MNSGERTVDKAGDVSSAFTVFAFLFAWAQLFDYSAAVATIFDFS
                     TNYYDIDYCFTVAICLTAVWVILNPSSLYRSIFLFVFVIVGTIIRMPISPNHVVFEVI
                     VLISILTCFVYMKLKEKSSFTKTKFYEAFAPVVRLEVIVLYFWAAIHKINTGFFDKDI
                     SCATIQLFNVKDVLPIMPTPDWFISINPFLTLLTESSIPILLIIPKTRILGLIIAIIF
                     HFILGFVYTGFTILVYSFLSLFIPASSYDRIKPNFKQLTYKLSKILSKFSNYKDWKKT
                     RFDNFITQLIFLISIFFLMGFFMRGNPEKFYLLSEEGLYIIFFFVFLIAFFYFVIRGL
                     KEFRIEQRITLVPEMKWLLIFPILVLVNGSLPHLGVKNIQSMAMFSNLRTEGGKTNHL
                     FIPSFFQSSNNLEDLVTIKGANLRELNKFSGFSSRRPLRGTSVTLPRSYVKHMENNDK
                     DYRERFEYKVPFVLLQNLVTGLAKEGQKDIKLEYERDGEILYTRNAEVDPVLSKASIF
                     QRKFISQRAVPDDDRGLCMW"
     gene            complement(4774..5064)
                     /locus_tag="VI935_09025"
     CDS             complement(4774..5064)
                     /locus_tag="VI935_09025"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HLE25778.1"
                     /translation="MKKHKSKIPKFKNEEEEFDFWSSYDSTEFFDETEEISEPIEVTK
                     PKVRKQRITMLLDQRLKAQLQKIAEEKGVRYQTLIQMWLKEKVKQEIKSRIS"
     gene            complement(5225..>5466)
                     /locus_tag="VI935_09030"
     CDS             complement(5225..>5466)
                     /locus_tag="VI935_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HLE25779.1"
                     /translation="QVKSYKPSHGHWKRMMDVFKIPKENVLHVAASYVHDIVPAKELG
                     FQAVWINRKDKKVKGNITPDYEFRDLRPLVNLLEK"
BASE COUNT         1655 a          955 c         1016 g         1840 t
ORIGIN      
        1 ttaacctagt ttgccacaga gatcacggag cttagtaaag agtttgtttt atccattttt
       61 aggttcagtg tcattcttat catttgtgcc tggagtcaaa ttccttttca attttggatt
      121 gataatattt tatatttcaa gtcctgccct agtttttgtg ggttgagtct taggagaagg
      181 ttttggatgt agtgtctgcc caaggagtca cgccatttta ttgtaggtac ataatattgt
      241 ctgccccctt tttttcttta aaagtttgtt tataataaat caaaattcta aggagacatt
      301 aaatgaataa gaggctatca tattttattc gtccctcgat aatgagtctt atgtgtatta
      361 cgttaataat tattatttta ttttcccccc tatcggtact tgaaatatca aaagccctgg
      421 cgcaagacac aaaacccccg tcaggctcta aagaattcaa taaatactac gaacttggat
      481 taaaacacta tgaggaaaag aaatatgaag aagctattaa agcattcaat aaagtccttg
      541 aaattaaccc aaagagtgca gatgcatact ttaagattgg taggtgttac agcagattga
      601 ataagcttgt cgaatccaca aaatcatatg aaaaggcgat ccaaatcgat cccgacggat
      661 catatccaat ctattttcat cttggactca attactataa cctaaaggac tatgacaagg
      721 ctatagaatc tttacaaaag gcaatcaaga taaaacctga tacctattat ttcacatact
      781 tttcccttgc aaatagttac tataataaga aagattatga aaatgcggtc aaatatctta
      841 atgagactat caaaaggaaa ccagattatg aaatagctta tttcattctt tccaaaagcc
      901 atcaacaact tggcaattac aaaggtgcga taaaggtgct tgatgatggt ttaaaacaaa
      961 aacccaattc cctaactatc atagaatcat tagcctggct tttatcaacc tctcctcctg
     1021 atgaactgag agacgcaaaa cgagccgtta tattagctga aagggctgtt gactttacca
     1081 agggtgaaga tccaattcat ttagatacgc ttgcagcagc gtatgccgaa gcagggaggt
     1141 atcaggatgc cataaaaact caggaaaagg ctattaattt gctaatgaat tcccaagctt
     1201 cttccggtat tgatagttca ctaatcgaag aatttaaaaa acgcctttca tattataaag
     1261 aaaataaacc ctggaggatt gaatagattt aaacgcatat acctgtattg gattcacgag
     1321 ctagatgtca atacaaaacg aaaatgtcat atgtatgtag cggaaggcat tagccttcca
     1381 catttttctt tatggcgacc taaaggtcgc cgctacattc gcttactgaa agggggaatt
     1441 cccctcttct tgaaatccct ctctagctcc tctttggaaa aggggagtac aacacaagac
     1501 aattcctcct ttcgtaaggt gagatttagt gggatttccc acctgtcaat gaaaagtaga
     1561 tacgcataga taaaaaacta caccactcaa caaattttga caaaaaacta gtggcattat
     1621 cattttgtga caacataacc cataataacc attgatattt ttattgataa aaaaaactct
     1681 tgacatattt atttagtagt gctaaggtct tttgcaagtg gagcatttaa aaaatggggg
     1741 gcaaagagac taaaaattgt tttcaatttt ttagtgttca tcctaatcaa aaaattatta
     1801 aaaggaggaa aaggttatgg tcagattaag cattttgatt ttatctataa tattcacttt
     1861 gggtctatct agtatgttag cttattccca tactgatgac gcgaccattg tagaaacctt
     1921 tagctgtgca gttgaagaag ctgaggatcc aaacgaagca gaggcacatt ggtggtggtg
     1981 ttatgatgaa tcggcttatg atgctttgca cggtcttaaa aatggggcat tttcactttc
     2041 tatgaaacta gatgtttttg atgaagaaga taatctggtt ataacttgtg aaggggagac
     2101 ctctacaaaa tccgtggata cgctttcggg taattttgca gcgcgtcttg aggaagcttg
     2161 tgtagcggat gaatgcggta ctggtgtaag ggagtgtcat gttgaaaacg ttgatctaga
     2221 agaggttttt tgtggacctg aacctcaaga tgtagcgtgt cttgaacagc aggaggcttg
     2281 tgacgatgcg gtcgaggact tcgagtcaac aggaagtttt acgtatactg gcgatgttaa
     2341 agctacccat ataggtacag gcggcccaag tggcaatgca agttcatcag gtggaggcgg
     2401 aggcttagaa catcataagg ctatggcaga atgtccagaa ttctagaata gaaccttaaa
     2461 taaaagcaat tagttgttta ttgttaacaa aaaagcggtt agagtgtata tatcatcata
     2521 tcactctaac cgcttttaat attatttata ggaaatagat aagttacttc tttttttaaa
     2581 ttaacttgag aattccatat agcttctaga tgttttttct ctgttatcat gcgagcacat
     2641 tcgcggagcc gaagccctga gcaatgtgaa gggtcaggcg aaggaacagt gaggaatctc
     2701 aattagattg cttcgtcccc tttgacgtaa cttagggttc ctcgcaatgg cacttctgtg
     2761 ctttcattga aatgaacgaa gcggttgcaa gtttaaacgc tgatgccctg cagcttgcta
     2821 caggggtttt tcattgaaat attcatttgc ttataaatat tattggtagt tataataaga
     2881 ttgaagaaaa tttagaactt taagatcatt ttttagatta aagagtaaaa taatttaaaa
     2941 cgcgttgcta tgagttgcta tgaacagtgg tgaaagaacc gttgataaag ctggagatgt
     3001 cagttcagct tttactgttt ttgcttttct ttttgcctgg gcacaattat ttgactattc
     3061 ggcggctgta gccaccatct ttgatttttc aactaactat tatgacattg attattgttt
     3121 tacggtggct atttgcctga cagccgtatg ggtcatactt aatccttcat ccttatatag
     3181 atccattttc ttgtttgttt ttgtgattgt agggaccata attaggatgc caatatcccc
     3241 caatcatgta gtttttgagg ttattgtttt aatctctatt ttgacatgtt ttgtctatat
     3301 gaagctcaaa gagaaaagca gttttacaaa gacaaaattt tacgaagcat ttgcccctgt
     3361 agttagactt gaagttatcg ttctttattt ttgggctgca attcacaaga taaacacagg
     3421 attttttgat aaggatatca gttgcgcaac gattcagcta tttaatgtaa aagatgtatt
     3481 gcctattatg ccgactccgg attggtttat atctattaac ccatttttaa ccctcttaac
     3541 cgagagctca attccgatac tattaatcat ccccaaaact cgtattcttg gtttgattat
     3601 agccattatt ttccatttta ttcttggttt tgtatatact ggatttacta tccttgttta
     3661 ttccttttta tccttgttta ttcctgcatc tagctatgat cgaattaaac cgaattttaa
     3721 acaattaaca tataaactct ccaaaatact ttcaaagttc agtaattaca aggactggaa
     3781 aaagactcgg tttgataatt ttattactca acttattttt ctgatttcta ttttttttct
     3841 tatgggtttt tttatgagag gaaaccctga aaaattttac ctattgagtg aggaaggact
     3901 ttatattatt ttcttttttg tttttttgat cgccttcttc tattttgtta taagagggct
     3961 gaaggaattt aggatcgagc aaagaataac cctagtacct gaaatgaagt ggcttttaat
     4021 attcccaatt ttagttttag ttaatggatc tttaccccat ctcggggtga aaaatataca
     4081 gtctatggca atgttcagca atcttcgaac cgagggagga aagacaaatc atttatttat
     4141 tccttccttc tttcagtcgt ctaataacct agaagacctt gtcacaataa agggagcgaa
     4201 tttaagggaa ttaaataaat tttctggctt ctcatcaaga cgtcccttac gaggaacaag
     4261 cgttacactg cccaggtctt atgtcaaaca catggagaat aatgacaaag attatagaga
     4321 aaggtttgag tataaggttc cttttgtgtt acttcagaat ctggtaacgg gtttggcaaa
     4381 agagggtcaa aaggatatca aacttgaata tgagagagac ggggagatac tttatacaag
     4441 aaatgcagag gttgaccctg ttctaagcaa ggcatcaata tttcagagaa aatttatttc
     4501 acaacgagca gttcccgatg atgatagagg actctgcatg tggtgatatg ctatgacaaa
     4561 taaacatttt ttaaccaatg aaattgggct caggcatgca tattgactta tctccaatct
     4621 ctccctagaa tacttttatg gccaggaaac ccagaatcga acatctttat aaaagtggac
     4681 gccctttttt ctgatattag agatgaatcg aggtgaggta tgtggttgac attggcgtgt
     4741 atttttttta aaatcttata atgttagtac gtattaagaa atcctgcttt ttatctcttg
     4801 ctttaccttt tcttttagcc acatctgaat caaagtctgg taacggactc ccttttcctc
     4861 agctattttc tgaagctgtg ccttaagcct ctgatcaagc aacatagtta ttctttgttt
     4921 tctgacttta ggcttggtta cttctatcgg ctcactaatc tcctcggtct catcgaaaaa
     4981 ttctgtactg tcgtagctag accagaagtc aaattcctct tcttcgtttt taaatttagg
     5041 tatcttgctt ttatgctttt tcatagtttt gttgctctca ttttttttcg taatagcgtt
     5101 tttccttttg ggtcatatcc cttgcacaga ttcttcacaa tttactcttg ctaaagagca
     5161 gtttgactta agttacccat aaattcaaaa gcatattatc aactgtgtat ttaataataa
     5221 aaactcattt ttcaagcaaa ttcaccaaag gccttaaatc tctaaattca tagtctggtg
     5281 taatattacc ttttaccttt ttatcttttc tgttaatcca cactgcctga aagcccaatt
     5341 ctttagcagg aactatgtca tgaacgtagc ttgctgcgac atggagaaca ttttcctttg
     5401 gaatcttgaa aacatccatc attcgcttcc agtgaccgtg tgagggcttg taggatttca
     5461 cctgtt
//
LOCUS       DATDRL010000271         3687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_570368, whole genome shotgun sequence.
ACCESSION   DATDRL010000271 DATDRL010000000
VERSION     DATDRL010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570368"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VI935_09035"
     CDS             <1..265
                     /locus_tag="VI935_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25780.1"
                     /translation="GCIYGISRNYLEGGRNPYGGYNCDTMGSNKISNNYGGTIDRKIS
                     NSKAATLLGISRRQVIRIKNKVRRFDFKGIVHGNRGRSKEANR"
     gene            complement(447..971)
                     /locus_tag="VI935_09040"
     CDS             complement(447..971)
                     /locus_tag="VI935_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25781.1"
                     /translation="MFRRLSIRKIGKVGMKYNPDVHHRRSIRLKEYDYSQAGAYFITI
                     CTRNKELYFERYSKLKQIVNQQWQKILENYANIQLDEFVIMPNHIHGIIFVGATLAVA
                     QKNRAGARPAPTIAQNNRARSRPAPTIGEIIGTFKSLCVYDLLKYIKKTRLMRSVNSG
                     NEIIMKKLSVTNMN"
     gene            complement(950..2077)
                     /locus_tag="VI935_09045"
     CDS             complement(950..2077)
                     /locus_tag="VI935_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HLE25782.1"
                     /translation="MIYKNRTFLLGAGFSKAVADGPLLKDLWCYIEKAYKREKNRTDI
                     SQEDKKLRMDWYDYINEFITKLEKAAISRFRGRKFGHEYNPSVDMRENLEYVFTLIDL
                     HRDGPLVRFERKGADVQPYPAIPFQFITRDELDGVRRNLQTYIYLVLNRLKFNELAEK
                     FAEIIAPSDQIITFNYDLVLEKSLLHLDKWYPLNGYVGVHRFEIDSDGRDLEDQGKRS
                     KIQIHKMHGSINWRIPEPRSLERLQGINEVMIVMDDWENNGFHFDGSLQRQPDKTKSP
                     YVGRHEPESILPSFVKPFKEKEIFRIWQSAMRFMSNTKELVIIGYSFRPEDSNSFLLL
                     SMLPQTCDITLVDLYPEEIKERLENKGLKVARTFKCLEDYL"
     gene            complement(2203..3066)
                     /locus_tag="VI935_09050"
     CDS             complement(2203..3066)
                     /locus_tag="VI935_09050"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLE25783.1"
                     /translation="MQEELRIKKRHEMAKSFSVGDFMLRLYVFIFFLVLYSPIIIVVI
                     FSFNEAPFSYFPFKKFTLNWYREFLNNNDLMQAVFNSIKVGIAATVFSLIVGTLLALA
                     MDEFIFPLKGVFHRVSTWPITFPGVITGVALLLFCSFARFDLSLITVAIGHITFCIPV
                     VYLEVLARLKAMDKSMVLAAMDLGANRWRAFFSVVLPNITTALLGAALLAFTLSFDEI
                     IVTIFLTGRDNTLPMQIWAMLRRGMTPEINAVGTIILFVSITSLIIWERLRSRDIEHK
                     RLQIAKAQLRV"
     gene            complement(3258..>3687)
                     /locus_tag="VI935_09055"
     CDS             complement(3258..>3687)
                     /locus_tag="VI935_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLE25784.1"
                     /translation="PLSILLYSKFALILVLTMAFIAFLIIPVYAMLEKIPDEFLQASY
                     DLGAGRFTTFWRVIFPLTLPGVIAGITSVFALSVGDFVAALLVGGPEGIMIGNIVWSL
                     FGSAFQWTLGAANGLVLILLVAFILYISEKIAARYSGMEI"
BASE COUNT         1167 a          721 c          660 g         1139 t
ORIGIN      
        1 cgggtgtatt tatggtataa gcaggaatta tctagaagga ggtagaaatc cctatggagg
       61 gtataactgt gatacaatgg gaagcaacaa gatatcaaat aattatggcg ggaccataga
      121 taggaagatc tcaaatagta aagcagctac tttgcttgga atatcaagga ggcaagtgat
      181 aaggattaag aacaaagtaa ggaggtttga tttcaagggg atagtacatg gtaatagggg
      241 taggagtaaa gaggctaatc ggtgaggtag gaaaaagatt agaaagactt agaaattaac
      301 gcagaattaa ccgcttgtca ctacctcttt aagcgaggtc tgtctaaaaa tttcatcttc
      361 caatgcatct gtggcaattt tttctggatt ttctctatca agatgccatt ttaatggatt
      421 attttgaata tattccctga ttttgtttaa ttcatattcg ttacggataa ttttttcata
      481 ataatttcgt tgccagaatt taccgaccgc atcaatcttg tcttctttat gtattttaac
      541 aagtcatata cacacagtga tttaaacgtt ccaataattt caccaattgt aggggcgggt
      601 cttgaacgcg ccctattatt ttgggcaatt gtaggggcag gccttgcgcc tgccctattc
      661 ttttgggcaa ccgcaagggt tgcccctaca aatattatcc catgaatatg attcggcata
      721 attacgaatt cgtctaattg tatattggca taattctcta gtattttttg ccattgttga
      781 ttcacaattt gctttaattt tgagtatcgt tcaaaataca actctttatt acgtgtgcaa
      841 atcgttataa aatatgcacc tgcctgtgaa taatcgtatt ctttcaatcg tattgacctt
      901 ctatgatgaa catcaggatt gtatttcata ccaaccttgc ctatttttct tatagataat
      961 cttctaaaca tttaaaagtc cttgcaacct taagtccttt attttccagc ctttccttta
     1021 tttcttctgg ataaagatca acaagtgtta tatcgcatgt ctgaggtaac attgaaagta
     1081 aaagaaacga attagaatcc tctggtctaa aactgtatcc aataataaca agttcttttg
     1141 tgttagacat aaatctcatg gcagattgcc aaatccgaaa tatttctttt tctttaaagg
     1201 gcttaacgaa agacggcaat atcgattctg gttcatgtct tcctacataa ggtgatttag
     1261 ttttatcagg ttgcctttgt agcgaaccat caaagtgaaa accattattt tcccaatcat
     1321 ccataacaat cataacttca ttgatacctt gaagtctttc gagcgaacgt ggttcaggga
     1381 ttctccaatt aattgaacca tgcatcttat ggatttgaat tttggaacgc ttcccctggt
     1441 cttctaaatc tcttccgtct gaatctatct caaacctatg aacccccaca tagccattta
     1501 gaggatacca cttgtcgaga tgtagcagtg acttctccag aactaaatca taattaaacg
     1561 taataatttg gtcagaagga gcaataattt ctgcaaattt ttcagctaat tcattaaatt
     1621 tgagtctatt aagaacaagg tagatatatg tttgtaaatt tcttcggaca ccatccaatt
     1681 catctcttgt aataaattgg aatggtatcg caggataagg ctgaacatca gctccttttc
     1741 tttcaaaacg aacaagtgga ccatctctat gtaagtcaat tagggtaaat acatattcca
     1801 aattctctct catatcaaca ctagggttgt actcatgacc aaatttacgt cctctaaaac
     1861 gacttattgc tgctttttcc agtttagtta tgaattcatt aatatagtca taccaatcca
     1921 ttctaagctt tttatcttct tggctaatat ctgttctatt cttttctctt ttgtaagctt
     1981 tttcaatgta acaccacagg tcttttaaca aaggaccatc agccacggcc ttggaaaatc
     2041 ccgcacctaa caaaaaagtt ctgtttttat atatcatgta gacactaaat cgttcagttt
     2101 tatgttaata gagaaggcca gaagtagcaa acggggggca tattagaaat tttatgaaag
     2161 actaagaaaa cttggcgaat ataattcaaa tctatagcta aatcacaccc tcagttgggc
     2221 ttttgctatt tggagtcgtt tatgctctat atcccttgac ctcaatcttt cccaaataat
     2281 aaggcttgtt atcgagacaa agagtattat agttcctaca gcatttatct ctggggtcat
     2341 cccccttcga agcatggccc aaatttgcat aggaagtgtg ttatcccttc ctgtaagaaa
     2401 gattgttacg atgatttcat caaaagataa agtaaatgca agaagagctg cgccaagaag
     2461 agcagttgtt atattcggaa gaactacgct gaaaaatgct ctccatctat ttgccccaag
     2521 gtccattgcc gcaaggacca tagatttatc cattgctttt agacgtgcta aaacctctaa
     2581 ataaactact ggtatacaga atgtgatgtg tcctattgcc actgtaatca aagaaagatc
     2641 aaaccttgca aacgagcaaa aaagaagaag tgcaacacct gttatgacac ctgggaaagt
     2701 tatcggccat gtggaaactc tatgaaaaac gccttttagt gggaatatga actcatccat
     2761 tgcaagagca agaagggttc ccacaatcag agaaaaaaca gttgcagcaa ttcccacttt
     2821 gatgctgtta aagaccgcct gcatcagatc gttattattt agaaactccc tataccaatt
     2881 aagagtaaat ttcttaaatg ggaaataact gaaaggggct tcgttaaatg aaaatataac
     2941 tacaataata atgggagagt aaagcactag gaagaagata aacacataga gcctgagcat
     3001 aaaatctcct acgctaaatg attttgccat ttcatgtctt ttcttaatcc ttaattcttc
     3061 ctgcataaga ttaatccatc tccatatttt cactcgtaac gatttggcca ctaatgaaca
     3121 cgaattcaca caaaagtgat gcacgatcca tgatggtttt taattacaga ttatcctgca
     3181 tcccgtatcg tgtatctagc atgttgttta ttttctttgg gctcatcgta tcaatccatg
     3241 gcgaattccg cctatgttca tatttccatt ccgctataac gtgcggcaat cttctcgctt
     3301 atataaagta tgaaagccac gagaagtatc aggacaagac catttgccgc tccaagcgtc
     3361 cactgaaacg cgcttccaaa aagactccaa acgatatttc caatcataat tccctcagga
     3421 cccccgacca aaagggctgc gacgaagtca ccgacgctga gagcaaacac cgaggttata
     3481 cctgctatga cacctggaag ggtcagagga aagataacgc gccagaaggt tgtaaacctc
     3541 cccgccccga gatcatatga agcctgaaga aattcgtcgg ggattttttc aagcattgca
     3601 tacactggga tgattaaaaa ggctataaaa gccatagtaa gcactaaaat tagggcaaac
     3661 ttgctgtaaa ggagaatact aagaggt
//
LOCUS       DATDRL010000272         8056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2728017, whole genome shotgun sequence.
ACCESSION   DATDRL010000272 DATDRL010000000
VERSION     DATDRL010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8056
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2728017"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VI935_09060"
     CDS             complement(<1..596)
                     /locus_tag="VI935_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HLE25785.1"
                     /translation="MFIRATTFFLGVLATLFLGWAGLTLIPGIQIREVKPSSELKPYT
                     EEQLRGRAIYIREGCIYCHSQQTRPEGFGADQLRNWGRPSLPGDYVYDKPHLLGTMRT
                     GPDLFNIAARQPSENWHLIHLYNPRTLSPGSLMPPYLWLFKEEDEFSKEVYAATGERV
                     VPVPKGFVPEGKVIIATQEALDLVAYLLSLNHTYPIKA"
     gene            complement(596..2035)
                     /locus_tag="VI935_09065"
     CDS             complement(596..2035)
                     /locus_tag="VI935_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HLE25786.1"
                     /translation="MSPDRNPFNIEESPELSSRVLSDISASGPVLAFFTSAVAWLLVG
                     TVFGLIASLKFNLPDWLGSFPALTFGRVRPVHLNTIMYGWVSLASAGCSIWLTVRLCR
                     VEVHWRNLLYPSVFLWNLGLLYGTIAILIGDNRGLEWLEFPRGASLLIAIAFVCLSLS
                     ILRTFHARQVKHIYVSLWYILASIVWFPILYVIANLPFYSGVTQAAVNWWFAHNAITV
                     WFTPIGLAAAYYFIPKVIGRPIYSYYLSLLGFWSFALFYNWNGIHHLVGGPLPTWLIS
                     VSIVASVLMFIPVTTVAINHHLTTIGHFGKLKFSPTLRFVVFGAMCYTAVSFQGSIEA
                     LRSFNEISHFTHYTIGHAHLGMYAFATMILFGAIYYIMPRLTKWEWPYPILIKYHFWL
                     VAFGIALYVGSMTVGGVLQGLYMNDPSRPFIASVEVTKPYLLARSGAGLLILIGHLIF
                     IYLFSLMILRRGPERKAPPWREEVEVVAP"
     gene            complement(2019..2258)
                     /gene="ccoS"
                     /locus_tag="VI935_09070"
     CDS             complement(2019..2258)
                     /gene="ccoS"
                     /locus_tag="VI935_09070"
                     /inference="COORDINATES: protein motif:HMM:NF015554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HLE25787.1"
                     /translation="MSLPLAGLILILIALIGGTMAMVAFIWAVRTKQFKDLNAGAYVI
                     FDEEEPVGKMTDNTFGFPESEKSSRKGKGKYESRP"
     gene            complement(2325..4412)
                     /locus_tag="VI935_09075"
     CDS             complement(2325..4412)
                     /locus_tag="VI935_09075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HLE25788.1"
                     /translation="MNHSKDSCYQCGLPLPRRPILGKVMGKDASYCCYGCLLAHEITG
                     EKGEEGEAMWIFARLGLSAFFAMNVMMLSYTDYFYPFEKNVSLAINYIMFALATPVMF
                     LLGTPILRNSIRGISKLTLNMDTLIVLGTFSAYVLSAISTFRAQGKVYFDTASMLLVL
                     VTVGRFLEAGAKARTSESIKELLNRSPKEAKVIRDGVEEVVPAEFLKKGEIIKIIPGE
                     NFPADGKVIEGEGSVDESMLTGESKPVFKEKGSMVSAGTVNLDGRLIFKATSVGEEMA
                     LSRLCSLLEEARKSRAPIERFTDRVSSFFIPLTVIAAVVTFIVWNFKSGIDTALMNSL
                     SVLLISCPCALGLATPMAIWVSLGRAAKEGILIRTGETLEKLSRINKILFDKTGTLTK
                     GKMELTSVFVDSDSRISESDLVSISASLESNSEHPLGKSLVEFANKRGYAILPIYQFK
                     ASPGMGVQGRVGELGETVYIGSRRFMEKSGLEFTKNILEEKTKEETRGKTAVFCGWGK
                     RIKGILGFSEELREEAVDTISSLKGLGVEIYIITGDDKYASEALSKGLGIEAKSELLP
                     EDKVNVIKALKKTRGLTAMVGDGINDAPALASADVGIALGCGVDITRESADVSLLGND
                     LKKIPWVLQLAKKTKRKIKENLFWAFFYNTIGIGFAISGTLKPLIAAIAMILSSLFVL
                     GNSLRLQKIKIND"
     gene            complement(4561..5256)
                     /locus_tag="VI935_09080"
     CDS             complement(4561..5256)
                     /locus_tag="VI935_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLE25789.1"
                     /translation="MIEYVLFFIAGIAGSFHCIGMCGGFPIAVGSVSNRSPSQKVLNQ
                     LLYNSGRIFTYTFLGILLGYFSLKINEIQPILNAQVIVSVIAGIFMILIGLQILGMLG
                     ETAVPGFTPIYIFIKKALASFIGYKSLVAPFYLGMFNGFLPCPLIYAFLFKAATSGSP
                     DKGGMTMLSLGLGTIPTMFLLGSVSGFISPRIRAKVSLIPGLVVLIFGIITIIRALLP
                     LNFLEYHQVHFYH"
     gene            5728..5802
                     /locus_tag="VI935_09085"
     tRNA            5728..5802
                     /locus_tag="VI935_09085"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5760..5762,aa:Met,seq:cat)
     gene            complement(6025..6366)
                     /locus_tag="VI935_09090"
     CDS             complement(6025..6366)
                     /locus_tag="VI935_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25790.1"
                     /translation="MYSINRSIAIIRPKQPFVEWANQLPDADFKVSVDELQNDCLVIL
                     IPEYTTNKEAKECINELYEDIFEEELSGWCTNESWWPKKRTQEMFWQWFNVEFHSVVM
                     DPNGEPIEKER"
     gene            complement(6511..6882)
                     /locus_tag="VI935_09095"
     CDS             complement(6511..6882)
                     /locus_tag="VI935_09095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015079777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HLE25791.1"
                     /translation="MAKKQKVAFPQRGEIWLVDFEPTRGAEIKKTRPALILQNDIANH
                     YSPITIVAALTSQFEEPLYPTEVLVKASEGGLEADSVVLLNQIRSIDKKRLIKRIGVL
                     KLETMIRINRALQVSLGLVKI"
     gene            complement(6861..7106)
                     /locus_tag="VI935_09100"
     CDS             complement(6861..7106)
                     /locus_tag="VI935_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015210701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25792.1"
                     /translation="MHRRVNITLPEETIRLISRVTGKGDRSRFIDKAVKHYIEEIGKA
                     NLRKQLKEGAIQRAERDLGLAHEWFLIEEETWQKSKK"
     gene            complement(7602..7733)
                     /locus_tag="VI935_09105"
     CDS             complement(7602..7733)
                     /locus_tag="VI935_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25793.1"
                     /translation="MFDNHFYNLMAQIVEENKSLWRIKNMYKKDASGCKQCQTFWDK"
BASE COUNT         2401 a         1735 c         1556 g         2364 t
ORIGIN      
        1 tctgctttaa tcggatacgt atgattaaga cttaaaagat aagccactaa atcaagggct
       61 tcttgtgtgg ctattatgac cttaccttca ggaacaaaac cttttggtac aggaacaact
      121 ctttctcctg ttgccgcata cacctctttt gaaaattcat cctcttcttt gaagagccac
      181 agatagggtg gcatcaacga accgggagaa agtgttcttg gattgtatag gtgaatcaaa
      241 tgccagtttt cgctcggctg acgggctgca atgttaaaaa ggtcgggacc cgttctcatc
      301 gtcccaagta gatgtggttt gtcataaacg taatcccctg gtagggaagg ccttccccag
      361 ttacgtagct ggtctgctcc gaacccttcg ggacgagtct gctggctatg gcagtaaata
      421 caaccctcac gtatgtaaat ggcccttcca cggagttgtt cttctgtata gggctttaat
      481 tccgaagacg gttttacctc gcggatttga attcccggaa tcagtgtcaa gccagcccaa
      541 cccaaaaaga gggtagccag cacccctaaa aagaaggtgg tagctctgat aaacatcatg
      601 gggcaactac ctccacttct tctcgccaag gtggagcctt tcgttcaggt ccccttctta
      661 gaatcattag ggagaacaag taaataaaaa tcaaatgtcc aatcagaata agcaagcccg
      721 cccccgaccg agctaaaaga tacggcttag ttacctccac cgatgctata aaaggtcggg
      781 aggggtcatt catatatagc ccctgtaaaa ctcccccgac ggtcatagac cctacataca
      841 acgcgatgcc gaaagcaaca agccagaagt gatactttat aagaatcgga taaggccatt
      901 cccacttggt cagcctaggc ataatatagt agatcgcccc aaaaagaatc atagtggcga
      961 aggcatacat cccaaggtgt gcatgaccga ttgtgtaatg agtaaaatga gaaatttcgt
     1021 taaacgagcg caaggcctct atagagccct gaaaactcac cgctgtgtag cacattgcgc
     1081 caaaaaccac aaaacgtaag gttggactaa acttaagctt tccaaaatgg cctatcgtgg
     1141 ttaggtggtg attaatcgct acagtagtta ccggaatgaa cataagaaca cttgccacaa
     1201 tagagacgct tatgagccag gtaggaagcg gcccgccaac cagatgatga ataccattcc
     1261 agttgtaaaa aagagcaaat gaccagaagc cgagaagcga gagatagtag ctataaatcg
     1321 ggcgaccaat caccttggga ataaagtagt aagcggcagc caaaccgatc ggtgtgaacc
     1381 aaactgtaat tgcattgtga gcaaaccacc agttaaccgc tgcttgagta accccggaat
     1441 aaaagggtaa gttagcaata acatagagaa tagggaacca tacaatagat gccagtatgt
     1501 accagaggga aacgtatata tgttttactt gacgtgcatg gaaggtgcga agtatagaga
     1561 gcgagagaca aacaaaggct atggcaatca ggagggaagc accccgcgga aactctagcc
     1621 actccaatcc ccgattgtcc cctattagga tggcaatcgt tccatataag agccccagat
     1681 tccaaaggaa gacagaaggg tataaaagat tgcgccaatg gacttccacc cggcagagtc
     1741 gaaccgtgag ccaaatagag cagcctgccg aagctagaga aacccagcca tacattatgg
     1801 tatttaggtg aacaggacgc acacgtccaa aggtcaaagc tgggaaagaa cctaaccaat
     1861 cgggaaggtt gaatttcaag gaagcaataa ggccaaaaac ggttcctaca agtagccacg
     1921 ctacagcaga tgtaaaaaag gcgagcaccg gacccgaggc ggaaatatca cttagcacgc
     1981 gggaggataa ttcaggagat tcctctatgt taaagggatt acggtctgga ctcatacttt
     2041 cctttcccct ttctgcttga tttctctgat tcagggaatc caaaagtatt atcagtcatt
     2101 tttccaactg gttcctcttc atcaaaaata acatatgcac cagcgtttag gtccttaaac
     2161 tgttttgtac gcacagccca gataaaagca accattgcca ttgttccacc tataagggca
     2221 attagaatta aaataagacc agctagagga aggctcattt ctacacctga ttgttataag
     2281 ttgcacttaa aattacaaca tatcatgatt agttacaata cacatcagtc attaattttt
     2341 attttttgaa gtctaaggga atttcccaag acaaaaagac tgcttaaaat catggcaatg
     2401 gctgcaatca gaggtttcaa agttcctgaa atagcaaatc caattccaat tgtgttgtaa
     2461 aagaaggccc agaaaagatt ctcttttatt ttcctcttag tttttttagc aagttgcaaa
     2521 acccatggga ttttttttaa atcattccct agcaaactca catctgctga ttcacgagtt
     2581 atgtctacac cacatccaag ggctatgccc acatctgctg aagcaagcgc aggggcgtca
     2641 tttattccgt ctccaaccat cgcagtaagg ccccttgttt tttttaaggc tttgattacg
     2701 tttaccttat cctctggaag gagttcactt tttgcctcaa ttccgagacc ctttgataaa
     2761 gcctccgatg cgtatttatc gtctcctgtt attatataaa tttctacgcc taaacccttt
     2821 aaactagaaa tcgtatccac tgcttcttct cttagctctt cagaaaatcc taatatgccc
     2881 tttattcttt ttccccaacc acagaaaaca gcggtttttc ctctagtttc ttctttagtt
     2941 ttttcttcga ggatattttt agtaaattct aatccgcttt tctccataaa tcttctactg
     3001 cctatgtata cagtttcacc aagctctccg acccttcctt gaactcccat tccgggactt
     3061 gctttaaatt gatatattgg gagaatggca taacctcttt tattagcgaa ctcaaccaaa
     3121 ctcttcccaa gcggatgctc agaattagac tctaaggagg cgctaattga taccaggtct
     3181 gattcgctga ttcttgaatc tgagtcaaca aaaaccgacg taagctccat ttttcccttt
     3241 gtaagggtac cggtcttatc aaatagtatt ttatttatac gagaaagctt ttccagggtt
     3301 tctccagttc ttataaggat tccttctttc gctgcccttc caagactaac ccagattgcc
     3361 attggagttg caagccctag cgcgcatggg caagagataa gtagaacaga aagtgaattc
     3421 atgagtgctg tatcaatgcc agatttgaaa ttccaaacaa taaaggttac tactgcggct
     3481 atcacagtaa gaggtataaa aaatgaggaa accctatccg taaatctttc tatcggcgcc
     3541 ctggattttc ttgcttcttc aaggaggcta cagagtctag atagggccat ttcctcacct
     3601 acactggtcg ccttgaatat cagccttcca tctagattca ctgtgcctgc agaaaccatg
     3661 ctaccttttt ctttaaaaac gggttttgat tctcctgtta acatagattc gtctacacta
     3721 ccttctcctt caattacttt tccatctgca ggaaaattct caccaggaat tattttgata
     3781 atttctccct ttttaaggaa ttctgcagga accacttctt caactccgtc tcttataacc
     3841 tttgcctctt tcgggctgcg atttaaaagc tcttttattg attccgatgt cctggctttt
     3901 gcaccagcct caagaaacct ccctacagtt acgagaacta gaagcatcga ggcggtgtca
     3961 aagtaaacct ttccttgggc tctaaacgta gagatagcag aaagaacata agcagaaaac
     4021 gttccaagaa caatcaatgt atccatgttt aatgtgagtt ttgaaattcc tcttatagaa
     4081 ttcctaagaa tcggagttcc aagaagaaac atcacgggag tggcaagggc aaacataatg
     4141 taattgattg ccaaagaaac gtttttttca aaaggataga agtaatctgt atagctcagc
     4201 atcatgacgt tcatcgcaaa aaatgcgcta agcccaagcc ttgcaaatat ccacatggct
     4261 tccccctctt cccctttttc tccagtaatt tcatgggcga gcaagcaccc ataacaacag
     4321 tatgaggcat cttttcccat cacctttcca agaataggcc ttcgggggag aggaagtcca
     4381 cactgatagc aagaatcttt tgaatgattc atttgtctga atgtctaacc gctactgaaa
     4441 tctacaatga ccgggtatcg aatttagaag atttgttact tggaatatgg agtatgatca
     4501 gttttcattt atctaaattc gtgaccgaat atctatagcc cttatcaatc gtatcctagt
     4561 ttaatggtaa aaatgaacct ggtggtactc aagaaaatta agcggtaata atgcgcgaat
     4621 aattgttatt attccaaata taagtactac taatcctggt attaggctta cttttgctct
     4681 gattcttggg gaaataaatc cgctcacgct tcctaaaaga aacatagtgg gaattgttcc
     4741 aagtccgaga gatagcatag tcattcctcc tttgtctggc gaacccgatg tcgccgcttt
     4801 gaacagaaat gcgtaaataa gcgggcaggg tagaaacccg ttgaacatcc cgaggtaaaa
     4861 aggggcgact aagcttttat atccgataaa agaagccaga gcttttttaa taaagatata
     4921 tataggggta aaacctggaa ctgctgtttc gccaagcatt ccgagtatct gaagtcctat
     4981 caagatcata aaaatacctg caataactga aacgataacc tgtgcattaa gaattggttg
     5041 tatctcatta atcttgagac tgaaataccc gagtaaaatt cctaaaaagg tataagtaaa
     5101 aattcttcca gagttgtaaa gaagctgatt taaaaccttt tgtgaaggac ttcggttaga
     5161 aactgatccc actgcgatag gaaaaccgcc acacattcca atacaatgaa atgagcctgc
     5221 tataccagca ataaaaaaaa gtacatactc tatcatggaa aaaaaccaac ccgcacgtaa
     5281 tccatttgag tattatctaa aagaatataa taatccaaaa cacccactta attctagatg
     5341 agatggaata ttaaattaaa agtgaaaatg atagtccaag agtaatgaga gcgggctata
     5401 aaaaaattaa gtgggaatgc ccctttcgga acattcccac tctcttaaag gaggatggag
     5461 gatggcttta atttaagtgt ttcaattaat atctattatt aaaataaagt atattactta
     5521 atttgtcaag gggtaatttt tcaaatttaa aaattctttt aaaagtggct tcgagcaaac
     5581 ctcacgccct caatgtatgg gtatctatcc gttgaaagcg aagcaaaaca tgatataatt
     5641 tgcgttcatt atatatttag agcaagtccc tagaaaatag attcagggta tatggtaaat
     5701 acctaactta ttgaaatcat tagatttggc ggcgtagctc agaggtagag cagggctctc
     5761 ataaggcctt tgtcggaggt tcgattcctc ccgccgccac caccaaaaat cccttttatt
     5821 taaagtggtt acgtagagta tgcctctgtc ggaatcgtcg ctacatccta taggggtgtc
     5881 caaggaggtg tctatacata ctagttccac agcctttttc ttatgctccg gtgttaggta
     5941 tgagtaccgt ttcgtcatgg ctaatgtttt gtgtcctaaa agtttctgcc tgttactgaa
     6001 tgtaccttga actatagatg cactctacct ttctttctca atcggctccc cattgggatc
     6061 catcacaact gaatggaatt caacattaaa ccattgccaa aacatttctt gagttctttt
     6121 ctttggccac cacgactcat ttgtgcacca gccagacagc tcctcttcga aaatgtcttc
     6181 atagagttca tttatgcatt cttttgcctc tttattagtt gtatactctg gaataagaat
     6241 gacgaggcaa tcattttgta gttcatctac ggatactttg aaatctgcat caggaagctg
     6301 atttgcccat tcgacaaatg gctgtttggg tctaatgatt gcgatggatc gattaatact
     6361 atacattgtc tcgtaaataa ttgtatatca tttccggaga taggagtact tatgaaattg
     6421 cgaggttata gaatataacc gaatgagaga taataaaaag gaagctcatc cggtacttct
     6481 gcatcaaatc tcattctcct agtgattcta ttatattttc accagcccga ggctaacctg
     6541 aagtgctcga ttgatacgga tcatagtttc tagtttcaag acaccaatac gtttgatgag
     6601 tcgtttctta tcaatcgaac ggatttgatt gaggagaaca actgaatcag cttctaaacc
     6661 tccttcagaa gctttcacaa gtacttcggt aggatagagc ggctcttcaa attgagaggt
     6721 gagggcggca acgatagtga tgggactgta atggttggcg atatcgttct gtaagataag
     6781 ggcgggtcgc gtcttcttaa tttccgcgcc ccgcgttggt tcaaaatcca ccaaccagat
     6841 ctcgcccctt tgaggaaagg ctactttttg cttttttgcc acgtttcttc ttctatcaag
     6901 aaccattcgt gagcgagtcc gagatcgcgt tctgctctct gaatagctcc ttcttttagt
     6961 tgcttcctga gattcgcttt cccgatctct tcgatatagt gtttcaccgc cttatcaatg
     7021 aagcggcttc gatcaccttt tcctgtcacg cggctaatca acctaattgt ctcttccggg
     7081 agggttatat taacccgacg atgcattgtg atatgacctc cattcatgat agatttaata
     7141 tacacatata tagtgcgtat atcaaataat gaagagtacg acagtttcta agctggaatc
     7201 tataaaccga atccctgctg atggggtcta tggacagggg tggttagtaa cctcacagta
     7261 gcgaagtatc atatctttgt aaagtctttg taaggggacg actattgata tacattaaat
     7321 gtttaactca atttgtagtt agcttaacgc tcaatatata gtcgggctta attgatttcg
     7381 gcaatcaata agaagtcaat atgcatgcct gaccccgcta actactcttg caaaataatc
     7441 tcattaactt gcctcctatg atatccataa ataaagtggt agttttattt tagcttttga
     7501 aactccccat tttgagacta atctttttat acttatcatt tgttaatgtg tgttttgata
     7561 attacctcga gttctctgat atgatcctct ttatcttttt cttacttgtc ccagaaggtt
     7621 tgacattgct tacagcctga agcgtctttt ttatacatat ttttgatacg ccagaggctc
     7681 ttgttttctt cgactatttg tgccatgagg ttgtaaaagt ggttgtcaaa catatttttt
     7741 acctcctttc cctttcattc ttatttttct taatcccatt ttatgcagaa ccctttccta
     7801 ctattaccat tttctcacaa gtaagaaata cttgtcaagc aaatttatat gtgtcaagcc
     7861 cccgtatata ggtggacttt tatacggggc ttgacaactt ctcctaaaat tgatgctgtc
     7921 aatacaaagt gaatatgtca cccctgatgc aaagtaaaaa tgtcacctgt aagtagattc
     7981 tcaaggggta gagtatgagt tttaatcttt ttagtttcac cagaactaat ctccttaaaa
     8041 ttcagatatt gatctt
//
LOCUS       DATDRL010000273         3073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1807081, whole genome shotgun sequence.
ACCESSION   DATDRL010000273 DATDRL010000000
VERSION     DATDRL010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807081"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(611..1339)
                     /locus_tag="VI935_09110"
     CDS             complement(611..1339)
                     /locus_tag="VI935_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HLE25794.1"
                     /translation="MKSKNMKVLVLRHVPHEHLGTLAETLISNDITHEYLNFYENNIS
                     NISVDDLASLIILGGPMNVYEADRYPYLEMEDRLIKQAIEKDVPILGVCLGAQLIAKA
                     LGSRVIKNKEKEIGWYPLNITEEGSKDRLFKHFNSEETVFQWHGDAFEIPEGGVRLAE
                     SSLCTNQAFRYGHNVYGLQFHIEVTPEMILEWLNVPENQKEILSLEEKINTEIIKAET
                     PNFIGRLNLLAKDVFQEFCGLVRK"
     gene            complement(1471..2646)
                     /locus_tag="VI935_09115"
     CDS             complement(1471..2646)
                     /locus_tag="VI935_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLE25795.1"
                     /translation="MAIPLKYNIRNLFVRKITTGLTVLGIGLVVAVFLSVMSLSEGLT
                     RVFKASGSTRNVLVLRQNSQSEVQSGISRNQVPLIVTLPGIEKDIDGNPLTSTELVVM
                     LNLKKIDGGASNVTLRGISEKSPLLKPQFKLIEGRMFRPGLSEVVVSRSISERFKDCR
                     LGDTIRFGVYKWKVVGIFDAGGTAPDSEIWTDVEGAMNSFKRQSYSSVLVRTTDKAAR
                     DQFIAALAVDPRLALEGKVERAYYDKQTSTADPIKFFGILIGIIMAIGAGFGAMNTMY
                     AAVSTRTAEIATLRVLGFSRLAILTSFIVEALCLALLGGLLGCLFGILAVELALSGVT
                     GTTNFTTFSEVVFAFRLTPKILLTGIILSLFIGLFGGILPATQAAFTKIILALRQIE"
     gene            complement(2650..>3073)
                     /locus_tag="VI935_09120"
     CDS             complement(2650..>3073)
                     /locus_tag="VI935_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLE25796.1"
                     /translation="MLGNVKLLITSLGTIVVFVILLIAANTMALSARERIREIALLRT
                     LGFSQVTILMLTLGESLMLALFGGILGLGLFVLGFRPLKASLINTPLSGFATAMTLFP
                     EVLLLGFVITLFVGIFSGLVPAIQASQRPITEGLRHIG"
BASE COUNT          893 a          670 c          636 g          874 t
ORIGIN      
        1 gccttgcctc ttcctctagc tttcgatatt gctccggatc aaaaagtatc attgcctttt
       61 tagtaaacgt tggcatacgt aaatgtcctt atatcagtat ctagatatag atactaacat
      121 tcctgatcag tccatgtcaa gtgtggcgct acctatgcac attattttgc ctaacgcagg
      181 cagccaggct acatctgctg gtaaataaag gggaacgcgc caccttttta tttcctttat
      241 ttaaaagtga gtttgggaca tttgattcat ctcaagtatg ggcaattcat gaattgccca
      301 tacttaacaa aacgttcata aaagtacgac gcaacccttt tttctttttg tcgataaatg
      361 tagggtctga tgccctcatc agtccacatg ttattgctcg ttcggagaac ggtcgctact
      421 taaccaaagt ccttgaagga ttctttatgt aacccatgcc ttctcttctt tcgatagagc
      481 ttcttgttaa aaagtttttc ggtgggttta tctctgtggg gttagagaat gtagaatgtt
      541 gatttcagag ccattggaat ttaaagttgt taataagcta catcgtcaaa taagcaggtg
      601 atttaaaatt ctatttccta actagtccac aaaattcctg aaaaacatcc ttcgccagca
      661 aattaagacg acctataaaa ttaggagttt cagccttgat aatttctgta ttaatctttt
      721 cttctaaaga gaggatctcc ttttgatttt ctggtacatt cagccattct aaaatcattt
      781 cgggtgtgac ttctatgtga aattgaagtc catatacatt atgcccatat ctaaaagcct
      841 gatttgtgca gagacttgac tctgccagac gaactccacc ttcgggtatt tcaaatgcgt
      901 ctccatgcca ctgaaaaaca gtttcctcag aattaaaatg tttaaatagt ctatctttgc
      961 ttccctcttc agtgatgtta agtggatacc agcctatttc cttctctttg ttctttataa
     1021 ctctagaccc tagcgccttt gcaataagtt gtgcgccaag acagacgcct aggattggaa
     1081 catctttttc tatcgcttgc ttgattagtc tatcttccat ttccaaatat ggataccgat
     1141 cagcttcata aacattcatc gggccaccta gaatgatcag gctggctaaa tcgtcaaccg
     1201 atatattcga aatattattc tcatagaagt ttagatactc atgagtaata tcgtttgata
     1261 taagtgtttc agcaagtgtt cctaagtgtt catgtggaac gtgtcttaga accagaacct
     1321 tcatgttttt gcttttcatt cctgtctaat attatatacc agtattgtat aaaagtagtc
     1381 atcaggagta agaacgtatt gcaatacgcc cctacaatca aagatcgctc tctttcacaa
     1441 aggttcacta cacaatccaa cttaccagtt ttactctatc tgacgaagag ccaggattat
     1501 tttagtaaac gctgcctggg tagccggcaa tataccacca aataggccta taaatagtga
     1561 cagaataatt ccggtaagaa gaattttagg cgtcaaacga aatgcaaaaa caacctcaga
     1621 gaaggtggta aaattggttg tgccagtcac tcctgaaagt gctaattcaa cagcaaggat
     1681 cccaaatagg catccgagaa ggcctcctaa tagagccaga caaagtgcct caacgataaa
     1741 cgaggttaag atagccaagc gtgaaaagcc gaggacgcgt agcgtggcaa tttccgctgt
     1801 acgagtagaa acagcagcat acatcgtatt catagcgcca aaaccagctc cgatagccat
     1861 tatgattcct attaaaatac cgaagaactt aattgggtca gcggtagatg tctgtttgtc
     1921 gtagtaggca cgctcaacct ttccttcgag agcaaggcgg gggtctacgg ctagggctgc
     1981 aataaactgg tctcgtgcag ccttatctgt tgttcgaact agaaccgatg agtaactctg
     2041 tctcttgaag ctattcatag ccccttccac atctgtccag atttcactgt caggggcggt
     2101 cccgccggcg tcaaagattc caactacctt ccatttgtaa acaccaaagc gaatagtatc
     2161 gccgagacgg caatctttaa accgctccga gatacttcga gaaactacga cctctgagag
     2221 acctggcctg aacatacgtc cttcaatcag tttgaattgc ggttttagaa ggggactctt
     2281 ttctgaaatt cctctaagcg tgacgtttga ggctcctcca tcaatctttt tcaggttgag
     2341 cataacgact agctctgtag aggttaaggg gtttccatct atatctttct caatcccggg
     2401 tagagtgacg ataagaggga cctggttgcg ggaaatgcct gattgaactt ccgattgaga
     2461 attttggcga agaactaaaa cgtttcttgt cgagccggat gccttgaaga cgcgagttaa
     2521 accctcggaa agtgacatga cagagaggaa gaccgctaca acaaggccaa tgcctagaac
     2581 cgtcaagccc gttgtgatct tacgaacaaa gagattccga atgttgtatt tgagaggaat
     2641 agccattttt cagcctatgt gtctaagtcc ttctgtaatg gggcgctgac tagcttgaat
     2701 tgcaggaaca agccctgaga aaataccaac gaaaagtgta atcacgaaac caagcaagag
     2761 aacttctgga aaaagggtca tggcggtggc aaagccggaa agaggggtgt tgatcaaact
     2821 tgccttgagt ggtctaaagc ctaaaacaaa gagtcctaag cctaggatgc ctccaaaaag
     2881 cgcaagcata aggctctccc ccagggtcag catcagaatt gttacctgac taaagcccaa
     2941 cgtgcgtagc aaagcaatct ctctaatacg ttcacgggcc gagagtgcca tggtgtttgc
     3001 agcgatcaaa aggatgacaa agacaacgat ggttcctaag gatgtgatta agagctttac
     3061 attgccaagc atg
//
LOCUS       DATDRL010000274         5520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1393211, whole genome shotgun sequence.
ACCESSION   DATDRL010000274 DATDRL010000000
VERSION     DATDRL010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393211"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="VI935_09125"
     CDS             complement(<1..1583)
                     /locus_tag="VI935_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HLE25797.1"
                     /translation="MSFNGKELEKIKEKTKIWENEYKNIPKREVKFTTVSGMDVPPLA
                     TPNSLEDFDYLRDLGFPGSYPFTRGIYNTMYQGRLWTMRQFAGFGSPEDTNRRFKFLL
                     EQGQTGLSTAFDMPTLMGYDCDHPRASGEVGKEGVAVSSLADMEVLFDGIRLDEVTTS
                     MTINCSASIILAMYLVLAEKQGVSWEKLDGTIQNDMLKEFIAQREWICPPRPSVKIVV
                     DMIEFCAKWVPKWHPVSISGYHIREAGSTAIQELAFTLADGIGYVQECIERGLNVDDF
                     APQLSFFFNVHNDFLEEVAKLRASRRIWAKIMRERFGAKNPKSWMLRTHAQTAGCSLT
                     AQQPLNNIVRVSIQALAAVLGGAQSLHTNSMDETLALPTEESVTVALRTQQIIAEESG
                     VANSIDPLCGSYVIEALTNRIEKEALDYIRRIDELGGIIKAIDIGFPQKEIADAAYRY
                     QLELDRGEKVIVGVNKYLNDNEKPMQLLKIDEEVDNNQRERLREIKRKRDNEAVKKHL
                     GAIAEAAREGKNLMPFIINA"
     gene            complement(1666..2817)
                     /locus_tag="VI935_09130"
     CDS             complement(1666..2817)
                     /locus_tag="VI935_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HLE25798.1"
                     /translation="MSIIELTDEQQMMRDVARDFAQNEIKPLASELDREGRFPAELVK
                     KMAELGFMGIFIPEEYGGGGMDTFSYVLALEEICKACASTGVVMSVNNSLVADPIYRF
                     GTEEQKKKYLLPLAKGEKLGCFSLSEPAAGSDAGSITTTAVKNREYYIVNGTKNWVTN
                     GPEADVIILFTSTDLSKRHQAITAFIVDKETPGIIVGKVEHKLGIRASSTSQLIFDGC
                     RVPIENRLGEEGLGFKIAMQTLDGGRIGIGSQAVGIAQAALDEAVSYSKEREAFSQPI
                     YNFQGIQFMLADMATRIEASRLLVWQAARMKDRKMKFSKQAAMAKLFASETAMWVTTK
                     AIQIHGGYGYTTDYPAERHFRDAKITEIYEGTSEIQRIVIANQVLKEYS"
     gene            complement(2907..4346)
                     /locus_tag="VI935_09135"
     CDS             complement(2907..4346)
                     /locus_tag="VI935_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLE25799.1"
                     /translation="MAKEYGFLVNGKWLKNAKKREVRSPYNNEVVGVINIPSLEEAKG
                     AIDAAEKAFGKFRNSPSYERSDILMRIVSGIEKRKEEFAKALAEEGGKPIKTARVEVS
                     RAMTTFKVAAEEANKFAGGELIPIDIVPGNEERFGIVKRFPLGIVMGISPFNFPLNLV
                     AHKVAPAIASGNVMILKPASQTPLSALILGEVVMEAGLIPGGLNIIPLPGSQIEPVME
                     DERIRKVSFTGSDEIGWELMKKFPKKKVTLELGGNAASIVDDDPPDLEFAVNRNCWGA
                     FYQAGQSCISVQRMYIHDKIFDKFMDMFIARTKALKLGDPMLEDTDVGPVIDTASADR
                     IMNWVDEAVDRGAKLLTSGHRDGCLIEPTVLTNVPPTCNVSCDEVFGPVVHIERYKEF
                     DEVLKWVNNTRYGLQAGIFTKDIKKAFEAYKALEVGGVIVNDYPSFRVENMPYGGVKD
                     SGVGREGVRYAIEEMTELKLMVVDLKDYK"
     gene            complement(4406..4657)
                     /locus_tag="VI935_09140"
     CDS             complement(4406..4657)
                     /locus_tag="VI935_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25800.1"
                     /translation="MKSPEARSKVTSFKQTAPLAGASWAWRTVCHCEHIHFIQCKLHE
                     AIPDLDCFVVSLLAMTFPCCNSNDNDIGNPAALYRELSS"
BASE COUNT         1452 a         1427 c         1003 g         1638 t
ORIGIN      
        1 gcattaatta taaaaggcat gagattcttc ccttcacgcg ctgcctcggc gatagctcca
       61 agatgctttt ttaccgcctc gttatctctt ttcctcttta tctcccgaag cctttctctt
      121 tggttgttat ccacttcttc atcaattttc agaagttgca tcggtttttc attgtcattt
      181 aggtatttgt tcaccccaac aattaccttt tcacctctat ccagctccaa ctgataccta
      241 taagctgcat cagcaatctc tttctgagga aaaccaatgt caatagcctt gataattccg
      301 ccaagctcgt caattcttct tatgtaatcg agcgcttcct tttctattcg atttgtgagt
      361 gcctcgatga catagcttcc acataacggg tcaattgaat ttgcaacccc gctttcttcc
      421 gcaataatct gctgtgtcct gagggctact gttacggact cctctgtagg aagggctagc
      481 gtttcatcca tagaatttgt gtgtagagac tgagcgccgc cgagaactgc agccaaggct
      541 tgaatagaaa cccttacaat attatttaaa ggttgctgtg ctgtaaggct gcatccagcc
      601 gtctgggcat gggttctaag catccatgat tttggattct tagccccaaa cctttccctc
      661 attatctttg cccatattct tcttgaagcg cgtaattttg caacctcctc caaaaaatcg
      721 ttatggacat taaagaagaa agaaagctga ggtgcaaaat cgtcaacatt gagtccacgc
      781 tctatgcact cctgaacata tccaatcccg tcggcaaggg taaaggcaag ctcctggatc
      841 gctgttgaac cagcctcacg aatgtgatag ccagagattg acacaggatg ccacttggga
      901 acccatttcg cgcagaattc aatcatgtca acaacgattt ttaccgaagg cctagggggg
      961 caaatccact ccctttgggc aataaattcc tttagcatat cgttctgaat tgttccatct
     1021 agcttttccc aactcacccc ttgtttctca gccaatacaa gatacatagc aagtattatg
     1081 ctagcactac agtttatagt catagaggtt gttacttcgt caagccttat tccgtcaaag
     1141 agcacctcca tatcagcaag agatgaaacc gcaacccctt cctttccaac ctctccactc
     1201 gcccttggat ggtcgcagtc ataacccatg agggtaggca tatcaaacgc agtacttaaa
     1261 cctgtctgac cctgctctag taaaaattta aatctcctgt ttgtatcctc gggagagcca
     1321 aaccctgcaa actgacgcat ggtccagagc cttccctgat acattgtgtt ataaattcca
     1381 cgtgtaaatg gataggagcc aggaaaaccg agatcacgaa gatagtcgaa atcctctagg
     1441 ctattagggg tagcaagagg aggtacatcc atacctgaaa ctgtggtaaa tttaacctcc
     1501 ctttttggga tgtttttata ttcgttctcc cagatttttg tcttttcttt gatcttttcg
     1561 agttcttttc cgttgaacga cattgttccc tcttaattct aactgtaggg gagggtcttg
     1621 cacccgccct atcctttggg caaccgcaag ggttgcccct acaaattatg aatattcttt
     1681 caatacctga tttgcaatca caattctttg aatctcagac gttccttcat aaatctctgt
     1741 tatcttcgca tctctaaaat gtctctcagc aggataatcc gtcgtgtagc catatccccc
     1801 atgaatctga atcgcctttg tagtaaccca catcgccgtc tcagacgcaa agagctttgc
     1861 catcgctgcc tgctttgaaa acttcatttt tctatccttc atccttgcag cctgccaaac
     1921 gagaagcctt gacgcttcta tcctcgttgc catgtccgct agcatgaact gaataccctg
     1981 aaaattatat atcggctgac taaacgcctc cctttccttc gaatatgaaa ccgcttcatc
     2041 gagtgcggcc tgagcaattc caacagcctg agagcctatt cctatccttc ccccatcgag
     2101 tgtctgcata gcaattttaa aacccaatcc ttcctcaccc agtctatttt ctattgggac
     2161 tcgacaacca tcaaaaataa gttgagatgt actcgatgcc cttatcccta acttgtgctc
     2221 aacctttcca acgataatac caggggtttc tttatcaact ataaatgctg tgatggcctg
     2281 atgtcttttg gatagatcag tagatgtaaa aaggattata acatccgctt ctgggccgtt
     2341 tgtcacccag ttttttgtac cgttgacaat gtaatattct ctattcttga cagcggttgt
     2401 tgtaatacta cccgcatcag aacctgcagc gggctcgctc aaagaaaagc atccgagctt
     2461 ctctcctttt gcaaggggga gaagatattt cttcttttgc tcttctgttc caaaacggta
     2521 aattgggtct gcgacaaggg aattatttac agacataact acacctgttg acgcacaggc
     2581 tttacatatt tcctccagag ctaatacata actaaatgta tccatgcctc ctcccccgta
     2641 ttcctcagga atgaaaattc ccataaaccc aagttctgcc atcttcttaa caagctcagc
     2701 ggggaatctt ccttctctat caagctctga agctagaggt ttaatctcgt tttgagcaaa
     2761 atccctagcg acatctcgca tcatctgctg ctcgtctgta agctctatta tgctcatttt
     2821 gagttcctgc gtggttcgtg actcgggttt cgtgattcgg tactagaaac caaacacacg
     2881 attcacaaac cacgatccac gattcactat ttataatcct tcaaatccac aaccataagc
     2941 ttcaactccg tcatctcttc tattgcgtat cgaacccctt ctcttccgac accagaatct
     3001 ttaacgcctc catagggcat attctctacc ctaaagcttg gataatcatt cacaatgacg
     3061 ccaccaacct ctagggcctt gtaagcttcg aacgcctttt taatgtcctt agtgaatatc
     3121 ccagcctgaa gcccatacct agtattatta acccacttaa gcacctcatc aaattcctta
     3181 tatctttcaa tatgcacaac aggaccgaat acctcgtcgc agctcacgtt acaggttggc
     3241 gggacattag ttaagactgt aggctctatt aaacaaccgt ctctatgtcc gctggtaaga
     3301 agctttgcgc ccctatccac tgcctcatca acccagttca taatcctatc tgctgaagct
     3361 gtatcaataa cgggccctac atcggtatcc tcaagcattg ggtcgccaag ctttaaagcc
     3421 ttagtccttg caataaacat atccatgaat ttatcgaaga tcttatcatg tatgtacatc
     3481 ctctggactg aaatgcaact ctgccctgcc tgataaaatg ccccccagca gtttctgtta
     3541 acagcgaatt ccaaatcagg cgggtcatca tccacaatgc ttgccgcatt acctccgagc
     3601 tcgagtgtaa ccttcttctt agggaatttc ttcataagct cccatccaat ctcgtcgctg
     3661 cctgtaaatg agacctttct tatacgctcg tcttccatca cgggttcaat ctgagagcct
     3721 ggaagtggaa taatattaag cccgccagga attaaaccag cctccatcac gacctctcca
     3781 agtataagag ctgaaagggg ggtctgtgat gcaggtttaa gaatcatcac gttccctgaa
     3841 gcaatcgctg gagcaacctt gtgtgcaact aggttaagcg gaaaattaaa tggggagatt
     3901 cccattacta tgcctaatgg gaatcttttc actattccaa acctttcctc gtttcctggc
     3961 acaatatcaa ttggaataag ctctcctccg gcaaatttat ttgcttcttc tgcggcaacc
     4021 ttaaatgttg tcatcgcacg acttacttcc acccttgcgg tctttatcgg ttttccaccc
     4081 tcttctgcca gggctttagc aaactcctct tttctttttt caattccgga tacaattctc
     4141 atgagtatat cgcttctttc gtaactggga gagttcctga atttcccaaa ggccttctca
     4201 gcagcgtcaa ttgccccctt tgcctcttct aatgaaggta tatttattac accaacgact
     4261 tcattattat aagggcttct tacttccctc tttttggcat ttttcaacca ctttccatta
     4321 actaaaaatc catattcctt agccatttga ttctaccccc ttttattttt attcctaaaa
     4381 accagacaaa ataattcctt cacatttaac ttgatagttc tctgtagagt gctgcaggat
     4441 ttcctatatc attatcattg ctattacaac acggaaatgt cattgcgagg agtgaaacga
     4501 cgaagcaatc taaatccggg attgcttcat ggagtttaca ctgaatgaag tgaatgtgtt
     4561 cgcaatgaca aacagtacgc catgcccagc tcgcaccagc caaaggagct gtctgtttaa
     4621 acgatgttac cttggacctt gcttcagggc tcttcattaa gattttataa aatcttatcg
     4681 caaaaaacac ctacaaatta ttaaacaaaa gattagtggt taagtcaagt tattgggaaa
     4741 tttgatgatg aagttatatt aattgccagt taagtagggg caggtcttgc acctgcccta
     4801 attttgggca ggtcttgcac ctgccctaat tttgggcaac cgcaagggtt gcccctacaa
     4861 agttgatcac acgtcctgga tatatgggtc tggtgggcca ctccttttta ttgaatttga
     4921 tcgacgatag acaagacatt ccttcgactt cgctcgggac aggcttgctc gttggatggt
     4981 acgcccacca gcaaaactgg caggctaccc atttttgatg accccatcag tattgcttga
     5041 cgattagacc taaattgaga gtcaacgtca atgcgggact ggaaagtccc gcctatccct
     5101 atttattaaa tttcgacccc ctgtcaaacc aatccgttag acggggttag aaaaccccgc
     5161 ctatcagtac taaaccgaaa gaatgataga cgagactttc tagtctcgtc aacgtcaatg
     5221 cgggactgga aagtcccgcc tatccctttt cccttccgca tctacgggcg gggttggaaa
     5281 accccgccta tcgaggatgg aggttgaaca taccgaagct gaacattcct gtcctacatt
     5341 gttataagaa tggctaaacc aaaaatacaa tacaaaacgg ggttggagaa ccgcacctat
     5401 cagacctaaa ttgagagaac aatagacgag actttctagt ctcgtcaacg tcaatgcggg
     5461 actggaaagt cccgcctatc cctatttatt aaatttcgac ccccggcaaa caaatcccta
//
LOCUS       DATDRL010000275         8778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2728054, whole genome shotgun sequence.
ACCESSION   DATDRL010000275 DATDRL010000000
VERSION     DATDRL010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8778
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2728054"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VI935_09145"
     CDS             complement(<1..710)
                     /locus_tag="VI935_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HLE25801.1"
                     /translation="MSRIFNPDFINNLITWLTTSGVRVVVVVMGAYVLLKLFSILIGR
                     VEKMIVKKDKGEFFATLETEKRVKTLGSILRKGTFVALFIIALMMVLKEVGMDIVPII
                     TGAGIVGLAVGFGAQNLVRDIISGFFIIMENQVRVGDVAVINGTGGLVEEINLRTIVL
                     RDLEGTVHVFPNGTINTLSNKTRGWSRYVIDVGVAYKENVDKVIEVLTDIGKELFKDE
                     KFRPMILEPLEVLGVDDFG"
     gene            936..2768
                     /gene="mutL"
                     /locus_tag="VI935_09150"
     CDS             936..2768
                     /gene="mutL"
                     /locus_tag="VI935_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HLE25802.1"
                     /translation="MGKIRILHDRIVSKIAAGEIVERPVSVVKELIENSLDAGSTEIK
                     VELKTGGKKLILISDNGEGMTRDDALLSLERHATSKIRKAEDLFSIKTLGFRGEALPS
                     IAGVSRFRLITKAKDEIVGTKITVEGGTIKRVEEIGCPNGTTVEINDLFYNTQPRLKF
                     MKTTETELSNILDIIQRESLSHPNVGFEVIHEGRILLQLPHRKNIEERFPEIFPNTDL
                     FEIEAKAEEIKIYGFMSKPENERTTTQKLYTYVNKRTVRDKFLTRMVIDSYGRLIERG
                     KFPQGVLLIEIPFEEVDVNVHPTKNEVRFRRARLVGDLIKAGISDMLRNAPWIKDYHS
                     RVENSVHGFFERRGIFEVTQNQHKGAFLKRETPEINENDSSLKITNNQVQAKPNEIAT
                     SHKPIIKDISSPEALKPEKLFKEEGFFSNLEVIGQLGELYIICASGRGMILIDQHAAH
                     ERINYEKLKDAYLIRELQTQELLFPLLIELSPDEVELLGQYSEEIASLGIKLEEFGRS
                     SFLIRSIPALLKGADLEKLLKGIIGELSMLGKEKGLSERMDHIISTMACHSSIRAGKE
                     LNHEKMKAILRDLDCARFPHSCPHGRPVARELTYEELEKMFKRS"
     gene            2771..3343
                     /locus_tag="VI935_09155"
     CDS             2771..3343
                     /locus_tag="VI935_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HLE25803.1"
                     /translation="MKEFIISRLRESADLKLLFAKQATKDVIKAVNIIEKSLKSSGKI
                     LIFGNGGSAADAQHIAAEFVNRYLKERNALPAIALTTDTSILTSIGNDGSFENIFSRQ
                     IEALGKKGDVALGLSTSGRSRNVVRALRVAKSFGLKTIGFTGGDGGEIKKIVDCNINV
                     HSVSTPRIQELHITIGHIICELIEDIFSNK"
     gene            3355..4017
                     /locus_tag="VI935_09160"
     CDS             3355..4017
                     /locus_tag="VI935_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HLE25804.1"
                     /translation="MKYYPVNLDLKGKKCLVVGGGRVAERRVISLLECEADVIVISSR
                     VTPKLERLSVNKKIAYKSRSYKPSDLREVYLVIASTNNQKLNRHIGERARKLGALVNI
                     VSEPKLSDFTVPSVLRRGNLLLAISTSGTSPALSKRLRSNLETILGKEYEIFTNIMGA
                     IRTKCRSLSVNKRRRIYSQVAMSEIPELLREKRYKKAEKVLMNITGLSFNQIKLSSKP
                     QS"
     gene            4057..4254
                     /locus_tag="VI935_09165"
     CDS             4057..4254
                     /locus_tag="VI935_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HLE25805.1"
                     /translation="MQVRCPRCVSFVEWEGNPWRPFCSERCKLVDLVAWVDEEYKIPE
                     QRSSEEDQMQGDAVRLGNEDN"
     gene            4286..4672
                     /locus_tag="VI935_09170"
     CDS             4286..4672
                     /locus_tag="VI935_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HLE25806.1"
                     /translation="MTIGVYQSVSRLDPTEEKTEIIEEFMREEGKHTMMAVKRLDELG
                     LVTPKKSGEIAERFGETIGRAAGFFGWNGVCFFMQIGEEVERLFLEAASRICKNKNDR
                     EILNLIIFDERKHGDWWKRKSYHRRH"
     gene            4676..5248
                     /gene="efp"
                     /locus_tag="VI935_09175"
     CDS             4676..5248
                     /gene="efp"
                     /locus_tag="VI935_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HLE25807.1"
                     /translation="MAVADTGRFYKGLKIEFEGEIWEVLEYHHSKIAQRSPVVKTKLR
                     NIITGSVQERNFRSGETFNLPDVERKKMQFHYKDEIGYHFMDSETFEEYAFGENHLGD
                     TVRFLKEQEEVNILFYKGKTMGVELPITVDLEVIDTEPGFRGDTATGGSKPAKLETGV
                     IISVPLFIDIGDLIKVDTRSGTYIERIKKK"
     gene            5401..5727
                     /locus_tag="VI935_09180"
     CDS             5401..5727
                     /locus_tag="VI935_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HLE25808.1"
                     /translation="MTEETKRRKNPTLALLLSAIFPGLGQIYNNQVLKGIALIALNTV
                     VNFLLINPLEGLISSLDSKPDNSTLFIVAAYTIIGLILWVYAIIDAKKTAERINQRVP
                     KIMQNG"
     gene            complement(6175..6429)
                     /locus_tag="VI935_09185"
     CDS             complement(6175..6429)
                     /locus_tag="VI935_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25809.1"
                     /translation="MAGIVGADIAAKPWASVPMATKKLMQIMYYNFKNAGAQEEPVES
                     LHEFHTDIYPWQIDFMTSNEDMDSKDQVVICSCGESIDSL"
     gene            complement(6926..7444)
                     /locus_tag="VI935_09190"
     CDS             complement(6926..7444)
                     /locus_tag="VI935_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE25810.1"
                     /translation="MRLNLFLTLAIFTMFFGCAATEQHVNELYEKQAKLEADTERLSQ
                     DILLLKSQINNLKQSNSKLKKRLDGLEKKGAFSGSPPAKSPEALYRVAESYYREGKFE
                     EAILEFQRFIDTYPQDKRAPNSYLKQGLSLIKIGRKDEARFFLETLVDKFPESEEAKI
                     AKEELKEITGKN"
     gene            complement(7661..8137)
                     /locus_tag="VI935_09195"
     CDS             complement(7661..8137)
                     /locus_tag="VI935_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25811.1"
                     /translation="MRKLVAVVLIITSLAVSARAQESNPSFGLGIGFTVDPETFLMGG
                     RFDVPLAYGFSLGPFVQIGVSEDNTLVGISGNVKYTIPVPKAPKVLPSLEAGAGVLIS
                     DQHDDTESSFLIVLGGGLDYMITRNIGLGGHVYTNPAPGPGEDFFVSFLFGLNVRL"
     gene            complement(8318..8590)
                     /locus_tag="VI935_09200"
     CDS             complement(8318..8590)
                     /locus_tag="VI935_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLE25812.1"
                     /translation="MPDNKLPLPDSFVAKVMIPQIIREALLTLTPREKKIIKMRFGID
                     HDATYTLDEIGRRFNVTRERIRQIEKRALEKLETSRIGEVLKDFLD"
BASE COUNT         2941 a         1623 c         1682 g         2532 t
ORIGIN      
        1 ccgaagtcat caactcctag cacctcaagg ggctctaata tcattgggcg gaatttttca
       61 tctttaaata gttcctttcc tatatctgta agcacctcaa taactttatc cacattctct
      121 ttatacgcca caccaacgtc aattacatag cgtgaccaac ctctggtttt attcgatagt
      181 gtatttattg taccatttgg aaagacatgt acagttccct caaggtccct taacacaatt
      241 gttctaagat taatttcctc tacaagtcct cctgtcccgt taatcacggc aacatctcca
      301 acacgaacct gattctccat aattatgaaa aaaccgctta taatatccct tactaaattc
      361 tgtgcaccga atccaacagc gagtcctacg ataccagcac cagttataat tggtacaata
      421 tccattccaa cttctttaag aaccatcata agagctatta taaaaagagc tacaaaagtc
      481 cctttcctca atatgcttcc taatgtcttc accctttttt cagtttccag tgtggcaaag
      541 aactcccctt tgtctttttt cacaatcatt ttttctaccc ttccaataag aatggaaaac
      601 aactttagaa gcacataagc ccccatgact acaacaacta cacgaacacc agaagtcgta
      661 agccacgtaa taaggttatt aataaaatct ggattaaata ttcttgacat gggctacctc
      721 ctttaaaaaa tatctatata taaatataga acctatttat gatttagcta tctctagaat
      781 tttgtgattg aaataattcc gaaagacaag attaaagttt gaagagtact caagatattg
      841 gataaaaaat taatattgac gggtctaatt ttactcagtc aaaaacacct gtttttgact
      901 gaacataccc ttttatgtaa aatggcattt tagaaatggg aaagattagg attcttcatg
      961 ataggattgt ctccaagatt gcagcaggag aaatagtaga aagacccgtc tctgttgtaa
     1021 aagagctcat cgagaactca ctagacgctg gaagtacaga gataaaagta gagcttaaaa
     1081 ctgggggaaa gaaactcatt ttgatctctg ataacggcga agggatgaca agggatgacg
     1141 cacttctatc tctcgaacga catgccacaa gtaaaatcag gaaagccgaa gatctatttt
     1201 ctataaagac acttggcttt cgtggagaag cgctgccgag cattgcaggg gtttccagat
     1261 ttaggctcat aacaaaagca aaggatgaaa tcgttgggac caagatcaca gttgaaggcg
     1321 gaactataaa aagagtggag gagataggat gccctaatgg aacgacagtt gaaataaatg
     1381 atcttttcta caatacacag ccgaggctaa agtttatgaa aactaccgaa actgaattat
     1441 caaatatcct tgatattatt caaagagaat cgctttctca cccaaatgta ggatttgaag
     1501 tcatacacga aggaagaatc ttacttcaac tcccccatag aaaaaatatt gaagaaagat
     1561 tcccagaaat cttcccgaat accgaccttt ttgaaatcga agcaaaagct gaggaaatta
     1621 agatttacgg ttttatgagt aagcccgaga atgagaggac tactacacaa aaactataca
     1681 catacgtaaa caaacgcact gtaagagata agtttctcac tcgaatggtg attgactcct
     1741 atggaaggtt aatagaacga ggaaaatttc cgcagggagt tcttctaata gaaatcccgt
     1801 ttgaggaagt tgatgtaaac gttcatccta caaagaatga ggtcaggttt agaagagcaa
     1861 gactcgtagg tgatttaatc aaagctggga tttcagacat gctaagaaat gctccttgga
     1921 taaaagacta ccattcaagg gttgagaatt cggtacatgg attttttgaa agaaggggaa
     1981 tttttgaggt tactcaaaat caacacaaag gagctttcct gaagagagaa actcctgaaa
     2041 ttaacgaaaa cgattcttca ctgaaaatta cgaataacca ggtacaagca aaaccaaatg
     2101 agatagcaac atcccataaa ccaattatta aagacatttc aagccccgaa gctcttaagc
     2161 cagaaaagct ttttaaagaa gagggtttct tctctaactt agaagtaata ggacagttag
     2221 gagaactcta cataatctgc gcctccggaa gaggtatgat tcttattgat caacatgcgg
     2281 cgcacgaaag aataaactac gagaagctta aggatgccta tttaatcaga gaactccaga
     2341 ctcaagaact actttttcct ttattaattg aactctcccc agacgaggtt gaactcttag
     2401 gacagtatag cgaagaaatt gcgtctcttg ggataaagtt ggaagagttt ggaaggagct
     2461 cctttcttat aagatctatt cccgcactac ttaaaggtgc cgatcttgaa aaactattaa
     2521 aaggtataat tggagagctt tctatgctag gaaaagaaaa gggtttaagt gagcgaatgg
     2581 atcatattat ttcaactatg gcatgtcatt cttctattcg cgctggaaaa gaattgaatc
     2641 atgaaaagat gaaagccatt ttaagagacc tggattgcgc caggtttcca cactcttgtc
     2701 cacatggaag gcctgtggca agggaattaa cctatgagga actcgaaaag atgtttaaga
     2761 ggtcctgaat atgaaggaat ttatcatatc aaggctccga gaaagtgccg accttaaact
     2821 actctttgca aaacaagcaa caaaagatgt tataaaggct gtaaatatta ttgagaaatc
     2881 acttaaatca agtggaaaaa tattgatttt tggaaacggt ggaagtgccg ctgatgctca
     2941 gcatattgca gcagagtttg taaaccgata tctaaaggag cgtaatgctc tcccagccat
     3001 agcacttacc acggacacat caattctcac ctcaattgga aacgatggct cgttcgagaa
     3061 tatattctca aggcagattg aagcacttgg aaaaaaggga gatgttgcat tgggattgtc
     3121 aacaagcggc agatctagaa acgttgtaag ggctcttaga gttgcaaaat cattcggtct
     3181 taaaaccata ggttttacag gtggagatgg aggagaaata aagaagatag ttgactgtaa
     3241 tataaacgtg cactccgtgt caacgccgag gattcaggag ctacatatca ctatcggtca
     3301 tatcatatgt gaactcattg aagatatttt ctccaataaa tgaaatgatt gaatatgaag
     3361 tattaccccg ttaacctgga tttaaagggc aagaagtgct tagttgtggg aggtggaagg
     3421 gttgcggaaa gaagggttat atcactcctg gaatgtgaag cagatgtgat agttataagc
     3481 tctcgtgtta ctccaaagct agagaggctt tcagttaata aaaaaatcgc ctataaatca
     3541 aggagctaca aaccgagtga tctaagagaa gtatatctcg tcatcgcatc aactaataac
     3601 caaaaattaa atcgtcacat tggagaaaga gcgagaaaac tcggagcttt agttaatata
     3661 gttagcgaac ctaaactgtc tgatttcaca gtacccagcg tattgaggcg tggtaacctt
     3721 ttgctagcaa tctccacaag tggtactagt cctgcactgt caaaaagact taggtcgaat
     3781 ctcgaaacaa ttctgggaaa agaatacgaa atatttacta atattatggg agctataaga
     3841 acaaaatgtc gaagcctctc agtcaacaag agaagacgca tatactctca ggttgcaatg
     3901 tccgagatac cagagttatt aagagagaaa agatacaaaa aagcagaaaa ggtacttatg
     3961 aacattactg ggttaagttt taaccaaatt aaactttcat cgaagcccca atcctaactt
     4021 gatttatcac actacctcag ccataatttc aaattaatgc aggttagatg cccaagatgc
     4081 gtcagttttg ttgaatggga ggggaacccc tggagaccgt tttgttccga aaggtgcaaa
     4141 ctcgtagacc ttgttgcttg ggtcgatgag gaatataaaa ttcctgaaca aagatcatca
     4201 gaagaggatc aaatgcaggg ggacgcagtc cgattaggaa atgaagacaa ctaacttact
     4261 gaaactagtt tacatttcca agagaatgac tattggagtt tatcaatctg tttcaaggct
     4321 tgatccaacg gaagaaaaaa cagaaataat tgaggagttt atgagggaag agggaaagca
     4381 tacaatgatg gctgtaaaga gactcgatga gctcggactt gtaaccccta aaaaaagcgg
     4441 tgaaatagca gaaagattcg gtgaaactat aggtagagca gcaggatttt tcgggtggaa
     4501 tggagtttgt ttttttatgc agataggtga agaagtagaa aggttattcc ttgaagccgc
     4561 ctcgaggatt tgtaaaaata aaaacgacag agaaatcttg aatcttatta tattcgatga
     4621 aagaaaacac ggtgattggt ggaagaggaa atcataccat aggaggcatt aataaatggc
     4681 agttgccgat actggccgct tttacaaggg gctaaaaatc gagttcgaag gggagatctg
     4741 ggaggttcta gaataccatc actcaaagat tgctcaaaga agccccgttg taaaaactaa
     4801 gctaagaaat attatcactg ggtctgttca agaaagaaac tttcgatctg gagagacatt
     4861 caacctaccc gatgttgaga ggaagaagat gcagtttcat tacaaggatg aaattggata
     4921 ccactttatg gactccgaaa cgtttgagga atacgcgttt ggcgagaacc atcttggaga
     4981 tacagtaagg tttctaaagg aacaggaaga ggtgaatatc cttttctata aaggcaaaac
     5041 gatgggagta gagctaccga taacggttga ccttgaagta attgacaccg agcccggttt
     5101 tagaggtgat actgcaacag gcgggagtaa acctgcaaaa ctcgaaacag gtgtaatcat
     5161 ttcggtaccc ttgtttattg acattggaga tctaataaag gttgatacta gaagtggaac
     5221 atatatagaa agaattaaga aaaaataata ataaattaag tttttttaat cagtctttct
     5281 atagttcaat taggtttcta atttgaaatt cttatatatc gtcacctaga tatagactta
     5341 catcatgtca atcctattac ctaattcaaa atcttcgcaa ctcaagtata gggtccatat
     5401 atgacggaag agacaaaacg aagaaaaaac cctacccttg ccctcttact ctcggcgata
     5461 ttccccgggc ttggacaaat ctacaataac caagtactta agggaatagc tctcatagcg
     5521 ttaaatacgg ttgttaattt tctattgatt aacccacttg aagggttaat ttcgtcctta
     5581 gattcaaaac cggataactc gacccttttt atcgtagctg cctatacaat aatagggttg
     5641 atcctatggg tttacgcaat catagatgct aaaaagacag cagaaagaat aaatcaaaga
     5701 gttccaaaaa taatgcagaa tggttaatta tttaacctga aagctccttt aacttttctc
     5761 tcgcgatctt agcctcttca gactttggaa acttatatat aagagtctca agaaaaaact
     5821 ttgcttcatc ttttcttcca attttaataa gaggaagtcc ttgttttaaa tatgaggtcg
     5881 gagccctttt gttttgcggg taagtatcaa taaatctcta aaattcgaga atcgcatcag
     5941 cttatttacc ctcttgataa tagaactccg cttttaaatg caaagcgtca ggagattcaa
     6001 ctaattgttc atgaaaaatt gtaaatggat ttgcagaact tatcacccaa aaaaatctat
     6061 ttatatgaag ggaggtggcc gaatggaggg atggagtaag tatggagcat tcttagtcac
     6121 ctcccttctt gcggttttta gttcaattta taaaaaattc aagataaatt aatgctataa
     6181 agaatctatg gactcaccac aactgcatat aacgacctga tctttggagt ccatatcctc
     6241 gttgcttgtc ataaaatcta tctgccaagg atatatatcg gtatgaaact cgtgcaaact
     6301 ttctactggt tcttcttggg ctccagcatt cttaaaattg taatacataa tttgcatcaa
     6361 ctttttcgta gccattggta cgctagccca aggtttagcg gctatatccg ctcctactat
     6421 accagccata acattaaccc tttgtgtctt aggaaggcta tcccaccaat cctgaaggtt
     6481 cttgttaagc tcagatatct taggaatact gctgtttttg attgaaaata cctccagtga
     6541 tttaggtatg caatgatcat gccagagggg ttaatatttt taagaataaa ccttataaag
     6601 gttttaaata gggaaagtgg tttctgaaaa taaaaaaggg aatgaatgtg taatattaca
     6661 ctgtgtaagg tgtgtaaatt tacacaaaaa ttgggagacg gcatgttgat agctattata
     6721 ttgcttgatt aggaaggcaa ggtatgagcg cataatctta ttttcttttc attcttactc
     6781 aagctgtata atatttgcca tatctcgaac atgcgctatt caaaatatga ggatttataa
     6841 gaaaagtaga cgggattctt tgtggactaa ggttttaaca actcttatgc cttggtatat
     6901 tggattaaca acaacatagg ttatatcaat tttttcctgt aatctctttc aactcttcct
     6961 ttgctatttt agcctcttca gattctggga acttatctac gagggtctct agaaaaaacc
     7021 ttgcttcatc ttttcttcca attttaataa gagaaagtcc ttgctttaaa tatgaattcg
     7081 gagccctttt atcttgtggg taagtatcaa taaatctctg aaattcgaga atcgcttcct
     7141 caaattttcc ttctctataa taagattctg ctacccggta taaagcctca ggggatttag
     7201 ctgggggaga acctgagaat gctccttttt tctctagccc atccaatctt ttttttagct
     7261 ttgaattaga ctgtttcaga ttatttattt gacttttaag tagtaaaatg tcttgagata
     7321 accgctcagt atcagcctca agcttagctt gcttttcata caactcattt acatgctgtt
     7381 cggtagcagc acatccaaaa aacatagtaa aaatagccaa ggtcaaaaaa aggttgagtc
     7441 tcatcagagg attttatata aggattatgc atatcataaa aaagacttaa tatcaacaaa
     7501 tattatccat aaattccttc aaataagtca attaactcta tctgaggttc tcgattcagt
     7561 agatttccag acacacctat cagattaggc aaattttgcc ttataaatac tcttaagcag
     7621 gattcgaagg ttggaaaagg ttgaagttta tataagttaa ctaaagtcta acattcagtc
     7681 caaagagaaa tgaaacaaaa aaatcttcac ctggaccggg agctggattt gtatacacat
     7741 gccctccgag tccaatattt cttgtaatca tataatctaa ccctccccct aaaacaatta
     7801 aaaaagatga ttcagtatcg tcatgttggt cggaaatcag aacacctgca ccggcttcaa
     7861 gcgaaggcag aactttaggc gccttcggca ccggtatagt gtatttaaca tttcctgaaa
     7921 taccaaccaa agtattatcc tcactcacac caatctgaac aaatggtcct aatgagaaac
     7981 catacgctag aggaacatcg aagcgtccgc ccatcaagaa agtttcaggg tctacagtaa
     8041 atccgattcc gagcccaaaa cttggattac tttcttgtgc cctagctgaa accgctagcg
     8101 atgtgataat gagaactacg gcaaccagct ttctcatttc tttccccttt aagtatattg
     8161 atttcttata tactaaggtc aaagaactct aaaaactcga ggcggcattt atgttgagct
     8221 cacggctaaa gaacaaaatg gtactgagaa caaccgttgc tgttataaac atgaaaattc
     8281 tttccatctt atgttgcctc cttatatttt ttgtttctta atcaagaaaa tcctttaaga
     8341 cttcaccgat ccttgatgtt tcaagctttt caagcgctct tttttcgatt tgccttatac
     8401 gctcacgcgt gacattaaac cttcttccaa tctcgtcaag cgtatatgtg gcatcatgat
     8461 ctataccgaa cctcatctta attatctttt tttccctagg agtaagcgtt aagagagctt
     8521 ctcttattat ttggggaatc ataacttttg ccacaaacga atcgggtaaa ggtagtttgt
     8581 tatcgggcat aaaatcaaga aaagttgtct tctcatcatc gattataggt gagtctaatg
     8641 tcaatacaaa gtgaatatgt cacccctgat gcaaagtgaa aatgtcacct gtacgtagat
     8701 tctcaagggg tagagtatga gttttaatct ttttagtttc accagaacta atctccttaa
     8761 aattcagata ttgatctt
//
LOCUS       DATDRL010000276         4138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_89640, whole genome shotgun sequence.
ACCESSION   DATDRL010000276 DATDRL010000000
VERSION     DATDRL010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89640"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VI935_09205"
     CDS             <1..413
                     /locus_tag="VI935_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25813.1"
                     /translation="VRENVTDIRQAQKVLSEELYRYNYRQVHSTTGEVPYFRFQRALE
                     EKRSLFREFKIKPPYGSVKDIFCLRVNRTIDAYRKISINSLELKVNGAVPRETVNLRI
                     YPMSNGISEVRFWCNDKLIDVKRVKNSDLQIVHF"
     gene            628..1092
                     /locus_tag="VI935_09210"
     CDS             628..1092
                     /locus_tag="VI935_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLE25814.1"
                     /translation="MRGLKTILSKHPLFKGLDKRYLQLIVDCASDVHFNSGDLIFHEG
                     EEANQFYIIREGRVALEVVLVPGREPIIIQVIGEGDVLGWSWLFPPYRWHFDTRAVAP
                     TQAIALDGKFLRTKCEKDHHLGYELMKRFANIIEKRLRAMRLQNPDMYAIHA"
     gene            1440..1580
                     /locus_tag="VI935_09215"
     CDS             1440..1580
                     /locus_tag="VI935_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25815.1"
                     /translation="MNEPKVKGCFVGIDVSKNALDVFVRPSGECWTQSNGDYSQLCEK
                     LK"
     gene            1599..2396
                     /locus_tag="VI935_09220"
     CDS             1599..2396
                     /locus_tag="VI935_09220"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HLE25816.1"
                     /translation="MLEPTGGYEIGLLNALIKEGLRVSRQHAYRIHHHAKGSGQMAKT
                     DRLDASMIAHYAECYSEKIEPMTVFLEDQALLQQLSARRRQLVDMRAAEKNRLGLPGL
                     SKRLRISYEKIIRVLSEEIEEIEEQIQGIIEGDEQWRKKQALLETVVGVGTRVSRVLL
                     ANLPELGRVNRKRIAALVGVAPYQHESGKFKGQQRIKGGRSEVRSVLYMAVLSAKEHN
                     PEIRAFYERLLEKGKKKKVALVACIHKLLRILNAILSKWACYQSSTG"
     gene            complement(2635..3168)
                     /locus_tag="VI935_09225"
     CDS             complement(2635..3168)
                     /locus_tag="VI935_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HLE25817.1"
                     /translation="MGLTKKNILITGFPGVGKTTLIKKLTENPIDLKPVGFYTEEIRE
                     KGIRKGFKLISLDGGEGILSHVDVKSPYTVGKYKVDIDGFENFIDYIPFFDPTSSFII
                     IDEIGKMECLSEKFKKLLKEILDSEKVLVATIALKGGGFIEEIKKRDDIKLFEMKQDN
                     RESLLFEILRNIKTLIP"
     gene            3376..>4138
                     /locus_tag="VI935_09230"
     CDS             3376..>4138
                     /locus_tag="VI935_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014460100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLE25818.1"
                     /translation="MNIKQLFDLTGQVAVVTGGYTGIGRQMAEGLAEAGANLVICARN
                     LDGCIKAAEEIRKRTGVETIALKCDVASAEDVEKMVRETFNKLGKIDILVNNAGIATG
                     GLPEDMKLEDWENVLRINLTGTFLCSKEVGKVMIKSKKGKIINLASIMGFQTTELVSA
                     PSYVTSKGAIITLTRELAVRWIHHNINVNAIAPGWFPTHMTDPVLSPDKMGKGEDLLR
                     SIPARRFGGEDDLKGATVLLASSASDYIVGQVIVVD"
BASE COUNT         1287 a          735 c          950 g         1166 t
ORIGIN      
        1 gtgtcagaga aaacgttaca gatataagac aggcacaaaa ggttctcagt gaggagctct
       61 atagatataa ctaccgccag gttcattcaa caactgggga ggttccttat tttaggtttc
      121 agagagcgtt agaagagaaa aggtccttat ttagagaatt caagataaag cccccttatg
      181 ggtctgttaa ggacatattc tgtctaaggg tcaataggac tatagacgct tacagaaaaa
      241 tctcaatcaa tagcctagag cttaaagtta acggggccgt cccaagagag actgtaaatc
      301 ttcgtattta tcctatgagt aatggaatat ctgaggtaag attctggtgc aatgataaac
      361 ttatagatgt caaaagggtt aaaaatagcg atttacaaat tgtgcacttt taattcttaa
      421 actgtgcact ttaaatttta gctaacacac ccgatctttt acccactaat tttttttgac
      481 tattgttaat gcgataatca ttatataact tatccagaaa ttaggataat attataatta
      541 tcttttaata agttgagata gagccttcgt cgagagtcga aacccatagc aatgctattg
      601 ctgctgtctc aacaaaggag agaatatatg agaggactta aaactatact ttcaaaacat
      661 ccacttttta agggacttga taaacgctac cttcaactga tagtagattg tgcatccgac
      721 gtgcacttca actcaggaga tcttatcttc cacgagggag aggaagctaa tcagttttat
      781 ataattcgtg agggcagagt ggcgcttgaa gtagtgcttg tgcctggacg tgagcctatt
      841 atcatacagg tgattggaga aggagatgtg cttggttggt cgtggctttt tccgccatac
      901 aggtggcatt ttgacacccg ggctgtcgcc cctactcaag ccattgctct tgatgggaaa
      961 ttccttcgta caaaatgcga gaaagatcac caccttggct atgagcttat gaagcgattt
     1021 gctaacatta tcgagaagcg gcttcgggca atgagattac aaaatccgga tatgtatgct
     1081 atacatgcct gaaaggtagt tgaacgcctt tatccttact gattagttct gtagtaatac
     1141 ttgtactaga caattcacta tagcttacta aatggtctcc gctgtcatcc atgataacat
     1201 agaattgtca tttgtatgtt aaatgactag gttgagttat cctgatcaga taaccaaccg
     1261 gggcaaagca gactgtttgt catttggtcc taagagaccg tctcgcagca aagtcgcttt
     1321 gcccttcttt tcacagagtc cgaacagttg taaaggccat aatacatgag cctgaattta
     1381 caagtaagga tgtgaaaagc aaacagttgg ttaattgaac ttatatagga ggataaccga
     1441 tgaacgagcc aaaagtaaag ggatgttttg tagggattga tgtttccaag aacgcgcttg
     1501 atgtctttgt acgaccaagt ggagagtgtt ggacacaatc aaacggagat tattctcagt
     1561 tatgtgagaa actcaaatga ttgaatccga ctctgattgt gctagagcca acaggcggat
     1621 atgagattgg tttattgaat gcgttaatca aagagggcct ccgtgtaagc cgtcagcatg
     1681 cttatcgtat tcaccatcac gccaagggta gtggtcagat ggcaaagact gataggctgg
     1741 atgcgtcaat gatagcacac tacgccgagt gctatagtga gaagatagaa ccaatgacag
     1801 tatttttgga ggatcaggca ctgttgcagc agctaagtgc aaggaggcgt caattggtcg
     1861 atatgcgtgc tgctgagaag aatcgacttg ggttaccggg tctttcaaaa agactaagaa
     1921 tcagttatga aaagattatt agagtattaa gtgaggagat agaagagata gaggagcaga
     1981 tccaggggat catcgagggc gatgagcagt ggaggaagaa acaggctctt ttggaaacag
     2041 ttgtaggagt tgggacaagg gtatcaaggg tattgttagc gaatttgcca gagcttggaa
     2101 gggtcaatcg caagcgaatt gcagcgttag ttggggtggc accttaccaa catgaaagtg
     2161 gtaaattcaa ggggcagcaa cgtatcaagg gaggacgttc agaggtaagg tcagttttat
     2221 acatggcagt gttatctgct aaagaacata acccagagat cagggcattt tatgaaaggc
     2281 tattggagaa gggcaaaaag aaaaaggttg cactggtagc gtgtatacat aagttactac
     2341 gaatactaaa tgcaatactg tcaaagtggg catgctatca gtcatcaacg ggttgagagg
     2401 tattgagaga tcatatgggg ggaatatttg ttaaaacagg ttgactttta acacagttgc
     2461 tgcgaggaac ccttcgacat agctcaggat gcgggacaag cctgtcctga gtttaacgaa
     2521 ggaaatgtcc cgcctatccg ctgcaggctc cgcgaatgtg ttagcaatga caatacagca
     2581 ttgtggaaac cctatagctg cagggtattt cactctgcat tagaaggggt aaatttaagg
     2641 tattaatgtt tttatatttc ttaaaatttc aaataataga gattccctat tatcttgctt
     2701 catctcaaat agctttatat catccctttt ctttatctct tcgatgaatc cgccaccctt
     2761 tagggctatt gttgctacta gcactttctc agaatctaaa atctctttta agagtttttt
     2821 aaatttttca gataaacact ccatctttcc aatctcatca ataataataa aactgcttgt
     2881 agggtcaaag aatggtatgt agtcaataaa attttcaaag ccatctatat caaccttgta
     2941 tttaccaact gtgtaaggac tttttacatc gacatgagat agaatccctt cgcctccatc
     3001 aaggcttatt agcttaaatc cctttctaat tcctttctct cttatctcct ctgtataaaa
     3061 accaactggt tttaaatcta ttggattctc agtgagtttt ttaataagag tcgtcttacc
     3121 aacacccggg aatccagtta taaggatatt cttttttgtt aagcccattg attgtatcgt
     3181 tccggtatag ctttcgagag ttactcctgt atttaagtct aagaaaacag catagctgaa
     3241 aaaagggaac tccatcaaga aaaatccagt tctcttaaaa tcatagcggg gtctgagatt
     3301 tgattgtatt ttctttggaa gtgtttgaaa gaaaggttaa gataaataaa caaagccagc
     3361 aagagaggac cttttatgaa tataaaacaa ctatttgatc ttacagggca agtagccgta
     3421 gttacaggag gttatacggg tattggaagg cagatggcag aggggcttgc cgaagctggc
     3481 gcaaatctgg ttatatgcgc aaggaatttg gatggatgta taaaagcggc agaggaaatc
     3541 agaaaacgga cgggagttga aaccattgca ttaaagtgcg atgttgcaag tgccgaagat
     3601 gttgaaaaaa tggtgagaga gactttcaat aaactcggta aaattgacat actagttaat
     3661 aacgccggca tagctacagg cggactaccg gaggatatga agctggaaga ctgggagaat
     3721 gtccttagaa taaacctgac gggcactttt ctctgctcta aagaagtagg aaaggttatg
     3781 attaaatcaa agaagggtaa gattattaat ctggcatcta taatgggatt ccaaaccaca
     3841 gagttagtaa gtgccccgtc ttatgtgaca agtaagggag ctatcatcac tcttacccgt
     3901 gaactcgccg taaggtggat tcatcacaat ataaacgtaa acgccatagc ccccggatgg
     3961 ttcccaacac acatgacaga ccctgttctt agcccagata agatgggaaa gggagaggat
     4021 ttattacgct ctatacctgc aaggagattc ggtggagagg atgatctaaa aggagctaca
     4081 gttcttcttg cttcctcagc ctcagattac attgttggac aggtaatagt cgtagatg
//
LOCUS       DATDRL010000277         4479 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2958552, whole genome shotgun sequence.
ACCESSION   DATDRL010000277 DATDRL010000000
VERSION     DATDRL010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2958552"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..216
                     /locus_tag="VI935_09235"
     CDS             52..216
                     /locus_tag="VI935_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25819.1"
                     /translation="MKIKKIILASFLSLALLASYGCAFLVGAAAGGAGGYILRDQGYE
                     VQSPVEKEGG"
     gene            221..628
                     /locus_tag="VI935_09240"
     CDS             221..628
                     /locus_tag="VI935_09240"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HLE25820.1"
                     /translation="MGKKIFMVWISVIVFFMIFSNARATDWVHVATAEKNKVKAYVDV
                     DSIMEDGNKRRFWMYHDFKGVQSKDGNSIEKAFGYDVVDCKDKTILYVELIIERADGS
                     KTKSKLKTEVESVRPNSVDEGILEFVCNYKKEG"
     gene            667..1302
                     /locus_tag="VI935_09245"
     CDS             667..1302
                     /locus_tag="VI935_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25821.1"
                     /translation="MKKKPEGRQLILSMIVVLMYFMGCDNPFDEGPSSQPDFEAGATG
                     SNDITDVFIEINASRDSGDVSGDGVVVIGDEQFTLDVVDATLSTDFGMEDPIPQTDNL
                     LVGVDFVCTPAFAELTVVLSPGESVTTIRLNECEKDNPDEFEEEGVIINPCTGEEVTD
                     IEQFRGVCIAEQFGGESFQDTIVLNEFNGHCAPDDPCVVPIDVGIFVPELQ"
     gene            1364..2551
                     /locus_tag="VI935_09250"
     CDS             1364..2551
                     /locus_tag="VI935_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLE25822.1"
                     /translation="MEIKDIEFEEGSSGINELREFIQEDKGAFFSLEIEDGFKPHAIE
                     SELEPQQEPEFIEHEEKQRVPDEWIRLLSVYFKDVASESLLTPNEVMEISVKIEKCEA
                     RAREIKALIYELSKERVDKSRKNGHKIGSNGKGVSERIHRLNSLIKAYRERAKELRKR
                     FVKANLRLVLSIANRYTGRGLPLSDLIQEGNLGLMRAVERFDYTMGYKFSTYASWWIH
                     QAISRALLGQTKTIRIPTYVLEQASKVHRISSILQDKKTGRKPTPEEISERAGISVKI
                     VKRVLEAGNEVVRLDSPILDGEKITLLEFIPDNRLPLPDSVVAKVMLPQIIREALLKL
                     TPREKKIIRMRFGIDHDATHTLDEIGREFNVTRERIRQIEKRALEKLATSRIGEVLKD
                     FLE"
     gene            2582..2923
                     /locus_tag="VI935_09255"
     CDS             2582..2923
                     /locus_tag="VI935_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25823.1"
                     /translation="MKRIFMFITATVVLSTILFFSHELNTNAASSPKYMIGEVTLVDE
                     RMFIVKEDVTQATYELTASPAKLRALNTGYRIEVTATDGKVLSLTILGMPMQAESEPY
                     QKWKVIKPPEG"
     gene            complement(3046..3960)
                     /locus_tag="VI935_09260"
     CDS             complement(3046..3960)
                     /locus_tag="VI935_09260"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HLE25824.1"
                     /translation="MFKRYSSNLAVSKILRIFGHAKFPVKRHCLRCHSRRLSRLSDGR
                     FFCRRCHFKFSLLTNTYLQRSFLPLDQWYELLWWFAYEFTANKTAKEVRSPQQRIHSC
                     FSLIRKAIYNYEQSRMNQFHGEVEVDETYVGPKFKNRRKEKREYYRKINAVRRGRGAK
                     ILNQPVFGLYQRDGQVYVEFVTDVKKKTLQDIIKGRIVLESEVYTDTWKSYRGLKRQG
                     YHHEVIDHGKEEYLKEKRGKKIHINGIEGFWGYLKEHILKHHGVAKDNLIYYIKEQEF
                     RFNHRYLTTDELVQKLVEIIVKFGPREA"
     gene            complement(3978..4403)
                     /locus_tag="VI935_09265"
     CDS             complement(3978..4403)
                     /locus_tag="VI935_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25825.1"
                     /translation="MSGRLGLVFESPFSIGQEEIPVSIVDQSHGTRFSIEEIRALFVP
                     DASLEYTEGFTINEIWRQAGIEFRLVTTVDHMIESHLADFMPRDSSIEVGYARRFNRP
                     GALNIYFVRDLERNRGIASLYVPNEKVDNSYGHYRFHGP"
BASE COUNT         1430 a          785 c         1040 g         1224 t
ORIGIN      
        1 tagagaaatg gttacaggac aggctttaaa aatagaaagg gggagaaaaa aatgaaaatc
       61 aaaaaaataa ttctagcttc ttttttaagc ctagcgcttc ttgcaagcta cggatgtgca
      121 tttcttgtag gagcagcagc agggggtgca ggcggatata tcctaaggga tcagggctat
      181 gaggtacagt ctccggttga aaaggaagga gggtaacgta atggggaaaa aaatatttat
      241 ggtttggata tcggtgatag tattttttat gatattttca aatgctaggg caacagactg
      301 ggtacatgta gcaacggctg aaaagaataa agtaaaggcc tatgtggatg tggatagtat
      361 aatggaagat ggtaataaga gaaggttttg gatgtatcat gattttaagg gagttcaatc
      421 taaagatgga aattccatag aaaaggcctt cggttacgat gttgttgact gtaaagataa
      481 aaccatactg tatgtggaac ttattataga acgtgctgat ggaagtaaaa cgaagagcaa
      541 attaaaaacc gaagtagaat cagtaagacc taatagtgta gatgaaggta ttttggaatt
      601 tgtctgtaat tataagaaag aaggataaga atcgagctca taagaagagc cttggagcaa
      661 ggctaaatga agaagaaacc ggaaggtaga cagctaattt tatccatgat tgtagtgtta
      721 atgtatttca tgggatgcga taatcctttt gatgaggggc ctagctctca acctgatttt
      781 gaagctggtg ctacgggctc taatgacatc acggatgtgt ttatagaaat taatgcatca
      841 agggactcgg gcgatgtgag tggggatgga gttgtggtta taggagacga gcagttcact
      901 ttggatgtag ttgatgcaac cctctcgacg gattttggga tggaagatcc tataccacag
      961 acagacaatc ttttagtggg ggtagatttt gtctgtacgc cagcatttgc agagctaaca
     1021 gttgttttgt cccccggaga atcggtaaca acgatacggc ttaatgagtg tgaaaaggat
     1081 aatcccgacg aatttgaaga ggaaggagta atcattaacc catgcacagg agaagaggtt
     1141 actgatatag agcagtttag aggagtatgc atagctgagc agtttggagg tgagtctttt
     1201 caagatacta tagtgcttaa tgaatttaat gggcactgcg ctccagatga tccctgtgtt
     1261 gttcctatag atgttggaat ttttgtacct gaacttcaat agttcagacc catacttcat
     1321 tgattagatt tttaatcaat taataagcaa agggggtaaa aaaatggaga taaaagacat
     1381 agagtttgaa gaagggtcct caggaataaa tgagcttaga gaatttatcc aagaagacaa
     1441 gggggctttt ttttctcttg agatcgaaga tggtttcaag cctcatgcga ttgagtctga
     1501 actagaaccc caacaagagc cagagttcat agagcatgaa gagaagcaga gggttccaga
     1561 tgaatggatt agactccttt ctgtttactt taaagacgtg gcgagtgagt ctcttcttac
     1621 accaaatgag gtaatggaga tttcagtaaa gatagagaag tgtgaggcaa gagcaaggga
     1681 gataaaggca cttatttatg aactttcaaa agaaagagtt gataagagca gaaaaaacgg
     1741 acataaaatc ggtagtaatg gaaaaggggt atcagagcgt atacataggc taaattcact
     1801 cataaaggcg tatagggaga gggcaaagga acttagaaaa aggtttgtga aggcgaatct
     1861 aaggttagtt ctgagcatag caaatagata cacaggtcga gggcttccgc tatcggatct
     1921 tattcaggaa gggaatttgg gtttgatgcg agcggtagag agatttgatt acacgatggg
     1981 gtataagttt tcaacatatg cttcatggtg gatacatcag gcaatatcta gggcgctgct
     2041 gggtcaaaca aaaacgatta ggattccaac atatgtgtta gaacaggcga gcaaggtaca
     2101 caggataagc tcaattcttc aagataagaa gacaggcaga aaacctacac ctgaagaaat
     2161 ctccgagagg gcaggaatct ctgttaagat tgtaaagcgg gttttagagg caggaaacga
     2221 agttgttcgt ctagactcac ctatactcga tggggagaaa ataactcttc ttgagtttat
     2281 acctgataac aggctccctt tacctgattc agttgtggca aaagtaatgc ttcctcaaat
     2341 tataagagag gctctcttaa agcttactcc tagggaaaaa aagataatta ggatgagatt
     2401 cgggatagat catgatgcca ctcatactct tgatgagata ggaagagagt ttaatgtcac
     2461 gcgtgagcgt ataaggcaaa ttgaaaaaag agcgcttgaa aagcttgcaa catcaaggat
     2521 cggtgaagtc ttaaaggatt ttcttgaata agaaacaaca aatataagga ggtaacataa
     2581 gatgaaaaga attttcatgt ttataacagc aacggttgtt ctcagtacca ttttgttctt
     2641 tagccatgag ctcaacacaa atgccgcaag ttctccaaaa tacatgatag gcgaagttac
     2701 tctagttgat gaaagaatgt tcatagtaaa ggaagacgtt acacaagcga catatgaact
     2761 tacggcttct ccagctaaat taagagcatt aaacacagga tacaggatag aagtcacagc
     2821 gacagacgga aaggttttat cgttgactat attgggtatg cctatgcagg ctgagtccga
     2881 gccttatcag aaatggaagg ttattaaacc tccagaagga taatgtgttc ttgtagtgtt
     2941 gtaaaacagc agctaagtat aaaatctagg ttaataatcc acgaactatc tgaaggtagg
     3001 tgttagactc gagtttttag agttcgttga ctttagtata gggcattacg cctctcgcgg
     3061 accaaattta actataattt caactaattt ttgaaccaat tcatcagtag ttaaatacct
     3121 gtgattgaac ctaaattctt gttctttgat gtagtatatc agattatctt tagccactcc
     3181 atgatgctta aggatatgct ctttgagata gccccagaat ccttctatgc cgttaatgtg
     3241 aatcttctta cccctctttt ctttcagata ctcctctttg ccatgatcaa taacttcgtg
     3301 atgataacct tgcctcttta atccccgata agatttccag gtatctgtgt aaacctcgct
     3361 ctcaagaaca atcctgccct taatgatgtc ctgaagagtt ttctttttaa catctgtgac
     3421 aaattctacg tagacttgac catctctctg atatagacca aagaccggtt gattcagtat
     3481 cttagcacct ctaccgcgtc ttactgcatt gattttgcgg taatactctc ttttctcctt
     3541 tcttctgttc ttaaatttcg ggccaacata agtctcatca acctccactt caccgtggaa
     3601 ctgattcatc cgggattgct cataattgta aatagctttc ctaattaaag agaaacaact
     3661 atggatcctc tgttgaggtg atcttacttc ttttgcagtc ttattagctg taaattcgta
     3721 agcaaaccac cacagaagct cataccactg atctaaagga aggaagcttc gttgaagata
     3781 agtgttggtc agcagagaga atttgaaatg gcagcgacga cagaaaaacc gaccatcgga
     3841 caaccttgat aatctacgag aatgacaacg caagcagtgt ctttttacgg ggaattttgc
     3901 gtgtccaaaa attcgtaaaa ttttggacac ggctaaattg ctggaatacc ttttaaacat
     3961 ttcacaaaac cattatacta tggtccgtga aatcggtaat gcccatagct attatctact
     4021 ttctcgttgg ggacatacaa gctagcaata cctcggtttc tctccaagtc gcgaacgaaa
     4081 taaatattca aggcacctgg tcggttaaag cgtcgagcat agccgacttc gatggagcta
     4141 tccctgggca taaagtcggc aaggtggctt tctatcatgt ggtcaacggt agtgaccagc
     4201 cggaactcaa taccagcctg acgccatatt tcattgatag taaacccttc ggtgtattct
     4261 agggaagcgt ccggcacaaa cagagcccgt atctcttcaa tagaaaacct cgttccatga
     4321 ctttggtcaa ctatggagac aggaatctcc tcctgcccta tcgaaaacgg ggactcaaag
     4381 acgagaccca gacgcccgct caaacgcaag ctcgcgcgac gggcaacata gcgcgtgcca
     4441 aacatccggt gggcgactac aggtatggag aagagtatc
//
LOCUS       DATDRL010000278         5575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3522335, whole genome shotgun sequence.
ACCESSION   DATDRL010000278 DATDRL010000000
VERSION     DATDRL010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3522335"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VI935_09270"
     CDS             <1..438
                     /locus_tag="VI935_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HLE25826.1"
                     /translation="TDEKLAKAKELGADETVNYNKTDFAEEVKKITNKTGVDVIFDQI
                     GASIWDKNIQSLAPRGRLLLVGVVGGGAASANLGALIMRDISVLGVTVFNAARKELSN
                     VVNLTSEGKLRPVIDKAFRLDEAAVAQKLLEDRNQFGKVILNP"
     gene            complement(467..1297)
                     /locus_tag="VI935_09275"
     CDS             complement(467..1297)
                     /locus_tag="VI935_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HLE25827.1"
                     /translation="MTTGIFYSHIFKICPGPVVGPKFINFPEVLGDVLKLPNVKLFEH
                     EPVLEDLLLKFHTEEMIESLKNRPYGKNAFYSVGACVTASKKIMSGELKNGLLFNSAS
                     GHHAGPKQAWGGTYLNCVAPAVYTIRESMGVNKFSLLDTDCHHGDGDREMFKHDSSLL
                     HVCFCDSSYADETNICVGVGWRSNNNDYLDLVRKEFIPRAKKFMPAMIFHFLGHDTCE
                     GDYGDIGLTQDFFIELVKEVKECAEDVCHGRYLVITGGGSRRDVAEYIFPRIANLLAS
                     "
     gene            2281..2943
                     /locus_tag="VI935_09280"
     CDS             2281..2943
                     /locus_tag="VI935_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008272516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HLE25828.1"
                     /translation="MKPKSNKANYHHITLLGDSILDNGAYVQPGEPDVITQLRGKLPE
                     EWQATLRAVDGSITDDVKGQVRGLPEDASFLIVSVGGNDALSHISIFNQNAQSTAEVL
                     QRLADIRDKFQRNYQSMLKTVLSCNLPTAICSIYYPRFPDPALQRVAIVGLSVFNDCI
                     IYETFRFRLPLIDLRLICNEDRDYANEIEPSVHGGEKITDAILKVAKEFDSRKTRTVV
                     FT"
     gene            complement(3013..>5575)
                     /locus_tag="VI935_09285"
     CDS             complement(3013..>5575)
                     /locus_tag="VI935_09285"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HLE25829.1"
                     /translation="LLAHKFLSLVKNGFNPEQILCLTFTDKAKREMEARIINLLFDNA
                     IPCELSKLNIYTFHSFALKNIEEQNIISSNLLRFSIYTYLKDNQIFNYSDSYLIDTIV
                     PRIENLIRYLKSFGITPDKIDIEKTKSFLEGSDRIGKDEIDKYAEYFLQIFEYYEKSK
                     AGKGLDYADLLIRFLAERTMPQFEVVLVDELQDVNKMEADIALKSAKQFIAVGDKKQA
                     IFGFQGGTIINFKHFENSTQFILSENFRSTDQILKFASEHFVSKTREISHREELKGLK
                     NAEGKTGGKPVIYEVNKDSIPVAARALVQNLMRTTESLAIIARTNYQIMDIARELNSS
                     GINFSTTFFTASTEAKANIIKFLKGVLSNNIADIKNAMFTPFFPVSLQEAFGISEIKD
                     LTIDLFYQRCPEFQKLRSSVQNVEDVNTLFHEKIIPISLTYGEEYLSAAIKLQEAYKE
                     ALNVIEPKTINNLMVFLDTSDLLTDEFDRENKVMLTTVHKAKGKEFDTVIYVPTKTMD
                     RSSFQDEVVEAILKCNGIDVKEELEEESLRINFVAITRAINRLYIVTDRTLDFLNDHA
                     VSDVLLAPSGEESEFIESRKKAYACFLSGEFDKAKELLNAKKKWLLDYVKRYFESLNS
                     ISFSALSDSAYDYFVNSILRVSEYSPALTTGSEVHAIAERIVNGTECVVSEELLPFRK
                     NIEFLCSKISAGYPELLTTEYRFKIPLSEIIETDEKVFFSGYIDAIFKNKDNYLIVDW
                     KTDRNQDNDAKHRQQLEAYKRAFCVAKKISPDKVKVAIGFIGLRTAINTGRVEAFLDD
                     RQPQKSAFDTFSKKVNTILEWKKDPNLFFEKLIDEKEVDDVLWRSIVEQYRVETM"
BASE COUNT         1634 a         1124 c         1041 g         1776 t
ORIGIN      
        1 actgatgaga aactcgctaa agcaaaggaa ctaggggcag atgaaactgt aaattacaat
       61 aaaacggatt ttgcagaaga ggttaaaaaa ataactaata aaaccggagt ggatgttata
      121 tttgatcaga ttggtgcatc gatatgggac aaaaatattc agtcacttgc tccaagggga
      181 aggttactcc ttgtgggagt tgtcggtggt ggtgctgcct cagctaatct aggggcactt
      241 atcatgagag acatatccgt ccttggagtt actgtattta atgctgcacg aaaggagtta
      301 tcaaacgttg ttaatttaac ctccgagggt aagcttcgtc ctgttattga taaagcgttt
      361 aggctagacg aagctgctgt ggcacaaaag cttcttgagg acagaaatca atttggtaag
      421 gttattttaa acccctgacg ggattgaatt taagtttata tcttttctaa cttgctagta
      481 gatttgcaat tcgtggaaag atatattccg ccacatctcg tcgcgaccct cctcctgtga
      541 ttaccaaata tctgccatga caaacatctt ctgcgcactc ttttacttct tttacaagct
      601 ctataaaaaa gtcctgcgtc aatccaatat cgccgtaatc cccctcacat gtgtcatgtc
      661 cgaggaaatg gaagatcatt gctggcataa attttttagc tctcggaatg aactcttttc
      721 tgacaagatc aaggtaatca ttattgtttg aacgccagcc aacgcccaca cagatatttg
      781 tttcatctgc atagctcgaa tcacaaaagc acacgtggag aagggatgag tcatgtttaa
      841 acatttccct atccccgtct ccatgatgac agtctgtatc aagtagggaa aatttattta
      901 ctcccatgct ttctcttatt gtgtaaacag caggcgctac acagtttaga taagttccac
      961 cccatgcttg tttgggtcct gcatgatgcc ccgatgcgct gttgaataga agtccgtttt
     1021 ttagctcacc gctcattatc tttttgctag cagtaacaca tgccccaact gaatagaatg
     1081 cgtttttgcc atacggcctg ttcttaagac tttctatcat ttcttccgta tgaaatttta
     1141 gaagtaaatc ttccaacaca ggttcatgct caaagagctt aacattgggt aatttaagta
     1201 catctccaag aacctcaggg aagttgataa acttaggccc aaccactggc ccagggcaga
     1261 ttttgaaaat atggctatag aatataccag tcgtcatcta cttttggtga acttctgtaa
     1321 catcatatag tatcatcggt taattttaat tcttttaact ttacttgcaa cccctcaaag
     1381 agtagccttc ccggatctcc ttaggtagtt atcaagaggg agttttctag gaaaccgcac
     1441 gcttgctggt tggcaaaccg catgtccacc ccatttcttc cctccttttg taaaggaggg
     1501 tcagggtgga ttttatttcc caccttactt tctgtcttta caaaagtatg gtatacccac
     1561 ataacataaa ttactctaag gaaaccaatg agggatttca ctttaatttc aatattcgaa
     1621 agggagtgaa taacgacgcc aatagctcaa cttactggaa ttagaggtgt ttatcttgtt
     1681 gctgctgaat tgtctcgact tggttttatc gtttcaccag catcccgtag ctctatcggt
     1741 gctgatttac tagttacaga ccagaaatgt aaacgtgctt attcagtcca agtaaaaaca
     1801 aatgcaagga cgtttaattt ttggctgctt aacccaaaag cgagggagat tgtatcacct
     1861 acgcatatat atgtttttgt taatctacgt caaggaggga atgatatcga gtattttgaa
     1921 gtgcctagta gacaagtctc taataggatg gtggtacaaa gaaggccaag tccaacatgg
     1981 tattcattta gtattgagga tgcggaacca tttcaaaata gatggaaagt ttttgattct
     2041 ccataattat tagcttccca gatacctcgt cataggtaag acctagtaaa aacagagcta
     2101 cattatattc gaccatcatt cacagcagaa actcaaacca tttatgccag ctaacttacc
     2161 ctttacaaaa gtaatttccg ttgactttag ttattaactt tgcgatcaat agctatatga
     2221 acagagcaag tgatatattc attttgatta taagtcttaa ttatttataa ttctatgcca
     2281 atgaaaccga aaagtaacaa agctaattat catcatatca ctcttcttgg cgattccata
     2341 ctggataatg gagcatatgt ccaacccgga gagccagatg ttataacaca gctaagaggt
     2401 aagcttcctg aagagtggca ggcaacgcta agagccgtgg atggaagtat cactgatgat
     2461 gtaaagggac aggttcgtgg actcccagaa gatgctagtt ttctaatagt tagtgttggt
     2521 ggcaatgatg ctcttagtca tatttccatt tttaatcaaa atgcacagtc aaccgctgaa
     2581 gttctacaaa gactcgcaga tatacgagat aaattccaac gtaattatca gagtatgtta
     2641 aagactgttc taagttgtaa tctaccaaca gctatctgtt caatctatta tcctcgattt
     2701 ccagaccctg ctttgcagag ggtggctata gttgggctat cggtattcaa tgactgcatc
     2761 atatatgaaa cctttagatt tagattgcct ttgattgatt taagactgat atgtaacgaa
     2821 gacagagatt atgcgaatga aattgagccg tcggtgcacg gtggagaaaa gattactgat
     2881 gcaatcctga aagtagcaaa agaatttgac tcaagaaaga ccagaacggt tgtttttact
     2941 taagggggaa tctcaaagaa acagatatca tcgtccaaat taatcctgcc gggttaggta
     3001 gcgtcctatt cattacattg tctcaactct atattgttca acaatgcttc tccatagaac
     3061 atcgtcaact tctttttcat caataagctt ctcaaagaaa aggtttgggt cttttttcca
     3121 ctcaagaatg gtatttactt tcttagagaa tgtatcaaaa gcagactttt gaggttgtct
     3181 atcgtcaaga aaagcctcta ctctgccagt atttattgca gttcttagtc caataaaacc
     3241 tattgctacc tttactttat cggggctgat cttcttagca acacagaaag ctcttttata
     3301 agcttcaagc tgctgacggt gtttagcatc attgtcctga tttctgtctg ttttccagtc
     3361 aacgatcagg tagttatctt tatttttaaa aatagcgtca atataaccac taaagaaaac
     3421 cttctcatcg gtttcaataa tttccgaaag aggaatctta aatcggtatt ctgtggtcaa
     3481 taattccgga tatccagcac ttattttact gcacagaaac tctatgttct ttctaaatgg
     3541 caataactct tcagaaacaa cacattcagt cccattcaca atcctttcag caatagcgtg
     3601 cacttcactg cctgtggtta aagccggaga gtattcgctg actctaagaa tagagtttac
     3661 aaaataatca tatgcactgt cagacagagc tgagaaagag atactattta gagactcaaa
     3721 ataacgtttt acgtagtcta agagccactt ctttttagca ttgaggagtt ctttagcctt
     3781 atcgaattcg ccactaagga aacaggcgta tgccttcttt cttgactcta tgaattcgga
     3841 ttcttcaccg gaaggtgcca gaaggacatc cgacacagca tgatcattca ggaaatctaa
     3901 agttcgatca gttactatgt aaagcctgtt aatcgcgcgc gtaatggcaa caaagttaat
     3961 tcttaagctt tcctcctcaa gctcttcttt aacatctatg ccgttacact tcagtattgc
     4021 ttctaccacc tcgtcctgga aactcgacct gtccatggtt ttagttggaa cgtaaattac
     4081 cgtatcaaat tcttttcctt tcgccttgtg cacagtcgta agcattacct tgttttctct
     4141 atcaaactcg tctgttaaca aatcggaagt gtcaagaaaa accatcagat tattgatggt
     4201 tttgggctct atgacattaa gcgcttcctt ataggcttct tgcagcttta tagccgctga
     4261 taagtattcc tcaccgtagg ttaatgatat tggaataatc ttttcatgaa ataatgtatt
     4321 aacatcttca acattctgga cagagcttct cagcttttga aactccggac atctttgata
     4381 aaacaagtct atggttaaat ctttgatttc tgaaatccca aaagcctcct gtaatgaaac
     4441 agggaagaag ggtgtaaaca ttgcattctt aatatcagca atgttgttac tcagcactcc
     4501 cttcaagaat tttatgatat tagcttttgc ctctgttgat gcagtgaaga atgtggttga
     4561 gaaattgatg ccactgctat ttagttctct ggcaatatcc ataatctgat agtttgttct
     4621 agctatgatt gccagacttt cagtagttct cataagattt tgaaccaaag ccctggcggc
     4681 aacgggaata gagtctttat ttacctcata tataaccggt tttccccccg ttttgccttc
     4741 agcatttttc aaacccttta gttcctctct gtgagatatt tccctggttt ttgatacaaa
     4801 atgctcactc gcaaacttaa gaatctggtc tgtgcttcgg aaattctcgg aaagtataaa
     4861 ctgtgtagag ttttcaaaat gcttaaagtt aataattgtc cctccttgaa agccaaaaat
     4921 agcttgcttc ttatctccca ctgcaataaa ctgtttggca gatttgagtg ctatatcagc
     4981 ctccatcttg tttacatctt gaagctcatc tacaaggacg acctcgaact gcggcatggt
     5041 tctctccgcc aggaatctta ttaacaagtc ggcataatcc agccccttac ccgccttact
     5101 tttctcatag tattcaaata tttgtaaaaa atactcggcg tatttatcga tttcatcctt
     5161 tccgattcta tcgcttccct ccaaaaaaga cttggttttc tcaatgtcta ttttatccgg
     5221 agttatgccg aaacttttca agtatcttat gaggttctct atcctgggga ctattgtgtc
     5281 aatcaaatag gagtctgaat aattaaaaat ctgattatcc ttcaaataag tgtatatgga
     5341 aaaacgcaag aggttagagg aaataatatt ctgctcttcg atatttttta gagcgaatga
     5401 gtgaaatgtg tatatattga gctttgataa ctcacatgga atggcgttat caaataggag
     5461 gttgataatc ctcgcttcca tctccctttt agctttgtct gtaaaggtta ggcagagtat
     5521 ttgttcagga ttgaaaccat tttttaccaa tgataggaac ttgtgggcaa gtaag
//
LOCUS       DATDRL010000279         2724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_293450, whole genome shotgun sequence.
ACCESSION   DATDRL010000279 DATDRL010000000
VERSION     DATDRL010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293450"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VI935_09290"
     CDS             <1..377
                     /locus_tag="VI935_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25830.1"
                     /translation="EEVETLEAAPAQSEEEDLEESSEEERKEEAIRQFWRSRALEIEA
                     EEKLILEEIEITKKLMSAKKGEVDFLLINGYFADYSILELRNLQDYLKGLQDQLSLFK
                     EEKAQLKDEARRAGVPPGYLRP"
     gene            385..759
                     /locus_tag="VI935_09295"
     CDS             385..759
                     /locus_tag="VI935_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HLE25831.1"
                     /translation="MKRIKIIAGEIEVLANLHETKTASAIWDSLPFRGKVNRWGDEIY
                     FEIPLKLPKEKEAKEAVLMGELGYWPVGHAFCIFFGMTPVSTENEIKAASPVNIFGRV
                     EGNPEILKKVPEGSEIKVEKLS"
     gene            1100..2134
                     /locus_tag="VI935_09300"
     CDS             1100..2134
                     /locus_tag="VI935_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25832.1"
                     /translation="MIPYINKAIELSRELKLTTSHHPWEIQHGILANGNDYRIYDPWG
                     KEINALDFLLRGTNVEWYPIPGGIVREDEDGYPYFIEGERGLEAEIHQNLFLGEFSTL
                     GIDLDSTTVLTNLENEYPLRKALERAMLYTFPEKLKESKEEPSWLLTSFSRYLPMGSW
                     KNINGDKVRLEDIVEYANELELGFGSCFGTHVMEGIAVALIKYMEKTEIPPLKFQSPW
                     REAHEKLELGINLVKKLQREDGSIPRLWFKPDPIPRNLNEFKLMIEQLIGITLLRPRE
                     ILYPTGHTLDWLVQYVPAQMLSQDWVVRAVEITARTIADNFHKFLKEVSSLTHAVHAL
                     KVYKDKLGKS"
     gene            2146..2493
                     /locus_tag="VI935_09305"
     CDS             2146..2493
                     /locus_tag="VI935_09305"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HLE25833.1"
                     /translation="MKPKEISEFSDTALLIVWDDGHESIYLYEDLRQSCPCATCNDIR
                     QSIESGRKGKTLFKRSIPLGTSIQNLLIRPDKIDPVGLYAIRFKWNDGHETGIYSFDF
                     LRKLCMCEKCRPN"
BASE COUNT          930 a          503 c          616 g          675 t
ORIGIN      
        1 atgaggaagt ggaaacgtta gaagcggctc ctgctcaatc ggaagaggaa gaccttgaag
       61 aaagctcaga agaggagaga aaagaagaag ctataaggca attttggaga agccgtgcac
      121 tagaaataga ggctgaagaa aaattgattc ttgaggaaat tgaaatcact aagaagctaa
      181 tgagtgcaaa gaaaggggag gttgatttct tgcttataaa cggatacttt gcggactatt
      241 ctatactaga acttagaaat ctccaagatt atctaaaagg gcttcaagac cagttgagct
      301 tattcaaaga agagaaagca caacttaaag atgaagcccg ccgagcggga gtcccaccag
      361 gatatttgag accgtaaaat atagatgaaa aggattaaga ttatcgcagg tgagattgag
      421 gttttagcaa atctacacga gactaagact gcatcagcta tatgggattc actgccattt
      481 cgaggaaaag ttaatagatg gggagatgaa atctactttg aaattcccct aaaacttccc
      541 aaagaaaaag aagcgaaaga agccgtacta atgggagagc tcggatactg gccggtaggc
      601 catgcgtttt gcatattctt tgggatgacc ccggtaagca cagaaaatga aataaaggcg
      661 gcaagccctg tgaatatatt tggcagggtg gaaggaaacc cagagatatt aaagaaagtc
      721 ccggagggtt cagaaattaa ggttgaaaag ctttcctaat tcaaaaagct acaaaaaaaa
      781 agagctacac tgcagaaatc tgcagtgtat catcatatga acatactgtc attttggtgc
      841 gaggagcgag tgggccaaaa ccaagaatgt agcgaaaggg acaagcaatc taaaacaaag
      901 attagattgc ttcatttcat tcgcaatgac aaatctggcc tgtcattgtg aggaagtgag
      961 gagcatttct cccgcagtgt gggagaaatt gtagtaaaaa aataacgaag caatcaaaac
     1021 acaaaagaga gatttgttac ccgcaatgac cgttacaacc atgtagcaag ctgtaggtaa
     1081 ctatttcgcg aaaacgatta tgatcccata tataaacaaa gcgattgaac tttcaaggga
     1141 actcaagctg acaactagcc atcacccttg ggaaattcag catggcattt tagcaaacgg
     1201 taacgattac agaatctatg acccatgggg caaagaaatc aatgccttgg attttctcct
     1261 tcgaggaaca aacgttgagt ggtatcccat tcctggaggc attgttcggg aagatgaaga
     1321 tggttatcca tatttcatag aaggagaaag gggactcgaa gcagagatac accaaaatct
     1381 attcctagga gagttttcta cactaggtat agaccttgat tcaactactg ttctaaccaa
     1441 ccttgaaaac gaatacccac ttagaaaggc tttggagaga gccatgcttt ataccttccc
     1501 cgaaaagttg aaagaaagta aagaagagcc aagttggttg cttacctctt tttcaagata
     1561 cctacccatg ggttcatgga agaacattaa cggagataag gtcagactcg aggatatagt
     1621 cgaatacgct aatgaattag aactagggtt tggctcgtgc tttggaacgc acgtaatgga
     1681 gggtatcgca gttgctctaa taaagtatat ggaaaagact gaaattcctc ctctgaagtt
     1741 tcaaagccca tggcgggagg cacatgaaaa gcttgaacta ggaattaatc tagtaaaaaa
     1801 gcttcaacga gaagacgggt ctattccacg cttgtggttt aagcctgatc cgatcccaag
     1861 aaatctaaat gagtttaaac tcatgataga acagcttatc ggaatcacgc ttctaagacc
     1921 gagagagatc ctttacccca caggacatac gctagactgg cttgtgcaat acgtacccgc
     1981 ccagatgctt tctcaagatt gggttgtaag ggctgtagaa attactgcta gaactatagc
     2041 tgataacttc cataaatttt taaaagaggt ttcttctctt acacacgcag ttcatgcgct
     2101 caaggtttat aaagataagt taggcaagag ttagcgaatt ataaagtgaa gccaaaagag
     2161 atcagtgaat tcagcgatac tgctctatta atcgtctggg atgacggaca tgaaagtatc
     2221 tatctttatg aagacctcag acaatcatgc ccctgtgcca catgtaatga cattagacaa
     2281 tctatcgaaa gcggtaggaa gggtaagacg ctttttaaac gtagtatccc tcttggaaca
     2341 tcaatccaaa atctactgat aagacctgat aaaatcgatc ccgtaggact atatgctatc
     2401 aggtttaaat ggaatgacgg ccatgaaaca ggaatatatt ctttcgactt tctaagaaag
     2461 ctatgtatgt gtgagaagtg cagacccaac taaaaactat taagccacag aggtctcaga
     2521 gaaggatatt ttaagaaaaa attccgtgaa ctctgtgttc tccgtggctg agtatctaat
     2581 aaaatttcac tgtatctcaa atactatgtt taccgttgcg gtgactgtaa gttcacctgg
     2641 ttcaattggg gttggagcgg cctccatagc ggctaatttc tgagcaccaa attccctata
     2701 tagcggtctt ggcacctcat aaga
//
LOCUS       DATDRL010000280        11995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1148922, whole genome shotgun sequence.
ACCESSION   DATDRL010000280 DATDRL010000000
VERSION     DATDRL010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11995
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148922"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..1395)
                     /locus_tag="VI935_09310"
     CDS             complement(238..1395)
                     /locus_tag="VI935_09310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLE25834.1"
                     /translation="MISGLNKTQSKNFSLITIANFFLFCNFSSFFLLPLFIKKLGGNE
                     ANIGFIMGSFGVTSLGSIPIVAFLIDKYGRRRFMLFGAIVMFLSSLSYLFARELSPIF
                     YILRMLQGVGFAFFFTSAGTAVADFIPEMQRGQGLGIFGAFTIAAYALGPTIGEVIIE
                     NLGFNLFFTYASSFSLIAIVLVYKAEGAYFKRSHDPYGFDFFRLAFSRRYSVPLLSNL
                     ILAGGFGSVLNFISVFVRQKGLDVFYFFLVYAVTITSVRIIGGKVSDVFGRKKVASPS
                     LFVFSLSIAAMIFINSVYKLVLISFLFSLSYGMLYPTLSALMMDKANPDERGKAMGAF
                     NACFGIGTNFLAFAFGVIARDLGFTSMYLISAAFVFLGFLLFTLFEPQRDS"
     gene            1752..1825
                     /locus_tag="VI935_09315"
     tRNA            1752..1825
                     /locus_tag="VI935_09315"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1786..1788,aa:Lys,seq:ttt)
     gene            1953..2030
                     /locus_tag="VI935_09320"
     tRNA            1953..2030
                     /locus_tag="VI935_09320"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1989..1991,aa:Glu,seq:ttc)
     gene            2453..2707
                     /locus_tag="VI935_09325"
                     /pseudo
     CDS             2453..2707
                     /locus_tag="VI935_09325"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
     gene            2879..3217
                     /locus_tag="VI935_09330"
     CDS             2879..3217
                     /locus_tag="VI935_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25835.1"
                     /translation="MADKTFTNPLTLKTDTRITADRILECTEILRFQGLVPHKIKFGM
                     VDKCPECGEPTWGLFMAEDEGVEIGEGEPLGICLQCNATIFVDENGKPKARIISSQDL
                     LRGLRIGKGK"
     gene            complement(3239..3655)
                     /locus_tag="VI935_09335"
     CDS             complement(3239..3655)
                     /locus_tag="VI935_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25836.1"
                     /translation="MHKAMIVLLLTNLFCSMGRAEEIDIKNLMINHAEWYIKITDWHV
                     YGGWSCPAIIHQVTIENTSDIAYKDIQIRVLYYSTTYENYGTQISQETGVLPVIVLPH
                     SKETYLKSGATLGAGCMAMYAGYFEVLGATPASWGR"
     gene            complement(3828..4136)
                     /locus_tag="VI935_09340"
     CDS             complement(3828..4136)
                     /locus_tag="VI935_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25837.1"
                     /translation="MFYFVSFNPREGARREQIVEAYRRYARHFEKKVPQFKLVGFYSR
                     NALLGSRPHYLAIWEFSEYSDLDEWNKLFAKDKEGQKLAKALGDLATDWEAKVMSKLI
                     "
     gene            complement(4196..4843)
                     /locus_tag="VI935_09345"
     CDS             complement(4196..4843)
                     /locus_tag="VI935_09345"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HLE25838.1"
                     /translation="MQIIKLTMVKEVLLATRNEGKIKEFSTLLSPVFERVISLRELDT
                     VPDIIEDGETFKENALKKARIISGLTQKVTLADDSGLEVESLGGRPGVFSARYAGEKA
                     GDKENIKKLLKELSGVGDRRARFVCALALVFPDGLAGLTANGREIVVEGRCDGIIIDD
                     PRGESGFGYDPVFFLPQIKKTMAELTPEEKNRISHRALAVRKLITCLKKSRTHNE"
     gene            complement(4883..5311)
                     /locus_tag="VI935_09350"
     CDS             complement(4883..5311)
                     /locus_tag="VI935_09350"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HLE25839.1"
                     /translation="MKLMLHENGLSTFKRRASRIYPREIYGILLGKKLKNSTYKVLKI
                     VIPPVVESTYDYVIPDYNEIERIVFASGLDYIGSIHSHPQAAPTVSAHDYQYWDKNKD
                     EVVGILSIRKRNSYKVTELKFWRKNSSLPVKFKTFKPNGE"
     gene            complement(5430..5786)
                     /gene="ssrA"
                     /locus_tag="VI935_09355"
     tmRNA           complement(5430..5786)
                     /gene="ssrA"
                     /locus_tag="VI935_09355"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(6131..6580)
                     /gene="smpB"
                     /locus_tag="VI935_09360"
     CDS             complement(6131..6580)
                     /gene="smpB"
                     /locus_tag="VI935_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HLE25840.1"
                     /translation="MEKVVCQNPKAHRDFFLEEKYEAGIELKGSEVKSLRDGQASLKE
                     GFAMIQGDEVFLINSYIAPYEGANRFNHDPKRERRLLLHRREINRLIGKTKIKGYTLI
                     PVRIYFKNRRAKVELALAKGKKAYDKREDIKRREAQREVDKALKKRY"
     gene            complement(6937..7125)
                     /locus_tag="VI935_09365"
     CDS             complement(6937..7125)
                     /locus_tag="VI935_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25841.1"
                     /translation="MPTQDGNKFHLENAPIGGVFQPRQTAQECPVPPAIMNRGFPSPK
                     DETRMSRLSRGDVNQFAL"
     gene            complement(7207..7656)
                     /gene="dtd"
                     /locus_tag="VI935_09370"
     CDS             complement(7207..7656)
                     /gene="dtd"
                     /locus_tag="VI935_09370"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HLE25842.1"
                     /translation="MRAVIQRVSEAKVEVDGDIIGKIGEGFLVLIGVRKDDTEEDVRY
                     LADKVLGLRIFEDEAGKMNLSITDVKGEILTVSQFTLYGDSRKGRRPSFDEAAPLDVA
                     EKLYELFVEEIRKSGIKVETGKFRALMDVHLINSGPVTILLDSKKLF"
     gene            7809..8129
                     /locus_tag="VI935_09375"
     CDS             7809..8129
                     /locus_tag="VI935_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25843.1"
                     /translation="MVKVLKVSLFLLTILIIPTSIKAETPSPEPGKRLITHIVKKGEE
                     LHLLAGYYLLNARDWYLIYNRNSDVIRNQNRIFPGQELMIYVDNDWQPPYNLDEYIKG
                     LGRE"
     gene            complement(8295..8843)
                     /locus_tag="VI935_09380"
     CDS             complement(8295..8843)
                     /locus_tag="VI935_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25844.1"
                     /translation="MRLCELEKEIKYAFYSQKISIEQAIILSEVPSSLRIEILNRVLL
                     KCKVNTNETREIVREIVEITARDKGSVKEIIDEIELKISQGDVKTDSFKRELKVMRYP
                     MLTRVEEEFKDCLKSLNVPKDLTINHPPFFEGNYIEIRMRIESAQRLSENFSYLESVL
                     KKGLIDKLLGIVKEGKQGDRSF"
     gene            complement(8825..9007)
                     /locus_tag="VI935_09385"
     CDS             complement(8825..9007)
                     /locus_tag="VI935_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25845.1"
                     /translation="MKIVFYENQERLGEMEKAELLLKFKRLCGLDQSELTQRVLPFLG
                     ILPSRKILKSICGSVS"
     gene            complement(9106..9264)
                     /locus_tag="VI935_09390"
     CDS             complement(9106..9264)
                     /locus_tag="VI935_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25846.1"
                     /translation="MKLDNIPLSKIDFDDETFRLGSSKDISLLLNSIKEVGVINPLVL
                     REKGINTS"
     gene            complement(9433..10407)
                     /locus_tag="VI935_09395"
     CDS             complement(9433..10407)
                     /locus_tag="VI935_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HLE25847.1"
                     /translation="MKTEVKVGIFFVLGLVILLIIFEFITEIPLFTKEYLFKTYFKSV
                     GELKEGNPVKLSGVEVGKISKIKIADGKIEVTLKVKKGTPVKNDSLATIKLTSLLGTS
                     YINLTFGSPESPPAEPGSILQSQEPADVSEILAKVESAVSSIESVFSDNGEKIGSILN
                     SLDTVLSGAARGEGTLGKLIKDDSLYNEARDTLANLNEISSDIKQGKGTLGKLVKDES
                     LYNETKETMANLGQLAKRLNSAEGTFGKLLKDDTLYNQATEAATNLNSILKRIDKGEG
                     TIGKLVTDDSLYYDAKDAVKKVERAIDTQEDLAPLTTIGTAFGILTVF"
     gene            complement(10404..11222)
                     /locus_tag="VI935_09400"
     CDS             complement(10404..11222)
                     /locus_tag="VI935_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLE25848.1"
                     /translation="MNIRTEISDIIRSDAESNYRLNAPVGIKITKLNKSFGSNHVLRD
                     LNLEVKPSETLVVIGKTGSGKSVLLRHIIGLEGPDSGEILINGMDVNDPEVRKKHRFA
                     MVFQSSALFNSLTVAENITLYLREHKVIKDENAVREIVSNTLNIVGLEGKENIMPSEL
                     SGGMKKRVAIARALAMNPDLILVDEPTSELDPMMARTIGDAILNLRKHVEVTQIVVTH
                     DIDLAYYIADRIAVLDEGQIIEVGTPQEIDRSANPLVRKFITTQFEREEGGRRL"
     gene            complement(11246..11974)
                     /locus_tag="VI935_09405"
     CDS             complement(11246..11974)
                     /locus_tag="VI935_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLE25849.1"
                     /translation="MLQLLMESIFWCKAALSNREKIFRQMLEIGNQTLPVAALISLFI
                     GGVLALQSGPTLAQFGIEENIGGIVGLSFVKELGPVMASVLVAGRVGSAMAAELGSMS
                     VYQEIDALKTMDINPIRFLVMPRFIACFIALPLLVIYMDVIGWFGGAIVSSVNSDIHV
                     PFSVYYRNLSELVDFSDVLNGLVKATVFGIIISTVCCYVGLKTKGGPREIGTSVTKAV
                     VLSFILILVFDYFITRLLILLNLD"
BASE COUNT         3585 a         2628 c         2116 g         3666 t
ORIGIN      
        1 aatcgcccga ttttatttcc atttcattcg gaatggtaaa taaaaaacaa agccatttca
       61 agaatcaatg taaatctcag ccactaaggc acaaaggcac ggagatctgt tgttaccact
      121 acgacacaac gacacaacac aaagaaaaga attaaataaa aaatttttca catcgtgtct
      181 tagtgtcgtt gtggttaatg ttctcttttg tatcttagtg tctttgtggc taaataacta
      241 cgaatcacgc tggggttcaa aaagggtaaa aagcaagaac ccgaggaaaa cgaaagcagc
      301 ggaaattaaa tacatactag taaaacccaa atcccttgca attaccccaa atgcaaatgc
      361 caaaaaattc gtaccaattc cgaagcaggc attaaatgct cccatagcct ttcctctctc
      421 atcaggattt gctttatcca tcattaatgc actcaaggtt gggtatagca tcccatagct
      481 aagactaaat aaaaaagaaa ttaagacgag cttgtatacc gagtttataa aaatcatcgc
      541 tgcaattgac agcgaaaaaa caaacaggct tggtgatgct accttctttc ttccgaatac
      601 atccgaaact ttacctccta ttatccttac tgacgtaatt gttacagcgt agacaagaaa
      661 gaaatagaag acatccaagc ctttctgtct tacaaataca gagataaaat tgagtacaga
      721 gccaaaccca ccagcaagga ttaagtttga aagaagtggt acagaatacc ttcttgaaaa
      781 ggctaaccga aaaaagtcaa acccataagg gtcatgggag cgtttgaagt aagcaccctc
      841 agctttgtat actagaacga ttgcaataag gctaaatgaa gaggcatatg taaagaaaag
      901 gttaaaccca agattttcaa ttataacctc tcctatagtt ggtcccaatg cataagcggc
      961 tatggtaaac gctccaaata ttccaagccc ctgtcctctt tgcatctcag ggataaaatc
     1021 ggcaacggca gttccagcag aggtgaaaaa gaatgcaaac cccacccctt gaagcatcct
     1081 aagaatataa aaaataggac ttagttctct tgcaaagagg taactaagtg aagacaaaaa
     1141 cataacgatg gctccaaaga gcataaacct tcgtcttcca tacttgtcta tcaagaatgc
     1201 aacaatagga atagaaccaa gagaggttac cccaaatgaa cccataatga acccaatgtt
     1261 ggcttcattc ccacctaatt tctttataaa tagcgggaga agaaaaaaag aagagaagtt
     1321 acaaaaaagg aagaaatttg caattgttat taaggaaaag tttttactct gtgttttatt
     1381 gagtcctgaa atcatcgggg ctataagttt acgtgcttag aaactattta gccacaaatg
     1441 aacaagctct aatggccaaa cctaaactgc catcacaaat ttccaaatca caaattccta
     1501 ttccctagaa tccataagcc agaattcggt aggattagat ctttcttcta actactgact
     1561 tctggctccc gacttctcat ttctgattta gtgcttgtga ttcgaaattt gtggttccat
     1621 ctctagctaa agaagatgga agagaaagta agagcggctt ccagtcgcga cggctttatg
     1681 caacacagca acaatcaaag ataaattttt gacaaaggtt aatatcttta tataatcttt
     1741 gcttttaggt tgagccgtta gctcaggctg gtagagcatc tgccttttaa gcagagggtc
     1801 gcaggttcga gtcctgcacg gctcatacga ttacgaattt cggtctgacc actttttcgt
     1861 gctcgctgag tcttcatcga gtataatcga gactcataaa tatcgtaaat ttaaaaatcc
     1921 gaaatccgta atctacaatt cgagataaaa gtgtccccgt cgtctagcta ggcctaggac
     1981 accgcccttt caaggcggta acaggggttc aaatcccctc ggggacgcca atcaccccat
     2041 agtaaagcct caaagttaat ttggcttgat tgatttttca aacagccagt cctgcctata
     2101 ggcttaacta gcttttaacc ctttacctac aagcatcaaa aaaatgtttt taaatcatca
     2161 agctaaacgc agagggtctt aaatcagccg agtaatggtt atctcattat caaaaattct
     2221 gggatcatcg tttggatgct cttaaacaat tgcttgaggg agaagaagaa tgagtgacat
     2281 aataaaacaa caggttaaaa agtcataaaa gcaaagaggg aaaaagtttt tcaggcatgg
     2341 gtacgcctga aattgttaaa aggtcgtttc ctccttggga aatgactgtt tcagatgcgc
     2401 aaattaacct ccgtattagt ggagtctttc gaacacagat gaagggggaa cagacaatac
     2461 accaacagtt atcggtgttt ataaaaagat tatatataca atgagcttct tgttttcact
     2521 tggtcttgag agagaggata gcctcgagac acttgtgacg gttgagttca aagacgtcaa
     2581 agccggtacg gaggttatta tcactcatca acgatttgca aatgttaaaa cgcggaacct
     2641 tcataaacaa ggttggaagg gttgcttaga taacctggct aaaagaatta accgacggga
     2701 ggaatgaaga aacctgcaac aatctatcga ggattatatc aagtcaaaat tcaatgttga
     2761 atgacaatgc tatttaggtc tttttctatt tcgtgcaata aagcattcca ttcataaatt
     2821 ttaaagtaat cacaaaagga aaaatattgt ataatcaaga aaaagtaatg gagataacat
     2881 ggcagataaa acctttacaa acccattaac tctaaaaaca gatacacgaa ttacagcaga
     2941 cagaattttg gaatgtaccg aaatacttag atttcaaggc ttggtacccc ataaaatcaa
     3001 atttggaatg gtagataaat gccccgaatg cggcgaacca acctggggac tatttatggc
     3061 tgaagacgaa ggagtcgaaa taggggaagg agaacccctc gggatttgtc ttcaatgcaa
     3121 tgccactata tttgttgatg aaaatggtaa accaaaggcg aggattatta gtagccaaga
     3181 tttactaagg ggattacgaa tcggcaaggg gaaatgatac ttttcattaa cttcaatttt
     3241 accttcccca agatgcagga gtagctccta agacttcaaa atacccggca tacatagcca
     3301 tacaccctgc tcctagagtg gctccacttt ttagataagt ttctttacta tgaggcagca
     3361 caatcacagg taaaactcct gtttcctggc ttatttgcgt tccgtagttt tcatatgttg
     3421 tcgaataata tagtactctt atttgaatgt ctttataggc tatatcactt gtgttttcaa
     3481 tagttacctg atgaattatg gctgggcagc tccatccccc ataaacatgc caatccgtaa
     3541 ttttaatata ccactctgca tgattaatca tcaggttttt aatatctatt tcttcagctc
     3601 ttcccataga acaaaataga ttagtcagta ataagactat catcgcttta tgcatatata
     3661 tcaccttctt atttaatttt aactaatatg gaatgcctgt cccaatacaa tcaatataac
     3721 acgcactgct gtatggccac tttcgttaat caaataaaat tttttattga gttaggattt
     3781 ctttgcatta tatgcagctt tgtacttatt tcgtcactta ttttcaacta aatcaatttt
     3841 gacataacct ttgcctccca gtcggttgct aggtctccaa gtgcctttgc aagcttttgt
     3901 ccttctttat ctttagcaaa caacttattc cattcatcca agtcagagta ctcagaaaac
     3961 tcccaaatgg ctaaataatg tggccttgaa ccaaggagcg cattacgtga atagaatcca
     4021 acaagcttga attgaggaac cttcttctca aagtgccttg cataccttct ataagcttca
     4081 actatctgct ctcttctagc tccttcccta gggttaaacg atacaaaata aaacattctt
     4141 tcacctcctt aaattggatt cgttaccatt acaaacttaa aatcaccaaa ggtgcttact
     4201 cgttgtgcgt tctcgacttt tttaagcatg ttataagctt ccttactgca agggccctat
     4261 ggcttatccg atttttttcc tctggcgtta gctcagccat agtttttttt atctgaggca
     4321 agaaaaacac aggatcgtac ccgaacccgc tctcgcctct tggatcatct ataataattc
     4381 catcacatct tccttctaca acgatttccc ttccgtttgc tgtgagccct gccaaaccgt
     4441 caggaaatac taaggcaagt gcacagacaa accttgctcg tctatcacct accccactaa
     4501 gctccttaag gagtttcttg atattctctt tatcacccgc cttctctcca gcgtatcttg
     4561 ctgaaaaaac cccaggacgt ccgccgagtg actcaacttc taacccggaa tcatctgcaa
     4621 gcgtaacttt ttgtgtaagt cctgaaatga tgcgtgcttt tttaagggca ttttctttga
     4681 aagtttctcc atcctcaatt atatcaggca ctgtatcgag ttctctcagt gatataactc
     4741 tttcgaatac aggactaagt agagtactaa actccttaat cttcccctca tttctagtgg
     4801 ctaataaaac ctcctttacc attgttaatt tgataatttg cattaatgta gagacgaccc
     4861 ggcgggtcgt ctctacatat cgtcattctc catttggctt gaatgtctta aatttaaccg
     4921 gaagagatga gttctttctc caaaacttga gctctgtaac cttataagaa tttcgctttc
     4981 ttatgctaag gattccaaca acctcatcct tgttcttgtc ccagtactgg tagtcgtgtg
     5041 ctgaaacagt gggcgcagct tgaggatgtg agtgtattga gccgatgtag tctaatccac
     5101 ttgcgaagac aattcgctca atctcgttat aatcaggaat tacataatca tatgtagatt
     5161 ctacaactgg tggaattaca atctttaaga ctttatatgt actatttttg agcttctttc
     5221 cgagcaaaat gccataaatc tctctaggat aaatacgact tgcacgtcgc ttgaatgtac
     5281 tcaggccgtt ttcatgcaac ataagcttca ttaaagactt ctccctttaa tttgctcttg
     5341 ttatgcactc tgtgaattaa ttggctggaa atctaacttt ataagttttt atgaggaaat
     5401 gtataaaatc aaggaaaaaa attatcatct ggtggaggcg ctgggaatcg aacccaggtc
     5461 cgaagaagag cctcaccctg cgtctacatg tttatcccat ctattagatg tcgcagtgat
     5521 aactccgata ggcaggattt tcaccaccag cttgttaaat atctcgtcaa cctgtgtcac
     5581 aagcaccacc tgtagacaag cccgtctagc tcacgcctcg caaaaaccca acgggcgagg
     5641 tttttggaga cgccgctgct ctaattaggc agcaagtgca ttgattggtt cggcacttat
     5701 tatgtgctct gccagtttta cgagttagca gaaactcgac atgcagcttg ggttatccca
     5761 ttccccgtcg aagccacatc gcccccactt gtcaaagaac cggtttataa tcatttagcc
     5821 actgatgaac acaaatttac actaaaatat gatacatgat aaataatacc aattgtatca
     5881 attaaaagtg cattggaaaa agttgtcatt gcgagcgtta gcgaagcaat cttgtctttt
     5941 tggagattac ttcgtcactt cgtttctcgt aatgacagcc ttcgttatac acagctaaca
     6001 catcgaattg atataattca tcaagttttt acgtgtatct tgcatcccgc ttattctcat
     6061 tcgtttttat acgtgtcaat tggcttcgct gaaatgcccg accactaccg gcattttcgt
     6121 ggttaaacat ttagtaacgc tttttcaatg ccttatctac ttccctttgg gcttcacggc
     6181 gtttaatatc ctcacgcttg tcataagcct tttttccttt agcgagcgcc agttcaacct
     6241 tggcacgcct gtttttaaaa taaatcctta caggtattaa agtataacct ttaatcttcg
     6301 tctttccaat aaggcgattg atttcacgtc tgtgaagaag tagccttctt tcccttttag
     6361 gatcatgatt aaacctattt gcgccttcgt atggagctat gtagctatta ataagaaaca
     6421 cttcatctcc ttggatcatc gcaaagcctt cttttaggct tgcttggcca tcacggaggg
     6481 atttaacctc agagcctttt agctcaattc cggcttcgta cttctcctct aagaaaaaat
     6541 ccctatgggc ctttggattc tgacatacaa ccttttccac attgttaaat atagctatgg
     6601 ggtgctgaaa atcaactacg gaataacgtt cttgtggtgt tagaaaactc cgatgcccaa
     6661 taacaggagg gacaggacat tcctgtcctg tatgccaggg ttggaaaact ctgactatcg
     6721 tggtatttgc aaagaaggag gacaggccta gttgtgtgct cagccgaagg gtcttgcacc
     6781 tgccctattt taggcaacca caaggattgt tcctacaatc gggaaacgat gtggagttta
     6841 cgcgcgccag taaagccggt gtgctaacca tagttgaatt atatggaagg ctgaagcctt
     6901 cctctacaat ttgtagcgga aaccttgagg tttccatcag agagcaaact ggtttacatc
     6961 tccccgggat agacgagaca ttcttgtctc gtcctttggg cttggaaaac cacgattcat
     7021 aattgcagga gggacaggac attcctgtgc tgtctggcgg ggttggaaaa ccccgcctat
     7081 cggtgcgttc tcaaggtgaa atttattacc gtcttgggta ggcaacgagt cttgctcgtt
     7141 gccatctacc taattcaaac ttgaaaatca accgcagact agataaaaga tagtttttga
     7201 caaaccctaa aacaatttct tactatccaa caaaatcgtc acaggtcctg aattgataag
     7261 atgcacgtcc atgagagcac ggaatttgcc cgtctcaact ttaatgccac tttttcttat
     7321 ttcctctaca aaaagttcgt atagcttttc agctacatca agcggtgcgg cttcgtcaaa
     7381 ggatggacgt cttccttttc tgctgtcacc atatagggta aattgagaga ctgttaaaat
     7441 ttctcctttt acatccgtaa tagaaagatt catctttcct gcctcatctt caaatatcct
     7501 caaccctaat accttatcgg ctaagtatcg aacgtcctct tcagtatcat cttttcgaac
     7561 cccaataagt acaagaaagc cttccccgat ttttcctatt atatctccat ctacttcgac
     7621 ttttgcctca cttaccctct gtattacagc tctcatttta gttacccctt atataacgta
     7681 cactttttag tttttctagt agttttacgg taaaccttga aaaatcaagt ttgagaaaga
     7741 cgttatttta ttactcatta aaaaccagta aaattgggtt actaaaactt ctaccaagga
     7801 ggcatcaaat ggtgaaagtt ctaaaggtat ctctgtttct tctcacgatt cttattattc
     7861 ccacatcaat aaaagccgag acaccttcac cagaaccggg aaaacgtctc ataactcata
     7921 tagtaaagaa aggtgaagaa ctccacttac tcgcaggata ttatttacta aacgcaagag
     7981 actggtatct aatatataat cggaattcgg atgttattag aaatcaaaat agaatatttc
     8041 ctggccagga acttatgatt tatgttgata atgactggca gccaccttac aaccttgatg
     8101 aatatataaa gggtttagga agagagtaaa taaacttcac atatgtcatt gcgaggagta
     8161 gaacgacgaa gcaatctgat aaagattcct cacggagcct gtactgagcc actgcgaagt
     8221 attctgaatg ataaatcggg catgttattt ctgtaaatga cgcctattaa aagttttacg
     8281 tataacaatt ataattaaaa agagcgatca ccttgtttgc cttcttttac tatacctaga
     8341 agcttatcta tcaatccctt cttaagaacc gattcaaggt atgaaaaatt ttcggataat
     8401 ctttgggcac tttctattct cattctaatc tcgatataat taccctcaaa gaaaggaggg
     8461 tgatttatcg ttaaatcctt tggcacgttt aaactcttta aacaatcttt gaactcttcc
     8521 tcaacccgcg ttagcatggg atatctcatt actttaagct cacgcttaaa tgaatcagtt
     8581 tttacatctc cctgactgat tttgagttca atttcatcaa taatctcttt aacactccct
     8641 ttatctctgg cagtaatctc aactatttct cttacaatct cccgcgtttc attagtattc
     8701 actttgcatt ttaaaagaac cctgtttagt atttcgatcc ttaaagaact tggcacctcg
     8761 ctgagtataa ttgcctgttc tatgctgatt ttttgagaat aaaaggcata cttaatttct
     8821 ttttctaact cacagagccg catatacttt tcaaaatttt cctcgacgga agaatcccta
     8881 aaaatgggag caccctttgg gtaagttcac tctgatctaa cccgcaaagt cttttgaatt
     8941 ttaaaagaag ctctgccttt tccatttcac caagtctttc ttggttttca taaaaaacga
     9001 ttttcaaaca ctcctcattt gagatttcgt cagatttata gatctttgaa aaaactttct
     9061 ccagacctaa ttccttgcat gatttaagcc tcttgtatgc ggagattaac tggtatttat
     9121 tcccttttct cttaagacca ggggatttat tacgcctact tctttgattg aatttaatag
     9181 taaagatata tccttgcttg aacctaggcg gaatgtctca tcatcgaagt caatttttga
     9241 taggggaatg ttatctaact tcatacaact actcctcaca actaatgttt ggggaatcat
     9301 taagcagaat aaaaattaag agtataattc gttaatcgtg actcttaggt tgggaattgt
     9361 gattttcaat ccggtttcgc aatacccgtt gcacgatcca caaaaaccaa ttcacaatcc
     9421 acgattacca ttctaaaaca cggtgagaat tccgaaagcg gttcctattg tagttagagg
     9481 ggcgaggtct tcctgagtat caatagctct ttcgaccttt tttaccgcgt cttttgcatc
     9541 atagtaaagc gagtcgtcgg taacgagttt ccctatagta ccctcccctt tgtctattct
     9601 ctttagtatt gagttaagat tcgttgccgc ctctgtcgcc tgattataaa gcgtatcgtc
     9661 tttaagcaat ttcccgaatg ttccctcagc actgttaagc ctctttgcaa gttggcctag
     9721 gttcgccatc gtttctttcg tctcattata gagtgattca tcctttacaa gctttcccaa
     9781 ggttcctttt ccctgcttta tatcgctgga tatttcgtta aggttagcga gtgtatctct
     9841 ggcctcattg tagagggaat cgtcttttat tagctttcca agggttcctt caccacgagc
     9901 tgccccacta agcactgtgt ctaaactatt taatatactg cctatctttt caccattatc
     9961 tgaaaataca gactcaatcg agcttactgc tgactccacc ttagcaagaa tttcactcac
    10021 atccgccggc tcttgacttt gaagtatgct tccaggttcc gcaggcggac tttcaggaga
    10081 accaaacgta agattgatgt agcttgtacc aagaaggctt gtgagtttaa tcgtcgccag
    10141 tgaatcattc tttacaggtg ttcccttctt tactttcaag gtcacttcta tctttccgtc
    10201 tgctatcttt atctttgaaa tctttcccac ttcgacgcca gataacttca ctggattccc
    10261 ctctttaagc tcgccaacag atttgaaata ggttttaaat aaatactctt ttgtaaacaa
    10321 aggaatttct gtaataaact cgaatattat aagaagtatc actaaaccca aaacgaaaaa
    10381 gatgcctacc ttaacctctg ttttcatagt ctcctacctc cttcttctct ttcaaactgt
    10441 gtggttataa acttccttac aagcggattt gcacttctgt ctatctcctg tggagtccct
    10501 acttctataa tttgcccttc atcaagtact gcgattctat ctgcaatata ataggctaag
    10561 tcaatatcgt gtgtcactac gatttgtgta acctcgacgt gttttctaag atttagtata
    10621 gcatcaccta ttgtccgagc catcataggg tctagctccg aagtcggctc atcaacaaga
    10681 ataaggtcag ggttcatagc aagggcacgt gcgatagcta cccttttctt catacctcca
    10741 ctcagctcag agggcataat attttccttt ccctcaagac ccactatatt gagggtgtta
    10801 gaaacgattt ctctaacagc attttcatcc ttaatcacct tatgttccct taagtaaagg
    10861 gttatgtttt ctgcaaccgt gagtgaatta aaaagggctg aggattgaaa caccatagca
    10921 aaccggtgtt tctttcttac ctctggatcg ttgacatcca ttccgttaat tagaatctct
    10981 cctgaatctg gtccttcaag tccaattata tgacgaagga gtacgctttt cccagaccca
    11041 gtttttccaa tgacaactag cgtctcacta ggcttaacct ccaaattcaa gtccctaagg
    11101 acgtgattac ttccaaagga tttatttaat ttggttatct ttatcccaac aggtgcattc
    11161 agtcggtagt tagattcagc atctgacctt attatatccg aaatttctgt tcttatattc
    11221 atctgctttt ctgttacact aaaggctaat ctaaattgag caagattaaa agcctggtaa
    11281 taaaataatc aaaaaccaaa atcaatataa acgatagaac tactgccttt gtcaccgaag
    11341 ttcctatttc tctcggacct cccttagtct taagccctac gtagcagcag actgttgaaa
    11401 ttattatacc aaataccgta gccttaacta agccatttag aacgtcagaa aaatctacta
    11461 gctcagagag atttctataa tacacgctga agggaacatg aatatcagaa tttaccgatg
    11521 aaactatagc accgccaaac caccctatca catccatgta aattactaaa aggggaagag
    11581 cgataaagca tgcaatgaac ctgggcatta caagaaatcg gatgggattt atatccatgg
    11641 ttttaagcgc atctatctcc tgataaacac tcatagaacc aagttcagca gccattgccg
    11701 atcctaccct acctgcaaca agcacggaag ccataacagg acctagttct tttacgaatg
    11761 ataacccaac gatccccccg atattttctt cgattccaaa ctgagcaagg gtgggaccag
    11821 attggagcgc tagcacgccg cctataaata gcgagataag ggcagctact gggagtgtct
    11881 gatttccgat ttctagcatc tgtctaaata tcttctctcg attactaaga gcagccttgc
    11941 accaaaagat gctctccata agaagctgaa gaatctctcc agtggtatga aaaaa
//
LOCUS       DATDRL010000281         3810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_977530, whole genome shotgun sequence.
ACCESSION   DATDRL010000281 DATDRL010000000
VERSION     DATDRL010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977530"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VI935_09410"
     CDS             <1..587
                     /locus_tag="VI935_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HLE25850.1"
                     /translation="PFDTQPFLIRIDLLSEEEYYVFSDLKGFSEISKEHGEDEFIISK
                     FDIDVSSQKASTDWVSSRFTFRFEAPRHLDYYIFRIFIPVFLILTVSFITFFLNDYVK
                     RIEIAGANLLLFIAFSFSLSENYPRLGYLTFLDGIMAITFVINAFAVIYNFYLKWLET
                     HNKRGKAENIDKYMDWFYPSGFVLALLILILVFF"
     gene            743..1354
                     /locus_tag="VI935_09415"
     CDS             743..1354
                     /locus_tag="VI935_09415"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HLE25851.1"
                     /translation="MKPVVDFMKTTIVGGLLFLVPAVLIILLVKNALGFATKILKPIE
                     KLLPIEDMAGVAVEHLLAVVSILLVCFLAGLAARTRLGARISAAFERAIARKIPGFGL
                     IKSATGEVANIQSQSDICVALARIEDAWVLSFIVEKLDNGLLVVFVPSAPTPFAGSVY
                     YLTEDRVKRLDAPVLTGMKVITRLGVGSKELLQSYPNLEITSS"
     gene            1640..1831
                     /locus_tag="VI935_09420"
     CDS             1640..1831
                     /locus_tag="VI935_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLE25852.1"
                     /translation="MTKDPVAVSLEDSSLSAAATLRDYKIKWIPVVNQEQSRRIVGCI
                     SARKMTVYILKESSASSEK"
     gene            1939..2628
                     /locus_tag="VI935_09425"
     CDS             1939..2628
                     /locus_tag="VI935_09425"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HLE25853.1"
                     /translation="MRKVTHIFSRFWSTDRSLTVLLGLLFINIFILNPLFELGILRAL
                     FVPDVLFSLILVSGVMTVAKSRFITLLSVVFAIATFLVRWAKNFVPGTGLMTLDTFLS
                     IVFLGLLAVVVLLQVFREGPITMQRISGAVVVYLLLGLMWGVAYQLIELQQPGSFKFA
                     DVPAIGDTFSARLVYFSYITLTTLGYGDITAVSPIARSLAMLEAVIGQLFPAILIARL
                     VSMELYYRRLR"
     gene            3040..>3810
                     /locus_tag="VI935_09430"
     CDS             3040..>3810
                     /locus_tag="VI935_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HLE25854.1"
                     /translation="MRRFVHLNIIFISCLSVVISLFPSLEIQATDLTSLAEQVKRLKP
                     SVVNISATSVMGKGTSPSPFGGGQQDPFEEFFKRFFGDLPQREFRQRGLGSGFVLTED
                     GYIVTNNHVVEKATDIDVILEDGQKYDAKVIGKDPKTDVALLKINPKHKLPAVSLGDS
                     DKLEIGDWVIAVGNPFGLGHTVTVGIVSAKGRSLGLGAYDDFIQTDAAINPGNSGGPL
                     FNLQGQVVGVNTAIIAGGQGIGFAIPIDLAKNIINQLKD"
BASE COUNT         1043 a          761 c          866 g         1140 t
ORIGIN      
        1 tcccattcga tacccagccg ttcctgattc gcatagacct gctttctgaa gaggagtatt
       61 acgttttctc tgaccttaag ggctttagtg agattagtaa ggaacatggc gaagatgaat
      121 tcataataag taagtttgat atagatgtaa gtagtcaaaa ggcaagcacc gactgggtct
      181 cctcccgttt cacatttcgc tttgaggccc caaggcatct cgactattac atcttccgta
      241 tttttattcc tgtattcctg attttaactg tgtcttttat tacgtttttc cttaacgatt
      301 atgttaaacg tattgaaatt gccggcgcca accttttgct tttcatagcc ttcagcttca
      361 gcctttccga aaactatccg agactaggtt atttaacatt tctggatggc atcatggcca
      421 tcacctttgt tattaatgca tttgcagtta tttataactt ttatcttaag tggttagaga
      481 cgcacaataa aagaggcaaa gctgaaaaca tagataaata catggactgg ttttatccat
      541 cgggatttgt tcttgcctta ttgatactaa tcctggtttt cttctagcgg tggttcttaa
      601 agtagtaagt gcagatataa acttcaagga attccggggg aatctcgaag aagatctaat
      661 tttccataat tcttattcat tgaattaggt aacaccttaa gtatactctc ttactccaaa
      721 ataaatggaa aaggagcgtt atatgaaacc agtagttgat ttcatgaaga cgacaatcgt
      781 cggaggcttg ctattccttg taccggctgt gcttataata cttctggtca aaaacgccct
      841 cggatttgcc acaaaaatac tgaagccgat tgaaaagctt cttcctattg aagacatggc
      901 cggagtagca gtcgaacacc tacttgcagt tgtatcgatt cttttggtct gctttctcgc
      961 cggcttggct gcacggacga gattaggtgc gcgaattagc gccgcgtttg agagggccat
     1021 cgcaaggaaa attccaggtt ttgggcttat taagagcgca acaggtgaag tggccaatat
     1081 ccagtcacaa tcagatatct gtgttgcatt ggcacggatc gaagatgcat gggtgctttc
     1141 gtttatagtc gaaaagctcg acaatggtct tctagtggtg tttgttccaa gtgcccctac
     1201 gccctttgct ggttcagtct attaccttac tgaagatcgg gtaaaaaggt tagacgctcc
     1261 tgtattgacg gggatgaagg tcatcacgcg actaggcgta ggctcgaagg aattactcca
     1321 aagttatccg aatcttgaaa ttacgtcgtc ttgacatgaa tatgcaaagt gtgctattag
     1381 ggtgattggt agaatggtta gctatggttt ttaccaattc gatttattaa tagctagggc
     1441 agacccaagt gtccgcccta gccacaattt aggcaaccac agggattgtc cctacatttg
     1501 aatgcactaa tatttgatag aatcggtatt gatggattgg ggaacatgga tagaattaca
     1561 ggtggggaga tacccatctg gatgggggat gatttgagga taaaatacca aatataagga
     1621 aacacctcta gtagaattca tgactaaaga tccggttgca gtctcgctcg aagactctag
     1681 tttgtcggcg gcagccaccc taagggatta caaaattaaa tggattcccg tagttaatca
     1741 agaacagagc cggcgcatcg ttggttgtat aagtgcacga aagatgacgg tttatatatt
     1801 gaaagaaagt agtgcatcaa gtgaaaagtg agaggtgccg ccttgcctgc aatgacgact
     1861 gcgccttagc aactgccatc acagacacaa tttctcattc ggatgtaatc taattagaga
     1921 gccaatgagg gacaacgcat gagaaaagta actcacatct tctcccgatt ctggtcaacc
     1981 gatcgaagcc tgaccgttct actagggctg ctttttatca atatatttat cctcaatcca
     2041 ctgttcgaac ttggtatact ccgtgcatta tttgttcccg atgttctctt ctctctaatc
     2101 cttgtctcag gtgtaatgac tgtagccaaa agtagattta tcactttgct atctgtagtt
     2161 ttcgctatag caaccttcct cgtcagatgg gcgaagaact ttgtgcctgg tacgggttta
     2221 atgactctgg ataccttcct atctattgtg ttcttgggct tgctagcggt tgttgtgcta
     2281 ctccaggttt ttcgtgaagg gccgatcact atgcaaagaa tcagcggggc tgtcgttgtc
     2341 tatctgctgc tcgggctaat gtggggcgtg gcgtatcaac tgatcgaact gcaacaacca
     2401 gggtcgttca aatttgcgga tgtaccagct atcggcgaca cgttctcggc gaggctcgtc
     2461 tacttcagct acattacact tactacactc ggctacggtg acatcactgc agtgtctccc
     2521 attgctcgct cattggctat gctagaggcg gtgataggtc agctctttcc agccatcttg
     2581 atagccaggc ttgtgtccat ggagctttac tacaggaggt tgaggtaggt gaaatgtgtt
     2641 acaacttgtc agtggataat tgatttggtt ccaatctgag ctctgaataa gctgatactg
     2701 aaatgaagaa atctgctgca agttgctggg tatcagcaca tcaaaggaag gcttcgtcgt
     2761 ttcaatcttg atctggagac ctaaaggtct ccgctacatt ttaaatccct cacaaagcac
     2821 aaattgtagc cgaaggcttt agccttccat gaactgtatc tttagtcaat gacagtgcaa
     2881 acactgtggc acgctatagg ggattatcaa attaaacatg caaatatcag ctcatagaac
     2941 ccgatgattc cttcgcaagt agcgccttga ctataacttt tacttgactt cacacggctc
     3001 taatatatca tatttttatt atccggaggt tttcaaaaca tgagaagatt tgtacatttg
     3061 aatatcatat ttattagttg tttatcggtt gttatatcat tatttccgag cctggaaata
     3121 caggctacgg atcttacatc tttagccgaa caggttaaga ggttaaagcc atctgtcgta
     3181 aatataagtg ctacaagtgt aatgggaaaa gggacatctc catcaccatt tggaggtggt
     3241 cagcaggacc cgtttgaaga attcttcaaa agattctttg gggaccttcc tcagagggaa
     3301 ttcagacaaa gagggctcgg ttcggggttt gttttaacag aagatggata tattgtgaca
     3361 aataatcatg tagttgagaa ggccactgat attgacgtaa tccttgagga tggacaaaag
     3421 tacgatgcca aggttatagg taaagaccca aagaccgatg ttgcgctcct taagataaat
     3481 cccaaacata agctacctgc agttagcctt ggagattctg ataaactaga aatcggtgat
     3541 tgggttatag cagttggaaa tccatttgga ttaggacaca cagttactgt aggcatagtc
     3601 agtgcaaagg gtaggtcgct tggtctaggt gcttatgacg attttattca aactgacgct
     3661 gcgataaatc cagggaatag tgggggtccg ctttttaatc ttcaaggaca ggttgttggt
     3721 gttaatactg ctataattgc cggtggtcaa ggaataggat tcgcaatccc gattgatctt
     3781 gcaaagaaca taattaacca actgaaggat
//
LOCUS       DATDRL010000282         5869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_358335, whole genome shotgun sequence.
ACCESSION   DATDRL010000282 DATDRL010000000
VERSION     DATDRL010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358335"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /gene="rpsB"
                     /locus_tag="VI935_09435"
     CDS             complement(<1..399)
                     /gene="rpsB"
                     /locus_tag="VI935_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HLE25855.1"
                     /translation="MKQLLEAGVHFGHQMSHWNPRMKPYIFGSRNGIHIIDLQQTANL
                     FKMAYNFVRNVIADGGEVLFIGTKKQAQGIIAEEAIRAGMPFANTRWLGGTLTNFATI
                     RSRVDYLLELKKMEEEKQLDLLPKKEAKGLK"
     gene            1245..2114
                     /gene="sucD"
                     /locus_tag="VI935_09440"
     CDS             1245..2114
                     /gene="sucD"
                     /locus_tag="VI935_09440"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HLE25856.1"
                     /translation="MSILVNKDTRVVVQGITGSAGLFHATQCREYGTKVVGGVTPGKG
                     GTEVEGFPVFNTALDAVKETKANTSLIFVPAPFAADAIVEAVDAGINLVVCITEGIPT
                     LDMIKVKRYMEGKKARLVGPNCPGVMTPGEAKVGIMPGYIHLPGRIGVISRSGTLTYE
                     AVWQLTNLGMGQSTCVGIGGDPVLGSSFIDILKLFEKDKETDAVVLIGEIGGSAEEEA
                     ADYIKKKLSKPVVAFIAGSTAPKGKRMGHAGAIISGSSGTAKDKIMALERAGIKVSPS
                     PAEIGEVVKMVLG"
     gene            complement(2136..2303)
                     /locus_tag="VI935_09445"
     CDS             complement(2136..2303)
                     /locus_tag="VI935_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HLE25857.1"
                     /translation="MPIDEKLLEILACPKCKGGIRLKDDQSGLICDQCKLLYPIKDDI
                     PIMLIDEAIKL"
     gene            complement(2568..3518)
                     /locus_tag="VI935_09450"
     CDS             complement(2568..3518)
                     /locus_tag="VI935_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLE25858.1"
                     /translation="MNILVTGVAGFIGSHLAERLLREGFQVIGVDSFLDYYPKKIKEN
                     NLKGLVDNNRFTFIESNIFELNLKEILKKVDAIFHQAAIPGVRASWGKEFNKYVENNI
                     LGTQVLLEACKDARISKFIYASSSSVYGDADELPIKETSATKPVSPYGVSKLDGEHLA
                     VLYFKGYSIPTVSLRYFTVYGPRQRPDMGFHKFITAVLLGREIEIYGTGEQTRDFTFI
                     GDAVEANLQALMKGKNGEVYNIGGGSRITLIEVIKIIEEIAGRKAILKYVEHQRGDVQ
                     HTYADISKAKRDFGYTPRVDIYEGLKRHYDWLKENLDIYR"
     gene            3732..4724
                     /locus_tag="VI935_09455"
     CDS             3732..4724
                     /locus_tag="VI935_09455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HLE25859.1"
                     /translation="MRILVIRRGAIGDAMVTLPTLAILREHYPYAYIEVIGNHEYWKI
                     AHKRYYVDAITAGEPWLTPQLYLRDGKFGKEIADYFSSFDLILGYINGAEGIIEENLQ
                     RIGAKQILIYPPFPKGDDLHAADYTALVLREIGLEVNPPLFPKIHLSSEDLDFASQFL
                     SPFMNYEPLVAIHPRTYGIKGWSIEKFINIGKWIENTLGRKSTWLLGPAEEENLKLIK
                     SNFPSSPLLHMNSLPSVAAVLSLSDLYFGCDTGISHIAAAVGAPILALFGPTNPLVWG
                     PRGKRVWIIKADGMFKIQEDEVKQIILKYHLSQGKEEYKILTELGLETREGSEL"
     gene            4785..>5869
                     /locus_tag="VI935_09460"
     CDS             4785..>5869
                     /locus_tag="VI935_09460"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HLE25860.1"
                     /translation="MSLSPFELEIFQNVLSSIAEEMGMVLVRAGFSPNIKERRDLSCA
                     IFQANGEMIAQAAHIPVHLGSMSFAVKEVLEEPYIKEGDIFILNDPFKGGTHLPDVTC
                     IAPVFVDGKVEFFAASRAHHADMGGITPGSMPLSTSIHEEGIVIPPSKLYTKGRLNKS
                     LFQKILSATRDPEEREGDFKAQMGSLELGEKRLKAVMQKYSLEKIKKAGFELLNYSER
                     MMREVIRQIPDGVYKFEDYLDGDGAGTRKIPIRVAITISGGEAKVDFSGSSPQVNGCI
                     NTPLSVTTAAVLYVFQCFAPPEMPLNSGPLRAIKIHAERGSILNANYPAAVAGGNVET
                     SQRVVDVVFGALAKAIPEKIGAASSGS"
BASE COUNT         1836 a         1178 c         1124 g         1731 t
ORIGIN      
        1 tttaagtccc tttgcctcct tcttaggaag aaggtccaat tgtttttctt cctccatctt
       61 cttcaactca agcagatagt caacccttga gcgaattgta gcaaaattgg tgagagttcc
      121 acctaaccaa cgagtattag cgaatggcat tccagccctt attgcttctt cggcaatgat
      181 cccctgagcc tgtttttttg ttcctataaa taggacctca ccaccgtcag ctattacgtt
      241 cctaacaaag ttataagcca ttttaaatag gttcgcggtt tgctgaaggt ctattatgtg
      301 tatcccgttt ctactcccaa atatgtaggg tttcatccta gggttccagt ggctcatctg
      361 atgcccaaag tgtacacccg cttcaagaag ctgtttcata ttgattacgt cgcccacatt
      421 gttttcttcc acaattaact cctccggtat tgaagttaga aatataaatt agccaataac
      481 ctttaacata aaaaaccttt tttgcaagtt tattaactat taaatatcat gatactgaat
      541 tatatatact taaatagcag gatagtacct aagtcaggat aatcctattg aaattgcata
      601 acttaccatt ctatttatcg tatttaaata caccttatgc taagggcaac ctgcattctc
      661 accccagtaa ttataccata gggatatgaa caggtattgt ttaaaattca tcccatctta
      721 ttatcgcttt gcactacatt gaatacacaa actaattagt actctaggac cgctgcctct
      781 tgatacatca ctttactttc tatgacttga tttaggacga aaaaaagatt tgttaatacc
      841 agaactaagc aaaacaacgg taagttgaag tctactccta acgcctagtt ttttaaagat
      901 ttttactaag aacaattcca tttttatgaa tcatggtttt gaataaagtg ctttaatggt
      961 agaatattcc taaaagaaaa taggcctgat gaaatactag aatataaaca atcaattaag
     1021 ctgttcaaat actatgtctc gtcggtaata atacctatca tttaaaagaa tatttttata
     1081 cctgagaaaa cccttaataa tgtaatttaa aaggaaatct ttcatgattt tgttattaga
     1141 gtttttcgat gtgccacaaa agctgatcag ttgaaacacc gccctagcta tgatatgatt
     1201 ccaatccgat ttacctcaca attcaccaaa ggagtagaga agtaatgagc attctcgtaa
     1261 ataaggacac acgcgttgtg gtgcaaggta ttactgggag cgccggactt ttccatgcta
     1321 cccaatgccg tgaatatggg actaaagttg tgggtggagt tacacccgga aaaggtggta
     1381 ctgaagtaga aggtttccct gtgtttaaca ctgcattaga tgcagtgaaa gaaacgaagg
     1441 caaatacctc actaatattt gtccctgcgc cctttgctgc agatgctata gtagaagccg
     1501 tagatgcagg aatcaacctc gttgtgtgca taacggaagg aattcctacc cttgatatga
     1561 ttaaggttaa aagatatatg gaagggaaaa aagcaagact tgtcggtcct aactgtccag
     1621 gagttatgac ccccggcgaa gcaaaagtgg ggataatgcc aggatacatt caccttcccg
     1681 gaaggattgg agtaatatca agaagcggaa cactcacata tgaagctgtc tggcaactga
     1741 cgaatttagg tatgggacag tctacatgtg ttggaatagg tggagatcca gttcttggct
     1801 cttccttcat tgatattcta aaactctttg agaaagataa agaaactgat gctgtggttt
     1861 taatagggga aattggagga agcgcagaag aagaagcagc ggattacatt aagaaaaaac
     1921 tcagtaaacc tgttgtggct ttcatagctg gttctaccgc acccaaagga aagcgaatgg
     1981 gtcatgcagg agctattatc tcaggtagtt ctggcactgc aaaggataag attatggccc
     2041 tagaacgtgc aggtataaaa gtatcaccaa gccccgcaga gataggagag gtcgtaaaga
     2101 tggtgcttgg ctgaagtgat ctatgatgct gtcttctaaa gcttaattgc ttcatcaata
     2161 agcattattg gaatatcatc ttttatggga tatagaagtt tacattggtc acatattaaa
     2221 cctgattgat catctttaag cctaataccc cctttacatt tcgggcaggc aagaatttca
     2281 agaagctttt catcaatagg catattaacc tccatttaaa atacttatac cgttcctacc
     2341 agaaattagt gcgttgaaaa gagtagtcat cgttagcgaa gcgacctagt aaggggtgtc
     2401 attccgaatg tagtgaggaa tctcaattag attgctttgt cgatgtcttt tatttccatt
     2461 tctcgcacac tgcgctcgaa ataatcctgc tcgcaatgac agccttcttc aagtactata
     2521 aaaaaatcaa aataatttac tttcactagc ctagcttggc taaaattcta tctgtaaata
     2581 tccaaatttt cttttagcca atcgtaatgt ctttttagcc cttcataaat atcaacccta
     2641 ggagtataac caaagtctct ttttgccttt gatatatcag cgtacgtatg ttgtacatct
     2701 cccctctgat gctctacata ttttaagatt gcttttctcc ctgctatctc ctcgatgatt
     2761 tttataactt caataagtgt tattctgctt cctccgccaa tattgtatac ttcgccattc
     2821 ttccctttca tcaaagcctg cagattcgct tctaccgcat cgccaataaa ggtaaagtct
     2881 cttgtctgtt cacccgtgcc gtagatttct atctctcttc ctagaagaac tgcagttata
     2941 aatttatgaa accccatatc agggcgttgc cttggtccat agactgtaaa gtatctcagg
     3001 gacacagtcg gaatactata ccccttaaag taaagaactg ctaaatgctc cccatcaagt
     3061 tttgaaaccc cgtaaggaga gacaggtttt gtagcagaag tctctttaat aggaagttca
     3121 tcagcgtctc cataaactga agaggatgat gcatagatga atttgctaat tcttgcatcc
     3181 ttacaagctt caagaagaac ctgggttccg agtatattgt tttctacata tttattaaac
     3241 tcctttcccc aacttgctct aacacctgga atggcagcct gatgaaagat agcatcaacc
     3301 ttttttaaaa tctcctttag attcaactca aatatattgc tctcaataaa tgtaaaccta
     3361 ttattatcaa ctaacccctt taaattattc tccttgattt ttttaggata ataatcgaga
     3421 aatgaatcta caccaattac ttgaaaacct tctctaagaa gcctttctgc aagatgagaa
     3481 ccaatgaaac cagccacgcc tgtaaccaat atattcataa gcttttttca tcctttatat
     3541 tttatgtttc ataatttgct tatatccgat aagcctagta gctaaacaaa gccgcaaacg
     3601 taataaacaa cgtcaatact taatgttaga cttagaatca tatcacataa cacagattct
     3661 tttaaacact actcgtattt atgtataatt taaacaagga aattttaaaa agttcttaga
     3721 gggcatatcc aatgcgaatc ttggtgatta gacgtggagc aataggagat gctatggtaa
     3781 cacttcctac actagctatc ttgagagaac actatcctta tgcttatata gaggttatcg
     3841 gcaaccatga atactggaag atcgcgcaca agagatatta cgtagatgct attacagcgg
     3901 gagaaccctg gcttactcct cagctttatt tgagggatgg taaattcggt aaagagattg
     3961 ctgattattt ctcttctttt gatttaattc ttggatatat aaatggtgct gaaggaatca
     4021 tcgaagaaaa cctacaaaga atcggggcta aacagattct tatctaccca ccctttccta
     4081 agggagatga cctacacgca gccgattaca cagctctagt tcttcgagaa ataggtctcg
     4141 aagttaaccc tccattattt ccaaagattc acctaagcag tgaagacctc gattttgcct
     4201 ctcaatttct atcgcctttt atgaattatg aaccattagt agctatacat ccaaggacct
     4261 atggtataaa ggggtggtca atagagaaat tcattaatat tgggaaatgg attgaaaaca
     4321 cactaggcag aaaatcaacc tggctattag gtccggcaga ggaagagaat ttaaagttaa
     4381 tcaaatctaa tttcccctcc tcccctcttc ttcacatgaa ttcacttcca agtgtcgccg
     4441 ctgttctcag tttatctgac ctctactttg gatgtgacac agggatttct catattgccg
     4501 cagcagtggg agcaccaatt cttgcgctct ttggacctac aaatcccctt gtctggggtc
     4561 caagaggaaa aagggtatgg atcataaaag cggatgggat gtttaaaatt caagaggacg
     4621 aagtaaagca gattattctt aaataccatt tgagtcaagg caaagaagaa tataaaatcc
     4681 ttaccgaact cggactagaa acaagagaag gatccgaatt atagccgaat tgttgaaatg
     4741 gatattaact ttttcgtttc ttacaataca aaagggtact ccgaatgtcg ctaagcccat
     4801 ttgagcttga aatattccaa aacgtactaa gctccatcgc agaagagatg ggaatggtac
     4861 ttgtgcgtgc gggattttct ccaaacatca aggagcgaag ggatctctca tgtgcaatat
     4921 tccaggcaaa tggagaaatg atcgctcaag ccgcgcatat tccggttcat ctaggctcaa
     4981 tgagttttgc agtaaaagaa gttttagaag agccttacat taaggaagga gacatattta
     5041 ttttgaacga cccgtttaaa ggaggaacac accttcccga tgtaacatgt atcgcaccgg
     5101 ttttcgtaga tggcaaagtt gaattttttg ccgcatcgag ggcgcatcat gcagatatgg
     5161 gtggaataac accaggctcg atgcctcttt ccacctccat tcatgaggaa ggaatagtta
     5221 ttcctccctc caagctgtat acaaaaggaa ggctaaacaa gtctctcttc cagaaaatac
     5281 tctctgccac ccgagacccg gaggaaagag aaggagattt taaggctcaa atggggtcgc
     5341 tagagctggg agaaaagaga ctaaaagcag ttatgcaaaa atattcgcta gagaagataa
     5401 agaaagctgg ttttgagctt ttaaactaca gcgaaagaat gatgagggaa gtgataagac
     5461 aaataccgga cggagtttat aaatttgagg actacttaga tggtgatgga gctggtacta
     5521 gaaaaatacc gattcgagtc gctataacta tatcaggagg agaagcgaaa gtagatttca
     5581 gtggaagctc tcctcaggta aatggttgta taaatacacc gctaagtgtt acgacagccg
     5641 ctgtattata tgtctttcaa tgcttcgccc ctcctgagat gcctttaaat tcgggcccac
     5701 ttagagcaat caaaatccat gccgaaagag gctcgatact taacgccaat tatcctgctg
     5761 cagttgcagg aggaaacgtt gaaacttcac aacgtgttgt agatgtagtt tttggagcgc
     5821 ttgcaaaagc aattccagaa aaaatagggg cagcaagctc aggctccat
//
LOCUS       DATDRL010000283        24781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1947213, whole genome shotgun sequence.
ACCESSION   DATDRL010000283 DATDRL010000000
VERSION     DATDRL010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 24781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24781
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947213"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VI935_09465"
     CDS             <1..461
                     /locus_tag="VI935_09465"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HLE25861.1"
                     /translation="IELLIAKRNERILKAQGGIEFDKNGYRVIVVMHVAFFVSLISEK
                     VFLQRTLNTYWFVFAVLFVGAQFLRYWAIKSLGVYWNTKILVLPNHQLINRGPYKYLR
                     HPNYIAVIVEIAVIPLLFSCYITAIVVSVINLLLLNRRVRIEEMVLYRIW"
     gene            complement(566..4048)
                     /locus_tag="VI935_09470"
     CDS             complement(566..4048)
                     /locus_tag="VI935_09470"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HLE25862.1"
                     /translation="MNRRTYKKRISVKPFRRVLVANRGEIAIRVFRACTELGITTIAI
                     YSEEDAFSLHRNKADEAYLIGKGKGPVEAYLDIEGIIKLALDKKVDAIHPGYGFLSEN
                     PEFSRACKEAGIVFIGPTPEIISLMGDKAEARRFARSAGVPIVPGTEGFIKSDKEATE
                     FAELHGYPVLVKAAHGGGGRGIRIATDLKSLLENLVQARSEAKSAFGSDALILEKYVQ
                     NPKHIEVQTLGDRYGTVIHLFERDCSVQRRHQKVTELAPSITLDHGLKEKIYNAALDI
                     ARAVNYTSAGTVEFLVDREGNFYFMEMNTRIQVEHTVTELITGVDLVQAQIRIAEGYR
                     LSDREIGITKQSQIKCHGYALQCRITTEDPTNNFRPDIGRLIVYRSPGGFGIRLDAAS
                     AYVGAVITPYYDSMLVKITSWGLTFEQCISKMDRALREFRIRGVKNNIPFLLNVIRHP
                     VFRMGACDTFFLEEHPELFEIRESRDRASRLLNFIGDISVNGALARPSTWVDRPPLTP
                     PLPETDGNPKQVPEHRRILETKGPKGLADWVLKQKRLLITDTTFRDAHQSLLTTRMRT
                     YDMLRIARATSHFENDVFSFEMWGGATFDVCLRFLKECPWERLTRLRKVMPYAMFQML
                     IRGANAVGYTNYPDNVVHEFVKEAAASGIDIFRIFDCFNWIPSMKVAIETALDTGKVV
                     ETAICYTGDITDPRRDKYSLSYYVGLAKELANIGAHFIAIKDMAGLCKPYAAEKLVRA
                     IKEETGLPLHFHTHATSGNGEATVLKAAEAGADIVDLAISALSGLTSQPSLNAIVAAL
                     EGGERDTGLDVQGLHKLSAYWEVVREYYAPFEGDMKSTNADVYLNEIPGGQYTNLRAQ
                     ARELGLGERWEEVKHMFSEVNKLFGDIIKVTPSSKVVGDLALYLIQNSLTTEDVIKRA
                     DQLSFPESVIDMMRGNLGQPTGGFPLEVQRAILKSEEPIKVRPGELIPPANFDATAKR
                     LEKQFGHSISNQDVISAFLYPRVFEEFDQHRTTYGDISLIPTPAFFYGMQPGDEITVE
                     IEEGKTLLIKLFAIGEIEADGRRPLLFELNGQPRPVRIPDKSAPTVVRIRPKADLANP
                     EEVGAPLSGKLVRFFVKEGDKVNLDQPLLVIEAMKMQTNIKASEDGIVSKIFASEGES
                     VEAGDLILRLTSQS"
     gene            4417..5085
                     /locus_tag="VI935_09475"
     CDS             4417..5085
                     /locus_tag="VI935_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25863.1"
                     /translation="MIEKRFSKGEAIKFGWSMVKSNLIFFVGLFIADGLIYQALEIIS
                     KSTKENIPVLSFAFDIVYLVIQIIVQMGLIKISLRICDNMRCELADFFSDFLSCLPLF
                     FKYLSGMILYLLIVLIGIILLIIPGVIWSVKYSFVGYFIVDKGLGPIEALKESSLITN
                     GAKQDLVLFWLLISVVNLLGVLAFFIGLFVTIPITVVAIGFVYRKLLNKTEAPHITAL
                     LISP"
     gene            5270..6253
                     /gene="hemB"
                     /locus_tag="VI935_09480"
     CDS             5270..6253
                     /gene="hemB"
                     /locus_tag="VI935_09480"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HLE25864.1"
                     /translation="MLFPSYRPRRLRKTKNIREIIQETNLSVSDFLYPMFIIPGNGVK
                     EEITSMPGIFRYSIDNAIKEIKELKELGIQAVLLFGVPERKDEVGSEGYSESGIIQKA
                     LRRIKEEINDILLITDVCMCEYTSHGHCGIVKDGEVENDETLQYLGRIALSHAKAGAD
                     IVAPSDMMDGRVGVIREVLDENGFQNVAIMSYSAKYASAFYGPFREAAHSTPQFGDRH
                     GYQMDPPNIREALREISLDIQEGADIVMIKPALSYLDVIKAAKEEFDYPIAAYSVSGE
                     YSMVKAAGMLGWIDETKLIMEILISIKRAGADIIITYFAKEAARILNSSKR"
     gene            6514..6705
                     /locus_tag="VI935_09485"
     CDS             6514..6705
                     /locus_tag="VI935_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HLE25865.1"
                     /translation="MKNTLYKVVEVTTVTDEELESVINEWVQKGWSLDTIQFAMREAS
                     KRPAMAFVIFTKSNDSEEG"
     gene            complement(6736..7179)
                     /locus_tag="VI935_09490"
     CDS             complement(6736..7179)
                     /locus_tag="VI935_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLE25866.1"
                     /translation="MKATALALFVASIWGITPILEKLSLVKGSPFTVMTVRFIFTATF
                     VVIISLVSGKYKDIGNLDGKTILWTCLAGLLGGIVGLFIYFVALKQDLTSRIAPIAAT
                     FPLFTALYAFIFLHESISIQRITGIVLIVLGLILVNWDTVSGPVE"
     gene            complement(7329..8045)
                     /locus_tag="VI935_09495"
     CDS             complement(7329..8045)
                     /locus_tag="VI935_09495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HLE25867.1"
                     /translation="MKGNNRIKAVFFDAADTLFRIKGGVGNMYWSVARKYGANSTPEQ
                     IEKAFIKAFKSAPPLAFADVSTDDRKVYEKKWWYEVVRKVFSEVGMFVKFDDYFDELF
                     ETFRSKAWELFPETKDVLTVLKGKGLILGIISNFDSRIYDVCIDLGIIEYFDSFVISS
                     EVGSAKPSPEIFSIALERNEVHPFHCIHIGDSLDYDFYGARSAGIRALLIDKGGRCKD
                     RGDVDRVENLNQIISIIDND"
     gene            8253..8639
                     /locus_tag="VI935_09500"
     CDS             8253..8639
                     /locus_tag="VI935_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLE25868.1"
                     /translation="MGGTNQRTKIIAVLDDLFFSSKIKEATSNLELDLKFIKNTNGLI
                     QKLKFEKPSLIIFDLNSKVCNPLETIKELKSSPDLKDIPILGYLSHVQTDLQNEALKA
                     GCDHVLPRSKFSKDLREILAKYSTSH"
     gene            complement(9392..13144)
                     /gene="metH"
                     /locus_tag="VI935_09505"
     CDS             complement(9392..13144)
                     /gene="metH"
                     /locus_tag="VI935_09505"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HLE25869.1"
                     /translation="MLRRTESERFQLLRELLAERILFLDGAMGTMIQGHKLGEAEFRG
                     VRFKNHWKELKGDNDLLNLTCPQIIAVIHRQYLEAGADIIETNTFNSTRVSQADYGLE
                     DIVYELNRDGARIAREVADGFMALNPERKCFVAGAIGPTNRTASMSPDINRPSFRSIT
                     FQELVDAYYEQVKGLVDGGVDILLPETVFDTLNLKAAIFAIEKFFDEYPFRLPIMLSV
                     TVTDASGRTLSGQTIEAFWNSVMHAKPLSVGINCALGAKEMRPHIEELSRIAECYISC
                     YPNAGLPSPLSETGYDETPDITSSLLEDFARSGFLNIVGGCCGTTPEHINAIANRLRV
                     ISPRKIPKVTSATRLSGLEPLKIEGDKSPFIMIGERTNVTGSPQFAKLIKADDFDGAL
                     TIARQQVENGANIIDVNFDEALLDSKACMARFLNLIGSEPDIARVPIMIDSSKWSVIE
                     AGLRSVQGKSIVNSISLKEGEEKFLEQARLIMRYGAAVIVMAFDEHGQAANKADKVRI
                     CNRAYKLLTEKVGMDPTDIIFDPNILTVATGIEEHDNYAVDFIEGVREIKATCRGART
                     SGGVSNISFSFRGNNTVREAMHSAFLYHAINSGLDMAIVNAGMLNVYEEIDKELLELV
                     EDVLLNRGPDATERLIEYAERVKGMGKAKVKDESLWRRSTVNERLIHALINGIMDYIE
                     EDTEEARLQYATPLDVIEGPLMDGMKVVGDLFGAGKMFLPQVVKSARVMKKAVAHLEP
                     FMETEKGKIQNARNQGKFVIATVRGDVHDIGKNIVAVVLACNNYEVIDLGVMVPCEKI
                     LNTAREINADIIGLSGLITPSLDEMIHNATEMERQGFKIPLLIGGATTSRTHTAIKIA
                     LHYGGVVEHVQDASLIVGVCNNLLNPEKIDRYVAELRVEQEKQRQQYAAGKKKRNFVP
                     IKEARANKFKTDWKNIQIDSPDKFGLQVFQDIPLEEIVPYIDWSPLFWAWELKGVYPK
                     IFEHKDYGVQARELYQDAQKLLKEIITKKRFRARAVLALWPANSVGDDIEVYTDETRK
                     EVLGTFHFIRQQKVKDENGIYYCLADFVAPKESGHKDYIGGFAVTAGEEVEDFAKTLK
                     AKNDDYSAIMVKALGDRFAEALAEMMHKEARIIWGFGKDENLSFEDLINEKYRGIRPA
                     PGYPACPDHTEKSILWRVLDAEKNTGITLTENFAMYPPSSVSGLYFANPEAKYFHVGQ
                     IDRDQVEDYARRKNMLVEVVEKWLQPNLGYERGDFVKSDKKTTIPK"
     gene            complement(13171..13344)
                     /locus_tag="VI935_09510"
     CDS             complement(13171..13344)
                     /locus_tag="VI935_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25870.1"
                     /translation="MTKIDTAKRFLRKIDALMLDLTTEVDIDGLPEEAGKAIVMITNN
                     VKLALEILESEED"
     gene            complement(13414..13689)
                     /locus_tag="VI935_09515"
     CDS             complement(13414..13689)
                     /locus_tag="VI935_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25871.1"
                     /translation="MKQDDLMLFIGVPIEVAIMDSRIVLKGIIEAVNNEFAKFSEIED
                     LFVLVHGRRLSIKLMLSGSFIDLEALLKNLQFPCSLFDECRRLIRSA"
     gene            13864..14301
                     /locus_tag="VI935_09520"
     CDS             13864..14301
                     /locus_tag="VI935_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25872.1"
                     /translation="MIKKVIKRHAKVKERQNLSLFTLVLVFLVILITDCNKKENVIPA
                     AHIPGIRASITKILISPLAFDGAIVAVEGIAHDVKQEKSDGKDKTSTIFKLSDLRGNY
                     INVSTPGKREIFEYDFLVVGGIYRRAENLIEAQQIEIVLLEEK"
     gene            complement(14777..15604)
                     /locus_tag="VI935_09525"
                     /pseudo
     CDS             complement(14777..15604)
                     /locus_tag="VI935_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
     gene            16029..16688
                     /locus_tag="VI935_09530"
     CDS             16029..16688
                     /locus_tag="VI935_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase partner protein"
                     /protein_id="HLE25873.1"
                     /translation="MADQKTTPPNNNFSEQHSKTESIQPQNDTHIPPPAVLHPRLVLD
                     TSLFVNPDAQRQFGENVNQAVNRFLKITRENNIEIYMPLSVFRELSNFTSPEALITFR
                     RDAIVRAPDLYNLQVPAAIFHIFIGELRNRINKGLRITEEAIHKEPTAENIRRLRQEY
                     REALRSGMVDSVEDLDVVLLAKEIGGAVLSADEGIANMAEALGIEVLSATYFIHRYGA
                     E"
     gene            16707..17795
                     /locus_tag="VI935_09535"
     CDS             16707..17795
                     /locus_tag="VI935_09535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase"
                     /protein_id="HLE25874.1"
                     /translation="MPDVERGVEEREYNGLKYFRYADDIGTTLRGTVVLFDTSGQRPI
                     RTIPGFPHIKRVYRLRPGVQRFFHTNPFFVEEKLDGYNVRLLLHQGQLLALTRGGFVC
                     PFTSEWAEIWWHRYNLERFFTTYPDYVLFGEVLGDNPYNSQRDPSIPPGLYFFTFDIM
                     RSDGEFLRVHDRYELVEGYELPTVPLLGQFTSDRIDELFGILRDLNEKEREGVVLKTN
                     TSKKSLKFVTPLTDLRDIRDNLIIEFDLEPGFITNRLLRISLFVREFGLNEEEYASQV
                     GKAFLDGYSTLETFESSNETYNIYVKLPKTWNSLRTLLSGYVPIKTDRVEPVILDGLE
                     MLRVEFRRVFRKSTRRYREILRGYGHID"
     gene            17981..18691
                     /locus_tag="VI935_09540"
     CDS             17981..18691
                     /locus_tag="VI935_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLE25875.1"
                     /translation="MILKDKVAIITGGTGALGRAVVSAFLKEGAKVACTYIVDEELKG
                     VSHFIESYKSQIKFIKANVTKEEEVKKTIQETLEEFGKIDILVNIVGGFAYAKILDTD
                     EKTWDSMMNMNLKSAYLCSKAVLPHMIKQNYGKIVNISSRPALKGSAGVGAYSASKAG
                     VLNLTETIADEVKDYEINVNTILPSTIDTPANRRDMPNADFSKWVKPEEIARVIVFLA
                     SDDSKPISGAAIPVYGKA"
     gene            complement(18707..20218)
                     /locus_tag="VI935_09545"
     CDS             complement(18707..20218)
                     /locus_tag="VI935_09545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLE25876.1"
                     /translation="MTENSIYSQTDASEIQIARLTVLADDRYLDAVADLIADIAKVTG
                     LDEESARKLDAIINEVLQKIFEYGYEGDTTQSVNVIVSRRTHSLVVTIKDKGLPFDYL
                     RIEQGEDPRFSSFLSKGYADQVHFINLGPAGNHVELFKDLPSTDIRYQSDPPEHREAL
                     DAVEAHPEEALIMRIMQPDEAVELARLVYRCYGYTYPSEFVYYPEQVEAKQRSGLMKS
                     FAVYNSRGEMVGHIALTFDHPGAKVAESGQAVVDPRYRGHKIFERIKHFLLDYAVANQ
                     LIGIYGEAVTVHPYSQKGNLALGAHEVGFLLGYSPGTVSFRTITDQKRPRRQSIALMY
                     TSILKSATAPIFPPGAYRTILKQIYDILGIEREIVDVKADRTDERPRQTGRIKVSMRP
                     DHNQALLSVEAIGQKTFDEIRFHLKQLCLNRFECIYVDLPLSNDLTGVLGTQLRKIGF
                     FYGGVIPELRDVDILRLQYLNNVVITPEDIAVASDFGRTLLDFILQDKSDVTA"
     gene            20579..20794
                     /locus_tag="VI935_09550"
     CDS             20579..20794
                     /locus_tag="VI935_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25877.1"
                     /translation="MIRPSHRLSILLFVTLLLSSTVTLTKEPENLHLAKQAAIFYHDS
                     GEYDYDLGVVVQEAQTYLEERIPQKRS"
     gene            complement(20855..21949)
                     /locus_tag="VI935_09555"
     CDS             complement(20855..21949)
                     /locus_tag="VI935_09555"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLE25878.1"
                     /translation="MKKLIEFIRPQQKLHTVIFFVTSRCNALCRTCFYWQELNQKGDL
                     SFDEIKILSETMSHFHDLLLSGGEPSLRNELPEIVNLFYRNNGIRRIYFPTNGLKPQR
                     IRELVGRILQENNELEVYLNNSIDGLPETNDRIRSVPGNFNKSLETIRFTQTLKPRFG
                     PRLQINVNSVICAENYNELVELGRFFLDNERLDYHYFQIIRGDPLDKSLKKVPTEELR
                     SLYKEITKIQKVYASRQGDNGGAVNGWFRKSIYASVFNFHNRTQFNNYTENTPWPMPC
                     TAGETSLVIDYDGTVRACELRGSIGSLRDHGCNFQALWNSKVRDEEVKSIAKDKCFCT
                     HVCFIHDSMWFSPRVKFWELPKSYITRYSW"
     gene            22196..22987
                     /locus_tag="VI935_09560"
     CDS             22196..22987
                     /locus_tag="VI935_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HLE25879.1"
                     /translation="MEQMFLAIFIDLNNIPSKIKLERLMNALILSEQSNYNFIFAVKS
                     AYGVLHSVSQEFRTELRDYNFSILETPRIGLKKNRADLFISIDAFETLYVNNPGIDRY
                     IFVTSDSDFTVIMDKLRKYGKEVWLVCRKADETRQILAKSCDKMLFIEDFYQFEDEKS
                     HKEYIEANDNKAKELFKEVLRNIDSERLPFNISVINDRMKQLDRSFDVRHTSYKKFIE
                     LIRAFHTLGIITIEESESGVVRITDIDLSKIEVGNSGSNLTNDLS"
     gene            complement(23152..24693)
                     /locus_tag="VI935_09565"
     CDS             complement(23152..24693)
                     /locus_tag="VI935_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HLE25880.1"
                     /translation="MITWLENSPILFTSLISFFILVVLLGFGWIVRTRLKIRLGLSYV
                     LFSIFVAIGLWLTLNHKYGLLKEVKLHTFIKWYTALVVFFAVWFLIKIFSVFFWDYYL
                     FQYRKTHVPGLLRSVVTVLILIIAILVIVRFVFDKQLSGLLVASSVTAGLLAFALQDF
                     LGGIIAGIALNIEPPFQVGDWVQVGDKDGEVVDINWRATTIRGRDFNYRVIPNSIISK
                     EEITNFYRPTRLHALLLYVGVEYAALPNLVKEILINCALETRGVVSTPRPLVRLVNYG
                     DFAITYQLKYFIEDRPISEDIQSDVMIRIWYTLRRNNIRIPFPIRDVFIHEEVGKEQA
                     IEEMNRRRVRIEELLRRVSIFEPLKDTEIETIASTGRLWHFGRGEHIVKQGEAGSSLF
                     LVLGGSAAVDIKIPDDGRIVTVAHLNTGDFFGEKSLLTGELRSASVVAETDLEVVEVE
                     KANILPILENNPGIMEDLSKRLAERQLINEGFFKEGKKAEEVAVMRINYTERFLQSMK
                     TFFGL"
BASE COUNT         7624 a         5109 c         4586 g         7462 t
ORIGIN      
        1 tgatcgaact tctcattgca aagcgaaacg aaaggattct taaagctcaa gggggaattg
       61 agtttgataa aaatggctac agagtaattg tagtaatgca tgttgctttt tttgtttcgc
      121 ttatatctga aaaggttttt ctccagagaa ctttaaatac atattggttt gtttttgctg
      181 ttctctttgt aggggcacag tttttacgct actgggcaat taaaagtcta ggtgtatact
      241 ggaacacaaa aattttagtc cttccaaatc atcagttaat taacagaggc ccctataaat
      301 accttcggca tccaaattac attgccgtaa ttgtagaaat tgccgtaata ccattgcttt
      361 tttcatgcta tataactgct atagttgtta gtgtcattaa cctactttta cttaatagaa
      421 gggttcgtat agaagaaatg gtgttgtata ggatttggta aagggtgggt tttattttag
      481 cttgcgatca actcaagtat tcaaacaaag aaaaggcagt atctttatta ctcttttata
      541 ggatcgaaaa aataatgaaa aaaatttagc tctgggacgt aagacgaagt attaggtcac
      601 ctgcctcaac actttctcct tcagatgcga agatcttcga tactatgcca tcctctgagg
      661 cctttatatt agtctgcatc ttcattgcct ctattactag aagtggttga tcaaggttaa
      721 ccttatcacc ttctttaacg aagaacctta caagcttacc gctaagaggc gctcctacct
      781 cctcagggtt tgctaaatca gctttcggcc ttatcctgac cacagttggt gcagatttat
      841 cagggattct tacagggcgt ggttgcccat ttagttcgaa taaaagtggc ctccggccat
      901 ctgcttcaat ctcaccgata gcaaatagtt ttataagtaa ggttttaccc tcttcaattt
      961 caacagttat ctcgtccccg ggttgcatgc cgtagaagaa ggctggtgtt ggtataagag
     1021 aaatatctcc gtaagtcgtg cgatgttgat caaactcctc aaaaacacgg ggataaagaa
     1081 atgcagatat aacgtcttgg ttacttattg agtgaccaaa ctgcttttct aatcgcttgg
     1141 ctgtagcatc aaagttggct ggaggaataa gctcacctgg acgcacctta ataggctctt
     1201 cgcttttgag gatagccctt tgtacttcta gtggaaaccc gccggtaggc tgacctaagt
     1261 tgccgcgcat catatcaata acagattctg gaaagctcaa ttgatctgct ctttttatta
     1321 catcctcagt ggtgaggcta ttttgaatca aatatagtgc aaggtcacca actaccttag
     1381 aggatggtgt aaccttgata atatcaccaa agagtttatt tacctctgaa aacatatgct
     1441 ttacctcttc ccatcgctcc ccaaggccaa gctcgcgtgc ctgagccctt aagttagtgt
     1501 attgtcctcc agggatctca ttcaagtaaa catcagcgtt tgttgatttc atatcgcctt
     1561 caaagggggc ataatactcc ctaacaacct cccaataagc agatagttta tgtaaacctt
     1621 gcacatcaag tccggtgtca cgttcccccc cttccaaagc agctacaata gcgtttaggc
     1681 ttggttggga tgtaagtcca gacaaggcac ttatagccaa atcaactata tctgcaccag
     1741 cttctgctgc ctttagcaca gtagcttctc cgttaccact tgtagcatgg gtatgaaagt
     1801 gaagtggcaa tccagtttcc tcttttatag cccgtactag tttctcagcg gcataaggtt
     1861 tgcagagacc tgccatgtcc ttgattgcaa taaaatgagc gcctatgttt gcaagctctt
     1921 ttgctaagcc cacatagtaa ctaagagaat atttgtcacg ccttggatca gtaatgtcac
     1981 cagtgtaaca aatagcagtc tcaaccacct taccagtatc aagagcagtc tcgatcgcca
     2041 ccttcatact tggaatccag ttaaaacaat caaagattct gaagatgtct attccagaag
     2101 cggcagcctc ttttacaaat tcatgcacta cattgtcagg gtaatttgta tatcctacag
     2161 cgtttgctcc tcgaataagc atctgaaaca ttgcatatgg cataaccttt ctaagacgtg
     2221 taagacgttc ccatggacac tccttaagaa aacgtaagca gacatcaaat gtagcccccc
     2281 cccacatttc aaaagaaaat acgtcattct caaaatgaga tgtagcccta gcgatacgta
     2341 gcatgtcata ggttctcata cgtgtagtaa ggagtgattg atgcgcatct cgaaatgtag
     2401 tatcagttat aaggagacgt ttttgcttaa gaacccagtc agctaaaccc ttaggtccct
     2461 tagtttcaag aatacggcga tgttcaggaa cttgttttgg attaccatct gtttcaggaa
     2521 ggggcggtgt tagaggtggt ctatctaccc aggtcgaagg ccttgccaga gccccattta
     2581 cacttatatc cccgataaag tttagaagcc ttgatgctct atccctagac tcccgaatct
     2641 caaaaagctc tggatgctct tcgaggaaaa aagtatcaca agcacccata cgaaaaacgg
     2701 gatgccgaat gacatttaga agaaagggga tattgttttt cacacctcgt atcctaaact
     2761 cccttaatgc gcggtccatt ttacttatgc actgttcaaa cgtaagcccc caagacgtga
     2821 tcttaaccaa cattgagtca tagtatggcg ttattactgc accaacgtag gcactagccg
     2881 cgtcgagtct tataccgaat cctccgggtg agcggtagac gatgaggcgt cctatatcgg
     2941 gtcgaaaatt atttgttggg tcctctgtgg ttatacgaca ctgaagcgca tagccatgac
     3001 actttatttg agattgcttg gtaattccga tctcacgatc agatagtcta tagccctcag
     3061 cgatacgtat ttgagcttga actagatcca ctcccgtaat aagttcggta actgtatgtt
     3121 caacctgtat acgtgtattc atttccataa agtagaaatt tccttctcta tctacaagaa
     3181 attccacggt acccgcgctc gtataattta cagcgcgagc aatgtctaaa gccgcattgt
     3241 agattttctc ttttaatcca tgatcaaggg taatagacgg agcaagttct gtaaccttct
     3301 gatgcctcct ttgtacagaa cagtcacgtt caaacaagtg aatgacagta ccatatcggt
     3361 cacctaaagt ttgcacctcg atgtgttttg gattctgaac atatttctca agtattaagg
     3421 catcgcttcc aaaggcgctt ttggcctcgg agcgcgcctg tacgagattc tcgagaagag
     3481 acttaagatc tgtagcaata cgaatcccac gcccacctcc accgtgtgca gcctttacaa
     3541 gaaccggata gccatggagt tctgcaaact cggtagcctc cttatcactc tttataaatc
     3601 cttctgtgcc tggcacaata ggcacccctg cgcttctagc aaagcgccta gcctctgctt
     3661 tatcacccat aagggatatg atttcaggag taggacctat aaacactatt ccagcctctt
     3721 tgcaggcacg agaaaactcg gggttttcag atagaaaccc atagcctgga tgtattgcat
     3781 ccaccttctt atccagagcc aacttaataa tgccttcaat atctagatat gcttcaacag
     3841 gacccttacc ctttcctatg agataggctt catccgcttt atttcgatga agagaaaaag
     3901 catcctcctc agaatagata gcgattgtgg taattccgag ttctgtacac gcacgaaaga
     3961 cacggattgc aatctcccca cggtttgcaa ccaaaactct acgaaagggc ttgacactaa
     4021 tccttttttt ataagtacgt cggttcaatt tacccccttt ttctacttat tacctatgaa
     4081 gagtttaata gaccgatata tttttctaga tataacatta ttaacttcaa ttgaattcat
     4141 actataaacc tgcttgatta tatttttttc taggaaacgg aatgtcatag atgatcaatt
     4201 taattataca atttttaatt actgttttga agtttctcat caaaagttta aaagaagtgg
     4261 gacgctctta tacatattaa atgggttaat gatgattcat acatctgata tcttcaaaac
     4321 cgattgttta taaaggtata atatagttaa agccaattta taaatataag atatctaaat
     4381 tcaactaatt tataaaataa aaggatagtt gaaaaaatga tagaaaaaag attttccaaa
     4441 ggggaagcca taaaatttgg gtggagcatg gtaaaaagca atctcatatt ttttgtgggc
     4501 ctattcattg cggatggact aatttatcaa gctcttgaga tcattagtaa gtcaaccaaa
     4561 gaaaatattc ctgtcctttc gtttgctttc gatattgttt atctagttat acaaataata
     4621 gttcagatgg gattaataaa aatctcctta agaatctgtg ataatatgag gtgcgagttg
     4681 gctgattttt tttctgattt tctatcctgt ttgccgcttt tctttaaata tttatccggc
     4741 atgattttat acctcttgat cgttcttatt ggcataatcc tcctcattat cccaggcgtt
     4801 atatggagcg ttaaatatag ttttgtgggc tattttattg tagacaaggg ccttggccca
     4861 atcgaggctt tgaaagaaag ctcattaatt acaaatggtg ccaagcaaga tttggttctc
     4921 ttttggttac taatttcagt cgttaatctt cttggagtac tagctttctt tataggtctc
     4981 tttgttacaa ttcctataac ggtagttgct ataggttttg tctacagaaa actactaaat
     5041 aaaaccgaag ctccgcatat aacagcttta ttaattagtc cttgattttt gtttaggtat
     5101 gtggcttttt gttgtttggg tggtctaaat tttacaatag cggaaataaa tatagagtta
     5161 attttcctaa cttaattaaa ataatatcaa gatagacatg ctaggattaa taaaaagcgt
     5221 aaattcaata ttgactgtga catactataa caattaaact ttagtttaaa tgctttttcc
     5281 atcttaccgt cccagaagac taagaaaaac caagaatatt cgtgagatta ttcaagaaac
     5341 caatctctct gtaagcgatt ttctatatcc aatgtttatt atccccggta atggtgttaa
     5401 agaagaaata acgtctatgc ctggaatttt tagatattcg attgacaatg caattaaaga
     5461 gataaaagaa ttaaaagagc ttggaatcca agctgtacta ctttttggtg ttcctgagag
     5521 aaaagatgag gttggatctg agggttacag tgaaagcgga attatacaaa aagctctaag
     5581 aaggattaaa gaagagataa atgacattct tctaattaca gatgtctgca tgtgtgaata
     5641 tacaagccac ggtcactgtg gaattgtaaa agatggtgag gtggaaaatg atgagacact
     5701 tcagtatcta gggaggatag cactctccca tgcaaaagca ggggcagaca tagtagctcc
     5761 atctgatatg atggatgggc gggtaggtgt aataagagag gtactcgatg aaaacgggtt
     5821 tcaaaatgta gctataatgt cttactccgc aaaatacgcc tctgcttttt atggaccgtt
     5881 tcgggaagcc gctcattcga caccacaatt tggagatagg catggatacc agatggaccc
     5941 acccaatata agggaggcat tacgagagat ctccctcgat attcaggagg gtgcagatat
     6001 tgtaatgata aaacctgccc tttcttatct cgatgtgata aaagcggcaa aggaggaatt
     6061 tgattaccct attgcagcat atagcgttag tggtgagtat tcaatggtaa aagctgctgg
     6121 aatgcttgga tggattgatg aaactaagct catcatggag attcttataa gcataaaaag
     6181 agctggtgca gatattataa ttacatattt tgctaaagaa gctgcaagga ttctgaactc
     6241 atcaaaaaga taacgtcaaa tttagttatg aagatatatg attataatgg tcaggtggaa
     6301 gacttctgat ctgccattag caatattata attttaattt gttaagcgaa tgagcataat
     6361 accaattctt ccccatggaa acctaaaggt ttccgctaca aattgtagag gaaggcttta
     6421 gccttctatt tcatttatca aagataggtc actagtattg tttccgaatt tatattcgat
     6481 ttctataagc ctagtattac aataagaaaa cacatgaaaa ataccctcta taaagtcgtt
     6541 gaagtcacaa ctgtcacaga cgaagagtta gaaagtgtaa taaatgaatg ggttcaaaag
     6601 ggctggtctt tagacacaat tcaatttgca atgagggaag cctctaaaag acctgctatg
     6661 gcatttgtaa tttttacgaa aagcaatgat agtgaggaag gttgaacctc aaatttaagc
     6721 accggctctt atacgttact caactggacc gctaactgta tcccaattaa caagaattag
     6781 tccaagcact atgagcacaa tacccgtaat ccgctgaata gatatggatt catgaagaaa
     6841 aataaaggca taaagtgcag tgaaaagcgg gaatgtagcg gcaataggag ctatccttga
     6901 agtaaggtct tgtttaagcg caacaaaata aataaataat ccaactatgc cacccaatag
     6961 ccccgctagg catgtccata aaatcgtttt tccatctagg ttacctatat ctttatactt
     7021 gccacttact aaagatataa taacaacaaa tgtagctgta aaaatgaacc gcactgtcat
     7081 aacagtaaaa ggagaccctt ttactaaact tagtttttca agtataggtg taattcccca
     7141 aattgatgcc acaaataagg ctaatgctgt tgctttcatg atgtacctcc attaaacatg
     7201 tactttaatg attattttga ggagcagaaa gataaagaga tcttatttta aagcttttca
     7261 ttaattttaa agtataagac tacttctgaa tgctaatgat taccattaag tgtctaatgt
     7321 aaccaatctc aatcattatc tataatgctg ataatctgat ttaagttttc taccctatct
     7381 acatcacctc tatctttaca tcttccgcct ttatcaatta acagcgctct aataccagct
     7441 gaccttgctc catagaaatc atagtctaag ctatcaccta tgtgaataca atgaaatgga
     7501 tgtacttcat ttctctcaag tgctattgaa aaaatctcag ggcttggttt tgcggatcct
     7561 acttcgcttg agataacaaa agaatcaaag tattcaataa tgccaagatc tatacaaacg
     7621 tcgtatattc ttgaatcgaa attagatatt atccctagta taagcccttt ccctttgagt
     7681 acagttaata catctttagt ttctggaaac aattcccatg ctttacttct aaaggtttca
     7741 aatagttcgt caaaataatc gtcgaatttc acaaacatgc caacttctga aaatactttc
     7801 cttacaacct cataccacca tttcttctca tagacttttc tgtcatcggt agaaacatcg
     7861 gcaaaagcta gaggaggagc agacttaaaa gctttaataa aggccttctc gatttgttca
     7921 ggtgtagaat tagcaccgta ttttcttgcc acactccaat acatattccc taccccgccc
     7981 tttatgcgga aaagcgtatc agcagcatcg aaaaaaacag ctttaattcg gttatttccc
     8041 ttcatgatca tatctacatt aataaatatt tgctgtttag gttgcaattc taatttatga
     8101 ctgaccacca gcaagactaa tggcctaccc actattttta taaccgacca aatcttatgg
     8161 ttaggtcacg agtcttgctc gtgaccgttt atactgaata tgctttattt cagaatggta
     8221 taatatacac ttgaaataaa gagtaaagca aaatgggtgg aacaaaccag agaacaaaaa
     8281 taattgctgt attagatgac ctatttttct cttctaaaat aaaagaagct acaagcaacc
     8341 tagaattaga cttaaaattt ataaaaaaca ctaatggatt aatccagaaa ctaaaattcg
     8401 agaaaccttc ccttattatc tttgatctta actcgaaagt atgcaatcca ctcgaaacca
     8461 taaaagagct taaatcgtca cccgatctaa aagacatccc tattctcggc tatttatccc
     8521 acgttcaaac cgacctacaa aacgaagccc ttaaagcagg atgtgaccat gtacttccaa
     8581 gatcaaagtt ttctaaagac ctgagggaaa ttttagcaaa gtattcaacc tcacactaaa
     8641 aactcaattt cttgatcaca agtatttttt acacaaatct ttctaatgcg atagacagta
     8701 ttaaaatttt catttagatc gtataacata agctgggtcg acaataaata ggaacatgta
     8761 tggaggactt accaactgaa cttgtgttca aaaacgatcg tccctttata aaatgctatc
     8821 gtgtcttatg gtcgcagtgt ctggttctcc tgacgtgttt cgtcctgata atcctatgaa
     8881 ctttcaacca aatgtgacaa tagagcttac gggtgctgtt tatccaagta tgagtattaa
     8941 accacatcaa attcatagaa atacagtaga gggtttttct caacggatag ttttaaatat
     9001 tctttatgta tgatgttcgc taatcatgtc tacgaagctg taaaggacga aaatgtctca
     9061 tcaccagagt tcgagttatt ccggcatatt cgtaatgcga gctcacacaa taaccgattt
     9121 aacttcatag atagcgaacc agcatgtcct gccagctggc gaggattgaa aatcgacaat
     9181 accaacaaag gcaaaaaaca atctcctata tgggtgggct tgttttggtg actttatggg
     9241 cacttcggac ttgcttttac tgttgtggga aattgagcaa atcataaccc gatctagctg
     9301 ttagccttgg taatcgccta caacgattgc ataacttcaa gccttagata ttttttgttg
     9361 ttcttagaga cctgcactat ctagagggat tttatttagg tattgttgtt tttttatcgg
     9421 atttaacaaa atcacctctt tcatacccca aattcggctg aagccatttc tcaacgacct
     9481 caacaagcat attcttgcgt ctggcgtagt cttcaacctg gtcacggtct atttggccaa
     9541 catggaagta ctttgcttct ggattggcga agtacaaacc tgaaacagag cttggaggat
     9601 acatagcgaa attttctgtg agggttattc cggtgttctt ctcagcatcg agcaccctcc
     9661 agagaatgct cttctctgtg tgatcaggac aggctggata accaggtgct gggcggattc
     9721 ctctatattt ctcattgatc aagtcttcga aggagagatt ttcatcttta ccaaatcccc
     9781 aaattattcg tgcttcctta tgcatcattt ctgcaagagc ttcggcaaat cgatcaccca
     9841 gtgccttaac cattatggca gaatagtcat cgttcttagc ttttaaggtt ttggcaaagt
     9901 cctccacctc ttctccagca gtcactgcga atccaccgat gtaatcctta tgaccagact
     9961 ccttaggtgc aacaaaatct gccagacagt agtaaatacc attctcatct tttacctttt
    10021 gctgacggat gaagtggaat gttcccaaaa cttctttgcg tgtttcgtcc gtataaacct
    10081 caatatcatc gccaacactg ttggcaggcc aaagagctaa aacagcgcgg gctctgaagc
    10141 gtttctttgt aataatttcc ttaagaagtt tctgggcatc ttgataaagc tcgcgtgctt
    10201 ggactccata atccttatgt tcaaaaatct ttggataaac tcctttaagt tcccacgccc
    10261 agaatagagg cgaccagtct atataaggga caatctcttc aagagggatg tcttgaaaaa
    10321 cctgcaagcc aaatttgtcg ggtgaatcaa tctgtatatt cttccaatct gtcttaaatt
    10381 tatttgcacg agcttctttt ataggaacaa agtttctttt tttcttacct gcggcgtatt
    10441 gttgtctttg cttttcctgc tcaactcgga gctcagcgac atacctatct atcttttctg
    10501 ggttcagtag attgttacag acaccgacaa tcagggaggc atcctgaaca tgttcgacaa
    10561 ctccgccata gtgaagagca atcttaatcg ctgtatgggt tctgctagtc gtagcccccc
    10621 caataagaag gggtatctta aaaccctgtc gctccatttc agtagcatta tggatcattt
    10681 catcaagaga tggcgtgata agcccactta aaccgataat atcagcgttt atttctctag
    10741 ctgtattcaa gattttttca cagggaacca tcacaccaag gtcaataact tcatagttat
    10801 tacatgcaag aacaaccgca acaatatttt ttccaatatc gtgcacatca cctctcaccg
    10861 tagcaataac aaatttcccc tgatttcgtg cgttttggat cttgcctttt tcagtctcca
    10921 taaatggttc gagatgcgca acggcttttt tcataacgcg cgcactttta acaacctgag
    10981 gaagaaacat cttgcctgct ccaaaaaggt ctccaactac cttcatgcca tccataagtg
    11041 gcccttcgat aacatcaaga ggcgttgcgt attgcagacg ggcttcttcg gtgtcctctt
    11101 caatataatc cataattccg ttaatgagag catgtatgag tcgctcgttt acggtgcttc
    11161 ttcgccagag gctttcatcc tttactttag ctttgcccat gcctttgacc ctctccgcat
    11221 attcgataag gcgctcggtt gcatctggtc ctcggttgag aagaacatcc tcaactaatt
    11281 caagtagttc tttatcaatt tcctcatata cattaagcat gccggcatta acaatagcca
    11341 tatcaagtcc tgaattgatg gcgtggtata gaaaagcgct atgcattgct tctcgtactg
    11401 tattgttgcc gcgaaaagag aaggatatgt tgcttactcc tccgctagta cgtgctccac
    11461 gacatgtggc tttgatttca cgtactccct caataaaatc caccgcataa ttgtcatgtt
    11521 cttcaattcc tgtggctacc gtgaggatgt tggggtcaaa gatgatatcc gtcggatcca
    11581 tgccaacctt ttcggttaag agtttatatg cacgattgca gattcggacc ttatctgctt
    11641 tgttggcagc ctgtccatgt tcatcaaaag ccattactat gacggcagca ccataacgca
    11701 ttattagtct tgcctgttca agaaactttt cttcgccctc cttcaaactt atagaattca
    11761 ctatggattt gccttgaaca gagcgaagac cagcctcgat tactgaccac ttggagctgt
    11821 caatcattat tggaacacgg gcaatgtcag gttcggaacc aatcaagttt aaaaagcgtg
    11881 ccatgcatgc tttgctatcg agcagagcct cgtcgaaatt gacgtctata atgttggcgc
    11941 cgttttctac ctgctgtctg gctatagtga gggcgccatc gaagtcatcc gctttaataa
    12001 gtttagcaaa ttgaggtgat ccagttacat tggttcgttc tccaatcatg ataaatggag
    12061 atttgtcgcc ttctattttc aaaggttcaa gaccgctgag acgagtcgca gaagtaactt
    12121 ttgggatttt acgcggcgat attactctca atctgttagc aatagcattt atatgctcag
    12181 gcgtcgttcc gcaacaaccg ccaacaatgt tcaaaaatcc actacgtgca aaatcttcca
    12241 ataaacttga ggtaatatca ggggtttcgt catacccagt ttcactcaaa gggctaggaa
    12301 gaccagcatt agggtaacaa ctaatatagc attctgcgat tctagaaagt tcttctatat
    12361 gtggacgcat ttccttggca cctagagcac agttaatgcc gacactcaag ggtttagcat
    12421 gcataacaga gttccagaag gcctctatag tctgtccaga gagagtgcgg ccagaggcgt
    12481 cagttaccgt tacagagagc ataattggta gacgaaaggg gtactcatca aaaaactttt
    12541 caatagcaaa aatggcggcc tttaggttaa gggtatcaaa aacggtctct ggcaataata
    12601 tatccacccc accatcaaca agtcctttca cctgctcata gtaagcatca accagctcct
    12661 ggaacgtaat agaacgaaat gaaggacggt taatatccgg agacattgag gctgttctat
    12721 tagttggccc aattgcacca gcaacaaagc atttccgttc tgggttcaaa gccatgaacc
    12781 catcagccac ctccctagct atccgagcac catctctatt cagttcataa actatatcct
    12841 caagcccata atcagcctgg gaaactcgcg tactattgaa tgtattggtt tcaataatat
    12901 ctgcacctgc ttctaagtat tgacggtgta tcacagcaat tatctgaggg caagttagat
    12961 ttaagagatc gttgtcccct ttaagctcct tccaatggtt cttaaatcgt actcccctaa
    13021 attcggcttc ccctaattta tggccctgaa tcatagtacc catagcaccg tccaaaaaaa
    13081 gtatgcgctc tgcaagtaat tctctgagga gttgaaatcg ttcgctttca gttcttctta
    13141 acatattagt aaagtatatc taaaggactc ttaatcctcc tccgactcca gaatctctag
    13201 tgctagtttt acattatttg ttatcataac gatagctttt ccggcttcct caggcaaacc
    13261 gtctatatca acctctgtag ttagatcaag catcaacgca tctatcttac gtaaaaacct
    13321 ctttgctgta tcaattttag tcatgtttaa gcctccttcg aatttcaaga ttttatcgat
    13381 tttattcctg aacaagaaat atgagtggta aaattatgca cttctaataa gccttctaca
    13441 ttcgtcaaat agactacacg ggaactgtaa attctttaac aaagcttcta aatctataaa
    13501 gcttcctgaa agcataagct ttatactcaa tcttcttcca tgaacaagaa caaataggtc
    13561 ttctatctca gagaatttag caaattcatt gtttaccgct tcaattattc ctttgagaac
    13621 tatacgtgaa tccataatcg ccacttctat aggaactcca ataaacagca ttaagtcatc
    13681 ttgtttcata tcacccctcc ctgttcgcaa atcgtggctt gttaaccgtg aatcgtatat
    13741 cctaaaccct gtatcgaatc acaatccacg aatcacgatc aacatattag tatgccataa
    13801 attccatttg actcttattg tagaaaggat ttaatatata tctcaaaaat ttggggttta
    13861 aatatgatta aaaaagtaat aaagaggcat gcaaaagtaa aagaacgtca gaacctatct
    13921 ttattcacat tagttttagt ctttttagta attttgatta ctgattgtaa taaaaaggaa
    13981 aatgtaattc cagcagcgca tatccctgga ataagggcgt ctattacgaa aatactcatc
    14041 tctcccctag ccttcgatgg cgcaatagtg gctgtcgaag gaatagccca cgatgttaag
    14101 caagagaaat ctgatggaaa agataagaca tcaactatat ttaagttatc tgacctccga
    14161 ggaaattata taaatgtatc aacgccagga aaaagggaga ttttcgaata cgattttctc
    14221 gttgttggag gaatatatcg acgagctgaa aacttaatag aagcacaaca aatagagata
    14281 gtattattag aagaaaaata gcatcactta ctatgttaca ttcaaatctc gatttgtatt
    14341 tactaagtaa aaccttgctt acaacttgtt gttttccctt aaataaagat cttagaaagg
    14401 tactcttgaa ggtattcagt attaattagc tgactcttaa agccaacgta cccgtcagga
    14461 cgaactaaat aaaggcaatt tgacctagct ttatatttat gatggagcga tttcttgtag
    14521 tcgagcaaag ttgagcactc aaaactaact tgcggagtct caaatgctag aaccaagtat
    14581 acagtgatat actgaccgta tttgctttgt attatcttac caatccttgc gagattctga
    14641 ttacactctg aactaatatt gctcccacta aaaactagca acgtaaactt tgtaccacga
    14701 aaaagttcaa aaagccttgt atatctccca ccaggataca ttgtaaccat accatcaggg
    14761 gcacggtcac ccgcagctaa tttagttttg aacttaaaaa acgctttcga tactcatcag
    14821 caattggact acgtctgtag ttaatatcta gctcagaaac gatattcctc gcccgacgtt
    14881 gcacccattc acggtgtagc aataaaggtg caattattcg aacagacttt attacaattg
    14941 gactatttcc caaaaaggta cgaaatagca aatccgatcc cctaagaact gctttagcaa
    15001 caggatgtcg ctcctggtga tagctatcca gtaaaatcga acgtcctaca cccctcgtga
    15061 cgagcgccag tttccaagca aggttaaagg cgtcctgaat gcctgtattc atcccttgcc
    15121 cgcctgcagg actatgaatg tgcgccgcat ccccagctag aaagacgcgt ccaactcgga
    15181 gctttggggc aatccggcga tgcaaccgaa atccagacaa ccagaccgga tcacttaact
    15241 cagcatgcat atgcactctt gccttcatca tgttttgcat ctcttcaaga gtaggatctt
    15301 ggtaatcctt tgcgctagat ttatcccgag acgctattaa ccggtagcgc ttatctccgt
    15361 gcatgggtat cacagccatg attccgcggc ttgtaaaaaa gaaatatcct tgggccggag
    15421 gcatggtcca ttgcacttgc acatccgcaa gcagaaactc ctcttcgtat ggagcacctt
    15481 caaatggaag gtcaagtatt tggcgtacaa cgctatgcgc tccatcacaa cccacaagcc
    15541 ggctagcccg gagtacttct ttgtatccac cagaatgcct tattattgct gtaacacagt
    15601 cctcgtcttg tttaaatcta taagttcaac ctgacgctca actttcaagc ctaacccttc
    15661 aacatggttg gctaaaatgc gctcagtttg actttgctct aaaatcagag gaaacggata
    15721 tggactgtca agaccatcaa attgcatttg ggcaattaga cagctatcgg cgtaagcatt
    15781 tacgctacga gagtgatcaa atcatgatga agaatatgtt caatttcctt aacatattca
    15841 tctttgaaac tttttaaaca caccgctaca aatggaacaa gattccatag aatcttaaat
    15901 atcgcataca tcctgttttc aataccactt tggcttagaa tgatgatcaa tcattcaaaa
    15961 taatactaat aattcaaatt aagaatattg aatatgcgat tattaaccga taaccttaag
    16021 tacttggtat ggctgaccaa aaaactacac ctccgaataa taacttttct gaacaacatt
    16081 caaaaacgga atctattcaa ccacaaaatg acactcatat tccaccgcca gcagtattgc
    16141 acccacgtct tgtgcttgat acaagccttt ttgttaatcc tgatgcccaa cgtcaatttg
    16201 gtgaaaatgt gaatcaagca gtcaatagat ttctaaaaat tacacgagag aataacatcg
    16261 agatctatat gcctctctct gtctttcgcg aactctcaaa cttcacatca ccagaagcat
    16321 taataacttt tcgccgagat gcaattgtga gagcgcctga cctttataac ttgcaggtac
    16381 cggctgctat ttttcacatc ttcataggtg aactacgtaa ccgcataaac aaagggctaa
    16441 gaatcacaga agaagcaatt cacaaggaac ctaccgctga aaacatacgt agactaagac
    16501 aagaataccg tgaggcttta cgcagtggca tggtagatag cgtggaggac ctagacgtag
    16561 tcttacttgc aaaagaaatt gggggtgcag ttctatcagc tgatgagggc atcgccaata
    16621 tggcggaagc actcggtata gaggttttat cagctaccta ttttatccat agatatggag
    16681 cagaataaaa ccaagagtga aggaacatgc ctgatgtaga gcgcggggtt gaggagagag
    16741 aatataacgg gcttaaatac ttccggtatg cggatgatat tggcactact ctgagaggaa
    16801 cagtcgtctt attcgacact agtggacaaa gaccaatccg tactatacca ggatttcctc
    16861 atataaagcg agtttatcgc ttgcgaccag gtgtgcagcg ttttttccat acaaacccat
    16921 tttttgttga agaaaaactc gatggttata acgtccgact cctactccat cagggtcaac
    16981 ttttagctct gactcgtggt ggttttgtat gtccatttac ctctgagtgg gcagaaatct
    17041 ggtggcacag atacaatctt gaacgctttt ttacaaccta tccagactat gttttatttg
    17101 gtgaggtact cggagataac ccttataatt cgcaaaggga cccctctatt ccacctggat
    17161 tatatttctt cacatttgat atcatgagat cagatgggga atttttacgc gttcatgatc
    17221 gctatgagct tgttgagggc tatgaattgc ctacagtacc acttttagga caatttactt
    17281 ccgatcgtat tgatgagcta tttgggattt tgcgtgatct caacgaaaaa gaacgagaag
    17341 gtgtagtact taaaactaac acaagcaaaa aatctctgaa atttgtaact ccactcacgg
    17401 acttacgaga tattagagat aacctaatta tagaatttga tctagaacct ggttttatta
    17461 ctaatcggct tttgagaatc tccctttttg taagagagtt tggtttaaat gaggaggagt
    17521 acgcatctca agtcggtaaa gcctttctcg atggttattc taccctagag acttttgaat
    17581 cctcaaacga aacatataac atttatgtca agcttcctaa aacttggaac tcacttcgta
    17641 ccctgcttag cggttatgtc cctattaaaa ccgaccgcgt cgaaccagtt atactagatg
    17701 gactagagat gctgcgtgta gaattccgtc gagtttttcg taaaagtacc cgccgatacc
    17761 gtgaaatcct ccgaggttat ggtcatattg attaaataaa tgaattgtat gaaattgaag
    17821 aaatttataa tcctgcttct agaaccacca ttctatagga tttttttttt aacgtttaag
    17881 ttagctatta caaaagatct tgttaagcat ttcaattact gttcccaatt agttagataa
    17941 aataataaaa actaattcta ataaattaag ggaggttgaa atgatactta aagataaagt
    18001 tgctattatt acaggcggta caggggcgtt agggagagca gtcgtatcag catttttgaa
    18061 agaaggtgca aaggttgcct gtacatatat tgtggatgaa gaactgaaag gggtttccca
    18121 ttttattgag agttataaat cgcaaattaa gtttatcaaa gcaaatgtta caaaagaaga
    18181 ggaggttaaa aagactattc aagaaacatt agaagaattc ggaaagatag atattttagt
    18241 aaatatagtt ggaggatttg cctatgcaaa aatcttagat acagatgaaa aaacctggga
    18301 ttcaatgatg aatatgaatt taaaatcagc ttacctgtgc tcaaaagccg tattgcccca
    18361 catgattaag caaaattatg gaaaaattgt taacatatca tcccgtccag cattaaaggg
    18421 ttcggcaggg gttggagctt actccgcatc aaaggcaggt gtattaaatc ttactgagac
    18481 aatcgccgat gaagtaaaag actatgaaat taatgtgaat acaatcctac ctagcacgat
    18541 tgatacacct gcaaacagaa gagatatgcc taatgcagat ttctcaaaat gggtaaaacc
    18601 agaggaaatc gcaagggtga ttgtttttct tgcatctgat gattcaaaac caataagtgg
    18661 tgcagcaatt ccggtgtacg gaaaggctta aattctcacc ctctactcaa gctgtgacat
    18721 cagatttatc ttgaagaata aaatccagaa gtgttctacc gaagtctgag gcaacggcga
    18781 tgtcctcggg agtaattact acgttattta agtactggag cctgagaatg tctacatccc
    18841 ttagttctgg aataacgccg ccataaaaga aaccaatctt ccgtagttgt gttccaagca
    18901 ctccggttag atcatttgag agcggaaggt ctacgtatat acattcgaaa cgatttaggc
    18961 aaagttgttt aagatggaac ctgatttcat caaatgtttt ttggccaatc gcttccacac
    19021 taagtagagc ctggttgtgg tccggtcgca ttgatacttt gatacgtcca gtttgtcgag
    19081 ggcgttcatc agttcgatcc gcttttacat caacgatctc tctctcaatc cctaaaatgt
    19141 cgtatatttg cttaagaatc gtacgatagg cacctggtgg aaagataggg gctgttgcac
    19201 tcttgagaat cgaggtgtac atgagagcaa ttgactgacg cctaggtctt ttttgatctg
    19261 taatggttct aaacgaaact gtaccaggac tatagccgag taggaaacct acctcgtgag
    19321 ctcctaatgc caaatttcct ttctgactat aaggatgaac agtaactgcc tcaccatata
    19381 tgccgatgag ctgatttgcc acagcatagt caagaagaaa gtgcttaatc ctttcaaaga
    19441 ttttgtgtcc gcggtagcga ggatcaacta ctgcttgacc cgactctgca accttagccc
    19501 cagggtgatc aaaggtaagt gcgatgtgac ctaccatttc accccgactg ttataaacgg
    19561 caaaggattt catcagtcca gatcgctgtt tagcttcgac ctgctcagga tagtaaacaa
    19621 attccgatgg gtatgtatag ccgtagcatc gataaacgag gcgagccaac tctacagcct
    19681 catctggctg cattatacgc atgataagag cctcctcagg atgcgcttct accgcatcta
    19741 gggcttccct gtgctctgga ggatctgact gataacgaat gtcggttgaa ggaagatctt
    19801 taaaaagctc aacgtggtta ccggcgggac ctagatttat gaaatgaacc tggtcagcat
    19861 aaccctttga gaggaaagag gaaaaccgcg ggtcttcgcc ttgctctatt ctcaagtagt
    19921 caaagggaag tcctttatct ttgattgtaa cgacaagtga atgagtccgt ctggatacaa
    19981 tgacgttaac tgattgtgta gtatctcctt catatccata ttcaaaaatt ttctgaagga
    20041 cctcatttat tatggcatca agttttctgg cgctttcttc atctaaacca gtgactttag
    20101 caatatcggc gatcaagtcc gcaactgcat caaggtagcg gtcatctgct aagaccgtaa
    20161 gccgggcgat ttgaatctct gatgcgtccg tctgactgta aatcgaattc tcagtcataa
    20221 gtatttacac gtgaatattt agattttaac ttgagaattc cctgtagctt gctacagggt
    20281 ttccacaata gtgtattgtc attgcgaaca cgttcatttc gttcagtgta aactccgtga
    20341 agcaatctct cattttagat tgcttcgtcg ctttgctcct cgcaatgaca gctataacgg
    20401 tatattttaa gaccataccc tgcagcttgc tgcagggtta ttgattgatt gcataaaaag
    20461 attatataaa aagatatttc agttcaataa aaattgttgt agtttatttg gtctaaatct
    20521 tcatctcaaa tttatatctt ttaactcact tggaaagaat aatcaaggag ataacaaaat
    20581 gattcggcca tcgcatagat tatctatcct tttgttcgtt actttactgt tatcatccac
    20641 cgttacactt actaaggaac ctgagaacct tcatttagca aaacaagccg caattttcta
    20701 tcatgactca ggtgagtatg attacgatct tggtgtagtt gttcaagaag ctcagactta
    20761 tcttgaagaa aggattcccc aaaaaagaag ttaatggata aaagaattct tctagactga
    20821 gcgttccagt tgaatatgaa aactgaataa taacctacca ggaatatcgt gtgatgtagc
    20881 tctttgggag ttcccaaaac tttacacgcg gggaaaacca catgctatca tggataaaac
    20941 aaacatgtgt acagaaacat ttatctttgg caatagattt tacttcttca tctctaacct
    21001 tagaattcca taatgcttgg aagttacacc catgatctct cagactgcct attgaacccc
    21061 gtagttcaca ggctctgaca gttccatcat agtcgataac taaagacgtt tcaccagcag
    21121 tacaaggcat gggccaaggg gtattctcgg tatagttatt aaactgagtt cggttatgga
    21181 aattaaaaac agaagcgtaa attgattttc taaaccagcc gtttaccgca ccaccgttat
    21241 ctccctgtct tgaagcatat accttctgaa tctttgttat ttctttataa agactcctta
    21301 attcctctgt aggaaccttt tttaaacttt tatcaagtgg gtcgccgcga attatttgaa
    21361 agtaatgata atcaagccgc tcattatcta gaaaaaacct accaagttct acaagctcat
    21421 tataattttc ggcacagatc accgaattaa catttatttg aagtctagga ccgaatctcg
    21481 gctttaaagt ctgagtaaac cttatggtct caagagactt attaaaattt cctgggacac
    21541 tacgaatcct atcatttgtc tccggtaacc catcaatcga attatttaaa tagacctcaa
    21601 gctcgttgtt ttcttgtaaa attctcccga ctagctctcg tatacgctgg ggcttaagac
    21661 catttgtagg gaaatagatt cttctaatcc cattatttct gtaaaaaaga tttactatct
    21721 ctggaagctc atttcgaaga gacggttcac caccactaag aagaagatca tgaaaatgag
    21781 acattgtctc agatagtatc ttaatttcat caaatgataa atcacctttc tgattaagct
    21841 cttgccagta gaaacaggtc ctgcatagtg cgttgcatcg agaagtgaca aagaatatta
    21901 cagtatggag cttttgttgc ggtcttatga attcaatgag ttttttcatg gcgataaaac
    21961 ctaactccat tatacttttt gtaatagata attacaattt atatataatt gttttttgct
    22021 agttgtgtgt taattcaagt gcaatagcgg taatagcagt ttgttaccca atttaaagtg
    22081 gaaaaaattt tgactcatta tacatttcct acttagctta tattttgata ttttttagat
    22141 tatctttaat gtagactaat atcaccatga aataaataat ggaagtatga taacaatgga
    22201 acagatgttt ttagcaattt ttatagacct caataatatt ccaagcaaaa taaaactgga
    22261 acgtcttatg aatgcattaa tactaagtga acaatcaaat tacaatttta ttttcgcagt
    22321 taaatctgca tatggtgtac tacactctgt ttctcaagag ttcaggacag aacttagaga
    22381 ctataatttt tctatcctgg aaacgccgcg cataggatta aaaaagaata gagctgacct
    22441 atttatcagc attgatgcat ttgaaacatt atacgtcaac aatcccggaa tagatcgtta
    22501 tatatttgtt acaagtgact cagattttac tgttataatg gataaactca gaaaatacgg
    22561 caaggaggtt tggctcgtat gtcgtaaagc agatgaaaca agacaaatac tagcaaaatc
    22621 atgtgacaag atgctattca tcgaggattt ttatcaattt gaagatgaaa aatcacacaa
    22681 agaatatata gaagccaatg ataacaaagc gaaggaactg ttcaaggagg ttttgagaaa
    22741 tatagactct gaaagacttc catttaatat ttctgttatt aacgatagaa tgaaacaact
    22801 ggatagaagc tttgacgtaa gacatacatc atataaaaag tttattgaat taatcagagc
    22861 atttcacaca ctagggatta taactatcga ggagagcgaa tcaggtgtgg taagaattac
    22921 ggatatagac ttatcaaaga tagaagtagg aaattcaggt tctaatctta ctaatgattt
    22981 atcataaccg ggctattaca ttgtaaaata caaagcgaat ttacttctac tatgagcgga
    23041 gagatacact gagatagtgt taactaaatt aaaaaataat catacactag gaataaatgt
    23101 actgactttt atcctatcag ccatccccat aatcaactat tagagatacg cttacaaccc
    23161 aaaaaacgtc ttcatacttt gcaagaatcg ttcagtataa ttaatgcgca taaccgcaac
    23221 ctcttcggct ttttttcctt ctttgaaaaa gccctcattt atgagctgcc tttccgccaa
    23281 acgcttgctt aagtcctcca taataccagg attattttct aggataggta gaatattcgc
    23341 cttttcaact tcaaccactt ccaggtcggt ctccgctaca acacttgcac tccttaattc
    23401 acctgtaaga agagattttt ccccaaaaaa atcccctgta tttagatgtg ccacagtaac
    23461 tatccttcca tcatcaggta tctttatatc aacagccgca ctccccccca gaacaagaaa
    23521 gagtgaacta ccagcctcgc cctgtttaac aatatgctca cccctaccaa agtgccagag
    23581 ccgaccagtt gatgctatgg tttcaatctc tgtatccttt aaaggttcaa aaatgcttac
    23641 tctgcggaga agctcttcta ttcttaccct cctacgattc atttcttcaa ttgcttgttc
    23701 ttttcctact tcctcatgaa taaacacatc gcgaatcggg aaaggaatcc tgatattatt
    23761 tctccttaag gtataccaga tgcggatcat cacatcactt tgaatatctt ctgatatggg
    23821 tctatcctca atgaaatact ttagttgata agttatagcg aaatctccgt agttaaccaa
    23881 tcgaacgagc ggtcttggtg tagatactac acctctggtc tcaagagcac agttaattaa
    23941 tatctccttt actagattag gaagcgcagc atattctaca cccacataca aaagtagggc
    24001 atgaagtctt gtaggtctat agaaattcgt tatttcttcc ttagaaatta tgctgtttgg
    24061 aataacacga tagttaaagt cccgtccccg aattgtagtt gcgcgccaat ttatatctac
    24121 tacttcccca tctttatctc caacctgtac ccagtctcct acctgaaaag gtggctctat
    24181 attgagtgca attccagcaa tgataccacc tagaaagtcc tgaagtgcaa atgcgagtaa
    24241 cccagccgta acacttgaag caacaagaag ccctgaaagt tgtttatcaa aaacaaatcg
    24301 aacaataact aatatagcaa tgattaagat tagaacggta actacactcc tgaggagtcc
    24361 cgggacatgg gttttgcggt attgaaaaag ataatagtcc cagaagaaaa cagagaatat
    24421 ctttattaga aaccatactg caaagaaaac aactagagct gtataccact taatgaatgt
    24481 atgaagcttt acctctttga gtaagccgta tttgtggttt aaagtaagcc aaagcccaat
    24541 tgcaacaaaa atggaaaaga ggacatagga taatcctagt cttatcttta aacgggttct
    24601 aactatccag ccaaatccta aaagaacaac taatataaaa aaactgataa gggatgtaaa
    24661 aagtattgga gaattctcaa gccacgtaat catgggatta gttgttagtt attttatagc
    24721 cttttcgaat ctcttacttc ttaactcaac aaaagatata tctacattca ctgaaggcat
    24781 t
//
LOCUS       DATDRL010000284         3868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_110015, whole genome shotgun sequence.
ACCESSION   DATDRL010000284 DATDRL010000000
VERSION     DATDRL010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110015"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VI935_09570"
     CDS             <1..336
                     /locus_tag="VI935_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HLE25881.1"
                     /translation="KALIYSGYNVIDKEVKVLPDKIKGNMDVEIAFRLLTEIDRYDEL
                     VFFGGDSDFVVVFNHLKNLGKKILCVGRPDMTSLEIRNVATAFHDLNALKGYLVMKER
                     RSGNGNRQG"
     gene            317..547
                     /locus_tag="VI935_09575"
     CDS             317..547
                     /locus_tag="VI935_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25882.1"
                     /translation="METDRDEQLKTRIDWLRRISTHTVVSMKVLKVVEDERQRIAELV
                     ASHREKDGAKVSMASSPFYGLTRQITSFSQAV"
     gene            858..2876
                     /locus_tag="VI935_09580"
     CDS             858..2876
                     /locus_tag="VI935_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLE25883.1"
                     /translation="MTIAKKYKIEVKRDHLSKIATGSPESALAEIVWNSLDADAIKVE
                     INFHEGALGVDEILISDNGNGIVYEEAENLFVSLGGSWKARQQKTGKGRFLHGKNGEG
                     RFKAFVLGHIVDWDVVYKKGNKYMQYTIEERADSLDEFTLGEEKPIETDQTGVQVRIS
                     ELSKKFHILTKEKAIEKLTPIFALYLSNYPQINLSIEGSRINPDEVIRHKTSHTLDSI
                     KVDGSVYPVELEIVEWNGISEHELWFCNENGFPLELYNRQIRGIGNFGYSGYLKSAYL
                     QYLHSENLLALGELNKPIEQICDQAIKQIKDYFLKRTLEGASAQLEKWKEEKVYPYAI
                     EPQTPVEAAERQVFDIIAINVNQNLPDFEQTEKKTKAFQLRMLRQAVEKSPEELQIIL
                     GEILQLPKSKREQLAELLKNTSLSGIISASRLVADRLKFLSGIEHMLFDDDIKKHFKE
                     RSQLHRILADNTWIFGHEYSLSVDDKSLTEVLRKHIARCEKNIIIDEPVRRIDGSVGI
                     VDLMLSRSIPRNHANELEHLVVELKAPSVPVGQKAIEQIKSYAFAVAKDERFRGLYAR
                     WHFWVISNDVDDYAEVELSQEKYEDGVIFKTTKDIDITIWVKTWSQLIRENKHKLEFV
                     REKLNYNIDREDALKYLRKTYAEYTQGLILDKEDEKITADYTEEAENA"
     gene            complement(3222..>3868)
                     /locus_tag="VI935_09585"
     CDS             complement(3222..>3868)
                     /locus_tag="VI935_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25884.1"
                     /translation="ADKPASARYALVDMILNADKMVSLLKRQVGFNLSFDIARNLLEN
                     LNDFIDHLWANVIKTSPHLYSFNKRNWRDLNDTIDIKLKLIEGRNFVNVIDPRSEEIG
                     QIAIAVKILENHILYVRTTGSFDKMEETINIYRNTIEEFDSFLISLAKTIKFDYRSSL
                     KKHEFGEDGQKTDRTAWPLLSEETTEATNSVEQAEKEEKADPKKRRKMGLPGTT"
BASE COUNT         1136 a          703 c          942 g         1087 t
ORIGIN      
        1 aaggcgctga tatacagcgg atataacgtc atcgacaagg aagtcaaggt gctgccggat
       61 aagataaagg gcaacatgga cgtggagata gcttttaggc ttctgaccga gatcgacagg
      121 tatgacgaac tggtgttctt cggcggggat tccgatttcg tggttgtgtt caaccatctc
      181 aagaacctgg ggaagaagat cctgtgtgtt ggacgccctg atatgacgtc tctggagata
      241 aggaatgtgg ccacggcctt tcacgatttg aacgccctga aaggatatct tgttatgaaa
      301 gagagacgca gcggcaatgg aaacagacag ggatgaacag cttaagacta ggattgactg
      361 gctgaggagg atttcgacgc acactgtggt atccatgaaa gttcttaagg tcgtggagga
      421 tgaaaggcag aggattgcag agctggtagc tagtcatcga gagaaggacg gggccaaggt
      481 ctccatggca agctcgccct tctacgggct tacacgccag attacgagct tttctcaggc
      541 ggtatagtga taggcatggg cctgagtttc gagactatca agagccgatc cgttacggtt
      601 agttaccgta ttcaggaacc tgactccgtt cgtcgctggc gttgatatat aagaaatctt
      661 tgtgagcacc aactcaagat cggccttttg ggatatactc ggctagccat cggtgcttga
      721 acccatccaa ctaaaagatg cagtgcgcta tagatttctg caattggctc ttgaagatta
      781 tgctgaaata ttgcaaacta caacttaaaa tcaacagcca gaatctattg caataatatt
      841 gtgaaggaag cccgtaaatg acaatagcaa aaaaatataa gattgaagtt aaaagagacc
      901 atctttcaaa aattgctaca ggcagtcctg aatccgcatt ggcggaaatt gtctggaatt
      961 cactagatgc ggatgctatc aaagttgaga taaattttca tgaaggagca ttgggtgtag
     1021 atgaaatact gattagcgac aatggtaacg gaatagtgta tgaggaggct gagaatttat
     1081 ttgtttccct cgggggctca tggaaagctc gccagcagaa aactggaaag ggtcgttttc
     1141 tacatggcaa gaatggagaa ggaagattta aggcgttcgt tttgggacac atagtagatt
     1201 gggatgtcgt ttataaaaag ggcaacaagt acatgcagta cactatcgag gagcgtgcag
     1261 attctctaga cgagtttacc ttaggtgaag agaagccaat tgagactgat caaactggcg
     1321 tccaggttag gatttcagag ttatctaaga aatttcatat attaaccaaa gaaaaagcta
     1381 tagaaaaatt gactcccatt tttgccttgt acctgagcaa ttatccgcaa ataaatctaa
     1441 gtatcgaggg ctcacggata aatccagatg aggttatacg acataagacc agtcatacgc
     1501 tggattctat taaagtggac ggttctgtgt atcccgtgga acttgagata gttgaatgga
     1561 atgggattag tgagcatgaa ctttggtttt gtaatgaaaa cggatttcca ttggagcttt
     1621 ataacaggca aattcgcggt atcgggaatt ttgggtatag cggttatcta aagtctgcat
     1681 atctacaata ccttcattct gaaaatttgc tggcactcgg agaattaaat aagcctatag
     1741 aacaaatttg tgatcaggcc attaagcaaa ttaaagacta ttttttaaaa agaacactcg
     1801 aaggtgccag cgctcagcta gaaaagtgga aggaggaaaa ggtctaccca tatgcaattg
     1861 agccacagac ccctgtcgag gcggcagaaa ggcaagtgtt cgatataatc gctattaacg
     1921 taaatcaaaa ccttcctgat tttgagcaga cagaaaagaa aacaaaagcg tttcagttgc
     1981 gaatgcttcg gcaagctgta gaaaaaagcc cagaggagct tcaaataata cttggagaaa
     2041 ttttacagct tccgaaaagc aaacgggagc aattagctga actactcaag aatacatcac
     2101 tatctgggat tataagcgca tctcgtcttg tcgctgaccg tcttaagttt ctatctggaa
     2161 ttgagcatat gctttttgat gacgacatca aaaagcattt taaagaaagg tcacaattac
     2221 atcgcatttt ggctgataac acttggatct ttggtcatga atattcttta tctgttgacg
     2281 ataagtcatt aacagaagtg cttcgcaagc atattgcgcg gtgtgaaaaa aatatcatta
     2341 ttgatgagcc ggtaaggcgg attgatggta gcgtgggtat tgttgatctt atgctgtctc
     2401 gttctatacc acgaaatcat gctaacgagt tagaacatct tgttgttgag ctcaaggcac
     2461 cgagtgttcc agttggacag aaggcgatag agcagattaa aagttatgcc ttcgctgtcg
     2521 caaaggacga gcggttcagg gggttatatg ccagatggca cttctgggtt atatcaaacg
     2581 acgttgatga ctacgcagag gtggagctga gtcaggaaaa atatgaggat ggtgttattt
     2641 ttaaaaccac taaagacatt gatataacta tttgggttaa gacttggtca cagctgattc
     2701 gagaaaacaa gcacaaatta gaatttgtta gagagaaact gaattataat attgaccgtg
     2761 aagacgctct caagtacttg agaaaaacat atgcggaata cactcagggc ctgattcttg
     2821 ataaggaaga cgaaaaaata acggcagatt acactgaaga agctgagaat gcttaataga
     2881 cgccgttaag actgtccgga tgaacttgtt tgttatatat gtttcattca gtaagtttta
     2941 tttttttact ttttaagtga aaaacctgtg tattaaataa gaagccactg atagcagtca
     3001 tcggtattta gcatgtttta gaagtatgag agttgcattt ttctggtcag aactggcgat
     3061 aggaattaat cggaataagg tggcatgttt caccgaaatc cgcagcagat attgaagcgt
     3121 tataggtgtt tacttgctta gggtgctgaa aaaatagaaa ccccgattac ctttttagcg
     3181 taatcggggt ttttttatat tcaaaggccg ccggttatat tctatgtcgt gcccggcaac
     3241 cccattttcc gtcttttctt tgggtcggcc ttttcctctt tttccgcttg ttccacggag
     3301 tttgttgctt ccgttgtttc ctcggagagg agaggccatg cggtcctgtc cgttttctgc
     3361 ccatcctctc cgaattcgtg ttttttgagg gaagacctgt aatcgaattt tatcgtcttc
     3421 gcgaggctga tcagaaatga gtcgaattcc tcaatagtgt tccgatagat gttgatggtc
     3481 tcttccatct tatcgaacga ccctgttgtc cggacgtaga gtatgtgatt ttccaggatt
     3541 tttacggcga tggcgatctg ccctatttcc tcgcttcgtg ggtcaataac attgacgaaa
     3601 ttcctgccct ctataagttt cagctttatg tctattgtgt cgttgagatc gcgccaattt
     3661 cttttgttga aggaatagag atggggcgag gttttgatga cattagccca gagatgatca
     3721 atgaagtcat tgaggttttc gaggagattc ctggctatat caaacgatag attgaagccg
     3781 acctgtcttt tgagtaaaga gaccatcttg tcggcgttta aaatcatatc aacgagtgcg
     3841 tacctcgccg atgccggttt atcagccg
//
LOCUS       DATDRL010000285         4640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3518306, whole genome shotgun sequence.
ACCESSION   DATDRL010000285 DATDRL010000000
VERSION     DATDRL010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3518306"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..1546
                     /locus_tag="VI935_09590"
     CDS             311..1546
                     /locus_tag="VI935_09590"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HLE25885.1"
                     /translation="MKIVIFQGDGMPDHPIPELGGKTPLEAANTPNMDELAKRSVFGL
                     VKTIPDGLPPGSDVGNLSVLGYNPKLYYTGRSPLEAASIGVNVRETDVTFRCNLVTLK
                     DSNGNTIMEDYSAGHITTEEAREIILDIRKELEDNEVKFYPGVSYRHLMVWRGGSTGL
                     TTGGLDKIKTTPPHDISGKEIASYLPSGEGADKLKDLIDKSKKLLNDHPINHKRIKAG
                     KNPATSIWLWGQGRAPKMPKFKELYGIEGAVISAVDLVKGIGVYAGLQVINVPGATGY
                     LDTNYQGKVEYALRALDEVDLVMIHIEATDETGHEGKVDLKIRAVEDFDSRIIGPVLE
                     GMKRFNDYKILLLSDHPTPIELKTHINEPVPFALFSSKDGSVKDNNRVFSEKSASTTG
                     VFVDEGWKLIGMVLENNYG"
     gene            complement(1636..2406)
                     /gene="mazG"
                     /locus_tag="VI935_09595"
     CDS             complement(1636..2406)
                     /gene="mazG"
                     /locus_tag="VI935_09595"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HLE25886.1"
                     /translation="MKKSFEDLVELSKYLRSPQGCPWDREQTLNTLRTFILEEAHEVI
                     QAIEMNDIEELKEELGDLLYQILFTAQICNEVGKFTVEDVVNQLYYKLVRRHPHVFGE
                     EKAKDAEEAVKRWHGEKLKEKSRKRNLLQIPRSLPSLLRAQRVGEKASQVGFDWEKTQ
                     DVIKKVKEELGELQKAIQTNEQNVIEKEWGDLIFSLVNLARFLKIDAEVSAHKAIDVF
                     IERFQRIEEKAKDIDKTLSGLTLKEMDELWEEVKKEDL"
     gene            2692..3591
                     /locus_tag="VI935_09600"
     CDS             2692..3591
                     /locus_tag="VI935_09600"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HLE25887.1"
                     /translation="MITWIKKGVSHLSTIHILAYFKKIPLLNKLLMPGSGLERMLGKV
                     STPFRNKIRQLIGGIYFETNIYGTKIKMIDYRTYDYYSLFSRGKIHEPALTLQLKTLF
                     EKTNSPTFVDIGAHYGYFTIYAGKLIGSSGQVISIEPHIKFYGCLLKNIEINGLKEIV
                     RTFNLALSDKEGKANMKGWDERVLHEAENGNIQVITFDQLCENEGIRPDIIKIDVHGA
                     EGKVLDGMPDILKNKVSHLFCELHKNMMGYTNSKIIQILENAGLEVFEFKKHREVSGG
                     ELVLISSEFLSNYEDRMLYARRK"
     gene            complement(3645..4595)
                     /locus_tag="VI935_09605"
     CDS             complement(3645..4595)
                     /locus_tag="VI935_09605"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HLE25888.1"
                     /translation="MDTVTHSLIGALGSRAGFSQRVGRVATIAFVVGAVFPDVDIVVS
                     IFGPEFSLRHHRGITHSVIAAPFFAFILGAIINRFSSYKNLRVLALMVALGIYSHIFF
                     DLITSYGTVIFDPLSMKRYSWNLVFILDPFITLPVLAGLIWSWRRKETALRVSIAVFL
                     FLALYLLFCIYSRETSLKRINDFARVKSLDVIKSSVYPRPFAPFLWLGVIETKEDFYS
                     GNLSIFSGVNDLEEIPKSDENSYIKLANKLDEVKLYRWFAEFPVSFYQNENGKHVVGF
                     YDLRFRMLPNRFPFLLKVVFDKNGSVDNVSLNGRTVQQRF"
BASE COUNT         1503 a          879 c          922 g         1336 t
ORIGIN      
        1 gagtggtgag tgatggaagc tctagatcag acaagggagt caggcttcgg gctaaagcaa
       61 cggggctggc tgtcaagaaa tttttcaact gtagccaggt cggtagaggt ttttctcttg
      121 acatcccctt tcatagaact agtaattaga ctgcaaaaat gcagtataat tccctattag
      181 gggtgttata ctgcaaatcg tttaataccc aagttataac aaatgtaatc tcatgtaaag
      241 aggtaatttt aaaaattcct atccagattt ttgttgatat atcttctgaa agcatttaat
      301 cttttcccct atgaaaatcg tgatatttca aggcgatgga atgccagatc accctatacc
      361 tgagcttgga ggtaaaacac ctcttgaggc cgctaacact ccaaacatgg atgaactagc
      421 aaaaaggagc gtttttggat tggtaaaaac catacccgat ggactacctc cagggagcga
      481 tgtaggtaat ctaagcgtgc ttggatacaa tccgaaactc tactacacag gaaggtctcc
      541 gcttgaggca gcaagtatcg gtgtaaacgt tagggagact gatgtcacgt tcagatgcaa
      601 ccttgtcacg cttaaggatt caaatggaaa tacaataatg gaggattata gcgcgggaca
      661 tattacaacc gaagaggcta gggaaattat cctagatata aggaaagaat tagaagataa
      721 cgaggtgaaa ttctatcccg gtgtaagcta tcgacacctt atggtatggc gtggtggttc
      781 gacagggctc accacgggcg gtcttgataa aataaaaaca actccgccac acgatatttc
      841 cgggaaagaa attgcatcat acctgccaag tggtgaaggc gcagataagc ttaaagacct
      901 tatagacaaa tccaaaaaac ttttaaatga tcatccgata aatcataaga gaataaaggc
      961 tgggaaaaac ccagctacaa gcatctggct ttggggacag ggaagagcac caaagatgcc
     1021 aaagtttaaa gagctctacg gaattgaagg cgcggtcata tcagcagtgg atttagtaaa
     1081 aggaatcggg gtatatgccg gtcttcaagt aataaatgtc cctggtgcaa caggttatct
     1141 tgatacaaac taccagggaa aggttgaata tgcccttcgt gcattggatg aagttgacct
     1201 cgtgatgatt cacattgagg ctacagacga aacaggacat gagggcaaag tagatttaaa
     1261 gatacgtgcg gttgaggatt ttgatagccg tataataggt ccagtactag aggggatgaa
     1321 gagattcaat gattataaaa ttcttctcct gtcagaccac ccaaccccta ttgaactaaa
     1381 aactcacata aacgaaccag taccctttgc gttgtttagt tcaaaggacg ggagtgtaaa
     1441 ggacaataat agggtgtttt ccgagaaatc agcttccacg acaggtgttt ttgtagatga
     1501 agggtggaaa ctgataggaa tggtattaga gaacaactat ggttaagctt ccaaatcgct
     1561 gtcatgattg taggggcaac ccttgcggtt gcccaaaaga atagggcgaa tgcaagattc
     1621 gcccctaccc tcacactata aatcctcctt ttttacctcc tcccaaagct catccatctc
     1681 tttgagtgta agtccggata atgttttatc tatgtctttt gctttttctt caatcctctg
     1741 aaatctctct ataaatacat caattgcctt gtgcgctgag acctcggcat ctatttttaa
     1801 aaacctcgcc aggttcacta atgagaagat caaatctccc cattcttttt ctatcacatt
     1861 ttgctcatta gtttggattg ctttttgaag ttcccctagt tcttctttta ccttttttat
     1921 aacatcttgg gttttttccc aatcaaagcc aacttgagag gccttttccc ctaccctttg
     1981 cgcacgaaga agtgacggaa gggacctggg gatttgaaga agatttcttt tcctcgattt
     2041 ttctttcaac ttttcaccat gccatctctt aacagcttct tctgcatctt ttgccttttc
     2101 ttctccaaac acatggggat gacgcctgac aagtttgtaa taaagctgat tcaccacatc
     2161 ttctaccgtg aattttccta cctcgttaca tatctgagct gtaaataaaa tttgatataa
     2221 aagatcccca agctcctctt tgagttcttc aatatcattc atttcaatcg cctgaattac
     2281 ctcgtgtgcc tcttctaaga taaaagttct aagtgtatta agtgtctgct ctctgtccca
     2341 agggcatcct tgaggacttc tcaggtactt agatagttcc acgagatctt caaaactctt
     2401 tttcatcctc agctccgatg agtttacaat ttaaagtatg ttaatattga aactagacaa
     2461 ggtcaatcta atatattacg aaccaattaa atttgatcga tgcgaggtct tatcgagaac
     2521 acagagaatg caaggtgtcc attttattca cactagggat acataatcaa aatactcatt
     2581 gagagcgttt ggaaagaaag gaaaactctc tgcaggcatt ttattgagat gagattatta
     2641 tacgtaagat aataacaaga tttaaattta aacatacaat tggataattg aatgataaca
     2701 tggattaaaa aaggagtgtc acatttatcc actatacaca ttttggctta ttttaaaaag
     2761 attcctttat tgaataaatt attaatgccg ggaagtggtt tggaaagaat gttaggcaag
     2821 gtgtcaaccc cttttagaaa taagattaga caacttatag gaggaattta ttttgaaact
     2881 aatatatatg gaacaaagat aaagatgata gactatagga catatgatta ttattctctt
     2941 ttttcacgag gtaaaatcca tgaaccagcc ctaaccctgc agcttaaaac actctttgaa
     3001 aagaccaatt cacccacctt tgtagacata ggagctcatt acggttattt cacgatttac
     3061 gctggtaaac ttataggttc aagcggacag gttatatcaa ttgaaccgca tattaaattc
     3121 tatggttgcc ttctgaaaaa tattgagatc aatggcctta aagaaattgt tcggactttt
     3181 aatctagcac tttctgataa agagggtaag gcaaatatga aaggctggga tgagagggtc
     3241 cttcatgaag cagagaatgg taacatacaa gtaataacat tcgatcaact gtgtgaaaat
     3301 gagggtattc gacccgatat tataaagatc gatgttcacg gtgcagaagg aaaagtgctt
     3361 gacggaatgc ctgacattct taaaaataaa gtttctcact tattctgcga gttgcataaa
     3421 aatatgatgg gctatactaa tagcaagatt atccaaattt tggaaaatgc tggcttagaa
     3481 gtttttgagt tcaaaaaaca tcgtgaggta tctggcggag aactggttct aataagtagc
     3541 gaatttttat caaactacga agatagaatg ctatacgctc gaagaaaatg aaatttattc
     3601 ttagtatgct aaggataata ggtatggtgt gagttgttta taaatcagaa cctttgctgg
     3661 acagttcttc catttagtga aacattatcc acagatccat ttttatcgaa aactactttc
     3721 aagagaaagg gaaatctgtt cggcagcatt ctaaacctca agtcataaaa cccaacaaca
     3781 tgtttcccat tttcattttg gtaaaatgac acaggaaact cagcaaacca gcgataaagt
     3841 ttgacttcgt caagtttgtt tgcaagttta atgtaggaat tttcgtctga ttttggaatc
     3901 tcttcaagat catttacgcc actgaaaatg ctaagatttc cactgtagaa atcctccttg
     3961 gtttcaataa cacccagcca taaaaagggt gcaaacggac gagggtaaac tgaggatttt
     4021 attacatcaa gtgactttac tctagcaaaa tcgttgattc tttttaaact tgtttctctg
     4081 ctatagatgc aaaaaagcag atataacgca agaaaaagga aaacagcaat agaaactctg
     4141 agtgcggttt ccttccttct ccaagaccag atcaatcctg ccagaacagg aagcgttatg
     4201 aatggatcaa gtatgaacac aagattccag ctatatcgtt tcatgctcag tggatcaaag
     4261 attacagttc catatgatgt aataagatcg aagaatatgt gggaatagat cccaagagca
     4321 accatcaagg cgagaactcg gaggttttta tacgaagaaa agcgatttat tatcgcgcca
     4381 aggataaaag caaaaaatgg tgctgcgatt accgaatggg ttatccctct atggtgtctt
     4441 agagaaaact caggaccaaa tattgagaca actatatcga catcaggaaa aaccgcaccg
     4501 acaacaaagg caatcgttgc aacccttcct accctttggg aaaaacccgc cctcgaacct
     4561 aaagccccta taagactgtg agtaactgta tccaattttt ataaatgata cacgctgcat
     4621 gacacatgat ttatgatata
//
LOCUS       DATDRL010000286         3684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2999063, whole genome shotgun sequence.
ACCESSION   DATDRL010000286 DATDRL010000000
VERSION     DATDRL010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2999063"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VI935_09610"
     CDS             complement(<1..1028)
                     /locus_tag="VI935_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HLE25889.1"
                     /translation="MSLKLFLRTKKIEILLFFLLIIGLGLKLYRLDNPNSYYFDEIYY
                     AFTAQEMAKGNRAGWEIGHTAPQGFAYEWSHPHLGKELSAIGILIFGDNTFGWRFFQA
                     LFGGFGTLFIYLLGKELFLSKRAGLIAAFLFTFESFIFVLSRIAMVDIFLMSFILLAS
                     LFLVKYARTPKIAFLLLSGFFCGAAISVKWSGIYALEFLAASSFLLIFYREAYSEIEK
                     KGSFYFQFVKIIPKIFLVFVVIPFVVYLATYIPFFLYGNSFSDFISLQNNMYGYHKGL
                     TATHPYQSSWWKWPLLLRPVYLYLGSGKEESAHIYAIGNPFIWWSGCVFLILSVVQVI
                     RKELPALA"
     gene            complement(1036..2427)
                     /locus_tag="VI935_09615"
     CDS             complement(1036..2427)
                     /locus_tag="VI935_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25890.1"
                     /translation="MNLGFYKEKEGLKWIYPFFPLLLIVGFILRILFSPSWTYEGDFG
                     SWKGWAYGISKVGFSEFYDEYWCDYMPGYLYILWLLQHLHTAFPMIPDTILFKLPANL
                     SDLGISIVIFYVLRNITNVRNAKIASLAYFFNPASLSNSTFWGQVDSVHTLPLLVSIF
                     LGVRRHFVISIVFAVIAFMIKPQSIVIFPIIGFFVIRDVIRRKKEDRLFLKTFILGVK
                     IFATSIITIIVITLPFIWDEIAKDSLTGIIIEPIFFIKERFFMAYNQYKYTSLNAFNF
                     WAMIHGMWISDQDMFLDITYKRWGTIIFGAFYALIFGFLFLSEVMRRSEAGFKNFEYI
                     KSENAYGMTADGLLTFRTFHVVTLILFALFLFVTRAHERHFLPTIVFFTMIVFRSRLY
                     WLFYALISLVYVINMAYAYIERYPIHSFSSSIIEVCIPGMVIMLLFVFLVVLADFIKN
                     SLSLYKGEVIKER"
     gene            complement(2408..3112)
                     /locus_tag="VI935_09620"
     CDS             complement(2408..3112)
                     /locus_tag="VI935_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLE25891.1"
                     /translation="MKKDISLSVVLPAYNEAKNIEGTVSEAISYLRARFERFEIIVVN
                     DGSVDGTREVVERLSLSNPMVVLINHPVNLGYGSALRSGFERASLDYIFFMDSDGQFK
                     ISDVDRLIPFIENYDVIVGYRGKRVDPFIRSLNTWLYHLFIRFLFGLKIKDMDCAFKL
                     FPRTAYLAIKPIKSGGALISAELLIKLGKKGFKIKEVPVRHFPRRFGKQTGANLKVIL
                     RMFKECWKLRNELRVL"
     gene            complement(3192..3425)
                     /locus_tag="VI935_09625"
     CDS             complement(3192..3425)
                     /locus_tag="VI935_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HLE25892.1"
                     /translation="MIRLPIVNFKTMEKVLLRLGFEAVRQKGSHVFYRHPDGRTTTLP
                     NHPGMDLARPLIREILREIELTPEQFREELEKI"
     gene            complement(3422..3643)
                     /locus_tag="VI935_09630"
     CDS             complement(3422..3643)
                     /locus_tag="VI935_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLE25893.1"
                     /translation="MRTFTAYVEFDPETKLYVGIVPGIPGAHTQGSTLDELQKNLKEV
                     LELCLEEYKGSKEDLPRFVGLQQIEVAYV"
BASE COUNT         1273 a          761 c          650 g         1000 t
ORIGIN      
        1 aaagcaagcg ctggtagctc cttacgtatt acttgtacaa cgctcaaaat tagaaacacg
       61 catccagacc accaaataaa tgggtttcct atcgcataaa tatgggcact ttcctcctta
      121 ccgcttccga gatataaata aacaggtcta agaagtagtg gccatttcca ccaagaagac
      181 tgataaggat gtgttgccgt aagcccttta tggtaaccat acatgttatt ttgaagagat
      241 ataaaatcac taaaagaatt tccataaaga aaaaaaggaa tatatgttgc aagatagacc
      301 acaaatggaa tgaccacgaa tacgaggaat atcttaggaa tgatttttac aaattgaaaa
      361 tagaaggagc cctttttttc aatttctgaa taagcctcgc gataaaatat taacaaaaac
      421 gaagaagcag ctaaaaattc aagggcataa ataccactcc attttactga tatagctgcc
      481 ccacaaaaaa agccagaaag caatagaaaa gcaatttttg gggttcttgc gtacttcaca
      541 agaaaaagtg atgcaagtag aataaagctc ataagaaaaa tatccaccat tgcgatacga
      601 cttaacacga agataaacga ttcaaatgta aataaaaaag cggcaataag cccagccctt
      661 ttacttagaa aaagctcttt gccaagcaga taaataaata aagtcccaaa tcctccaaaa
      721 agagcctgga aaaaacgcca tccaaatgtg ttgtcgccaa aaatcaagat gcctatggcg
      781 cttagttcct ttccaagatg gggatgcgac cattcatagg caaatccttg gggggcagta
      841 tgacctatct cccaccccgc cctatttcct tttgccatct cctgagcagt aaaggcataa
      901 tagatctcat caaaatagta gctatttgga ttgtcaagac gatataattt gaggccgaga
      961 cctattatga gcaaaaagaa aagcaggatt tcaatctttt ttgttcttaa aaacaattta
     1021 agtgacatat tcccactacc tttctttaat cacttctccc ttgtaaagac ttaatgaatt
     1081 ttttataaaa tctgcaagga caacaagaaa aacaaataac aacataataa ccattccagg
     1141 tatacatacc tcaataatag atgaggaaaa actatgaatc ggataccgtt caatatatgc
     1201 ataagccata ttaattacat aaacaagtga gataagagcg tagaataacc agtataatct
     1261 cgagcgaaag acaatcattg taaagaagac gatcgtagga aggaagtgcc tttcatgggc
     1321 gcgggtaaca aaaagaaaaa gagcaaagag tattaaggtt acgacatgaa aagtgcgaaa
     1381 agtgagtagt ccgtcagcag tcatgccgta tgcgttttct gattttatgt attcaaaatt
     1441 tttgaaccct gcctcacttc gcctcattac ctcagaaaga aagagaaatc caaatatcaa
     1501 ggcataaaat gccccgaata taatagttcc ccacctcttg tatgtgatat caagaaacat
     1561 atcctgatca cttatccaca tgccatgaat catagcccag aaattaaaag cgttaagaga
     1621 cgtatatttg tattgattgt acgccataaa gaatctctct ttaatgaaaa agatgggttc
     1681 tataataatg cccgtcagac tatccttagc tatttcgtcc caaataaaag gaagtgtaat
     1741 aacgataatt gtaataatcg aagtagcaaa aatcttaact ccaaggataa acgtttttaa
     1801 gaacaatcta tcttcctttt ttcttctaat aacatccctg ataacaaaaa atccgatgat
     1861 tggaaaaatc acaatgctct gtggctttat catgaaagca ataactgcaa atacaattga
     1921 tataacaaaa tgtctcctga ctcctaggaa aattgaaacg agaagcggaa gtgtatgaac
     1981 agaatcgacc tgtccccaga atgtagagtt tgaaagagag gcgggattga aaaaataggc
     2041 aagtgatgcg atcttagcat ttcttacatt agtaatattt ctaagaacat aaaagatcac
     2101 aattgaaatc ccaaggtctg agagatttgc agggagttta aaaaggattg tatccgggat
     2161 cattgggaaa gcagtatgta agtgttgaag cagccatagg atataaagat acccaggcat
     2221 gtaatcacac cagtattcat catagaactc tgaaaatcca accttagaaa tgccataagc
     2281 ccatcccttc catgatccaa agtctccctc gtaggtccag gaaggagaga aaagaatcct
     2341 taaaataaac ccaacgataa gaagcagagg gaaaaacggg tatatccatt ttagaccttc
     2401 tttctcttta taaaacccta agttcatttc tcagtttcca gcactccttg aacattctca
     2461 atattacttt gagatttgcg cctgtttgct ttccaaaccg acgaggaaag tgcctaactg
     2521 gaacctcttt aattttaaac cccttctttc ctaatttaat aagcaactct gcactaataa
     2581 gtgctccgcc tgattttatg ggtttgatag caaggtaggc tgttctagga aacagtttaa
     2641 acgcacagtc catatctttt atcttcaaac caaagagaaa ccttataaaa agatggtata
     2701 accaagtgtt tagtgaacga ataaacggat ccaccctttt tcccctatag ccaactatga
     2761 cgtcataatt ctctatgaaa ggaataagac gatcaacgtc acttatttta aactgaccat
     2821 cgctatccat aaagaatata taatccagag atgctctttc aaatccgctc cgaagagcag
     2881 agccatagcc caagtttact ggatgattta ttaaaactac cattggattt gataaagata
     2941 gcctttctac aacctctcga gttccatcaa ccgagccatc attaacaact atgatctcaa
     3001 atcgctcaaa tcttgcccta agataggaaa tcgcttcact aaccgttcct tcaatatttt
     3061 ttgcttcgtt ataagctgga agaactacgg agagagaaat atcttttttc ataagctttc
     3121 attttaacat aacctaagtc tgttgccgtg ttttgtgtgg gtttgatctt agatgacttc
     3181 ccagacagtg cttatatctt ctccaactct tccctaaatt gctctggcgt tagttcaatc
     3241 tcacgaagga tttcacggat caatggacgg gcaaggtcca ttccaggatg attcggcaag
     3301 gtggttgtcc tgccgtcagg atgacggtaa aagacatgac ttcctttctg ccgcacagct
     3361 tcaaaaccaa ggcgaaggag caccttctcc attgtcttga agttaacaat tggaaggcgg
     3421 atcatacata ggctacctca atctgctgaa gtcccacaaa ccggggtagg tcttcctttg
     3481 accccttgta ctcctccaag cataattcca gcacttcttt gaggtttttc tggagctcgt
     3541 ctaaggttga cccctgcgta tgggcacccg gaatgcccgg gacaattccc acataaagtt
     3601 tggtctcagg gtcaaattca acataagcag taaaggttct catattaaac ctccttctag
     3661 gattgcctca ctagaataca ttga
//
LOCUS       DATDRL010000287        12880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3527584, whole genome shotgun sequence.
ACCESSION   DATDRL010000287 DATDRL010000000
VERSION     DATDRL010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12880
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3527584"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="VI935_09635"
     CDS             <1..1012
                     /locus_tag="VI935_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HLE25894.1"
                     /translation="GEGGTLVNKDGYRYLQDYDLGKPLEVTDPRHPVKKTMELGPRDR
                     LSQAFMKEVEKGRTLSGEYGEYVHLDLRHLGEKKVDKKIPFVRELSTKYAGIDPVYEP
                     IPVRPVVHYMMGGVDTDIDGATSIPGLFAAGEAACVSINGANRLGSNSLTELLVFGAI
                     AGRIAARFATEHSELDLTALQKQGADEQERIAKNFIRKDGGYERVATLRQEMTGTMES
                     GAGIYCSETSLKETCNKLAELRDRFSNIMIEDRSLTFNTELTSALELDFMLDVAQAVA
                     HSALTRTESRGSHQRTDYPNRNDEKFLKHSLAFRTDGAPRIEYKDVIITRWPPAERVY
                     GR"
     gene            1079..1834
                     /locus_tag="VI935_09640"
     CDS             1079..1834
                     /locus_tag="VI935_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013807755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HLE25895.1"
                     /translation="MEKTMKLEIFRYRPDQEDQPRFQTYEVPFREDWVVLDAINYIKD
                     QVDGTLSYRWSCRMGVCGSCGMMVKGVPKLTCATFLKDYYPDKIRVEPLTGFPVERDL
                     VIVMDDFINKLKAIKPWIIRREGEEKPIEEGEYLQTPAELAEYKQYSMCINCMLCYAA
                     CPVYALDNRFVGPAAIALAQRYNMDSRDQGRDQRQEIIASHEGIWECTFVGECSEVCP
                     KAVDPAGAIQRSKVSSTVDWYKEVLIPWGGKKR"
     gene            1813..2214
                     /locus_tag="VI935_09645"
     CDS             1813..2214
                     /locus_tag="VI935_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HLE25896.1"
                     /translation="MGRKEEMKPYRRPVPNTWWLKRRPYFLFIVRELTSVFVAAYSVF
                     LLLVIYKLGQGPQAYEGMIELLRSPAIIILHLVVLAFAVYHSITWFNLTPKILVLFVG
                     EVRVPPLLIAGAHYFAWLVLSALVAWAILRA"
     gene            2217..2567
                     /gene="frdD"
                     /locus_tag="VI935_09650"
     CDS             2217..2567
                     /gene="frdD"
                     /locus_tag="VI935_09650"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HLE25897.1"
                     /translation="MSKSNEPIWWSLFAAGGVVSAFFVPILIIIIGIVLPSGLVGDEP
                     FVYERIHGAVSHPVTKLILFFLIALTFFIWAHRFLFTLIHMGLHKARSVISVLCYGSA
                     IVGTILTAIILWQI"
     gene            2716..3870
                     /gene="sucC"
                     /locus_tag="VI935_09655"
     CDS             2716..3870
                     /gene="sucC"
                     /locus_tag="VI935_09655"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011065943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HLE25898.1"
                     /translation="MNIHEYQAKELLAKYVPAGKVAFTPEEAHQIAQDLRGKKLVVKA
                     QIHAGGRGKGGGIKLANSPDEVRRIASEMLGMRLVTHQTGVEGKIVRKVLIEEASDIE
                     RELYLGVVIDRAKERIVLMASSEGGVEIEEIARTRPKTILKEYVDPALGLFPFQCRKI
                     AYFLGLDGKTANKAVNFISGLYNAFVDNDCSLAEINPLVITREGDVLALDVKLNFDDN
                     ALFRHPEIEKLRDPEEEDPTELEAKKWGLSYVKLNGNIGCLVNGAGLAMATMDIIKYY
                     GSEPANFLDVGGGASTEQVTQAFKMILRDQNVKAVFVNIFGGIMKCDTIATGIITAAK
                     EVGIKVPIVVRLEGTNVELGRKMLAESGLNITTGADMREAAGKAVAAAGK"
     gene            4187..4378
                     /locus_tag="VI935_09660"
     CDS             4187..4378
                     /locus_tag="VI935_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25899.1"
                     /translation="MNLRKKQGFNLWQKSFYDHILRNEESVIETVRYIFNNPVRKGMV
                     GEFKEYPFFGSMVFDIEDI"
     gene            4617..5480
                     /locus_tag="VI935_09665"
     CDS             4617..5480
                     /locus_tag="VI935_09665"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HLE25900.1"
                     /translation="MREIAFRDPLSLFLKEIEKYPVLSREEEYELALKHYKEGDLEAA
                     RRLVVSNLRFVVKIASQYASYGFPLLDLIQEGTIGLMRAVKKFNPYRGYRLISYAVWW
                     IKARIQSYIMKSWSLVKIGTTQAQRKLFQSLRKAKRELSISHDELTNEDLKNIAGFFD
                     VREKDVLGMEMRMAGRDFSLNMASSDDESITYIDSIPDSRENQEDIIDSVETNEMIKE
                     SMQDGFQKLSQKERYIVEKRYLSNPPLKLKDLGGELGISKERVRQIESQALKKLRMVV
                     ENRLNGGYAIT"
     gene            5632..7557
                     /gene="dnaK"
                     /locus_tag="VI935_09670"
     CDS             5632..7557
                     /gene="dnaK"
                     /locus_tag="VI935_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HLE25901.1"
                     /translation="MASKKIIGIDLGTTNSVVAIVEGGEPKVLINEEGSRLTPSVVGF
                     TKDGEILVGGPAKRQAVVNPENTIYSIKRLMGRRYEEATDEAKRLPYKVVKASVGDAW
                     VEAMGKQYSPPQISAYVLMKLKKAAETYLGEEVSEAVITVPAYFNDSQRQATKDAGKI
                     AGLNVRRIINEPTAAALAYGFDKRKEGTIAVYDLGGGTFDISILEVGDNVIEVKSTNG
                     NTHLGGDDFDRRVMDYLISEFKKETGIDLGRDKIALQRLREASEKAKCELSSTLETEF
                     NLPFITADAAGPRHLIMKLTRSKLEQLIEDLIIGTLEPCRQALKDAGLKSSDINEIIL
                     VGGSIRIPYVQKVVSDFFGKEPHKGINPDEVVAVGAAIQGAVLGGEVRDVLLLDVTPL
                     SLGIETLGGVMTSIIGRNTTIPTKKSQIFTTADDSQTSVEIHVLQGERPMARDNRTLA
                     RFILDGIPPAPRGIPQVEVGFDIDADGILHVGAKDLATQKEQKMRVEASSGLSKEQVE
                     KMVKDAEEQTEEDKKRREFVENRNRLDDLIYRTDKSFKELKDKLSEGDRKELEDALEE
                     GRKVLKSEDINEIQTAMERITKASHKIAEVAYKQASGTGAGAGTGTYGAGEGTEGGEA
                     GKKKGGDDVIDAEFTEK"
     gene            8193..8405
                     /gene="rpmE"
                     /locus_tag="VI935_09675"
     CDS             8193..8405
                     /gene="rpmE"
                     /locus_tag="VI935_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HLE25902.1"
                     /translation="MKAGIHPEYKEVVVTCACGATYKTRSTRSGNFTVEICSNCHPFY
                     TGKERTAEAKGRVERFRRRYGEKEAS"
     gene            8644..10725
                     /gene="fusA"
                     /locus_tag="VI935_09680"
     CDS             8644..10725
                     /gene="fusA"
                     /locus_tag="VI935_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HLE25903.1"
                     /translation="MKEYPRRDVRNVAIIAHHGAGKTSLAEAMLFLSGATQKLGKVDD
                     GSSILDYELEEQNRRMSINSHVAFYEWNDHLVNLVDTPGFANFLFEAESALRVVDGAI
                     AIVSAITGVKAQTQKFWQLAADLGIPRVLFMNKLDRERASFENCIADIEKDLGIRPVP
                     ITIPIGEEENFKGVVDVIEMKAHIFGEGGKYAIESIPKDLESQANVWRERLVESVAEL
                     NDELLEKYLGGGTFDNALIYKMLREGVLSAKIAPVYVGSAVKLIGINLLMDGVCRNLP
                     SPIERPSIKGHNTKGEEVEKEPKVEESMSAYVFKTISDPYAGKISIMRIFSGSIKADS
                     TIFNTVKKSREKIGQLFRISGKKQLPVSPALFGDIVTVNKLKDTETGDTLSDEANPII
                     IPHQGMPQAVISYAIQPKTRADEDKLMSSLSRIREEDPTVLSRRDEETKEFLLSGTGQ
                     VHVEITVDKLKNKYGVNVEMKTPKVPYKETIKAIAKAEGKYIKQTGGRGQYGDAWLQV
                     EPLRSGGYEFVDRIVGGVIPKNFIPSVEKGVKEAMHKGILAGYPVVDVKVTLFDGKYH
                     PVDSSDIAFKIAGSMGFKKAMEMAQPVLLEPIMRVESISPEDCLGDVIGDINARRGKV
                     SGVEPQAGGHVVKALVPMAEILTYAPELRSITSGRGMFSMEFSHYEEVPSHLASKIIE
                     GASKEKQAAEE"
     gene            10839..11363
                     /locus_tag="VI935_09685"
     CDS             10839..11363
                     /locus_tag="VI935_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HLE25904.1"
                     /translation="MIKPYNGIFPTIHSSAFIADSAEVIGDVTIGEESSVWFQTVIRG
                     DVNFIKIGARTNIQDNSVLHVTHDTHPVIIGNDVTAGHRVVIHGCNISDCVLIGIGAI
                     VLDGAIVEENSIVGAGSLVAPGFKVPSGTVVMGVPAKVKRELSPEEVMQIKQSAANYI
                     EYSKKHKLVGGSGQ"
     gene            11689..12606
                     /locus_tag="VI935_09690"
     CDS             11689..12606
                     /locus_tag="VI935_09690"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HLE25905.1"
                     /translation="MRIASIDIGTNTFRILVGEIRDKEIKKLYINRVITRLGGGFSKE
                     GKITEDAAKRSLSALKKFAKIIERYKVKRVRAVATSVVRESQNGSKFLEQVKKETGIK
                     TEVISGEEEAQLTVNGVLKSVSVDTKYSVIFDVGGGSTEYVFVKKGQVLGLTSTNLGI
                     VYLTERYLKADIPSSSDIKKLSKEIEKVLSVKLCWIPTVLPSKLTLIGTAGTPTTLAA
                     IQLGLKKYDPDLVNGFILKHDMLTSTFESLIRIPPRDRLKIPALEKGREDVIIPGALI
                     VLKTMDRFSKDSVLVSDGGLLEGVAYSLL"
BASE COUNT         3863 a         2403 c         3267 g         3347 t
ORIGIN      
        1 cggagaaggg ggcaccttag ttaacaagga tggttaccgc tatctgcaag attacgattt
       61 gggtaagccg cttgaggtta ccgatccgcg ccatccagta aagaagacaa tggaacttgg
      121 cccccgtgat cgcttaagcc aagccttcat gaaagaggta gagaagggtc gcacgctatc
      181 aggggaatat ggagagtatg ttcacctcga tctcaggcat ctcggtgaga aaaaggttga
      241 taagaagata ccgtttgtgc gggagctctc aacaaagtat gctgggattg accctgtcta
      301 cgagcctatt ccagttcggc cagtcgtcca ctacatgatg ggcggggttg acactgatat
      361 agacggggca acttcgatac cgggtctctt tgctgcagga gaggcagctt gtgtctcgat
      421 caacggagca aatcggcttg gctccaattc gctgaccgag cttttggtct tcggagcaat
      481 agctggacgg atagcagcaa ggtttgcaac tgaacattcc gaacttgatc tcactgccct
      541 ccagaaacag ggagccgacg aacaggagag gattgcaaag aactttattc gtaaggatgg
      601 aggctatgaa cgcgtagcca cccttcggca ggagatgacg ggaaccatgg agtctggtgc
      661 cggtatttac tgctctgaga caagccttaa agaaacctgt aataagctgg ctgaactcag
      721 agatcgtttt tctaatatca tgattgagga caggagcctt acctttaata ctgaacttac
      781 ctcagccctg gagcttgact tcatgctcga cgttgctcag gcggtagcgc atagtgcgct
      841 taccagaact gagagtcgcg gctcgcatca acgaaccgat tacccgaatc gcaacgacga
      901 aaaatttctc aagcactcgc ttgcctttcg cactgatggc gcgccccgga tcgaatacaa
      961 ggatgttatc atcacacgct ggcccccggc tgagcgtgta tatggaaggt aaattccaat
     1021 tgaatgtata gagacgtgtt gcaatgcgtc tctactttat ttttaatcgg agcagaaaat
     1081 ggaaaagacg atgaaattgg agatattcag gtaccgtccg gaccaggagg atcaaccgag
     1141 gtttcaaacg tacgaagtcc cctttaggga ggactgggta gttcttgatg cgatcaacta
     1201 cattaaggat caagttgatg gaacgctctc ctatcgttgg tcctgccgta tgggggtttg
     1261 tgggagttgt ggaatgatgg taaagggtgt tccgaagctc acgtgcgcga ctttcctcaa
     1321 ggactactat ccggacaaga ttcgggtaga gccgctcaca gggtttcccg tagagcgaga
     1381 ccttgtaata gttatggatg acttcatcaa taaactcaaa gcgataaagc cgtggattat
     1441 ccgccgggag ggtgaagaga agccgatcga agaaggggaa tacctccaga cccctgctga
     1501 actcgcggag tacaagcaat actcgatgtg cataaactgc atgctttgct atgcggcgtg
     1561 tcctgtatat gctcttgaca acaggttcgt tggtccggca gccattgcgc tcgcacaacg
     1621 ctataacatg gactcccgag accagggacg ggaccagcgt caggaaatca tcgcgagcca
     1681 tgaaggcatt tgggagtgca ccttcgtcgg cgagtgcagc gaagtctgtc ctaaggccgt
     1741 agaccctgcg ggagctattc agaggtcaaa ggtatcgagc actgttgact ggtataagga
     1801 agtgctaata ccatggggag gaaagaagag atgaaacctt atcgccgacc tgttccaaac
     1861 acgtggtggc ttaagcggcg cccctacttc ctttttatag tccgtgagct gacctcggtc
     1921 tttgtggcgg cgtatagtgt ctttttgttg ctggtcatct acaagcttgg ccaaggacct
     1981 caggcttatg aagggatgat tgaacttctc aggtcgcctg cgattataat attacactta
     2041 gtcgttcttg cgttcgcggt ttatcacagc attacctggt tcaaccttac tccgaagatc
     2101 cttgttctgt ttgttggaga agtgcgagtc ccgccactcc ttatcgccgg agcccattac
     2161 ttcgcttggc tggttctttc ggccctagtg gcctgggcca tattgagggc ataactatga
     2221 gcaaatcaaa cgaaccaatc tggtggtctt tgtttgcggc gggaggcgtt gtttctgcgt
     2281 tcttcgtccc aattctgata attataatag gcatcgtctt accatctggc ttggttggag
     2341 acgagccatt tgtctacgag cggatacatg gtgccgtttc tcatccggtg acaaagctta
     2401 ttcttttctt tctcattgcg cttacgttct ttatctgggc tcaccgcttt cttttcacgc
     2461 tcattcacat ggggctacac aaggcacggt ctgtcatctc agtcctttgc tatggcagtg
     2521 cgatagttgg cacaatcctc acagctatca tactctggca gatttaagca gtatgcaaga
     2581 tacaggatgt agaatacaga atgtaagtat aacctttatg aatcactccc catgaataaa
     2641 gcatctcgca tcttttatta gtgcaaatcc gtgttcattc gtggcaaaat agtagcatct
     2701 taggaggaaa ctgctttgaa tatccatgaa tatcaggcga aagagttact ggcaaagtat
     2761 gtcccggcag gaaaggttgc gtttacaccc gaagaagccc atcagatagc acaagatttg
     2821 agaggcaaaa aacttgttgt aaaagctcag attcatgcgg gtggaagagg caagggagga
     2881 ggaataaagc ttgccaatag ccctgatgag gtaagacgaa tcgcttcgga gatgcttggt
     2941 atgcgtcttg tcactcatca aacaggtgtg gaaggaaaga tcgttagaaa ggtactcata
     3001 gaagaggcct cggatattga aagagagctt tatctaggag ttgtgattga tagagccaaa
     3061 gaaagaatcg ttttaatggc aagctcagaa ggtggggttg aaattgaaga aattgctagg
     3121 acccgcccga aaacaatcct caaggaatat gtcgatcctg ccttggggct ttttcccttt
     3181 cagtgtagaa agattgcata ctttttgggg cttgatggaa aaactgcaaa taaggcagtg
     3241 aattttattt ctggtcttta taatgcattc gtagataatg actgttcgtt agcagagata
     3301 aatccacttg ttataactcg tgaaggagat gttctcgctc tggatgtaaa attgaacttc
     3361 gacgataatg cgctttttcg tcaccctgaa atcgaaaaac tccgtgatcc ggaggaggaa
     3421 gacccgacag agcttgaggc gaagaagtgg ggtcttagct acgtaaaact caatggaaat
     3481 atcggatgcc ttgtaaatgg agcgggtctc gcgatggcga ctatggatat tattaagtat
     3541 tacgggagcg agccagcaaa cttcctggat gttggcgggg gcgctagcac agagcaggta
     3601 acccaggcat tcaagatgat acttcgcgat caaaatgtga aagctgtgtt tgtgaatatt
     3661 ttcggcggca ttatgaagtg tgacaccatt gctacaggaa ttataacagc ggctaaagag
     3721 gtggggataa aagtaccaat tgttgtaagg cttgagggca ctaacgtaga actaggaaga
     3781 aagatgcttg ccgagtcggg cttaaatatc acaactggcg ccgacatgcg tgaagcagcc
     3841 ggaaaggccg tagctgcggc tggaaaatag cgacaacctt taggtctcca cacagtagat
     3901 tgtaggcagg aatttattcg tgcgattctg cgaacctcaa tccgaggtta gaaaacctcg
     3961 gctatagatt ttatcaccat ctgcacctac aacaaaaaga aaatctttga caacactaag
     4021 accatatcag aaatggtcaa ttatctcaaa gaaactgcca acaagctagg ctttcaaata
     4081 ctcgcctcct gttttatgcc agaccatgtt cacattttaa taggtgcaga tggtgcaaat
     4141 aatgacctaa tcaagtttaa agcacatttt aaactgaaga cgggttatga atttaagaaa
     4201 aaagcaaggt tttaatttat ggcaaaaaag cttttatgac catatacttc gcaacgaaga
     4261 gtcggttata gagacggtaa gatacatatt caacaacccc gttagaaaag gtatggttgg
     4321 ggagtttaaa gaatatccat tctttggttc aatggttttt gatatagagg atatttgaaa
     4381 cgtagcggaa acctttgggt ttccataaca ttggagggct aaagccctcc gctaaatttt
     4441 cacagaggtt aatgtagcga tgagcttcac agatcgctat ctagaaataa tatggaaggc
     4501 taaggccttc cgctacgtga atatgtttta acttcccaaa aacacacaaa atcttatctt
     4561 ttaaaccttg acattccccc tttgatgatt aggtttaggt aatagaggtt aattacgtga
     4621 gggaaattgc gtttcgtgat ccgttaagtc tgtttcttaa agaaattgag aaatatccgg
     4681 tgcttagcag agaagaggag tacgaacttg ctctaaagca ttataaggaa ggagatcttg
     4741 aggcagcgag gaggctcgtt gtttcaaatc ttagatttgt tgttaaaata gcctctcaat
     4801 atgcatctta cggatttcca ctactagatt taatacaaga gggcacaatc ggcttaatga
     4861 gggctgtgaa gaagtttaac ccttaccggg gctacagact gatctcctat gcggtttggt
     4921 ggattaaggc gagaattcaa agctatatta tgaagtcatg gagtcttgta aaaattggaa
     4981 caacgcaggc tcagaggaag ctttttcaaa gccttagaaa ggcaaagagg gagcttagca
     5041 tttcacatga tgaattaaca aatgaagatt taaaaaatat agctggtttt tttgatgtaa
     5101 gggaaaagga cgttcttggt atggagatga ggatggcagg cagggatttt tcccttaaca
     5161 tggcatcaag cgatgatgag agtattacat acattgattc tatacctgac tctagagaaa
     5221 accaggaaga tataattgac tcggttgaaa caaacgaaat gataaaggaa agcatgcagg
     5281 acggttttca aaaactttct caaaaagagc gatacatcgt tgagaagagg tatctttcta
     5341 atccaccgct taagctcaag gatcttggtg gcgagcttgg aatttctaaa gaaagggtgc
     5401 ggcagataga atctcaagca ctaaagaagc ttcgtatggt tgtagaaaat cgtttgaatg
     5461 gtggttatgc tattacttaa aaggtctgcc actaagacac caaggcacaa gggtttaagg
     5521 tctaatagtt caagagttaa acgttaaacc tttgaacttg taaccattga acttttctta
     5581 ccttcgggtc tatgtggcta aaaatttaca taaaatttag gaggggatta tatggcaagc
     5641 aagaaaataa tagggattga cctcgggact acgaattcag ttgttgcaat agtagagggc
     5701 ggtgaaccaa aggttcttat aaacgaggag ggttcaaggc ttacgccttc ggttgtcggt
     5761 tttactaaag atggcgagat ccttgttgga ggaccggcta aaaggcaggc tgtcgtaaac
     5821 ccggaaaata ccatttattc aattaaaagg cttatgggta ggcgttatga agaggcgact
     5881 gatgaagcaa agcgacttcc ttataaagta gtgaaggcct ccgttggcga tgcatgggtt
     5941 gaggcaatgg gaaagcagta ttctcctcct cagatctcag catatgtgct gatgaaacta
     6001 aagaaggctg ctgagactta cttgggagag gaggtatcgg aagcagtaat aaccgttcct
     6061 gcttatttta atgatagcca gagacaggct acaaaagatg cgggaaagat cgcaggctta
     6121 aatgttagga gaatcatcaa tgagccaaca gcagcggctc tcgcttatgg ttttgataaa
     6181 agaaaggaag ggactattgc agtgtatgac cttggtggtg gaacgttcga tatctctatc
     6241 ctggaggttg gagataatgt aatcgaggta aaatccacaa atggcaacac ccacttgggt
     6301 ggggatgatt ttgataggcg tgttatggat tatctaatca gtgagttcaa gaaggagact
     6361 ggaattgatt taggtcgaga taagatagcg cttcaaaggc ttcgtgaggc ttcagagaag
     6421 gcgaagtgtg agctttcgtc aaccctcgaa acagagttta atcttccttt catcactgca
     6481 gatgcggccg gcccacgaca tctaattatg aaactcacac gatcaaagct tgagcagctc
     6541 atagaagacc tgataatagg cacactagag ccctgtcgtc aagcccttaa ggatgcgggt
     6601 ttaaagtcga gtgacataaa cgagattatt cttgttggag gatctattag aatcccctac
     6661 gttcagaagg tcgtaagcga cttctttggg aaagagcctc ataagggaat aaaccctgat
     6721 gaggttgtcg ctgtaggcgc agcgattcag ggcgctgtgc tcggcggaga ggtaagggat
     6781 gttcttcttc ttgatgtaac gccgctttcg ctggggattg agacattggg cggtgttatg
     6841 acgagtataa tcggaagaaa taccactata cctacaaaaa agagtcagat atttaccacc
     6901 gctgacgata gtcagacatc ggttgagatt catgtgcttc aaggtgagcg tccaatggct
     6961 cgtgataaca ggacccttgc tagatttatc cttgatggaa ttccacccgc accgagagga
     7021 ataccacaag tagaggttgg gttcgatatt gatgctgatg gcatccttca tgtaggtgca
     7081 aaggaccttg ctacacaaaa ggagcagaaa atgagggttg aggcatcaag cggtttaagc
     7141 aaggagcagg ttgaaaagat ggtaaaggac gcggaagagc aaaccgagga ggataagaag
     7201 cgtcgtgaat ttgttgagaa tagaaataga ctcgacgact tgatttatag aacggataag
     7261 agctttaaag agcttaagga taaactctct gagggtgata ggaaagagct agaagacgcc
     7321 cttgaagagg gtaggaaggt tcttaaaagt gaggatataa acgagatcca aacggcgatg
     7381 gagagaatta ccaaggcgtc ccataagata gcagaggttg cctacaaaca ggcctccggt
     7441 actggagcgg gagccggaac aggaacatac ggagctgggg agggaactga gggaggcgaa
     7501 gcaggaaaga aaaagggcgg cgatgacgtt atagatgctg aatttacaga aaagtaaaaa
     7561 gtatattata aaagctgttg tttgtttgtc acaaggtagc taatgtaacc ccaattctat
     7621 attaaaacgc agaacaaaat ttagagtcaa tttgtagggg caaaccttgc gttcgcccaa
     7681 tactggcaac cgcgagggca tttgttacaa cataagggat caaaggcaat caacgtggca
     7741 ccccaatgtg aatttgcaag gataggcagg actttctagt cctgcattgg cagacggggg
     7801 ttagaaaacc ccgcctatcc gatgtccttc tgtcctagac tttgctcagt cagcactgct
     7861 agggttgccc ccacccgatg tttattaccg aattagatat tcaaagatca taagctttcc
     7921 tctacatgaa tttaagacct ctaaggttta ggaatctttg aggcttcctt tgagtcgcag
     7981 gagaaatgcg gctcctcccc gaggggcttc aaataaagag gtttgggaat gacaaggcaa
     8041 aatgggtgtt gaagagatgg tgttcctacc tgtcgcggta catttttgtg aggcggaaaa
     8101 ttttgcctct ttactaagaa ttttcattag tctgaataat tgcttgtctc cattttcaat
     8161 gttaaattaa atatccaagc aggaggtatt taatgaaagc gggtatccat ccggagtata
     8221 aagaggtcgt cgtcacgtgt gcctgtggtg caacatataa aaccaggtca acacgctcag
     8281 gaaacttcac cgtagaaatc tgctcgaact gccatccctt ttacacaggt aaagaaagaa
     8341 ccgccgaagc aaagggtagg gttgagagat ttagaaggag atacggagaa aaagaggctt
     8401 cttaggattt ctccttatat attaatttcc caagattcgt gatttgttgt tcgtggttcg
     8461 ttcgactgag ccgtttgtcc ggaggcttcg tcgtgagctt agccgaacgg ctcaagacga
     8521 agtcgaatca cgaaacccgt accactattc acaatccatg cactattcaa taagtaacaa
     8581 caaagcttta caaataagct atcatcgccc tataatatag aaaaatcatg gaggtcacca
     8641 cgtatgaaag agtatccccg tagagatgta agaaacgtag cgataatagc tcatcatggt
     8701 gctggaaaaa cctcgcttgc cgaagcgatg ctttttctca gcggagccac tcagaagctc
     8761 ggaaaggtgg acgatggctc ttccatctta gattacgaat tagaagaaca aaacaggagg
     8821 atgtcaatca attcccatgt tgcgttttat gagtggaacg atcaccttgt gaacctggta
     8881 gacactccgg gatttgctaa ctttttgttt gaggctgaaa gcgcacttag ggttgtagac
     8941 ggtgcgatag cgattgtaag tgctatcacg ggggtaaagg cacagacgca aaaattctgg
     9001 cagttagcgg cggacttagg tatccctagg gtgcttttta tgaacaagtt ggatagagag
     9061 agggcaagtt ttgaaaactg tattgctgat atagaaaagg atttagggat tcggcccgta
     9121 cctattacaa ttcccattgg agaagaagag aattttaaag gtgttgtgga tgtaatagaa
     9181 atgaaggctc atatctttgg agaaggtggg aaatacgcca tcgaaagcat cccaaaggat
     9241 ttagagagtc aggcaaacgt atggagagaa agattggttg agtccgttgc tgagcttaat
     9301 gatgaattac ttgaaaagta tctagggggg ggaacctttg ataacgcatt aatttacaag
     9361 atgctaaggg aaggcgtact aagcgcaaaa atagctcctg tttatgttgg ctctgctgta
     9421 aagctaatcg gaatcaatct gcttatggat ggggtttgcc gtaaccttcc atctccaata
     9481 gagagaccat caattaaggg acataataca aaaggcgaag aggtagagaa agaaccaaag
     9541 gtcgaagaat caatgtcagc ttatgtgttt aaaactattt ctgaccctta tgctgggaag
     9601 ataagtatta tgaggatatt ttctggctca ataaaagccg attcaaccat tttcaacact
     9661 gtaaaaaagt cgcgtgagaa gataggccag ctattcagaa taagtgggaa aaagcaattg
     9721 ccagttagtc ccgctctatt tggcgacatt gttacggtta ataaactcaa ggatactgag
     9781 actggggata ccctttccga tgaagctaat ccaataatta ttcctcatca aggcatgcct
     9841 caagctgtta tatcatatgc gattcaacct aagactaggg cggatgaaga caagctcatg
     9901 tcatcactga gcagaatacg tgaagaagac ccaacagttc tatcaagaag ggatgaggaa
     9961 acaaaagagt ttcttctttc tggaacagga caggttcatg tagagattac tgtagataaa
    10021 ctcaaaaata agtacggagt aaacgtggag atgaaaacac ccaaggtacc atacaaggaa
    10081 acaataaagg caatagctaa ggcggaggga aaatatataa agcagacagg tggtagaggg
    10141 caatatggag atgcttggct tcaggttgaa ccacttcgaa gcggaggata tgagtttgtt
    10201 gataggatag tagggggtgt gataccaaag aactttatcc catcagtaga gaagggcgtt
    10261 aaagaggcta tgcataaggg tattcttgcg ggttatccag tagtagatgt taaggtaacg
    10321 ctctttgatg gaaaatatca cccagtggat tcttcagaca tagcgtttaa gattgccggc
    10381 tccatgggct ttaagaaggc gatggagatg gctcaacctg tgcttcttga acctataatg
    10441 cgcgtcgaat ccatctcacc tgaagactgc ttaggggatg tgataggtga tataaatgca
    10501 agaagaggga aagtatcagg ggtagagcct caagcggggg gccatgtcgt aaaagcctta
    10561 gttcccatgg ctgagattct tacttatgcg cctgagctaa ggtccattac aagcggacgt
    10621 ggtatgtttt ccatggagtt ttctcattat gaggaggttc ctagtcacct tgcttcaaag
    10681 attatcgagg gggcttcaaa ggaaaaacag gcggctgaag aatgaatatc atgaggcgtg
    10741 agacgtaaag tgtgaggggt aagatgattg tctaacgcct tgcgtcttac gccctgcgat
    10801 ttatttttgc cttcgtggct aaatagttag ggatcaaaat gataaagccc tataatggga
    10861 tttttcctac gatacattca agtgcattta ttgcagatag cgccgaggtg ataggagacg
    10921 tcacaatagg tgaggaatct agcgtatggt ttcagaccgt catcagaggc gatgtgaatt
    10981 tcatcaagat tggagctaga actaacattc aagacaatag tgtcttgcat gtcacacatg
    11041 atacccatcc ggttataatc ggaaatgatg ttacagccgg tcacagagtt gtaatacatg
    11101 ggtgcaatat aagtgattgt gttcttatcg ggataggcgc aattgttctt gacggggcaa
    11161 tcgtggaaga gaattcaata gttggtgccg gaagcttggt tgcaccgggc tttaaggttc
    11221 cgtcaggaac cgttgtaatg ggcgttccag caaaggtaaa aagggagcta agtcctgaag
    11281 aggtgatgca aataaagcag tcagcagcaa attatataga atattcaaaa aaacataagt
    11341 tggttggcgg ttcaggacaa tgaaacgtaa acaacgttat tatagatgaa agaagattac
    11401 ggtcacgaac aagactgccc tgagcatact cgaagggact cgtgacctta ccgttggtag
    11461 ttggtaattt gcaattagac atatggatag gggggttttc caaccccaac agacgagaca
    11521 agcctgtcct gagtttaacg aaggaaatgt ctcgtctatc gtgagtacaa taaacgtgga
    11581 agaccaccag gcttgcttgt gtgctggact tacattttat tcatggtcgt tagctattag
    11641 ttgtttgact aatctaaaga ctaatgactg acgactagcg acttaattat gcgtatagcc
    11701 tcaatagata taggtacaaa tacatttagg atactagtag gtgagatcag agataaagag
    11761 ataaaaaagc tctatattaa ccgtgtaatc acaaggctcg ggggcggatt ctctaaagag
    11821 ggaaaaataa ccgaagatgc agctaaaaga tcgctaagtg ccctaaaaaa atttgccaag
    11881 attatcgaac ggtacaaggt aaagagggtg agggctgtag ctacaagtgt ggttagagag
    11941 tctcagaatg gtagtaagtt cttggaacag gttaaaaaag aaaccggaat taagacagag
    12001 gtcatctcag gtgaggaaga ggcacagctt actgtaaatg gtgttttaaa atctgtttct
    12061 gtcgatacaa aatacagtgt gatttttgat gttggtggag gtagcactga atatgtattt
    12121 gtaaaaaagg gtcaggtttt aggtcttaca agtacgaatc tcggtattgt ttatctcaca
    12181 gaaagatatt tgaaggcaga tatcccatct agctcagata ttaaaaagct ttctaaagaa
    12241 atagaaaagg tcttatctgt aaaattatgc tggataccaa ctgtcttacc ttccaagtta
    12301 actttgattg gaactgcagg aacaccaaca acactcgcgg cgattcaact tggtttaaaa
    12361 aaatatgatc cggatttggt taatgggttt atactaaaac atgatatgct caccagtacc
    12421 ttcgaatcct taattaggat tcctccgcga gataggctta agatacctgc actggaaaag
    12481 ggtagagagg acgtgatcat tccaggtgcg ttgattgtgc ttaaaactat ggatagattt
    12541 tctaaagata gtgtgttggt aagtgatggc ggccttctgg aaggtgtggc ttatagttta
    12601 ttgtgatccg gcaatattga ctcgggattc gtggctcgaa tcactaaccc cgattcacgg
    12661 attacggata attcgttgac aatttatgcc atgattatta tatttttcta agactagttg
    12721 acatgaatag agaagagcca tcggaaagag gaaaaagaat acttaattta atagttaatc
    12781 atcatataaa aactggtgag cctatagggt cgaacgcact tgttaaaagt tatgagcttc
    12841 catggagttc agctactgta agaacaacca tggctgatct
//
LOCUS       DATDRL010000288         4115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3264789, whole genome shotgun sequence.
ACCESSION   DATDRL010000288 DATDRL010000000
VERSION     DATDRL010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3264789"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VI935_09695"
     CDS             complement(<1..219)
                     /locus_tag="VI935_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25906.1"
                     /translation="MFKQLFTFGSIINIFILIINLSYAHAISNTADVKDIGVTERLGE
                     YIPLDLTFKDEDGNAVKLSDLFQDEKPVI"
     gene            complement(458..910)
                     /locus_tag="VI935_09700"
     CDS             complement(458..910)
                     /locus_tag="VI935_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HLE25907.1"
                     /translation="MKLNKGFVEHRVENFIIQCRELGIKVTPQRIAIYRELSSTGEHP
                     SAEIIYKKIKKYYPNISLTTIYRTLETFEKLGIISVANILHDAARYDANLNPHHHLVC
                     VVCKRVEDFYDDSLSNLTIPAESLDNYKVLGYSIQLNGICKECQKKKS"
     gene            1118..3151
                     /locus_tag="VI935_09705"
     CDS             1118..3151
                     /locus_tag="VI935_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HLE25908.1"
                     /translation="MKTYFFKNSFMLVGFVVVSLLVLTYISEGEISAENKLPIIREQA
                     KANPKISSLIMKRISKMESLGITRENAKSVYASLISDPLVKVDNSGNIQTYIYMENVD
                     QGNISKLESMNVTIEIVNSKFNLIQAWIPFDIVEEIASLNFVKKITPPSYGILRKGSV
                     NSEGDVVMRSDKVRDELDFDGTGTRVGVISDGVEHIAASQATGDLPNDVIINPTLPGS
                     GDEGTALLEIIHDIAPGAELAFSEGLGTSLTFISSINFLINFAQVDIIVDDIGFLDQP
                     YFQDGMVAQAVEDAIAKGVVYISAAGNNADEHYQALYVDETLGDDPEGINFHDFGVAE
                     GGESDITMPISVFPNNFIVVVLQWNDPFDGSSNDYDLFLFDDEGNLLDRSVDPQTGGS
                     QDHPIEIVSFENNTPDFPTVKVVINRVSGSSRTLEMNFNGRIFVEDFNVPTDGVFGHP
                     AVKDAIAVGAVPATTDKFCSSASGPNEVEDFSSQGPSTIFFTPGGLSILPPERRPKPD
                     VVAPDGILISGAGGFGGQDGIFCGTSASAPHVAGVAALILSKNPNLQPIELGDAIKNT
                     AFPLNLVASLSDNLAETEVFSNISGFGIVDAFKAVQSVPALPTPTPSPTPTPTPTPTP
                     TNSGGDDRGSGGCYVGEKANVSQGLANLLFVLLPIIIVLALKRLKERFFVKSN"
     gene            3207..3434
                     /locus_tag="VI935_09710"
     CDS             3207..3434
                     /locus_tag="VI935_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25909.1"
                     /translation="MKKIGDIQNWNEGDKRDITEKELKTLRDKGLYDSYDKTYVINGF
                     KWKIVSKLVNPDSTVVYTLECVGTEEEQGQV"
     gene            complement(3447..3815)
                     /locus_tag="VI935_09715"
     CDS             complement(3447..3815)
                     /locus_tag="VI935_09715"
                     /inference="COORDINATES: protein motif:HMM:NF045455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25910.1"
                     /translation="MSMWLYDEHGVPRMRLIYDGRILDIEDNLVGWVSLSEGVAYTSD
                     LRHVGWFKNGLIRDNDGWVVAFTRDASDSDMPGLPDPMIPPNISDPISNLDSPNFPKP
                     ALPPEPYALWSAVPLERFFD"
BASE COUNT         1301 a          808 c          805 g         1201 t
ORIGIN      
        1 tattacaggt ttctcgtctt gaaataaatc gctaagtttt actgcattcc catcttcatc
       61 tttaaatgta agatcaagcg gtatgtactc tcctagcctt tcagtaaccc caatatcctt
      121 tacatccgct gtatttgaaa tagcatgggc atatgaaaga ttaattataa gaataaaaat
      181 gttgataata gatccgaaag tgaataattg cttaaacatc aaagaacaga aagtgcctcc
      241 tttcttgatc cataattatc ataagaagcc tcacacgtaa tcctgtacac tctcttaaaa
      301 ttctccatta tttaaaattt tatttgatat ttctgaagat taaaacgaac tatataatct
      361 gcaactttat atgaggttaa tcaattctag tcaaattaaa aaatttctaa agttttagtt
      421 taacggtttc ctaaattctg tcaagaacgg gtttccctta agatttcttt ttttgacatt
      481 ccttgcagat accgtttagc tgaatgctat aacctaggac cttgtagtta tctaaagact
      541 cagcaggaat tgttagattc gaaagcgaat cgtcataaaa gtcctctacc cttttacaaa
      601 ccacacagac taaatgatga tggggattta aattggcatc gtaacgagcg gcgtcatgaa
      661 gtatatttgc tactgatatt attcctagtt tctcaaatgt ttctaatgtt ctataaatcg
      721 tcgtaagcga aatattagga tagtattttt ttatcttctt atatatgatc tcggcgcttg
      781 gatgctcacc tgtacttgaa agctctctat atatagcaat cctctgcggc gtgactttaa
      841 tacctaactc tcggcattgt ataatgaaat tttcaacgcg atgctccaca aaacctttat
      901 tgagtttcat aacatagaaa cctcttggct tctgaaatta cagactacac gaatataaaa
      961 aaatatccaa ttccaaattt taacgaaata tgagactttt tcaataatga atcctgctta
     1021 aaatactaaa acaaaactta atatattatt cacattgtta tgtatactat atagccatat
     1081 tttgtaaaat tgaaagagat atgtgcggag aaataatatg aaaacatatt ttttcaaaaa
     1141 ttcttttatg ttggtggggt tcgttgttgt ttctttattg gtactaactt atatctcaga
     1201 aggagaaatc tcagccgaaa ataagcttcc aatcatacgg gaacaagcaa aggctaatcc
     1261 aaaaataagc tccctaataa tgaagagaat aagcaagatg gaatcactcg gcataactag
     1321 ggaaaacgca aaaagtgttt acgcctcatt gatttcagat cctctggtta aagtagacaa
     1381 ctcaggcaat atccagactt atatctatat ggagaatgtg gatcaaggca acatctctaa
     1441 gctcgaatca atgaatgtaa caattgaaat tgtaaactca aaatttaatc tgatacaggc
     1501 ttggattccg tttgatatag ttgaagaaat agcgagtctc aattttgtaa aaaagattac
     1561 accaccaagc tatgggatat taaggaaagg ctctgtcaac tctgaagggg acgtggtaat
     1621 gcggtctgat aaggtacggg atgaacttga ttttgatgga acagggacaa gggtaggtgt
     1681 aatttcagac ggagttgaac acattgcagc ctcacaagca acgggtgacc ttcctaacga
     1741 tgtaattatt aatcctactc ttcctggaag tggagatgaa ggaaccgcat tacttgaaat
     1801 catacatgac atagctcccg gggcagagct tgcattttca gaaggattgg gtaccagtct
     1861 tacgtttata agctcaatca attttctaat caattttgca caagtagata ttattgttga
     1921 tgacattggt tttctagacc agccatattt ccaagacgga atggtagccc aagcggtcga
     1981 ggatgccatc gcaaagggag tcgtttatat atcggccgct ggaaacaatg cggatgaaca
     2041 ttaccaagca ctttatgttg atgaaactct cggagatgac cccgagggta ttaatttcca
     2101 cgactttggc gtcgccgaag gaggcgaaag cgatattacc atgcccattt ctgtatttcc
     2161 aaataatttc attgttgttg tccttcagtg gaatgacccc tttgacggct cttcaaacga
     2221 ttatgatctt tttctttttg atgatgaagg aaacctgctg gatagaagcg tagacccgca
     2281 aacaggaggt tctcaagatc atcctataga gattgtttcc tttgaaaata atacccctga
     2341 ttttcctacg gtaaaggttg taataaaccg tgtaagtggc tcctctcgaa ccctagagat
     2401 gaatttcaac gggagaatat ttgtagagga tttcaacgtt ccaactgacg gggtattcgg
     2461 acatcctgct gtaaaagatg ctattgctgt aggcgcagtt cctgccacta ctgataagtt
     2521 ctgctcaagt gcatcaggtc ctaatgaggt agaagacttt agttctcagg ggcccagcac
     2581 tatattcttt acccccggcg gactcagtat attaccacct gagcgaagac caaaacctga
     2641 cgttgttgca cccgacggaa ttctcataag cggtgcagga ggttttggag gccaagatgg
     2701 aattttctgt ggaacatcgg catccgctcc tcatgtagct ggtgttgccg cacttatcct
     2761 gagtaagaat ccaaacctgc aaccaattga gctcggagat gcaattaaaa acactgcttt
     2821 tccattaaat ctggttgcga gtttgagtga taatctagca gaaacagagg tattcagtaa
     2881 tatatctggc tttggaatag tcgatgcctt taaagccgtt caatctgtgc cagcactacc
     2941 aaccccaacc ccatcaccaa caccaactcc aacccctact ccaactccaa caaattcagg
     3001 tggtgatgac aggggtagtg gagggtgtta cgtcggggaa aaagccaatg tcagccaggg
     3061 attggctaat ctcttatttg ttcttcttcc catcattatt gttctagcct tgaaaaggtt
     3121 aaaagaaaga ttttttgtaa aatcgaatta gaatatgtgt tttcattgtc accagatagc
     3181 taaacctacg aatcagtgag gaaccagtga agaaaattgg agatattcaa aactggaatg
     3241 agggagataa aagggatata acagaaaagg agcttaaaac tcttcgagac aaagggctat
     3301 acgactcata tgataaaaca tacgtaatta acggttttaa atggaagatc gtatctaaac
     3361 tcgttaaccc tgattcgact gttgtataca cattagaatg tgttggtaca gaggaagagc
     3421 agggacaggt ttgaaacctg cccctgctag tcaaaaaacc tttcaagtgg aactgcggac
     3481 caaagtgcat atggctcagg tggaagagcc ggcttaggaa aattaggaga atctaaattg
     3541 cttataggat cagagatatt aggagggatc atagggtctg gaagccctgg catatcagag
     3601 tcagaggcat ctctagtgaa tgcaactacc catccgtcgt tatcccttat gagaccattc
     3661 ttaaaccagc caacatgcct tagatcagac gtgtatgcca caccctcaga tagagacacc
     3721 catcctacaa gattatcctc tatatcaagt attcgaccgt cataaataag cctcattctg
     3781 ggaacgccgt gctcgtcata tagccacatt gacatcttga atccctccta ctatagtagt
     3841 cattagtcac ttacagtcaa tattaaagca gatcatgaat atatacattt ctatatctta
     3901 catgaataaa cttcttgtta gcctatgaga aggaggtgat cattatgcct ttatatcttg
     3961 tgcagcacac ccacacagcg gaaagttgcc caaccaaaaa ccctgaaatg gtgcggcaac
     4021 tgtctagcca tgtaacacag tctaatgcca gcaagtacgg agttaagatc ttagccgatt
     4081 gggtatatga gccggagcac actgttatcc ttgtt
//
LOCUS       DATDRL010000289         4888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3636274, whole genome shotgun sequence.
ACCESSION   DATDRL010000289 DATDRL010000000
VERSION     DATDRL010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3636274"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /gene="glmM"
                     /locus_tag="VI935_09720"
     CDS             complement(<1..1073)
                     /gene="glmM"
                     /locus_tag="VI935_09720"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HLE25911.1"
                     /translation="MTPEMSLKLGKALTYILKMEKTDSKMPRIVIGKDTRLSGYMFEQ
                     AMSAGIISMGADVLLVGPLPTPAISFLTVSMRADAGVVISASHNPFVDNGIKFFDRNG
                     FKLPDEKELEIEELVLGDRINRLNVSPSDIGKAWRLDDAQGRYVVFVKNTFPKDLTLE
                     GLKIILDCANGAAYKVSPIVFQELGAEVIAIGVRPDGKNINTDCGSINPELLKRKVKD
                     TGADLGIALDGDADRVIFCDEKGEEVDGDIVIAITADEMIRNNKLQKKTLVTTIMSNM
                     SLETFIREKGGIVIRTPVGDRYVVEEMRIRGCNFGGEKSGHMIFLDHTTTGDGTLAAL
                     QLLAVMKRKEKKLSELAKIIELYP"
     gene            complement(1150..2157)
                     /locus_tag="VI935_09725"
     CDS             complement(1150..2157)
                     /locus_tag="VI935_09725"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HLE25912.1"
                     /translation="MGKDGYNINEFLDWLKAELNSWDLEHLKDLLFKNLGKKILAVVF
                     ALALWFSANMEQDVEKTISVDVQYMNLPSDLVILNDPPHTLNLRVRGSRTQLSSLSPR
                     DIVFAFDLANASLGISKFEISTDQIKPPKGIQVIGVSPAEIRIDIDKVIEKNVSVNPI
                     IGLPDTGYEVVGEPKVTPSEVTISGPRKIVSQIESISTDLVSTTGVKSNFTMEVPLMS
                     YPGVDIIGGGFARVTVSMKEKIATKEFKGLNINIINFNGFKFEPRGPLKAELLFEGPY
                     SIIKDLNSDDINVFVDLNKLKPNSKQKIQKLKVNVDYPYKGSLILKKKIPDIIEVRLE
                     K"
     gene            complement(2298..3131)
                     /gene="cdaA"
                     /locus_tag="VI935_09730"
     CDS             complement(2298..3131)
                     /gene="cdaA"
                     /locus_tag="VI935_09730"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HLE25913.1"
                     /translation="MLDAISNFRYFWDVIDIIIVALIIYYLLSLIEGTRAVQMLFGLA
                     AVFVLYFLSQRWELYTLNWILGHFLGSIILIAVILFQQDIRRALAAFGGNPLVLKIYH
                     GKSEELVVDEIVKATSFLSNRQVGALIAIERKNSLADFVEIGMRVDAWVSRELIISIF
                     NPTSPLHDGGVIIGRNKILSVGSFFPLATDPDLERELGTRHRAAIGLTRETDSVVVIV
                     SEETGTISLAFQGNLTRGLDATSLNNRLLDLLEIKRGQTDRQRIFSRILRRKHAGKTS
                     A"
     gene            complement(3167..4039)
                     /gene="folP"
                     /locus_tag="VI935_09735"
     CDS             complement(3167..4039)
                     /gene="folP"
                     /locus_tag="VI935_09735"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HLE25914.1"
                     /translation="MKKATLFKENIHFKCKDKELHLGSRTFIMGILNVTPDSFYDGGR
                     FLNSDDALVQIDKMMNEGADIIDIGGESTRPGSLGVTDEEELRRVVPIIKEAMKRFDV
                     ILSIDTTKPRVAEEALGEGASIINDISGLKFNPEVAQIAAKCGAGLVLMHTSSHPYDM
                     QLKTEYKSFIPDVINSLNRSIEIAESNGVNKKSIIVDPGFGFGKRIEHNFTLLKHLSE
                     FLVFDKPILIGTSRKSFIGRILGASVEDRLEGTAATVAIGIMNGASIVRVHDVLYMKR
                     VAKIVDAVMSASFN"
     gene            complement(4193..>4888)
                     /locus_tag="VI935_09740"
     CDS             complement(4193..>4888)
                     /locus_tag="VI935_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HLE25915.1"
                     /translation="DKVLMGVERKSLIISDAEKKITAYHEGGHALVAKLTPGTDPIHK
                     VTIIPRGLALGVTQQLPIEDKYTLSKTYLNNSITVLLGGRAAEEIIFGDQTSGAGNDL
                     ERVTEIARKMVCEWGRSDKVGPVTFGKREEQIFLGKELARHKDYSEATALEIDKEIKR
                     IVTENYKRAKTILEENIDLLHKLAEVLLEREVVDGKELDELVKQRRPEYTSWEGSLSA
                     KEAVARRSNVEDS"
BASE COUNT         1360 a         1120 c          801 g         1607 t
ORIGIN      
        1 ggatagagtt caattatttt tgcaagctct gagagttttt tttcctttcg tttcattact
       61 gctagtaatt gaagagctgc gagcgtacca tctcccgttg tagtatgatc aagaaatatc
      121 atatgccctg acttctcacc cccgaaatta caacccctta tcctcatctc ctctacgaca
      181 tatctatccc ctacaggtgt tctaataact attccaccct tttctcgtat gaaggtctca
      241 agggacatat tgctcataat cgtcgtgaca agggtttttt tttggagttt gttgtttctt
      301 atcatttcgt cagccgttat tgcaattact atatccccgt caacttcttc acctttttca
      361 tcacagaata taaccctatc ggcatcacca tctaacgcta ttcccagatc cgcaccagta
      421 tctttaacct tgcgtttaag tagttcaggg ttaatagaac cacaatctgt attaatattc
      481 ttgccatcag gtctaacccc gatcgctatc acttctgccc cgagttcttg aaaaacaatt
      541 ggagaaacct tataagccgc tccattagcg cagtcaagaa taattttaag tccttcaagg
      601 gtaagatcct ttgggaatgt attctttaca aatacaacat accttccctg agcgtcgtcg
      661 agtctccaag cctttcctat atctgaaggg gaaacattca gacggttaat tctatcgcct
      721 aaaaccaatt cctctatctc taattctttt tcatcaggta atttgaaacc atttctgtcg
      781 aagaatttaa tcccattatc cacaaatggg ttatgagagg ctgatattac aacgccagca
      841 tcagcacgca tgcttactgt aagaaaagat atagcaggtg tgggtagggg accaactaga
      901 agaacatctg ctcccattga tataattcct gctgacattg cctgttcaaa catgtaccct
      961 gatagccttg tatctttacc tataactatt cttggcattt ttgaatctgt cttttccatt
     1021 ttaagtatat aggttagtgc cttcccaagc tttagactca tctcaggagt cattgggtgg
     1081 caatttgcta ttcctcttat cccgtctgta ccaaaaagat gcttacctga ctcgttcaac
     1141 cttgttctcc tatttttcta atcttacttc aattatatca ggtatctttt tcttaagtat
     1201 aaggcttccc ttataaggat aatctacatt tacttttaat ttctgtatct tttgttttga
     1261 gtttggctta agtttattta gatcgacaaa cacattaata tcatcactgt ttagatcctt
     1321 aataatgctg taaggaccct caaataataa ttctgcttta agtggaccgc gtggttcaaa
     1381 cttaaacccg ttaaaattaa ttatatttat atttaaaccc ttgaactcct tcgttgcaat
     1441 cttctctttc atactaactg taactctagc aaatcctccc ccaataatgt caacccctgg
     1501 ataggacatt aaaggaactt ccattgtaaa attagatttc accccagtcg ttgatacaag
     1561 atcagtagat atactttcta tttgagaaac aatcttcctt ggaccactaa tcgtaacctc
     1621 agaaggtgtt acctttggtt caccaactac ttcataccca gtatccggaa gaccaattat
     1681 agggttaaca gagacgttct tttctattac tttgtcgata tcaattctaa tttctgcagg
     1741 gctgactcca attacctgaa tcccctttgg cggctttatt tgatcagtac taatctcaaa
     1801 ttttgaaatc cctagggaag cattagccag gtcaaatgca aaaacaatat ctcgtggaga
     1861 aagggacgat agctgtgttc tagatccacg aacccttaag ttaagtgtat gaggtgggtc
     1921 attcagaata acaaggtcgg aaggcagatt catgtattgc acatctactg atatggtttt
     1981 ttctacatcc tgttccatgt tcgctgagaa ccagagggct aatgcaaaga caactgcaag
     2041 aatcttttta cctaggtttt taaatagtaa atcctttaaa tgctcaagat cccatgagtt
     2101 taactcagcc ttaagccaat ctaagaactc atttatatta tagccgtctt ttcccatgac
     2161 tgttaaatcg ttatttctgc caatgatacc gaaggcccta aaatgggcta cattggttaa
     2221 agagaaatat atagaagctt aagtaactct actttattaa aatctacaaa cagcccaata
     2281 aaaaggataa atgctgccta ggcgcttgtt tttccagcat gtttcctgcg taaaatacga
     2341 ctgaatattc tttgcctatc tgtttgtcct cttttaatct cgagcagatc aagtaggcga
     2401 ttgttgagtg atgtcgcatc gagtcctcta gtaagattgc cctggaacgc aagggatatt
     2461 gtccctgttt cctctgaaac tataacgaca actgagtccg tctctcttgt aagcccgatc
     2521 gctgctcgat gtcttgtacc cagttccctt tccaaatctg gatcagttgc aagtgggaag
     2581 aaagacccta cagatagtat cttatttcgc cctataatga ccccaccatc gtgaagtggt
     2641 gaagtcggat tgaatatgct tataattagt tctcttgata cccatgcatc aaccctcatg
     2701 ccaatttcaa caaagtctgc aaggctgttt tttctttcaa ttgcgataag agcaccaacc
     2761 tggcgattcg ataaaaagct tgttgccttt acgatttcgt caacaacaag ctcctccgat
     2821 tttccgtggt atattttcag gacaagcggg ttcccaccaa aggctgcgag ggctctccga
     2881 atatcctgtt gaaacagaat tactgcaatt agtattatag agccgaggaa atggcccagt
     2941 atccagttta atgtgtagag ttcccatctc tgtgaaagaa aataaagcac aaaaactgct
     3001 gcaagaccaa aaagcatttg aaccgcacgt gttccctcaa tcaggcttaa aagataataa
     3061 ataatcaggg caacgattat aatatcaatc acgtcccaaa agtatctgaa attagagatt
     3121 gcatctaaca ttgttgaatc ccttcttgaa tcccttataa aaatccctaa ttgaagcttg
     3181 cactcataac tgcatctact atcttagcta ctcgcttcat ataaagcaca tcgtgaaccc
     3241 taacaatcga ggcgccattc ataatgccaa tcgcaacagt agcagcagtt ccctctagtc
     3301 gatcttctac tgaagctcca agaattcttc cgataaagga cttacgcgac gttcctatga
     3361 gaatcggctt atcaaataca agaaactcgc taaggtgctt tagaagagta aaattatgct
     3421 ctattctttt cccaaatcca aaaccagggt cgactataat gcttttcttg ttaaccccat
     3481 tgctttctgc tatttcaata gatctattaa gagaattaat gacatcagga ataaacgatt
     3541 tatactcagt cttaagctgc atatcatagg gatgagagct tgtatgcatt aaaacgagtc
     3601 ctgctccaca ttttgctgct atctgagcaa cctccgggtt aaatttaagc ccacttatat
     3661 cgttgattat tgacgctcct tctcccaaag cctcttcagc tactctgggc ttagttgtgt
     3721 ctattgatag aattacatca aatcgtttca tagcttcttt tataattgga actacacgcc
     3781 tgagttcttc ttcatccgta accccaagtg acccaggtct agttgattct cctccgatgt
     3841 ctataatatc cgccccttcg ttcatcattt tatcaatctg tacaagtgca tcatctgagt
     3901 taagaaacct cccgccatca taaaatgaat ccggggtaac atttaaaatc cccatgataa
     3961 atgttcttga acccaggtgt aattctttat ccttacattt aaaatgtata ttttctttga
     4021 ataaagtagc cttcttcata attcaatgct ttgcatttaa attggctatt agtatttgaa
     4081 ttatatttat tcattacaac cctttttaat ctttatgacg atctatccaa gttgaaactt
     4141 taccaggatg acaagtagtc ttgataagat tttacttaaa cagctttaac ttttaggaat
     4201 cctcaacgtt agatcgtcgg gccactgctt cttttgcgct aagtgaacct tcccacgaag
     4261 tgtattcagg ccgcctttgc tttacaagct catctagctc tttaccatct accacttctc
     4321 tttcgagaag cacctctgca agcttatgaa ggaggtcaat attctcttct aggattgttt
     4381 ttgccctttt gtaattttcg gtaactattc tctttatttc cttgtcaatt tccagcgccg
     4441 tagcttcact atagtcttta tgccgcgcaa gctctttgcc aagaaagatt tgctcttctc
     4501 tttttccaaa tgttactggt ccaaccttat cactcctccc ccattcacac accatcttac
     4561 gagcgatctc tgttaccctc tcaaggtcgt ttcccgcacc acttgtctgg tctccgaata
     4621 ttatttcttc cgcagccctg ccaccaagaa ggacagtaat tgaattgtta agataagttt
     4681 ttgaaagtgt atatttatct tctataggaa gctgttgtgt tactccaagt gcaagtcctc
     4741 ttgggatgat agttacctta tggattggat ctgttcctgg ggtgagctta gcaacaagcg
     4801 cgtgcccacc ttcatggtac gctgttatct tcttctcagc atcactaatt ataaggcttt
     4861 ttctctcaac tcccatcagc actttatc
//
LOCUS       DATDRL010000290         3192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1684669, whole genome shotgun sequence.
ACCESSION   DATDRL010000290 DATDRL010000000
VERSION     DATDRL010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684669"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VI935_09745"
     CDS             complement(<1..660)
                     /locus_tag="VI935_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HLE25916.1"
                     /translation="MVPLQLSLKNFLSYSEDVPILDFRGFTIACLSGKNGHGKSALLD
                     ALTWALWGKCRAKNKEEVIKRGAKEARVELEFELEGNRYRVIRTIIRTKGSATTASLD
                     FQVFDTSTESFRPLSQGGQAQYAIEKTLKMDYDSFICSAFILQGRADEFTKRTPAERK
                     DVLARTLGLERYEDLAKKARELAQKSKIEGEQVERDITNVESEISQKDNLIRKLNETK
                     AE"
     gene            774..1754
                     /locus_tag="VI935_09750"
     CDS             774..1754
                     /locus_tag="VI935_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HLE25917.1"
                     /translation="MELSEILEIIAPNLEEVEGVIEENIQSEVPLVYEISRYILGSGG
                     KRLRPSVLILSSGACGFNKGKEIIASAAALELIHTATLLHDDVVDDANIRRGKTASNV
                     IWGNKTSILVGDFMLARALGLIQSCGNLELIKAVTDAAAKLAEGQVLEVMSAQSMIEV
                     SEKVCFDIIEHKTASLIESCGMVGAIIAGAREDVKEAIGNYGLNLGIAFQLVDDALDY
                     SSQEEEIGKGVCQDLKEGKMTLPLVYALRRASTREKNRLIEILQRDGFKNEELDFVLN
                     VVDKYRGVEQTKTEAKKFADKARSSISNLPANNHKSSLQHLADYVVERRT"
     gene            1888..>3192
                     /locus_tag="VI935_09755"
     CDS             1888..>3192
                     /locus_tag="VI935_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HLE25918.1"
                     /translation="MARKKGEWEKRVKAKERQAEFRTPSGIVLESLYKPGDIENLDYE
                     SDLGYPGEYPFTRGVQPTMYRGRFWTMRQYAGFGTAEETNERFKYLLEQGQTGLSVAF
                     DLPTQMGYDSDHPISRGEVGRVGVAIDSLEDMEILFEGIPLGEVSTSMTINATASMLL
                     AMYTVAAEKQGVGPDKIQGTVQNDLLKEFIARGTYIFPPRDSVKITIDVMEYCRDSIP
                     KWNPISVSGYHMREAGSTAVQEVAFTLADAIEYLGHAKERGLDIEKIAERVSFFFAVH
                     NNFLEEVAKFRAARRLWARTMKERFEVKDDRACMLRFHAQTSGVTLTAQQPRNNIIRV
                     TIQALAAALGGTQSLHTNSFDEALALPSEEAATIALRTQQVIAYENGAGDTIDPLAGS
                     YCIEALTNKIEEESIKYIEQIDKRGGMIRCIEEGYINREIEDA"
BASE COUNT          950 a          584 c          753 g          905 t
ORIGIN      
        1 ttctgctttg gtttcattga gttttctaat caaattatct ttttgcgaga tttcggattc
       61 gacattagtt atatctcttt ctacttgttc tccttcgatt tttgattttt gagcgagttc
      121 tcttgccttc tttgctaaat cttcatacct ttctagtcct aaggtccttg caagaacatc
      181 ttttctttca gcaggcgttc tttttgtaaa ttcatctgcc ctcccttgaa ggataaaagc
      241 tgaacagata aaagagtcgt aatccatttt aagggttttc tcaattgcgt actgggcctg
      301 tccaccttgg cttaggggtc tgaaggattc tgtgctggta tcaaaaactt gaaagtctaa
      361 agatgctgtg gtcgcactcc ccttagttct aattatagtt cttataaccc tgtagcgatt
      421 tccttcaagc tcgaattcaa gctcaaccct agcctcttta gctcctcttt ttatcacctc
      481 ttctttattc ttagctctgc atttgcccca tagtgcccaa gttaatgcat caagaagggc
      541 tgatttgccg tgaccgtttt taccagatag acaggctata gtgaatcctc taaagtcaag
      601 tataggtacg tcttcactat agctcagaaa gttctttaaa gagagttgta gaggaaccat
      661 tccgtggagt aactagatat taaaatacat tacgcattat taaaataaaa gtccgagagg
      721 acctagtctg catgttttga cttaaatggc ttgttcagat aatttaaaaa ggcatggaac
      781 tttctgaaat tctagagatt attgcaccaa atttagaaga agttgaaggg gtaattgaag
      841 aaaatattca atcagaggtt ccacttgttt acgaaatttc aagatacata ttgggaagcg
      901 gtgggaaaag actacgtcct tcggtgctga ttctttcgag tggcgcatgt ggatttaaca
      961 aaggaaagga aataattgcc tcagccgcag ctcttgaact tattcataca gccacgctcc
     1021 ttcacgatga tgttgttgac gatgcgaata taaggcgcgg caaaacagcc tcaaatgtaa
     1081 tttgggggaa caaaacaagc atacttgtag gagattttat gttagctcgt gcccttgggt
     1141 taatacaatc ctgcgggaac ctagagctta taaaggctgt aactgatgct gcggcaaagc
     1201 tggctgaagg ccaggtgctt gaagtcatga gtgcacaaag tatgattgaa gtatctgaga
     1261 aggtctgctt tgatattatt gaacataaga cagcatctct tatagagagc tgtggaatgg
     1321 taggggccat tattgctgga gctagggaag atgtgaaaga agcaattggg aactatggtc
     1381 ttaatttagg tattgcattt cagctggtag acgatgcact tgactattca tcgcaggagg
     1441 aagaaatcgg aaaaggtgtg tgtcaggatt taaaagaggg aaagatgact ttgccgctag
     1501 tttatgccct tcgacgtgct tccaccagag aaaaaaaccg tcttatagag attcttcaaa
     1561 gagacggttt taaaaatgaa gaactcgact ttgttctaaa tgtcgtagat aagtatagag
     1621 gtgttgaaca aacaaaaact gaagcaaaga aatttgctga taaggcgagg agttcgatat
     1681 ccaatcttcc ggctaacaat cataaaagtt ctcttcaaca tcttgcagat tatgtagtgg
     1741 aaaggaggac gtagaccttc gtgattctgg tctcgtggtc cgtttttcat agttcgagtc
     1801 aagaaaccca agccacgaat cacgcgaatc caatgacagt tattgcaggt aatatggaat
     1861 tatatccttt gagaaggaga aaaagggatg gcaagaaaaa agggtgagtg ggagaagcgg
     1921 gtaaaagcaa aagagagaca agctgagttt cgtactcctt cggggattgt tctagaaagc
     1981 ctatataaac caggggatat agaaaatctc gactatgaaa gtgatctagg ctaccctggg
     2041 gaatatccct ttactcgcgg agtccagcct acgatgtatc ggggaaggtt ttggacaatg
     2101 cgtcagtatg cgggatttgg cactgctgaa gaaactaacg aaaggtttaa atatcttctt
     2161 gagcaaggac agactgggct cagcgttgct tttgaccttc caactcaaat gggatatgac
     2221 tcggatcatc ctataagtag aggtgaggtt ggtagggttg gtgttgcaat cgattcgctc
     2281 gaggatatgg aaattctttt tgagggaata cctctcggag aagtcagcac ctcgatgaca
     2341 attaacgcta cagcctcaat gcttcttgcg atgtatacgg tggctgcaga aaagcagggt
     2401 gtcggtcctg ataaaataca gggtaccgtt caaaatgatc tcctaaagga attcatagca
     2461 aggggaacat atatattccc tccaagggat tctgtaaaaa ttacaataga tgttatggaa
     2521 tattgtcgtg atagcatccc gaagtggaat ccaataagcg taagcggcta tcacatgaga
     2581 gaagcaggtt ctacagccgt tcaagaagtt gcatttaccc ttgctgatgc tattgagtac
     2641 cttggacatg caaaggaaag ggggcttgat atagagaaga ttgctgagag ggtatcattt
     2701 ttctttgctg tgcataataa ctttcttgag gaggtggcaa aattccgtgc agctcgaagg
     2761 ctatgggcaa gaaccatgaa agaaagattt gaggtaaagg acgacagggc atgtatgctt
     2821 aggtttcatg ctcaaacatc gggtgtgacg cttacagctc agcagccaag gaataacatt
     2881 attagagtaa ctatacaagc acttgctgcc gcactgggcg gaacgcaatc gcttcatact
     2941 aattcatttg acgaggctct tgcacttccg tcagaagaag ccgcaacaat tgcacttcgt
     3001 acacagcagg ttattgctta tgagaacggc gctggtgaca caatagatcc ccttgcaggt
     3061 tcttactgta tcgaggctct tactaataaa atagaggagg aatccattaa atatatcgag
     3121 cagattgata aaagaggtgg gatgataaga tgcatcgaag agggatacat caaccgtgaa
     3181 atagaagatg ct
//
LOCUS       DATDRL010000291        19552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2208811, whole genome shotgun sequence.
ACCESSION   DATDRL010000291 DATDRL010000000
VERSION     DATDRL010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 19552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19552
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2208811"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VI935_09760"
     CDS             complement(<1..273)
                     /locus_tag="VI935_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HLE25919.1"
                     /translation="MGSEINNLNQNRKFRTALLSIIVSLFLIITKLIVGFLTNSISIF
                     ASAVDSFVDVAASSINYFSIIKAEKPADKEHRFGHGKAEGLAGLFQS"
     gene            complement(474..1727)
                     /locus_tag="VI935_09765"
                     /pseudo
     CDS             complement(474..1727)
                     /locus_tag="VI935_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
     gene            complement(2179..4158)
                     /locus_tag="VI935_09770"
     CDS             complement(2179..4158)
                     /locus_tag="VI935_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016864374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HLE25920.1"
                     /translation="MFIEFGREICGELSTAETREWLVTNGIGGFASGTVAGLLTRRYH
                     GLLIAALKPPLGRTLLVTKLDETVQYNGRRFPLNTNRWGDGTVDPHGYRYIENFHLEG
                     TIPVWRFACGDALLEKRIWMQLGTNTTYVHYNLYRATRPITLEIKAIVNYRDYHSTTH
                     GGNRQMKIDKVEHGISVTAYTGAQPFFLFTDKADALPTHDWYLGFDLVLERYRGLDNR
                     EDHLHAATFSVTLNPGESLTFVASTELNPSLNGEEALELRRSYEKKLVNLLNERHLLS
                     SGVSPEWISQLVLAADQFIVIRPLPNEPGGKTIIAGYHWFGDWGRDTMISLPGLTLST
                     SRPEIAQSILRTFAKYVDRGMLPNRFPDAGENPEYNTVDATLWYFEAIRAYYDATGDN
                     DLLHELFSVLAEIITWHTRGTRYNIHVDSKDGLLYAGEKGVQLTWMDAKVGDWVVTPR
                     IGKTVEVNALWYNALRTMTKLARQLKKPYKDYEAAAKLAFKGFSRFWNDEAGYCYDVI
                     DGPDGNDASLRPNQILAVSLLESPLNPDQQRGVVEACARHLLTSHGLRSLTPEHPQYQ
                     GHYGGDQRQRDGAYHQGTVWGWLLGSFALAHLRVYNDPVKAREFLEPISRQLLSHGVG
                     SLSEIFDGDAPMKPRGCIAQAWTVAEVLRAWLATV"
     gene            complement(4250..6991)
                     /locus_tag="VI935_09775"
     CDS             complement(4250..6991)
                     /locus_tag="VI935_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016869519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HLE25921.1"
                     /translation="MTHEEKRLEEESNRKAYWRRWGPYLSERQWGTVREDYSPYGTAW
                     DYFTHEQARSRAYRWGEDGIAGISDNHQRLCFAIALWNGNDPIIKERLFGLTGSEGNH
                     GEDVKEYYFYLDNTPSHSYMKYLYKYPQRAYPYSQLVAENRRRGRMEPEYELLDTGVF
                     EGARYFDVFVEYAKNLPDDIVIQIRIINRGPEEATLHLLPTLLFRNTWSWNHSDEKPS
                     LKVIKITSHTSVIEASHPTLGERWLYCEAPIELLFTENETNSEKLFGVNNPSPYVKDG
                     INDYIVDGRKEAVNPNQIGTKMSAHYSLKIGVGETQTVRLRLSQFKSLVEPFSPCFEA
                     ILQDRMREADEFYERICPFPLTDDMRNVQRQAFAGMLWNKQFYNYVVEDWLKGDPAEP
                     PPPPERKHGRNHSWIHLYNDDILSMPDKWEYPWFAAWDLAFHTIPLVMVDPDFAKRQL
                     ILLTREWYMHPNGQIPAYEWAFSDVNPPVHAWAAWRIYNIERKIYGQEDRLFLERVFQ
                     KLLLNFTWWVNRKDSEGRNVFEGGFLGLDNIGVFDRSAQLPTGGHIEQADGTSWMGMF
                     CLNMLAIALELAKENPSYEDIASKFFEHFIYIANAINIIGGEDAGLWNEEDGFYYDVL
                     HLPDGRHLPMKVRSMVGLIPLFAIETLEPKTLDKLRGFKNRLEWFIDNRPDLKQNVAC
                     MRTQGMAERRLLSIVNKDKLRRILQKMLDENEFLGPHGIRAVSRFHSGNPYIFHINGH
                     EYRVDYEPAESSSGLFGGNSNWRGPVWFPVNFLLIEAIQKFHHYLGDDYKVECPTGSG
                     NMMTLWEVATELSNRLMKIFLKDSSGRRPVYGGAEKFQTDPHWRDYILFYEYFHGDNG
                     AGIGANHQTGWTGAVAKLIQQYGEYHVRQKPPEIIDQVLDRGKTRDRKKGIEYKKTG"
     gene            complement(7112..8218)
                     /locus_tag="VI935_09780"
     CDS             complement(7112..8218)
                     /locus_tag="VI935_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLE25922.1"
                     /translation="MKAIAVIPGKPNSVHLRDVPKPKVSDVPNGRGVFVKILRVGVDG
                     TDKEINAAEYGASPPGDDYLILGHESFGIVEEIGPNVTELQSGEYVVATVRRPGSSPY
                     DQIGMYDMTTDDVYYERGINLRHGYLTEYYVDAPEYIINVPKGLKEVGVLLEPTSVIE
                     KGIYQAYEIQRRLRIWHPKRAAVLGAGTIGLLATMALRLRGLEVCTFGLTKPPYLNSD
                     LIGEIGGCYHSTKDITLTESAKEHGPYNLIFEATGYSPIVFEAMNIVGKNGVLVLSSV
                     TGGGRSVEIPADMINLGFVLGNKVVVGTVNANRDYFEIGVKDFSQAELQWPGWLPKFL
                     THPVKGLENYQDLINKLTEAKGAIKVFCEVAPIS"
     gene            complement(8223..8387)
                     /locus_tag="VI935_09785"
     CDS             complement(8223..8387)
                     /locus_tag="VI935_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25923.1"
                     /translation="MVLRFVYEPKWIIRNYHPVYTSVNRRMDGQYRFFETAIKDNASN
                     VTVSERSGGG"
     gene            complement(8392..8583)
                     /locus_tag="VI935_09790"
     CDS             complement(8392..8583)
                     /locus_tag="VI935_09790"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HLE25924.1"
                     /translation="MAKEKVSSQKKPRRKMELDERTLQEMIAKKAHELYEKRGGGHGS
                     DLDDWLEAERIVMGKKRKK"
     gene            complement(8658..8858)
                     /locus_tag="VI935_09795"
     CDS             complement(8658..8858)
                     /locus_tag="VI935_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25925.1"
                     /translation="MAKDVKSKHKKMICPSCGVEMNYHADKIDYTAALTHPDKMDPDL
                     GGILEEVHTCPKCGGTEVRMGT"
     gene            complement(8935..9501)
                     /locus_tag="VI935_09800"
     CDS             complement(8935..9501)
                     /locus_tag="VI935_09800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HLE25926.1"
                     /translation="MNSNRLIFIPVILGTSRQGRMSEHVAKFILEEVSKRDGVETELI
                     DIRNLSLTTSDAGENIKNKKFSETVMRADALIIIAPEYNHGYPGLLKHVLDTNLKEYI
                     HKAVGICGVSAGGFGGTRVIQNLLPVMRELGLVTIFWDGNFSSVQNIFDSSGKLLDHS
                     YIKRIDKFLKELIWMARVLRYGRDNVPL"
     gene            complement(9525..10562)
                     /locus_tag="VI935_09805"
     CDS             complement(9525..10562)
                     /locus_tag="VI935_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLE25927.1"
                     /translation="MRIKQTFLPLWLLLVIGLLAQTALSQQPSQQPKVMAVDAIGITV
                     SDMDRTVDFYSNVLSFKKVLDIVVEGEGYEELLGLPDLRIRFVRMQIGDEFISLIQYI
                     EPSDGKPIPVDSRSNDLWFQHFAIVVSDMDKAYQHVNKHKVSQISTSPQTLPKSNKPA
                     AGIRAFKFKDPDSHPLELLWFPPGKGDPRWHRPTDKLFLGIDHTAIGVNDTEASLRFY
                     RDLLGMNVLGGSLNIGTEQDNLDNVSGAKVRITSLRPSANIPGIEFLEYVSPENGRPI
                     PSEIKANDIVHWQTILIVDDVEMAEEWLRNRKVRFVSDQVSTLPNGKLGYKNGIKVFD
                     PDGHAILIVEK"
     gene            complement(10591..12171)
                     /locus_tag="VI935_09810"
     CDS             complement(10591..12171)
                     /locus_tag="VI935_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015206746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLE25928.1"
                     /translation="MPQDTHYDVIIIGSGAGGGTLAYHLAPSGKRILLLERGDYVPRE
                     KDNWEPRAVNVEGKYQTKEVWRDKEGKPLHPHTNYYVGGNTKFYGAALFRLRKEDFGE
                     IRHHGGVSPAWPISYDELEPYYTKAEHLYQVHGQRGVDPTEPPTGAPYLYPPVSHEPR
                     IQQLSDDFAHLGLRPFHTPLGVMLDEKNMHNSRCIRCNTCDGFPCLVYAKSDAHVLCV
                     DPALKYPNVTLLTHAFVSRIETSASGKEVTEVTKVYVERNGAKETYSANIVVISCGAI
                     NSAALLLRSANDKHPHGLANSSDVVGRHYMGHVNSVLMAISRCPNPTIFQKSLSVNDF
                     YFASKEWEYPMGHISFVGKLDAETLKAGAPALTPGWTLEYIAKHSLDFWLTSEDLPDP
                     ENRVTLDSEGNIVLAYKQNNEEGHKRLIKKLESLMKQQTKCPEHGHDCHQGLFSRNLF
                     VGQRIPLAGVAHQNGTIRFGRDPKTSALDVNCKAHDLDNLYVVDASFFPSSGAVNPAL
                     TIIANALRVGDHLLERLR"
     gene            complement(12177..12683)
                     /locus_tag="VI935_09815"
     CDS             complement(12177..12683)
                     /locus_tag="VI935_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HLE25929.1"
                     /translation="MKRTIAIIVILIIIVVLWYLFRPEKLYISETVQEEFPITAQETT
                     GSKSSPVVLVTGNFHGVAHDGKGTATIYQLPDGKRVLRFTEFETSNGPDVQVYLVAAQ
                     DANDNDTVKQAGFVSLGSLKGNIGDQNYDVPADVDLSKYKAVTIWCQRFGVNFGTAPL
                     TPQQHQGS"
     gene            complement(13165..14220)
                     /locus_tag="VI935_09820"
     CDS             complement(13165..14220)
                     /locus_tag="VI935_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HLE25930.1"
                     /translation="MKVKRKILSSGFLNFIQAMVIIVIILQIVLARAEEVNSSTATSG
                     TIVRLDPRLNQLFPQGAVLEKLAEGFTWVEGPVWNREGGYLLFSEIPSNSVLKWKEGE
                     GVSLYLKPSGYTGSALFKGKEPGSNGLLFDSSGMLVLCEHGDRRVSRLEKNGTKTTLA
                     DRYEGKRLNSPNDAVYKSNGDLYFTDPPFGLPETFNDPAKELPFQGVYRLSSDDKLTL
                     LTKDIKAPNGIAFSPDEKILYVTDVDPKRSAWLAFDVKDDGTITNGRVFYDAMPFTKR
                     MKGAPDGFKVDINGNLFAAGPGGVYVFSPDGTHLGTIVTGVPTSNVGWGNDGSVLYIT
                     ASDSIYRIKLNTKGKGF"
     gene            complement(14387..16027)
                     /locus_tag="VI935_09825"
     CDS             complement(14387..16027)
                     /locus_tag="VI935_09825"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HLE25931.1"
                     /translation="MTSRSIHLILFSILVSFLCVIGVLAQTNQDVPSGIPNASIDLST
                     KEGVNLVKGQWRYSDVEIIQVDFKAPGPDGQPTGKPVKTYDYKPHAGGVDFDDSKWEV
                     LDPTSLNKRRSTGRICFNWYRINIAIPKRIGNFDPTRSTVVFETSLDDYAEIWVDGEI
                     PRALGQSGGSVIAGWNASNRIVVGRDVRPEQKIQLAIFGINGPISNPPTNFIWMRYAR
                     LDFYKGSKVPIAVTPQEVNVEVARLDSAMDAIVGPNPKIFKLAEGFKFTEGPIWVSNE
                     GYLLFSDPNSNIIYKYTKDGMLSVFRQPSGYSGADVAEYGQPGSNGLTLDAQGRLTID
                     EHGNHRVSRLEKDGALTVLVDSYEGKRLNSPNDLVYKSDGTLYFTDPPFGLPKFFDDP
                     RKELPFSGVYSFKDGKLTLLIKDLKGPNGIAFSPDEKFLYMGNWDPETKVVMRYEVNA
                     DGALSNGKIFFDMTSAPGEDAIDGVKVDQQGNIYVSGPGGLWVISPEGKHIGTIVAPK
                     HIHNMAWGGEDGKTLYLCARSGLYRMQLNIPGVRPSTN"
     gene            complement(16024..16512)
                     /locus_tag="VI935_09830"
     CDS             complement(16024..16512)
                     /locus_tag="VI935_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HLE25932.1"
                     /translation="MKFIISLVLLLLLLAPSILAELPSKHVLTLKVAKKIAEAAEAEA
                     IKRGAKVVIVVVDDGGYLVLLERLDNTQVASVEVGIGKARTAAIFRRPSKVFEDQVRE
                     GRVAALGLPGATPLQSGIPIIYDGKVIGAIGVSGETPQEDEDIAKVGASAIDAVIKGG
                     DK"
     gene            complement(16781..17647)
                     /locus_tag="VI935_09835"
     CDS             complement(16781..17647)
                     /locus_tag="VI935_09835"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HLE25933.1"
                     /translation="MNLKNFVIVFVLLIMSTASHAQVANKKSLTLEGAMKVIATAVDY
                     AKKNNAPGAAIAVVDEGGNLIALERLDGTFAAGANISIGKARTAALFKKPTKVFEDII
                     AKGRTSMVALQDFTPLQGGVPITLGGEIVGAIGVSGAASAQQDEEIAVAGANAIGSSS
                     ASMMTAPSAQVTYFDNSKVSAAFAKGMPLLEIANYKIHASRRTEPGMAEVHVKDTDII
                     YMLEGSATLVTGGTVVDGKTTAQDEIRGKEISGGETRTITKGDVIVVPAGTPHWFKAV
                     QAPSLYYVVKVS"
     gene            complement(17781..18236)
                     /locus_tag="VI935_09840"
     CDS             complement(17781..18236)
                     /locus_tag="VI935_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25934.1"
                     /translation="MRVFDLNKVLIGGITMDNLGTFRRIRMGLFSSLIAVLFLATISS
                     FAADMHTSTVFKGVKVNGGTVTHTKQDGKNVLTLSDDFKVPGTPDPHWQVIDSKGNVY
                     LLQRLDIKGGKVNKSITLPSYIPDVVKVQIWCAFAETLLGEASFASPVM"
     gene            complement(18262..19095)
                     /locus_tag="VI935_09845"
     CDS             complement(18262..19095)
                     /locus_tag="VI935_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011242341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HLE25935.1"
                     /translation="MAVTQTNTEKLTMIKALSQHWPEYFIEASGLGLFMISACFFGFV
                     LEHPDSPLHQALPDPFLRRVLMGMAMGLTAVGIIYSPWGKQSGAHINPSVTLTFFRLG
                     KVKFWDAVFYIIAQFVGAVIGVLIASTLMGQLISHPSVNYVATLPGMVGNGIAFLAEL
                     IISFILMFVILIVSNTNDIARYTGLFAGVLVAVYISLEAPISGMSMNPARSFGSAFHA
                     QLWNFLWIYFTAPPIGMLLAAEAYLRLRGPKNIFCAKLHHQNDKRCIHCGYPGAVKTA
                     S"
BASE COUNT         5451 a         4700 c         3684 g         5717 t
ORIGIN      
        1 actctgaaat aaacctgcaa gtccttcagc cttaccatga ccgaatctat gttccttatc
       61 ggcaggcttc tcagctttta ttatggaaaa atagtttatt gatgaagcag ctacatcaac
      121 aaatgaatca accgctgagg cgaatatgct aatagaattt gtaagaaaac cgacgataag
      181 ctttgtgatt attaaaaata gtgatacgat tatagaaaga agtgcagttc ggaatttcct
      241 attttgatta agattattta tttccgaccc caaatctata actctaatct gccaggcctt
      301 gaagaaattt gcttaatata gattaagtcg cctatcgtat ttattaatag atttatcgta
      361 tctacctgga aagttctttt gaatgtcatt cctagccaat ggcttcagtt aacaaattaa
      421 cgattagcca cttttctttt ggtatgtctt tttgctttag aagatttttc ggcaaccctt
      481 ctctcaagct tctctctgta ttcttcaatg tctatttcaa ctttagcaga acctgtctgc
      541 attgcggcct gtgctacagc aacagattcc caaacaaaca cccttgggtc aaagggttta
      601 ggaactatat actccggtcc aaactcaaaa tgtgattctc cataggcttt agctaccgat
      661 tcatctgcac cctgtttggc aagactagat aaagcatgag aagctgctac tttcatttct
      721 tcgttaattg atctagctcg tacatccagt gcccctctaa atatgaatgg aaaaccgaga
      781 acattgttta cctggtttgg ataatctgac cttcctgttg ccataatcag atcactcctt
      841 actgattttg catcaggata ggaaatctca gggtcagggt tcgccatagc aaatataata
      901 ggtttgtcag ccatcgattt aaccatttct tttgttacca taccggctac tgaaagccca
      961 acaaaaacgt ctgttccctt cataacctca gcaagtgttc tcatttttgt atccacagca
     1021 agcctctctt tttgaggggt cattccttgt tctcttcctt tataaataac acccctactg
     1081 tcacacataa tcatattttc tctcgtcgca ccaagaagga taaaaaattc agcacaagaa
     1141 attgcagcag caccggcgcc actaaaaacc atcttaactt tgcttagatt cttacccaca
     1201 agctcacagg cattcagaag tcccgcaccc gagattatag cagtaccatg ctggtcatca
     1261 tggaatacag gtatatcaag ttgtttcttt aattcctgtt cgatatagaa acattcaggt
     1321 gcttttatat cctcaaggtt tatccctcca aaagtaggct caagaagttt tacagtctta
     1381 attacctcat caggatcatg tgtatcaagt tcgatatcaa atgcatctat gtcggcaaac
     1441 cttttgaaaa gaacacactt tccttccata accggtttgc ccgctaagga tccaatatca
     1501 ccgagtccta gaacagccgt tccatttgtt acaactgcaa ctaagttacc tttatttgta
     1561 tattcatatg ctaagtctgg gttcctttca atctctctgc aaggttcggc tacccctggg
     1621 gtatatgcca atgaaagatc ccattgagtc ttacatggtt tagtaggcac tacctcaagc
     1681 tttcctttac gacccttact gtgatactct agggcagctt gtttcatatt gatagtcata
     1741 ttggcctccc tataatacta ttctttgaaa tttattaggc aatgaaaatc atataattta
     1801 tgacttgatt tattttataa tatttaaagt aacctgtcca catcgttaat tctagtgacc
     1861 aaaacttaac cgcccttttc ccattttcgg gaatacaacc gcactgtcat tcccaaatgc
     1921 agttattggg aatccattag aaacacaact ggatacctga tacaagcatt cgggtatgac
     1981 attctgagaa tgtagctcac gttccccgaa cgggcattat atgggttgat gagggcatca
     2041 gccctacagt tacatcgcag cttgaagcca ctggtacaaa aaccaaacct tcgtaggagc
     2101 gccatccctg gcgcgataat gggatcgcgg ggttgcaaaa atccgcttat cgggattatt
     2161 tagatcaaaa atatatgttt aaacagtagc tagccatgcg cgaagaacct cggcaactgt
     2221 ccaggcttgg gctatgcaac ctcggggctt catcggcgca tctccatcaa atatttcgct
     2281 aaggcttcca actccatgtg aaagtagttg gcgtgatata ggctctagga attcacgagc
     2341 ttttacagga tcattgtaga cccgcaggtg cgccaaggcg aatgagccta agagccaccc
     2401 ccaaaccgta ccttggtgat atgcaccgtc tcgttgacgc tgatcccctc cataatgtcc
     2461 ttgatattgt ggatgttcag gagtgagact ccgaagcccg tgtgaagtaa gaaggtgccg
     2521 tgcacaggct tctaccaccc ctcgctgttg atcaggatta agcgggctct caagcagaga
     2581 aacagctaat atctggtttg gccgcaacga cgcatcgttt ccatctggcc cgtctataac
     2641 atcgtagcaa tagccggctt catcgttcca aaagcggcta aatcccttaa acgctaactt
     2701 tgcggctgcc tcgtaatctt tgtatggttt tttaagctgc cgtgctaact tagtcatcgt
     2761 ccgtaaggca ttgtaccaaa gggcattcac ctcaactgtt ttgccaattc ggggagttac
     2821 tacccaatct ccgacttttg catccatcca ggttagttgt accccctttt caccagcata
     2881 aagtaatcca tctttggaat caacgtgaat attatagcgc gttcctctag tgtgccaggt
     2941 tataatctct gctaaaacag aaaagagttc atgcaaaaga tcattgtccc ctgtcgcatc
     3001 ataataagca cgaatcgcct caaagtacca tagggtcgca tccactgtgt tgtattcggg
     3061 gttttcgccc gcatctggaa aacggttggg taacatccct ctgtccacgt atttagcaaa
     3121 tgtacgtaga atagattggg ctatttctgg acgactagta gataaggtta gacccggaag
     3181 gctaatcatt gtatctcgtc cccagtcccc aaaccagtga taacctgcaa ttatagtttt
     3241 acctccaggt tcattaggta atggacggat tacaataaac tgatctgcgg cgagtacgag
     3301 ttgactaatc cactcgggag acacacccga ggataaaaga tgcctttcat taagcaggtt
     3361 tactaatttt ttctcataac tacgacgcaa ttcaagagct tcctcgccat ttaaacttgg
     3421 atttaattct gtactagcta caaaagtaag cgattcaccg gggttaaggg taacgctaaa
     3481 ggtagcggca tggaggtgat cctcgcgatt atctaaaccc ctatatcgtt ctagaaccaa
     3541 atcaaatcca agataccaat catgtgtcgg caatgcatcg gctttatcag taaagagaaa
     3601 aaatggctga gccccggtat atgccgtcac cgagattcca tgttcaactt tatcaatctt
     3661 catctgcctg tttccaccgt gagtagtgct gtgataatca cgataattga caatagcttt
     3721 gatttctagg gtaatcggtc tggtcgcgcg gtataaattg tagtgaacat aagttgtgtt
     3781 agttcctagt tgcatccaga tgcgcttctc aagcagtgca tctccacatg cgaatctcca
     3841 aacgggtata gttccttcaa ggtggaaatt ttctatgtac ctataaccat ggggatccac
     3901 tgtgccatca ccccagcggt ttgtattgag aggaaacctc cttccattat attgaacagt
     3961 ttcatcgagt tttgtaacaa gaagcgtccg cccgagtggc ggcttaagtg ctgcaattag
     4021 taatccatgg tatcggcggg ttagtaaacc tgcaactgtc cctgatgcaa aacccccgat
     4081 gccgtttgtt actaaccatt ccctagtttc tgcagttgaa agttcaccgc atatttcacg
     4141 tccaaattca ataaacatta tgtatatatg ctccctttct aaatattaga tgactgagga
     4201 gttgcaatag agaggtgatt agagataatt aacaaagtaa taggaaaatt catcctgtct
     4261 tcttatattc gatacccttt ttcctatcac gtgttttccc cctatccaat acttgatcga
     4321 ttatctcagg cggcttttgt cgcacatgat attcaccgta ctgctgaatg agttttgcca
     4381 ctgcaccagt ccagccagtt tgatgatttg cacctatgcc agcaccatta tcaccatgaa
     4441 aatattcata aaagagaatg taatctctcc agtgaggatc ggtctgaaat ttctctgcgc
     4501 ctccataaac tggacgacga cccgatgaat cctttaggaa aatcttcatg agtctattag
     4561 ataactcagt tgctacttcc cagagcgtca tcatattacc agaacctgtg gggcattcta
     4621 ccttataatc gtccccgagg tagtgatgaa acttctgtat tgcttctatt aggagaaagt
     4681 ttaccggaaa ccaaactggc cctcgccaat ttgaatttcc gccaaatagc ccgctcgagg
     4741 attcagcagg ttcataatcc accctatatt catgcccatt tatgtggaaa atatagggat
     4801 ttcctgaatg aaatctagaa acagcgcgta ttccatgcgg tcctaaaaat tcattttcgt
     4861 caagcatctt ttgaagaatt cttcttagtt tatctttatt cacaatggat agaagccttc
     4921 tttcagccat accttgtgtc ctcatgcagg caacattttg tttgagatcc ggacgattat
     4981 caataaacca ctcaagacga tttttaaaac ccctgagttt atcaagggtt tttggttcaa
     5041 gggtctcaat tgcaaaaagt ggaataagcc caaccatcga acgcaccttc ataggcaagt
     5101 gacgcccatc tggcagatga agaacatcat agtaaaaacc atcttcttca ttccagagcc
     5161 ctgcatcctc acccccaata atgtttattg cgtttgctat ataaataaaa tgctcgaaaa
     5221 acttgcttgc tatatcctca taactcggat tctcttttgc aagctcaagg gctatagcta
     5281 acatattaag gcaaaacatc cccatccagc ttgttccatc cgcctgctct atatgtccac
     5341 ctgtgggaag ctgagcgctt ctatcaaaaa cgccgatatt atctaaccca agaaaccctc
     5401 cctcaaatac attcctccct tcagaatcct tccgatttac ccaccatgtg aaattgagaa
     5461 gcaatttctg aaatactcgt tcaagaaaaa gcctatcttc ctggccatat atctttcttt
     5521 ctatattata gattcgccat gctgcccatg catgaaccgg cgggttcaca tcgctaaaag
     5581 cccattcata cgcaggaatc tgtccgtttg gatgcatata ccactcccgt gtaaggagta
     5641 ttagctgccg cttagcaaaa tcagggtcaa ccataacaag tggtatggta tgaaacgcca
     5701 aatcccaggc tgcaaaccat ggatattccc atttgtccgg cattgagaga atatcatcgt
     5761 tgtagaggtg gatccaactg tgatttcttc catgtttgcg ttctggtggt ggaggcggtt
     5821 cagccggatc accctttaac caatcttcca ccacataatt atagaattgt ttattccaaa
     5881 gcatcccagc gaatgcctgt ctttgtacat tgcgcatgtc gtctgtaaga ggaaatggac
     5941 aaatacgttc ataaaactca tctgcctcgc gcatacgatc ttgtaaaatg gcttcaaaac
     6001 aggggctgaa gggttccact aaacttttga attgacttaa tcgtagccta acagtttgag
     6061 tctctcctac accgattttc aatgaataat gagctgacat ctttgttcca atctgattcg
     6121 gattcaccgc ttccttccgt ccgtccacga tataatcgtt aatcccgtct ttgacgtatg
     6181 gagacgggtt attcacacca aatagttttt cactattagt ttcgttttct gtaaatagaa
     6241 gctctattgg tgcttcacaa taaagccatc tttctccgag cgttgggtgt gaggcttcaa
     6301 tcacacttgt gtgagaggta attttaataa ccttgagaga gggcttctca tcgctgtgat
     6361 tccatgacca agtgtttcgg aacaatagag taggtaaaag atgtaaagtc gcctcctcgg
     6421 gtcctcgatt tataatgcgt atttgaatga caatatcatc cggtagattc tttgcatact
     6481 caacaaacac atcaaagtat cgggctcctt caaacactcc tgtatcgaga agttcatatt
     6541 cgggctccat gcggcctcta cgtctattct ctgcaactag ttgtgagtat gggtaagcgc
     6601 gctgaggata cttgtaaagg tatttcatat aggagtgcga tggtgtatta tctaaataaa
     6661 agtaatattc cttaacatct tctccatgat tgccctcact acctgttaag ccgaaaagtc
     6721 tttctttaat aattggatcg ttaccattcc aaagagctat agcaaagcag agcctttgat
     6781 ggttgtcaga aatccctgct ataccatcct caccccacct gtaggctcga gaacgggctt
     6841 gctcatgtgt aaaatagtcc caggctgtgc catatggact ataatcctct cgtacagtcc
     6901 cccattgccg ttcactcaaa taaggacccc agcgtctcca ataggctttc cgattgcttt
     6961 cttcctctaa tcttttttct tcatgtgtca tgctgtctcc tcctcaattc gtatctagtg
     7021 gatcgtggtt aatgaatcga aaaatgagcc acgaatcacg agacacgaaa cactaatcac
     7081 gatccacgag tcacgaaatc cgtgccctgg gtcacgatat tggcgctact tcgcaaaaaa
     7141 ccttaatggc acccttagct tctgttaact tgtttataag atcctgataa ttttctagcc
     7201 ctttcacagg atgagtaaga aattttggta accatccagg ccactgaagc tctgcctgag
     7261 agaagtcttt aacccctatt tcaaaataat cacgattagc gttaacagtg ccgaccacta
     7321 ccttgttccc gagtacaaat cctaaattta tcatatctgc tggaatctcg acgcttcggc
     7381 caccacctgt cacacttgag agaactagca ctccgttttt tcccactata ttcattgcct
     7441 caaagacgat tggcgaatat cccgtggctt caaatatgag attatatggg ccgtgctctt
     7501 tcgcagactc tgtaagggtt atatccttag tactgtgata acaaccaccg atttctccaa
     7561 ttaaatctga atttaggtat ggcggctttg ttaatccaaa tgtgcagacc tcaagccctc
     7621 tcagacgaag ggccatggtc gccaagagcc caatcgttcc tgctcctagg actgctgcac
     7681 gctttggatg ccatattcga agccgcctct gaatttcata agcctgatat atcccttttt
     7741 caatcacact tgtaggttca agaagcacac ctacctcttt caatcctttt ggtacattga
     7801 tgatatattc aggtgcatct acatagtatt cggtaaggta cccgtggcgt agatttattc
     7861 ctctctcata gtaaacatca tctgttgtca tgtcatacat gccaatttga tcataagggg
     7921 aactgcccgg acgacgtaca gtggcaacaa cgtattcgcc agattgcaac tccgtaacgt
     7981 tcggtccaat ttcctcaaca attccaaagg attcgtgtcc taaaattaga taatcgtcac
     8041 caggcggaga cgcaccgtac tccgcagcgt taatttcttt atcagtcccg tcaacaccaa
     8101 cgcgtagtat ttttacaaac actccacgac catttggtac atcacttacc tttggttttg
     8161 gaacgtcgcg tagatgaact gagttaggct taccaggtat aaccgctatt gctttcattc
     8221 ttttatcctc caccacttct ttcacttaca gtaacattag acgcattatc ctttatagcg
     8281 gtttcaaaaa acctatattg accatccatt cgtctattta cactcgtata aacaggatga
     8341 tagtttctaa ttatccactt aggttcatac acgaaccgaa gaaccatcaa ttcatttctt
     8401 tctcttcttt cccatcacga tccgctctgc ttcaagccaa tcgtcaaggt cactgccgtg
     8461 tcctccccct cttttttcat atagctcgtg agcctttttt gcaatcattt cttggagcgt
     8521 tctttcatca agttccatct ttcttctagg tttcttttgg cttgaaactt tttctttggc
     8581 cattatatat gcctccttta tctttctctc tcacagttct aaaccgacgt tagaacatac
     8641 accttatagt tcattttcta agttcccatc ctaacctctg ttcccccaca cttaggacag
     8701 gtgtggacct cttcaagaat tcctccaagg tcagggtcca tcttatctgg gtgagttaat
     8761 gcggcagtgt aatcaatctt atcagcatgg tagttcatct ccacaccaca agagggacaa
     8821 atcatctttt tatgttttga tttaacgtct tttgccataa atcaccattg ttattttttg
     8881 taggggcgaa cctatttgtt cgcccattat cagggcagac acagtggtct gcccctacaa
     8941 cgggacgtta tctcgcccat atctcaaaac ccttgccatc cagatcaact ccttcaagaa
     9001 tttatcaatt cgcttaatat atgaatgatc caggagctta ccggatgaat cgaatatatt
     9061 ttggacgcta gagaagttcc cgtcccagaa tatggtaaca agtcccaact ccctcatcac
     9121 agggagtagg ttttgtatca ccctagtacc accgaaacct cctgcagata caccacagat
     9181 gccaacagcc ttatgtatat attcctttag gttagtatct agaacgtgct taagaagtcc
     9241 tgggtagcca tggttgtatt caggcgcaat aataattaaa gcgtctgcac gcatgacagt
     9301 ctcagagaat tttttattct taatgttttc tcccgcatct gaagttgtaa gtgagagatt
     9361 acgaatgtcg attaactcag tctctacacc atctcgctta gatacctctt ccaaaataaa
     9421 ttttgctacg tgttcactca tacgcccctg cctcgaggta ccaagaatta caggtataaa
     9481 aatcagtctg tttgaattca taatatcact ctttcactag ctaattattt ttccacaatt
     9541 aatatcgcat ggccgtcggg gtcgaaaact tttatcccat tcttataccc gagtttaccg
     9601 ttaggcaacg tactaacctg atcagagaca aaccggactt tcctattgcg aagccattcc
     9661 tctgccattt ccacgtcatc aactatcaaa atagtttgcc aatgcacaat atcgttggcc
     9721 tttatttctg atggaatagg tcggccattt tcaggagaca cgtactctaa aaactcaata
     9781 ccaggtatgt ttgcagaagg tcggagtgaa gtaatacgaa ccttagcgcc ggaaacgtta
     9841 tccaggttat cctgttcggt gccgatattg aggctccctc ctagtacatt catccccaat
     9901 agatctctat aaaacctaag acttgcctct gtgtcattta cgccgattgc cgtatgatca
     9961 atccccagaa atagcttatc agtaggacgg tgccatcggg gatcgccttt gccaggcgga
    10021 aaccataaga gttccaaggg atgactgtct gggtctttaa acttgaacgc cctgatccct
    10081 gccgctggtt tattagattt tggaagtgtc tgtgggctag tcgagatttg actcaccttg
    10141 tgcttgttta catgctgata tgccttgtcc atatcactca caacaatagc aaagtgctga
    10201 aaccataggt cattactccg agaatcaacc ggtattggct tgccgtcgga gggctctata
    10261 tattggatca agctaatgaa ctcatctccg atttgcatac gaacaaagcg tatccgaagg
    10321 tctggtaaac ctagaagctc ttcataccct tcaccctcca ctacaatatc caataccttt
    10381 ttgaacgata aaacattcga ataaaaatca actgtgcgat ccatatctga gacggttata
    10441 cctatagcat ctacagccat aactttaggt tgttgacttg gctgttgtga cagcgctgtt
    10501 tgcgccaata accctattac taaaagtaac caaagaggaa gaaacgtttg tttaattctc
    10561 atgctgattc tcattattta acctttctac tcacctgagt cgctcaagca aatgatcgcc
    10621 gactcggagg gcgtttgcga taattgtaag tgcaggattc actgcgccgc ttgatgggaa
    10681 gaagctagcg tcaacaacat agagattatc caaatcatgc gccttgcaat tcacatctaa
    10741 cgctgaggtt ttaggatcac gtccaaaacg gatcgtaccg ttctgatgtg ccacgccggc
    10801 gagcgggatt cgctggccta cgaataagtt ccgtgaaaac aacccttggt ggcaatcgtg
    10861 tccatgttcc gggcatttag tctgttgctt catcaagctc tcgagcttct tgatcagacg
    10921 cttgtgcccc tcttcgttat tctgtttata ggcaaggacg atattaccct cgctgtcaag
    10981 cgtaacacga ttctctggat ccggcaggtc ttcagatgta agccagaagt cgagtgagtg
    11041 tttggctatg tattcaagtg tccaacccgg tgttagtgct ggcgcaccag ctttaagtgt
    11101 ttctgcatcc agcttgccaa caaaggaaat gtgacccatt ggatattccc attccttcga
    11161 agcgaagtag aagtcgttaa ctgaaaggga cttttggaag attgtagggt taggacacct
    11221 tgaaatcgcc ataagtaccg agttgacgtg acccatgtag tggcggccaa ctacgtctga
    11281 gctatttgct aagccatgtg ggtgcttgtc gttcgcagaa cgtaaaagta atgctgcgga
    11341 atttatcgcg ccacaggaaa tgacaactat attggcggaa taagtctcct tagcaccgtt
    11401 tcgctctaca taaaccttag ttacctcagt tacctctttt ccagatgcac tcgtctctat
    11461 gcgagaaaca aaagcatggg tgagtaacgt aacattcgga tacttaagag caggatcgac
    11521 gcagagaaca tgtgcatccg actttgcata cactaaacag gggaaaccat cacatgtatt
    11581 gcagcgtata catcggctat tgtgcatgtt cttttcatcc agcatgacgc caaggggtgt
    11641 gtgaaacgga cggaggccga ggtgtgcgaa gtcgtcactc aactgctgga tgcgaggctc
    11701 gtgactaacg ggcgggtaga gataaggcgc acccgtcggc ggctcggtgg gatcgacccc
    11761 ccgttgacca tgaacctgat aaaggtgctc tgccttagtg taatatggct ccaactcatc
    11821 gtagcttatg ggccacgcag gtgagacgcc accatgatgc ctaatttcac caaaatcttc
    11881 tttgcgaagg cgaaacagcg ctgcaccgta gaatttggta tttccaccta cgtagtagtt
    11941 cgtatgagga tgaagcggct tcccttcttt gtcacgccat acttctttgg tttgatattt
    12001 tccctcaacg tttacggcac gtggctccca gttgtccttc tcacgcggga cataatctcc
    12061 acgttctagt aagagtatac gctttccaga aggagctaga tgataagcta gggtgccacc
    12121 tccagcccct gaacctatga taattacgtc ataatgggta tcttgaggca tgtagcttaa
    12181 ctcccttggt gttgctgtgg tgtcaatggc gcagtgccga aattaacacc gaaacgctga
    12241 caccatatag taacagcttt gtatttactc aaatctacat cggcaggcac atcatagttt
    12301 tgatctccga tgttaccttt aagcgagcct aacgacacaa agccagcctg tttaacagta
    12361 tcattgtcat tggcatcttg agctgctact agataaacct gaacatcagg accattagat
    12421 gtctcaaact ctgtaaatcg aagaaccctc ttgccatccg gcaattgata aattgttgct
    12481 gttcctttac catcgtgggc tactccatga aaattaccag tgacaagaac tactggtgag
    12541 ctttttgatc ctgtcgtctc ttgggcagta attggaaatt cttcttgtac tgtctcactt
    12601 atatagagct tttctgggcg aaataaatac caaagaacca caatgattat taatatcaca
    12661 ataattgcaa ttgtgcgttt catgatgcat tacctccttg tcttttttaa tgatcgccag
    12721 ctagtagcca accatattgc tgattttata caacttatga ccttactaga ctcattttct
    12781 attgctaacc tatagatact ttccactaag ctattgcatt tctaatctct gtgactttcc
    12841 gaacaaccta ccttacacct agaaagcttt ttcacagcta attagacgtt agaattttta
    12901 gaaaagattt aaatcaattt tttattattg attgggtcta agaaatcaca gaacagctgg
    12961 aatctgaacc ccatcattat tattattaaa aaatatttga atgatgaagt caatagatgg
    13021 tagaccctgt aacttagcta aaattgtata aaagtattat ctttacaaaa tgcaaaatag
    13081 aagttaatga acttgagtta aaaattttta tgagttcttt gaagttctta cttcgatttg
    13141 cattccttaa atgtttattc cctcctaaaa acccttccct ttcgtattta gcttaattct
    13201 gtagattgag tcactagctg taatatataa aactgaacca tcgtttcccc atccaacatt
    13261 cgaggtggga acacctgtta ctatagtgcc taaatgcgtt ccatccggtg aaaatacgta
    13321 cacaccgcct ggtcctgcgg caaaaaggtt accatttatg tcaaccttaa aaccatctgg
    13381 ggcacctttc attctcttag taaaaggcat agcatcataa aaaacgcgcc catttgtaat
    13441 cgttccgtca tcctttacat caaatgcaag ccaagcagac ctctttggat caacatccgt
    13501 aacgtataaa attttctcat ccggagagaa ggcaatacca tttggggctt tgatgtcttt
    13561 ggtaagtaac gtgagcttgt catcagaaga aagccggtaa acgccctgaa acggaagctc
    13621 tttagctgga tcgttgaatg tctctggtaa tccgaaagga ggatctgtaa agtaaagatc
    13681 gccatttgac ttatacactg cgtcgttagg actgttaaga cgtttcccct cataacgatc
    13741 tgcaagcgtt gtcttagtac cgttcttctc gaggcgagag acacgtcggt caccatgttc
    13801 acatagcacg agcatgccag aggaatcgaa tagaagacca tttgaaccag gctctttacc
    13861 cttgaatagc gcagatcctg tataaccgct aggcttcaaa tataaactaa ccccctctcc
    13921 ttccttccat ttgagaacag aattacttgg gatttccgaa aacaacaaat agccgccttc
    13981 ccgattccaa acaggtcctt caacccaggt gaacccttct gccaacttct ctagtaccgc
    14041 accctgtgga aacagttgat ttaatcgcgg gtctagacgt acaatcgtac cggaagtagc
    14101 tgttgagcta ttcacctctt ctgccctcgc tagaacgatt tgaagaatta taacaattat
    14161 aaccatagcc tgtatgaaat tcagaaagcc actgcttaaa atctttcttt ttaccttcat
    14221 aactttctcc tccaccgtct atgccagtga taccaataag cacactacgg ctcactggca
    14281 cttctttcct cttagcgagt attgcttcta cagcatgatt aatgacatat caccttataa
    14341 ttaaaatttt ctttatcttt acttccaaaa cttcttacga atctctctaa ttagtcgagg
    14401 gtcgaacgcc aggtatgttc aattgcatcc ggtataaacc actccgagca cagagatata
    14461 gagtctttcc atcttctcct ccccaggcca tattgtgaat atgcttagga gcaactatcg
    14521 ttccaatgtg ctttccttct ggtgaaataa cccaaagtcc acccggtccc gaaacatata
    14581 tgtttccttg ctgatccacc ttaactccgt caatcgcatc ctcccctggt gcacttgtca
    14641 tatcaaagaa gatttttccg ttagacaatg ctccatcggc atttacctca tacctcatca
    14701 caaccttcgt ttctgggtcc cagttgccca tgtaaaggaa tttttcatct ggtgagaatg
    14761 ctatgccatt aggtcccttc aagtccttaa tgagcagtgt tagttttcca tctttaaaag
    14821 aatacacccc gctgaagggt agctcctttc gcggatcatc aaagaacttt ggcaacccaa
    14881 aggggggatc tgtgaagtag agggtgccat cagacttata tacgagatcg tttgggctat
    14941 tgaggcgttt gccctcgtaa ctgtctacaa gtacagtaag tgccccatcc ttttccagtc
    15001 gggaaacgcg gtggttaccg tgttcatcaa ttgtcagcct accctgagcg tctaacgtaa
    15061 gtccattaga tccaggctgc ccgtactcag caacatcagc accagaataa ccgctaggtt
    15121 gacgaaatac cgaaagcatc ccgtcctttg tgtatttgta aataatgttg ctgttcgggt
    15181 cactgaagag cagatagcct tcattactaa cccatattgg accttctgtg aacttgaatc
    15241 cctcagcgag tttaaaaatc ttggggtttg gtccgactat ggcatccatc gcggaatcaa
    15301 gccttgcaac ctctacgttt acctcttgtg gtgtcactgc aatcggtacc ttactaccct
    15361 tatagaaatc aagccttgca tagcgcatcc aaataaagtt tgtgggtgga ttagaaatcg
    15421 gtccgttgat tccgaaaata gcaagttgaa tcttctgttc aggccttaca tcgcgtccaa
    15481 ctacaatgcg attagaggca ttccaacctg caattacaga gccgccgctc tgaccgagtg
    15541 ctcgtggaat ttcaccatcc acccaaatct cagcgtaatc gtcaagcgaa gtctcaaaca
    15601 caactgttga ccgtgttggg tcaaagttgc ctatacgttt aggaattgct atgttgattc
    15661 tataccaatt gaagcaaatc ctgcctgttg aacgtcgctt attaagtgaa gttggatcca
    15721 aaacctccca ttttgaatca tcgaaatcca caccacccgc atgcggctta taatcataag
    15781 tcttaactgg tttacccgta ggctgcccat ccggtccagg cgctttaaaa tcaacctgta
    15841 tgatttcaac atcgctataa cgccattgac ctttcacaag attaacccct tccttcgtgc
    15901 ttagatcaat agacgcatta ggtataccac ttggtacgtc ctgatttgtt tgtgcgagca
    15961 cacctattac acaaagaaaa gagactagta tagaaaacaa aattaaatga atcgaacggg
    16021 atgtcatttg tcaccccctt taattacggc atcaatagct gaagccccaa cctttgcaat
    16081 atcttcgtcc tcttgtggag tttctccgct gacaccaatt gctcctataa cctttccatc
    16141 ataaataatg ggaatcccac tctggagagg cgttgcccct ggtagcccta atgcagcaac
    16201 acgcccctcg cgaacctgat cctcaaacac tttgctcgga cggcgaaata tggcagcggt
    16261 tcgtgccttc cctataccga cttcgacact tgccacctgt gtgttatcaa gcctctcgag
    16321 gagaactaaa taacccccat catcaacgac cacaatcacg actttcgcac ctctttttat
    16381 cgcctctgcc tcagccgctt cagcaatctt ttttgctact tttaatgtca gcacatgttt
    16441 tgatggtaat tcagccagga tagaaggtgc caatagcaat aataaaagaa ctaaacttat
    16501 tataaatttc atttcaacct cctcctctta actcctcaca gcgaatttat taacacaacc
    16561 tggatattaa aatcagggta gaccgccagt cccctcgacg agacccttca atgctttcag
    16621 gccttagact tgggacaggc ttgctggtgg cctaagacgc aacgagcaag actcgttgcc
    16681 tacccaattc taatctctaa taccaaaaaa aataattcaa agaccattca ttcatgattg
    16741 tacactcccg attaaactta tcattccctt tgaagttgcc ttagctaacc ttaaccacat
    16801 aataaagcga aggtgcctga actgctttga accaatgcgg cgtgcctgct ggcacgacaa
    16861 ttacatcacc ctttgtaatc gtacgggtct ctccaccgct tatttccttt cccctgattt
    16921 catcttgagc agtagtttta ccatcaacca cagttccacc tgtaaccaat gtggcagaac
    16981 cttcaagcat ataaattatg tctgtatctt taacatgcac ctcagccata ccaggttcag
    17041 tacggcgact tgcatgaatc ttgtagttag ctatttcaag caacggcatc ccctttgcaa
    17101 acgcagccga gactttacta ttgtcaaaat aggtaacctg tgcagatggg gcagtcatca
    17161 tactggctga agaactacct attgcattgg ctccggctac tgcgatctct tcatcttgct
    17221 gggcactagc agcgccgctt accccgatag caccaacgat ttcccctcca agcgtgatag
    17281 gaacaccgcc ctgcagtggg gtaaaatcct gaagggcaac catcgatgtt ctgcctttag
    17341 ctataatgtc ttcaaacacc tttgttggct ttttaaaaag cgctgcggtt cttgcttttc
    17401 ctattgatat atttgcacca gccgcaaatg tgccatcaag ccgctcaaga gcgatcagat
    17461 ttccaccttc atctacaact gctatagcag ccccgggagc attgtttttc tttgcatagt
    17521 ccacagcagt agctattacc ttcatagccc cctcaagtgt aagtgacttc ttgtttgcta
    17581 cctgagcatg gctagcggta gacataatca aaaggacaaa cactataaca aaatttttaa
    17641 gattcataat tttccctcct attagttgat ttattcccaa ttgatcagct catcgcccct
    17701 ttccaacaca ttcagtttag atagagggat gggaggcggt taaattagcc ctaattcaag
    17761 ctgcacccac aaaaaaccag ctacatcacc ggtgacgcaa aggaagcttc acccagtaag
    17821 gtctcagcaa aggcacacca gatttgtacc ttcacaacat ctggaatata agacggcaaa
    17881 gttatactct tgttaacctt tccgcctttt atgtccaaac gctggagaag gtaaacattt
    17941 ccctttgaat caatcacctg ccagtgagga tcaggggtgc cgggaacctt aaaatcatca
    18001 gacaatgtca agacgttctt accatcctgt ttggtgtggg ttacagtccc accattcacc
    18061 ttcaccccct taaagaccgt actggtatgc atgtcagcag caaagctgct tatagtggct
    18121 aaaaataaca ccgcgatcaa agaactaaaa agacccattc ggatccttct gaatgtacca
    18181 aggttatcca tggtaatacc tcctataagt actttattta agtcaaaaac tctcatgatt
    18241 aaccctccct ttcctttagt ctcaagaagc tgttttcact gcccccggat acccacaatg
    18301 tatgcaacgt ttatcatttt gatggtggag cttagcacaa aatatattct tcggacctct
    18361 taaacggagg taagcctctg ctgctaatag cattcctata ggtggagcgg tgaagtagat
    18421 ccataaaaaa ttccaaagct gtgcatgaaa ggcggagcca aaacttcgtg ctggattcat
    18481 gctcattccc gaaataggcg cttctaaaga gatataaacc gcaaccaata caccggcgaa
    18541 aagccctgtg taccttgcaa tatcattagt attagacact ataaggatta caaacataag
    18601 aataaaagag ataataagtt cagccagaaa tgctatgccg ttcccaacca taccaggtaa
    18661 agtcgcaaca taattcacag agggatggga gattagctga cccatcaaag tagatgctat
    18721 cagaacacct ataactgccc ctacgaactg tgcgataata tagaaaaccg catcccagaa
    18781 cttaacctta ccaagacgaa agaatgtcaa cgtaacagaa gggtttatat gagcccccga
    18841 ttgctttccc caaggcgaat aaatgatacc gactgcggtg agacccatcg ccattcccat
    18901 cagaacacgt cgtagaaaag ggtctggtaa tgcctgatgt aaaggcgaat ccggatgctc
    18961 aagaacgaat ccaaaaaagc aagctgaaat cataaataag ccaagtccag aagcttctat
    19021 aaaatattca ggccagtgct gacttaaggc ttttatcatt gtaagctttt cagtgttagt
    19081 ttgagtaacc gccattgtat ccattatgcg tacattagac gttagaattt ttcagaaaga
    19141 tttaaacaaa ctagttctaa gttagatgtt atcacaatag cgtttactaa tttaagttcc
    19201 aggaattgat cttaacaaga cggggattgt tcttaaacta ttggtatatc atcttgacct
    19261 ttaaagtgaa gagtataagg tataaaccct agaaatgaat tatttgccaa agtttgacat
    19321 cagagacgtg atgtataaag tatatggaga ttaaacaaca aataatttgg tacatgcgcc
    19381 gaagagccca gataataact agaattcaag aattgatatt cttgatctca tctatagaat
    19441 gtgaacatat tataaaaata catccgatct ctatatcaaa ttcggtaaat taaaccgata
    19501 gcaaaaattt tgtaatttga gattttaaca tcaaattatc tattcaaaat tg
//
LOCUS       DATDRL010000292        13683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_25320, whole genome shotgun sequence.
ACCESSION   DATDRL010000292 DATDRL010000000
VERSION     DATDRL010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13683
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25320"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2378
                     /locus_tag="VI935_09850"
     CDS             <1..2378
                     /locus_tag="VI935_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HLE25936.1"
                     /translation="EDKITNEIKEKEKYYAEVIRESEELRAKTETYRRLTSDKEDTLR
                     RFSRLKEDLHNLVKEIEKDKEIISKEKEILQHYNELENVREEERIFSEKLITYTNLIK
                     ELEKTRRSINDERSKIENEISSLSGRNGELENQLTQTMKLLKREEEIKAGFKEFLDID
                     FLEKEIENKRLVVEKLKSRERELENSIQRIRFEIGVQVKGLEAKTKELSEKTEKREGF
                     SEECEKLRAEIRVGEEIQTKYEGLKAILKEIGGEKKASISRKSEFEKREKEEMEKLNL
                     IKSQADKPQCPLCESPLGEEAREALIQKLEKSLLDLEDTLEEVNKNIGQLEKEEKRIA
                     LEIKAAELKIKNLQELNKKLGEKEKSLEDAISISKDLELAKNELDRLKNIIEKEDFGT
                     EFRYELKEITHEIRKTAYDERQHQEIKDKLESLRGIQTENKLLEDAKSNKDEIESQLV
                     SIKAKTLSLTKTLDEGLFAVKYKERALEIENQIRELRYDEQKHKELKSILKSLERFAK
                     EKESLDKAKLSLSLREKELENLEGRLTTERVRLEKTEKELKELEEVMTQSEALEKDKI
                     VLEDAISRLKSTKDKIVQEKSRVISKLEWITSLENKKEDALKKAKKLGYDMALYQELD
                     KAFGKNGIQALIIESAIPEIDIEANKLLSRLTDSGMTLSLEMVKPTQKGGEKETLEIK
                     VGDNFGTRSYETFSGGEAFRIDFALRIAISKFIANRSGAQLRTLVIDEGFGTQDKDGL
                     SQFVEAINTIKNDFDKILVITHIDELKDKFPVRIDVTKETGRGSSFEILYS"
     gene            2459..3061
                     /locus_tag="VI935_09855"
     CDS             2459..3061
                     /locus_tag="VI935_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HLE25937.1"
                     /translation="MNWFDVVILIVISIFALLGLKRGLIKEIISIVAIIVGILAGVLF
                     YPSLGELLIKTTLIKSKSLASICGFLLIMFFTFVLVKLIGFGFAKIIGTLHLNWFNRI
                     CGGCFGAIKGVIISFLIIAGLGFFLSEKEPPFSNSILVPYVKESFSILKEHIPDNFDN
                     NIQKAKKLIQEKGIKAAIKEVEKVKETYLEENRKEKEPKK"
     gene            3159..4169
                     /gene="fbp"
                     /locus_tag="VI935_09860"
     CDS             3159..4169
                     /gene="fbp"
                     /locus_tag="VI935_09860"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HLE25938.1"
                     /translation="MIGIETLDEFIIRKQEELPYAKVELSRLLRDIGLAAKVVNREVN
                     KAGLVDILGTTGATNVQGEEVKKLDVYANTRFIAALKLGGQCSGIASEENKDIIVFND
                     EISKNAKYVVAMDPLDGSSNIDVNVSVGTIFSIYRQVSSVGPPTLQDFLQKGSEQVAA
                     GYIVYGSSTMLVYTTGHGVNGFTLDPSIGEFCLSHPDMQTPKSGKIYSINQGYFVSFP
                     EPVKQYVRYCKQEDKATNRPYSLRYIGSMVADIHRNLISGGIFIYPPTKDAPRGKLRL
                     LYECNPISFIVEQAGGRASDGYKRILDIEPKELHQRTPIFIGSEEMVLKAEEFMSDYP
                     SA"
     gene            complement(4502..5587)
                     /locus_tag="VI935_09865"
     CDS             complement(4502..5587)
                     /locus_tag="VI935_09865"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HLE25939.1"
                     /translation="MAKAQTSAKGNIDWVLELLGNEADSILSHKCKTIPKEQLHLPGP
                     SFVDEILVPSDRHPAVLRNMQLLFNTGRLAGTGYLSILPVDQGIEHSAGASFAPNPIY
                     FDPENIIKLAIEGGCNAVASTLGVLGSVARRYAHKIPFIVKINHNELLTYPNKYDQIL
                     FGTVDQAFQMGAVSVGATIYFGSPESTRQIQEISKSFQYAHSLGLVTVLWAYLRNPAF
                     KVGDIDYHLSADLTGQANHLSATIEADIVKQKQAENNGGFKALKFGKTDERVYTELTS
                     DNPIDLTRYQVINCYMGRAGLINSGGESAGASDLAQAVRTAVINKRAGGMGLITGRKS
                     FQKPMKEGVELLNAVQDVYLNKDITVA"
     gene            complement(5611..6804)
                     /locus_tag="VI935_09870"
     CDS             complement(5611..6804)
                     /locus_tag="VI935_09870"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HLE25940.1"
                     /translation="MAKMVVSDLPDSTFEGRRVFVRVDFNVPIKDGIISEDYRIRRTI
                     PTIDYLIERGAKIILGSHLGRPRGMVIPDLSLRPVSLRLSELLGKHVSFIEYRLSDEV
                     KMSVYELQEGEVMLLENLRFHKEETENDLQFSKEIASLADIYVNDAFGTSHRKHASTY
                     GMAFNFDLRLAGFLVNRELKFLTRLRENPDHPYVVIVGGSKIKDKINALKNLLEKADK
                     VLIGGGVAYTFLKAKGVNVGGSITEDEMIDWVRESLEEYEEKIFLPIDHVVAESLEHR
                     KTCMVVDQEIPEHFRGYDIGPKTVNLYTSEIKGTGSIFWNGPMGVFEVSDFSAGTTHI
                     ARAIALATWRGGTTVIGGGDTIAAIRKAEVLDSEIVHISTGGGAFLEFLGGAELPGIS
                     VLCDK"
     gene            complement(6804..7811)
                     /gene="gap"
                     /locus_tag="VI935_09875"
     CDS             complement(6804..7811)
                     /gene="gap"
                     /locus_tag="VI935_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HLE25941.1"
                     /translation="MSIKVGINGFGRIGRNVLRIGVERKGLEVVGINDITDAKTLAHL
                     FKYDSIFGSYKGEVKSENSHVVIDGKKIRVFSEKEPARISWDDIGAEVVVEASGLFTS
                     REAASKHLHGTVKKVIITAPAKGEVDLTTVLGVNHNQYDHSKHHVLSNASCTTNCFAM
                     LVKVLHENFRIKRGEMSTIHSYTNDQRILDAPHKDLRRARAAALSIIPTSTGAARAIE
                     LIFPELKGKLSSVAMRIPTSDVSLVDFSCEVEKVTTAGEVNRRFKEASERELKGYLRY
                     VDEELVSVDFVGDTHSGIFDSSLTSVVDGTLVKVLGWYDNEYGYSSRVVDLIEFIGDK
                     F"
     gene            8032..9072
                     /gene="argC"
                     /locus_tag="VI935_09880"
     CDS             8032..9072
                     /gene="argC"
                     /locus_tag="VI935_09880"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HLE25942.1"
                     /translation="MEKKRVAVLGASGYTGSDLLRLLLSHPNVEVTYATAERHAGKTI
                     SDVLPHLRNLLKLELQPLDVKSIPDEIEIVFAALPHGASAKVVEELIERGKRVVDLGA
                     DFRLSHRVYKEWYGEHPCPELIPQAVYGITELFEPKIAKAKLVANPGCYPESAILGLA
                     PVLNNKMIEEDLIIIDSKSGVSGAGRSPELAYHFCEVNEGIKAYKVGEHRHTPEIDEV
                     LSNYSGLNVRVSFTPHLIPMDRGILSTIYIKLREERTTKELLDLYENFYIGKRFIRIT
                     PEKTYPSTGDVRGSNFCDIGIRANPKNGTAIIISVLDNLVKGASGQAIQNMNVMMGFE
                     ESAGLDMPPVFP"
     gene            complement(9121..10317)
                     /gene="nifS"
                     /locus_tag="VI935_09885"
     CDS             complement(9121..10317)
                     /gene="nifS"
                     /locus_tag="VI935_09885"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HLE25943.1"
                     /translation="MKRVYLDYNATTPIDPRVFEAMIPYMKEEFGNPSSIHSYGMAGK
                     AALDNSREQIAELLGAGPREIVFTSCGSESNNLAIKGIAFQLREKGNHLITTQVEHAS
                     VLETFRFLESQGFNSTYLSVDKYGLIDLEELREAITDDTILVSVMFANNETGVLMPIQ
                     EIAELVKEKRVIFHTDAIQAVGKIDINLKNLPVDLLSISGHKLYGPKGVGALLIRDGV
                     KFGPLIHGGGQERGKRSGTENVAGIVGLGKACEIFKQDAGYKMQDARIKDLRDELYEG
                     ISERISGFKLNGHHEKRLSNTLNLSFEGVEGESLVMNLDLEGIAVSTGSACSEGNVEP
                     SHVLLAMGLSKEQAVSSVRFSLGRFTEKEDINRVIDVLPGIVERIREISSSDKGNLPQ
                     SAQRVS"
     gene            complement(10326..10649)
                     /locus_tag="VI935_09890"
     CDS             complement(10326..10649)
                     /locus_tag="VI935_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLE25944.1"
                     /translation="MDIETLTNYERYRTNWKKYVSQIKSEVIKLCSDTEVYIFGSVIK
                     GNTHPTSDIDVLLVSVEFKNIKKRIETHSRLTLLFLDAPFEFHLIYPEKFPFYKKMAK
                     EMVKI"
     gene            complement(10684..11022)
                     /locus_tag="VI935_09895"
     CDS             complement(10684..11022)
                     /locus_tag="VI935_09895"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HLE25945.1"
                     /translation="MFEFLKDRGLRFNEEAKGDFERGYFDFCLLHTEQMVQLLLKYLI
                     AIKIDDFPRIHSISRLFKEVANLYGQGVMDFYRDNISIITALEEAYVGARYIDIEFDK
                     EIAAQVLDFG"
     gene            complement(11058..11753)
                     /gene="cysE"
                     /locus_tag="VI935_09900"
     CDS             complement(11058..11753)
                     /gene="cysE"
                     /locus_tag="VI935_09900"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HLE25946.1"
                     /translation="MEIFKEIRSDFNAVFERDPAARSSIEVIFAYPGFHAIFLHRIAH
                     WFWNNHLIFLARFLSHISRFLTGIEIHPGAKIGKGFFIDHGMGVVIGETSEIGDNVTI
                     YHGVTLGGTSFSRGKRHPTIESNVTIGAGAKILGPLTVGSNSKIGANSVVIVDVPPSS
                     TVVGVPGKTVLKEEFPVYPDLEHNKLPDPEERAIQSLLEQVKELEKRINSLENDLEKT
                     KEENRVLQVVKTK"
     gene            complement(12016..12696)
                     /locus_tag="VI935_09905"
     CDS             complement(12016..12696)
                     /locus_tag="VI935_09905"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HLE25947.1"
                     /translation="MNDSKKHMIIIAHRGASAYEPENTLRSFKRAIEMGADMIELDVR
                     LSRDRHLVIMHDNEVDRTTNGHGFVKEKTLSELKKLDAGMGESVPTLEEVIALLKGKA
                     GFVIELKDLGTEEGVISLIKEKDLIEHVFIVSFNQNLLKRIKDLESKVKTGLILLSSS
                     DPIRLAKECQADAVAPFHEFITSDLIEKAHKNNLKIITWTVDNQGEAESLRDAGIDGI
                     VTNKPDIL"
     gene            12781..13647
                     /locus_tag="VI935_09910"
     CDS             12781..13647
                     /locus_tag="VI935_09910"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HLE25948.1"
                     /translation="MDYIRLLRPHQWIKNLLIIAPPFFGGAFLADPTIFFKMVQAFFA
                     FSLASSTGYIINDLFDLRTDRLHPIKKSRPIASGSVSVGMSIILLLLTFILSMGLSLR
                     FGEGFILIIILYLILNISYSLFLQHIVIVDAISIALGFVFRIGAGGEASGIEVSSWLF
                     LTTFLLSLLLAFGKRRFELMSFDNSTSFRRVLAKYRMNFLDTALSIFATTAIVTYSLY
                     AVERGPRVFIVTVPFVCYGVLRYMYLVQTDTSGDPTEALLKDKWLFSCVFLWIIATAI
                     IIYFQHNPSILR"
BASE COUNT         4285 a         2816 c         2578 g         4004 t
ORIGIN      
        1 aagaggacaa aataacaaat gagattaaag aaaaagaaaa atactatgct gaagttatta
       61 gggaatcaga agaacttagg gcaaaaacgg aaacctacag aaggcttact agtgataaag
      121 aggatactct gagaaggttt agcagattga aagaagattt acataacttg gttaaagaaa
      181 tagaaaaaga taaagagata atatctaaag agaaagaaat tcttcagcat tacaatgagc
      241 ttgaaaatgt aagagaagaa gaaaggattt tttcagagaa gcttataacc tacacaaatt
      301 tgataaaaga actagagaaa accaggcgat caataaacga tgaaagatcc aaaatagaga
      361 atgaaataag ctcactaagt ggaagaaatg gagagcttga aaatcagctc actcaaacaa
      421 tgaagctatt aaaaagagaa gaagaaatta aagctggatt taaagaattt ctagacatcg
      481 attttctgga gaaagaaata gagaacaaga gactcgtcgt agaaaagctt aaatccagag
      541 aaagggagct tgaaaatagc atccagagaa tcaggtttga aataggggtt caggttaaag
      601 ggcttgaggc taaaactaaa gagctttctg agaaaacaga gaagagagaa gggtttagtg
      661 aagaatgtga gaagctgaga gccgagataa gggttggcga agagatacaa acaaagtatg
      721 agggcctaaa agcaatacta aaagaaatcg ggggggaaaa gaaggcttcg atttcaagaa
      781 aatcagagtt tgaaaaaaga gagaaagaag aaatggaaaa gcttaatctc attaaatccc
      841 aagccgataa acctcaatgt cctctatgtg aatccccgct tggagaagaa gctagggagg
      901 cacttataca aaagcttgaa aagtcccttc ttgatctgga ggacacattg gaagaagtga
      961 ataaaaatat tgggcaatta gaaaaagagg aaaaaagaat cgctttggag attaaagcag
     1021 cggagttaaa gataaaaaac cttcaggaat taaacaagaa actcggtgag aaagaaaaaa
     1081 gtcttgaaga tgcaatctct atttcaaaag atttagaatt agctaaaaat gaactagaca
     1141 gactcaaaaa tataatcgaa aaagaggatt ttggcaccga gttccgatat gagcttaaag
     1201 aaataaccca tgagataagg aagacagctt atgatgagag acaacatcaa gaaataaaag
     1261 acaaacttga atcattaaga ggtattcaaa ccgagaacaa acttcttgaa gatgctaaat
     1321 cgaataagga tgagattgag agccagcttg taagtattaa agctaaaact ctctcactta
     1381 ccaagaccct tgatgaaggt cttttcgctg taaagtataa agaaagggct ttggaaattg
     1441 aaaaccagat tagagaactc cgatatgacg agcaaaaaca taaagaatta aaatctattc
     1501 ttaagagtct ggagcgattt gcaaaagaaa aagagagtct tgataaagca aaattaagct
     1561 taagtctaag agaaaaagag ctggaaaatc ttgagggaag gttaacaacc gaaagggtac
     1621 gactcgaaaa aacagagaag gaattaaagg aacttgaaga agtgatgacc caaagcgaag
     1681 cgctagagaa agataaaata gttctagaag atgccatttc aagattaaaa agcactaagg
     1741 acaaaatagt tcaggaaaaa agtcgggtta ttagtaaatt ggaatggatt acaagccttg
     1801 aaaacaagaa agaagatgct ttaaagaaag ctaaaaaatt agggtacgat atggccttat
     1861 atcaagagct cgacaaggcc ttcggaaaaa acgggattca agctcttatt atcgaatcag
     1921 caattcctga aattgatatc gaagcgaaca agcttttatc taggcttaca gacagtggta
     1981 tgacacttag ccttgagatg gtaaaaccaa cccagaaagg tggagagaaa gagacgcttg
     2041 aaataaaagt gggtgataat tttggaacca gaagctatga aacattttca ggaggagaag
     2101 cattccgaat cgattttgca ctaagaatcg caatatcaaa gttcattgca aaccgctctg
     2161 gggctcagct tagaaccctt gtaattgatg aagggtttgg cacacaggat aaagatggac
     2221 tgagtcaatt tgtagaggct ataaacacta taaaaaacga cttcgataag attcttgtga
     2281 tcactcatat tgatgaacta aaggacaaat tcccggtaag gatagatgtc accaaagaaa
     2341 ccggaagagg ctccagcttt gaaattcttt attcataaag ctgtcagcta tcagccttaa
     2401 gcaaaagcaa ataaatcaaa acctgatagc tgattattga ctactgataa ctaataagat
     2461 gaactggttt gacgtagtaa ttttaatagt aatttcaatc ttcgcgctcc ttgggcttaa
     2521 acgcgggctt attaaggaga taatctcaat tgttgctatt attgtaggaa tcctagctgg
     2581 tgtactgttt tatccttctc ttggggaatt actgataaaa actaccctta ttaaaagcaa
     2641 gagtttagca agcatatgcg gatttcttct cattatgttt ttcacatttg tgttggttaa
     2701 gctaatcggg tttggatttg ccaaaattat aggcacctta cacctaaact ggtttaatag
     2761 aatatgtgga ggctgttttg gtgcgataaa gggagtcatt atatcttttc tcatcatagc
     2821 aggtctcggt ttctttttat cagaaaagga acctccattt agtaactcaa ttctagttcc
     2881 atatgttaaa gagtctttct caatccttaa agaacatatc cctgataact ttgataataa
     2941 tatacaaaag gcaaagaaat taattcaaga gaagggaata aaagcggcaa taaaggaggt
     3001 cgaaaaggtc aaagaaacct acttagaaga aaatcggaag gagaaagaac ctaagaagta
     3061 aaatcccttc tgctttcttt gcacaaagtt ttgccaattt aaaattttaa attaaaatta
     3121 taaaagtata gtgctgagac aactatacat ttcttaacat gattggtata gaaacgttag
     3181 acgagttcat tatcagaaag caggaggaat taccatacgc taaagtcgaa ttatctcgtc
     3241 tactgagaga cataggtcta gcggctaagg ttgttaatag ggaggtaaat aaagccgggt
     3301 tagtggatat tttaggcaca acaggcgcca caaatgtgca gggagaggag gtaaaaaagt
     3361 tagatgttta cgctaatact cgctttattg cagctttaaa actagggggg caatgttctg
     3421 gcattgcctc tgaagaaaat aaagatatca tcgtctttaa tgatgaaata tcaaaaaatg
     3481 caaaatacgt tgttgctatg gatccactag atggttcttc taatattgac gtcaatgtat
     3541 ctgtcggaac gatattctct atatacagac aggtttcatc agtgggtccc cctactttac
     3601 aagattttct ccagaaaggc tcagagcagg ttgcggcagg gtacatcgtt tacggttctt
     3661 caacaatgct tgtctatact acaggtcatg gagtaaatgg gtttacctta gacccttcaa
     3721 ttggtgaatt ttgtctatct catcccgaca tgcaaacacc aaagagcggt aagatatatt
     3781 cgattaatca ggggtacttt gtaagctttc ccgaacctgt aaaacagtat gttagatact
     3841 gtaagcaaga ggataaagcg acaaaccgtc catattcact gaggtacatt ggctcaatgg
     3901 tagcggatat tcatagaaac ttgatcagcg ggggtatatt tatttacccc ccgacaaaag
     3961 acgctccacg cgggaagtta agactacttt atgaatgcaa tcccatatct tttatagtag
     4021 aacaggctgg aggcagggca tcggatggat acaaacgtat cctggatatt gagcctaaag
     4081 aactacatca aagaacacct atcttcattg gttcagagga gatggtatta aaggcagagg
     4141 aatttatgag tgactaccct tctgcctgaa ctcacacgag tgttacatct ttacttagtt
     4201 cgattatatc gcagcggtcc ctactttatt gcacgaataa attcgtgcct acaatctaca
     4261 atttttcgta ggcacagact tgtctgtgct ttcctttctt acttctttat cacagctgga
     4321 agttgctcat acaaaaatcg ccatctggat tcccaattaa aacattcggg aatgaaaaaa
     4381 acctatggag acctaaaggt ctccgctaca tttaattttg cctcacatta ttgtagagac
     4441 acgccatggg gtgtctctac cttaacccta catgatctcg ctaatttggg cgaaagaatt
     4501 ttcacgccac ggtaatatct ttatttaaat acacatcctg aacagcgttg agtaattcta
     4561 caccttcttt catcggtttc tggaaggatt ttcgaccagt aattaatccc attccaccgg
     4621 cccttttatt tatcaccgct gttcgtaccg cttgagcaag atcgctcgcc cccgcagact
     4681 ccccgcctga attaattaaa ccagcccttc ccatataaca atttatgacc tgataccggg
     4741 ttaaatcaat tggattatct gaagttagtt ctgtatagac cctttcatcc gtctttccaa
     4801 attttaaggc tttaaatcca ccgttatttt cagcctgttt ttgtttcact atatcagctt
     4861 caattgttgc gcttaggtga ttagcctgtc cagttaaatc agcagaaaga tgataatcta
     4921 tatcaccaac cttaaaagca ggatttctta ggtatgccca gagtaccgtg actaatccta
     4981 agctatgggc atattgaaaa gacttagata tttcctgaat ttgacgagtg ctctcaggtg
     5041 aaccgaaata tattgttgca ccaacagaaa cagcccccat ctgaaatgct tgatccacag
     5101 tgccaaagag aatttgatcg tatttgtttg ggtaagtaag taattcatta tggttaattt
     5161 tgacaataaa tgggatctta tgagcatacc tccttgcaac tgatcctaag acgccaagtg
     5221 tagaagcgac agcgttacaa cctccttcaa tcgctaattt aataatgttc tctggatcaa
     5281 aataaattgg atttggagca aaagaagccc ccgcagaatg ctctattccc tgatcaactg
     5341 gtaggataga taggtatcca gtacctgcca agcggccggt attgaaaagt aattgcatat
     5401 tcctcaagac cgcaggatgg cggtccgacg gaaccaaaat ctcatcaaca aaagaaggtc
     5461 ctggaagatg caactgctct tttggaattg ttttacattt gtgacttaag atagagtctg
     5521 cttcattccc caataactca agaacccaat ctatatttcc ttttgcagat gtttgagctt
     5581 ttgccatttt tctatccccc ttttaccttt ttatttatca caaagcactg agatcccggg
     5641 aagctcagca ccgccaagaa attcaaggaa agctccacca ccggtagaaa tatgaacaat
     5701 ttcagaatcc aatacctcag cctttcgtat ggcagcaata gtgtctccac ctccgataac
     5761 agtcgtaccg ccacgccatg tagcaagcgc aatcgcgcgt gctatgtgtg ttgtgcctgc
     5821 cgaaaaatca ctcacttcaa agacccccat ggggccgttc cagaaaatag aacccgtgcc
     5881 cttaatctct gatgtgtata gattcaccgt tttagggccg atgtcgtaac ctctaaaatg
     5941 ttccgggatt tcctgatcca caaccatgca cgtttttcta tgttcaagac tctctgcaac
     6001 cacatggtca ataggaagaa agatcttttc ttcgtattct tctaatgatt cccttaccca
     6061 atcaatcatt tcatcttctg ttatagagcc ccccacattc acccccttag cttttaggaa
     6121 tgtataagca actccgccac caataagaac cttgtcagcc ttctctagta gattctttag
     6181 tgcgtttatt ttatccttaa ttttagaacc tcctactatt acaacatatg ggtgatcagg
     6241 gttttcacgt agcctagtaa gaaattttaa ctccctatta actagaaatc cagccagcct
     6301 caaatcaaag ttaaacgcca ttccgtaagt tgaggcgtgt tttctatgag atgtaccaaa
     6361 agcatcgttt acgtaaatat ccgcaagtga tgcaatctcc tttgaaaact gcaggtcgtt
     6421 ttctgtttct tccttatgaa atcttagatt ctctagaagc attacctcac cttcttgaag
     6481 ctcgtaaaca gacattttca cttcatcgct tagtctgtat tctatgaaac taacatgctt
     6541 tccaagaagc tcagaaaggc gtaaggaaac aggtctcaaa gaaagatcag ggattaccat
     6601 ccctcttggc cttccaaggt gggagccaag gattatcttt gcccctcttt ctataagata
     6661 atcaatggtt gggattgttc ttctaattct ataatcttcg cttattattc cgtctttaat
     6721 tggtacatta aagtcaaccc tcacaaacac tcgcctccct tcaaacgttg aatcaggaag
     6781 atcggatacc accatttttg ccattaaaac ttatctccaa tgaattcgat caggtctact
     6841 accctagatg agtatccata ttcgttatca taccatccta gaaccttaac aagtgtgcca
     6901 tccacaacgg aggtaaggga tgaatcaaat atcccagaat gtgtatcgcc cacaaaatcc
     6961 acagaaacga gctcttcatc tacgtatctc aaatagcctt taagctcccg ctcagaggct
     7021 tctttaaatc ttctgttaac ctcgccagca gttgtaacct tttctacctc acatgaaaag
     7081 tcaacgagcg atacatctga agtaggaatc ctcatcgcca ctgagctcaa tttgcctttt
     7141 aattcaggaa atatcaactc aatcgccctt gcagccccag tgctagtagg aattatagat
     7201 aatgctgcag cacgcgccct tcttaaatcc ttgtgtggag cgtcaagtat tctctgatca
     7261 tttgtgtagg aatgaatcgt agacatctct cctcttttaa tcctgaaatt ctcatgtaat
     7321 accttgacaa gcattgcaaa acagttcgta gtgcaggagg cgttagataa aacgtggtgt
     7381 tttgaatgat cgtattgatt gtgatttaca cctaaaacag tggtaaggtc aacctcacct
     7441 ttagcaggtg ctgtgattat caccttcttt accgtcccat gtaggtgttt tgatgcggct
     7501 tcccttgagg taaatagccc acttgcctca acaacaacct ccgcacctat atcatcccaa
     7561 gaaatccttg ctggttcttt ttccgaaaag acccttatct tcttcccgtc tattacaaca
     7621 tgactgtttt ccgacttaac ctcgccttta taggaaccaa aaatagagtc atatttaaaa
     7681 agatgagcaa gtgttttagc atcagtaata tcgtttatac ctacaacttc taatcctttt
     7741 ctctcaaccc cgattcggag tacgtttcta ccaattcttc caaagccatt aatgcctacc
     7801 ttaattgaca tcgtattcct ccaagatcat ttaacttcag aatggtattt atattttaca
     7861 ttacatattt tgacccctag agtcaaggtt taataaggcg taagcctaat caaaacttgc
     7921 atttacccca attaccagat actttttatt tccatccgat gcacactgtt tattacctcc
     7981 cgaaccaaac tgcggttcgg gtaatacagc atcggatact actcaaatcc catggagaag
     8041 aaaagggttg cggtgcttgg agcaagtggc tacactggga gtgatctttt aaggctactt
     8101 cttagtcatc cgaatgtcga agtaacatac gcaactgccg agaggcatgc tgggaaaaca
     8161 atctcagatg ttcttcccca cttaagaaat ctcttaaaac ttgagcttca accacttgat
     8221 gtgaaatcga tacccgatga gatcgagatt gtctttgccg cacttcctca cggagcatca
     8281 gcaaaggtag ttgaagaact catagagaga ggaaagaggg ttgtagatct gggtgcagat
     8341 ttcaggctca gccaccgtgt ctacaaggaa tggtatggtg aacatccgtg tccagagcta
     8401 atccctcaag cagtttacgg aataacagag ctttttgaac ccaagattgc aaaagcaaaa
     8461 ctcgtcgcaa accccggatg ttatccagaa tctgcaattc taggacttgc cccagttctt
     8521 aataacaaaa tgatagaaga ggatttaatt ataattgatt ctaagtcagg agtttctggt
     8581 gcaggaaggt caccggagct tgcttaccat ttctgtgagg tgaatgaagg tataaaagca
     8641 tataaggttg gagagcaccg acacacacct gagattgacg aggtgctttc aaattattca
     8701 ggacttaacg taagggtttc atttacacca cacttgatcc caatggatag aggaatcctt
     8761 tctacgattt acataaaact aagagaagaa agaacgacaa aagagctact cgatctttac
     8821 gaaaacttct atataggaaa aaggttcata agaattacac ctgaaaaaac ctacccatcc
     8881 acaggagacg ttaggggctc aaacttctgt gacataggga taagggcaaa tccaaagaat
     8941 ggaactgcaa ttataatctc agttttggat aacctcgtta aaggtgcctc tggtcaggct
     9001 attcaaaata tgaacgtgat gatgggattt gaggaatccg cagggcttga catgccaccg
     9061 gtgtttcctt gattcggaat aaaaacagcc acagagaaca cggagactag attttttata
     9121 ttaagaaact ctctgtgcac tctgtggcaa attgccttta tctgaactgc tgatctctct
     9181 tatcctctca acaatcccag gtagaacatc tataacccga tttatgtcct ctttctccgt
     9241 gaatctccca agactgaatc taaccgatga aacggcttgc tctttggata gacccattgc
     9301 aagtaggaca tgggaaggtt caacatttcc ttcggaacat gcagaaccag ttgatactgc
     9361 tataccttca aggtcaaggt tcattacgag tgattcaccc tcaacacctt caaagctcaa
     9421 atttaatgtg tttgaaagcc ttttctcatg atgaccgttt agtttgaatc cgcttattct
     9481 ttccgatatt ccctcgtaaa gctcatccct caaatctttt attcttgcat cctgcatctt
     9541 gtatcctgca tcctgtttaa aaatttcgca tgcctttcca agccccacaa ttccagccac
     9601 attctcagta ccggaacgtt ttcctctttc ctgtccccct ccgtgaataa gaggaccgaa
     9661 ttttactcca tctcttatca acagcgcccc tacccctttt ggcccatata gcttgtgacc
     9721 agaaatagat agaagatcga cagggagatt cttcaaattt atatcaatct tccctacagc
     9781 ctggatagcg tcggtatgaa aaataaccct cttttctttc acaagctctg caatttcttg
     9841 tataggcatt aacacgccag tttcgttatt tgcaaacata accgaaacga gaattgtatc
     9901 gtcggttatc gcttctctaa gttcctctaa atcaattaga ccatatttgt ctacagaaag
     9961 ataagtggaa ttaaaccctt gtgattctag aaatctaaaa gtttcaagaa cggatgcatg
    10021 ctcaacctga gtggtaatta gatgattccc tttttctctt agttgaaagg caatcccctt
    10081 tatcgcaaga ttattgctct cacttccaca gcttgtaaaa acaatctctc tgggacccgc
    10141 tccgagaagc tcagctattt gttctcttga attatcaagc gcagcctttc ctgccattcc
    10201 ataagagtgg atgctcgaag gatttccaaa ctcctctttc atataaggaa tcatcgcctc
    10261 aaaaacccga ggatcaatag gtgtggttgc attgtaatct aggtagactc ttttcattag
    10321 aactactaaa tctttaccat ctcctttgcc atcttcttgt aaaaggggaa cttctccgga
    10381 tatatgagat gaaactcaaa gggagcatca agaaacaata gtgttaacct tgaatgggtc
    10441 tcgattctct tttttatatt tttaaactca accgaaacta aaagcacatc tatatcgctt
    10501 gttggatggg tgtttccttt aatgacggaa ccaaaaatat atacctctgt atctgagcaa
    10561 agctttataa cctcggattt tatctgagat acatatttct tccagtttgt tctataacgt
    10621 tcataatttg taagggtttc aatgtccacg agatcttatc tcctcaacca tcttcaaaaa
    10681 cctttaccca aaatctaaaa cctgtgctgc tatttcttta tcaaactcta tatcaatgta
    10741 tctcgcacca acgtatgcct cctcaagcgc tgttattata gagatattat ccctgtaaaa
    10801 gtccattacc ccctgtccat aaagatttgc tacttcttta aatagtcgtg aaattgaatg
    10861 gatgcgagga aagtcatcta tcttaatcgc tataagatac ttaagtagaa gctgtaccat
    10921 ctgttcagta tgtaacaggc aaaaatcaaa atagcccctc tcaaagtccc cctttgcttc
    10981 ctcattgaaa cgcagacctc tatccttgag aaattcgaac atgttttact tccgcgaatt
    11041 cctatataaa ttgtttttta ctttgttttt actacttgga gaactctatt ttcctctttg
    11101 gtcttttcca ggtcattttc tagtgagttg attcgctttt ctagctcctt tacctgctct
    11161 agaagggatt gtatagcacg ttcctcagga tcggggagtt tgttatgttc aaggtcggga
    11221 taaactggga attcctcttt aagaacagtt ttaccaggga cgcctacaac ggtagagctc
    11281 ggcggcacat ctactattac aactgagtta gcccctattt tactgtttga accaacagta
    11341 aggggaccca atattttggc acctgctcca atagtgacat tgctttcaat tgttggatgg
    11401 cgctttcctc tactgaagct agttccacct aatgtgaccc cgtgatagat cgtgacatta
    11461 tcaccgatct cagacgtctc tccaatcaca acccccattc cgtggtcaat aaaaaaaccc
    11521 tttccaattt tggcaccagg atgaatttct attccagtta gaaaacgcga aatgtgtgac
    11581 aaaaaccttg caagaaagat caaatggtta ttccagaacc agtgggcaat gcgatggaga
    11641 aatattgcat gaaacccagg gtaggcaaat attacttcaa tggagcttct agcggcgggg
    11701 tctctttcaa aaaccgcatt aaagtctgac ctaatctcct taaatatctc catttattct
    11761 gtggataaag ataaagacca aagctgctta agtcaagtaa gaaatatcga ttttgtgtca
    11821 gaatcattac tctaataata agtaacttac ctgggtaggc aacgagtccc cctcgatgtg
    11881 agctcgggac gggcttgctc tttctgccca ccagcaaccc ttcgacttcg ctcaggacag
    11941 gaactggtgg actaaccagt aggctatgct gttttcatca catgattaaa ttttgaaaga
    12001 ctcatcgata cacgcctaca atatatccgg tttattcgta actataccgt caattccagc
    12061 gtctctcagc gactcagcct ccccttgatt atcaaccgtc catgttatta tttttaagtt
    12121 atttttatga gccttttcta ttaaatcact tgttatgaat tcgtgaaaag gggcaaccgc
    12181 atctgcttga cattctttgg ctagccttat tggatcagaa gaggaaagaa gaatgagacc
    12241 tgtttttact ttggactcta aatcctttat tctttttagc aaattctgat tgaaagacac
    12301 aataaagaca tgttcaatta ggtctttctc ttttatcaaa cttattacgc cttcttctgt
    12361 tcctaggtct tttagttcaa ttacaaatcc tgctttcccc tttaaaagag cgattacctc
    12421 ctcgagtgtg ggaacgcttt ctcccattcc cgcatcgagc ttcttgagtt cgcttagcgt
    12481 tttttctttt acaaaaccgt gtccatttgt cgttctatct acctcattat cgtgcatgat
    12541 tacaagatgc ctgtcgcgag atagccttac atcaagctct atcatatctg ctcccatttc
    12601 tatcgctctc ttaaatgaac gtaatgtatt ttcaggctcg taagcgctcg cgccgcgatg
    12661 agctattatg atcatgtgct ttttagaatc gttcatataa ttaatatacc tagagacaat
    12721 aaaactcgtt gatataacta ctaaaaacag tagaatgata cccaaatgga aattcccttc
    12781 atggattata ttcgtcttct tagacctcat cagtggataa aaaacctgct cattatagct
    12841 cccccctttt tcggaggggc gtttctcgca gatccaacta ttttcttcaa aatggttcag
    12901 gcattctttg ctttttcact tgcttcctct acaggataca ttattaacga cctttttgac
    12961 ctgagaaccg accgacttca ccctattaaa aaatcccgcc caatagcttc tggaagtgta
    13021 agcgttggca tgtcaattat attgctttta ttaaccttta tcttatcaat ggggctttct
    13081 ttaagatttg gagaaggctt tattttaata ataattctct acctgatact taatatttca
    13141 tattcactct ttctacagca catagtgatt gtagacgcca ttagcatcgc tctggggttt
    13201 gtttttagaa tcggtgccgg aggggaggct tcggggatag aggtttcaag ctggctattt
    13261 cttacaacat ttcttctctc cctacttcta gcctttggga agagacgttt tgagcttatg
    13321 tcatttgata attcgacttc ttttagaagg gtacttgcta agtatagaat gaattttctc
    13381 gatactgcgc taagtatatt tgctactaca gcaattgtga cctattcgct atatgccgta
    13441 gaaagagggc ctagagtttt tattgttact gtcccatttg tgtgctatgg ggttcttcgt
    13501 tatatgtatc ttgttcagac agacactagc ggggatccta ccgaagcgct tcttaaagac
    13561 aaatggcttt ttagttgcgt gtttctttgg attatagcaa cagccataat tatttatttc
    13621 caacataacc caagcatctt gagataatcg ctgacgaagt ttaagacccg aaaatagaaa
    13681 ttt
//
LOCUS       DATDRL010000293         2538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_631693, whole genome shotgun sequence.
ACCESSION   DATDRL010000293 DATDRL010000000
VERSION     DATDRL010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631693"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /locus_tag="VI935_09915"
     CDS             <1..1581
                     /locus_tag="VI935_09915"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HLE25949.1"
                     /translation="IAFVRYPLSIDVVIKSIGFKASNIPVTLKDGDKIVSTEEISIDP
                     NDEKKVEFTLNPTSLGRKVYTVSIPVLADETIKENNQRSFVIDVIIDKVRVLHVAGSP
                     SWDVRFLRMALKRNPNVDLVSFFILREASDMVFASQNELSLIPFPVDELFGNELSSFD
                     VVIFHNFDFRPYGIYGLHLRSLRDYVMEEGGSFLMIGGDKSFDSGSYGGTPISEIIPV
                     ELNPIPPRSDETFSTESFHAKLTPIGLRHPVTRVIPNEKENEGYWKNMPELDGLNKVE
                     GLKPDAIPLLITPHGEPILVLSQVKSGRVASFLSDSFWEWVFVRAGEGELAPYYETFW
                     NRILLWLVSDPELKDLRVRAEKTSYDLGEEAKLDIWILGYENNQEKIEASVVFPDGIQ
                     RELNLEKISSDRFSAEVETDEYGVYKVKVKTRDGGLDNETDNIEDEIPFLVEPPINEI
                     KSPTIDQELLKNLAKKTGGRYITANDNPDGLGIDFSPKKIISGYKTREIWDKPWFFML
                     LVALLSSEWLLGRRWGLR"
     gene            1688..2452
                     /locus_tag="VI935_09920"
     CDS             1688..2452
                     /locus_tag="VI935_09920"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HLE25950.1"
                     /translation="MLLAVDVGNTNIAFGVFKGEEIINQWRIGTDKTKTSDEYGVLFR
                     NLLELKGVGSGSIKGAVICCVVPPLQRILISMVKSYFDVNPVLIEPGIKTGMSILYKD
                     PKEVGADRIVNAVAAFHEYKRSVIVVDFGTATTFDCISEKGEYLGGSIAPGLLISAEA
                     LFEAASKLPKVEITRPKTVIGKTTVQSIQSGLVYGYAGLVDGIVTRIKKEIGTNPKVI
                     ATGGLANLISPESLTIEEVDEFLTLRGLRLIYEWNK"
BASE COUNT          807 a          456 c          585 g          690 t
ORIGIN      
        1 attgcgtttg tgaggtatcc tctctccata gatgttgtta ttaaatccat agggtttaaa
       61 gcctcgaata tacctgtaac cctgaaagat ggagacaaga tagtctcgac tgaggagatt
      121 tcaatagatc ctaatgatga aaagaaagta gaatttacac tgaatccaac atcccttgga
      181 agaaaggtat atacagtatc tattccagtt ttagctgatg aaacaatcaa agaaaataac
      241 cagagatcgt ttgtgataga tgtaataatt gataaggttc gtgtcctaca tgtagcggga
      301 agcccttcat gggatgtaag gtttctaaga atggcattaa agagaaaccc aaatgttgac
      361 ctggtttcat ttttcatctt gagggaagca tctgatatgg tattcgcttc tcagaatgaa
      421 cttagtctga ttccattccc agttgatgaa ctttttggaa atgaactttc gagcttcgat
      481 gtcgtgatct ttcataattt cgactttcgc ccttacggaa tttatggttt acaccttaga
      541 agcttaagag attatgtaat ggaagaaggc gggtcatttc ttatgatagg gggagacaag
      601 agttttgata gcggaagcta tggtgggacc ccaatctccg aaatcattcc cgtagaactc
      661 aaccccattc cacccaggag tgacgaaacc tttagcacag agagttttca cgcaaagctt
      721 acgcctatcg gactccgcca tcctgttacg agggttatac ctaatgaaaa agaaaacgaa
      781 gggtactgga aaaatatgcc tgagcttgac ggattgaata aggtagaagg attaaaacca
      841 gatgctattc ccctcttaat aacaccccat ggagaaccaa tattagtcct aagtcaggtt
      901 aaatcaggaa gggttgcgtc ctttctttca gactccttct gggaatgggt tttcgttcga
      961 gctggagaag gagaactcgc accatattat gagacattct ggaatcgtat acttctttgg
     1021 ttggtgagcg acccggaact gaaggattta agagttagag cagaaaagac ttcctatgac
     1081 cttggcgaag aggctaaatt agatatctgg atacttgggt atgagaataa ccaggaaaaa
     1141 attgaggcct cagtcgtttt tccagacgga atccaaagag agctaaatct tgagaaaata
     1201 tcttctgata gattttctgc cgaggttgag acagatgaat acggggttta caaagtaaag
     1261 gttaagacaa gggacggggg gttggacaat gagacggata atattgaaga tgaaataccg
     1321 tttctcgtcg agccaccaat caatgagata aagagtccaa ctatagatca agaattactt
     1381 aaaaatcttg caaagaaaac gggtgggcgt tatatcactg ctaatgataa cccagacggg
     1441 cttggaattg atttctcccc aaaaaaaata atctcgggtt ataaaacaag agagatatgg
     1501 gataaaccct ggttttttat gttacttgtt gccctcctat cttctgagtg gttactagga
     1561 agaaggtggg gacttaggta attcgtggct cgtgtatcgt ggttcgtggc tcgtgtatcg
     1621 agccattcgg cagagctcaa gacgatgcca cgaactacaa acccgaatca cgaaacggag
     1681 aataaacgtg cttcttgcag ttgatgtcgg aaatacgaac atcgcatttg gggtttttaa
     1741 gggagaagag atcataaacc agtggagaat cggaacagat aaaactaaaa catcagatga
     1801 atatggtgtc ctttttagga acttattaga gcttaaagga gtaggttcag gctcaattaa
     1861 gggggctgtt atctgctgtg ttgttcctcc actacagaga atcctaatct ctatggttaa
     1921 atcctatttc gatgttaatc ctgttcttat agagccagga ataaaaaccg gcatgtctat
     1981 actttataaa gaccctaaag aggtaggagc cgatagaatt gtaaatgctg tagccgcctt
     2041 tcatgaatac aaaagaagcg tgatagtagt ggacttcggc acggctacaa catttgattg
     2101 catatctgaa aagggtgagt atcttggggg ttccatagca ccgggccttc ttatctccgc
     2161 tgaagccctt tttgaggccg catcaaaact acctaaggta gaaatcacaa gacctaaaac
     2221 agttatagga aaaactaccg tccagagtat tcaatccggt ctcgtctatg gatatgccgg
     2281 gctagtagac ggaattgtaa caaggataaa aaaagagata ggtacaaatc caaaggtgat
     2341 tgcaacaggc gggcttgcaa atctaatctc gccagagagt ttgactatag aggaggtgga
     2401 tgaatttctt acattaaggg gcctaagact catttatgaa tggaacaaat aggtttatta
     2461 tccacaaaca taagacaacc aatcctcact acgatttgag aattgagata gagggaattt
     2521 taaaatcgtg gattattc
//
LOCUS       DATDRL010000294         3855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1721763, whole genome shotgun sequence.
ACCESSION   DATDRL010000294 DATDRL010000000
VERSION     DATDRL010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721763"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VI935_09925"
     CDS             <1..821
                     /locus_tag="VI935_09925"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HLE25951.1"
                     /translation="YAAFPLPDRFTPRVFNKFISINEGMQVFGRQHGLTFVDVRNRFL
                     ELLSKGTPRAQYFASYTEAHPNPAGYLEIATLVANVFEPETEPSQYKSSDALKGIKEK
                     PEATQVEKTYKFLDNVGSPDTIIEKSDYEKVDITQFKINNDWREVLSEYPNSEVVFKD
                     VPVMENAELKFGVGINQNAWDKSGDGVLFEITVVDKKSQKNVIFSRYIDPKNNVEDRK
                     WFDINLSLKVFAGQKVSFIFKTTGGPKGDLNYDWAGWSSPQIILIGNKKKAANN"
     gene            929..1657
                     /locus_tag="VI935_09930"
     CDS             929..1657
                     /locus_tag="VI935_09930"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLE25952.1"
                     /translation="MNKNFLFWKLIWKLSANYMPEKLRKKIRWQIAHEDEKRFWLNLP
                     EVEFQAQAVGYRNLAEQILERLQARLKCGENFKALQIGCAVEDAIFYFPYGQLHAIDP
                     LANFYKANFKKSNNPKVDYRQASGEKIPFPNSYFDLIICQNVIDHVMDCNRVLNEIKR
                     TLKSNGIVYFGVDVYPPEIARLRGEYQIKNIIFDVQHPHTFTVESFEKLTDENSFNVI
                     ERYPMQPSGKGDESERYCVYVTQV"
     gene            1762..2676
                     /locus_tag="VI935_09935"
     CDS             1762..2676
                     /locus_tag="VI935_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLE25953.1"
                     /translation="MIVQILLSTYNGTKYIIPLMESLLGQDYPHMEILVRDDGSNDGT
                     VELLREYAANHTNIKVVTGVNLGFAKSFFKLIEISSPNAGYIALCDQDDVWLRDKVSR
                     AVEFLSRYPREIPALYYSRLAVVDENLKPLGYTKLPRKGLSFCNALVEGPEGSGCTIL
                     FNQAALQLLRTFPTRVYTHDWWIYLVVSGFGNIIYDKESRILYRQHDSNVFGIPFGVL
                     EKFRAKIHRFKTGQLQYQNAMDQAQEFMRIYGSSLPIEHKRTLEHLLENRKRFWLDRL
                     RYALSCEVYHQSTFEHFILKALIALNRL"
     gene            3066..>3855
                     /locus_tag="VI935_09940"
     CDS             3066..>3855
                     /locus_tag="VI935_09940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLE25954.1"
                     /translation="MPSNSNYISIIVPVYNNPQDLLECLSALRASYYPNSEIIVVDDA
                     STDDTHLIATRTGVRVLRLTENSGPAPARNHGARHAKGNILIFVDADVVVSPGVISRM
                     VKVFEEHPDVAAVFGSYDASPRADGVVSQYRNLLHHFVHQNGNPEASTFWAGFGAIRR
                     SVFEEIGGFDEKRFPKCSIEDIALGYYLRKAGYRILLDKSIQVTHLKRWTLRSVITAD
                     IFYRAVPWSKLILDEGNLPRDLNLQLSQRASSVLVSLLIIMLTLA"
BASE COUNT         1169 a          781 c          833 g         1072 t
ORIGIN      
        1 cctatgccgc gtttcctctg cccgacaggt tcacaccacg agtgtttaac aagtttatta
       61 gtatcaatga gggaatgcag gtgtttggtc gccagcacgg actgacattt gtggacgtcc
      121 ggaaccgttt tctcgaattg ctctccaaag gaacacctcg tgctcagtac tttgcgagct
      181 atacggaggc gcatcccaat cccgcaggtt accttgaaat cgcgacgctc gttgccaatg
      241 tattcgaacc cgaaacagaa cctagtcaat ataagtcttc tgatgccctc aaaggtataa
      301 aggaaaaacc agaagctacg caggttgaaa aaacatataa atttttagat aacgtgggaa
      361 gccctgatac catcattgag aagtcagatt atgaaaaggt agacattacg caatttaaaa
      421 ttaataatga ctggcgagag gttctatctg aatatccaaa ttcagaggtg gtatttaaag
      481 atgtcccagt catggaaaat gccgagttaa agtttggggt cgggataaac caaaatgcct
      541 gggacaaatc gggtgatggt gtgttgtttg agattactgt tgttgataaa aaatcacaga
      601 agaatgtaat cttttcaaga tatattgacc ctaagaataa tgtagaagac agaaagtggt
      661 ttgatatcaa tcttagtcta aaggtttttg ctggacaaaa ggtatctttc atatttaaaa
      721 ctactggtgg tcctaaagga gacttaaact acgactgggc tggatggagc agccctcaaa
      781 taatattgat aggaaacaaa aagaaggctg ccaataattg aacacaccac tttttgagga
      841 actttgggaa gacaactagc agatcaggac atttgaagaa actgtaatag aattagaaat
      901 aatttatatg gatcgaagag gtgaaataat gaataaaaat ttcctatttt ggaaattgat
      961 ttggaaatta tcagcaaact atatgccaga aaagctcaga aaaaaaatcc gatggcagat
     1021 agcacatgaa gacgagaaaa gattttggct aaatcttcct gaagtagaat ttcaagcaca
     1081 ggctgtaggt tataggaact tggctgaaca aatcttagag aggttgcaag ctcgtttaaa
     1141 atgtggggaa aattttaagg ccttgcagat aggctgtgct gtcgaagatg cgattttcta
     1201 ttttccttat gggcaattac acgcgataga ccctttagca aatttctata aggcaaattt
     1261 caagaaatcg aataatccta aagtagacta cagacaagca agtggagaaa agataccttt
     1321 ccccaacagc tattttgatc tcataatatg ccaaaatgtc attgaccatg tcatggattg
     1381 taaccgtgtt ctaaatgaaa taaaaagaac attgaaatca aatgggatag tatactttgg
     1441 tgtagatgtt tatccaccag agatagcaag gctgagagga gaatatcaaa taaaaaatat
     1501 catatttgat gtgcaacatc cacatacttt taccgttgaa agttttgaaa agttgaccga
     1561 tgaaaatagt ttcaatgtta tcgaaagata cccaatgcaa ccgtcgggga agggagatga
     1621 gtcagaaaga tattgtgttt atgtaacgca agtatagact tgagtggcaa atacgcaatt
     1681 cgagttttag ccccaacgat tccttatggt aagaggttct caggtatggc ggtgagtttg
     1741 taatgttgtg attaaggata aatgatcgtt caaatccttt tatccacata taacgggacc
     1801 aaatacatca tacctcttat ggaaagtctt ctgggtcaag actatcccca catggagatt
     1861 ctcgtgcgcg acgatggatc gaatgacggc acggtcgaac tgcttcggga atatgctgct
     1921 aatcatacga atattaaggt cgttactggc gtaaatcttg gatttgcaaa gagttttttt
     1981 aagcttattg agatttcctc gcctaacgcc ggctatattg cattgtgtga tcaagacgat
     2041 gtgtggctac gggacaaggt ttctcgagcg gttgaattcc taagccgcta cccacgagag
     2101 atcccagcac tttactactc acggctagca gtggtagatg aaaacttaaa accgttagga
     2161 tataccaaac ttccaagaaa aggattatca ttttgcaatg ccctagtcga gggtccagaa
     2221 gggtcaggct gtaccatcct ttttaatcag gcagcactcc agctactacg cacctttccc
     2281 acgcgggtat atactcatga ttggtggata tatctagttg tatcagggtt tggcaatata
     2341 atttatgata aagaatctag aatcctttat cgccaacacg attctaacgt cttcggaatc
     2401 ccatttggag tcttagaaaa attcagagcc aaaatccacc ggtttaaaac aggccagctc
     2461 caatatcaga atgcgatgga tcaagctcaa gaattcatgc gcatttacgg atcatcgctc
     2521 ccgattgagc acaaaaggac attagagcat ttattagaaa acaggaaacg gttttggttg
     2581 gatcggctac ggtatgcttt atcatgtgag gtataccatc aatcgacttt tgaacacttt
     2641 atcttgaagg cgttaattgc attaaatcgc ctgtaattgc aggttgaaac tttttactaa
     2701 gtcccatgat tccagaggta tacaatccaa tgttactcag ctttgatcat tattgcgatc
     2761 aaaaatttag agcaaccagc ttgcattatt atctgagcct tcatcaacaa atattgattt
     2821 cgaatgagca aaaactacaa ttccttatat aatcttgctt gccttaatta tgaaggagct
     2881 cttgaatttt cctgatccat acgacaaaaa cctttccgcc gttgcctttt gcacatgaag
     2941 cagaaaccct taaccccggt accctttcgg cacatgaaat gtaagcatga ttgttaaatc
     3001 agaaacagaa tgatataaat tctatcttag gtcattcagt ttcttctagc accaccgatt
     3061 acgagatgcc aagtaactcg aactatattt ccataatcgt tccagtgtat aacaaccctc
     3121 aggatctact cgagtgcctt tccgctctta gggcctcata ctatcctaat tcggagatta
     3181 tcgtcgtaga cgatgcctcc acggatgaca cccatttaat cgccacgagg acaggggtgc
     3241 gcgtcctacg gcttacggag aactcagggc ctgcaccagc gaggaaccat ggagcccgtc
     3301 atgcgaaggg caatatccta atctttgttg atgctgacgt cgtcgtttct ccaggggtga
     3361 taagccgaat ggtaaaggtg tttgaggagc atccggatgt ggctgccgtc tttggctcct
     3421 atgatgcctc tccccgtgcc gatggtgtgg tgtcccagta tcggaacctg ctccatcact
     3481 ttgtccatca gaatggcaac cctgaggcct cgacattctg ggcaggattc ggcgcaatcc
     3541 gccgctcagt attcgaggag attggcggct tcgatgagaa gcgcttcccg aaatgctcca
     3601 tcgaggatat tgcccttgga tactatctac gaaaagctgg ctatagaatc ctcttggata
     3661 aatccatcca ggtgacgcat ctgaagcgat ggaccctccg ttctgtcatc actgccgata
     3721 tcttctatcg cgccgtcccc tggtcgaagt taattcttga tgagggtaat ctgccacgtg
     3781 atttgaactt acaactatct cagagggcaa gctcagtgtt agtctcctta ttgatcatta
     3841 tgttaactct tgcct
//
LOCUS       DATDRL010000295         7919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1917660, whole genome shotgun sequence.
ACCESSION   DATDRL010000295 DATDRL010000000
VERSION     DATDRL010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917660"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VI935_09945"
     CDS             <1..802
                     /locus_tag="VI935_09945"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HLE25955.1"
                     /translation="HGYKLSDKSSLEERLKEFQRRNKVPATGRLNSQTCRLINSYRCV
                     HPEKAIPFEEEQSRFQATDYLPLKLAQDRAYLVDTEYNIDTTLLLKGEHTHELINGRW
                     SRRTLGFALVGSSPSYLGSHAWEAVRQSFKAWGKTGIIRFEERKDPARAEIRVLWTPG
                     PNVDPNSTDPFYGPGEKIAIGYYPYPHLNELAGDLHLDISENWVIHPSHKGFDVETVA
                     LHEIGHCLGIGHSTIQDSIMWSIYKSNQRHLIQEDKQELERIYQAIPL"
     gene            complement(1366..2463)
                     /locus_tag="VI935_09950"
     CDS             complement(1366..2463)
                     /locus_tag="VI935_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HLE25956.1"
                     /translation="MRTGFAHLPLHSGKAPRWLFNNMVCLAREIVSHITTEYGASDVL
                     IRLSDPFWFQALGCVLGFDWHSSGVTTTTCGAIKEGIRGLEKELGLFAAGGKGASSRR
                     TPVEIMNACDHISQEPENLIKASRLSAKVDNSAVQDGYQLYHHSFFFTQDREWCVVQQ
                     GFNDSNSMARRYHWLGSRLNSFVNEPHSAICCDANGNTLDLVASDSEQTRNAITELAS
                     QPDRGVLRDIERLPNLVLPHRHQVTAADVNPKYLKKVLLSTYELAPKNFEELLGIHGV
                     GAKTLRALTLVAELIYGTRASTRDPARFSFAHGGKDGTPFPVDRRTYEQTITVLHDAL
                     AKLRVDYSEKIRALKRLAAFDQNNQNYKLIT"
     gene            complement(2522..3586)
                     /locus_tag="VI935_09955"
     CDS             complement(2522..3586)
                     /locus_tag="VI935_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003963629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HLE25957.1"
                     /translation="MSIPTEPIGSIPRPGGLIKGIQAFKDGRISQKELNSLYDSAIRN
                     TIELFEATGSPVITDGEQTKPSFATYPIHGLENIAPDGVMIHFADGHVRQLPRLNAGP
                     FHYKTYADAYLEVAKKYAHVPVKQAVISASALSLLYPQDGIGGYSREAFIEDLLRENG
                     ADIRRCLQKGAFKVQIDFTEGRLAVKLDPSGQLLESFIDLNNRVLSRFSVGERNNIGV
                     HTCPGGDQDSTHSADVDYAKLLPSLFKLKVGNFYIQLASESDRTRVLKIIKENAKPDQ
                     RIFIGVIDPINPRVETPEEVRDRVLEAAEYISLSQLGTTDDCGFSPFGDDTSTTRETA
                     FEKIRARVVGTSLAAKKLGV"
     gene            complement(3766..6474)
                     /locus_tag="VI935_09960"
     CDS             complement(3766..6474)
                     /locus_tag="VI935_09960"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HLE25958.1"
                     /translation="MEEANKIELDKTYDPKKVEEKWLGFWIKKGYFQASTSSQKPSFT
                     IVIPPPNITGSLHIGHALNNTLQDILVRYKRMKGFNGLWVPGTDHAGIATQNVVEREL
                     LKEGIKREDLGREGFIKRVLKWKELYGNRIIVQLKRLGASCDWSRERFTMDEGLSIAV
                     KQAFVRLWRDGLIYRGHYLVNWCPRCDTALSDLEVTREDTKGKLYYLRYPLASPTGDK
                     GTKDYIVVATTRPETMMGDTAVAVNPKDERFKHLIGKEVIPPVVNRKIPVIADESVDM
                     EMGTGALKITPAHDFADFEIGTRHDLPRVQIMDFEGVMNENAGRYSGLDRFECRKRIV
                     EDFEKEGVLEKVEDYQLQLGKCYRCGTIVEPMLSLQWFVRTKPLAFPAIQSVEDGRTR
                     IIPEGWKNTYFEWMRNIRDWCISRQIWWGHKIPAWFCKDCEGITVSVDEPRVCEHCAS
                     RHIEQETDVLDTWFSSALWPFGTLGWPRKTKELEIFYPTSVLVTSFDILFFWVARMMM
                     MGLKFMNDVPFRDVYIHALVRDEKGEKMSKTKGNVIDPLDIMDEFGADALRFSLTALA
                     AQGRDIKLSLPIIEGYRNFMNKIWNASRFVFMNLNDAGARHASPLGSNLSGPDKWILT
                     KLNRTMQEIEISLEVYEFDKTARLIYQFIWGDFCDWYIELVKPILNGDDLEARKRTQA
                     ILVRILSSSLQLLHPIAPFITEEIYQRLRELGVRMPTADEEEAESISISSYPNFNKDE
                     IYEEAFDEIEYIKEVIVGIRNLRAIVGVHPSKMVDIILLTDNEKILEITHANKNFIRS
                     LAKASFDLSQKEKPKKAVAQVIEGLQIFLPVEGLVDVEKEVERIKREITKVVKDVELT
                     NRKLSNPDFLERAPEDVIEKEKEKFEELSLKKRGMEEVLQKLSEIG"
     gene            complement(6556..>7919)
                     /locus_tag="VI935_09965"
     CDS             complement(6556..>7919)
                     /locus_tag="VI935_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HLE25959.1"
                     /translation="AAQSIGEPGTQLTMRTFHIGGAASQRVAESTLESRHEGVVKLIG
                     VRAVRNKEGRLVVINRTGILAIVDEMGYERERYPLVLGAKLRAEESDQIKVGSLLAEW
                     DPYTTPFISEVDGRVSFQDLEEGISMKEQVDEVTGIFKKVVVESKNPDLHPAIVVTDK
                     HGKAAQYLLPVGAIIERNEGDMVHGGDVFAKIPRETAKTKDITGGLPRVAELFEARLP
                     RDPAIVSEIDGIVSFGKDSKGKRKMVVTPDIGEAKDYLVPRGKHVLVRDGERVKAGDQ
                     LVDGAVNPHDVLRIRGEKALARYLVDEIQQVYRLQGVKINDKHIEIIVRQMLKRVRVK
                     DPGDTTFLVGEPVERIVFFEENERVTSQGGKPATAEPLLLGITRASLSTDSWLSAASF
                     QETTRVLTEAACEGKVDSLRGLKENVIMGRLIPAGTGLPMYRNVEVEIKVPEIPVPTP
                     VKE"
BASE COUNT         2107 a         1954 c         1539 g         2319 t
ORIGIN      
        1 tcatggttac aaattaagtg ataaatcgag cttagaagaa aggctcaaag aattccagcg
       61 gagaaataaa gtacctgcta ctggacgtct aaactcccaa acatgcagat taataaattc
      121 ttacaggtgt gtgcatcctg agaaagcaat cccctttgaa gaagaacaga gccgatttca
      181 agcgacagac tatctaccat tgaaattagc tcaggacaga gcttatctag ttgatacgga
      241 atacaacatt gacactacac tgttgttaaa aggcgagcac actcatgaat taataaatgg
      301 aagatggtca cgacgaacat taggatttgc gctcgtcgga tcttcaccaa gttatttggg
      361 ttcacatgca tgggaggctg ttaggcaatc attcaaagca tggggtaaaa cagggataat
      421 aagatttgaa gaaagaaaag atccagcaag agcagagata agggtgttat ggacaccggg
      481 accgaatgtg gatccgaatt ctacagatcc tttttacgga ccgggagaaa agattgcaat
      541 tggttattat ccatacccac atctgaatga acttgcggga gacctgcatc tcgatattag
      601 cgaaaactgg gttatacacc ctagccacaa aggatttgat gtagagactg ttgcattgca
      661 tgaaattggg cattgtcttg gaataggtca ttctacaatt caagactcta tcatgtggag
      721 tatatacaaa tctaatcaaa gacatctcat acaagaggat aagcaagaac ttgaaagaat
      781 ttatcaagcc ataccgttat aaaagttttt cctcttgtat aaagtaaaac taggctcagc
      841 tcttgaatgg tccagctagt tgggttgggt tcgaatattc aaatattgct tcccgaaaat
      901 tagagggcac tgtcttgctt tttgaattta tgtcaagttt ctaactcatc ttactcatat
      961 tctacagctt gtcatgcccg aatgttcccc gctaaagctt gcactcgaat gttttaatcg
     1021 agcgacatac cgggacaagc tttatcggtc atcgccaccg ttgtcatacc cgaatctttc
     1081 tatcgggtat ccatgtttta gattcccgct taaacttgta cccacacatc taaagtgggt
     1141 aacatgcggg aatgacagct ccttacctcg attaggtgta tgagtaggca acatctgttt
     1201 ctaatttata agctaatcct cgatgtctct aatacattca aattagccgt aagctcccct
     1261 tcagtaaata atgatttgtt aatttccctg cgaaatatgc ttcgtaggcg cttatgtcaa
     1321 aatgaccgta accacctagt ttgaataata tagtttgtag aatcattatg taatgagttt
     1381 gtagttttgg ttattttggt cgaaagccgc taggcgcttt agggccctta ttttctcact
     1441 gtaatccacc cttaatttgg ctagagcatc atgaagaacc gtaatggttt gttcgtaagt
     1501 tctacgatcg acggggaaag gagtaccgtc ctttccacca tgtgcaaatg aaaatcgcgc
     1561 gggatctcgg gtgctagccc tggtgccata gattagctcc gctaccaatg ttaatgccct
     1621 aagtgtcttt gcacctactc catgaattcc caataattcc tcaaagttct taggagcaag
     1681 ctcgtatgta ctcagtaaga ctttcttaag gtactttgga ttaacgtcgg cagcagtaac
     1741 ctgatggcgg tgcgggagga ctaggtttgg gagtcgctct atatccctca atacacccct
     1801 gtcaggttga ctggcaagct ctgtaatggc atttcgagtc tgctcactgt ctgaagctac
     1861 taggtcaagt gtattaccgt ttgcatcaca gcaaattgct gaatgtggct cattaacaaa
     1921 actgttaagt cttgaaccaa gccagtgata acggcgtgcc atggaattcg agtcattgaa
     1981 tccctgttgc actacacacc actccctatc ctgcgtgaag aaaaagctgt gatgataaag
     2041 ctgatagcca tcttggaccg ctgagttatc aaccttagcc gacaggcgac tcgccttgat
     2101 gagattctct ggctcctgtg agatatgatc acaggcattc ataatttcaa ctggcgttct
     2161 tcttgaagaa gccccttttc ccccagcagc aaataatcca agctcctttt ctaaacctcg
     2221 tatcccctcc tttattgcgc cacaggtcgt ggtagtcacg ccgcttgagt gccaatcgaa
     2281 tccgagaacg caaccgaggg cttggaacca gaatgggtct gacagtctga taagaacgtc
     2341 tgaagctccg tattctgtag ttatatgaga aacaatttct cgagcaaggc acaccatatt
     2401 gttaaaaagc cagcgtggcg ccttcccgct gtgaagtggg aggtgagcaa atccggtacg
     2461 catgatgaag atattagctt ggcaggtatt ttaagtcaat cgaatagaac cactagccct
     2521 atcaaacccc tagctttttc gcagccagcg aagtgccaac cacacgggca cggatttttt
     2581 cgaaagccgt ttctcgggtc gttgaagtat catcaccgaa tggagaaaaa ccgcagtcgt
     2641 ccgtcgttcc cagttgacta agcgaaatgt attcagcagc ttcaagtacc cgatccctta
     2701 cttcttccgg cgtttccact ctgggattta tagggtcaat cacgccgatg aaaattctct
     2761 gatcaggttt tgcgttttcc ttaatgatct tgagaactct agtacggtcg ctctcgctgg
     2821 ctaactggat gtaaaaattc ccaaccttaa gtttgaacaa gcttggcaag agcttggcat
     2881 agtccacatc cgcactatgg gtcgagtctt ggtctccacc cggacaggtg tgaacaccga
     2941 tattatttcg ctcccctacc gagaaacgac ttagcactcg attgttaaga tcgataaaac
     3001 tttccagaag ttgcccgctt ggatctagtt tcacagcgag tcttccttca gtaaagtcaa
     3061 tctgaacctt aaaagcgccc ttctgtaggc agcggcgaat atcggctccg ttctcacgga
     3121 gaaggtcttc gataaaggcc tctcttgaat agcccccgat cccatcttgt gggtagagaa
     3181 gactcaatgc cgatgcagag atgaccgctt gctttacagg aacatgagca tatttttttg
     3241 ctacttccaa atatgcatca gcataggtct tgtaatggaa tggtcctgca ttcaacctag
     3301 gcaactggcg cacgtgaccg tcagcaaagt gaatcatgac accatcagga gcgatgttct
     3361 caagtccatg gattggatat gttgcaaagc tcggcttggt ttgttccccg tccgtgatga
     3421 ccggcgaccc tgtcgcttcg aagagctcta tggtgtttcg gattgctgag tcatatagtg
     3481 agttcagttc cttttgtgaa atacgaccat ctttgaacgc ctgtatcccc ttgataagcc
     3541 cccccggtct tggaatactg ccaataggtt cagtaggtat tgacatatta gcctcctaaa
     3601 caaaattaag gttattcaat attttacttt actggactcc cagcacgcaa ctgcatcgct
     3661 gctaagcgct acccaagaga ggatatggcg aaaatggtcc cctaaaatcc tccctctttg
     3721 gtcactacct cagatcccga tgtcacctaa aatctttatc gagatttaac caatctcact
     3781 tagtttctga agaacctctt ccatcccccg ctttttaaga gaaagctctt cgaatttctc
     3841 tttctccttt tcaattacat cttctggggc acgctcaaga aaatcggggt tagaaagttt
     3901 tctattggtt agttctacat cttttactac ctttgtgatt tctcgcttta ttctctcaac
     3961 ctctttctca acatcaacta acccttccac tggaaggaaa atctgtagac cctcgattac
     4021 ctgtgcaacg gctttcttag gtttttcttt ttgagaaagg tcaaacgatg cctttgctaa
     4081 gctacgaata aagtttttat tagcatgagt tatttcaagt attttttcat tatcagtaag
     4141 aagaataatg tctaccattt ttgaaggatg tacaccgact atagctctta ggtttcttat
     4201 tccgacaatg acctctttta tgtactcgat ttcgtcaaac gcctcttcgt aaatctcatc
     4261 tttgttgaaa ttaggatagg agctaatgga tatgctttcc gcctcctctt catctgcagt
     4321 tggcattctg accccaagct cgcgaagcct ttgatagatc tcctcagtta taaaaggagc
     4381 aattggatga agaagttgta gggaagagct aagtattctt accaaaatcg cttgtgttct
     4441 ctttcttgct tcgagatcat cgccatttaa gattggtttt acaagctcta tataccaatc
     4501 acaaaaatca ccccagatga attgatagat aagtcgtgcc gttttatcaa actcgtatac
     4561 ttctaaagaa atttcaatct cctgcatggt tctattaagc tttgtgagaa tccacttgtc
     4621 aggaccagag agattagacc caaggggcga ggcatgcctc gcccctgcat catttaaatt
     4681 cataaacaca aacctcgatg cattccagat tttattcata aagtttctgt acccctcgat
     4741 tataggaaga gaaagcttta tatcccttcc ctgagcggca agtgcggtga ggctgaatct
     4801 taaagcatcc gctccaaatt catccatgat atcaagtgga tcaatgacat tcccctttgt
     4861 tttactcatc ttctcaccct tctcatccct gacaagggca tggatataaa catctctaaa
     4921 gggcacgtca ttcataaact taagccccat catcatcatc cttgcgaccc agaaaaagag
     4981 tatgtcaaag cttgtgacta gaacggaggt aggataaaag atctcaagct ctttggtttt
     5041 tctaggccat cctagcgttc caaatggcca tagagcggag ctgaaccatg tatcaaggac
     5101 atcagtttct tgctcgatat ggcgagatgc gcaatgctca caaaccctgg gttcatcaac
     5161 cgaaactgtt atcccttcac aatctttaca gaaccaagct ggaatcttat gcccccacca
     5221 tatttgtctt gatatgcacc agtcccttat attcctcatc cattcaaaat atgtgttttt
     5281 ccaaccctca gggataatcc ttgttctacc atcctctact gactgaatcg caggaaacgc
     5341 aaggggttta gttctcacaa accactgaag agaaagcatc ggctccacta tagtcccaca
     5401 cctgtaacac tttcctagct ggagttgata atcttcaacc ttttcaagta caccttcctt
     5461 ctcaaagtct tctacgattc tctttctgca ctcaaaccta tcaagcccac tgtatcttcc
     5521 agcgttttca ttcatcactc cctcaaaatc cataatctga acccttggaa ggtcgtgtct
     5581 tgtgccaatc tcaaaatcag caaaatcatg cgcaggtgta atcttcaaag cccctgtacc
     5641 catctccata tctacgcttt catcagcgat aacagggatt tttcggttta caacaggagg
     5701 gattacctct ttaccgatta gatgtttaaa tctctcatct ttaggattaa ctgcaacggc
     5761 ggtatcaccc atcatcgtct caggtcttgt cgttgcaaca actatgtaat ctttggttcc
     5821 cttatcacca gttggagaag caagtggata cctgagataa tatagctttc cttttgtgtc
     5881 ttcacgcgtt acctccaggt ctgaaagggc tgtatcgcaa cgcggacacc agtttacaag
     5941 ataatgccct ctgtaaataa gcccgtctct ccaaagccta acaaaagcct gtttaacagc
     6001 aatagagagc ccctcatcca tcgtaaatct ctctcttgac cagtcacaag aggcaccaag
     6061 cctttttaac tggacaataa ttctatttcc atatagttct ttccacttta aaaccctttt
     6121 tataaatcct tctctaccca gatcctcacg cttgatcccc tctttaagga gctccctttc
     6181 aacaacattc tgggtagcaa ttcccgcatg gtcagttccg ggaacccaga ggccgttaaa
     6241 ccccttcatt ctcttatatc gaactaatat atcctggagc gtgttattta aagcatgccc
     6301 tatgtggaga gaaccagtta tattgggagg tgggataaca attgtgaagg agggtttttg
     6361 agacgaagtt gaggcttgaa aatagccttt tttaatccag aacccaagcc acttctcctc
     6421 tacctttttg gggtcatagg ttttgtctaa ctctatctta ttcgcctctt ccatgttcgt
     6481 agttcgtgaa tcgggtatcc tatcattgga atgagcccac gacgaagtca caaatcacga
     6541 accccgggct acgacttact ctttaacagg ggttggaacg ggtatttccg gcactttgat
     6601 ttcaacctct acgttcctat acataggcag cccggtgcca gcaggtatca atcttcccat
     6661 aatcacattt tcttttagtc cgcgaagact atctaccttc ccttcacaag ccgcctctgt
     6721 aagtaccctt gtagtttcct ggaatgaggc cgcagaaagc cagctatcag tgctgagaga
     6781 ggctctagtg atacctagaa gaagtggctc tgctgtggcc ggttttccgc cctgagaggt
     6841 aaccctttca ttctcttcaa agaagacaat cctttcaact ggctctccaa caaggaatgt
     6901 tgtatcaccc ggatccttaa ctctcacgcg ctttaacatc tgccttacta taatctctat
     6961 gtgtttatca ttgatcttaa ctccctgaag cctgtagacc tgttgtattt cgtctactag
     7021 atatctcgca agggccttct caccacgaat tctcagtaca tcatgaggat ttacagcgcc
     7081 gtcaacgagc tggtctccag ccttcacacg ttcaccatct ctgactagca catgctttcc
     7141 tctcggtacg aggtagtcct tcgcttcacc aatatctgga gttacaacca tctttctttt
     7201 accctttgaa tcctttccaa aagatacgat tccatcaatc tcactcacaa tcgcggggtc
     7261 tctcggaagc cttgcttcaa agagctctgc cacccttggc agtcctccag tgatgtcctt
     7321 ggtcttagcc gtttctcttg gaatctttgc aaatacatcc cctccatgaa ccatatcacc
     7381 ttcgttcctt tcaataatcg ctccaactgg aaggagatac tgggctgcct ttccatgttt
     7441 atcggtaaca actatggcag gatgtaaatc tggatttttc gattcaacaa ccaccttttt
     7501 aaaaattcct gttacctcgt ccacctgctc cttcatgctt atgccctctt caaggtcctg
     7561 gaagcttaca cgcccatcca cctctgagat aaatggagtg gtgtatggat cccactcagc
     7621 aagaagcgaa cccactttta tttggtcact ctcttcagcc ctcaacttcg ctccgagaac
     7681 gagtggatac ctttcacgct cgtatcccat ctcatccact atagctagaa ttccggtacg
     7741 atttattaca acgagtcttc cctctttatt cctcactgcc ctaacaccga taagctttac
     7801 aacaccctcg tgtctgctct caagtgtact ttctgcaacc ctctgactag cagcaccacc
     7861 tatatggaag gttctcatag taagctgggt gcccggctcc ccaatagact gggctgcta
//
LOCUS       DATDRL010000296         3602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1951858, whole genome shotgun sequence.
ACCESSION   DATDRL010000296 DATDRL010000000
VERSION     DATDRL010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951858"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..936)
                     /locus_tag="VI935_09970"
     CDS             complement(46..936)
                     /locus_tag="VI935_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25960.1"
                     /translation="MGGFKLLQIVVYSLSIIAFFIGLDYFSSKPSIEIVNFFTIPNDA
                     SEIAINSLLPFYKKNDLNIPQYFQTMPRLKNLFFYEKEEDSGIGFTPPEVLEAIKKYK
                     DFDVPNDLIKELHESVQKFKYKKGSWVFGGGRIIKKENLNKILKLLKENLTKNEHYTF
                     LAAFIYSRVFYNQIILKNDGDIDLKDIKLLIPSPFSRVSNTRDNNILDFEPHSVIFYD
                     IEKLENGLILQIPYLKKGDFFSFNIVSRESQIDVEEIMSNFKEDFKIYNKRKIFVGVP
                     IVFIIMVIICICLKGKNIKD"
     gene            complement(1318..2313)
                     /locus_tag="VI935_09975"
     CDS             complement(1318..2313)
                     /locus_tag="VI935_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HLE25961.1"
                     /translation="MSNEIWVVADLTIDGKVRKVTFEGLSEARRKLAGKLGGQLCGVL
                     LGSGVSGLASELGKYGAEKVYVVDNELLKDYTPEGYSIALSELIKKDEPHIVITGNTS
                     VGQDYFPRAAARVGAGLTLDAIDLDLTDDKRLKVRRYMYSAKSIATVEFLDHKPMMTT
                     IRPNVFKPEEASKNPEVINESIDIKPEQILTKTVEVKRKESTRPELTEADRIVSGGRG
                     MASGDNFYLIFNLADAIGAAAGASRAAVDSGFVPYEMQVGQTGKVVSPTLYIAIAISG
                     SVQHYAGMGSSKVIVAINKDQDAPIFQKCDYGIVADLFQLVPALTEEFKKLLAQQ"
     gene            complement(2416..3231)
                     /locus_tag="VI935_09980"
     CDS             complement(2416..3231)
                     /locus_tag="VI935_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002748872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HLE25962.1"
                     /translation="MKVVVIVRQTPDTEAKIKVGSGGNSIDIEGIKWIVSPYDEFAIE
                     EAIKIKEKLGTGEVILITMGPARCVEALRTGLAMGADSAIHIKDESFSFTDPYAIAKV
                     IGQEIRSLGGFDLILTGKKMIDEETGQVGIHVAEDLGIPHIAIVTKVELSSDKKKVTC
                     QKEIEGGQVVAEVPLPALLTCERGLNEPRYASLPGIMKAKKKPVKEVTPDNINVETLG
                     LTKEALGQAGARFKVTKLEVPQVQRKLKVIKGAEVPEAAKELVKLLREEAKVI"
BASE COUNT         1051 a          758 c          600 g         1193 t
ORIGIN      
        1 gttatactac gtagacaaaa tggttaagaa gtgagaggaa ctactctagt cttttatatt
       61 tttccctttt aagcaaatgc aaataatcac cataataata aatactattg gaacaccaac
      121 aaatattttc cttttgttat agattttaaa atcctctttg aaattggaca ttatttcttc
      181 tacatcaatc tgactttctc ttgatacgat attaaaagag aagaagtctc cttttttaag
      241 ataggggatt tgcaaaatca aaccattttc tagtttttca atgtcataaa aaataacaga
      301 atgcggttcg aaatctaata tgttattgtc tctagtattt gaaaccctac taaatggtga
      361 tggaatcaat agttttatat cttttaaatc aatatcacca tcatttttta atataatttg
      421 attgtaaaat actcttgaat agataaaggc agctaaaaac gtgtaatgct catttttagt
      481 tagattttcc tttaatagtt tcaatatttt atttaaattc tcttttttta taatacgtcc
      541 tcctccaaaa acccaagatc cttttttata tttaaacttc tgaacagact catgtaactc
      601 ttttattagg tcattaggaa cgtcgaaatc cttatatttt tttatagcct ctagcacttc
      661 cggaggggta aatcctatgc ccgaatcttc ttctttctca taaaagaaca gattttttaa
      721 tcgaggcata gtttgaaaat actgaggtat gttaagatcg ttttttttat aaaagggcaa
      781 taaagaattt atagcaattt cactagcgtc attagggata gtaaaaaaat taacaatttc
      841 gatagagggt tttgaggaaa aatagtctaa ccctatgaaa aatgctataa tagataagga
      901 ataaacgaca atttgcagaa gtttaaatcc acccaacgat gaaacttctt gaaaaaacct
      961 tttaattaac cttattagtg agagcattaa ttgtacagtc aataatacta tagatgatcc
     1021 atatcttcta attcggttta aaagttttat aaaagctcca acaaaaaagt tacaatcggg
     1081 aatcactgtt tttttcttat tataaacaag aatctttcat aaaaaaattt aatagatacg
     1141 taggagtata tagaagataa tctaggtaaa aagaccttgc acaacttaaa atgtaagcga
     1201 agtggtataa gtttctgttc ttacaaagct gcaagttcga atgttagttc aggacgaagt
     1261 ccgaaatttg caatcgcgga tcggtattaa tgcttatacc cacggagatt ggaaaactta
     1321 ctgctgcgca aggagttttt taaactcctc cgtaagcgca gggaccaatt ggaatagatc
     1381 agccacgata ccgtaatcac atttctgaaa tatcggcgca tcctgatcct tattgattgc
     1441 aacaatcacc ttagaagatc ccatccccgc ataatgctga accgagccag atattgcgat
     1501 tgcaatgtaa agagttgggg atacgacttt cccagtttgt ccaacctgca tttcataagg
     1561 aacaaaccca gagtctactg ctgcacgtga ggcaccagcg gcggcaccaa tcgcatctgc
     1621 aagattgaaa atcaggtaaa aattatcccc gctcgccata ccgcgcccgc ctgaaacgat
     1681 tctatccgcc tctgttagct ctggtcgtgt agattccttc ctcttgacct caacggtctt
     1741 tgttaagatt tgttcaggtt taatatcaat ggactcgttt ataacctctg gatttttaga
     1801 cgcctcttct ggtttaaata cgtttggtct tattgttgtc atcatgggtt tatggtctag
     1861 aaattcaact gtggcaatgg attttgcaga atacatatag cgtctaactt taagcctttt
     1921 atcatcagta agatctaaat caatagcatc aagagtgagc ccagctccta cgcgagcagc
     1981 tgctctaggg aaatagtcct ggccgacaga ggtgtttcct gtaattacaa tatggggttc
     2041 atctttttta atcaattccg agagcgcaat agagtatcct tcaggggtgt aatcctttaa
     2101 gagttcgttg tctacgacgt aaaccttttc tgccccgtat ttccctaact ccgatgcaag
     2161 acctgacaca ccagagccaa gaaggactcc gcataactgt cctccgagtt ttcctgcgag
     2221 ttttctcctt gcctcagata aaccttcaaa ggttactttc ctaaccttac cgtctattgt
     2281 aagatctgct actacccaaa tttcgttact catttgtttc ccccttaaat tagttagccg
     2341 atagatatta gccctcagca atcagtaaaa gctttaggtt gatggctgat taatgatagc
     2401 cgattgcttt tcattttaga taaccttcgc ctcttctcta agaagcttta caagctcttt
     2461 tgcagcttca ggaacctcag ctcccttaat cacttttagt tttctctgta cctggggaac
     2521 ctcgagtttc gtaaccttaa atcgcgcacc cgcctgacca agagcttcct tggtaagacc
     2581 caatgtttca acattgatat tatcaggggt aacttccttg acaggttttt tcttggcctt
     2641 catgattccg ggtagggaag cgtatctagg ctcatttaac cccctttcgc aggttagaag
     2701 cgctgggaga ggaacctcag cgacaacctg tccaccctcg atttcctttt ggcaggttac
     2761 ctttttcttg tccgaagata attcaacctt agtgactatt gcaatatgtg gaatacctaa
     2821 atcctcagcc acatgaattc ccacctgtcc tgtttcttcg tcaatcatct tcttacctgt
     2881 aagaatcaga tcgaatccac ctaggctcct gatttcctgc cctatcacct ttgcaattgc
     2941 ataaggatct gtgaaagaaa aactctcatc ctttatgtgt attgcgctat cagcgcccat
     3001 tgcaagccct gtgcgcaatg cttccacaca tcttgcaggc cccattgtga tgagaataac
     3061 ctcaccagta ccaagctttt ctttaatttt gattgcctct tcaattgcaa attcatcata
     3121 agggcttaca atccatttta tcccttctat atcaattgaa ttcccaccgg acccgacttt
     3181 aatctttgcc tccgtatccg gcgtttgtct tacaattaca acgactttca ctttgctttt
     3241 cctccttaat tttgttttag ggctggttta taacttttct ggcaattacc aatcgttgga
     3301 tttgattggc accttaataa atttaaatta gtttagcatc cctctctagc ttttcaatta
     3361 gataatattt tgcgtacctg tagactccaa atatgtattt gacaccgtta gtaaacagta
     3421 tcaaaaacat aggcccgcta aattgtttaa cttgagaatt tcctgtagct tgctacaggg
     3481 tttccacaat agtgtattgt cattgcgaac gaagtgaagc aatctcttat tttagattgc
     3541 ttcgtcgctt ttctcctcgc aatgacagct ataacggtat atttttaaga ccataccctg
     3601 ca
//
LOCUS       DATDRL010000297         5057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2181198, whole genome shotgun sequence.
ACCESSION   DATDRL010000297 DATDRL010000000
VERSION     DATDRL010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181198"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VI935_09985"
     CDS             complement(<1..241)
                     /locus_tag="VI935_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HLE25963.1"
                     /translation="MRRVDALVEMRQQEINRLDVSDAVIKKTIGDHINYLTKQIDSIK
                     EQINKHIDRHPQLRDKQKLLQSIPGVGEATIHLVLA"
     gene            667..1353
                     /locus_tag="VI935_09990"
     CDS             667..1353
                     /locus_tag="VI935_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011887281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HLE25964.1"
                     /translation="MTLTTDKDDLQSRFGYVDPSGVLFKPRYNIAPSQDSPVVTVKDD
                     NRVLIMMRWGLVPFWAKEASIGYKMINAKSETIAEKPSFRKPFKEKRCLVLADGFYEW
                     TKTDKKNKVPFRFVLKTKKPFAFAGLWDAWKSPEGEILLSFTIITTTANELMEPIHDR
                     MPVILHEKDESLWLDPEFKDTNKLAALLKPFPSDMMEAYRVSTIVNSPKNDSPDCIRP
                     VLSQVEGPVK"
     gene            1395..1823
                     /gene="umuD"
                     /locus_tag="VI935_09995"
     CDS             1395..1823
                     /gene="umuD"
                     /locus_tag="VI935_09995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HLE25965.1"
                     /translation="MDVIEMYRLKRGVKLNLPLLLSRLPAGFPSPADDYIEERIDLNK
                     KLIKHPEATYLIRVMGDSMEKARIYDGDYLIVDRALEPEHNDIVLAIFDGEFTVKRLI
                     KKIDRWFLVAENPKYPPFEVTPETDFEIWGKVTYIFHPAV"
     gene            1830..3146
                     /locus_tag="VI935_10000"
     CDS             1830..3146
                     /locus_tag="VI935_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HLE25966.1"
                     /translation="MQKIIGLIDCNNFYVSCERVFNPRLRGRPVVVLSNNDGCVVARS
                     EEAKALGIPMGAPAFQHQALFNQHKVETLSSNYTLYSDMSQRVMSILSRFTPELELYS
                     IDEAFLDLSGFTNFNLTEYAKEIKATVYKWTGIPISIGIGPTKTLAKLANKLAKKNKM
                     CGGVFDITDHPRIDDFLSSVKVEDVWGVGRQYTKLLNRNWISTALDLKNAPDIWIKKN
                     MSIVGLRTVWELRGISCIELEDLSEPNKQIIRSRSFGKPVQTLQELKEAVTFHATRAA
                     EKLREQKSVTFFISAFIETNRFRTEEPQYSNSAGCYLPEPTAYTPLLIKNALACLERI
                     YREGFKFIRAGVVLMDIVPEDQIQLNLFVPSRPLEKDKSMMKAVDRINVKWGSNSIKY
                     ASSGLEQPWRMKRAKLSPRYTTHWDEMLVVKCSPPCYLSRVATSHQ"
     gene            3443..3880
                     /locus_tag="VI935_10005"
     CDS             3443..3880
                     /locus_tag="VI935_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLE25967.1"
                     /translation="MAGRKFDPNRLTFEQGIERIQKAIKKSGVEIEGRIVTSRDEGMF
                     KLRELLRTDNKPNGFQKYQLPVALRCESQMFLTVAEPGAKAPRHAHKEGDGIRFIVAG
                     SIIYDGKELTSNDWMFIPAGAPYSFEVGPHGATMFYCYCCCCA"
     gene            4150..>5057
                     /locus_tag="VI935_10010"
     CDS             4150..>5057
                     /locus_tag="VI935_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25968.1"
                     /translation="MATKKTSSQKNREAKTFKLDIAVHRRILNFLNEAIQPEDLVYEK
                     VPLPNPEVDPIHEDNPEELKLKRKKILDFKVAKEVIEFRDQEYPLGFRNLKEVLDLKL
                     FDRRHLDVLLHHFSNMFYGSWSVFPQSIPRRGPGGYDGVVHAALLHTGKVLFITADET
                     TLLWNPDDTTPATFEDPVTQPHLTPDAMSGYSVLCGGHSFLSDGRLLVVGGGGYGPHS
                     KAKWGYKFDPTSRTWTRTANSMVHDRWYPTVMTLGDQRIANSHEILVVCGHGSGDMEI
                     YDEASDSFVEVTSGDDKPFPNLYPGL"
BASE COUNT         1502 a         1094 c         1143 g         1318 t
ORIGIN      
        1 tggctaacac taaatgtatc gttgcctcgc ctactcctgg gatgctctga agtaatttct
       61 gcttatctct taactgtgga tgtctatcaa tatgtttatt gatttgctcc tttatagaat
      121 ctatttgttt tgtgagataa tttatatgat ccccgatagt cttctttatc actgcatcac
      181 tcacatctaa acgattaatc tcttgttgtc gcatctcaac caaagcatca actcgtctca
      241 caagcgagcg taactccctg atttccttag attccggtgt cggaataaag gggacgcaac
      301 ccttattttc tcagaaacat aatcaactga tattttttga ttgatttcca gccggatata
      361 ataccaattc tctattgaaa tgcaccatta acggttctca caattctata aagaaatatg
      421 ttgaaaaaga ttgcttcgtc gcggagttta tcctgaaggg aataatgagc aagagggtca
      481 ggcttttgtt gttttgttat tgtattcaac tatctatccc aagaaaaagc cagaaaaagg
      541 ggaacaacag accccgtatt tctcaaagaa acttccaaaa gccctttact gaagaactat
      601 ctttttccca tctctttgct tcacctatcc cttcaatgat aaacttactt cattatgtgc
      661 gggcggatga cccttaccac cgacaaagac gacctgcaaa gcaggttcgg ctacgttgac
      721 ccgagcggtg tcttgtttaa accacgatat aacatagctc cttctcagga ttcgcctgtg
      781 gttacagtca aagatgacaa tagagtgctt atcatgatgc ggtggggtct tgttcctttc
      841 tgggcgaagg aagcttctat tggttacaag atgataaacg caaaatcaga aaccatagct
      901 gagaagccga gctttagaaa accgtttaaa gagaaaaggt gtctcgttct tgctgacggg
      961 ttctacgagt ggacaaaaac agacaagaaa aacaaagtac cttttcgatt tgtactcaag
     1021 acaaagaagc catttgcctt tgcgggtcta tgggacgcat ggaaatcgcc cgaaggagaa
     1081 atacttctat ccttcactat catcacgaca acggctaacg agttgatgga gcctatacac
     1141 gacagaatgc cggtaatcct tcatgagaaa gacgaatcct tatggctaga cccggaattt
     1201 aaggatacca ataagctggc tgctctatta aaaccatttc catctgatat gatggaagcg
     1261 tatcgggtat ctacgattgt gaactctcct aagaatgact cgcctgattg catccgacct
     1321 gtcctgagcc aggtcgaagg gcctgtaaaa tgaattactc tatactaaac tttgatccga
     1381 gggagggtcg taaaatggac gtcattgaaa tgtacagatt aaaaagaggc gttaagctaa
     1441 atttaccgct tttattaagc cgcttacccg ctggctttcc ctcgccggcg gatgactaca
     1501 ttgaggagcg catagaccta aacaagaagt taataaagca ccctgaggcg acttacttga
     1561 tacgggtgat gggcgactct atggaaaagg caaggattta cgatggggat tatctcattg
     1621 tagaccgcgc gctagaacct gaacataacg atattgtact agcgatattt gacggcgagt
     1681 ttactgttaa acggttaatt aagaagatag ataggtggtt cctcgtagct gaaaacccta
     1741 agtatccccc ttttgaagta acgcctgaaa ccgattttga gatatgggga aaggtcacat
     1801 atatatttca tccagcggtg tgagagccaa tgcaaaagat aattggtctt attgactgca
     1861 ataatttcta cgtgagttgt gagcgtgtgt tcaaccctag gctgagaggg cgtcctgtag
     1921 ttgtgctctc taataatgac ggctgtgttg ttgctaggag cgaggaagcg aaagctctag
     1981 gcatacccat gggagcccct gcttttcagc atcaagcttt atttaaccag cataaagttg
     2041 aaacgctctc ttccaattac acgctctatt ctgatatgtc gcagcgtgta atgagcatcc
     2101 tctcaagatt cacgcctgag ctagagcttt actccattga cgaagccttt ttagaccttt
     2161 caggcttcac aaatttcaat ctcactgaat atgctaagga gatcaaagcc acagtttata
     2221 aatggactgg gatcccaatc agtattggaa taggccctac gaaaacacta gccaagctgg
     2281 caaacaagct tgctaagaaa aacaagatgt gtggcggagt gtttgatatt acagatcacc
     2341 ctcgaataga tgatttcctc tctagcgtga aagttgagga tgtttggggc gtaggtagac
     2401 aatacacgaa actgcttaac cgaaactgga tatctacagc cctggaccta aaaaatgctc
     2461 cggatatctg gataaaaaag aacatgtcta tagtgggact tcgtacagta tgggagctgc
     2521 gtggtatatc gtgtattgaa ttagaagacc tttccgagcc taataaacag attatacgtt
     2581 ctcgttcctt tggaaagcct gtgcaaactc tacaagagct taaagaggct gtaacatttc
     2641 atgcgacacg agcagccgaa aagctgagag agcaaaaatc agtcacgttt ttcattagtg
     2701 cctttatcga aacaaaccgt tttagaacag aagaacctca gtattcaaat agcgcaggtt
     2761 gctatttgcc tgagcctaca gcctacacac cgttgctaat caaaaatgcc cttgcctgct
     2821 tagagagaat atatagagaa ggatttaaat ttataagagc aggcgtagtg cttatggaca
     2881 tagtgccaga ggatcaaatc cagcttaatc tatttgtgcc ttcacgtcct ttagaaaagg
     2941 acaagtctat gatgaaagca gttgacagga tcaatgtcaa atggggaagc aactccataa
     3001 aatacgcctc ctcaggactt gagcaaccat ggagaatgaa aagagccaag ctctcgcctc
     3061 gctacaccac gcactgggat gaaatgttag tagttaaatg ttcgcctccc tgttatcttt
     3121 ctagagtcgc aacctcccac caataaacag cacaatatta ccaagattga gaaattgtat
     3181 tgactagaga agagtgtttg ttatatactg agagcatcga cgggtgttac gaggatttcc
     3241 gaataacact ccatataggg tgattatatg gaaaagattc cgtatgattg atagacgcag
     3301 atcgtcactt gaaaaggagg tgattctgag attacaaaaa cagtaaacaa cctcagctat
     3361 aacgaacaga ataagcttaa ggtgtctagg tttgcaagct tagacatatc ctacaacaat
     3421 cacaattcaa aaggaggtta ccatggctgg gagaaagttt gatccaaacc gtttgacgtt
     3481 tgagcaaggc atagagagga ttcagaaggc catcaaaaaa tcaggcgtgg agattgaggg
     3541 gcgaattgtc acctcacgcg atgaggggat gttcaagtta agagaactac tcaggaccga
     3601 caataaaccc aatggctttc aaaagtacca gttgccggtt gctcttagat gcgaatctca
     3661 aatgtttctt acagtggcag agcctggagc caaagcgcca cgtcacgcgc ataaagaagg
     3721 agatgggatc cgcttcattg tcgctggatc gatcatctac gacggtaaag aactgacctc
     3781 aaatgactgg atgttcattc cggcaggtgc gccatattca tttgaggttg ggccacatgg
     3841 tgcgactatg ttttattgtt attgctgctg ctgcgcctag tttcaaggat attgcgacca
     3901 gaactctgag gaagcgcctc tggtcgccga aactgcgcct aacacccgtt gcagcggagc
     3961 cgccgcaaca gaggcttcga ttatcaccaa tgttttagcg gcggcccgct gaaccaggcc
     4021 gttagccgct gatgaacttt gcgacaccga aaatagcaac atcaagagcg gaaggattct
     4081 gttacatatc gggctacggt aacagaacga tcaatttttg tagcccccta aatgaaaagg
     4141 agacaagaaa tggctacaaa aaaaacatct tctcagaaga accgtgaagc aaaaacattc
     4201 aaactagaca tcgctgtcca tcgaagaatt ctcaacttcc tgaatgaagc catccagcca
     4261 gaagatttgg tgtacgagaa agtgcccctt ccaaatcctg aagttgaccc tattcacgaa
     4321 gacaatcctg aggaactgaa actgaaacgt aaaaagatcc tggattttaa agtggcgaaa
     4381 gaggtcattg aattcagaga ccaagaatat ccgcttggat tccgaaattt gaaagaagtg
     4441 ctggacctta agctatttga ccgtcgacac ttggatgttt tgctgcatca tttcagtaac
     4501 atgttttacg gaagctggtc tgtttttcca caaagcattc ctcgcagggg gcccggtggt
     4561 tacgatggcg tagtccatgc agcactgtta cacaccggca aggttctgtt tatcacagca
     4621 gatgaaacga cgttgctttg gaatccggat gatacaacac ccgctacctt tgaggaccca
     4681 gttactcagc cccacctaac accggatgcc atgtcaggct actcggtgct atgcggtgga
     4741 cattcctttc tgtcggatgg gcgcctactg gttgttggtg gtggtggata tggaccccat
     4801 tctaaagcaa agtggggcta taaattcgac ccaacaagca ggacatggac aagaaccgcg
     4861 aacagcatgg tacatgatcg gtggtaccct acagttatga cattgggcga ccagcgaatc
     4921 gccaacagcc acgagatttt ggttgtctgc ggacatggca gcggtgatat ggaaatctat
     4981 gatgaggctt ccgacagctt tgtggaagtg acctcagggg acgacaagcc atttcctaat
     5041 ttgtaccccg gccttca
//
LOCUS       DATDRL010000298         3114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3006438, whole genome shotgun sequence.
ACCESSION   DATDRL010000298 DATDRL010000000
VERSION     DATDRL010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3006438"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VI935_10015"
     CDS             <1..770
                     /locus_tag="VI935_10015"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HLE25969.1"
                     /translation="RFKWKDSSFEVERNNGTWIGKDIPDYVDIDQSRVWAILRTFVNL
                     KIDNFEGDEPNNLAAHGLDKPIAEIDLFQYGKPVRVSFGNKKESGDYYVKLDLDNSAY
                     SVSEFVLRQIPESVNDIRVRRIVKLDAEKVRGVEINRGDNKISILKEGNKWKLGDDNS
                     KKVDESKVGELIADIRSLELEEFVDDNPKDLSPYGLEKPAVELSILESDKRRTVLFGK
                     TEKDKVYVKFADGKSVYLVGDNIISKIPSSSEELIKK"
     gene            complement(934..2724)
                     /gene="aspS"
                     /locus_tag="VI935_10020"
     CDS             complement(934..2724)
                     /gene="aspS"
                     /locus_tag="VI935_10020"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HLE25970.1"
                     /translation="MLELLGDWKRTDYCGSLGAEDLGHEVILMGWVQSKRDHGGLIFV
                     DLRDREGIIQVVFNPQLNIEAHQKAGLLKDEWIVGVKGTVTRRPQETINPNIKTGEIE
                     ITVKEIRILNTSKALPFPIENEIKVDELLRLKYRFLDLRRPMMKENLILRHEIAGATR
                     NYLNSKGFIELETPYLTRSTPEGARDFLVPSRLNPREFYALPQSPQLLKQTLMISGFD
                     RYYQIVRCFRDEDLRADRQPEFTQIDLEMSFVSEIDVMEIVEGMLKAIFEKAKGIEIH
                     TPFPRIRYDEAMLKYGNDKPDTRFGLELYDITEIFMNSGFKVFSDAIKRGGIVKALNL
                     KGKASDFSRKELDDLVEFAKSLGAKGLAWIRITGDEWQSPITKFLTETEKKVLQNTLK
                     TEDEDVIFFAADSAYTVNLVLSNLRLHLGERFNMIDQSKLSFLWVVDFPLLDFDETEK
                     RYVAMHHPFTSPKEEDLGLFDSTPEKVRARSYDIVLNGVEIGGGSIRIHRSDIQQKLF
                     DKIGLEPEEAKKKFGFLLEALEFGAPPHGGIALGLDRLVMLIAGADSIRDVIAFPKTQ
                     KGVCPLTEAPSPVDMKQLLELQIRVDLKEK"
BASE COUNT          906 a          572 c          644 g          992 t
ORIGIN      
        1 taaggtttaa atggaaagat tcgtcgttcg aagtcgagag aaataatgga acttggattg
       61 ggaaagatat tcctgattat gtagatattg atcaatcaag ggtatgggca attcttagga
      121 cttttgtgaa tttaaagatt gataattttg aaggcgatga gccgaataat ctcgctgcac
      181 atgggttaga taaaccgatt gcagagattg atctctttca gtatggtaaa cccgttcgtg
      241 tgtcattcgg gaataaaaaa gaatcgggag actattacgt aaagctcgat ttagataatt
      301 ctgcttattc cgtatctgag tttgtattaa gacagatacc tgaaagtgtg aatgacatta
      361 gggtaagaag gattgtgaaa ttggatgccg aaaaggtaag gggagttgag attaatagag
      421 gtgataataa gatttcgatt cttaaagaag gaaataaatg gaagctaggt gatgacaata
      481 gcaaaaaagt tgatgaatca aaagtcggtg agctgatagc ggatattagg agtctcgagt
      541 tagaagaatt cgttgatgac aatccgaagg atttgtctcc ttatggattg gaaaaacccg
      601 cggtagagct ttcgattttg gaatcggata aaaggagaac agttcttttt ggaaagacag
      661 agaaagataa ggtttatgtt aaatttgccg atgggaaatc ggtttatcta gtaggggaca
      721 atataatttc taagattcct tcttctagcg aggaattaat taaaaagtga gatgatgatt
      781 ataaacatag tttcaattca tatgatcagg aatttagtct tgttgctatt aagcaatccc
      841 ttgtagtatg agcgtcatcc tggcgcgata tcatcaaatg tatgtaggag cgtcatcttg
      901 acgcgacaac tatatcgctg ctggaagctg cacctacttt tctttcaaat ccactcttat
      961 ctgaagctct agaagctgtt tcatgtcaac cggtgaagga gcctcggtaa gcggacagac
     1021 tcctttctgg gtttttggaa aggcgataac atcccttatt gaatctgcgc ctgcaatgag
     1081 cattacaagc ctatcgagtc ctaaagcaat tccaccatgt ggaggggccc caaactccag
     1141 agcttctagc aggaatccga attttttctt tgcctcttcc ggttcaagcc caattttatc
     1201 aaagagtttt tgttgtatgt cacttctatg tattcttatg cttcctccac cgatttcaac
     1261 cccgtttaga acaatatcat aagaccttgc ccttaccttt tctggggtag aatcgaaaag
     1321 acccaggtct tcttccttag gcgatgtgaa tgggtggtgc attgcgacat aacgtttttc
     1381 agtttcatcg aaatcaagaa gagggaaatc tactacccag aggaatgaga gtttgctttg
     1441 gtctatcata ttgaatcttt ctccaagatg aagcctcaaa ttagaaagta caaggttaac
     1501 tgtatatgcg gaatcagctg caaagaagat aacatcttca tcttcggttt ttagagtgtt
     1561 ttgcaaaacc ttcttttcgg tctcagtgag aaactttgtt ataggtgatt gccattcatc
     1621 tccagtaatt cttatccatg caagcccttt tgctccaagt gatttcgcaa attcaactaa
     1681 gtcatcaagc tcttttctag agaaatctga ggcctttccc ttgagattga gtgcttttac
     1741 aattccgccc ctttttattg cgtcgctgaa cactttaaat cccgagttca taaatatctc
     1801 ggtaatatcg tatagctcaa gaccgaacct agtatcaggc ttgtcgtttc catatttgag
     1861 cattgcctcg tcgtatctaa ttcttggaaa tggcgtatgg atttctattc ctttagcttt
     1921 ttcaaaaata gctttaagca ttccttcgac gatttccatg acatcaattt cgctcacaaa
     1981 tgacatttct aaatcaatct gtgtaaactc aggctgacgg tcggctctta agtcctcatc
     2041 gcggaaacat ctaacaattt gatagtagcg atcaaaaccg gagatcataa gtgtttgttt
     2101 tagaagttgc ggcgactgag gaagtgcata gaattccctt ggattaagtc tacttggtac
     2161 aagaaaatcc ctagcccctt caggtgtaga ccttgtgaga tatggggttt ccaactctat
     2221 gaatcctttg gagtttagat aatttctcgt agcccctgct atttcatgtc tgagaatgag
     2281 gttctctttc atcattggtc ttcttagatc gagaaacctg tactttagcc ttaggagctc
     2341 gtcaacttta atttcgttct ctatagggaa aggaagagct tttgaggtgt taagaatcct
     2401 tatttccttt acggtaattt caatctcacc cgttttaata tttgggttta tagtttcttg
     2461 ggggcgtctt gttacagtgc cctttacacc tactatccat tcatccttga gtagccctgc
     2521 tttctgatgc gcttctatat tgagttgagg attaaaaact acctggatta tcccttctcg
     2581 gtctcttaaa tctacaaata taagcccacc atggtccctt tttgactgaa cccaccccat
     2641 tagaattact tcatgcccaa ggtcttctgc acctaggctc ccacaatagt cagttctttt
     2701 ccaatctcct aaaagctcaa gcatataaac tgcttcctat tatttctgat taatgttcaa
     2761 aaatgttaac ctttatagtg gatattggca agcaaagttc tatggggtag tgtgtgagtt
     2821 ggttaagaat agactggaaa aagcaaggtc ggtatgaaat acaatcctga tattcatcat
     2881 aggggggtct ataaggttaa aaggatacga ttattcacaa gttggagcat attttataac
     2941 gataggtgct cgtaataaag aattatattt tcaacaatac cctgaattaa agcggatttt
     3001 aattcggcaa tggcaagaaa taccagatag atatgcaaat ataagattag acgaattcgt
     3061 cattatgcca aatcatatac atgggataat aattgtaggg gcaacccttg cggt
//
LOCUS       DATDRL010000299         5195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_635440, whole genome shotgun sequence.
ACCESSION   DATDRL010000299 DATDRL010000000
VERSION     DATDRL010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635440"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VI935_10025"
     CDS             <1..478
                     /locus_tag="VI935_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25971.1"
                     /translation="FARYYASADNSSSGKLSGQVYESKDTIYEIGKLSDKWKRINIKG
                     GDLAFWFEAIQAAITVNSTCNIKANYSLKALSNSLLIGIRDKQMVEEKEITISGEKAL
                     ESIYFAKMDNDSVKLCAVVMKNNTCIYDFTYTSPPDKFDSGLSEFKNFLSQFRVIK"
     gene            478..1254
                     /locus_tag="VI935_10030"
     CDS             478..1254
                     /locus_tag="VI935_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLE25972.1"
                     /translation="MKEFITAIGKYFVSLVESLGDVGLLLYRTLRYSLTPPFNLRLIL
                     EQLDEIGFKSIPIVILSSLAIGMVMVLQLAYGFARFGAKGLVGPVVSLAIVRELGPVL
                     TSLLVGGRVGSGITAEIGSMKVTEQIDAIKTLGADPIKKLVVPRFIAALISFPFLSII
                     ADLVGIFGGMIIANTELGVTPRLFISSIIQQVGISDFISGISKTVFFGIIVAIVGCYI
                     GMKAEGGTQGVGRATTLTVVVSLVLIIIMDFFLTKLFLVF"
     gene            1338..1961
                     /locus_tag="VI935_10035"
     CDS             1338..1961
                     /locus_tag="VI935_10035"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HLE25973.1"
                     /translation="MWLKHEKGRQEEILQYWFGELKESEAPLEDYYEIWFGKRREVDE
                     FIRTNFEEDLKLAIEGKLKSWEDASRSTLALIILLDQFSRNIYRGTAKAFAHDSIALE
                     ICLRGIEKGFDTRLHPVERLFFYMPLEHSEDLEIQKKSVEYFFMMEKLYPTPLSLASM
                     ASEFRKYAEMHYVIIEKFGRFPHRNEILGRESTPEEIEFLKQPGSSF"
     gene            complement(2272..2586)
                     /locus_tag="VI935_10040"
     CDS             complement(2272..2586)
                     /locus_tag="VI935_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25974.1"
                     /translation="MGTDSLLIPYVYARSEYGRDEPGPGTVSVFSVVGNGVLTQIAGS
                     PFATGGFSLGAGLHLKPHYRKYNKAAIMSAASPSTLLLETSTPTISVKTQMRRMQILT
                     AI"
     gene            complement(2656..2991)
                     /locus_tag="VI935_10045"
     CDS             complement(2656..2991)
                     /locus_tag="VI935_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HLE25975.1"
                     /translation="MVQTFVYYVYIMSNKGLTTMYIGVTNNLERRVLEHKLGKGSKFT
                     TKYKLNRLIYFEEFEEVTNAIAREKQLKNWHRDWKWNLTKSLNPGLKDLSEGWYDDET
                     LKQVQGDNK"
     gene            3366..3788
                     /gene="ruvX"
                     /locus_tag="VI935_10050"
     CDS             3366..3788
                     /gene="ruvX"
                     /locus_tag="VI935_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HLE25976.1"
                     /translation="MRILALDVGTKTIGVAVSDELGIAAHGVTTIKRTDLKHDLEALR
                     KIIDQYKPSEILVGVPYNYAGTVSKRGEGILKFAEKIKTTFSLPVELWDESFSTVDAE
                     KILLEADLSRKKRKKVIDKMAAVFILQDYLVKKRSEES"
     gene            3972..4766
                     /locus_tag="VI935_10055"
     CDS             3972..4766
                     /locus_tag="VI935_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLE25977.1"
                     /translation="MPYARVKGIQIYYETYGEGFPVVLIGGLGSEIQSWATQIPIYSK
                     HLKVIVFDNRGAGKSDKPDVPYTIELMADDTAELLDTLGVKSAYVVGKSMGGMIGQWL
                     AIKCPEKVRKLVLGCTSASRDQVGNEILRMGRETATKIGMKAVWLTAIFWGYTREYIE
                     RNLSSIKEALAKVPENPEALTGYIRQSLACEGHNVTDLVGKIKSPTLVMLGEQDLIVS
                     PNRSCELAELIPGAELKVFKGIGHGFWRERQEEVDKLVLDFLLRDV"
     gene            complement(4848..>5195)
                     /locus_tag="VI935_10060"
     CDS             complement(4848..>5195)
                     /locus_tag="VI935_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HLE25978.1"
                     /translation="PYRLARVEILEDVHGTNENIYRERIVELILRWNRIIGKEHESHL
                     INIDTRLPLENLTDAIASTSVSNVFEKQELLEEINVQKRAEKILAHLETKIEVISIVA
                     KRRNQIVEKRNLN"
BASE COUNT         1557 a          909 c         1139 g         1590 t
ORIGIN      
        1 atttgctcgt tactatgctt ccgctgataa ttcttcaagc ggcaaactca gtgggcaggt
       61 atatgagtcc aaagatacga tttatgagat aggaaaactt tctgataaat ggaagagaat
      121 taatatcaag gggggagatc tagctttctg gtttgaagca attcaagcgg caataacagt
      181 gaattcaaca tgtaacataa aggcgaacta tagtttgaaa gccctttcaa attcgcttct
      241 tataggtatt agggataaac aaatggttga agaaaaagaa attactatta gcggcgaaaa
      301 agctctcgag agtatttact ttgcgaagat ggataatgat tcagtgaaac tatgtgctgt
      361 cgttatgaaa aataatacat gtatttatga tttcacctac acatcaccac ctgacaagtt
      421 tgatagcggg cttagtgaat ttaaaaactt tctatcgcaa tttagggtta taaaatagtg
      481 aaggaattta ttaccgcaat cgggaaatat ttcgtttctt tggttgagag tctgggtgac
      541 gttggattac ttctctatag aaccctacga tacagcttaa cccctccctt taacctacgg
      601 cttattttag aacagttgga tgagataggt tttaaatcca tccctattgt aattctctct
      661 tctttggcaa tagggatggt gatggtgctt caactcgctt atggatttgc aaggtttggc
      721 gcaaagggat tagttggtcc tgttgtttct cttgccattg tgagggaatt gggtccagtg
      781 cttacatctt tacttgtagg tggtagggtg ggctcgggga tcacggcaga aatcggctct
      841 atgaaggtta cagagcagat tgatgcaata aaaacccttg gagcagatcc cattaagaag
      901 cttgtcgttc cgagattcat cgcagcctta atctcttttc cttttctttc cataattgct
      961 gaccttgtag ggatttttgg tgggatgatt atagctaata cagaattagg agtaacaccc
     1021 agacttttta tttctagcat aattcagcaa gttggcatct cagattttat tagcgggata
     1081 tcaaagactg ttttttttgg gataattgtt gcaattgttg ggtgttatat cggaatgaag
     1141 gccgaaggcg gcacacaggg ggtaggacga gctacaacac ttacagttgt agtttcactt
     1201 gttcttataa taatcatgga ttttttcctt acgaaactgt ttctggtatt ttagtttaga
     1261 aggtaggggg ataatcgggc gcttgttgtc aaaggatatt tacgtacttt agaatttctg
     1321 tatggtgaag ttaaataatg tggttaaaac acgaaaaggg gcggcaggaa gagattctac
     1381 aatattggtt tggtgaactt aaagagagcg aagcaccctt agaagattac tacgaaattt
     1441 ggtttggaaa aagaagagag gttgatgaat tcataaggac caacttcgaa gaagatctga
     1501 agctagctat tgagggcaaa ttaaaaagtt gggaagatgc atcccgcagc actctggctc
     1561 ttataatcct gttagatcaa ttttcgcgta atatctatcg aggaacggct aaggcgtttg
     1621 ctcatgattc gatagccctc gaaatttgct tacgaggaat tgaaaaggga tttgacacaa
     1681 gactgcatcc agttgaacgg ttattctttt atatgccttt agaacactca gaggacttgg
     1741 aaatacaaaa gaaatccgtt gaatattttt tcatgatgga aaaactctat ccaactccac
     1801 tatctttggc ttcaatggca tcagaattca gaaaatatgc agagatgcat tatgttatta
     1861 ttgagaagtt tgggcgattt cctcatagaa acgaaatact cggacgtgaa tcaactcctg
     1921 aagagattga gttcttgaag caacccggct cttcgtttta gtgagtaatg ccatttccaa
     1981 tataaaaagt tgcgatagac gaggcttcgt cgtgagctca gccgaacggt tttccaattt
     2041 cgttatatca agcgggataa tgatgttgca agacgggact agaaagtccc gtctaccggc
     2101 attttggagt tctattaacg ataggcggtt ttctaacccg cctacgtcct tgtgtcccag
     2161 aatttgactg agttgtggcc aggggggcga agtctcgccc ttctgctcca tgtagaacgc
     2221 ctcataaagg ttgctattga actttcaggg ttgcggttgc agagccgcta gttatattgc
     2281 cgtcaatatc tgcatccgcc tcatttgtgt tttcacagat attgttgggg ttgaggtttc
     2341 cagtagaagg gttgatggag aagctgctga cattattgcc gccttattat atttgcggta
     2401 atgcggtttg aggtgaagac ccgcccctaa gctaaacccg ccggttgcaa agggtgatcc
     2461 ggcaatctgt gtcaaaacac cgttcccgac tactgaaaat acggaaactg tgccaggacc
     2521 aggctcatct cgcccgtatt ccgaacgggc ataaacatat ggaataagga gggaatcagt
     2581 ccccacattt tattattaaa tttatgggtg ctttttaaaa attattttac gaagaaatgt
     2641 tttactaaaa atcggctatt tattgtcacc ctgaacttgt ttcagggtct catcatcata
     2701 ccatccttca gataaatcct ttaaaccagg attcagagat tttgttaaat tccatttcca
     2761 atctctatgc caatttttca attgtttttc tctagcaatt gcattagtaa cctcttcaaa
     2821 ttcctcgaag taaattaggc ggtttaattt gtatttagtt gtaaattttg agccctttcc
     2881 taatttgtgt tctagtaccc ttcgttctaa attatttgta actccaatat acattgtagt
     2941 taatcccttg ttgctcatta tatatacata atatacaaat gtttgaacca taataaataa
     3001 tgtacaataa gagattctga aacgagttca gaatgacata aaatataaac tgacatttat
     3061 aagaaaataa catgctgtaa aactatgatt ttaatactag ttctattgaa cagaagttca
     3121 tccagaatgg aattgtcatc acagagactt aattgtgaaa ctacactcta aacaaaagga
     3181 gcacatagca attgccttgc ctgtattaaa tgagaatgaa gctaaacaat ttatttgaga
     3241 ttcttcattg cattctgaat gacacggagt gtagtattgc ttcgccaggg tgaaaacgca
     3301 gactgaagtc tataggtttc atgcaataac aaggttcatt attcgttata agagatagaa
     3361 cgataatgcg catattagcg ctagatgtag gtaccaaaac aatcggggtt gctgtaagcg
     3421 atgagcttgg gattgcggct catggggtta cgactataaa aaggacagac ttgaagcatg
     3481 accttgaggc tcttcgtaaa attatagatc aatacaagcc ttccgagata ctggtcggag
     3541 ttccttacaa ctatgctggc actgtaagta aaagaggaga ggggatatta aagtttgctg
     3601 aaaaaataaa gactactttt tcactacctg ttgaattatg ggatgagagc ttctccacgg
     3661 ttgatgcaga aaagattctt cttgaagcag accttagtag aaagaaacga aaaaaggtta
     3721 ttgataaaat ggccgctgtt tttatacttc aggattattt agtgaagaaa aggagtgaag
     3781 agagttgatt atatctaacc ctaaatttct gtaattgtca ttgcgagcgt tagtgaagca
     3841 atctcgatgt ttagattgct tcgtcgtttc actcctcgca atgacacaac caatgttctc
     3901 gctgcttttc gtgacactga attatggatc gtttcatggc tcactataat tcaatgtaac
     3961 aaagaaaaac tatgccttat gccagagtaa aaggaatcca aatatattat gaaacctacg
     4021 gtgagggttt tccagttgtc ctcattggag ggcttggaag tgagatccag agctgggcta
     4081 cccagattcc catttattca aagcacctta aggttatagt ttttgacaat cgtggagccg
     4141 gaaaatcgga taagcctgac gtgccttaca caattgagct tatggctgat gatactgcgg
     4201 agcttcttga tacccttggt gttaagtccg cttatgtggt aggaaaatcc atgggtggaa
     4261 tgataggaca gtggcttgcc ataaaatgtc cagagaaagt gaggaagctt gttttaggct
     4321 gcacatccgc ttcacgagat caggtaggaa atgagattct caggatggga agagaaaccg
     4381 caacaaaaat cggaatgaaa gctgtatggc tgacagccat attctggggg tatacaaggg
     4441 agtacattga aagaaaccta agctcgataa aggaagcttt agccaaagta cctgaaaacc
     4501 ctgaagctct tacaggttat attaggcaga gccttgcgtg tgaagggcat aacgtaactg
     4561 atttagtggg gaaaattaaa tctcctacgc ttgttatgct aggagagcaa gatttaattg
     4621 tttctccaaa tcggtcatgt gagctagcag agttgatacc cggtgcagag ttaaaggtat
     4681 ttaaagggat cggacacgga ttttggagag aaagacaaga agaagtagat aaattagttt
     4741 tagattttct gcttagagat gtttagccac ggatggacat gatgcatgat acaagataca
     4801 ggatacaaga taaatcatga atcatgtatc atggatcttg catcgtttta gttaagattg
     4861 cgcttttcta caatctgatt ccttctttta gcaactatag atatgacctc aatcttagtc
     4921 tctaagtgtg cgagaatctt ctcggctctt ttctggacgt tgatttcttc aagcagctcc
     4981 tgcttttcaa aaacgtttga aacactcgta gaagcaatcg catctgtcag gttctcaagt
     5041 ggaagccttg tatcaatatt tatcaaatga gattcgtgtt ctttaccgat gattctattc
     5101 catcttaaga taagctcaac tatacgctct cgataaatat tctcatttgt accatgtaca
     5161 tcttctagga tttcaacacg ggcgagtcta taagg
//
LOCUS       DATDRL010000300         3142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_367908, whole genome shotgun sequence.
ACCESSION   DATDRL010000300 DATDRL010000000
VERSION     DATDRL010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367908"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VI935_10065"
     CDS             complement(<1..351)
                     /locus_tag="VI935_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLE25979.1"
                     /translation="MSRFKRLLHIRDACLIVSVLVFVLTFGSHIGFADEATKVDPKIP
                     SYTKVSGISGNINSIGSDSMNNLMTLWCEGFNKFYPNVKCQIEGKGSSTAPPALIAGT
                     AQLGPMSRSMKGAEI"
     gene            complement(821..2590)
                     /locus_tag="VI935_10070"
     CDS             complement(821..2590)
                     /locus_tag="VI935_10070"
                     /inference="COORDINATES: protein motif:HMM:NF028046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLE25980.1"
                     /translation="MKAFWKHFLFNLFIISLVFFLFTILIIRELEKHDKALTEERLLT
                     EANLLREVLKTPITQGKKEEIKSLISEVGKKIEIRITVLDKDGVVIGDSEEDPARMEN
                     HAKRPEVIDAVSKGTGKSIRYSTTVKKDMFYVALPVENQTGEVEAIVRTALPLSFLEK
                     LFSPLKSKVIYLALILIFIAISLSFASSRALTKSLDKTIKLSEEIAKGNFNVSIPISD
                     KKGEIGKFNLALSTMAKKLDDLFKQVSLEKTQLEIVLSTMSEGVMVVRGDGTITLINS
                     ALKDMLGIKDDSIGKPYWEILRIREIMDLVEKVIKKQVAEKREISVFYPTERYFLTSA
                     IPLNSEVKEAIFVIFDITEFKRLERIKADIVANVSHELRTPLTAIKGYVETLEEGAYE
                     NPEERNHFLSIIKRHTDRLINIVSDLLLLSEIERKSAPWGEEPKAAFENVDFKEVVYS
                     SLEALKSRVEEKGINVSLSIKEDLPKYRGDRFLLEQMFINLIDNAVKYTPEGGTIGVQ
                     VNQTNSHFGIEVFDTGIGIPKDHLPRIFERFYRADKTRSRKLGGTGLGLSIVKHVVIM
                     HGGEIKVESEVGKGSRFIITFPV"
     gene            complement(2599..>3142)
                     /locus_tag="VI935_10075"
     CDS             complement(2599..>3142)
                     /locus_tag="VI935_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLE25981.1"
                     /translation="LVILDIMLPGADGLEICRMLKNNSRTSSLPIIMLTAKGTEADIV
                     VGLELGADDYIVKPFSPRELVARVKTILRRARKEDGADKLIRIGPLTIDSERFEAAVD
                     GKKVGLTTTEFKILEVLAEGRGRVYTRDQLLKKKRIWGDEKLIYDRTIDVHIKNLREK
                     LGKSGGMVKTIRGIGYKLEG"
BASE COUNT          886 a          684 c          498 g         1074 t
ORIGIN      
        1 tatttcggct cctttcatag aacgggacat aggtcctaac tgcgctgttc ccgcaattaa
       61 ggctggtgga gctgtgcttg aacccttgcc ttctatctgg cactttacat ttggataaaa
      121 tttattaaat ccttcacacc aaagtgtcat aaggttattc atactatctg aaccaatgct
      181 attgatgttt ccggatatac cgctgacctt cgtgtaagat ggtatcttag gatcaacctt
      241 tgttgcctca tcggcaaatc caatatgact tccaaatgta agaacaaaaa ccaaaacgct
      301 gacaattaag caagcgtctc ttatatgtaa caatctttta aatctactca tgactatgat
      361 cctccttgat gactaatttt cttttgtttg ttataaatta aaccttgaat tgttaagaaa
      421 ttgttaagag tccattaacc ttatgttaat atttgaaagc cgaatcaaag tttgatctat
      481 ctgaaaaata caaagcattt tactgtaatg atttatggat tgttctaccg attgactagt
      541 gtgtataaat catactgagc gataaagcac acaacacgac cagaacaaac cgttcatcct
      601 gtcgaaggat gcaaagtatt tatccaggtc gtaaatggcg cccttcagaa agccaatgac
      661 gagtggatta atacccgttg aatatctggg gcgactgagg aactttcatg agtttatcaa
      721 gggctcaagt ggatacgacg actactcaac tggatttggt attagatacc aaaaagtgat
      781 atatgcaaga caaactgtta tatattttcg aataaacctt tcacactgga aatgtaatta
      841 taaatctgct tccttttcct acttcacttt caactttaat ttctccaccg tgcattatta
      901 caacgtgctt tacaatgcta agcccgagac ctgtaccacc gagcttcctc gaacgtgttt
      961 tatcagccct gtaaaatcgc tcaaaaattc taggcaggtg atctttggga attccaattc
     1021 ctgtatcaaa aacctcgatt ccaaagtgtg aattagtctg attaacctga actcctatag
     1081 tacccccttc tggcgtatac tttacagcat tatctattag gtttataaac atttgttcta
     1141 aaagaaatct gtctcccctg tatttgggca gatcctcttt tatgctaaga gaaacattta
     1201 tgcccttttc ttcaaccctg ctttttagcg cctcaagaga cgagtaaaca acttctttaa
     1261 aatcaacatt ttcaaaggca gcctttggtt cttctcccca cggagcgctc tttctctcaa
     1321 tctcagacag caaaagaagg tcagaaacaa tattgattaa tctatcggta tgccttttta
     1381 ttatgctaag aaaatgattt ctctcttctg gattttcata agctccttcc tctagtgttt
     1441 caacatagcc cttaatggct gtaagcggtg ttctgagctc gtgagacaca tttgcaacaa
     1501 tatcagcttt tatcctctct agtcttttga actcagtaat atcaaatatc acaaatattg
     1561 cttcttttac ctccgaattt aatggaattg cacttgttag aaaatatctc tctgttgggt
     1621 aaaagactga aatctctctc ttctcagcaa cctgtttttt aataactttt tctactaaat
     1681 ccattatttc cctaattctt aaaatctccc agtatggttt tccaatcgaa tcatccttta
     1741 ttccaagcat gtcttttaag gcactattta taagagtaat tgtgccatca cctcttacaa
     1801 ccattacccc ttcactcatc gtgctgagaa ctatctcaag ctgagttttc tcaagagaaa
     1861 cttgtttaaa tagatcgtct aattttttag ccattgtgct taaggccaag ttaaactttc
     1921 cgatttctcc ttttttatct gaaataggga tacttacatt aaaattccct tttgcaatct
     1981 cttcagagag cttaatagtt ttatcaagtg attttgtaag tgctcttgat gaagcgaagg
     2041 aaagagaaat cgcaatgaaa attaaaatca aggcaagata aattaccttt gattttaatg
     2101 gtgaaaatag cttctccagg aagctaagtg gaagtgctgt gcggactata gcctcaactt
     2161 cacctgtttg attttctaca gggagtgcca cataaaacat atccttctta acggttgtgc
     2221 tatagcgtat gctttttcca gttcctttag aaactgcgtc tattacttct ggtctctttg
     2281 catggttttc catcctagca ggatcctctt cagaatcacc gatcacaact ccatccttat
     2341 ctaatactgt aatccttatc tctatttttt tgccaacttc tgaaataagg gattttatct
     2401 cttccttttt gccttgtgta atcggagttt taagaacctc acgaagcaag ttcgcttcgg
     2461 ttaataacct ttcttccgtt agtgccttgt catgtttttc gagttcacgg attataagga
     2521 tagtaaatag aaagaaaact aaagaaatga taaatagatt aaaaagaaaa tgcttccaaa
     2581 acgctttcac atttttccct acccttctaa cttgtagcct ataccccgta ttgtttttac
     2641 catgcctcca gacttaccta gtttttctct gagatttttt atatgaacat ctatcgttct
     2701 atcatagatt agcttttcat ctccccaaat cctcttcttc ttaagcaatt gatcccgtgt
     2761 gtataccctt ccccttcctt ctgcaagcac ctcaagaatc ttaaactcag ttgttgttag
     2821 cccaactttc ttaccatcaa ctgcagcctc aaatctttct gaatcaatcg tcagaggacc
     2881 gattcttata agtttgtccg ctccatcttc ttttctcgct cttcttagta tggtctttac
     2941 tctggcaaca agctcacgtg ggctaaatgg ttttactatg taatcgtctg caccgagttc
     3001 gagtcctaca acaatatcgg cttctgtgcc ctttgcggta agcatgatta ttggtagtga
     3061 tgatgtgcgg ctgttatttt ttagcatcct acagatttca agtccatccg ctcccggaag
     3121 cattatgtca aggatcacaa ga
//
LOCUS       DATDRL010000301         4760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3270659, whole genome shotgun sequence.
ACCESSION   DATDRL010000301 DATDRL010000000
VERSION     DATDRL010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3270659"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..476)
                     /locus_tag="VI935_10080"
     CDS             complement(282..476)
                     /locus_tag="VI935_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25982.1"
                     /translation="MPILREKTFEDPIITTPEERLKIFWQEDHFRIYGLDNKQRLEKA
                     GFSVKVDSYLTELSEDAVNR"
     gene            complement(600..995)
                     /locus_tag="VI935_10085"
     CDS             complement(600..995)
                     /locus_tag="VI935_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25983.1"
                     /translation="MDYLIRLVKGIVPEQYHPTLGEIYRIFRSFLYIGNKFARPCCVW
                     RFSVFLPFGVKPRPIAQFQRCGSLERHRLLWLNLKNRTNPFSDNLKVLHFTPEYIFQK
                     TFRFLPNLDYISADLDSPLAMVKVDITNI"
     gene            complement(1036..2982)
                     /locus_tag="VI935_10090"
     CDS             complement(1036..2982)
                     /locus_tag="VI935_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HLE25984.1"
                     /translation="MIRIGIDTGGTFTDFVFYDGKNVQTYKVQSLPSNPSKALLKGLK
                     DGLGGGNRNIEIVHGTTVATNALLERKGAKVALITTKGFEDVIEIGRQNRGKLYDLFW
                     EPREPLIKRELRFGLNERTNFQGKVIKNVRLADVKKISSKLKKLKVGGIAISFLHSYA
                     NPKNEERVEDLLKPLGIPISTSSKILPEFREYERTSTTVANCYLLSRVKSYMDELSKK
                     LYASRLFIMQSSGGVVSPIQAGEEPVRLLFSGPAGGAVGGFKIAETIGHKKVITYDMG
                     GTSTDVALYDGALRFTTETNIDGIPIKIPMVDVMSIGAGGGSIAYIDQGGALKVGPMS
                     AGADPGPACYGKALDATVTDANVVLRRINPRWFLGGSMEIFPEKSRAALVRLSDKLKL
                     SIMQLAEGIIKVANSNMERALRVISIGRGFDPREFALLSFGGAGGLHACELALGLGIQ
                     TVIFPREPGTLSALGMLMADSFKDYSLTTFLDAEKVTPNAIEKGFKVLESKARKELPG
                     EQLRFERFLDTRYKRQSHEITIPYNRDFVRTFHQTHKKMYGYHKLESDIEIVTLRVRA
                     RIEKEKIELPRLTTTPKEVESKKEKLFYREKEIGVNSHKRHDFFPGFTFSGPALILES
                     TSTLFITQDFRCEVDEWGNIVARV"
     gene            complement(3073..3663)
                     /locus_tag="VI935_10095"
     CDS             complement(3073..3663)
                     /locus_tag="VI935_10095"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HLE25985.1"
                     /translation="MKESLILSFIPLFVAMDPIGVVPLYLTLTGELNHHEKRKVVKDS
                     VLTACVLGVVFVFGGKVIFQVLGISVADFAIAGGLLLFSISILNLVKDINPEINRKAI
                     GSLGIFPIGTPIIVGPAVLTTLIILVDNHGFVPTLISFGLNLIILGAALLKADVIFSV
                     LGEGGARGFAKVMHILLAAIGIMMVRRGIMELISGG"
     gene            complement(3660..3884)
                     /gene="rpoZ"
                     /locus_tag="VI935_10100"
     CDS             complement(3660..3884)
                     /gene="rpoZ"
                     /locus_tag="VI935_10100"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HLE25986.1"
                     /translation="MARITIEDCLEKVENRFALAVAAMKRAKQIVKGAKPLSEETDNK
                     SVVLALREIAGENVKVVYPPGYTKINPPRK"
     gene            4270..>4760
                     /gene="hisIE"
                     /locus_tag="VI935_10105"
     CDS             4270..>4760
                     /gene="hisIE"
                     /locus_tag="VI935_10105"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HLE25987.1"
                     /translation="MNIKDIRFDEKGYVPVIVQDAKNGQVLMFAYANQDALEKTIKTK
                     KTHFWSRSRKKLWEKGEESGNVQEVKDVFLDCDKDTILVLVNQKGVACHTGKRSCFFE
                     NIHGKEASAPSFGPTRTGKTLGEVYQVIEDRKRNPREGSYVSSLFKKGLDTILKKVGE
                     EAS"
BASE COUNT         1449 a         1012 c          874 g         1425 t
ORIGIN      
        1 ttattttacg tattttcaca aaatcattta taggaatttc tcttatcagc ataatggttt
       61 tctctcttgc cgatctataa aactattctt tttcaaaccc gcacgaggca atttcgggct
      121 aacgcgtagg acctataacg ttaggagcga aggggttatt ttgcaaaacc tctgtgtaaa
      181 cacaataatt gtaatcccgc cccgtaaaat tttcaattgc tcttatcagg tttagaacaa
      241 aaatatatat tctcttccaa tacgccttca ggcacgagac tttatcggtt tacagcatct
      301 tcgcttagtt cagtcaaata agaatccacc ttcacgctga aacctgcctt ttcgagcctt
      361 tgcttattgt ctaatccata aatacgaaag tgatcttctt gccaaaaaat ttttaaacgt
      421 tcttccggcg tcgtgatgat tggatcttca aaggtcttct ccctcaaaat aggcacttga
      481 agaatccctc atccgccagg ctttagaact ctaaataaca ccgtcaaagc cttttggtca
      541 tcaacaacat gttccagcat atgactacat caaattgcat caaaggaatt gtcttcgtat
      601 taaatatttg taatatccac ttttaccatt gccaaaggtg agtctaaatc agcactgata
      661 taatcaaggt taggcaaaaa tctgaaagtc ttttgaaaaa tatattccgg tgtaaaatgt
      721 aacactttga gattatcaga aaagggattg gttctatttt taagatttag ccataataaa
      781 cggtgcctct caagagaacc gcatcgttgg aattgggcaa ttggtctcgg cttaacacca
      841 aacggtagaa agacggaaaa gcgccaaacg caacaaggac gggcaaattt gttgccgata
      901 taaagaaatg accggaagat tctataaatt tctcctagag tgggatggta ttgttccgga
      961 actatacctt ttacaagtct aattaaatag tccatagatt ctttttcgtt ctttagagaa
     1021 aattatatat taatctcaaa ccctcgctac tatatttccc cattcatcta cttcacaccg
     1081 aaaatcctgg gtaatgaaaa gtgtagatgt gctttccaaa attaaggcgg gaccagaaaa
     1141 ggtaaacccc ggaaagaaat cgtgtctttt atgggaattt accccaatct ctttttccct
     1201 ataaaacaat ttttcctttt ttgattctac ttcttttgga gttgtagtta atctaggaag
     1261 ctcgattttt tctttctcaa ttctagccct aactctcaaa gtcactatct ctatatcact
     1321 ctccagtttg tggtaaccat acattttttt gtgggtttga tgaaatgtac gaacaaaatc
     1381 tctattgtag ggaatggtta tctcatgaga ttgccttttg taacgtgtat caagaaaacg
     1441 ctcaaacctc agttgctctc ctggtaattc tttacgcgct ttactctcta aaaccttgaa
     1501 gcccttttca atggcgttag gggtgacctt ttccgcatct aaaaaagttg taaggctgta
     1561 gtccttaaaa gaatctgcca ttaacatacc aagcgcagaa agcgtcccag gctctctcgg
     1621 aaaaattacg gtttgtatac ccaagccaag agcaagttca caagcgtgta gtcccccagc
     1681 accaccaaaa gaaagaagcg caaactccct agggtcaaac ccccgtccaa ttgagataac
     1741 cctgagtgcc ctttccatat ttgagtttgc aacctttatt atgccctcgg caagttgcat
     1801 tatagagagt tttaacttat ctgaaagtct taccaaagct gctcttgatt tttctggaaa
     1861 gatctccata cttccgccta gaaaccatct tggatttatt cttctgagaa caacgttagc
     1921 atccgttaca gttgcatcga gggcttttcc atagcatgca gggcctgggt ctgctcctgc
     1981 actcataggc ccaaccttta aagcccctcc ttgatcaata taagcgatag aaccgccacc
     2041 cgcacctata ctcattacat caaccatagg aatttttatc ggaattccat caatattagt
     2101 ttctgttgta aatcttagtg ctccgtcgta gagagctacg tctgtagaag taccacccat
     2161 atcataggta attactttct tatgcccaat tgtctctgca attttaaacc cgccaacagc
     2221 tcctccagcc gggcctgaga aaaggagcct cacgggctct tcaccagcct gaattggcga
     2281 aactacacct ccacttgatt gcataataaa gagccgactc gcgtaaagtt tttttgaaag
     2341 ctcatccata tacgacttaa ccctggaaag taaatagcag tttgctacag tggttgaggt
     2401 tctttcatat tccctgaact caggcagaat ttttgacgaa gtggaaatag gaattccaag
     2461 gggttttaga agatcttcaa ctcgctcttc attctttggg tttgcatatg aatggagaaa
     2521 agagattgca attccgccga cttttagttt cttgagtttt gaagagattt tcttaacatc
     2581 agctaatcta acatttttta taacctttcc ttggaaattg gttctttcat tgagcccaaa
     2641 cctaagttct ctttttataa gcggttctcg tggctcccag aaaagatcat ataacttccc
     2701 cctattctgc ctgcctattt caatcacatc ctcaaaccct ttagttgtga taagggccac
     2761 ttttgcccct tttctttcaa gaagcgcatt cgtggcgacg gttgtaccgt gtactatttc
     2821 tatgtttctg tttcctccgc ctaggccatc ctttaaccct tttaatagcg ctttagacgg
     2881 atttgaaggg agtgattgta ccttataggt ttggacattc ttcccatcat agaatacgaa
     2941 atctgtaaaa gttccacctg tatcaattcc tatccgaatc atgataatag ccatcagcat
     3001 attagccgtc agctatcagt tgaaagcctt aattgatatt ttaccgattg ccgatgactg
     3061 gtagctagat tgctaacccc ctgaaatcag ttccataatt cctcttctta ccatcataat
     3121 cccgatggct gcaagtagga tgtgcattac ctttgcgaaa cccctggctc caccttctcc
     3181 aagcactgag aatatgacat cggcttttaa aagggccgcc cccaaaatta tgagattcaa
     3241 tccaaaagaa atgagggttg gaacaaatcc atggttatcc acaagtataa taagagttgt
     3301 tagcacagca ggtccaacga taataggtgt tccaatagga aagataccaa gagaaccgat
     3361 tgcctttctg ttaatctcag ggtttatatc tttaaccaag ttcaatatgg atattgagaa
     3421 tagaagtaaa ccacccgcta tcgcaaaatc agcaacactt attcctaata cctgaaaaat
     3481 aaccttccct ccaaaaacaa acacaacccc aagaacacat gcagtcagaa ccgaatcctt
     3541 tactaccttt ctcttttcat gatggttcag ttctcctgtt agagtaagat acaaagggac
     3601 aacacctatt ggatccatag caacgaaaag ggggataaaa ctaaggatca aagattcttt
     3661 catttacgag gtgggtttat ctttgtgtaa ccgggaggat aaaccacctt tacattttct
     3721 cccgcaatct ctctaagtgc tagaacaaca gatttgttgt cggtttcttc agaaagaggc
     3781 tttgccccct ttacaatttg cttagccctt ttcatcgctg ctactgcaag ggcaaatctg
     3841 ttttcaacct tttctagaca atcttctata gttattcttg ccatagttat ttacccctca
     3901 gattaagtaa attaatatta aaatataagc tacataaagg aaatagtaaa gagttcaaga
     3961 ctcggttagt attttacata gtgtgctgta aacgatccat aatttatttt gtcattgctt
     4021 ggaacaaaac cccttaccta atgcgaggag aattatttcg agagccgctt tctttgaacc
     4081 tgccttatct ctatcgcagt gtgatagaga tgggaataaa tagtgtggct caaagggaaa
     4141 cacaaagaac gaggaatgga aataaaaggc gttgactaag catgtcctga gtgaaaacga
     4201 tggatgaatg tgttcgcaac aataattatg gaaagttatg aagctaacta taaatttgga
     4261 gggatgtaaa tgaacatcaa agatataaga ttcgatgaaa aagggtatgt gccggttatt
     4321 gttcaagatg ctaaaaacgg gcaagtacta atgttcgcct atgcaaatca agatgcgctt
     4381 gaaaaaacca taaaaacgaa aaaaacccac ttttggagta gatcaagaaa aaagctctgg
     4441 gaaaaggggg aagaatcagg aaacgttcag gaagtaaagg atgtttttct agactgtgac
     4501 aaagatacta tactagttct ggttaatcaa aagggcgttg cctgccacac cggtaaaagg
     4561 agttgttttt ttgagaacat tcatggaaaa gaagctagtg ccccatcatt tggacccaca
     4621 agaactggta aaaccttagg ggaggtgtat caggttattg aagatagaaa acgaaaccct
     4681 cgagaaggct cttatgttag cagtctcttt aaaaaggggc tcgataccat actcaagaaa
     4741 gttggagagg aagctagtga
//
LOCUS       DATDRL010000302         5899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3020311, whole genome shotgun sequence.
ACCESSION   DATDRL010000302 DATDRL010000000
VERSION     DATDRL010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3020311"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VI935_10110"
     CDS             complement(<1..222)
                     /locus_tag="VI935_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25988.1"
                     /translation="MSAYFRIKKSAILAGVQVKNGLRLAMIFNSFYLSPNLFSENQRA
                     FWKRTALMSLSRIEEFLFGHTLKVGQTQSE"
     gene            595..1665
                     /gene="amrS"
                     /locus_tag="VI935_10115"
     CDS             595..1665
                     /gene="amrS"
                     /locus_tag="VI935_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HLE25989.1"
                     /translation="MKEALFYERLEENRVLCTLCSHLCRIKDGKKGVCGVRINWGGKL
                     YTLVYDRVVARDIDPIEKKPLFHFYPSKRAYSITTVGCNFRCFYCQNFEISQYPKGVH
                     WHEKDINPEEPEPICPLLEKMIPGEKVTPEQIVDAAKASGCDMIAYTYTEPTIFYELA
                     YDTAKLAAEEGLKNIFVTNGYITKEALIAIQPYLHAANIDLKGFTDRFYKRICGAKLQ
                     PVLESIRAYKELGIWIEVTTLVIPNLNDSEEELRQIAEFIKGVGEEIPWHVTQFYPAY
                     KLIDQSITPITTLRKARDIGLRVGLRYVYEGNVPGEGGENTYCYNCKKLIIERYGFSV
                     LKNIIENSHCPYCCTTIDGIGL"
     gene            complement(1766..2689)
                     /gene="rpoH"
                     /locus_tag="VI935_10120"
     CDS             complement(1766..2689)
                     /gene="rpoH"
                     /locus_tag="VI935_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HLE25990.1"
                     /translation="MVENKESLEKEKEEKEKVSKYLNKSLPAPTDSVKKYMAEISRYP
                     ILSREEEYKFAMRYRKKGDLEAAKKLITSNLRFVIKIANEYRNYRINLMELIQEGNLG
                     LMQAVKKFDPTRGYRLITYAVWWIRAYIQSYIIKTWSLVKIGTTQAQKKLFYKLGSAK
                     RKMELTNKILSPEDYKTLAKELGVSDKAIAEMEQRMAGKDVSLDVEIREEGERATHLD
                     FLADKLASQEEILTKIQEEEKIKRELDSALKSLEERERFIIENRILRDNPLTLEELGT
                     KLNISRERVRQVENEALKKMKTILKNMGVSG"
     gene            3365..4516
                     /locus_tag="VI935_10125"
     CDS             3365..4516
                     /locus_tag="VI935_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLE25991.1"
                     /translation="MELKNINLEENELGEFIQEEEVFASHEIGTSSMLDEPEPGSEFE
                     PEELESIEDEESQDVPDEQVRLLFAYFKDLGAESLFTSKREIEISAKIKKCEAKIRET
                     ETLIAKLSKKRASMIKNDVRKKLTKRINRLNAFTKAYSEKAKELKKRFAKANLRLVVS
                     IAKRYIGRGLPLPDLIQEGNIGLMRAIEGFDHTKGFKFSTYAVWWIHQAIIRALLDQT
                     RTIRVPVYVLEKASKVKRIRSMLHKQMGRRPTPEEIAEKSGIPVEGVKRILDAEGDVV
                     RLDSHIVNGEKTTLLELIPDKRLPAPDSVVAHASLNVRISEALSILTLREKEIIMLRF
                     GINQVSTHTLDEIGRKFDLSRERIRQIERDALKKLETSGFRDTLKSFLE"
     gene            4518..4664
                     /locus_tag="VI935_10130"
     CDS             4518..4664
                     /locus_tag="VI935_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25992.1"
                     /translation="MKKREQVKIRKPVAKKPTRVILSKKDKMKRKRIKHRNLIKPTYM
                     LEGI"
     gene            4963..5175
                     /locus_tag="VI935_10135"
     CDS             4963..5175
                     /locus_tag="VI935_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25993.1"
                     /translation="MKKICILYNPSGNPVREIKLKGKKKLAQEGRVFEAEPTIESFTI
                     GNERNCDFRNLREAHESLFGYTCVGW"
     gene            5361..5606
                     /locus_tag="VI935_10140"
     CDS             5361..5606
                     /locus_tag="VI935_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE25994.1"
                     /translation="MRTKWYVDAEIDRYSIPYFECCLRDVCNNFGISELKKTGHMETD
                     GFYHKSEFFFPDSIDEESFDDMLNDLLASGEPDYYQE"
BASE COUNT         1962 a          997 c         1153 g         1787 t
ORIGIN      
        1 ctcagattga gtttgaccca ctttgagtgt gtgtccaaag agaaactctt caattcgaga
       61 taaggacatt aaagcagttc ttttccaaaa ggctctttga ttttcggaga atagatttgg
      121 gcttaaataa aaagaattaa aaatcattgc caacctcaac ccatttttca cttgtactcc
      181 tgctaatata gctgattttt ttatcctaaa gtatgcggac atgatatcta ggtttgccta
      241 ctgtagttgc agatgaagca ccctctttat aaaagagggt tccgaaaatg gctttcaagg
      301 caaaacaaac ttttttcaat aaaatagaat tcttgctgat atgcaatctc acttagttgc
      361 ataatagatt taagcttaaa ataggtttaa ctcttgtttc ggaaaataag tgtcaaacat
      421 cagagaatct gacaaatata ccttcttaag aagaatgcac catttatatg atccttaaat
      481 atttcctcaa gttatgtgaa ttaaacaagt ggcataaagt ttgctaaata aaacagacta
      541 aagattttag atgatcgcat aattaacgta actaatggta aaaaatctaa gaagatgaaa
      601 gaggctctat tttatgaaag actagaagaa aatagggtgt tgtgcaccct ttgcagccat
      661 ctgtgtagga taaaggatgg caaaaaaggg gtatgcgggg ttaggataaa ctggggtggg
      721 aagttatata cgcttgtata tgatagagtt gttgcacgag atattgatcc tatagagaaa
      781 aagccacttt ttcacttcta tcctagcaag agagcttact caataactac agttggatgt
      841 aactttagat gtttctattg ccagaatttt gaaatttctc agtatcccaa aggtgtacac
      901 tggcatgaaa aagatattaa tcctgaagaa ccagaaccaa tctgtccgct tctagaaaag
      961 atgattcctg gggaaaaggt aacaccggag caaatagttg atgccgctaa agcttcaggc
     1021 tgtgacatga tagcatatac ctacactgag ccgaccatat tctatgaatt ggcttatgat
     1081 accgctaaac tagccgccga agagggactt aaaaatatat ttgttaccaa tggatatatt
     1141 actaaagaag ctttaatcgc tattcaaccc tacctccatg cagctaatat agatttaaaa
     1201 ggctttacag ataggtttta taaaaggatc tgcggagcaa agctccaacc tgtcctagag
     1261 tccatcaggg catacaaaga gctcggaatt tggattgagg ttacaacact tgttatacct
     1321 aatcttaatg actcagagga ggaattaagg caaattgctg aatttataaa gggtgtggga
     1381 gaagaaatac catggcacgt tactcagttt tatcctgctt ataagcttat tgatcagtcc
     1441 ataactccta ttactaccct tcgtaaagca agagatatag gtttgagagt gggtctcaga
     1501 tacgtatatg aaggaaatgt tccgggtgaa ggaggagaga atacttattg ctataattgt
     1561 aaaaagctaa ttatagaaag gtacggtttc tctgttttga agaatattat tgaaaattct
     1621 cattgcccct actgttgcac aacaatagat ggaataggat tatagaaata atcacccgat
     1681 cttaaggaaa taaagtatcc acgtcgccaa aatacctcag attctattta acgaaaaata
     1741 gttgtattaa tttgagttgc taaaatcacc cacttacacc catgtttttc aagatcgttt
     1801 tcatcttttt caatgcttcg ttttcaactt gtcttaccct ctctctcgaa atgttgagtt
     1861 ttgtaccaag ttcttcaagt gttagtgggt tatccctaag aatcctattc tctattataa
     1921 acctctccct ctcctccaaa gatttaaggg cggagtctaa ttccctttta attttttcct
     1981 cctcctgaat ctttgtgagt atctcttcct gactcgctaa cttatctgca agaaaatcca
     2041 gatgagttgc cctttctccc tcttccctta tttcaacatc aagtgatacg tctttgccag
     2101 ccattctctg ttccatttcg gcaatagctt tatctgaaac cccaagttcc ttagcaagag
     2161 ttttatagtc ctcaggactc aagattttat ttgtaagctc catcttacgc ttcgcagagc
     2221 ctagtttata gaaaagcttt ttttgtgcct gtgttgttcc gatctttaca aggctccagg
     2281 tttttataat gtagctctgt atgtatgccc ttatccacca gacagcataa gttataagac
     2341 ggtatcccct tgtcgggtcg aacttcttta cagcctgcat aagtccaagg ttaccttcct
     2401 ggattaattc catgagattt atacggtagt ttctatattc gtttgcaatc tttatcacaa
     2461 accttagatt tgaggtaatg agcttttttg ccgcctctaa gtctcctttt tttctatatc
     2521 tcatagcaaa tttatattct tcttcccggc tgagaatggg gtacctgctt atctcagcca
     2581 tatacttctt aactgagtct gtcggagcag ggagtgattt attaagatat ttgctcacct
     2641 tttccttttc ttctttttcc ttttctaagc tttctttatt ttcgaccatt atattaaatc
     2701 cccaagatat agaatgctta ataccttaga agcaagttct atgccatagg taaacagtat
     2761 aataataaag acagataagg tgagatgtaa aaccactagc gtactacacg ctatgtcagc
     2821 tgtcttaaaa taagacaaat agctttattt gaaagaaggt tgtgggatag ttatttaaga
     2881 cccactaggg aagaaatagc ttaaaatttg aacttaaata gatttgttgt tttgttacga
     2941 aggaatagga acgatatttg ctgattttac attagcaatt gcgtacaata aaagcaagct
     3001 tcccagattc caccaaaatg aataacttac gtaccgcaat tgcgaaaaat atgcttgaca
     3061 aaccctttta atagaattat gttaatatta tacaggtctg agggggagag aggggataaa
     3121 aatggaataa atgggaattg cttattggct tttaataagc atcgccttaa attaattata
     3181 gtactttcta aagaaattac tccctgcaat tatccttaat ttcatgttat gcaaggaagt
     3241 agttagaaaa cttctagcta ttctatagaa acaaagggta agagacgaga ttagaagcca
     3301 atagagaagc gtttgttccc ttgattcatt atttgtaaac aaattaacaa ataagaggta
     3361 aagaatggag ctaaaaaata taaatttaga agaaaatgaa cttggagagt ttattcaaga
     3421 ggaagaggtt tttgcttctc atgaaattgg aaccagttcc atgttagacg agccagagcc
     3481 cgggtctgag tttgaaccag aggagcttga atctatagaa gatgaggaaa gtcaagatgt
     3541 tccagatgaa caggttagac tcctctttgc ctactttaag gacctaggag ctgaatccct
     3601 tttcacatcg aagagagaga tagaaatttc tgcaaaaata aagaaatgcg aggcaaagat
     3661 aagggagacg gagacgctta tcgcaaaact ttcaaagaaa agagctagta tgatcaagaa
     3721 tgatgtgaga aaaaaactta caaagcgaat aaacaggctt aatgctttca caaaagcata
     3781 ttcagaaaag gcaaaggagc taaagaaaag gtttgctaaa gcgaacttaa ggttagttgt
     3841 gagtatagct aagagataca taggccgcgg actcccactt ccagacctta ttcaagaagg
     3901 aaacataggt ttgatgaggg caatagaagg atttgaccat acaaaggggt tcaagttttc
     3961 aacttatgca gtatggtgga ttcatcaggc tataattagg gcattgcttg atcagacaag
     4021 aacgattcgg gtacccgttt atgtgctcga gaaagcaagc aaggtaaaga gaataagatc
     4081 aatgctacat aaacaaatgg gcagaagacc aactcctgaa gaaattgctg agaaatctgg
     4141 aatacccgta gagggtgtta agcggatttt agatgcagaa ggcgatgtcg ttcgacttga
     4201 ctcacacata gtaaacggag agaaaacaac ccttctcgag cttatacccg ataaaagatt
     4261 acctgctcca gactctgttg tagctcatgc gtcacttaat gtgagaataa gcgaggctct
     4321 ttcaattctt acacttagag aaaaggagat aattatgttg agatttggaa ttaatcaagt
     4381 aagcacacac acacttgatg agattggaag gaagtttgac ctatcgcgtg aacgcattag
     4441 acaaatcgaa agggatgcac ttaagaagct tgaaacatct gggtttagag acactctaaa
     4501 gagctttctc gaataaaatg aaaaagagag aacaggtaaa gataagaaaa cccgttgcca
     4561 aaaagccaac aagggtgatt ttatccaaaa aagataaaat gaaaagaaag cgaataaaac
     4621 acaggaactt gataaaacca acatatatgc ttgaaggcat ataaaccttg tgagcatctc
     4681 tgccactcta tttcaccttt ttgataaggg cattaaagaa ctaacactgt ttctttagtg
     4741 cactttttta tcttcccttc gcaaataact tgcaagtaaa atcacttttt aatatgtaac
     4801 ctgtaagatg tccgattatc agacattaaa acgcctgccc gataattgta taatatgaga
     4861 accaaaaaaa atcagttctt ataatggcct acatttattt atcttttact aataagttgg
     4921 cacatttttt gtatgaatat atatacgatg tggtttatta agatgaaaaa gatatgtata
     4981 ctttacaatc cttcaggtaa tcctgtcaga gagatcaaac taaaaggcaa aaaaaaatta
     5041 gctcaagaag gtagggtttt cgaagccgag cctaccattg agtccttcac aataggtaat
     5101 gaaagaaatt gtgatttcag gaatcttaga gaggcgcatg agtccttatt cggctatacc
     5161 tgcgttggct ggtagtcgag taaactaata aggaaggggt tacatagaag atcggaaaaa
     5221 ggatttaaga gaccttttaa aattctattt gtctgatgtg ggtaattctt acgaatagaa
     5281 taaaacgtca ctttccttct gaagcattta caaagttatc ctttttcttt ttgaattttt
     5341 gagatattgg gaggtgtttt atgagaacaa aatggtatgt agatgcggag atagatcgtt
     5401 attcaattcc ttactttgag tgctgtctaa gggatgtatg taataacttt ggaatctccg
     5461 agttaaagaa aaccggtcac atggaaactg atggttttta tcacaagtcc gagtttttct
     5521 ttcctgatag tatagacgag gaaagcttcg atgatatgct taatgattta ttggctagtg
     5581 gtgagcctga ttattaccaa gaataaatgt gtcaccaaaa tagtaaaata gtagctagaa
     5641 ttttatataa aaccttatat accccttctc cctttctcgc agttggagag cattatcatg
     5701 attttaggca agtatctgat gatggagtta aggaatacct agaggaagct aaaactggag
     5761 atcaattcca taaggaggta agattatgaa aatagagttt attgggaaag gcattgatat
     5821 atcggaggat cttaagagtt atgttgagca taagcttttt acttttgaac gccatttgaa
     5881 ggaggttgga gagggcgag
//
LOCUS       DATDRL010000303        15172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2744508, whole genome shotgun sequence.
ACCESSION   DATDRL010000303 DATDRL010000000
VERSION     DATDRL010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15172
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2744508"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VI935_10145"
     CDS             <1..405
                     /locus_tag="VI935_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLE25995.1"
                     /translation="TDLSELTSISLDFAIDIANRFNSTIYLLHTLELHHSYEISTSKI
                     IEELRRLALKELRSYLNKIPKEKTKGINIQEMVTLYLRAWFGIVNFAEENGIDLIIMT
                     THGRKGLARFMLGSTAEKVISESPCPVLTIKP"
     gene            550..1602
                     /locus_tag="VI935_10150"
     CDS             550..1602
                     /locus_tag="VI935_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018879012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HLE25996.1"
                     /translation="MRANVFKGANKIGLEERPIPKAGPGEAVVKVTLTTICGTDVHIM
                     KGEYPVKVGQTIGHEPVGVIQELGIGVNGYKLGERVAINAITPCGQCEYCLSANWAQC
                     GGFLGGWKMGNTIDGCQADYVKIPNAMANLTKIPDELSDEDVLLTTDIFSTGLSGPES
                     GNIQVGDIVAVFGQGPIGLSATAGAKLKGAALIITVDTLPSRIKMSKKMGGDIVLDYK
                     KVDPVKEIMKITDGRGVDVAIEAIGLQESFENCIKCTRVGGTISSLGVYPAHLGNIGV
                     PLQDFGFGIADKKIVSTLCPGGKERMRRLISLVKNERVNLRQLITHRYSLDEIEEAYR
                     IFSNQLDGVIKVAIKP"
     gene            1695..2141
                     /locus_tag="VI935_10155"
     CDS             1695..2141
                     /locus_tag="VI935_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLE25997.1"
                     /translation="MKIQRILFPTDFSVYSEKAREYTLYLGEKLKASIYIIHAIEPLE
                     YPEIDEEIKKFYKELEKQMEKKIEDERETFEKKGVQAKTNIIIGPRWRVINTFAKEKD
                     IDLIVMGSHGLKTETGQMAIGTTSHKVAFSSPCPVLLVRYDEWAKK"
     gene            2122..3525
                     /locus_tag="VI935_10160"
     CDS             2122..3525
                     /locus_tag="VI935_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HLE25998.1"
                     /translation="MNGQKNRKKFLIEKNSKRMSDVVKQYEGWWATKPVRVKEGLLFS
                     YYAPSAKDIFLAGDFNGWKKRHTPLIKGKDDVWRIILELKPDRSYDYKYIVDGNWVND
                     PNNEDLNPDVAGGANSVIYLGSSGDILPKAHPERHKFTLEGRQIYNGSYFSSKYNQKF
                     EFHYISPQYEDGEKLPVVICFNNYIKSQELHIYARQNRYLVVIPHVTLGGQYIRQGKL
                     DVFPELLGFIKQSFTIDEDHVYVTGMSYGGLEALLVSLYYPDLIAASAVVFGPYRLRS
                     YKDTIEKMSKEELGQFLDSLDYPHKMLNNLKDFPLYISHGGGDEAIPFDEALTLHEIV
                     KKLGAPTELNSYPEHGHTWYMVDEDLPRVFDWFRRFKRNRFPKSINYTAPNGFLKNSI
                     FWLDFSPLQIEHPIKIEANINENNRLKLTIENIKRIKLKPSSKILKLPGVLYIDTHQG
                     TQQVDIQEEDKEIQLTF"
     gene            3572..4477
                     /locus_tag="VI935_10165"
     CDS             3572..4477
                     /locus_tag="VI935_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLE25999.1"
                     /translation="MKVKFMEQELISIPFGKSKIDALFYHTTAQSDRSEKEPIVIHVH
                     GFLGNFLDGSQRFLPPILAKAGYSSLSINTRMANFSLFFGFGILEDTVPQIDTAVVFL
                     KELGYKKIILSGYSLGGCIVLRYAALRNDPSAYPSLKGVIALATPRSMPDSIRRRWDR
                     WKSEPSYEEVYKRAKEFLKPDPYNASEDRTILIYKARGDTYRPEHTEIYTYKTWWFLA
                     GPEADGATAYKQIEKIKIPILLTQGWHDDVIDPIETFELAQSALDAGNRDVSAFYLNT
                     GHTLERKEDELGDIIIKWLDRRFQK"
     gene            4504..5391
                     /locus_tag="VI935_10170"
     CDS             4504..5391
                     /locus_tag="VI935_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLE26000.1"
                     /translation="MIRKNLISFQTKDSFQQDAFLLLRSSNNKLDVLNTPIIIHIHGV
                     LGHFLARGTPRLLPQALLEHGINSFSVNTRMAFMGQIMGVGIFDDIINDIDASLDLLK
                     KEGFSKIFILGYSLGANLVAYYASKRSDPCIHGLILEGCAFSLPEAQRKRWEKWISEP
                     SYDEVYSKAKEVLEPDPYRSSKDQIFIVYRAWGPTLNPFHIEIFTYKTWWFMRGPEAY
                     NAMTYRLISNIKVPILFLQGENDDILGAWEATELARLVREAGNSDVTVRYIPKAMHDC
                     MENPDEIVRTIVDWISQFT"
     gene            complement(5514..5957)
                     /locus_tag="VI935_10175"
     CDS             complement(5514..5957)
                     /locus_tag="VI935_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLE26001.1"
                     /translation="MEKILLVDDETNIRLFYKEVLCEEGYEVLEAESGKESFDILRRE
                     PIDLVVLDIKLRSESGLDVLQNLTKEFPNLPVVLCTAYSSFQDDYISWLAEAYIVKST
                     DTEELVKEIKRVINKKRRAGGDTLITKVEMKKKEDLQIVSKTTYC"
     gene            complement(6171..6416)
                     /locus_tag="VI935_10180"
     CDS             complement(6171..6416)
                     /locus_tag="VI935_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26002.1"
                     /translation="MRPIIVRTHSGKYEVKYDIVECYKYWLKEEKAPPKCCYCMLHNG
                     HVAVVNTEEEAKRLVSLLPECPGLPAMINSGGVLKTD"
     gene            6721..6921
                     /locus_tag="VI935_10185"
     CDS             6721..6921
                     /locus_tag="VI935_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26003.1"
                     /translation="MKKQLMRRRKEIIPKKKLTSEMVIEKLDSIITRLDIIEKDLREL
                     KGIVQPGSEEEKKHTPEFFMEK"
     gene            7089..7439
                     /locus_tag="VI935_10190"
     CDS             7089..7439
                     /locus_tag="VI935_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26004.1"
                     /translation="MKRIKSKERNALTSRMEWYFDEATEKKSTGHSETEKRLKERSKL
                     SPHMEYYFDEITDMKYIRCPVTGRKATIEVIRSASRDDTPFIDVAYCSIFGCAPTCKK
                     QCLKQINYMKHLHV"
     gene            7818..11399
                     /gene="nifJ"
                     /locus_tag="VI935_10195"
     CDS             7818..11399
                     /gene="nifJ"
                     /locus_tag="VI935_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HLE26005.1"
                     /translation="MRNRILAPIDGNEAVASVAYRLNEVIAIYPITPATPMGEWSDSW
                     ATEGRPNIWGTVPLVVEMQSEAGAAGTVHGALQTGSLATTFTASQGLLLMIPNMYKIA
                     GELTTTVFHVAARTLATHALSIFCDHSDVMATRATGFALLCSASVQEAMDFALISQAA
                     SLESRIPFLHFFDGFRTSHEISKVELLSDDELRMMIDEDLVLAHRARGLSPDRPVLRG
                     TTQNPDVFFQSRERTNPFYINCPDIVQKVMDKFAKLVGRAYRLFEYNGAPDAERVIVV
                     MGSGAEVVHETVDHLNNRGEKIGVVKVRLYRPFDTKRFIEALPSTTQAIAVLDRTKEP
                     GSSGEPLYLDSVTAIHEGIMGGWGKLKSIPKIIGGRYGLSSKEFTPAMVKAIFDHISI
                     EKPKNHFTVGIHDDLTHTSIAYDPKFSTEPDDVFRALFYGLGSDGTVGANKNSIKIIG
                     KNTNYHIQGYFVYDSRKAGAVTISHLRFGRRPIRSAYLIDSANLIGCHQPVFLERYDM
                     LKHIVPGGTFLLNTPYGPEEIWENLPISVQKQIIERKIKLYVIDAYKVARRSGLGGRI
                     NIVMQVCFFAIFKILPKDRSIEEIRRSLFETYGKQGEEIVHMNLQALDNTLENLFEVK
                     IPDSADSPIEIPPPVAEQSPLFMREVLGKMIAGQGDELPVSALPPDGTYPTGTTKWEK
                     RNLALEIPVWEPEICIQCGKCTIVCPHAVIRIKAYDPKYLEGAPDKFKSSYARDEVWK
                     GLKYTIQVAPEDCTGCGMCVDICPARSKSETRIKALNMYPQPPLREEESKNWDFFLTI
                     PETDRIHLHPMSVRHQQVQQPLFEFSSACAGCGETPYIKLLSQLFGDRLIVANATGCS
                     SIYGGNLPTTPWTTNAEGRGPAWSNSLFEDNAEFGLGFRLSIDIQREFAANLLKKVSP
                     VIGENLAHAILNAEQKDEADIYEQRKRVEALKKWLQDVESQDARRLLSIADMLVKKSV
                     WIIGGDGWAYDIGFGGLDHVLASGHNINILVLDTEVYSNTGGQMSKGTPLAAVAKFAS
                     AGKPTPKKDLGLIAMTYGDVYVASVAMGAKDEHTIRAFLEAEAYEGPSLIIAYSHCIA
                     HGINMTTAMRNQKAAVDSGQWLLYRFNPERAKQGENPLHLDSQAPKIPVQQYLYMENR
                     FKMLLKTRPIEAKRLFKEAQQEINSRWSLYEYLAARTKIGNREEKSK"
     gene            11472..12470
                     /locus_tag="VI935_10200"
     CDS             11472..12470
                     /locus_tag="VI935_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HLE26006.1"
                     /translation="MDLSTTYLGMTLRTPLVPSASPLSEEIDNIKRMEDAGASAIILY
                     SLFEEQLQLERYELHHHLTHHTDSFAEALTFFPEPTEFHVGPESYLYHIQKAKEAVSI
                     PIIASLNGTSAGGWIDYSKKIEQAGADALELNIYYIPTDMELTSEQVEQTYVEILKAV
                     KSEVKIPVALKLSPFFNNIAYMAKRLDDAGANALVLFNRFYQPDIDLENLEVQPNIIL
                     SSPQALRLPLRWIAILYGRVKADLAATSGIHTARDVIKMLMVGANITMLCSVLLRHGI
                     EHIRVIEQGVSNWMEEHEYESVRQMQGSMSHKNCEDPSAFERSQYMRGLLTYKPKA"
     gene            12674..12937
                     /locus_tag="VI935_10205"
     CDS             12674..12937
                     /locus_tag="VI935_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose 1,6-bisphosphatase"
                     /protein_id="HLE26007.1"
                     /translation="MIEPFLRLENKSVLERQNLADGRLHGLEDPKSPMGEHILIDYFA
                     SSCWDYVWHEAMEKAHYIRRHGPFMPPTLPPEEMGYTTKGRSS"
     gene            13129..14313
                     /locus_tag="VI935_10210"
     CDS             13129..14313
                     /locus_tag="VI935_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HLE26008.1"
                     /translation="MKRNIFVRSLTGRSPHEGDVEIVERKGLGHPDTICDNVAEEVSI
                     ALSKYYLDEFGAVMHHNVDKGLLVGGISNPSYNGGEIVEPIELTIVGRAIKEKRERNL
                     PVEEIAIEAMHKWLKENIRHLDTENQIITKVKIRPGSKGLIDLFERFREGKVPLSNDT
                     SIGTGFYPLDELEKTVYKVERLLNGAEIKRKYPFIGEDIKVIAIRNREKIRLTVAMAT
                     VDKYILSLNDYIDKISMVKKLLVKEISLGSNLEVEINTADKYEDESIYLTVTGTSGEH
                     GDDGQVGRGNRVNGLITPYRPMSLEAAAGKNPISHIGKIYNLFANDLSKAIVETGLAD
                     EAYVYIASEIGKPINQPQVLDIGVKGKKTDNEAITTIAEEMLDEMPNLWKKVIEGKYQ
                     IA"
     gene            14610..>15172
                     /locus_tag="VI935_10215"
     CDS             14610..>15172
                     /locus_tag="VI935_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HLE26009.1"
                     /translation="MRNGIRKRKVVIMGAAGRDFHNFNIFFRNKKDYEVIAFTAAQIP
                     NIEKRIYPPELSGGLYPKGIPIYPESGIKEILEKNKVDEAILAYSDLSNEEVMEKASI
                     VLSSGADFRLMGPYSTMLKSKKPVISVCAVRTGAGKGVTTRKIIRFLREKGYKTVVVR
                     HPMPYGNLKKQICQRFSKYEDLEKNDCT"
BASE COUNT         4923 a         2789 c         3245 g         4215 t
ORIGIN      
        1 acagacttat cagagcttac ctcaatttca ttagactttg ccattgatat agctaataga
       61 ttcaattcaa caatatacct tcttcataca ttagagcttc atcacagcta tgagatttca
      121 acaagtaaaa ttatagagga actgcgccgt ctggctctaa aagaattgcg gtcttactta
      181 aataagattc ccaaagaaaa gacaaaaggg attaacatac aagaaatggt gaccttatac
      241 ctgagggcgt ggttcggaat tgtaaatttt gcagaggaaa atgggatcga tcttataata
      301 atgacaacac acggaagaaa gggccttgct cggtttatgt taggaagtac tgctgaaaaa
      361 gtcataagtg aatctccttg ccctgtatta acaattaagc cctgaaagag aataacggcg
      421 aaactttctt tcatcctaga acctgataga ttatgcatac tatcctgata gtattgctcg
      481 cactcctata tggcatgact attgctaata ttttatgagg aagattttta aggaggtgtc
      541 aaaatgaaga tgagagcaaa cgtatttaaa ggtgctaata agataggtct tgaagagcgt
      601 cctattccaa aagcaggccc gggtgaggct gtcgttaaag tcactttaac aacaatttgt
      661 ggtacagatg tacatataat gaaaggagaa tacccagtga aagttggaca aaccataggt
      721 catgaacctg ttggagtaat acaagaactt ggaataggag tgaatggata taaattaggt
      781 gagcgagtag cgattaatgc gatcactcct tgcggtcaat gcgaatattg cctttctgca
      841 aactgggccc agtgcggggg gtttttgggc ggatggaaaa tggggaatac gattgatggt
      901 tgtcaagcgg attatgtcaa gattccaaac gctatggcaa atttaaccaa gattcctgat
      961 gaactttctg atgaggatgt tcttcttaca acagatatct tctcgaccgg tctttccggt
     1021 cctgaaagtg gcaatataca agtaggagat attgttgcgg tatttggaca aggacccatt
     1081 gggttaagtg ctactgcggg ggctaagctt aagggtgctg cgttgattat taccgttgat
     1141 actctccctt caaggataaa aatgtccaag aaaatggggg gagacattgt actcgattat
     1201 aaaaaggtag atccagttaa agagataatg aaaataactg atggtagagg agtcgatgtg
     1261 gcaattgagg caattggact tcaagaaagc tttgagaatt gtataaagtg cactcgagtt
     1321 gggggaacaa tatcgagtct tggtgtttat ccagcgcatc tcggaaacat cggcgtacct
     1381 cttcaggatt tcgggtttgg aatagctgat aagaagattg tttcgaccct atgtccgggt
     1441 ggaaaagaaa ggatgagaag attgatctct ctggttaaaa atgaaagagt taatttgaga
     1501 cagcttatta cccacagata ctcgcttgac gagatagaag aggcgtacag gatattttca
     1561 aatcaactag atggagttat taaagtagct attaaaccat aaaagtctgc attatattct
     1621 aggaaataaa ctctttgtgt cttatgatat ttgataatgt aagttagaat tcaaataaag
     1681 cgaggaggta catgatgaaa atacagagaa tactatttcc tacggatttt tcagtgtatt
     1741 cagaaaaggc aagagaatac actctttatt taggagagaa acttaaggct agtatatata
     1801 taatccatgc tatagaaccc ttggagtacc ctgagataga tgaggaaatc aaaaaatttt
     1861 ataaggaact cgaaaaacag atggagaaaa agatagaaga tgagagagag accttcgaga
     1921 aaaagggggt tcaagccaag acgaatataa ttataggacc acgctggcgc gttattaata
     1981 catttgcaaa ggaaaaagat attgacttga tagttatggg ctctcacggg cttaagactg
     2041 agaccggtca aatggctata ggaactacga gtcataaagt tgcattttca tcaccatgcc
     2101 ctgttctatt agtaagatac gatgaatggg caaaaaaata gaaagaaatt tttgatagaa
     2161 aagaactcga aacggatgtc ggatgtagtt aaacaatatg agggttggtg ggcaacgaaa
     2221 ccagtccgtg ttaaagaagg gctacttttt agttactatg caccctcggc taaggatatt
     2281 tttcttgcag gggattttaa tggatggaaa aagagacata cccctttaat aaaaggaaag
     2341 gacgacgtat ggcgtatcat tttagaacta aaaccggata ggtcctacga ttacaaatac
     2401 attgttgatg gaaactgggt aaatgaccca aataatgagg acctaaatcc agatgtcgca
     2461 ggtggcgcca actcagttat ttatcttgga tcaagtggag atattctgcc aaaggctcat
     2521 ccagagcggc ataaatttac gcttgaagga cgacagattt acaatggctc gtatttttct
     2581 tcaaagtata accagaagtt tgaattccat tacatatctc cccaatatga ggatggtgaa
     2641 aaacttcctg tggttatatg ttttaataac tatattaaat ctcaggagct ccatatatat
     2701 gctaggcaaa acagatacct tgtagttatc cctcatgtca ccctaggtgg acagtacatc
     2761 cgtcagggta agttagacgt attcccagag cttctaggat ttattaaaca aagcttcacg
     2821 atagatgaag accacgtgta cgttaccgga atgtcatatg gaggattgga ggctttactt
     2881 gtttccttat actatccaga cctaattgcc gcttcagcgg tggtatttgg accttatcgc
     2941 ctcaggtctt ataaagatac aatagaaaag atgtctaagg aagaattggg acaattttta
     3001 gatagtttgg attatcctca taagatgcta aataacttga aagattttcc gctgtacatc
     3061 tctcatggag gcggggatga agccatacca tttgatgaag cacttactct gcatgagata
     3121 gtaaaaaagc ttggggcacc aacagagctt aactcctacc cagaacacgg tcacacatgg
     3181 tatatggtag acgaggattt gcccagggtc tttgattggt ttaggagatt taaaaggaac
     3241 aggtttccca agagcattaa ctacacagcc cctaacggtt ttttaaaaaa cagtatattc
     3301 tggctagatt tctcacctct tcaaatagaa catccaatca agatcgaagc taacattaat
     3361 gagaataacc ggcttaaact cacaattgaa aatataaaaa gaataaaatt aaaaccaagt
     3421 tctaaaatac taaagctgcc tggtgtgctt tacatagaca cacatcaagg aacacagcag
     3481 gtggatattc aagaagagga taaagagatt caactaacat tctgaaatag atctctaact
     3541 ctttttataa tattcaagta gggatactta gatgaaagtg aaatttatgg aacaagaact
     3601 tatatctatt ccctttggca aaagtaaaat tgatgctctt ttttaccata ctacagctca
     3661 aagtgatcga tctgaaaaag agccaattgt tattcatgta catggtttct taggaaactt
     3721 tttagatgga agtcagcgtt ttttgcctcc gatcctggct aaggcaggtt attcttcgtt
     3781 atcaataaat acgcgcatgg cgaatttcag cctttttttc ggatttggaa tactagaaga
     3841 cacagtacct caaatagata cggctgtagt atttttaaag gagctagggt ataagaagat
     3901 tatactatcg ggctatagcc taggcggatg catagtactt agatatgcgg ctttgcgaaa
     3961 tgatccatct gcctatccaa gcttaaaagg ggtgatcgcc cttgcaactc cacgttcaat
     4021 gccggactcg attcgtcgcc gatgggatag atggaaaagc gaaccttctt acgaggaggt
     4081 ttataaaaga gcaaaagagt ttcttaagcc agacccatac aacgcatccg aagaccgcac
     4141 cattttgatc tacaaggcgc gaggagatac atatagacca gaacataccg aaatctatac
     4201 ttacaagaca tggtggttcc ttgcaggacc tgaggccgat ggcgcaacgg catataagca
     4261 aatagaaaaa ataaagattc cgattcttct tacccagggt tggcacgatg atgtaataga
     4321 cccaattgag actttcgaac tagctcagag tgcccttgat gccggcaata gagatgtgtc
     4381 agccttttat ttgaatacag ggcacacctt ggaaaggaag gaagatgaac taggagatat
     4441 aataataaaa tggcttgata gaagattcca aaagtagaac ctcgttttcc tagcatttaa
     4501 attatgatcc gtaaaaatct tataagtttt caaactaagg acagctttca acaggatgct
     4561 ttcttacttt taaggagttc caataataag ttagatgtat taaatacacc tataatcata
     4621 catatccacg gcgtgcttgg acattttcta gcccggggta ctcccaggct gcttccacaa
     4681 gccctcctag agcacggaat taactccttc tcagttaaca cccgaatggc ctttatgggg
     4741 cagataatgg gagtgggtat tttcgatgac attataaatg atatagatgc atctctagat
     4801 ttactcaaaa aagaaggctt cagtaaaatc tttatcctgg gatatagctt aggagcaaac
     4861 ctagttgcct attatgcctc aaaaagatcc gacccttgta ttcatggctt aatcctagag
     4921 gggtgtgctt tttctcttcc cgaggctcaa agaaaacgtt gggagaagtg gattagtgag
     4981 ccgtcctatg atgaggtata tagcaaagcc aaagaggtct tagaacctga tccttatagg
     5041 agttccaagg atcaaatatt catcgtttac cgagcctggg gacctacgct caacccgttt
     5101 catattgaaa tctttacata caaaacctgg tggtttatga gagggccaga ggcttacaac
     5161 gccatgacat acaggcttat atcaaatata aaagtcccga ttctttttct ccaaggtgaa
     5221 aatgacgaca tactcggagc atgggaagcc acagagttag cccgtctggt acgtgaggca
     5281 gggaattctg acgtaacagt gagatacata ccgaaagcta tgcatgattg catggaaaat
     5341 ccggatgaga ttgtaagaac tatagtagat tggatctctc aatttacata aataaattaa
     5401 gagaaagaga aataaacctt aacccagtct cacatgtatc cgaacttatt aatcgaatat
     5461 tggttatcaa tcgttgtgac tggtagatat ttctgctact tatgtgtatt tatttaacaa
     5521 taagtggttt ttgaaactat ttgtaagtct tctttcttct tcatttcaac cttcgttatg
     5581 agtgtatcgc ctccggccct tctctttttg tttatcactc tttttatctc ttttaccaac
     5641 tcctcagtgt cagttgactt tactatatat gcctctgcaa gccatgatat ataatcatcc
     5701 tggaaggatg agtaggctgt acaaagcaca actggaagat taggaaattc tttcgtcaaa
     5761 ttttgaagta catcaagacc actttctgat ctaagcttta tatctaacac gacaaggtca
     5821 atcggttcac gtcgtagtat atcaaaagac tcttttcctg attcagcttc taagacttcg
     5881 taaccttctt cgcataatac ctctttgtaa aaaagtctaa tatttgtttc atcgtctact
     5941 aataggattt tttccataat tcaccccctt ctcctagaat taaacttgat ttgctgctcg
     6001 tacataagtt gcttaagcct cttttccagt taaacatgct tattaaaact agaataagcc
     6061 taatcaatac acttatttct catccaccct ttcctcaacc ctagttcgtt tatcgattag
     6121 ttattattta aaagtttaat acctgtcaaa tatcagagaa tcagacactt tcaatctgtt
     6181 ttaagtacac caccgctatt aatcatagct ggcagaccgg ggcattcagg aagcaagctc
     6241 acaagcctct tagcctcttc ctctgtatta actacagcca catgaccgtt atgaagcatg
     6301 cagtagcaac acttaggggg tgccttctct tcttttaacc aatacttgta gcattcaact
     6361 atgtcgtatt tcacctcata tttacctgaa tgagtcctta caattattgg acgcatctat
     6421 tcaccttgcc ttcgtataat tttttctatt taaaaatatg catactctgt gacaaaggtt
     6481 attaaatata gaggcaaata ttatgccaaa gacataatgt atagattata tatacttatt
     6541 gaagcattaa gatattagga tgttgtcaga tgtcttaaat tcttacatgg attctcgttt
     6601 aataataaat ttatcgttag aatttgttga tatttaaaga agttacgtat tgaattttgt
     6661 ggcatgcagt ttgctgtata taaaaagagt gtgattaagt gataagaaaa ggtaatttga
     6721 atgaaaaaac aattaatgag acgtaggaaa gaaattattc caaaaaagaa gttaacctcg
     6781 gagatggtta tagaaaagct cgatagtatc atcacaaggc tggatattat agaaaaagat
     6841 ttgagagaac ttaaagggat tgtgcaacca gggagcgaag aggaaaagaa acatacacca
     6901 gaatttttta tggaaaaata ggcatggatt cataaataca aaaaggattt ccacatgaag
     6961 gtaatgaaaa agagtttgac aagacggtat aagcggcatt tatcgaaggt aaggttgata
     7021 atttaggact tttctctctt caaatgcaag gtcatgtaat taaatttaaa aaaaaggagg
     7081 gcaagaaaat gaaaagaata aaatcaaagg aaaggaatgc cttaacttct cgcatggagt
     7141 ggtactttga tgaagctacg gagaagaaat ctaccgggca ctccgagaca gaaaaacgat
     7201 taaaagaaag aagtaaatta tctcctcata tggaatacta ttttgatgaa attactgata
     7261 tgaaatacat taggtgtcct gttaccggaa gaaaagcgac tatagaagtc attagatcag
     7321 cttcgagaga cgataccccc ttcatagatg ttgcatactg ctctattttt ggttgtgccc
     7381 ctacttgtaa gaagcaatgc ttaaagcaga taaattatat gaaacacctt cacgtttaat
     7441 aaaaagatat attcctatta gatcctccat cccatctatc tgaaggcatt agagataaaa
     7501 taatctaatt ccttttatct attcgatttt tgtcctattt tcttacatct gacagaggtt
     7561 tagatttaag gatgtaaatt agctcaaggt aaaagtaaat gcgttataat aagatttgga
     7621 ataagataat ttggcacata aattgccatt aaaaagacag ggttaatagg caatgaaatt
     7681 gataatggca taagaagaaa ttttctttgt tctcatgctc tcgacggcac tagagaaaga
     7741 gaactttttc atcattatca aagagatatc catttatagc agcgaagacg gagacaaaga
     7801 agggctagta taaacgaatg aggaatagaa tccttgcacc catagatggc aatgaggcag
     7861 tagcttcggt tgcatacaga ttaaacgaag ttattgctat ctatcctatc acccccgcaa
     7921 ctcctatggg tgagtggtct gactcatggg caactgaagg gaggccaaat atctggggta
     7981 ctgttccttt ggtagtggaa atgcaaagcg aggcaggcgc agccggaaca gtgcatggtg
     8041 ctttgcagac tggttcactt gcaacaacat ttaccgcttc acagggactt ttgcttatga
     8101 ttcctaacat gtataagatt gctggggaac tgaccactac agtatttcat gtagcagcac
     8161 gaaccctggc tacacatgca ctttctatct tttgtgacca cagtgatgtt atggccacac
     8221 gtgccacagg ctttgctcta ctttgttccg catccgttca ggaagctatg gactttgcac
     8281 ttatttcaca agctgcaagc cttgagtcac gtataccatt cttgcacttt ttcgacggtt
     8341 ttcgtacatc gcacgaaata tcaaaagtcg agctactatc tgacgatgag ctacgtatga
     8401 tgatagacga ggatttggtt cttgcacatc gtgctcgtgg gctctcccct gatcgccccg
     8461 tccttcgcgg cactacacag aatcccgacg ttttttttca atctagagaa agaacaaatc
     8521 cattctacat taactgtcca gatatagttc aaaaagtaat ggataagttt gctaaattag
     8581 ttggccgggc ttataggctt tttgaatata acggagcccc tgatgctgag cgcgttattg
     8641 tggttatggg ctctggggct gaagtagttc atgaaacagt tgatcatctc aataaccgag
     8701 gcgaaaagat tggcgtagtt aaagtacgtt tgtaccgtcc gtttgataca aagcgtttta
     8761 tagaagctct tccttctaca acacaggcga ttgccgttct cgatcgcact aaggaacctg
     8821 gaagttcggg tgaaccatta tatctggact cggttactgc aatacatgaa gggataatgg
     8881 gcggatgggg aaaactaaaa agcattccta aaataatagg tggtcgctat ggcttatctt
     8941 caaaggaatt cacaccggct atggttaagg caatttttga ccatatttcc atagaaaaac
     9001 ccaaaaacca ctttactgtc ggcatacatg atgacctaac ccatacaagc atcgcatatg
     9061 accctaagtt ttctaccgaa ccggacgatg tatttcgcgc gctcttttat ggtcttggtt
     9121 cagatggaac cgtaggagcc aataaaaact ctataaagat tattggcaaa aacacgaact
     9181 accatataca gggatacttc gtttacgatt caagaaaagc cggtgcggta acaatttcac
     9241 accttcgctt tggtaggcgt cctattcgtt cagcctatct tatagatagt gcaaatttga
     9301 taggatgcca tcaaccggta tttcttgaac gttacgatat gttgaagcac atcgtgcctg
     9361 gggggacatt tctcctaaat actccatatg gaccagagga gatttgggaa aatcttccca
     9421 tttctgtgca gaaacagatt atcgaaagga agattaagct ctatgtcatt gatgcctata
     9481 aagttgcacg aagaagtggg ttgggaggac ggattaatat agttatgcag gtttgtttct
     9541 tcgctatctt caaaattctg cctaaggacc gatctataga ggaaattcga agatctctct
     9601 ttgaaacata cggaaaacaa ggagaggaaa tcgttcatat gaatcttcaa gcactggata
     9661 acaccttaga aaacttattt gaagtaaaga ttccagattc cgctgatagc cctattgaaa
     9721 ttcctccacc tgtggcagag caatcacctc tatttatgag agaggtttta ggaaaaatga
     9781 ttgcaggaca aggagacgaa cttcctgtaa gcgctcttcc tccagatggg acttacccaa
     9841 ctggaactac aaaatgggaa aaacgcaatc ttgcgcttga aattcccgtt tgggagcctg
     9901 agatctgtat acagtgcggg aaatgcacta ttgtatgccc acacgccgta attcggatta
     9961 aggcctatga tcctaaatat ctcgaagggg ctccagacaa atttaagtca tcttatgctc
    10021 gcgatgaagt atggaaagga cttaaataca ctatccaagt agcgcctgag gattgtaccg
    10081 gatgcggaat gtgtgtagat atctgtccgg ctagaagtaa atctgaaact agaattaaag
    10141 cactcaacat gtatccgcag cctcctttac gggaggagga aagtaagaat tgggatttct
    10201 ttttaaccat ccccgagacg gatcgaatcc atctgcaccc aatgagtgtg cgtcatcagc
    10261 aggtacagca accactattc gaattctcaa gcgcttgtgc cgggtgtgga gaaacaccct
    10321 atattaaact acttagccag ttattcggag atcgtttgat agtggctaat gctacaggct
    10381 gttcttctat atatggtggt aatcttccca ccacaccatg gactacaaac gctgagggtc
    10441 gcggaccggc atggtctaac tctctctttg aagacaatgc cgaatttgga cttggttttc
    10501 gactttcgat tgacatccaa agagagtttg ccgccaattt gctcaaaaag gtgtcgccgg
    10561 ttattggtga aaacctagcg catgcaatcc taaatgcgga gcaaaaagat gaagcggata
    10621 tttacgaaca gcgtaagcgt gtagaagctt tgaagaagtg gctccaggat gttgagtccc
    10681 aagatgcaag acggctccta agcattgctg atatgctggt caaaaagagt gtttggatta
    10741 tcggtggaga cggttgggca tatgatatcg gttttggcgg ccttgaccat gtactggcta
    10801 gtgggcacaa cattaatatt ttggtacttg atacagaggt ttactctaat actggcgggc
    10861 aaatgtccaa gggaacccct cttgccgcag tagctaaatt tgcctctgcc ggaaagccca
    10921 cccccaaaaa ggatcttgga ctaattgcca tgacttacgg cgatgtttac gtagcaagtg
    10981 ttgcaatggg agcaaaggat gaacacacaa ttagagcttt ccttgaagca gaggcctatg
    11041 agggaccgtc acttatcatt gcctacagtc actgcattgc acacggaatc aacatgacta
    11101 cagctatgcg aaatcaaaaa gccgctgtag attccggaca atggctactc tacagattca
    11161 atcctgaacg ggcaaaacaa ggagaaaatc cacttcacct cgattcacaa gcaccgaaaa
    11221 taccagtaca gcagtacctg tatatggaaa accgattcaa aatgctcctc aagactcgac
    11281 caatagaggc caaacgctta ttcaaggagg ctcaacagga aataaattcc cgctggagcc
    11341 tttatgaata tctggccgca cgcacgaaaa tcggaaatcg agaagagaaa tcaaagtagt
    11401 ggttagtaac actacgtacg ggtgtcgcag aaagataatt ccataagtaa tcatgagagg
    11461 aggacagatt aatggatttg agtacaacat atttagggat gaccttgcgt acgccattgg
    11521 taccatcggc atctcctcta tctgaagaga ttgacaatat caaacggatg gaggatgctg
    11581 gtgcatcagc cataatactt tattcgttat ttgaagagca actacaacta gagcgctacg
    11641 aacttcatca tcaccttact catcacactg atagctttgc agaggcactt accttctttc
    11701 cggaaccaac agagttccat gtcggaccag aaagttactt gtatcatata caaaaggcca
    11761 aagaagcggt gagtattcct atcattgcta gtcttaacgg cacttcagct ggagggtgga
    11821 tagactattc aaaaaagata gaacaggcgg gggcagatgc acttgaactt aacatatact
    11881 atattcctac tgatatggaa ctaacctctg agcaggtcga gcagacctat gttgagattc
    11941 tcaaggcggt gaaatccgag gttaaaatcc cggtggcgct taaactgagc cccttcttca
    12001 ataacatagc ctatatggcc aaacgccttg acgacgcggg ggcaaatgct ctagttctat
    12061 ttaatcgttt ttatcaacct gatattgacc tcgaaaatct agaggttcaa ccaaacatta
    12121 ttctgagttc accccaggcc ttgcgactcc ctcttagatg gattgctatt ctctatggtc
    12181 gcgttaaggc agatctagct gcgacaagtg gcattcatac agcacgggat gtaataaaaa
    12241 tgctcatggt cggtgctaat attaccatgc tatgctccgt acttctacga catggtatag
    12301 aacatattcg tgttatagaa caaggagtaa gtaattggat ggaagagcat gaatatgaat
    12361 ccgtacggca gatgcaggga agcatgagcc ataagaattg tgaggaccct tccgcattcg
    12421 aacgctctca gtatatgcgt ggtctcctta cctataaacc aaaagcataa ctatacggct
    12481 ctgatgctca ccgaggggcc ccggaaatga accaagtaga ggctttacgt catcatgact
    12541 agttcaaatt aatatggata agttttaaga gaattttctt tgatgaagag gtattattgt
    12601 tatataaact ctttatgagt aaataaatat aaaggtcatt ttagtgtaat aaatagaaaa
    12661 cgctatatat attatgatag agccattttt acgactcgag aataaatccg ttttagagcg
    12721 ccaaaacctt gcggatggga gacttcatgg acttgaagac ccgaagagcc cgatgggaga
    12781 acatatactg attgattact ttgccagttc atgttgggat tacgtatggc atgaagctat
    12841 ggagaaagcg cattacataa ggaggcacgg accgtttatg cctccaacac ttcccccgga
    12901 ggagatgggg tatacaacca aaggtcgaag ttcttaagaa actcaagcct cgaatggaag
    12961 agatagatta agactgatcg cagtaattac tccctactca ttagtgagtt tcttcgtcct
    13021 ttaccgcttc tctttaaggt cctcagggat ggtttcttat tttaaaatgt tttaaataaa
    13081 tcctagaact taaattaaac cagatatatt atcttggttg attatcacat gaaaagaaat
    13141 atttttgtta gatccctaac tggaaggtcg cctcatgaag gggatgttga aattgttgaa
    13201 agaaaaggtt tagggcatcc tgacacgatc tgtgataacg tcgctgaaga ggtctctatt
    13261 gctctaagca aatattattt agatgagttt ggggcagtga tgcatcacaa cgttgataaa
    13321 gggcttcttg ttggagggat ttcaaatcct tcttataatg gcggagaaat agttgaaccg
    13381 atagagctga caattgtggg aagggcaata aaagaaaaaa gagaaagaaa tctacccgtt
    13441 gaagaaatag ctatagaggc tatgcataaa tggttaaaag aaaacataag gcatcttgat
    13501 actgaaaatc aaataattac aaaggtaaag ataagacccg gaagcaaggg cttgattgat
    13561 ctttttgaaa gatttcgtga gggaaaggta cctttatcaa atgatacttc aattggaaca
    13621 ggtttttatc cattagatga acttgagaaa accgtttata aggtagagag attgcttaac
    13681 ggagctgaaa taaagagaaa ataccccttt ataggagagg acataaaggt tatagccata
    13741 agaaatagag agaagataag gcttacagtt gcgatggcaa ctgtagacaa atacatactg
    13801 agtttgaatg attacattga caagataagc atggttaaga agcttttggt taaagagatt
    13861 tcccttggct caaatctaga ggttgaaata aatacagcgg ataagtatga agatgagagt
    13921 atatatctaa cagttactgg cactagcgga gaacacgggg atgacggtca ggtcggaagg
    13981 ggaaataggg ttaacggttt gataacacct tatagaccca tgagtttaga agccgctgcc
    14041 ggtaagaatc ctataagcca catcggcaaa atttacaatc tgtttgctaa tgatttgagt
    14101 aaggcaatag ttgagacggg tcttgcagat gaagcttatg tttatatcgc atctgagata
    14161 ggaaagccaa taaatcaacc tcaggtcctg gatatagggg ttaaaggtaa gaaaacagat
    14221 aacgaagcta taacaactat agcagaggaa atgctcgatg aaatgcctaa tttatggaaa
    14281 aaggttatcg agggcaaata tcagattgca tgatgaaaaa aggagcccat ctatattcta
    14341 gtctagagcc ttacgatgaa tattacgagg tttcgaccga atataaaaag agagaaaata
    14401 tttattaatg ttagatccca taatacatca ataattttag atacgtttac ggttcccaca
    14461 ttcgacaaag aaggttaatt gttaattcca attgattgta cgttgaagat tcagctaagt
    14521 taatagcaca atccgaagcg cccattaaat ttaaatttgg gatcatacct gatgaatgga
    14581 acttctacat agagctctat taacccaaaa tgcgaaatgg aataaggaaa agaaaagtgg
    14641 taataatggg agctgctggg agggattttc ataacttcaa tatcttcttt agaaataaaa
    14701 aggattatga ggtcatagca ttcacagcag cacagatacc caatatagaa aagagaatat
    14761 atccgcctga gctatccgga ggactttatc cgaaaggcat ccccatatat ccagagtccg
    14821 ggataaaaga aatcctagag aagaataagg tagatgaggc aatccttgcc tacagcgacc
    14881 tatcgaatga agaggtaatg gagaaagcca gtattgtgct ctcttccggg gcggatttta
    14941 gactcatggg accctatagt accatgctga aatccaaaaa gcccgtaatt tctgtttgtg
    15001 ctgtgagaac tggggcagga aaaggtgtaa caaccagaaa aataattaga tttcttaggg
    15061 aaaaaggtta taaaactgtg gtagtaagac accctatgcc ttatggtaat ttgaaaaagc
    15121 agatatgtca gaggttttcc aaatacgagg acttagaaaa aaacgattgc ac
//
LOCUS       DATDRL010000304        11302 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_902528, whole genome shotgun sequence.
ACCESSION   DATDRL010000304 DATDRL010000000
VERSION     DATDRL010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11302
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902528"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1860)
                     /locus_tag="VI935_10220"
     CDS             complement(<1..1860)
                     /locus_tag="VI935_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HLE26010.1"
                     /translation="MELHQIEEKLRWYEAREAILKRISPELNSVIDIDKFLIATVQEL
                     GKMMGVDKCDFIVPGDQSLRVEYEYRKDGSVPSTLNLRIQFRENGWEASSIGTIVVND
                     AAQDKFDERLKEILTRNGTRSFLCIPVVFQDELLGILGFHHCKEVHKWSDDEINFLKS
                     LTQQTSIALQYTRLYTRMEKEVEITKTLLEITNEINSKVDFSEITSFVLRKAVELLDA
                     TYGSIGILDTAERKLSITTFYPEATPSLRNISLNVSLNAPYYDAILDKKTVGIFGEES
                     PGDSRQLMNALRGRSAIINPIVIDNRTYGTLNLVWDVKKDIFKPHEVELVSGITSQMA
                     VVLEKNRLSSELILLKRELTGVSASEMIVGVSKKIEKCVETALSVIDSDVTILVQGET
                     GTGKELIADLIQKNSNRKDKPYIKINCGAIPETLIEAELFGYEKGAFTDAKVQKIGKF
                     EIANGGTIFLDEVGELSLNAQVKLLRVLQNGELFRIGGNQPITVHVRVIAATNIDLEE
                     AIRNQRFRSDLFYRLNVFPIVIPPLRERKEDIPLLAHHFLEIYKKKSKKLIIGISDDA
                     MKLLKTYDWPGNIRELENITERAVITCTRKVVTVDDLPKNIIDSVKKETDSRIV"
     gene            2184..3965
                     /locus_tag="VI935_10225"
     CDS             2184..3965
                     /locus_tag="VI935_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="activator of (R)-2-hydroxyglutaryl-CoA
                     dehydratase"
                     /protein_id="HLE26011.1"
                     /translation="MDNNVDRISDGRDGNAKEDFVRIESFIHRRLQEEKARLEKELGI
                     EEKPAHHFYKPIERPFTRSQRPYTTVLYGGLTWKHEHLVHGALEGLGYKVEYIPTADK
                     AAFQLGKEYGNNGQCNPTYFTVGNLVQYLQKLESKGISRQEIIDKYIFVTAGACGPCR
                     FGMYEAEYRLALRNSGFDGFRVILFQQTGGLNQADEGAGLEMNMDFFFGMLNGMNMGD
                     LISDVVYQIRPYEVNKGETDKVLGESMDYLHEVLKKHKPFKLEGKIGEFLKKFPKVAY
                     SSEYIGKFLTQIYGDEYTAALKEVGDRFNSIEVDRTRIKPIVKVTGEFWAQTTEGDGN
                     FNMFNFLEREGAQVLVEPIGTWIMYMIHQAKQAINDKRGLPKNGDHVPGWWRLDQALS
                     KYKDHQKKIGMLTAAEVIFKREWNRMRESLGNLPHDLTDQYELQRLGHPFYNSRAGGG
                     EGHLEVAKNIYYSNKDLAHMVLSLKPFGCMPSTQSDGAQSAVVNLYKEMIYIPIETSG
                     EGDVNAHSRVQMALGEAKVKAKNEFQEALSKTGYTLDEIKNYVEENPELKQPFYHIPH
                     YKGMVGVAAGFVRHVGECMKIGKRHAA"
     gene            4036..7551
                     /locus_tag="VI935_10230"
     CDS             4036..7551
                     /locus_tag="VI935_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003390374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HLE26012.1"
                     /translation="MGQSIHEPLFIGMDVGSTTVKAVVVDIVNDEILWKDYQRHDTKQ
                     PEKTLELLKRIEGDLGLPSDKFRVFITGSGGGGITRLIGAKFVQEVNAVSLAVEKLHP
                     EVGSVVELGGQDAKIIIFKEDPETGKKKKIPSMNDKCAGGTGAVIDKINAKLRIPAEQ
                     LCNMGYYDLKLHPVAGKCGVFAETDINSLQKMGVPPDELMASLFEAIVGQNLAVLTRG
                     NTLRPVVLLLGGPNTYIRGMLEAWRYNIPKIWEERSVQLPEGVDPKELIKVPQNAQYF
                     AALGAVEFGKQEDSNVGVYKGTKDLEYYIGFGRAEEKKKKGAQGLSKNAEELETFKER
                     YKRKPFIPATFKPGELIEGFIGIDGGSTSTKAVLMNKNRELLAKAYQLSKGNPIEDTI
                     EMFVKLRAKVEEQGATLEVLGVGTTGYAKDILKDVLRADVALVETVAHTEAGLHFYKE
                     IDVICDVGGQDIKIMILKNRKVKDFKLNTQCSAGNGYFLQGTAEGFGIKVEDFADIAF
                     GAEAMPTFGYGCAVFMQSDIVDFQRQGWKPEEIMAGLAGVLPKNIWLYVSQIPNLAKL
                     GTNFLLQGGTQNNLAAVKSQVDFIESRFKGSDHKPRVIVHEHCGESGAIGAAIEAVRL
                     WENGRRTEFVGLDAVEQITYTTHRSEDTRCYFCKNKCLRTFIDVKIAGNTENTISPKS
                     KIQAGAGIKRLIVGNSCEKGLVEDIEDMKVIKKGLDSAKKANPNLVETAAKAVWRSFN
                     PENVSDPLPKYTFTKAQKKRAELMKKRKNLRIGIPRVLNMYSQNPVFSAYFESLGIPS
                     ENLVYSDFTTEELYKEGAKRGAIDPCFPSKVGIPHVHNLVYHHHKKKPLNIIFFPMID
                     DLTSDLVNTWDSRSCPTVATTPAAVKAAFVKEGDIFAQNGIIFLDTFVNINDCPLFER
                     QMYDQFNDLLGLSEEENRRAVEQGYKALDKFNNDVQRGAGREVLSKLEAEDKLGVVLL
                     GRPYHNDPGINHEILEEFQKLGYPVFAEDALPIDDDILERLFGEDVRKGIISHPMDIS
                     DVWKNSYSENTSRKVWAAKYIARHPNLVALELSSFKCGHDAPIYTVVEEIVEDSGTPY
                     FCFKDIDENKPTGSIKIRVQTIDYFLRRYREDMVKNKEKKAKLQERLREIEAKLREEL
                     SSSTYEGNIGGGFFDDQRVAFKNKE"
     gene            7663..7944
                     /locus_tag="VI935_10235"
     CDS             7663..7944
                     /locus_tag="VI935_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26013.1"
                     /translation="MPVESDKIMFMIYRDTTYTDKYRVCYFTELDEHNKESEINKALS
                     GEPFLDGFIKNYKKDRAKEIIAKFLERLNNGEYVDPSEISKALDEFIPK"
     gene            8091..8366
                     /locus_tag="VI935_10240"
     CDS             8091..8366
                     /locus_tag="VI935_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HLE26014.1"
                     /translation="MLCLHNYKNGTLYVGLTNNLIRRAYEHKNDLIEGFTKKYGVHKL
                     VYYKQTNDIHNAIQREKELKKWNRQGKINLIEKFNPNWNDIYDELTK"
     gene            8385..8852
                     /locus_tag="VI935_10245"
     CDS             8385..8852
                     /locus_tag="VI935_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26015.1"
                     /translation="MEKLILYIISVISLILAAYCLIRVFKLKEEVQRTRDEADALRKH
                     VGYSLDQLKTDIIAGLKTEMRKQSGAPYFHKDMTIGDALKLHPLAQTVMAGFHLGGCS
                     SCDIKEDHILGAAAKDYGIELDNLLEALNGLLDGTTVLPSEQVHQIKLVDMLK"
     gene            8907..9140
                     /locus_tag="VI935_10250"
     CDS             8907..9140
                     /locus_tag="VI935_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016515172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26016.1"
                     /translation="MKFEIVGKIEDIEIIATGKNIRDLSYLQKIYGRGRWRKLKGIAQ
                     VKLPNGRIRRAEIHWYEAHGIGRKDLKIKYYLE"
     gene            9144..9368
                     /locus_tag="VI935_10255"
     CDS             9144..9368
                     /locus_tag="VI935_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26017.1"
                     /translation="MSKSEKRFALCIDNTDYKASLIPRKLYEIIPDEDAAKDDFIRII
                     DESGEDYLYHKSLFIIFELPAEVEQAMSRA"
     gene            10270..10932
                     /locus_tag="VI935_10260"
     CDS             10270..10932
                     /locus_tag="VI935_10260"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HLE26018.1"
                     /translation="METKRVFDRFRFIANLAEKEFDRNFGLYPGQMQCRKGCFLCCTQ
                     MFRITLIDAAIVSKAVKKLDPEILKKMQDKARLYLQKKDEMVHERAEEMNIDHESVEI
                     STIGLRLPCPALDENGACGIYESRPIVCRKFGMPIYDSKKPEELQTCELNFHEVKKID
                     AEPLIENQMKIYNYWMELKTEVNETLNPDKRATTIAEAILFDYEEMLELNLQKPNPLC
                     GS"
     gene            10981..>11302
                     /locus_tag="VI935_10265"
     CDS             10981..>11302
                     /locus_tag="VI935_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE26019.1"
                     /translation="MTLVQIKNIFKEKLKLIEMIFRFIRRKDDNSFIRGLSEFALNYD
                     VDAEIETQKRFLEENPNNAKGYFNLGVLYYFQGKVEDAINSYTKALEVDPNLSEAHKN
                     LGEIY"
BASE COUNT         3537 a         2234 c         2492 g         3039 t
ORIGIN      
        1 tacaatccta ctgtccgttt cctttttaac agagtctatt atgtttttag gcaaatcgtc
       61 tactgtaaca acctttctag tacaggtaat gacagcacgc tccgttatgt tttcaagctc
      121 acgaatattt ccaggccaat cataggtttt aagaagtttc atcgcatcat cggatatacc
      181 gatgattaat ttcttcgatt tcttcttata gatctcaaga aagtggtggg caagaagcgg
      241 aatatcctcc tttcgttcac gaaggggggg gataactatt gggaaaacat taagcctgta
      301 gaataggtcg gaacgaaatc tctgatttct tattgcctcc tcaaggtcaa tgttcgtcgc
      361 cgcaattact ctaacatgca ctgttattgg ttgattgccg ccgattctaa aaagctcacc
      421 attttgtaaa accctaagga gtttcacctg ggcattgagg ctaagctctc ctacttcatc
      481 aagaaatatg gtgccaccat ttgcaatctc aaatttacct atcttttgta cctttgcgtc
      541 ggtaaacgca cccttttcat aaccaaacag ctctgcttcg ataagggttt cgggaattgc
      601 accacagttt atttttatat atggtttgtc ttttcgatta ctatttttct gtattaaatc
      661 ggctatcaac tctttaccag ttcccgtttc accttgaaca agtattgtta catcactatc
      721 aataacactt agcgccgtct ccacgcactt ttcaatcttt ttactcacgc cgacgatcat
      781 ttcgcttgcg cttactccgg taagttctct ttttagtaat atgagctcag aagaaagtct
      841 attcttctca agcactacag ccatctggct cgtgattcca cttacaagtt caacttcgtg
      901 aggtttgaat atatccttct tgacatccca cacaagatta agtgtcccat atgttctatt
      961 gtcaattaca attggattta taattgcaga cctgcctctt agtgcattca taagttgtct
     1021 tgaatcccct ggtgattcct ccccgaatat acctaccgtt tttttatcta atatcgcatc
     1081 ataatatggc gcatttagag atacatttag acttatattc ctaagcgagg gtgtagcttc
     1141 tggatagaat gttgtgatac ttagctttct ttcggcagta tcgagaatac caattgagcc
     1201 gtaagtagca tctaataact caacagcctt tctgagaacg aatgaagtta tttccgaaaa
     1261 gtctactttt gagtttatct cgtttgttat ctcaagaagg gtttttgtga tttccacctc
     1321 cttttccata cgagtataaa gccttgtata ttgaagcgct atggatgtct gctgagtaag
     1381 ggattttaag aaatttatct cgtcatctga ccatttgtga acctccttac agtggtgaaa
     1441 tcctagaatc cctagaagct catcctgaaa aaccacagga atacaaagaa aagagcgagt
     1501 gccatttctg gtcaggattt ctttaagcct ctcgtcgaat ttatcttgag cagcatcgtt
     1561 aacaacaata gttccgattg aacttgcttc ccatccattc tcgcgaaact ggattcttaa
     1621 attcaaggtt gaaggaactg aaccatcctt cctatattcg tattccactc taagactttg
     1681 atcgccagga actataaaat cgcatttatc tacacccatc atctttccta gctcttgcac
     1741 agtggcaata aggaacttgt ctatatctat aacactgttt agctcaggac tgatcctttt
     1801 aagaatcgct tccctagctt cgtaccatct caatttttct tctatttgat gtagttccat
     1861 tgcttcacct ctcatctaaa gatagccact aagccaccaa ggcacgaaga aaaaattctt
     1921 tgtgtcttag tgccttggtg gctaataata ccctatttag agacaaaagt atacacagaa
     1981 tacacaaaaa ttctctctat gtatcctttt atcctatgaa ataacattaa agtgtatcct
     2041 ttttatacac aatagtaaag aacgtgatat ttagattcta ataatgctaa aattattcca
     2101 tgttttcaat aggttagaaa caagcacagc ctctggcatt attcttgcat aatattaact
     2161 caaaccccat aaaggaggta gttatggata ataatgttga tagaatttca gatggtaggg
     2221 atggcaatgc taaagaggac ttcgtaagga ttgaaagctt tattcaccgt agacttcagg
     2281 aggaaaaggc cagacttgag aaggagctag gtatagaaga aaaaccggct caccattttt
     2341 ataaacctat tgagcgtcct tttaccagat cacagagacc atatacaaca gttctttatg
     2401 gggggctaac ctggaaacat gagcatcttg ttcacggtgc gttagagggc cttggataca
     2461 aagtcgaata tattcccact gcagataaag cagcattcca gttaggaaag gaatatggaa
     2521 acaatggtca gtgtaaccct acatatttca cagttggaaa ccttgtgcag taccttcaga
     2581 aactagaatc gaagggaatt tctaggcaag agattattga caagtacata tttgttacag
     2641 caggggcttg tggaccatgc aggtttggaa tgtatgaagc tgaatatcgc cttgctctta
     2701 ggaattcagg ttttgatggc tttagggtaa tccttttcca gcagaccggt ggcctcaacc
     2761 aggcagatga aggcgcaggg cttgagatga acatggattt cttctttggg atgctaaacg
     2821 ggatgaacat gggtgattta atcagcgatg tggtttatca gataagaccc tatgaggtga
     2881 ataaaggaga aactgataaa gtccttggag aaagcatgga ctatctgcat gaggtgttaa
     2941 aaaagcataa gccgttcaaa ctcgaaggca aaattggaga atttttaaag aagttcccaa
     3001 aggttgccta ctcatccgag tacatcggca agttccttac ccagatttat ggcgatgaat
     3061 acaccgctgc cttaaaggaa gttggagaca ggtttaacag catcgaggtt gaccgcaccc
     3121 gtataaagcc gattgttaaa gtcacgggtg aattctgggc gcagaccacg gagggtgatg
     3181 gaaacttcaa tatgtttaat ttccttgaga gagagggggc ccaggtgcta gtagaaccaa
     3241 ttggaacatg gattatgtat atgatacatc aggcaaagca ggctataaac gataagaggg
     3301 gtcttcctaa aaacggcgat catgtaccgg gctggtggag gctcgatcag gctttatcta
     3361 aatacaaaga ccatcaaaag aagatcggaa tgcttactgc cgccgaggta atattcaaac
     3421 gcgaatggaa tcggatgaga gaatctttag gcaaccttcc tcacgacctc acagaccagt
     3481 atgaactcca gaggcttgga catccgttct ataattcaag agcaggcggc ggagaaggac
     3541 accttgaggt agcaaagaat atttattaca gcaataaaga ccttgcccac atggttctct
     3601 cactaaaacc gtttggctgt atgccgagca ctcaaagcga cggcgctcag tccgcagtcg
     3661 tgaatctata taaggaaatg atttacatac cgattgagac atccggtgag ggagatgtaa
     3721 acgcacacag ccgtgttcag atggctcttg gcgaggcaaa ggtaaaggca aagaatgaat
     3781 tccaagaggc actttctaag acaggttata cattagacga gataaaaaac tatgtggaag
     3841 aaaatccaga gttaaagcag cccttctatc acattccaca ctataaagga atggtaggag
     3901 tagcagcggg atttgtccgt catgtaggag aatgcatgaa gataggaaaa cgtcatgcgg
     3961 cataaacagg atgcaggata caagatgcaa gatggatcat gaatcatgat tataaattct
     4021 ctggaggcat aataaatggg acaatcaatt catgaaccgc tcttcattgg catggatgtg
     4081 ggttcaacta ctgtgaaagc cgtcgttgta gatatagtta atgacgaaat tctctggaag
     4141 gattaccaaa ggcatgacac aaagcaacct gaaaagactc tggagctttt aaaaagaatc
     4201 gaaggcgacc ttggacttcc atccgataaa ttcagagtat ttattacagg aagcggtggt
     4261 ggaggaatta cccgtctaat tggggctaag tttgtccaag aggttaatgc cgtttctctt
     4321 gccgttgaaa agctccatcc tgaggtcggc tctgtagtcg aactcggcgg acaggatgca
     4381 aaaataatta ttttcaaaga agatccggaa acaggaaaga agaaaaagat tccaagcatg
     4441 aatgacaaat gcgccggcgg tactggcgct gttattgata agatcaatgc aaagctcaga
     4501 atcccggctg agcagctctg caatatggga tattacgatt taaaactgca ccccgttgca
     4561 ggaaagtgcg gtgtctttgc agagactgat ataaacagcc tccaaaagat gggagttcct
     4621 ccagatgaac tcatggcttc gctttttgag gctatagtag gacagaatct tgcagttctt
     4681 acccgcggga acacactccg ccctgtagtt cttctcctag gcggtcctaa tacatacatc
     4741 aggggcatgt tagaagcatg gagatacaac atccctaaga tttgggaaga aagaagtgtt
     4801 cagcttcctg aaggagttga tccaaaggag ttaatcaagg tcccccaaaa cgcccaatat
     4861 tttgctgcac ttggagccgt tgagtttgga aaacaagaag actccaatgt tggagtatat
     4921 aagggcacca aagaccttga atattacatc ggctttggca gagccgaaga aaagaaaaag
     4981 aaaggggctc aggggctaag taaaaatgca gaagaacttg agacatttaa agagagatat
     5041 aaaaggaaac cctttattcc tgcgactttt aaaccaggtg aactaatcga aggcttcata
     5101 gggattgacg gggggtcaac ctcaaccaaa gcggttttaa tgaacaaaaa tagagagcta
     5161 ctggctaaag cctatcagtt atccaagggg aacccgattg aagacactat agagatgttt
     5221 gtaaagctgc gcgctaaagt agaagagcag ggagcaacgc ttgaggtgct tggcgtagga
     5281 acaaccggct acgcaaaaga tattctcaaa gacgtcctta gagccgatgt tgcccttgtt
     5341 gagaccgttg cccacacaga ggctggtctt cacttctata aagaaattga cgttatatgc
     5401 gacgttggcg ggcaagacat caagataatg atattgaaaa acagaaaggt taaagatttt
     5461 aaacttaata cccagtgttc tgctggaaat gggtatttcc ttcagggaac ggcggagggc
     5521 tttggcatca aggttgagga ttttgcagat attgcttttg gcgctgaagc gatgcctact
     5581 ttcggctacg gatgcgctgt ttttatgcag tctgacatcg ttgacttcca gcgccaggga
     5641 tggaagcccg aagaaatcat ggcagggctt gccggggtgc ttcctaagaa catatggctc
     5701 tatgtttctc agattccaaa ccttgcaaag cttggaacga acttcctcct tcagggtgga
     5761 actcaaaaca acctcgcagc ggtaaaatcc caggttgact ttattgaatc gaggtttaaa
     5821 ggctcggatc acaaaccgag ggtcattgtc cacgagcact gcggggaaag cggggcaatc
     5881 ggcgctgcaa tcgaagctgt tcgcctctgg gaaaatggaa ggagaacaga atttgtcggg
     5941 cttgatgccg tcgaacagat cacatatacc acccacagaa gcgaggatac cagatgttat
     6001 ttctgcaaaa acaagtgtct tagaacgttt attgacgtta agattgccgg aaatacagaa
     6061 aacactatta gcccgaaatc caaaatacag gcaggagcgg gcattaagcg tcttatcgtt
     6121 ggaaatagct gtgaaaaggg gttggtagaa gatattgagg atatgaaggt tataaagaaa
     6181 gggctggaca gtgcaaagaa agcaaatcca aacctggtcg agacggcggc aaaagccgtt
     6241 tggaggtcat tcaacccaga aaacgtttcc gatccgcttc cgaaatacac cttcacaaag
     6301 gctcagaaaa agcgcgcgga gcttatgaaa aaaagaaaga accttagaat cggaatccca
     6361 agggttctaa atatgtattc tcagaaccca gtgttcagtg catactttga gagtctcgga
     6421 ataccttctg agaaccttgt ctactccgat ttcaccacag aggaacttta caaggaaggt
     6481 gcaaaacgtg gagcaattga cccatgtttc ccgagcaaag tcggaatacc ccacgttcat
     6541 aatctcgttt atcatcacca taaaaagaaa ccgcttaaca tcatcttctt ccctatgatt
     6601 gacgacttaa catccgacct cgtaaacaca tgggatagca ggtcttgtcc aactgtagca
     6661 actacaccgg cagccgttaa agcggccttt gttaaagaag gtgatatatt tgctcaaaat
     6721 gggataatct tccttgatac ctttgttaat attaatgatt gccctctttt tgaaaggcag
     6781 atgtacgatc agtttaatga tctattagga ctttctgaag aagaaaacag acgcgccgtt
     6841 gagcaaggat ataaagcact agacaagttt aataacgacg ttcaacgtgg agccggacgc
     6901 gaggtgctga gcaaactcga agctgaagat aagctagggg tcgttcttct cggacgtccg
     6961 tatcataacg accccgggat taatcatgaa atcttagaag agttccagaa gcttggttat
     7021 cctgtttttg ctgaggatgc cctcccgatt gatgatgata tacttgaaag gcttttcggc
     7081 gaggatgtta gaaagggaat aatctcacat ccaatggata tatcagatgt gtggaaaaac
     7141 tcatacagcg aaaacacaag ccgtaaggtc tgggctgcta agtatatcgc aaggcatcca
     7201 aacctcgtgg cattagagct ttcaagcttc aaatgcggcc atgatgcccc aatttatacc
     7261 gtggttgagg aaattgttga agactccggc accccatact tctgcttcaa ggatattgat
     7321 gaaaataaac ctaccggttc aataaagatc agggttcaga caattgacta cttcctccgc
     7381 aggtacagag aagacatggt taagaacaaa gagaaaaagg ctaagcttca agaaagactc
     7441 agggaaatcg aagcgaagct cagagaagag ctctcttcaa gtacatatga aggaaatatt
     7501 ggcggcggct tttttgatga ccaaagggtt gcctttaaga ataaagaatg aaaccaacta
     7561 ggggcaattc ataaattgcc cctcctccgt aatgataaac ccctttctga atagattatt
     7621 atctgataga attggtatta gttagaaaga ggtgtttgca agttgccggt cgagtcggac
     7681 aaaataatgt ttatgattta tcgtgataca acctatacag ataagtatag ggtttgttac
     7741 tttaccgagc ttgacgagca taataaagaa agcgaaataa ataaagccct gtcaggagaa
     7801 ccttttcttg atggttttat taaaaattac aaaaaggata gagcaaaaga aatcatagct
     7861 aagtttcttg aaagactaaa caacggcgaa tatgttgacc caagcgaaat ctcaaaagct
     7921 ttagatgaat ttattccgaa ataactattt atctactaag acacgaagta tgttggagta
     7981 gtaacgaagg ttgtcattcc cgcacatttt tagcgggaat ccagtattta aaacgtttat
     8041 ggatgcccga ttaaaacatt cgggcatgac aatagtaaag ttgtgaatac atattatgtt
     8101 tacataatta caagaatggt accttgtatg tcggtcttac taacaacctt attaggagag
     8161 cttatgaaca caagaatgat cttattgaag gatttactaa gaaatatggt gtacacaaat
     8221 tggtctatta taaacagact aatgatatac acaacgcaat ccagagagaa aaagagctaa
     8281 agaagtggaa ccgacaaggg aaaattaatc ttattgaaaa attcaatcct aattggaatg
     8341 atatatatga tgagttaaca aaataattaa ggagaccaat ttaaatggaa aaactaattc
     8401 tctacataat ttcagttatc tctctaattc ttgctgcgta ctgtctaatt cgcgtattca
     8461 aacttaaaga ggaggttcaa cgcacccgtg atgaagcaga tgctttaaga aaacatgtag
     8521 gctattccct cgaccaacta aagacagaca taattgcagg tttaaaaacc gagatgagaa
     8581 aacaatcagg tgccccctat tttcataagg atatgacaat cggcgatgcg ctgaaactcc
     8641 atccacttgc tcagaccgta atggctgggt ttcatcttgg cgggtgttca agttgtgata
     8701 ttaaggaaga ccatatactg ggagctgccg caaaggacta tggaatagag ctcgataatc
     8761 ttctcgaagc gctaaacggc ctccttgatg gaaccactgt ccttccttcc gaacaggttc
     8821 atcaaattaa gcttgtagac atgctaaagt agaagagact aacgcttctt aagcctggag
     8881 taccgaatgt atgcaaaact ataactgtga aatttgagat tgttgggaag attgaagata
     8941 ttgaaattat agccacagga aaaaacattc gtgatctaag ttacttacaa aagatttatg
     9001 gtcgagggag gtggagaaag ctgaaaggca tagcgcaagt caagcttccc aacggcagaa
     9061 ttcgacgtgc tgaaatacac tggtatgaag ctcacggtat cggtcgtaaa gatttaaaaa
     9121 tcaagtatta tttagagtaa gctatgagca agtcagaaaa gcgtttcgca ttgtgcattg
     9181 ataataccga ttacaaggcg tcccttattc caaggaagct ctatgagatc attcctgatg
     9241 aagatgcagc caaagacgac tttattcgca taatagatga aagcggtgaa gattatcttt
     9301 atcacaaaag cctgtttatt atttttgagc ttccagcaga ggtagagcaa gcgatgagta
     9361 gggcttagtg tcaagtccct gtccgcctag gactgttgcg gcttgtaatt agcttgttgt
     9421 gcgcgtatgg cgaaggggtg aggctttata cttttgagcc cctgaggtaa agaagcgact
     9481 ttgatacttt cctttggtaa gttcttcgaa ccatttacac acttgatgac tacgcctgtt
     9541 aatttagaga agttctttaa tatgttcttt taatactgat aagtggtgca aagatttccg
     9601 tgtaacatcc cgtttagggc ggttatacgg aaaagattcc gtataatcaa tagttagccg
     9661 caacacatga acaccgtact gataaccgct cttcgaagac gcaccgcaag tatctcggtc
     9721 ggtccctcaa ctgcgagaaa tatggggccg agaggaactg ttggcgctgc ccgtgattat
     9781 ctagcgggcc ttgacctcag gaaattcagc aagaaaacgg agaaggaatt tcttgcatcc
     9841 ttaaatgctg ccaccgttgc cttcgtgaag catcttcctt tgggtgcaaa gcggtggggc
     9901 tccgcgagaa aattcttgaa catattcttg cgtgaggtga tatacaaccg gttcctttgt
     9961 gaacactata acctttaccg cattgaaccg tggctcgagt tgccgcttga cagtcatgtt
    10021 gctgagggac tccgggaaga gtctggtggg cattcccttc ctcggtggaa gacagttatc
    10081 ggcttagatt ccgagactaa tcaccagtac caagaacaag cgaccaaagt agcgaaaaca
    10141 aagaacatac atcgtgtcca tctcgatgtt ctgtattggc gtcgtgaatt tgttgcgcct
    10201 aacacgtcgc cccaccggac gcggaatacg aggccgcgcc agtgaactct gcgttaggga
    10261 gttaagcttt tggaaaccaa aagagtgttt gatcgcttta gatttatagc caacctagca
    10321 gaaaaggaat ttgaccgcaa ttttgggctc tatcccggtc agatgcagtg tagaaaaggg
    10381 tgttttctat gctgcactca gatgttcaga attacactta ttgatgcagc cattgtttca
    10441 aaagctgtaa agaaactcga tcccgaaatc cttaaaaaga tgcaggacaa agcaagactc
    10501 taccttcaga aaaaagatga aatggttcac gaaagagccg aggagatgaa catagatcac
    10561 gaaagtgtag aaatctccac aatcggctta agactcccct gccctgctct tgatgaaaac
    10621 ggtgcctgtg gaatttatga atctagacct atagtctgcc gcaaattcgg gatgcctata
    10681 tatgattcaa aaaagccaga agagcttcaa acctgcgagt taaacttcca tgaagtaaag
    10741 aagattgatg ctgagccgtt aattgaaaac caaatgaaga tttacaacta ctggatggaa
    10801 ctaaaaaccg aagtgaacga aactctaaac ccagataaga gggccacaac tattgctgag
    10861 gcgattttat ttgattacga agagatgctt gaacttaatt tacagaagcc aaacccatta
    10921 tgtggctcgt gactggtagt ttgatattcg atccacgaat cacgatccac gaatcacgat
    10981 atgactctcg ttcaaattaa gaacatattt aaggaaaaac tgaagctcat agaaatgatt
    11041 tttagattca ttaggagaaa agatgataac agctttattc gtgggctgtc agaatttgcc
    11101 ctgaattatg atgtagatgc tgaaattgaa actcaaaaga ggtttctaga agaaaatccg
    11161 aataatgcaa agggttactt caaccttgga gtgctttatt actttcaagg aaaagtagaa
    11221 gatgctatta attcatacac taaagctctt gaggttgatc caaatttatc tgaagctcat
    11281 aagaatcttg gtgaaatcta tg
//
LOCUS       DATDRL010000305         3696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2216159, whole genome shotgun sequence.
ACCESSION   DATDRL010000305 DATDRL010000000
VERSION     DATDRL010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216159"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..840
                     /locus_tag="VI935_10270"
     CDS             250..840
                     /locus_tag="VI935_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HLE26020.1"
                     /translation="MINADQALQILMEGNKRFMAVKPLYPNLTPKRRKELSKGQQPFA
                     IIFGCVDSRVPPELVFDRGLGDLFVIRTAGQALDDAALGSIEFGVEKFNIPLVMVLGH
                     EKCGAVAATIEVVEKKAVAHGQINTLVEIIKPAVGKVMGQPGDLLENSVRANIQLTVD
                     KLKASPLLSKYLDKGKIKIVGARYDLETGVVDIIAP"
     gene            complement(936..1628)
                     /locus_tag="VI935_10275"
     CDS             complement(936..1628)
                     /locus_tag="VI935_10275"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HLE26021.1"
                     /translation="MNNEDFKDLVIQVLKEHEYDVFESDKRNQEKASSFILESHKTEK
                     HRWHVKSWDLFECNKQDKPVDLDDLKNFYELVLSDNAERGYVITTSSFTEDAKKFVKD
                     KSVELIDGRAFLSLMLKASSSGEQCNECLKIPTSIRMSLKNLRARIEVLNRFQHEISG
                     KWIAPLSLDLMLGDIVRKIKASFRTASKLGKKRLERKLPVKINNIISSVARMTKEIKS
                     FEKMVQEFSKEE"
     gene            complement(2166..>3696)
                     /gene="murJ"
                     /locus_tag="VI935_10280"
     CDS             complement(2166..>3696)
                     /gene="murJ"
                     /locus_tag="VI935_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HLE26022.1"
                     /translation="IVGVITLLSRIMGYARDMVVAYFFGARAETDAFYVAFRIPNLLR
                     RLLAEGSLTVSFIPIFTESLEKKGKEEAKKISDITFTLLLIVLILVSLVGVLLSPFII
                     KLFASGFKGEVFNLAVNLNRIMFPYILFASLTALSMGILNSLKHFFAPAFSPVIFNIV
                     VIATVFLIQQSFDLPIFSLAIGVMIGGFIQVAFQTPFLRNRGFLYEFRKSLRHPAVKQ
                     IALLMVPQLFGLAVYNLNILVSTQYASHLPEGTVSYLYYSERLIEFPLGIVAVSIATA
                     LLPSLSSQASKGELERFRASYSFALRLMLFVMVPALTGLIALRVPICNLLYQRGEFSY
                     EATIFTSQALLGYAVGLWAVGGVRITAPTFYAIQDTKSPVMIAFLAFIVNAIFGYFLG
                     FTFGLKHTGLALASSISSTFNFSLLLFTLSRRMGEIEIKKILIQVLKITVASLIMGVS
                     AWKISAFYDWSQSGISLEKIGVFLISVLVSVALYFTIAKVLKVEEIGFLFDMIKRKAR
                     I"
BASE COUNT         1230 a          700 c          671 g         1095 t
ORIGIN      
        1 tgtgagcaag tataatcctt caacatgggg catacctaca gttttctatc ataatagggg
       61 ttgcgtcccc tttttaattt aatccggtat ccatgaaaac ctgaaggttt ccgctatatc
      121 acgcttaaca tttggaatct atttttttta atggtattaa attaagatgt tcgtctgcaa
      181 taagcatgtt tttagcaact tttaaattca tccataagtc acgagcaaca ccaaaagagg
      241 gaggttccta tgataaacgc tgatcaagct ctacaaatat taatggaagg aaacaagcga
      301 tttatggcag taaagcctct atatcctaac ctaacaccca agcgacgtaa agaactatct
      361 aaaggtcaac agccttttgc aataatcttt gggtgcgtgg actcaagagt gccgccagaa
      421 ctcgtttttg accgtgggct aggagatttg tttgttatcc gaacagccgg tcaagcccta
      481 gacgacgccg ctcttggcag cattgaattt ggagtcgaaa agtttaatat accacttgtg
      541 atggtactag gacatgaaaa gtgtggcgca gtagccgcta ccatcgaagt tgttgaaaaa
      601 aaggctgtcg ctcatggcca aatcaacaca ctagttgaaa tcataaagcc agccgtagga
      661 aaggtgatgg gacagccagg cgatttgctt gaaaattctg ttagggcaaa catacaactt
      721 actgttgaca aattaaaggc ctcgccattg ctatcgaaat acttagacaa aggcaaaatc
      781 aagattgtcg gggcacgtta tgatctagaa accggagtgg tagatattat tgcgccgtga
      841 tacaaggcaa cggcacagag ggtatcgata ttttaataaa tattttaaat attctaaggt
      901 cccacttatt tatcatattg acctggtatg cgttattatt cctctttaga aaattcttga
      961 accatctttt caaacgattt aatctccttt gtcattctag caacgctgga aataatattg
     1021 ttaatcttta cgggcaattt tctttctaaa cgcttttttc ccaatttcga agcagttcta
     1081 aagctagctt ttatttttct tacaatatcc cctaacatca aatcaagact aagcggggct
     1141 atccatttac cagaaatttc gtgttgaaat cggttaagta cctcgatcct tgcccttaag
     1201 ttttttaagg acattcttat acttgtagga atttttaggc attcattaca ctgctctcca
     1261 gacgaggatg ctttcaacat tagacttaaa aaagctcttc catcaataag ttcaaccgac
     1321 ttatccttga caaatttttt tgcgtcttct gtgaagctac ttgttgtaat aacatatccg
     1381 cgctcagcat tatcagacag aacaagctca taaaaattct ttaaatcatc taaatccaca
     1441 ggtttatctt gcttgttaca ctcaaacaaa tcccacgact taacatgcca tctatgtttt
     1501 tctgttttat gtgattcaag tatgaaactg gaggcctttt cttgatttct tttgtcagat
     1561 tcaaacacat catactcgtg ttcttttaat acttgtatta ccaaatcctt aaaatcttcg
     1621 ttattcatat tcgtaaggta atccaaagat aacgagtacg gttcaattct tatgtattca
     1681 acccgacaaa gagcgcagcg atcaaatttt atagcagatt tgttgtatgg catcttctca
     1741 actatgaatt aaaaaaagtg ttatagaaaa tcataattta tctcttgtca aatctaaacg
     1801 ccaattaaaa tcctatgttt tagttaattc tgtttagaat ataagacaaa agaatgtgaa
     1861 ctggaacatc taatgtcata cactctacgc tgggcagcat accggtctga agtgactttg
     1921 taccagaagt aaacttaatg gttgggactt agcaattttt tttaaagtat acttgagaat
     1981 aatttaagtt tctaaaggtt aacaagaaat actattgaat aaagcataca attaactatt
     2041 ttgaaagtac gacggtcatt ttaatgccta tggaaatcaa atttttgggc ttgcggttgc
     2101 agaggtaatt aagagttatc tagatacttc cctataaata ggtaaataat gtttcacacc
     2161 tgcatctaaa tccttgcttt tcttttgatc atatcaaaaa gaaaaccgat ttcttcaact
     2221 ttaagaactt ttgcaatagt aaaataaagt gctactgaaa ctaaaaccga gatgagaaat
     2281 accccaattt tttctaggga aattccgctc tgagaccaat cgtagaaagc tgaaatcttc
     2341 caagctgaaa cacccataat tagtgatgcc actgttattt taagaacttg tattaatatc
     2401 ttttttatct ctatctctcc catacgccta cttagggtaa ataagagaag agaaaaatta
     2461 aaagtagagg aaatcgagct tgcaagtgca agccctgtgt gcttaagccc gaaggtaaac
     2521 ccaagaaagt acccaaatat ggcatttact ataaaggcta agaaggcaat cattacaggg
     2581 ctcttggtat cttgaatagc ataaaaagta ggggcggtta ttcttactcc acctacagcc
     2641 caaagcccaa cggcatatcc aagaagtgct tgggatgtaa aaatagttgc ttcatatgaa
     2701 aattcacctc tttgataaag aagattgcag ataggaactc taagtgctat tagacctgta
     2761 agtgctggga ccatcacaaa gagcataagc cttagagcaa atgaataact tgctctaaat
     2821 ctctcaagct cccctttcga tgcttgggag gaaagactag gaagtagtgc agtcgcaatt
     2881 gatactgcta caattcctag agggaactct atcagccttt ctgaatagta cagataagag
     2941 acagttcctt ctggaagatg agatgcgtac tgagtactta caagaatgtt taagttataa
     3001 acagcaagtc caaaaagctg aggaaccata agaagcgcta tttgttttac tgctggatga
     3061 cggagactct ttctaaattc atagagaaaa cctctattcc ttaggaaagg ggtctggaat
     3121 gcgacttgaa taaatcctcc tatcattacc cctatggcaa gagagaaaat cggaagatca
     3181 aagctttgct gaatgagaaa aaccgtagca atgaccacaa tattaaaaat tacaggtgaa
     3241 aaagcaggag cgaaaaaatg ctttagagag tttaaaatcc ccatcgaaag cgcagtaaga
     3301 gaagcaaaaa ggatataagg aaacattatt cgattgagat ttacggcgag attaaaaacc
     3361 tcacctttaa aacctgaagc gaaaagttta attataaaag gagaaagcaa aacccctacg
     3421 agagaaacca gaattagaac tataagaaga agtgtaaacg taatatcaga aatttttttg
     3481 gcttcctctt tacctttttt ctctaaactt tctgtaaata ttgggatgaa ggaaactgtg
     3541 agtgagcctt cggccaggag ccttctgaga agatttggaa ttctaaacgc aacataaaat
     3601 gcatctgtct cagctcttgc gccaaagaaa taagctacca ccatatccct tgcatagccc
     3661 attatgcggc taagaagtgt aataacccct acaatc
//
LOCUS       DATDRL010000306         2967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2679258, whole genome shotgun sequence.
ACCESSION   DATDRL010000306 DATDRL010000000
VERSION     DATDRL010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2679258"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VI935_10285"
     CDS             <1..252
                     /locus_tag="VI935_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26023.1"
                     /translation="GIASSPSTSLRVPRNDRRAVRLYDDTLQLTGCPTIMDGKNLTPQ
                     NAPGSTIGNLGGVLLPPRMPIFAFIGQPPYRRVFHLTQV"
     gene            276..1121
                     /gene="nadC"
                     /locus_tag="VI935_10290"
     CDS             276..1121
                     /gene="nadC"
                     /locus_tag="VI935_10290"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HLE26024.1"
                     /translation="MSEIQLNLHEVNQILENALREDTGSGDITTNLLFPDDAECKAII
                     LAKEEGMVAGLPIADMVFRKLDKDIVWSEEKKDGDKVESNQVLAEISGSQKAVLTGER
                     VALNFLQRLSGIATFTSRFVKAIHGLPVKILDTRKTAPGLRILDKYAVRIGGGYNHRF
                     GLYDGLLIKDNHIKLAGGITKAVNILRERAVTRKRIEVEASTISEVEEALNVGADIIM
                     LDNMSPKMIIFAVGLIKGKALIEVSGGVTLENVREIAETGVDFISVGLLTHSPEALDI
                     GLYVV"
     gene            1211..2155
                     /gene="nadA"
                     /locus_tag="VI935_10295"
     CDS             1211..2155
                     /gene="nadA"
                     /locus_tag="VI935_10295"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HLE26025.1"
                     /translation="MENDLIREEIQGLKKKRNAVILAHNYQVGEVQEVADFTGDSLGL
                     SQAAARTEADVIVFCGVHFMAETASIICPDKKVLIPDLEAGCSLASTINADELRNWKK
                     EHPDAVVVSYVNTSAEVKAESDYCCTSTNAVRVVNSIPEDKEVLFLPDMFLGAYVSKV
                     TGRKLHIWPGECHVHAGIKPEQISKIRDLHPEAEMLIHPECGCVTSCMYYTAEGSIEN
                     KNTHILSTEGMIRHVKRSKAMEFIVATEIGILHKMKKETSDKTFYPANDEAVCRYMKM
                     ITLNKVLSSLKDDIYEVKVPKELAFKAKKAIERMLKLA"
     gene            2241..>2967
                     /locus_tag="VI935_10300"
     CDS             2241..>2967
                     /locus_tag="VI935_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26026.1"
                     /translation="MNKAKWLLIFLLAVLVAVPSYAVELTLGGFPSYFRIRPQFYKNS
                     TFVGALDDAQAQALGFNDADDNIVFVDSRLRLTPQLVLSDAVTIRAQVDVMDNTIWGG
                     TNNLLVRDVVYESLTPTDRFRGALLVGPVNGNVLFNGLFGAGNGVLSSPAVDDVQYFN
                     VRMLHVDIVLPHNLGFIRAGRQPFDWGLGILANGGWDPLSDLGFVLDRFLYLKSWPLM
                     NGTFTFVFVSDRLRQGTNAFGGLE"
BASE COUNT          866 a          530 c          712 g          859 t
ORIGIN      
        1 gggattgctt cgtccccttc gacttcgctc agggttcctc gcaatgacag aagggctgtt
       61 cgtttatatg atgataccct gcagcttaca ggctgtccga caattatgga tggtaaaaat
      121 ttgacccctc agaatgcccc aggatcaacg atcggcaatc tgggaggggt tttgctaccc
      181 ccaagaatgc cgatttttgc atttatcgga cagcctcctt atcgcagggt ttttcatttg
      241 acacaagttt aatatccgtt taaaaatcga atatcatgtc tgagatccaa ctaaatttgc
      301 atgaggtcaa ccaaattcta gaaaatgccc tccgcgagga tactggaagt ggcgatatta
      361 ctaccaacct gctttttccc gatgatgcag aatgcaaggc aatcatactc gccaaggaag
      421 aagggatggt tgctgggctt cctatagctg atatggtttt tagaaaatta gataaagata
      481 ttgtctggag tgaagaaaag aaagatggag acaaggttga gtcaaatcag gttctagctg
      541 aaatctctgg ttctcaaaag gctgttctta caggcgagcg cgtggctctt aattttcttc
      601 agcgcctttc aggaattgcg acttttactt ctaggtttgt caaagctatt catgggctac
      661 ctgtaaaaat attggataca agaaaaacag cccctggact tagaatctta gataaatacg
      721 cagtcagaat tggcggaggc tataaccatc gttttggact ttacgacgga ttactaataa
      781 aggataatca cataaaactt gcaggtggga ttactaaagc agttaatatt ttaagagaaa
      841 gagcagtaac tcggaagaga atagaggttg aagcttcaac aataagcgaa gtggaagaag
      901 cacttaatgt aggtgccgat attataatgc ttgataatat gtccccaaaa atgataatat
      961 ttgctgtagg actaattaaa ggtaaagcac tcatcgaggt ctcaggggga gtgacccttg
     1021 aaaatgttcg tgagattgca gaaactgggg ttgactttat atctgtaggc ttgcttacac
     1081 attccccaga ggctttagac atcgggctct atgtggtatg atatttatga ttagttttta
     1141 gtctctagat cttagttgaa agactaatta ctaatagcta acgactaatg gctaaattag
     1201 gtgattgaag atggaaaacg atttgattag agaagagatt cagggtttaa agaagaagag
     1261 gaatgctgtg atacttgccc ataattatca ggtgggcgag gttcaggagg ttgctgattt
     1321 tactggtgat tcgcttggcc tctctcaggc tgcggcaagg acggaggcgg atgtgatagt
     1381 tttctgtggt gtccacttta tggctgagac ggcttctatt atttgtccgg ataagaaggt
     1441 gttaatacct gatttagagg ctggatgctc acttgcttct acaattaacg ctgatgagct
     1501 aagaaattgg aagaaagagc atcccgatgc tgtggttgtt tcttatgtga ataccagtgc
     1561 agaggttaag gctgaaagtg attactgttg cacatcaaca aatgcagtta gggttgtgaa
     1621 ttcaatccca gaggataaag aggtcctttt tctgcctgat atgtttctcg gggcttatgt
     1681 ttcaaaggtt accgggagga aacttcatat ttggccagga gagtgccatg tacacgcggg
     1741 gataaagcct gagcaaattt caaaaataag ggatcttcac cctgaggccg aaatgcttat
     1801 tcatcctgag tgtggctgtg taacttcctg catgtattat acggcggagg gatcaattga
     1861 aaataagaat actcatatac tctcgaccga gggtatgatc agacatgtaa aacgttcaaa
     1921 ggcgatggag tttatagtag ctactgagat aggaatactt cacaaaatga aaaaagaaac
     1981 ctcagataaa accttctacc ccgcaaatga tgaggccgtg tgcagataca tgaaaatgat
     2041 taccttaaat aaggtattaa gctctctaaa ggatgatata tatgaggtga aggtacctaa
     2101 agaattagca tttaaggcta aaaaggctat tgaaaggatg ttaaaactag cttaaaaatt
     2161 ataaaaaaaa accccttgac aaaggtggat attttatata atgtaaactc agaccttaaa
     2221 ataaaaggag gtaaggagag atgaataagg ccaaatggtt actaatattc ctactggctg
     2281 tgttggtagc cgttccatcc tatgcagtag aactaacctt gggcggcttc cccagctatt
     2341 tccgtatacg tcctcagttt tataagaatt cgactttcgt cggtgcatta gatgatgctc
     2401 aagctcaggc gcttggcttt aatgatgcag atgacaacat cgttttcgtt gactcgaggc
     2461 tccgtttaac ccctcagctt gtgcttagtg atgcagtgac aattagagca caggtggatg
     2521 ttatggacaa tacaatctgg ggcggtacaa ataatcttct tgtaagggat gtggtgtatg
     2581 aatccctaac ccctactgat aggttccgag gtgcactgct tgtaggacca gttaatggga
     2641 atgtgttatt taatggtctc tttggagccg gaaatggtgt tctatcaagt ccggctgttg
     2701 acgatgtcca gtactttaac gtccgtatgc tccacgttga cattgttctt ccacataact
     2761 tgggatttat aagggcaggg cgtcagccat ttgactgggg tctagggatc ttggcaaatg
     2821 gtggttggga tccactatca gaccttggat ttgttcttga caggttcctt tatcttaagt
     2881 cctggccttt aatgaatgga acctttacat tcgtattcgt tagtgacagg ctccgtcagg
     2941 gtactaacgc attcggaggt ttagaaa
//
LOCUS       DATDRL010000307         2818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1426733, whole genome shotgun sequence.
ACCESSION   DATDRL010000307 DATDRL010000000
VERSION     DATDRL010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426733"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VI935_10305"
     CDS             <1..203
                     /locus_tag="VI935_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HLE26027.1"
                     /translation="VRLGVYYLSMLINRFKSVEWALHAYNAGPSFINTASLERGIPAY
                     VRKVLRFKIILESQRIAEEQSL"
     gene            complement(224..562)
                     /locus_tag="VI935_10310"
     CDS             complement(224..562)
                     /locus_tag="VI935_10310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HLE26028.1"
                     /translation="MEIKKWHVYLADLNPRIGTEPGKVRPVVVVQTDLLNGLHPSTVV
                     CPLTTKVQPRARFLRVHLTIGEAGLKEQSDIMVDQIRAIDNRRLLRVVGKISRKSQVK
                     LIENLRILLT"
     gene            complement(569..808)
                     /locus_tag="VI935_10315"
     CDS             complement(569..808)
                     /locus_tag="VI935_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26029.1"
                     /translation="MSKLLSLKLRDKVFLDAEEILRRSRKPRNAYFNEAINLYNKLWK
                     RKLLKKTLAEESAMVADESLLVLEAFELLEYELDK"
     gene            1064..1221
                     /locus_tag="VI935_10320"
                     /pseudo
     CDS             1064..1221
                     /locus_tag="VI935_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874720.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            1947..2195
                     /locus_tag="VI935_10325"
     CDS             1947..2195
                     /locus_tag="VI935_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26030.1"
                     /translation="MITPELKKNAEGVLKWMQKHTTATQAGGGVSHGEIAQESGLTQK
                     DVDEVIDYLTKRSFIAGSADRFWLTPLGKMIQTITDEQ"
     gene            complement(2224..2373)
                     /locus_tag="VI935_10330"
     CDS             complement(2224..2373)
                     /locus_tag="VI935_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26031.1"
                     /translation="MKKTFYCIRCHLVYEESDCTSEDEAVCDKCGGELAVLDKDIDLD
                     EGGES"
     gene            2485..2721
                     /locus_tag="VI935_10335"
     CDS             2485..2721
                     /locus_tag="VI935_10335"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HLE26032.1"
                     /translation="MPLNAYVLIHVSGPQTMSVLRKIQKFKGVMSAHVVVGPYDVIAF
                     VEGDNHKEIGDLVFTKIRRVQGVTATTTCFAMEV"
BASE COUNT          890 a          528 c          594 g          806 t
ORIGIN      
        1 atgtgaggct tggtgtttat tatctttcta tgttgattaa ccgttttaaa agcgttgaat
       61 gggctctgca cgcttataac gcaggaccca gctttataaa cacagcaagt ttagaaagag
      121 ggattccagc ctatgttaga aaggtgctca gatttaaaat tattctagag tctcaaagga
      181 ttgcagaaga acagagtttg taacacctga acgactctat agcctatgtc agcaaaatcc
      241 taagattctc aattagcttt acttggcttt tacgagaaat tttacctacg acacgaagta
      301 aacggcgatt atcaatggct cgaatctggt ccaccataat atcagactgt tcctttaacc
      361 cggcttcgcc aatagtaaga tgcaccctta ggaaacgcgc ccttggttgg acttttgtag
      421 ttaagggaca tactacggtt gatggatgta gcccattcag aagatcggtc tggacgacaa
      481 ccactggacg aaccttccca ggttccgttc caattcgtgg gttgagatct gcaaggtaaa
      541 catgccactt tttgatctcc atagcgattt acttatccaa ttcatattcg agtagttcga
      601 atgcctccaa gactagaagg gattcatctg caaccattgc ggattcttct gctaaggttt
      661 tcttcagaag ctttcgcttc cagagcttgt tatagaggtt aatggcttca ttgaaatagg
      721 catttctggg ctttcgactc cgacgaagga tttcttcagc atccagaaac accttatccc
      781 gtaattttaa tgagagcagt ttggacatat caaattcccc atcgaaagat ttaagtatca
      841 ccataagtat atacgcaagt aaagagacat caacaaagtc aataaagtct caactccccg
      901 aagggaatta tatgattttc cacatctttc tgtaggggct gaacaccgtt cagcccatct
      961 gtttaaattc ccgatgggaa ttagaagtcc ttctacttca ttaaatgatg ttagaattta
     1021 tgtgtaaatt ggtcaactca aatcaccatt aaaggggaaa actatgcaaa ttaaagtgat
     1081 attagaacct gctgaggaag gagatatact gtatatgtgc cttcccatcc tggatgtatc
     1141 agcgaaggag aaacggaaga agaggcgctg actaatatta aagaagcaat agaactttat
     1201 ttagagccga ttgatgatga aaagtttaaa cgagaattga gatgctattc taaggccgat
     1261 gataaactta gagcaattta tgaacatggc tatcaagcag acttcgccaa taatcaagat
     1321 aattggtcag gtgccatggg cagggaaatc acaataatag ttggaatgat ggagtacata
     1381 tatccagaca tagatgttat gcttgggggt gtgtggagct cgattagtag aatttttagt
     1441 atttataatg catacctaac ccctgttaag aacccagagc gttttaacga acatgaatat
     1501 atcaatttct atatcgacct catggaaaaa tataataagg gggatacaga taaccaccac
     1561 gatccaacag atttagtgtt gtcagtaata ggtcatgctc attccccaag gttagtaaaa
     1621 aaaccaaaaa acgaaagaat gtattatctt atggattgtg gttcttgggt gaatggtggc
     1681 catgaatttg gggtaatatc aggaagagag atggcagttt gtcaatgggg agatgaaacc
     1741 aaacaaacga tcaagcctaa aaaatcagat aaaaaggcac cgactgcccg aataatgaga
     1801 aagaagtaag ggtcaagatg cttaacaaat taattgagct taagaaggta atgaaactaa
     1861 ttaagaacct cgcctcgatg gttttggggc acgaatgagt atatactctt ttcgattctt
     1921 tgagttgaaa tagaaggtgt gggaaaatga ttacacccga gcttaagaaa aatgcagaag
     1981 gagtactaaa atggatgcaa aaacatacaa cagcaacaca agctggaggc ggggtgagcc
     2041 acggtgagat agcccaggag agtgggttaa cacagaagga tgttgatgaa gttattgatt
     2101 acctgaccaa gcgtagtttt atagcagggt ctgctgatcg attctggtta acccccttag
     2161 gtaaaatgat tcaaaccatc actgatgagc agtagttgcc cgaaaaatat agtctctttc
     2221 cctttaactc tctcctccct cgtctaaatc aatgtcttta tctagtacag ctagctcacc
     2281 accgcactta tcgcatactg cttcatcctc tgaagtgcag tcactctcct catatactaa
     2341 atgacatctt atgcaataga aggttttttt cattttagtt ctaccatcct tattcaatcc
     2401 tcatccccta aatttattcc ttagcttcag gtatagctct agtgtatatt ttaactataa
     2461 atccgagaag aaaggagagg gtagatgcct cttaatgctt atgtacttat acacgtatca
     2521 gggcctcaga caatgagtgt cttacgtaag atacagaaat ttaaaggggt tatgtctgcg
     2581 catgtagtgg tgggtcctta tgatgtgata gcttttgtag agggagataa tcacaaggaa
     2641 ataggagatt tagtattcac gaagatacgc cgtgtgcagg gagttacagc aaccactacg
     2701 tgttttgcaa tggaagttta aattcccccc ttcattttat agcctccttt tcttttagaa
     2761 atggtttttc atctgaaggg agcagtgtca ccgcactaaa aggatttgat accacaat
//
LOCUS       DATDRL010000308         5593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2373028, whole genome shotgun sequence.
ACCESSION   DATDRL010000308 DATDRL010000000
VERSION     DATDRL010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2373028"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1031
                     /locus_tag="VI935_10340"
     CDS             219..1031
                     /locus_tag="VI935_10340"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE26033.1"
                     /translation="MYREKFELGLNLLEQTEFEEAIEIFSEIAVDSPDNYEVWLNLGV
                     CYLETQRPDLAAEAFERAIKADPNHADAYYLLGTAKGTSGDIDKALDFYKKAVEIEPE
                     HQKANELLSRTNALIESREHYRNALRLLSAENNTRDNINLAIRELLQSISIFRNSPGR
                     HNLGQIIDDILNAKKESNLPIDTIKDENKLWAKNCEKGFRFLKKGLWESAISYYGQAL
                     SYRDHDALVHHSLGMAFFKVGAVQDAVQAWLKVLELEPDYDFTTLTNLPKFE"
     gene            1763..2029
                     /locus_tag="VI935_10345"
     CDS             1763..2029
                     /locus_tag="VI935_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26034.1"
                     /translation="MKVVIKGHASFEAKRRGISKKFIRFIVKNHQQKLPSKKGRVILQ
                     NKYYDQKEKKEMLLRVIGIETAKLFTVITVYKTSKISKYWTEEN"
     gene            2031..2243
                     /locus_tag="VI935_10350"
     CDS             2031..2243
                     /locus_tag="VI935_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002802494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HLE26035.1"
                     /translation="MKVIFDPETDTLSLIFREDKIAESDEVREGIIIDYSKDGKIVSM
                     EILDASDQISEPQGILYELKGNKAFG"
     gene            2387..2683
                     /locus_tag="VI935_10355"
     CDS             2387..2683
                     /locus_tag="VI935_10355"
                     /inference="COORDINATES: protein motif:HMM:NF042609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HLE26036.1"
                     /translation="MQKWRHPGGKLRELGADSLTDAELLAILISTGITGKPAEEVAKE
                     ILDRFGSFKGMANQPLEKFLEIKGIGDVKIIRIAAAFEIARRIVNQVLKEYDKE"
     gene            2697..2981
                     /locus_tag="VI935_10360"
     CDS             2697..2981
                     /locus_tag="VI935_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26037.1"
                     /translation="MPVAIKDVSEKLTAIIEPSEGVYVATCPELDLATEGNTPEEALD
                     DLIDMAIDYAEQYMEEFELFSKSPNRAAHKSYVLEIHEKGTKEKVRELFT"
     gene            2981..3202
                     /locus_tag="VI935_10365"
     CDS             2981..3202
                     /locus_tag="VI935_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HLE26038.1"
                     /translation="MVTYNDFRKVLKRCDFTLVRSKKHETWQKILPDGSILQVRLSHQ
                     HGKDIPKALFHKMLKQAYLTEEDFRKLLV"
     gene            3199..>5593
                     /locus_tag="VI935_10370"
     CDS             3199..>5593
                     /locus_tag="VI935_10370"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HLE26039.1"
                     /translation="MKKTKLSGKSTRVTSPFDTSSHKTERTANQKFVEWINQIIKERN
                     LPLGIAEQETVGADRKQPDIVIFESPKSEKVLCLIELKPPYFSPLDEKELKNPAWEKA
                     NKRKAKYFATSNFQWLIWFNTEKANRMEPEERQVVDKFHFSSIESLDDIEDARFKNPI
                     LRGIEKFLEELVRVHTGIKPEPLLPIDEFLVFRLQEKIYRLARYYREIIRDKVHKDSV
                     FSRKFQKWFIEQQWTFTFSDSDFEKAARQTAYLLINKILFYDVLQSKRSGQLDPLTIP
                     DDLTKGGLLEKTLQGYFDYVLKNIDYETIYSTDFIDQIAFPENRGVVEEIKELIRVLK
                     RYDFSKIGFDIIGRIFERLIPQEERHNLGQYFTNPDIVDLILGFCVKHENDKILDPSC
                     GAGTFLVRAYQHKKMMNLRILHQEVLKTLWGVDIAKFPAHLSTINLAVNDLSVDENYP
                     QILNEDFFNLTAVEKMGGDEVRKKELSTLSGKKVLIPYPRTVDCIVGNPPYTRQEEIT
                     EITGEENYKESMIEKALLLEGKRKLANISKRAGIHAYFFVHGTKFLKDGGRFGFIVSN
                     SWMDVDYGKGLQELFLSYYKIIAIIESKVERWFEDADVNTCIIILEKCKDQKERDENL
                     VRFVYLFKPLRHFIPPAHDIWEKQKQRLDAIENLKKTILFHNNFYQNDELRIYPKKQS
                     ELWKEGFDKEEGKYIGSKWGKYLRALEIFFKILEKGQDKLVPLKEIADVRFGIKTGAN
                     EFFYLTEEEIKEKGIEKEFWMHKEEKGEWVPNYIIKSPKECKSITVKQKDLKFRVLII
                     "
BASE COUNT         1925 a          995 c         1166 g         1507 t
ORIGIN      
        1 tgcaagtgaa gaagaataag attttagcca cgaatttaca ctaatgaaca caaatgaaac
       61 gacagctaaa tatttatatt ccaatggaat cctaaaggat tccgctaaag ttatatcatt
      121 tgtatgtagc tgaaagattt aactttccac aaattcatca ctctattcgt gtaaattcgt
      181 gtccattcgt gactaattag ttgcggagag gaacaattat gtatagagaa aaattcgaac
      241 ttggacttaa tctcctagaa cagacggaat ttgaggaagc gatagaaatt tttagtgaaa
      301 ttgctgttga tagcccagac aactatgaag tttggctcaa tctaggagtt tgttatttgg
      361 aaacccaaag acctgacctt gcggctgagg cttttgaaag ggcaatcaag gctgatccaa
      421 atcatgcaga tgcatattat cttctaggaa ccgctaaggg tacatccggc gacatagata
      481 aagctttaga tttctacaaa aaggcagtcg aaattgaacc ggagcatcaa aaggctaatg
      541 agttactttc aagaacaaac gcgcttattg aaagtaggga acattataga aatgctttaa
      601 gattattgag tgcggaaaac aacacccgtg acaatatcaa ccttgcaatc cgagaacttc
      661 ttcaatccat atcaatattt cgtaactccc ccggacgaca taatctaggg caaataattg
      721 atgatatact aaatgcaaag aaagaaagta atctgcctat agatacaatt aaagatgaga
      781 ataaactttg ggctaagaat tgcgagaaag gttttagatt cttaaaaaag gggttgtggg
      841 aaagcgccat tagttattat ggccaggctt taagttatcg tgatcatgat gcccttgttc
      901 accattccct aggaatggcc ttctttaaag taggtgctgt acaggatgca gttcaagcat
      961 ggttaaaggt gcttgagctt gagccagatt atgatttcac cacccttacg aatttaccga
     1021 agttcgaata atctctacga agcctgttta aaataggacc gttctccgcc atttatttta
     1081 ataatgttag gcgggactca ccagttccat atcggcaagc ttgtaggcac gaacttattc
     1141 gtgcaataaa gtaggaacgg cttccagacg caaattgaaa accgttctcg caatttattt
     1201 taatacggtt agttgaataa tagttccttc cttggtggaa tcatgatttt ttcctgagat
     1261 gaattcatat tcacaaaccc atataggcgg gattttcaaa ccccgcaagt tgctattggg
     1321 agcaaagaag tgacgaagta atccctatcg caactctata tgcgggggac tttccagtcc
     1381 cgacctcgtt atccttgtgt tacaggttga tcccacgaat taccaacata atggagtagc
     1441 caacgagtct tgctcgttag ctgtaactct aaccaagttt ttcatacacg gcattactct
     1501 gaatgagttt tcaatagcgg agattttcgc cccccaatta caactttcca tcatgtttct
     1561 gaacttatgc agggtgactc atacagtatt accctttttt ctcacggttc ttactattac
     1621 ttactatcaa accccttgaa tatttagcga aacttaatgt atcttatgca aataaaatat
     1681 taggacaaat gcttactcaa aaactgcggg gaaaaaccta atgcaaaaga ggaaactgac
     1741 tgtctttgga aggtatacat aattgaaagt tgtaattaaa gggcatgcaa gttttgaggc
     1801 aaagcgaagg ggcatctcaa agaaatttat aagatttatt gttaaaaatc accagcaaaa
     1861 attgccctct aaaaaagggc gggttatatt acaaaacaag tattatgatc agaaggaaaa
     1921 gaaggaaatg cttttaagag tgataggaat tgagacagcc aagctattta ctgtcataac
     1981 agtttataaa acatcaaaga ttagtaagta ttggacggag gaaaattaga atgaaagtta
     2041 tatttgatcc agagacagat acgcttagcc ttattttcag ggaggataag atagccgaga
     2101 gcgatgaggt gagagagggt ataatcattg actatagcaa ggatggaaaa atcgtatcaa
     2161 tggaaatcct cgatgcctca gatcaaatat ccgaacctca gggaatactc tatgagctaa
     2221 aaggcaataa agcctttggt taatttggta gtctgtttct cttttacttc tttcctgcta
     2281 accctttcac tttccctata caatctcttg agcatgtagc aaaataatta taaaattagg
     2341 acatgcgttt aaatcaaacc acgccaaaaa ctgggggata aacctaatgc aaaagtggcg
     2401 tcaccctgga ggcaaattaa gagaactcgg agcagattct ctaaccgatg ctgagctttt
     2461 agcaatcctt atctctacag gaataacagg caaacctgct gaagaggttg cgaaagaaat
     2521 ccttgataga tttggctcat tcaagggtat ggcgaaccag ccacttgaga agtttcttga
     2581 gattaagggg attggagatg taaagataat tagaattgca gcagcatttg aaattgcaag
     2641 aaggattgta aatcaagttt taaaggaata cgataaagaa tgattggagg tgaaatatgc
     2701 cagtagctat aaaagatgtc tctgaaaagc taactgccat aatagagcct tctgaagggg
     2761 tatatgttgc aacctgtcct gaacttgacc ttgctacaga gggcaatacc ccagaggagg
     2821 cactggatga tttgattgat atggcaatag attatgcaga acagtatatg gaagagtttg
     2881 aactctttag caaaagccca aatagggcag cccataaatc atatgttctt gaaatacacg
     2941 aaaaaggaac aaaagaaaaa gtcagggaac tttttactta atggtcacat ataatgactt
     3001 tagaaaggtg ctcaaaagat gtgattttac gcttgtaagg tcaaaaaaac acgagacgtg
     3061 gcagaaaatc cttccggatg gatcgatact tcaagttaga ttaagtcatc agcatggcaa
     3121 agatattcca aaggcattgt ttcataaaat gttaaaacag gcatatctta cagaagaaga
     3181 ttttagaaaa ttacttgtat gaaaaagaca aaactttcag gcaaatccac aagagttaca
     3241 agccccttcg atacatccag tcacaagaca gaacgcactg ccaatcagaa atttgtcgag
     3301 tggattaacc agataatcaa agaaagaaat ctcccccttg ggattgctga acaggagacc
     3361 gttggcgctg atagaaaaca gccagatata gttatctttg aaagcccaaa aagtgagaag
     3421 gtgctctgcc ttatcgaatt aaagccgcca tatttcagcc cacttgatga aaaagagcta
     3481 aaaaaccctg catgggaaaa ggcaaataag agaaaagcga aatactttgc gacctcaaac
     3541 tttcagtggc tcatatggtt taacaccgag aaggcaaaca ggatggaacc tgaggagaga
     3601 caggttgttg ataaatttca tttctccagc attgaaagcc ttgatgacat cgaagatgca
     3661 agatttaaaa atcctattct caggggcatt gagaaattcc ttgaagaact tgtcagggtt
     3721 cacaccggga taaagccaga gccattatta ccaatagatg agttcttagt attcagactt
     3781 caggaaaaga tataccgcct tgcaagatac tacagagaaa tcatcagaga taaggtccat
     3841 aaagattctg tgttttcgag aaagtttcag aaatggttta ttgaacagca atggacattc
     3901 acgttttcag atagtgactt tgaaaaggca gcaaggcaaa ccgcctacct actcataaac
     3961 aaaatcctat tttacgatgt gcttcagtcc aaacgatccg gacagcttga tcctcttact
     4021 atacccgatg atctcacaaa aggagggctt ctcgagaaaa cccttcaggg ctattttgac
     4081 tacgtcctta aaaatattga ctatgaaact atctacagca ctgacttcat tgaccagata
     4141 gccttccctg aaaatagagg agtcgttgaa gagataaaag agcttatcag agttcttaag
     4201 agatatgatt tctctaagat aggttttgat attattggaa gaatctttga aagattaata
     4261 ccgcaggagg aaaggcataa cttaggtcaa tattttacca atcctgatat tgttgatctt
     4321 attttgggat tctgtgtaaa gcatgaaaac gataaaatac ttgatccctc ctgtggtgcg
     4381 ggaacctttc tagtgagggc ttatcaacat aaaaaaatga tgaatctccg catccttcat
     4441 caagaagttt taaaaactct ctggggggtt gatatagcca aattccctgc ccatctttcg
     4501 acgataaatc ttgccgtaaa tgacctctca gttgatgaga actatccaca aatactcaat
     4561 gaggactttt ttaacctgac cgctgtagaa aagatgggag gggacgaggt tcgtaaaaag
     4621 gagctttcta ccctaagtgg gaaaaaggtt cttatccctt atccaagaac tgttgactgc
     4681 atagttggaa atcctcctta tacaagacag gaggagatta ctgaaataac gggggaagag
     4741 aactataagg agtccatgat tgaaaaagcc ctgctgctcg aaggaaaaag aaaactagcc
     4801 aatatttcaa agagggcagg tattcatgcc tatttctttg tgcatgggac gaaatttttg
     4861 aaggatggtg gacggtttgg tttcattgtc tcaaactcat ggatggatgt tgactacggc
     4921 aaagggcttc aagagctatt cctgagttac tataaaatca ttgcaatcat tgaatccaag
     4981 gttgagcgtt ggtttgaaga tgcagatgtc aatacgtgca taataatcct tgagaagtgt
     5041 aaagaccaga aagagcgtga tgaaaacctt gtgaggtttg tatatctctt taaaccatta
     5101 aggcatttca ttccccctgc ccatgatatt tgggagaagc aaaagcagag actcgatgcc
     5161 atcgaaaacc tgaaaaagac aatacttttt cacaacaatt tctatcagaa tgatgagctc
     5221 aggatttatc ccaagaagca aagcgaacta tggaaagaag gatttgataa agaagaaggc
     5281 aaatacatag gctctaaatg gggaaaatac ctgagggcac tagagatatt cttcaagatt
     5341 cttgagaagg gacaagataa acttgttcca ttgaaagaaa tagctgatgt taggtttggc
     5401 atcaaaacag gtgctaacga gtttttctat ctaacagaag aagagataaa agaaaaaggg
     5461 attgagaaag aattctggat gcacaaggaa gaaaagggcg aatgggtgcc gaattatatc
     5521 attaagagtc caaaagaatg caaatcaatt accgtcaagc agaaagacct aaagtttcgt
     5581 gttctgataa ttc
//
LOCUS       DATDRL010000309         5157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2184029, whole genome shotgun sequence.
ACCESSION   DATDRL010000309 DATDRL010000000
VERSION     DATDRL010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2184029"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VI935_10375"
     CDS             <1..950
                     /locus_tag="VI935_10375"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HLE26040.1"
                     /translation="KECMFPNPGYGASFYCHIKLCNVYIIVWELGIRNWELVNEKKTW
                     KTKMKRIITLITDFGLKDHYVGAMKGVILSINPDAIITDITHEIPPQDIFNAAFILRN
                     LYHYYPKGTIHIVIVDPGVGGKRKPIAVEADKNIFLGPDNGILTFIYLESKSVRVFEI
                     SNSKYTLPKLGSTFDGRDVFAPTAAHLSLGVPVDDLGGQVKKPVKLQIKEPVVRGSEI
                     IGEVIYIDSFGNLITNIPAQLIKPKLRVYIGGEVIKGISQSYAEVPKGELLAIIGSAG
                     LLEISVSHGSALDVIETWLGSTKSKKVKKVKHIPCVIRI"
     gene            1719..2888
                     /locus_tag="VI935_10380"
     CDS             1719..2888
                     /locus_tag="VI935_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26041.1"
                     /translation="MVDFISSLPDYLRKFVVPSLPKEQRLNAARGLIAIPSKDLVKVL
                     YSLILDEDEEISKEAKASLKSMPDGVIGTVLDDTSTSSKLLDYIARNTTSEFQLQKII
                     LNPATSDDTIAFLAEKVYSTPIIGLITDDQKRILGSEVIVEALTKNPSISRSKLDRVI
                     SVFKLYLAKKGEASLNYPKYDSKNDSIVDVSKDAIDSDLSSEDEEEEAKSTFLDDVDE
                     SFLDGIDFSSDLTEETGEEINEAIERKPQGLLHKIRQMNIPQRIKLALLGNKEARAIL
                     VRDSNRIVAVSALMNPRVTDMEIMLIAQSKTANEDVLSQLASTRRWTRNYQVKVALVN
                     NPKTQTPLALRFIRHLRDKELQALSGNKDVPGVIASLAMRLYQERINWKTGGRKH"
     gene            2939..3895
                     /locus_tag="VI935_10385"
     CDS             2939..3895
                     /locus_tag="VI935_10385"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HLE26042.1"
                     /translation="MSKEEPIERFQIPKDFSGKRADVAISSLLTSITRSQIKRMLDEG
                     LILVEGKPIRPSKKLSGGESVTVTFAPPESIEARPQDIQVPIIYEDNDIIVVNKPWGI
                     TVHPGAGIKDGTLVNALLYKCLGLSGIGGKLRPGIVHRLDKGTSGVMVVAKNDFAHNS
                     LVNQFKSHETKKSYLALVVGDIKGETGSFSSLIGRHPKNRIKMTTKVRAGREAFTLWR
                     VTKRYKEVTMVEVEPKTGRTHQIRVHFAENGYPILGDKMYGVKKHKSELLERLSKCLA
                     RQALHASTLNFKHPRTGEYVEFSAPIPDDIKAVIKLLENELD"
     gene            3882..4562
                     /gene="pgeF"
                     /locus_tag="VI935_10390"
     CDS             3882..4562
                     /gene="pgeF"
                     /locus_tag="VI935_10390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HLE26043.1"
                     /translation="MSWIKSQSLSEIGGIVHGFSNKKFNGSVSDIARYFGLNNIATID
                     QTHSSDVLVINDGFKDLSREKGDAIITDLRGIGIGVFTADCVPILFAEKAGSLVAVVH
                     AGWRGTLSQVPIATLTEMKKRFGIEPSRVSCVVGPSIGGCCYEVGEDVASQFIHKFDN
                     WKDFLFRKNNLKYILDLKGANRINLSKEGVKDIEVINICTKCDSDFHSYRRDGENAGK
                     QLSFAGLV"
     gene            4636..5076
                     /locus_tag="VI935_10395"
     CDS             4636..5076
                     /locus_tag="VI935_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26044.1"
                     /translation="MFQIMCVLTSEDYWYLDSMKESNIPVNFYGYTFEVEGGGEAEGF
                     GRVKLMVIELINARMAVGFAIPRNMSVDGEFELRFLCHESPTNEIPIVCKLSQEVKRT
                     TYRGDDDAKLEYIGFSLEKFYESKGATFYFYDLRGSQLDKQMSK"
BASE COUNT         1597 a          875 c         1127 g         1558 t
ORIGIN      
        1 ataaggaatg catgttcccg aacccaggat atggagcttc attttattgt cacattaaat
       61 tgtgtaacgt ttatattata gtttgggaat tggggattag gaattgggaa ttagtgaatg
      121 agaagaaaac atggaaaact aaaatgaaac gcatcataac cttaataacc gattttggat
      181 taaaagatca ctatgttgga gcaatgaaag gggttatact ttcaataaat cctgatgcaa
      241 taattactga tatcacgcat gaaataccgc ctcaagatat ttttaatgct gcttttattc
      301 tacggaatct ttaccattac tatcctaaag gaactataca tatcgtcatt gttgatccag
      361 gtgttggtgg taaaagaaag ccaatagcag tagaggccga taaaaatatt tttctagggc
      421 ctgataacgg aattttaacg tttatttatc ttgagtcaaa gtcagttagg gtattcgaaa
      481 tctcaaattc caaatacact cttcctaaat tgggttctac ttttgatggt agggacgttt
      541 ttgctcctac cgccgcgcac ctttctctcg gtgtaccggt agatgattta ggcgggcaag
      601 taaagaagcc cgtaaagctt cagattaaag agccagtggt taggggaagt gaaataatag
      661 gcgaggttat ttacatagac tcttttggaa accttattac gaacatacct gctcagctca
      721 taaaacctaa gttaagggtt tatataggtg gtgaggtaat aaagggaata tcacaatctt
      781 atgcagaagt tccaaagggc gaacttctag cgatcatcgg aagtgcaggt ttactggaaa
      841 tatcggttag tcatggcagt gccttggacg tgattgaaac ttggcttggt tctacaaaat
      901 ccaaaaaggt aaaaaaagta aaacatatac cctgtgttat tcgaatttaa tttgataatt
      961 ttctgtaact tgctacgggg tttctactgt cattggcaga aactgatggg gttggttagg
     1021 ttttcaaccc ttgcaagtgg tgctcttttc tgtcattgcg agcgttagcg aagcaatcca
     1081 ttgctaacac tatgtaggtt cattgcaccc ctctgcctga ctattcaagg gattgcttcg
     1141 gtcgtttcac tcactcgcaa tgacatttcc gtgttatcat tgcgaacaca ttcgcggagc
     1201 ctgtatagag gaaacgaagt actcagtgta aactccgtga agctatctcc tgctatttaa
     1261 atgtagcgga ggtctttaga cctccaatta catattttgt tttgggcgac ctgaaggtcg
     1321 ccgctacctt gttaaaacta gggattgctt ctgtcgctgc ctttcatttc catttctcgt
     1381 tcttttcatt tccctttgaa ccacttattt ccatttgatg cgcactgcgc atcaaataat
     1441 acactgcggt tcaaataata ctgtgctcga aataattctc ctcgcaaatg acggaaagag
     1501 ttgtctgtct aaacgctgat accgtacggg ttgctccaat ggcttcattt tccaattcca
     1561 tcttgaactt tacttttgac tccgatttgc ttatgcgctg attgagaaac atgcactcaa
     1621 ggaaatagtc tcattttttt tctactccag tatcaaatca tcggttgtca tgtgtttgta
     1681 tacatcggtt tttttacata aaataaatct aataaacgat ggtagatttt atttctagcc
     1741 ttcctgatta tttaagaaaa tttgtggttc cctctcttcc aaaagagcag cgccttaatg
     1801 cggcacgggg ccttattgct attccgtcaa aggatcttgt caaggttcta tatagcctga
     1861 ttctggatga agatgaagaa ataagtaaag aggctaaggc ttctctaaaa tcaatgccgg
     1921 atggagtaat tggaacagta cttgatgata catcaacttc atctaagctc cttgattaca
     1981 ttgctagaaa tacaacgagt gaatttcaac ttcaaaagat aatactaaat cctgccacaa
     2041 gtgatgatac catcgccttt cttgccgaga aagtttatag tactcccatt atcggattga
     2101 taacagatga tcaaaaacgt atcctagggt cagaagttat agtcgaagca ctaaccaaga
     2161 acccttctat aagtcgctct aaactagata gggttatttc cgtctttaag ctttatttag
     2221 ctaaaaaagg tgaagcttct ctcaattatc caaaatatga tagtaaaaat gattcaattg
     2281 tggatgtttc taaagatgct attgattctg atttatcgtc agaggatgaa gaagaggaag
     2341 ctaaatctac ttttcttgat gatgtcgatg aatcttttct ggatggcatt gatttctcta
     2401 gtgatcttac agaggaaact ggagaagaga taaatgaggc cattgagcga aagcctcagg
     2461 gtctgcttca taaaataagg cagatgaaca ttcctcaaag gatcaaactt gctcttttag
     2521 gaaataaaga ggcacgagcg atcctggttc gggattcgaa tagaatagtg gccgtttctg
     2581 cgttaatgaa cccacgtgtt accgatatgg agattatgct aattgctcaa tcaaaaaccg
     2641 caaacgaaga tgtgctaagt cagttagctt caactcgtag atggacaagg aactatcagg
     2701 tcaaagtagc tttggttaac aatcctaaga cacaaactcc tttagccctt cgctttataa
     2761 gacacctaag ggataaggaa cttcaagcac tttcagggaa taaggatgtt cctggagtaa
     2821 tagcaagctt ggctatgagg ctatatcaag aaaggataaa ttggaaaact ggtggaagaa
     2881 agcactgatt attcgcgata aaagcatttt tataaataga ctatccaagc aattaaatat
     2941 gagtaaagaa gaacccatag aaagatttca aataccaaaa gacttctcgg gcaagagagc
     3001 agatgttgct atttcgtctc ttcttacaag tattacccgt tctcaaataa aaagaatgtt
     3061 ggacgagggt cttattttag tagagggcaa accaataaga ccgtccaaga aactaagcgg
     3121 gggtgagagt gttactgtaa cttttgctcc acctgaatct atagaggcta ggcctcaaga
     3181 tattcaagtt ccaatcatat acgaggataa tgacattatt gtggttaaca agccttgggg
     3241 aataacggtg catcctgggg ctggaataaa agacggtact cttgtaaatg cgctccttta
     3301 caaatgtctt ggtctttcgg gaattggggg aaaacttaga ccggggatag tgcacaggct
     3361 tgacaaaggc acctctggcg ttatggtagt cgccaagaac gactttgctc ataattctct
     3421 tgtaaatcaa ttcaaatcgc acgaaacaaa aaagagttat ttggcattag tagttggtga
     3481 tattaaaggg gaaacaggtt cattttcttc tctcatagga aggcacccta aaaatagaat
     3541 taagatgaca acaaaggtca gagcaggaag agaggcgttt actttatgga gggttacaaa
     3601 aaggtataaa gaagtgacaa tggttgaagt tgagcctaaa actggaagaa ctcatcagat
     3661 aagggttcat tttgctgaaa atggttatcc gatattgggt gataaaatgt atggtgtaaa
     3721 aaaacataaa tccgagttgt tagagaggct ctcaaagtgt cttgcaaggc aggcacttca
     3781 tgcgtcaacg cttaacttca agcacccaag gactggagag tatgtggaat tttctgcccc
     3841 cattcctgat gatattaaag ccgtgattaa attattagaa aatgagttgg attaagtctc
     3901 aaagtttatc tgaaatcggc ggaatagttc acggattttc taacaaaaaa ttcaatggga
     3961 gcgtatctga tattgcaaga tatttcgggt taaataacat tgccaccata gatcaaacac
     4021 atagcagtga tgttttagtc ataaatgatg gattcaagga tctgtcaagg gagaaagggg
     4081 atgcgattat aactgattta agaggaatag gtattggggt ttttactgca gattgtgttc
     4141 ctatactttt tgcggagaaa gctggttctt tagttgctgt agttcatgca ggctggcgag
     4201 ggaccctttc acaagtcccg atagctaccc tcactgagat gaaaaaaaga tttggaatcg
     4261 aaccatcaag agtttcttgc gtggttggtc catctattgg aggttgctgt tatgaggttg
     4321 gtgaggatgt agcgtctcaa tttattcata aattcgataa ttggaaggac tttttattta
     4381 gaaaaaacaa tcttaaatac atccttgatt taaagggagc gaatcgaatt aatctatcta
     4441 aagagggagt gaaggacatt gaggtaataa acatctgtac aaaatgcgat agtgattttc
     4501 attcatatag aagggatggt gaaaatgcgg ggaagcaact gagcttcgca gggcttgttt
     4561 agactgtatt cattgcatgt tatcattact tatagcaggt taaggtttga atttagtagt
     4621 tttcaggagg ctaaaatgtt tcagattatg tgtgtgctga cttcagagga ttattggtat
     4681 ctcgattcga tgaaggagag caacataccc gttaactttt acggctatac atttgaggtt
     4741 gaggggggag gggaggccga aggttttgga agggttaagc tcatggtaat tgagctaata
     4801 aacgctagga tggctgtagg gtttgctatt ccaaggaata tgtctgtaga tggcgaattt
     4861 gaacttcgat ttttatgtca tgaaagtcct acaaacgaaa ttccaattgt ttgcaaactt
     4921 tcccaggaag tgaaacgtac tacgtatagg ggtgatgacg atgcaaagct agaatatatc
     4981 ggtttttcac ttgagaaatt ttatgagagt aagggcgcaa cattctattt ttatgacctt
     5041 aggggaagtc aactggataa acagatgagc aaatgaaatc tttatttgtt tagactacct
     5101 gattttaaaa gtctaaagat ttgcttacac ctttgccttt agctttcttc aaaatga
//
LOCUS       DATDRL010000310         5523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_109285, whole genome shotgun sequence.
ACCESSION   DATDRL010000310 DATDRL010000000
VERSION     DATDRL010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109285"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VI935_10400"
     CDS             complement(<1..548)
                     /locus_tag="VI935_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26045.1"
                     /translation="MAKKGNSTQKNNGYEAALKYPLFDSIFNRRSRRFGLGMELADSS
                     LEFKSSAPPIPLDEVEEALLVWAGTGLTGLCLADLPPETGIDLLCQWTGRTWPSACNN
                     HGTELFFTNDKGLYFVNVKGMLPKDKEVDIFFKMNREEKIKRLLELYRESLVKLEDGR
                     ANLPDKMPGLFDFNQWNTNKPGA"
     gene            complement(842..1078)
                     /gene="tatB"
                     /locus_tag="VI935_10405"
     CDS             complement(842..1078)
                     /gene="tatB"
                     /locus_tag="VI935_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HLE26046.1"
                     /translation="MFGIGTSEILIILLIALIILGPKEIPKIARTLGRTMREFQRVTD
                     ELKHTIDSEIEVDEEETKAKPQENPIEEKKTTDA"
     gene            complement(1464..1844)
                     /locus_tag="VI935_10410"
     CDS             complement(1464..1844)
                     /locus_tag="VI935_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26047.1"
                     /translation="MEYVGKTYIEYLEHGVTRIEGKIDEVLDRELKSVKLPDKAYGFR
                     FFDIIVAQVEVDGRLIDLHSKRINESPVYYYRARVLTHKEVTEGVPNNEKLLLYMNIN
                     GWDKVIQTRTGQFLEFREEDVILD"
     gene            2296..2622
                     /locus_tag="VI935_10415"
     CDS             2296..2622
                     /locus_tag="VI935_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26048.1"
                     /translation="MSCLAGLENPAYCVICIDVGARLAPALFRQPQGLSLQLGNDLPP
                     LVHQQACPEHVEGTGGLTHPVYNVMGRQHVPLTRAPSLRSGLRLWAGSLAADRLVPFT
                     RIPHQP"
     gene            complement(2587..3717)
                     /gene="carA"
                     /locus_tag="VI935_10420"
     CDS             complement(2587..3717)
                     /gene="carA"
                     /locus_tag="VI935_10420"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HLE26049.1"
                     /translation="MKALLVLEDGTVFEGEGFGAIGETYGEVVFNTSITGYQEILTDP
                     SYNGQIVVMTYPEIGNYGINLEDVESIRPFVRGFVVNEYWSVPSNWRSCENLGSYLSR
                     YGVVGIEGIDTRELTRRIRTYGAQRSVISTVDLDQESLLRKVRSSPSIVGNDLATEVS
                     CTEPYRWQTGSEGWSPDYNKQTKLEKHFRVVAYDFGIKRNILRRLVDTGCNVLVVPSR
                     TPPHEVLTMNPDGIVLSNGPGDPAAVSYAIESVRTLIGKKPIFGICLGHQIIGWALGG
                     KTFKLKFGHRGANQPVKNLLTGRVEITSQNHGFAVDADSLGSNVEITHVNLNDNTVEG
                     LRHKEYPLFSVQYHPEASPGPHDSAYLFDEFLRLMGDSGKGY"
     gene            4151..4900
                     /locus_tag="VI935_10425"
     CDS             4151..4900
                     /locus_tag="VI935_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLE26050.1"
                     /translation="MSNRITLIVEALGNTGMDLINPGLYPYRELKPSHRNYWIAVFVN
                     LFFKGYNGAEYIDANNIPEKGPAIIAANHFSHLDGLIINAASIYTRRRAVVFLVAADV
                     YNSNLPFRIMCDLVNCIPVKRDENDRAALLKTIRLLRDGNLFGIFPEGQRSRDGGIGE
                     GKEGAAVIALATGFPVIPVGISGTFEALRRKTKIIKPAKTRLKFGSPLVFEKERHPSA
                     ERVSWVSDHIMNEIKRLHTEALGGEKQKLAA"
     gene            complement(5018..>5523)
                     /locus_tag="VI935_10430"
     CDS             complement(5018..>5523)
                     /locus_tag="VI935_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HLE26051.1"
                     /translation="LPPYDAEILTASKEVAEYFEECLKHYDKPKTVSNWIMTDVLREL
                     KGGEEGIKTFPVTPERLTELIKLIDDGTISGKIAKDVFSEMVSTKKGAGEIVSEKGIK
                     QISDQAEIEALISQVIDKNQDEVSRYRAGQEKLLGFFVGEVMKATQGKANPKLVNEIL
                     RKKLKED"
BASE COUNT         1551 a         1270 c         1069 g         1633 t
ORIGIN      
        1 gcaccaggtt tgtttgtatt ccactggtta aaatcaaata gacccggcat cttgtcaggg
       61 aggttggctc taccatcttc tagctttacc aaactctcgc gatagagctc caggagacgt
      121 ttgatcttct cttcacgatt cattttgaaa aagatatcaa cctctttgtc ttttgggagc
      181 attcctttta catttacaaa ataaagaccc ttatcatttg taaagaatag ctctgttccg
      241 tggttattgc atgctgaggg ccatgtgcga ccagtccatt gacataaaag atcaatacct
      301 gtctcaggag gcaaatccgc taggcaaagc cctgttagcc cggtccctgc ccacacaagt
      361 agtgcttcct ctacctcatc taaaggaata ggcggggctg aggacttaaa ctcaagtgag
      421 ctatcagcaa gctccatgcc gagtccaaag cgacgagaac gacgattaaa aattgaatca
      481 aatagtggat acttaagcgc agcttcatat ccgttattct tttgtgttga attacctttc
      541 ttagccataa tgcttcctcc aaagcgttaa tgaatagcag taaaaaatgt aaaagaagag
      601 atcaaaaaaa tcaaatttat aacccaaatg agattcggtc ggccattgcg aggaatcctt
      661 cgacggagtt tacactgatc gaagtagaag tgctcaggat aaactccgtg gcgaagcaat
      721 tcacgatgaa ttcgtcgggg atcgcttagc tatcgctcgc gataacagtc gaaaccttcc
      781 agcaaagtat aaggaattac catgttacac taaaactctt taatttactg catatcttca
      841 gttaagcatc ggtggtcttc ttttcttcaa tcggattttc ttgaggtttt gcttttgttt
      901 cttcttcatc gacctcaatt tcagaatcta tggtgtgctt aagttcatct gtaactctct
      961 gaaactccct catcgttcgc ccaagtgtcc ttgcaatctt aggtatctcc tttggtccaa
     1021 gaatgataag cgcaatcaaa agaattatta gtatttcact tgttccaatg ccaaacatgt
     1081 tatgcgcctg atgaaattaa actaacatat taaacgaact tctacaaaaa ttcgggtaat
     1141 gattcgaccc ttcgactagg cctgtcgtga gctaagtcga acggcccagg gtttaccaca
     1201 gtgtagacat acgagtttta ctcattgccg agctaaatta tatggaagct atcctccggt
     1261 ttaaccggag gacttcctct acaatttgca gcggaaacct ttaggtttct atcgggatgc
     1321 ggatttcagg atagactgga cattcctgtc cagtcgggcg gggttggaaa accccgccta
     1381 tcgcggcatt cccaaattac aaattagcaa attacaaagg gcaggtcaca agtattgttt
     1441 gtgacttgct gacccctatg tacttaatct agaataacat cctcctctct aaactctaga
     1501 aattgtcctg tgcgtgtttg aatgacttta tcccatccgt ttatattcat atacaataac
     1561 agtttttcgt tattcggaac gccctcagta acctccttat gtgtcaaaac acgagccctg
     1621 tagtaataaa caggcgattc attaatccgc ttactgtgta ggtcaattaa cctgccatca
     1681 acctcgactt gtgccacgat gatatcaaaa aacctaaagc cataagcctt atcaggcagt
     1741 ttaacactct ttaattccct atctagtact tcatcaattt tgccttcgat cctggtgaca
     1801 ccgtgctcta aatactcaat ataagtttta ccaacatatt ccattgtttg atctccattt
     1861 cagctattta aattccccat tctcttcata ttctttttct tagctcagtt tattttatgc
     1921 ttatcttcac ttatttcaaa atctatcttt attattccaa gtgctttacc cctccaaaat
     1981 agattataga acaagctaaa gcgtaaaatc aagaaaaggc ttcccgttgt tcttgtactc
     2041 tctaattcag ctatgatttg ggttcaatct atacgcccac cagcaagcct gtcctgagca
     2101 tgtcgaagga actggtggac taccctggag aatagacaaa cgtaatgcga tggtatcaag
     2161 ctgcttcata ctgggtaggc aacgagtctc gctcgttgcc aaagtaccta ttttatcata
     2221 gtgaagatag acgagacatt ctcgcctcgt tgggctagag aacccaatgc gcaattacag
     2281 gtagacagga cattcatgtc ctgtcttgcg gggttggaaa accccgccta ttgcgttatt
     2341 tgcattgatg taggggcacg tcttgcacct gcccttttca gacaaccgca agggttgtcc
     2401 ttacaattgg ggaacgattt gcctcccctt gtccaccagc aagcctgtcc tgagcatgtc
     2461 gaaggaacag gtgggcttac ccacccagtt tataacgtca tgggaaggca acacgtccca
     2521 ttgacgagag ccccttcact gcgttcaggg cttcggctct gggcaggctc gctcgctgcc
     2581 gaccgcctag taccctttac cagaatcccc catcagcctt aagaattcat caaaaagata
     2641 ggctgagtca tgagggccgg gcgatgcttc gggatggtac tgaactgaga aaaggggata
     2701 ttccttgtgt cttaaaccct caactgtatt gtcgtttaga ttcacgtggg taatttcaac
     2761 attacttcct aaagaatccg catccactgc aaacccatgg ttctgtgatg taatttcaac
     2821 cctacctgta agaagattct tcacaggctg attagctccc ctatgaccaa actttagctt
     2881 gaaggttttg ccacctagag cccagccaat tatttgatgt ccgaggcata ttccaaagat
     2941 cggtttcttc cctatgagcg tccttacact ctcgatcgca taggaaaccg cagcagggtc
     3001 gcccggacca ttagatagaa ctatgccatc tggattcatg gtaagaacct catgaggtgg
     3061 cgttcgagaa ggaacaacca acacattgca tccagtatct acaagtcttc tcaggatatt
     3121 ccgctttatg ccgaagtcgt aggcaacaac cctgaagtgt ttttcgagct ttgtttgctt
     3181 attataatcc gggctccaac cctcacttcc tgtctgccac ctgtagggtt cggtacaact
     3241 cacttccgtg gcgaggtcat ttcctacaat agaaggggat gaacgcacct tccttaacaa
     3301 gctctcttgg tccaaatcaa cagttgatat aacactgcgc tgcgctccat atgtgcggat
     3361 tcttcttgta agctcccttg tatctatacc ttcaataccc actactccat acctagagag
     3421 ataagaacct aaattctcac aagaacgcca gttacttgga acagaccaat attcatttac
     3481 gacaaagcct ctcacaaatg gtcttattga ctcaacgtcc tccagattaa tcccataatt
     3541 tccaatctct ggataagtca taacaacgat ctgaccatta tatgaagggt cggtaagaat
     3601 ctcctggtag cctgtaattg atgtgttgaa cacaacctcg ccgtaggttt ccccaatagc
     3661 tccaaacccc tcgccttcaa atacagttcc atcttctaag acaagaagcg ctttcatatt
     3721 tcggaggtga aaatacatct taaacaaatt gttgtcaaat taatctaaga aggttttcat
     3781 tatgtgattc agtattcaat gaagaacact gtagcaactt aatagtatca tcgtttaatc
     3841 agacaaccct ttagattgct gaaagctggg caaggcatat cgtttgtcat tgcgaacaca
     3901 ttcgcttcgc tcagtgtaaa ctccgtgaag caatccagta tttagattgc ttcgtcccct
     3961 tcgacgtaac tcagggttcc tcgcaatgac atttccacgt tgccataaca atgatataga
     4021 aaactgtaac tagatacagg gctctgtcga tttaaggtaa atcaattgat atacaggttt
     4081 aacttgcttt ttcctccgaa aaatgcacaa ttaatttaaa ttaatacaaa aaggaagtag
     4141 cagatataga atgtcgaata gaattacttt aattgtagaa gcattaggaa atacaggaat
     4201 ggatttaata aatccagggc tctatcccta tagagagctt aaacctagtc ataggaatta
     4261 ttggatagca gtttttgtaa acctattttt taaaggctac aatggagcag agtatattga
     4321 tgctaacaat attcctgaaa agggacctgc gatcatagcc gcaaatcatt tctcgcacct
     4381 tgatggtcta ataattaatg cagcctcaat atacacaaga agaagagccg tcgtgtttct
     4441 cgttgccgct gatgtttata actcaaatct accatttcga ataatgtgtg atcttgtaaa
     4501 ctgcatacct gtaaaaaggg atgaaaatga tagggctgca ctattaaaaa cgattaggct
     4561 cctccgtgat ggaaatcttt ttgggatatt tcccgaaggg cagagatcaa gggacggagg
     4621 tattggagag ggaaaagagg gtgcagcagt aattgcactt gcaacaggct tccctgtaat
     4681 accagttggt ataagcggaa cctttgaggc tctaagaaga aaaacaaaga ttattaaacc
     4741 ggcaaagacg aggcttaagt ttggctctcc acttgtgttt gagaaggaaa gacatccatc
     4801 ggcagaaaga gtatcttggg taagtgatca cattatgaat gagattaaga gactacatac
     4861 cgaagccctt ggtggagaaa aacagaaact tgcagcctaa tcttcatttc gtgattcgtg
     4921 attagtggtt cgagtcacga aacccgagcc acgattcaca agctgtgtgg tttcaattac
     4981 cattgttgaa tattatgcgt tcaactatcg tcggtgttta gtcttcctta agtttttttc
     5041 tcaggatttc gtttactagc ttgggatttg cctttccctg tgtagccttc ataacctcac
     5101 ccacaaaaaa accaagaagt ttttcctgac cagctctgta ccttgacacc tcatcctgat
     5161 ttttatcgat aacctgagag ataagagctt caatctctgc ttgatcacta atctgtttaa
     5221 tccccttttc tgaaacgatc tcccctgccc ccttcttggt tgaaaccatt tcggagaata
     5281 catcctttgc tatcttgcca ctgatggtgc catcgtctat gagttttatt agttcagtta
     5341 gcctttcggg tgtaaccgga aatgttttaa ttccctcctc tcccccttta agctccctca
     5401 aaacgtcggt cattatccag ttacttactg tttttggctt gtcatagtgc tttaagcact
     5461 cttcaaaata ttccgcaacc tctttcgatg ccgtgagaat ttctgcatcg tatgggggaa
     5521 gct
//
LOCUS       DATDRL010000311         2309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3015447, whole genome shotgun sequence.
ACCESSION   DATDRL010000311 DATDRL010000000
VERSION     DATDRL010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3015447"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VI935_10435"
     CDS             complement(<1..358)
                     /locus_tag="VI935_10435"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLE26052.1"
                     /translation="MGISFELGKELEAIKEEVHRFAEKEIRPRLREFEKNEDVPDELR
                     KRFSELGVSAIDYPEEYGGSGLGLIGAVFIIEELAWGDIGTALALINGPGLGGYAILE
                     LGNENQKRKYLSFFSNP"
     gene            complement(395..1096)
                     /locus_tag="VI935_10440"
     CDS             complement(395..1096)
                     /locus_tag="VI935_10440"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HLE26053.1"
                     /translation="MLSSSKKTIFFDFGDTLAATTPSYLIRIAMALSAAGYPVSDRGF
                     ELAYIKTDYEIYARYKAKEEITPDEYREWFFPILCKYLCLEGDPYEIRSKMRLELKSI
                     KFSRAQLPGASELLDFLKGKGYILVVISNNDGKTEEKCEEVGIREYFDIIIDSTNIGL
                     IKPDARIYQFVLEKLKLSNGQAIHIGDLYGSDVMGGLNAGIDVIWLNNRDIQQLDGSK
                     VIEYENLSEIKKLFE"
     gene            1200..>2309
                     /gene="glyA"
                     /locus_tag="VI935_10445"
     CDS             1200..>2309
                     /gene="glyA"
                     /locus_tag="VI935_10445"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HLE26054.1"
                     /translation="MSILEKVDPEIAKAIRLEIERQEWKLELIASENYVSEAVLEAMG
                     SVLTNKYAEGLPGKRYYGGCEFVDIAESLAIERAKKLFGADAVNVQPHSGAQANMAVY
                     FAALKPGDTILGMNLSHGGHLTHGSPVNFSGTLYNVIPYGVREDNNLIDYDQVRELAL
                     KHRPKLIVVGWSAYPRKLDFKRFREIADECGALVMADIAHPAGLVVAGLYPTPIPYCE
                     FVTTTTHKTLRGPRGGMIMMKKDFERVVNSKVFPGTQGGPLMHIIAAKAVAFHEALQP
                     SFIEYQNQIVNNAKILGESLKKRSFKLVSGGTDTHLVLVDLRETELTGKMAEETLEKA
                     GITVNKNAIPFDPRPPAIASGIRIGTPAITTRGMKE"
BASE COUNT          673 a          472 c          458 g          706 t
ORIGIN      
        1 aaggattcga gaaaaatgaa aggtatttcc ttttttgatt ttcattccca agctcgagta
       61 ttgcataccc tccaagccca ggcccattaa ttaaagcaag agcggttcca atatctcccc
      121 aagctagctc ctcgattatg aatactgctc caattaaacc aagtccgctg ccgccatatt
      181 cttcaggata gtcgatggca ctaaccccga gctctgagaa tctttttcta agctcatctg
      241 gtacatcttc atttttttca aattctctca atctgggccg gatttctttt tctgcgaacc
      301 gatgcacttc ttcctttata gcttctagtt ctttgcctaa ttcgaaagag attcccatct
      361 tatttactta tgtttttaaa gggaaagata atatttactc gaagagtttt tttatctctg
      421 agagattttc gtactcgata accttacttc cgtcaagttg ttgaatatca cggttgttta
      481 accatattac atctatacct gcgtttaatc cgcccattac atccgaacca tacaagtctc
      541 ctatgtgtat tgcctgacca ttagataatt tgagtttctc taggacaaat tggtaaatgc
      601 gggcatcagg ttttataagt cctatatttg tcgaatcaat aataatgtca aaatattctc
      661 taattcctac ttcttcgcat ttttcttcag tttttccatc gttatttgat attactacta
      721 atatataacc ttttcccttt aaaaaatcga gaagctcact agcccctgga agttgtgccc
      781 tactgaattt tattgacttt agttctagcc tcatttttga gcggatctca taagggtccc
      841 cttctagaca gagatactta cacagaatcg gaaagaacca ttcacgatat tcatctggag
      901 ttatttcctc cttagcttta tacctcgcat aaatttcata atcagtcttt atataagcta
      961 attcgaaccc cctatctgaa actgggtacc ccgctgcgct tagcgccatt gcaattctaa
     1021 tcaggtaaga aggcgtagtc gcagcgagcg tatcgccaaa atcgaagaaa atcgtttttt
     1081 tggagcttga taacacctgg tttttcctct tattatctat atttatacat gcttttggct
     1141 agcttgccaa ctcaactaat tttagatacc ctttcatgaa ttcattgaga tcataaatta
     1201 tgtctatttt agaaaaggtt gatccagaaa tagcaaaagc aataaggcta gagatagaac
     1261 gtcaagagtg gaagcttgag cttatagctt ctgaaaatta tgtaagcgaa gcagtgctcg
     1321 aagctatggg ctctgtgctc actaataaat atgctgaagg gcttcctgga aaaagatact
     1381 acggcgggtg tgagtttgta gacatcgctg aatctcttgc aattgagagg gcaaaaaagc
     1441 tatttggcgc cgatgcagta aatgttcagc cacactcagg cgcacaggcg aatatggctg
     1501 tatactttgc agccttgaag ccaggggata cgattcttgg tatgaatctt tctcatggcg
     1561 ggcatcttac acatgggagc ccggtgaatt tctccggaac cctatataat gtaattccct
     1621 acggtgtaag agaagacaat aacctaattg attatgatca ggtaagggag cttgccctta
     1681 agcaccgtcc aaaattaata gtagttggat ggagcgcgta ccccagaaag cttgacttta
     1741 aaaggtttcg tgaaatagca gacgagtgcg gagcattagt gatggctgat attgctcacc
     1801 ctgctggatt agttgttgct ggactttatc cgacacctat tccatattgc gagtttgtaa
     1861 ccaccactac acataaaacg ctacggggtc caagaggcgg aatgatcatg atgaaaaaag
     1921 attttgagag agttgtaaac agtaaggttt ttcctggaac tcaaggtgga cctcttatgc
     1981 atataattgc ggcaaaagct gtggcatttc acgaggctct tcagccgagt tttattgagt
     2041 atcaaaatca gattgttaat aatgccaaaa tcctgggaga gtctttaaag aaaaggagtt
     2101 ttaagctagt ttcaggaggc acagatacac atctggtact cgtagatcta agggagacag
     2161 aactcacggg gaaaatggct gaggaaacct tggagaaggc tggtataact gttaataaaa
     2221 atgctattcc atttgatcca agacctcctg caatagcaag cggaataaga attggaacac
     2281 ctgctattac cactagagga atgaaagag
//
LOCUS       DATDRL010000312         2409 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1388467, whole genome shotgun sequence.
ACCESSION   DATDRL010000312 DATDRL010000000
VERSION     DATDRL010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388467"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..250
                     /locus_tag="VI935_10450"
     tRNA            178..250
                     /locus_tag="VI935_10450"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:212..214,aa:Glu,seq:ttc)
     gene            complement(469..741)
                     /locus_tag="VI935_10455"
     CDS             complement(469..741)
                     /locus_tag="VI935_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLE26055.1"
                     /translation="MNWKIEVKPTAEKYYRKLNKPTKKRIKEALRKLEESNNPLFHEN
                     VRALTGELKGDYRLRVGDWRIMFTLDRERKVIYVYAILPRGEAYKD"
     gene            complement(719..964)
                     /locus_tag="VI935_10460"
     CDS             complement(719..964)
                     /locus_tag="VI935_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26056.1"
                     /translation="MRTVNVRIPEELRDTLKIIATVEKRDMKDILTDLVVEYVERHKE
                     TLEILSNPKWVEMVRKGKKEVEEGIKGKSLDELEDRG"
     gene            1387..1725
                     /locus_tag="VI935_10465"
     CDS             1387..1725
                     /locus_tag="VI935_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26057.1"
                     /translation="MADKTFTNPLTLKTDTRITADRILECIEILRFQGLVPHQIKFGM
                     VDKCPECGEPTWGLFMAEDEGVEIEKGEPLGICLQCNATIFVDEDGKPNARIISSQDL
                     LRGLRIGKGK"
     gene            complement(1965..2168)
                     /locus_tag="VI935_10470"
     CDS             complement(1965..2168)
                     /locus_tag="VI935_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26058.1"
                     /translation="MHKAIIVLLLTSLFCATGRSEGIDIKNLMINHPEWYIKITDWHV
                     YAGWSCPAIIHHVTIENTSDIAY"
     gene            complement(2182..>2409)
                     /locus_tag="VI935_10475"
     CDS             complement(2182..>2409)
                     /locus_tag="VI935_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26059.1"
                     /translation="AGKGAWRPCNRLGGKGYVKFDLVENKRRNKYKAACNAKKNSNSI
                     KKSYLINESNHKAARIILVVLGQHSILVKIK"
BASE COUNT          710 a          491 c          401 g          807 t
ORIGIN      
        1 agctcatagt cttctttaat tctttttcct ctaagaacgt cagggaaaaa tcggttatta
       61 agttgtgagg tgtccaagga ggtgtccata tggtgtctct ttttacccat ttgttacagt
      121 tggtatgtgg gaagaaaagt atcataactg attgaagtta caaggattct atactaagtc
      181 atacaaagtg gaactaatac cgtattgccc tttcaaggcg gtaacagggg ttcaaatccc
      241 ctcggggacg ctcaattctt ttctttaatt tctgctaatt acgagacggt aaccctattg
      301 ttacctttct atgtcagtga gtttgtcagt aactctcttt tgattgttcg gggctatatt
      361 ccttcgctta gctttctccg tttacaaata ttaatttcct cgacgataat tccacttagt
      421 ttttatatcc atagaacctt gtaaaatgtt ttactgactc ccttgcgatt agtctttata
      481 tgcttcacct ctaggcaata ttgcatatac gtaaattact tttctttccc tatccagtgt
      541 aaacatgatt ctccagtccc cgactctgag acggtaatcc ccctttagtt caccggttaa
      601 agccctgaca ttctcatgaa ataagggatt attgctctct tccaatttcc gaagagcttc
      661 ttttatcctc ttctttgtag gtttattcag ttttcggtaa tacttctccg cagtgggttt
      721 aacctcgatc ttccaattca tctagcgatt ttccttttat tccctcttca acttccttct
      781 tgcctttcct aaccatctct acccatttag gattagaaag aatttctaga gtctctttat
      841 gccgctctac gtattcaact acaagatcag taagaatgtc tttcatatca cgcttctcta
      901 ctgttgcaat aatctttagc gtatccctta actcttcggg aatacgaaca tttacagttc
      961 tcatgacttt ttctccttta gtatactagt atataataat gctaggatac tgctaattca
     1021 gattatttca atattacacc gaaaaagtta acagtttagc gtataaggtc agaaaaatca
     1081 cagtcgaacc actaataaac agaactctag acttaattag tcgaatatcc ctccctgttt
     1141 atccaacaag gcgattaaca aaataaaaga ggaagcctta gcgcgataaa gagattgctt
     1201 cttgtgcctg gaactttaat tttatagggg ataaggacta aagtaatatg gcatctccaa
     1261 gaaccagatt tttgtctgac ttaaaaatta agcctgcgga atcctttagg attccattca
     1321 taaattttaa agtaatcaca aaaggaaaaa atattgtata atcaagaaaa aataatggag
     1381 ataaacatgg cagataaaac ctttacaaac ccattaactc taaaaacaga tacacggatt
     1441 acagcagaca ggattttgga atgtatcgaa atacttagat ttcaaggctt agtaccccat
     1501 caaatcaaat ttggaatggt agataaatgc cccgaatgcg gagaaccaac ctgggggcta
     1561 tttatggctg aagacgaagg agtcgaaata gagaaaggag aaccccttgg gatttgtctt
     1621 caatgcaacg ccactatatt tgttgatgaa gatggtaaac caaatgcgag gattattagt
     1681 agccaagatt tactaagggg tttacgaatc ggcaagggga aatgatagat tccattatct
     1741 tcaatattat cttccacctg atacaggagt agcccctaag acctctaaat acccggcata
     1801 catagccata catcctgctc ctagagtggc gcgcgatcca tttttagata agtttcttta
     1861 ctatgaggca gcacaaccac aggtaatact cctgtttcct ggcttatttg cgttccgtag
     1921 ttttcgtatg ttgtcgaata atatagtact cttatttaaa tgtcttaata ggctatatca
     1981 cttgtgtttt caatagttac atgatggatt atggctgggc agctccatcc cgcataaaca
     2041 tgccaatccg taattttaat ataccactcc ggatgattaa tcataaggtt tttaatatct
     2101 attccctcag atcttcctgt agcacaaaat agactagtca gtaataagac tattatcgct
     2161 ttatgcatat atacctcctt cttatttaat tttaactaat atggaatgct gtcccaatac
     2221 aaccaatata atacgggctg ctttatggtt actttcgtta atcaaataag atttttttat
     2281 tgagttagaa tttttcttag cattacatgc agctttgtat ttattccgtc tcttattttc
     2341 aactaaatca aatttgacat aacctttgcc tcccagtcgg ttgcaaggtc tccaagcgcc
     2401 tttgccagc
//
LOCUS       DATDRL010000313         2184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3277338, whole genome shotgun sequence.
ACCESSION   DATDRL010000313 DATDRL010000000
VERSION     DATDRL010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3277338"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2184
                     /locus_tag="VI935_10480"
     CDS             <1..>2184
                     /locus_tag="VI935_10480"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HLE26060.1"
                     /translation="EKAKLAIELEKISPGLGEDTLNALLLLSDLTTNDKELGVSKFLT
                     EAHINEVTSKLESIDLSPAIPGEKIKTYWRPLAAILVLSTTMLILAPKGFQSLLFSAN
                     VLPPSEPNLLELADIEVEYTYPAYTKLSSQVVKGSTGDVKALKGTHVTFKAIPFKKLV
                     KGELVIENGLVTPISSDGERINGEFTILSNGSFFIQDKNGKQRSRILKITAEDDKTPK
                     VSIDSPSDKVIEIEDEENLDIFYKADDDFGLKKLLLTFKTQRGESSRSIGQIKEEPKF
                     LEGKFTWDFSGVESEPEEIIEALIYAYDNNTVSGPKVGVSNVIKIKLNDPRKRNKDIL
                     LSVENLLDQFLDTLADEIENRPGDNKNINRTKVIQYGISSKIEKVIDTLNEILESVKD
                     DDFSDYTYFLGLSNMKIGTEDLLNERHDLLSSLSLSHISRLAGLITKEINEFEDNILF
                     LDSLLEGDKLRDSLLYGRETLSKYNELSELVKNLKQNENEKTRKEVEKKIEEIRGLIS
                     QLTKTLGSINLDIPDGYLNPDAFNSLDLEGKLDELMNLIKEGHIDKALKMLASLGENL
                     QEMVASLESGFQSYSSASLSKEITELNNIISKIEGLEKDEKSLKEKTEELKSSLLKDP
                     SSKGNLNKFVEREKKKIEMLKNLLIETRSKLFPNFEKNELVEGSILVDRILYEIDELK
                     HWLESFEFNEALLQAKDVEEQTIGLRNLSDLRLGKIASASLEVENS"
BASE COUNT          804 a          381 c          426 g          573 t
ORIGIN      
        1 gagaaagcca agctcgctat agaacttgag aaaatatccc ctggtctcgg agaggatact
       61 ctaaacgcat tactcctatt atcagattta accacgaatg ataaagaact aggtgtatca
      121 aaatttctca cagaagccca tattaatgaa gttacctcca aactcgaatc aatagattta
      181 tcccctgcta tacccggtga gaaaataaaa acttactgga ggcctctcgc tgctatcctt
      241 gttttatcta caactatgtt gatacttgca ccaaaaggat ttcagagttt attattcagc
      301 gctaatgtcc taccaccctc agagccaaat ctccttgagc ttgcagatat tgaagtcgag
      361 tatacatacc cagcttacac aaaactatcc tcccaagtcg taaagggaag tacaggagat
      421 gtaaaggctt taaaaggaac tcatgtgacc tttaaggcaa tcccgtttaa aaaattggtc
      481 aagggagagc ttgtcatcga aaatggtctt gtcactccta tcagttcaga cggagaaaga
      541 atcaatggtg agtttacaat tctttcaaat ggaagttttt ttattcaaga caagaatggg
      601 aaacaaagat caaggatcct taaaattact gctgaagatg ataaaactcc gaaagtctcc
      661 attgactctc caagtgataa ggtcatagag attgaggatg aggaaaatct agatattttc
      721 tataaggcag atgacgattt cggacttaag aaactactct taacatttaa aacacagagg
      781 ggggagtcaa gtaggtccat tggacaaata aaagaagaac caaaattcct agaaggcaaa
      841 ttcacatggg attttagcgg ggttgaatca gaacctgaag agattataga ggctctgatt
      901 tatgcttacg acaataacac agtctcagga ccgaaggtcg gggtttcaaa cgtgataaaa
      961 ataaagctaa atgatccaag gaaaaggaac aaggatatac tattatctgt agagaatttg
     1021 cttgatcagt tcttagatac acttgcagat gagattgaga accgccctgg ggataataaa
     1081 aatataaaca gaacaaaagt cattcagtat ggaatatcta gcaagattga aaaggtcatt
     1141 gatactttga acgaaatact agaaagtgtt aaagatgacg acttttcgga ttatacatat
     1201 ttcttagggc tttcaaatat gaaaatagga acagaggatt tactcaatga acgacatgat
     1261 ttgctctcat cattgtcgtt aagtcacatt tcccgactgg caggcttaat aacaaaagaa
     1321 attaacgaat tcgaagataa catacttttt ctcgattcct tactcgaggg agataaacta
     1381 agggattcac ttctttatgg aagagaaaca cttagtaaat ataatgagct ttcagaactt
     1441 gttaagaatc ttaaacagaa tgagaacgaa aaaaccagaa aagaagtaga aaaaaagata
     1501 gaagagatac gtggtctaat atcacagttg accaaaacgc tcggctcaat caatttagat
     1561 attcccgatg ggtatctcaa tcccgatgct tttaattcct tagatttaga aggaaagcta
     1621 gatgagctta tgaaccttat taaggagggt catatagata aagccctcaa gatgcttgct
     1681 agcttaggag aaaaccttca agagatggta gcttctctcg aaagcgggtt tcagtcatat
     1741 agctctgctt cactttctaa agagattaca gagctaaata atattatttc aaagattgag
     1801 ggcttagaaa aggatgaaaa atcgctaaaa gaaaaaacag aagaacttaa aagctctctt
     1861 ctaaaagacc catctagcaa gggaaaccta aataaatttg tagaaagaga aaagaagaaa
     1921 atagaaatgc taaaaaatct tctcatagaa acccgctcta agctcttccc caattttgaa
     1981 aagaacgaac ttgtagaagg ctccatctta gttgacagaa tcctttacga aatcgatgaa
     2041 ttgaagcact ggcttgagtc atttgagttt aacgaagcct tacttcaggc aaaggacgta
     2101 gaggagcaaa cgattggact aagaaacctt agtgatttaa gattaggtaa aatcgcaagc
     2161 gcctcacttg aagttgaaaa ctca
//
LOCUS       DATDRL010000314         2945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_940035, whole genome shotgun sequence.
ACCESSION   DATDRL010000314 DATDRL010000000
VERSION     DATDRL010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940035"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VI935_10485"
     CDS             complement(<1..639)
                     /locus_tag="VI935_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009768085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HLE26061.1"
                     /translation="MPQKYPKEFLDLCKTVTAKRPKTVIDHILKHGHITTEELKEKYG
                     YNHPPRAVRDAREHGIPLETFRVTGSDGRKIAAYRFGDVSKKRFRKLSGRTGLSKKIK
                     EYLIEKYGCKCFIYLEDMDEGELQIDHRVPYEVGGDSESVEQNPDDFMLLSGSANRAK
                     SWSCEHCENWQTLKKKEVCLSCYWAYPEEYFHVAMRQVRRLDLIWQGKEVEQY"
     gene            complement(608..1555)
                     /locus_tag="VI935_10490"
     CDS             complement(608..1555)
                     /locus_tag="VI935_10490"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HLE26062.1"
                     /translation="MKLPHPIQYQGSKRSLASIILRYFPNKFPRLVEPFSGSAAISIA
                     CAARGKANSYWINDLNKPLAELLGLIINHPVEIANYYETIWNQQHDDPIEHYYRVRED
                     FNRTGDPRLFLYLLARCAKGSVRYNTEGLFNQSPDKRRHGTRPETMRDNIFGVSTLLK
                     GKSVISSLDYKDVLSNVNEDDVVYMDPPYQGVCGDRDSRYFSGISYEEFLAALSELNV
                     RQIRYAVSYDGRTGNKTFGNRLPESLSLTLIELEAGRSSQATLLGRDAITVESLYLSK
                     RLAIEVKASPYFHRKRETKQLVLMETRRRYAAKISKRIP"
     gene            complement(1677..2318)
                     /locus_tag="VI935_10495"
     CDS             complement(1677..2318)
                     /locus_tag="VI935_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26063.1"
                     /translation="MSEARLMGCIPDYTVCIIIVRTMKEKINKGIHITKLDAALRQME
                     TATVLWFNNADPVSIHTLICAAYQILYDLNKHQNVFLMIPDSPMIKPEKQKEWKKILK
                     AASNFMKHAENDPVGTLSFNPEINEFLLFDAAMSYSIITKETRPILQCFLMWLLNFRP
                     EFFLPKYIKRLNQIVPVNVFKNMNRAAFFQYALPLITSNIVTGHKAHDENRRF"
     gene            complement(2481..>2945)
                     /locus_tag="VI935_10500"
     CDS             complement(2481..>2945)
                     /locus_tag="VI935_10500"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HLE26064.1"
                     /translation="YTSMGRLLVMDGKIPEEDLTLSTLITYFDQHPTELDSYLKRNDR
                     YIFFKRVNHAIPYGSIGVPVTPMRSIATDKKVFPAGGLVFAVIEPKKAKKWGWIQNKG
                     KGGKSFFALDQDTGSAIQTPARADIYFGIGDQAMHEAGELNAYSRLYYLLRR"
BASE COUNT          807 a          620 c          599 g          919 t
ORIGIN      
        1 gtattgttct acttccttgc cttgccatat caaatcaagc cgtctaacct gtcgcattgc
       61 aacatgaaag tactcttcgg gatatgccca gtaacaagaa agacaaactt ccttcttttt
      121 cagtgtctgc caattttcgc agtgttcgca tgaccaagac tttgcacgat ttgcagaacc
      181 ggaaagcaac atgaaatcat ctggattttg ttcgacgctt tcactgtcac caccaacctc
      241 gtaaggcacg cggtggtcta tctgaagctc gccttcatcc atatcttcaa gatagataaa
      301 gcatttacaa ccatatttct cgatgagata ttctttaatt ttcttggaaa gaccagttcg
      361 cccggacaac ttcctgaacc ttttcttact tacatctcca aagcgatatg ccgcgatttt
      421 cctaccgtca gaacctgtta cacgaaaagt ctcaagtgga ataccatgct ctcgcgcatc
      481 tcgaaccgct cttggaggat ggttataccc gtatttttct ttcaattctt ctgttgtgat
      541 atgcccatgc tttaaaatat ggtcaataac cgtcttggga cgtttcgcgg taacagtctt
      601 gcaaagatca aggaattctt ttggatattt ttgcggcata acgtcttctg gtctccatca
      661 gaacaagttg ttttgtttcc cgttttctgt ggaaatacgg tgaagcttta acctcgatgg
      721 ctaagcgttt tgataaatat aaagattcaa cggtaatagc atcgcgtcca agcaaagttg
      781 cctgagagga gcggcctgct tctaactcaa ttaaggtaag tgaaaggctc tcaggtaggc
      841 gattgccaaa ggtcttattg cctgtcctcc catcgtagct gactgcataa cggatctgac
      901 ggacattcaa ttctgataat gctgctaaaa actcctcata agatatacct gaaaaatagc
      961 gcgaatctct atcgccgcat actccctgat aaggagggtc catataaaca acatcatcct
     1021 cattgacatt tgatagaaca tctttgtagt caagtgatga tattacagat ttccctttca
     1081 atagggtaga aacaccaaaa atattatctc tcattgtttc gggacgtgta ccgtgacgcc
     1141 tcttgtcggg gctctgatta aacagtcctt cagtattgta tcgcacggaa ccttttgcac
     1201 aacgcgcaag caaataaagg aataatctgg ggtctcctgt tcggttgaaa tcttccctaa
     1261 cacgataata atgttctatt ggatcatcat gctgttgatt ccaaattgtt tcgtaataat
     1321 tggctatttc tacagggtgg ttaataatta agccaagcaa ttcagcaagg ggcttgttta
     1381 agtcatttat ccaatatgaa ttagcctttc ctcttgcagc gcatgctatt gaaattgccg
     1441 ctgagcctga aaagggttct acaagccttg ggaatttatt aggaaagtag cggagaatga
     1501 ttgatgcaag gcttcttttg cttccctgat attgtattgg atgcggtagt ttcatgttat
     1561 aaattccatt ttgccgtttt tatattatac acctaatgtg gagcataacc cgcaggggtt
     1621 aactccatat ttaaaaatca aaacagcgtt aacccctgtc ggtgttgatg cgattgttag
     1681 aaccttctat tttcatcgtg tgctttatgc ccggtgacaa tattactagt gatgagtgga
     1741 agcgcatatt ggaagaatgc agccctattc atatttttga aaacattgac tgggacaatc
     1801 tggttgagtc tcttaatata ctttggcaga aagaattcag gcctgaaatt aaggagccac
     1861 atgagaaaac actgcagaat aggacgggtt tccttggtga tgattgaata cgacattgcc
     1921 gcgtcgaaaa gaagaaattc attaatttct ggattaaaag ataatgttcc aacggggtca
     1981 ttttcggcat gtttcatgaa atttgaggca gcttttaata tttttttcca ttctttttgt
     2041 ttttctggtt tgatcatggg cgaatctgga atcataagga atacattctg atgtttatta
     2101 agatcataaa gtatttggta tgcagcacaa ataagcgtat ggatagaaac aggatcagcg
     2161 ttattaaacc aaagcacagt agcagtttcc atttggcgca gagccgcatc aagttttgta
     2221 atatgaatgc ctttattaat tttctctttc atagttctaa caatgattat acagactgta
     2281 taatctggaa tacaacccat caaccgggct tcggacaccg tgacctcctt tgctcaagac
     2341 atgggtgtag atcatcgtca tgctaacgtc tttggcgaag acaatcagag attatcattt
     2401 accgttttgc aatgtcaagc ttcttttaca tggagagatg acaggaaatt gttttttctt
     2461 taaaaaggat gaagaaggaa tcaccgcctc aacaggtagt agagtctgct ataggcattt
     2521 aattcgcccg cttcgtgcat ggcctggtcg cctattccaa aatatatatc ggctctggct
     2581 ggagtttgaa ttgcgcttcc cgtatcctgg tctagagcga agaaggattt tccacccttt
     2641 cctttgttct gaatccaccc ccattttttt gctttcttcg gttctatgac agcgaagact
     2701 aatccacctg ctgggaaaac ctttttatct gtggcaatac tcctcatagg ggttaccggc
     2761 acgccgatcg acccataagg gatcgcatga tttaccctct tgaaaaagat atagcggtcg
     2821 tttctcttca ggtacgaatc taattcggtg ggatgctggt cgaaataagt tatcagcgta
     2881 gaaagggtca aatcctcttc cggaatcttt ccgtccataa ccaggagacg tcccatactg
     2941 gtgta
//
LOCUS       DATDRL010000315         6156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1485944, whole genome shotgun sequence.
ACCESSION   DATDRL010000315 DATDRL010000000
VERSION     DATDRL010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485944"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1336)
                     /locus_tag="VI935_10505"
     CDS             complement(179..1336)
                     /locus_tag="VI935_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLE26065.1"
                     /translation="MPIVDILLSLIIILVSAKVLGDLAERVAQPGVLGELIAGVIIGE
                     SVLGLIHHEPTLEFLAEVGVILLLFEIGLETEIKEFIKVGIDSLLVAIAGIALPFAFG
                     YAAMRAFGVDSTVSIFAGATLTATSIGITAKVLADLGRLDCLESKIIIGAAVIDDVLG
                     LVILSLVTVLAETGSVSIEIVGVGLIKAIGFLVAAIVIGNILAPQLIKLVDRIRGRGV
                     LFIAAFVFAIVLSYLADKAGLALIIGAFAAGTVLAGTHRKKVIEENLKPLVDVFTPVF
                     FVMVGVSVNLSVLNPFNPGNHRILLIIFVLTAVAVIGKLASGYLATRKGINRLAIGLG
                     MLPRGEVGLIFAQLGKSTGLFSDEVFSALVSTVVLTTFLAPPLLSLVFRKS"
     gene            complement(2035..3027)
                     /locus_tag="VI935_10510"
     CDS             complement(2035..3027)
                     /locus_tag="VI935_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HLE26066.1"
                     /translation="MSNQILWWIVFNIFVLAMLTLDLGIFHRKAHEVKVKEALVWSFV
                     WIGLALLFNVGVYFWQGPQTALEFLTGYLIEKSLSVDNIFVFLLIFSYFRVPALYQHK
                     VLFWGILGALIMRAIFIVAGVALIQKFHWMIYIFGGLLILTGIKMALQKDMEIHPERN
                     PVLRLFQRFIPVTKDYEDDKFFFKRSGRYFATPLFITLLIVETTDLIFAVDSIPAILA
                     ITTDPFIVYTSNVFAILGLRALYFALAGIMQLFHYLHYGLSAILVFVGIKMLLADLYK
                     IPVGISLGVVAGFLLISVIASIIRPKQIESVSAAVNPPIEETKLKSIYQAKDKQ"
     gene            complement(3169..3342)
                     /locus_tag="VI935_10515"
                     /pseudo
     CDS             complement(3169..3342)
                     /locus_tag="VI935_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009543817.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(3609..4466)
                     /gene="htpX"
                     /locus_tag="VI935_10520"
     CDS             complement(3609..4466)
                     /gene="htpX"
                     /locus_tag="VI935_10520"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HLE26067.1"
                     /translation="MNTIKTLLLLAVLSAILIWAGGAIGGKNGALVALIIAGVMNFIS
                     FWWSDKIVLAMYRAREVKPEDAPALYGAVEELAQNAGFPMPKVYVIPEETPNAFATGR
                     SPNHAAVAVTQGIMRLLNTEELKGVLAHELSHIKNRDILIGTIAATIAGAISYLAYMA
                     QWAAIFGGRERDNRGNFLMLLVMIIVAPLAALIIRMAISRAREYGADAGGAKICGNPL
                     YLASALRKLGYVSGKIPLRVNEQTAEATAHMFIVNPLSGRGFATLFSTHPLIEERIAR
                     LEDMAAGRR"
     gene            complement(4533..5120)
                     /locus_tag="VI935_10525"
     CDS             complement(4533..5120)
                     /locus_tag="VI935_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26068.1"
                     /translation="MEIPRALQIARREWGNIRISLQECSDIGEFDSNNHVNSKTTLIS
                     RGNLLVRNLIEMENKLPTHGYFTPEASYVFTTLASRASERLELGGRLAQTFGSGYSWV
                     RTGWLDLSGMEEQKHLIKQLFFFKIFFPLGGYFNWDFNSPLVKTKLKAVFYIFATWQG
                     NPSSYIQDVKDYRSGLEPLWHGLSLALDFPKKFPE"
     gene            complement(5880..>6156)
                     /locus_tag="VI935_10530"
     CDS             complement(5880..>6156)
                     /locus_tag="VI935_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26069.1"
                     /translation="CECKEADYVNNVSPFFYTASYLIASAIETELSLYLMEKYDEEWW
                     RTSEAGDFILKLWKEGGRITSKEILKRVGFEELSFTPLLRSFQEVFG"
BASE COUNT         1892 a         1362 c         1091 g         1811 t
ORIGIN      
        1 tgtcatctat gcctcctttt ttaattaata aacactggtt atcgaagcga aattcaattt
       61 atttgacata taccagcttg ggtttcttta tataacatca caattatcat aagatgccct
      121 ttgagaaccc ctaaattcgg actgacttaa attttattat cattatttat ccataaaatt
      181 aagactttcg aaatacaagc gagagcagtg gaggagctag aaaagtagtt agcactaccg
      241 tagaaactaa tgcagaaaaa acctcatccg aaaaaagccc tgtgctcttt cctaactggg
      301 caaatattag ccctacctct ccccttggaa gcatccctaa accaatagca agccgattta
      361 ttcctttcct agtcgcaagg taaccgcttg ctaatttccc tataacagct acagcagtaa
      421 gcacaaaaat aataagtaga attctatggt tccctggatt aaatgggtta agtacactaa
      481 ggtttacaga tacacctacc attacaaaaa atacaggggt aaacacatct actagcggct
      541 ttagattctc ttctataact tttttcctat gtgtgccagc tagaactgta ccagccgcaa
      601 aagcccctat tattaaagcc aaccctgctt tatcggctag atatgaaagc actatagcaa
      661 agacaaaagc agctatgaaa agaacccctc ttccacgtat acgatcaact agcttaatta
      721 gctgtggggc taggatgtta cctataacta tagctgctac aagaaatcca atagccttta
      781 ttaatccgac tcctactatt tcaatagaaa cagagccggt ctctgctaat acggttacaa
      841 gagataagat tactaaacct aaaacatcat ctataacagc tgctccaatt attatcttag
      901 attccaaaca atcgagcctt ccaagatccg caagtacttt agcggtgatt cctatacttg
      961 ttgcggttaa ggtcgctcct gcaaatatcg aaactgtact atctacccca aaggccctca
     1021 tagctgcata accaaatgcg aatggtagtg ctatccctgc tattgcgaca aggagcgaat
     1081 caatccctac tttaataaac tctttgattt ctgtctctaa cccgatttcg aacaggagaa
     1141 ggataacacc tacctcagcc aggaactcaa gcgtgggctc gtggtgtatt agtccaagta
     1201 cgctctcccc tatgattact ccagctatga gttctcctaa aaccccgggc tgagcaaccc
     1261 tttcagcaag gtctcctagt acctttgctg aaaccaggat aattatcaaa cttaacagta
     1321 tatcaactat aggcatcttg tcttacttca gatggaacct acattttttt ctaaccctcg
     1381 attccccaag ttgttagaac ctttctccct tagcaaacat ttttaaacta aatcctccta
     1441 agctaaagtt gggtaagctt attatatagt tagattttac taacataaga tatttccctt
     1501 aaattaggtc tgctagatgt tcatctgggt ttatcgactt agtctttaac tttctcatat
     1561 acacatccta acatttagaa tctatttgtt gaattggtat aacatagtta tcctcctttt
     1621 gggttagatt cttttttcac ttaattccct atacaacaag aagagctttt tatatagaac
     1681 tcacgttaag tgacgttgat taaacaagct cgtcatttta acttttttac tagtagtgcc
     1741 acagctccgg taaaagcgat aattaccaaa acttgtataa agagcacttt taagtcaagt
     1801 gagtaataag taactttagg tggtgtaaac aagaagttgt attgcctagt caatccatat
     1861 ccatcataat ggatactcca tggtggaaaa atacaagcta gaaaaatcaa gatacaacct
     1921 gcaagaatta tcattctcat agtattctcc tccggtttac taaaacctta tccattagtc
     1981 ggatgagaac tatgcaattt cctaaggatt taggtttcaa tacaagccat tggctcattg
     2041 tttatccttg gcctggtata tggattttaa ctttgtttct tctattggtg gattgactgc
     2101 cgctgaaaca ctctctatct gcttcggtcg aataatcgag gctatcacag agataaggag
     2161 aaaaccagct accacaccta aagaaatgcc aaccggaatc ttatagaggt cggcaagcag
     2221 catcttgatt cccacaaaga ctagaatggc agaaagtcca taatgaagat agtgaaacag
     2281 ttgcataatc ccagccagtg caaagtaaag tgctcgtaat cccaagatag caaatacgtt
     2341 ggaagtgtac acaataaatg gatcggtcgt gatggcaagg atggctggta tagagtcaac
     2401 cgcaaagatt aggtcggttg tttccacaat cagcaacgta atgaacaatg gcgtggcaaa
     2461 atagcgcccg cttcgcttga agaaaaactt atcgtcttcg taatcttttg taaccggaat
     2521 gaatcgctgg aagagcctaa ggactggatt cctttctgga tggatttcca tgtctttctg
     2581 caaagccatt ttgatgccgg tcagaatcaa caaacccccg aagatataga tcatccagtg
     2641 gaacttctgg ataagcgcca caccggcgac aataaaaatc gctctcataa tcaatgcccc
     2701 gagaataccc caaaaaagca ccttatgctg atacaaagct ggcacacgaa agtaagagaa
     2761 gatcaagaga aaaacgaaga tattgtcaac gctcagcgac ttctcgatta gatatccggt
     2821 taggaactcc agagccgtct gtgggccttg ccagaagtaa actcccacat tgaaaagaag
     2881 tgctagacca atccagacaa aactccatac aagcgcttct ttgaccttga cctcatgtgc
     2941 tttgcgatga aaaatcccaa ggtctagcgt taacatcgca agcacgaaaa tgttgaagac
     3001 tatccaccat aagatctgat tcgacaccgg aattcctcct gtttacactg tgtcttttgt
     3061 tagtactaat ttcatctaag tttcttgcaa aactcaattt ccccgatttt agcttaataa
     3121 aagcagtgac ttactacaag gctcattttt agtttgcaag agctccactg cacggagagg
     3181 agacgcaata tcgctgggag acagcctacc agttgccacc tgataaagca ccgactggaa
     3241 gagatgaaaa ttgcgtttat cgtttaaagt cgccttgatt ggggtatatc ctaatgcctg
     3301 tgcagcatat aatccaccaa acccaccaac aatgattacc acatgatggg aacggtagtt
     3361 ttttacttat catgatcttt ttcctactcc taaataaaat cacacttttt cgagaaagcc
     3421 tatcccctac caattaaacc gaaacccgat agttcgaaag ttctgccaag cgactttgcc
     3481 gtctggtaca aatacccctt gttggattta ctatgcagct cagtgccctt aaaagttgac
     3541 tgttcctttt tgccaaccag tttgaagtcc cttcaagaaa catacgatcg gaataccaag
     3601 accaattttt acctccttcc cgctgccata tcttcgagtc ttgcaattct ttcttcaatc
     3661 agtggatgtg tactaaaaag agttgcaaaa ccccttccac taagtggatt tacgatgaac
     3721 atatgtgctg tggcctctgc tgtctgttca tttactctta gcggaatttt tcctgataca
     3781 taacccagtt tcctaagcgc gctggcaaga taaagagggt ttccacaaat ttttgcccct
     3841 cctgcatctg caccgtattc tctcgcacgt gatattgcca tacgtataat gagggcagct
     3901 aaaggcgcaa cgataatcat cacgagaagc atcaggaaat ttcctctatt atctctttct
     3961 cttccgccaa atatagcggc ccactgcgcc atataagcaa ggtaacttat agctcctgca
     4021 attgtagccg caatagttcc aatcagaatg tcacggttct ttatgtgtga aagctcatga
     4081 gcaagaacac cttttagctc ttccgtattc agtaatctca taattccctg cgtcacagca
     4141 acggcagcat ggttaggact tcttccagtg gcaaacgcat tcggggtctc ctcaggaatt
     4201 acataaacct taggcatggg aaaccccgca ttctgggcta attcctccac agcaccatat
     4261 aacgcgggtg cgtcttctgg tttgacttct cttgctctat acatagcaag tacgattttg
     4321 tcgctccacc agaagcttat aaagttcatt accccggcga ttatcaaagc gacgagggca
     4381 ccgtttttcc ctcctattgc acccccagcc cagatgagaa tcgcagagag tacggctaga
     4441 agtaaaagag tttttattgt attcatcgct taacctcctt tctttggcaa attaaacaga
     4501 tttggaagct acccgaggta ccttgggagg tattattcag ggaatttctt aggaaaatct
     4561 aaggctaatg ataaaccgtg ccaaagaggt tctagtccag atctgtagtc cttaacgtct
     4621 tgaatgtaac tactgggatt accttgccat gtggcaaaga tataaaatac agctttcagc
     4681 ttcgtcttta ccaaaggtga attaaaatcc cagttgaaat atcccccaag tggaaagaag
     4741 attttaaaga agaacaactg ctttattaag tgtttttgtt cctccatacc acttaggtca
     4801 agccaacctg ttcgaaccca gctatatcca ctaccaaagg tctgagctag tctacctcca
     4861 agttcgagcc tctctgaagc ccttgatgca agagtagtga agacataaga ggcttctggt
     4921 gtaaaatacc catgagttgg aagtttattc tccatctcaa taaggtttct aaccagaagg
     4981 ttacccctag atataagggt agtcttacta tttacatgat tattagaatc gaattctcct
     5041 atatcactgc actcttgaag ggaaatcctg atatttcccc attctcttct tgcaatttgt
     5101 agtgctcttg gtatttccat tttcttcact ccttttttaa cgtatttttt aagcgatctt
     5161 actaattgcc ttttaggtcc ttaaatgcaa tagccgatag tgcatattgg cttgatgcgg
     5221 accgtatttc ttaaacgaat aagatacgaa accctaatta gaatttgtgt gtaggctaga
     5281 gtatgcttaa atcaggaaaa taaaggaggg gaccttgctg aaatagggct tatataatct
     5341 aaagaataat aaaattcgtc actagaagtt aaaatctctc gattttcgaa taacctagat
     5401 aagagtccaa cattacttcg aatcaaaaaa ctagtttttg aatgaaaatt atttttcccc
     5461 ctagtagccg ttttccttgg agtttttacc tccaggttaa actttgagtt cgaaagaggt
     5521 ttctttttat tcgtattatt cgtaaaagac aattcagatg ccgaatcaac agagagatac
     5581 tgtcctaagt aaaccgcgtt tccctcggcg ttgagagaaa acatcactac aataattgct
     5641 agggcaggga taaaaaactt aaacctgcga agtgtaagta taagtacttg gacgcaaagt
     5701 agcgtaatag tatttttaaa aagaaaattc atatttcctt tctccagaac aagtaagcaa
     5761 actgagtttg tctgcggatt aattacaaag ttattttata ttaacatatg atgctcaaaa
     5821 agacaagaaa ttttttgttg tttgacatta tcactttgca actacaaaag gtataggacc
     5881 tatccaaaaa cctcttgaaa agatcggaga agcggtgtga aactaagctc ttcaaaccca
     5941 actcgtttta aaatttcttt ggatgtaatt cttccaccct ctttccagag tttgaggatg
     6001 aaatcacccg cttcacttgt tctccaccac tcttcatcat atttctccat taggtacaaa
     6061 cttagttctg tctcgatagc acttgcaatt agatagcttg cagtataaaa aaatggagaa
     6121 acattattca catagtctgc ttctttacat tcgcaa
//
LOCUS       DATDRL010000316         7117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2488320, whole genome shotgun sequence.
ACCESSION   DATDRL010000316 DATDRL010000000
VERSION     DATDRL010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2488320"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VI935_10535"
     CDS             <1..235
                     /locus_tag="VI935_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26070.1"
                     /translation="YLHPEYQEVQETFNVFDLENFAIQVRAETEEMYEYCRDIEQNIP
                     LDKAEFPMVDDQRICAYCNFRGICYPNLYPANL"
     gene            693..1019
                     /locus_tag="VI935_10540"
     CDS             693..1019
                     /locus_tag="VI935_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26071.1"
                     /translation="MNLRDLKWSAKEKKVAREAFDKAYKQEVRQIHKEVYEKVKSFRE
                     ENDVWKLHDYLNGKRKEIDEKYDYRYSVLIYVFARLMSESFIREEDLEGLSQDKLLNI
                     KGLLKI"
     gene            complement(1294..1875)
                     /locus_tag="VI935_10545"
     CDS             complement(1294..1875)
                     /locus_tag="VI935_10545"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLE26072.1"
                     /translation="MEREGHIHSTAVLKTFVKEMTLLPKSLILDLGCICDSNIELFTH
                     LGFKVFVKDFLNANVQALTNSSSIREINVDELQIDFEYPENFFDGILLWDVFDHFDFK
                     EAWLIVDNAKRILKEKGWALALFRPTNPTPFNTITRFRVVTSGDIKYETLPLITPRYK
                     IYYNRDVTDLFINFSSHTSYILKNRWREVIARK"
     gene            complement(2073..2606)
                     /locus_tag="VI935_10550"
     CDS             complement(2073..2606)
                     /locus_tag="VI935_10550"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HLE26073.1"
                     /translation="MWDKKESGVKYPKDKKELGVKYPEDNRESEIKYPEEIPVTPQVR
                     KLINPERGDVVKIGKSIFIKGEVSGGQDLIIDGRVEGEIQLKDNQVTIGENGKISGEI
                     HAKTIVIHGEVVGNMFAGEKLEIKASGALKGDITSPRLIIDDGAYFKGSIDMEVDGQK
                     RLERPATEILEVVKSED"
     gene            3056..4042
                     /locus_tag="VI935_10555"
     CDS             3056..4042
                     /locus_tag="VI935_10555"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HLE26074.1"
                     /translation="MNVYWISPARVERHQVEDLKSLLGREDGFVWVDLPECDEQASRV
                     LSEIFGFHPLAVEDSRKQTHVPKVHVYADHLFVILHAPEPESAGHIHLLELKQFVGRR
                     YLVTVHGPFDKDVPLDACLRETRTVLQRIEAGRYHPRLPAELSYAIVSALTRHTEALV
                     SALASKVATLERQVVEGKINSSEQVLEAMFNLRHELLTVRTVAAHSREIYSRLATLAP
                     RFIPIEERSFIEDIVDQFERIRSICNGEVEFLQGVIDFYQTRTITKLNFAIERLALIT
                     VLLLPVTAVASIYGMNIIVFEKSSIVQIGIVLVAMLIIVALIFRWAKRQGWW"
     gene            complement(4309..5283)
                     /locus_tag="VI935_10560"
     CDS             complement(4309..5283)
                     /locus_tag="VI935_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HLE26075.1"
                     /translation="MPNNLFIVDAHEDIAYHLSYFNRDFVNPSIPCMITLPWLKQGNV
                     RLVFNTIFVPAEFKPHKTLDIALHQLSIYDRIYDEHNDDIFQVKNPGDLFRLQKDSRI
                     GFLTLMEGADPIQEPKELNEFYKRGVRIIGLAWNDQNQYASGNNTDSGLTKQGIELIK
                     RMNDLRITLDLSHLNEKCFWEAIELCETIPIASHSNARSLTNHPRNLKDDQIRAISER
                     GGVIGLVLYNHFLKTCGENLTCGDENRTTLEDIFAHADYIIKLCGEDYVGIGSDLDGA
                     GVDDFPKEIRSVADLPKIGEFFLKKGYTEQRVKKIMNGNFLRVLKENS"
     gene            complement(5443..6669)
                     /gene="amrB"
                     /locus_tag="VI935_10565"
     CDS             complement(5443..6669)
                     /gene="amrB"
                     /locus_tag="VI935_10565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HLE26076.1"
                     /translation="MEYPKLRYVEAFPVEAQGQKLVCLRDPENLSDRVLFVSPEALFI
                     ISLFDGKHSILDIQEKYMRQFGKLIFRDEIQGLVNQLDEALMLDNKNYRNFKNKLEEE
                     FKNSRVRESSHAGLSYPQEANEIKNWLSSFFENAEKSHPSNSDLGKITGIISPHIDFR
                     RGGHSYALAYRELINRCDADVFIIFGTSHFANVENPFILTKKNFRTPFGEVETDAEIV
                     EKLVKQTECDLFEGEIAHRTEHSIEFQVVFLQYLYREIKNFKIVPILCNSFFRMIQNV
                     DSPMANTSVSMFLKGIKGIIEGLGDKAFAIAGADLAHVGLKFGDTEPVSQPALIWIKQ
                     RDLLSLSFTENLDAEGFFNTIKEEKDRRKICGLSPIYALLSTIKAERGKILDYDQALE
                     PDTGSVVSFASVGFYS"
     gene            complement(6837..>7117)
                     /locus_tag="VI935_10570"
     CDS             complement(6837..>7117)
                     /locus_tag="VI935_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HLE26077.1"
                     /translation="ASGGDGKYRGGDGIVRGYKFLSKSTVCVITERRRLAPYGLLGGK
                     PGKKGKNILIRNKKSKLIPPKATFEAKKADILRIETPGGGGWGKEVKS"
BASE COUNT         1923 a         1534 c         1424 g         2236 t
ORIGIN      
        1 gtacctgcat cccgaatacc aagaagtcca ggaaacattc aatgtcttcg acctggaaaa
       61 ctttgctatt caggttcggg ctgaaactga agagatgtat gaatactgca gggatatcga
      121 acagaacata ccgttggata aagcggagtt ccctatggta gatgaccaaa ggatttgcgc
      181 ttactgcaat tttaggggca tttgctatcc aaacctatat cctgccaacc tctgagtctc
      241 aaaaaataga aaataagggg cattgtctca actgtctgtt gcccaaatgt attttgtttt
      301 agtaacttgg gaaaagggga tccaacccat ttcttgttga agaatgcttt ccgcgttagc
      361 ttcgcctaac aagagaaacg gagaaacggg gtcaggcctt gaattgtgaa tgacattgat
      421 attgcttagt tttttacttc agccgaccac actccactgt aggtgtgcac agcaactcat
      481 gcactcgtgg ggctactttt cgttaacgct ttgcacgatt ctttcgtggc ggcttaactt
      541 gtcagttata ctgaaaaagc ttgcacataa atcagtttga catctgataa ccgacgtcat
      601 atattctaaa tttaatattg ttgttcaaat gtaagaaaaa gcaaagatat tcaaataaca
      661 gttaggtctt aaacacatat ggatgctatt atatgaattt acgtgattta aaatggtcag
      721 ctaaagagaa aaaagttgct agagaggctt tcgataaagc atacaaacag gaagtgcggc
      781 agattcataa agaagtgtat gaaaaagtaa aaagttttag agaagaaaac gatgtctgga
      841 aacttcatga ctatttaaat ggaaaacgaa aagagattga tgagaagtat gattataggt
      901 attccgttct tatttacgtc tttgcaagat taatgagtga aagctttata agagaggaag
      961 atttagaagg tctctctcaa gataaactgt taaacataaa aggcttattg aaaatataag
     1021 tgtatgcgga aaaatctacg gataagaatc cgtttgatat ttaacgacct gggtcatata
     1081 ttggaaggtc gttcagagtc taaggtctta aattattaat tacacctata tttcctaact
     1141 ctgtgcagcg tagagcgtta ccacaagaat atttagggat cattttttta aatcaacttt
     1201 gagattagtt gtctagttat tagttagttc tatttaacgc cattgctgca attgtagcaa
     1261 ccttttcgtg agttattaaa gttaacggct agctcacttt cttgctataa cctctctcca
     1321 tctgttcttg agtatatagg aggtgtggga agagaagttg ataaataggt ccgttacatc
     1381 tcgattgtaa tagattttat accttggggt tatgagtggc aaggtttcat actttatatc
     1441 accggaggtg actaccctga atctggttat ggtattgaac ggggttggat ttgtaggacg
     1501 aaaaagggct agagcccacc ccttttcttt taatatcctc ttggcattat ctactataag
     1561 ccaagcctct ttaaaatcaa agtggtcaaa tacgtcccag aggagaatac catcaaagaa
     1621 attttcagga tattcaaaat caatttgcaa ctcgtctaca ttgatttctc taatgctact
     1681 cgaatttgtc aaagcttgga cgttagcatt caggaagtcc ttaacaaaca ccttgaatcc
     1741 aagatgtgta aaaagttcaa tatttgagtc gcaaatacat cccaagtcta agataagtga
     1801 cttcggaagc agtgtcattt cctttacgaa agtttttaga acagcggttg aatgaatatg
     1861 cccttctctt tccattccct cttttcttga gatggatact tcgctcaaag tagttcgtcg
     1921 ttggtcgtta gtcatttgtt gttagacaaa aagaatcgaa agaaaaataa ccaatgacta
     1981 attcactcca ttacctgaat ctgctaacgg catccgttaa tccgtttatc acccgttgac
     2041 agattcctca ttatccgatt aatattaaaa tttcaatcct cgctcttaac tacctctaga
     2101 atctctgttg ccggtctctc gagcctcttc tgcccatcta cttccatatc tatgctccct
     2161 ttaaaataag ctccgtcatc aataatcagc ctaggtgagg tgatgtcccc ctttagagct
     2221 ccagaagcct taatctccag cttctctcct gcaaacatat ttccgactac ttcaccgtgg
     2281 atgactatag ttttggcatg tatttcaccc gatattttcc cgttttctcc aatcgtcacc
     2341 tgattgtcct taagttggat ctctccctct accctgccat caataattaa atcctgccct
     2401 cccgatactt cccctttaat gaaaatcgat tttccgattt tcactacgtc tcctctctca
     2461 ggattgatga gctttcgaac ctggggcgta acaggtatct cctcagggta tttaatctct
     2521 gattccctat tatcttcagg atatttaact cctagttcct tcttatcctt ggggtattta
     2581 acccctgatt ccttcttatc ccacatattt ttccctcctt tctttaagat ttaccagcct
     2641 accctctatt tattctctat ttccccttta cattttataa cataaatttc agcaaaatta
     2701 tagaaagact tattcctcag tttaaaaatt taatcgtgga ggtgttactt ctgcagcttt
     2761 tagtctgtat ttattgctat tgttgcgatt gtgacaatcc ttatttgtaa tttgagggga
     2821 gataaatagc acgagaaaaa gataactgca catcttttaa atatatcatg caacttagta
     2881 gaaccttaaa tttattccta gtctaccccg ggagttttca aagcggttga tttagaagga
     2941 acgtttgttg tatactgacc agcggcgaca gttgttacaa gaatttccgt tggagctata
     3001 tcactatcaa ctactactca tcaaacaaac ggagcgcttc aggagagggt gagggatgaa
     3061 cgtgtactgg atttcccccg cgagggtcga gcggcatcaa gttgaggacc tgaaatcctt
     3121 gctcgggcgc gaggatggtt tcgtatgggt agacctcccc gaatgcgacg aacaggccag
     3181 ccgagtgctg tcggagattt ttggttttca tccactcgcc gtagaggaca gtcgcaaaca
     3241 gactcacgtc ccgaaggttc acgtatatgc cgaccatcta ttcgtgatac ttcacgcccc
     3301 cgagccggaa agcgctggcc acatccatct cctcgaactc aaacagtttg ttggacggcg
     3361 ttatctggtt accgtccacg gtcccttcga taaggatgtc ccgcttgacg cctgtctccg
     3421 ggagacccgc actgtgctac agcgaatcga agcgggacgc taccaccctc gtttacctgc
     3481 tgagctctct tacgctatcg tctcggctct aacccgccac acagaagcgc ttgtatcagc
     3541 cctcgctagt aaggtagcca cactcgagcg gcaggtcgtg gagggaaaga ttaacagctc
     3601 cgagcaggtc cttgaggcaa tgtttaacct tcggcacgag cttctcacgg ttcggactgt
     3661 cgccgcgcat agccgagaga tatattcgag gttagccacg ctcgcgcccc ggttcatccc
     3721 gatcgaggaa cgctccttca ttgaggacat cgtagaccag ttcgagcgta tccgaagcat
     3781 ctgtaacgga gaggttgagt tcctccaagg cgtgattgac ttttatcaaa cccgcaccat
     3841 tacgaagctc aactttgcga tagagcggct tgctctcatt accgtgctcc ttctgccggt
     3901 aaccgcagta gcctcgattt atgggatgaa catcattgtg ttcgagaaga gcagcatcgt
     3961 ccagattggc attgtgcttg tagcgatgtt gattatcgtt gcattgattt tccgttgggc
     4021 gaagcgccag ggatggtggt gagggatggt ccaccaccca cgaggtcatg gggttggaat
     4081 tctggagaaa ctggaaacag agtaatataa gactcgcaca aagtcggggt aaatatcgcc
     4141 tgctaaaggc ttgcagtcta gaacagctta ggtgctcgta agtcagccgg tctttcttag
     4201 aatcactctg gttggcgaag ggatctttcc tgcacgcggt ttcggctgaa cacgaagcat
     4261 agtaagtttt ttgatatgtt atacaggtca ccacctcgat attagtaact atgaattctc
     4321 tttcagaacc ctgagaaagt taccgttcat aatttttttt acacgctgtt ctgtgtagcc
     4381 ctttttgaga aaaaattctc caatctttgg caaatcagct actgagcgaa tctcttttgg
     4441 aaaatcatcg actcctgccc catctaaatc gcttccgatt ccaacatagt cttctccaca
     4501 taattttatt atgtaatctg catgggcaaa tatatcttcg agtgtggttc gattttcatc
     4561 accacaggtg agattttcac cgcatgtttt tagaaagtgg ttatatagca caagcccaat
     4621 tacaccgcct ctttccgaga ttgcgcggat ttgatcatct ttcaaatttc ttggatggtt
     4681 tgtaagactt ctggcgtttg aatgagaggc gattggaata gtctcacaga gctctatggc
     4741 ttcccaaaag catttttcgt taagatgaga taaatcaaga gtaattctta aatcattcat
     4801 tcgtttgatt aattctatgc cctgttttgt aagacctgaa tccgtgttat taccggatgc
     4861 gtattgattt tggtcattcc aagcaagccc tataatccga acacctcttt tgtaaaactc
     4921 gtttaattct ttaggctcct gtataggatc ggctccttcc attagtgtaa gaaacccgat
     4981 tcttgaatct ttttgtagcc tgaataggtc accagggttt ttaacctgaa aaatatcgtc
     5041 gttgtgctcg tcataaatcc tgtcgtagat tgataattgg tgaagggcga tatctagtgt
     5101 cttatgaggt ttaaattctg cgggtacgaa gattgtgtta aaaactaatc tgacgtttcc
     5161 ctgttttaac caggggagtg taatcatgca tggaatggag ggatttacga aatccctatt
     5221 aaagtagctt aggtgatatg ctatatcttc gtgcgcgtct acgatgaaga ggttattcgg
     5281 cattgtgaat ctttagctac gaataaacac gaatttacac taataaaatg gtgcatgata
     5341 catgaagttt tactgcatca tgcatcttgt atcgtgtatc tcgcgtctgc ttattttcgt
     5401 tcatgtccat ttgtgccaat tcgtggctga ataatttttt ggctacgagt aaaaccccac
     5461 gcttgcaaag ctaacgactg agcctgtatc aggctcaagg gcttgatcgt aatccaatat
     5521 ttttcctctt tcagctttta ttgtagaaag aagcgcataa atcggcgaaa gaccgcatat
     5581 ctttctccgg tctttctcct cttttattgt gttaaagaac ccctcggcat ctaggttctc
     5641 agtaaatgaa aggctcagta ggtctctttg tttgatccat ataagcgcgg gctgtgatac
     5701 aggctcagta tcaccaaatt tcagtccgac atgggctagg tcagctccgg ctatggcaaa
     5761 tgctttatct cctaagccct ctatgattcc tttaatcccc tttagaaaca tcgaaacact
     5821 tgtgttggcc attggagaat ccacattttg tatcattcta aagaatgagt tgcaaagaat
     5881 gggcactatc ttgaagttct taatctctct ataaagatac tggaggaata ctacctgaaa
     5941 ttcgattgag tgctcggtgc gatgagcgat ttctccctca aagaggtcac attcggtctg
     6001 cttgacaagc ttttccacga tctcggcatc ggtttcgact tcgccaaaag gagtcctgaa
     6061 atttttcttc gtaagaatga acgggttttc tacattagca aagtgggagg ttccaaaaat
     6121 tataaaaaca tctgcatcac atctgttaat taattctctg taggcaagag cataagagtg
     6181 tcctcccctt ctaaaatcta tgtgaggaga aattattccg gtgatcttcc caagatcaga
     6241 attagatgga tgagatttct cggcattttc aaagaatgag ctaagccaat ttttaatctc
     6301 atttgcctct tgagggtacg acagtcccgc atgacttgat tccctcaccc tggaattctt
     6361 aaattcttct tcgagcttat ttttgaagtt tctataattt ttattgtcga gcatcagagc
     6421 ttcatccaat tggtttacta atccctgaat ctcgtctcgg aaaatgagct ttccaaactg
     6481 cctcatatac ttttcctgta tgtcaaggat tgagtgtttt ccatcaaaaa ggcttattat
     6541 gaatagtgct tcgggcgaga cgaaaagtac cctatcactt aaattttcgg gatctcttaa
     6601 gcaaacaagc ttctgcccct gagcctctac gggaaaggcc tcaacatatc ttagctttgg
     6661 gtactccatg gcgttaaatt ttacctaagg ctcagcaata aagacactga attagaattc
     6721 agaagtcaga attcagaagt tttttggctt taggattcta tcttcaggat tcaggtttct
     6781 gaatctactt taaaacttta agtccatggt tttcctttgt gtcttcgtgg cttatcctaa
     6841 gattttactt ctttccccca tccgcctccg cccggagttt ctattcggag aatatccgct
     6901 ttcttggctt caaaggttgc cttaggtgga attaatttag actttttatt tctaattaaa
     6961 atattctttc ctttcttccc cggtttccca ccgagaagtc cataaggagc aagtcttctc
     7021 cgctctgtaa ttacacaaac tgttgattta gaaagaaact tataacctct tacaattcca
     7081 tctccgcctc tatacttacc atctccaccg cttgctc
//
LOCUS       DATDRL010000317         5033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3521871, whole genome shotgun sequence.
ACCESSION   DATDRL010000317 DATDRL010000000
VERSION     DATDRL010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3521871"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /gene="glmS"
                     /locus_tag="VI935_10575"
     CDS             complement(<1..1002)
                     /gene="glmS"
                     /locus_tag="VI935_10575"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HLE26078.1"
                     /translation="MCGIVGYIGSAGKTVNVLVEGLRRLEYRGYDSSGIAILENGHAK
                     VLKSEGKLDNLLKKLSLTSFNGSLGIGHTRWATHGIASERNAHPHVSGGTAVVHNGIV
                     ENYQELKDELKMKGYVFNSDTDTEVISHLIEDFSANGSSFEESVRGTFKLIEGSYAVA
                     IVSEREPDRIIGVRKFSPLILALGENENFIASDIPAVLPYSRKIIFLEDGDVAILERD
                     RFRITDLDGHEVKREIFSINWDPVVIEKGGYKHFMLKEIHEQPHAVLDTIRGRVSEED
                     GNVYFEDLEDVAKGVRRIVAIGCGTSYHSCLVGRHMIENIAKVPVELDFASEIRYRDP
                     "
     gene            complement(1003..2388)
                     /gene="glmU"
                     /locus_tag="VI935_10580"
     CDS             complement(1003..2388)
                     /gene="glmU"
                     /locus_tag="VI935_10580"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HLE26079.1"
                     /translation="MGFSVIVLAAGKGKRMKSELPKVLHPILGRPMLGYVIDAVMQIS
                     PERIIVVVGYGADKIKETLSSIKIKYALQGKQLGTGHAVRCAKEAFQDYNGKILILNG
                     DFPLIRSETLKGFVENHKKSNAEISLLTAFLDSPEGYGRIVRNGKGDVIKIVEEKDAT
                     PKERKIKEINSGAYCVGSSFLWDVIGKIGSKNEQKEYYLPDIVNIATKSGKKVSGFIV
                     PDSEEALGVNNREELARVEGRLRKRTNEKLMFSGVTILNPESTYISPSVSIEADTVIY
                     PNTFICGNTKVGRDCRIGPSVWIEDSEIGDEVTIAFSSCIIKALIEGKAKIGPFAHLR
                     PESQILSGAKVGNFVEIKKSKIGRGSKVPHLSYVGDASIGEGVNIGAGTITCNYDGFH
                     KHETIIEDNVFIGSDTMLVAPIKVGRGATTGAGSTITKDVPPGALAIERSKQQVITGW
                     KRKPKGKKGNE"
     gene            complement(2681..3112)
                     /locus_tag="VI935_10585"
     CDS             complement(2681..3112)
                     /locus_tag="VI935_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HLE26080.1"
                     /translation="MRGVNKVILIGNLGRDPEIRYTTSGQAVANFTLATTEVRQNKSG
                     EKDEFTEWHRIVAWGRLAEICGEYLSKGKTVYIEGALRTRSWEDKEGRKRWTTEVVAQ
                     NMQMLGPFQERAASGTVAEGKLTEDFESGEESFGSDEDVPF"
     gene            complement(3207..3818)
                     /locus_tag="VI935_10590"
     CDS             complement(3207..3818)
                     /locus_tag="VI935_10590"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HLE26081.1"
                     /translation="MYSNEMEELERIINGRKGVRIKRGESFISAAVMVILREERNGYS
                     MLFIKRPESQGDPFSGHMAFPGGKGKVEDKSKLETAIRETIEETGIDLNRIGRILGEL
                     DDVSPINPKANHYVVTPYIAYLTEDSEIKPNEEVAFAIWIPVFHFRNEKSFERRVVEK
                     HGMELEEFVYHYQNHVIWGMTARILHQFLSLAGHLFWIRGRFN"
     gene            complement(3915..4430)
                     /locus_tag="VI935_10595"
     CDS             complement(3915..4430)
                     /locus_tag="VI935_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HLE26082.1"
                     /translation="MKDKIIIMQGDITDIEVDAIVNAANTRLLLGSGVAGAIRRKGGD
                     SIQRECDKIGSIPLGQAAVTGAGKLNAKYVIHAAGMHIGGNVTEEALTGSTKNSLLRA
                     DEKGVKTIAFPAVGTGVGGFSINRCAQVMIDIVVEYLKNEKTVIEKVYFVLFDMEAYK
                     AFNDYLRKATL"
     gene            4642..>5033
                     /locus_tag="VI935_10600"
     CDS             4642..>5033
                     /locus_tag="VI935_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26083.1"
                     /translation="MRISFYRHSLLSRGGDRMVIEYANYLAEKGHEVVLWYNNLKTVY
                     KLHPKLKLIKIPILKKLGTIIYATINRFNSDVVIVDIIALASLLSLRNRSRLIFFAQG
                     YDEFYYKSPFKKLLIKMVYLFCLKLMKV"
BASE COUNT         1428 a         1225 c          799 g         1581 t
ORIGIN      
        1 ggggtctcta tatcgaattt ccgatgcaaa atccaattct accggaacct tagctatatt
       61 ttctatcatg tgcctgccaa caagacatga atgataggag gttccgcacc caatcgctac
      121 tattcttctt acaccttttg caacgtcttc caaatcctca aagtacacat taccatcctc
      181 ttccgatacc ctgccgcgaa tggtatcgag cacagcgtgt ggctgctcat ggatttcctt
      241 tagcatgaaa tgcttatagc cgcctttttc tatgacgaca gggtcccagt tgattgagaa
      301 tatttctctc ttaacttcat gtccatcgag gtctgttatt ctgaatctat ccctctcaag
      361 tatagcaacg tcgccgtcct ctaaaaagat aatcttcctg ctatatggaa gaacggctgg
      421 aatatcagac gctatgaagt tttcattttc accaagagca agaatgaggg gtgagaactt
      481 tcttactcct atgatcctgt ctggctccct ttcagaaaca attgctaccg cataggagcc
      541 ttcgataagt ttaaaagtgc ctcttacaga ctcctcaaat gatgacccgt ttgctgaaaa
      601 gtcttctata agatgtgaaa ttacctctgt atctgtgtca gaattaaaga cgtatccctt
      661 catcttgagt tcgtccttaa gctcttgata attctctaca atcccattat gaaccaccgc
      721 tgttccaccc gagacatggg gatgagcatt tctctccgac gcaattccgt gggttgccca
      781 tcttgtgtgt ccaattccta aggaaccatt aaaagaagtt aaactaagct ttttaaggag
      841 gttatcgagt tttccttcgc tctttagaac cttagcatga ccattttcaa ggatggcaat
      901 gccagaagaa tcataccctc tgtattcaag ccttctgagt ccctcaacca atacatttac
      961 tgtttttcct gcgctgccta tgtatcctac aataccacac atctattcgt ttcccttttt
     1021 tcctttaggt tttcttttcc acccagttat tacctgttgt tttgatctct caattgctag
     1081 agctcctggc ggaacatcct ttgttatagt tgaacctgcc ccggttgtag ccccccttcc
     1141 aacctttata ggggcaacaa gcattgtgtc actacctata aaaacattat cctctatgat
     1201 tgtctcatgc ttatgaaacc catcataatt acatgtgatc gttccagccc cgatgttcac
     1261 tccctctcca atgcttgcat caccaacgta ggatagatga ggaactttcg aaccacgtcc
     1321 tatttttgac ttcttgatct cgacaaagtt tccaacttta gcccctgaaa gtatttgtga
     1381 ttccggacga aggtgggcaa agggccctat tttagcttta ccttcaataa gtgcctttat
     1441 tatacaagag gaaaacgcta tagtgacctc atcaccgatc tctgaatcct caatccacac
     1501 agatgggccg atcctacaat cccttcctac cttagtattt ccgcaaataa aagtgtttgg
     1561 ataaattaca gtatcagcct cgatggaaac acttggagaa atatatgtac tctctggatt
     1621 cagtatcgtt accccagaga acataagttt ctcatttgtt cttttcctca aacgcccttc
     1681 cacccttgca agctcctctc gattattcac tccgagcgcc tcttcgctat cagggacaat
     1741 aaatccactg acctttttcc cgctcttagt tgcaatatta acaatatctg ggagataata
     1801 ttccttctgc tcatttttac taccaatctt cccaattaca tcccatagaa aactcgatcc
     1861 gacacaatat gcgcccgagt ttatctcttt gatcttcctc tccttgggag ttgcatcctt
     1921 ctcctctaca atttttataa catccccctt tccatttctt acaatccttc cataaccctc
     1981 agggctatct agaaaagcgg taagaagaga aatctctgca ttacttttct tgtgattctc
     2041 tacgaaacct tttaaggttt cagaacgaat gagaggaaaa tccccgttta atataagaat
     2101 cttcccatta taatcctgaa atgcctcctt agcacacctg acagcatgtc ctgtaccaag
     2161 ctgcttaccc tgcagggcgt atttaatctt aatggatgaa agcgtctctt taattttatc
     2221 cgcaccatac cctactacta caattattct ctccggtgat atttgcataa ctgcatcaat
     2281 aacgtaacca agcataggac gccccaatat tggatgaagg accttaggaa gctcagattt
     2341 cattctcttt cccttgccag cggcaaggac tataaccgaa aagcccatta tgttatttac
     2401 ataccatccc ttatcgtctt aaagaatgac tggatagtta gcataaagag tacttatcgt
     2461 acattttaaa aatataaaag gaatcataag ataactctgg gttttactta tgtactttat
     2521 atttaaacaa atactaggtt atttatgcac ctcaacactc aaaaaatcca tttctccggt
     2581 tgaataaatt cagctttctt agcatgaaac aataagttat accaattttt tttacctaga
     2641 ctcatacata aactactgtc tcaagaaaaa tgagttacct tcagaatggg acgtcctcgt
     2701 cagaaccaaa ggattcctca ccactttcaa agtcctcagt aagtttcccc tcagccacag
     2761 tacctgacgc agctctttct tgaaatggac ctagcatctg catattctgt gcaactacct
     2821 cggttgtcca tctctttctt ccctctttat cttcccagga gcgagtccta agagctccct
     2881 ctatataaac agtttttcct ttagaaaggt attcgccaca tatctctgct agtcttcccc
     2941 atgcgacaat acgatgccat tctgtgaatt catccttctc accgctttta ttctgacgca
     3001 cctcagtagt agctagcgta aagttagcaa ccgcttgtcc actcgtcgtg taccttattt
     3061 cagggtcacg tcccaagttg ccgataagga ttaccttatt tactcctctc attttatacc
     3121 ccctgtcctg ctctactttc aaagaatttt atcatcgggt aataagaaaa tccatagatc
     3181 aaggttttgg taccgacgaa tagctgttaa ttaaacctac ccctgatcca aaaaagatgg
     3241 ccagctaagg agagaaactg atgaagaatt cttgcagtca ttccccaaat cacgtggttt
     3301 tgataatggt agacgaattc ttctaactcc atcccgtgtt tttcaacgac tctcctttcg
     3361 aacgattttt cattcctgaa atgaaatact gggatccaaa tagcaaaagc cacctcctca
     3421 ttcggcttaa tttccgagtc ttctgttaga taggcgatgt acggggtaac aacatagtga
     3481 tttgcctttg gattaatcgg gctaacatca tccaactccc caaggattct tccaatccta
     3541 ttcaggtcaa tgcctgtttc ctcgatagtt tcacgaatcg ccgtttcaag cttgctttta
     3601 tcctctactt tccccttacc cccaggaaaa gccatatgcc cagaaaaagg gtcaccttga
     3661 ctttcagggc gtttaataaa gagcattgaa tatccattac gctcttctct aaggattacc
     3721 attactgctg cggatataaa actctcaccc cttttaatcc taacgccttt tcttccattt
     3781 ataattctct caagttcctc catttcgttt gagtacataa attccgacct aatactgtaa
     3841 tctcttgata gactaggaga agtataacaa acaggaaaac ttattaaaga ttaagaccaa
     3901 atccttattt acacttaaag ggtggctttc ctgagataat cattaaaagc cttataagcc
     3961 tccatgtcaa aaagaacaaa atataccttc tcgatgacag ttttttcatt ctttaaatat
     4021 tcaacaacta tatcaatcat cacttgagca catctattta tagaaaaacc cccaactccg
     4081 gttccaaccg cagggaaggc aattgttttt accccctttt catcagctct taaaaggcta
     4141 tttttagtcg atcctgtcaa tgcctcttcg gttacattac cacctatgtg catcccggca
     4201 gcgtgaatca cgtattttgc attaagtttc cccgctcctg taacggccgc ttgacctaaa
     4261 ggaatagaac cgatcttatc gcattccctt tgaatagaat ccccgccctt tcttcttatg
     4321 gcaccagcaa ccccactgcc aagaagaagg cgtgtattcg cagcgtttac aattgcatcc
     4381 acctcaatat cagtaatgtc gccttgcatt atgattattt tgtctttcaa atccattgag
     4441 acaccttttg ctaatatttt acaaatctaa aaaagaggta gcaagcatag ttgggaatta
     4501 tgaattaggc attggaaatt ggggctaatt cctaatccca aattcccaat tccaattaat
     4561 tgcactgctc aaagaaaacg gctagaattt aacaagacga tttttaatga tataaaagtc
     4621 aacaccggag attagaagta ggtgagaatc tctttttata gacattccct tctaagtaga
     4681 ggcggggata gaatggtaat cgaatacgct aattatcttg ctgaaaaggg acatgaagtg
     4741 gtgttgtggt acaataactt aaagacagtt tataagctcc atccaaaact caagttaata
     4801 aaaattccaa ttctgaaaaa actcggaaca attatttatg cgactataaa tagattcaac
     4861 tcagatgttg tcatagttga cataatcgcc ctagcttcgc tactgtcttt aagaaaccgc
     4921 tccaggctta ttttctttgc tcaaggttat gatgaattct actataaaag cccttttaaa
     4981 aagctactta ttaaaatggt atacctcttt tgtttaaaac ttatgaaagt aaa
//
LOCUS       DATDRL010000318         8796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3279770, whole genome shotgun sequence.
ACCESSION   DATDRL010000318 DATDRL010000000
VERSION     DATDRL010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8796
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3279770"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VI935_10605"
     CDS             <1..183
                     /locus_tag="VI935_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HLE26084.1"
                     /translation="FCRHYKHQWGSNTVRYASCGIKQPRQMRRPMLSNRFTTNWDEIL
                     KVKPSFPIKFDSEAST"
     gene            239..385
                     /locus_tag="VI935_10610"
     CDS             239..385
                     /locus_tag="VI935_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26085.1"
                     /translation="MEEFKAIYDEKEDCLYLTKTNGSVHVEDTEIGKGIYPKKCTYPI
                     STVT"
     gene            complement(496..675)
                     /locus_tag="VI935_10615"
     CDS             complement(496..675)
                     /locus_tag="VI935_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HLE26086.1"
                     /translation="MTQEIRCRDVGVDCDWVASGETEEEIMKKVAEHAKKAHGMDEIP
                     QELVQRVRAAIHTQK"
     gene            1063..1299
                     /locus_tag="VI935_10620"
     CDS             1063..1299
                     /locus_tag="VI935_10620"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HLE26087.1"
                     /translation="MRSGVANELMEPIQDRMPVIPHEKDEGMWLDPQLSDTDKLSTLL
                     KPYPSKKMEAYKVSTFVNSPKNESPNCIEPIEDY"
     gene            complement(1743..1817)
                     /locus_tag="VI935_10625"
     tRNA            complement(1743..1817)
                     /locus_tag="VI935_10625"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1780..1782),aa:Asp,seq:gtc)
     gene            complement(1891..1965)
                     /locus_tag="VI935_10630"
     tRNA            complement(1891..1965)
                     /locus_tag="VI935_10630"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1931..1933),aa:Val,seq:tac)
     gene            complement(2094..4526)
                     /gene="lon"
                     /locus_tag="VI935_10635"
     CDS             complement(2094..4526)
                     /gene="lon"
                     /locus_tag="VI935_10635"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HLE26088.1"
                     /translation="MFFKLDGERKQELAMIPLLPLRDVVIFPHMVAPLFVGREKSIRA
                     LEEAMRKDKEILLAAQRDAKTNDPNEEDIYKIGTIGTIIQMLRLPDGTVKVLVEGKRR
                     AQILRYVPNPSFFIVEAERISEVDDAGVETEALMRTLVTAFENYVKLNKKIPSEVLVT
                     ISSIDEPSRLGDTIASHLGLKLQDKQEILECASPAERLEKLYEKLQGEVEILQIEKRI
                     RTRVKKQMEKAQKEYYLTEQMRAIQKELGEKDDLKSEIQEFEERLKQKSLPEEVEQKV
                     KRELKKLKMMSPMSAEATVVRNYIDWLLSIPWTNEKTEDRLDLDEATKILEEDHYGLE
                     KPKERIVEYLAVRALTKKLKGPILCFVGPPGVGKTSLGKSIARAMGRKFVRVSLGGVR
                     DEAEIRGHRRTYIGALPGKIIQGMRKAGTINPVFLLDEIDKLGMDFRGDPAAALLEAL
                     DPEQNHMFNDHYIDADYDLSSVLFITTANILHTIPWALQDRMEIIRLPGYTEDEKLEI
                     AKRFLVPKQLDAHGLKPENFKINDNGILSVIRQYTRESGVRTLERELATICRKVAKEV
                     VKHGPDHAMKINSGAIHKFLGIPKFRFGEIEEKDQVGIATGLAWTEVGGELLTIEVAI
                     VPGKGTFTITGKLGEVMQESAQAAMSYVRSRAEKLGLERYFYQKVDIHIHVPEGATPK
                     DGPSAGIAMATAIVSALVKKPVQHDLAMTGEITLRGRVLPIGGLKEKILAAHRGRVKR
                     IITPLENEKDLRDISDKILKDFELKLVEHMDEVIREAVVSEEPVLADIVTPPLGSLPI
                     DEGIVQTNINLS"
     gene            complement(4556..5833)
                     /gene="clpX"
                     /locus_tag="VI935_10640"
     CDS             complement(4556..5833)
                     /gene="clpX"
                     /locus_tag="VI935_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HLE26089.1"
                     /translation="MTTRREGRDGNLYCSLCGKSQKEVRKLIAGPTVYICNECIELCN
                     EIIAEEEAKEGHLRRRYSTLPTPYQIKAFLDEYVISQERAKKILAVAVYNHYKRIELN
                     AYSGKRSSDVDVQKSNILLIGPTGCGKTLLAQTLAKFLDVPFTIVDATCYTEAGYVGE
                     DVENMIVSLLQAADYDLERASKGIIYIDEIDKLARKSGDSPSITRDVSGEGVQQALLK
                     IIEGTKANVPPKGGRKHPQQEFIQVDTTNILFVCGGAFCGLEDIIGRRIGEKSIGFEA
                     TLGRREERGLGEILKEIQPEDLIKYGMIPEFVGRVPVVATLDELDEKTFVEILTKPKN
                     AVVKQFQKLMELEGVELEFTEGALKTIAHEAIRRKTGARGLRSIIETIMLDISYEVPS
                     LKGLRRCLITEELVGGTGVPLFFYEEERRLGSG"
     gene            complement(5830..6429)
                     /gene="clpP"
                     /locus_tag="VI935_10645"
     CDS             complement(5830..6429)
                     /gene="clpP"
                     /locus_tag="VI935_10645"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HLE26090.1"
                     /translation="MSSYVPIVIEQTNRGERAYDIFSRLLKDRIVFVGSPITDGVADT
                     VIAQLLFLESESPEKDIYVYVNSPGGHVTAGLAIYDTMQYIKPDVATICIGQAASMAA
                     VLLAAGIKGKRYALPHARILLHQVLGGVEGQATDIEIHAKEILRVREDINRILAKHIG
                     QPLERIARDTERDFFLTAKDALEYGIVDAIITKREEATK"
     gene            complement(6453..7745)
                     /gene="tig"
                     /locus_tag="VI935_10650"
     CDS             complement(6453..7745)
                     /gene="tig"
                     /locus_tag="VI935_10650"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HLE26091.1"
                     /translation="MKVNVESLSNTERKVEVFIPKEEVIGKLEEVFKELEREAKIKGF
                     RPGKAPRRVIEATYGGYIFEEVSSRLVSQSFEKALEEVSIIPISRPRITKDKIERDKE
                     FHYTAVFEIIPEFEVKDYIEIELKKQIREVKEEDIERVLNQIRERGAQAKPIGEDREV
                     RTGDYLIIDYVGTVDGKPIKDLKAKDAQVIVGDKKLIAEFEDNLIGIRKGEEKEFEIT
                     YAEDFQMKDVAGKTVKFTVTVKEIIEKILPELDDDFSKDLGEENLEGLKKKIREDLEK
                     RFNKESEERLKGELIKVLLGRNPLDVPPSLIENETLRLKREFSLNLERQGLKLPALNE
                     EVEGNFRNRAEQNVKASIILGAIARKEGVQVDEDEVDNKLTEISKTAAVPFQKVREIY
                     EKNNLIDSLKASLVEDKVLNFLIEKSDVKEETQIDKEG"
     gene            complement(7933..8015)
                     /locus_tag="VI935_10655"
     tRNA            complement(7933..8015)
                     /locus_tag="VI935_10655"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7979..7981),aa:Leu,seq:tag)
     gene            complement(8066..8139)
                     /locus_tag="VI935_10660"
     tRNA            complement(8066..8139)
                     /locus_tag="VI935_10660"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8103..8105),aa:Lys,seq:ctt)
BASE COUNT         2363 a         2146 c         1488 g         2799 t
ORIGIN      
        1 ttctgtagac actataaaca ccaatgggga agcaacacag ttcgatatgc ctcatgtggt
       61 attaagcagc cccggcagat gcgtaggcct atgctctcaa ataggtttac aaccaactgg
      121 gatgaaatac taaaggtcaa accctcattc cctattaaat ttgatagtga agctagtact
      181 taaaggccat agctaatgaa gctttggcgt tatcaaatcc ttaacttagg agatagtaat
      241 ggaagaattt aaagccatat atgatgagaa agaagactgc ttatatctta caaagacaaa
      301 tgggagtgtc catgtcgaag atacagaaat tggtaaaggt atttatccta aaaaatgcac
      361 ctatcctatt tctactgtta cttgaaataa gatagatgca tctaaagata ggtctatagt
      421 atttgaaact ttttcctatt tcttaggaaa gtttaatttc aaacaccttg aataatcagg
      481 caacggcttc tttcattact tctgggtgtg aatggctgca cgtactctct ggacaagctc
      541 ttgtggaatc tcgtccattc catgggcttt tttagcatgt tcagcaacct tcttcataat
      601 ctcttcctct gtttcaccac ttgctaccca gtcacagtcc acacctacat ccctacatcg
      661 aatttcttgt gtcattttaa acctccacaa tttgtattta gaagacgtcc attgtgctac
      721 aaatggcatc tcgtgtcaaa tgtagcatac aaattaacaa gaaacaaaaa ttcattcatc
      781 accatccctg tttttatgta tccctaaaat gctaactatg tccatttttt tctagattcc
      841 attcaacaaa agtagaagct gcaaagacat ttggatttta ctttttcttc atctcatttt
      901 atgcacgaac cttttctatt gctattctct cacaaaaagg aatcccattt caaacatacg
      961 tataatagtt cattgccaat gaaacaagtg agaggggtaa ttagtaagga tgatgcaaat
     1021 atctttaaga aattgcaacg gtctcttgta gcctaatgcc tgatgaggtc tggagttgca
     1081 aatgagttga tggaacccat tcaagatagg atgcctgtta tcccgcatga gaaagacgaa
     1141 ggtatgtggc tcgaccccca attaagtgat acggataagc tatcaacatt attgaagcca
     1201 tacccatcaa aaaaaatgga agcttataag gtgtctactt ttgtaaactc acctaagaat
     1261 gaaagtccaa attgtattga gccgattgaa gattactaag aacgcatgcg ttccaagatt
     1321 tatgaaaaac caagaaatgg gctttttcgt gattcactgc taacagattt tgcgggctaa
     1381 tttccctaaa aattgaatta agtaatcgaa actgtttcat ttctagtcaa tttcattgct
     1441 aacgtttttg ctaacagatt tttccaattt tggggtatct agagtttcta cagcactttt
     1501 taaagccttg ggcgtagggt gagaatatct catagtagtt tgtatttttg aatgccctaa
     1561 gagctttgaa actgttacaa tgtcaactcc agaaagcaca agacgagttg caaaagtgtg
     1621 tctaagtgta tgaaaagaca gattttttat gccagctttt ttacaggcat tttcaaagga
     1681 tgttctgaaa cctttaacag ggatgccctt ataagtgaag acaaagaaat agagatcgga
     1741 aacggggccg acgggactcg aacccgcgac ctctggcttg acaggccagc gttctaaccg
     1801 agctgaacta cggcccctga aattagttgc taatcgatag ttaaggactt ctgactatta
     1861 gactatcgac tagcaactat tgactaaaca tggtgggcgg taccggtttc gaaccagtga
     1921 cctcttgctt gtaaggcaag cgctctccca ctgagctaac cgcccttgct cttaagccct
     1981 ttctacacaa ctatttagtc actaattgaa acgaatttaa acgaataaaa cgatgcaaga
     2041 tgcgtgaatc ctctattgtg tatcctgcat cctgtatcaa ttttcagggc ttgtcaagaa
     2101 agattaatat tggtctgaac tataccctca tcaataggaa gagagccaag gggtggagtc
     2161 acaatatcag ccagtacggg ttcctcagac acgacagcct ccctgatcac ctcatccatg
     2221 tgctcaacca atttaagctc aaaatccttc aaaatcttat ctgatatatc cctaagatcc
     2281 ttttcgttct caagaggtgt aataatccgc ttcacccttc cgcgatgagc cgcaaggatc
     2341 ttttccttga gtccgccaat aggaagcacc cttccacgga gggtaatttc ccctgtcata
     2401 gccaaatcat gctgcaccgg tttctttaca agagccgata cgatagctgt tgccattgcg
     2461 ataccagccg aaggcccgtc ttttggagta gccccctccg gtacgtgtat gtgtatgtct
     2521 accttctgat aaaagtaacg ttccagccca agcttttccg ctctagacct cacataactc
     2581 attgcagcct gtgcggattc ctgcattacc tctccgagtt ttcctgtaat tgtaaaagtc
     2641 cctttgccag gaactatagc aacctcaatc gtaagtagct cgccaccaac ctcggtccat
     2701 gcaagacctg ttgcaatacc aacttggtcc ttctcctcga tctctccaaa cctgaacttt
     2761 ggaattccaa gaaacttgtg gatagcacct gaattaatct tcatcgcgtg gtcagggcca
     2821 tgcttaacaa cctctttagc tacctttcta caaattgttg caagctcacg ttcaagggtt
     2881 ctcacgcccg attcacgtgt gtactgcctt atcacactta atatcccgtt atcgttaatt
     2941 ttaaagttct ccggttttag tccatgagcg tcaagctgct tgggaaccag gaaccttttt
     3001 gcaatctcaa gcttttcatc ctcggtgtag cctgggagtc gtatgatctc cattctgtcc
     3061 tgaagcgccc agggaatagt atggagtata tttgcagtgg ttataaaaag cacacttgaa
     3121 agatcataat ccgcgtcaat gtagtgatca ttaaacatat gattctgctc agggtctaaa
     3181 gcctcaagga gggccgccgc tggatcaccc ctaaaatcca ttccgagttt atcaatttca
     3241 tcgagcaaga aaactggatt aatagtacct gccttcctca tcccctgaat aatctttcca
     3301 ggaagggcac caatatatgt gcggcgatga ccccggattt cagcctcgtc tcttacaccg
     3361 ccaagggata cccttacaaa ctttctcccc attgcacgcg caattgattt accaagcgat
     3421 gttttaccaa cccctggagg cccaacaaag cataggatcg gtcccttaag tttctttgta
     3481 agagcgcgaa cggcgaggta ttcaactatt ctctcttttg gcttttcaag cccataatgg
     3541 tcctcttcta gtatcttagt cgcttcatca agatcgagtc tatcttcagt cttttcattt
     3601 gtccaaggaa ttgagagaag ccagtcaatg tagtttctaa ctacagtagc ttcagccgac
     3661 ataggagaca tcatcttaag ctttttaagc tcacgcttaa ccttttgttc aacctcttcg
     3721 ggaaggctct tctgttttaa cctttcttca aactcctgaa tctctgactt aaggtcatcc
     3781 ttctcaccca actccttttg tatagccctc atttgttcgg tgagatagta ttccttctga
     3841 gccttctcca tctgcttctt tacccttgtt ctaatcctct tctcaatctg aagtatctca
     3901 acctcaccct gtaatttctc atatagcttt tcaagcctct ctgcagggct tgcacactcg
     3961 agtatctctt gcttatcttg aagtttaagc ccaaggtgcg aagcaattgt atcccctagg
     4021 cggcttggtt catcaatcga ggaaatagtg accaaaacct cagatgggat ctttttatta
     4081 agctttacat aattttcaaa cgcagtaaca agggtacgca ttagggcttc ggtttcgacc
     4141 cctgcgtcat ccacttctga aatcctctcg gcctcgacta taaaaaagct tggattagga
     4201 acatagcgaa gaatttgcgc ccttcttttt ccctctacca gtaccttaac agttccgtca
     4261 ggaaggcgga gcatctggat tattgttcca atcgttccaa ttttatagat atcctcttca
     4321 tttggatcat tggtcttggc atccctttga gcggcaagaa ggatctcttt atcctttctc
     4381 atcgcctctt caagcgctcg aattgacttc tcgcgtccta caaaaagtgg ggctaccata
     4441 tgcggaaaga tcaccacatc ccgaagcgga agaagaggaa tcattgctaa ttcttgtttt
     4501 ctttctccat caagcttaaa aaacattggc atcttattct ccttaactac tcaatttatc
     4561 cagaacctaa acgcctttcc tcctcataga agaaaagtgg aacacctgta ccccctacca
     4621 actcctccgt tattaaacaa cgcctaagcc cttttagtga tggaacctcg taagagatat
     4681 caagcatgat agtttcaatt atagacctta gcccacgagc cccagtcttt cttctaattg
     4741 cctcatgagc gatagtcttt aatgcaccct ctgtgaactc tagctcaaca ccttcgagct
     4801 ccataagctt ttggaactgt ttcacaactg cattctttgg ttttgttagt atctcaacaa
     4861 aggtcttttc atcaagctca tccaaggtcg caacaacagg gacccttcct acaaactccg
     4921 gtatcatacc atactttatc aaatcctcag gctgaatctc tttcaaaatc tcaccaagac
     4981 ccctttcctc tcttcttcca agtgtcgcct caaacccgat tgacttttca ccaatccttc
     5041 gccctattat atcctcaagc ccacaaaagg ccccaccgca gacaaacagg atgtttgtcg
     5101 tatcgacttg aatgaactcc tgttgtggat gctttcttcc tcccttaggt ggaacatttg
     5161 cctttgtacc ctctataatc tttagaagag cctgttgaac cccctctccc gaaacatccc
     5221 tcgtaatcga aggactatcc ccagattttc gggcgagctt atctatctca tctatatata
     5281 taatcccttt tgaagccctt tcaagatcat aatctgcagc ctgcaacagg ctcacaatca
     5341 tattttcaac atcctcacct acataacccg cctctgtgta gcatgtcgca tcaactatag
     5401 taaatggaac atcaaggaat ttcgcaagcg tctgtgccaa aagggttttt ccacaaccag
     5461 ttggacctat aagaaggata ttgctctttt gaacatcaac atcgctactt cttttaccac
     5521 tataggcgtt aagctctatc cttttgtaat ggttataaac agctacagcc aatatcttct
     5581 ttgccctttc ctggcttatt acgtactcgt ctaagaatgc cttaatctga taaggggttg
     5641 gaagagtcga atatcgtctt cttagatggc cctcttttgc ctcttcctcc gctattattt
     5701 cattacataa ctcaatacac tcattgcaga tataaacagt aggacccgca atcagcttcc
     5761 taacctcttt ctgacttttt ccgcagagtg agcaataaag gtttccgtct ctgccttctc
     5821 gtctggtagt cactttgtcg cttcctccct tttagtaatt atagcatcaa ctatgccgta
     5881 ctctaaggca tccttagcag taagaaaaaa atccctttct gtgtcccttg ctattctttc
     5941 aagaggttgt cctatatgct ttgcaagtat tcgatttata tcctctcgaa ccctaaggat
     6001 ctcttttgcg tgaatctcaa tgtctgtagc ctgtccctca acgccaccta agacctgatg
     6061 aagaagtata cgggcatgag gaagtgcata tctttttccc ttgataccag cggcaagaag
     6121 tacagccgcc atactggcag cctgaccgat acagattgtt gctacatctg gctttatgta
     6181 ttgcatagta tcataaatag caagcccagc agtgacgtgg ccacctggag aattcacata
     6241 aacataaata tccttttcgg gactttcaga ttcaagaaaa agcaactgag caattaccgt
     6301 atccgcaacc ccatctgtaa ttggcgatcc tacaaagact atcctgtctt ttaaaagacg
     6361 ggagaatata tcataagccc tttctcccct gttggtctgc tctattacaa taggcacata
     6421 tgaggacatt tcgaattttt agtatactga ttttagccct ctttgtcaat ttgagtttcc
     6481 tctttaacat cggatttttc gattaaaaag ttaagaacct tatcttcaac taaactcgct
     6541 tttaagctat caatcaaatt attcttttca tatatctctc tcaccttttg aaatggaacc
     6601 gctgcagttt ttgagatctc agtgagttta ttgtctacct catcctcgtc tacctgaact
     6661 ccttccttcc tagcgattgc acctaatatt atcgaggcct ttacattctg ctcggctcta
     6721 tttctaaaat ttccttctac ctcttcattc aacgctggaa gcttaagacc ctgtctttca
     6781 aggttgagtg agaactcacg cttaagacgt agggtttcat tctcaatcaa gcttggtgga
     6841 acatcaagag ggtttctccc aagaagaacc ttaataagct cccctttaag tctttcctcc
     6901 gattccttat taaacctttt ttctaaatcc tcccgaatct tcttctttaa tccctctaga
     6961 ttctcctcgc ctaaatcctt tgagaaatca tcatcgagct caggaagtat tttctcaata
     7021 atctccttca ccgttactgt aaattttact gtcttcccag caacatcctt catttggaaa
     7081 tcctctgcgt atgtaatttc aaattccttt tcttcaccct ttcttatgcc gattagatta
     7141 tcttcaaact cagctattag ttttttgtca cccactataa cttgggcatc ctttgcttta
     7201 aggtctttaa taggttttcc gtcaacagtc ccaacataat caattatcaa gtaatctcct
     7261 gttctaacct ccctatcctc tccgataggt ttagcttgag cacctcgttc cctaatttga
     7321 tttagaaccc gctctatatc ctcttctttc acctcgcgta tttgcttctt tagctcgatc
     7381 tcaatataat cctttacctc aaactcagga attatttcaa agactgctgt atagtgaaat
     7441 tctttatccc tttcaatctt gtcttttgtt attctcggtc tagatatggg tatgatagaa
     7501 acctcctcaa gagccttttc aaatgactga gatacaagcc ttgaggatac ctcctcaaat
     7561 atatagcccc cgtatgttgc ttctataacc cttctcggag ccttaccggg gcgaaacccc
     7621 ttaatttttg cttccctctc tagctcttta aacacttctt ctaactttcc tattacctcc
     7681 tctttaggta taaacacctc aacctttctc tctgtattac tgagtgattc aacgttgacc
     7741 ttcatcggat ggaactcctt ttatcttgcg taagaattta gctaagaata aacttgtatc
     7801 acgtcaatat aatgatgcat gatgcacgat acatgattca tgataatctt gcattctgca
     7861 tcttttatcc tgcatccaac atattttcgt ttttgccttg ctgccttagt ggcaaatagt
     7921 taatgcaact catgcgagag gggggagttg aacccccacg ggttgtgccc actggatcct
     7981 aagtccagcg cgtctgccag ttccgccact ctcgcttatg gttccctgat ccccgacatg
     8041 atcggggtta ccacagactc cggaatgggc tgcgagggat tcgaacccac gacccagtga
     8101 ttaagagtca cttgctctac caagctgagc tagcagccca cttaataatt ttaataaatt
     8161 acaactaata attttctcta cgctgctaaa gtaattacaa ttagaagact tttaccttat
     8221 tacttctatt attggcagaa tttattataa atacgttatt attaaaatca actttcccca
     8281 ttttctctgt ttcctttctt tatcaagcat caaggtatat tttgttaagt tgttactgat
     8341 tataatagtt cttagaaatg cttctattta tctaaagctt caagtgctga ttacttcttg
     8401 tgtatttatc ttgaaacagt ccattcaata atcgctgcga cgtctgccgg actttttgag
     8461 actgatcatt gccacttacc aaaaccacca tgttcgttta ccgcctttac ccattcatcc
     8521 agaaattcac gctttgtttt atcctgttgg gtttcttgtc ccttcgtttc taggacaaga
     8581 aaatcttcct atgttaaatc tgattcgaat tctcttgcat gtctggctgc aataagtata
     8641 tcaagaagat atgcgtcatc tcgccacata tatgacctct aaatgatcga ggatatgttt
     8701 tctgcgaata taattttcgc tttgctcaac agatttcttt tcaaccaggt ctacttcacg
     8761 cccaaatatt tctttcagtt catcctccat ttgaat
//
LOCUS       DATDRL010000319         2361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_647961, whole genome shotgun sequence.
ACCESSION   DATDRL010000319 DATDRL010000000
VERSION     DATDRL010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647961"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /gene="pilM"
                     /locus_tag="VI935_10665"
     CDS             <1..553
                     /gene="pilM"
                     /locus_tag="VI935_10665"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HLE26092.1"
                     /translation="FALENMYGINYEVKEGETVALINIGASLTNINILQKGMVAFTRD
                     VSIGGNKYTESIQKDMGLSYEDAERAKKGESIEGVDSFTLDSVIENVSVEIVNEITRS
                     FGYFKTASGNEKIDKIMLSGGSSRIRNLDNFLQSHLDIPVEVINPFRKIEIPPEFDSD
                     YLRDMAPMAAVAVGLGIRRLNDK"
     gene            550..1110
                     /locus_tag="VI935_10670"
     CDS             550..1110
                     /locus_tag="VI935_10670"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HLE26093.1"
                     /translation="MIKINLLPTKKVKRTITLRVGIKPTMTLFFAAISVVLLLEGLAW
                     YWLNSSLSSLTEEKRSLEIRLKEVKEKVKEVENYERDKKIYSEKIAIIQNLKKNQKGP
                     VRVLDELSSMLPERVWLVSLREIGKDVTIIGSGMTNDDIVKYVNNLKSSKLFKNVQLI
                     ESRQIVDSGIPIYNFSLTFSLNLDTV"
     gene            1114..1731
                     /gene="pilO"
                     /locus_tag="VI935_10675"
     CDS             1114..1731
                     /gene="pilO"
                     /locus_tag="VI935_10675"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HLE26094.1"
                     /translation="MNLESLENLPRSQKILMVSLLAIIIVGGFVYFVFIPQKVAIDAL
                     KNSINEINRQINNDEVKVRRLDQLKMENQQLQLELTRLQTQLPAEQEVSGLLKQISDL
                     SVESGLQIKLWKPAGRKNDEAGLYIEIPVDVEVAGGYHALATFFDRVSKLPRIVNITN
                     LSMEGTKVVSDSVFIQNKFVATTFAAVSESAEPPKSTQTKSTQKK"
     gene            1926..2321
                     /locus_tag="VI935_10680"
     CDS             1926..2321
                     /locus_tag="VI935_10680"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HLE26095.1"
                     /translation="MSSISIGGTSQETETAYTYRVEGRRDPFKSLLLGLKEKKSSGLT
                     PLQQRSLSELKVIGIMWKDQGHTAMIETPDGKGYLVKEGALVGPDGGFIQEITEDSIL
                     IEEGYTDYYGKKRSKKTVLRLHAKEEGGG"
BASE COUNT          817 a          364 c          538 g          642 t
ORIGIN      
        1 atttgcgctt gaaaatatgt atggaattaa ttatgaggtg aaggagggag aaaccgttgc
       61 attgatcaat attggagcca gtcttaccaa tattaatatt ctccagaaag gcatggttgc
      121 ctttacacgg gatgtttcta tcggtggcaa taaatataca gagtctattc agaaagacat
      181 gggattatcc tatgaagatg ctgaaagggc caagaaaggg gaaagtatag aaggggttga
      241 ttctttcacc ttagactctg tcattgagaa tgtttcagta gagattgtca atgagattac
      301 aagatccttt ggatatttca agactgcttc gggtaacgaa aaaatagata agattatgct
      361 gagtggcggt tcatccagga taagaaatct tgataatttt ttacagagtc atcttgatat
      421 tcctgttgag gttatcaatc cattcaggaa aatagagata cccccggagt ttgacagtga
      481 ctacctaagg gatatggctc ccatggcggc agtcgcagtt gggttgggaa taagaaggtt
      541 gaacgataaa tgattaaaat aaacctcctg cctacaaaaa aggtaaagag aaccattact
      601 cttagggtag ggattaaacc tacgatgaca ctattttttg ccgccatctc agtggttcta
      661 ttattagaag gccttgcatg gtattggctt aacagcagcc tatcctctct cacagaagaa
      721 aagaggagtc ttgagatcag acttaaagag gttaaggaaa aggtcaaaga agtagagaat
      781 tatgagagag ataaaaagat atatagtgag aagatagcca ttatacaaaa cctaaagaaa
      841 aatcagaaag gacctgtccg tgtattagat gaactcagta gtatgttgcc tgaaagggtt
      901 tggcttgttt cccttcgaga gatagggaag gatgtaacta ttataggatc cgggatgacg
      961 aatgatgata tagtaaagta tgtaaataat cttaaatcct caaaattatt taaaaatgtc
     1021 caattaatag aatcgagaca gatagttgat tctggtatcc caatctacaa ttttagttta
     1081 acatttagtc ttaatttgga tacggtgtag ataatgaatc ttgagtcgtt agaaaatttg
     1141 ccacgttcac aaaagattct gatggtatcc ttgctggcaa tcataatagt tggtggtttt
     1201 gtttactttg tatttatacc ccaaaaggtc gctatagatg ctttaaagaa tagcatcaat
     1261 gaaatcaatc ggcagatcaa taacgatgaa gtaaaagtga ggagacttga ccaactaaaa
     1321 atggaaaatc aacaactcca attggagcta accaggcttc agacacagct tcccgcagag
     1381 caggaggtat cggggttact caaacagatt tcagatctta gtgtggaatc agggttacaa
     1441 ataaaattat ggaaaccagc tggaagaaaa aatgacgaag cgggtctcta tatagaaatt
     1501 cctgttgacg tcgaggtggc aggaggttat catgcacttg caaccttctt tgatcgtgta
     1561 agcaaactcc caagaattgt aaatatcact aatttaagta tggaagggac taaagtagtt
     1621 agtgatagcg ttttcatcca gaacaagttt gttgctacaa catttgcagc ggttagtgaa
     1681 agcgctgaac ctccaaagag cacacaaaca aagagtacgc aaaaaaaata ataatgatat
     1741 cctgtcacat caaaaaagaa atagggcaaa ctataatgtt agtcttcgat gagataagat
     1801 tgacaacgat atttttaatt atcttattaa caattagtag ttgtggggag caggcgactc
     1861 aaccattaaa atctctggta aaacccaaaa caaacaagac tattaataaa cagcctactg
     1921 cagatgtgtc ttcgatatcg atagggggga catcacaaga gacggaaacc gcttacactt
     1981 atagggttga agggagaaga gatcctttta aatcccttct ccttggttta aaggagaaaa
     2041 agtcatcggg attgactcca cttcaacaga gaagtttgtc agagctaaag gtgattggaa
     2101 ttatgtggaa agatcagggg catactgcaa tgatagagac acctgatggc aaaggttact
     2161 tggtgaaaga aggtgcactg gtaggtcccg atggagggtt tattcaagaa atcactgagg
     2221 actcaatact aatagaagaa ggatacacag attattatgg gaaaaagagg tctaagaaga
     2281 cagtgttgcg gcttcatgct aaagaggagg gtggtggatg aagatattga attttaggtt
     2341 gattaggtac cttatcctta t
//
LOCUS       DATDRL010000320         6748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2491063, whole genome shotgun sequence.
ACCESSION   DATDRL010000320 DATDRL010000000
VERSION     DATDRL010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2491063"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VI935_10685"
     CDS             <1..344
                     /locus_tag="VI935_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26096.1"
                     /translation="EVAKLTVSKNIGFQKVERGSTEGDPTQQTLVLVGEITGYESGKS
                     FKKEGGALKFGEASLTVHVWLLDKNTGDDITDGEVSGLSTFGFLRSGFITKGVYEAIA
                     QEIVKFISKNY"
     gene            626..1672
                     /locus_tag="VI935_10690"
     CDS             626..1672
                     /locus_tag="VI935_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE26097.1"
                     /translation="MRKKISGLFMLLFLSVFILGAQDFAQSKEKPKEDNSQMQNSNAA
                     PYYEKGRTAFLQFTPKGFQDAIVYFNQAIEVDPRYAPAYAGLGEVYSFIGFYKLEVKE
                     DYEEFYNKSYENIIKALKYGTNTKETQRALALNYLHLKRSKVAEVAANRALAKDPNDA
                     ESYYILWAASGRDPDSPYVVKALGLDPNLVMAHLDLGKAYLFKRGNYAKATEQFKKAV
                     ELADSPQLHNFLGTSLRTQGYLSNSVTEYLKAIELDPTYAPAYMNLGITYYSMNKYQD
                     SIAQEKKAISLNQNNPEPYYHLARAYDLANSPKEAIENYRIFLKLTSGKEIYTNLTTN
                     AKTSIEKLHASCNC"
     gene            2100..2438
                     /locus_tag="VI935_10695"
     CDS             2100..2438
                     /locus_tag="VI935_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26098.1"
                     /translation="MDKLTTRLDLVDLSFGLESNASLWSAVFKGFDEEVTLISRFEGR
                     EKEGIKTLQRFGTILIGNYDNFQVELTNSGIIIKKTLQTNDPSSIHEWSELMHNLKEE
                     VVNIIERSRD"
     gene            complement(2735..3136)
                     /locus_tag="VI935_10700"
     CDS             complement(2735..3136)
                     /locus_tag="VI935_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLE26099.1"
                     /translation="MIKGIDHVAIVVWNMDRSIKFYNEVLGLRIHHDGRKEGGSKKSF
                     LGNKSETLVALTEDENRGKEGNRIVEGVAHIAFRVEDVEKASKLLKERGVEFIEEKLG
                     KDGKRKSYHFLDPDGLELEIYGRTGQIIPPY"
     gene            3575..4237
                     /locus_tag="VI935_10705"
     CDS             3575..4237
                     /locus_tag="VI935_10705"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HLE26100.1"
                     /translation="MAKIHSARKFLEELREEFQPLRKKILTHAFVGSVEKGKASVEKL
                     KFFVEQQFHIVSGDFRNLALFIAFSPNQRIRDFFFELIQGERSALDNLFKMAKALGIG
                     DQELEASEPHAGSLAFTNYFTRLATYGTEGEIASAIIIDFEVWGENCSRISKGLKRNY
                     NFTSEDTKFLDGFYPITSQFYDKITKIIGEYARIEKDIKKMRIATRLGLDYELMFWDT
                     ML"
     gene            complement(4315..5109)
                     /locus_tag="VI935_10710"
     CDS             complement(4315..5109)
                     /locus_tag="VI935_10710"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HLE26101.1"
                     /translation="MKSLKSESIKKLLKTETIGKIIYSFEEVGSTNEVASELARNGAS
                     EGTTIIADSQSKGKGRLQRRWISPPGVNLYMSVIFRPAIAAKDAPLLTLVASIALAET
                     IKGKEPDTLIKWPNDILVKGKKVAGVLTGMQPIGNKVDFIIVGIGVNINMTREMMERE
                     MGEVSEIATSLREALGYDIKREKFATDLIEELEIWYHKFLKEGKSRIIKEWMERWGAI
                     NRKVIVRFDGKVIEGIASRIDENGHLLVKKDDGTTEKIIAGDVVLL"
     gene            5413..6378
                     /locus_tag="VI935_10715"
     CDS             5413..6378
                     /locus_tag="VI935_10715"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HLE26102.1"
                     /translation="MASITDVPGVRVGHSSDFEAITGCTVILFDNEATGAIDLRGGGT
                     STRQIDPLLLYHSYGKVHGIFLTGGSAYGLDAAGGVMRYLEEQGIGLDVGYGHIVPSV
                     PTAVVFDLGLGNGKVRPDQSMAYSACLNASSSPTEEGSIGVGTGASIGKLLGIKQATK
                     GGIGSTSYNLGNGIVVGVLVVVNAFGDIVDPKSGGIIAGVRNSIDGKEFLGSVKLFKS
                     GVTQKAEPFENTTLAVVATNVKLSRSRLVRVARIGQTGITRVVSPAHTTADGDLVFAI
                     SCGDLPADVNLVGIVASELISEAIIKAVRAAKGMGGIPSWKEIQK"
     gene            complement(6526..>6748)
                     /locus_tag="VI935_10720"
     CDS             complement(6526..>6748)
                     /locus_tag="VI935_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HLE26103.1"
                     /translation="RFSMVLSLGIASIFFWHTLINVGMVTGLLPVMGVPLPLLSYGGS
                     SLITAMLGVGIVLGIRMRKFPLTKEDIAL"
BASE COUNT         2094 a         1387 c         1370 g         1897 t
ORIGIN      
        1 atgaggtagc aaagttaact gtttctaaga acattggctt ccaaaaagtg gagcgtggtt
       61 caactgaagg cgaccccact cagcaaaccc ttgtcctcgt tggtgaaata acaggatatg
      121 aatcgggaaa aagctttaag aaggaagggg gggcgttaaa atttggcgag gcttctctca
      181 cagttcacgt atggctttta gataaaaaca caggagatga catcacggat ggtgaagtaa
      241 gcggtttgag tacgtttgga tttctaagaa gcggatttat tacaaaaggt gtttatgaag
      301 ctatagcaca agagattgta aaatttattt ctaagaatta ttgataagaa ggttaaatta
      361 tagtaaccta gctttccaag ttgtcattgc gagcacactc gtttcactca gtgtaaactc
      421 cgcgaagcaa tttgacatga agtgtcattc cgaatatttc gccttggctc agtacaggcg
      481 cagtgaggaa tctcaactag attccttcgt cgctttgctc ctcgcaatga cagatgtaag
      541 gtcttgctct ttgtaatgaa aaaataaatt atggattatt ttctgacaca caataaatat
      601 aaaggtgcaa ggagggacga cgtctatgag aaaaaaaata tctggtctat tcatgcttct
      661 attcctttca gtgtttattt taggggctca ggattttgct caatccaagg aaaagcccaa
      721 agaagataat tcacagatgc aaaactcaaa tgcggctcca tactatgaga aaggaagaac
      781 cgcgtttctg caatttacgc ctaaaggatt tcaagatgcg atcgtatact ttaaccaagc
      841 aatcgaagta gacccaaggt acgctccggc ttacgcaggc cttggagaag tttattcatt
      901 tattggcttc tataagttgg aggtgaaaga agactacgag gaattctata ataagtccta
      961 cgaaaacatc attaaagccc taaagtacgg cactaatact aaagagacac aacgcgcctt
     1021 ggctctaaat taccttcatc ttaagcgatc aaaggttgcg gaagtagctg ctaaccgcgc
     1081 attagcgaaa gaccctaacg acgcagaatc atactatata ctttgggccg catctggcag
     1141 agatccagat agcccgtacg ttgtaaaagc acttgggtta gatccgaacc tcgttatggc
     1201 tcacctagat ttaggaaaag cctacttatt taagagaggt aattatgcaa aggcaacaga
     1261 acaatttaaa aaggcagtcg aactcgcaga ttcgcctcaa ttgcacaatt tcctaggaac
     1321 ctcgcttcgt acacaaggat acctcagcaa ctcagtaaca gaatatctaa aagcaataga
     1381 gctcgatcca acttatgctc ctgcctacat gaatctgggg atcacttatt attcaatgaa
     1441 taaataccaa gattctattg cccaagagaa aaaagcaatc tcccttaatc aaaataatcc
     1501 tgaaccttac taccatcttg cacgtgcata tgaccttgcc aatagcccga aggaggcgat
     1561 agagaattat aggatttttt taaagcttac atctggaaaa gaaatatata ctaacctcac
     1621 tacgaacgcc aagacgagta ttgaaaaact acatgcaagc tgtaattgtt aacttttatt
     1681 tatgtacttt ttctctatgt tcaatgaaga accccgtagc tgcaaggcac cggcggttaa
     1741 aaaacccttg gtgctgcgag gagcaagtga gccgaagccc agagaaaagc gaaggggaca
     1801 aagcaaactg acgcatagcg tcatcctgaa ggagcaaagc gactgaagga tctcctgatt
     1861 atcttgggat tcttcgcgga gtttatcctg aggtatcgaa ggactcagga cgacactcgg
     1921 tgtcggattc ctcacggagc ctgtactgag ccaaggcgaa gtattcggaa tgacacttca
     1981 tgtcaaattg cttcgcagag tatacactgc gtgaaacgaa tgtgctcgca atgacagaga
     2041 aatccctgta gcaagataca gaaaattata aagctaaaac ttaatcttct ttagtaataa
     2101 tggataagtt aacaacacgg ttggatttgg ttgacctttc ttttgggcta gagtcaaatg
     2161 caagcctctg gagcgcagtg tttaagggat ttgatgaaga ggttaccttg atctcaagat
     2221 tcgaaggaag agaaaaagag ggtattaaga cacttcagag atttggaacc atactaattg
     2281 gaaactatga taatttccaa gtcgagttaa caaactcagg aattataatt aagaaaaccc
     2341 tccaaacaaa cgacccttct tctattcatg aatggtcaga gctaatgcat aatcttaaag
     2401 aagaagtggt taatataata gaaagatcca gggattgatt tttttgatac ttggcacttt
     2461 attcggtttt ttgctttatt gttgagtctc ttacaactta tcagtaaatg aagaaccctg
     2521 cagcgagctg caggacatca gcgtttaaac agaagactcg ctcactttgt acaagcaggg
     2581 caaggatact gttgtcatag caacgacgtg gaaacccttt agcaagctac aatgaatttg
     2641 ctagttaaaa agcataaatg aaaacagtat ttgcaaagtc taagacaaaa aatgtacccg
     2701 cttccccaat agcctcaatt taaaacattt ttcatcaata tggtggtatt atttgaccgg
     2761 ttctaccata tatctctaac tcaagcccat ctgggtctag gaagtgatat gattttctct
     2821 ttccatcttt acccaatttc tcctctataa attctactcc tctttcttta aggagtttgc
     2881 ttgccttctc cacatcctcc accctaaaag ctatatgtgc cacgccctca acaatccgat
     2941 ttccttcttt acctcgattt tcatcctctg ttaaagcaac taaggtctct gatttattgc
     3001 ccagaaagga ttttttgcta ccaccttcct ttcgtccatc atgatgtatc ctgagcccta
     3061 aaacctcatt gtaaaacttg atggatctat ccatgttcca aacaacaatt gcgacatggt
     3121 ctattccttt aatcattgta tccactatca tgtgctttta accctctata ataccctaat
     3181 ttaaataatg aacttatacc aatttgtatt agatgaatta ataaataatc gaatgaactt
     3241 atgcaaactg gatatcttag gaaaaggtta tgtcatcaat gtttgcctta aatttataca
     3301 ctctacccag agtccacatg ttataattta attaataacc cttcagcaag ctgcgggcat
     3361 ggtcttaata ttaaacgccg caactgtcat tgcgaggaac aacgtgacga agcaatctaa
     3421 aatgagagat tgcttcacgg agcctgcact gaacaaagtg aacgtgttcg caatgacaat
     3481 acagcattat ggaaacacct tagcaagcta cagggaatta ccaagtcaaa attaacggtt
     3541 agaaactgaa caacttcaaa aataaggtta atcaatggca aagatacact ctgcaagaaa
     3601 attccttgag gaactaaggg aagaatttca gcctttaaga aaaaagattc ttactcatgc
     3661 ttttgttggt agtgtagaaa aagggaaggc ttccgtcgaa aaactaaagt tctttgtaga
     3721 acagcaattc catatcgtaa gcggggattt cagaaatctg gctctattca ttgccttttc
     3781 ccctaatcag cggataagag attttttctt tgagttgata caaggagagc gtagtgcctt
     3841 agataacctc tttaaaatgg caaaggctct aggtattgga gatcaagaac tcgaggcttc
     3901 cgaaccccat gcaggctcac tggcttttac aaattatttt acacgtctag caacctatgg
     3961 aaccgaagga gagatagctt cagcaataat catagatttt gaagtctggg gcgagaactg
     4021 tagcagaata agcaaaggac ttaaaagaaa ctataatttt acctctgaag atacaaaatt
     4081 tttggacgga ttttatccga ttacctctca gttctacgat aaaataacga agataatagg
     4141 ggagtacgct agaatagaga aagatattaa gaagatgaga atcgcaacta gattaggtct
     4201 cgattatgag ctgatgttct gggatacaat gctatagggg cagacttcgt cgtgagctca
     4261 gtcgaacgac cccccgtgtc tgctctaata acgcgcaatc acagaggatt gcccttacaa
     4321 aagaaccaca tcccctgcga ttatcttctc tgttgttcca tcatcctttt ttacaagtaa
     4381 atgcccgttc tcgtcaatac gagacgcgat tccttcaatc accttcccat cgaacctgac
     4441 tattacttta cggtttatcg ctccccacct ttccatccat tcctttatga ttcgagactt
     4501 tccctctttg agaaacttat gataccaaat ctcaagctcc tcaatcaaat cagtggcaaa
     4561 tttttctctc ttaatgtcgt atcctaacgc ctccctaaga gaggttgcta tctctgaaac
     4621 ctctcccatt tctctttcca tcatttccct agtcatgttt atatttaccc cgatccccac
     4681 aataataaaa tcaactttgt ttccaatagg ttgcattcct gtcaaaacac ccgccacctt
     4741 tttccctttt acaagtatat cattgggcca ttttatgagt gtgtcaggtt ctttgccctt
     4801 gattgtttca gccaaagcga ttgaagcaac cagagtaaga agaggagcat cttttgctgc
     4861 aattgcaggg cgaaaaatca ccgacatata gaggttcact cctggggggg aaatccatct
     4921 cctttgaagt cttcctttcc ccttggactg agaatcagca attatggtag tcccctcaga
     4981 ggcaccgttt cttgcaagct cagatgcaac ttcgttggtg gagccaacct cttcaaagga
     5041 gtagattatt ttcccaattg tttcagtttt aagaagtttt tttatcgatt cgctcttgag
     5101 tgatttcata aaagaaccct acctgcaaaa tcttacctgt tcttttattc taagaccaga
     5161 tttttctaaa gtaacctttt ttgatcttca tcccgagctt atgagcccat gcccagagac
     5221 aagcgaagga ggaagcaatc tcccctttct taatatttcg gtgatgatgt aatattgagt
     5281 ttgcttcggc tgatttcatc ggcctcgcaa tgacaaaaaa aattcgaacc gccttgggga
     5341 tttccctacg attgcatgac tatatgtaaa gtttacttgg aaaccaatga taatgaattt
     5401 aaaatgtaaa cgatggcctc aatcactgat gttccgggag taagggttgg acactcatca
     5461 gattttgaag caataactgg ttgcacggtc attttattcg ataacgaggc aaccggggct
     5521 atagacctaa gaggtggcgg gacgagcaca cgccaaatag acccgcttct tctataccat
     5581 tcatatggta aggttcatgg aatcttcctt acagggggta gcgcttacgg attagatgca
     5641 gccggcggag taatgaggta tcttgaagag cagggaatag gactcgatgt cggttacggc
     5701 catattgtac cctcagttcc tactgctgtc gtctttgacc ttgggcttgg taatggaaaa
     5761 gtgaggccag accaatctat ggcatatagc gcatgtctaa atgcatcgtc gtcacctacc
     5821 gaagaaggta gcatcggagt gggaacaggg gcatctatcg gaaagcttct cggcatcaag
     5881 caagcaacaa agggtggaat tgggagcacg agttacaact tgggaaacgg catagttgtt
     5941 ggagtgcttg ttgttgtaaa tgcctttggt gatatagttg atcctaaaag tggggggatt
     6001 attgcaggtg tgagaaactc cattgacgga aaagaattct taggttcagt aaaattgttt
     6061 aagagcgggg tgacacaaaa agcagaacca tttgaaaata cgactcttgc agtggttgcc
     6121 acgaatgtaa aactttcaag gtctcgccta gtgcgggttg caagaattgg gcagactggg
     6181 attacaaggg tagtttctcc cgctcatact acagcggatg gtgatttagt atttgccata
     6241 tcttgcggag acttaccagc cgatgttaat ttagttggaa tcgtcgcttc tgagcttatt
     6301 tctgaggcta taataaaagc cgtaagagca gcaaaaggca tgggtgggat tccaagctgg
     6361 aaggaaattc aaaaataagt agccacgaat aaaaatgata cacgatgcat gatgcatgat
     6421 gagtaattca tgagagtttc acatgcatcc tgcatcgtgt atcctgcatc ctgattcgtg
     6481 tccatttgtg tcaatttgta acaagattga gagaaatcca ttctactaaa gtgcaatatc
     6541 ttcctttgta agtgggaatt ttctcatcct tattcctaaa acaattccta caccaagcat
     6601 ggctgtgatc aaagaagagc cgccataact aagaagcgga agtggaacac ccataactgg
     6661 cagtaaaccc gtaaccatgc ctacattaat tagggtatgc cagaaaaata tcgaagctat
     6721 acctaaagat aagaccattg aaaatcta
//
LOCUS       DATDRL010000321         3945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1703328, whole genome shotgun sequence.
ACCESSION   DATDRL010000321 DATDRL010000000
VERSION     DATDRL010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703328"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /gene="mce"
                     /locus_tag="VI935_10725"
     CDS             complement(<1..382)
                     /gene="mce"
                     /locus_tag="VI935_10725"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HLE26104.1"
                     /translation="MKSGITGRLYHVAIAVRNIDEVVKLYETALGLKVTHVEVVGEQG
                     VKAAMLEPEGGGPAIELLEPLSDNSPISKFLEKRGEGIHHICFLVDDIESALERLKKE
                     GVQLVDQSPRPGVHETRVAFIHPKA"
     gene            810..1835
                     /locus_tag="VI935_10730"
     CDS             810..1835
                     /locus_tag="VI935_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HLE26105.1"
                     /translation="MSHANIHKVTLIPGDGIGPEVTDAARRIIQATGVRIEWEVVKAG
                     YHALEKCKTVLPDIVFDSIRRNKVALKGPVGTPIGEGFRSVSVTIRKTLDLFANLRPV
                     KSFPGVKSRYECIDIVVIRENTEDLYSGLEHEVAPGVVETLKVITERASLRIANFAFR
                     FAKRRGRKKVTAIHKANIMKLSDGLFLDCVRRVSRDYPVIGYEEVIVDNACMQLVLNP
                     NRFDVLLTENLYGDIVSEICAGLVGGLGFAPGANIGDNYAVFEAAHGSAPDIAGKNLA
                     NPIAITLSGALLLKYIGENREAKIIEQAISKVLARGEVFTKDMGGNATTTELRDAIIN
                     EIKAKNH"
     gene            complement(2006..2431)
                     /locus_tag="VI935_10735"
     CDS             complement(2006..2431)
                     /locus_tag="VI935_10735"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HLE26106.1"
                     /translation="MKDKLQAGMFIEKKIQTTPEMAASRFHESSPRVLSSPSLLTFIQ
                     TTCADLLAPFLDTGEMAVTIKIEMNHLASTPIGMPVTIRADIIRVDGRRIYFKVEAFD
                     EIEKTAQGYNDMFIIDEERFERGIKRKWEKTQMQASEVK"
     gene            complement(2891..3100)
                     /locus_tag="VI935_10740"
     CDS             complement(2891..3100)
                     /locus_tag="VI935_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26107.1"
                     /translation="MRTKVLAKKIKQDADYIYLKIRRDTLEAFCDTAGLYRQEFLDLL
                     DESEKDHRAGRVTKRKSLYELITEI"
BASE COUNT         1158 a          769 c          852 g         1166 t
ORIGIN      
        1 aagcctttgg atggataaaa gcaacccttg tttcatggac accgggacgg ggcgactgat
       61 ctactagttg aacgccttct ttttttagcc tttcaagtgc cgattcaata tcgtctacaa
      121 gaaaacagat gtggtgaatg ccttcccctc tcttttctaa gaactttgag atgggggagt
      181 tatcgcttaa tggctcaaga agttctatag caggcccgcc accttcgggc tctagcattg
      241 ctgctttcac tccctgctct ccaactactt ccacgtgagt gactttaaga ccgagtgccg
      301 tttcgtatag ctttaccact tcgtcaatgt tcctaacagc aattgctacg tgatagagtc
      361 tccctgttat tccgcttttc attgtcgagt atcctcttgc ctgtcattgc gaggagtgaa
      421 acgacgaagc aatccaaaca acagaattgc ttcccctttt gcggagttta ccctgaggta
      481 tcgaagggct ctgggctttg gcacacacgc tcgcaatgac aattttggaa agtcatgcac
      541 ttaactataa ttggcacttt gagaaaattc aaatttaaag aacgtgtggc cgacgcaagg
      601 acatatttct agcggaatcc ttccttcgat aaaactcagg acaggttagg attccattcc
      661 cgattaagac attcgggaat gacatttatg gaggtctaaa gacctccgct acatttaatt
      721 ttcactcttg aaaagttata tcgaactttg gattaccaag ataaggggtt aaaattataa
      781 aaaatatata taaagaggag tgtattaata tgtcacatgc caatatacat aaggttacac
      841 ttatacctgg cgacggaatc ggacctgagg ttacagatgc tgcaaggagg attatacaag
      901 ccacaggtgt aaggatagaa tgggaagtag taaaagccgg ttatcatgca ttagagaaat
      961 gtaaaaccgt tctacctgat attgtattcg attcaatcag aaggaataag gttgccctta
     1021 aaggtcctgt cggcacgcct attggagagg ggtttagaag tgtaagtgtg acaataagaa
     1081 agacacttga tttgtttgcg aatctacgac cggtgaaaag ttttcctggt gttaaatcta
     1141 ggtatgagtg tattgatatt gttgtaatta gagaaaacac tgaggacctt tattcagggc
     1201 ttgaacatga ggttgctcct ggtgttgttg aaactttaaa ggttattacc gaaagggcat
     1261 ctctgagaat agccaatttt gcctttcgct ttgcaaaaag gcgtgggagg aaaaaggtaa
     1321 ccgctataca taaagcaaac ataatgaaat taagcgatgg gctatttctc gattgtgtta
     1381 ggagggtatc aagagattac ccagtgatcg gttatgaaga ggtgatagtt gataacgctt
     1441 gtatgcagct tgttctaaat ccaaacaggt ttgatgtcct tctcactgaa aatttatatg
     1501 gtgatattgt ctctgaaatc tgtgcgggac tagtgggggg actaggattt gctccaggtg
     1561 ctaatatagg agataactac gcagtttttg aagcagcgca tggcagtgcg ccagatattg
     1621 cagggaaaaa cctagcaaat cctatagcga taacgctttc tggggcgctt cttctaaaat
     1681 atatcggaga aaatagagag gcaaaaatta tagaacaggc aataagtaag gtattagcaa
     1741 gaggagaagt atttactaag gatatgggtg gtaatgctac tactacagaa ctaagggatg
     1801 caattataaa tgagattaaa gcaaaaaatc attgattaaa agatgcatga tacatgatgc
     1861 acgattcatg atttatcttg catcctgcat cttgcatcct tcttatcttc aatcgcgtcc
     1921 attagtgtaa attggcttcg ccgaaatgcc cgaccctcaa gcctacattt tcgtggctga
     1981 ataattacag ttttaaaccc tttatctact taacctcgct agcctgcatt tgcgtcttct
     2041 cccattttct tttgattcct ctttcaaatc tttcttcgtc aattatgaac atatcattat
     2101 aaccttgtgc ggttttttcg atctcgtcaa aggcttctac cttaaagtag attcttctcc
     2161 cgtccaccct tataatatca gccctaattg ttacgggcat acctataggt gttgaggcaa
     2221 ggtgattcat ttcaatcttt attgttaccg ccatctcacc cgtgtcaaga aagggtgcga
     2281 gcaggtctgc acatgtagtc tgaatgaagg taagaagaga cggcgatgaa agcacacgcg
     2341 gagagctttc atgaaaacgc gaagccgcca tctctggtgt tgtctgaatc ttcttttcga
     2401 tgaacattcc cgcctgaagc ttatccttca tgactcctcc ttgatttggg taatgccaat
     2461 tggatttttc gattgttgca taatgaataa tgtcattgcg agaaatgaaa tgacgatgta
     2521 atccgataca agtcatcatt ccgaaccatg tcctaagccg tagccctgag caaagcgaag
     2581 ggacagacga aggagcggca aggaaacctc aaacggattg cttcacttca ttcgcaatga
     2641 caaatcaggc ctgtcattgc gaggaactaa gtgacgaagc aatctgacac aaagtgtcat
     2701 tccgaaccct gtcctaagta aagtcgaagg aacggtgagg aatctcaaat agatcgcttt
     2761 cccttcactc tgggcttcgg ctcacactct cgcgatgaca actccttttt taatttacta
     2821 atgtttgata ctgaaattat atcaaatctt tgagcctctg aatatacaga gcaaaaatta
     2881 tggggaaaga ttatatttct gtgattagct catagagtga cttccgctta gtgacccggc
     2941 cggcgcgatg gtctttctct gattcatcca ataaatctaa aaactcctga cgatagagcc
     3001 ctgccgtatc acaaaacgcc tccagggtat ccctgcggat tttaagatag atgtaatcgg
     3061 catcttgctt gattttttta gccaagactt ttgttctcat agcacaatta tggtaaggct
     3121 cataattcca cttgtcaaac caaatcggac taaacacaga tcgtgtcatt ctgagcaaag
     3181 tgaagaatct cttgattgag cactgaaatg cttcacggag tttatcctga ggcatcgaag
     3241 ggttccgtat gaccaatgga tagtcattag ccggatttgg tatcaaatcg cgttctgctg
     3301 tgatataaat ggtttatcat aaaatcgtat gatttgtagt ataattaaga taactaaaat
     3361 ctttagttga aaggcgtctt atggtttcaa taactattta taaatacaac tcagacagaa
     3421 tagtggttac cttttcttat aaccctttaa atatcattgg gaaatgagat tacaaggagg
     3481 aagaatagtt caaccagagt ggtgaatggt ggtgaatggg gtcaggtctt gaaatataag
     3541 attttgtgtt gcggtggact tgataagaaa ccgagaatgc acaattaaag acctgacccc
     3601 aaaatttagc ctgagagcag catcggcctg cttcacctgc cactcctcat aactcttgga
     3661 tatatgcttc aaaaactgct tcctgtggtc gttgcttacg ggttgcgagt tgggtagatc
     3721 aaaaaaagca taacagtctg caacccattt gagataatag ggtacatatt ttgtcttaac
     3781 atgggcggtc ttggcgagat agttcatgct tctcacatta atcgacggga attgatcgta
     3841 caaaggcgtg atttcggtta atatctaata atattaaata taaaatttca tgattggagt
     3901 tcaataaatt tcaaggtgag caggacctct ggttattcag gtttc
//
LOCUS       DATDRL010000322         3409 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1969468, whole genome shotgun sequence.
ACCESSION   DATDRL010000322 DATDRL010000000
VERSION     DATDRL010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969468"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /gene="ptsP"
                     /locus_tag="VI935_10745"
     CDS             complement(<1..1331)
                     /gene="ptsP"
                     /locus_tag="VI935_10745"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HLE26108.1"
                     /translation="MRSETKGIAISPGVALGKVVLLNRSKMIVEKKSVEQNLLEAEKK
                     RFLVAVEKSKEQLITIKERFDPKETGDHLQILNFHLMMLEDELFCEDIIRTIEREKVN
                     AEWAITSVLSKKSEALRSVEDVYMRERLADIYYLGERILRNLHGVHDDIRDLDEDSIL
                     VAHDISPVDVVGFAKHHAIGLAIDTGGSASHSAIVARSLGIPTVMGLEDITLKVSPSD
                     LIFIDGFKGIVIVNPIEKELKEFKRRKKQYQALEKKLRSYATLPGRTLDGKQIKVNAN
                     IEMADEVDLAICYGAEGIGMYRTEFLLTSLAYFPSEEEQFEDYKKVISAFHSRPVTIR
                     TLDIGGDKFPIGMEPPKELNPALGLRGIRFSLWDEKIFDNQIRALLKASKFGKVRILI
                     PMVSSIEEIRQVKRIITRVSEEIDSRSFWEIGVMVETPSAALMASEIADEV"
     gene            complement(1335..1619)
                     /locus_tag="VI935_10750"
     CDS             complement(1335..1619)
                     /locus_tag="VI935_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HLE26109.1"
                     /translation="MGSVETLSKIFMIKNKLGLHARAAAMFVRLSNGFSSDIKILKDG
                     YEVNGKSILGILSLAAIKGSELKIVTTGEDAEEALGEIGKLIESGFGEEE"
     gene            complement(1911..2678)
                     /locus_tag="VI935_10755"
     CDS             complement(1911..2678)
                     /locus_tag="VI935_10755"
                     /inference="COORDINATES: protein motif:HMM:NF025490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HLE26110.1"
                     /translation="MRRSILIAVLLLFSCAKKTVVIDLGRINLPEVLTKVKEVNEGAR
                     SIKGIASITIKTPDRSVSFRQVTIVEESNLLHLEAIAPFGRTAGMVISDGKKTYVILP
                     EEKRVYDNYKEFDFSYIYPDLPVKITINNLVNLLLGRLPERQMNEGSEVQLSVKSNYI
                     ILTFLNSGKEDSVLWINPINYRIEKAKINLQGGVQTNCEFKDFKDIGSGVSFPTKIEL
                     KLDRFSISVKYDEEVEINGDLDKSLFKPNQPLARIEK"
     gene            complement(2734..>3409)
                     /locus_tag="VI935_10760"
     CDS             complement(2734..>3409)
                     /locus_tag="VI935_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE26111.1"
                     /translation="ALYNDALIQRAYLFEKMGRTDESLKMIKEALKHDPDNEQIVNYL
                     GALYRKLNRNEEGIKLYEDFVNTHPKSELIYYSLGVSYYFIKNEDKSILAMKKVLEIN
                     PDHADALNFIGYTYAESGENLDEAERLVKRAIEISPNKGYIIDSLGWIYYKKGNLDKA
                     TELLEKAAELTGDDPNIMEHLGDVYNDKGDRLKALEYYERGLELINKNQDLDEEEKEL
                     KTRLET"
BASE COUNT          886 a          750 c          576 g         1197 t
ORIGIN      
        1 tctacctcat cagctatctc ggatgccatt aacgctgcag aaggggtctc aaccatgact
       61 ccgatttccc agaaagatct cgaatcaatc tcctcggata cacgtgtgat tattcttttt
      121 acttgtctaa tttcctcaat gcttgacacc ataggaataa gaattctcac ttttccaaat
      181 ttacttgcct ttaagagtgc cctaatctga ttatcaaaga ttttttcatc ccatagggag
      241 aacctgattc cacgaagccc aagtgcgggg ttaagttctt ttggaggctc cattccgata
      301 gggaatttat caccaccaat gtcaagtgtt cttattgtta cagggcgtga atgaaatgca
      361 gagatgacct tcttgtaatc ttcaaactgc tcctcctctg acgggaaata agctaagctt
      421 gttaggagaa attcagtcct gtacattcct atcccttctg ctccataaca tatagcgaga
      481 tcaacttcgt ccgccatctc tatgttcgca tttactttaa tttgtttgcc atctaatgtt
      541 cttcctggaa gggttgcata gcttcttagt ttcttttcta gagcttgata ttgttttttt
      601 cttctcttaa attctttgag ctctttttct atcggattaa caataactat tcctttaaaa
      661 ccatctataa atattaggtc acttggagaa accttaagtg taatatcttc aagtcccata
      721 accgtaggga ttccaaggga tctcgccact atcgcactgt gtgatgctga gccacctgta
      781 tctattgcaa gccctatggc atggtgtttt gcaaacccga cgacatcaac aggcgatata
      841 tcatgcgcaa ccaggataga gtcctcatcc aggtctcgta tatcatcgtg tactccatga
      901 aggttacgga gaatcctttc tccaaggtaa tatatgtcag cgagtctctc tctcatgtaa
      961 acatcttcta cggaacgaag ggcctcgctt ttcttagata ggactgaggt tattgcccac
     1021 tcggcattaa ccttttccct ttcaatggtt ctgataatat cttcgcaaaa gagttcgtct
     1081 tcgagcatca taaggtgaaa atttaggatt tggagatggt ctccagtttc ttttggatca
     1141 aatctttctt ttattgtgat tagttgttct ttagatttct cgacggcaac caaaaatctc
     1201 tttttctcgg cctcaaggag attttgttcc acactctttt tttccacaat catctttgaa
     1261 cggttaagta agacgacctt tcctaaggca acaccgggag aaatggcgat tccctttgtc
     1321 tcagatctca tctcttattc ctcctcccca aaaccgcttt ctattagctt cccgatctca
     1381 ccaagtgcct cttcggcatc ctcacctgtg gttactatct tcaattcgct cccctttatt
     1441 gcagcaagag atagaatccc tagtatgctt ttaccgttca cctcatagcc atcttttaat
     1501 atctttatat ctgaagagaa tccatttgag agacggacaa acatagcagc cgcacgggca
     1561 tgaagcccaa gcttattttt tatcatgaat atcttactca atgtttctac gctccccaat
     1621 ttggagagcc tccttcaaac ttcattggat tgataattag tcctaaaact taggtgtttt
     1681 caagaagcat taatctttaa cttgataatt ccctgtagct tgccacaggg tttccacaat
     1741 gctgtattgt cattgcgaac gaagtgaagc aatctctcat tctagattgc ttcgtcccct
     1801 tcgacttcgc tcagggttcc tcgcaatgac agttgcagcg tttaatatta agaccatacc
     1861 ctgcagcttg ctcaatggtt attgattgat taattataaa gattctggaa ttatttttca
     1921 attctagcta agggttgatt gggtttaaac aaacttttat ctaagtcacc gtttatttcg
     1981 acttcctcat cgtactttac cgaaatggaa aatctatcaa gttttaattc tatttttgtg
     2041 gggaaagaga ccccggaacc gatgtcttta aaatccttga attcgcagtt agtttgcaca
     2101 ccgccttgaa gatttatctt tgccttttca attctataat ttatagggtt tatccagaga
     2161 acactatctt ctttgccgct gttgagaaag gttagaatga tgtagtttga ttttacactt
     2221 aattgaacct ccgaaccttc attcatctgt ctctctggaa gacgccctaa aagaagattg
     2281 accagattat ttattgtgat ttttacggga agatctggat aaatgtatga aaaatcgaat
     2341 tccttataat tatcatacac cctcttttcc tctgggagaa taacataggt ttttttacca
     2401 tcggagataa ccatgcctgc tgttctcccg aaaggagcaa ttgcttcgag atgtagaaga
     2461 ttcgattctt ctacaatcgt aacttgtcta aatgaaactg atctatccgg ggtttttata
     2521 gttatagacg caataccttt tatggatctt gcaccttcat taacttcttt tacctttgtt
     2581 aaaacttcag gaaggttaat acgacctaaa tctattacaa cggttttttt tgcgcaggag
     2641 aataaaagga ggactgctat gagtatagac cttctcaact taaatttctc cctgcctgcc
     2701 gttagacccg attttctgcc tgagaacgtt tatctatgtt tccagccttg tttttagttc
     2761 tttctcttct tcatctagat cttgattttt atttattagc tctaaacccc tttcataata
     2821 ttcgagtgct tttaacctgt cccctttatc attatataca tcgccgaggt gctccataat
     2881 attcggatca tctcctgtta actctgctgc tttttctaga agctcggtgg ctttatctag
     2941 gtttcccttt ttatagtaga tccatccaag gctgtctatt atgtaacctt tgtttggaga
     3001 aatttcaatt gcccttttta cgagcctttc ggcttcatct aagttttctc cactttcagc
     3061 ataagtgtaa cctataaaat ttagtgcatc cgcatggtca ggattaattt ctagtacctt
     3121 tttcatagca agaatagatt tatcttcgtt tttaataaag tagtatgaaa ccccgagcga
     3181 atagtatatc aattcactct taggatgggt atttacaaaa tcttcataaa gctttatacc
     3241 ctcttcattc ctatttaact ttctatacaa agccccgaga tagtttacaa tctgttcgtt
     3301 atcaggatcg tgttttaggg cttcctttat cattttgaga gattcgtcag tccttcccat
     3361 cttttcaaaa agatatgctc tttgaataag agcgtcgtta tacaaagcg
//
LOCUS       DATDRL010000323         4626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3020937, whole genome shotgun sequence.
ACCESSION   DATDRL010000323 DATDRL010000000
VERSION     DATDRL010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3020937"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /gene="murD"
                     /locus_tag="VI935_10765"
     CDS             complement(<1..893)
                     /gene="murD"
                     /locus_tag="VI935_10765"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HLE26112.1"
                     /translation="MELSGRKILVVGLGRTGIETVRFLLKKGAVVTASDISPIERLQQ
                     VVAELEGMGATIESGEHRLETFLWADMLVLSPGVPFGLPTVKKAINRGIEVISEIELA
                     SRFIKKPIIGITGSNGKTTTSTIIAQILEASGKSVFLGANIGTPLVQIAEEDDKYDLL
                     VLELSSFQLQGTSAFKPFIGTLLNISPNHLDHHENIGEYIGSKMKLFSNQTYNEWAIY
                     NADDNLIKGHLFKIKSKKIPFGETAIEGGVFFDGTYIRFRDELYDLTKMKLIGKHNIE
                     NAMAAIASTRILECDPRLIEET"
     gene            complement(955..2064)
                     /gene="mraY"
                     /locus_tag="VI935_10770"
     CDS             complement(955..2064)
                     /gene="mraY"
                     /locus_tag="VI935_10770"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HLE26113.1"
                     /translation="MLYLLYLLKEDFLFLNVFKYITFRSFGAGVTAFLLSIVLGRMLI
                     QILVKMRLRENIRADGPIGHQSKIGTPTMGGAFIVIAVAISTLLWVNLMVGPVWVVLL
                     FTLGYGLIGLIDDLLKLKRRKGMRAKVKLSCQVFLALVLIVILLYLVKGFTMSLRVDQ
                     ISSFPFTSVMFPFFKKAIFNLGWLYVPFAILVVVGTSNAVNLTDGLDGLAIGSMTIAA
                     ATYIVFAYLSGNLEFSRYLQIPYMPGSGELSVFLAAVGGACLGFLWYNSHPAQVFMGD
                     VGSLALGAAIGSVALIIKQEILLILVGGVFVAEAVSVIIQVTSFKLTGKRVFRMAPLH
                     HHFELHGWSESKVVIRFWIVSIVLSLIALSTLKLR"
     gene            complement(2072..3466)
                     /gene="murF"
                     /locus_tag="VI935_10775"
     CDS             complement(2072..3466)
                     /gene="murF"
                     /locus_tag="VI935_10775"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HLE26114.1"
                     /translation="MLELGRVLSSINGTLVEMKNFASPPKLEEVIFSGISTDSRKIRK
                     DELFFALHGSNFDGHDFVLEALEKGAKGAVIERPLSLIPNGKVLIKVPSTLESLGDLA
                     SAWRRSFVNLRLAAITGSNGKTTTKEMASSILSLKFSVLKNTGNFNNLIGLPLTLLKL
                     REGHNGAVVEFGMNDFGEIRRLSEIAAPDIGAITNIGRAHLEKLGGIEGVARAKAELV
                     EGFTENNTFVMNMDDPWIKKIAEPIKCHKISFGIDTPQTYISAKDIETVEFSAVRFKM
                     IVGGEELPIRIRGIGLHNVMNALCAAGIGFAFGCNTDEIQAGLERFTPAYMRLEVMET
                     PFGFRIINDTYNANPDSMRRGIEELTRLKGNGRAIAVLGDMLELGTASEVEHRSLGEF
                     LFDSKVDLVIAYGKQGEHLLEGIRGRAKCILAETHEEAAENLIKFAKEGDLVLIKGSR
                     GMQMEKVVQRLFKE"
     gene            complement(3459..>4626)
                     /locus_tag="VI935_10780"
     CDS             complement(3459..>4626)
                     /locus_tag="VI935_10780"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HLE26115.1"
                     /translation="GTNGKTTITYLLESIWKEMGKNAGVIGTVEYRYSGKKISSPMTT
                     PESLDLMRLFRDMRTSGVECIAMEVSSHALDRKRVSGCHFDAAVFTNLTQDHLDYHET
                     LENYLEAKKKLFSEVLRLSEKGEKFSIINIDDPYGKEIAKESSGKIIYYSISKRCEGV
                     HVEKASITPEGIWALFNTPWGKIEISSKLFGKHNLYNILAAATAALSLGASIEAVETG
                     ISRVESVSGRLDKVENTYGITILIDYAHTPDALRNVLMAVRPLTPKRLILIFGCGGDR
                     DPIKRPIMGRIGRELSDILIVTSDNPRTERPELIIEEIEKGVHEITSDKKNYFRIPDR
                     RDAIRKGIEIADSGDTVLIAGKGHETYQILGTKRVPFDDKDVAGNILRERIRNA"
BASE COUNT         1275 a         1033 c          859 g         1459 t
ORIGIN      
        1 gtctcctcaa tcaatctagg atcacactct aagattcttg tagaggcaat tgcagccatt
       61 gcgttttcta tgttgtgctt accaatgagc ttcatctttg ttaagtcata gagctcgtct
      121 ctaaatctta tataagttcc atcgaagaat acccctccct caatcgcggt ctctccaaat
      181 gggattttct ttgatttaat tttgaataaa tgccctttta ttaggttgtc atcagcatta
      241 tatatagccc attcgttgta ggtctgattt gaaaagagct tcattttgga ccctatgtat
      301 tcacctatgt tttcgtggtg atctaggtga ttaggagaga tattgagaag tgttccaata
      361 aagggtttaa aagcggatgt gccctgaagc tgaaagctgc tcaattccag tactaaaagg
      421 tcgtatttgt catcttcttc agcgatctga acaaggggag tgccgatatt cgcaccaaga
      481 aaaacgcttt tacccgatgc ttctagaatt tgagcaataa tagttgatgt tgttgttttg
      541 ccgtttgagc ctgtaatacc gattatcggt tttttaataa atcgtgatgc cagctcgatt
      601 tcgcttatta cttctattcc tctatttatt gcttttttga cagtggggag tccaaatgga
      661 acacctgggc ttagcaccaa catatccgcc caaaggaagg tttcgagcct gtgttcacct
      721 gattctatgg ttgcacccat cccctcgagt tccgctacaa cctgttgtag cctctcaatc
      781 ggagagatat cgcttgctgt tacaactgca ccctttttga gcagaaatct aaccgtttct
      841 atcccggttc ttcctagtcc aactacgagt attttccttc cactaagttc catttgggtc
      901 attaaactga aattgtaggg gtttgattaa tctaacccct acaatagata attcctacct
      961 gagttttagt gttgataaag caataagcga aagcactata gagactatcc agaatcggat
     1021 tacaaccttt gattcagacc aaccgtgtag ttcgaagtga tgatgaagcg gagccatacg
     1081 gaatacccgt tttccggtaa gcttgaatga agttacttgg attattaccg aaactgcttc
     1141 tgcgacaaat acaccgccca ctaaaataag aagaatctcc tgtttgatta taagtgcaac
     1201 tgagcctatt gcggcaccaa gggcaagtga accgacgtcg cccataaaaa cctgggccgg
     1261 atgagagtta taccagagaa atccaagaca tgcaccgccg acagcagcaa gaaagacaga
     1321 cagctccccg cttcctggca tgtaaggaat ttgaagatac ctagaaaact caaggtttcc
     1381 tgaaagatat gcaaacacta tgtaggttgc ggctgcaatt gtcattgaac caatagcgag
     1441 cccgtcaaga ccgtctgtaa ggtttactgc gtttgatgtg ccgacgacaa caaggattgc
     1501 aaatggtacg tataaccaac ctagattaaa aatagctttt ttaaaaaagg gaaacatgac
     1561 ggatgtaaaa ggaaaactag agatctggtc aaccctaagt gacattgtaa aaccttttac
     1621 taagtatagg agaattacaa ttaatactaa agctaaaaaa acctggcacg agagtttaac
     1681 ctttgcacgc atgccctttc ttcttttcag ttttagcagg tcatcaataa gcccaatcag
     1741 cccgtagccg agtgtaaaaa gtaggacgac ccagacaggt cctaccatta gatttaccca
     1801 aaggagcgtt gaaatagcta ctgctataac aataaaagca ccacccatag ttggtgtgcc
     1861 gattttagac tggtgaccga tgggaccatc cgcacgaatg ttttctctaa gtcgcatctt
     1921 cacaagtatt tgtataagca tccttcctaa tacaatgctt agtaaaaagg ctgtgactcc
     1981 tgctccaaac gaacgaaagg tgatgtattt aaatacattt agaaataaaa agtcttcttt
     2041 aagtaggtat aaaagataaa gcatagtatg gctactcctt aaaaagcctc tgaactacct
     2101 tttccatctg cattccacga gaccctttta tcagcacaag gtcgccttct tttgcaaact
     2161 tgattaaatt ctctgctgcc tcttcatggg tttctgccaa tatgcattta gctcttcctc
     2221 gaattccttc taaaagatgc tcaccctgtt ttccataagc tataacgaga tccactttag
     2281 agtcaaaaag aaactcacct aggcttctat gctcaacctc acttgcggtc ccaagctcta
     2341 gcatatcccc aagtaccgct attgctcttc cgtttccttt taatcttgta agctcctcta
     2401 tacctcttct catcgaatcg ggattggcat tataggtgtc atttattatc ctaaatccaa
     2461 aaggtgtttc catgacctca agtctcatat aagctggagt gaatctttca agtcccgcct
     2521 gaatctcatc tgtattgcat ccaaaagcaa agccaattcc ggctgcacat agtgcgttca
     2581 taacgttatg aagacctata cctcttattc ttataggtag ttcttctcca cctacaatca
     2641 tcttaaatct aactgcggag aactccactg tctcgatgtc ttttgcagag atatatgttt
     2701 gaggagtatc aatcccaaaa ctaatcttat ggcatttgat aggctctgca atttttttta
     2761 tccaaggatc atccatgttc attacgaatg tgttgttctc ggtgaaaccc tcaacgagtt
     2821 ctgcttttgc tcttgcaacg ccttctattc ctccaagttt ttcaaggtgc gctcttccaa
     2881 tattagtgat tgctccaata tctggagctg caatctctga aagccttctt atctcaccga
     2941 agtcgttcat gccgaattca actacagctc cattgtggcc ttctctaagt tttaagagtg
     3001 tgagaggaag accgattagg ttgttgaagt tccctgtgtt ttttagcacc gaaaacttaa
     3061 gagaaagtat gcttgatgcc atttctttag tagttgtttt tccatttgat ccagtaattg
     3121 cagcgagcct taagtttaca aaacttcttc tccatgctga ggcaaggtcg cctagtgatt
     3181 ctagggtaga aggaaccttg atcaatactt tgccatttgg aataagggaa agaggccttt
     3241 caataactgc tccttttgct cccttctcaa gtgcctccaa aacaaaatca tgtccatcaa
     3301 aattacttcc atggagggca aagaaaagct cgtcctttct tatcttgcgc gagtctgtag
     3361 agatgccaga gaaaattacc tcttctaatt taggtggaga cgcaaaattt ttcatctcta
     3421 cgagggtacc atttatcgaa cttaaaaccc gccctagttc aagcattcct tattctctcc
     3481 cgcaaaatat ttcctgctac gtccttatca tcaaagggaa ccctttttgt acctaaaatc
     3541 tgatatgttt catgtccttt tccagctatg aggactgtat caccactatc tgcaatctct
     3601 attccctttc ttattgcatc acgcctatca gggattctaa aatagttttt cttgtcggag
     3661 gttatctcgt ggaccccttt ttctatttcc tctatgatga gttcagggcg ctctgtgcga
     3721 ggattatcag atgtaacaat aagaatatct gatagctctc ttcctattct tcccattatt
     3781 ggtcttttta tagggtctct gtcgccgccg catccgaaga taaggataag ccttttaggc
     3841 gttaaaggtc ttacagccat gagaacgttt cttaatgcat caggggtgtg ggcgtaatct
     3901 ataagaatag tgattccgta tgtgttttct accttatcaa gccttccaga aactgattct
     3961 actcttgaaa ttccagtttc aacagcctca atcgaagcgc ctaaagaaag agcggcggta
     4021 gcggcagcaa gtatattgta taggttgtgt ttgccaaata gttttgaact tatttcaatc
     4081 tttccccatg gtgtattaaa aagcgcccag attccttctg gagtaataga agctttttct
     4141 acatgaaccc cttcacatct tttgctgatt gaatagtaga taattttacc tgaagactct
     4201 tttgcgattt cttttccata tggatcatca atgtttatta tcgagaattt ttcacctttt
     4261 tcacttagcc tcagaacctc ggagaatagt tttttctttg cctctaagta gttctcaagt
     4321 gtttcatgat agtcaagatg gtcctgagta aggtttgtaa acacagcggc gtcgaaatga
     4381 caccctgaaa ccctctttct atctaaggcg tgtgaggata cctccattgc tatacattcg
     4441 actccggaag ttcgcatatc tctaaataac ctcattaaat ccagagattc gggtgttgtc
     4501 ataggagatg atattttctt tcctgaatac ctatattcaa cagttccaat aacgccagcg
     4561 ttcttaccca tctccttcca gattgactct agaagatagg taatagttgt tttcccattt
     4621 gttcct
//
LOCUS       DATDRL010000324         9245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2998649, whole genome shotgun sequence.
ACCESSION   DATDRL010000324 DATDRL010000000
VERSION     DATDRL010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9245
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2998649"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VI935_10785"
     CDS             <1..221
                     /locus_tag="VI935_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26116.1"
                     /translation="ERVWESFCKVLRGEESYSVFRQMGFGLSRFIWYPLSKFISFYEP
                     RRLLANTSQETNFLRFFIKLLVPLLKRI"
     gene            283..358
                     /locus_tag="VI935_10790"
     tRNA            283..358
                     /locus_tag="VI935_10790"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Pro,seq:tgg)
     gene            477..1259
                     /locus_tag="VI935_10795"
     CDS             477..1259
                     /locus_tag="VI935_10795"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HLE26117.1"
                     /translation="MTGKIIVVNLVSLPILFAFVFVFINYKFFSHSVANPFYNGNYKV
                     WAHRGYYKKFPENSIESLSEAFNRGAHGVELDIFFEEGLQDFIVSHDIPYKLHKDELL
                     TLEKVFSFVGNRGYFWVDFKRLDSVHNIDIVVDKMEKLLRKFDLFEKVIIESPEGFLL
                     RKFSQKGIHTSYWVQLPSISVLIWIKDFGYRAMIAFSNFSALSMDYRDYANQLKRNYS
                     HLPIHLFTVNNKGEMVELINNCEVKVVLSDEDYYSLKQESCL"
     gene            1382..3121
                     /locus_tag="VI935_10800"
     CDS             1382..3121
                     /locus_tag="VI935_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HLE26118.1"
                     /translation="MKLNKPDVTKGFILLLIVVGFSIPYSSLIVEIQKDIFDIKAHWP
                     LAIIKILVFSLFAPLAFIISLSIIKRYKLLAKVYALLYVGLYFIALLIIAIYYKYFGT
                     IPNFMILDSTNLGSISESVSILGKQVYFQLLGAQEWLIIFLLIISVCLTYIYLRLPNT
                     RKINGYMKAIMLIFVILVYYGLNEVQILRHRHNMHEKLIGRNAEAVLYFGFLPVYKKL
                     ISYEISNKRRVVPFPGKINGQNKRSNKIPRFKGANIIVIQVESLDSQAIDYKVNGKLV
                     MPFLRKFKNQSVYFKNFFAQHSGGGSSDADLSTLTSLLPVPNHVGLYTADYDRIQSLV
                     EVLEERGYTSLAMSPVSFVFHDKTSSYPRIGFDFFYGSEFYTGQASGWYAKDWQFYDQ
                     SVDTIKNSPKPFFAYLVTIQSHGPFKNYSETTKAKFNFDNTDLSELEFNYLLSVHEVD
                     QALERFFNEILESGLLDNTILLIYGDHVGRALDRPGCLAECISLFIYHKNLPASVETK
                     VGSHVDIAPTIVDLLDVSEPVGWLGTSLFYEGRKTVLFNDLTAIEFSNGVLQKKVATE
                     YRHFLEYSNSIVE"
     gene            3309..3382
                     /locus_tag="VI935_10805"
     tRNA            3309..3382
                     /locus_tag="VI935_10805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3343..3345,aa:Arg,seq:tct)
     gene            3393..3467
                     /locus_tag="VI935_10810"
     tRNA            3393..3467
                     /locus_tag="VI935_10810"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3428..3430,aa:His,seq:gtg)
     gene            complement(3606..3983)
                     /locus_tag="VI935_10815"
     CDS             complement(3606..3983)
                     /locus_tag="VI935_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HLE26119.1"
                     /translation="MPLTVNVKEKIPGVFIVSLAGSLDSNTYTILDKRLDLILGGSPR
                     IITLDMEHLDYISSMGVRVVIKAKKSLIKNGGDLILMNLQPQIQKVFEIINALPSQQI
                     FSSIEELDHYLDRMQRKIKEKES"
     gene            complement(4075..5253)
                     /gene="argJ"
                     /locus_tag="VI935_10820"
     CDS             complement(4075..5253)
                     /gene="argJ"
                     /locus_tag="VI935_10820"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HLE26120.1"
                     /translation="MIKIPGYLSCGISAGIKKNGKKDLCLIFSKVPAKVAGVFTTNVV
                     KAAPVILGIERVKGGLCQAIIVNSGNANACTGKRGLEDAISTSRHVANKLGIDESLVI
                     PSSTGVIGVPLPMVKIKNAIPELVSKLSEDGFLDTAEAIMTTDKFPKYASAQMKIGEK
                     LGTISAIGKGAGMIAPQMATMLCFILTDINLDQKALKKALKNSVEYSFNRIIVDGDTS
                     TNDTVLMLSNGALGNNPIKDGDKHYRQFEKTLTKLTTEIAEMVVRDGEGATKVVKIIV
                     KGTRTKKDAEKIARIIGSSLLVKTALYGEDPNWGRVLAAAGRAGVKFDPLKVDLYFGN
                     YKVARNGIEIMDESKVSHILKQPNFPITLELKSGKSSSFVIASDITMDYLKLNAHYRT
                     "
     gene            complement(5246..5980)
                     /gene="recO"
                     /locus_tag="VI935_10825"
     CDS             complement(5246..5980)
                     /gene="recO"
                     /locus_tag="VI935_10825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HLE26121.1"
                     /translation="MKSSEAFLLHKIVYGEADYILSLFTKDFGKISGLAKHAKRSRKR
                     FGGRLEPFVHLRVRFREKPGGIKFIEDSEIIQVFSTIMQDIDLFMCGSFILENVGILI
                     PEEDPNERMFNLLVETLSTLDSKKSPSNVILKFQLSALSLSGYEPNFNACVECKNLIE
                     QEGFFSIQKGGVVCNECKSSISNGFRISNDFLLAQRDWMKWNHYFLNHELKDPLEYVK
                     LLVKFTQYRTGKEIKSSKFLEELSSD"
     gene            complement(6110..7471)
                     /gene="mgtE"
                     /locus_tag="VI935_10830"
     CDS             complement(6110..7471)
                     /gene="mgtE"
                     /locus_tag="VI935_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HLE26122.1"
                     /translation="MKTMEVAQLTEKISKCLKEGNIEGITEVLLSVHTSEIAILLQSL
                     DPEDRSKVFSSLDTITASQIILELGKEERQEILSGLEPDKIANIVNEMDSDDAADLIA
                     ELSQDKARLVLAKMAPEEVKEVETLLRYPKDTAGGIMQAELVQVTNDSTVRDTINWIR
                     LIADEVEDFYEIYVTDTTDKLLGMVSLKKLVLANPMIQVGDIMEQVPVKVTPYVDQEE
                     VANIFKKYDVVSMPVVNEEGRLLGRITADDVIDVITEEASEDIYRFAGVQEYVHPIYT
                     PILTKIRLRIPWILLTLFGELLIAFVIVHTFKPTLERIAILAAFMPAIMATGGNVGLQ
                     TTTIVVRGLGMGTINIGQLFKLIFSEIKEGLFLGVICGVVAAIIGAIISMNEPEFIKL
                     AFAIFVSMISAITATSFIGAAAPLVLFKLKFDPAAASGPFLTMFNDIFGSVVYLFIAM
                     LIF"
     gene            7760..8602
                     /locus_tag="VI935_10835"
     CDS             7760..8602
                     /locus_tag="VI935_10835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HLE26123.1"
                     /translation="MGRLKTYSSFVKVEHTLFSLPLILSGSFLAERGLPEIRVLILIM
                     IAAFGARTSALGINRIIDREIDKRNPRTKGRELPSGKISLWESYAIVGAGVLIYFISA
                     YYICDLAFYLSPIPLLIFTLYPYMKRFTSLCHFGVGLGLSLAPLGGWIAVRCSLNDLF
                     PPILLSLFTFFWVSGFDIIYATLDEEFDLQSGIFSLPSRYGRGNALFISAILHLFAFL
                     TLVLLYFTEFRSIYAIVFLIISGLLLFLEHKKSSDVDLAFFKVNAVLGFVVFFFVLSG
                     IYLS"
     gene            8604..9155
                     /locus_tag="VI935_10840"
     CDS             8604..9155
                     /locus_tag="VI935_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010916719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HLE26124.1"
                     /translation="MRTIVGITGASGVIYGVNFLRKCPDDKFLIVSKWGKRVLNEELG
                     LKVEELSPWAKKTFDDWDLAAPFSSGSNPFDSLVIIPCSVSTLGKIASGIADTLITRI
                     AAVALKERRRLVIALRETPLSSIALENALRLSREGAIIMPISPPHYIEIKGVNDLVEG
                     FVDKVLKLIGVGDGGGWRHEELE"
BASE COUNT         2656 a         1737 c         1783 g         3069 t
ORIGIN      
        1 atgaaagggt ttgggaatcg ttttgtaagg tattgagagg agaggaatct tatagcgttt
       61 ttaggcaaat gggatttgga ctatcaaggt ttatttggta tcctcttagc aaattcatct
      121 ctttttatga acctagaaga cttttggcga acacatccca agaaacaaac ttcttgcgtt
      181 tttttataaa attactcgtt cctctactaa aaagaattta gtaggacacg ttgaaatact
      241 tgtcgtttta tcaatcatag gtataatctt tttctttatg atcggggcgt agcgcaggtg
      301 gtagcgcgcg gcgcttggga cgccgaggtc ggaggttcaa atcctctcgc cccgaccatc
      361 attaagctat atctgatgga tattataagg ctatttgact taaatttggt ttactgatag
      421 ttaaatcttc atttttgtgt tcgagtttaa ttgaaataat aaaattcggt ttgaacttga
      481 caggcaaaat aatagtagtg aatctagtaa gtctgccaat attattcgca tttgtatttg
      541 tgtttattaa ctacaagttt ttcagtcata gtgttgctaa tccattttat aatgggaatt
      601 ataaggtctg ggctcacagg ggttattata aaaaattccc agaaaattca attgaaagct
      661 tatctgaggc attcaatagg ggcgcgcacg gagtagaact cgatatattt tttgaagagg
      721 gtttacagga ttttatcgtg tctcacgaca ttccgtataa attacataaa gatgaactcc
      781 tgacgcttga aaaagttttt tcttttgttg gtaatagggg ttatttttgg gttgatttta
      841 agcggttgga ctctgtgcat aatatagaca ttgttgtgga taagatggaa aagttactaa
      901 ggaaatttga cttatttgag aaagttatta ttgaatcgcc ggaagggttt ctattgagaa
      961 aattttccca aaaggggata catacaagtt attgggtaca acttccctca attagtgtgt
     1021 tgatttggat taaagacttt ggatacagag ctatgattgc ctttagtaat ttttctgctt
     1081 tatcaatgga ttacagagat tacgccaatc aattaaagag aaactattct catttgccga
     1141 ttcatttatt cacagttaat aacaaaggag agatggtaga gctaatcaac aattgcgaag
     1201 taaaagtagt gctttcagat gaagattatt attccttaaa acaagagtcg tgtttgtaaa
     1261 gatgatctat ctttcattga ggaaacaaat atctgtaatt aattttctga tatgaaactt
     1321 ttaacctcta agtaatgatt gtataatctc aacgtatttt taaagcacta ttgacattaa
     1381 aatgaaatta aacaaaccag atgtaacaaa agggtttatt cttttgttga ttgtagttgg
     1441 tttttcaatt ccctattcct cactgattgt ggaaatacag aaagatattt ttgatataaa
     1501 agcccattgg ccgttggcta ttattaagat attggttttc tccttatttg cgcctttagc
     1561 attcattatc tctttatcca ttattaaaag atacaagctt ttggcgaagg tatacgcatt
     1621 actttatgtt gggctctatt ttattgcatt attgataatt gccatatatt acaagtactt
     1681 tggcacgata ccgaatttta tgatacttga ttctacgaac ctaggtagta tatccgagag
     1741 tgtatctata ttaggcaaac aagtttattt tcaactatta ggcgctcaag agtggttaat
     1801 tatttttttg ttgattattt ctgtctgcct aacctatata tatttgaggc ttccaaacac
     1861 gaggaaaata aatggctata tgaaagctat tatgcttata tttgtaattt tagtttatta
     1921 cggactaaat gaagtccaaa ttcttaggca ccgacataat atgcacgaga aattgattgg
     1981 taggaatgcc gaggctgttt tgtattttgg ttttttacct gtgtataaga agctcatttc
     2041 atatgaaatt tctaataaac gaagggtggt gccatttcca ggaaaaataa acggacaaaa
     2101 taaaagaagc aataagatcc cgagatttaa aggcgcaaac ataatagtaa ttcaagttga
     2161 gtcattagac tctcaggcga tagattacaa ggtcaacggt aagttggtaa tgcctttttt
     2221 gcgtaaattt aaaaatcagt cggtttattt taagaatttc tttgctcaac acagcggcgg
     2281 cgggtcctcc gatgcagact tgagtacctt aactagtttg ttgcctgtac caaatcatgt
     2341 cggtttatat acagcggatt acgaccgtat tcagtcgcta gtagaggtgt tagaagaaag
     2401 aggttacacc tccttagcga tgtctccagt ttcgtttgtt tttcacgata aaacgtcatc
     2461 atatcccagg ataggttttg atttttttta tggctccgaa ttttatacag gtcaagccag
     2521 cggttggtat gcaaaagatt ggcaatttta cgatcaatca gttgacacga ttaaaaattc
     2581 cccgaaacca ttttttgcat acttagttac tatacaaagt catggcccat tcaaaaatta
     2641 ttccgagact accaaggcta aatttaactt tgacaatact gatctctccg aactggagtt
     2701 taattatctt ctcagtgttc atgaagtaga ccaggcgctg gagcgttttt ttaatgagat
     2761 ccttgaatcg ggtcttctgg ataatacgat tttactaata tatggggatc atgtcgggag
     2821 ggcgttggat agaccaggct gtcttgcaga atgcatatct ctttttatat atcataagaa
     2881 cttgcccgct agtgtagaaa ctaaagtcgg ttcgcatgtt gacatagccc ccacaattgt
     2941 tgacctatta gatgtttccg agcctgttgg ttggttagga acttctttgt tttatgaggg
     3001 tagaaaaacc gttttattta atgatttaac tgcaattgaa tttagcaatg gtgtattaca
     3061 aaaaaaagta gcaacggagt accgacactt tctagaatat tcgaattcta ttgtcgaata
     3121 atgagtaaat cacggtttta aatatgacgt aatacgtttt gcttttttac atgtccagcc
     3181 attatagcgc cttccttgca tggccagaag gggtcagtta gaattgtcct tactcaacta
     3241 ggaaataact agaaagtagc tgagatatga gcaattaagc ttgtgtaagt ctttgaaaat
     3301 atttaattgc gcccatagct cagcaggaca gagcaacggc cttctaagcc gtgggtcggg
     3361 ggttcgaatc cctctgggcg cgagcttata tggtgggtgt agctcagctt ggttagagca
     3421 ctgggttgtg gccccagagg tcgccggttc aagtccggtc acccaccctt aattttttgt
     3481 ataccggata gatatatcaa ttgcctatga agagaccgca tgattcttta tcttagactc
     3541 ataatcgccg ttcatagttc gacaagctca ctgcgaacgg cttgactcaa ataccgttca
     3601 ccaattcagc tttctttttc tttaattttt ctttgcatgc gatcaagata atgatctagt
     3661 tcttctatgc tactgaaaat ctgctgagat ggtaaggcat ttattatctc aaataccttt
     3721 tgtatttgtg gttgaagatt cataagtata agatctcccc cattttttat aagagatttc
     3781 tttgctttaa ttacaacacg cactcccata ctgctgatat aatctagatg ttccatatca
     3841 agtgtgatta tcctaggaga tccccctaaa atcaggtcta atcttttatc taaaatcgtg
     3901 taggtatttg aatcaagaga accggcaaga gaaacgataa aaaccccagg aattttttcc
     3961 ttcacattta ccgtaagcgg catatatgga tccttttttt aaatattata aactttacca
     4021 gatatttatc agccatgcta ttgctacaac tttcactagg ttttaatctt taagctaagt
     4081 tctataatga gcatttaatt ttaagtaatc catagtaata tcgcttgcaa ttacgaagga
     4141 actcgatttg ccgcttttaa gttctaatgt gatagggaaa ttaggttgtt taagaatgtg
     4201 gcttacttta ctctcatcca taatctcaat gccgtttctt gccactttat aattcccaaa
     4261 atataggtcc acttttaaag ggtcgaattt aacccccgct ctccctgctg ccgccagcac
     4321 acgcccccag tttggatctt ctccataaag tgcagttttc acaagaagag atgaaccaat
     4381 gatacgggcg attttctcag catctttttt agttctggtt ccttttacta ttattttgac
     4441 gacctttgtt gctccttccc catctcttac aaccatctct gcaatctctg tagttaattt
     4501 agtgagtgtc ttttcaaatt gtctataatg tttgtctcca tcttttatag gattgttacc
     4561 aagcgcaccg ttagaaagca taagcactgt atcattagtg gatgtatcgc cgtcaactat
     4621 gattctgtta aaagagtact ctactgagtt tttgagggct ttctttagag ctttttggtc
     4681 aaggttgata tccgtaagaa taaaacagag catcgtagcc atctgaggag caatcatacc
     4741 ggctcctttt ccgatagcag agatcgttcc taatttctcg cctatcttca tttgtgccga
     4801 tgcatactta gggaatttat ctgttgtcat aattgcctca gccgtatcca agaagccatc
     4861 ttcacttaat tttgagacaa gctcaggaat agcgttttta atctttacca ttggtagtgg
     4921 cacacctata accccagttg aggaaggtat gacaagtgat tcatctattc caagtttgtt
     4981 ggctacatgt cttgatgtag agattgcatc ttctaatcct ctttttccag tgcatgcgtt
     5041 tgcattccca ctgtttacta taattgcttg acacagtcct ccttttactc gttcgattcc
     5101 taaaattaca ggggcggcct tcacaacatt ggttgtaaaa acccctgcca cctttgcagg
     5161 aaccttagaa aatataaggc acaggtcttt tttcccgttc ttttttattc cagcggatat
     5221 tccacagcta aggtatcccg gaatcttaat cactgctaag ctcctctaaa aattttgatg
     5281 acttaatctc tttacctgta cggtattgag taaattttac aagtagtttt acatattcaa
     5341 gagggtcttt taactcatgg ttcaggaaat aatgattcca tttcatccaa tctctttggg
     5401 ccagaagaaa atcattcgat attctaaaac catttgatat actactctta cattcattac
     5461 atactactcc acccttttga atgctgaaaa atccctcctg ttctataaga tttttacatt
     5521 caacgcaggc attgaagttc ggctcgtacc cagagaggga aagggcggaa agttgaaatt
     5581 ttaatatcac gttagagggg cttttctttg agtctagagt agaaagggtt tctactagaa
     5641 gattaaacat cctttcatta gggtcttctt caggaataag tattcctaca ttctccaaaa
     5701 tgaaactgcc acacatgaag aggtctatat cttgcattat agttgaaaac acctgaatta
     5761 tctcagaatc ctcgatgaac ttaattccac ctggtttttc acgaaacctc actcgaagat
     5821 gcacaaatgg ttcgagtctt ccgccaaatc gctttctgct tctttttgca tgtttagcta
     5881 gtccacttat tttcccgaaa tcctttgtaa aaagagaaag tatataatct gcttccccat
     5941 atacgatttt atgaaggaga aatgcctcgg aacttttcat agatcttata ttattgccta
     6001 aaagtggcta tttagccaca gaggacacgg agcacacgga gagaagaaaa aacaaaaatt
     6061 ttatttttaa aatcttttct ttgtattctc tgtgctctct gtggctaact taaaaaatga
     6121 gcatagcgat aaagaggtaa actactgatc caaagatgtc gttaaacata gtaaggaaag
     6181 gaccagaggc ggcggcggga tcaaacttta atttaaataa aacaagcggc gcagccgcac
     6241 ctatgaagga ggtggcagta attgcagata tcatcgatac gaaaatggca aacgccaatt
     6301 ttataaattc tggttcattc atacttatta ttgctcctat tatagcagct acgactccac
     6361 atattacccc tagaaataaa ccttctttaa tttctgaaaa aatcaattta aataactggc
     6421 caatgttaat tgtccccata ccaaggcccc ttacaactat agttgttgtc tgaagcccaa
     6481 cgttcccgcc tgtagccatt atagcaggca taaaagcagc aaggatggca atccgttcaa
     6541 gggtgggttt aaaagtgtgc acaatcacaa atgcgatgag aagctctcca aagagggtga
     6601 ggagaatcca aggaatacgc aatcttattt tagtaagaat aggcgtatag atagggtgga
     6661 cgtattcttg tacgccagca aaacgatata tatcctccga tgcttcttct gtaataacgt
     6721 ctattacatc gtctgctgtt atgcggccta aaagccttcc ttcttcattt acaaccggca
     6781 tcgagacgac atcgtatttt ttaaaaatat tcgcaacctc ttcttggtca acatagggtg
     6841 ttactttaac tggaacctgc tccattatat cccctacttg tatcattgga ttggctaaaa
     6901 ccagtttttt aagtgatacc ataccaagaa gtttatcagt ggtatccgta acatatattt
     6961 catagaagtc ttcaacctcg tcagctatca gtcgaatcca gtttatcgta tctctcactg
     7021 tagaatcatt tgtgacctga acaagctcgg cctgcatgat tccccctgcg gtatctttcg
     7081 gataacgaag cagggtctcg acctccttaa cctcctcagg agccatcttt gcgaggacaa
     7141 gtcttgcttt atcctgggac agctctgcaa tcaggtcggc ggcgtcatct gaatccattt
     7201 catttacaat atttgcgatt ttgtccggct cgagtcctga gagaatttct tgtctttctt
     7261 ccttcccaag ttcgaggata atttgagagg cggttatagt atctaaggaa ctaaacacct
     7321 tagagcgatc ctctggatca agggattgaa ggagaatggc tatttccgaa gtatgcacag
     7381 ataatagtac ttctgtaatc ccttctatgt ttccttcctt tagacatttt gatatttttt
     7441 cggttagctg tgctacttcc atagttttca ttaattaact aatgtttaat caaactattc
     7501 taaaatgcaa actataaaag taaaggagag attcccttac tatatgagct atattatgtt
     7561 gcaaaacaat ctataactta ttgccatcaa aggaacgagc tcctcacctg tgctgagagg
     7621 ggcagagtcc cgaagcgtaa cctacgtcct cgttcgcaag caatgtcaaa aacaatcaag
     7681 ggattgcttc cctaatgctc tcaatgacaa ttatgagaga ttatatggtt aactctaaat
     7741 ggggtaaaat cctagattaa tgggaagact aaaaacatac agtagctttg ttaaggttga
     7801 gcatacactc ttttctcttc cattaatcct gagtggttca ttcctagcag aaagaggctt
     7861 gcctgagatt agagtgctta ttcttataat gattgctgca tttggggcga ggacttcggc
     7921 ccttggtatt aatagaatca ttgataggga gattgataag aggaatccga gaaccaaagg
     7981 tagggaactc ccttccggta aaataagtct ttgggaatct tatgcaattg ttggcgctgg
     8041 tgtgctgatc tactttattt cagcttatta catctgcgat ctagccttct atttatcacc
     8101 aattccgctt ttaattttta ccctttaccc atacatgaaa aggttcacct cactttgtca
     8161 ttttggagta ggacttggac tttcactagc cccacttgga ggatggatcg cagtaagatg
     8221 ttcacttaat gatttatttc cacctattct tctttctctt tttaccttct tctgggtctc
     8281 agggtttgac ataatctatg caacacttga tgaggaattt gatcttcaga gtggaatctt
     8341 ttctctacca tcaaggtatg gaaggggaaa tgcacttttc atctcggcta tcctacatct
     8401 ttttgcattt ctgacactcg ttttattgta tttcactgaa tttagatcaa tttacgcgat
     8461 agtgttttta attatatctg ggttactttt attcttggag cataaaaaat cctctgatgt
     8521 ggatttagct tttttcaagg taaacgctgt cctgggattt gttgttttct tttttgtcct
     8581 ctctgggata tacttgtcct gaaatgcgta ctattgtggg aataacaggg gcttctggag
     8641 tgatatacgg tgtcaatttt ttgcgcaaat gcccagacga taaatttcta atagtcagca
     8701 aatgggggaa aagggttctc aacgaggaat tagggctcaa ggttgaggag cttagtcctt
     8761 gggcaaaaaa gacgtttgac gattgggacc ttgcagctcc tttttcatcc ggcagcaatc
     8821 cctttgattc tctagtgata attccctgtt cagtttcaac ccttggaaag atagcctctg
     8881 gaattgccga tactcttata actcgaatcg ccgcagtggc cctcaaagaa agacgaaggc
     8941 tggtcattgc acttagagaa actccactaa gttcaattgc acttgaaaat gctcttaggc
     9001 tttcgagaga gggggcaata ataatgccta taagccctcc tcattatatc gagataaaag
     9061 gtgtaaatga tctagttgaa ggtttcgtag acaaggttct aaagctgatc ggggtaggag
     9121 acggcggtgg ctggaggcat gaggagttgg agtaattatt taatgtatgt actaaaaatt
     9181 gcaacgagta aaactcggca ccaaccctct ttaatttctt atgggtagct caccagtctt
     9241 gctgg
//
LOCUS       DATDRL010000325         2433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3708886, whole genome shotgun sequence.
ACCESSION   DATDRL010000325 DATDRL010000000
VERSION     DATDRL010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3708886"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..496)
                     /locus_tag="VI935_10845"
     CDS             complement(38..496)
                     /locus_tag="VI935_10845"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HLE26125.1"
                     /translation="MKKQPRTRYKQLNLSNNTLNSSLEIYIDGASRGNPGKSGAGILI
                     KETNGSIHRIKRYLGILTNNQAEYEALIHALKAAKGLKKTHLKIYSDSLLLVSQINGN
                     WRTRDSNILALSKKARSLIKGFKHVEVHHIPRELNREADRLANAAIDEYP"
     gene            complement(459..1187)
                     /locus_tag="VI935_10850"
     CDS             complement(459..1187)
                     /locus_tag="VI935_10850"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HLE26126.1"
                     /translation="MRNQITALEALQQLDLEIGELEDDLEKYPQEISRFNKEIENTKQ
                     SLAEGKEELNQIRKRKMENESKLFGNKDKIKKAEVKLFEIKTYREYEALQKELGDVKK
                     ANADLEEQILKEMETIEGLEAEVNEKENTLDEKEKENYKIIDIYNKKISEITKAYEIK
                     KQEKKKIVSLINPEILTLYERIRKRNGVVLVQARNELCTGCNMNIPPQLFNEVLTSKR
                     MIQCPNCHRILYCEETTEDEVQTA"
     gene            complement(1333..1405)
                     /locus_tag="VI935_10855"
     tRNA            complement(1333..1405)
                     /locus_tag="VI935_10855"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1370..1372),aa:Ile,seq:cat)
     gene            1547..1690
                     /locus_tag="VI935_10860"
     CDS             1547..1690
                     /locus_tag="VI935_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26127.1"
                     /translation="MVQLHNKFTDSQVKELIERYLKKEMERKHNQQILGISKTRFFAL
                     IEF"
     gene            1876..>2433
                     /locus_tag="VI935_10865"
     CDS             1876..>2433
                     /locus_tag="VI935_10865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HLE26128.1"
                     /translation="MSLKFILLFLIFIFWIIEYVKNRKSSSRHPTCNLGLTDLINPPL
                     VSVIIPARNERENISKCLASINNQTYKELEIVVIDDGSKDGTYRAVEGMQKNNAKLKL
                     IKNGNLPEGWIGKNYAIHLGMKEARGEWLLFLDADTELYPDAISKVLSFSLSNNINMV
                     SFSPEQVLRGFWESAVQPVIYEFLSY"
BASE COUNT          679 a          459 c          429 g          866 t
ORIGIN      
        1 aaaacccagc gatcgcccag ttcactcgtc tcagtattta aggatattca tctattgctg
       61 cgtttgcaag tctgtctgct tctctgttta attcccttgg aatgtgatga acctctacgt
      121 gtttaaaccc cttaattagg cttctagctt ttttggaaag tgctaaaata ttagaatcac
      181 gggtgcgcca gtttccgttt atctggctga ctagtaaaag tgaatctgaa tagattttta
      241 ggtgggtttt tttaagtcct tttgctgcct taagggcatg tattaaggct tcgtattcag
      301 cttgattatt tgtaagaatc ccaaggtatc tctttattct atgaatgctg ccgttcgtct
      361 cttttattaa tatacctgca cctgacttgc ctgggtttcc tctcgaggcg ccatcaatgt
      421 agatttcaag agatgagttc aaggtattgt tagacagatt aagctgtttg tacctcgtcc
      481 tcggttgttt cttcacaata gaggattctg tgacagtttg ggcactggat catcctttta
      541 ctggtaagga cttcattaaa aagctgtggg gggatattca tattacatcc tgtacatagc
      601 tcatttcttg cttgaacgag tacaacacca tttctttttc ttattctctc gtaaagcgta
      661 agtatttctg ggttaataag tgaaactatt ttcttctttt cctgcttctt aatttcatag
      721 gcctttgtta tctcactaat ttttttatta tagatgtcta ttattttgta attctccttt
      781 tctttctcat ccagtgtgtt ctccttttca ttaacctctg cctctagccc ctcaattgtc
      841 tccatttctt ttagtatttg ttcttcgagg tctgcgttgg cctttttaac atcacctaac
      901 tctttttgaa gagcctcata ctccctatat gtttttatct caaaaagctt tacctcagcc
      961 ttttttattt tatctttatt tccgaatagc tttgactcat tttccatctt cctttttctt
     1021 atttggttga gttcttcttt tccttcagct agtgattgtt tagtgttctc tatttcttta
     1081 ttgaatcgtg atatttcttg aggatatttt tctagatcat cttccaactc cccaatttct
     1141 aaatcaagct gctgaagtgc ctcaagggct gtaatctggt tcctcatttt tgcctccttg
     1201 ttttattgtt gtatttataa agtcttggaa tggttttatc acagaaggta aatacgataa
     1261 aaagacatga ctgagaagat tatttaggta agtttctttt accatctaaa aacctagaac
     1321 taccattacg cgtgggccca cctggattcg aaccagggac cctccggtta tgagccggga
     1381 gctctaccaa ctgagctatg ggcccaattc ttttatctga taaacaaata actataggtg
     1441 aataacttac ctttgtcaaa tatatagtat actcttttgt catttaaaaa ttaaaagtga
     1501 aaaccaagaa gccatagact aaaaccgaaa aaggaggttt tagtctatgg tccaattaca
     1561 taacaaattc accgatagtc aggttaaaga gcttattgaa cgctatttaa aaaaagaaat
     1621 ggaaagaaag cataatcaac agattcttgg tattagcaaa acaagatttt ttgctttaat
     1681 agaattttaa cgtaaaaatt caaataagtt cccagtgagg aagaagtcct taggtttcca
     1741 aacaaatttc cactgtagag atgatattta tggcgtctca ctcactaatg cgatcaacag
     1801 gcagcgaaca gacccgggta tgcatgacgt cctaatattg taaatctcca aatactatag
     1861 taaattcaaa ctctaatgtc cttaaaattt atattgcttt ttctgatatt tattttctgg
     1921 ataatagagt acgtaaaaaa tagaaaatcc tcatcacgtc atcctacttg taatttgggg
     1981 ttaactgact taattaaccc tcccttggtt tctgttatta tcccggctag aaacgagagg
     2041 gaaaacatat ctaaatgcct agcgtcaatt aataaccaga cttataagga attagaaatt
     2101 gtcgtcatag atgatggatc aaaagatggc acttatcgag cggtagaggg tatgcaaaaa
     2161 aataatgcga aattaaagtt aattaaaaat ggaaacctac cagagggttg gatagggaaa
     2221 aactatgcta ttcacctcgg tatgaaagag gcccgtggag aatggcttct ttttctcgat
     2281 gctgatacag agctctaccc tgatgcgata tcaaaggtgc taagctttag tttatcaaac
     2341 aacataaata tggtttcttt tagccctgag caggtgcttc gtggattctg ggaatctgct
     2401 gtacagcctg ttatttatga gttcttgagc tat
//
LOCUS       DATDRL010000326         5390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2198781, whole genome shotgun sequence.
ACCESSION   DATDRL010000326 DATDRL010000000
VERSION     DATDRL010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198781"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..744
                     /locus_tag="VI935_10870"
     CDS             31..744
                     /locus_tag="VI935_10870"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HLE26129.1"
                     /translation="MPRLNVNIDHVATVRQARGGIEPDPVFAAYLAELAGANGIVVHL
                     REDRRHIQDRDLRILRQTVKTKLNLEMAPTREMKEIALEVKPDMVTLVPEKRQEITTE
                     GGLDVLANSKQLLRVVGDLKEKGLFVSLFIDPDIKQIKSAKVTKADMLEIHTGGYTEA
                     KIYDKREKEFKKIEEAVKTTLEQGLKVAAGHGLNYINVKSISSIVGIEELNIGHSIIA
                     RSVLVGVERAVREMLELCK"
     gene            814..1191
                     /gene="acpS"
                     /locus_tag="VI935_10875"
     CDS             814..1191
                     /gene="acpS"
                     /locus_tag="VI935_10875"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HLE26130.1"
                     /translation="MILGIGVDLVNIPRIERIIKKWGDKFKKRAFSESEIEYSEGYNR
                     SYEHFAANFAVKEAFFKALGKGFRGNMRIHDVEVLRDENGKPYVNLHGKAKRSTELLG
                     VNSIHTSISHDVEYSVAVVILER"
     gene            complement(1385..1855)
                     /locus_tag="VI935_10880"
     CDS             complement(1385..1855)
                     /locus_tag="VI935_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HLE26131.1"
                     /translation="MLISKATPKSERKKALDEYILKKRLKSTKQREIIFDEFFNYPGQ
                     HVTVEELYEKLKKQNPKIGYATVYRTLKLFKECRLAFERNFGDGRARYEPVKFEGEHH
                     DHLICIGCGRIIEFANLRIEQYSREVAKLNDFEVLNHKLELYGYCSSCTRKRDK"
     gene            2460..2804
                     /locus_tag="VI935_10885"
     CDS             2460..2804
                     /locus_tag="VI935_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HLE26132.1"
                     /translation="MKDCIFCKIAEKKAPASIIYEDDLSIAFKDINPQAPTHILIIPK
                     KHLRTVVEATEGDKELLGHLIYVANNVANNLNVSNRGFRLVINCGAQSGQMVWHVHVH
                     LLGGRHMTWPPG"
     gene            2839..4086
                     /locus_tag="VI935_10890"
     CDS             2839..4086
                     /locus_tag="VI935_10890"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HLE26133.1"
                     /translation="MNDAIAYLNSLGLHKVKPGLERTVRLLEILGNPQEMVPSILIAG
                     TNGKGSVASTIASVLETQGYITGLYTSPHLIRVSERIRINSNEISMSDLSKLILEVKQ
                     ASSTLPDEPSYFEVLTVSAFLYFAQRKVDFSVLEVGMGGRLDATNVVKPISTVITNIS
                     KDHTEYLGDSIREIAFEKAGIIKNRVPLVTSAKEEALIVIEAVASENLAPISIFGKDF
                     NVEGESTGDFSYIGSPWNMEHLEFGLQGFYQLENASLSIAALESFSKLYKVNINEESL
                     RKGLSSIRWEGRMEIVKRDPPLILDGAHNPGAAEALRESIKKMFPGMKFVILIGMLSD
                     KDHEGYMRAIYPIAEYMIVTDVPSERTIKAKWLADAARRYISKTKVISDFKEAYKEIL
                     SLQKPVCVTGSLYLIGAIKQFFR"
     gene            4283..5317
                     /locus_tag="VI935_10895"
     CDS             4283..5317
                     /locus_tag="VI935_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HLE26134.1"
                     /translation="MTKLGIVLDKLYFEHDNGMGHPESQERLFAIVDMLRETRLFEEV
                     VRIEPRDATKEEITLVHDPKYFDLIQSTKGKIRVFLDPDTSTCPVSFDAAVRAVGGML
                     DATDKVMKGEVDFAFPLVRPPGHHAERNRAMGFCLFNNVAVGAAYASKVYGVKRILIV
                     DWDLHHGNGTQNMFYDSSEVLYFSTHQYPYYPGTGSVNEVGVQNGFGYTINVPIHPGM
                     GDKEYIKIFFEILKPVIEQYKPELILVSAGFDTYFGDPLGGMSVTSNGFAQMTRFLKE
                     MAETYCGAKLIFILEGGYNLEGLWESTKAVIEELLEKNKTDYGELTAETKVDPIIERV
                     KKVQSVFWTF"
BASE COUNT         1770 a          939 c         1108 g         1573 t
ORIGIN      
        1 acagtgtgta ctcaattgaa ataagtagaa atgccgagat tgaatgtaaa catagaccat
       61 gtagctacag ttaggcaggc aagaggaggg atagaaccag accctgtttt tgctgcctat
      121 ttggcagagc ttgctggggc aaatgggata gttgttcatc ttagagaaga cagaaggcat
      181 attcaagata gggatctaag aatccttaga caaactgtga aaacaaaact caacttagaa
      241 atggcaccta cccgagagat gaaggagata gcattagaag taaagccaga tatggtaacg
      301 ctagtgcctg aaaaaagaca agaaataaca accgaagggg gactagatgt cttagctaat
      361 tccaagcagt tattgagagt tgtaggagac cttaaggaaa aaggcctttt tgtaagtctt
      421 tttatagatc ctgatataaa gcagattaaa tctgcaaagg ttactaaagc cgacatgctt
      481 gagattcata caggtggata tacagaggct aagatttatg ataagaggga aaaggaattt
      541 aaaaaaatcg aagaggctgt taagactaca cttgaacaag gattgaaggt tgctgctgga
      601 catggcttaa actacataaa tgtgaaaagt atttccagca tagtaggaat agaggaactc
      661 aatataggcc atagcatcat agctcgctca gttttagtag gtgttgaaag agctgtaagg
      721 gagatgcttg agctatgtaa gtgagggtac gtggttcgtg atttgctgtt cgataataca
      781 caatacccaa gtcatgatac ccgaatcaca aatatgatct taggtatagg tgttgacctt
      841 gtaaatatcc ctagaataga aagaattata aaaaaatggg gagacaagtt taaaaagcgg
      901 gctttctccg aaagcgaaat agaatattct gaaggttata atagaagcta cgagcatttt
      961 gcggcaaact tcgcggtaaa agaggccttt tttaaagccc taggaaaagg tttcagaggt
     1021 aatatgcgaa ttcatgatgt cgaagtccta cgagatgaaa atggaaaacc ttatgttaat
     1081 cttcacggaa aagcgaagcg atcaacagag ctattgggcg taaactcaat tcatacttca
     1141 atatcacatg atgttgaata ctctgtagct gttgtaatac ttgaaagata gattcgagtt
     1201 atattttcaa ttactcaggg ctataacgcc tgtataattg agttagattt atgtagttga
     1261 tatagaagag taattctcat gaagaaggtc tcgatttgaa tcaaataggg ctaaagggta
     1321 tgtactcata taaaaatcag gctcgagtat gcttcattta ttacacgata aacactgctt
     1381 agatctattt gtcccttttt cgtgtacatg atgagcagta accataaagc tcaagtttat
     1441 ggttcaatac ctcaaaatcg tttaacttag caacctctct tgaatattgc tcaattctta
     1501 aattggcaaa ctctattatc cttccacatc caatgcaaat aaggtgatca tggtgttcgc
     1561 cttcaaactt aacaggctca taacgagccc tcccatctcc aaaatttctt tcgaaagcta
     1621 gtctacactc tttaaacaac tttagcgttc tataaactgt tgcgtagcct atctttgggt
     1681 tctgcttttt aagcttttcg taaagctctt ctaccgtcac gtgctgaccg ggataattaa
     1741 aaaactcgtc aaagataatt tccctttgct tcgtggattt gaggcgtttt tttaatatat
     1801 actcatctaa tgcctttttt ctctctgact ttggtgttgc cttcgaaatc aacatttcaa
     1861 ttcccccttt acaaattgaa attcaatatc aatattacaa taactatgat cttaaatcaa
     1921 gaggatacca tatagtaata attaccaata acctatcaaa tttaaaatca cctttatatt
     1981 attgactcat gtttaaatac tagaatcatc ttaattccca gctattaatc aataactctg
     2041 cagcaaggag gctgtccgat aaatgcaaaa atcggcattc ttgggggtag caaaacccct
     2101 cccagattgc cgatcgttga tcctgggtca ttctgagggg tcaaattttt accatccata
     2161 attgtcggac agcctgtgca agctgcagga tatggtcatg atattaaacg ccccaactgt
     2221 cattgcgagg agcccttctc ctggctcagg ataaactccg tgacgaagga atctaaaatg
     2281 agagatcgct tcacggagcc tgcactgaac gaagtgagcg tgttcgaaat gacagattaa
     2341 acactgtagc aacaagctac agggaatcat caagttaaat aactaatatg taagtaaggc
     2401 tacttttttt aatcatctag cagtattaag ttttatgata taatacaaaa ggatcataga
     2461 tgaaggattg tattttctgt aaaatcgctg agaaaaaagc acctgcttca ataatttatg
     2521 aagatgatct gtcaattgcg tttaaggata taaatcctca agcaccaaca cacatcttga
     2581 taattccaaa gaaacaccta cgtacagttg ttgaagcaac tgaaggagat aaggagcttc
     2641 tcgggcatct catctatgtt gcgaataacg tagcaaacaa tctcaacgtt agtaacaggg
     2701 gatttaggct cgttataaac tgtggggcgc aaagtggaca gatggtctgg catgttcatg
     2761 ttcacctttt gggtgggagg cacatgactt ggcctccagg ttgatgatgc ttctcaaagc
     2821 tttttaacaa ctccaataat gaacgacgca attgcctatc ttaattctct cgggcttcat
     2881 aaggtaaagc ctggtcttga aagaactgta aggcttcttg agattcttgg taatcctcaa
     2941 gaaatggtac caagcatact aatagcagga acaaatggca aaggttctgt ggcttcaacc
     3001 atagcttctg tgctagaaac ccaaggctat ataaccgggc tttatacatc tcctcacctt
     3061 atacgagttt cagagagaat acgaataaac agtaatgaaa tctctatgag tgatttatct
     3121 aaactcatct tagaggttaa acaagcttcc tctacgctac cagatgagcc aagctatttt
     3181 gaggtgctaa ctgtttctgc atttttatat tttgctcaga gaaaagtaga tttctcagtg
     3241 cttgaagtag gaatgggagg gcgtcttgat gcaactaatg tggtaaaacc aatttcaacg
     3301 gtgataacaa acatttcaaa agaccatacc gaatatctag gtgatagcat tagagaaata
     3361 gcctttgaaa aagcaggaat aataaaaaat agagttcctc ttgtaactag tgcaaaagaa
     3421 gaggcactaa ttgtaataga ggccgtagcc tctgaaaatt tagctccaat cagtatattc
     3481 ggaaaggatt ttaatgttga aggtgagagt acaggggatt ttagttatat tggaagtcca
     3541 tggaatatgg agcaccttga gttcggactt caagggttct atcaattaga aaatgcatca
     3601 ctctccattg ccgccctcga atccttttct aagctttata aggttaatat aaatgaagaa
     3661 agcttaagaa agggactctc ctcaataaga tgggaaggaa ggatggagat tgtgaaaagg
     3721 gatccccctc ttattctaga tggtgcgcat aatcctggtg ctgccgaagc attaagagaa
     3781 tcaattaaaa aaatgtttcc aggtatgaag tttgttattt taataggcat gctttctgat
     3841 aaagaccatg agggttatat gagggcaatc tatcccattg ctgaatatat gatagtaact
     3901 gacgtgccat ctgagaggac tataaaggcg aaatggcttg ctgatgcagc taggcgatat
     3961 ataagtaaga ctaaggttat aagtgatttt aaagaggctt ataaagaaat actctccctc
     4021 caaaagccgg tatgtgttac tggctctctt tatttaattg gagctataaa acaatttttt
     4081 cgttaaaatc aattgttcaa atgctttagt tccactaata acaaaagttt agagaataca
     4141 ctcgtaatta aaaatcataa aaattaagtt ataaggcggg aaatagcata ttaatattag
     4201 aaaaagtctc attattatac ttattaagct ttgatcccta cgattacgtg tgctactatc
     4261 aattaacagg agttaaagat aagtgactaa acttggaata gttcttgata agctttattt
     4321 tgagcatgat aatggaatgg gccatccaga gtctcaggaa agattatttg ctattgttga
     4381 catgctgaga gaaacaaggc ttttcgaaga agtagtaaga attgagcccc gagatgcaac
     4441 aaaagaagaa attacacttg ttcatgaccc taaatatttt gatctaattc aatcaacaaa
     4501 aggtaaaatc agagtcttcc tagatccaga tacctcaacc tgccctgttt catttgatgc
     4561 cgcagttcgc gcagtggggg ggatgcttga tgcaaccgat aaggtgatga aaggcgaagt
     4621 tgattttgca tttcccctcg ttcgtccgcc cgggcatcat gctgagagaa atagggctat
     4681 gggattctgc ttatttaata atgtcgctgt tggcgcggct tatgcttcta aggtttatgg
     4741 ggttaaacgt attttgatcg ttgattggga tcttcatcat ggaaatggta ctcagaatat
     4801 gttttatgat tcctcagagg ttctatattt ttctacacac caatacccct actatccagg
     4861 aacaggatca gttaacgaag ttggagtaca aaatggattt ggatatacaa tcaatgttcc
     4921 tattcaccca ggtatgggag acaaagagta tattaaaatc ttttttgaga tcttgaaacc
     4981 agttatagaa cagtataaac ctgaacttat tctggtttca gctggtttcg atacctactt
     5041 tggtgatcca cttggcggaa tgagtgttac ttctaatggg tttgcacaga tgactagatt
     5101 tttaaaagaa atggcagaga catactgcgg cgccaagttg attttcatac ttgaaggcgg
     5161 atataaccta gaggggcttt gggaatcaac taaagccgtt atagaagagc tgttagaaaa
     5221 aaataaaact gattacgggg aattgactgc agaaactaag gtagacccaa ttattgaaag
     5281 agtaaaaaag gtgcaatcag tcttctggac attctagaca ctcttttgaa atgttcttct
     5341 atagtcatcc ccttcgatag gaataaactc acacatctca cgcaaccttg
//
LOCUS       DATDRL010000327         4575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_117804, whole genome shotgun sequence.
ACCESSION   DATDRL010000327 DATDRL010000000
VERSION     DATDRL010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117804"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VI935_10900"
     CDS             complement(<1..367)
                     /locus_tag="VI935_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLE26135.1"
                     /translation="MRLLFKPRDSKYLGQGTLPYGTFDDTSEWLPITKCLEKGAEMFP
                     DKPMFKVADKDGNIVETYSYRETNEWANRISDGLRQRFGVKKGDKVGMYMINCSEYVV
                     SIIAIHKAGAVQVPINKDEK"
     gene            complement(445..777)
                     /locus_tag="VI935_10905"
     CDS             complement(445..777)
                     /locus_tag="VI935_10905"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLE26136.1"
                     /translation="MPKRVINSVAEIFGALSNPIRLQIIILLREGEKDVKEIHERLGL
                     SSSNVSQHLAILRTHHIVELSKEGTHVFYKLHTKKVIDVIRKALVLFELELSEAEAIR
                     EVIDKVGV"
     gene            complement(793..1041)
                     /locus_tag="VI935_10910"
     CDS             complement(793..1041)
                     /locus_tag="VI935_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26137.1"
                     /translation="MLREKISSGSLIEEIYKKAEKAVEFGAAMPALTLVFDDHSCFVF
                     YVEKAGDFLVLRSYDTEHKIYKSLLVIGEFEGGGSREH"
     gene            complement(1259..1453)
                     /locus_tag="VI935_10915"
     CDS             complement(1259..1453)
                     /locus_tag="VI935_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26138.1"
                     /translation="MASVTIEIPDRIFEIIKQRAQDTKSTPEAVIATNIALLFGPCIY
                     DRSKWLKNMPNPDDYSVPWG"
     gene            complement(1477..2505)
                     /locus_tag="VI935_10920"
     CDS             complement(1477..2505)
                     /locus_tag="VI935_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HLE26139.1"
                     /translation="MSKLISDEIPKPHEVKPLDALEAIGLRRSIRWYEPDKPVERWKI
                     QAMLEAARIAPTAGNYSGAKVVVCYRDEDPDIWEFISDWSQITTQMAPVLMFWCYDMA
                     GYDAQGQQLHDLMRTGALDKAHGWEYDRVSRLFPLPALLPDFVLHRLAAIDLGKAIEN
                     ALITAVALGLGTCLNGASGGARRNVKKYFSLPDSYVFCWLLTVGYPAESREAGGARGR
                     PLFETMFFERKIGNPFKRDERSVELLKQLKLIQPPGPLPGRLEELNRLTKRFGFGDEW
                     LTDWELGPSQLNDPNMLVDRRVKQLSKEEIEKVTPKNVQSEFQLKPTVKREVLDEYRK
                     KRGIGTED"
     gene            2752..3840
                     /gene="hflK"
                     /locus_tag="VI935_10925"
     CDS             2752..3840
                     /gene="hflK"
                     /locus_tag="VI935_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HLE26140.1"
                     /translation="MVDNSGRRRSDGENGETVWEELWEKWMRWRQGGGRRKTWPFLVV
                     ILIGIWLATGIYSVAPGEQGVVRQFGKEVALTGPGLRYRLPWPIETVNVVNMGVVRRV
                     EFGFRTEPRQIVPEESLMLTGDENIVDAQAIVQYKVKEPSQYLFKVVDPDQTLRDSTE
                     VALRSVIGNTTIDDAMTVGRVKVQEGAMTFLQQLLDTYETGLLVTEVKLQVVDPPGQV
                     KDAFHEVVRAREDRERLINEARGYFEDIIPKARGEVEQMIRAAEAYRERRTIQAQGDA
                     AKFLEVFKEYEKAKEVTGKRLYIETLERVLPQAEKIVIGTQSEGGLLQFLPLREAGKK
                     GERPSSIKGGDEKSSEGENQPPSGRVPR"
     gene            3837..>4575
                     /locus_tag="VI935_10930"
     CDS             3837..>4575
                     /locus_tag="VI935_10930"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HLE26141.1"
                     /translation="MKRGTAIVVALVILAFIVLPSWLFTIDETEQVIITQFGRYVRTV
                     KEPGLHAKVPFVQTVHRFEKRILVSDAPSAEYLTLDKKRLVVDYISRWKIDDPLAFFK
                     SVRTVTSAGSRINDIVFSEMRNELASHDFHMIISEKRENIMDTVASSAREKTMDFGIK
                     LIDVRIKRADLPKEVQQSVFARMMAERERIAKRYRSEGAEESAKIRAETDKQKEIMLA
                     EAYAKSQKLRGEGDAKATSIYASAYERD"
BASE COUNT         1320 a          978 c          987 g         1290 t
ORIGIN      
        1 ctttttcatc tttgttaatt ggtacctgta cagcaccagc cttgtgaatg gctatgatag
       61 atacgacgta ttcagagcag ttgatcatat acatcccaac cttatcgccc ttttttacac
      121 caaacctttg ccttagacca tcagatatcc tattagccca ctcatttgtc tccctatagg
      181 aatatgtttc aactatgttg ccgtccttgt ctgcaacctt gaacatgggt ttatcaggga
      241 acatctctgc acccttttcc aggcatttag taatagggag ccactctgat gtgtcatcaa
      301 atgtgccgta gggtaaggtt ccctgaccaa ggtattttga atctcggggc ttaaataaaa
      361 gacgcatggt atttcttttt attatttata agttataaac ctctaatctt cgaataaata
      421 taaaaatatt tcaataaagt tgaattaaac ccccaccttg tcaataactt ctcgaatggc
      481 ttctgcttct gaaagttcta actcaaaaag tacaagggcc ttacgaataa cgtcaattac
      541 ttttttagta tgtagcttat aaaagacgtg tgttccttcc ttacttaatt ctacaatatg
      601 atgagttcgt aaaatagcta agtgttgaga gacattagaa gaactgagtc ccaaccgctc
      661 atggatttct ttgacatcct tttcaccttc ccttaagagg attataatct gcaagcgtat
      721 cggatttgaa agagcaccaa aaatttccgc aacagaatta attacacgct ttggcattga
      781 aatctcacta aattagtgtt ctctgcttcc tccaccttca aattctccta ttacaagaag
      841 cgatttatag attttgtgct ctgtatcgta tgatctaagg acgaggaaat cccctgcttt
      901 ctctacatag aagacgaagc aactgtggtc gtcaaagaca agggtcaaag caggcattgc
      961 tgcgccaaac tcaacggctt tttctgcctt cttgtatatc tcttcaatta atgaaccgct
     1021 tgatattttc tctcttaaca ttttagcaag cccctttttc aacaccctag tgttctttta
     1081 aaaccctatt ctctcttcct tacagttctc cccactagac attccgccga aaggactaaa
     1141 aggggcaacc ctggagaaga gattgaccct cgcttccaaa gagggagtca tagaaggaaa
     1201 tatccgcaga gaggtgaagt tttttgtgcc gctacaattg tcttctacat cgaatacctc
     1261 atccccaagg aaccgaatag tcgtccgggt tgggcatatt cttaagccac ttcgacctat
     1321 cataaataca gggaccaaaa agaagcgcaa tgtttgtagc tataacggcc tcgggtgttg
     1381 atttcgtatc ctgtgctctt tgttttatta tctcaaaaat acggtcagga atctctattg
     1441 ttacacttgc catattctta gcctcctttt tattttttaa tcctctgtgc caatacctct
     1501 ttttttccta tactcatcta acacctccct ctttactgtc ggtttaagtt gaaattccga
     1561 ctggacattt ttaggtgtta ctttctcgat ttcttccttg gaaagttgct ttactcttct
     1621 gtctactaac atattgggat cattaagctg agatggtcca agttcccagt cagtaagcca
     1681 ttcatctcca aaaccgaatc tcttagttaa tctgttaagt tcctcaagtc ttcccggaag
     1741 cggtccagga ggttgtatga gttttagttg tttaagtagt tcaacactcc tctcgtctct
     1801 tttaaatgga ttgcctatct tccgttcaaa gaacatagtc tcaaacagag gtctgcccct
     1861 tgctccccct gcctccctgc tttctgcagg ataacccact gtgagcagcc aacaaaaaac
     1921 ataagagtca ggaagactaa aatacttttt aacgtttctt cttgctcctc cacttgcccc
     1981 gtttaaacag gttccaagcc caagagctac cgctgttatt aacgcattct ctattgcttt
     2041 tcctaaatct atagcggcaa gtctgtgaag tacaaaatct ggaagaagtg ctggcagtgg
     2101 aaacagcctt gaaactctgt catactccca tccatgtgcc ttatcaagtg ctccggttct
     2161 cattaggtcg tgtagctgct gtccctgtgc gtcataaccg gccatatcat aacaccagaa
     2221 cataagaaca ggagccatct gcgttgttat ttgactccag tctgatatga attcccagat
     2281 atcagggtcc tcgtctctgt aacaaacaac aaccttcgct ccactgtaat tgcccgctgt
     2341 tggtgctatc ctagcggctt cgagcattgc ctgtatcttc cacctttcta caggcttatc
     2401 aggttcatac caccttattg acctccttaa ccctatagct tctaatgcgt ccaagggctt
     2461 tacttcatgc ggtttgggta tctcgtctga tatcaattta ctcatttttt atccctcctt
     2521 gacaagttag aatatataaa tatactaaca ttataatatt ataaatattt ttgtcaagta
     2581 ctaatataaa taaagctagt agctcaccac aatgaaaatg agaagttgtc attctgagcc
     2641 gaagcccttc gacttgtctc aggatgaact ccgcggaggc gatgaatgac agaccatcaa
     2701 aaccgctgtt acgaactact agttaaaaaa ggtaattaag gagaaagagt gatggtggat
     2761 aatagcggaa ggagacgttc tgatggagaa aatggcgaaa ctgtttggga ggaactctgg
     2821 gaaaaatgga tgcggtggcg acaaggagga ggaagaagaa aaacttggcc cttcttggta
     2881 gtaattctaa ttggtatctg gttggcaacc gggatataca gtgtagcccc aggggagcag
     2941 ggggttgtaa ggcagtttgg gaaagaggtt gctctcactg gacctggact tcgctaccgt
     3001 cttccctggc ccatagagac tgtcaatgtc gtcaatatgg gtgtggtaag aagggttgag
     3061 ttcgggttta gaaccgagcc gcggcagata gtgcccgaag aatcccttat gctcacaggg
     3121 gatgaaaaca ttgtcgacgc acaggctatt gttcaatata aggtaaaaga accttctcag
     3181 tacttattca aggttgttga cccagaccaa accctccggg atagcacaga ggttgcactc
     3241 cgtagtgtaa ttggcaacac tacaattgat gatgccatga cagtgggcag ggttaaagtg
     3301 caagaagggg ctatgacctt tcttcaacaa ttactagata cctatgagac tggacttctc
     3361 gttacggaag tgaagctaca ggtggtggac cctccgggac aggtaaaaga cgcatttcat
     3421 gaggtggtaa gagccagaga ggatagagag agactaatta atgaggcaag gggatatttt
     3481 gaagacataa tccctaaggc aaggggagag gtagagcaaa tgataagggc tgctgaggct
     3541 tatagggaac ggagaaccat acaggcgcag ggtgatgctg caaagtttct agaggttttc
     3601 aaggagtatg agaaagcaaa ggaggtaact gggaaaagac tgtatataga gaccctagag
     3661 cgggtgttgc ctcaggctga gaagattgtc atcggcacac aatcggaggg cggtcttctt
     3721 cagttccttc ccttaaggga agcaggtaaa aaaggagaaa gaccaagtag cataaaaggc
     3781 ggggatgaaa aaagttcgga gggggaaaat caacccccct cagggagggt acccagatga
     3841 agagaggaac tgccatagta gtagccttgg tgattttagc tttcatagta ctaccctcat
     3901 ggctttttac catcgatgaa acagagcagg taattattac tcaatttgga cgctatgtga
     3961 gaactgttaa agagccggga ctccatgcca aagtcccatt cgttcaaacc gtacatcgct
     4021 ttgaaaagag gattcttgta agtgatgccc catcggctga gtacctaact ttggataaaa
     4081 agaggttggt tgttgattat atttcccgct ggaagattga tgacccactt gctttcttca
     4141 aaagtgttag aactgtaact agtgctgggt cgagaataaa cgatatagtt ttttcagaaa
     4201 tgaggaatga acttgcatct catgactttc acatgattat ctctgagaaa agggagaaca
     4261 taatggatac agtggcatct tctgcccgtg agaaaacgat ggattttgga ataaaactta
     4321 tagatgtcag gataaaaaga gccgaccttc ccaaagaggt gcaacaaagc gtgtttgcca
     4381 gaatgatggc agaaagagaa cgaatcgcca aaagataccg ttctgaaggg gctgaggagt
     4441 cagcaaaaat ccgagcagaa accgataaac agaaggagat tatgcttgcc gaggcctacg
     4501 caaaaagcca aaaactcaga ggagaaggag atgccaaagc cacatccatt tacgcctcgg
     4561 cttatgagag ggatg
//
LOCUS       DATDRL010000328         7074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2763120, whole genome shotgun sequence.
ACCESSION   DATDRL010000328 DATDRL010000000
VERSION     DATDRL010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2763120"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VI935_10935"
     CDS             <1..778
                     /locus_tag="VI935_10935"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HLE26142.1"
                     /translation="VRDMIQNHMLQMLAYIAMESPSSFHADDIRNGKVAVLKAIHPMT
                     PEDVITNAVRGQYSGGTMDGNIVLGYREEPDVAKDSRTETFASLKLFIGNWRWAGVPF
                     YIRSGKRLPTRRTEIAIQFKHAPEVLFRDTPVERLEANQLLLHIQPDQGIELVFEAKV
                     PGPTMQLQTVRMHFEYQEAFHAARGTGYETLLYDCMNGDPTLFSRSDLVETAWRVAQP
                     LLDVWSSLPPRDFPNYPACTWGPKEAAELIERDGRHWRRL"
     gene            780..1781
                     /locus_tag="VI935_10940"
     CDS             780..1781
                     /locus_tag="VI935_10940"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HLE26143.1"
                     /translation="MILAGDIGGTKTTLAFFTIDWDRLKHNLETTYHSRDYGSLDEII
                     RKFVSMNSLHVEHACFGVAGPVKEGRCEATNLPWVVDAEILTTEVGIDRVWLINDLEA
                     TGYGIKELEPKDFIILNNGADDAKGNAAVIAAGTGLGEAGLFWDGKQYFPFACEGGHA
                     DFAPRNELEMELLSHLLKHYSHVSYERVLSGPGLFNIYSFLRETGRYGKEPELLTEKI
                     SRGDDPSSAVSEAALEGSCELCVKAMNIFVSIYGAEAGNLALKALATAGVYVGGGIAP
                     KIIKKLTDGNFMKAFISKGRMEHLMQEMPVSVILNDKTALLGAARYAALKSGRGESQ"
     gene            complement(1875..3113)
                     /locus_tag="VI935_10945"
     CDS             complement(1875..3113)
                     /locus_tag="VI935_10945"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLE26144.1"
                     /translation="MTTSIRKYTVGESNAFIETFNINPKGSGPLNGLRFAVKDIIDIE
                     GFKTSCGNPYWRDSHPVAVANAVCIEQLLYAGGQCIGKTVTDELAFSLDGENFFHGTP
                     LNPKSPERVPGGSSSGSASAVACGLVDFAIGTDTGGSARVPASNCGIFGMRPSHGFIS
                     VAGVNPFAPTFDTVGVFARSSDIIAKVASVLLACDVPSTGEVGRVHLVKEAFAIADLE
                     VVQALSASIEQLRRIFGDKVKETSLLKLNGDGQVSDLQTWLNTHSTIQWAEIWSCLGS
                     WIEYLKPEFGPRIKRNFELTKNLDRGKILEAIRRREVYFRSLKSFLRNNDLLCIPTAP
                     SVAPLKGSLGNDRTTCSYYPRALSLTSIAGIGRLPQVTLPLADVHGAPIGLSLLASHG
                     QDAFLLGVVQMVTSQVKQHI"
     gene            3636..4934
                     /gene="nuoF"
                     /locus_tag="VI935_10950"
     CDS             3636..4934
                     /gene="nuoF"
                     /locus_tag="VI935_10950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017263697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HLE26145.1"
                     /translation="MPEMRIIRTNVDRPNSESIESYIASGGYTAFPKALNMDPLEIIE
                     EVKKSGLRGRGGAGFPTGIKWSFIQRDSKKPIYLCCNADESEPGSFKDREIIERDPHQ
                     MLEGIIITCYAINSHKCYIYIRGEMPKGTRILEKAIREAYDKGFLGDHISGSGFNLDV
                     VLFRGAGAYICGEETGLLESIEGKNGEPRPRPPFPAQVGLFGCPTIVNNVETLACVPH
                     IINRGAEWFASFGTPKNTGTKLYGLSGSINRPGLYELPLGIPLRELIYEYGEGIRNGR
                     QVKAISPGGGSAAVLTADEIDIPMDFDSLVKAGSMLGTAGVTVMDDTTCMVRVAQNLA
                     HFYRDESCGQCVQCREGTWWLEKMLTRIEEGKGRMEYIDTLIDACFQMKGTTICALAD
                     GCAMPVESIVKKFRHEFEEHIRLGKCPFGNKYMGIWDGSE"
     gene            complement(5084..5323)
                     /locus_tag="VI935_10955"
     CDS             complement(5084..5323)
                     /locus_tag="VI935_10955"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HLE26146.1"
                     /translation="MVMCKDVVENIMEYIDAELDDKTLEELEKHLDECPECQAFVRTY
                     KKMLELTGKLRERTFVTPDIRQRLKELLKSKLKPN"
     gene            complement(5330..5983)
                     /locus_tag="VI935_10960"
     CDS             complement(5330..5983)
                     /locus_tag="VI935_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLE26147.1"
                     /translation="MIDTITGQRGKETSPPDLVNTTDEKLIELFVSSNDERVFEEIVN
                     RYTNKIYRLAFRITRDHHSAEEVLQEIFITLINKIDTFRGGSKFSSWLYRVTANASYM
                     HLRAEKKYESDVSLEDYVPYDENGTLMGRIKANDWSERPDKALLSKEAMEIIEKAVNE
                     LPEPYRVVFHLRDVEGFSNEEVSEVLGLSVPALKSRLHRARLFLRDKLSDYFYEWSK"
     gene            complement(6101..6574)
                     /locus_tag="VI935_10965"
     CDS             complement(6101..6574)
                     /locus_tag="VI935_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26148.1"
                     /translation="MLLQFLNFTNFTGLGGLNHISYNLYQVVPVVEGFNEYGVCLDAK
                     ATIIEVDQLGFWLTDLEASRASNDDLHNCKAHDDLEAADSFFAKKFSTIQDKSLVDFK
                     KYISGNKFGLGRSFGVSFWVWKDVGLGFVLDRGEASLQVLNQSSENFLLGPRYRF"
     gene            complement(6687..>7074)
                     /locus_tag="VI935_10970"
     CDS             complement(6687..>7074)
                     /locus_tag="VI935_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HLE26149.1"
                     /translation="AKAIRLIAYTFFILGAYVLYESIKKLYFREIPDPSLLGIIIATF
                     SLMVMPILFYMKYRIGRAIEIGSLVADSKQTLACVFLSLSLLIGLGLNYLYGLWWADP
                     IVGLIIVVFLIGEGYGALKEEKLCKI"
BASE COUNT         2112 a         1504 c         1482 g         1976 t
ORIGIN      
        1 tgtaagagat atgattcaga accacatgct tcagatgctt gcctatatag ctatggagtc
       61 gccgagttct ttccatgccg atgatattcg taatggcaag gtggctgtat taaaggccat
      121 ccacccgatg actccagagg acgtcattac aaacgctgtt cgcggacagt acagcggtgg
      181 cacaatggac ggcaatattg tacttggtta tcgcgaagag ccagatgttg ctaaagattc
      241 ccggaccgag accttcgcct ctctaaagct gtttattggc aattggcgat gggcgggtgt
      301 acctttctac attcgctctg gtaagcgact accaacccgc cgtacagaaa tagccatcca
      361 gttcaagcat gccccagaag tgctattccg tgatacacca gttgagaggc ttgaagctaa
      421 ccagttgctt cttcacattc agccagacca gggtattgaa ttagtctttg aggcaaaggt
      481 ccccggacca acaatgcagt tgcaaacggt caggatgcac tttgaatatc aggaggcgtt
      541 tcacgctgca cgtggcacag ggtacgagac cttgctatat gattgtatga acggcgatcc
      601 aacccttttc tcacgttcag acttggttga aactgcatgg agggttgctc agccattact
      661 agatgtttgg agctccctgc cgcctaggga tttcccaaac tatcccgctt gcacatgggg
      721 gccgaaagag gcagcagaat taatagagcg agacgggagg cattggagga gactttagta
      781 tgattcttgc aggagatata ggtggaacca aaactactct tgcattcttt actatagatt
      841 gggatcgact gaagcataat ttagaaacca cataccatag tcgagattat ggaagcctag
      901 atgaaattat acgtaaattc gtgtcgatga actccctgca tgtagaacat gcctgtttcg
      961 gtgtggctgg accagtaaag gaagggcgtt gtgaagccac caatctacca tgggtagttg
     1021 atgcggagat actaacaacc gaagttggca tcgatagggt ctggcttata aacgaccttg
     1081 aggctactgg ttatggaatc aaagaactag agcctaagga ttttattatt cttaacaatg
     1141 gggctgatga tgctaagggg aacgccgctg taatcgcagc aggtacagga cttggagaag
     1201 cagggttatt ttgggatggc aaacaatatt tcccttttgc atgtgaagga ggtcatgctg
     1261 actttgctcc acgtaatgaa ttagaaatgg agctccttag tcatttactg aaacactaca
     1321 gccatgtgag ttacgagcga gtgttatctg gtccgggtct attcaatatt tatagcttcc
     1381 tccgtgagac agggagatac ggcaaggaac cagaattgct tacagaaaag atttcaaggg
     1441 gagatgatcc aagctcggct gtatcagagg cggcccttga aggttcttgc gagctttgcg
     1501 taaaggccat gaatatattc gtctctatat atggagcgga ggcgggcaat cttgcgctta
     1561 aggccctagc aacggctggt gtctacgtcg gcggtggcat agcaccaaaa attatcaaaa
     1621 aattgacaga tggcaacttt atgaaggcat tcattagcaa gggtcgcatg gagcacttaa
     1681 tgcaggaaat gccagtttct gtgatcctaa atgataaaac agcattactt ggtgcagcaa
     1741 gatacgcagc acttaagtca ggaagaggtg aatctcagta aagatgaatg cctgctattc
     1801 cctccgatga aattcaaagc acacccaaaa ccatgggagg tagatcttta ggctggatta
     1861 tttcccattt aatctcatat gtgttgctta acctgtgaag tgaccatctg aactactccg
     1921 aggagaaagg catcctgtcc gtgtgatgct agcaaggaaa gtccgatagg cgctccatga
     1981 acatcggcaa ggggtaacgt tacttgtggt agacgtccga ttcctgcgat agaagtcagt
     2041 gaaagagccc tgggatagta gctacaagtg gttctgtcgt ttcccagact cccttttaga
     2101 ggcgcaacgg aaggagcagt tggtatgcat aggaggtcgt tatttcgtag aaacgacttt
     2161 aagcttctaa aataaacctc ccttcttcgt atagcttcta atatcttacc tcgatctaaa
     2221 ttctttgtaa gctcaaagtt acgcttgatt cgcggaccga attccggttt tagatattca
     2281 atccatgaac ccaaacaact ccaaatctct gcccattgta ttgtggaatg ggtattaagc
     2341 caagtttgta gatccgatac ttggccatcg ccattaagtt ttagaagcga cgtctctttt
     2401 accttatctc caaatattct ccttaattgt tcgatagacg ctgagagcgc ttgtacaacc
     2461 tctaaatcag cgattgcaaa tgcctcctta acaagatgca ccctacctac ttccccagtc
     2521 gatggaacgt cacaagcaag tagcactgag gcaaccttag cgattatatc tgaagagcga
     2581 gcaaatactc ccacagtatc aaaggtagga gcaaacggat taacccctgc cactgatata
     2641 aatccatggg agggacgcat cccaaaaata ccacagttgc tcgcaggaac ccttgcggat
     2701 ccaccggtgt cagtgccaat tgcaaaatct acaaggccgc acgcaaccgc ggatgctgat
     2761 ccactggacg atcctccagg aacacgctct ggtgattttg gatttaacgg tgtgccatgg
     2821 aaaaagttct cgccgtcaag gctgaaggca agttcatctg taacagtttt accaatacat
     2881 tgcccccctg catatagaag ttgttcaatg catacagcat ttgctacagc taccgggtgt
     2941 gaatcgcgcc aatagggatt gccgcacgat gtcttaaaac cttcgatatc tataatgtcc
     3001 tttactgcaa accgaagacc atttaaaggt cccgaaccct ttggattgat attaaaggtt
     3061 tctataaatg catttgattc ccctacggta tatttcctga ttgatgtagt catctctaat
     3121 ctccctgaat taagatatct ataactatga gatttttatt atttcgaatc tccatggttt
     3181 atacaacctt atttgcctat ttcaaagtta agtgtgtatt gaaacgatta tcattttaac
     3241 tccaatcact aaacaaatat tatgcaaaaa tgaagctccc tgcggcttgc cgcagggtgt
     3301 cagcatggaa aagatagtct ctatggtaac tgcaatgaca aaacggaagt gtcattgcga
     3361 ggaacccgtc gcttagctca ggataaactc cgtgacgaag caatctaaat acgggattgc
     3421 ttcacggagc ctgcactgaa cgaagtgaac gtgttcgcaa tgacagtaga aaccctgtag
     3481 caagctacga attctcaagt taaatatcca atcaagatta atggcttttt gaataccaca
     3541 actttaataa gaattattat ttaacacaac taggttgtca tttacctaaa aatccattta
     3601 aaataatcct caacaattat tccgggaaat cgaacatgcc tgagatgaga attattcgta
     3661 caaatgttga cagaccaaac tctgagtcaa tcgagtcata tattgcatca ggtggatata
     3721 cagcctttcc taaggcttta aacatggatc ctcttgaaat aatcgaagag gtaaagaaat
     3781 caggtcttcg cggacgtggg ggagcggggt ttccgacagg aattaaatgg agcttcattc
     3841 agagggactc taaaaaaccg atttatctct gctgcaatgc cgatgaaagc gagccgggga
     3901 gtttcaagga tagagaaatc atcgaacgtg acccacacca gatgcttgaa ggaattatta
     3961 ttacatgcta tgcaatcaac tcacataaat gttatatata cataaggggt gagatgccaa
     4021 aggggacgag gattcttgaa aaggcaattc gtgaagcata cgataaagga tttctaggtg
     4081 atcacatatc agggtctggg tttaatcttg atgttgtctt attcagaggt gctggcgcat
     4141 atatctgcgg agaagaaacc gggcttctag agtcaatcga agggaaaaac ggagagccta
     4201 gacctaggcc acccttccct gcacaggtgg gacttttcgg atgcccgaca atagtaaaca
     4261 acgtcgagac tcttgcatgt gtaccacata taattaacag aggtgccgag tggtttgcca
     4321 gctttggaac tcctaaaaat acaggcacga aactctatgg gctaagtgga agtatcaaca
     4381 gacccggact ctatgaactt cccctcggaa tccctcttcg tgagctaatt tacgaatatg
     4441 gtgagggaat aagaaatgga agacaggtaa aggcaatctc ccccggtggt ggatccgcag
     4501 cggtgctcac agcagacgaa atagatattc cgatggattt tgattcctta gtaaaagcag
     4561 gttctatgct tggcactgcc ggggtaactg tgatggatga tactacctgt atggtaaggg
     4621 tcgcacaaaa tttggctcat ttctataggg atgaatcctg cgggcagtgc gttcagtgta
     4681 gggagggaac gtggtggctt gagaagatgc ttacaaggat tgaagaagga aaaggaagga
     4741 tggagtatat tgataccctt atcgatgcct gttttcagat gaaaggtaca acaatatgcg
     4801 ctctagctga tggctgtgct atgccagttg aatccattgt aaaaaagttc cgacacgaat
     4861 ttgaagaaca cataagactt ggaaaatgcc catttgggaa taaatatatg gggatttggg
     4921 atggaagtga gtagtctcgt taatcgtgca ttgtgaatcg tgagtcgggg gaataaaaac
     4981 cagcaaccac tagtcacaaa actcgaatca cagtaagtct tgaatcttta cgcaaagcaa
     5041 gcttaataaa aaaatttgat gataaaattt taagaaggtt tcctcaatta ggtttaagct
     5101 tggattttag aagttctttg agtctttgtc taatatctgg ggtcacaaat gttctttctc
     5161 tgagcttacc tgtaagctca agcatctttt tgtatgttct tacaaacgcc tgacactcag
     5221 ggcactcatc tagatgcttc tccagttctt ctagtgtttt atcatctagc tccgcatcta
     5281 tatattccat tatgttctct actacatcct tgcacataac catttctatc tatttactcc
     5341 attcataaaa atagtctgat agtttatctc tcaaaaaaag ccttgctctg tgaagcctag
     5401 actttaatgc aggtactgaa agacctaata cctcggatac ctcctcatta gaaaaacctt
     5461 ctacatccct tagatgaaaa acaaccctgt atggttcagg tagttcattc accgctttct
     5521 ctattatctc catcgcctct ttgcttaata acgctttatc gggtctttca ctccagtcat
     5581 ttgccttaat tctacccata agcgttccgt tctcatcata cggaacgtaa tcctcaaggc
     5641 tcacatcact ctcgtacttc ttttcagccc gaaggtgcat gtaacttgca tttgctgtaa
     5701 ccctatagag ccagcttgaa aacttagacc ctcccctaaa ggtgtcgatc ttatttataa
     5761 gggttataaa tatctcttga agtacctcct ccgcactatg atggtcacga gttattctaa
     5821 atgctaagcg atatatttta tttgtgtagc gattgacaat ttcttcaaat accctttcat
     5881 catttgagga gacgaaaagc tctataagct tttcgtctgt agtgttaacc aaatcaggag
     5941 ggctggtctc ttttcctctt tgcccagtta tagtatcaat catctttctt agaaacctcg
     6001 cctctttcaa ttcctattta aaatcaagtc catttcatga tttaaaactt attttgatga
     6061 gtaatctgta aggtttttgt taacaaaaga attttcaacc ctagaaacgg tatctcggtc
     6121 ctaaaagaaa attctcagac gattgattca gaacttgaag cgatgcttct cctctatcta
     6181 aaacaaaacc caatccaaca tctttccata cccagaaact taccccaaaa ctcctaccca
     6241 acccaaactt gtttcctgaa atatattttt taaaatccac cagcgactta tcctgaatag
     6301 tggaaaattt tttagcgaag aaagaatcag cggcttctaa gtcatcgtgt gccttacaat
     6361 tatgcaaatc atcatttgaa gccctagaag cttcaaggtc agtaagccag aaacccaatt
     6421 ggtcaacctc tatgatagtt gcctttgcat caagacaaac accatattca ttaaatcctt
     6481 ctacaacagg tacaacttga tacaggttat aagaaatatg atttaaaccc ccaagcccag
     6541 taaaattagt aaaattcagg aattgaagaa gcatactaat tacaacgttt gttataatac
     6601 ttaaccttcc ttaagatcaa atccccttat ttattgtatt tgacgtctga aacatacaga
     6661 tggtttcaga attagttata acaaattcaa attttgcata gtttttcttc ctttaatgca
     6721 ccgtagcctt ctcctattag aaaaactact attattaaac ccacaatggg gtctgcccac
     6781 caaagtccat ataagtagtt aagtcccaaa cctattagca aagataagga caaaaagaca
     6841 caggctagtg tttgctttga gtctgctaca aggcttccga tttctattgc tctcccaatt
     6901 cgatatttca tataaaacaa aataggcatt accatcagag aaaaagtagc gataattata
     6961 cctaaaagac tcggatctgg aatctcgcga aagtacaatt tcttgataga ttcatataaa
     7021 acatatgctc caaggataaa aaaggtataa gctatcaatc ttatagcttt tgcc
//
LOCUS       DATDRL010000329         3741 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1176137, whole genome shotgun sequence.
ACCESSION   DATDRL010000329 DATDRL010000000
VERSION     DATDRL010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176137"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VI935_10975"
     CDS             <1..230
                     /locus_tag="VI935_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26150.1"
                     /translation="PPIIFGSKVILSNVAISIYLLYIQTKLIKFSIPIVTSVQLEALH
                     QSIFFLHYLLDKEHKANLQAAGIPDHDPVQT"
     gene            complement(290..436)
                     /locus_tag="VI935_10980"
     CDS             complement(290..436)
                     /locus_tag="VI935_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26151.1"
                     /translation="MTRAKVSIPMWLKDRVDSIITEEEARLRAKFDKVLSINSKVVGL
                     VVTR"
     gene            complement(440..1474)
                     /locus_tag="VI935_10985"
     CDS             complement(440..1474)
                     /locus_tag="VI935_10985"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HLE26152.1"
                     /translation="MKRNDKSRYNGCPMNIYGAIIPGATSLARLSVRLSKQARQRIRW
                     CDYYHRCQNISRTCRYYGISRKTFYKWKRRYLKYHLPSLENNSRRPKHSRKSKIPWEQ
                     VLLVRKLRTKYPYYSKYKLSVILKRDHSITLSASTVGRIIKKYNLFFKSPYRRKKERY
                     ASVNRKKLSKDYPIKAPGDLIESDTKHMPFLGQKRYCFVAIDCVGKAIAVKISSTSSS
                     GQNSLRIKQIKQTFPFLIKSWRNDNGGENLKDFQEALEKAEISQYFTHPSCPKDKPFV
                     ERVIGTIEREFIQQGKLACDVKTQQKLINEWLDEYHNYRPHQALGYLTPNEYYKKKIG
                     KTIKQLSPMY"
     gene            complement(2236..2520)
                     /locus_tag="VI935_10990"
     CDS             complement(2236..2520)
                     /locus_tag="VI935_10990"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HLE26153.1"
                     /translation="MKKHPKRMVKRLPITEARRHLGEVVKKIRINKDYFVLEKDGIPI
                     AGLMDIDEFEDYLELQDPKLKKQIRKSYTEYQKGKARPVEEFLRELQKAK"
     gene            complement(2775..3101)
                     /locus_tag="VI935_10995"
     CDS             complement(2775..3101)
                     /locus_tag="VI935_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HLE26154.1"
                     /translation="MKRRKKDVSQSIALTMMGLDRARKELSRLVRDAQQAHTRFLFSV
                     KGKPQAVVLGIDDYLKNVLKKERLSVIAEIQLEVKEKGLDTMTMEEIDEEIRTHRKEK
                     SKSRSK"
BASE COUNT         1046 a          758 c          710 g         1227 t
ORIGIN      
        1 tgccacccat aatttttgga tcaaaagtga ttctatcaaa tgtagccata tcaatctacc
       61 tcctctatat ccaaacaaaa ttaattaagt tcagtatacc aattgttacc tcggtacagc
      121 tagaagcatt gcatcaatca atcttttttc ttcactacct cttggataaa gagcacaaag
      181 cgaatttgca agccgcagga attccggacc acgatcctgt tcagacttaa gtaatgatct
      241 caaagcatta gccctaccac tggcttgtaa aagcatagct gcatgcactt tatcttgtca
      301 ctactaaacc aactacctta ctatttatag ataagacttt atcaaattta gctctcaagc
      361 gtgcttcctc ttctgtaata atagaatcta ctctatcttt aagccacata ggaattgaga
      421 ctttagctct tgtcattgtt cagtacatag gtgacagttg tttaattgtt ttcccaatct
      481 tctttttata atactcatta ggcgttaaat aaccaagagc ttgatgaggc ctatagttat
      541 gatactcatc aagccattca tttatgagct tctgttgagt ttttacatca caggcaagtt
      601 tcccttgttg aataaattct ctctcgattg tccctataac tcgctctaca aatggtttgt
      661 cctttggaca gctaggatga gtgaagtact gtgatatctc tgccttttct aacgcctctt
      721 ggaaatcctt taagttctca ccaccattat cattcctcca ggatttaatc aaaaatggaa
      781 atgtttgttt gatctgttta atccgtaatg agttttgccc tgatgaagag gttgaggata
      841 tcttcactgc tattgccttg cccacacaat caatggctac aaaacaataa cgcttctgcc
      901 ctagaaaagg catatgtttc gtatccgatt ctatcagatc acctggagct ttaatgggat
      961 agtctttaga gagcttctta cggttgacac tggcataacg ttctttcttc ctcctatagg
     1021 gggatttaaa gaaaagattg tattttttaa tgatcctccc gactgttgaa gctgagaggg
     1081 taatactatg atctcgtttc aaaattactg atagtttgta tttagaataa tatggatatt
     1141 tcgtcctgag ctttctaacc agtaacacct gttcccatgg aatcttagat ttacgcgagt
     1201 gttttggtct tctagagttg ttctctaaag aaggtaagtg gtatttaagg tacctccttt
     1261 tccacttata gaaggttttt cttgaaatgc cataatacct acaggtgcga gagatatttt
     1321 ggcatctatg ataataatca caccatctaa tcctttgcct cgcttgtttt gatagtctta
     1381 cagacagacg agcaaggctg gtagctcctg gtataatggc tccatagata ttcataggac
     1441 agccattata tcttgatttg tcattccttt tcatacttca taggtgttac ccatgtgtat
     1501 gaacctggac agttccaatt acagagtaaa aacaaccgtt aggattttaa actcgtttta
     1561 gagtccaatt gaaccttatt aataaatagt ggtggagatg acgggaatcg aacccgcgac
     1621 ctccgccttg cgaaagggaa agagaatggt tttcaaagct ctaataattc caaaacaatc
     1681 ctttatttac aacatgttat agcgatttcc ctttgcgagg tttggtgggg atttgtgcca
     1741 ttttgggggt gctatggata actctgttat gtcaaaaaga aaatcgcatc agtatcccat
     1801 ttcttggaaa aagatattca ctgcaaacag cgtcaaaatt gatgacccta agaatctcca
     1861 gattgggact atgttggtga ttccgtaagc aggtatagac gcttcctcag gaaaacatac
     1921 attttcatgt tatctcaccg atacacttct ctccgatgtc gaaaagaatg aagtatcaca
     1981 tcttgaccgg taatatcata gcgcagtctc cattcacctg agcgaatccg aaactgtcct
     2041 tctcctggcc tcacaccttt tagctgctta atgttgtgtg agcgggtgcg attccaaggg
     2101 tcactcctaa ggacttccac catgcgttca agatgcttga tgataggagg gtttccttgt
     2161 ttgagtttcc gagtttcccg ttcaaaggtt ggggtagtaa ggattcggta cggagaagag
     2221 gacatagatt tagtgctact tggccttttg aagctccctc aggaattctt cgacaggccg
     2281 cgctttaccc ttttgatatt cagtatagct tttgcgaatc tgtttcttca gctttgggtc
     2341 ttgaagttca agataatcct cgaactcatc aatatccatg agtccagcaa taggaatacc
     2401 gtctttttca agcacaaagt aatccttatt gattcggatt ttctttacta cctcaccaag
     2461 gtggcgtcgg gcttcagtta ttggtaagcg tttgaccatg cgttttggat gtttcttcaa
     2521 ggcgggtttc tcactgttag gtataatgaa caatattaat caacattgct cattttaata
     2581 gtaatgcata ccaaatagct gtcaagggat ggagttttct ctccaaggat tgaggatata
     2641 caacagggcg aatcgtatag ctcttattta tagatcggga aaccatatat gttcagagcc
     2701 agattttgga gtgggaacgc cttctgcgtg gagaagaccg gagacaataa cgttagtatc
     2761 gtatacagct cttatcactt agatcgggat ttggattttt cctttcgatg ggttcttatc
     2821 tcttcatcta tttcttccat agtcattgta tctaaacctt tctctttaac ttcaagctgg
     2881 atttcagcaa tcacgcttag cctctctttt ttcagcacat tttttaaata gtcatcaata
     2941 cctagcacga cagcctgagg cttacccttc acgctaaaaa ggaatctggt atgtgcttgt
     3001 tgtgcatctc gaaccagacg agagagttct tttcgtgccc ggtcaagacc catcatcgta
     3061 agtgcaatac tttgactgac atctttttta cgtctcttca ataatgttac ctcctttcag
     3121 gtagagatta agattaatgt taaccttaat tttagtataa aggatcaggg atgagtgtca
     3181 atgtattgcg tcttaatgta gtcaaactca tttcttaata ccctttcccg atttcagaat
     3241 gtcttctttt atattcacat tcctcaccat atctcagtcg ccaagctcaa tacccaatat
     3301 tgaaaatgtc caccaaacgc aaagagaatg gttttctaag ctcctaacat ccctaaacta
     3361 ccccatattt acaaagggtt atagtgatta gtcgttgtga gattttgtca ggttttacgc
     3421 cattttgtgg gtgttatgga cacctctaaa tcaagaagct cgattgttta actccataaa
     3481 atttgtggga gaagatgttc aatgttttat ttattcatga aaggtacgca caggcggttg
     3541 acaggaactc tcagactgat atgattataa cgtttgtatc aatcataaga attatatgat
     3601 tctatgacta aagtttctat acaaaggagg ggaaacagta tggcaaagcg catatcagcc
     3661 atggacttga gaaaacgcct tggacagatt atgaatgaag ttaccatacg ggatgacgag
     3721 tacatcattg aacgggatgg a
//
LOCUS       DATDRL010000330         5965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1179299, whole genome shotgun sequence.
ACCESSION   DATDRL010000330 DATDRL010000000
VERSION     DATDRL010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179299"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1098)
                     /locus_tag="VI935_11000"
     CDS             complement(163..1098)
                     /locus_tag="VI935_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26155.1"
                     /translation="MTVKGLLVLFTIFALSSNLKFSVISQGLTNKIPSFNNLVVKNQE
                     VLKRVWDSLGIVGEVPIIDFKKELVIVIVPKGRIGSAVEVSTLEQKTSAVEVRYVVIP
                     VLTDSKTHSGLDSPYLIAKLFLTDPQKLNVKFIEDLPKPPIPEGTSIGRLPVDASVLR
                     QYSNNNISQYFPLDRGNVWTYRVESQGRSYDETYSILSVSQDGWSIFDNFFGQKNIAL
                     RIDPFGIILITSDKGTRTFYNLDIQHSYKHSEFVTLAGKFDDLMVVTVPDGDKFWFKD
                     VYAKGVGLIYHEHKSPKGLVKYTLINAEVSGKEYP"
     gene            complement(1291..1821)
                     /locus_tag="VI935_11005"
     CDS             complement(1291..1821)
                     /locus_tag="VI935_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLE26156.1"
                     /translation="MARIKYIEDNQVKDPKVKEAYDRMIKKRGKVTHIYKALAYKPSI
                     ISSIGPFVAAVQEPDELDPKLKERLILKVSKINRSAYCYHAHQQISKKMGFTEHEILE
                     VDNPESANISEAEKVALRYAETMTISPGNIPDSLFNEIKRHFSDSQIVELTMLVALYN
                     MINRFNEALKLDPEEY"
     gene            complement(2031..2465)
                     /locus_tag="VI935_11010"
     CDS             complement(2031..2465)
                     /locus_tag="VI935_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26157.1"
                     /translation="MIHIQYLISTKEKEIFWIKPLWCPLARPFCISRWNQKQGINVCP
                     CYSGVRRETCGPMYVWCKYDELPNSAARTYTYNFKLSVGEDDKESSLNIGPMIPDNCP
                     RCIQYLGDICITNGEFQTPCDDFNDVISWLQTGSLFRNEEDS"
     gene            complement(2814..4940)
                     /locus_tag="VI935_11015"
     CDS             complement(2814..4940)
                     /locus_tag="VI935_11015"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HLE26158.1"
                     /translation="MEKGLFLNIALMLLLSIVSIKEANAQVFSFSIPLKEPALTSASG
                     IAVDWNGYIFVSLSTTHQVLKLGPAGDEILRIGAFGAADGEFNLPQGIAVDSEGNIYV
                     ADVLNHRIQKFDSNGNFLLKLGTNGDGDGQFSLPFGVAVDSGGSIYVADTGNDRIQKF
                     DSSGKFLLKFGANGSKKGEFDTPSGIALDSNGNIYVIDTRNNRIQKFNPGGNFKSQLG
                     SSSRFNFPSGVAFDKEGNIYVADALNNRIQKFNPGGIFKFKFGIEGTGDGQFKEPLGV
                     AVDSKGHIYVADTKNNRIQKFNPSGNLLAKFGTFGTGDGEFNSPLGVTVDSGGSVYVS
                     DSGNSRIENFDSSGNFLFRFGTFGADDGQFKAPTCVAVDSGGNIYVADTFNHRIVKFD
                     SSGELVLKFGTEGTGDGELKEPQGVAVDSDGSIYVSDTKNNRIQKFDSSGNFLLKLGS
                     DGAGDGQFDEPSGVAVDSNGNIYVADTFNNRIQKFDSSGNFLFSFGTEGTEDNQFNEP
                     RGLTVDSTGNIYVADTRNNRIKKFDSIGNFIFMFGLTGSGNGRFRLPSSVAIGNNGNI
                     YVSDVFNHRIQVFLPDTDGDGVLDPNDNCPMNHNPDQPDADNDGIGDTCEPKITCQEL
                     TATIVGTTENDTISSTSGPDVIHGLGGDDTIKGVDGDDVICGGDGNDKLFGDDGNDKL
                     DGGNGMDECAGGPCEDTTIECEIDKP"
BASE COUNT         1678 a         1343 c         1113 g         1831 t
ORIGIN      
        1 gcctcaaagt tatcacctcc ataggcattt ctacctcctt ggggatagtt tctactcaaa
       61 gcagaaatta tccctaaaac ccaggggggt gacattttca ttttgcactt aactagtgac
      121 attatcattt tgcaacaaca attattaata agaattcttg atttaaggat attccttccc
      181 gcttacctca gcattaatta aggtgtattt aaccaatcct tttggcgatt tatgttcatg
      241 ataaataagc cctacccctt ttgcataaac atccttgaac cagaatttat ctccatctgg
      301 aacagtcaca accataaggt cgtcaaattt gcccgctagg gttacgaact cagaatgttt
      361 gtaactgtgt tgtatatcta ggttataaaa agtcctagtc cctttgtcag acgtaattaa
      421 tatgatacca aaaggatcaa tccttaaagc aatgtttttt tgcccgaaga agttgtcaaa
      481 tattgaccat ccatcttgtg ataccgacag aattgagtag gtttcatcat atgaccttcc
      541 ctgagattca accctgtatg tccagacgtt tcctctatca agtggaaaat attgagatat
      601 attattgtta ctgtattgcc taagcacact tgcatcaact ggaagtcgac caattgaggt
      661 accttccgga attggaggct ttggaagatc ctcaataaat ttcacattta atttctgcgg
      721 atcggttaga aaaagcttgg caattaggta tggagaatct agcccgctgt gtgtcttcga
      781 atctgttaac acaggtataa ccacatatct aacctcaaca gcactagtct tttgttcaag
      841 cgttgagacc tctacagcgc tccctattct tcctttcggt actataacaa tcaccaattc
      901 ttttttgaaa tcaatgatag gaacctcgcc aacaatccct aatgaatccc agaccctttt
      961 tagaacctcc tggtttttaa caaccaaatt attgaatgat gggatcttat tcgtaagtcc
     1021 ctgtgagatt accgaaaact ttagattgga agataaagca aatatagtaa atagtaccag
     1081 caatcctttt acagtcactt ttttgactcc ttttatatta attatatgtc aaaaaggcca
     1141 gagatatcag atgagagctt tcaagatttt gcaaaagata acaaaaacag atcaagaatc
     1201 tagagtcatt ggggttctat taggaaggcc tcataggctt tttccctgcc ttaattagtt
     1261 cttcactaat ttgctcccaa gtgttttttt tcagtattcc tcgggatcca gcttcagagc
     1321 ctcgttaaaa cgatttatca tgttatacaa ggctaccaac atggtcagct ctacaatctg
     1381 ggagtcgcta aaatgtcttt taatttcgtt aaaaaggctg tcaggaatat ttcctgggct
     1441 aatcgtcata gtttctgcgt atcttagagc gaccttttca gcctctgaaa tattcgcact
     1501 ctcagggtta tcaacctcaa gaatttcatg ttcagtaaaa cccattttct tgcttatttg
     1561 ttgatgggcg tgatagcagt atgccgagcg gttaatcttt gagactttta gtattaatct
     1621 ctccttaagc ttaggatcaa gttcgtctgg ctcctgaaca gctgcaacaa aggggccaat
     1681 gcttgatatt atggatggct tatatgctaa tgctttatag atatgagtaa ccttacccct
     1741 tttctttatc atcctgtcat aagcctcttt cacctttggg tctttaactt ggttatcttc
     1801 aatgtattta atacgcgcca tattgttcct ccaattcatt tattccatat ttttaaaatt
     1861 tattcctttt acttaatcta agatcccgag atatataaag acccttaccg tcttcgtagg
     1921 taataaatcc taaaatgtga atcaaaagaa tttatcgctt accttttata aacgagaagg
     1981 aatgcttaag acaggccagt ttcttcaacc cttgctgaat catgaaatcc tcaactgtct
     2041 tcttcgttcc taaataaaga accggtctgt agccagctta taacatcatt aaagtcatca
     2101 catggggttt gaaactctcc attagttatg catatatctc ctagatactg gatacaccta
     2161 gggcaattat ctggaatcat tggtcctatg tttaggctag attccttgtc gtcttcaccg
     2221 acagaaagtt taaaattata tgtatatgtc ctcgcagctg aattgggcaa ctcatcgtat
     2281 ttacaccaga catacatcgg cccacaagtc tctcttctta caccactgta acagggacaa
     2341 acgtttatac cctgcttttg gttccacctg gaaatacaaa aagggcgagc tagcggacac
     2401 catagtggct ttatccaaaa gatttccttt tctttagtag atataagata ttggatatgt
     2461 atcatttgtc ctcgttttta ttacacaact tttctagtga attgtcaagt ttgtagtttc
     2521 tcataaggat ttatttaaaa atacatccat ttaaaacaat ataagcctat cctattttag
     2581 gagcaccctc taggtgccga tgaatttctc tataaaccag cgactggaaa gtcgcagcta
     2641 ttgagggttt atattcaaac aaatacaaaa cgatagccga ggttttctaa cctcatcaca
     2701 ccacgatagc cgaggttttc taacctcgga taaaggagta tggaaagaag gcacccacga
     2761 tttattcggg gaagtgccta caacgaagaa gcaagcccat cggttttgtg aggctaaggc
     2821 ttatctatct cacattctat ggtggtatcc tcacaggggc ctccagcgca ttcatccata
     2881 ccatttccac catcaagctt atcgttgccg tcatctccaa aaagcttgtc gttgccatca
     2941 ccaccgcaaa ttacgtcatc tccatcaacg ccctttatgg tatcatcccc gcctaaaccg
     3001 tggataacat ccggccctga ggtactgcta atggtgtcat tctcagttgt accaactatg
     3061 gtagcggtaa gctcctggca tgtgatctta ggctcacatg tgtctcctat accatcgtta
     3121 tcagcatcag gctgatccgg gttgtgattc atagggcagt tgtcgtttgg atcaagcact
     3181 ccgtcaccgt ctgtgtctgg aagaaaaacc tggatgcgat gattgaatac atctgaaacg
     3241 tagatgttac cattgttgcc tattgctacg cttgatggaa gacgaaacct gccatttcca
     3301 gagccggtta agccgaacat aaagatgaaa ttaccgattg aatcgaactt cttgatgcga
     3361 ttattcctcg tgtctgccac atagatgttc cccgtgctat ctaccgtcag gcctcttggt
     3421 tcattaaact ggttatcttc tgtaccttca gtaccaaaac taaaaaggaa attcccgctt
     3481 gaatcaaact tctggatgcg gttattaaag gtgtctgcga cgtagatgtt gccattacta
     3541 tccactgcta cgccggatgg ctcgtcaaat tgaccgtcgc ctgcaccatc agagcccaat
     3601 ttaagcaaga aattcccgct tgaatcaaac ttctggatgc ggttattctt tgtgtctgat
     3661 acatagatgc tgccatcgct atccactgca acgccttgtg gttccttaag ctccccatca
     3721 cccgtgccct cagtaccaaa tttaagaacg agctcaccgc tcgagtcaaa cttcactatg
     3781 cgatgattga aggtatctgc aacgtagatg ttccctccgc tatccacagc tacacatgtt
     3841 ggagctttaa actgaccatc gtctgcgcca aatgttccga acctaaatag gaaattccca
     3901 cttgaatcga aattctctat gcgactgttg ccagagtccg aaacgtaaac gctccctccg
     3961 ctgtctactg ttacgcctag gggggaatta aactctccat cgccagtccc aaaggtgccg
     4021 aacttagcta gaagattccc actcggatta aacttctgga tgcggttatt cttcgtgtct
     4081 gcaacatata tgtgcccctt gctgtccact gctacaccta gcggttcttt aaactgaccg
     4141 tcaccggtgc cctcgatgcc gaacttaaat ttgaaaatcc cacctggatt aaacttctgg
     4201 atgcggttat tgagtgcgtc ggcaacataa atgttccctt ctttatcaaa agctacgcct
     4261 gatggaaaat taaacctgct acttgagcca agctgagatt tgaaattccc acctggatta
     4321 aacttttgga tgcgattgtt ccttgtgtct ataacgtaga tgtttccatt gctatccagt
     4381 gctatgcctg aaggggtatc aaactcacct ttcttagagc cgttagcccc aaacttgagt
     4441 aggaatttcc cacttgaatc aaacttctgt attcgatcat tgcctgtgtc tgcgacatag
     4501 atgcttcctc cactatccac tgctacgccg aatggaagac taaattgacc atcgccatcg
     4561 ccattggtgc ctaacttaag taagaaattc ccatttgaat caaacttctg gatgcgatgg
     4621 ttaagcacgt ctgcgacgta gatgttccct tcactgtcca ctgctattcc ttgtggaaga
     4681 ttaaattcac catccgccgc tccaaatgcg ccgatacgca agatctcatc tccagcaggg
     4741 cctaatttga gaacctggtg ggtggtgcta agactaacaa aaatatatcc attccaatca
     4801 accgcaatgc cacttgcact cgttaaggca ggctccttta acggaatgct gaatgaaaaa
     4861 acctgtgcgt tggcttcctt tatacttaca atggataaca ggagcattag ggcgatgtta
     4921 agaaataatc ccttttccat atttctgttc tccttttagt ttacttttaa agagataacc
     4981 attttgactt tctacaatat tagggcgggc atgatggtct cctgctaaaa gcacaaggtg
     5041 taaaatacac tcttctaagc tatccttgga agctttaaaa attactagaa aactcctctt
     5101 tggcaggaag ttttgttgat gtatgaggca ataagcgatt tttggggctg acaacaaact
     5161 acccctctaa gctatccctc agttccatta cttttatcag attagcggtg aaaatcaagt
     5221 atttgtattt ctgggcaaaa ttagtacgca gcaataagtg ggatattctt gtgaatacca
     5281 cagactggca agtccggctt taaggtgttg gaaaacacct catttcgcac gccgctcttt
     5341 tcttttcgct aggcgggaca tccttgtccc gcattaacag gtagggttag aaggtcttgc
     5401 ctatcgactt tgctttgctg gggtaaaaac ccttcctcac ttatcaatgg ataggtaacg
     5461 agttcctttc ccgattaaat cgggaccaag ctcagggcaa acttgctcgt gactaaacaa
     5521 tcgccaacca gcaagcctgc cctgagcgcg gtcgaaggga ctggtgggct aacctaggtc
     5581 taggaaagct tgggtatttt tgtatccgca aagtgaaacc aaaatatcct ccataggata
     5641 agcagcgagt ttagctcgtt gcaggtgagc accctggttg agtaaggcgg ggttgaaaaa
     5701 acctaacttt ccggcatcat tattttaaag aatttgctta agttttaaca aacgatcaga
     5761 gatagcattt gttgattatc tttgaaacag gtcggttatc tttgtaacag agcaatagtg
     5821 ataaataaat taataacaat ggcgattatt tctaacaaaa gatcaaatat ccctttatca
     5881 tatttgattc attgtataaa ttgtactaaa ataaaataca gccaaaaagg atgagtaatt
     5941 ggcacaaact ttgcgaatat atttg
//
LOCUS       DATDRL010000331         2441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_930891, whole genome shotgun sequence.
ACCESSION   DATDRL010000331 DATDRL010000000
VERSION     DATDRL010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930891"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VI935_11020"
     CDS             <1..295
                     /locus_tag="VI935_11020"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HLE26159.1"
                     /translation="EMGYGKRTPVSEYRLTGRGGKGVITIKTTEKNGRVVGAFQVADD
                     MQAILITTHGKIIRMEASEISVYKRGTQGLRLIELETEETVAAVAKVIDREQM"
     gene            306..911
                     /locus_tag="VI935_11025"
     CDS             306..911
                     /locus_tag="VI935_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HLE26160.1"
                     /translation="MIKLYDHAVSGNCYKVRLALSQFGVEYERINVDIFKGEQNEPWF
                     VALNPNKKVPVLIDGDFTMWESNGILFYLGKKLAPNDLYSDDPKVFGSITQWLFFGKT
                     TIDHALARARFINRFVPKENQNEKELDALREAGKSALHILNDHLRKNNFLAGSYSIAD
                     IGCYAYVHTAGEGEVSLSPFPAVTSWCDRIRSQPGYISMED"
     gene            1310..2122
                     /locus_tag="VI935_11030"
     CDS             1310..2122
                     /locus_tag="VI935_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26161.1"
                     /translation="MKNTDFGKHLNKYLKTEDPKYLFNAIKCDYRILQGVGCDAEEEY
                     IKACDKNPDSYVKFVEIDPHTIVWETIRFWQEIIFLPGMWEKEEREKVKKYLKTIGSA
                     LIPSNPWNPGYSTEDGLTHVIPVKKGSHEMLIFQHYNKKYIHDFEKALKVFSDNIKRR
                     YSSTVKSLVDRTSDPHLRSLALAKEAFSPDEAKRNDFIRIIEEILDEHIDLDEMKKFE
                     LSDVVKATASYMAWKHRKICKTCEKKKKGQKYEYETFLRLYKRAKAYYQRYK"
     gene            2232..>2441
                     /locus_tag="VI935_11035"
     CDS             2232..>2441
                     /locus_tag="VI935_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLE26162.1"
                     /translation="MDTLPKQLSVKQASVLFNIPEWTLRGYIQRRMIPYRKVRRRVYL
                     PTDKLEAWLAQFDVEPVGKETEDGKE"
BASE COUNT          830 a          434 c          521 g          656 t
ORIGIN      
        1 agagatgggt tatggaaaaa gaaccccagt ttctgagtat agattgactg gacgtggtgg
       61 aaaaggtgtt attacaatta aaaccacgga gaaaaacggt cgtgtagtag gtgcctttca
      121 ggttgcagac gacatgcagg cgatattaat aacaactcat ggaaagataa ttcgtatgga
      181 ggcctcagaa ataagcgttt acaagagagg tacacaggga ctcaggctta ttgaacttga
      241 aactgaggaa acggttgcag ccgtggctaa ggttattgat agagagcaga tgtaacatat
      301 gaaacatgat taaactctat gaccatgcag tctcaggaaa ttgctacaag gtaaggctgg
      361 ctctaagcca atttggggta gagtatgaaa gaataaatgt ggatattttt aaaggggagc
      421 agaatgaacc ctggtttgtg gcacttaatc caaataagaa ggttcctgtt cttattgacg
      481 gagattttac gatgtgggag tcaaatggga tactttttta tctaggaaag aaactcgctc
      541 ccaacgatct ttattctgac gacccgaagg tttttggttc aataacacag tggctattct
      601 ttgggaagac gaccattgat catgcccttg caagggcgcg ttttatcaac agattcgttc
      661 ctaaggagaa ccagaatgaa aaagagcttg acgccctccg tgaagcgggc aaatcggctc
      721 ttcacatttt aaacgaccat ttaaggaaga ataatttttt agccggctct tattcgatag
      781 ctgacatcgg atgttatgca tatgtacaca ctgccggaga aggtgaggta agcctttctc
      841 cctttcccgc tgttacgagc tggtgcgatc gtatccgctc ccagccaggg tatatttcta
      901 tggaagatta gcctaatagt cacagaggac accgagcaaa atttataaaa gaatatctca
      961 atttcctctg tggcctctgt ggcaaaattt tcggatttta acctggagcg ggaaacggga
     1021 ttcgaacccg cgaccctctc cttggcaagg ggttttaata gtagttccta tttgtatgga
     1081 ttagtatggt tttcttgtaa cttcaataag ttaggttaca ttactcatca gacactaatc
     1141 cagacaaatg ggtaaaaata gacaccatat agacacctcc ttggacacct ctccaaagag
     1201 tatgaaattc aattttccga aaccctttcc tgttcgtcgt actaaaaaga aaggaaaata
     1261 caatttactt ttccctaatt cacttcgacg cacaaaatta gaaggatgtt tgaaaaatac
     1321 cgattttgga aaacatttaa ataaatatct gaagacagaa gaccctaaat atttgtttaa
     1381 cgcaataaaa tgcgattatc gaatcctcca aggtgttggt tgtgacgcag aagaagaata
     1441 tataaaagca tgtgataaaa acccagatag ttatgtcaag tttgtagaaa ttgaccctca
     1501 cacaatagtc tgggaaacaa taaggttttg gcaagaaata attttcttac caggaatgtg
     1561 ggaaaaagag gagagagaaa aagtaaaaaa gtacttaaaa actatcggaa gtgctttgat
     1621 tccatccaat ccttggaacc ctggttatag cacggaggat ggacttaccc acgtgattcc
     1681 cgtaaagaaa ggttcgcatg aaatgctaat atttcaacat tacaataaga aatatattca
     1741 tgactttgaa aaagcattaa aagtattcag cgataacatt aaaagacggt actcatctac
     1801 tgttaaatca cttgtggata gaacatctga cccacatctt agaagcctag cacttgcaaa
     1861 agaagctttc tcacccgatg aagcaaaacg taatgatttt attcggataa ttgaggaaat
     1921 acttgacgag catatagatt tagatgaaat gaaaaagttt gaactgagcg atgttgtaaa
     1981 agccacagct agttacatgg cttggaaaca caggaaaatt tgtaaaacat gtgagaaaaa
     2041 aaagaaaggt caaaaatacg aatacgaaac cttccttaga ctttataaaa gagccaaagc
     2101 atattatcaa cgctacaaat aatataaaaa atccccccaa ttgaatcaaa gttgaaattt
     2161 aacatcacct ctatcgttga ttgttatggg tgtgtcatga tattggtaaa taagccatag
     2221 gaggaaatta tatggacaca ctccccaaac agctttctgt caagcaagct tccgttctct
     2281 tcaatattcc tgaatggacg ctaagaggct atattcaaag gcgtatgatt ccgtatcgta
     2341 aggttaggcg aagagtttat cttcctactg acaaactcga agcatggttg gcacagtttg
     2401 atgtagagcc tgtagggaag gagacagaag atggcaagga g
//
LOCUS       DATDRL010000332         2013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1977617, whole genome shotgun sequence.
ACCESSION   DATDRL010000332 DATDRL010000000
VERSION     DATDRL010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977617"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..631)
                     /locus_tag="VI935_11040"
     CDS             complement(20..631)
                     /locus_tag="VI935_11040"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HLE26163.1"
                     /translation="MDSRLYKKALLLSYFTVGYNFLEGVVSILAGLLAGSIALIGFGL
                     DSFVESLSGGVMVWRLSKHGEISLEEEEKIEAKAVRLIAYTFFILGAYVLYESIKKLY
                     FREIPDPSLLGIIIATFSLIVMPILFYMKYRIGRAIEIRSLVADSKQTLACVFLSLSL
                     LIGLGLNYLYGLWWADPIVGLIIVVFLIGEGYGALKEERLCNI"
     gene            complement(699..1091)
                     /locus_tag="VI935_11045"
     CDS             complement(699..1091)
                     /locus_tag="VI935_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26164.1"
                     /translation="MLKQISLTLWGVTFLLVLGVGMLRAEERVTGEEAEKVRNVMESY
                     VKQDSTLKGGFLIYDEKGNKVLNLKYDHVHKGVEKTESDEYFACVDFVDSNKNTYDID
                     VYVGKKGEEWDITKLVIHKVNAKDRLKK"
     gene            complement(1242..1715)
                     /locus_tag="VI935_11050"
     CDS             complement(1242..1715)
                     /locus_tag="VI935_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HLE26165.1"
                     /translation="MEEKPLFIGKVAKKTSVNPKTIRYYEGINLLSKPQRERNNYRFY
                     SHDTVTRLNFIKKAQSLGFTLKEIKEILALRDRGFRPCTHVRGLFRQRVINLEQKLAE
                     LTTLRRELKKLEDEWASMKTVEDGKGEGICPQIEKAIVRLTKKPSKVLYSRNERR"
     gene            complement(1783..1956)
                     /locus_tag="VI935_11055"
     CDS             complement(1783..1956)
                     /locus_tag="VI935_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26166.1"
                     /translation="MEPKERVLLCPICGACPEVRIYDDEVRIGEEGNMVKLSVDEWNT
                     LVEKILSGALRKL"
BASE COUNT          568 a          482 c          290 g          673 t
ORIGIN      
        1 agaatcactt ataactaagt caaatattac ataatctttc ttcctttaag gctccatagc
       61 cttctcctat tagaaaaact actattatta aacccacaat tggatctgcc caccaaagtc
      121 catataaata gttaagtccc aaccctatta gcaaagataa ggacaaaaag acacaagcta
      181 gtgtttgctt tgagtctgct accaggcttc tgatttctat tgctctccca attcggtatt
      241 tcatataaaa caaaataggc attactatca gagaaaaagt agcgataatt atacctaaaa
      301 gactcggatc tggaatctcg cgaaagtaca atttcttgat agattcatat aaaacataag
      361 ctccaaggat aaaaaaggta taagctatca atcttacagc ttttgcctct attttttctt
      421 cttcctcaag tgatatttcc ccatgtttac ttaaccgcca aaccattaca ccccctgaaa
      481 gcgattcaac gaagctatca agaccaaatc caatcaaggc aatactacca gctaataaac
      541 ccgctaaaat tgatacgact ccttctaaga aattgtatcc cactgtgaaa tacgacagta
      601 aaagtgcctt tttgtagagt cgtgaatcca taatatcgat gaactagaaa ttgggtttaa
      661 atcttcgaga ttcctgagct catattaatg attaaatctc actttttcag cctatcttta
      721 gcgttaactt tgtgtataac tagcttggtt atatcccatt cttcgccctt tttaccaacg
      781 tagacatcta tatcgtaagt gtttttattg ctatctacaa aatccacaca ggcgaaatat
      841 tcatcgcttt cggttttctc gactccctta tggacgtggt catattttag attaagcacc
      901 ttatttccct tttcatcgta gattaaaaaa ccacctttca aggtgctatc ctgcttaaca
      961 taggattcca tgacattccg taccttttcc gcttcttcac ccgttactct ctcctcggcc
     1021 cgaagcattc caactccaag tacaagtaaa aaggtcactc cccacaaagt tagagatatt
     1081 tgttttagca tggttttacc tccttcttag atttattttt gtcattccac cttgctaatt
     1141 agataagtat cttcttctct acttttagaa taactgtttt gcttctgtca cggcaagcat
     1201 caactaatcc aaaatcctat ttaatcttaa aaatatccac tttatcttct ctcatttcta
     1261 gagtacaaga ccttagatgg cttttttgta agtcgaacta ttgccttctc aatttgagga
     1321 catattcctt cacctttacc atcttctact gttttcatac ttgcccattc atcttcaagc
     1381 tttttaagct cccgtctgag tgtagttagc tcagctagtt tctgctcaag attaatgacc
     1441 cgttgtctga aaagccctcg gacatgagtg caaggcctaa accctctatc ccttaaagca
     1501 agaatctctt tgatttcctt cagggtaaac cctaggcttt gggccttctt tatgaagtta
     1561 agcctcgtta ccgtgtcatg agaatagaac ctgtagttat tccgctctcg ttgaggcttg
     1621 gacaaaaggt taattccctc atagtaacgg atggttttag ggttaacact ggttttcttg
     1681 gctacttttc caataaaaag gggtttctct tccatttctt tatcctccgc ctgacctaaa
     1741 ttgaggaagg tggaggatga ccaccttccc ctacaaaacg ggttaaagct ttctaagtgc
     1801 cccactaaga atcttttcaa caagtgtatt ccattcatcc acacttagct ttaccatgtt
     1861 cccctcttct ccaattctta cctcatcatc atagatcctt acctcggggc atgccccgca
     1921 tatcgggcaa agtaacactc tttcttttgg ttccatcttt ttcacctcct cttatctggg
     1981 atctttcaga actaattata caccttccag tta
//
LOCUS       DATDRL010000333         8834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2500516, whole genome shotgun sequence.
ACCESSION   DATDRL010000333 DATDRL010000000
VERSION     DATDRL010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8834
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2500516"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1648)
                     /locus_tag="VI935_11060"
     CDS             complement(143..1648)
                     /locus_tag="VI935_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018445900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26167.1"
                     /translation="MKSNGALGDEKKMTEIYFSDYFSVSPEVVEEHGAFDISLINDLP
                     LFIDPFLLFNSEDDTYQELHAQIITYMRFLKEVSLAGTVPRPLIEEWFTFPEIKQNWF
                     GLSRVGNQGRGLGMDFADALCRNLSTVFRDFGDEVVTRSSHLEKLCLIRDGVGRDNIS
                     DFTMNLIKCYLAEFTQDFALKYLNPSQRRLVTLRKVKFNYDTRSWTTASFELPYIHDD
                     FILLTPKDILTKDESWINRPELLDRFPEIANALPDATLRAQVNDYLARVIPKGPKVKQ
                     KEIREAIGSAVERFPQVLDYYIRDKEDSGDQAVSFAEARVREVETVFIDKIRALVSQY
                     LDPGGFYRLPYNTYEEAHERVMFLKDVIENKGGHKIFYVNGIPIEREADLQIMYRLTW
                     YATQSDVTREANDGRGPADFKISRGAEDKTIIEFKLAKNTQLERNLQNQCKVYEAASD
                     TTHPSIKVILHFNDSEYERVLRILKRLNLEGNKNIVLIDACDYNKPSGSRA"
     gene            complement(1653..1979)
                     /locus_tag="VI935_11065"
     CDS             complement(1653..1979)
                     /locus_tag="VI935_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016953722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLE26168.1"
                     /translation="MSRKKPEYEMGSGNIFADLGLDEADELLIRAKLGHTVRIILEKR
                     GLKQKEISELLRIDQSEVSKLMNGKYHLFSEGRLFGFLNKLGRKVTVQISPRQKDDKP
                     QEVVFA"
     gene            complement(1960..2298)
                     /locus_tag="VI935_11070"
     CDS             complement(1960..2298)
                     /locus_tag="VI935_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLE26169.1"
                     /translation="MGDSRKRVRNFPTEVKKEIGVALFDVQMGTTPPDAKPFKGVGRG
                     VFEIVTRHATNTYRTVYAVQIGSRVYVLHAFQKKSPKGIKTAERDKDLIKSRYEQAVE
                     MEKQYEQKKT"
     gene            complement(2614..2901)
                     /locus_tag="VI935_11075"
     CDS             complement(2614..2901)
                     /locus_tag="VI935_11075"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLE26170.1"
                     /translation="MKLRWTRIALQDLRHLHEYIAEDNPLAASRMVVRIQDATQRLNK
                     HPHMGRPGRVRGTRELVIAGSPYIVVYILGDSEIQIVAVIHSAVRWPDTFR"
     gene            complement(2898..3146)
                     /locus_tag="VI935_11080"
     CDS             complement(2898..3146)
                     /locus_tag="VI935_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HLE26171.1"
                     /translation="MPKSSTRISFITRAGKRKRLGHIAEAFGKNLSAVINEALDQYIE
                     LHEWQLTHIRKGVKAARSGDFATEAEVKAFFDKYGRPS"
     gene            complement(3392..4408)
                     /locus_tag="VI935_11085"
     CDS             complement(3392..4408)
                     /locus_tag="VI935_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLE26172.1"
                     /translation="MNDEKTIPEMMTNRIKRYGGRLFLQHKEGWSWKQITWLDFEKTT
                     KDIACFLLGLGVAPGTAAIIVSGSRTESLFTEFGVYLLGGITIPIDDNEDLEKIIRIA
                     KDSKSRFMFVAKESTLKELSNKIHEVPSLHKIITFTDTGIGEDEKVIPFKGLLKFGSM
                     KRKVLEDELVRRAEGVLPGSTAAIFYNLDSDLKKERKEITHEDLIKALKAGSEKLLFI
                     TEEDQAFSHLPHVSPYERFVNYLGIYMGNRISIAENRDSFFEDILEVKPTVIYETHNG
                     IENICSKALSNPGKGTISHRLKNALGGRAKHILIDSLPRREIINLLSSSGVFLTEVPE
                     LAEL"
     gene            4476..5831
                     /locus_tag="VI935_11090"
     CDS             4476..5831
                     /locus_tag="VI935_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HLE26173.1"
                     /translation="MDKITKLEPFRGEQFNSAFNIVERLRSSGYKAFFVGGCVRDTVM
                     GIPPKEYDIATSASPEEIMRLFSNTVPIGASFGVILVLQDECKFELATFRQDERYTDG
                     RHPDRVIYTSSEKEDVTRRDFTINGMLYDPITEEVIDYVGGIEDIKNGIIRTIGNSYE
                     RFNEDKLRMIRAIRFGARFNYEIEEETLHAIRKLAYRISDVSTERVREEIVKIITQKN
                     PGVGLLLLRETGLLSHMLPEVDKMFGIPQPPEFHPEGDVFTHTCLILDKVFEITEGNT
                     TLELSVGGLLHDVGKPVTFSIEDRIRFNAHDRIGAEMAEKICKRLRFSNKEIERIKAL
                     VSEHLRFKDVFNMKESTLKRFLGMPYFEDHLILHLADCLASHGSIEAYDFVKKKLEGF
                     KEEEIKPKPILTGYDLIEMGYTPGPIFSEILESLEEVQLEGTVKDKEEAKKFVLERFP
                     I"
     gene            complement(6051..6392)
                     /locus_tag="VI935_11095"
     CDS             complement(6051..6392)
                     /locus_tag="VI935_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26174.1"
                     /translation="MEKNQQLLKETASSYYNLFMWKSYERASLFIDEEKKDKFESFLL
                     NSKDDLNITNYEIREVTFNSVENKGLVRVLISYYKYPSVSEKTILLQDPWILRGKSWY
                     IYSDFDDEIFK"
     gene            complement(6593..6928)
                     /locus_tag="VI935_11100"
     CDS             complement(6593..6928)
                     /locus_tag="VI935_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HLE26175.1"
                     /translation="MRRVLFTLCTIGFALLGAFVPEVLAQEEAPVKELGDLVKFGLAI
                     GSGLGIGIAAGIAGLGQGRATAAALSGIARNPGAAAKIQTPMIIGLALIESLVIYALA
                     ITFLLQAKI"
     gene            complement(7044..7733)
                     /gene="atpB"
                     /locus_tag="VI935_11105"
     CDS             complement(7044..7733)
                     /gene="atpB"
                     /locus_tag="VI935_11105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HLE26176.1"
                     /translation="MEHFSWFTLFGLGKYDPILGAILVVLFLIFAGYKIKKSLSNENA
                     VLPEEKFSLRTIFEILTIDFLLDLFTSMMGSREKAKTYFPLLGTSFMFILFSNLLGLI
                     PGFLSPTGNFNTPVACALVIFIMYNFYGLKENGIGYLKHFAGPIIYIAPLMLVIEIIS
                     HLVRPLSLSLRLFWNMTGDHLVLGIFTNLTHFVVPALFIALGIFVSFLQAFIFTVLSA
                     IYISLSTAHEH"
     gene            complement(7720..8106)
                     /locus_tag="VI935_11110"
     CDS             complement(7720..8106)
                     /locus_tag="VI935_11110"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HLE26177.1"
                     /translation="MTTEIMKTTSPITFSRVEKHGLIVTGILVAINAILGLREIALGV
                     GVGGLLVIVNFLAIRLVVGALIGGAHSKGFSVFVLLIKMAIFIGLLISLFTFTKINIY
                     GFLIGMVVVVIVIIGEGLRGKKDGAL"
     gene            complement(8063..8326)
                     /locus_tag="VI935_11115"
     CDS             complement(8063..8326)
                     /locus_tag="VI935_11115"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HLE26178.1"
                     /translation="MWRQVKVKEEKNLGWLRYFAIGTELAFSVIAGLIAGHYVDKWIG
                     TKTPWFTFIGLIAGVVAGFSLLIRMTKRKNDYRNNEDNKPNNV"
     gene            complement(8435..>8834)
                     /locus_tag="VI935_11120"
     CDS             complement(8435..>8834)
                     /locus_tag="VI935_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLE26179.1"
                     /translation="LSGNPVAMAAGVATINKLREKGTYKRLNELTQSLIEGLHKILKK
                     LGIKAQINSVGSMFTLFFTDRQVIDYESALNSDTKMYSRFFKNLLSAGIMFPPSQFEA
                     VFLSLAHTEREINITLRACYKALKETASGG"
BASE COUNT         2646 a         1862 c         1670 g         2656 t
ORIGIN      
        1 accatatttg tcaagaataa aaaaacccaa tgggaacccc ctgtctcaaa taattataac
       61 cattacggct ggattaaaga aggtatttcc tatctgcttt aaggaatccg tgatttcttg
      121 ctctaaagag ctgcacagta tactacgcac gtgatccgga cggcttgttg taatcacaag
      181 catcaataag cacaatattc ttattgccct caaggtttaa ccgcttgagt attctgagaa
      241 ccctttcata ttcagagtca ttgaagtgca gtatgacctt gatagaagga tgagttgtat
      301 cactcgccgc ttcatatact ttacattggt tttgaaggtt tctctctaat tgtgtgtttt
      361 tagctaattt gaattctata attgttttat cttcagctcc ccgagaaatc ttaaaatcgg
      421 ctggtccacg tccatcatta gcttcacggg ttacgtccga ctgagtcgcg taccaggtta
      481 accgatacat aatctgtaaa tcagcttccc gttcaattgg gataccatta acgtaaaata
      541 tcttatgacc acctttattt tcaattacat ctttcagaaa cattactcgt tcgtgcgctt
      601 cttcgtaggt attgtatggc agacgataaa acccgcctgg atcaaggtat tgggatacta
      661 acgcacgaat tttgtctatg aatacagtct cgacctctcg tactcgtgct tctgcaaacg
      721 atacagcttg atctccacta tcctctttat cgcgaatata atagtcaagc acctggggaa
      781 atcgttctac cgcgctacca atagcttccc gaatctcctt ctgctttacc tttgggccct
      841 taggtattac tcttgctaaa tagtcgttaa cttgagcgcg tagcgttgca tcaggtaaag
      901 cgttcgctat ttccggaaaa cgatctagaa gctcgggacg gtttatccat gattcatctt
      961 tggtgagaat atctttgggt gttagtaaaa tgaaatcatc gtggatataa ggtagctcga
     1021 acgatgctgt ggtccatgac cgagtatcat agttgaattt tacttttctg agagtgacta
     1081 gtcgacgttg agaagggttt agatacttaa gtgcaaaatc ttgagtgaat tctgctaagt
     1141 agcacttgat taaattcatc gtgaaatcgc ttatattatc acgtccgact ccatctctta
     1201 ttaggcacaa tttctcaaga tggctgctcc gggttactac ttcatcccca aaatcacgaa
     1261 agacagtact taggtttcgg catagggcat ctgcgaagtc cattccgagg ccacgccctt
     1321 gattcccgac tctgcttaat ccaaaccagt tttgtttaat ctcggggaaa gtaaaccact
     1381 cctctattag aggtcgtgga actgtgcctg cgagtgaaac ttcttttaga aatctcatgt
     1441 aagtaataat ttgggcatga agctcctggt aagtgtcatc ttcactgtta aacaatagaa
     1501 aaggatcaat gaaaagaggc aggtcattta taagcgaaat atcaaaggct ccgtgttcct
     1561 cgacgacctc tggagaaact gaaaagtaat cggagaaata tatttctgtc atcttctttt
     1621 cgtcgcccaa cgctccgttt gacttcattg tttcatgcaa agactacttc ctgcggttta
     1681 tcatctttct gccgtgggga aatttgcact gtaactttac ggcctagttt gtttaaaaag
     1741 ccgaaaagcc gtccttctga aaacaaatga tatttgccat tcatcagctt ggacacctcg
     1801 gattggtcaa tcctgagcag ttcagaaatt tctttctgtt tgagaccgcg cttttccagg
     1861 attatgcgaa ctgtatggcc tagcttagcg cgtatcagta attcatctgc ttcatcaagc
     1921 ccgaggtcgg caaaaatgtt accgcttccc atttcatatt caggtttttt tctgctcata
     1981 ttgtttctcc atttcaaccg cctgttcata gcggctctta attaaatctt tgtccctttc
     2041 tgcggtttta atgccctttg gtgatttctt ctgaaatgcg tgaaggacat aaactctgct
     2101 gcctatctgc acggcgtaaa ccgttctgta ggtattggtg gcgtgacgtg tgacgatttc
     2161 aaagaccccc ctgccaacac ctttaaatgg ctttgcatct ggcggtgttg tccccatttg
     2221 cacgtcaaaa agagcaacac caatttcttt tttcacttct gtcggaaagt tcctcacgcg
     2281 cttgcgtgaa tctcccatcc agacaactgg ccgtgctttg acatcatcca atatacacct
     2341 ctattatatg agtaggtact cataatgtca agatgtaaat tctttcaggt taggacataa
     2401 ccacatcatc ataagctagg atgatagggt ctttggattt taagtcgtca caatcaatat
     2461 tattatcaca ggccgtagcg ccaggcgtgg cctgcggggt taagtagtgt ttaagtatca
     2521 aatttccgta tcgtctgtta tcacaccgtg gatgaaacat tagtagaaag cttgttctta
     2581 cccagaatcg gtaattatgt ttcagaagcc ttcctaacgg aaagtgtcgg gccaacgcac
     2641 agcgctatga ataacggcca caatctggat ttcagaatct cccaaaatgt aaaccacgat
     2701 gtatggtgat cctgctataa cgagttcacg cgttcctcgc actcgccctg gtcgtcccat
     2761 gtggggatgt ttgtttaaac gctgtgttgc gtcctgaatg cgcacaacca tgcggcttgc
     2821 tgctaaagga ttgtcctcag cgatatactc atgcaagtga cgcaagtcct gcagggcaat
     2881 gcgtgtccat ctcagtttca tgagggccgg ccgtatttat caaaaaaagc ctttacttcg
     2941 gcttcggtgg cgaaatctcc actgcgggcg gctttcaccc ctttccgaat atgggtcaat
     3001 tgccattcgt gaagctcgat gtactggtca agcgcctcat tgataacggc gctcaggttt
     3061 ttcccgaagg cttcggctat atgacccagg cgtttacgtt tgcctgcccg tgttataaag
     3121 gaaatccttg ttgaactttt tggcattaat aagctcctat atattacctt ataagatact
     3181 atcatatatt atattatttt taaaggtttt gtatacatgg gattgttaat tgtgttttat
     3241 gcaaagataa ttacctgtgg ggtgttgcct ttttcccttg aggaagtctg cactgtaaac
     3301 tttcgttcca gcatgttaaa aaaaatcgaa aggccatacg caaaaagaaa atatgtaacc
     3361 atcctaccct ttgtagaaac tattacgaaa atcaaagctc tgctaattcc ggcacctcag
     3421 ttaaaaatac accggagcta gaaagaagat ttattatttc tcttctaggc aaagaatcta
     3481 tgagaatatg tttagctctg ccgccaagag cgttttttag tctatggctt attgtaccct
     3541 ttcctgggtt ggataaggct tttgagcata tgttttcgat tccgttgtgt gtttcataaa
     3601 taacagtagg ctttacttca agaatatcct caaaaaaact atccctgttc tctgctatag
     3661 aaattcggtt tcccatgtag ataccaaggt aattcacaaa cctttcatat gggctgacat
     3721 gaggcagatg agaaaaggct tgatcctctt ctgtaatgaa taaaagcttc tccgatcctg
     3781 ctttaagagc tttgattaag tcttcatgcg ttatttcttt tcgttctttc tttaaatctg
     3841 aatcaaggtt gtaaaaaata gcagccgttg agcctggcaa aacaccttcg gctcttctaa
     3901 ccaactcatc ttcgagcacc tttcttttca ttgaaccgaa tttaagaaga cccttaaatg
     3961 gaataacttt ttcgtcctct cctataccag tatcagtaaa agttattatt ttatgcaaag
     4021 aaggaacctc gtggatcttg ttgctgagtt ccttcaaggt agattccttt gctacaaaca
     4081 tgaatctact ttttgaatct tttgcaatcc tgattatttt ttctagatcc tcattatcat
     4141 caatgggtat tgtaattccg ccgagaagat aaacaccaaa ttcagtaaaa agagactccg
     4201 ttcgacttcc tgagacaatt atagcggcgg tacctggagc aacgcccaag cccaaaagaa
     4261 aacaagcgat gtcctttgtt gttttttcaa agtcgagcca tgttatttgt ttccagctcc
     4321 aaccctcttt gtgctgcaaa aaaagccgac ccccatatct cttgatacgg tttgtcatca
     4381 tctcaggtat ggttttttca tcattcatgt tttaccctac aaatcgcttt aatcaaaata
     4441 aatcgtagta tactataaat gctcaaaata acactatgga caaaataact aaactcgaac
     4501 cgtttcgtgg tgaacaattc aattctgcct tcaatatagt cgagcgtctc agatcatccg
     4561 ggtataaagc attctttgtt ggtggatgtg taagagatac cgtgatggga attccgccta
     4621 aggaatacga tattgccact agcgctagcc cagaagagat tatgcgtctt ttttcaaata
     4681 ctgtacctat aggagcgagc ttcggcgtta tattagtcct tcaagatgaa tgcaaattcg
     4741 agctagcaac attcagacaa gatgaaagat atacagatgg aagacatcca gatagggtaa
     4801 tctacacatc ctctgaaaaa gaggatgtaa caagaaggga ttttactata aacggtatgc
     4861 tctacgaccc gataacagaa gaggtaattg attatgtcgg tggcattgaa gatataaaaa
     4921 atgggattat aagaacaatc ggaaattcat atgagaggtt caatgaagat aaactccgaa
     4981 tgataagagc catacgattc ggagcaaggt ttaactacga aatagaagag gaaacccttc
     5041 atgctataag aaagcttgca tatagaattt ccgatgtgag cactgagagg gtaagagaag
     5101 aaattgtaaa aattattact cagaaaaatc ctggtgtcgg tttattatta ctaagagaaa
     5161 ctgggctttt atctcatatg cttcccgagg ttgacaaaat gtttggaata cctcagcctc
     5221 cagaatttca tcccgaagga gatgttttta ctcatacctg tctcattctt gacaaagtct
     5281 ttgaaattac tgaagggaat acaacccttg aactttctgt aggaggtctt ctccacgatg
     5341 ttggaaagcc ggttacattt agtattgaag atagaatcag atttaatgca cacgatagaa
     5401 ttggagcgga aatggctgaa aagatatgca aaaggcttag gttttctaat aaagagatcg
     5461 aaagaatcaa ggctctagtt agtgaacacc taagattcaa agacgtattc aatatgaagg
     5521 aaagtacact aaaacgtttt cttggaatgc cctattttga agaccatctt atacttcatc
     5581 tagcagactg tcttgcaagt catggctcta tcgaagctta cgattttgtg aagaaaaagc
     5641 tcgaggggtt taaagaagaa gagataaaac caaagcctat tcttactggc tacgacctta
     5701 ttgaaatggg atataccccc ggaccgattt tctcagagat tcttgaatcc ctagaagagg
     5761 tccagcttga gggaactgtg aaagataaag aagaagcaaa aaagttcgtg ctggagaggt
     5821 tcccgatata agaacgagtt tctttaatta aaatagcaaa caaaaggcac tataaacata
     5881 taatatcaga tggtttctac cctcgatgaa gcggatcccg atggcttttg aggacttaaa
     5941 caatatacaa atggttttgc tcgttcccac accgaccaaa ggataggtaa ctttacactt
     6001 aattccatta acattagaag ataaccatat accaattcgg aggtaattcg ctacttaaaa
     6061 atctcgtcat caaagtccga atagatatac caacttttac cccttaaaat ccaggggtcc
     6121 tgaagtagga tggttttctc agaaaccgat gggtatttat aataacttat aagaacccta
     6181 actaaaccct tattctcaac tgagttaaat gtcacttctc taatttcgta atttgttatg
     6241 ttaagatcgt cctttgaatt taggagaaag ctttcaaatt tatccttttt ttcctcatct
     6301 ataaaaagag aggccctttc gtatgacttc cacataaata ggttgtaata gcttgaagct
     6361 gtctctttaa gtaattgttg gtttttctcc actgatcctg tggcatagca gcctagcagt
     6421 aagataggta aaaatactag gtacttagta atcttcctca ttttaatgga aatgatatta
     6481 taatttttag ggaaagtcta taaaaaaccc agaaaagtag ggaaacctcg tgtcaccccc
     6541 gatgaattct tcgggtgcat tcacaaggat tgcccctttt ttttatcttg aattaaatct
     6601 ttgcctgaag caaaaaagtg atagcaaggg cgtaaataac aagtgactca ataagagcta
     6661 aaccaatgat cataggggtt tgaatctttg ctgcagcccc tgggtttctt gcaataccgc
     6721 ttaaggctgc tgctgtagcg cgcccctgac ctaaacctgc tataccagcc gctataccaa
     6781 tacctaaacc gcttccaata gcgagaccaa acttcactaa atcacctagc tctttaaccg
     6841 gagcttcctc ttgtgccaat acttcaggaa cgaaagctcc cagaagagca aatccaatcg
     6901 tacacaatgt aaagagaacc cgtctcattt tttcacctcc cgatttaaat gatgcatgat
     6961 tcaagatgca cgatgcatga agttttgtat gtgtattgta tcctgtatca cgtaacctgc
     7021 atagtgtatc attccagatt ctgctaatgt tcatgcgccg ttgaaaggga aatatatatt
     7081 gctgaaagaa cagtaaaaat aaatgcctgc aaaaacgata caaaaatccc aagagcaata
     7141 aacagcgctg gcactacaaa gtgagtgagg ttcgtaaaaa ttcctagaac caaatggtct
     7201 cctgtcatat tccagaaaag tctcaacgaa agggataggg gacgtacaag atggcttatt
     7261 atttcaataa caagcataag cggtgcgata tagattattg gtccagcaaa gtgttttaag
     7321 taaccaatgc cgttttcctt aagcccatag aagttataca taataaaaat cacaagagcg
     7381 caggcaacag gtgtattaaa attcccagta ggcgaaagaa accccggtat taaacctaga
     7441 aggtttgaga aaagaatgaa cataaaactc gttccaagaa gagggaaata tgtctttgcc
     7501 ttttcacgag aacccatcat gcttgtaaag aggtcgagca gaaaatcaat tgtaagaatt
     7561 tcaaatatcg ttcttaatga aaatttctct tcaggaagaa cagcattctc attagaaagg
     7621 ctcttcttta tcttataacc agcaaaaatt aaaaaaagaa caactaaaat cgctcccaat
     7681 atgggatcgt atttgcctaa cccaaataag gtaaaccaac taaagtgctc catctttctt
     7741 tcctctaaga ccctccccaa taatgactat tactacgacc accatgccta taagaaaccc
     7801 atagatgttt atctttgtaa atgtaaaaag agaaattagc aacccaatga aaatagccat
     7861 tttaatcaaa agcacaaaaa cactaaaacc cttggaatga gcacctccta taagagctcc
     7921 tactactaag cgaatagcta aaaaattcac aattaccaat agtcccccta ccccaactcc
     7981 tagagcaatc tcacgtaagc caaggatggc atttatcgct acaagtattc ctgtaacgat
     8041 taacccatgc ttttcaaccc tactaaacgt tattgggctt gttgtcttca ttatttctgt
     8101 agtcattttt tcttttagtc atccttatga gtaagctaaa acccgctact acacctgcta
     8161 taagacctat gaatgtaaac catggtgttt ttgtcccaat ccatttatca acataatgac
     8221 ctgctataag ccctgctata acagaaaatg ccaattctgt accgatagca aagtatctta
     8281 gccacccaag atttttttct tccttaacct taacttgcct ccatatgaat cgtggctaat
     8341 gactagtggt ttgcgacctt gtcatgggcg cctagccgat gctgggctcg gccgaagcct
     8401 tagccgaacg attcgataca agattcacga tatactatcc accacttgct gtttctttaa
     8461 gcgctttata acaagcccta agcgtaatat taatttccct ttcagtgtgc gcaagtgaaa
     8521 gaaagactgc ctcaaactga gaaggcggaa acataatacc tgcacttaat aagtttttaa
     8581 agaacctgga atacattttt gtgtctgagt ttagtgcaga ttcataatca atcacttggc
     8641 ggtctgtgaa aaatagagta aacatcgagc ctacagagtt aatttgggct tttataccaa
     8701 gtttcttcag gatcttgtgc aaaccttcta taagactctg agttaattca ttcagtcttt
     8761 tgtatgttcc cttttccctg agtttattta tcgttgcaac ccctgccgcc atagcaacag
     8821 gatttcctga gagt
//
LOCUS       DATDRL010000334         5713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2107622, whole genome shotgun sequence.
ACCESSION   DATDRL010000334 DATDRL010000000
VERSION     DATDRL010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107622"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..321
                     /locus_tag="VI935_11125"
     CDS             145..321
                     /locus_tag="VI935_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26180.1"
                     /translation="MEKAIYVKASNEFVEMLKQSQWHLKMRPAQIVREAVLEYLERHL
                     PKEVKEEMLKKGGR"
     gene            324..1433
                     /locus_tag="VI935_11130"
     CDS             324..1433
                     /locus_tag="VI935_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HLE26181.1"
                     /translation="MGVYQKGNRWFIDYYLLDGRRKREAVGHVDKISRSLAEKALKAR
                     VGDIVQGRYNLEGTKKPLRMNVLFDRFLEWAKTNHKNPDKTASAVKPLRAFFGDRLIS
                     EMNLWLVDRYKAHRKTEAKKPETINRELGILRRMLNLAVEWKLIVATPIKGLTLVRVT
                     KYLPRVLSVEEFERLYSSASVHFKPVLLCAFLTGMRKGEISRLRWEDVSLKDGYLTVK
                     ESKNGESRTIPLHSRLISTLIELKDRSENPYVFTTPEGNAYISDSAWKRAFYTALKKS
                     GIGACRFHDLRHSFCSNLIVGEKEDFVTVMELTGHKDMRMLKRYSHTKESAKRIAIDK
                     LGNRLNQIVIDTPLDTTPKITNATLANLSVVSSRV"
     gene            complement(1446..1522)
                     /locus_tag="VI935_11135"
     tRNA            complement(1446..1522)
                     /locus_tag="VI935_11135"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1486..1488),aa:Gly,seq:gcc)
     gene            2012..3160
                     /gene="proB"
                     /locus_tag="VI935_11140"
     CDS             2012..3160
                     /gene="proB"
                     /locus_tag="VI935_11140"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HLE26182.1"
                     /translation="MKKTDRKTFLSQAKRVVIKIGSSVLTNEYGVLSEGVFDEIAGEI
                     SRIKSRGIEPIIVSSGAIAAGMKKLNLGVKPRDISMKQAIAACGQSALMWYYEKAFLA
                     FGEKVAQILLTHDALSVRKRFLNARKTLFTLLGMGVIPIVNENDTVAVEEIMLGDNDN
                     LAALVTSLVEADLLILLTDIEGLYNKDPRNSRDAELITLIEEVDERIEEIAGETLGRT
                     TTGGMRTKIEASKTAAAFGVPTIIANGKKPTNLSEIFSGKDVGTLFLPAKEKLKGRKH
                     WIAFSLKPSGEIRIDDGAKRAISSSGKSLLPAGIKDVSGEFEVGELVRCIDDNGMEIA
                     RGLSSYSSDEIRKIAGAKTSEIEKILGYKYSDEVIHRDDLVVVSKKDL"
     gene            3437..4429
                     /gene="mltG"
                     /locus_tag="VI935_11145"
     CDS             3437..4429
                     /gene="mltG"
                     /locus_tag="VI935_11145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HLE26183.1"
                     /translation="MKLTANIKIGIAVMLIALVTAFVYFAYFTPLSSQKAEKVVLEIP
                     RGLGLKDIALKLEEERVIRSDKLFILISFIKGASGKLKAGEYEFQIGDPIDRIIDKLI
                     NGDVVVRRITIPEGFTTNDIALLLEKNNVMHSESFVEKANSNELTKELFGVPLPSFEG
                     YLFPDTYLYKKGISPEELIRMMVFRFKEVYEPLKANSGELNLKDHEVVILASIIEKET
                     GNTYERPLISAVFHNRLRLGMRLESDPTVIYGMSGFDGNLTKNDLKIETEYNTYTIIG
                     LPPGPISNPGKDSLIAALNPANANYLYFVSKGDGSHEFSSNYLDHQRGVTKYQR"
     gene            complement(4450..>5713)
                     /locus_tag="VI935_11150"
     CDS             complement(4450..>5713)
                     /locus_tag="VI935_11150"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HLE26184.1"
                     /translation="LKYMTAFRVPEEIFLKIDEILEDVISKKKLNRDPKSNIESPNSE
                     FDRHAPGAKEKESFGGEERPSDQLSEARKTFFQALKELKENLAGERNNMIMHPEQDAQ
                     TKPSGDSDDEGQDGGHRQNSHHKVYGSKSPRAGDEDDTWIGTGKRNVESAENVKIVMR
                     VLEGNIQRSIALDAPHPGGQPRRWKRMNHFEELEDVDPFEGMFWAEYTTPSLPRVHKR
                     EKEMLGGEIAILREVSTSMMGVYSEWSSCVVRAVVELSRAKKMRVGYIEFNHRSYKYK
                     RVGKFFTRDYEWMLQLASKTECSGNTNYEDALKDALSEFKGRGLRNKHILFITDGIPT
                     SGDCEVLNERTRAKKLGVCIHSIFIGSKNYPKILQKISEESSGAQFTASRGKDGTIRI
                     ERKEKKLSLPTTNIGKPRVDPFTDVFSQ"
BASE COUNT         1701 a         1118 c         1279 g         1615 t
ORIGIN      
        1 ttattggagt tacgttgtcc gactgtctgt acatatgtaa agggtgttta ctattcttta
       61 cagaaatagt attgactaaa tatattaact atgttatata ttaataatat gatacaagag
      121 aaaccgaagc gatggagaaa acctatggag aaagcaatct acgtaaaagc atctaacgag
      181 tttgtagaga tgctcaaaca atcccaatgg catctgaaga tgagacccgc tcagatagta
      241 agagaggctg tattagaata tcttgaaagg catttgccga aagaagtaaa ggaagaaatg
      301 ctaaagaaag gaggtagata atcatgggag tataccaaaa gggtaaccgt tggtttatag
      361 actactatct actggatggt aggcgaaaac gggaggctgt agggcatgta gataaaatta
      421 gtcggtcttt agcggagaaa gcacttaagg caagagtagg ggatatagtt caaggtagat
      481 ataatctgga agggactaag aagcccctgc ggatgaatgt gctgtttgac agatttctgg
      541 aatgggcaaa aaccaaccac aagaaccccg ataagaccgc ctcagcagtt aaacctttgc
      601 gcgctttctt tggagaccgt cttatttccg aaatgaatct ctggttagtg gatagataca
      661 aagcgcacag aaaaactgaa gcgaaaaagc ctgagaccat taatagagag ttaggtattc
      721 tgcgccgtat gttgaatctc gctgtggaat ggaaattaat tgtagctact cctataaaag
      781 gactaacgct tgttagagtg actaaatatc ttcctagggt tttgtctgtt gaagaattcg
      841 agaggcttta ttcctccgca agtgttcatt ttaaacccgt tctcttgtgc gcctttctaa
      901 cgggaatgcg aaaaggcgag atttctaggc tcagatggga agacgtgagc ctgaaggacg
      961 gttatctgac tgttaaagaa agcaaaaacg gtgaatcccg aacaatccct ctacattcgc
     1021 gtcttatctc aaccctgata gagctaaaag acagatcaga gaatccttat gtctttacta
     1081 ccccagaagg taacgcctac atctcagatt ctgcatggaa aagagcgttt tatacagcat
     1141 tgaaaaagtc agggataggt gcttgtcggt ttcacgacct acgtcatagc ttttgctcta
     1201 acctgattgt tggagaaaaa gaggattttg tgaccgtcat ggaactcacc ggacacaaag
     1261 acatgagaat gctaaaaaga tattctcata caaaggaatc cgcaaaaaga atcgcaattg
     1321 ataagctggg caatcggcta aatcagattg ttatagacac ccctttggac accacaccaa
     1381 aaatcacaaa cgccacactt gccaacttat cagttgtaag ctcacgagta taaatgatta
     1441 ttcgctggag cgggaaacgg gattcgaacc cgcgaccctc tccttggcaa ggagatgctc
     1501 tagccaactg agctattccc gctcttagtg cctttaaaga tagcaagagc aaagtgtatt
     1561 gtcaataagt tttaccgttt tatcgttcgt caacccaagc ttactagact taaatttcta
     1621 agatgggtgt aaacttttgt atgagcccgg cggagatatt attcccgtat aaacagcggg
     1681 cgggctataa acctgtcggt agggttgaaa ctccttcgat aagctcaggg ggataagcat
     1741 gggatgataa actgatttta tcgaggctag aagcctctcc tacaaggagc ctatggcaga
     1801 catgtattgt atgagtgcct tcagggcgca ttagcatccc cactttaatt atccgcgact
     1861 ggaaagccac ggctatcgaa ggccaatttt cacacaatca caccatgata tccgaggttt
     1921 tctaacctcg gataaaggag cacgattatg gtcaacgagt ctcaactatc ccgcgctttt
     1981 aaggtaatat aataatttac cttaaaaaat tatgaaaaag acagatagaa agactttttt
     2041 atcacaggct aagagagtag taataaagat aggaagtagt gttcttacaa atgagtacgg
     2101 tgtgctttct gaaggtgtat ttgatgagat tgcaggagaa atttccagga tcaaatctag
     2161 ggggatagaa cctataatag tttcttctgg agctatagct gctgggatga aaaagctaaa
     2221 tttaggggtg aagccgagag atatttcaat gaagcaggct attgcggcct gtgggcagag
     2281 cgctcttatg tggtactacg aaaaggcttt cttggctttt ggcgagaagg ttgctcagat
     2341 tctgcttacc cacgacgccc tatctgtaag aaaaaggttt ttaaacgcac gtaaaacgct
     2401 atttacgctt cttgggatgg gggtaatacc gattgtgaat gaaaacgaca cggtggctgt
     2461 tgaagagatc atgcttggag acaacgataa ccttgctgct cttgttacaa gccttgtcga
     2521 ggcggatttg cttattctcc tcacagatat tgagggtctt tacaataaag accctagaaa
     2581 cagtagggat gcagaactaa ttacattgat agaggaagta gacgaaagga ttgaagaaat
     2641 agcgggagaa acattgggaa gaacaacgac tggaggtatg aggacaaaga ttgaggcatc
     2701 gaaaaccgct gcagcattcg gtgttcccac gattatcgca aacggaaaaa aaccaacaaa
     2761 cctctctgag atcttttctg gaaaagatgt aggaaccctt tttctgccag caaaagagaa
     2821 actaaagggg aggaagcact ggatagcttt ttctcttaaa ccctctggag aaatcagaat
     2881 agacgatggt gcaaaaaggg ctatttcatc ctctgggaaa agcctactcc cagcgggaat
     2941 aaaggacgta agtggggagt ttgaggtagg tgagcttgta aggtgcatag acgataatgg
     3001 aatggagata gcaaggggcc tttcttccta cagctcagat gagataagaa agatagctgg
     3061 ggctaaaacc tcggagattg aaaagatttt agggtataaa tacagtgatg aggtaatcca
     3121 tagagatgat cttgtagtag tatcgaaaaa ggatttataa gtaacgcaca gcttctagta
     3181 attgtcattg cgagcgtcag cgaagcaatc ctttgattat ttttgagatt gcttcgggac
     3241 ggagcctgca ccgagcgtat gcgaagtgtc cctcgcaatg acagacaaag tgtcttgctc
     3301 atcttaataa catcaaatta tggattgttt gtaggatact ataaatattt ctttcgaggc
     3361 agtaataaat tacggctcac aaactcctcg agttgtgaga attaatattt tggatcagaa
     3421 ctgaaaagct aagagaatga aacttacagc aaatataaag attggaattg cagttatgct
     3481 gatagccctt gtaacagctt tcgtttactt cgcctacttt acccctctct cttctcagaa
     3541 ggctgaaaaa gttgtcttag aaattccacg gggtctagga cttaaagata tagccctcaa
     3601 gttagaagaa gaaagggtaa taagaagtga taagttattt attcttattt cttttataaa
     3661 aggggcttca ggcaaactta aagcaggcga gtatgaattt caaattggcg atcctataga
     3721 tcggataata gacaagctaa taaatggtga tgtggttgtt aggagaatta ccatccccga
     3781 aggttttaca acaaatgata tagctttact cttagaaaag aataatgtta tgcatagtga
     3841 gtcgtttgtt gaaaaagcca atagtaatga gcttacaaaa gagctttttg gggttcctct
     3901 cccaagcttt gagggttacc tctttccgga cacttatctt tataaaaagg gaatcagccc
     3961 tgaagagctt atccgcatga tggtattcag gtttaaagag gtttacgagc cacttaaggc
     4021 aaattcaggg gaactaaatc ttaaagatca cgaggttgtg attcttgctt caattattga
     4081 gaaggaaact ggaaacacat atgaacgccc attaatttcg gcagttttcc ataatcgcct
     4141 tagactcggt atgaggcttg agagcgatcc aactgtaatc tatggtatga gtggtttcga
     4201 tgggaacctt acaaagaatg atttaaaaat agagactgaa tataatacgt atacaattat
     4261 agggctcccg cctggaccga tatcaaaccc cggtaaagat tcccttatag ccgctttgaa
     4321 cccggcaaat gcaaactatc tctatttcgt atcgaaggga gacggcagcc atgaattttc
     4381 ttctaactat ctagatcacc aaaggggtgt aacgaagtat caaaggtagg ggtgtattgc
     4441 aatacgtccc tactgagaaa atacgtcggt aaatgggtct actctaggtt ttccaatatt
     4501 agtagtgggg aggcttagtt ttttctcctt tctctctatc cttatcgtgc cgtctttccc
     4561 tctcgatgcg gtgaactgtg cgccgcttga ttcttcagag atcttctgaa gtatctttgg
     4621 atagtttttt gatccaatga atattgagtg gatacaaaca ccgagctttt ttgctcttgt
     4681 tctttcgttc agaacctcac agtcacccga tgtaggaatt ccatctgtaa tgaaaaggat
     4741 atgcttattt ctaagacccc ttcccttaaa ttcggagagg gcatccttta aagcatcttc
     4801 gtagtttgtg tttccggagc actctgtctt cgacgctaac tggagcatcc attcgtagtc
     4861 tctagtgaag aattttccaa cccttttgta tttgtaagat cggtggttga actcaatgta
     4921 tccgaccctc atcttcttag ctctagatag ctcgaccaca gcacgtacaa cacatgagga
     4981 ccactcacta tagacaccca tcatagatgt gcttacttct cgaaggattg caatctctcc
     5041 ccctagcatc tctttctcgc gcttatgcac ccttggaaga gaaggtgttg tatattcagc
     5101 ccagaacatt ccctcaaatg ggtcaacatc ctctaattct tcaaagtggt tcatcctctt
     5161 ccatcttctg ggctgtccac cggggtgagg cgcatcaagg gcgatgcttc tctggatgtt
     5221 tccttctaaa accctcatta cgatttttac attctctgca gattcgacgt ttcgttttcc
     5281 agttccaatc catgtatcat cctcatcacc tgcacgcgga gacttacttc catatacctt
     5341 atggtgacta ttttgtctgt gacctccatc ttgaccctca tcgtctgagt ctccactcgg
     5401 cttagtctga gcatcttgct caggatgcat gatcatatta ttcctttctc ctgcaagatt
     5461 ctctttgagc tcttttaggg cttgaaaaaa ggttttacga gcctctgata actgatcgga
     5521 tggacgttcc tctcccccaa agctttcttt ctcttttgca ccgggagcgt gcctatcgaa
     5581 ttcagagttc ggtgattcta tattgctttt tgggtcccga ttgagttttt ttttagagat
     5641 cacgtcctca aggatttcat caatcttaag gaatatctct tcgggtacac ggaaggcggt
     5701 catgtatttt agg
//
LOCUS       DATDRL010000335        10725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3556588, whole genome shotgun sequence.
ACCESSION   DATDRL010000335 DATDRL010000000
VERSION     DATDRL010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10725
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3556588"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1072
                     /locus_tag="VI935_11155"
     CDS             188..1072
                     /locus_tag="VI935_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HLE26185.1"
                     /translation="MELMEAIGSRRSIRFFKPWRPVEEWKIQMMLQAARYASCQGNCG
                     STEAIVIDKKTYPKDKLEKIIECASPFNEIHLRQAPVVIAWLLNLDAWYKELVQSFAV
                     LFPARAVSAAHGWTYKLLTEVTYPRLMSFPRERTEALLRIEAGQAIANAFLVATDLGL
                     GCCLLATGRKPAEFPKALGVPDNVVPIWLMAVGYSAESPGQRPRKRFDRLFHLNEYGN
                     PLKEDPTVKEELKRRKLIQPMDPLPGRDEEVQSICRMFGFSEDLPDMPKEHIRALYED
                     EKSPYYGELPPGLEEKGV"
     gene            complement(1289..2527)
                     /locus_tag="VI935_11160"
     CDS             complement(1289..2527)
                     /locus_tag="VI935_11160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HLE26186.1"
                     /translation="MNLKINSLKKFDSIFGKLSCSFVKFFVKPRKGLDCMYKNTQPQH
                     GFDLQPESDGSGLSAISEIAPHFGTKALSKKIKILVIRPGGVGDSALLYPAFKVLKES
                     FSRSEIEVLAEKRNAGLLSSCPYINRIFLYDFRFPIELFKTLRISYDIVIDTEQWHRL
                     TAAVSYLTRAPIRVGFATNERGDLFTHPVSYNQDEYEVYSFLNLVSAITGEGYDFNEN
                     EPFIPLDSDLISSIEPKVTEFKKRWLALIGVFSGATIPERRWGTTNFAELSKRLSQEG
                     LGIVLVGGKYEVKDAIRFEEILGRDKVLNFVGETSLMETAAIISKLDLFVSGDTGIMH
                     IAYGVGTPTVSLFGAGIQKKWAPIGKRHIAINKNLSCSPCTKFGYTPSCPYEVKCLRD
                     ISVEEVKEVALRLLSQCKNE"
     gene            complement(2508..4256)
                     /locus_tag="VI935_11165"
     CDS             complement(2508..4256)
                     /locus_tag="VI935_11165"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLE26187.1"
                     /translation="MWLIERKAFASLFLFFVSFGVFAFSLKNDFVWDDVQVIEKTYYS
                     FKASRIKSIIIPKIKRSQNLAPYYRPIIFISNVLDRSLWGISPFGFHLSNVVFYSIST
                     VTFYFLMLIVFGEFSVDQKEIKAFLSSLLFTLYPMHVESVSWVAGRTDLLSGLFFFIA
                     FIFHIFSFRKLWFVTLAAFSFFLSLLSKEVAIVFPIVALGFDLLSRKFVIRNFTLKYT
                     FYALLILLYFYLRGRAFDSVPDLISQSSTSSTYEGSQILGHLKILLNSYLFYIYKLVF
                     PFEFNAFIGSVPNDIYFFISSILVIFLLCVISVISIIKKKYVNPFGIFWVCVTLGPSC
                     LVAISVFSSTPLAERYLYIPSAGYCLLVGHFIFEVGKRINFQKAALALGFLLSFSYLF
                     FTIDRQKVWKEDLILWEDTSSKATNYAIPHSNYGMALKDAGKKDEAIRELLTALDPEV
                     KDTKRGRATTANNLGLVYLDKEDYKNAEEWFLKALYYDSTYGRTYYHLGLIYFIKGEY
                     GNSVSDYRMAEKYLKKTLEMYRSYGRANLLLAKVYIRLGEREKARENAERAIRSGLIE
                     PLSIEAQGILNELKDK"
     gene            complement(4548..5612)
                     /locus_tag="VI935_11170"
     CDS             complement(4548..5612)
                     /locus_tag="VI935_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26188.1"
                     /translation="MFTSLFKGPVKWLILVFALIAISVPFQKGIDNLRGRFRSIEETL
                     YFTSSALKRLSLGYKELLADIYWMRAIQYFGGRRFKEKNPALLYHYFDILTDLDPKFV
                     NAYRFGGTFLSEPPPFGLGNIKKGTMLFDKGRRNNPENFRLPLEEAFIYYLYVKDYDK
                     AAELFNEASEKPSLSDLRRAHIKGMAASAHFKGGNRELSREIWKIIYETTTNEHRREF
                     ALKNLKELDTMDTEDRYTEALREYIERYNEIPTSLVALRDAGIIKQIPKEPYGGEFII
                     VSKLKAVRSSTLVNQQLRYNLIFLTAKARRFRYLYGRFPKDLAELKGFIVNETTADFP
                     PNPLGEEYVYNPENGIVESK"
     gene            complement(5790..6593)
                     /locus_tag="VI935_11175"
     CDS             complement(5790..6593)
                     /locus_tag="VI935_11175"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLE26189.1"
                     /translation="MKRIWSVAHNTFIEAIRDRVLFSLILFAFVMILSSLIFASISAE
                     QYNKIVKDLGLTAISLIGIMISIFLGMGLVYKEIEKKTVYNIFSKPIRRYEFILGKYF
                     GLSFTLLVNTAAMAFILFLLVLYTEMRHAGFIKFYYGGHYFAEFFKAVYFEYLEFIVI
                     IGIALVFSSFTTPVMSVLFTFFLFAIGRFSSDIRLLAEQVKNPVVGFITEVIYRVIPN
                     LEKFDVRREAVYGGSVSAELILYTTAYAILYVLALLVLSIIIFEKREFK"
     gene            complement(6685..7065)
                     /locus_tag="VI935_11180"
     CDS             complement(6685..7065)
                     /locus_tag="VI935_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HLE26190.1"
                     /translation="MKKPQSYKDLEVYSPAKEFAVKVHRLTIDKLPKFEMYEEGGQIR
                     RSSKSIVSNVVEGFGRRRYKNEFIQFLTYAIASCDETKVHLEILYETGSLKKEVFEEL
                     FHAYEELGAKLFNFREAVIRSHRV"
     gene            complement(7153..8100)
                     /locus_tag="VI935_11185"
     CDS             complement(7153..8100)
                     /locus_tag="VI935_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLE26191.1"
                     /translation="MLKIENLVKDYTSGFLRKKVRVLNDVSFSVNKGEIFGFVGPNGA
                     GKTTTFKVILGFVSLTGGRIEIMGKPMGDVDVKKHIGYLPENPYFYDYLTGEELLDYM
                     GELHGVDGKGLAKRVDELLEKVKMSHARRLQLRKYSKGMLQRIGIAQALINDPDFLIL
                     DEPLSGLDPMGQREIRELILEVKRVGKTILLSSHILSDVEALCDRVGVIIGGRVVKTG
                     ELAKLFEGIHTDYEMLLKGVDEKIVNYFTDLKVAVEKRTGFFVLKFDEDIKAKVLEIA
                     SQCRADIISLHPLRKSLEGLFVEEAKKDNTNNLTPKMRG"
     gene            complement(8704..9399)
                     /locus_tag="VI935_11190"
     CDS             complement(8704..9399)
                     /locus_tag="VI935_11190"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HLE26192.1"
                     /translation="MLKFKRGRKDEKGFTLIELLIVAAIIGIILAIAVPNLIKARISA
                     NEANARKAMQTLRDAEGEFFEQDIDDNGIRDFTDLIGEDPVATDGSLRCPDDTIAGGA
                     TCLESDALVDNSFVGARSEADGAPAALADCGNSKAGYCVAWTNDSTDTDLDGADWSDP
                     PGAILPDTLLQADFGWEASPSGVLKTGRRDYGVWGDGVIRCTTSQQASGVNGTYQATR
                     DGSQGGVPAGACD"
     gene            10383..>10725
                     /locus_tag="VI935_11195"
     CDS             10383..>10725
                     /locus_tag="VI935_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26193.1"
                     /translation="MNKKIYSVSFLFIFFLLCLSITGCGDLKVASYFNPSDPKLNKYK
                     TLEIADFETEITDVPNEALTRISDEVAKLTVSKNIGFQKVERGSTEGDPTQQTLVLVG
                     EITGYESGKSFK"
BASE COUNT         3343 a         2283 c         1949 g         3150 t
ORIGIN      
        1 aatctgcgat gaattagatg ttattaaggt gattagagaa caagtggatg cagaaacaat
       61 cttggctgaa gatttattag aggtagaaat ctaattgtag agacgacccg ccgggtcgtc
      121 tctacaataa aaaccttgac attaagacca caaactgctt taattggaaa gtaaggaggg
      181 taaaaatatg gaacttatgg aagcaatagg atcaagaaga agtataaggt tttttaaacc
      241 atggaggcct gtggaagagt ggaagattca gatgatgctt caagccgcac gatatgcgtc
      301 ttgccagggc aattgtggtt caactgaagc tatagtgata gataaaaaaa catatccaaa
      361 ggacaagctt gagaagataa tagaatgcgc ctcaccgttt aatgaaatcc atcttcgcca
      421 agccccggtt gtgatcgcat ggctcttaaa cctcgacgct tggtataagg aactagtcca
      481 gtcctttgca gttctatttc ctgcaagagc agtaagcgcg gctcacggct ggacatataa
      541 gttacttaca gaggttactt atccacgtct catgagcttt ccacgagaaa ggactgaagc
      601 gctccttaga attgaggcag ggcaagcgat agctaacgca ttccttgtag caaccgacct
      661 cgggcttggg tgctgccttc ttgcaactgg aaggaaaccc gctgagtttc caaaggcgtt
      721 aggtgtccct gataatgtcg ttccgatttg gcttatggct gttggatatt ctgctgagtc
      781 accagggcaa agacctagga agcgttttga taggttattt cacctgaatg aatacggaaa
      841 tccacttaaa gaagacccaa ccgttaaaga agaactaaaa aggagaaagc tgattcagcc
      901 catggacccg cttccaggaa gagatgaaga ggtacaaagc atctgtagaa tgtttggatt
      961 ttcagaggat cttcccgata tgcccaaaga gcacataaga gccctatacg aagatgaaaa
     1021 gtctccgtat tacggtgaac ttccacctgg actcgaagaa aaaggggttt agaaggtata
     1081 acgtcattgc gaggagcgaa gcaacgaagc aatctgcacg atgtatcatt ccgaatcctg
     1141 ccctgagtct aatagaatgg tatctcctgt gtaaaggcga atgagcagtg aggaatccca
     1201 aacagattgc ttcgcggagt ttacactcag tttatcgaag tgttcgcaat gacaattgtg
     1261 tatagtttgc aatttcgatt aatttgagtt attcattctt gcattgagac aataacctca
     1321 gcgccacttc ctttacttcc tcaactgata tatctctaag gcatttgacc tcataggggc
     1381 aactcggtgt gtagccgaat tttgtgcaag ggctgcaaga tagatttttg tttattgcaa
     1441 tgtggcgttt acctataggt gcccatttct tttgaatgcc agccccgaag agcgaaaccg
     1501 ttggtgttcc aactccatac gcaatatgca tgattccggt gtcgcctgag acgaataaat
     1561 cgagtttgga gattattgcc gcagtttcca taagagatgt ttctccaaca aaattaagga
     1621 ctttatctct cccaagaatt tcttcaaacc ttatcgcatc tttaacctcg tacttaccac
     1681 caacgagtac tattcctaaa ccttcttgag ataatctttt agaaagctct gcaaaattag
     1741 ttgttcccca tcttcgttct ggaattgttg ctccagagaa cactcctata agggccagcc
     1801 atcttttttt aaattccgta accttgggct ctatactaga aattagatcc gagtcaagag
     1861 gaataaatgg ctcgttttca ttaaaatcat atccttctcc tgtaatcgct gaaaccaaat
     1921 tcaagaagct gtaaacctca tattcgtctt ggttatatga aactggatga gtgaagaggt
     1981 ctcccctctc attggtagca aatccaactc ttataggtgc tcttgttaaa taagacactg
     2041 cggcggtaag gcggtgccat tgctctgtat ctatcactat atcgtaactt atcctgagcg
     2101 ttttaaaaag ctcaatcggg aatcgaaagt catagagaaa tatcctgttg atatagggac
     2161 aactcgaaag tagccctgca tttctttttt cagcaagaac ctctatttca gaacgactaa
     2221 agctctcctt taaaaccttg aaagcaggat aaagaagtgc ggaatcacca accccccctg
     2281 ggcgtattac aaggattttt attttttttg acaatgcttt agtaccaaaa tggggagcga
     2341 tttcgctgat ggctgataat ccactgccat cgctctcagg ctgaagatca aatccatgtt
     2401 gcggctgtgt atttttgtac atgcaatcta aacctttcct tggttttaca aagaacttta
     2461 caaatgaaca tgaaagtttt ccaaagatgc tgtcaaattt ctttaaacta tttatcttta
     2521 agttcattta aaatgccctg tgcttctatg cttaatggct caataagccc acttcgaata
     2581 gccctttcag cgttttctct tgctttttcc ctctcaccaa gccgaatata aacctttgct
     2641 aaaagaagat ttgcccttcc ataagagcgg tacatttcca acgtcttttt caaatacttt
     2701 tctgccattc tataatcaga aacagaattt ccgtattccc cttttataaa ataaataagc
     2761 ccaaggtggt aatacgtcct tccatacgta gaatcatagt aaagagcctt aagaaaccac
     2821 tcctctgcat tcttatagtc ctctttatcc aaataaacaa gcccaagatt atttgccgta
     2881 gtagcccttc cccttttggt gtcctttacc tcaggatcaa gagcagtcaa aagctctcga
     2941 attgcctcgt ctttctttcc agcatcttta agtgccattc catagtttga atgagggatt
     3001 gcataattag tagcttttga tgatgtgtct tcccagagga ttaagtcttc cttccaaact
     3061 ttttgcctat ctatagtaaa aaacagatac gaaaaagaga gaagaaaccc aagcgccaaa
     3121 gctgcttttt gaaagtttat cctttttcct acttcgaata taaaatgacc aactagcaaa
     3181 cagtatcctg cagaagggat atataaatat ctctccgcaa gaggagttga ggagaaaaca
     3241 gagattgcca caagacaaga aggaccaagg gttacacaaa cccagaatat accgaaggga
     3301 ttaacatatt tcttctttat tattgatatg acactaatga cacatagaag aaaaattaca
     3361 aggattgagg aaatgaaaaa ataaatatca tttggcacac tacctataaa cgcattgaat
     3421 tcaaacggaa aaacaagttt atatatatag aagagatatg aatttagaag tatcttaaga
     3481 tgacctaaaa tttgggaacc ctcataagta gaactggtag agctttgaga tatgagatca
     3541 ggaacgctat caaaagccct tcctctcaga tagaaataaa gcaaaatcaa caatgcataa
     3601 aaagtgtatt ttaaggtaaa attacgtatc acaaatttac gactaagtag atcaaaacct
     3661 aaagcaacaa taggaaaaac aatagctact tcctttgaaa gaagtgagag gaaaaaagaa
     3721 aaagcggcta atgtcacaaa ccacaacttt ctaaatgaaa agatatgaaa gatgaaagct
     3781 atgaaaaaaa acagaccact taacaaatcc gttctccctg caacccaaga aaccgactcg
     3841 acatgcattg gataaagtgt aaataagaga gaagagagaa aggctttgat ttctttttga
     3901 tcaacactga attccccaaa cacaatgagc attaaaaaat agaaggtaac agttgatata
     3961 gaatagaaaa caacattact caaatgaaat ccaaaaggtg aaattcccca taaactacgg
     4021 tctagaacat ttgagataaa gattatagga cggtaatagg gcgctaaatt ctgactcctt
     4081 tttatcttgg gaattattat tgattttata cgggaggctt tgaaggaata ataggtcttt
     4141 tctattacct gcacatcgtc ccagacaaaa tcgtttttta gggaaaaggc aaatacacca
     4201 aaagaaacaa agaacaaaaa aagcgaagca aaggctttac gttcaatcag ccacatagaa
     4261 taattatagt attggaatta cttataacaa aatgaagaac accgcagcaa gttagaaggt
     4321 atggtcttaa tattaaagcg ccacaactgt cattgcgcgg aaccctgagc aaagtcgaag
     4381 gggacgaagc aatctgacac gaagtgtcat tccgaacaga gtgaggaatc taagatttct
     4441 cgctttgaaa tgacaagaaa aagaatattt accatgacgc aatgatgaca gcgtaaacca
     4501 tgtggcaagc tacaggaaat tatcaaatta aaaattatag ttagaattta cttagattca
     4561 actatgccat tttctgggtt ataaacgtat tcttcaccta atgggttagg cggaaaatct
     4621 gcagtggttt cattcactat aaaccctttt agttcagcta gatccttagg aaaccttcca
     4681 tataaatatc taaacctgcg ggcttttgct gttaagaaga tgagattata tctaagctgc
     4741 tgatttacca aagttgagct tcgtacagct tttagtttgc ttactataat aaattcccct
     4801 ccataaggct ccttaggtat ctgttttatt atgccagcat cccttagggc gacaaggctt
     4861 gttgggattt cattataacg ctcaatatat tcccttaaag cctcagtata acggtcttcc
     4921 gtatccattg tgtcgagttc ttttaggttt tttaaagcga actcccttcg atgttcattc
     4981 gtagtggttt cataaattat cttccaaatc tctcttgaga gttccctatt tccgccttta
     5041 aaatgcgctg aagccgccat ccccttaatg tgtgcgcggc gaagatccga aagactaggt
     5101 ttttctgagg cttcgttaaa tagttctgcg gctttatcat agtctttgac ataaagataa
     5161 taaataaagg cctcctcaag cggcaggcgg aagttttctg ggttattcct ccttccttta
     5221 tcaaaaagca ttgtcccttt cttaatatta ccaagaccaa aaggcggcgg ctcagaaagg
     5281 aatgttccgc caaatctata agcgtttaca aacttgggat cgaggtctgt aagtatatcg
     5341 aagtagtgat aaagcagcgc tggatttttt tctttaaaac gcctgccgcc aaagtactga
     5401 attgccctca tccaataaat gtctgcaagc agttccttat atccaagcga tagcctctta
     5461 agagcggatg aagtaaaata gagagtctct tcgattgacc tgaaccttcc tcttagattg
     5521 tcaatccctt tttgaaaagg aactgagata gcaattaatg caaacacgag aattagccac
     5581 tttactgggc ccttgaagag tgaggtaaac atcggtatca caccatttct atcaaaatag
     5641 tggtgcattc aaaaagaatt gtcattgcga gcgtagcgaa gcaatctcct cttttaagac
     5701 taagtggcca cttagtattg agattgcttc gtcgctctgc tcctcgcaat gacaggcttc
     5761 cgtgtgcacc taaaaaaatt gaattgctat tatttgaatt ctctcttctc aaatattatt
     5821 atggatagca caagaagtgc cagcacataa agtatggcat aagcagttgt atatagaata
     5881 agctccgcac ttacactccc tccgtaaacc gcctcacgtc ttacatcaaa tttttccagg
     5941 ttaggtatta ccctataaat aacctcagtt ataaacccta ctactggatt ttttacctgc
     6001 tcagctaaaa gccttatgtc acttgaaaac cgtccaattg caaaaagaaa aaaggtaaaa
     6061 agtacactca taactggggt agtaaagctt gaaaaaacaa gagcgattcc aattatcact
     6121 ataaattcaa gatactcaaa atagacagct ttaaaaaact ctgcgaaata atgccctcca
     6181 taataaaact ttataaaccc tgcatgtctc atctccgtat atagcactaa aaggaagagt
     6241 ataaaagcca tagccgccgt attaacaaga agcgtaaagc tgagtccgaa atatttccct
     6301 aaaataaact catatctacg aatgggtttt gaaaatatgt tatataccgt ttttttctca
     6361 atctctttat agacaagtcc catcccaaga aatatagaaa tcataatccc gatgagtgag
     6421 attgccgtaa gccctagatc ctttacaatc ttgttatact gctcggcaga gatgcttgca
     6481 aagataagag agctaagaat catcacaaaa gcaaagagaa ttaaactaaa aagaaccctg
     6541 tctcttatcg cttcgataaa tgtgttatgt gctacactcc aaattctttt catttatttt
     6601 tgccactaaa tacatgattc atgatgcaag atacatgata caggatgtat gaaagttgtt
     6661 acttgtatcg tgcatcttgt atcatcatac acgatggctt cttataactg cttctctaaa
     6721 gttaaatagt tttgcaccta gctcttcata agcatggaaa agctcctcaa aaacttcttt
     6781 cttgagagac cctgtttcgt aaagtatctc taagtgaact ttagtttcat cacaggatgc
     6841 aatagcataa gtcaaaaact gaataaattc gttcttgtat ctccttcgcc caaatccctc
     6901 aacaacatta gacactatgg actttgaaga acgccttatt tgaccgcctt cctcatacat
     6961 ctcaaacttt ggtagtttat ctattgtcag tctatgaacc ttaactgcaa actcttttgc
     7021 tggtgaatat acctcgaggt ctttgtatga ttgaggtttt ttcatcgtgc atcctgcatc
     7081 ttgtatcctg catcctcatt actttcattc gtgaccactt gtgtcaatta gcttcgctga
     7141 aatgcccgac cctcaacccc gcattttcgg ggttaaatta ttagtattat ccttctttgc
     7201 ttcttcaacg aaaagcccct caagcgattt tcttaatggg tggagcgaaa ttatatccgc
     7261 cctgcattgg gaagctatct ccaaaacctt agcttttata tcctcatcaa atttgagaac
     7321 aaaaaatccg gttctctttt caacagcaac cttaaggtca gtaaagtaat taactatctt
     7381 ttcatctaca ccttttaaaa gcatctcata atccgtatga attccctcaa agagttttgc
     7441 aagctcacct gtttttacaa cacgccctcc tattataact cccactctat cgcaaagggc
     7501 ttcaacatct gagagtatat gagagctcaa gagaattgtc ttaccaaccc tttttacctc
     7561 caagattaat tcccttatct ccctttgccc catcgggtca aggccactca gcggctcatc
     7621 gagaattaaa aaatcagggt catttataag agcttgggca ataccgattc tctgaagcat
     7681 cccttttgaa tattttctta attgtaacct tcgagcatga gacatcttca ctttctcaag
     7741 gagctcatca accctttttg caagtccctt cccatcaact ccgtgtagtt ctcccatgta
     7801 gtcaagaagt tcttcgcctg ttagataatc atagaaatat ggattttcag gtaagtaacc
     7861 tatatgcttt tttacatcta catctcccat tggcttaccc attatttcaa tccttccgcc
     7921 cgtaagagaa acgaacccaa gtattacttt aaatgttgtg gttttccccg ccccgtttgg
     7981 accaacaaaa ccaaagattt ctcccttatt cacagagaaa gacacatcat ttaaaactcg
     8041 aacctttttt cttaagaacc ccgaagtata gtcctttact agattctcta tctttagcat
     8101 ggtttctaat ttggataatt ttgtaaaatt atatgcctat tataagatct ttacaaatct
     8161 attaatcata aaataacctt gtaagatcac cattccttcg acccctcgac aaagactgtc
     8221 ctaatcgaag ggctcaggga gtctcagcgc aattgatcgc tagcgaataa gttagcatag
     8281 tgccagttcc ccgaacgggc atcatttggg ctgatgaggg catctgcccc taaattttat
     8341 tcctggatgg cggggttaga aaacccagcc tttcgttgtt ggtgaataaa ttaatcatcg
     8401 ccgtggaagg cgggactttc gagtcccgat tgaatcgcgg ccgctaatac aaacgaaatt
     8461 gtcattgcga gcgccagcaa agcaatccca tcattcttac cgtcattgcg aatgaaatga
     8521 agcaacctgt ttgagattcc tcacttcgga atgacacttc gtgttagatt gcttcgtccc
     8581 cttcgacttt gctctagggt tcctcgcaat gacatacaga ggtcgaaaac ctagagcgga
     8641 ggtcaatcac aaaacaaggg gtggcagaaa ccctaccacc ccttgttttc ttagctcaat
     8701 attttaatcg caagctccgg caggaacgcc tccttggcta ccatctcggg tggcttgata
     8761 agttccattt acacctgaag cttgttgaga ggtagtacac cttattaccc catctcccca
     8821 gaccccataa tcccttctac cagtcttcag cacaccactc ggagaggctt cccaaccaaa
     8881 gtcggcttga agaagtgtat caggtaggat agctccagga gggtcactcc aatcagctcc
     8941 atcgagatcc gtatccgtag aatcattagt ccaagcgaca caatagcctg cttttgagtt
     9001 tccgcaatca gctagagcag ccggcgcgcc atcagcctca ctacgagccc ctacaaagct
     9061 gttatctaca agcgcgtctg attcaaggca agtagcacca cctgcaatag tatcatcagg
     9121 acaccgtaga gaaccatcag tagcgacagg gtcttctcct attaaatcag tgaaatccct
     9181 tattccatta tcatctatgt cctgctcaaa gaactctccc tctgcatccc ttagagtctg
     9241 catcgccttt cttgcattcg cctcgtttgc agaaatccta gccttaatta ggttaggaac
     9301 cgcaatagca aggatgatac ctataatcgc cgcaactatg agaagctcaa tcaacgtaaa
     9361 acccttctcg tcctttctac ccctcttaaa ctttaacatc ttgactacct cctttaacga
     9421 gttattgtag gataattata ttgcaattat catgccagtc aaggggaaat aatctgtaat
     9481 ctgataacca tccgaaacaa aaggatttta ttgttttcca caacagggat tacctattcc
     9541 taccctgtta aagtgacaat ttttgtcact ttcggggcta attatgacaa aatttgtcac
     9601 ttcatgagga tatggccggg tatatgcagg taaaccattg agacaaaaag attttatatc
     9661 tttcgataat ctagaattcc atcctcaatc accgctaaag taacaccttt ttggactaat
     9721 catgacaaaa attgtcaatt atacgttcgt tcatcggcaa aattcagtat gttttgcaga
     9781 acctctcagt tggagttttt gaatacaaaa ttagaccact atttccaagt cgctaatttt
     9841 ggttaaatta tattttaatt tgactgaggg ctagaggagt ttttaatttc ttggactgct
     9901 taattcacgt tacacatata tagtgccttg tcatgcccga atgtataaac gttcgattaa
     9961 attggggggc aactacgacc tactctttta tacgaggtta gaaaacctcg gctatcgcag
    10021 tgtaattgtg taaaattatc ctttgatagc tgcgactttc cactcgcaga ttttttttaa
    10081 ttggtagatt tatgactcct gccctgccgc tcctgctttc atacccgact gcagatatcg
    10141 ggtatccatg ttttctttta tggattcccg cttaagatac acgcagatat aacttaataa
    10201 gaataagtgc ggagattcaa ggaagcttaa tttattggtt tttctacaga aaatttccga
    10261 tactcttcac ttaactatag ttataaaatt gtaaattaat aaaataaagg ttaagttcta
    10321 agtgcttata tataatttca aatgaactat aagatacata caatactgag gagaaaaaag
    10381 taatgaataa aaaaatatat tctgtctctt ttctatttat tttttttcta ctctgtttgt
    10441 caatcactgg gtgcggcgac ttgaaggttg cttcatattt taatccttct gacccaaaac
    10501 ttaataaata caagacgctt gaaattgcag attttgagac cgaaataacc gatgttccaa
    10561 acgaagccct gacaaggatt tcagatgagg tagcaaagtt aactgtttct aagaacattg
    10621 gcttccaaaa agtggagcgt ggttcaactg aaggcgaccc cactcagcaa acccttgtcc
    10681 tcgttggtga aataacagga tatgaatcgg gaaaaagctt taaga
//
LOCUS       DATDRL010000336        11775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3816675, whole genome shotgun sequence.
ACCESSION   DATDRL010000336 DATDRL010000000
VERSION     DATDRL010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11775
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3816675"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VI935_11200"
     CDS             <1..447
                     /locus_tag="VI935_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HLE26194.1"
                     /translation="KAQKILEEKYEIATDVWSVTSYKELYREGHEAERWNILHPSELP
                     KIPYVTKCLQDASGVFVAASDYVKAIPDSISRWFPKPLVSLGTDGFGRSDSRRTLRDF
                     FEVDSRFITLATLTALAREKEIKTDVVQQAIKDLDINPAKANPMIS"
     gene            567..1994
                     /locus_tag="VI935_11205"
     CDS             567..1994
                     /locus_tag="VI935_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HLE26195.1"
                     /translation="MTTEFRLPELGENIESGDVVKILVSVGETVIKDQPVMEIETEKA
                     NIEVPSPVNGAVKEIHVKEGEKIKIGQLLLTIDEGAEVEEKKEGVRKQENVEVRREEE
                     KEERKKEKKVEKKEVKAEVHRQEKVKAREEEKVEVRMEAKEEKEKYKVIPKEEGEVVE
                     FSRPAKVASESKPSPELAPAAPSVRRLARELGVDINRVPGSGPGGRVSEEDVKDYARR
                     LLNEAGSVRPAGHTEIAPLPDFTRWGEVERKPMTNVRRKIAEHLSISWTAPHVTQHDK
                     ADITDLEKLRKQIGKKAEAIGGKLTVTAIILRVVASALKVFPQFNTSVDMANNEIIYK
                     KYYHIGVAVDTDRGLLVPVIRDVDKKNIFELSVELSHLSEKARNKKLTLEEMQGGTFT
                     ITNLGGIGGTYFTPILNYPEVAILGISRSSNEPIFNNRQFEPRLILPLSLSYDHRVID
                     GADAIRFLRWIVEALEQPFQLILEG"
     gene            2080..3498
                     /gene="lpdA"
                     /locus_tag="VI935_11210"
     CDS             2080..3498
                     /gene="lpdA"
                     /locus_tag="VI935_11210"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HLE26196.1"
                     /translation="MNEKFQSTQLAVIGGGPGGYPAAFLAADLGLQVTLIDPEPNPGG
                     VCLFRGCIPSKALLHVAKVITEAHHAAHWGVEFSEPTIDLDRLREWKESVVKKLTGGL
                     GQLSKQRKVSYIQGRASLINRQTLKIQNQKTEEFLRFNHAILATGSRPATIPNLSLKS
                     PHILDSTSALDLPDIPKNMLVVGGGYIGLELGIVYAALGTKVSVVEMTSSLMPGVDRD
                     LVSILSKRLEKVFDSIMLDTKVVGMKEEKKGIRVTFEGGGIKEQVFEKVLVSVGRNPN
                     SNNIGLENTKIEIDLQGFIKVDAQRRTTEQMIFAIGDVAGEPMLAHKATHEGRVAAEV
                     IVGRNVTFEPRAIPAVVFTDPEIAWCGLTETQASQEKRPVKIARFPWGASGRATTLDR
                     TDGVTKLIIDPDTERILGVGIVGTCAGELISEVVLAIEMGAVASDLKLCIHPHPTLSE
                     TIMESAEAFFGHSTHIYRPRKG"
     gene            3666..5123
                     /locus_tag="VI935_11215"
     CDS             3666..5123
                     /locus_tag="VI935_11215"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLE26197.1"
                     /translation="MLTEAKNIIGGEYKAGGTGKVKERYNPGTGELVGRFTESTSEDV
                     DDAIRSARRAFEDSHWPRDPKLRVRVLREIHDAIIDDFEYLARIQTSENGKIIRDSRA
                     ELNSAADFFDYYSGLARDLYGRTNLLDQNTMSFVLREPMGVVGIIAPWNAPIVLLARS
                     LAPALAAGNTVVIKPASYTPLTIYALLDKVLERVNDIPKGVINLVLGGGETVGYELVK
                     HEDVDFISFTGSTAAGAVVMREASINIKRLSLELGGKTPNIILEDADFESAIQGAIRG
                     SMLGSAGQICFAGTRVMVHDKIYNKFRDNIIKLIPNLRIGNGLSEDVDVGPVVSDVQL
                     KRVMAYIEEGKKVASLLVGGNQLSDGEWSKGHFVEPTIFDDVPPDSKVAQEEIFGPVV
                     SMIRFKNLDEVAAIANSTKYGLSAAIWTNNINNAFRLARKIKAGVVWINMYGRMFPEA
                     ETGTYKQSGIGGTLRGMEALNTFTEIKDILVNVSG"
     gene            complement(5516..6901)
                     /locus_tag="VI935_11220"
     CDS             complement(5516..6901)
                     /locus_tag="VI935_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLE26198.1"
                     /translation="MGNETMDVAVLKPGVFTWYKDINRKQWLALAAAFLGWTLDAMDL
                     LFYVYALPKIRDTWALTDQHAALLFGVTLGSSALGGVLFGIITDYIGRVRALTYSILM
                     YSVFTGLSALAPNVEIFVVCRFFLGLGMGGEWASGEILVAESWPRQHRGKVIGLVQSG
                     WGFGYILAATLSIAILPHADLEIKLPFLGDIFFHSWRVLFLVGILPAFLVFFVRRHCE
                     EPEIWRTTAEMRSQKKLSEKGQSFTFTQLWRGDLITFTIRAALLTSFCMIAYWGLYTW
                     VPTYLASPVEKGGAGLGLVKGYSWTIAINIGAIIGTITFGNISDRIGRRPTFVGYLVI
                     MAILVPIFGSITEIHEKIGFMGIDTLLLITGPLLGFFGTGFYAGFGAIMAEVFPTRAR
                     GTAQGFCYNFGRGVSALAPYIFALVAGMTMLGHKLGYGFSLATASVFAILAAITVLTF
                     PETKAKQLDIE"
     gene            7171..7554
                     /locus_tag="VI935_11225"
     CDS             7171..7554
                     /locus_tag="VI935_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26199.1"
                     /translation="MLKLFIITLLGAGAIYFLSTNIWTIEDGIAYGKGNPGISGYEHV
                     TAEVTVPAIGPLEFKSVSVDCPKGKRLLGGVFDSNHLRDVGVRATRVMSMGNHDRYIV
                     VFRNMKNKYISEITLKATAICAYVQ"
     gene            complement(7590..8018)
                     /locus_tag="VI935_11230"
     CDS             complement(7590..8018)
                     /locus_tag="VI935_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26200.1"
                     /translation="MKHISIIVLFFVTLFPVSGKAQELPIHDPMVIHPEWYIKVLDWS
                     VYSVWSGVAILHHVTIENTSDIAYKDIKVRALYYSYSITNYGKQISQETGILPVVVPP
                     HSKNTYLEGGATLGASSMGMYGGYLEVLGAVPIIEQAKSK"
     gene            8423..8632
                     /locus_tag="VI935_11235"
     CDS             8423..8632
                     /locus_tag="VI935_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26201.1"
                     /translation="MDPIPIKNGDYEELWRLQKSIQIAHLASDTAKITELGKEMTDKI
                     YKLYPQCVGHKVRFDVSAKRIFIWG"
     gene            complement(8774..9145)
                     /locus_tag="VI935_11240"
     CDS             complement(8774..9145)
                     /locus_tag="VI935_11240"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HLE26202.1"
                     /translation="MEQKKFEELVDKAYQKIPEIFKQKMENIVITVEDYPTQEDLVHL
                     KSRGENTLLGLYRGTPLPQRSVWQVTRLPDKIVLFQKNIEKICRSEKEVEEKAYEVLQ
                     HEIAHYFGLSDREIYELMGPS"
     gene            9343..10836
                     /locus_tag="VI935_11245"
     CDS             9343..10836
                     /locus_tag="VI935_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLE26203.1"
                     /translation="MAKGDVNAFFGLMLDNITNLIILSGLLIGVFNFPKEIVFFKMIP
                     GTAIGILCGNIMYTYIAYKLMKKTGRNDITAMPLGVDTVSLFGYTLGIIAPVYVATKD
                     ADLAWRVGTATIILTGLIKILLSFSSAWIQRLFPQAALLGSIAGVALLLIAFLPAVTL
                     FKSPLVGFISLGIIFLSFYSKRKLVLGLPGALWAVIIGTAIYYGLKVFGVSNGIDFIK
                     EDMSFGIHLPLPALGSFESFRVAFDHISIILPLSIANVIGGVDVTESAEVAGDPYDVR
                     LIIFTEGITTLIGGLFGGVLQTTPYIGHPAYKKMGGRAGYTLATGIFIGLGGILGYIS
                     FIANYLPQEVIVPILIFIGLEITSQAFRRVPEAHAPAVAMCFIPSIANLVVIEVAQLG
                     INDGHLTGEPLLTFTILKMISNGFIVTALIWGATMVSLIDENLKRASLYLFLGAIFSL
                     FGLMHSPYSNGELFLPWSVTDKTPLYLSVSYLILSVLSLCSVKLKKE"
     gene            10992..11324
                     /locus_tag="VI935_11250"
     CDS             10992..11324
                     /locus_tag="VI935_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26204.1"
                     /translation="MDLLISMKAETILKGLELSSNIMTMPTAQSQGYFIAEYREGDSE
                     KINLLKRYVEMLEDKLDKNIFQIELTYHGFKLKKKVDEDIEYMEAWERWVVFSRTLYD
                     ELEIMVDG"
     gene            11361..>11775
                     /locus_tag="VI935_11255"
     CDS             11361..>11775
                     /locus_tag="VI935_11255"
                     /inference="COORDINATES: protein motif:HMM:NF028290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HLE26205.1"
                     /translation="MISPIFHELLVTVINSLGMYLSLWSIIPFICMLLAIAILPLTHS
                     HWWESNKHKAYVSTILGFPVVIYFLIRDYHQLIHTAHEYFSFIILLASLYKISGGILL
                     EGNPEGKPFVNTVFLAVGAVLANLIGTTGASVLLIR"
BASE COUNT         3543 a         2244 c         2582 g         3406 t
ORIGIN      
        1 aaggctcaga agatattaga agagaaatac gaaatcgcta cagatgtatg gagcgtaacc
       61 agctataagg aactttatcg tgaagggcac gaggcagagc gctggaatat actgcacccc
      121 tccgagctac ctaaaatccc atacgtcacc aaatgccttc aggatgcgtc aggggtattt
      181 gtagcggctt cggactacgt taaggcgatt cctgactcta tctctcggtg gtttccaaag
      241 ccccttgtat cgctcgggac cgatggattt ggcagaagtg acagccggcg aacgttgcgt
      301 gactttttcg aggttgattc gagattcatc actctagcta cgctgactgc cctagcgcgg
      361 gagaaggaaa tcaaaaccga tgttgtgcaa caagcaataa aagaccttga tattaaccca
      421 gcaaaggcta atccaatgat ttcataatga aatgaaaatt cttcttagtc acggaggtta
      481 tggaggagac agagaaagat ttttttattt aagaaaaaac tctattagct ctgtgttctc
      541 tgtggctgaa tgaatatgag gagattatga caacggaatt cagacttcct gaactaggtg
      601 aaaatattga atcaggcgat gtggtaaaaa ttttggtatc ggtcggtgag accgttatta
      661 aagaccaacc tgtgatggag atcgaaacag agaaagcaaa tattgaagtg ccttctcccg
      721 taaatggggc tgtcaaggaa atccatgtaa aggagggtga aaagatcaaa ataggacaac
      781 tgctcctgac catagatgaa ggtgctgagg tagaagagaa aaaagaggga gtcaggaaac
      841 aagaaaatgt tgaagttaga agagaggaag agaaagagga aaggaaaaag gaaaagaaag
      901 tagagaaaaa agaagttaaa gccgaagttc acagacagga aaaagtcaag gcgagagaag
      961 aggagaaagt ggaagttaga atggaagcaa aggaagaaaa agagaagtac aaggttatcc
     1021 caaaagagga gggtgaagtc gttgagttta gtcgtccagc taaggttgca agtgagtcta
     1081 agccttctcc agagttagct ccagccgcgc cctcagttcg cagactagca cgtgagcttg
     1141 gggttgatat aaatcgagtg ccgggttccg gtcctggggg acgggtgtcc gaagaagatg
     1201 taaaagatta tgcccgcagg cttctcaatg aggctggcag tgtaagaccc gcaggtcaca
     1261 ccgaaatcgc tcctctgcct gattttacac ggtggggaga agtagagcgt aaacccatga
     1321 ccaacgtacg ccgaaaaata gctgagcact taagcatttc ttggacagct cctcatgtga
     1381 cccagcacga taaggctgat attacagatt tagaaaaact acgaaaacaa attggaaaga
     1441 aagcggaagc aattggcggt aagctgactg taacggccat tattctcaga gtggttgctt
     1501 ctgcgttgaa ggtatttccc caatttaata ctagtgtaga tatggctaac aacgaaatca
     1561 tttacaagaa gtattatcac atcggagtgg ccgtggacac tgatcgtgga ctgttagtac
     1621 cggttattag ggatgtggat aagaaaaata tttttgagtt gtctgttgag cttagtcatt
     1681 tatcagaaaa agcgaggaac aaaaagctca cgcttgaaga gatgcaaggt ggtacattca
     1741 ccattactaa tctgggaggc atcgggggta cttactttac accgatcttg aattatccag
     1801 aagtcgccat tctgggcatt tctcgatcga gcaacgaacc tattttcaat aacagacaat
     1861 ttgagccgcg tttgatcctt ccactttctc tctcttacga tcatcgcgtg atagatggtg
     1921 cggatgccat tcgctttcta aggtggatag ttgaagcgct cgaacaaccg ttccagctca
     1981 ttcttgaggg gtaaaaaatt atttaaccac aagttattat ttgtacacaa gtgaaaaaat
     2041 cgttaactgt tagaccctag acattcaagg agttagaaga tgaatgagaa gtttcaatct
     2101 acacagttgg cagtaatcgg aggcggccct ggtggctatc ctgcggcatt tttagcggct
     2161 gatttaggat tgcaagttac tctaattgat cctgagccta acccaggggg tgtctgtctc
     2221 ttcagaggtt gcattccatc aaaggcatta ctccatgttg ctaaggttat tacagaagca
     2281 caccacgcag ctcattgggg ggttgaattt tccgaaccca cgattgatct tgacaggctt
     2341 agagaatgga aggaatccgt ggttaaaaaa ttgaccggtg gtttagggca gctctccaaa
     2401 caacgcaaag tcagctacat ccagggccga gcaagtttaa tcaatcgtca aaccctcaaa
     2461 attcaaaatc aaaaaacaga agagtttctt agatttaacc atgctatcct tgctacgggt
     2521 tccagacctg ctacaattcc taatctctcg ctcaaaagcc ctcacatact agactctaca
     2581 agcgcgcttg atctgcctga cattcctaaa aacatgcttg tggttggtgg aggctatatc
     2641 ggccttgagc ttggtatcgt gtatgcggct ttgggaacta aagtcagtgt ggttgaaatg
     2701 acttcaagtt tgatgccggg agtagataga gaccttgtgt ccattttatc taagcgttta
     2761 gaaaaggttt ttgattctat catgcttgat actaaggttg tagggatgaa agaagagaaa
     2821 aaagggatcc gtgttacgtt tgagggagga ggtatcaagg aacaggtatt tgagaaggtt
     2881 ctagtttctg taggccgaaa tccaaattcg aataatatcg gattagagaa tacaaaaata
     2941 gagattgact tacaaggttt tataaaggtg gatgcgcaac gtagaacgac agagcaaatg
     3001 atttttgcaa ttggagacgt cgcaggtgaa ccaatgcttg ctcataaggc tactcatgaa
     3061 ggtcgagttg cagcagaggt cattgtagga cgtaacgtta catttgagcc tcgagcgata
     3121 cctgctgtag ttttcacaga tccggaaatt gcatggtgtg gcctcactga gacacaggca
     3181 agtcaggaaa agcgccctgt taagatagca cgcttcccat ggggggcttc tggtagagcg
     3241 acgacattag atcgaactga tggggtgaca aagcttatca tagaccctga tactgaaaga
     3301 atattgggtg tagggattgt tggtacttgt gctggcgaac taatatcaga agttgtgctt
     3361 gctattgaga tgggggcagt tgcttcagac ctaaagcttt gtattcatcc acatcctact
     3421 ctatctgaaa caattatgga atcggctgag gccttctttg gtcatagcac ccacatatat
     3481 agaccgagaa aaggttgagt acaggagata aaaggttata aactaggcaa aaaatataac
     3541 ctaagataga aaaaagagat tcgtatacat taagaactcc atttttgttt gactttgttt
     3601 aaattagtaa tgtattttat tttccttaat cgcgataatt gaatttccct tagggggatc
     3661 attgaatgtt aactgaggct aagaatatta ttggtgggga atataaagca ggcggtacag
     3721 gcaaagtaaa ggagaggtac aatccgggaa ccggtgagtt ggtaggaagg tttacggagt
     3781 caacaagcga agacgttgac gatgcaatcc ggtcagctag aagggcattt gaggactcac
     3841 actggcccag ggacccgaag ctccgggtcc gagtattaag agagatacac gacgccataa
     3901 ttgatgactt tgagtatcta gctaggatac agacctcgga gaacggtaag ataatacgcg
     3961 actcgagagc agagcttaat tcggcggctg atttttttga ctattactct ggattggcgc
     4021 gcgaccttta tggtagaaca aatcttctag accaaaacac aatgagcttt gtccttaggg
     4081 agccaatggg tgtagtggga attatagctc catggaacgc tcccatagtt ctccttgcaa
     4141 gatcacttgc gcctgctcta gctgcaggca ataccgttgt gattaagccc gcgagttata
     4201 ccccattaac catctatgca ttactcgata aagttttgga aagggtaaat gacataccaa
     4261 agggcgtgat aaatcttgtg ttgggtgggg gcgagactgt tggttatgaa ctcgtcaagc
     4321 acgaagatgt agatttcatc agctttacag gaagcacagc ggccggagcc gtagtcatgc
     4381 gtgaagcaag tattaatata aagcggttat ccttagagct cggtgggaag acgccaaata
     4441 taatccttga agatgccgat ttcgagtctg ccatccaagg agctataagg ggctcaatgc
     4501 ttgggagcgc cggtcagata tgctttgcag ggactcgcgt gatggttcat gacaagatat
     4561 acaataaatt cagagataac ataattaaac tcatccctaa tcttaggata ggcaatggac
     4621 ttagcgagga tgtggatgtt ggtccagtgg tttctgatgt gcagttaaaa cgcgtcatgg
     4681 cctatattga agagggtaag aaggttgctt cgttactggt gggcggcaac caattatctg
     4741 atggggaatg gtcaaagggt cacttcgtgg agcctacaat cttcgacgat gtgccgccag
     4801 attctaaggt agcccaggaa gagatcttcg gtcccgtagt ctcgatgatt aggttcaaaa
     4861 atctggatga ggtagcagca atagctaaca gcacaaaata tggcttatca gcggctattt
     4921 ggaccaataa catcaataat gcctttagac tggcaagaaa gatcaaagca ggagtagttt
     4981 ggataaacat gtacggcagg atgttcccag aggctgagac cggaacctac aagcaaagcg
     5041 gaataggagg aacgcttagg ggaatggaag ccttaaatac ctttacagaa atcaaggata
     5101 tattggttaa tgtcagtggc taatccgttg ataaattaat gtagcttcct cttttctaaa
     5161 ttgacttcca tctctagaag aaagaacgga acaggaatgt tataagccta ttatggttga
     5221 tgggattaat aaacctaagg gcgaaggctg gggtttataa cccttccaga ccggcgagac
     5281 tagaaagtct cgtctatcgt tcccaaaatc tattgtgatt agtgagacta atgatccttg
     5341 aatatttaaa tataccgata ggcgggacat tcctgtcccg ttggcgggat tggagaatcc
     5401 tgactattga agggtatggt taatcaatta cacggtggta gccccgattt cgtagaatgg
     5461 ataggcgggg ttttctaacc ccatactcgg ctttgcaaag agaggcttcg ttttactatt
     5521 caatatctaa ttgctttgcc tttgtctcag gaaatgtaag aaccgtaata gctgccagta
     5581 ttgcaaagac agaagccgtc gcaagtgaaa aaccgtatcc aagcttgtgt cctagcatcg
     5641 tcattccagc aacaagcgca aatatgtaag gtgctaaagc cgaaacccct cttccaaagt
     5701 tatagcagaa tccctgtgct gtgccgcgtg ctctagtggg gaaaacttct gccataatag
     5761 ccccaaagcc agcatagaat ccagtaccaa aaaagccgag gagaggcccc gtgattaata
     5821 gaagggtatc aatccccatg aaccctattt tctcatgtat ctctgtaatg gaaccaaaga
     5881 ttgggaccaa gatcgccatt ataacaagat aaccgacaaa agttggtctt cttccaattc
     5941 tatcgctaat attaccaaat gtaatagtcc cgataatcgc ccctatgttt attgcgattg
     6001 tccatgaata accctttact aatccaagac ctgcaccgcc tttttcaact gggcttgcga
     6061 gatatgtagg cacccaagtg tataaccccc agtaggcaat catacagaag ctcgtaagca
     6121 atgctgccct gattgtaaag gttatgagat cacctctcca aagctgagta aaagtaaacg
     6181 attgaccttt ttcgcttaat ttcttctgac tcctcatttc agccgtagtt ctccaaattt
     6241 caggctcttc gcaatgcctt ctgacaaaaa acacaagaaa agcaggaagg attccgacaa
     6301 ggaaaagaac cctccaacta tggaaaaaga tatctccaag aaatggaagt ttaatctcta
     6361 aatcggcatg agggaggatt gcaatagaaa gagttgccgc cagaatataa ccgaaacccc
     6421 aaccgctttg cactaacccg atgactttgc ccctgtgctg tcttggccag ctttcagcaa
     6481 caagaatctc tccagatgcc cactcaccac ccatgccaag gccaaggaag aaacgacata
     6541 ctacaaagat ttctacatta ggagcaagtg cacttagccc tgtaaataca gaatacataa
     6601 gaatcgaata agtaagcgct cttactctcc caatataatc tgtaatgata ccaaaaagca
     6661 ctccacctaa ggcggaagaa ccaagtgtaa caccgaaaag cagagcagcg tgttgatcag
     6721 ttaacgccca agtgtcgcgt atcttaggta gggcatagac ataaaaaagt agatccatcg
     6781 catccaacgt ccagcctaga aatgctgcgg caagggcgag ccattgtttt cgatttatgt
     6841 ctttgtacca agtaaataca cctggtttta gtactgcaac atccattgtt tcgttaccca
     6901 tttattacgc tccagttgta ttttttctat tctagtaaca gttaaataat tggatttgat
     6961 aacccggtcc atgtctccaa tctcttcatt ttcataatcc acatgccgca attgattaaa
     7021 ttgagagatt atcttaataa aaaaaacatt cgaactcaat aaaagaaaac tacactgttg
     7081 aaaaaaggta atttaaatta attgtctata tcagttataa tgagcataat atttttgtct
     7141 tttagaatct acaacaggag gacatatcaa atgctaaaat tatttataat aactttgcta
     7201 ggagcagggg ctatttactt tctatcaact aacatatgga ctattgaaga tggaatagct
     7261 tatggaaaag gtaatccagg tatttcaggt tatgagcatg ttactgccga agttacggtt
     7321 ccagcaattg gaccgcttga atttaaatct gtttcagtag attgtcctaa aggtaaaaga
     7381 ttgctaggcg gtgtttttga tagtaaccat ctacgtgatg ttggcgtgcg tgccacaaga
     7441 gtgatgtcga tgggtaacca tgatcgttat atagtagttt ttaggaatat gaaaaataag
     7501 tatatatctg aaataactct aaaagctaca gccatatgtg cgtacgtaca gtagctataa
     7561 ataatgtccg ggaaaaatat agatgtatat tattttgatt tagcctgttc tatgattggt
     7621 actgccccta agacctctag ataaccgcca tacataccca tcgaacttgc tcctagggta
     7681 gctccacctt caagataagt atttttgctg tgaggtggta caaccacagg taatattcct
     7741 gtttcttgac ttatctgttt tccgtagttt gtaattgaat aagagtaata aagcgcccgt
     7801 accttgatgt ccttatatgc gatgtcgctt gtgttctcaa tagttacatg atggagaata
     7861 gccacaccgc tccagactga ataaacagac cagtccaaaa ctttaatata ccattctggg
     7921 tgtataacca ttggatcatg tattggtagt tcttgggctt ttccacttac tggaaatagg
     7981 gttacaaaaa ataaaactat aatagatata tgtttcattt tttttatttt aaactagaag
     8041 taacaacatt tccatgagag taataattac ttatcattat ttaacttgag aattccctgt
     8101 agcttgctac agggtttcca caatgctgta ttgtcattgc gaacacattc acttctctca
     8161 gtgtaaactc cgtgaagcga ttctatgctt gcttgtcatt gcgagcgtta gcgaagcaat
     8221 ctcgtatttt ttggattgct tcgtcccctt cgacttcgct cagggctcct cgcaatgaca
     8281 gttgcggcgt ttaatattaa gaccataccc tgcagcaagc tgcagtgtta tcgatttaaa
     8341 atcaatcata tcctaggctt cgtaaacatt gactttcaca tattccattt aacataatat
     8401 ataagatgaa ggaggttcta atatggatcc aatacctatt aaaaatggag attatgaaga
     8461 gttgtggcgt ttacaaaaat caatacaaat cgcccatttg gcaagtgaca ctgctaaaat
     8521 aactgagtta ggaaaagaga tgactgataa aatatataaa ttgtatcctc aatgtgttgg
     8581 tcataaggta cgctttgacg tgtctgctaa gagaattttt atttggggat gaaagaataa
     8641 aaatagtgtc tgttctattt tttctatgtt accttgaaaa agattgtagg ggttgatgcc
     8701 ttcatcagcc cctcgatgaa ttcgtcgggg gctcgttcgg ggaacgagcc ctacaattaa
     8761 atcccttgta gctttaactc ggacccataa gttcataaat ctctctgtca gagagtccaa
     8821 agtagtgagc gatctcatgc tgaagaactt cgtaagcctt ctcttctact tctttttcac
     8881 ttctacaaat tttttcaatg tttttctgaa atagaacgat tttatcaggc aaacgtgtaa
     8941 cctgccatac gcttctttgt ggaagagggg ttcctctata gagtccaaga agtgtattct
     9001 cacccctact tttaagatgc actaaatcct cttgggttgg ataatcttca acagtgatta
     9061 ctatattttc catcttctgc ttaaatattt ctggaatttt ttgataggct ttatctacaa
     9121 gttcttcaaa ttttttttgt tccaatttac ttccttagtt taagtgcacg atttatagag
     9181 agatgataat gatttaagga ataaattcaa ataaatatcg acattttttg cgctgaaagt
     9241 cattttttaa atacaactct tagaataata aattagactg tacacttgat tttaagcttt
     9301 cataaagcta tatctattac cccaatgaaa ccttaatctt gggtggcaaa gggtgatgta
     9361 aatgcctttt tcgggcttat gcttgataac attactaatc ttataattct ttcaggcctt
     9421 cttatagggg tttttaattt tccaaaagag atagtttttt tcaaaatgat tcctggaaca
     9481 gctattggga ttctatgtgg aaatataatg tatacataca tcgcctataa gctcatgaag
     9541 aaaactggga gaaatgatat tacggctatg ccgcttggag ttgatacagt atctcttttt
     9601 ggatatacgc ttggcataat agcaccggtt tacgtagcta caaaggatgc agaccttgcc
     9661 tggcgtgtag gcacagcgac gattattcta acggggttaa ttaagattct gctttccttc
     9721 tcaagcgcat ggatacagcg tctatttcca caagcggctc tcctagggtc tatagctgga
     9781 gttgcgctac ttcttatagc ctttctaccg gctgtaacat tatttaaatc accgttagtc
     9841 ggatttatct ccctaggaat aatatttctc agcttttatt cgaaaagaaa gcttgtcttg
     9901 ggtctcccag gcgctctctg ggccgtcata atcggaactg ctatttatta tgggttaaaa
     9961 gtttttggtg tctctaacgg gattgatttt attaaagaag acatgtcatt tggcattcat
    10021 cttccgctac cagctctcgg gtcatttgaa tccttccgcg tagcatttga tcatatctct
    10081 attatccttc ctctcagtat agcaaacgtt attggtggag ttgatgttac agagagcgct
    10141 gaggttgcag gtgatcctta tgatgtaagg cttattattt ttactgaagg aattactact
    10201 cttattggtg ggctttttgg aggggtgctt caaacaactc cttatatcgg tcatcctgcc
    10261 tataagaaaa tggggggtag ggcaggctac acattagcta ctggcatatt tatcggtctt
    10321 ggaggaatcc ttggttatat ttctttcatt gccaactacc taccacagga ggtaatcgta
    10381 ccaatattga tttttatcgg tcttgaaatt acatcacaag catttcgtcg tgttccagag
    10441 gctcacgctc cagcggtagc gatgtgtttt attccatcaa ttgcaaattt agtagttatt
    10501 gaagtagcac aacttggcat taatgatggt catcttactg gggaacccct tttaacattc
    10561 actatactaa aaatgatttc aaatggtttt attgtaactg cgcttatatg gggtgctaca
    10621 atggtaagcc taatagacga aaatctgaaa cgcgcatcgc tttatctctt tcttggagct
    10681 atattttctc tttttggcct tatgcactct ccatactcta atggtgaact ttttcttcct
    10741 tggagtgtga cggataaaac tcctctttac ctctcggttt cttatctaat tctctcagta
    10801 ctttcactat gctcagtgaa gctaaaaaag gaatagctaa taaagagtgt tgacatattt
    10861 agtattctgg tcttaaacta aacttcctaa agcttactta tataaatgaa tgccactaaa
    10921 acaccaagac acaaagtttt tttattttat tacttaggga cttcgtgtct tagtggcaaa
    10981 gaaaggttag gatggacctt ctaatctcta tgaaggctga gacaattcta aagggactag
    11041 aacttagttc aaatataatg acgatgccta ccgcccagtc tcaaggatat tttattgctg
    11101 aatatagaga aggggattca gaaaagatta atctccttaa acgctatgta gagatgcttg
    11161 aagataaact cgataaaaat atatttcaga tagaactcac ctatcatgga tttaaactta
    11221 agaaaaaggt tgatgaggat atcgaataca tggaggcttg ggaaagatgg gtagtatttt
    11281 caaggacgtt atatgatgaa ctcgagatta tggtagacgg ttgatgaacc tggttaagct
    11341 cacatttact ttcacatccg atgataagcc caatattcca tgaactctta gtaactgtaa
    11401 taaatagttt aggtatgtat ctttcccttt ggagcattat accatttatt tgtatgctgc
    11461 ttgcaattgc gattctccct cttactcata gtcactggtg ggaaagtaat aaacataagg
    11521 cttatgttag cacaatttta gggtttcctg ttgtaattta ttttcttata agagattatc
    11581 atcaactcat tcataccgcg catgaatatt tttctttcat aattcttctt gcatctctct
    11641 ataaaatttc tggtgggatt ttacttgaag ggaaccctga gggaaagccc tttgtaaata
    11701 ctgtatttct tgctgtaggt gctgtgcttg cgaacctaat tggaacgacc ggagccagcg
    11761 tgcttcttat aagac
//
LOCUS       DATDRL010000337         2767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2968562, whole genome shotgun sequence.
ACCESSION   DATDRL010000337 DATDRL010000000
VERSION     DATDRL010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2968562"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(536..1138)
                     /locus_tag="VI935_11260"
     CDS             complement(536..1138)
                     /locus_tag="VI935_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26206.1"
                     /translation="MKKVRTVALSLMVVFSFVILLAGTGIAQMQDIEGNIVCIEVDEK
                     GNVNAMEEYTECDGVLMMVGKDGKLYAVSGTEEQMKMVAKSPKKKASGQVGGSQRAWV
                     IYATPTDVQKGTAQTVTGNIVCLLPSYEKGNVAPVVSTGPCNEAVPHAHVVTTASGQV
                     YILSGSEDAISSIEKSSQRTNVTLSGKVTGNQGAWILYVE"
     gene            complement(1255..2052)
                     /gene="fetB"
                     /locus_tag="VI935_11265"
     CDS             complement(1255..2052)
                     /gene="fetB"
                     /locus_tag="VI935_11265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HLE26207.1"
                     /translation="MEGLNYTLIDISYIDLIISLGLIIVAAGFSRWQGMYLEGDLIVG
                     AIRTFIQLLAVGYILKYIFAFDRWYLIIFIILIMIIIASLTVTQRQKKRTKELPFIVG
                     FAMTVSSGITVFVVTQILLQIKPWYEPHYLIPLAGMVIGNSMNGSTLAIERIDSEIES
                     RKMEIEAYLALGATARQAAGAPIRAAMKAAMVPTINSMMVVGIVALPGMMTGQILSGT
                     SPLIAVKYQIIIMYMIALSVAISAYLLTNIRFNRYFTSDHRLKEESL"
     gene            complement(2069..>2767)
                     /locus_tag="VI935_11270"
     CDS             complement(2069..>2767)
                     /locus_tag="VI935_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLE26208.1"
                     /translation="MNKPKIELHKIGKNKNGITILKDINLKFSSGKRHILIGPSGAGK
                     TTLLRLLNRMDEPTFGEILYDGKNFKDIPVLELRRRIGMVFQIPIIFDGSVKDNLNVP
                     YRLRVISNPVKQDELIKIIELSGLQSSFLERKASELSVGEKQRLTVARALINKPDVLL
                     LDEPTSALDHASGIKLLESIKELNEALGLTIIMVTHQLEYAILLGGRIIHLVNGSIVR
                     EQESEDFFLNSEIR"
BASE COUNT          842 a          548 c          482 g          895 t
ORIGIN      
        1 ctcaggaaga ttggagtaca gaagagaaaa aaacaaaatt accacaaaaa ctatgataaa
       61 aatcaccaag atttcgagta tattttccgt gaggcagcga attaaactct cgaatagatt
      121 tttatatgag gcaatagtta catatgaaaa ccgtggaagg ctagagaatt tagtttaggg
      181 gagtcatcat atatttcgtc gtactgctat ccaaatagat gagtctatgt tgggtttgga
      241 tacaagtcta tatacttcca gcttaaggct gatcgaagat actgaataga attatatcct
      301 tagtttttta aaagagacga attgaataat tgatggtaaa ccaggtgatg cgcttattcc
      361 aggtatgaat tctggcacaa ttagatctcg aatgaaaaaa cttaatatca tgaaaaaaat
      421 taaagattta tctgcctttt aaagcaaata aatctcttaa tgaaagttaa tgaagaaaag
      481 agggctttag aaaacactaa aaccctctcg atattgttag cgattatata gttagttatt
      541 ctacatacag gatccaagct ccctgattcc ctgtaacctt accgctgagt gttacatttg
      601 tcctctgaga gctcttttct atactactaa ttgcgtcctc agaaccactt aatatataaa
      661 cctgtccgct cgcagtagtt acaacatgtg catgcggcac agcttcattg caaggtccag
      721 ttgatacaac tggcgcaacg ttaccctttt cataactcgg tagtaagcaa actatgttac
      781 ctgttacggt ttgggctgta cccttctgga catctgttgg cgttgcgtag ataacccagg
      841 ctctctggct acctccaact tgtccactgg cctttttctt agggctcttt gccaccatct
      901 tcatttgttc ctctgtacca gagacggcat ataacttacc atctttacct accatcatga
      961 gcaccccatc acattcggta tattcttcca tagcattgac gttacctttc tcatcaacct
     1021 caatgcaaac aatattaccc tcaatgtcct gcatttgtgc aatgcctgtg ccagctagta
     1081 aaattacaaa agaaaaaacc accattagag atagagctac tgtccttacc ttcttcattt
     1141 tgtttacctc ctccttggtt ttaaatcaaa tttagtatat aattatacca aaaaaaaaat
     1201 tggatgcaat attaattaac tcagaaatta actcagaaat aatacaatgc ctactcataa
     1261 gctctcttct tttaggcgat ggtcagaggt gaagtatcta ttaaatctaa tgtttgtaag
     1321 gagatatgcc gaaatagcta cagagagagc tatcatgtac atgataataa tttggtactt
     1381 aacagcgatt aaaggtgaag ttccagatag tatttgaccc gtcatcatgc caggaagtgc
     1441 tacaatacct acaaccatca tggaattgat ggttggaacc attgctgcct tcatagcagc
     1501 tcttataggg gcccctgcag cttgtcttgc agtcgctcct agtgccaaat aggcctcaat
     1561 ttccattttt ctagattcta tctctgaatc aattctttca attgcaagtg ttgagccatt
     1621 catgctgtta cctattacca ttcctgcaag gggtataaga taatgcggct cataccaggg
     1681 tttaatttga agaagtattt gggtaacaac aaaaacagtt attcctgagc taacagtcat
     1741 tgcgaatccg actataaatg ggagttcttt tgttcttttc ttctgccttt gtgtcacagt
     1801 tagagatgct attattatca taataagaat tatgaatata attagatacc aacgatcgaa
     1861 agcgaatatg tatttaagta tatatcccac tgcaagcaac tgaatgaagg ttctgatagc
     1921 tcctactatt aaatcacctt ctagatacat accctgccat cttgagaacc ctgcagctac
     1981 tataatcaaa ccaaggctta ttattaaatc tatgtagcta atgtcaatta gggtgtaatt
     2041 gagtccctcc atatgttttc tcctacgttt aacggatctc cgaatttaag aaaaaatctt
     2101 cgctttcctg ttctctaact atagatccat ttactagatg aataattcgg ccgcctaaaa
     2161 gaattgcata ttcaagctga tgagtaacca ttataattgt aagccctaag gcttcattta
     2221 actccttgat cgattcaaga agttttatcc ctgaagcgtg gtctagtgcg gaggttggtt
     2281 catctagaag aagcacatcg ggtttattta tgagggctct tgctacagta agacgttgtt
     2341 tttcaccaac tgaaagctca gaagcttttc gttcaagaaa actgctttga agtccagata
     2401 attctataat ctttatcaat tcatcctgtt ttacagggtt tgatataact cgcagacgat
     2461 agggaacatt taggttgtct ttgacgcttc catcaaatat tatagggatc tggaaaacca
     2521 ttccgattct acgtcttagt tccaagactg gaatatcttt aaaattcttc ccatcatata
     2581 aaatctcacc aaaggttggt tcgtccatgc gatttaaaag cctaagaagg gttgtctttc
     2641 ctgcaccaga gggtcctatg agaatgtgtc tttttcctga agaaaattta aggtttatat
     2701 cttttaaaat tgtaattcca tttttgtttt ttcctatttt gtgaagctcg atcttcggtt
     2761 tgttcat
//
LOCUS       DATDRL010000338         6780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1183180, whole genome shotgun sequence.
ACCESSION   DATDRL010000338 DATDRL010000000
VERSION     DATDRL010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183180"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VI935_11275"
     CDS             complement(<1..216)
                     /locus_tag="VI935_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HLE26209.1"
                     /translation="MFPQTVGDVLKLMKEKNIEVVDLRFLDFLGTWQHFSAPPHELDE
                     DAFEEGLGFDGSSIRGWQSIHASDMLVI"
     gene            complement(309..647)
                     /locus_tag="VI935_11280"
     CDS             complement(309..647)
                     /locus_tag="VI935_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HLE26210.1"
                     /translation="MKKIEAIIKPFKLDDVKESLKEVGVQGLTVTEIKGFGRQKGHTE
                     LYRGAEYVVDFLPKIKLEIIVSDDMVTRVVDAIMDSARTGKIGDGKIFILPMEEVIRI
                     RTGERGEDAL"
     gene            complement(943..2670)
                     /locus_tag="VI935_11285"
     CDS             complement(943..2670)
                     /locus_tag="VI935_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLE26211.1"
                     /translation="MQLLFKPRDSKYLGQGTLPFGTFDDTSEWLPITKCLEKGALRFP
                     DKPMFKIADKDGNIVETYSYRETNEWANRISNGLRQRFGVKKGDKVGMYMINCSEYVV
                     SIIAIHKAGAVQVPINKDEKGERLAYVINYSDMVSLIIDPDSIPFIQEIAKDLTNLKT
                     IFVTRDPKDVPDSINGIKALSFTVFNDFSSDNPVVDVTIADPERCMFTSGTTGMPKGV
                     ARNHGGVILTVRAYLQQQGVRSGDTLMSVLSLGHANAQVMCLFSAMTAGATAVFFPRF
                     SASSFWKWASECGATCVNMLGAVSEYLWAAPPSEWDKKHKVRIMLGSPAPRNLAEFQE
                     RFGVRVIDGYGSTEMGMVLWKDPEDHRPGSSGYSMEGYHVELRDPQDTNKALRPFWDP
                     YENPTPPEEAKGLLYIKPLIPHTTLNEYFKDARRTIEAFDDDGFFNSDDLFARGIDGR
                     YYFVGRFSRIRVSGENVDPIAVQDLAMRYPAIQEAIAVGIRLPNVSDDEIKLNITLKP
                     GETFDSVEFSKWMAELVMVAMVPRFIEIYPEGFPVTATQKIKMGELKEISDRTWDRAK
                     TGLKFSARK"
     gene            3116..3793
                     /gene="rnc"
                     /locus_tag="VI935_11290"
     CDS             3116..3793
                     /gene="rnc"
                     /locus_tag="VI935_11290"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HLE26212.1"
                     /translation="MIKKLGYRFKNPGLLKIALTHSSFANETSVESNERLEFLGDAIL
                     GCVVARFLYDHYPQASEGILSKMRSAIVSRSNFARFARELRMDKELLLGKGEELTGGR
                     ERESNLAGVFEAVIGAVYLEAGYRKAFQITSRLIKSCLEKREIFMDYKTTLQELVQKK
                     SGSTPRYKVVLEEGPPHSKYFHVEVKVARRVLGKGSGRSKKEAEQAAAKKGLENLETS
                     GQAESSR"
     gene            complement(3772..5217)
                     /locus_tag="VI935_11295"
     CDS             complement(3772..5217)
                     /locus_tag="VI935_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLE26213.1"
                     /translation="MKQRVLVVDDEESIRWVLGKCLEKAGYTVEFGENGSEAIQKATL
                     DNFSLIILDIKMPDLNGLEVLEELRVKGIDIPVIIITAQNTVKNAIEAMKRGAYDYIA
                     KPFNLDEVKLTVERAIENHENSKRLENLRATLREGKSSEPHQGIVGKSPAMLKIYKTI
                     GRVAEKDLTVLITGESGTGKELVARAIHFNSQRRDERFVPVNIAALPKELLESELFGY
                     EKGAFTGATIRKQGRFEDADRGTLLLDEIGDMTPELQTKLLRVLEEKQFYRLGAEKPV
                     EVDVRVIASTNKNLEKEVEKGSFRRDLFYRLNAITIELPSLRERKEDIPVLSEHFLEK
                     YGKELGVGKRIISDEAKEILLGYNWYGNVRELENTIKRVLVLSSDVMITSQVLLEAAP
                     YLEGIKKKEEDSLNKLITTRLLNMIDLTQEPPPNGIYDLIMRSVEKPLIEAVLKIAKG
                     NKKKAAKILGINRNTLAKKLDDFGIHLEDSA"
     gene            complement(5362..6459)
                     /locus_tag="VI935_11300"
     CDS             complement(5362..6459)
                     /locus_tag="VI935_11300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLE26214.1"
                     /translation="MNRATKTREDFFQDILDALPDGIVALDKSFNIISLNHAAETIFK
                     ISREKARGMSSSTVLPEEMEAIAEKSIQDERTVFSDEINPVLKGGEKVSIQVVSSPLF
                     SDGSNALGVILQIKDLSSTKFLSEKNLQQSSASTLEGLLIGLTHELKNPLSGIKGAAQ
                     LLLQEKTIDVTAKCADIITKEVDRLCTLLDRLGQIEPLPKEVFEPVDIHDIISEIIFL
                     ESKSRDGKRVKFVENFDITLPPVLGNKDSLKQVFLNIIKNSIQAIPNRGSIEITTRWI
                     TDYKLKGEKAILVEIRDTGSGISKEILQKIYKPFYTTKKEGSGLGLFIAYQIIAKHGG
                     AIFVESELGKGTVFKVYLPIYRNEEIWNSGV"
     gene            complement(6459..>6780)
                     /locus_tag="VI935_11305"
     CDS             complement(6459..>6780)
                     /locus_tag="VI935_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HLE26215.1"
                     /translation="LPKSLQGTTSIPSGLVPISEITTKYYLRFQVEDRPGVLGQIAGS
                     LGRNNISIESVIQKGRHIGGEVPVVIMTHEAKERDLMSAIEEIDRFPVIKSKSVFIRI
                     EDLS"
BASE COUNT         1820 a         1557 c         1206 g         2197 t
ORIGIN      
        1 aattacgagc atgtcacttg cgtgaatgga ctgccatcca cgaatacttg agccgtcaaa
       61 acctaaaccc tcttcaaatg catcttcgtc aagctcatgt ggaggggcgc tgaagtgctg
      121 ccacgttcca agaaaatcaa gaaatcttaa gtccacaact tcaatatttt tttctttcat
      181 cagttttaat acatctccca cagtttgtgg aaacatatgg tttgtacctc ccttattttt
      241 tttacttgga ttgccctttt ttgtaaccat atcagctatg tcctcctcgt ataggcgagt
      301 ttttcatttt atagcgcgtc ctcgccgcgc tcgccggttc ttattcttat tacctcttcc
      361 ataggaagaa taaatatctt cccatcccca attttccccg ttcgagcact gtccatgata
      421 gcatctacaa cccttgttac catatcatca gagactatta tctcaagctt tatctttggc
      481 aaaaaatcaa ctacatactc tgcacctctg taaagctctg tatgtccctt ttgccttccg
      541 aaccctttga tttctgtcac ggttaatccc tgaacaccta cttcttttaa cgattctttt
      601 acgtcatcga gtttaaaggg ttttattatg gcttctatct ttttcataaa attaacctcc
      661 tcatctcctg agtatgcaaa caacatacca cctttcaaaa tccttttcaa tcattaaaac
      721 aagcctattt gccgaatttt tgagcctggt agtgcctaac ttctaggcac cgtgtcttaa
      781 aaaatagaca cgcgtttgtt ggtctattag tggatttatg aggtattaac catggagttc
      841 aaagactacg gtgagaaaaa atatatgggc gttaagttat agccacctaa attttttccc
      901 ataatcagga caggcaaaga ggttttcctt tgcgtcacac atttatttcc ttgcgctaaa
      961 cttaagtcct gtctttgctc tatcccatgt cctatcgctt atctccttta gctcccccat
     1021 ctttatcttc tgtgtcgctg tcactggaaa tccttctgga tatatctcaa taaacctcgg
     1081 caccatcgct accatcacta gctccgccat ccacttacta aactccaccg aatcaaatgt
     1141 ctccccgggc tttagtgtaa tatttaactt tatctcatca tccgatacat tcggcagcct
     1201 tatccctacc gctatagcct cctgtatcgc tggatacctc atcgctaaat cctgcaccgc
     1261 tatagggtct acgttctctc ccgataccct tatcctgcta aaccttccaa caaagtaata
     1321 cctcccgtct atccctctgg caaacaggtc atctgagtta aaaaatccat catcgtcaaa
     1381 cgcctctatt gtgcgtcttg catccttgaa atactcattt aacgtcgtgt gcggtatcaa
     1441 aggttttata tatagtaacc cttttgcttc ctctggcggt gtgggattct cataagggtc
     1501 ccagaagggg cggagagctt tatttgtatc ttgagggtct cgtaactcta cgtgataccc
     1561 ttccattgaa tagcctgagg agcccgggcg gtggtcttcc gggtctttcc acagaaccat
     1621 gcccatctct gttgatccat acccgtctat aacacgaaca ccaaacctct cttggaattc
     1681 tgctaggttt ctaggcgctg gggatccaag cataatgcgg accttatgct tcttatccca
     1741 ctcacttggt ggtgcggccc aaaggtactc tgaaacggca ccaagcatat ttacgcaagt
     1801 agcaccacac tctgaagccc acttccagaa gcttgaggct gaaaaacgtg gaaagaaaac
     1861 tgcggttgcc cctgccgtca ttgcgctaaa taaacacatc acctgagcat ttgcgtggcc
     1921 tagagaaagc acgctcataa gggtatcacc gctacgcacc ccctgctgct ggagataggc
     1981 ccttactgtt agaattaccc ctccgtgatt tctagcgacg cctttgggca tcccagttgt
     2041 cccagatgta aacatacatc gttctggatc tgcaattgtc acatctacca caggattgtc
     2101 actagaaaaa tcattaaata cagtaaagga tagggctttt attccgttaa tgctatcagg
     2161 gacatccttt gggtctctag taacaaatat ggtctttaga ttcgttaaat ccttggcaat
     2221 ttcttgaatg aaagggatgc tatctgggtc aattattaac gacaccatat cggagtaatt
     2281 aattacgtaa gctagtcgtt cgcctttttc atctttgtta attggcacct gtacagcacc
     2341 agccttgtga atggctatga tagatacgac gtattctgag cagttgatca tatacatccc
     2401 aaccttatcg ccctttttta cgccaaacct ttgccttagg ccattagata tcctgttagc
     2461 ccattcattt gtctccctat aggaatatgt ttcaactatg ttgccatcct tgtctgcaat
     2521 tttgaacatg ggtttatcag ggaacctaag agcgcccttt tctaggcatt tagtaatagg
     2581 aagccactct gaggtgtcat caaatgtgcc aaagggtaag gttccctgac ccaggtattt
     2641 tgagtctcgg ggcttaaata aaagttgcat agccatatcc tccctcttta aataaacgat
     2701 aacaaatagt ctttcactaa ttaaaagatt gttataacag gtgttcgaaa gatcttattc
     2761 atatcttata tcaaattgac attttttgtc aagaaaattt cttcatcagt tcactttctc
     2821 aactcatacc tctttgccca atcatgtaaa taggtatatg ttaaacgagg tgatcttcta
     2881 accaactctt ctaagtaatt ccatattagt aatagttatt gggaaggttt taagcaatat
     2941 cagtatgtaa tcctataact cttaagtaaa ttcacctttt atggttgtga agtctgccta
     3001 gatctcgggg tggagcttaa aatgacacac agagttttca gggataacca ctattatcaa
     3061 gatctaaacc gattattgat tttcctcaac cttatgatag gtttttacat ccgtaatgat
     3121 aaagaagctt ggttataggt ttaaaaaccc agggcttcta aaaatagctc taacgcatag
     3181 ttcattcgcc aacgaaacct cggtagagag taacgaacgg cttgagtttc ttggagacgc
     3241 aatccttggt tgtgtcgttg cacgatttct ctatgatcac tatcctcaag catctgaagg
     3301 aatcctttct aaaatgagga gcgccattgt tagcagatcg aactttgcgc gatttgcgcg
     3361 agaactcagg atggataaag agttattact agggaagggt gaagagctta ccggcggaag
     3421 ggaaagggaa tctaacctcg ctggtgtatt tgaggctgtt atcggggcag tttatcttga
     3481 ggctggatat agaaaggctt ttcaaataac ctcacggctc ataaaaagtt gccttgaaaa
     3541 gcgagagata tttatggact ataaaacaac ccttcaggag ctagtccaaa aaaaatctgg
     3601 aagcacacca agatataagg tggttcttga agagggcccc ccacatagca aatatttcca
     3661 cgttgaagtg aaggttgcaa gaagggttct agggaagggt tcaggaagga gcaaaaagga
     3721 ggctgagcag gctgcggcta agaaagggct tgaaaatctt gagacgtcag gtcaagcaga
     3781 atcttctaga tgaattccaa aatcatctaa ctttttcgca agcgtatttc tatttattcc
     3841 cagaatttta gcggccttct ttttattacc cttggctatt ttcagaactg cctcaataag
     3901 tggtttttcg acacttctca taatcaaatc gtagattcca ttaggaggag gttcctgagt
     3961 taaatctatc atatttaata accttgtggt tattaactta ttgagtgaat cctcttcttt
     4021 cttttttatc ccctctaagt aaggagctgc ttcaagaaga acttgagatg tgatcatcac
     4081 atcggaagaa agaacaagca cccgctttat agtgttttca agctccctta cgtttccata
     4141 ccagttgtat cctagaagta tttcttttgc ttcatcggaa attattctct tgccgacgcc
     4201 aagttccttg ccatatttct caagaaaatg ttctgaaaga acaggaatat cctcttttct
     4261 ttctctaaga gaaggaagct cgattgttat tgcgttaagc ctatagaata ggtctcttct
     4321 gaaagaccct ttttcgactt ccttttcaag gtttttattc gttgatgcaa ttactctaac
     4381 atcaacctct acaggttttt cagcaccgag tctgtagaac tgtttttcct ctaatactcg
     4441 taaaagcttt gtctgaagct caggagtcat atctcctatc tcatccagaa gaagcgttcc
     4501 tctgtcggca tcttcaaacc ttccttgttt tcgaatcgtt gcccctgtaa aggcaccctt
     4561 ctcataccca aaaagctcac tttcaaggag ctccttagga agtgctgcta tatttacagg
     4621 tacgaatctt tcatcccttc tttggctgtt aaagtgtatt gcacgggcga cgagctcctt
     4681 tccagtcccg ctttcccctg taataagaac agttagatcc ttctcagcta ctcgccctat
     4741 agtcttataa atcttgagca tcgcgggaga tttgccaact ataccctgat gcggttcaga
     4801 tgatttccct tctctcagag tagcgcgtag gttttctaac cttttagaat tttcatgatt
     4861 ctctatagct ctttcaacag ttagttttac ctcatccagg ttgaagggct ttgctatgta
     4921 atcataggcg ccccgtttca tcgcttcaat tgcatttttt acggtgtttt gggcggtgat
     4981 gataattaca gggatgtcaa tcccttttac ccgcagttcc tctaggactt caaggccgtt
     5041 caaatctggc atttttatgt cgagtataat caatgaaaaa ttatctaagg ttgctttctg
     5101 aatagcctct gagccattct caccaaattc tactgtatag cctgccttct ccagacattt
     5161 tcccagaacc catctaatac tctcttcgtc gtctactact aaaacccttt gcttcatcct
     5221 aatataaaaa taccttacat tatcactatt agaagtctta agatttggtt gtcataagac
     5281 ataatttaga aacgctaact ttgaagtaaa ttactaccta atgacttcga gtaatatact
     5341 gtctgtactt ataattaaaa tctagacacc gctattccaa atttcttcat ttctatatat
     5401 cggaagatat accttaaaca ccgtgccttt tcctagctca ctttctacaa aaatagctcc
     5461 gccgtgcttg gctataattt gatatgcaat aaaaagcccg agccctgacc cctctttctt
     5521 agtagtgtaa aaaggtttat atattttttg gaggatctct tttgagattc cagatccggt
     5581 atccctaatc tctactaata ttgccttttc acccttaagt ttgtaatcag tgatccatct
     5641 tgttgtgatt tcaatgctcc ccctattagg gattgcttga atagagtttt ttataatgtt
     5701 tagaaaaacc tgcttgagag aatctttatt cccaagaacc gggggcagtg taatatcaaa
     5761 attctcaaca aatttaaccc tcttaccatc tcttgatttt gactctagaa atataatctc
     5821 tgaaataatg tcatgaatat ccacaggctc aaacacttct ttaggcagtg gctcaatctg
     5881 tcctagccta tcgagaagtg tgcaaagtct atccacttct tttgttatta tatctgcaca
     5941 ctttgcggta acgtctattg tcttttcttg aagtagtagc tgcgctgcac cttttattcc
     6001 actcaaagga ttttttaatt catgtgtaag tccgataaga agtccctcta atgttgaagc
     6061 ggaactctgc tgaagatttt tctcactaag gaactttgtg cttgataggt cttttatttg
     6121 gagaatcaca cctaaagcat tacttccatc tgaaaataaa ggggaggaaa ccacctgaat
     6181 tgagaccttt tctcctcctt ttaatactgg gttaatttca tcactaaaga ctgtgcgttc
     6241 atcttggatt gatttctctg caattgcctc catttcttcc ggcagaaccg tagaagaaga
     6301 cattccccgg gctttttccc tcgagatttt aaatatcgtc tctgctgcat gattcaagct
     6361 tatgatatta aaggatttat caagcgctac gattccatca ggaagcgcat caagtatgtc
     6421 ctggaaaaaa tcctccctgg ttttagtagc tctattcatt acgagagatc ttctatcctt
     6481 ataaaaacgc tttttgattt tataactggg aatctgtcta tctcctcaat agcagacatc
     6541 aaatctcttt cctttgcctc atgtgtcata atgactacag gaacctcacc cccgatatgc
     6601 cttcccttct gaattactga ttcaatgctt atgttatttc tgccgagact tccagcgatt
     6661 tgacccagta ctcccggtct gtcttctacc tgaaacctta aataatactt tgtagttatt
     6721 tcactaatag gaactagacc agagggaatt gaagtcgttc cttgtaagga ttttggcagc
//
LOCUS       DATDRL010000339         3655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2834837, whole genome shotgun sequence.
ACCESSION   DATDRL010000339 DATDRL010000000
VERSION     DATDRL010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2834837"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VI935_11310"
     CDS             complement(<1..122)
                     /locus_tag="VI935_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HLE26216.1"
                     /translation="MIKDAIAKVAERIDLEEKEMAQVMENMMGGTASPSQMAAFL"
     gene            complement(100..564)
                     /gene="nusB"
                     /locus_tag="VI935_11315"
     CDS             complement(100..564)
                     /gene="nusB"
                     /locus_tag="VI935_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HLE26217.1"
                     /translation="MGKRRRARENTLQFLYQYDTLKESSSQELSTDDVLMLFWATKES
                     DVEEEVREFSNRLITGACENIEGIDDIINRYSEHWRLSRMSKVDRNILRMAIYELVYL
                     RDIPPPVTINEAVELAKKYGTEESGAFVNGILDRIRVALDKGEVEYDKRCDS"
     gene            complement(574..1050)
                     /gene="ribE"
                     /locus_tag="VI935_11320"
     CDS             complement(574..1050)
                     /gene="ribE"
                     /locus_tag="VI935_11320"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HLE26218.1"
                     /translation="MTRIYEGKLDGKGLKFAIVVSRFNNFITERMVEGALDVLIRHNV
                     SEGDIDIIKVPGSFEIPLGVKKAVKTNKYDAVIAIGAIIRGETPHFDYLSSEITKGLA
                     GISLEYDIPVAFGVITTETLEQAIERAGSKAGNRGGEAALSAIEMANLGRVLSQKR"
     gene            complement(1065..2273)
                     /locus_tag="VI935_11325"
     CDS             complement(1065..2273)
                     /locus_tag="VI935_11325"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HLE26219.1"
                     /translation="MGICPVEEAVEEIKNGRMVILVDDEDRENEGDLVVAAEFASPEA
                     INFMAKHGRGLICLALTQEKANQLNLHPIKPENSTVPQYTAFTIPIDARRGITTGISA
                     YDRASTIRLAISEEARPEEFIRPGHVFPLISRRGGVLVRAGHTEGSVDLVRLAGLKPA
                     SVICEIMKDDGDMARLPDLERFAEEHKLIITTIADLIGYRLKAERLIKRSAGAYLPTS
                     YGDFKVIVYESDIAPQHHVALVKGDISPEDEVLVRVHSECLTSDVFGSMRCDCGPQIH
                     QAMNTIEKEGKGVVLYMRQEGRGIGLVNKIKAYALQDDGFDTVEANEVLGFKPDLRDY
                     GIGAQILLDIGVRKMKLLTNNPRKVKGLEGFGLQIVERVPIEIEPNGANSRYLKVKKD
                     KLGHILSMVD"
     gene            2464..2631
                     /locus_tag="VI935_11330"
     CDS             2464..2631
                     /locus_tag="VI935_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26220.1"
                     /translation="MNPEIVILVKDDEVVKEEDWEEEDEEDEELWDEEEDEDLVDDED
                     DDWEEEDEEDS"
     gene            complement(2815..2900)
                     /locus_tag="VI935_11335"
     tRNA            complement(2815..2900)
                     /locus_tag="VI935_11335"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2864..2866),aa:Leu,seq:taa)
     gene            complement(3139..3210)
                     /locus_tag="VI935_11340"
     tRNA            complement(3139..3210)
                     /locus_tag="VI935_11340"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3176..3178),aa:Cys,seq:gca)
     gene            complement(3467..>3655)
                     /locus_tag="VI935_11345"
     CDS             complement(3467..>3655)
                     /locus_tag="VI935_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26221.1"
                     /translation="DAYRKISINSLELKVNGAVPRETVNLRIYPMSNGISEVRFWCNG
                     KLIDVKRVKNSELNIVHF"
BASE COUNT         1055 a          815 c          657 g         1128 t
ORIGIN      
        1 agaaaagccg ccatctggct tggcgatgca gttccaccca tcatgttttc cataacttga
       61 gccatttctt tttcttcgag gtctattcgt tctgcgacct tagctatcgc atcttttatc
      121 atattcgacc tcccctttat ctagagctac tcggattcta tccaaaattc cgttaacaaa
      181 agcacctgat tcttcagtgc catatttctt ggcaagctcg acggcttcat taatggtaac
      241 tgggggagga atatccctca aataaacaag ctcataaatt gccatacgaa ggatatttct
      301 atcgactttt gacatccgtg aaaggcgcca gtgttcagag tagcgattaa taatatcgtc
      361 aatcccctca atattctcac atgccccagt aataagcctg tttgaaaatt ccctcacctc
      421 ttcctctaca tctgattcct tagtagccca aaaaagcatg agtacatcgt ctgtgcttag
      481 ctcttgagaa gaggattctt tgagggtgtc gtactggtat agaaattgaa gagtattttc
      541 tcttgcacgt ctccttttac ccataaccgc atactatctt ttttgagaaa gcactcttcc
      601 tagatttgcc atctctattg cagaaagggc tgcttctcca ccacgatttc cagccttgct
      661 tccggccctc tctatcgcct gttcaagtgt ttccgttgta ataacaccaa atgcgacagg
      721 tatatcatac tcaaggctga ttccggcaag cccttttgtg atttcagagg aaagataatc
      781 aaaatgtggc gtttcacccc ttatgattgc tcctattgct ataactgcgt catatttgtt
      841 tgtctttaca gcttttttta cccctagagg gatttcaaat gaccccggaa cctttattat
      901 atcaatatca ccctcagaaa cgttgtgtct tataagcaca tccagtgcgc cttcaaccat
      961 acgttctgtt ataaaattgt taaatcgact aactactatt gcaaacttaa gcccctttcc
     1021 atcaagttta ccttcataaa ttctagtcat ttggttcctc acctttagtc aaccatagaa
     1081 agaatatgtc caagcttgtc cttctttacc tttaaatacc ttgagtttgc accgttaggt
     1141 tcgatctcta tcggaacccg ctcaactatc tgaagtccaa atccctcaag cccctttact
     1201 ttccttggat tattagtaag aagcttcatt tttctaaccc ctatgtctaa aagaatctgc
     1261 gcgccaattc cataatccct cagatctggc ttaaaaccaa gcacttcgtt agcttctacc
     1321 gtgtcaaatc catcatcttg gagtgcgtag gctttaattt tattcactaa tcctattccg
     1381 cgtccctcct gccgcatata gagaacaacc cccttcccct ccttctcaat tgtgttcatg
     1441 gcttggtgaa tttgtgggcc acaatcacat ctcatagaac caaacacatc gctagttaga
     1501 cactcggaat gaaccctaac cagcacctca tcttcgggcg aaatatcgcc ttttacaagg
     1561 gcaacatggt gctgaggggc tatatcactt tcataaacaa taaccttaaa atcaccataa
     1621 gaagttggta aataggcccc tgctgacctc tttataagcc tttcggcttt aagtcggtag
     1681 cctataaggt cagcaattgt cgtaattata agtttatgct cctcggcaaa tctttctaga
     1741 tcgggaagcc ttgccatgtc gccatcatcc ttcattatct cgcagattac agaagccggt
     1801 tttaaacctg caagccttac aagatccact gagccttctg tatgccctgc tcttacaaga
     1861 acgccacctc gcctagaaat aagcgggaat acatgaccgg ggcggataaa ttcctcaggc
     1921 cttgcttctt ccgagatagc cagacgaata gtagatgccc tatcgtaagc agatatccca
     1981 gtagtaatac cgcgccttgc atctattgga attgtaaaag ctgtatattg aggaacagtg
     2041 ctattttctg gctttatagg gtgaagattg agttggttag ccttttcctg agtgagggca
     2101 aggcaaataa ggcctcttcc gtgctttgcc ataaaattta ttgcctcagg gctcgcaaac
     2161 tcggcagcaa caacaaggtc accctcgttt tccctgtctt catcgtcaac gaggattacc
     2221 attcttccat tttttatttc ttctacggct tcttcaaccg ggcagatccc cataccttaa
     2281 cctctcaatt gctataggac ttatcccaat aaattataag ctaaaataat aagcaaccaa
     2341 gtaagtttgt caactaaagt catttgctgt caaatttcaa ctaaggataa attagttttg
     2401 agagcttgac ttttcaatgc taaaaggtat ggtttggaat atttattaat taggagagac
     2461 tatatgaatc ctgagattgt aatcctagta aaagacgacg aggtggttaa ggaggaagat
     2521 tgggaggagg aagatgaaga ggacgaggaa ctttgggatg aggaagagga tgaagacctt
     2581 gttgatgacg aagatgatga ctgggaagaa gaagatgaag aggattcata aatctagaga
     2641 atctttacta atcagttctt atggcttaat taagagcatt gtaataaacc caaataccta
     2701 acactagtca caaagaagac cctgaaattt aagtaaatgg ccactttgaa aaaaacacca
     2761 cttatataga catccccccc gaaaacctta tagaacttat aaatcagact aaactgccca
     2821 gggccggact cgaaccggca cggaccttac ggcccattgg attttaagtc caatgcgtct
     2881 accaattccg ccacccgggc gatagacaaa tgtttttatt tttaccaaaa ttaaagagaa
     2941 tttcaaatta ctctacacaa ttaatatttg atactagtca ctaaaagcca cttaacatgg
     3001 ttttcggaca tattggtagt aaattacgaa ttatatggaa cataagttta ttctgcaaaa
     3061 cgaatgaaga cctgtgtagt aaaatcgtta tattgaatta ccaaatgatt ttttatgaat
     3121 cttgataatc tcgatttgag gcggcgcccg gattcgaacc ggggtattgc ggttttgcag
     3181 accgctgcct tacctcttgg ctacgccgcc ttgttcaaga aggtagtgta taaaattgtg
     3241 actcatccgg atgaggaatt tatcagtgtt aatttttaat agtctgaccg aatttttaga
     3301 tgttttcaag ttgatcttca accacaacca aataggaata gtataatagg gaggctttgg
     3361 atcataattt tggttgtaaa attagtttat atctatcata gctttctatt caagggtaag
     3421 aagtttcatt atgttagcta aaatttaaaa gtgaactctt taagaattaa aagtgaacta
     3481 tatttaactc actattttta acccttttga catcaataag tttaccattg caccagaatc
     3541 ttacctcaga tattccatta ctcataggat aaatacgaag atttacagtc tctcttggga
     3601 cggccccgtt aactttaagc tctaggctat tgattgatat ttttctgtaa gcgtc
//
LOCUS       DATDRL010000340         2835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3750947, whole genome shotgun sequence.
ACCESSION   DATDRL010000340 DATDRL010000000
VERSION     DATDRL010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3750947"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /gene="cimA"
                     /locus_tag="VI935_11350"
     CDS             <1..1010
                     /gene="cimA"
                     /locus_tag="VI935_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HLE26222.1"
                     /translation="KLQNPPLGIHTHNDGEVGVANTLYAVRLGVGQVQGTMNGYGERC
                     GNANLCSIIPNLKLKLGINCIDDDELRKLYEVSHFIHELTNLPPIKKLPFIGESAFAH
                     KGGLHVSAVMKKSETYEHINPEIVGNRRRVLVSDLSGKSNIIYKAKELGIELDPSDRK
                     VQKILNELKKLENQGYEFEGADASFELLVRKTLGQYRKHFELKGATIIVEKHRDDEEP
                     ISEATVIVEVDGVLEHTAASGNGPVNALDNALRKALEKFYPALKEVKLKDYRVRVVST
                     GKGTDAVVRVLIESGDGENDWGTVGVSENIVEASWKAMVDSIDYKLLKDEAKRAEKAR
                     G"
     gene            complement(1182..1721)
                     /locus_tag="VI935_11355"
     CDS             complement(1182..1721)
                     /locus_tag="VI935_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26223.1"
                     /translation="MKVETLELLQKLQKKFGIKGTPSGPFGKIVQKLLALSLHEMGFT
                     NIVERGVQGVDIDITSESNQKFAFEVKTTEKLSIPLTPDNVQALRDRTHDGYQPVIAV
                     LRLAPFENWILAKIPMDEIPTGEMLIESLKPYGMEDLGSQLCPKFDIVVRKHFKDVYN
                     RGEQYLKEQLRKVGIMAKD"
     gene            complement(1725..>2835)
                     /locus_tag="VI935_11360"
     CDS             complement(1725..>2835)
                     /locus_tag="VI935_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLE26224.1"
                     /translation="EKEIKRISSKAKLIEEGISILESAVPFDAEICPLCGGKVSNLLE
                     RLRKDWKEKIEAQVQELKKQRKELDEKKTDFENSEKKLVRLEEDTQTEKGELRKAINN
                     ISEFLHKVLNDKDDPAVILSIEIEKINSGLKEIEDAIKSKRSRIEDISKETDKMSLLY
                     DILLLKNKLEEINQIGSTDEYQKQEKIKTDVSNLIRYVEELSKIIKSCMLKEAEEKIA
                     LARSAIDYYFRKITDNPAFQKLNIKVEEDKKTGGNFYIFEDQDGKKLVPILSQGDLNS
                     IALSIFLGLGKTAGDSHPLGFILMDDPSQSLDAQQKTHLVEILNELSDIKNITVSTMD
                     TELQQFLKDGITKVKTIYKFSDWAPDSGPKASKEI"
BASE COUNT          811 a          567 c          561 g          896 t
ORIGIN      
        1 caaagctcca aaatcctcct cttgggattc atactcataa tgatggggaa gtaggtgttg
       61 cgaatacgct ttatgccgtg aggcttggag taggtcaggt tcagggaact atgaatggat
      121 acggtgagag atgcggaaac gccaacctat gttcaatcat accgaatctc aaactaaaac
      181 tagggataaa ttgcattgac gatgatgaac ttagaaaatt atacgaggtt tctcatttca
      241 tccacgagct tacaaacctt cctccaatca agaagcttcc tttcattgga gaaagcgcat
      301 ttgcccacaa gggaggattg catgtaagtg ctgtaatgaa aaaatctgaa acctatgagc
      361 atataaaccc ggagattgta ggaaatagaa ggcgtgttct tgtctcagac ctttcaggga
      421 aaagcaacat tatctataag gcaaaagagc ttggcattga gcttgatcct agcgatagaa
      481 aggtgcaaaa gattcttaat gagcttaaga agcttgagaa tcaaggatac gaatttgagg
      541 gtgctgatgc atcatttgag cttcttgtaa gaaaaacact cgggcagtat aggaagcact
      601 ttgaactcaa gggcgctacg ataattgtag aaaaacatag agacgacgaa gaaccgattt
      661 ctgaggctac ggttatcgtg gaagtagacg gagtacttga gcacacagcg gcaagcggta
      721 acggtccagt caacgcactt gacaacgcac ttcgtaaggc acttgaaaaa ttctaccctg
      781 ctctcaagga agttaaactt aaggactata gagtccgtgt ggtctcaaca ggaaagggga
      841 cagatgccgt ggttagggtt cttatcgaat caggagacgg cgaaaacgac tggggcacag
      901 tcggggtttc agaaaacatc gtcgaggcaa gctggaaagc gatggttgac agcattgatt
      961 acaagttgct aaaggacgaa gcgaagaggg cagagaaggc tagggggtaa ttaccgaagc
     1021 ctccgtttgt tttttcttaa acctgaaaaa gccaattagg gatatatttg cggataacgt
     1081 ccttggtgca aaagaagttc gaagggcttg gattttagtc ttttgcacca agttatatga
     1141 agtcgccgta acataaaatt tccaattcca acattaagtt actagtcctt ggccataatt
     1201 ccaacttttc tcaactgttc cttaagatat tgctcacccc tgttgtatac atccttaaaa
     1261 tgtttccgga ccacaatatc aaacttagga catagttgac ttcccaaatc ctccatcccg
     1321 tagggcttta aactttcaat caacatctct cctgttggga tttcatccat cgggattttt
     1381 gcaagaatcc agttctcaaa aggggcaaga cgtaagactg ctataacagg ttggtaccca
     1441 tcgtgagtac ggtcacgaag ggcctgcaca ttatctgggg taagaggaat tgataatttc
     1501 tctgttgttt ttacctcaaa tgcaaatttt tgattagatt ccgatgttat atcaatatca
     1561 actccttgca cgcctcgttc tacaatatta gtaaagccca tttcatggag actgagagcc
     1621 agtagttttt gtacaatttt gccaaaaggt ccggatggtg tgcctttgat tccaaacttt
     1681 ttctgcaact tctgtaaaag ttcgagagtt tctactttca tagtttatat ttccttcgaa
     1741 gctttcgggc cggaatccgg tgcccaatcc gaaaacttat atatagtttt gactttggta
     1801 ataccatcct tcaaaaactg ttgaagttca gtatccatag tagagactgt aatgtttttt
     1861 atatcgctga gttcattaag gatttctaca agatgggtct tctgctgtgc gtccaaactt
     1921 tgcgagggat cgtccattag aataaatcca aggggatgag aatccccagc tgtcttccct
     1981 aaccccaaga aaatggataa agctatgctg tttaagtctc cttgacttag aataggaaca
     2041 agtttcttac cgtcttggtc ttcgaaaata taaaagtttc caccagtttt tttatcctct
     2101 tcaaccttaa tattgagttt ttgaaaagcc gggttatcgg ttattttacg aaagtagtag
     2161 tcaattgcac tcctggctaa agcaattttt tcttctgctt cctttagcat acaagatttg
     2221 ataattttac taagttcttc cacatatcgt attagatttg agacatcggt tttgattttc
     2281 tcttgtttct ggtattcatc ggtgctccca atttggttta tttcttccag tttattcttc
     2341 agtagcaaaa tatcataaag taaactcatt ttatctgtct ctttagaaat atcctcaatt
     2401 ctggatcgct tactctttat agcatcttca atctctttta gacctgaatt aattttttct
     2461 atttctatgg ataatattac agcaggatca tccttatcgt tcaacacttt atgtagaaat
     2521 tctgagatat tattgattgc cttccttaat tccccttttt ctgtttgagt atcttcctct
     2581 aagcgaacaa gtttcttctc tgagttttca aaatcagttt tcttttcgtc caattctttc
     2641 cgttgtttct ttaactcctg cacttgggct tcaatttttt ctttccaatc ctttcgtaac
     2701 cgttcaagca aattggacac ttttcctccg cataagggac aaatctcagc atcaaaggga
     2761 acagctgact ccaaaataga aatgccctct tcaattaatt tcgctttcga gcttattcgc
     2821 ttaatttctt tttca
//
LOCUS       DATDRL010000341        18621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2206534, whole genome shotgun sequence.
ACCESSION   DATDRL010000341 DATDRL010000000
VERSION     DATDRL010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 18621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18621
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2206534"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2108)
                     /locus_tag="VI935_11365"
     CDS             complement(<1..2108)
                     /locus_tag="VI935_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26225.1"
                     /translation="MDGGSLLEKKLITLTLIIFTFLCFLFLGTRVQADTYCVGCPGAS
                     AANCDGNAACGAPCDDPMTMGFNCPTIENALDCIATNMDPDATIRIAQNPTMPLTPYT
                     GPGSTNPIDNTAMMMDQEINIQGGWDAMGCTSQTLPLDPTNTIIEAPSMDRVFLIDNT
                     TSDLLTVTLQGLTITGGNPTGNCGPGMLSGPNGAGVCATSTSGSGGLNYTSISNIYKE
                     NNATSPQGKGGGLAVIAGGGEDIVFVSGMMMGGGDTFTNNTADEDGGGILLAATGAML
                     DATIHNATIGGDDPFPNGGNEGNQGGGMSIRGEGGSVIVSNQGNLIANNTAVDGFGGG
                     GVIIINKSGNTEPVTTSISEDEITFNAADGDHGGGITIEAESNTTINTSISKTEIGNN
                     NAGTFGGGIALRGDGNINLNPVVNNIIHHNIAEGSPLGGGGVAAVTSGADANFDIKFV
                     NNTIADNQAPNAGGQGGGILADNTANGVINWTLPNDIIFFNISAGTGDQIALVENGTT
                     CMPPSPADCATIAVRFTDISNALGDIVGIGTGVGFVNLVDPNIFNIDPQFIDHFNGDY
                     RITSDSPVATAGTKTPILTIAGVEADPPDFDIVGTPRDISMGPYEPVPSPTPTPTPTV
                     SPTPSPTPTTPPTPTPTPTIPPPTPTPTPQDTLTFPNANNGKIVTIESEPGTTIQGGP
                     LGKTLNGCPEVFNDQFLTFPV"
     gene            2762..4348
                     /gene="serA"
                     /locus_tag="VI935_11370"
     CDS             2762..4348
                     /gene="serA"
                     /locus_tag="VI935_11370"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HLE26226.1"
                     /translation="MRILITDGLAEEGLNLLKSQPELEVVMKKGLPKEEIKGIIKDYD
                     ALIVRSGTKVTADIIEAAGERLKVIGRAGIGVDNVDVAAATRNGTVVMNTPEANAITT
                     AEHTIALMFSLVRKIPQAHLSVKSGLWEREKFKGKEIFGKTLGIIGLGNIGRLVAERA
                     IGLKMKVIAYDPFLTQEAAVKMGVELVSFNELLGMADIITIHTPLTPETKNLIRKETF
                     LRMKKGVIIINCARGGIVNELDLYEAVKSGIVGEAALDVFEKEPPGKDNPLLKLDELV
                     FTPHLGASTEEAQTKVSVAIAEQIVDFFINGAVRNAVNMPSISIELLKIMKPYIVLAE
                     KLGSLQGQLCRSGVEEINIEYSGEVAGFDVAPLTIAALKGFLTPIMDITVNYVNAPFV
                     ARERGIRVIESKSSRSEDFTSLISIKVKTNIDEKHVSGTIFGREEPRFVRVNGFSLDV
                     IPQGYLLISENYDKPGFIGAMGSLLGKRGVNIARLHLGRESVGGRAIAFINVDSPIPS
                     GVMEEISALPNIISVTQVVL"
     gene            4349..5596
                     /gene="hisZ"
                     /locus_tag="VI935_11375"
     CDS             4349..5596
                     /gene="hisZ"
                     /locus_tag="VI935_11375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HLE26227.1"
                     /translation="MLERFSLPQGVRDFPPEGAEEIRRVEECLLDEFTRWGYRRVITP
                     FFESLETISVGLGDDLKDKILKFVDPSTGDVVALRPDITPQVGRIVATQLRDHMGPLR
                     LCYNGRVIRYEERGSGKEREIFQVGCELMGLSSPEGDGEIIALAVKSLEKLGIKNLVL
                     DIGHTGLVRFLLGKTGNVRNEIEEALKKKDQEALEKATSKAPIPQAIKRVILSLPELY
                     GNKDILRRAKKISIIRKYVKELEDVLILLDEFQVGCEINVDLGELRGFNYYTGVSFEL
                     ITPRVPSPMIIGGRYDELIEKYGYKCPATGFAVDIEALLDVATSTSYEDNKNHFVVIP
                     TKPSLRRESIKIAESLRRSGFKVILDLKADVNLVRERIKENRSLSSYGVILLETFGKL
                     RLIESRTGVSKEFSNLEELLSSI"
     gene            complement(5668..5937)
                     /locus_tag="VI935_11380"
     CDS             complement(5668..5937)
                     /locus_tag="VI935_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26228.1"
                     /translation="MGQIVRFLCMKQVSQSPKQSYVGTECHCEENYFERSVREMEIKG
                     SDEANPKERLLRRVWGSYPEEGHLGNRVARNDSENPLASQRELSC"
     gene            6042..7334
                     /locus_tag="VI935_11385"
     CDS             6042..7334
                     /locus_tag="VI935_11385"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HLE26229.1"
                     /translation="MPNIVVVGAQWGDEGKGRIVDLIAERVDVVTRYQGGSNAGHTIV
                     TNDKTLVLHHVPSGILREGKLSVIGNGVVVDPKVLLEEIEKIKSHGYKVDEENLKISG
                     SAHIVMPYHKVIDLAREERMGSGKIGTTGRGIGPVYEDKAARRGVRFSDLIYPEGFSL
                     RLRSILEERNLYITRVLGRNPLEFEKIYEEYVIYGKKLRMFITDTPKVLNDAISQGKS
                     VLFEGAQGALLDVDFGTYPYVTSSSAGAGGACIGTGVSPTKIDIVMGVAKAYTTRVGE
                     GPFPTEVLGDLGIKLRELGNEYGSTTGRPRRCGWFDSVALRYSSMVNGVTGLAITKLD
                     VLSSLDKIKICVGYRFKGELLTEFPSNTEVLKNCEPVYEELDGWREELRDLKNLSSLP
                     PSARAYLERIEETTGVPIWILSLGPSREKVIFLRDIFS"
     gene            7479..8870
                     /locus_tag="VI935_11390"
     CDS             7479..8870
                     /locus_tag="VI935_11390"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HLE26230.1"
                     /translation="MRLFLGAGLAIAMLLTLPFAKDSRAQSASEQIKALKKQLEEIQK
                     QNQQQIEELQQKIQDLEVKKEAEKEKIIEEIKAEQKDAWYNKIEVSYKKPGDGFTIKT
                     KDGNFSLNMRLRAQFQFSVNDTTDELTATDFRIRRLRFYWIGNAFRPWFEYYIQVSAD
                     NGSDLQIRDAYFNAAYEKVAIPRVGQFKVPFNREELNSSSELQLVERSIVNEEFSLGR
                     DIGPALYGLLGNYVTYGVGIFDGNGRNDVSTDSNLLYAGRIMLTPCCGELKYANSSFP
                     IGGDYKIEPNFGEDKPLIAFGVAAAGMEGLNIDIKTPDASVDDRFEAIGITVGDFVQL
                     TADANFKYKIFSIEGEYDARWISPDANQGVSTDTVFDQGFRLQSGVFLIPRLVEVAGR
                     FSYIDFDASVPNIQGVDEPDNSWQITPGLNFYMSHSHKWKIQLDYSFIKNEFTNADDV
                     DENIFRAQLQAYF"
     gene            9156..9779
                     /locus_tag="VI935_11395"
     CDS             9156..9779
                     /locus_tag="VI935_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26231.1"
                     /translation="MSLEKLTEGWVLALQLYGSAKTVSFSDHNFTNGSLTSLTGGRER
                     NTFISREFGFRGSWPNGWMIDNKLNELYKRQFGFRDKVVIPIVVIPQSLYGGFRPSVT
                     VAMDDSVGIDINSYIENNLLVSKDALVEIIKSIGVDIASILQFHYLSLYGVSSEERIF
                     QIQKVALTNNKAYIVTASQIPESELKTNKRLRGDLDKIMTSFSIVSF"
     gene            complement(9762..10187)
                     /locus_tag="VI935_11400"
     CDS             complement(9762..10187)
                     /locus_tag="VI935_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26232.1"
                     /translation="MKREAFLLTSSILLVFLGCAREWQDPNTTIPAKKVDIAAILENP
                     IAFDSAGVIVEGKIWDLVFDKFEREDKETPFTEFKLADQDGNFVNIFVLGHIPIAEGD
                     IVEVMGIYRRELGKERIYFANEIEAIRVEKEKQELKANN"
     gene            10450..10725
                     /locus_tag="VI935_11405"
     CDS             10450..10725
                     /locus_tag="VI935_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26233.1"
                     /translation="MSIKIYNSETKELLGEIKSYSISRFYVTGIDIIWGIWVDAPFTD
                     PNEQKAAFTLQATNDNGKEITFEDGWLKDVTGTRPNIAVRITAKKKE"
     gene            complement(10744..11721)
                     /locus_tag="VI935_11410"
     CDS             complement(10744..11721)
                     /locus_tag="VI935_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HLE26234.1"
                     /translation="MQETLMAENGITRELNFEDNGVLKQLYGENNVNLKEIESVFGVK
                     IHSRGGKLSLKGDPEVVESVERFLNEIYRLISKGYSLDPGDIELAARVVIEDKTPLED
                     IFLDTVCISTRKRLIAPKSLAQKLYIDAIRKNDIVFGIGPAGTGKTYLAMAMAVSAFI
                     SREVNRIILTRPAVEAGEKLGFLPGDLYQKIDPYLRPLFDALYDMLDFEKATRLIEKG
                     AIEIAPLAYMRGRTLNDAFVILDEAQNTTAMQMKMFLTRLGFSSKAVITGDVTQVDLG
                     GNEKSGLLEAESLLKDIEGIVIVHFSRRDVVRHPLVRQIIDAYERKEKD"
     gene            complement(12672..13709)
                     /gene="msrB"
                     /locus_tag="VI935_11415"
     CDS             complement(12672..13709)
                     /gene="msrB"
                     /locus_tag="VI935_11415"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HLE26235.1"
                     /translation="MQTRIYADNKTEKATFAGGCFWCMEPPFEKLDGVIDVISGYTGG
                     KKANPTYEEVSYGGTDHLESVQVIYDTSKITYSELLDVFWKQIDPTDPNGQFIDRGSQ
                     YRSAIFYHNDEQKSLAEKSKEELDKTGKFGKPIVTEIIMASAFYKAEDYHQDYYKKNP
                     IRYKFYRYNSGRDQYLKKIWGDEMEKKVSKNNNEEYKKPSKEELRKKLTPLQYKVTQE
                     EATERPFANEYWDNHRDGIYVDIVSGEPLFSSLDKFDSGTGWPSFTKPLGEGNIIEKE
                     DRSFFTVRTEVRSKNADSHLGHVFDDGPKPTGLRYCMNSASLRFISKEDLEKEGYGQY
                     VKLFENKTSKE"
     gene            complement(13814..14287)
                     /locus_tag="VI935_11420"
     CDS             complement(13814..14287)
                     /locus_tag="VI935_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26236.1"
                     /translation="MYKILILTLLLPLTFSFIQTSANAATNLKLNPNLDYSSDTNDGP
                     LIIGDNITDGMIQQGKPAYIIFYHHECYNSKRQAKRTVELYEKYEGNIDFIIVDLDSK
                     ISNEQNELRERFYRNYIPHVTIIGANGSVLYNRSGEIGTDLLSSILDKALMENEK"
     gene            complement(14433..14705)
                     /locus_tag="VI935_11425"
     CDS             complement(14433..14705)
                     /locus_tag="VI935_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HLE26237.1"
                     /translation="MAKKAQLRRVNPKKVDTCKQATALILDYLTGELDPRTALLFEEH
                     LMVCPDCVSFLNSYKETIKFTRSLSYENIPPDMKKRIRRFLRDNFR"
     gene            complement(14798..15427)
                     /locus_tag="VI935_11430"
     CDS             complement(14798..15427)
                     /locus_tag="VI935_11430"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLE26238.1"
                     /translation="MTKKELSGKGQLSGEDERLLIQRVKAGDQKAFEAIFNHYVSRVY
                     RQAFKFVGNEADAEDVVQDVFLLVYRKANSFLGKSEFSTWLYRLTANAALTKLRQRKK
                     QKEVSLDEYLPKFRKDGHHLVRPVVDWTQEVDKFVAIKEYSQIIKEAMEKLSPMDKAV
                     VVMSDMEEMSNPEISATLGITVQAVKARLHRARLFLRGKFTAQLGYSPT"
     gene            15806..16525
                     /locus_tag="VI935_11435"
     CDS             15806..16525
                     /locus_tag="VI935_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreH"
                     /protein_id="HLE26239.1"
                     /translation="MTSPTLIATLGLGFILGLKHALDADHIVAVSTIVSEHKSISKSS
                     LIGTFWGLGHTVSLFLIGLLVILLRLVIPPKLALLMEFGVAMMLVFLGANIIWKFFRE
                     RKIHIHSHEHDGVPHAHIHLHEQGREEAHEHNHLIKFGSKPFLVGLVHGVAGSAALML
                     LVLTTIPSPLTGLLYIVVFGVGSIGGMLLMSSLIGLPFVFTANKFASMNQAIKVLAGT
                     SSIAFGVYLGWEIGFLQCLFP"
     gene            16617..17414
                     /locus_tag="VI935_11440"
     CDS             16617..17414
                     /locus_tag="VI935_11440"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HLE26240.1"
                     /translation="MSAAKACLLIGLFIELSTSISFAFRPFRTEDAGISPIGFFAVEL
                     GNEFRKTHDGKDNVTTFALTYGLLDWIETEIDFALLTLRPDSGKNEFGFGDTVLLSKF
                     KILGEKGVLYDDDLFPDMVIRPSVLIPTGDEDKGTGSGQIELGILLVLEKTFWKVGSR
                     ANIGYLASNDPTFDENFEDRFFYGVQADVPLFTERLRFGSEITGQFASGEGSPLFSLT
                     GLVFQVTENIVLDGGVELGLKNAGSAVTVIVGITFGFNPFEVFLTNR"
     gene            17708..>18621
                     /locus_tag="VI935_11445"
     CDS             17708..>18621
                     /locus_tag="VI935_11445"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HLE26241.1"
                     /translation="MLKGSKFLVTFFLPLILTLLFTFSAISWAEYNDITAEEIIEHIR
                     FLASDQLEGRKAGTVGCEKAAEYIASHFKEAGLKPLGDNGTYFQNFSFTSGVKLGESN
                     SMTVDMGEEKLDLQVGKDFLPLSFSSDGELRGELVFAGYGISSKQLSYDDYAGIDVKD
                     KIVLVLRYTPDGNNRKSPFYKYASLRYKAINAREKGAKGIIFTTPLSQKEEEGLGGIR
                     FDRSFTDSGVHAIILRREIAQEVLKTAAKDMKTLEQKLGEKKPSSFAIPETEAQIHTE
                     LIREKSPTANVIGYIEGSDQSLKDQVII"
BASE COUNT         5444 a         3533 c         4000 g         5644 t
ORIGIN      
        1 acagggaagg ttaaaaattg gtcattaaaa acctctgggc atccatttaa agttttacct
       61 aaaggtcccc cttgaatagt tgtacctggc tctgattcta tagtcacaat ttttccatta
      121 ttagcatttg gaaatgtaag cgtatcttgt ggagtcgggg taggtgtggg aggaggaatt
      181 gtaggagttg gtgtaggagt tgggggtgta gttggggtag gagatggcgt tggtgaaaca
      241 gtaggtgttg gagtgggagt tggtgaaggc accggctcat atgggcccat tgatatatcc
      301 cttggggtgc cgactatgtc gaaatcagga ggatctgcct caacgcctgc tattgtcaaa
      361 atcggtgttt ttgtacccgc agtagcaacg ggagaatcag atgtgatgcg atagtcacca
      421 ttaaaatggt caataaattg cggatcaata ttaaaaatat ttgggtcaac taaattgaca
      481 aatccaaccc ctgtgcctat accaactatg tcacccagtg cattagaaat atcggtaaaa
      541 cggactgcta tagtagcaca gtcagcagga gaggggggca tacaggtagt accattctcc
      601 acaagtgcaa tctgatcccc tgtacccgca cttatattga aaaaaattat atcgtttgga
      661 agagtccaat ttattacacc atttgcagta ttatcagcta gtattccacc tccttgacct
      721 ccagcatttg gtgcctggtt atcggctatt gtgttattaa cgaattttat atcaaaattg
      781 gcgtctgccc cgctagtaac tgcggcaacc cctccgccac ctagagggct cccttcggcg
      841 atgttatgat gaatgatatt attaacaaca gggttgaggt ttatgtttcc atcacccctc
      901 agagctattc cgcctccaaa tgtacctgca ttattgttgc ctatctctgt cttgctgatt
      961 gatgtgttga tagttgtatt cgattcggct tcaattgtaa tccctccacc gtgatcacca
     1021 tctgccgcat taaatgtaat ttcatcttca ctgattgatg tagtaacggg ttcggtgtta
     1081 ccacttttat taataataat aactcctcca ccaccaaaac cgtctacagc ggtgttatta
     1141 gcaattaaat ttccctgatt agaaacaatt acacttcctc cttcacctct gattgacatc
     1201 cctccacctt gattaccttc attaccgccg tttggaaagg gatcatcccc accaatagta
     1261 gcattatgga tagttgcatc tagcattgca cctgtagcgg caagtaatat tcccccgcca
     1321 tcttcgtctg cagtgttgtt agtaaatgta tcccctcctc ccatcatcat ccctgacaca
     1381 aatactatat cttctccacc accagctatt acggctagac cacccccctt accttgtggt
     1441 gaggtagcgt tattttcctt atagatatta gaaattgatg tgtaattgag ccctccactt
     1501 ccagaggtag aagtagcgca tactcctgct ccatttgggc cggataacat tcctggtcca
     1561 caatttcccg taggattccc cccagtgata gtaagtcctt gtaaagttac ggttaaaaga
     1621 tcacttgttg tattgtcaat aagaaatacc ctatccattg aaggggcctc aattatagtg
     1681 tttgtggggt caaggggtag agtttgagaa gtgcaaccca ttgcatccca gccaccttga
     1741 atattaatct cctgatccat catcattgca gtattgtcaa ttggattcgt actacctggg
     1801 ccggtataag gcgtaagagg catagtaggg ttttgagcta ttcttattgt tgcatcgggg
     1861 tccatattcg tggcaataca atcaagagca ttttcaatcg ttggacaatt aaaacccatg
     1921 gtcataggat catcgcaagg tgcaccgcaa gctgcattcc catcacaatt cgctgccgat
     1981 gccccgggac agcctacgca atatgtatcg gcttgaaccc tcgtgccaag aaagagaaag
     2041 cagagaaaag taaatataat taatgttaga gttattagtt ttttttctaa aagggatcct
     2101 ccatccattt tctccattct ccttgtttaa ttatttatgc aatttatatc accaattaca
     2161 ggtaatttca agtattgtta taaatttaat ctgtagcgcc cgttccccga acgggcctag
     2221 tactgatacg gggatcagtc cctacatttt gttaatttat ggcgatctaa agatcgccgc
     2281 tacatatttg ataaacaagg ggattgcttc ggtgttttac ccctcgcaat gacaggcccg
     2341 agagtgtcaa tttatcaagg ataggcggga ctttccagtc ccgccatggt attgaagatt
     2401 aatttcatgc aattacaggg gatagccgag gttttctaac cctggataaa ggaatatgtc
     2461 atggatcccc gataaataca ttcggggatg acagacggga ggattcgggc agtatggagt
     2521 gtccgttccc cgaacgggca tgaatactag gactgatacg gggatcagtc cctacgggag
     2581 tcagcgctta catatttaat tagttaaata gtcattcgta aatcgtggat cgtgaatcgt
     2641 attgccaatc catgtaaaca aatcacgagc cacgaagcac gaaccgctga ttgacaacaa
     2701 tacatcactt aaatatattt aactccataa atcgccaagg ttttaaatct cggaggaaaa
     2761 tttgaggatt ttaattaccg atggccttgc cgaagagggt ctaaacctgc ttaaatccca
     2821 gccagagctt gaagttgtaa tgaagaaagg ccttccaaag gaagaaatca aggggatcat
     2881 aaaggactac gacgccctta tcgtaagaag cgggactaag gttacagcgg acattataga
     2941 ggcagcggga gaaagattaa aggttatagg gcgcgcagga atcggcgtgg ataatgtaga
     3001 tgtagcagcc gctacgagaa acggcacagt tgtgatgaat acccctgagg caaacgccat
     3061 aacgacggcc gagcatacga ttgcacttat gttttcttta gttagaaaaa ttcctcaagc
     3121 tcatttgtcg gtaaaatcag ggctttggga gagggaaaaa ttcaagggaa aagaaatctt
     3181 tggaaaaacc cttgggatta ttggacttgg aaatattggg aggttggttg cagagagggc
     3241 gataggacta aagatgaagg tgatcgcgta tgatcccttt ctcactcagg aggccgcagt
     3301 caagatgggc gtagagttgg tttcttttaa tgagttactt gggatggctg atataattac
     3361 aatccacacc cctctcacac ctgagacaaa gaatctcatt agaaaagaaa cctttctgcg
     3421 aatgaaaaag ggggtaatca taattaactg cgcaaggggt ggtatagtaa atgagctaga
     3481 cctctatgag gctgtaaagt ctggaatagt gggtgaggcg gcgcttgatg tttttgagaa
     3541 agaacctccc ggcaaggaca atccgcttct taagcttgat gagttagtct tcacgcctca
     3601 ccttggcgct tctacagagg aggcgcagac caaggtctct gtagcgattg cggagcagat
     3661 cgttgatttt tttataaatg gcgctgtaag aaacgctgta aatatgcctt cgataagcat
     3721 tgaactatta aagataatga agccctacat agtacttgca gagaagcttg ggagccttca
     3781 gggacagctt tgccgaagtg gcgttgaaga gataaatatt gaatatagtg gcgaggtcgc
     3841 aggttttgac gttgctcctc ttactattgc tgcactcaag ggttttctta cgccaattat
     3901 ggatattaca gtaaattatg tgaatgcacc attcgttgct agggagagag gaataagggt
     3961 aatagagtcg aagtcatcaa ggtctgagga ttttacgagt ttgatttcta taaaggtgaa
     4021 gacaaatata gatgaaaagc acgtttcagg gactatattc ggacgagaag agccaaggtt
     4081 tgtgagggtt aatggcttct cactcgacgt catcccgcag ggttatctcc ttataagtga
     4141 aaattacgat aagcctggat tcatcggggc tatgggatcg cttctgggaa aaaggggcgt
     4201 gaacatcgcc cgtttgcacc ttgggcgtga aagtgtaggt ggaagggcga tagcttttat
     4261 aaatgtggat tcacctattc cctcaggggt tatggaggaa ataagcgcgc ttccaaatat
     4321 aatatctgtt acacaggtag tgctttagat gcttgagcgt tttagcctac ctcaaggggt
     4381 aagggacttt ccccctgagg gggcagaaga gattagaagg gtcgaagaat gtctactgga
     4441 tgagttcaca cgctggggat atagaagggt aattacaccc tttttcgaat cccttgagac
     4501 tatttcagtt gggcttggcg atgatctaaa ggataagatt ttaaagtttg ttgatccgtc
     4561 aacgggcgat gttgtagcac tgaggccaga tataacacca caggtaggga gaatagttgc
     4621 gactcagctt agagatcaca tggggcctct aaggctctgt tacaatggaa gggtaattag
     4681 gtatgaagaa aggggtagtg gaaaggaaag agagatcttt caggtaggat gtgagctaat
     4741 ggggctttca tctcctgaag gggacgggga gattatagcg cttgcggtta aatcgcttga
     4801 aaaactggga ataaagaacc tcgttctaga tataggacat actggtcttg tgagattcct
     4861 tcttggaaaa actggaaatg taaggaatga aatagaggaa gcacttaaaa aaaaagacca
     4921 ggaggcgctt gaaaaggcaa cctcaaaggc ccctattcct caggcaataa agagggttat
     4981 attatctctt cctgagcttt acgggaataa agacattctt cgtagggcta aaaagatcag
     5041 cattataaga aaatatgtaa aggaattaga agacgtatta attttacttg acgaatttca
     5101 agttggatgt gagattaacg tagacttagg ggaactcagg ggatttaact attatacagg
     5161 tgtatctttt gagcttatca cgccacgcgt accgtcacct atgattattg gcggaaggta
     5221 tgatgaactc atagaaaaat acgggtataa atgccctgca acaggttttg ccgttgatat
     5281 agaagctcta ctagatgttg ctactagtac ctcctatgag gataataaga atcattttgt
     5341 tgtgatccca actaaaccaa gtctcaggcg tgaatcaatt aagatagccg aatcactaag
     5401 aaggagcggt tttaaagtaa tacttgatct taaagcagat gtgaacctag tgagagagag
     5461 aattaaggag aaccggagcc taagtagcta tggagtgatc ttgttagaaa cctttgggaa
     5521 gttacgatta attgagtcta gaacaggagt aagtaaggag ttttcaaatc tagaggagct
     5581 tctcagcagt atttgaaact gaaagtcaaa tgtccaataa attgcatgca tcagaaggag
     5641 ataaagcagt agcttctaga ctgtatttta acatgataat tctctttggc ttgctagagg
     5701 attttcactg tcattgcgag cgacgcggtt ccctaaatgc ccttcctctg gatagctacc
     5761 ccagaccctg cgaagcaatc tctcttttgg gtttgcttcg tcgctccctt ttatttccat
     5821 ttctcgcaca ctgcgctcga aataattctc ctcgcaatga cattccgtgc caacatagct
     5881 ttgctttggg ctttggctca cttgcttcat gcagagaaaa cggactatct gtcccatgcc
     5941 gataccatgc agcgtgcggt gggttcttca ttgttaggcg ggcattagat ggtagtaaac
     6001 attttccaag gaacgtgagt ataaaaaggg gataattaaa tttgccaaat atagttgtag
     6061 ttggtgcaca gtggggagat gagggcaagg gaagaatcgt tgaccttatc gcagaaaggg
     6121 ttgatgttgt tacaaggtac cagggtggta gcaatgcggg acacacaatt gtaacaaacg
     6181 ataagaccct ggttctccac catgtcccct ctgggatcct tcgtgagggc aaactctctg
     6241 tgatagggaa cggtgtagtt gtagacccta aggttttact tgaggagata gagaagatta
     6301 aatcgcatgg atataaggta gacgaagaga acttaaaaat aagcggcagc gcacatattg
     6361 ttatgccata tcacaaggtg attgaccttg cgcgtgagga gagaatgggc tctggtaaga
     6421 tagggacaac cggaagagga ataggtcctg tgtatgagga taaggcggca agaagggggg
     6481 taagattttc cgatctcatt tatcctgagg gcttttcgct aaggctccgt tccatactcg
     6541 aagaaagaaa cctatatatc acaagggttc ttggaagaaa tcccctcgaa tttgagaaaa
     6601 tctacgaaga atatgttatc tatggcaaaa agcttagaat gttcattaca gataccccaa
     6661 aggttcttaa cgacgcaata tctcagggaa agagtgttct ctttgagggg gcacaggggg
     6721 cacttcttga cgtggatttt ggtacttacc cttatgttac atcatcaagc gccggtgccg
     6781 gtggggcatg cataggtaca ggggtgagtc ccacgaagat agatattgtt atgggtgtgg
     6841 ctaaggctta cacaactagg gttggagagg ggccatttcc aactgaggtc ttaggggatt
     6901 tgggtatcaa gctaagagaa cttggtaatg agtacggctc gactacagga agacctagga
     6961 gatgtggatg gtttgattcc gttgcattaa ggtattcatc catggtaaat ggagtaacgg
     7021 gactcgccat tacaaagctc gatgttcttt ctagcttaga taagataaaa atctgtgttg
     7081 ggtatagatt taaaggtgaa ttactcactg aatttccgtc aaacacggag gttcttaaaa
     7141 actgtgaacc agtttatgag gagttggatg gatggagaga ggaattacgt gacttaaaaa
     7201 atctttcttc tctaccacct tcggcacgcg cttacttaga aaggatcgag gagacaacag
     7261 gcgttccgat atggattctt tccctcgggc catctaggga gaaggtaatt tttctgaggg
     7321 atattttttc ttaaaacggg ttttatagta gggtcgaaca cctattcgtc catttagttt
     7381 ttgtttttct taacaaaagc ttaatatttc cttcatatct aattaatttt tcttcaatat
     7441 tctttctctg aaaattctta caaaggaggg atgaagtgat gagattattt ctcggagcag
     7501 gattggctat tgctatgctt ctgacactgc ctttcgccaa ggactctagg gcgcaaagcg
     7561 cgagtgagca gataaaagca ctcaaaaagc aacttgagga aatccagaaa cagaaccaac
     7621 agcaaataga agagcttcag cagaagattc aagatttaga ggttaaaaaa gaggctgaga
     7681 aggaaaagat aatagaggag ataaaagccg aacaaaagga tgcctggtac aacaaaatag
     7741 aagttagtta caaaaaacca ggtgatggct ttactattaa aacaaaggat ggaaactttt
     7801 cgttgaatat gagactacgc gctcagtttc aattctctgt gaatgatacg actgatgagc
     7861 tcacagccac ggacttcagg ataagaagac tgagattcta ctggattgga aacgctttta
     7921 gaccatggtt cgaatattat attcaagtaa gtgccgacaa tggatccgat ctacagatta
     7981 gagatgcgta ttttaacgct gcttatgaga aggtagcaat tccaagagtg ggtcagttta
     8041 aggttccatt caatagagag gagctgaact cgtcatctga gctccagctt gtggaaaggt
     8101 ccattgtcaa cgaggagttt agcttaggac gtgacatagg tcctgcgcta tatggacttt
     8161 taggaaatta tgtcacgtac ggtgttggga tatttgatgg taatggaaga aatgatgtca
     8221 gcactgattc taatcttctt tatgctggaa ggataatgct cactccttgc tgtggtgaat
     8281 tgaagtatgc caatagctct ttcccaatag gcggggatta caagatagag cctaactttg
     8341 gggaggacaa gcctcttatt gcattcggtg tggcagcagc aggtatggaa ggtcttaaca
     8401 tagatataaa gactcctgat gcttctgtag acgatagatt cgaagctata ggcataacag
     8461 taggagattt tgttcaatta acggctgatg ctaacttcaa atacaagatt ttcagcatag
     8521 agggagaata cgacgccagg tggatctctc cagatgcaaa tcagggggtg agcactgaca
     8581 cagtatttga ccagggattt aggttacaaa gcggtgtctt tttgattcca aggcttgtag
     8641 aggtagcagg aaggttttca tatattgatt ttgatgcgag tgttccgaat atacagggtg
     8701 ttgatgaacc agacaacagt tggcagatca cacccggtct taatttctac atgtctcaca
     8761 gtcacaagtg gaagattcag cttgattatt ctttcatcaa aaatgagttt acgaacgctg
     8821 atgatgttga cgaaaacata ttcagggcac agcttcaggc ttatttttaa cccagtttga
     8881 ttagtctcct tacccttcct tggttgttgc cataagaaac ccgacacaca tccactgtgt
     8941 cgggtttctt gctttcttga gaataggtca ttagagttcg atttgtttgt aaaatagttt
     9001 atgaatgctg tctgctcaca aaaagtatgc ggtatcaaat aatgcttacg ttaaggtaaa
     9061 gtaaggcaaa ttttttgaaa gatcgggttg atttagatat acttatgcga cttgagtttt
     9121 cgtgaaatgg tgtatgtctt aaggctttag gagatatgtc tttagaaaaa ctaacagaag
     9181 gatgggtttt agcgctacaa ctctacgggt cagctaaaac tgtgagcttt tcagatcata
     9241 acttcacaaa cggcagtctg acctcgctca caggaggccg tgagagaaac acctttataa
     9301 gcagggaatt cgggtttaga gggtcctggc ctaatggatg gatgatagat aataagctga
     9361 atgaattgta caaaagacag tttggattca gagataaggt tgtaattcca attgtcgtaa
     9421 ttccccaatc tctctacggt ggttttagac caagtgttac agtggctatg gatgattctg
     9481 taggtattga tatcaatagc tatattgaaa acaaccttct ggtatctaag gatgcattgg
     9541 ttgaaataat aaagagtatt ggtgttgaca ttgcatcaat actacagttt cattacctta
     9601 gtctttacgg cgtatcttct gaggaaagga tatttcagat acaaaaagtg gccctcacta
     9661 acaataaggc ttatatagtg acagcttctc agataccaga gtctgaactt aaaacaaata
     9721 agagattgag gggcgattta gacaaaataa tgacttcttt ctcaattgtt agcttttaat
     9781 tcttgttttt ctttttcaac cctgatggct tcgatttcat tggcaaaata tatgcgttct
     9841 ttcccaagtt ctctcctgta tattcccata acctcaacaa tatctccctc agcaataggg
     9901 atgtgaccga gtacgaaaat atttacgaaa ttcccgtctt gatcggctag cttaaactca
     9961 gtaaacggtg tttctttatc ctctcgttca aatttatcaa acactaaatc ccaaatctta
    10021 ccctcaacta ttacccctgc gctatcaaag gcgattgggt tttctaaaat agctgctata
    10081 tcaacctttt tagcaggaat ggtggtatta ggatcctgcc actcacgggc acatccaagg
    10141 aaaaccaaaa gaattgaaga tgtaagcaaa aaggcctctc ttttcatcca atgcctccaa
    10201 cagagtgaac gttagtttaa gataaaactt tactacttgg ccgttaaggc accaaggcac
    10261 aaagaaatta tcgttggttt ttaatcacta gtcattctac agaaatctaa agactaaata
    10321 atccttagtg tctttgtggc taaatagtta tataaaacag tattaacaaa ttataacact
    10381 atattgcacg ctatggtata ttcaaatata tttttgaatg aaaattcctg acggagagtg
    10441 atcaaggaaa tgtctataaa gatctataat tcagagacga aggaactctt gggcgagatt
    10501 aaaagctact caatttcacg tttttatgtt acgggcatag atataatttg gggaatctgg
    10561 gttgatgccc cctttacaga ccctaacgag cagaaagcag ccttcacact acaggcaaca
    10621 aatgataatg gaaaagagat tacttttgag gatggatggt taaaggatgt tactggaacc
    10681 cgtccaaata tagcggtaag gattacagcc aagaaaaaag agtagttaaa ttataaatgc
    10741 aattcaatct ttttcttttc tttcgtatgc atctattatc tgtcttacaa gtgggtgcct
    10801 tacaacgtcc cttcttgaga aatggacaat tactatacct tcgatatctt taagcaatga
    10861 ttcagcttca agaagtcctg atttttcgtt acctccaaga tcaacctgag taacatcacc
    10921 tgtaattaca gcctttgagc taaacccaag ccttgtgaga aacatcttca tctgcatagc
    10981 cgttgtgttc tgtgcttcgt caagaatcac aaaggcatcg ttaagcgttc tccctctcat
    11041 ataagcaaga ggagctattt ctatagcacc cttttcaata agcctagtag ccttttcaaa
    11101 atccaacata tcgtatagcg catcaaaaag tggtctaagg tatgggtcga ttttctgata
    11161 aaggtcgccg ggcaaaaacc ccaacttttc ccctgcctcc actgcgggcc tcgttaggat
    11221 tattctgttt acttcacggc ttataaaagc agaaacagcc atagccattg caagataggt
    11281 tttgccagtt cctgcagggc ctataccaaa gacaatgtcg ttctttcgga tggcatcgat
    11341 gtagagcttt tgtgcaaggc tctttggtgc gattaatctt tttcttgtgg aaatacatac
    11401 agtatctagg aagatatctt caagaggggt tttatcttca attacaaccc ttgctgcgag
    11461 ttcgatatcg cctggatcga gagaataacc ctttgaaatg agacggtaaa tctcattgag
    11521 gaatcgctct acagattcaa ccacttcagg atctcccttg aggctcagct ttcctcctct
    11581 cgagtggatt ttgacgccga agacgctttc aatctctttg aggtttacgt tattctcccc
    11641 ataaagctgc ttgagaacac cattatcctc aaagttaagc tccctcgtta tgccattttc
    11701 agccattaat gtttcttgca tttagtttct ggagtccctc ctgttaattt gattttaaga
    11761 caagcacctc tttcttttgg ataattatta aggaacgtaa aaggtataaa tgtaatttgc
    11821 atccttggtt tagaaggcgg ctatatgaga agaatcttct acctaaaacc gtatattttt
    11881 tgaatcaata cttattatag ttcttcaaat ttagcctatt tattaattaa aattataaca
    11941 aaatacaaga taaaactcaa attttaaaga tttaacatga tttagtttaa tctcgttaaa
    12001 tctcgcatcc aattagagag attcaaagcc ggattaatgc taacaagtag agattaattt
    12061 caaaagtagg ttaacctact gttaacatta tatatttcgt gaatcggaga ccttataata
    12121 tcttgagtcg gatgacgagt catgagtcac gaaactcgat ctaacccctt atagaagact
    12181 ttgaccttct aggtttaata aaaccaggtc tgtttataag ataccctgcc cattttgcgg
    12241 cactgctttg ataagtaaat ccttaatctc atcgtaattg ccgttgcctc ttaataggcc
    12301 tttaaggtca tatgcataag gcgaaaagag acatgtcata attctcccat cagcagtaag
    12361 tctgattcga ttgcattttc cgcaaaatgg ctggcataca ttagatacaa cgcatatcgc
    12421 atctgtctgt taccgatagc cttagctttg ttactactat tccgaattta tctattagct
    12481 gagtcatatg attctgcatg gtagttaaaa catcgtgata ttgtatcagc ccgaaaggta
    12541 ccaagcaatc tcaatgggct tagttattaa atttttctta acttattttg aacctttact
    12601 tgtttataaa cagcacgaag gtacgccagc tttaataatg cttatagcgg ttgtgggcga
    12661 ggctaattac attattcctt acttgtttta ttctcaaaga gcttcacata ttgaccatat
    12721 ccttcttttt ctaaatcctc ctttgaaata aaacgaagag aagcagaatt catacaataa
    12781 cgtaagccag ttggtttagg tccatcgtca aaaacatgtc caaggtggga atccgcattt
    12841 ttacttctta cttctgttct taccgtaaaa aagcttctat cttccttttc aataatattt
    12901 ccttctccga gtggcttagt aaagcttggc catccagttc cagaatcgaa tttgtcaagc
    12961 gaactaaaaa gaggctctcc agaaactata tcaacataga tcccatctct gtggttatcc
    13021 caatactcgt tagcaaaggg tctttctgta gcttcttcct gggtaacctt atattgtagc
    13081 ggtgttagtt tttttcgaag ctcttctttg gatggtttct tatattcttc attattgttt
    13141 tttgagactt ttttttccat ttcatccccc catatttttt ttaagtattg atcgcgaccc
    13201 gaattgtacc tatagaactt atatcttatt ggattctttt tatagtaatc ttgatgataa
    13261 tcttctgctt tataaaatgc tgaagccatg ataatctctg tcacgatcgg ttttccaaat
    13321 ttcccggttt tatccagctc ttcctttgat ttctccgcta aacttttctg ctcatcatta
    13381 tgataaaaaa tagctgatct atattgtgaa cccctatcaa tgaattgacc gttcggatca
    13441 gtagggtcta tttgtttcca aaatacatca agaagctcgg agtaggtaat tttagatgtg
    13501 tcatatataa cttgaacaga ttctaaatga tcggtccctc catatgagac ctcttcatat
    13561 gttgggttcg ctttcttgcc acctgtatag cctgatatta catcgataac accatctaac
    13621 ttttcaaaag gtggctccat acaccagaag cagcctccgg caaatgtagc tttttcagtt
    13681 ttattatctg catatatcct ggtctgcatt attagcaaca aaagaaatga aaatagtata
    13741 ataatcttta acatatttta cctcctttat tttaatattg tattagacat atctaaatac
    13801 gctattaagg tatttacttc tcgttttcca tcaaagcttt atctaaaatt gatgaaagta
    13861 aatcggttcc tatttcacca cttctattat aaagtacact tccgtttgca ccaattatag
    13921 tgacatgcgg tatatagttt cgatagaaac gctctctcaa ctcattctgc tcattggata
    13981 tttttgaatc caaatcaaca attatgaaat ctatgtttcc ctcgtatttc tcatatagtt
    14041 caactgtcct tttcgcctgt cgcttggagt tgtagcattc atggtgatag aagataatgt
    14101 atgctggttt tccctgctgg atcataccat cggtaatatt atctccaata attaggggtc
    14161 cgtcgtttgt gtcgcttgag tagtctaggt ttggatttaa ttttaggtta gttgcagcgt
    14221 tggctgatgt ttgaataaat gaaaatgtta aaggtaaaag taaagtcaaa attagtattt
    14281 tatacattac tgcctcctta gcttattagc aatcattaat gctttcggtt gagtgtcaat
    14341 tctgtctatt tagacgcttg gagagccaaa gagtttcaat aaagtgattt gaagagtcta
    14401 caaagggtga ggggagaaaa ttttgtcaga aatcacctaa aattgtctcg taagaatcgt
    14461 cgaatccgct tcttcatatc aggagggata ttttcgtagc ttaatgatcg ggtaaacttt
    14521 attgtctctt tgtatgagtt taaaaaggaa acacagtcag ggcataccat taaatgctcc
    14581 tcaaataaca gtgccgttct agggtctaac tcaccagtaa gatagtctaa aatcaaagcc
    14641 gtagcctgct tacatgtgtc aactttctta gggtttactc gccttaattg agccttcttt
    14701 gccatgatct acttaataac ccccgaaata aataatattt gacgttgaaa ctgacaattt
    14761 ggattcaaaa tagaaaccct gcttctgttt aaactgacta agtaggtgaa taaccaagtt
    14821 gtgcagtaaa ttttcccctc aagaataatc gggctcgatg aagccgggcc ttgaccgcct
    14881 ggactgtaat ccctaatgtc gcgctaatct caggattgga catctcttcc atatcgctca
    14941 tcactacaac agccttgtcc atcggggaaa gtttttccat ggcttctttt atgatttgac
    15001 tatattcttt aatagcaaca aacttatcaa cttcttgagt ccagtcaacc actggcctaa
    15061 ctaagtgatg accgtccttc ctaaacttag gcaaatattc gtctagcgac acctctttct
    15121 gcttcttacg ttgacggagc ttagtaagtg cagcattcgc tgtaaggcgg tagagccagg
    15181 ttgagaactc tgatttacct agaaatgaat tagccttcct atataccaaa agaaagacgt
    15241 cttgaactac atcttctgca tctgcttcat ttcctacaaa cttgaacgcc tggcggtaga
    15301 ccctgcttac ataatggttg aaaatcgctt caaatgcctt ttgatcgcca gccttcaccc
    15361 tctggataag caacctctca tcctcgcctg aaagctgacc ctttccactt aattcttttt
    15421 tcgtcactct gtgacgtcga ggaatcacag tttttattct ctccgaatca ggagtattac
    15481 ataattggtt gtagtatcga aagaaagtct tgtcttttac caagatggga atttatcaga
    15541 ttgttaaaga aaaaacaaat atgcagtctc ttgtaagcgg aggtattata caaatagact
    15601 ccggggagat aataggggaa atattatgat ggcaactatt caaccatgaa tcaaaataca
    15661 aatcccaaat cacaaatttc aaacaatttt cagtttctga attccgaaca tttgttattt
    15721 ggaaattgtg atttgtttgt aattttgaat ttggtaattg gagcttgttc actggtggat
    15781 aaatagttac taaggaggtg gagtaatgac atcacccaca ctaattgcca ctcttggatt
    15841 aggatttatt ctcggtttaa aacatgcact tgatgcggac catatcgtgg ctgtatcaac
    15901 aatcgtgagt gagcataaga gcatttctaa atcctctctt atcggtacat tctggggact
    15961 tgggcacacg gtatcgcttt ttcttatcgg tcttctggtg attttgctaa gactcgttat
    16021 cccacctaag ctcgcccttc taatggagtt tggggttgcg atgatgcttg ttttcttagg
    16081 tgctaatatt atctggaagt tctttagaga aagaaaaatt catatacact cgcatgaaca
    16141 tgatggagta ccacatgctc atattcacct tcatgaacag ggtagagagg aagcccatga
    16201 gcataatcac ttaatcaagt ttggcagtaa accattcctt gtaggactcg ttcatggtgt
    16261 tgcgggaagt gcagcactta tgcttttagt tttaacgact attccttctc ccttaactgg
    16321 gcttctctat atagtcgttt tcggagtagg ctcgattggt ggaatgcttt taatgagttc
    16381 attgatagga cttcccttcg ttttcactgc aaataagttt gcttctatga accaggcaat
    16441 aaaagtccta gctgggacct cgagcatagc atttggtgtc tatctcggat gggagatagg
    16501 tttcctgcaa tgcctttttc cctaaaaatc aaaactgctg tgtttacttt ctcatcgtat
    16561 aaaaagatat tataattgaa ctccctaaaa aattatcaat atgagagaag catagattgt
    16621 ccgctgcaaa ggcttgcctt ctgattgggc tttttattga gttatcaaca tccatttcat
    16681 ttgcctttcg tccctttaga acagaagatg cgggaatctc tccaatcgga ttttttgcgg
    16741 tggaattagg aaatgaattt aggaaaaccc atgatggtaa agataacgtt acaacttttg
    16801 ctctgacata tggtctattg gactggatag aaaccgaaat tgactttgcg cttctaacct
    16861 tgaggccaga ttctggtaaa aatgagtttg gatttggcga tactgtttta ttatctaagt
    16921 tcaaaatact tggtgaaaaa ggggttcttt atgatgatga tttgtttcct gacatggtaa
    16981 taaggccatc cgttttaatt cctacaggtg atgaggataa aggtacaggc tcaggtcaaa
    17041 tagaacttgg cattttgctt gttctggaaa agacgttttg gaaagtcgga tctagggcaa
    17101 atatcggtta tttagcatca aacgatccta ctttcgatga aaatttcgaa gataggtttt
    17161 tttatggagt ccaagccgat gtccctttgt ttactgagag attaaggttt ggttcagaga
    17221 ttacaggtca atttgcttca ggtgagggat ctccactatt ttcgctaacc gggttggttt
    17281 ttcaagtcac tgaaaacatt gttcttgatg gcggagtgga attagggctt aaaaatgcag
    17341 gctctgccgt aacagtcatt gttggcataa cctttggttt taacccattt gaggtgtttt
    17401 taactaatag ataaattatt tgctcaggta atacactatc aaagttgcac taaaattcta
    17461 aaatataacc ccatttgtag aggtgtggaa atcccactcg gttccacttt aatatgagga
    17521 aggataatcc tcccttaccc tcctttacca aaggagggga ataaaaaaat caccctttgg
    17581 gaagggggag agccatacga atttccccct ttcttaaagg gggatttaat aaaataatga
    17641 gcgtaaatcc atttaaatca cattgaatta gaacagtaca aattttttgt gttatcattt
    17701 tgttgctatg ttgaagggga gtaagttttt agtcaccttt tttctgcctt taatcttaac
    17761 tcttctattt actttttcag caattagttg ggctgagtat aatgatataa cagccgagga
    17821 gattattgag catatacgat tcttagcctc ggatcagctt gaaggaagaa aagctgggac
    17881 agtaggatgt gaaaaagccg ctgagtatat tgcatctcat tttaaggaag caggacttaa
    17941 accccttgga gataatggaa cgtatttcca gaatttttct tttacctctg gagtaaaact
    18001 cggggagtct aactcaatga ccgttgatat gggtgaagaa aagttggacc ttcaagttgg
    18061 caaagatttt ctacctttaa gtttttcaag tgatggagaa cttaggggtg agttggtgtt
    18121 cgcaggctat ggaataagct cgaagcagct aagctacgat gattacgcag gaatagatgt
    18181 aaaagacaaa atcgttttag tcttacgtta tacgccggac ggtaataatc gtaaaagccc
    18241 tttctataag tatgcatcgc ttcgctataa ggctattaac gcaagggaga aaggagcaaa
    18301 aggcattata tttacaacac ctctttctca gaaggaagaa gagggtttag gtggcataag
    18361 attcgatcgt tctttcaccg attccggtgt tcatgcaata attctaagaa gggagatagc
    18421 tcaagaggtt ttgaagaccg ctgccaagga tatgaaaacc ttggaacaga agctaggtga
    18481 aaagaaacct tcttcctttg ctattcctga aactgaagcc cagattcata ccgaacttat
    18541 ccgtgaaaaa agccctactg caaacgttat aggttacatc gagggttctg atcaatcact
    18601 caaagatcag gtgataatca t
//
LOCUS       DATDRL010000342         2417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_815311, whole genome shotgun sequence.
ACCESSION   DATDRL010000342 DATDRL010000000
VERSION     DATDRL010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815311"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..1896
                     /gene="ltrA"
                     /locus_tag="VI935_11450"
     CDS             382..1896
                     /gene="ltrA"
                     /locus_tag="VI935_11450"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HLE26242.1"
                     /translation="MVEDGTRRRWNIISRDDCGTEGETAVDRALIVPMKCRSINTWRE
                     GGQENKYEKYREFSEMSFDSVDKTKQETEAAHSRPREDKWNWVERTIWTDRMLMALDN
                     GVKGNKWFSLIDKVYKLATLKQAWQNAKANKGAAGIDQITIDRFSRNASKYLEEIATE
                     ITQGRFKPSQIRRTYIPKSNGKKRPLGIPTVKDRIVQGAIKMVLEPIWEREFLDTSHG
                     FRPKRGAYGAIQEVSRHIGEGYTFVVDADIEGYYDNIPQDRLMQIVATKIADVRILRL
                     VEGFLTAGIVEEMKEWIPTKGTPQGSVLSPLLANIYLHPLDVLMKQRGYRMVRYADDF
                     MILCKSEQEAQKAKEEVQQWMTENGLKLHQGKTRICNCAIEGQGFDFLGFRFDQKYKF
                     VRKSSKEKLKEKIREKTKRTAGKRVSEVIKSLNKILIGWFNYFKIAQGKIHRETDQFI
                     RRRLRAILRKQEKRGGMGYTRDDCDRWPNEYFAKLGLFSLEAARAQWRMANQSR"
BASE COUNT          870 a          404 c          596 g          547 t
ORIGIN      
        1 tgggctgtct cagagtcaga gcaactcgct tgttaaagac agatcggata taaacgagat
       61 tgaagatgtt gtagggaaac tgagagcttc aaaaagttta tgtccttgct gttagaaccc
      121 cctggattta tccatgggga gtaagtgcga ctgtgaaagt attccaacaa tgaggtgaaa
      181 gtccttatca atgtaatcta caacattgta gtcgaaagta actgcgtcat cgtgagatgg
      241 ggtggaaagc aactggagac gaaaactcgg tctgtaggat aacgaacctg attcggcggg
      301 gatgaacggc cagcctccat actcgggtga agcctataaa gctgttacac aagtagagaa
      361 agtagtaaca ggttcataaa cgtggttgag gacggaacga gaagaaggtg gaatatcata
      421 agcagggatg actgcggaac tgaaggagaa accgcagtag acagagccct catagtacca
      481 atgaaatgta ggagcataaa tacatggagg gaaggggggc aggaaaataa atacgagaag
      541 tacagagagt tttcagaaat gagtttcgat agtgtcgata agactaagca agaaacggaa
      601 gcagcccata gtagaccccg agaggataag tggaactggg tggaacgaac tatatggacg
      661 gatcggatgc tgatggctct ggacaacggg gtcaaaggaa acaaatggtt cagcttaata
      721 gacaaggtct ataaactagc aactctcaaa caggcctggc aaaacgctaa agccaataag
      781 ggtgctgccg gaatagatca aatcacaatc gacagattca gccggaacgc aagcaagtat
      841 ctagaagaaa tagcaacgga aataacccaa gggagattca aaccatcgca aattaggaga
      901 acatatatcc cgaagagcaa tggcaagaag cgcccactcg gcataccaac tgtcaaagat
      961 cgaattgtgc agggagccat caaaatggtt ctagaaccta tatgggaaag agagtttctc
     1021 gataccagcc atgggttcag gccaaaaaga ggagcctacg gtgcgataca ggaggtaagt
     1081 cggcatattg gagagggata cacatttgta gtggatgcgg atattgaagg atattatgat
     1141 aatattcctc aagatcgact catgcaaata gtagcaacaa agatagcaga cgtccgaatc
     1201 cttcgacttg tcgaaggatt tctcacagca gggatcgtag aagaaatgaa ggaatggata
     1261 ccaacaaagg gaactcctca agggagtgtt ctcagtcctt tattggcaaa tatatacctg
     1321 catccactcg acgtgctaat gaaacagcga ggttacagga tggtgagata cgcagatgat
     1381 tttatgatat tatgcaaaag tgaacaagaa gcacaaaagg ccaaagaaga agtccagcaa
     1441 tggatgacgg aaaatgggct taaactgcat caaggtaaaa cgagaatatg taactgtgcg
     1501 atcgagggac aaggatttga ctttcttggt tttcgatttg atcagaaata taaattcgta
     1561 cgcaaaagca gcaaagagaa gctgaaagaa aagatccgag agaagacaaa gcgcaccgca
     1621 gggaaacgag taagcgaggt tatcaaatca ttaaataaaa tactgattgg atggttcaac
     1681 tactttaaga ttgcccaagg taaaatacat cgagaaacgg atcaattcat taggcgaagg
     1741 ctgagagcta ttttgcggaa acaagaaaag cggggcggaa tgggatatac aagagacgat
     1801 tgtgatagat ggccaaatga atacttcgct aaacttgggt tgttctccct agaagcagct
     1861 cgagctcaat ggagaatggc gaatcagtcc cgatgaggaa attattaact ggagagccgt
     1921 atgcaggaaa actgcccgta cggttcggag ggaggggaga tcatatcttc cctaccccta
     1981 tctcaggaag tttattaaat ggtataaaga ctcaataaaa agccaatctg ttttactcag
     2041 agctgcgatt gctcatttat attttgaaat catacatccc tttgaagatg gtaatggccg
     2101 tattggtagg gcattggtag aaaaagccct ttcacaaaat ttgaaaaagc caagtttaat
     2161 tgctatttca aagtaatagc aaaaagaaaa agagaatatt ataatgcttt agctgaatgt
     2221 aataatagtt taaatattaa caactggatt aaattttttg ctgaaatagt aatgcaagct
     2281 caaaaagaat cacttaaaat gataaatttt ttaatgacaa aatctaaact aataaatgct
     2341 ttaaaagata atattaatga acgccaggaa aaagttttgc ttagaatgtt tgcagaagga
     2401 ttaaaaggct ttactgg
//
LOCUS       DATDRL010000343        11630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_399133, whole genome shotgun sequence.
ACCESSION   DATDRL010000343 DATDRL010000000
VERSION     DATDRL010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11630
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399133"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="VI935_11455"
     CDS             complement(<1..151)
                     /locus_tag="VI935_11455"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459515.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HLE26243.1"
                     /translation="MSYVKGLRCRECGKEYPKAPIHVCEFCFGPLEVTYEYDEIKRVF
                     SRDVIK"
     gene            553..834
                     /locus_tag="VI935_11460"
     CDS             553..834
                     /locus_tag="VI935_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HLE26244.1"
                     /translation="MKAVWKNVVLAESKDTLVVEGNHYFPPESIRKEYFRKSDTHTTC
                     SWKGVASYYDIVIANDVNKDAAWYYHEPMGAAKDIKNYIAFWKGVEVVK"
     gene            complement(1114..1719)
                     /locus_tag="VI935_11465"
     CDS             complement(1114..1719)
                     /locus_tag="VI935_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HLE26245.1"
                     /translation="MASQYIQFWFEFASTYSYLSVMRIERLVGDTGIPIEWKPFLLGP
                     IFHSQGWNTSPFNIYHAKGRYMWRDIERLCNKYNIPFRKPTEFPRNGLLAARVACIAA
                     SEGWCPEFTQKVFKANFAEDQDISDSTIISEILESIGKDSSHEITRAQSPENKELLRK
                     QTEEAINLGIFGAPTFVVGTELFWGNDRLENAIDWYKKYIV"
     gene            complement(1800..2429)
                     /locus_tag="VI935_11470"
     CDS             complement(1800..2429)
                     /locus_tag="VI935_11470"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007102637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLE26246.1"
                     /translation="MDNKSHWENIYKTKASTQVSWYQEHLQTQLRLIERTGVEKTAQI
                     IDVGGGASTLVDDLLERGFKHITVLDISSAAIDAARKRLGSRREEVTWIEADITKISL
                     PYDYYDLWHDRAVFHFLTSAEDRQRYVEAVKYSLKPRGHLIISTFALEGPPKCSGLNV
                     VRYSPKSLHEEFGNEFELVDSASEVHLTPFGTNQKFIYCHFKKALKDRE"
     gene            complement(2589..2870)
                     /locus_tag="VI935_11475"
     CDS             complement(2589..2870)
                     /locus_tag="VI935_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26247.1"
                     /translation="MTDIDIAEKLEELLRKTGSEHHKAFIKTKGEDPEWPLWYADYLL
                     EKLAKLLNAKFTKSELIYLLVMVEKERALMAPGADWPRYYANFFIERYT"
     gene            complement(3235..4803)
                     /locus_tag="VI935_11480"
     CDS             complement(3235..4803)
                     /locus_tag="VI935_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HLE26248.1"
                     /translation="MTCRLISYCRGIACYAPTCSLSMVLFRTGLILSLLFITDTTGTQ
                     PATKVKVGSKAFTESVILGEIVAQLARTQEPNVKHLKGLGGTRVLWSALLKGEIDIYP
                     EYTGTITQEILAGKNIGDKETLKKVLAEKNILMSHPLGFNNTYAIGMREDVAERLGIT
                     KISDLRKYPELKFGFTNEFMNRGDGWPSLRRAYNLPQKNVRGLDHDLAYRGLESGSID
                     VIDLYSTDAEIRYYKLRVLDDDLHHFPEYNAVLLYRSDLKKSAPDVLKTILKLEGRIP
                     EKTMIDMNAEVKLDGIPESVVASEFLKKNLSITTEPKEETFLTRLVHNTLQHLFLVAI
                     SLAAAILISIPLGILSHRKEKIGQAILGIVGIIQTIPSLALLVFMIPLLGIGSAPAIV
                     ALFLYSLLPIVRNTYTGLQDIPVGISESAEALGLPTGARLRLVEIPMASRSILAGIKT
                     SAVINVGTATLGALIGAGGYGQPILTGIRLDNVGLILEGAIPAAVLALLVQGLFDLLE
                     LVAVPKGLRLKSEA"
     gene            complement(4808..5551)
                     /locus_tag="VI935_11485"
     CDS             complement(4808..5551)
                     /locus_tag="VI935_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLE26249.1"
                     /translation="MIKLENLSKTYASTRALDSINLTIHPHKTTVLIGPSGCGKSTLI
                     RLIIGLIKPDKGNVYVKDEMLTPINLFTIRRSMGYVIQDGGLFPHLTARENVFLMAKY
                     LGWDNDRSGKRIVELCELTKFPVDALDRYPVQISGGQRQRVSLMRALMLDPDILLLDE
                     PLGALDPLIRFELQNDLKEIFHTLGKTVVMVTHDIGEAGFFGDSIVFIRDGHIVQQGT
                     LMDFVRTPTDPFVTSFINAQRSPLESLGE"
     gene            complement(5796..6296)
                     /locus_tag="VI935_11490"
     CDS             complement(5796..6296)
                     /locus_tag="VI935_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019502179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HLE26250.1"
                     /translation="MIIKPKRIEPEPGQESVWDYPRPPRVEDTTKHIKVIFNNVVIAD
                     TRNAKRVLETSGAPVYYLPPKEIKTEYLTITRKRTVCEWKGVASYYTIIVGGKKAANA
                     AWAYPDPTPGYESIKDHIAFYPQKMDACYVDGERVIPQPGIYYGGWITSDIVGPFKGE
                     PGTEEW"
     gene            complement(6389..7351)
                     /gene="egtD"
                     /locus_tag="VI935_11495"
     CDS             complement(6389..7351)
                     /gene="egtD"
                     /locus_tag="VI935_11495"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HLE26251.1"
                     /translation="MNNNLRTLQKYEPEEEILLAEVLEGLQKPQKHLPCKLFYDERGS
                     GLFDQICELEEYYLTRTEMEIMNKYIEEISACLSKKCLLIELGSGSSKKIRLLLDHLE
                     EPAGYVPVDISAEHLMKSVTTLAKDYPQLKIVPVYADYTQRFDLPYLNLPWSHKVIYY
                     PGSSIGNFTPKEATTFLTRIARIADKTGGLLIGVDLKKEKSVLEAAYNDKNGITAEFN
                     LNILRRLNLELGTDFELDQWSHYAFSNSDEERIEMHLISLRNQFVHVDNIEFFFKKGE
                     SILTEYSYKYTLEKFEQLVSDVFKVKHVWTDDECKFSIQYLSVR"
     gene            complement(7344..8657)
                     /gene="egtB"
                     /locus_tag="VI935_11500"
     CDS             complement(7344..8657)
                     /gene="egtB"
                     /locus_tag="VI935_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HLE26252.1"
                     /translation="MNTKKTITKVSEKDQSESINSSLVKRYQIVRELTETLCKPLATE
                     DYVIQSMPDVSPTKWHLAHTSWFFETFILNKVNKHYKSLHPQYNYLFNSYYVQVGKRH
                     FRPERGLLSRPTVEEVYSYRHHVDQHMLEFLEKANQAHMEEIAPVIEIGLNHEQQHQE
                     LILTDIKHVFSVNPLRPVYLHIKHDSPRPFTIPEMRWVRFPEAAYWIGHDGNGFAFDN
                     ESPRHKVYLNSFKLAYRLVTNREYLAFIEDGSYERPELWLSDGWYTVEANDWKAPLYW
                     EKGDRHWWYFTLSGMREVDLDEPVTHVSYYEADAYARWKGVKLPSESEWEIASSKLPI
                     EGNFVESGNFHPRTLNQDSTNRGLLQMFGDVWEWTQSPYIPYPGFKTLPGALGEYNGK
                     FMSNQMVLRGGSCATSMSHIRKTYRNFFPPHARWQFMGIRLAKDE"
     gene            8798..9709
                     /locus_tag="VI935_11505"
     CDS             8798..9709
                     /locus_tag="VI935_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HLE26253.1"
                     /translation="MKNNKIHVVLFIITLGTTFLAGFLQGGNVTSGIAFSAALIFILG
                     AHEMGHYVYGKRYGVDITPPYFIPAPPVISPIGTFGAFIKIKSPIATKKALFDIGIAG
                     PLVGICAAIPVIIIGIKLSSIVDPSSREFEGGMTLGSPLIFSFLADTFFGKVPDGKDL
                     LLHPVAFAGWIGLFVTALNLIPAGQLDGGHIMYALFPRKWHRRASVSMIFFLLILGIG
                     TQPIIHLLQSLWKGIPLETYKDLLTYDGWLGWVMWAMLLSIMGTRHPPTMDDNISLDG
                     KRKFLGIVALLVFIGCFTPVPIGLPKS"
     gene            9764..10498
                     /locus_tag="VI935_11510"
     CDS             9764..10498
                     /locus_tag="VI935_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLE26254.1"
                     /translation="MSDKVGLITGGARGIGRAIALALAYQGWSIALCYRTSEKEAVKI
                     VNEIEQKGSRGLRVRCDVSDPEAAQAFVKHVENEWGHIDALINCAGPYHRVNLLEETI
                     EGWHSMFDNNLHPVFYLSRAVVPGMKERKWGRIINFSMANADQLVAQPEITAHYIAKA
                     GILILTRSLARVLAPYGITVNSISPGFISSGSAPDDELEKMAKRIPAGYVGGTQDAVT
                     VATFLLSEEAGYINGANIHLSGAWGI"
     gene            10828..>11630
                     /locus_tag="VI935_11515"
     CDS             10828..>11630
                     /locus_tag="VI935_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HLE26255.1"
                     /translation="MAKETLTITDNRTGKTYEIPINSGTIRAMDFRQIKISEDDFGIM
                     SYDPALGNTASCNSRITFIDGEKGVLLYRGYPIEQLADKSNYLEVAYLLLYGELPSKS
                     QYDAWVYNITHHTIFHENIKKFIDGFRYDAHPMGMLLSAVGALSTFYPEAKNIFDPDS
                     RKKQFHRLIAKMPTLAAFSYRQTLGLPYVYPDNDLSYTGNFLNMMFKMTELKYKPNPV
                     IEIAIDILFILHADHEQNCSTNAMRSVGSSLPDPYSATAAAIAALYGPL"
BASE COUNT         3222 a         2343 c         2360 g         3705 t
ORIGIN      
        1 tttttattac atcccgagaa aaaactcttt ttatttcatc atattcgtat gttacctcaa
       61 gtgggccaaa gcaaaactca cagacatgaa taggtgcctt tggatattcc ttcccgcact
      121 ctctgcatct tagtcctttt acataactca tttctttcct cttaaaatta gtctgaaaag
      181 aattaccctc tctttataat gttgtcaaga aatacggctg tatgtcctgt atgaaaccca
      241 cttatatttt tacctgtttt cattataata tctgggtgta agaaatggat tttgagtttc
      301 attatttaac ttgagaattc cctgtagctt gctacagggt ttccacaata gtgtattgtc
      361 attgcgaaca cgttcatttc gttcagtgca ggctccgtga agcaatctct tattttagat
      421 tgcttcgtcg cttttctcct cgcaatgaca gctataacgg tatattttta agaccatacc
      481 ctgcagcttg ctgcagggtt attgattgaa taatgttttt gtcttatagt aagataaaat
      541 gtggtaaaag tcatgaaggc tgtgtggaag aatgttgttt tagctgagag taaagacacg
      601 ttagtggttg aggggaacca ttattttccg ccggagagta tcagaaaaga atatttccgt
      661 aaaagtgata ctcatactac ctgttcatgg aaaggggtag ctagctatta tgacattgtg
      721 attgccaatg atgttaacaa ggatgctgcc tggtattatc acgaacctat gggtgccgct
      781 aaggatataa agaactacat tgcattttgg aagggtgtag aggtagttaa ataaagttag
      841 ttgctcttca ataaattccg gtctctagca tcatggttaa aaggaacccc acaaagaaca
      901 tagcagtagc aagagaagtg tctttttcag catgagcctc ggttagcagt tcttcggtca
      961 cgagataaag tggtgccgac atggcgaatg caagcacttc gttaaactca gggttcctcg
     1021 caatgacggt tataattatg cataatcaaa aatcgatttg gtatattcaa ttcaataggt
     1081 gtaactattt aaccactact tatagaaatg aatttacacg atatactttt tgtaccagtc
     1141 aatagcgttt tctaggcgat cgttacccca aaataactca gtgcctacta caaaggtcgg
     1201 agctccgaag attccgaggt taattgcttc ctctgtctgc tttcttagta actccttgtt
     1261 ctccggtgat tgagctcttg tgatctcatg agaactgtcc ttgccgattg attctagaat
     1321 ttcagaaatt atcgtagaat ctgaaatatc ttgatcttcc gcgaagtttg ccttgaacac
     1381 cttctgtgta aactcgggac accatccctc agatgctgct atgcatgcga ctcgtgctgc
     1441 tagaaggcca ttcctaggaa actccgttgg ttttctgaag ggaatattat atttgttgca
     1501 taaacgctca atatcacgcc acatgtaacg gcccttggca tgataaatat taaatggcga
     1561 ggtattccat ccctggctgt ggaatatcgg acctaaaaga aacggcttcc actcgattgg
     1621 tatccctgta tctccaacca agcgttcgat ccgcattaca gaaagatatg aataagtact
     1681 cgcaaactca aaccagaact ggatatattg tgaagccatt tctttttccc tgagaagact
     1741 aattcaactt ggaactaagc caccgcattg aacccaactt gcggtagtga cataacaggc
     1801 tactctctgt cttttaatgc ttttttgaaa tggcaataaa taaacttttg gtttgttcca
     1861 aaaggtgtca ggtgcacttc gcttgcacta tcaaccaatt caaattcatt accaaactcc
     1921 tcatgcaggc ttttaggact gtatcttacg acatttaacc cactgcattt aggaggacct
     1981 tcaagagcga aagtagaaat aattagatgc cctctgggtt tcaacgagta cttcactgct
     2041 tctacgtacc tttgacgatc ttcagcactg gtcaaaaaat gaaagaccgc acggtcgtgc
     2101 caaagatcgt aataatcgta gggaagagaa atttttgtga tgtcagcttc tatccatgtt
     2161 acttcttctc ttcgtgaacc tagccgcttt ctagctgcat ctattgctgc agatgaaata
     2221 tcaagaactg ttatgtgttt gaatcccctt tcgagcaggt catcaacgag agtcgaggca
     2281 ccaccaccta catcaattat ttgagcggtc ttttcaactc cagtccgttc aattaatcgt
     2341 aactgcgttt gcagatgctc ctgataccag ctcacttgag tcgaagcctt tgtcttgtag
     2401 atattttccc agtgcgattt gttatccacg attgtgtcat ttttgtgttt ggttttagaa
     2461 gtataaaaag gattagaact catgagaaca ttaaaagtat acgttgattg ggatatgtaa
     2521 tcaaaattaa atgcgattca taagttgagc aaagagaagt ttaattcctg tcactaaatc
     2581 ttagaaatct atgtataacg ctctataaag aaattggcat aatatcttgg ccagtcagcg
     2641 ccaggtgcca tgagtgccct ttctttttca accatgacga gcaaatagat taactcactc
     2701 ttggtgaact tggcattgag taattttgca agcttctcta acaagtagtc cgcataccat
     2761 agtggccatt caggatcttc tccttttgtc ttaatgaacg ccttatgatg ctcacttcca
     2821 gtttttcgaa gaagctcttc cagcttttct gcgatgtcaa tatcagtcat aatgttttct
     2881 ccttattttt attgctttat ttaggataat tacatcgttt gattcatttt caaaaactat
     2941 gctgagagta agtcatcgac aagctttttt ctctaaatct gagggtcaag aattttaggg
     3001 tcaggtcttt gattgtgaat tattgagctt aaaaacatgt atatctttga atattaaacc
     3061 agaatgccta gctatagaaa ttgaaaataa ttaaaacctt atatttcaag acctgaccct
     3121 aatttgcttc ttgcaaggaa gactttgtct ttatgtgttt tttacttttt ttatcttcct
     3181 tcctaaaggt agactacagt attggtaaag gtctagaggt agttaaatga accactaggc
     3241 ttctgacttt aatctcaatc ccttcggaac cgccacaagt tctaataaat caaacaaacc
     3301 ttgcaccaga agtgctagaa cggcggcggg gatagcgcct tcgagaatta gtccgacatt
     3361 atcaagtcgg attcctgtga gtatcggttg accgtagcca cctgcgccga taagtgctcc
     3421 aagtgttgct gtgcccacat tgataaccgc agaggttttg atgccagcta atatcgaacg
     3481 cgaagccatg ggtatttcta ccagtctcag gcgtgcgccc gtaggtaaac ccaatgcctc
     3541 tgccgattca ctaattccta cgggaatatc ttgaagtcct gtataagtat tcctgacaat
     3601 cggaagtaag ctataaagaa atagggctac aatagcaggc gctgagccaa ttccaagtag
     3661 cggaatcata aacacaagta gtgccaacga tggtatcgtt tggattattc caacaattcc
     3721 taggattgcc tgtccgattt tttctttcct gtgtgacaaa attcccaggg gaatcgagat
     3781 taatattgca gcagctagtg atatagcaac cagaaaaaga tgctgcaagg tgttatgaac
     3841 caatcgggtt agaaaggttt cttcttttgg ctctgtagtg atggatagat tttttttgag
     3901 gaactctgat gcaactacac tctccggtat tccatcaagc tttacctcag cattcatatc
     3961 aatcatagtt ttttctggaa tgcgtccttc caattttaaa atggttttca gtacatcagg
     4021 tgcacttttt ttaaggtctg aacggtaaag tagaactgcg ttgtattctg ggaagtgatg
     4081 caaatcgtca tctaataccc gtaatttata gtagcggatc tcggcatcag tcgaatatag
     4141 atcgatgacg tctatgctcc cactttcgag tcctcggtag gctaggtcat ggtctaatcc
     4201 ccttacgttt ttctgaggga gattataggc tctacgaagg ctaggccaac catcgccacg
     4261 attcataaat tcattggtaa atccaaactt cagctcagga tacttacgca ggtcggatat
     4321 ttttgtgatt ccaagccttt ctgcaacatc ctccctcata cctattgcgt aggtgttgtt
     4381 aaaccctaat ggatgactca ttaaaatatt cttttcagct agcacctttt taagtgtctc
     4441 tttgtcacct atattcttac ctgcaaggat ttcttgggta attgtcccag tatattctgg
     4501 gtatatgtca atttcccctt ttaatagcgc actccagagt acacgggttc caccaagccc
     4561 tttgagatgt ttaacattag gctcttgggt tctagcgagt tgtgctacta tttctccaag
     4621 aattacggat tcggtgaacg cctttgagcc tacttttact ttggttgcgg gctgggttcc
     4681 agtagtgtca gttatgaata aaagcgataa tattagcccc gttctgaaaa gcaccatgga
     4741 taaggaacac gtaggggcat agcatgctat gcccctacaa taagaaatca atcgacaagt
     4801 catgctacta ttctcctaga gattctaatg ggctacgctg tgcattaatg aaacttgtaa
     4861 cgaatggatc ggtaggtgtg cgaacaaaat ccattagtgt tccttgctga acaatatgtc
     4921 catctctaat gaaaacaatt gaatctccaa aaaaaccggc ttcaccaata tcatgtgtaa
     4981 ccattacaac agttttacca agtgtatgaa aaatctcttt tagatcattt tgaagttcaa
     5041 agcgaattaa ggggtcgagt gctccaagtg gttcgtcaag taaaagaata tcggggtcta
     5101 acataagcgc ccgcattaag ctaacgcgct gtctttgtcc cccagaaatt tgcactggat
     5161 aacgatcgag tgcatcaacg ggaaactttg ttaattcaca tagttcaact atacgcttcc
     5221 cgcttcggtc attatcccaa ccaaggtatt tagccattaa aaaaacattt tctcttgcag
     5281 taaggtgggg aaaaaggccg ccatcttgta ttacatagcc catacttcga cgaattgtaa
     5341 acaaattaat tggtgtaagc atttcatctt tcacatagac gttacccttg tcaggtttta
     5401 taaggcctat aatgaggcgt atcagcgtgg acttgccgca accgctagga ccgataagta
     5461 ccgtcgtctt atgagggtgg attgttaaat taatggaatc aagcgccctc gtagaggcat
     5521 atgttttaga gaggttttct agttttatca tgtagttata ataccaattt cttgttgaaa
     5581 tgttacaacg gctcttattt taccaattct atctcattgc gagcatagcg aagcaatctc
     5641 gctttttaag gtttcgttac cagttgagtc ttggaggatt gcttcgtcac tttgcccctc
     5701 gcaatgaaag gcaagggtat gatcctaagg taacaaagaa tattatggat gtttattttg
     5761 tctactataa gtttcaaaga ctaacgatct gaatattacc actcctctgt tccaggctct
     5821 cccttaaaag gtcccacaat gtcgcttgta atccagccac cgtaataaat tccaggttga
     5881 ggtattactc tttcaccatc aacatagcat gcatccattt tttgagggta gaaagcaatg
     5941 tggtctttga tcgattcata tccaggggta gggtctggat aagcccatgc agcgttagca
     6001 gcttttttgc cgccaactat tatagtgtaa taggaagcaa cacctttcca ctcacacaca
     6061 gtcctcttcc tagttattgt gaggtactca gtcttaattt ctttgggagg taagtaataa
     6121 acaggggcac cgcttgtttc taataccctt ttcgcattgc gcgtgtctgc gataactaca
     6181 ttgttgaaaa tcaccttgat atgctttgtc gtatcctcaa cgcggggagg ccgtgggtaa
     6241 tcccaaacag attcctgccc tggttctggc tcaatacgtt ttggttttat gatcatttcg
     6301 tgattcgtgg atcgtggttt ttgtatcgtg tttttgtgaa tcttgtttct atcctcgaat
     6361 cacgaaccac gaaccacaag ctttactgtt agcgaacaga aagatactgg atgctgaact
     6421 tgcactcgtc atcagtccaa acatgcttga ctttaaagac gtcagaaacc agttgctcaa
     6481 atttttcaag tgtgtactta taagaatatt cggtcaaaat actttcgccc tttttgaaga
     6541 agaactcgat gttatcaaca tgcacaaact gatttcttaa actaatgagg tgcatttcga
     6601 ttctttcttc atcactgttg gagaaagcat agtgactcca ttgatctagt tcaaaatctg
     6661 tgccaagttc aagattgaga cgcctaagta tgttcaggtt gaattcagca gtaatcccgt
     6721 tcttatcatt gtaggcagcc tctaaaacac ttttttcctt tttaagatct acaccgatca
     6781 aaagcccgcc tgtcttatct gctattcttg caattcgggt aaggaaagta gttgcttctt
     6841 taggggtaaa atttccgatt gatgagcctg gataatatat aaccttatgt gaccatggga
     6901 ggtttaagta gggtaaatca aatcgctgag tatagtctgc gtatacaggc acaatcttaa
     6961 gttgaggata atcttttgca agtgtagtta cggatttcat taagtgctcg gccgaaatat
     7021 caactggtac atatccagcg ggttcttcaa gatggtcaag taataatcgt atctttttgc
     7081 tgctaccact gccgagttct attaaaagac attttttgct caggcaagcc gagatttctt
     7141 caatatattt attcatgatc tccatctcgg tgcgcgttag atagtattct tctagttcgc
     7201 aaatctggtc aaagagccca gagccccttt cgtcataaaa gagcttacag gggaggtgct
     7261 tctgcggttt ttggagacct tcgagcacct cggctagaag aatttcctct tcaggttcat
     7321 atttttgtag tgtccttagg ttgttattca tctttagcca gccttattcc catgaattgc
     7381 caccgtgcat gaggtggaaa gaaattacga tatgtttttc ttatgtgaga catcgatgtg
     7441 gcgcaggagc ctcctctaag aaccatttga ttactcatga actttccgtt gtattctccc
     7501 aatgcacccg gtagggtttt aaatcccgga tatgggatgt atggactctg tgtccactcc
     7561 caaacatctc caaacatctg taaaagcccc ctatttgtag aatcttgatt tagtgtacga
     7621 gggtgaaagt ttccactttc tacaaaattc ccttctatag gtaatttaga tgatgcgatt
     7681 tcccattcag attcagaagg gagttttacg cctttccatc gcgcatatgc atctgcctcg
     7741 taatagctca cgtgggtgac gggttcgtct agatcaactt cacgcatacc tgaaagtgta
     7801 aaataccacc aatgccgatc tcccttttcc caataaagtg gagctttcca atcattcgcc
     7861 tcaacagtat accaaccatc agacagccaa agttctggtc tttcgtaact gccatcctca
     7921 atgaatgcaa gatactcacg attggtcacc aagcggtatg ctaatttaaa actatttaga
     7981 tacaccttat gacgagggga ttcgttatcg aaggcaaaac catttccgtc gtgcccaatc
     8041 caatatgccg cttcaggaaa tcggacccat ctcatttcgg gtattgtaaa aggcctaggg
     8101 ctatcatgtt tgatatgaag atatacagga cgaagggggt taactgagaa cacatgttta
     8161 atatctgtaa gtattaattc ttgatgctgc tgctcatgat tgagtccgat ttcgattaca
     8221 ggtgcaatct cctccatatg cgcctgattc gccttttcaa gaaattccaa catgtgctgg
     8281 tctacatgat gtctatagga atacacctct tctacagttg gtcttgataa aagcccacgt
     8341 tctgggcgaa agtgtctttt tccaacctga acataataag agttaaaaag gtaattatat
     8401 tgtggatgta gtgacttata atgtttattt actttattaa gtatgaatgt ctcaaagaac
     8461 caacttgtat gtgcaaggtg ccatttcgtc gggctcacat caggcattga ttgaatgacg
     8521 taatcctctg tggcaagtgg tttgcaaagg gtttcagtca actcccgaac gatttgatat
     8581 cgtttgacaa gagaactatt gatagattca ctttgatcct tttctgatac ttttgttatg
     8641 gtttttttcg tattcatttt cttctttctt attttatatc aaattttcta tgtaaaaaca
     8701 agtgcaccta tgtgcagatt actttaattt ctaacttctt taggtaatat ttattggagt
     8761 ttaaaaaata tatagctgct atgataaatc tatccttatg aaaaataata aaatccatgt
     8821 cgttcttttc attataactc tgggtacaac ttttttagcc ggttttcttc aaggtgggaa
     8881 tgtcacaagc ggaattgcat tttcagcagc cctcatattt attctaggcg cgcatgaaat
     8941 ggggcactac gtttacggga aaaggtatgg tgttgatatt acgcctcctt atttcatccc
     9001 agccccgccg gttatttctc ctataggtac atttggtgct tttataaaaa ttaagtctcc
     9061 aatcgctact aaaaaagccc ttttcgacat aggaatagca ggtcccctgg taggtatttg
     9121 tgccgcaatt cctgtcatta taataggaat caagctatcg agtattgttg atccttcgag
     9181 tcgtgaattt gagggtggaa tgactctcgg ttcaccttta attttctcgt ttcttgcaga
     9241 tacctttttt gggaaggttc cggatggtaa agaccttctt ctacatcccg ttgcattcgc
     9301 tggatggatc ggactctttg tcactgcgct taacttgatt cctgcaggac agctagacgg
     9361 cggtcacatt atgtatgcac tttttccaag aaagtggcat cgtcgtgctt ctgtttctat
     9421 gatttttttt cttttaattc ttggcatagg cactcagccg attattcatc tattacaatc
     9481 tctatggaaa ggaattcctc tcgaaacata taaggatcta cttacctatg atgggtggct
     9541 tggatgggtt atgtgggcga tgcttctttc aattatgggg acgagacatc cacctactat
     9601 ggatgataat atttctcttg atggtaagag aaagttttta ggcatagttg cgcttcttgt
     9661 ctttatcggg tgctttacac ctgtgcctat aggtttaccg aaatcgtaga aaagaagact
     9721 cgttgtaaca gaagtctagg gtttctcaaa tcagggttaa cttatgagtg ataaggttgg
     9781 attaataacg ggcggtgctc gtggtattgg acgtgcaatt gcccttgcct tggcttacca
     9841 aggttggtct attgctttat gctatcgaac cagtgaaaaa gaggctgtaa aaattgtgaa
     9901 tgaaatagag caaaaaggat cacgtggcct tcgagtgcgg tgcgatgtat ctgatcctga
     9961 agcggctcaa gcctttgtaa agcatgtcga gaatgaatgg ggacatatag atgctttaat
    10021 taactgcgca gggccatacc accgtgtcaa tctccttgag gagacaatcg aagggtggca
    10081 ctcaatgttt gataataatc tccacccagt tttttaccta agccgagctg ttgttccagg
    10141 tatgaaggag cgtaaatggg gccggattat aaatttcagt atggcaaatg cagaccagct
    10201 tgttgctcaa cctgaaatta cagcacacta tatagcaaag gctggaatac taatcttaac
    10261 gcgttcactt gcgcgtgttc ttgctccata tgggataaca gtcaattcta tatcccctgg
    10321 ttttatcagt tcaggaagcg cgccggatga tgagcttgaa aagatggcaa agaggattcc
    10381 cgcaggttat gtgggaggta ctcaagatgc tgtaacagtc gcaacctttc ttctctcaga
    10441 ggaggcagga tacattaatg gagctaatat tcaccttagt ggtgcttggg gtatatagta
    10501 agatagttaa aaacatttaa aaagagtatt tgaaatattt ctagatttgg gtataggtat
    10561 ttctctgagt atttctcatt agcttacgat agggataaat gatgacccgt cattttaagt
    10621 agagtgttga gagaagaatc agggtattgg tgctgggaag gcgtttgcct ttctcccctt
    10681 ggatacttgc cgtgacgaat tttagtgtag cgactgttag aggtatattt gtaaaaaagg
    10741 cttagcttgt actaaatacg gatactagtt tattatttgg tttgagtgtt ttaatcttaa
    10801 ccgaaaaccc taagaggaga tataagtgtg gctaaagaaa ctttgacgat tactgacaat
    10861 aggacaggga aaacctacga gattcctatt aactctggga ctattagagc gatggatttt
    10921 cgccaaatta agatctcaga agatgatttt ggaattatga gctatgatcc agcgttgggt
    10981 aacaccgcat cctgcaatag tagaataact ttcattgacg gagagaaggg agttcttcta
    11041 tatcgagggt atcccattga gcaactagcg gataaaagca attatcttga agtagcttac
    11101 ctactcttat atggggaact accttctaaa tctcagtatg atgcatgggt ttacaatatt
    11161 acgcatcata cgatttttca tgagaatata aaaaaattta tcgacggttt tcgttacgat
    11221 gctcatccga tgggtatgct cctaagcgct gtcggagcat tatccacctt ttatcctgaa
    11281 gcaaagaata tctttgaccc ggattcgaga aagaagcaat tccatcgttt gattgcaaaa
    11341 atgccaacgt tagccgcctt ttcttatcgc caaacccttg gcttaccata cgtgtatccg
    11401 gataacgatc tcagctacac cggaaatttt ttgaatatga tgtttaagat gactgaactc
    11461 aaatataaac caaatccagt tattgaaata gcgatagata tactatttat acttcatgct
    11521 gaccatgaac agaattgtag tacaaacgcg atgcgcagcg ttggaagttc acttccagat
    11581 ccttattcag ctaccgctgc ggcaattgct gcactttatg ggcctcttca
//
LOCUS       DATDRL010000344         4966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_277219, whole genome shotgun sequence.
ACCESSION   DATDRL010000344 DATDRL010000000
VERSION     DATDRL010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277219"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..535)
                     /locus_tag="VI935_11520"
     CDS             complement(83..535)
                     /locus_tag="VI935_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HLE26256.1"
                     /translation="MKGWFIKFFASFFYLGYSPIAPGTAGTIGGVILFYFLSNFSLYI
                     YISFFLGFIIFSVWVSTKASGIFGESDPKQVVVDEVCGYLVTMFLITPSLKNMILGFF
                     LFRLLDIVKPPPVRSSERLPGGFGIVTDDVVAGIYANIIMQVITRVIG"
     gene            complement(702..1079)
                     /locus_tag="VI935_11525"
     CDS             complement(702..1079)
                     /locus_tag="VI935_11525"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HLE26257.1"
                     /translation="MNSLNGVKLTKGGEAERSAREVRLYLEGKLKKFHANLDLSSGTP
                     FQTSVWRELLQIPYGKVETYREIAKRIGNPSAARAVGNAVGANPIPIIVPCHRVVATN
                     GLGGYSSGIEIKKKLLQIERVSK"
     gene            complement(2121..2675)
                     /locus_tag="VI935_11530"
     CDS             complement(2121..2675)
                     /locus_tag="VI935_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26258.1"
                     /translation="MRIVLFIVTLMVFFAFRSEAQILGGVGVGASLEVVPISHRTISF
                     RGGVFSAKFLCGEIPHDQANPQNPIAGELLVPGTYLTAINIYNPNNFTVRFAKKAIIT
                     NPQRERRGAVSKQIIETLGPMQGLEVDCCDIAQLFTTGLECNAENLFGGFSKGFVDIS
                     INPFVPLDVVGVYTLKNVFIGGPS"
     gene            3505..4641
                     /locus_tag="VI935_11535"
     CDS             3505..4641
                     /locus_tag="VI935_11535"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HLE26259.1"
                     /translation="MPSKLSEEIAPFFVMDVLERAKEIEAQGKKVIHFEIGEPDLPTP
                     KIICDEAIEAIRVGDTKYTPSLGIPELREAIAEDYKEKYGVNIPPGRVVITSGSSPAL
                     FLSMLSLLEHGDEVIITDPHYSCYPQIIKVAGGLPKPVRIYEEDGFQIDIDSLKKTTS
                     NKTKAIVINSPSNPTGVVLEPNVIKEISELGLYIISDEIYHGLVYEGKAHTIYEFTDR
                     AFSVNGFSKLYSMTGWRLGYLIAPEDFIRPTQKLQQNLFISPNPFVQRAGVAAIRKAK
                     REAKEMVQIFSERRKKMIEGLRGLGFVIRSEPKGAFYVFVNAKSLNDRSQELAFDILD
                     KSHVAVTPGIDFGYGGEGYLRFSYTTSLDDIGEGIKRLGEYIHK"
     gene            4723..>4966
                     /gene="pgk"
                     /locus_tag="VI935_11540"
     CDS             4723..>4966
                     /gene="pgk"
                     /locus_tag="VI935_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HLE26260.1"
                     /translation="MMNLVSIDEIDIKEKRVLIRVDFNTPLNKDGEVANDTRILAALP
                     TIEYALREKARVIIASHLGRPGGKINTKLTLDPVGRK"
BASE COUNT         1530 a         1050 c          995 g         1391 t
ORIGIN      
        1 ctcattatct catcgcctgt tgttatgatt tcgatcttca tctaactgag tagagacgac
       61 ccggcgggtc gtctctacat tattatccaa tcacccttgt aataacctgc attataatat
      121 ttgcgtaaat cccagctaca acgtcatctg tcactattcc aaacccacct ggtaagcgtt
      181 ctgagcttct tactggggga ggttttacaa tatctaataa tctaaagaga aaaaaaccca
      241 aaatcatatt tttcaagctt ggcgtaatga gaaacattgt tactaaatat ccgcaaacct
      301 catcaaccac cacctgtttt ggatcgcttt ccccgaatat acctgatgct ttagttgaaa
      361 cccatacaga aaagattata aacccaagaa aaaacgaaat atagatgtat agagaaaagt
      421 tagataaaaa atagaaaagt attactccac caattgtccc tgctgttccc ggtgcaattg
      481 gcgagtatcc cagataaaaa aagctcgcga aaaacttaat gaaccatcct ttcatatcaa
      541 atccgactta acagcgactg tgtaatggag cgaagaatcc ctgggtttta tgttaagatg
      601 cttcacggag tttatccctt cggcaaactg agggcaggct ctgagtgtag cgaagggttc
      661 atcacgacca atagatagta attaaccggg tttgataaag cctacttaga cacacgctca
      721 atctgaagaa gcttcttttt gatctcaatc cctgaagaat atccacccaa gccatttgtt
      781 gcaaccactc tatgacatgg gacaattatg ggaattggat ttgcgcctac tgcattgcct
      841 acggctctag cggcactagg atttccgatt ctctttgcaa tttctcgata ggtttccact
      901 ttgccataag gaatctgtaa aagctccctc cagacagagg tttgaaatgg agttccagaa
      961 gataaatcca ggtttgcatg aaatttcttt aacttgcctt caagataaag gcgaacctct
     1021 ctagccgacc tttccgcttc gcctccttta gtaagcttaa ctccgttgag gctattcata
     1081 aaccgattaa aattatcgct cccaaaaata atccttgaaa cgcctttagg agtgctgata
     1141 acgaagatgg atcctaaaac agaattaaat tgaaaaaaat tagacaggtt cattgaacta
     1201 ttcgctaata attttcataa cagcattcgt aatgtcaagg aattggtgga aggttgtctt
     1261 tgcaaagtga taatgtcact attttaatgg cttaactttg gagtaataaa gccctctgca
     1321 gttgtttttt tatggatata atgtagcggc gaccctcggg tggccataaa gaaaaatgtg
     1381 gaggtctaaa gacctcggct aggttttgta cgagcggctt agccacgact aggtcgcttt
     1441 cattctgtat attttataat gttataccac ttccatttga taactgttca aatattgtag
     1501 gcacagactt gtctgtgcta tttttgggca cgaacaagtt cgtgcctaca gtgctcattg
     1561 gtcatttcct aatttgaaat ggtaatataa tgttaataat cactctgcat tttgctctat
     1621 attataccga ttagattggt ctactgtgaa taatgcgagc tgtcattgcg aacacattcg
     1681 ctgagcctgc agcggatagg agggacattt ccttcgttaa actcaggaca ggcttgtccc
     1741 gcaacaggat gcggggtttg aaaaccccgc ctatcgatat actccgtgaa gcaatgacag
     1801 ttgtgacgct ttaatattaa gaccataccc tgcagcttgc agggttattg aatctgggct
     1861 gagtaccttt tgttgcaagt gtatagaagt aaaatttggt aaacacgcct gatgtagggg
     1921 tttgattaat caaaccccta cttgtgtact aaacttggtt aagctgcaac taattcccag
     1981 acccgatttt ttattttcgt cccgacacgt tccttattac cgctcaagta cagtttaggg
     2041 tcttctacac attcaggatc acaacaatct acttaggtaa tccagaaaga acgttcttta
     2101 atgtgtaaac ccccaccaca tcaactaggt cctccaataa aaacgttctt taaggtgtaa
     2161 acccccacca catcaagcgg aacgaagggg ttaattgaaa tatcaacaaa acccttggaa
     2221 aatcctccga ataaattctc tgcattgcac tcaagaccag tggtaaagag ttgcgcaata
     2281 tcacagcagt caacctcaag cccttgcata gggcctagag tttcaataat ctgcttactg
     2341 accgctcctc tcctctcacg ctgagggttc gtaattatcg ctttcttggc gaatctcaca
     2401 gtaaaattgt tcgggttgta aatgtttata gcagtaaggt aggttcccgg tacaagaagc
     2461 tcgcctgcaa taggattctg ggggtttgct tggtcatgcg gtatctcccc acagaggaac
     2521 ttagccgaaa acactccacc cctaaaactg atggtcctat gactaatcgg caccacttcg
     2581 aggctcgctc ccactcctac cccaccaaga atttgcgctt cactcctaaa ggcgaaaaac
     2641 accatcaatg taacgatgaa aagtactatt ctcatcttat cttacctcct tttatttaat
     2701 gcttgcttct cttatttact cctttagatt gggcaaatct cgcttcacgt catcattctg
     2761 aaactaacaa tcaagtgaca tcacctcctt tcaacctcaa acccaaactg cctttttaaa
     2821 aatccttaat aaacagagca taaaaaactc tcttaggaat gcagaatatg tttggtagga
     2881 agagcaggta ggtagaaaaa ccactcctgg ctaaatttgg aagcagtaaa aaatgactta
     2941 aaatcccttc tatagcatga aatgatcttg atataactgg tgaaaggtaa tatttgggta
     3001 tattgaaacc taccacattc ccatacctca tcctctgact gtatctttat taggtagcac
     3061 acttaggggt ggatgtcaac tcccgatctt ttgcctattt tatatgatgc agcaagacaa
     3121 ttcataactt tgatcattgt gaaaaccgag accacatcac ccataaagca agggactgag
     3181 tttagaagaa atgcaaaact gagtatggtt ttgagccgaa gccctaaatg cattaaagac
     3241 ccaagctacg gatcccatta taaatcacga gatgactcgc tcaccttgct tcttcaggac
     3301 gacacactta tgcagtcatg gtgaggagcc cttcatctag ctcaggataa actccgcgac
     3361 gaagcattct gaccacgagg tgtcatctcg aactcaagtg agtgatctag attcctcact
     3421 aagttcggaa tgacaattac acaatgctgt gtggctaact ataatttata gacagcgatg
     3481 gcctttttgc ttagaataaa tatcatgccg tcaaagctct cggaagaaat tgccccgttc
     3541 tttgttatgg atgttttaga gagggctaag gaaatcgaag ctcaaggaaa gaaggttatt
     3601 cactttgaaa ttggagaacc agacctcccg actccgaaga tcatctgtga cgaagctata
     3661 gaagccatta gagttggaga cacaaaatac acgccgagtc tcggaattcc agagctaaga
     3721 gaagctatag cggaagatta caaggaaaaa tacggggtta atatacctcc tggtagagtt
     3781 gttattacct ctggtagctc ccctgccctg ttcctctcta tgctttcact tctcgaacac
     3841 ggagatgagg ttataattac ggacccacat tattcttgct atccacaaat tataaaggtt
     3901 gcgggaggac tcccgaagcc tgtgagaatt tatgaagagg atggttttca gatcgatatt
     3961 gatagcctaa aaaaaacgac ctccaacaaa acaaaagcga tagttataaa ctccccatca
     4021 aatcctactg gagtggttct agaaccgaat gtgataaagg aaatatcaga actcgggctt
     4081 tacataatct ctgatgaaat ctatcacggg cttgtttatg agggaaaggc gcacactata
     4141 tatgaattta ctgatagagc gttttcagtt aatgggtttt ccaagcttta ttcaatgaca
     4201 ggatggcggc ttgggtattt gattgccccc gaggatttca ttcgccctac tcagaagctt
     4261 cagcaaaacc tttttatctc accaaatcca ttcgttcaga gggcgggggt tgcagccata
     4321 aggaaagcta agcgggaagc aaaagagatg gttcaaatct tcagcgaaag aagaaagaag
     4381 atgattgaag ggcttagagg tttggggttt gtgattcgtt ctgaacctaa aggtgcgttt
     4441 tacgtatttg ttaatgcaaa aagtttaaat gatagatcac aggaattggc atttgatatt
     4501 cttgacaaat ctcatgtagc ggtaactcct ggaattgatt ttggatacgg aggagaggga
     4561 taccttaggt tttcttacac aacatctctt gatgatatag gagagggaat taaaaggctt
     4621 ggagaatata ttcacaaata aagaagcctt cagctattag cgatcagccg ccagtaaaag
     4681 aagaaagctg atagctaact gctgactgat gatagcaaaa aaatgatgaa cttagtttcc
     4741 attgacgaaa tagatataaa ggaaaaaagg gttcttatca gggtggattt caacacacca
     4801 ctcaataaag acggtgaggt cgcgaatgat acgagaatcc ttgcagcact acctactata
     4861 gaatatgcgc ttagggaaaa agcaagggta attattgcct ctcacctagg aagacctggc
     4921 ggaaaaataa acacaaagct tacgcttgat ccggtgggaa gaaaac
//
LOCUS       DATDRL010000345         3509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1191925, whole genome shotgun sequence.
ACCESSION   DATDRL010000345 DATDRL010000000
VERSION     DATDRL010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191925"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /locus_tag="VI935_11545"
     CDS             <1..1503
                     /locus_tag="VI935_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26261.1"
                     /translation="SGNIYAGTASGVQVQVPAAPATQVVAQSGVVTPFSKEERAWDLN
                     LPEELPMAQPATMMQQRPPSRETGVPPKTTGIPMAPNYVYKITKEGLAQKIFEVDQAF
                     ILGISLDTQDNLYVVTGNKPGVYKICKDETSSSVVDVEEVQVLCCLNTGNNELYVGTG
                     NVGKVYKILPAFVKNGDFISTVLDTTTVSDWGCIYWQAAQPEGTKVTLATRSGNCENP
                     DYTWNRWSIPYQSPGEKITSAPARFIQYKANLQTINTDTTPAINMVSLSYLPKNQPPK
                     IVNFTIEKEPSPASQKLTEVKADGKTEAKPQTPAIQKPHHQVAQKNVQWDVEDPNNDS
                     LQLTIYFKGDNEKAWKVMDKNTQKKGAYPWDTLRLPDGKYQIKLVASDEPDNPPETAL
                     SAENTIQPIVIDNARPIIKSMNAAMGTDGRYVISGAIKDEYSNIIKVQYTIDGQDWTS
                     AYPVDGVFDSQEESFQITTKHLTPGDYTLIVNAFDSEGNIGVEKIMIEVK"
     gene            1604..2278
                     /locus_tag="VI935_11550"
     CDS             1604..2278
                     /locus_tag="VI935_11550"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLE26262.1"
                     /translation="MNKKAILFLELKMFSKLVKIQVKERSIAMKDIKTEKGKKINRKS
                     LIVALDVAQEKHVVYIRCPDGRGEKPFVVFNRREEYEQMWERISQTKEAYGLEEVVVG
                     FESTGPYAEPLVHYLMKKEVNLVQVNPMHTKRVKEIRGNSPNKTDEKDPEVIADIIEL
                     GNYLTVVIPEGVSAELRRLTQSRERAVGRRTMPVNQVHGLVSVIFPEFLMVMKDIKTK
                     TAHTIF"
     gene            2362..2934
                     /locus_tag="VI935_11555"
     CDS             2362..2934
                     /locus_tag="VI935_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLE26263.1"
                     /translation="MGKERAEALYEAAVNTVGVKEGQGSIVFEIKQLMTGIEETERFI
                     DEMEERMKEHLKEIPYSRCMLSIQGVGEVTVGGLIGEVGDFTKYRTIAEITKLAGLDL
                     FEVSSGKHKGERHISKRGRPLMRKLLFYAAINVVREGRIMHERYERYLQRGMPRIKAL
                     IAIARKLLGILFALARDQRNYEEIPLKEAA"
     gene            3369..3446
                     /locus_tag="VI935_11560"
     tRNA            3369..3446
                     /locus_tag="VI935_11560"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3404..3406,aa:Pro,seq:cgg)
BASE COUNT         1138 a          632 c          938 g          801 t
ORIGIN      
        1 tcaggcaata tttatgcggg aacggcgtct ggtgtgcagg tacaggtacc cgcggctccg
       61 gctacccagg tcgttgccca gtccggagtt gttacgcctt tttctaaaga agaaagggcc
      121 tgggatttga acctccccga agaattacca atggcccaac ctgcaactat gatgcagcaa
      181 aggcctccat cgagagaaac tggggtaccg ccgaaaacta caggcattcc aatggcgcca
      241 aattatgttt ataaaatcac aaaagagggg cttgcgcaga agatattcga ggtcgatcag
      301 gcctttatcc tcggtatatc tctggatacg caggataacc tgtatgtggt tactggaaac
      361 aaaccaggtg tgtataaaat ttgcaaagat gagacttcaa gcagcgtggt ggatgttgaa
      421 gaggtacagg tgctttgctg cttaaatacc ggcaataatg aattatatgt gggtacaggc
      481 aatgttggaa aagtttataa aatattgcca gcttttgtaa aaaacggcga ttttatttct
      541 accgtgcttg atacaacaac tgtttcggat tggggatgta tttattggca ggcggcacaa
      601 ccggaaggca cgaaggtaac attggctacc cgttcgggaa attgtgaaaa tcccgattac
      661 acctggaatc gttggtcaat tccttaccag tcgcctggag aaaaaataac aagcgcccct
      721 gcgcgtttta tacaatataa agctaactta caaacaataa ataccgatac gacacctgca
      781 ataaacatgg tgtcattatc gtatttaccg aaaaatcagc cacctaaaat agttaatttt
      841 accatagaaa aagaaccttc ccctgcatca caaaaactta cggaggtaaa ggcagacggc
      901 aagacagaag caaaacctca gacgcccgca atccaaaaac cgcatcacca ggtagctcaa
      961 aaaaatgtcc agtgggatgt ggaagatcca aacaacgact cccttcagtt gacaatatat
     1021 tttaagggtg acaatgaaaa ggcatggaag gttatggata agaacacgca aaaaaaaggc
     1081 gcttatccat gggataccct gcggctgcct gacgggaaat accagattaa gctagtagcc
     1141 agtgatgaac ctgataatcc accagagact gctctcagcg ctgaaaacac tatacaaccc
     1201 attgtgattg acaatgcacg gccgatcata aagtctatga atgctgcgat gggcacggat
     1261 ggaaggtatg tcatttcagg tgcgataaag gatgaataca gcaatattat aaaggttcaa
     1321 tacacaattg acgggcagga ttggacatct gcctatcccg tagacggggt cttcgacagc
     1381 caggaagaat cctttcaaat aaccaccaaa cacctcacgc cgggcgatta cacccttatt
     1441 gtcaacgcat ttgatagtga agggaatatt ggtgtagaaa agataatgat agaggtgaaa
     1501 taaaaaataa tagttcactg cataaggatg cggagaacgc gaagttacaa cccactaaat
     1561 cccccttagc aaagcttgct ataatgtaga aagtagttga gctttgaata aaaaagctat
     1621 actctttctt gaattgaaaa tgtttagtaa gttagtaaaa attcaagtca aagaaaggag
     1681 tatagccatg aaagatatta agacagagaa ggggaagaaa atcaatagaa aaagcctgat
     1741 agtagcattg gatgtggcac aagagaagca tgtagtatat attcgatgtc ctgatgggag
     1801 gggagaaaaa ccatttgtgg ttttcaatag acgagaagag tatgaacaga tgtgggagcg
     1861 aataagccag acaaaggagg catatggact ggaggaagta gtggtaggat ttgaatctac
     1921 ggggccgtat gcagagccac tggtgcatta tttgatgaag aaggaagtaa atctggtgca
     1981 ggtaaatccg atgcatacaa agagagtaaa ggaaataagg ggcaattcac ctaacaagac
     2041 ggatgagaag gatcctgagg tgatagcgga cataatagag cttgggaatt atttgaccgt
     2101 agtgataccg gaaggggtat cagcagaact gaggaggctg acccagtcgc gagagagggc
     2161 agtagggaga cggacaatgc cagtgaatca agtgcatgga ttggtgagtg tgatatttcc
     2221 tgagtttttg atggtgatga aggatatcaa gacgaaaaca gcacacacta tcttttgaaa
     2281 cactgtccaa gaccacagga catagtggag cttggatatg agagattgga agagacgttg
     2341 aggaagataa gtcgggggag aatgggtaag gagcgtgcag aggcgctgta tgaagcagca
     2401 gtaaacaccg tgggagtcaa agagggtcaa gggagcatcg tgtttgagat aaaacagttg
     2461 atgacgggaa tagaagaaac agagcggttc atagatgaga tggaagaaag aatgaaggaa
     2521 catctcaaag aaattcctta cagtcgatgt atgctatcaa tacaaggggt aggagaggtg
     2581 acggtaggtg ggctgatagg ggaagtggga gattttacga agtaccggac gattgcggag
     2641 attacgaagc tggcggggtt ggacttattt gaagtgagtt cagggaagca caagggagag
     2701 cgacatatat cgaagcgggg acgtccgttg atgaggaagc tgttgtttta tgcggcaata
     2761 aatgtggtga gggagggaag gatcatgcat gaacgatatg agagatacct ccagagaggg
     2821 atgccgcgga taaaggcgct gattgcaata gcacgaaaac tcttagggat tttatttgcc
     2881 ctggcacgtg atcagaggaa ctacgaagag ataccattga aagaagctgc gtaagctgtg
     2941 tattcgtgga ggcggtaagg attatcgagt cccccataaa ggcgaccgag tcgaccttcg
     3001 aacaaagcat agttaacttt accgcctgca ccatgagtcc caatgaagca aggttagggc
     3061 tcgcaagaga ccctcaaagt actagggttg gacttggtgt tccacaaaaa cagggaaggc
     3121 aaatactttt acggagctaa agcgagcggt gattgtggct aactccagtc gggctacgcc
     3181 ctccttccgt tagccacaat aaaattatat ttattataat tgttataaaa gatacttgac
     3241 tagaataaac tagggggact aagggggttg ttatgcgatc ttagcgtgct ctgcggtgaa
     3301 ctgaactgtt acgaattttc tttgcgttct tcgtgtcttt gtggtgaaat attattataa
     3361 aaatggggcg gggcgtggct cagtttggct agagcgcgac gttcgggacg tcgaggtcgg
     3421 aggttcaaat cctctcgccc cgaccaaaat taaaaagaat aaaaatattg aaggaaaggt
     3481 tgaggttaga ggcgcgcttc ccgatctca
//
LOCUS       DATDRL010000346         5591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3571957, whole genome shotgun sequence.
ACCESSION   DATDRL010000346 DATDRL010000000
VERSION     DATDRL010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3571957"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /locus_tag="VI935_11565"
     CDS             <1..133
                     /locus_tag="VI935_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HLE26264.1"
                     /translation="IDVKRKGKVRRAKLYYLRGLSRKASRIKERTIFDKEIKDNSQS"
     gene            complement(249..953)
                     /locus_tag="VI935_11570"
     CDS             complement(249..953)
                     /locus_tag="VI935_11570"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HLE26265.1"
                     /translation="MSTPEEVVDRAKGGKSPSASKLLGRDIKTWWILIMRPIEDYLID
                     KNVHPNVLTVTSLIVSAIAGLLFYKGWIFFAGIILLAGSNFDMLDGRIARAQGLSSEH
                     GAFFDSCLDRFAEIFIYLGLIGYFHISVFSYVVFLILGATTMVSYTRARAEGLGVDCE
                     VGMMQRTERMVYLGAFSVFNLFGNLVTATLGFKPEDYLLKFVLALILIFSSYTAIERM
                     IHVMKRLKEREGKKRE"
     gene            complement(1156..2040)
                     /locus_tag="VI935_11575"
     CDS             complement(1156..2040)
                     /locus_tag="VI935_11575"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HLE26266.1"
                     /translation="MADTENNPSNRQIVYLFLGFVILFIFTFSLGVIVGKGLGGSKTQ
                     ITSEAVPPKTSGKDVQEPEETTKIITGRELQSESPTPPIREEEMIEVREEIIIPTPLS
                     EPAEEQIETKRDKPTSSELLSKQPPSTVEPIEKKPELETKKVEPIKKDIAASPTILPT
                     PVAPAVKKPELQKKEIEAKITPTPLPIEKQTKLETKEAEPIKKNIAALPPIQSGGRYT
                     VQIAAFQKEEEAKQIVNNLKSKGYPAFIKTAEVPGKGTWYRVRIGTFTTRVTAKLYAN
                     NLKNLEPSIKFVFITVND"
     gene            complement(2044..3729)
                     /gene="argS"
                     /locus_tag="VI935_11580"
     CDS             complement(2044..3729)
                     /gene="argS"
                     /locus_tag="VI935_11580"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HLE26267.1"
                     /translation="MKEEVQKIVVESVDILRRRNDFESTSREIEITIPKRREFGDFST
                     NIAIILASELNKNPHEVAELIIDNISLEGRDLFKKIEIAGPGFINFFINERAIISRLM
                     VIERLGENYGKSNLGKGEKVLVEFVSANPTGYLHMGHARNAAVGDAISNILDGVGFNV
                     TREFYINDIGRQMELLGVSIYRRYQELFGIKKEFPGDGYWGEYIKDIANEIRGKRGRE
                     LIVEGALEQESIDYCKEYAKDRLLQEIKKDLNDFGVNFDNWYSEQNELHRALPEFRGG
                     NRIGSIKNELMFKEALEERDGALWFKATEFGDSQDWVLVRRDGASTYFFSDIAYHYDK
                     IQRGFKRLIDVWGADHHGHFSRLLAALRALRLDDFSLKVVLIQFVRLIKDGQEISMSK
                     RAGSYLTMREVMEEVGRDVMRFFLLMRSSESHLDFDLDLAKKESSENPVYYIQYAYAR
                     IGSVLEKAKERELSPSKESLNLLSQPEEIDIIKKLLTFPELVRESALSLAPHKLVFYL
                     QEIAADFHVYYNKIRIISEDQELSLARLYFVNCIRTVIRNGLRLLGITTPERM"
     gene            complement(3810..4433)
                     /locus_tag="VI935_11585"
     CDS             complement(3810..4433)
                     /locus_tag="VI935_11585"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLE26268.1"
                     /translation="MNLILIRHGETEWNRVGRCQGFSDVELNDNGREQIMELAKSLMN
                     ESISAVYSSDLKRALDTAIAIAHHHKLAVEVDPDLRELNQGDFEGLTFVEIRERYSPL
                     LNKWAESPEVVKLPGGESLKELQERAWRAIERIYIKHQNQTVVAVSHNLTIITLLCRF
                     KGIGLEEFRSFKLRAASKNIILFRNEHFNVNVLNDVSHLTQELLTEQ"
     gene            complement(4476..5429)
                     /locus_tag="VI935_11590"
     CDS             complement(4476..5429)
                     /locus_tag="VI935_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HLE26269.1"
                     /translation="MSASKDGISGVSWFWGVVLSLSLHIVIFLMAIFWGFGSPMYSGE
                     PESIEGTLVSISELGQPGGEVATEKEEVKPQVPETKEEPAKVEEKKPEEKIQVSKPEK
                     KEPQEEPKKTELKKEETPKEKPKQDDTIVLQEKEKKKEKKEIAKKPEPTEPPKTKPQK
                     KVESKKETFEDVKNRVIGEMQKQKLLKEFQQKRVASADSSAERKGVQKGAGYSTGGSR
                     GGSSGVVAELFVRRVREEIKNNWAIPENIPIDGSLKAVIVFKLDERGRPSDVKVDIPS
                     GNTAFDDFCVKAIYRASPLTPPPPELLEEARTEGLEVEFTP"
     gene            complement(5426..>5591)
                     /locus_tag="VI935_11595"
     CDS             complement(5426..>5591)
                     /locus_tag="VI935_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HLE26270.1"
                     /translation="KRIEDGDVFLRADADVPYGDVMEVMAEIKKAGVKKLGLVTEPLS
                     SNSSQTRQKK"
BASE COUNT         1564 a         1283 c          886 g         1858 t
ORIGIN      
        1 gatagatgtt aaaagaaaag gaaaggtaag aagagcaaag ctctattacc tgagaggact
       61 ttcaagaaag gcaagcagaa taaaggaaag aacaatattc gataaggaaa ttaaagataa
      121 tagccaatct tagttagtat attaatttct tatttatttt ttcgactctg ccttagatta
      181 gccttggtat aatctgaaga ggaacccaac caaagcaggt tgatcttaaa ttgggtttag
      241 ccatatagtt actctctttt cttgccttct ctttccttaa gtcgcttcat aacatgaatc
      301 attcgctcga tagctgtgta agaagaaaat atcaatatca atgctaagac aaacttaagt
      361 aagtaatctt cgggtttaaa tccaagagta gccgttacca aattaccaaa aaggttaaaa
      421 acagaaaagg ctccgaggta taccattcgc tctgttctct gcatcatacc tacttcacaa
      481 tcaactccta aaccctccgc ccttgccctt gtgtagctta ccattgttgt tgcacccaga
      541 ataagaaaaa caacataaga gaatacgctt atgtgaaagt aaccaattag tcctaaatag
      601 ataaatatct ctgcaaatct atctaagcag gaatcaaaaa atgccccatg ctcactgctt
      661 aacccctgtg cccttgctat gcgtccatca agcatgtcaa agttcgagcc cgcaagtaga
      721 attattccag cgaaaaaaat ccacccctta tagaataata accctgcaat tgcagaaact
      781 atcaatgatg taactgtgag aacattagga tggacattct tatcaattag atagtcttca
      841 attggacgca taataagtat ccaccaggtt ttaatatcac gtccaagaag ctttgatgct
      901 gaggggcttt tacccccttt ggccctatct actacttcct ctggcgtaga cataacttag
      961 agactccatt aatcaggcga ttttctaaca atatgtttaa cctttacctt taaaaagaaa
     1021 tttaaatcgt tttatctctt aaacataaaa catttttatg agattttacc aaaaaagata
     1081 aatcagagca ttcatatgct gtaaagcctt tatatcaaat gtactattat ttatcactcc
     1141 acacaattaa aaaactcaat cattaaccgt gataaacacg aattttatag acggctcaag
     1201 attcttcaaa ttattcgcat agagtttagc cgtcactctt gtggtaaacg tccctattct
     1261 aaccctatac catgtgccct ttccaggaac ctctgccgtt tttataaatg ctgggtatcc
     1321 cttagacttc aggttgttaa caatctgttt tgcctcttcc tctttttgaa aagcagctat
     1381 ttgaactgta tatctccccc ccgactgaat agggggtaag gcagctatat tttttttgat
     1441 gggttccgcc tctttagttt ctaactttgt ttgcttttct ataggcaatg gggttggagt
     1501 tattttcgcc tcaatctctt ttttttgtaa ctcaggtttc tttactgccg gtgcaacagg
     1561 ggtgggtagt atagtaggtg atgcagctat atcttttttt ataggttcta cttttttagt
     1621 ttctaactcc ggtttctttt ctatcggttc aacagtagaa ggtggttgtt tggataataa
     1681 ctcactactt gtaggcttat ctctcttggt ttcaatctgc tcttccgcag gctcacttag
     1741 gggtgtcgga ataattattt cttcccgaac ctcaatcatt tcctcctccc gaataggagg
     1801 ggtgggactc tcactttgca attccctgcc tgtgattatc ttagttgttt cctcaggctc
     1861 ttgaacatct ttacccgagg tttttggagg cactgcttca ctcgttattt gtgttttaga
     1921 acctccgagc ccttttccca caatgacacc aaggctaaac gtaaaaataa ataaaataac
     1981 aaaaccaagg aaaagatata caatctgacg attgctagga ttattctcag tatctgccat
     2041 ttttcacatc ctctcaggtg tagtaatacc cagtagccta agcccatttc tgataaccgt
     2101 ccttatacaa ttaacaaaat aaaggcgggc taagctcagc tcctggtctt cgctgatgat
     2161 tctaattttg ttgtaataaa catgaaaatc tgccgcaatt tcctgcaaat aaaataccaa
     2221 cttgtgaggt gcaagagaaa gtgcgctctc cctcactaat tcaggaaaag ttaggagttt
     2281 ctttataatg tcaatctctt caggttgact taagagattc aaagactctt ttgaaggtga
     2341 taattctctt tcctttgcct tttccaacac gcttccaatt ctagcataag catactgaat
     2401 atagtacacg gggttttcgc ttgactcctt ctttgctaaa tctaggtcaa agtctagatg
     2461 gctctctgag cttcgcatga gaagaaaaaa gcgcatcaca tcacgcccta cctcttccat
     2521 aacctctcgc attgttaaat agctgcccgc gcgctttgac atagagattt cttgcccatc
     2581 ctttatgagc ctaacaaact ggataagaac aaccttaagc gagaagtcat cgagcctgag
     2641 tgctctaagc gccgctaaaa gacgcgaaaa gtgcccatga tgatctgctc cccacacatc
     2701 tattagtctt ttaaatcccc tctgaatttt atcataatga taagcaatgt cagagaaaaa
     2761 atatgtagaa gcaccgtccc tccttacaag cacccaatcc tgtgaatcac cgaactcagt
     2821 ggctttaaac caaagcgccc catccctttc ttccaaggct tccttaaaca tcaattcgtt
     2881 ttttattgaa cctatcctgt ttccacctct aaactctgga agtgctctgt gaagttcatt
     2941 ttgttcgcta taccaattgt caaaattcac accaaaatca tttaagtcct tttttatttc
     3001 ctgaagaagt ctatccttag catactcttt gcaataatct atggattctt gctctaaagc
     3061 cccttcaaca atgagctctc tacctctttt ccctcttatc tcattagcaa tatcctttat
     3121 atattcaccc caatatccgt cccctggaaa ttctttctta attccaaaaa gctcctggta
     3181 ccttctataa atagagacgc caagaagttc catctgtctt ccaatatcat taatatagaa
     3241 ctctcttgtg acattaaaac caacaccatc tagaatattg gaaatagcat ctccaacagc
     3301 agcatttctt gcatgaccca tatgaagata cccagttgga tttgcgctta caaactctac
     3361 aagcaccttc tctcccttac caagattaga ttttccatag ttctctccta gtctttctat
     3421 taccatgagt ctacttataa tagctctttc gtttataaag aaatttataa aacctggccc
     3481 tgcaatctct atctttttaa atagatccct tccctcaaga gatatattat ctattatgag
     3541 ctctgcgact tcatgtgggt ttttatttag ctcactagca agaataatag ctatattcgt
     3601 tgagaaatca ccaaattctc ttcttttagg aattgttatt tcaatctctc gagaagtaga
     3661 ttcaaaatcg tttcttcttc ttaaaatatc tactgactcg actactattt tttgaacttc
     3721 ttctttcatc taaactaaca aactacataa ttatttttgg actaacaata aacggttaaa
     3781 tgattataac ttgggattta atttaaactc tattgctctg taaggagctc ctgtgttaga
     3841 tgtgatacgt catttaggac attaacatta aagtgttcat ttctaaatag tatgatattt
     3901 ttcgaggcag ctctgagttt aaagctacga aattcctcaa gtcctatgcc tttgaatctg
     3961 caaagaaggg tgattattgt caggttatga ctcacggcaa caaccgtttg attctggtgt
     4021 tttatatata ttctctcgat agctctccaa gccctttctt gaagctcttt gagtgattct
     4081 cctcctggga gttttacaac ctcaggactc tctgcccact tgtttaaaag aggtgaatat
     4141 ctttctctta tctcaacaaa tgtaagccct tcaaaatcgc cctgattcag ctccctcaaa
     4201 tcaggatcaa cctctactgc aagtttatgg tgatgagcaa tcgcaattgc tgtgtcgagc
     4261 gcccttttta gatcgcttga ataaacggca gaaatagatt cattcatcaa agatttagct
     4321 aactccatta tctgttccct accattatcg tttaactcta catcagaaaa tccttgacat
     4381 ctgccaactc gattccattc agtctctcca tgacgaatta gaatgaggtt cattattttg
     4441 tctcccactg aataatatta acggaaaatg aagagttagg gagtaaattc aacctctaac
     4501 ccttctgttc ttgcttcttc taaaagttca ggaggcgggg gagttaaggg tgatgctcta
     4561 taaatagcct tgacacaaaa atcatcaaat gctgtatttc ctgatggtat atctacctta
     4621 acatcagatg gccttcctct ctcatcgagt ttgaaaacaa tcactgcctt tagggagcca
     4681 tcaataggaa tgttttcagg aattgcccaa ttgtttttta tttcctcacg aacccttctc
     4741 acaaatagct cagcgactac tcctgaagaa ccgccccttg atcctccagt tgaatagcca
     4801 gcacctttct gaaccccttt tctctctgcc gatgaatcag cactggcgac ccttttctgt
     4861 tgaaactctt taagaagttt ctgtttctgc atctctccga tcaccctatt ctttacgtct
     4921 tcaaatgtct cctttttact ctcaaccttt ttttgtggct ttgtcttagg aggttcagta
     4981 ggctccggtt ttttcgctat ttcttttttc tccttcttct tctccttctc ctgtaatact
     5041 attgtgtcat cttgcttagg tttctctttt ggagtttcct ctttttttaa ttcggttttt
     5101 tttggttctt cttgaggttc ctttttctct ggcttagaaa cttgaatctt ctcttcaggc
     5161 tttttttctt caacctttgc aggttcttcc tttgtctcag gtacttgtgg ctttacttcc
     5221 tctttctcgg tagcaacctc cccacccggt tgtccaagtt cggaaatgga gaccaaagtc
     5281 ccttctatgg actcaggttc ccctgagtac atgggggacc caaatcccca gaaaatagcc
     5341 attaaaaaaa tgacgatatg aagggaaagc gataggacta ccccccaaaa ccaagaaacc
     5401 cctgaaatcc catctttaga tgcactcatt ttttctgtct agtttgagaa ctattagatg
     5461 atagcggctc tgtaacaagc cctagttttt ttacacctgc cttttttatc tccgccatca
     5521 cctccatcac atccccgtaa ggaacatcgg catccgctct aagaaataca tctccatctt
     5581 ctattctttt c
//
LOCUS       DATDRL010000347        20162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1993260, whole genome shotgun sequence.
ACCESSION   DATDRL010000347 DATDRL010000000
VERSION     DATDRL010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 20162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20162
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993260"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..735
                     /gene="purN"
                     /locus_tag="VI935_11600"
     CDS             130..735
                     /gene="purN"
                     /locus_tag="VI935_11600"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013755986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HLE26271.1"
                     /translation="MNKKTRVGVFVSGSGSNLQALIDAKIPSVEIVVVVSNNPDAYAI
                     ERAKKDKIPVEVIDHRNYSSREDFEKEIQKRLDQYKIDLIALAGFMRILTPLFIRNYK
                     NRIMNLHPALLPSFPGTNAVKQALMYGVKFTGCTVHFVDEGVDTGPIILQTVVPINDT
                     DTEESLLERIHKEEHRIYPEAIRLFGEGKLRIEGRRVFIRD"
     gene            869..1123
                     /locus_tag="VI935_11605"
     CDS             869..1123
                     /locus_tag="VI935_11605"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HLE26272.1"
                     /translation="MNETVSEKKNTFVEVYRTEDVHLAGLIKEVLEGHEILCYLDNYY
                     SHSLNVHLFFPLAYHGIKVMVYEEDIIEAKEILSLFFKEK"
     gene            complement(1293..2312)
                     /gene="aroF"
                     /locus_tag="VI935_11610"
     CDS             complement(1293..2312)
                     /gene="aroF"
                     /locus_tag="VI935_11610"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HLE26273.1"
                     /translation="MIIVMRSDATKEEIEKVIATVKELGYKAHIIEGVLRTVIGAVGD
                     ERGKPHHLEAIGILSGVEKVVPILQPYKLASREFKSENTVVKVGNAGIGDNKVVIMAG
                     PCSVENEEQIIETSKAVKEAGAAVLRGGAYKPRTSPYSFQGLGEEGLKLLAQARKITG
                     LPIVTEILDPHDIDIVAEYSDMLQIGARNAQNYALLRYVGRCNKPVLLKRGMSTTINE
                     FLMAAEYILSEGNHNVVLCERGIRTFETATRNTFDLNAIPVLKEKTHLPVIADPSHGT
                     GYWQYVTPMALAAIAAGADGLMIEVHYKPEIAQSDGGQSLKPSKFKALMEKAKLVAKA
                     VGRNI"
     gene            2471..3070
                     /locus_tag="VI935_11615"
     CDS             2471..3070
                     /locus_tag="VI935_11615"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HLE26274.1"
                     /translation="MGRITFFVAITVVVISFLSLKVAHTQTMGEGTGVNPQGSQELPQ
                     GAETQPQAPNVIQDQPNYPYIDNSGRVYPYEDLNPFGLSTLGPGDDEYNTEGSTLGTD
                     IKRKSNLEVNQPRERKQPEATNEGETVSGGEETAVEGGEEAVEVIPEESSEVLTPSPL
                     GKTSGFYKWVDKDGVLHVTDNPGSIPQEYQGQLTNEPTK"
     gene            complement(3306..3563)
                     /locus_tag="VI935_11620"
     CDS             complement(3306..3563)
                     /locus_tag="VI935_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26275.1"
                     /translation="MRLARVAILPLVLALNNCSGQSEGAGYWFHFIFVVIPLIIIGFF
                     IFIKSEKVIDSLFTIEGQLKKLTNKVENLEEKLKKLTEKEQ"
     gene            3639..4364
                     /locus_tag="VI935_11625"
     CDS             3639..4364
                     /locus_tag="VI935_11625"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLE26276.1"
                     /translation="MLRACCCWASIIFFTIIFGIPATILSFIAPDKVHKYFIRPWSNL
                     ILRACNIKVQLEGLENVPKGPCLIMFNHQSYFDIFAFAATLPFDWRAVMKKELALVPF
                     VGWVAKATGHYFVSRDSSIRSFQEIDKVAKRIRLSPPVLIAPEGTRSLDGKLLSFKRG
                     GFVLAMRAEVPVVPMVILGGKDIMPKDSMTIKPGNMKINILPAIDVKSLPSGNLGRDE
                     LLRHVKETLEKALVEGERSEVAS"
     gene            complement(4821..5441)
                     /gene="trmB"
                     /locus_tag="VI935_11630"
     CDS             complement(4821..5441)
                     /gene="trmB"
                     /locus_tag="VI935_11630"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HLE26277.1"
                     /translation="MSQNISVSPHILDVTEYYLPIDLSQVFGNNQDSALEIGFGDGSF
                     LIETARNNTDWNFIGIEIKEKRFKKAVKKAEVENIANLKLLLMDVKIATDEVFCPNTF
                     SKVYINFPDPWPKERHKKHRIINTHFLEKLSRIMKPKGVLEIASDHEEYISYILETLD
                     GTGIFKPLFPSPGYVHSVSNRPKTRYEMEFMKEGKEICYLRFIKDK"
     gene            complement(5528..6631)
                     /gene="aroB"
                     /locus_tag="VI935_11635"
     CDS             complement(5528..6631)
                     /gene="aroB"
                     /locus_tag="VI935_11635"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HLE26278.1"
                     /translation="MKKIKVNLKKIEDRSYDILIGEDILKKIPLDLKKEKPAYSYVII
                     SDSVVASLYGQKLLRAFTSSGLNSHIVSFPAGELHKNRDTKARIEDEMSKLKIGRDSC
                     VIAFGGGVVGDVAGFVSATYNRGIPYIQVPTTLVAAVDSSIGGKTGVDTPHGKNLIGT
                     FYQPWRVYIDVITLRTLHEKEMREGLAEVIKYGVIRDEKLFEYVEKNIDHIFSFNSDA
                     LIHIIKSGCEIKGQVVELDEKESNLRKILNFGHTIGHAIENLSDYKISHGEAISIGMV
                     AEGKIALELGFWKLDEFERLVSLFKRTGLPTKPPDSLDLSIMINTMKLDKKARQGKIE
                     MVLPRKIGEMGEVDGNYGIRIEEKLMTRILGSN"
     gene            complement(6634..7596)
                     /locus_tag="VI935_11640"
     CDS             complement(6634..7596)
                     /locus_tag="VI935_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HLE26279.1"
                     /translation="MKPIGVIFNPLADINKKQTAEQLRSIKNILTKHAFIRITNNPNE
                     IPMALKEFHKDGIKILGISGGDGTIAHVLSSYINLFGSEDLPVIVPLKGGTMNILVAE
                     VGLTDDQIASCRKLTQYLNHQSKLPTIERGLIKVTDKKLDHIKYTFTWLDGLLYKFIK
                     WYNKEGGGVRVALKLILKSGIISLTNLNHDLFKEVGSKVYLDDKMLPFETHLLIAVST
                     VKRLVFGFRAFAEEAVAGERFSFFYLRLPYFRNALYQLPRVLYAGLKSDISGNFLNHS
                     GRIVRIEGNKGYVVDGEVYEFEEPTDITLEVGPKIKIFSIRDEK"
     gene            complement(7593..8114)
                     /locus_tag="VI935_11645"
     CDS             complement(7593..8114)
                     /locus_tag="VI935_11645"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HLE26280.1"
                     /translation="MEDLKKYIRDIHNFPKEGIIFHDITPLLQNGKAFGHAIDAMADM
                     IKGKSVEYLLGVEARGFIFASALAYKLNLGLVIVRKPGKLPYQTFNASYDLEYGKDML
                     EVHRDAISHGSRVILVDDLLATGGTAAAVGNMVRRLGGEIIGYCFLVELTELNGREKL
                     RPYPVWSVLEFPV"
     gene            complement(8332..8985)
                     /locus_tag="VI935_11650"
     CDS             complement(8332..8985)
                     /locus_tag="VI935_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HLE26281.1"
                     /translation="MIWLLVSLALGLSDACVKRKPITPRDLEDCIRKDHLFAEEQTYH
                     IVKEGETLYSISKTYGISLEEITEVNGIDDPSHVSVGLRLIVPGHKISDLIWPVKGKI
                     TSQFGSRGWIGFHSGIDISASKGTPVRAVADGLVTASAPSLDGYSGYGKVIIIEHGDG
                     IRTLYAHNKNNLVEIGQCIREGEIIAEVGSSGNASGSHLHFEIRKDGRPVNPLEYLP"
     gene            complement(8948..9409)
                     /locus_tag="VI935_11655"
     CDS             complement(8948..9409)
                     /locus_tag="VI935_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HLE26282.1"
                     /translation="MSTIIGVIGTSEASTEECKTAEEVGREIARRKGILVCGGLGGVM
                     EASCRGAKSEGGLTIGIIPGINRTDANHYVEVPIVTGISEARNIIVVRSSNAIIAIGG
                     SFGTLSEIAFALKLEIPVIGLNTWEVSTNIKKATTPKEAVDLAFSLARPRT"
     gene            complement(9683..10213)
                     /locus_tag="VI935_11660"
     CDS             complement(9683..10213)
                     /locus_tag="VI935_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLE26283.1"
                     /translation="MVRKIIKNGKIFYQYKGELYPEYLNQGNAQSFISEKALAYCDGT
                     GIDVGADRWPLAGAIPILDEATQNAYKLDNFQDDSLDYVFSSHCLEHLENWQDALGMW
                     IRKLRKGGIIFLYLPHESMKLWHRGGPWVGGHHKWRPNYQILIPFLQKHGVEILEFNP
                     FRDEYWSFHIVGKKSA"
     gene            complement(10601..11347)
                     /gene="surE"
                     /locus_tag="VI935_11665"
     CDS             complement(10601..11347)
                     /gene="surE"
                     /locus_tag="VI935_11665"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HLE26284.1"
                     /translation="MRILVSNDDGINSAGLDALADVLKTVGEVFIVAPDREQSAASHT
                     LSLQRPLRVEEISETIYSVDGTPTDCVNLAVNGLLKDKKPDLVVSGINKGENLGDDIT
                     YSGTVSAAMEGTLLGIPSIAVSLATKNNFYFETAAYYALLITKYVLRKGLLEGTLLNV
                     NIPNLPKEKVKGIKVTRQGKRVYGEPIVENIDPRGRKYYWIGGNELGFLDIENSDIIA
                     VNNGYVSVTPIKLDLTDYNFLDRLRLDLKI"
     gene            complement(11500..11575)
                     /locus_tag="VI935_11670"
     tRNA            complement(11500..11575)
                     /locus_tag="VI935_11670"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11540..11542),aa:Pro,seq:ggg)
     gene            complement(11623..11967)
                     /locus_tag="VI935_11675"
     CDS             complement(11623..11967)
                     /locus_tag="VI935_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HLE26285.1"
                     /translation="MFGDVAEVKLPDKIYFRIGEVSEYTGVQPYVLRYWESEFQEIQP
                     IRRKSQRLYDRETILIIMKIKHMLYEQNYTISGAKKKLKEEVNQRRVSTKVNELLREI
                     LGELKSIKQKIE"
     gene            complement(12414..13010)
                     /locus_tag="VI935_11680"
     CDS             complement(12414..13010)
                     /locus_tag="VI935_11680"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HLE26286.1"
                     /translation="MPDYSYERRIWGSGKLPAGVDEAGRGPLAGPVVAAAVILPKECE
                     INGLNDSKKLSSQKREFLFDQIIRLSVSIGVGIVEPGEIDRLNILRAALLAMENAVMK
                     LNPKPDFLLIDGNIETSLLIPQETVIGGDSTCNSIAAASIIAKVTRDLIMYDYHNIHP
                     EYNFKKHKGYPTKEHFEALKKFGPCPIHRRTFKGVIWK"
     gene            complement(13098..13973)
                     /gene="dapA"
                     /locus_tag="VI935_11685"
     CDS             complement(13098..13973)
                     /gene="dapA"
                     /locus_tag="VI935_11685"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HLE26287.1"
                     /translation="MIHGSIVAIVTPFKNGKVDVKSLQDLIEFQIENGTHGIVPCGTT
                     GESPTLSHEEHEFVIEVTVKTVEKRIPVIAGTGSNSTDEAIRLTKFAERIGVDAVLLV
                     VPYYNKPTQEGLYLHFKHIASQVKIPIILYNIPGRSGVNMNPDTIGRLANDCKNIIGL
                     KEASGSLQQASNILYLCGMDFLLLSGEDALNFPLLVIGGKGFITVTANVAPRDVAELY
                     NSYVRGEFNQARELHYKLFPLNEAMFLETNPIPVKVALALMGKIQNEFRLPLCQMSSG
                     NLEKLKTALKEYGLI"
     gene            complement(14338..14883)
                     /locus_tag="VI935_11690"
     CDS             complement(14338..14883)
                     /locus_tag="VI935_11690"
                     /inference="COORDINATES: protein motif:HMM:NF025825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HLE26288.1"
                     /translation="MFRIPLISKITHGLQNYLNKLDNQNITELPLGIKSGLTKYLTHD
                     EELLFTLRDFRAIYKAPRWIDSNTLFNSWFILTNKRIIIAKNSSSFKRFRDIPHNKIS
                     QIDYETGLLESRLIIHSPRTVDIIEFIGEAKKHSEGLDHKIHKTKEEAISDTVFCSRC
                     ESKMPKGSQFCSECGEKIGEA"
     gene            complement(14876..15898)
                     /locus_tag="VI935_11695"
     CDS             complement(14876..15898)
                     /locus_tag="VI935_11695"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HLE26289.1"
                     /translation="MKKESYNVAVAGATGAVGQEMISILEERNFPVGELRLIASEKSQ
                     GKDIEFNGRKIKVQRLDENSFKDVHIALFSAGASRSREFAPKAVQAGAIVIDNSSAFR
                     METDIPLVIPEINGHRISDYIKKGIIANPNCTTAVTVMALKPLHDISRITRVVAASYQ
                     AVSGAGAKAMEELRLQTLAWAKGERMKVEAFPHQIAFNLLPHIDTFLENGYTKEEMKL
                     HNETRKILEDGSIRVTGTTVRVPVFRAHSVAVNIETERKITAKEARKALSSFPGVKVV
                     DEPQNLKYPMPIDAAGKDDCLVGRIREDYTVPAGLSFWVVGDQLRKGAALNAIQIAEV
                     LIRDYV"
     gene            17915..19666
                     /locus_tag="VI935_11700"
     CDS             17915..19666
                     /locus_tag="VI935_11700"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HLE26290.1"
                     /translation="MTDKSDYIPGETVLITGSGWWPGETVALELVEEPLIHPAETLYA
                     IADADGNITNAEYVIQEHDLGQSFTLTATGQTSGLIAQTTFTDASLSAAPLLIKESTC
                     VTNTSTFTLGQTVCASTTVTVTGGGALPDVRIQWYNPSNTLVQDTLKSGVSDGSTQTD
                     TFIPNVTGTWTVKTCKSSGAGSCSVGNTMDTKTFAVVQCSTNADCNDSNVCTTDTCVS
                     NLCVFTNNTDSCDDGNACTTSDTCSGGSCGGTPITCNDDNICTDDSCDPATGCVYTPN
                     NSACDDSNACTESDTCSGGSCGGTPITCNDDNICTDDSCDPATGCVFTPGNAGTVCNP
                     GSGDLCDPDETCDGVSAVCPADTIAPATTICNPGSGDICDPDESCTGVADAACPTDNI
                     APATTVCNPGSGDICDPDESCTGVADAACPADVVAPPDTVCNPSINEFCDVDELCTGV
                     ADQACPDDVVKDYYFVGFLSPTDNPPVFNMGNAGRTYPIKWQLPDCEGGLPTGGFVSD
                     LDVVGGIDTYRVMCDASSPMDPMEAETSGNSGLHYDFVNNQFIFNWKTSRTFANKCYE
                     LRVTFDNDEMESAYFDF"
BASE COUNT         5550 a         4292 c         4029 g         6291 t
ORIGIN      
        1 gtagctgccc tgattctttt ttagttttgt agatttccta cctcagtaat taattttaca
       61 aaacaaattt agcacaataa atatttagta tctattaatt atagttattt attcttaaag
      121 taacgtttaa tgaataaaaa aacacgagtg ggtgtttttg tatcaggaag cggaagcaac
      181 cttcaagcct taatcgatgc taaaattcca agtgtagaga ttgttgtggt cgttagcaac
      241 aaccctgatg cttatgccat tgagagggca aaaaaagata aaatccctgt agaggttatt
      301 gaccacagaa actattcgtc aagggaggat tttgaaaaag agatacagaa aaggcttgac
      361 caatataaga ttgaccttat tgcacttgca ggcttcatga gaatccttac accactattt
      421 ataagaaact ataaaaatag aatcatgaac ttgcatcctg cgcttctccc ttcttttccg
      481 gggacaaacg ccgtcaagca ggcgctaatg tatggtgtga aattcacagg ttgtacagtg
      541 cattttgtag atgagggggt tgataccggt cccatcatac tccaaaccgt tgtaccaatt
      601 aacgatactg atactgaaga gagcctactt gaaagaatcc ataaggaaga acatagaatt
      661 tatccagaag caataaggct ttttggtgaa ggaaagttaa ggattgaagg aagaagggtc
      721 tttatcaggg attaggaatt aggaattaat tacaataccg aattcctaat tcccaattcc
      781 taaaatgggt caatgtccaa tctgtaagaa gaaatattct agagatgtag aatattgtcc
      841 tagatgtcag gttggaataa ttccttcttt gaatgagacc gtaagtgaaa agaaaaatac
      901 gtttgttgag gtttatagaa ctgaagatgt gcatcttgct ggtttaatta aagaggttct
      961 tgaaggccat gaaatacttt gttacctgga taattattat tcacacagcc ttaatgtgca
     1021 tcttttcttt ccactcgctt atcacggaat aaaggtcatg gtttacgaag aggatattat
     1081 agaggctaaa gagattctta gtcttttttt taaagaaaaa taattacttt gccgctaagg
     1141 tgcaaatgag ggatatgagt ttaaaaagtc ggttaagaac caaggagaca gaattcagga
     1201 gttagaattt tggggtcttc ttctgtatcc tgccttctag attataacct agggttttaa
     1261 tggtttttgg ctgaagaata gttacaagaa aattaaatat tccttcctac cgccttagca
     1321 actagttttg ccttttccat aagtgcttta aactttgatg gctttaatga ctgcccaccg
     1381 tcactttgag cgatttcagg tttatagtgc acctcaatca ttagtccatc agccccagca
     1441 gcaattgctg caagtgccat tggcgttaca tactgccagt aaccagttcc gtgacttggg
     1501 tcggctataa caggaaggtg tgttttttcc ttaagcactg gaatcgcgtt taggtcaaac
     1561 gtgtttcttg tggcagtttc aaaggttctt atacctcgct cgcatagaac gacattgtga
     1621 ttaccttcag aaagaatata ctctgcagcc atgaggaatt catttatagt tgtggacatc
     1681 ccgcgcttaa gaagcactgg tttattacat cttccaacat atctaagaag tgcatagttc
     1741 tgagcgttcc tagcccctat ctgtagcatg tcagaatact ctgctacaat atctatgtca
     1801 tgcggatcga gaatctcagt gactatagga aggccagtaa tttttctggc ctgggcaagc
     1861 aattttaatc cctcctcgcc aagcccctgg aaggagtaag gagaagttct tggcttatat
     1921 gcccctcctc ttaagacagc tgcgccagcc tcctttactg cctttgaggt ttcaataatc
     1981 tgctcttcat tctcaaccga acatggtcct gccataatga caaccttgtt atctccaatt
     2041 cctgcgtttc caactttaac tactgtgttt tcagacttaa attcacgact agcgagttta
     2101 tagggttgaa gtattggaac tactttttca acacctgaaa gtataccaat ggcttcaagg
     2161 tggtgtggtt ttcctctttc atccccaact gctcctatga ctgttcgcaa gacaccttca
     2221 atgatatgag ccttataacc gagttcttta actgttgcta ttactttttc aatctcttct
     2281 ttagtagcgt cgcttctcat tacgataatc ataaaacttc ctccgctcaa tgttccgctc
     2341 ttttttgtag ataaattccc atattacatg aagcgttttt attgtcaaat attgtcaaat
     2401 ttgggtattg agttatagaa tttggagttg tataattaat gatgcttatt ataggcaagg
     2461 agggataaaa atgggtcgaa taacattttt tgtagccata accgtagttg ttatatcttt
     2521 cttgtcacta aaggttgctc atacacaaac aatgggtgaa gggaccgggg ttaatcccca
     2581 aggctctcag gaactgcctc agggagctga aactcaacct caggcaccca atgtgattca
     2641 ggatcaaccg aattatcctt atatcgataa ctctggtagg gtttaccctt atgaggattt
     2701 aaaccctttt ggcttatcta ctcttggtcc aggagatgat gaatataata ccgagggaag
     2761 cacgctcggc acagacataa aaaggaaaag caatctagag gttaatcaac caagagagag
     2821 aaagcagcct gaagcaacca acgaggggga gacagtatct ggtggggaag agactgcagt
     2881 tgaaggtggt gaggaagctg tcgaggttat tccagaggaa tcgagtgagg tactaacacc
     2941 ctcgccgtta ggtaaaacta gtgggtttta taagtgggtg gataaagatg gggtcttaca
     3001 tgtaacagac aaccctggtt ctattcctca agagtaccaa gggcagctta caaatgagcc
     3061 tacaaagtga gaggttaggg aggtaatcaa ccttagggga ttttaactga taagggcttc
     3121 aactcgataa atcactgttt actacagggt ttcatgtcat tgcgtgcaca ttcgcggagc
     3181 ctgtactgag gaaatcgaag tgctcagggt aaactccgtt aagcaattta acacaagttg
     3241 tcattccgaa cattgtgagg aatctcaaac agcttactgc agattattta attttaatta
     3301 tatgttcact gttctttttc agtaagcttt tttaattttt cttctaggtt ttctacttta
     3361 ttcgtaagct ttttaagctg tccttcaatt gtaaaaagcg agtctattac tttttcactt
     3421 tttataaaaa taaaaaaacc aattataatt aatggtatca ctacaaatat aaagtggaac
     3481 caatacccag ctccttctga ttgacccgag cagttgttaa gtgctaagac aaggggtagt
     3541 atggcaactc ttgctaacct catctaatta atcctcctaa atgcattgtt cttagacgtt
     3601 gaaacgtatc gacttaaata atataataaa ctataaaaat gttaagggct tgctgctgct
     3661 gggcatcaat tatttttttt acgattattt ttgggattcc agctaccata ttatccttta
     3721 tcgctcctga taaagtgcat aaatacttta taaggccctg gtctaatttg attctcagag
     3781 cctgcaatat taaggtacag ttggaagggt tggagaacgt tccaaaagga ccttgtttga
     3841 taatgttcaa ccaccaaagc tattttgaca tatttgcttt tgccgcgact cttccctttg
     3901 attggagagc agtgatgaaa aaagagcttg cacttgttcc tttcgtcggt tgggttgcaa
     3961 aggcaacagg gcattatttt gtttcaagag atagctctat aaggtctttt caagagattg
     4021 acaaggttgc aaaaaggatt cgccttagtc ctccagtttt gattgctcca gagggaacaa
     4081 gaagccttga tggaaagcta ctttcattta aaaggggagg gtttgttctt gctatgcgtg
     4141 ctgaggtgcc tgtagttcca atggtgattt tagggggaaa agatattatg ccaaaggact
     4201 caatgacaat taaacctgga aatatgaaga ttaacattct tcctgcaata gatgtgaaat
     4261 cgcttccatc aggcaacctt ggaagagatg agcttttaag acatgttaag gagactttgg
     4321 agaaagcttt agtcgagggt gaaagaagcg aggttgcatc ataatttttg tgataggttg
     4381 tgattgagtt ataaatattc caattctaaa ttaagtcgct atttatttta acttgataat
     4441 tccctgcaac ctgctacagt gttttctttg acatagcgag ccgaaggcga agcaatctct
     4501 tatttagatt gcttcggccg tttcctttta tttccatttc tcgcacaccg cgctcgaaat
     4561 aattctcctc gctgacattc ctcgttgtta ttgctttgct cagagtttcg gccgactcgc
     4621 tcctagcaat gatctaagcg gttgtctgtt tatacaccga taccttgcag cttggaggtt
     4681 gtccgataaa tgcaaaaaac ggcattcttg ggggtagcaa aacccctccc aggttgccga
     4741 tcgttgatcc tggggcattc tgaggggtca aatttttacc catccataat tgtcggacag
     4801 cctccttgca gaagggtttt tcatttgtct ttaataaacc ttaggtagca aatctccttt
     4861 ccctctttca taaattccat ttcatacctt gtcttaggtc tatttgatac actgtgaaca
     4921 taacctggtg atggaaataa gggcttaaaa atacccgtgc catctaaggt ttctaatata
     4981 tatgagatat attcttcatg gtcgctcgct atctctagca caccttttgg tttcattatc
     5041 ctagagagct tttccagaaa atgagtgttt atgatcctgt gctttttgtg acgttcttta
     5101 ggccacgggt ctgggaaatt tatataaacc ttgctgaatg tgtttggaca aaaaacctca
     5161 tcagtagcaa ttttaacatc cataaggagt aattttagat tagcgatatt ttcaacctcg
     5221 gcctttttta ctgctttttt aaatcgtttc tcctttattt caattcctat aaagttccaa
     5281 tctgtattat tccttgcagt ctctattaag aatgatccgt ctccaaaccc aatttcgagc
     5341 gcagaatctt ggttgttacc aaagacctgt gataaatcga taggtaggta gtattctgtc
     5401 acatccagta tgtgcggtga tacactaata ttttgtgaca tttctgattt aaagggaatg
     5461 tgcttattct ttttagtcta aacctttttt cagtttaaaa ctttataatg cttttcagga
     5521 ggattaacta gttcgatcca agaattcttg tcattaattt ctcttcaatc cttatcccat
     5581 agtttccgtc aacttctccc atctcaccga ttttccttgg aagaaccatc tctatttttc
     5641 cctgtctggc tttcttatca agcttcattg tgttaatcat tatgcttaga tcaagggaat
     5701 cggggggctt tgtaggaagt ccagttcttt taaataagga aactaacctc tcaaactcat
     5761 ctaatttcca gaatccgagt tcaagagcaa ttttaccttc tgccaccatc cctattgata
     5821 tcgcttcgcc gtgggatatt ttgtaatcag aaagattctc aatcgcatga ccgattgtgt
     5881 gcccgaagtt gagtattttt ctcagattcg attccttctc atcaagttct actacctgtc
     5941 cttttatttc acaaccactt tttattatgt gtataagggc atcggaatta aaagaaaata
     6001 tatgatcaat atttttttcg acatactcaa aaagcttctc atccctaatt accccatact
     6061 ttataacctc agcaaggcct tctctcatct ccttttcgtg taatgttctt aatgtaataa
     6121 catctatata cactctccaa ggttggtaaa atgtgccgat caggttttta ccatgcggag
     6181 tgtcaacacc tgttttgccc ccaattgagc tatcaactgc tgctacaagt gtagtgggaa
     6241 cttgaatata ggggatccct cgattgtacg ttgctgatac aaaccctgct acatctccta
     6301 cgacaccacc accaaaagcg attacacatg agtctcttcc aatcttgagt tttgacattt
     6361 catcttcgat tctagctttg gtatctctat tcttgtgtaa ctctcctgca gggaaagaaa
     6421 caatatgcga attgagtcct gaacttgtaa acgctctaag gagtttttgc ccataaagag
     6481 atgcgacgac agaatcagat attatcacat acgaataagc gggtttttct ttttttaagt
     6541 ctaggggtat tttcttaaga atatcctctc caattagaat atcgtatgat ctatcctcta
     6601 ttttctttag gtttactttt atctttttca ttattatttc tcgtctctaa tagaaaagat
     6661 ttttatctta gggcctactt ctagggttat gtctgttggt tcttcaaatt catagacctc
     6721 tccatctacg acatatccct tatttccttc tattctcaca attctgcctg agtggtttaa
     6781 gaaattacca gaaatgtcgg actttagccc agcgtataat acccttggaa gctgatagag
     6841 agcattcctg aaatatggaa gccttaaata aaaaaaacta aatctttctc ctgctactgc
     6901 ctcttcggcg aaggccctga aaccaaaaac cagtctcttt acagtagata ccgctatgag
     6961 caggtgggtt tcaaagggaa gcattttatc atcaagatag acctttgatc ctacctcttt
     7021 aaaaaggtcg tggttaaggt ttgtcagcga gattatccct gatttaagaa tcagttttag
     7081 tgcaacccta acgccccctc cttctttgtt gtaccattta ataaatttgt aaagaaggcc
     7141 gtcaagccat gtgaacgtgt atttgatatg atctagcttc ttatcagtta ctttaataag
     7201 ccctcgttct attgtaggaa gtttggattg gtgattgaga tattgagtta acttacggca
     7261 ggaagctatt tgatcatctg taagaccaac ttcagctaca agtatattca ttgtaccacc
     7321 ttttaggggg acgattactg gtaagtcttc acttccaaag agatttatgt aagaggacaa
     7381 tacatgggca attgtcccgt ctcctccgct aatgccgagt atctttatac cgtctttatg
     7441 aaattctttg agagccatcg ggatttcatt tgggttattt gttatgcgga taaatgcatg
     7501 tttagtgaga atattcttta tcgaacgtag ctgctccgcc gtttgcttct tatttatatc
     7561 agctaatgga ttgaatatga ctccaatggg tttcatacgg gaaattctaa cacagaccag
     7621 acagggtagg gtctcagctt ttctctacca tttagctcag tgagctctac aagaaagcag
     7681 taccctatga tttctccacc gagccttctc accatattac caactgccgc cgcggttcct
     7741 cctgttgcaa gcaggtcatc taccaaaatg actctgctgc cgtgtgagat ggcgtccctg
     7801 tgtacttcaa gcatatcttt tccatattct aggtcgtagg aagcgttgaa ggtttgataa
     7861 ggcagtttcc ccggttttct tacaatcaca agcccaagat taagtttgta agctagtgct
     7921 gaggcaaaga taaatcccct tgcctcaacc cctaaaagat actccacact cttaccctta
     7981 atcatatcag ccatcgcatc tattgcatgt ccaaaggctt ttccgttttg aagaagaggg
     8041 gttatatcat gaaatataat tccctcctta gggaaattat gtatgtctct aatgtatttc
     8101 ttaaggtctt ccatgatgtt tcctcctcga tagtttcttg ttcagccatc atatagaaaa
     8161 gattgtacgc gaatgaatca tgcgtgattt atgatgcacg atgcatgata catgatgaat
     8221 gaagttttac tgtatctttt atcatgcatc ctgcatcttg tatcttgctt atttgtgacc
     8281 atcgttcgac tgagctcatg atgaagtctg agtgaattcg cggctgaaaa gtcacggcag
     8341 gtattctaac ggatttaccg gccttccatc ttttctgatc tcaaaatgaa gatgcgaacc
     8401 gctagcattc ccacttgagc ctacctcggc aataatctca ccttctctta tacattgtcc
     8461 tatctctact aagttatttt tgttgtgcgc ataaagcgtt cttatcccat caccatgttc
     8521 tataattata accttcccgt atcctgaata accgtcgaga ctaggtgcgc ttgctgttac
     8581 tagcccatcc gctacagccc tgactggagt gcctttagaa gctgatatat ctattcctga
     8641 atgaaaacca atccatcctc tacttccaaa ttgcgaggta attttgcctt tcacaggcca
     8701 gattaaatct gagatcttat gccctggaac aattaatctc agtccaactg aaacatgcga
     8761 cggatcgtct ataccattga cctctgtgat ttcttccaga ctaatgccgt atgtctttga
     8821 tatactgtaa agggtttctc cctctttgac tatatgataa gtctgctctt cggcaaatag
     8881 atgatcttta cgtatgcagt cctctaagtc ccttggagta ataggcttgc gttttacaca
     8941 agcatcgcta agtccgaggg cgagcgagac taaaagccaa atcaactgcc tcttttggag
     9001 ttgtggcttt tttaatattg gttgatacct cccaagtatt taatccaatt acagggattt
     9061 ctaacttaag agcaaaggca atttccgaga gtgttccaaa actacctcct attgcaatta
     9121 tagcattgct tgaacggacg acaataatat ttctcgcctc gcttattccg gtaactatag
     9181 gaacttctac ataatgatta gcgtcagtcc tgttaatacc tggaattatt cctatagtta
     9241 gccccccctc tgattttgct cctcggcatg aagcctccat tactcccccg agtcctccgc
     9301 aaacaagaat gccctttctt ctagctattt ctctccctac ttcttctgcc gttttacatt
     9361 cttcagttga agcctctgat gtgccaataa cgcctattat agtgctcatc aagctttata
     9421 tattttatca gattttgtaa caaattaacc actaatttac actaataaaa tgatgcacga
     9481 ttcatgatgc atgattcatc atttatcctg catcttgcat catgtatcct gtatcgtgta
     9541 ccatcctgca tctcgcttat tttcattcgt gtccatttgt ggttaatcaa aatcgaaact
     9601 cattcagatg tgaaacgctt ctactaactt tctttcgcta agctataaac aggcttttgt
     9661 tattttcgta cggagcgcat gcttaagcag atttcttgcc cactatatga aagctccagt
     9721 attcgtctcg aaatggattg aattcgagga tctctacacc atgcttctgc aaaaagggta
     9781 ttagaatctg atagttaggt cgccatttat gatgcccccc tacccaagga ccacctcggt
     9841 gccatagttt cattgactcg tgaggcagat aaagaaaaat gatacctccc ttccttaatt
     9901 tcctaatcca cataccaagt gcatcctgcc aattttccag gtgttctaag caatgtgagc
     9961 taaacacata atccaaacta tcatcttgaa aattgtcaag cttatacgca ttctgagttg
    10021 cttcatcgag tatcggaatg gcacctgcta agggccatct atcagcgccg acatcaattc
    10081 cagtgccatc gcaataagcc agtgcttttt ctgaaatgaa cgactgagcg tttccctgat
    10141 taagatattc agggtatagc tctcctttgt attggtaaaa gatcttgccg tttttgatga
    10201 tcttgcgaac cattagtcga cgtaagccac ccaacatatg cgggatgagt agcctcggat
    10261 ctgctaatac ctgcataatt tgagttatca tagtaataga atcttttaat tatattatag
    10321 atattttaaa agagttaaaa ttaaaagcta atataaactc gtaaccaaaa tgatatataa
    10381 agcctcgggg ttcgattaaa gcttttaact caaaactgac cgaaagtctc gaaactttaa
    10441 cccgatagct gagagccgat aggctgacag atttatttgg tagtacttac ttaacccatt
    10501 actttaccgc aataaaccct aagaacgaga agcacaaaaa agagggtaac aagaagggta
    10561 agctgtttat gtatgagtca tggggttttc tagacatgtt ctaaattttt aaatccagcc
    10621 ttaatctatc gagaaaatta tagtccgtca ggtctagctt tattggtgta acagaaacgt
    10681 agccattatt cacggcaatt atatcagaat tttcaatatc taagaaacca agttcattcc
    10741 cgccaatcca atagtacttt cttcccctag ggtcaatatt ttctactata ggctcaccat
    10801 atacacgctt tccctgtctt gtcactttta tgcccttaac cttctcttta ggaagattag
    10861 ggatatttac attaagcaat gttccttcga gtagcccttt tcttagtaca tatttggtta
    10921 ttaaaagagc ataataagcg gcagtttcaa agtaaaaatt gttcttagtt gctaacgaca
    10981 ctgcgattga aggaattccc aggagagtac cctccatagc tgcgcttact gtgcctgagt
    11041 atgttatatc atctcctaaa ttctcaccct tatttatgcc tgacactacc aaatctggtt
    11101 ttttgtcttt tagaagccca ttcaccgcga ggtttacaca atctgtaggt gtaccgtcaa
    11161 cgctgtatat agtttctgag atttcttcta cacgaagagg gcgttgaagg ctgagtgtgt
    11221 ggctcgctgc gctttgctcg cggtctggag caactataaa cacctcgccc accgttttta
    11281 gtacatcagc gagtgcatca agcccagctg aatttattcc atcgtcattt gatacaagaa
    11341 ttcttataga catataacta gtgagccatt aataaaatga tgcacgatgg atgataaatc
    11401 tttcatcctg catcttgcat tatgtatctt acacctgttt atacgttcgt gttcatttgt
    11461 gtcaattatt ggctaaattc ttacatcggc ataaaaaaat ggtcggggtg accggactcg
    11521 aaccggtgac caccggcccc ccatgccggt gcgctaccaa ctgcgctaca ccccgttacc
    11581 caggatttaa tttaggggga gttaatatgt gaattcggaa tattattcaa tcttttgctt
    11641 tattgatttc aactcaccta agatctcacg taataattcg tttacctttg tgctgacacg
    11701 cctttggttt acttcttcct ttagtttctt ttttgcgcca gatattgtgt aattctgttc
    11761 gtaaagcata tgcttaattt tcataattat aaggattgtt tctctatcgt aaagcctttg
    11821 tgactttctc cttatcggtt ggatttcctg aaattcgctt tcccagtatc taagcacata
    11881 gggttgaact cctgtatact cgcttacctc tccgattcta aaatagatct tatcgggaag
    11941 cttgacctca gccacatcgc caaacatggg atacaaacta gattatatct aaaaaaatgt
    12001 caaggatttg aaaagtaaaa ttcctcagga taggcttact ggtggtcagt cactggtcac
    12061 ggccaagcct atccagagct tagtcacggg gactcttgac ctaaacaaca tagacggaca
    12121 taaaacaaaa actggggtag cctaccagtc ttgctggtag gcgtcgcagc caacgagcaa
    12181 aaatgtgggg ttgttcgact gtgctaaaga agaagtcttt agaccaccat tatttgtctc
    12241 aggtgtcata cccgaatgtt tcccgctaga gacatgcggg aacaagattg atcgggtatc
    12301 cactcaaaaa gttattgatt cccgcttaca atccgcggga atgacaaatg ccgatgtaat
    12361 cataacctgt cctcagccag gtccccgatt taatcggaga aggaaggatt ttgctacttc
    12421 catattacac ccttgaacgt cctcctatgt atcggacagg gaccgaattt tttgagggct
    12481 tcgaagtgtt ctttcgtggg ataccctttg tgttttttga agttgtattc ggggtggata
    12541 ttgtggtaat catacattat taaatccctt gtaactttgg caataataga agctgctgca
    12601 atagaattac aagtggaatc accgcctata accgtttcct ggggaattaa aagagaggtt
    12661 tctatatttc cgtcaataag aaggaagtca ggctttgggt ttaatttcat aactgcgttc
    12721 tccattgcga gtagcgccgc cctgagaata tttaatctat caatctcacc tggctcgact
    12781 attcctactc caattgatac tgaaagacgt atgatctgat caaagaggaa ttctcttttt
    12841 tgagaagaaa gtttcttaga gtcatttaaa ccattaattt cacattcttt gggaagtatt
    12901 acggcagcgg ctactacagg ccctgcaagc gggccccttc ctgcttcatc aacacccgca
    12961 gggagtttcc cacttcccca aattcttctt tcatacgaat agtccggcat actcatttgt
    13021 gattcgggtt tcgtgactcg tggttggata cccaattcat gattccgatt cacgaaccac
    13081 gagccactat tcacgaatta tatcagtccg tattctttta atgcagtttt aagtttttcg
    13141 agattcccag atgacatctg gcatagagga agtctaaatt cattttgaat ctttcccatt
    13201 agagctaagg ctacttttac tggtatggga tttgtttcaa gaaacatcgc ttcattcagg
    13261 ggaaagagtt tatagtgaag ttctcgtgct tgattaaatt ctcctctcac atacgagtta
    13321 tagagttcag ctacgtccct aggcgctacg tttgccgtca cagttataaa ccctttacct
    13381 cctatgacta gtagaggaaa gttaagggca tcttctcctg aaaggagaag aaaatccata
    13441 ccgcacaggt agagaatatt gcttgcctgt tgaagcgagc ctgaggcctc ttttaaaccg
    13501 attatatttt tacaatcatt tgcaagtctt cctatggtat ctggattcat attaactcca
    13561 gaccttcctg gaatgttgta aagaataatc ggtattttta cctgagacgc tatatgcttg
    13621 aagtgcagat aaagcccttc ctgagtgggt ttattatagt atggaacaac aagaagaaca
    13681 gcatctactc caattctttc agcaaacttt gtaagcctta tggcttcatc agtgctgttt
    13741 gaaccggtcc cagctataac tgggattctc ttttccacgg tcttaactgt tacttcaatc
    13801 acaaactcat gttcttcgtg tgagagggta ggggactcgc cggttgtgcc gcagggaact
    13861 atcccatgtg tcccattttc tatctggaat tcaattaggt cttgaagaga ctttacatct
    13921 acttttccat tcttaaatgg ggttacaatt gctacgatgg agccgtgtat cataaagcaa
    13981 gcctccttcg ccaaaagagg ttgtcattgc gagcgctggc aaagcgatcc aacgcttctc
    14041 tgtcattgcg aacgaagtga agcaatcctt tgagattcct cactccgttc ggaatgacac
    14101 cctgtgtcgg attgcttcgt cgcggagcct gcgctgagcg catgcgaagt gctcctcgca
    14161 atgacaggtc tttcttagca gtatcagata aatgaattgg catcacacca ctttttcttt
    14221 ccattatatt agagtgtaag tgaaaatcaa ggactttatt aggaacgcct cacgccttac
    14281 cctcttacgt ctcacgccct cacgtctcac gctcccacgc ctcactctct cacgctgtca
    14341 cgcctcacca atcttctcgc cgcattcaga acagaattga cttcctttag gcatcttact
    14401 ttcgcatcta gaacaaaaaa ccgtatcgga aatagcctct tccttagtct tgtgaatttt
    14461 gtggtctaaa ccctctgagt gtttctttgc ctcacctatg aattcaatta tatctactgt
    14521 gcgaggtgaa tggattatta atctagattc caataaccct gtctcataat caatctggct
    14581 gatcttatta tgtggaatat ctctaaacct tttaaaagaa gatgagttct ttgcaataat
    14641 tattctcttg tttgtaagta taaaccagga attaaataac gtattgctat ctatccacct
    14701 tggcgccttg taaattgccc tgaaatccct aagagtgaat agtagctctt cgtcgtgtgt
    14761 taggtatttt gttaaaccgg attttatgcc taaaggcagt tcagtgatat tctgattgtc
    14821 gagtttatta aggtagttct gtagtccatg agtaatcttg gatatgagtg gaattctaaa
    14881 cataatctct tataagaacc tctgctattt gtattgcatt taatgccgca ccctttctaa
    14941 gctggtcacc cactacccag aagcttagcc ctgcaggaac ggtgtagtct tccctaatcc
    15001 ttcctacaag gcaatcgtcc tttcctgcgg catctatagg catcgggtac ttcaaattct
    15061 gaggctcgtc aactacttta acaccgggga aactgctaag cgcttttcga gcttccttgg
    15121 ctgtgatttt tctttcagtc tcaatgttaa ctgctacgga gtgcgcgcga aatacaggaa
    15181 cccttactgt tgtcccggtt acccgaatag acccatcttc taggattttc cttgtttcat
    15241 tatgcagctt catctcctct tttgtgtaac cgttttcaag aaaggtatca atatgtggaa
    15301 gaagattgaa ggctatctga tgtggaaatg cctcgacctt catcctctct ccttttgccc
    15361 aggcaagggt ttgtaaccgg agttcttcca tagccttagc cccggcgcca gagacggctt
    15421 gataacttgc agcgacgacc cttgttatcc gggatatatc atgaagaggc tttagcgcca
    15481 ttacggttac tgccgtagtg cagttaggat ttgcgattat acctttcttg atatagtcgg
    15541 agattctatg accgttaatt tcagggataa caagtggaat atcagtctcc atgcggaatg
    15601 cagagctatt gtcaatgaca attgccccag cttgaaccgc ctttggagcg aactccctac
    15661 ttctcgatgc tccagcagaa aagagggcga tgtgaacgtc cttaaaagaa ttttcgtcaa
    15721 gcctttggac ttttattttt ctaccattga attcaatatc ctttccttgt gatttttcag
    15781 atgcgatgag cctcaattct ccgacaggga aatttctctc ttcaagtatg ctaatcattt
    15841 cctgcccaac agcccctgtc gctcctgcca cagcaacgtt gtaactttcc ttcttcatct
    15901 ttcctccaaa tggatttaag aactgacaca gtattttaat tcataatgtg cttatagcca
    15961 aatttgaaag acacccaact taatcctccc ccaaaagtgg gggaggaaaa tactttcctt
    16021 cccctttgaa ggggaaggtt aggatggagt tcaaacaggg acctaggtaa cagaatagac
    16081 cgagtacatg ggttacataa tattacatgt catataggtg atctatggca tggaaagaag
    16141 aatggatttt ggaaacctaa aggtttccct gctggactgg aaagtccagc ctatccaata
    16201 taggtaacat acaagcggta gtcggggttt tctaaccccg cattgggtaa aatcaaaata
    16261 caccgataga cgatgttctc taaccttgac tccacccatc attgcaattg tggttgtagc
    16321 ggaaggcttt agccttccaa tcctcatatg gagacttgaa ggtctccgtt acattgagaa
    16381 taaacataat acgcaactcg tagtagaaat gttttctatc atcctggttt tgtcgaagga
    16441 tggcgtaatc gggaagcgcc tactatgttg tcatacccga acgcttttat cgggtatcca
    16501 catcgagtaa atactggatt tccaattaag ctcgtaccca catactttca gttggtaaca
    16561 tttgggaatg acaaataacc atagaagtct aagggtttcc tttaatgctc acctcgggac
    16621 tggaaagtcc cgactatgga aggaaaaatc accgcgtgca ataggcggtg ttttccaacc
    16681 cgacattggg tgcaatcaaa atacgccgat agacgaggtt ttctaacctg gtgatgtgaa
    16741 gtgggtcccc gacaaactca tcggggagaa agtccagcct atccaacata ggtaatatac
    16801 aaacgatagt cggggttttc taaccccgca ttgagtaaaa tcaaaatacc ccgatagacg
    16861 aggttttcta acctcgtaat gtgaagcggg actagaaagg cccgcctatc gtgtttggtg
    16921 aaataacaat tcctcgaagg cgggggtttc ctaaccccac attggtaaaa tcaaaaatac
    16981 cccgatagac ggggttttct aaccccgtaa tgcgaagcgg gactagaaag gcccgcctac
    17041 cccacatgga taactgacta tattgcgcaa tttttgtcac acttttgaca taaattgtca
    17101 ctatttcggt atgtaatctg gaaagagaaa aaatatcttt gttatatcaa tgagttatgt
    17161 ttttgaatca aacctcattt ttatggcata aaaattgcaa cataaaataa tgtttgacat
    17221 ttgttgggat tcaaatgtaa gattccttac aattaggatg gaggataact tatactaact
    17281 ccaaaaaatc tttttgccta ctgtattagc cctagaagta taagcttctc gaaagaaaag
    17341 gtgtatcaaa ttctttatga gaaaatttcc agctcaggag ttgagtccat cccctgggtt
    17401 ttggttgtat ccaattaata tacttaaagg aggagcttaa atgaagagta tactatccag
    17461 taaatggggg aagatggaga tatgtgcagg attatttggt ttaattggtt ttcttgcagt
    17521 ctttcttgct ttaacaataa gtaataccaa attcttaaat cacatcgtac aagctcaaga
    17581 aaatgccgct accgtgacga ctaatcaagc ggactattct gctgatgata tggctctaat
    17641 tacaggctct ggctggcaac caggagaaac ggtcaccctt gagattgttg aagaccctgt
    17701 cattcatgat ccggatacgt tatatgcggt tgctgacgct gatgggaata tttccaatga
    17761 gtacatcatt catggtcatg acgttgtaca gacgtttacg ctaacggcat caggtatggc
    17821 ccctaacgta gggttgcaga ctaccttcac gaacagttta aatcctacct cgaccgcctc
    17881 gctgccaacg acgcccacga cggatgctac ggtgatgacc gataaaagtg actatatacc
    17941 tggtgagacg gtgcttatta ctggttccgg ctggtggcca ggggaaacgg ttgctttgga
    18001 gctagtagaa gaaccgctga tccatccagc ggagacccta tatgcaatag ccgatgctga
    18061 tgggaatatt accaatgcag agtacgtcat tcaggaacat gacctaggac agtcgtttac
    18121 gctgacggca acagggcaga cttctggctt gattgcgcag acgacgttta cggatgccag
    18181 tttgtccgct gcacctttgt tgattaagga gtcgacttgc gtcacgaata cgtccacttt
    18241 caccttaggt cagaccgtct gcgccagcac aacagtaact gttacaggag ggggtgctct
    18301 gccagacgta cgtatacaat ggtataatcc aagcaacaca ttagtgcaag atacgctgaa
    18361 gtcaggagtc tccgacggtt ccacacaaac agataccttt attcctaacg tgactggtac
    18421 ttggactgtg aagacctgta agtcctcggg ggcggggtct tgcagtgtgg gaaatacgat
    18481 ggatactaag acgtttgccg tcgtgcaatg ttccacaaac gctgattgta acgacagcaa
    18541 cgtctgtacg actgacacat gtgtttctaa cttatgtgtt ttcactaata acaccgactc
    18601 ttgcgacgat ggcaatgcct gtactacttc tgacacctgc tcgggtgggt cgtgtggtgg
    18661 tacgccgatt acctgtaatg acgataacat ttgcacggac gactcttgcg acccggccac
    18721 tggctgtgtg tacacaccca acaacagtgc ctgcgacgac agcaatgcat gtaccgagtc
    18781 tgacacctgc tcgggtgggt cgtgtggcgg tacgccgatt acctgtaatg acgataacat
    18841 ttgcacggac gactcttgcg acccggccac tggctgtgta ttcactcctg ggaatgctgg
    18901 aacggtgtgt aatcctggtt ctggggattt atgtgatcca gatgagacat gtgatggagt
    18961 aagtgcagta tgtccggctg acacaatagc gccggcgacg acgatatgta atcctggttc
    19021 tggggacata tgcgacccgg atgaatcctg cacaggcgtt gcagatgccg catgtccgac
    19081 tgacaacata gcgccagcca caacggtttg taacccaggt tctggggaca tatgcgaccc
    19141 ggatgaatcc tgcacaggcg ttgcagatgc cgcatgtccg gctgatgtag tagcaccacc
    19201 agacactgta tgcaatccca gtataaatga attttgtgac gtggatgagt tatgcactgg
    19261 agtagcggat caggcatgtc cagatgatgt agtaaaagat tattacttcg taggattctt
    19321 gtctccaact gataacccac ctgtcttcaa tatgggaaat gccgggcgca cgtacccaat
    19381 aaaatggcag ttgccggatt gtgaaggtgg gcttcccacc ggtgggtttg tcagcgacct
    19441 ggacgtggtg ggtgggatag atacatatag ggttatgtgt gatgcctcat caccgatgga
    19501 tccaatggaa gcagaaacta gcggtaattc tggtctacac tacgatttcg ttaacaatca
    19561 atttatcttc aactggaaga ccagcaggac attcgcgaat aagtgctatg aactacgcgt
    19621 gacttttgac aacgatgaaa tggagagtgc gtatttcgac ttctagaaga gagaaaggct
    19681 gattagttat taaggcatta taaataaatt agggtgctga gcttgaaata atagagccag
    19741 cacccttttt tatcattccc gattaataat tatggcaaat tatcactgtc atccccgaat
    19801 gcatttatcg gggatccata aatccctgga ttccctataa ctacgttagg gaatgacagg
    19861 cggaggcgga ttcccgctta aacttgtact cgaatttctt tatcgggtat tctgtggaaa
    19921 tgataatagg taattcagtt taatcccgaa ttttgaaggc gatttggaat ctgtttttga
    19981 ctcgttaaaa aagaaggggc gttgtacccc ttttctgtaa aatacttact ttaatctata
    20041 aatttctttt ctatgtctaa tccttacaat cttagcttta ttcttttcaa gtataaatct
    20101 aacgcgataa ggccatactt caaatttccg ctgtcccttg tattcacccg taagaagctc
    20161 ac
//
LOCUS       DATDRL010000348         7371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3306143, whole genome shotgun sequence.
ACCESSION   DATDRL010000348 DATDRL010000000
VERSION     DATDRL010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3306143"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VI935_11705"
     CDS             <1..1057
                     /locus_tag="VI935_11705"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HLE26291.1"
                     /translation="KKPSSFAIPETEAQIHTELIREKSPTANVIGYIEGSDQSLKDQV
                     IIIGAHYDHIGLGDGVSRGDDKTNKNKVHNGADDNASGAAGLIELAEYFSNHGASLKR
                     SLLFIAFSAEELGLIGSSYYVKHPKIPLGNTLAMINMDMIGRLRDDKLTVFGSGSSPE
                     WKPLLESFNSNLGLTIQTKDSGFAPSDQSVFYANDIPVLHFFTGVHKDYHAPGDDWQK
                     INSEGEKKILLLISDLIGKLNETSTKIALSKAKEEERAPSQFNVYVGTIPDYSEEGEG
                     VTLMGVKEGSPAEKAGLKGRDTIVEFGGQSIRDIYDYVYALGEVDPEVPTEIVILREG
                     KRIKLSVIPESRKDKIN"
     gene            1098..1838
                     /locus_tag="VI935_11710"
     CDS             1098..1838
                     /locus_tag="VI935_11710"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HLE26292.1"
                     /translation="MSHARLRIFVIASVIIHILAFSIVAFVWLPDAIVTNAQLIGFGV
                     IGASKEHKAGVGNIFVASQKVAPKLENAVIKPITRKNREKEITNKGEAAIQPSKSMIE
                     PSTVNKSDYTNNEQTHLISNTSKASAESLKEGTQIGYEGLSVLGADGSGQAVEVGYPD
                     YKINPKPGYPIIARRNGYEGAVLLKVWVLEDGKVGKIEIEQSSGYEMLDKAALGAVKD
                     WIFMPGKRNGVPFPSWVTVPIKFQLRGG"
     gene            complement(1949..2632)
                     /locus_tag="VI935_11715"
     CDS             complement(1949..2632)
                     /locus_tag="VI935_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26293.1"
                     /translation="MRSKPFLTLFLTLGFALAFSVSSPSFAGGHSMMHGSFTKASDLR
                     LALNSLLREHVFLAAAATDAALGGRNTEFQAEAGALDANSVDLSKAIGSVYGPDAEKA
                     FLPLWRKHIGFFVDYTVGVATKDKANQEKAVSDLVQYTKDFGAFMHSASPALPVNVVA
                     DLVKTHVLTLKAVVDAQAAKNWAQADSDFFTECKNFTFTFVKPGTYPYFCKRHNPMRG
                     EIKVVPSTP"
     gene            complement(2731..4350)
                     /locus_tag="VI935_11720"
     CDS             complement(2731..4350)
                     /locus_tag="VI935_11720"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLE26294.1"
                     /translation="MKVCFINPAFPDSLWSFSGVASLVGKRGAFAPLGLATIAGCTPP
                     EFEIEIYDEEIEPINFETDADIIALTAFNVQARRAFKIAEEFRRRRKLVAMGGPYASL
                     CPEKCMPYTDVVLEGEGELIWQDFLADYKEGNYKRHYNQKEKISMLSSPPPRYDLLKI
                     KEYFEFPIQTTRGCPFTCEFCDIIITDGRVPRSKSVKQVLAEVLKLYELGVGSVVFTD
                     ANFIGSPKYTKELLEELKRFGKENNFPMSFACEATINLASWDEILSLMHAANFDRVFV
                     GVESPRKSSLVETKKLVNTRGSLLENIKKIQSYGMIVVAGMIVGFDSDDKEIFQEQFE
                     FLMEAGIPFTTVGTLVALENTPLYERVKKEGRLIESAYEDMRGHGASDTNFIPRQMTL
                     EELIKGYNWLIRALYTYDNYSKRILTMIDNFYRNGNEDRVGGKRPYLPGFLHTQNKPY
                     LSSELYKTIINVLSYYLITKDGKRRWFFISSLLKTLRGGANSRRLAGTLSLLVLHKHF
                     HDYVTKAHGDPEIISSISPYTPRRFRSSTSA"
     gene            4870..6189
                     /locus_tag="VI935_11725"
     CDS             4870..6189
                     /locus_tag="VI935_11725"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLE26295.1"
                     /translation="MPDYDLLISKIEHIVGREYLYYSHGGFLGFAIDGKVPGLLVYPA
                     NSDEVSAILRVANEEEASICPWSQGTKIVIGNIPKSLDIILFTSRLNKVIEHDQGNLT
                     VTVEPGVKLSELQSVIKDKKQFLPLDPMFSFRCSIGGVIASNSNGPRRLRYGSVRDLL
                     IGIKAVLPTGERIKAGGKVVKNVAGYDMCKLFVGSFGTLGIITEATFKLYPLPEVEKT
                     VLLSFENSAQAFEFISRLLESVVLPSSLEALNYSTLKVLSKKIDISFMGNTCCLVIRL
                     EGFEESVKRECDEIRKMGIDYARILEGPEQEGLWTALTDFPYLAGSNFRFKVSAPTSI
                     TFAIFNKVEGYSKNLGVIINLISHAGSGIVYGFMSTEEMDKLILFINELGSYVAEAGG
                     YFVVESASTEIKHKVNIWGHLPGGVEIMRKIKGSFDHKGLMSPGRLL"
     gene            6313..7002
                     /locus_tag="VI935_11730"
     CDS             6313..7002
                     /locus_tag="VI935_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLE26296.1"
                     /translation="MDNQVIAIVDDEEDIVELVSHHLKRDGFKVKEFYNGRDFLSFIR
                     SLLPDLLILDIMLPGADGLEVCRMLKNNSRTSSLPIIMLTAKGTEADIVVGLELGADD
                     YIVKPFSPRELVARVKTILRRSRKEDGADNLIRIGPLMVDSEKFEAAVEGKKVELTTT
                     EFKILEVLAEGRGKVFTRDQLLKKKRIWGDEKLIYDRTIDVHIKNLREKLGKCGIMVK
                     TIRGIGYKLEE"
     gene            7011..>7371
                     /locus_tag="VI935_11735"
     CDS             7011..>7371
                     /locus_tag="VI935_11735"
                     /inference="COORDINATES: protein motif:HMM:NF028046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HLE26297.1"
                     /translation="MKTFWKHFILNLFIISLIFFLFTILIIRELEKHDKALTEERLLT
                     EANLLREVLKSPITQGKKEEIKFLVSEIGKKIEIRITVIDKDGVVIGDSEEDPARMEN
                     HAKRPEVIDAISKGSGKS"
BASE COUNT         2211 a         1388 c         1648 g         2124 t
ORIGIN      
        1 aaagaaacct tcttcctttg ctattcctga aactgaagcc cagattcata ccgaacttat
       61 ccgtgaaaaa agccctactg caaacgttat aggttacatc gagggttctg atcaatcact
      121 caaagatcag gtgataatca ttggggccca ttatgaccac ataggtttgg gtgacggtgt
      181 atcaagagga gacgataaaa caaataagaa caaggttcat aacggtgcgg atgataacgc
      241 ttcaggtgct gcagggctta tagaactagc cgagtatttt tccaaccatg gtgcttctct
      301 taaaaggagt cttcttttta ttgccttttc ggctgaggaa ttggggttaa taggttcttc
      361 ttactatgtt aaacatccta aaataccttt gggtaataca ctagcgatga ttaatatgga
      421 tatgattggt agactaagag acgataaact aacagttttt ggatctggtt catctcctga
      481 gtggaaacct cttcttgaat cttttaattc taaccttggt ttaacgatac aaacgaagga
      541 ctccggtttt gccccaagtg accaaagcgt attttacgcc aatgatatcc ctgtacttca
      601 tttttttact ggagtgcaca aagactacca tgctcccggt gacgattggc aaaagataaa
      661 ctcagaagga gagaaaaaga ttcttttgct tatttcagat ttaatcggca agctgaatga
      721 aacatcgact aagatagcgt tgtctaaggc caaagaagaa gaaagagcac cctctcaatt
      781 taatgtctat gttggaacaa taccagatta ttcagaggaa ggagaggggg taacacttat
      841 gggtgtaaaa gaaggtagtc ctgctgaaaa agctgggctg aagggaagag acacaatagt
      901 agaattcggt ggacagtcta taagagacat ctatgattat gtttacgctt tgggggaggt
      961 ggatccagaa gtcccaaccg agattgttat attaagggaa ggcaaacgga tcaaattaag
     1021 tgttatacca gagtcaagaa aggataaaat taattagaat tattagaaat aaactctgtt
     1081 tagctgtgga tgtttttatg tctcatgctc gtcttagaat atttgttatt gcttcagtta
     1141 ttatacacat cttagctttc tctatcgtgg cttttgtgtg gctgccggat gccatcgtta
     1201 ccaacgcgca attaataggt tttggtgtaa ttggtgcctc caaagagcat aaggcaggag
     1261 taggtaacat ttttgtagca agtcaaaaag tggcacccaa actagagaat gccgttatca
     1321 aacctatcac aaggaaaaat cgagaaaagg aaattacaaa taagggagag gctgctattc
     1381 aaccctctaa atcaatgatt gaaccaagta cagttaataa aagtgattac acaaataatg
     1441 aacagacaca tctgatttca aatacttcaa aggctagcgc agagtcttta aaggaaggaa
     1501 cgcaaatagg atatgaagga ctatctgtgc ttggtgccga tggctcaggg caggctgttg
     1561 aggtgggtta cccagattat aaaataaacc ctaagcctgg gtatccaatc atcgcaagaa
     1621 gaaatggata cgaaggtgca gttcttctca aggtatgggt gcttgaggat ggtaaggtag
     1681 gtaaaattga aatcgaacag tcctcaggct acgaaatgct tgataaagct gcccttgggg
     1741 cggtgaagga ttggattttt atgcctggaa aaaggaacgg tgttccattc ccaagttggg
     1801 ttacagttcc gatcaagttt caacttaggg gtggatgaaa tcgtagcttt tggctattcc
     1861 ttataacatt accccagtca aaatcgtagt gttgcacaaa caagtctgtg cctacaattt
     1921 ttgagtactt acttataaag ccttgatctt aaggtgttga gggtacaact ttaatctctc
     1981 cgcgcatcgg gttgtgacgt ttacagaaat aggggtaagt gccaggcttt acaaaggtaa
     2041 acgtgaagtt tttacactct gtaaaaaaat cagaatctgc ctgcgcccaa ttcttagctg
     2101 cttgagcatc aaccactgcc tttagagtaa gaacatgggt cttaaccagg tcagccacaa
     2161 cgtttacggg aagggctggg ctagcagagt gcataaaggc accgaaatcc ttggtgtatt
     2221 gtactaaatc agacaccgct ttttcctgat ttgccttatc ctttgttgca acgcctactg
     2281 tgtaatcgac aaaaaacccg atatgtttgc gccacagtgg caaaaacgcc ttttcagcat
     2341 cgggaccgta aacagatcca attgctttgg aaaggtctac tgagttagca tcaagggcac
     2401 ccgcttctgc ctgaaactct gtattgcgtc ctcctaatgc cgcatctgtt gctgcagcgg
     2461 caaggaaaac atgctcacgg agcaaagagt taagcgctaa gcgcaggtca gaagccttgg
     2521 tgaaggagcc gtgcatcatt gaatgccctc cagcaaagct tgggctagat actgagaatg
     2581 ctaaggcaaa acccagcgtt agaaataagg ttaaaaatgg tttacttctc atctttttac
     2641 ttcctccgtg ttttggtttt tgcctaatat ataggattgg gtttatatgt ttggattaaa
     2701 tgtgtggtat taatctgcaa aaatgaaaag ttaggcacta gtactggaac gaaacctcct
     2761 aggggtatac ggagagatgg aacttattat ctctgggtct ccgtgagcct tagtgacgta
     2821 atcatggaaa tgcttgtgta atacgagaag tgataaagtc ccagccaacc tccgagagtt
     2881 tgctcctccc ctaagtgtct taagtaatga cgaaatgaaa aaccacctcc gttttccatc
     2941 ctttgtaatt aaataatacg aaagtacatt tataattgtt ttatatagtt ctgaagaaag
     3001 gtaaggcttg ttctgtgtat gcagaaaacc tgggaggtat ggccttttcc ctccaactct
     3061 gtcctcgttt ccattcctat aaaaattgtc tatcatggtt aaaatccgct tggaatagtt
     3121 gtcgtaagta tagagcgcac ggataagcca gttgtaaccc ttgattagtt cttcaagggt
     3181 catctgtcta ggtatgaagt ttgtgtcact tgcaccatgc ccacgcatat cttcgtaggc
     3241 ggattcaatt aaccgtccct ctttttttac tcgttcgtaa agcggggtat tctccagggc
     3301 aaccaaagtg cctactgtgg tgaagggaat gcccgcttcc attaagaact caaactgttc
     3361 ctggaatatt tctttatcat cgctatcgaa ccctacaatc attccggcaa caacaatcat
     3421 gccgtaggat tgaatctttt ttatgttttc taaaagtgat ccgcgtgtat tcacaagctt
     3481 ttttgtctct acaaggcttg acttccttgg ggattctaca cctacaaaaa cacggtcgaa
     3541 gttcgcagca tgcatcaaac tgagaatctc atcccacgac gcaaggttta ttgttgcctc
     3601 acaggcaaag ctcattggga agttattttc tttaccgaat cttttaagct cttcaagtaa
     3661 ctctttagta tatttagggc taccgatgaa attagcgtca gtgaacacga ctgaaccaac
     3721 tccaagttca tatagtttta gtacctccgc caagacttgc ttaactgatt tactccttgg
     3781 aacccttccg tctgtgatga taatatcgca gaactcgcag gtaaatgggc agccgcgcgt
     3841 tgtctgtata ggaaactcaa aatattcctt aatctttaga aggtcataac gtggcggagg
     3901 cgatgaaagc atagaaatct tctctttctg attgtagtgc cgtttataat tcccttcttt
     3961 atagtcagct aaaaagtcct gccaaattag ttctccctct ccttcgagca caacatctgt
     4021 gtacggcata catttttccg gacagagact tgcgtaaggg ccacccatag caactaactt
     4081 tctacgcctt ctaaattcct ctgctatttt aaaagccctt cttgcctgta cattaaatgc
     4141 agttagggca attatatcag catcagtctc gaagtttatg ggttcaatct cttcgtcata
     4201 aatttcgatt tcgaattcag gcggagtaca accagcaata gtcgcaagcc ccaaaggcgc
     4261 gaatgctcct cttttaccta cgagtgaggc aactccgcta aaactccata gtgaatctgg
     4321 aaaagctgga ttaataaaac aaaccttcat tcgatcaacc tcaggggttt aacttgataa
     4381 ttcccagtag cctgcttgct acagaatttc cactttgctg tcattgcgag cgttagcgaa
     4441 gcaatctcga attttaagat ttagaagcca gtttagaact gagattgctt cgtccccttc
     4501 cccccgactt gatcgggggt cgctcagggt tcctcgcaat gacagtcgtg acgatgttat
     4561 tataagaccg taccatgcgg cttgctgtag tgttcttcat ttcttaaagt atataacaaa
     4621 taaatcctct cttcactgaa aattttttca tgataagata atagaaattc gtgctgtaat
     4681 tttaatatat cgataggcag ggttttctaa ccccgtccgc aaatacggga caagagtgtc
     4741 ccgcctattc gtatgtgtaa ggcaagaatg tcgccaatac cggatagggg gttttttaac
     4801 ctactaaaat tcgcaacagg acctaccctg tgcatgtcga ggggataacg cctctactta
     4861 attttaacga tgccagacta cgatctttta atctcaaaaa tagaacacat cgtaggcaga
     4921 gaatatctct actactctca tggtggattc ttaggttttg cgatagatgg taaggtgcca
     4981 ggactccttg tttatcctgc caactcagat gaggtttcag caattttacg tgttgccaat
     5041 gaagaagagg cttcgatttg cccgtggtct caggggacaa aaatagtaat tgggaacata
     5101 cctaaaagct tagatattat cctctttaca tctcggctaa acaaagtcat tgaacacgac
     5161 caaggaaacc ttacagtcac tgttgagccc ggggtgaagc tttcagagtt gcagagtgtc
     5221 attaaggaca aaaaacagtt tcttcccctt gaccccatgt ttagctttag gtgttcgatt
     5281 ggtggggtta tagcatcaaa ttcaaacggg cctagaagat taagatatgg gtcggtaagg
     5341 gatttactga ttggaataaa ggcagtgctt cctactgggg aaagaatcaa ggctggagga
     5401 aaggtagtaa agaacgtggc aggctatgat atgtgcaaac tttttgttgg ttcttttggg
     5461 actctgggga taataactga agctactttt aaactttatc ctttaccaga ggtagagaag
     5521 acggttttgc tttcatttga aaattctgca caggcattcg aatttatatc gagattgctt
     5581 gagtcagtgg ttcttccctc ttctctagaa gcgttgaatt actccacgct taaggtttta
     5641 tcaaagaaga ttgacatatc atttatggga aatacatgct gtctggttat taggttagag
     5701 ggctttgagg aatcggttaa aagggagtgt gacgagattc gaaaaatggg tatagactac
     5761 gctcgaattc ttgagggtcc cgagcaagag ggattatgga ctgccctaac agattttcct
     5821 tatttggcag ggtcaaactt tagatttaag gtaagtgctc caacctcaat cacgttcgcc
     5881 atcttcaata aagtcgaggg gtattcgaaa aatcttgggg ttatcataaa ccttatttct
     5941 cacgctggga gcgggattgt ttacggattt atgtcaaccg aagaaatgga taagctaatt
     6001 ctgtttataa atgaattagg ttcttacgtt gcagaagccg gcggatactt tgtagtagaa
     6061 tcagcttcta ctgagattaa gcacaaggtt aatatatggg gtcatcttcc gggtggcgta
     6121 gagataatgc gtaagattaa aggaagtttt gatcataagg gcctaatgag tcctggaagg
     6181 ttgttataga agcggttgta ggagcgatac ccccattaga tacacgccat ggcgtgtatc
     6241 tacactaggt tggttctagt ttggtgaagt gagagattgt aaaattactc atcttagtaa
     6301 ataatatttt ttatggataa ccaggtaatt gcaatagtag atgatgaaga ggatattgtg
     6361 gaacttgtga gccatcactt aaaaagggat gggtttaagg taaaggagtt ctataatgga
     6421 agggattttc tttcatttat aagatccttg cttcctgatc ttttaattct cgacataatg
     6481 cttcctgggg cggatgggct tgaagtctgt aggatgctga aaaataacag ccgcacatca
     6541 tcccttccaa taatcatgct tactgcaaag ggcacagaag ccgatatagt tgttgggcta
     6601 gaactcggtg ctgacgacta tatagtaaag ccatttagcc cgcgcgagct tgttgccaga
     6661 gtaaagacca ttctaagaag atcgagaaaa gaagatggag cggacaatct tataaggatt
     6721 ggtcctctga tggttgattc agaaaaattt gaagctgcag ttgaaggaaa gaaagtagag
     6781 ctaacaacaa ctgagtttaa gattcttgag gtactggcag aaggaagggg aaaggtattc
     6841 acacgggatc agttgcttaa gaagaagagg atttggggtg atgaaaaatt aatctacgat
     6901 agaacgatag atgttcatat aaaaaatctc agagaaaaac taggtaaatg tggaatcatg
     6961 gtgaaaacaa tacggggcat aggctataag ttagaagagt agggaaaaat gtgaaaacat
     7021 tttggaagca tttcatactt aacctattca tcatttcttt aattttcttt ctatttacta
     7081 tccttataat ccgcgaactc gaaaaacatg acaaggcgct aacggaagaa aggttattaa
     7141 ccgaagcgaa cttgcttcgt gaggttctta aaagtccgat tactcaaggc aaaaaggaag
     7201 agataaaatt ccttgtttca gaaattggca aaaaaataga gataaggatt acagtaatag
     7261 ataaggatgg agttgtgatc ggtgattctg aagaggatcc tgctaggatg gaaaaccatg
     7321 caaagagacc agaagtaata gacgcaattt ctaaaggaag tggaaaaagt c
//
LOCUS       DATDRL010000349         3364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2255472, whole genome shotgun sequence.
ACCESSION   DATDRL010000349 DATDRL010000000
VERSION     DATDRL010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2255472"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..1493)
                     /locus_tag="VI935_11740"
     CDS             complement(312..1493)
                     /locus_tag="VI935_11740"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLE26298.1"
                     /translation="MELNIILKLGIILSVLFIAAYVVRRLSSVPSIVVYVILGLLLGA
                     FVDRRSEAVLDFFSEIGLVLLFFFLGLEFSIERIVSVARKIWAAGVLDLIINFCLGFF
                     VGYFLHFDITACIFLGAITYASSSAIVLKLLTDLKRVANIETETILGVLIFEDIVASI
                     LLALLSGIERESTKEASIIFTVLFTLVKVAFLLGASLLIARIFASNLRNLIDKIAEEE
                     FLAVFIIGSIFVLSGFVKFLGISEAFGAFLAGLIFAESNRLEPVEKIALPLKDIAIAI
                     FFFSFGVSIEITSDFHNLIVALTFLFFATFLGKILSGYIGARLSGLGNTPSLRAGYSM
                     VARGEFSVVLAYAAPRGLGIHALSGLYILLLAFIGIFSMIYAPNLARRTASVFRRKPS
                     A"
     gene            complement(1505..2008)
                     /locus_tag="VI935_11745"
     CDS             complement(1505..2008)
                     /locus_tag="VI935_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HLE26299.1"
                     /translation="MKLSEVDLPGIGKKFTLLTSKGIAISLVVHNTGKREIYLFPAGE
                     EEEASCILELSDEEARQLGTLLMGAFFAPVVVDKEKMDLLLKGLVIEWIELSTNSPAV
                     GKSIKELAIRRKTGASVIAILRKEKDIIPSPSPEEIFKADDTLIALGTREQISNLVEL
                     ITPKPSR"
     gene            complement(2083..3210)
                     /locus_tag="VI935_11750"
     CDS             complement(2083..3210)
                     /locus_tag="VI935_11750"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HLE26300.1"
                     /translation="MKEIKLGVSISLSGRYSVQGTESFDGLTLWVKDTNQSGGILIKK
                     KGKKIPVNLIYYDDGSSSETCKILIDKLIIKDKVDVLIGPYSSGLTLSAAPIVDKYKK
                     ILWNHGGSSDEIFEKGFRYIVSTTSPASKYLHGIIDMVRTIDKEAKKIALIQADDSGF
                     STNVAEGAKRYGKEKGFQVTNFKYQSGTKDFSFLLNNVKQNNPDIILSAGRAEDDLIL
                     AKQILEHKVVAKAIGLVVAGIKHFKEQLGKDAEGFLGPTQWERGIKIKPDFGPTPEEF
                     FKRFKETYGKEPDYPAAQGYNIGLVIQKGIEESGTLEDISIRETAGRTQFRTFYGDFK
                     IDPVTGNQIGHKMVIVQWQGSNKFIVFPKELSEKDPLYPKP"
BASE COUNT          994 a          703 c          612 g         1055 t
ORIGIN      
        1 cttgagcata tcgtgtagtt gtttcatcgg gctcatcgga gttagttata agaactacat
       61 taatcccaga gtttgcaagc tcaagtgcac aaatcgaacc tgcaatacca catccaatga
      121 ccacacaatc agtttttata attttcataa ttagctccga taaagggtta taagaaatat
      181 aaaacatctg ttacgtgaat aaaacattct gttttaatat cgccaaaatt tatagtcggt
      241 taactaactc acatttagcg tatagaaatg tggaatttag ctctacttag gtttcctttt
      301 atgctttttc cttaagcact tggctttctt ctaaatacac tagctgttct tcttgcaagg
      361 tttggagcgt agatcataga aaaaattcca ataaaggcta aaaggagtat gtaaagccca
      421 gaaagggcat ggatcccaag tcctctagga gcagcgtatg caagaactac ggagaactct
      481 ccccttgcca ccatagagta tccagccctg agagatggag tattcccaag accggaaagc
      541 cttgctccta tatatccgga taagattttt cctaagaacg ttgcaaaaaa gagaaatgta
      601 agagccacta tcaaattgtg aaagtcactt gtaatttcta ttgatacacc aaatgaaaaa
      661 aagaaaatgg caatagctat atcttttagt ggaagggcga tcttttctac aggctccagc
      721 ctgtttgatt cagcaaatat taatccagca agaaacgctc caaatgcctc ggatatacct
      781 aagaatttta caaagccaga taatacaaag atggagccga taataaaaac tgcaagaaac
      841 tcttcctcgg cgattttatc gatcagattt cttagatttg atgcaaaaat tctagctata
      901 agaagcgatg ctcctaaaag aaaggcaacc ttaacaaggg taaatagaac tgtaaagata
      961 attgaggctt ctttggtgct ctctcgttct attcctgaga gaagcgctaa aaggattgaa
     1021 gccactatat cctcgaatat cagcacaccc aaaatcgttt ccgtttctat gtttgcaacc
     1081 ctttttaaat ctgtaagaag tttcaaaact attgcagagc tacttgcata ggttatggct
     1141 ccgagaaaaa tacatgctgt tatatcgaag tgaagaaaat aacccacaaa gaatccaaga
     1201 cagaaattta taatcagatc aagaacccct gccgcccaga tctttcttgc aaccgatact
     1261 atgcgttcga ttgagaattc caagccgagg aagaagaaaa gaagtaccag tcctatctct
     1321 gagaaaaaat cgagaacggc ttcacttcgc ctgtcaacga acgctccgag taataaaccg
     1381 aggattacat aaacaacgat ggaagggaca ctacttaaac gtcttactac atacgctgct
     1441 ataaaaagca cggagaggat tattccaagt tttaaaatta tgtttagttc caaggttttg
     1501 tcttttatct actgggtttg ggggtaatta gctctactag attggatatt tgctctctcg
     1561 ttccaagggc aataagagta tcatcggctt taaatatctc ttcaggagag ggacttggta
     1621 ttatgtcctt ctcttttctt aaaatagcga taaccgaggc tcccgttttt ctccgaatag
     1681 ctaactcttt aatgctcttc ccaacagccg gtgaatttgt tgaaagttca atccattcaa
     1741 taactaggcc ttttaaaaga agatccattt tctctttatc tacaacaacg ggagcaaaaa
     1801 atgctcccat tagtagagtt cctagttgtc gtgcttcctc atctgataat tcaagtatgc
     1861 acgaagcctc ttcttcctct ccggcgggaa agaggtagat ttctcttttt ccggtgttat
     1921 gtacaacaag agaaatcgca attcccttgc tagtaaggag agtaaacttc ttccctattc
     1981 ctgggaggtc aacttcactc agtttcataa ttctattcct ccctcgattt taatagggca
     2041 aatcataaac agtgagaaga ttcatgtcaa aaaatttcgg atttaaggtt taggataaag
     2101 gggatctttt tctgacagtt ccttgggaaa cacaatgaat ttattactgc cctgccactg
     2161 aacaataacc atcttatgcc ctatctggtt gcctgtaacc ggatctatct taaaatctcc
     2221 gtagaatgtc ctaaactggg ttcttcctgc tgtttctcga atactaatat cttctagagt
     2281 tcctgattct tcgataccct tctgaataac tagtccaatg ttataccctt gggctgcagg
     2341 ataatcgggt tctttaccgt atgtctcctt aaatctctta aaaaattcct ccggagttgg
     2401 tccaaagtcg ggttttattt ttattccgcg ctcccactga gttggcccta aaaagccctc
     2461 ggcatctttt ccaagctgtt ccttaaaatg ttttattcct gctacgacaa gtccaatcgc
     2521 ctttgcaact actttgtgct ctaaaatttg cttagcaagt atgaggtcat cttctgcacg
     2581 gcctgcgcta agtataatat ctggattgtt ttgcttcaca ttgtttagta ggaatgaaaa
     2641 gtcctttgtg ccagactgat atttgaagtt agttacttga aaaccttttt ctttaccata
     2701 gcgtttggct ccttctgcaa cattagttga gaatccgctg tcatctgctt gaatgagagc
     2761 aatttttttt gcctctttat caatagttct caccatgtca ataatgccat gtaaatattt
     2821 gcttgcaggg cttgttgtac tcactatgta tctaaatccc ttctcaaaaa tctcatccga
     2881 tgaaccaccg tgattccaga gaattttttt atatttatct acaatcggtg ctgcggatag
     2941 ggtgagtccg cttgagtatg gacctattag aacatcaacc ttgtccttaa taataagttt
     3001 gtctattaga attttgcagg tttcagatga acttccatca tcgtagtaaa tcagatttac
     3061 cggtatcttt ttgccttttt tcttgatcag gatgccgcct gattgatttg tatctttgac
     3121 ccaaagggtg agaccatcga aactctccgt gccctgaact gagtaccttc cacttaatga
     3181 aatcgaaacg ccgagtttga tttctttcat ttatggtgcc agttgcatta aaaaagtact
     3241 caaagtttgt aggcacagac ttgtctgtgc catgcgcttt actttgcact aagcacgaac
     3301 aagttcgtgc ctacaatagt cgtttttcca ctggaaatag tgtaatacga cgcatttcat
     3361 tatg
//
LOCUS       DATDRL010000350         3374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_328020, whole genome shotgun sequence.
ACCESSION   DATDRL010000350 DATDRL010000000
VERSION     DATDRL010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328020"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VI935_11755"
     CDS             <1..255
                     /locus_tag="VI935_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HLE26301.1"
                     /translation="IVTVYADRSFSFIIKTPPVSYLLKKASGLDKGSSTASRVKVGKV
                     TRLQVREIANIKLPDLNSDDIEAAVKIVEGTARSMGIEVV"
     gene            357..1079
                     /gene="rplA"
                     /locus_tag="VI935_11760"
     CDS             357..1079
                     /gene="rplA"
                     /locus_tag="VI935_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HLE26302.1"
                     /translation="MARGKRYLEAKKKIDSAKLYEVREAIKIVEETKTAKFDETVEAA
                     VKLGIDPRQADQQVRGAIVLPHGTGRSVRVLVFAKGDKVKEAQETGADYVGAEDLIEK
                     ISKENWLDFDVTIATPDVMNLVGKLGKILGPRGLMPSPKVGTVTFEIGKAVKEAKAGR
                     IEIKNDKGGVIHTPVGKASFGSDKLRENLLVLMDTITRMKPSSSKGTFIKKVVLSTTM
                     GPGIKVDPLSIRDELKGFEIAA"
     gene            1189..1722
                     /gene="rplJ"
                     /locus_tag="VI935_11765"
     CDS             1189..1722
                     /gene="rplJ"
                     /locus_tag="VI935_11765"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HLE26303.1"
                     /translation="MLTKAVKDQVVKVFNERFKTSPSLFVVEYKGLTVKEVEGLRRRL
                     KKARADFKVAKNTLLKIASLETDIERIKDLFEGATAVAICKEDPVAVAKVFAESVKVL
                     PVLKLKGGIVEGKVIGVQDVSKLSQLPSRQALLTEFLGLLSSPISNFMGTLMELQRRL
                     LYAINSLKDLKKEKEEV"
     gene            1801..2199
                     /gene="rplL"
                     /locus_tag="VI935_11770"
     CDS             1801..2199
                     /gene="rplL"
                     /locus_tag="VI935_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HLE26304.1"
                     /translation="MADVTRADVISYLERASMLEISELIKEIEERFGVKAAAPQVAMV
                     STQVGGAGAAEAEAPEAEKTEFNVVLKSVGENKIQVIKVIREITSLGLKEAKELVEGA
                     PKTVKEGVKKEEAQAMKKKLEEAGATTEIN"
     gene            2299..>3374
                     /locus_tag="VI935_11775"
     CDS             2299..>3374
                     /locus_tag="VI935_11775"
                     /inference="COORDINATES: protein motif:HMM:NF016450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HLE26305.1"
                     /translation="MNFEGIETYKRKSFSKIPSVLEIPHLLDVQVKSYNEFLQADVSP
                     AQRREVGLHRALKTVFPIEDYNGRASLEYISYRLDKPKYETNECRMKGYTYVAPVYVI
                     FRLVIWDTQPGSGSERTIRDVKEQEVYFGEIPLMTQNGAFIINGTERVIVNQLHRSPG
                     VFFDQGKGKDQIVGKTSYSARIVPQDGRWLDFEFDSKDLMHVRIDRKKKFLITVLLKA
                     LGYTNKEIMLYFYPTETIYFETDGIKKSVDPEVLSYQKSSVDIVDPKTDEVIVKKGRK
                     FVRNLIDKLHQAKVERIPIQIIDIIGRPCADDVVDKKTGEVLLNFNEEIKEEKLQEIR
                     ARGIKEFKVFYIDNIAISASLRNT"
BASE COUNT         1150 a          541 c          843 g          840 t
ORIGIN      
        1 atagtgacag tttatgcaga tagatcattt tcgtttataa ttaaaactcc tccggtgtca
       61 tatttgttaa aaaaggcttc aggattggat aagggctcaa gtacagcatc aagggtaaag
      121 gtaggtaagg taacaaggtt acaggtaaga gagatagcta atataaagct gcctgatcta
      181 aattctgatg atatagaagc agcagttaag atagttgaag gaacagcaag gagtatgggc
      241 attgaagtcg tatgaagaat tcaggagtca gaaatcagaa tctagaattt agaagaaagg
      301 aagaaaactt ctggctgctg actcctgaat tctgaatata tttgaaggag gcaaaaatgg
      361 cgagagggaa aaggtactta gaggctaaaa aaaagattga ttctgcaaaa ctgtatgaag
      421 ttcgggaagc gataaagata gttgaagaaa caaaaactgc gaaatttgat gaaacagtag
      481 aggctgcggt caaactcggc attgacccac gtcaggctga ccagcaggtt agaggagcta
      541 tcgttcttcc acatgggaca ggaagaagtg tgagggtatt ggtttttgca aaaggtgaca
      601 aggtgaaaga ggctcaggag acaggtgcag attacgtcgg cgctgaagat ttaatcgaga
      661 agatttccaa agaaaattgg cttgattttg acgttactat tgcaaccccc gatgtaatga
      721 acctggtagg aaagcttggt aagatactcg gtcctagagg acttatgcca agcccgaagg
      781 ttgggactgt aaccttcgag ataggaaaag cggttaagga agcaaaagcg gggaggatcg
      841 agataaaaaa tgataagggc ggtgttattc ataccccggt tggaaaggcc tcttttggtt
      901 cagataagct tagagagaac ttacttgtgc ttatggatac aatcacaagg atgaagccat
      961 cttcgtcgaa agggacattt atcaaaaagg ttgtgctttc taccacgatg ggtccgggaa
     1021 taaaggttga tcccttaagc atccgagatg agctaaaggg atttgagata gcagcatgag
     1081 atacaagata caagatacag gatgcaggat gcaggataaa ggatacagat aaaaacctca
     1141 tgcatcgtgc accatgaatc atgcatcatt ttaataagga gtagaataat gttaactaag
     1201 gctgtcaaag atcaagttgt aaaagttttt aatgagagat ttaaaacaag tccttcacta
     1261 tttgtcgttg aatataaggg gctaactgta aaggaggtag aagggctaag gcggcgtctt
     1321 aaaaaggcta gggctgattt taaggttgct aagaacacgc ttcttaaaat agcatctctt
     1381 gagactgata tagaaaggat aaaagacctg ttcgagggag ccactgctgt agcaatatgt
     1441 aaagaggatc cggtagctgt tgctaaggtt ttcgctgaat ctgtaaaggt gttgcctgtt
     1501 cttaaattaa aaggtggcat cgtcgaaggg aaggtaatag gggttcaaga tgtctcaaaa
     1561 ctctctcagc ttccatcaag acaagcactt ttaacagagt tcctcggact actttcatct
     1621 cctatctcta attttatggg tacgttgatg gagctccaga ggcgcttact atacgcaata
     1681 aactcactca aagacctgaa aaaggaaaaa gaagaggtat aaatagttat agaaattttt
     1741 agaaaagagg tctatctaga acgacactaa atactaatca cataaaggag gttttagaca
     1801 atggctgatg ttacaagagc cgatgtaatt tcatatcttg agcgcgcaag tatgcttgag
     1861 atttcggagt tgataaagga gatcgaggaa agatttggcg taaaggctgc cgccccgcag
     1921 gtggctatgg tctctacgca agtagggggc gccggggcgg ctgaagctga agcgcctgaa
     1981 gctgaaaaga cggagttcaa cgtagtgcta aaatccgtcg gagagaacaa gatccaagtg
     2041 attaaggtca taagggagat tacctcactt ggtctcaagg aagcgaaaga gcttgttgaa
     2101 ggggctccaa agacagtgaa agaaggtgtt aagaaagaag aagcacaggc catgaaaaag
     2161 aagcttgaag aagcgggtgc cacgacagaa attaattagg actgtggttc gtgaatctag
     2221 aatctcgtat cgaattacga gccactatcc acgaattaca agccacgaaa aaataaacaa
     2281 aaagggggta gagataaggt gaatttcgag ggaatagaaa cttataaaag aaaaagtttt
     2341 tcaaagattc cgtctgttct tgaaataccg catcttcttg atgttcaagt aaaatcatac
     2401 aacgagtttc ttcaggcaga tgtaagtcct gcacaaagaa gggaagttgg acttcaccgt
     2461 gcattgaaga ctgtatttcc tatagaggat tataatggtc gcgcatcact tgaatatatt
     2521 agctaccgtc ttgataagcc aaagtacgaa actaatgagt gccggatgaa gggttataca
     2581 tacgtcgctc cggtctatgt aatttttcgt ttagtgatat gggatactca accaggctct
     2641 ggtagtgagc gaacgataag ggatgtaaag gagcaggagg tttacttcgg cgagattccg
     2701 ctaatgaccc aaaatggtgc attcattata aatggcaccg agagggttat agtcaatcag
     2761 ctacaccgct caccgggggt attcttcgat cagggtaaag gcaaggacca gattgtagga
     2821 aaaacctcat actcggccag gattgttcca caggatggaa ggtggcttga ttttgaattc
     2881 gattctaagg acctgatgca tgttagaatc gatagaaaaa aaaagtttct tatcaccgtt
     2941 cttctcaagg ccctcggcta taccaacaaa gagataatgc tttatttcta tccaactgaa
     3001 acaatctatt ttgaaacaga tggaataaaa aagagcgttg acccagaggt tctttcttat
     3061 caaaaatcta gtgtagacat agtggatcca aagactgatg aggttatagt aaaaaaggga
     3121 agaaagtttg taagaaacct aattgataag ctccatcagg caaaggtaga aagaattccc
     3181 attcaaataa ttgacataat aggtagacct tgtgcagatg acgttgtgga taagaagact
     3241 ggggaggtat tacttaattt caacgaggag ataaaagagg aaaaacttca agagattagg
     3301 gctcgaggga taaaggagtt taaggttttt tatatagata acatcgctat aagtgcatca
     3361 cttagaaaca ccat
//
LOCUS       DATDRL010000351        11434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1615209, whole genome shotgun sequence.
ACCESSION   DATDRL010000351 DATDRL010000000
VERSION     DATDRL010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11434
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615209"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VI935_11780"
     CDS             <1..862
                     /locus_tag="VI935_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002790570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26306.1"
                     /translation="TVNNRIQKFDALGHFPLTFGSFGLGDGQFNGPSGIEVDGTGNIF
                     VADFGNNRIQKFDSSGNFLLSFGTLGSGDGQFNGPSGVEVDSTGNIYVADTFNNRIQV
                     FGADSDFDGVPNSQDTDSDNDAIPNDTEDSGGGNALSTKHALDINGYGIAAAQQVSFL
                     DTDEDGIPNRLDLDSDGDGKSDIIEAGGRDADGDGMLDNFTDSDGDGLHDSVDPDQGG
                     KPLQLRDSDRDGVPNFLDPSDTSGSSGCSVASAGSTPSIPLYLLIPVFIAIKNAWQRC
                     RSGSKRQYRN"
     gene            complement(903..2213)
                     /locus_tag="VI935_11785"
     CDS             complement(903..2213)
                     /locus_tag="VI935_11785"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLE26307.1"
                     /translation="MSQDPTKRILILGGGFAGLDTALHLEKIFAKDKDVEITLVNNNN
                     YFVYTPMLAEVVASSIDAKHTVSSFREFFNKVTFKELEVRSIDLIKRIVNCYHCNVCE
                     IFNLEFDYLVVALGTVTSFHNTTGAEDNSFPLKNVYDAKVLRSHVIDMFEHADLEDDP
                     NKRRQLLTFVVVGGGYTGVEVAAELNDYIHTSRRFYRNVRPDEVKVIVVEHGPRIMRE
                     MSAGLADYAQGLLRERGMEIHLNKGVSSVLPNKVELDNGVTILTNTAIWAAGTSPHPV
                     IKGLPFEKDKGGRIIVNEYLEVPDFPSVWALGDCAHIPDPKTGEPYPPTGQHAVREAK
                     QLAYNIAATIREGDKKPFQYTTQGMLAPLGHRSAVAEIRGFKFSGFFAWFLWRTIYLI
                     KLPGWDRKIRVAIDWGLDLFFPRDIVQLRVPTRSRQQLEHQDTE"
     gene            complement(2656..3876)
                     /locus_tag="VI935_11790"
     CDS             complement(2656..3876)
                     /locus_tag="VI935_11790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HLE26308.1"
                     /translation="MLKRPLDELLEEYKGDYSIEYRRSSDDSFEDFYSSLDSQDEIGE
                     PSQLDVEENDNLKEFENKGEAQNKDFRLINAYFKEVSKEPLFSCAQELEIVTKMKRYE
                     RKSKEIKSKIENVLGKKLNNNNNSFIQDFRYLIEKCSSYTIRGLTRHRLNRLLKLYEA
                     CLKKTIHYRNCFIRGNLRLVATIAKKYVGRGVSFMDLLQEGNIGLITAVERFDHTRGY
                     RFSTYACWWIFQSIIRAIFSQSRTVKVPAYVLEKSGKVKQARSRLMEKLGREPSVEEI
                     SCEIDMSKKTLSRVLSCGERVIWLDSPIRQGERGTLMEVFKDQNSLPADSLISASLVP
                     ESVNQALKILDSRDREVIKMRFGIGYETSYTLEEIGERFNLTKERVRQIEKKALKKIK
                     KSRAAPALRSLIEI"
     gene            complement(3909..4553)
                     /locus_tag="VI935_11795"
     CDS             complement(3909..4553)
                     /locus_tag="VI935_11795"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HLE26309.1"
                     /translation="MTEITTKDRLLDTAERLFAEKGVKETSVRDITKAARTHLASVNY
                     HFRSKNGLIRGVIARRIKPLNKRRFELLDSYIAEAGDKPVTVERILRALIAPSIELYL
                     ENPHFLSFAGRMISTPDKQLHRIFASQMDEVFSRIKDQLMLAIPDIPETELMWRIHFT
                     TGAIIHTWTNNADLEIRSGGICKIKDKEAVINELIAFCGAGLRAGSQKSSTQIE"
     gene            5313..7334
                     /gene="tkt"
                     /locus_tag="VI935_11800"
     CDS             5313..7334
                     /gene="tkt"
                     /locus_tag="VI935_11800"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HLE26310.1"
                     /translation="MPGRDELDQLCVNTIRMLAVDCVQKAKSGHPGTPMGAADMVYVL
                     WDRFLKHNPSNPRWDDRDRFILSPGHASALLYSLLHLYGYDLSLNDLKNFRQWGSKCP
                     GHPEYGETPGVETTTGPLGQGFANAVGFAIAEAHLAASFNKPGYDIVNHYTYGICGDG
                     DLMEGITSEAASLTGHLGLGKLIFLYDDNQISIEGKTKDTAFSETVSKRFEAYRWHVQ
                     TVSNGHDRAAIEEALNKAREETCKPSLIICRTHIGYGSPEQDTAKVHGEPFNEEDYKA
                     TRRHFNFPEDKPFYIPVEALKHFRNALDRGNAWEEVWCTKFNAYAKEYPEPAERYKLI
                     MSGELPKDWDADVPAFTPEKDGVMATRDCSGKVINALAKQMKGYLIGGSADLAPSTKT
                     YMTGHGDVQFHDFAGDNLHFGVREHAMGAATNGMALHGGVIPYCATFLQFFNYMAQPV
                     RLAAMMKIRVIFVYTHDSIFLGEDGPTHQPIEHLAWLRSLPNIVTIRPADGNETAWAW
                     REAISRKDGPTALIFSRQKLPTLDQKKYASAEGVRKGAYIVSDCNGTPDIILISDGGE
                     LTYALGAQEKLSAEGINVRVVSFPSWELFESQPQGYKDSVLPPNVTRRLGIEAGGPFG
                     WDRYLGTVSDKNFIGMWGYGASAPFDVLEVKFGFTVENVVQKAKALLGR"
     gene            7690..10548
                     /locus_tag="VI935_11805"
     CDS             7690..10548
                     /locus_tag="VI935_11805"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HLE26311.1"
                     /translation="MANPLLEIQSYGQSIWYDYIRRGLIISGELQRLIDEDGLRGVTS
                     NPSIFEKAIAGSTDYNNALKSLEKQKDMEAKSLYEYLAINDVQDAADVMSSVYGKTKR
                     RDGYVSLEVSPYLANDTAGTLEEARRLWKAVGRDNILIKVPATKAGIPVIKQLISEGI
                     NVNVTLLFSQETYEQVAEAYISGLEAFVSKGGDPSGVASVASFFVSRIDTAIDNLGTA
                     KLKTAKSASEQAVLRSVIGKVAIANARLTYQQYKEIYRSVRWQALAAKGTQTQRLLWA
                     STSAKNPSYRDVLYVEELIGADTVNTVPAATFDAFRDHGRPRASLEEQLEEAHDTMEV
                     LGQAGISMQAVTDKLLDDGVRLFADAFDKLLSAVEKKRELLLGKKLNRQTYKLPENLD
                     AAVKTSLEDWRVNGKVRRLWSQDKTLWTNDDENRWLGWLGITEDQLAHIEHLKNIAEE
                     VKKERFSHALLLGMGGSSLCPEVMKMTYGRMDGYPELHVLDSTDPSQIKTFENKIDLA
                     NSIFIVSSKSGGTLEPNIFKQYFFERVKETVGADKAGSRFIAITDPSSKMQQVAEADG
                     FRHIFFGLPSIGGRYSALSNFGMAPSAIIGVDVERFLDLAEEMVHSCASCVPPEDNPG
                     VILGTIMGVLAKNGRDKVTIVTSPGIRDFGAWLEQLLAESTGKLGKGIIPVDQERLGP
                     PGVYGNDRLFAYLYLENSPDSTQDAGVEALQKAGHPVVRISLSDIYELGQEMFRWEIA
                     TAVAGAIIGINAFNQPDVEASKVATRKLTSEYEKTGSLPKETPILEDEGFKLFTDEKN
                     ADVLSKAAGSDKTITSYLKAHLDRIKAGDYFCISAYIEMNDAHQEHLQAMRHAARDAK
                     KVATCLGYGPRFLHSTGQAYKGGPNTGVFLQITCDDAIELQVPGQKYTFGVVKAAQAR
                     GDFQVLAERDRRVLRVHLGRDVQADLARLRQAVEAVVS"
     gene            10634..>11434
                     /gene="gnd"
                     /locus_tag="VI935_11810"
     CDS             10634..>11434
                     /gene="gnd"
                     /locus_tag="VI935_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HLE26312.1"
                     /translation="MQLGMIGLGRMGANMVRRLMRGGHKCVIYDRDADTVNAISKEGA
                     IGASSLQDFVSKLTPPRAAWLMVPAAVVDKMLNDLAPIMESGDIIIDGGNSYYVDDIR
                     RADELKSKGIHYVDIGTSGGVWGLERGYCQMIGGETEIVKHLDPIFKTLAPGMGNIPR
                     TPGRGKVSGTAEDGYLHCGPSGAGHFVKMVHNGIEYGIMAAYAEGLNILHQANVGKRG
                     RAVDAETAPLRNPEHYQYDFNLADIAEIWRRGSVVASWLLDLTAIALLE"
BASE COUNT         3251 a         2529 c         2553 g         3101 t
ORIGIN      
        1 cacagttaat aatcgcatcc agaagtttga tgcacttggc catttccccc tcactttcgg
       61 ctcatttggc ttaggcgatg gtcagtttaa tggtccatct ggcatagaag tggatggcac
      121 aggaaacatt ttcgttgcag attttggtaa taatcgcatc cagaagtttg attcaagtgg
      181 taattttctc ctctctttcg gcactcttgg ctcaggcgat ggacagttta atggtccatc
      241 tggcgtagaa gtggatagca caggaaacat ctacgttgct gacacattca ataatcgcat
      301 ccaggttttt ggagcagact ctgacttcga cggtgtgcca aattcccagg acactgattc
      361 ggacaacgac gccattccta atgatactga agacagtggg ggtggaaatg ctctttccac
      421 aaaacacgct ttagacatta atgggtatgg gatcgcggct gctcagcaag tcagtttttt
      481 agacactgat gaagatggaa ttccaaaccg cctggaccta gactcagatg gtgacgggaa
      541 atcggatatt attgaagcgg gcggtagaga tgccgatggg gatggtatgc tagacaattt
      601 caccgattct gatggtgatg gcttacacga cagcgttgac ccggaccaag gcggcaagcc
      661 acttcaattg cgtgatagcg atagagacgg agtacctaac ttcctagacc cctctgacac
      721 tagcggtagt agtggttgtt ctgttgcatc tgccggctct acaccatcaa taccattata
      781 tttacttatc ccggtcttta ttgcaataaa gaatgcttgg caaagatgta gaagtggtag
      841 taaaagacaa tataggaatt aactctaacc cgaggtatct agggtttctt attaggcttg
      901 tatcactcag tatcttgatg ttcaagctgc tgccttgatc ttgtgggaac cctgagctgg
      961 acaatatccc ttggaaagaa cagatcaaga ccccaatcta ttgccacacg tatttttcta
     1021 tcccaccccg gaagtttaat aagataaatt gttctccaaa gaaaccaggc aaaaaatcca
     1081 gagaatttaa atccccttat ttctgcaaca gcagatctat gtccgagcgg tgcaagcatt
     1141 ccttgtgtag tgtattgaaa tggcttttta tctccttccc ttatagttgc agcaatatta
     1201 taagccaatt gttttgcttc acgcaccgca tgctgaccag taggtgggta tggctcccca
     1261 gtttttgggt caggtatatg agcgcagtca ccgagagccc aaacactagg gaagtcaggt
     1321 acctctaagt attcatttac aataattctt ccacccttat ccttttcaaa gggaaggcct
     1381 ttgatcactg gatgaggtga tgtccctgcc gcccaaattg ctgtatttgt gagaatggtg
     1441 actccattat caagttcaac cttatttggc aagacactag aaactccttt attcaaatgt
     1501 atttccatgc cacgctcacg gagaagaccc tgtgcgtagt ctgctaaacc cgcgctcatc
     1561 tcacgcatga tgcgcggtcc atgttccaca acgatcacct taacctcatc cggtcgaaca
     1621 ttcctgtaga agcggcgact tgtatggata taatcattaa gctcagccgc aacctcaaca
     1681 cctgtataac ccccgccaac cactacaaat gtcaaaagct gtcgcctttt atttgggtca
     1741 tcttccaggt cggcatgctc aaacatatca ataacatggc ttctaagaac cttagcatcg
     1801 taaacattct tgagtggaaa agaattatcc tcggctcccg ttgtgttatg aaagcttgtc
     1861 actgtgccta gagcgacaac aagataatca aactcaagat tgaaaatctc acacacgtta
     1921 cagtgataac agtttacaat ccgttttatt aggtctattg aacggacctc aagctcttta
     1981 aatgtaacct tgttaaagaa ctccctaaat gaactaaccg tatgctttgc gtcaatacta
     2041 ctggctacta cctcggcaag cattggagta tagacgaagt aattattgtt gtttacaagg
     2101 gttatctcta catctttatc tttagcgaaa atcttttcca gatgaagcgc tgtatcaaga
     2161 cctgcaaagc cacctccaag aattagaatt cgtttagttg gatcttgaga catttactcc
     2221 tccatctttg tagcttaagt aattactata ctaacatgag tcaaatataa caagccatcc
     2281 cccaccctaa ccctctccga tcggtcaatc agattgtcat gcccgaatgt gtaatcgggc
     2341 atccatgcca ttctccttta tccgaggttg gaaaacctcg gctatcgttg tattgtctgg
     2401 tagaatataa acattcgata gaagcgcttt tcagtcgcgc ttgataagag tggcaattta
     2461 tcgcgccagc ctatcctgag ccaggtcccc gatttaatcg gggaagggaa ggtgctccaa
     2521 caagataatt atgcatatac cataaacttc ccgcaggaga ggcttcaagc ctcaacaaaa
     2581 tcggtgttct gtaattatca cggcaactag aaatatgcat ggctgacctc attccttaac
     2641 aatttgaaca aactattata tttcaatcaa gctcctaaga gcaggtgcag cacgcgattt
     2701 ctttatcttt ttcaaggctt tcttttcaat ctgtctaact ctttcctttg ttaaattaaa
     2761 tcgttctcct atctcttcca atgtataact ggtttcatat cctattccaa atctcatctt
     2821 gataacctcc ctatccctgc tgtcaagaat ttttaatgcc tgattaacgc tctccgggac
     2881 taatgacgcc gatataagtg aatcagccgg aagggaattt tgatccttaa agacttccat
     2941 aagggttcct ctttctcctt gcctgatagg ggagtctagc caaatcaccc tttctccaca
     3001 cgaaagcaca cggctaagtg tttttttcga catatcaatt tcgcaggaga tttcctcaac
     3061 actaggctcc ctacccaact tctccataag cctggaccga gcttgtttaa cctttccaga
     3121 tttctctaaa acatatgccg gaaccttgac tgttcttgac tgagaaaaaa tcgccctaat
     3181 tattgattga aatatccacc aacaagcata tgtggaaaac ctataacccc ttgtatgatc
     3241 aaacctctct acagccgtaa taagccctat gtttccttcc tgaagcaaat ccatgaagga
     3301 aacccctcgc cccacgtatt ttttagcgat tgttgcaacc agccttaagt ttcctcgtat
     3361 aaaacaattc ctatagtgga tcgtcttctt taaacaggcc tcataaagtt taagtagcct
     3421 gttgagtcta tgacgcgtta accctctgat agtataactt gaacattttt caattaggta
     3481 tcggaaatct tgaataaatg agttattatt gttattaagc tttttgccta atacattttc
     3541 tatcttggac tttatctcct tcgacttcct ttcatacctt ttcatcttgg tcacaatctc
     3601 caactcttgt gcacatgaga agagaggctc tttactgacc tctttgaaat aggcatttat
     3661 aagcctaaaa tccttgtttt gtgcctcgcc tttattctca aattccttga gattatcatt
     3721 ctcttctaca tctagttggg acggttcacc aatctcgtct tgagaatcaa gtgaagaata
     3781 aaaatcttcg aacgaatcgt cactcgatct acgatattct atgctataat ctcctttata
     3841 ttcctctaat aactcatcta atggtctttt caacattgca gttactcctt tgtgatattc
     3901 tcatagcctt attcaatctg agttgagctc ttttggcttc ctgctctaag acccgcgcca
     3961 caaaacgcta tgagttcatt gataaccgcc tccttatctt tgatcttgca aatacctcca
     4021 gaccggatct caagatcagc attgttcgtc caggtgtgaa taatagctcc ggtagtaaag
     4081 tgaattcgcc acatcagctc cgtttctggt atatccggaa ttgctagcat cagttgatcc
     4141 tttattcggg aaaaaacctc atccatttgt gaagcaaata tcctatgaag ttgtttgtcg
     4201 ggggtagaaa tcatgcgccc tgcaaagcta aggaaatgtg gattctcaag gtatagctca
     4261 atacttggtg caataagtgc acgtaaaatt cgctcaactg tgacgggttt atcacctgcc
     4321 tcagcaatat aggaatccag cagttcgaaa cgccttttat ttaagggctt tattctccta
     4381 gcgataacac cacgaattag accgttttta gaacggaagt gataattcac cgacgccaag
     4441 tgagtccgtg ctgcctttgt aatatctctt acagaggttt ccttcacccc cttctcagca
     4501 aaaagacgct ctgccgtatc aaggagacga tcttttgtag taatttcagt cattttaccc
     4561 tccattattt aaataataca tacgttcgta tcatacacat gttcgaattt taatttaatc
     4621 acctttttag aaatgtcaag accagtttcc taattagatt tctacttaga atttttgctt
     4681 gacaagtatt tgtttagccg caaagggtta cgactatctg aaccttaagt ggtttgattt
     4741 tttaagggaa cggcttctcg gtgatatcta cttttaaaca tatgtgattt agaaaaatat
     4801 agatttcaga ttaagcgtat taagcgtgac cctctagtcc cgaaatagta tggttgattg
     4861 atgagtgttt tatattcttt caataaaaaa caagatgcta atttaatcta aaaaatactg
     4921 acagcagagg atttttatcc gtttttgtag tttttgtcag ttaacaatgt aaagggagga
     4981 tttctcgctt cactcaatat gacaggttgt ggcttgaaac aatgaacccc tatttgttat
     5041 cccgaactaa tgcgagagat ctaaaagatt cctcacttcg ttcaaaacaa ggatttctcg
     5101 tggagcctgc actgagcacg aggaagtgct caaaatgaca ttattactgt agggctatgc
     5161 cattagtgtt tttgaattca tgaatgccaa ctattgcttc actaatgagc gcctttgctt
     5221 gacaaatatt tatctgttgt gttataaggg atatgagtct tttcttgcca atcgttattt
     5281 aacttaatta ggaacatcat ggaggaaaat taatgcctgg cagagatgaa ctagatcagt
     5341 tgtgcgttaa caccattcga atgcttgccg ttgactgtgt gcagaaagca aaatcaggac
     5401 accccggtac gcctatgggt gctgcagaca tggtctacgt gctttgggat aggtttttga
     5461 aacacaatcc ttcaaatccc aggtgggacg atcgcgaccg ctttatactc tcccctggac
     5521 acgcctctgc ccttctttac agcctcctac atctttacgg gtacgatctt tcgttaaatg
     5581 atctaaaaaa cttccgccaa tggggaagca aatgtcccgg tcatcctgaa tatggagaga
     5641 caccaggtgt tgaaacaaca acaggaccct tgggacaggg attcgcaaat gcagttggct
     5701 ttgctattgc agaggcacac cttgccgcta gcttcaacaa accaggctat gacattgtta
     5761 accactatac ctacggaatc tgtggcgatg gggacctgat ggagggtata acttccgaag
     5821 ctgcaagctt aacggggcat ctcggcttgg gcaagctgat ttttctttac gatgacaacc
     5881 agatctccat cgaaggcaag acaaaggata ctgcattttc cgaaaccgtt agcaagcgct
     5941 ttgaggcata tcgctggcat gtgcaaacgg tgtcgaacgg gcacgataga gctgctatcg
     6001 aagaggcttt aaataaagca cgcgaagaaa cttgcaagcc atcactcata atctgccgca
     6061 cgcacattgg ttatggaagc cctgagcagg atacggcgaa ggttcatgga gaaccattta
     6121 atgaggagga ttacaaagct acaaggaggc acttcaattt ccctgaagac aaacccttct
     6181 acatacctgt ggaggcactc aagcacttcc ggaatgcctt ggatcgaggg aatgcatggg
     6241 aagaggtatg gtgtacgaag ttcaatgctt atgcaaaaga atatccagaa cctgctgagc
     6301 gatataaact cattatgagt ggcgaattgc ctaaagactg ggatgcggat gtacccgcat
     6361 ttactcctga aaaagacggt gtaatggcaa ccagagattg ttcaggaaaa gtcatcaatg
     6421 ctctcgcaaa gcaaatgaaa ggctatctca tcggtggttc agcagacctc gcaccgtcaa
     6481 caaagactta tatgactgga cacggcgacg ttcaattcca cgactttgca ggggataacc
     6541 ttcactttgg cgtacgggaa cacgcaatgg gagctgcaac aaatgggatg gcgctacatg
     6601 gaggtgttat tccctattgc gctacatttc ttcagttctt caactacatg gctcaacctg
     6661 tacgacttgc cgctatgatg aaaatacgag ttatcttcgt atatacgcat gatagcatat
     6721 tcttaggaga agatggacca acacatcagc caattgaaca cctagcatgg cttcgctctt
     6781 tacccaacat tgtcaccata cgtcccgccg acggtaatga aaccgcatgg gcatggcgtg
     6841 aagcgataag tcggaaggac ggtccaaccg cgctcatttt ttcgcgtcag aaactcccaa
     6901 cacttgacca gaagaaatac gcatctgcag agggggtgag aaagggcgcc tatattgtct
     6961 cagactgtaa tggaacaccg gacatcatac tcatttcgga tggaggagag ctaacatatg
     7021 cgttaggtgc gcaagagaag ttatcagcgg agggaatcaa cgtgcgtgtt gtaagttttc
     7081 ctagttggga actctttgaa tcgcagcctc agggatataa ggattctgtt ttaccaccaa
     7141 acgttacgag acgattaggg atagaagcag gaggtccttt tggctgggac agatacctag
     7201 gcacagtcag tgataaaaac ttcattggca tgtggggata cggcgcatcc gcacccttcg
     7261 atgtcttaga agtgaagttc ggttttacag ttgaaaatgt agtacaaaag gcaaaggcac
     7321 tgctagggag gtgattaatg aaaaaccttg aagtaagttg cagagtatcg gcatattaac
     7381 agtcattccc ttcggtcatt gcgaaaaatc cttcagcttt gctcaggatt aactccgtga
     7441 cgaagcaatc tgatacgaag tgtcattccg aacctgtcct gagcctagtc gaaggagcag
     7501 tgaggaatcc caaacagatt gcttcgctag gcttgcgatg acaatggaaa ccctgtagca
     7561 agatgcaaga taaaaagtag gacgcaggaa acaagattca tcatgaatca tgcatcgtgt
     7621 atccttatga ttagtgtaaa ttcatgttga ttagtggcta aataaccatc gaagagaggt
     7681 gagtaacaaa tggcgaaccc actgcttgaa atacaaagtt atggacaaag catctggtat
     7741 gactacatcc gacgtggatt aattatatca ggcgaattac agaggttgat tgacgaagat
     7801 ggcttacgag gagtaacttc taacccttct atctttgaga aagccattgc aggaagtact
     7861 gactacaaca atgccctcaa atcactagaa aagcagaaag acatggaagc aaagtcgctc
     7921 tatgaatatt tagccataaa cgacgtccaa gatgccgctg atgtcatgtc gtccgtctat
     7981 gggaaaacaa agagacgcga cgggtatgtc agtttagagg tatcaccata tctcgccaac
     8041 gatacggcag gcacacttga ggaggcgcga agactctgga aagcagtggg acgtgataat
     8101 attttgatta aggtcccagc aactaaagca ggtatccctg ttattaaaca actcattagc
     8161 gaaggaataa acgttaacgt tacacttctc ttctcgcagg agacttacga acaggttgct
     8221 gaagcttaca tctctggttt agaagccttt gtgtcgaaag gcggtgaccc aagcggtgtc
     8281 gcaagcgttg caagtttttt cgttagcaga attgatacag ctattgataa cttaggaaca
     8341 gctaagttaa agacagcaaa aagcgcaagc gagcaagcag tgcttaggag cgtcataggt
     8401 aaggtagcta ttgccaatgc aaggttgacg tatcaacaat acaaggaaat ttatcgtagc
     8461 gtacgctggc aagcgctagc cgctaaagga acacaaaccc agcgactcct ttgggctagc
     8521 accagcgcta aaaatcctag ttaccgagac gttctttatg tcgaagaact catcggcgca
     8581 gatactgtaa acaccgttcc ggcagccacg tttgatgcct tccgcgacca cggacgtcca
     8641 cgagcgagcc ttgaagagca acttgaagag gcgcacgaca ctatggaagt gctaggacag
     8701 gctggtatct ctatgcaggc agtaacagac aaactgttag atgatggtgt tagactcttt
     8761 gccgatgcat tcgataaact tttaagcgcc gttgagaaaa agcgcgaatt gctccttgga
     8821 aagaaactca acagacagac atacaaatta cctgaaaatc ttgatgccgc agttaagaca
     8881 tctcttgaag attggcgagt gaatgggaag gtccgtcgtc tatggtctca ggacaaaaca
     8941 ctctggacta acgacgacga gaaccgctgg cttggttggt taggtataac agaggaccaa
     9001 ttggcacata tcgagcacct gaaaaatatt gcagaagagg tgaaaaaaga gcgcttctca
     9061 cacgcccttc tccttggaat gggcgggtca agcctttgcc cagaggttat gaagatgact
     9121 tacggcagga tggatggtta tccggaactg cacgttctag attctactga cccttcacaa
     9181 ataaagacat tcgaaaataa gattgatcta gccaactcta tcttcattgt gtcgtcaaag
     9241 tcaggtggca ccttagaacc aaacatcttt aagcagtatt tcttcgagcg agtgaaagaa
     9301 acagttggcg ctgataaggc gggcagccga tttatcgcaa ttactgatcc tagctccaaa
     9361 atgcagcaag tggcagaagc ggatggcttc cgtcatatct tctttggctt gcccagcata
     9421 ggcggaaggt attcagctct ctcaaacttt ggtatggcac cttcagcaat cataggtgta
     9481 gatgttgaga ggttccttga tctagcagaa gagatggttc atagctgcgc atcttgtgtg
     9541 ccgccagaag ataaccctgg tgttatacta ggaacgatta tgggcgtgct agcgaaaaat
     9601 gggcgtgata aggtcacaat cgtaacctct cctggtattc gggacttcgg cgcatggcta
     9661 gagcagcttc tggctgagtc gactggaaaa cttggaaagg gcataattcc tgttgatcaa
     9721 gaaagattag gaccgcctgg agtatatggc aatgaccgcc tttttgcata cttgtactta
     9781 gagaattcac ctgatagcac acaggatgcg ggagtagaag cccttcaaaa ggctggacac
     9841 ccagtagttc gaatctctct ctctgatatt tatgaactcg gtcaggagat gttccgatgg
     9901 gagattgcaa cggcggtggc aggtgcaatc atcggcatca atgcattcaa ccagccagat
     9961 gtggaggcga gcaaagtggc tacaagaaag ctgacttcag agtatgaaaa gacaggttct
    10021 ctacctaaag agaccccaat tcttgaagat gaggggttca agcttttcac cgatgagaag
    10081 aatgccgatg tcctttcaaa agccgcaggt agcgataaaa caattacaag ttatctaaaa
    10141 gcacacctcg acaggataaa agcaggagac tatttctgta tatccgcata cattgaaatg
    10201 aacgacgctc atcaagagca tctacaggca atgaggcacg ccgctcgtga tgcgaagaag
    10261 gttgctacat gtctaggcta tggtcctcgc tttttacact caactggaca ggcctataag
    10321 ggaggaccga atacaggcgt tttcctccaa atcacatgtg atgatgcgat tgaacttcaa
    10381 gttccaggac agaaatatac attcggtgta gttaaagcag cacaggctcg tggtgatttt
    10441 caggtgttgg ctgaacgtga tcgccgtgtc ttgcgtgttc atctgggcag agatgtacag
    10501 gcagacctgg caaggctacg tcaggctgtg gaagcggtgg tgagctgaag aacgtatggg
    10561 cggatggacg tgtgggtgac cgcccacact cccaaacgca cacacgccca aactcaaaca
    10621 ggaggtaata accatgcaac taggtatgat tggtttaggg cgcatggggg ctaatatggt
    10681 cagacggtta atgcggggtg ggcacaagtg tgttatatat gacagggatg cggatacggt
    10741 taatgctatc tctaaagagg gagcgattgg agcatcatcg cttcaagatt ttgtttctaa
    10801 actcacaccg ccgcgcgcag cctggctaat ggtgcctgcc gctgtggttg ataagatgct
    10861 aaacgacctt gcacccatta tggagagtgg tgacatcatc atagatggag gcaattccta
    10921 ctatgttgac gacatcagac gtgctgacga actaaaatct aagggaatac actatgttga
    10981 tattggaacg agcggcggtg tctggggtct tgagcgcggt tactgtcaaa tgatcggtgg
    11041 cgagactgaa attgtcaagc acttagatcc catctttaag acacttgcac cagggatggg
    11101 aaacatccct cgcacccccg gacgagggaa ggttagtggc acggcagaag atggttacct
    11161 gcattgtggt ccatctggcg ccggacactt tgtaaagatg gttcacaacg gtattgaata
    11221 tggtattatg gcggcatacg cagaagggct aaatatacta catcaagcca atgttggaaa
    11281 acgtggtcgt gccgttgatg ctgagactgc tcccttacga aatcctgaac attaccagta
    11341 tgacttcaat ctagcagaca tagcagagat atggagacgc ggtagtgttg tagcatcgtg
    11401 gcttcttgac cttacggcaa ttgcccttct tgag
//
LOCUS       DATDRL010000352         5528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_700084, whole genome shotgun sequence.
ACCESSION   DATDRL010000352 DATDRL010000000
VERSION     DATDRL010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700084"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VI935_11815"
     CDS             <1..349
                     /locus_tag="VI935_11815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HLE26313.1"
                     /translation="GRLSIKEISQNQHIPLNYLAKIMRKLVNKGIVQSMVGPEGGYVL
                     RKSPREITLREVYEAIEGDFRMIDCMEKDSICILHENCAQLPVWDKLQISMIKILEST
                     TLEDMLKQKREVM"
     gene            405..1835
                     /gene="sufB"
                     /locus_tag="VI935_11820"
     CDS             405..1835
                     /gene="sufB"
                     /locus_tag="VI935_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HLE26314.1"
                     /translation="MSVNFDDLTNQEYKYGFVTNIEQDIVPRGLNEDIIRLISSKKNE
                     PEWMLEWRLKAYRHWGTMKEPKWQNIHYAPIDYQSISYYAAPKKKPESLDEVDPELLK
                     TYEKLGIPLNEQKMLAGVAVDAVFDSVSLITTFKEKLAELGIIFCSFSEAVLEHPELI
                     KKYLGLVVPYNDNFFAALNSAVFTDGSFVYVPKGVRCPMELSTYFRINAENTGQFERT
                     LIIADEGSYVSYLEGCTAPKRDENQLHAAVVELITHDNATIKYSTIQNWYPGDKDGRG
                     GIYNFVTKRGRCLGRNSHISWTQVETGSAITWKYPSCILEGDNSVGEFYSVAITNNRQ
                     QADTGTKMIHVGKNTRSNIVSKGISAGFGQNTYRGLVQIHKSADGARNYTQCDSMLIG
                     DKCGAHTFPYIEVKNSSAQMEHEASTSKIGEDQLFYLKQRGLSAEDAVSMIVNGFCKK
                     VLSELPFEFAVEAERLLSISLEGSVG"
     gene            1891..2661
                     /gene="sufC"
                     /locus_tag="VI935_11825"
     CDS             1891..2661
                     /gene="sufC"
                     /locus_tag="VI935_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HLE26315.1"
                     /translation="MLEIRNLHVKVNEQEILKGVDLKVYAGEVHSIMGPNGSGKSTFA
                     QVLAGRDTYKVTEGEIIYQGKNLLEIKPEERACMGIFMAFQYPVEILGVTNTYLLRTA
                     LNAQRKFRGLEPVKPVEFAKLLKEKAKIVQMSDDLLQRSVNEGFSGGEKKRNEVFQMA
                     VLEPTLAILDETDSGLDIDALRIVANGVNAMRGKERAFIVITHYQRVLNYIITDYVHV
                     LVDGRIVKSGGKELALELESKGYTWVEEEDRTAMPANA"
     gene            2676..4022
                     /gene="sufD"
                     /locus_tag="VI935_11830"
     CDS             2676..4022
                     /gene="sufD"
                     /locus_tag="VI935_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HLE26316.1"
                     /translation="MDTIVMQSKESYLEGFEHFEKDSLVNDPTWLRSIRRAAISRFSE
                     LGFPTIQDEDWRFTNVGPIVRTPFQLAQNGRVEIDSRELERFIFSGLECSRLVFLNGQ
                     YSPELSSLGSRLDVLKIDSLAHALTVERDLLEDHLGRYADFQRDAFLALNTAFMKDGA
                     FVCIPSGTVLEEPIYILFVSSATSSPTITHPRNLILLEDNSQATIIEDYISLADDVYF
                     SNSVTEVVVGQNSVLSHYMIERESKKALNVSTMRFQLSRSSNVTFHSVLLGGALVRSN
                     VHPVMVGEGAECMINGLFMPTGRQHMDNYMRVEHASPHCDSRQLYKGILDGRSHGVFH
                     GRIIVHKNAQKTDAKQTNKNLLLSDEAQIDTKPQLEIYADDVKCTHGATIGQMDQNAI
                     FYLRSRGIAEDASRVLLLLAFAGESLQRMKVEPIRRGLEEIVMQRFAQGKPLDDVL"
     gene            4138..4551
                     /locus_tag="VI935_11835"
     CDS             4138..4551
                     /locus_tag="VI935_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HLE26317.1"
                     /translation="MATKGALIMDYNQMEYLLDHYKNPRNYGSLQNPDITHEEGNPSC
                     GDVIRIDLKITGNKVQKVKFTGKGCAISQAAASILTDMIVDKDVDYIKEINKDDMLSA
                     LGITLSPVRYKCGLLALKVLRSGVHGIKNWPGEEE"
     gene            4644..4958
                     /locus_tag="VI935_11840"
     CDS             4644..4958
                     /locus_tag="VI935_11840"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HLE26318.1"
                     /translation="MPEFVMVTKTSDILSGEIKSFDVEGEVIAICHLNGKFYAIKDEC
                     SHMKYPLSDGSLDGEIIICAWHGAKFNVKTGKVLSMPAVVPIETYQLKIEGDNIYVLI
                     EL"
     gene            4980..>5528
                     /locus_tag="VI935_11845"
     CDS             4980..>5528
                     /locus_tag="VI935_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLE26319.1"
                     /translation="MEKHKVALEGLDPYRIRNDFPIFERKIRGKPLIYFDNAATTQKP
                     NLVIETEKRFYEETNANVHRAVHTLSYESTVLYEDSHKKVAKFIGAKNWREIIFTRNA
                     TESINLVAYGWGLHNLRKGDEVLITIMEHHSNIVPWQMLRDQKGITLKILDVDDEGRL
                     KLDELPRLISERTKLVSVIHASN"
BASE COUNT         1696 a         1053 c         1309 g         1470 t
ORIGIN      
        1 aggaaggtta agcattaaag agatttctca aaaccagcac atcccattaa attacctggc
       61 aaagattatg cgaaaacttg ttaataaggg gattgtacaa tcaatggtag gacctgaagg
      121 tggttatgta ctgagaaaat cccctaggga gattacattg agagaggtat acgaagccat
      181 agagggcgac tttaggatga ttgattgtat ggaaaaggat tcaatctgta ttctacatga
      241 gaattgtgcc cagctcccag tctgggataa gcttcagatt tctatgatta agatacttga
      301 gagtactact ttggaagata tgttaaaaca aaaaagagaa gtaatgtaat cttagacgta
      361 gatgagggaa aaaactcaaa taacaaagca agaaggaggg cattatgagc gttaattttg
      421 atgatttaac aaaccaagaa tacaagtacg ggtttgttac caatatcgaa caggatatag
      481 tgccacgcgg tctaaatgaa gacatcattc gtctaatctc gtcaaagaag aatgagcccg
      541 agtggatgct agaatggcgc ctcaaggctt ataggcactg gggtactatg aaagagccaa
      601 agtggcagaa tattcactat gcgcctattg attatcagag catcagctac tatgcagcgc
      661 cgaagaagaa gccagagagc ctggatgagg tagaccctga attactcaag acatatgaaa
      721 agcttgggat tccgctcaat gagcagaaga tgttagccgg agtagccgtg gatgctgtct
      781 ttgacagtgt ctccttaata acaacgttca aggagaagct tgctgaactt ggtattatat
      841 tctgttcttt ttccgaggcg gtacttgaac atcctgaact aataaagaaa tatctcggat
      901 tggttgtgcc ttacaatgat aacttctttg ctgcactgaa ctcagcggtt tttaccgatg
      961 gatcattcgt gtacgtccct aaaggtgttc gctgtcctat ggagctatct acttacttcc
     1021 gaatcaatgc agaaaatacc ggccagtttg aacgcacact aataattgct gatgagggtt
     1081 cgtatgtcag ctatctggag gggtgcaccg ctcctaagcg agatgagaac cagcttcacg
     1141 ctgcagtggt tgagcttatc acgcatgata atgcaaccat caagtattcc acgatccaga
     1201 actggtaccc gggcgataag gacggtcgtg gtggaatata caactttgtc actaagcgtg
     1261 gcagatgttt gggacgaaat tctcacatat catggactca ggttgagact ggttctgcta
     1321 ttacctggaa gtacccaagc tgtatactgg agggtgataa ttctgttggt gaattctatt
     1381 ctgttgcaat aaccaacaat cgtcagcagg cggacactgg cacaaagatg atccacgtag
     1441 gtaaaaacac cagaagtaac attgtatcta agggtatctc tgctggcttt ggtcaaaaca
     1501 cctatcgtgg attggttcaa atacataaga gcgccgatgg agcacgtaat tacacccagt
     1561 gtgattctat gcttataggt gataagtgtg gcgcccatac atttccctat atcgaggtaa
     1621 aaaactcctc tgcccaaatg gaacacgagg catccacctc taagattggg gaggaccagc
     1681 tcttttacct taaacagcga ggtctatcag ccgaggatgc ggtatcaatg attgttaacg
     1741 gtttctgtaa gaaggttctc agcgaactgc cttttgagtt tgctgtcgag gcagagagat
     1801 tattaagcat tagcctcgag ggtagcgttg gataaatgta ataatgataa ttcgtaaaag
     1861 gagaagatat aaaagatgca caaaatgcct atgttagaaa ttagaaatct acatgtgaag
     1921 gtaaatgaac aagaaatcct aaagggggtt gacttaaagg tctacgctgg cgaggtccat
     1981 tcaattatgg gcccaaatgg atcaggcaaa agcacttttg ctcaagtcct ggctgggcga
     2041 gatacctata aggttaccga gggagagata atctaccagg ggaagaatct actcgagata
     2101 aagccagaag agcgtgcctg tatgggaatc tttatggctt tccaatatcc ggttgagatt
     2161 cttggtgtca ctaatacata cttgctccga accgctctca atgctcagcg aaagttccga
     2221 ggtcttgaac ccgtcaagcc tgtagagttt gccaaactac tcaaggaaaa agcgaagatt
     2281 gttcagatga gtgacgatct tctacaacgc tcagttaacg aggggttttc aggtggagag
     2341 aagaagcgaa atgaggtctt tcagatggcg gtgcttgagc caactctagc catactagat
     2401 gaaacagatt ccggtctcga tattgatgct ctaagaatcg tcgctaatgg cgtcaatgct
     2461 atgcgaggca aggaacgcgc atttatagtg attacccact accaacgggt gcttaactat
     2521 atcattacag actatgtgca tgtattagtt gatggcagga ttgtaaaatc aggcggcaag
     2581 gaattggctt tagagcttga gagcaagggc tatacctggg ttgaagagga agaccgcact
     2641 gccatgccgg caaacgcttg aacagggagc ttagaatgga caccattgta atgcaaagta
     2701 aggaaagcta cttagaagga tttgaacatt ttgagaagga ttcactcgta aatgacccaa
     2761 catggctacg ctctattcga cgggcggcaa tttccagatt ctctgagtta gggtttccaa
     2821 caatccaaga tgaagattgg cggtttacaa atgtaggccc gattgtacgt acaccctttc
     2881 agctagcaca gaacgggcgt gtagaaattg attctcggga gcttgagcgg tttatatttt
     2941 ctggactaga atgcagccgt ctcgtatttc taaatgggca atattcacct gagctttctt
     3001 ccctaggttc aaggcttgac gttttaaaga ttgatagcct tgctcatgcc ctaacagtag
     3061 agagggattt acttgaggat catcttggtc ggtacgctga ctttcaaaga gacgcatttt
     3121 tggccttgaa tactgcattt atgaaggatg gagcgtttgt gtgcatacca agtggcaccg
     3181 tgcttgaaga accgatatat atattattcg tgtcttctgc aacctcttca ccaacaatca
     3241 ctcatccacg caacctcatc ttattagaag ataatagcca ggcaacaatc atagaggatt
     3301 acatatcttt ggcggacgat gtatatttct caaactcggt cacggaagta gttgtgggtc
     3361 agaatagcgt acttagccac tatatgattg agcgggaaag taagaaggca ctcaacgtct
     3421 cgaccatgcg atttcagcta tctcgcagca gcaacgttac attccattcc gtgctactcg
     3481 gcggtgcgct tgtacgaagc aatgttcatc ctgtaatggt aggtgagggc gctgagtgta
     3541 tgatcaatgg gctcttcatg ccaaccggca ggcagcatat ggacaactac atgagggtcg
     3601 aacacgcaag ccctcactgc gacagccgac agctctataa gggtatcctg gatggcaggt
     3661 cccacggcgt cttccacggt aggattatcg ttcacaagaa cgctcaaaag accgatgcca
     3721 agcagactaa caaaaatcta ttactctccg atgaggctca gattgatacc aagccacagc
     3781 ttgagatata tgccgacgat gtgaagtgca cccacggggc gacaatcggc cagatggatc
     3841 agaatgcgat tttttatcta cgttccagag gaatagcaga agatgcatca cgtgtgcttc
     3901 tcctactcgc ctttgctggt gaaagcctcc agcgcatgaa ggtagagccg attcgaaggg
     3961 gtcttgagga gattgtcatg caacgcttcg ctcagggaaa gccgttagat gatgtccttt
     4021 gagtatttat acaaatcgat tttgcaatga ttcactgatt tttgccaaat gtcatccatt
     4081 tctccaagaa cgagcgtttt acgaattaaa tatataagat ccagaaagaa aaggcaggtg
     4141 gcaacaaaag gggctctaat catggattat aatcagatgg aatatctcct tgaccactat
     4201 aaaaaccctc ggaattatgg aagtcttcaa aaccctgata taacacatga agaaggaaac
     4261 ccttcctgcg gagatgttat aaggattgat ttaaaaatta caggtaataa agttcaaaag
     4321 gtaaaattta caggaaaagg gtgtgctatt agtcaagctg cagcgtcaat tcttactgat
     4381 atgatcgttg acaaggatgt ggattatata aaggaaatca acaaggatga tatgctttct
     4441 gcattgggga tcactctgtc tccagtaaga tataagtgtg gtctattggc cttgaaggtt
     4501 ttaaggtcag gtgttcatgg tataaaaaat tggcctggcg aggaagaata ggtttcaaat
     4561 ttgtgaatcg tggttcgtgt attgtgattt gctgtttgta gttcgagtca cgagtcactc
     4621 taataaaatt gagttagaat agaatgcctg agtttgtaat ggtaaccaag acatctgata
     4681 ttctttctgg agagatcaaa tctttcgacg tcgaaggaga ggttatagca atttgccatt
     4741 taaatggcaa attctatgct ataaaagatg aatgctcgca catgaaatat ccactctcag
     4801 atggttcatt agatggagag ataattatct gcgcttggca tggcgcaaaa tttaacgtga
     4861 aaactggtaa ggtgctttcc atgcctgcgg ttgtacctat tgaaacctat cagttaaaga
     4921 ttgaaggaga taatatatac gttttgatag agctttagta aaacagtaat tgagatttca
     4981 tggaaaagca taaagtggct ctagaaggtt tagatcctta tcgaataaga aatgacttcc
     5041 ctatatttga acgtaaaatt aggggtaagc cactaatata ttttgacaat gccgcaacta
     5101 ctcaaaaacc aaaccttgta atagaaactg aaaaaaggtt ttacgaagag actaatgcca
     5161 atgttcatag ggcggttcat acactcagtt atgagtccac ggttctctat gaagattccc
     5221 acaaaaaggt tgcaaaattt attggagcta aaaactggcg cgagatcatt ttcacaagaa
     5281 atgcaactga gtcaataaac cttgtcgcat acggttgggg gcttcataat ctcaggaaag
     5341 gggatgaggt acttatcaca attatggagc atcactcgaa tatagtccca tggcagatgc
     5401 ttcgagacca aaagggtatc acccttaaaa ttttagatgt agatgatgag ggaagattaa
     5461 agctagatga gcttccaagg cttatttcag aaaggacaaa actagtcagc gttattcacg
     5521 catcaaat
//
LOCUS       DATDRL010000353         3842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3074860, whole genome shotgun sequence.
ACCESSION   DATDRL010000353 DATDRL010000000
VERSION     DATDRL010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3074860"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /gene="hslV"
                     /locus_tag="VI935_11850"
     CDS             <1..343
                     /gene="hslV"
                     /locus_tag="VI935_11850"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HLE26320.1"
                     /translation="KFEAKLEEYRGNLRRAAVELARDWRTDRILRRLEALLAIADREA
                     MFIISGSGDVLEPDDDVIAIGSGGPFAQAAAKALLRHSKLNVREIAEESLKIAAEICI
                     YTNDKIIVEVL"
     gene            348..1673
                     /gene="hslU"
                     /locus_tag="VI935_11855"
     CDS             348..1673
                     /gene="hslU"
                     /locus_tag="VI935_11855"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HLE26321.1"
                     /translation="MNREAFFTPREIVSELDKYIVGQNKAKKAVSIALRNRWRRQRVI
                     PELREEIAPKNILMIGPTGVGKTEIARRLAKLAQAPFLKVEASKFTEVGYVGRDVESM
                     IRDLTDIAVKMISDEEKQSVKTKAEDLAEERILDLLYPKNKTADVSSFTDTREKLRKL
                     LREGKLNERYVDVEVNLRNLPVQVFTPSGMEEMDVNIADMLGNLFPKRTKKRKVKVPE
                     AMDILVHEEAQKLIDMDKVVKQAIERVEQSGIIFVDEMDKIAGRETAAGPDVSREGVQ
                     RDLLPIIEGCTVNTKHGMVRTDHILFIAAGAFHVSKPSDLIPELQGRFPIRVELDPLT
                     KEDFIRILTEPQNALIRQYKELLKTEGIELIFKDDAIIEVAEIAAYVNESTENIGARR
                     LHTVLERILEDLSFNAPDISKERIVIDTKYVKEMIADIVKDRDLSRYIL"
     gene            complement(1738..2499)
                     /locus_tag="VI935_11860"
     CDS             complement(1738..2499)
                     /locus_tag="VI935_11860"
                     /inference="COORDINATES: protein motif:HMM:NF022633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HLE26322.1"
                     /translation="MKNNFVNIDNPDLYIYRVFNVNRLIEMFKTKKLVLVRPELCDDP
                     FENFLIKSSTPLPSGGIMSFRGVADRFYGQCWTLYQESDAMWRIYSPDKNGAKVKTTI
                     RRLNEAIYNPNNKFASLSYYIGKVMYMTEEEIRKMFADPDFVTPTVLDHSGLGPVLTL
                     LLKGEAFEHEKEVRLVYRETTGRFKKDQKIIEFDIDPNTLFNEIVFDSRMSETDYEMY
                     SNEIKRCSFTKPISQSTLYQAPNLKINIYPQSSVY"
     gene            2673..3551
                     /gene="argB"
                     /locus_tag="VI935_11865"
     CDS             2673..3551
                     /gene="argB"
                     /locus_tag="VI935_11865"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HLE26323.1"
                     /translation="MKDLIQKAQTLVEALPYIREFYGKTLVIKYGGSIGGSELHNFAR
                     DIVLMKYVGMHPVVVHGGGPQIGNLLGRLGIKSKFIAGLRVTDAETMEIVEMVLVGKV
                     NQEIITAINSLGGNAAGASGKDGKIIVAKKVDISNMIDDGGDIAEDADLGMVGEVERI
                     NPQLIKVLESAGFITVIAPIGFDEEGRSYNINADHVAGKIAGALQAEKLILLTDVPGI
                     LDEEKNLISSLTESTARELMKNGIITSGMIPKVRCAIEALHEGVNKAHIIDGRVEHAL
                     ILEIFTDAGVGTEILL"
     gene            3548..>3842
                     /locus_tag="VI935_11870"
     CDS             3548..>3842
                     /locus_tag="VI935_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLE26324.1"
                     /translation="MKTEEIIARFDRYVMNTYSRHPIVLVGGEGCWVRDIEGKKYLDF
                     ITGIAVTNLGHSHPAVVKAIEDQARILIHVSNLFHIQPQVELAEILVKNSFADK"
BASE COUNT         1263 a          643 c          889 g         1047 t
ORIGIN      
        1 caaattcgag gcaaagctcg aggaatatag gggtaatcta agaagggctg ccgtcgagct
       61 ggcaagagac tggcgaaccg atagaatctt aagaaggctt gaggccctgc tcgctatagc
      121 cgacagggaa gcgatgttta ttatttcggg gagcggggat gttcttgagc ccgacgatga
      181 cgtaatagcg atcggctcag gcggtccttt tgctcaggcc gcggcaaagg cgcttttaag
      241 gcactccaaa ttgaatgtgc gggagattgc ggaggaatct cttaagattg cagcagaaat
      301 ctgtatttac acgaatgata aaattatagt tgaggtttta taaattaatg aacagggaag
      361 cattttttac tccgagagaa atcgtttcag aattagataa atacattgtg ggtcagaata
      421 aggcaaaaaa agctgtatca attgctctta gaaatcgctg gaggcgccag cgtgtcattc
      481 ctgagcttag ggaagagatt gctcctaaaa atatcctgat gattggccca acaggtgttg
      541 gcaaaacaga gatagcaaga agattagcaa agcttgctca ggctcccttt ttaaaggtcg
      601 aggcttctaa gtttacagag gtgggatatg tgggaaggga tgttgaatca atgattagag
      661 accttactga tattgctgta aagatgatat ctgatgaaga aaagcagtca gtaaaaacta
      721 aagctgagga tttagcagag gagagaattc ttgatctttt ataccccaaa aataaaactg
      781 ccgatgtttc ctcatttact gatacgagag agaaactcag aaagctttta cgggaaggaa
      841 aacttaatga aaggtatgtg gatgttgagg ttaacctgag aaacctcccg gttcaggttt
      901 ttactccctc aggtatggaa gagatggatg tgaatattgc agatatgttg ggaaatctct
      961 tccctaagcg aactaaaaaa agaaaggtca aggttcccga agcgatggac attctagttc
     1021 acgaagaggc acagaagctt attgatatgg acaaggttgt taaacaagca attgaaagag
     1081 tggagcagtc agggatcatt tttgtagatg agatggataa aatagcaggt cgtgaaaccg
     1141 ctgcgggacc tgatgtttca agagaagggg ttcaaaggga tctgcttcct ataatagaag
     1201 gatgcacagt aaatacaaag cacggaatgg tgagaactga tcacattctc tttattgctg
     1261 ctggagcctt tcatgtatca aagccttccg atcttattcc tgaacttcag ggtaggttcc
     1321 cgataagagt agaactcgac ccgttgacaa aggaagattt tatcagaatt cttacagaac
     1381 cccaaaatgc ccttattagg caatataagg agcttcttaa aacagagggg attgaactta
     1441 tattcaaaga cgatgcgata attgaggttg cagagatagc cgcatacgta aatgaatcaa
     1501 ctgagaatat aggggcaagg aggcttcaca cggttttaga aagaatcctt gaagacttat
     1561 cttttaatgc ccctgacata tcaaaggaaa gaatcgttat tgatacgaaa tatgtaaaag
     1621 agatgattgc agacattgta aaggatagag acttgagtcg gtatattttg tgaggaaata
     1681 ctttactgct gataatcaga caaattaaat tagcctgagt ttgacctgtg taaaactcta
     1741 atagacagaa ctctgaggat aaatatttat ctttaggtta ggagcttggt atagtgtaga
     1801 ttgactgatg ggtttagtaa aactacaacg tttaatttca tttgagtaca tttcataatc
     1861 agtttcagac attcgtgaat cgaaaacaat ttcattaaat aatgtatttg gatcgatatc
     1921 gaactctata atcttttgat cttttttaaa tctcccggtt gtttccctat aaactaacct
     1981 gacttccttt tcatgctcaa aagcttctcc tttaagcaac aaagtgagta ccggtccaag
     2041 acctgagtga tcgagtacag ttggagtaac aaaatcagga tcagcaaaca tttttcttat
     2101 ttcttcttct gtcatataca taactttgcc aatgtagtaa gagagactcg caaatttgtt
     2161 attcggatta taaatagctt cattaagtct tctaattgta gttttaactt ttgcgccgtt
     2221 cttatcagga gagtaaattc gccacatagc atccgattct tgataaagag tccagcactg
     2281 accataaaac ctatctgcta caccacgaaa gctcataatc ccgcctgaag gtaaaggggt
     2341 agagctcttg ataagaaagt tttcaaatgg atcgtcacac aactcgggcc ttactaaaac
     2401 caactttttg gtcttaaaca tctcgataag acgatttaca ttgaagactc gatagatgta
     2461 taggtctggg ttatcaatat tcacgaaatt gttcttcata cggttttgaa gttgcacctt
     2521 ggtcaaatgc tccataagtt atgtatacgg tctaaatgaa ataataaata ctgctacttc
     2581 aaaagaagta ataatgacta aacaaaactt ttattgcaag aaactttgtt tactatataa
     2641 taaaagtcta tcttttttga cgaccgatcg tgatgaaaga tctgattcaa aaagcgcaaa
     2701 cacttgttga agcacttcca tatataaggg aattttatgg aaagactctg gtgataaaat
     2761 acggaggaag cataggcgga agcgaactcc ataattttgc acgagacata gtgctaatga
     2821 aatacgttgg gatgcatcct gtggttgttc acggcggtgg tccacagatt ggaaatcttc
     2881 ttggaagact aggaataaaa tcaaagttta ttgcaggact cagggtcaca gatgccgaaa
     2941 ctatggaaat tgtagagatg gttcttgtcg gaaaggtaaa tcaggaaata ataactgcga
     3001 taaactccct tggtgggaat gcagcaggtg cttcgggtaa agatggaaag attatagttg
     3061 caaagaaggt tgatatatca aatatgattg atgatggagg agacatagca gaggatgcag
     3121 acttaggtat ggtaggagag gtggaaagaa taaatccaca actgataaaa gtccttgaaa
     3181 gcgcagggtt cattacagtg atagctccga tcgggttcga tgaggagggg aggtcttaca
     3241 atataaacgc cgatcatgtt gcagggaaga ttgcaggagc gcttcaagca gagaaattga
     3301 tactccttac agatgtacca gggatactgg atgaagaaaa aaacctaatt tcttcgctta
     3361 cagaatcgac tgcaagggaa cttatgaaaa atggaattat cacaagcggg atgataccaa
     3421 aagttagatg tgctattgag gctctccacg aaggtgtaaa caaagcccac ataattgatg
     3481 gaagggtaga acacgcatta atccttgaga tctttacaga tgccggtgta ggaactgaga
     3541 tactgctttg aaaacagaag agataatagc cagatttgat agatacgtga tgaataccta
     3601 cagccgtcac ccaatagtac tcgtgggtgg ggagggttgc tgggtaagag atattgaggg
     3661 taaaaaatat ctagacttta tcacaggaat cgcggtaaca aacctaggtc atagccatcc
     3721 tgctgtagtt aaagccatag aggatcaggc tcggattttg atccatgtat cgaatctatt
     3781 tcacatacag ccacaggttg aactagccga aattcttgta aagaattcat ttgccgacaa
     3841 gg
//
LOCUS       DATDRL010000354         3730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3565023, whole genome shotgun sequence.
ACCESSION   DATDRL010000354 DATDRL010000000
VERSION     DATDRL010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3565023"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..454)
                     /locus_tag="VI935_11875"
     CDS             complement(236..454)
                     /locus_tag="VI935_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26325.1"
                     /translation="MKHKNVETCRFCMKQIVKGEFRDTHIDGNLVCLDCYNGLNSTLE
                     NKKRYNAAYHYQTHRRPTLTGTPKARKT"
     gene            complement(509..1714)
                     /locus_tag="VI935_11880"
     CDS             complement(509..1714)
                     /locus_tag="VI935_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLE26326.1"
                     /translation="MAKDTELSSAFGKWPIDEFEESCGRDEIINPIEFGKVSLLDIAD
                     PEIEIQIETQKSGSLKKNRKERAPNEDIRLLQSYIKEVGQERLFTPKKEIETAAKIKS
                     CEARVIVINAALEKLLGTNLEQRYKELTRENLKVVLNGGSKTKVAKRLITLIELYSKT
                     VKALKARFIKANLRLVLIFTKRYLGRGLPLTDLIQEGNIGLMKAVDRFDHTRGYKFST
                     YASWWINQGMSRAILEQTGSIKVPVYVLEQATKVYKVSSMLSTENERKPLPEEISKKS
                     GLSLKWVKRVLESTKYAASLDAPIISDAETTLKEFIPDEHSSTADSVVVKTNLSVRIK
                     EALSTLNPREEKIVRMRFGIGYETEHTLDQVGKQFNLTRERIRQIERRALEKIEESEI
                     GIVLKSFLE"
     gene            complement(2094..2309)
                     /locus_tag="VI935_11885"
     CDS             complement(2094..2309)
                     /locus_tag="VI935_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010621601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HLE26327.1"
                     /translation="MKGTIKKLIKDRGFGFIRAEDGREIFFHRTAVQDANFESLTEGQ
                     SVEFDVEKNPKDSRTKGPRAVNVRVKA"
     gene            2884..>3730
                     /locus_tag="VI935_11890"
     CDS             2884..>3730
                     /locus_tag="VI935_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HLE26328.1"
                     /translation="MESDILNDVNPFQREAVTHHEGPLLALAGAGSGKTRIITYRIAY
                     LIKERGVSPTNILAVTFTNKAANEMKERVHRLVAKDAHELWIGTFHSVCLRILKKEID
                     KLDGFRRDFIIYDEADQIKLIRECMNKLGFSERFSDPRSVRSQIDCAKNKGQSPWDFG
                     TSIYDERISRVYHIYEQELRRANALDFGDLLDFTSTLFEKRPEVLDKYQNQFHHILVD
                     EYQDTNRLQYKIVKLLSEKHRNVFVVGDDNQSIYGWRGADITNILNFESDFPDAKVVK
                     LEQNYR"
BASE COUNT         1005 a          778 c          680 g         1267 t
ORIGIN      
        1 acctgctcgg gacacccgca aacttgcatc tatgtatgcg tcggcagtaa tacctgcgag
       61 attgccgcaa cctctcccgg tggattcgat agtatttgca ccacgggagg ggaatgtgct
      121 tcgggggggc tttttggagt tgtgggaggc gtcgtcattt attaacggct ggattgggaa
      181 agtagtcagg ctttatttat ttgattattc tccttgttgt gcttccttag actactcatg
      241 ttttccttgc ttttggtgtc cctgtcaagg tgggtcttcg gtgggtttgg tagtggtaag
      301 ccgcgttgta cctcttttta ttctcgagtg tgctgttaag cccattgtag cagtcaagac
      361 aaactaaatt cccatcgatg tgtgtatcac ggaattctcc tttaacaatt tgcttcatac
      421 aaaagcggca tgtttctaca tttttatgtt tcattcaacc tctttgattc agttgtttct
      481 tttagcataa atcaaaaacc ctcttcattt attcgagaaa gcttttaagc actattccta
      541 tctccgattc ttctattttt tcaagcgcac gccgttcaat ctgcctgatt ctctctctag
      601 ttaagttaaa ttgctttcca acctgatcga gcgtatgttc ggtttcatag ccgataccaa
      661 atctcatcct gactatcttt tcctctcttg gattaagcgt tgataaggct tctttaatcc
      721 tcactgaaag gttggtcttt actacaactg aatccgcagt agaagaatgt tcgtcgggta
      781 taaattcttt gagggtggtt tccgcatcac taatgatagg ggcgtcaaga gaggctgcat
      841 actttgtaga ttctaagact cgctttaccc attttaaaga taatcccgat ttcttagata
      901 tctcctcagg aagaggtttc ctctcatttt cggtggatag catggaactc accttgtaaa
      961 ccttggttgc ctgctcaagt acataaactg gcacctttat gcttcctgtc tgctcaagta
     1021 tagccctcga cattccttgg tttatccacc aggaggcgta tgtagaaaat ttgtatccac
     1081 gtgtatggtc aaacctgtca acagccttca taagaccaat attcccctcc tgaattagat
     1141 cagtaagagg gagtccacgt cccaagtatc ttttcgtaaa gattaaaact agtctgagat
     1201 tcgcttttat aaaccttgct ttgagggctt ttaccgtttt tgaatataat tctataaggg
     1261 tgataagcct cttcgctact ttcgttttgc ttcctccatt caagaccacc ttaaggtttt
     1321 ctcgtgttaa ttctttataa cgttgttcta ggtttgtacc aagaagcttc tctaaagcag
     1381 catttatcac tattaccctt gcctcacagc ttttaatctt tgctgcagtc tcaatttcct
     1441 ttttaggggt gaaaagacgt tcttgtccca cctctttgat gtatgattgt aggagtctaa
     1501 tatcctcgtt tggagctctt tctttcctgt ttttctttag agagccactc ttttgagtct
     1561 caatttgaat ctcaatttct ggatcggcta tgtccaacag cgataccttc ccaaactcaa
     1621 ttggattaat aatctcatcc cgtccgcaac tttcctcgaa ttcatcaatt ggccacttcc
     1681 caaacgcact actaagttct gtatcttttg ccattatctt atttagtcct tatcaacata
     1741 aaatttttga atcacctata attccatagt acgtttagaa tgcctatagt actgattcag
     1801 ctcattgatt taaagttgat gatcgttctg ccatctgaaa aatgtctctc atacggtctt
     1861 aaactttgcc ttcaccacag acgaataaaa atcccggatg aaaaccttac caacagggta
     1921 agagaatatc ttatgttttc ccacctcatt ctagatagat cgagggcctt gtctgtcatt
     1981 tccccttccc atatcatttt ttagttctat gcaattattt tctagaaatg tgttggaaaa
     2041 tacttccaag aagcaaaaag aaaacagaaa tgctcctctt ctatgaggag catttacgct
     2101 tttacacgca cgttcacggc acgaggtcct ttggttcgag agtcctttgg atttttttca
     2161 acatcaaact ctacggactg accctccgtt aaggattcaa aatttgcatc ttgaacagct
     2221 gtccggtgga aaaatatttc tcttccatcc tcagctcgaa taaatccgaa tcctctatcc
     2281 tttattaatt tttttatcgt tcctttcatg gctttgcccc ttttgtagga gtcatactag
     2341 atgacccctg attacacaca ttttatctat tattgttttt ttacaccctg cttatttctc
     2401 aatagctgtg tctgatctat ctagaccttc ctttcaatta aaaacagcta aatctgctat
     2461 ccctcttaac ctcaaccatt tactttaacg ataaactcta ctggattatt attattttat
     2521 caagtttttt cttttatatc taaaactatt ctacgccgtc cagacgcttc tcacgaaata
     2581 gaactcttga ataaacattc tcgggcaagt attaagggga taccaacgct atgcaaggga
     2641 tttttttacc cttgacatat tttaaatatc gtctatattc gatacgcaaa ggagttttcc
     2701 acaaatactt ttactgattt agtaaaaata attagggata tattattagg agtattatgg
     2761 tttggacata atgtgggaag ataagaaagg gtctataagc ttcccctgac tatttattgt
     2821 ctatctccca ttatatttgt atgggtttta atttatctgc ttttcaacat atgggggctt
     2881 aatatggaat cagatattct caacgacgtt aatccattcc agagagaagc tgttacacac
     2941 catgagggtc cgttgcttgc tcttgctggt gctggctcag gcaaaaccag aataataact
     3001 tatagaatcg cttatcttat taaggaaagg ggtgtatccc ctactaatat tctggctgtt
     3061 acattcacta acaaagcggc gaacgagatg aaagagaggg tgcatcgcct cgtagctaaa
     3121 gatgcacatg aattatggat aggtactttt cattcggttt gcctaagaat tctgaagaag
     3181 gaaatagata aactcgatgg ttttagaagg gattttataa tatacgatga ggcagatcag
     3241 ataaaactga taagagaatg tatgaataag ctaggattta gcgagcggtt ctctgatcca
     3301 agatcagtgc gctctcagat agactgcgct aaaaacaaag ggcaaagccc ttgggacttt
     3361 ggcactagta tctatgatga aagaatttcc agggtttatc atatctatga acaagaactt
     3421 agacgtgcga acgcacttga ttttggagac ctacttgatt ttacctccac tttatttgag
     3481 aaaagacctg aggttcttga taaatatcag aaccagttcc atcacatcct tgttgatgaa
     3541 tatcaggaca caaatcgcct tcagtataag attgtaaaat tgctctcaga aaaacataga
     3601 aacgtttttg ttgttggcga tgataatcaa tccatctatg gatggcgcgg ggctgatata
     3661 acaaacattc ttaactttga aagtgatttt cccgatgcaa aggttgtaaa gcttgaacaa
     3721 aactatagat
//
LOCUS       DATDRL010000355         2183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_148288, whole genome shotgun sequence.
ACCESSION   DATDRL010000355 DATDRL010000000
VERSION     DATDRL010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148288"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VI935_11895"
     CDS             complement(<1..245)
                     /locus_tag="VI935_11895"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HLE26329.1"
                     /translation="MRTYLLKRLFLIIPTLLGITLVTFFVIQLAPGNPVSRKLQVGEG
                     VKAEAITKDIIEQTKKLYGLDKPIYIRYWIWLKQIATL"
     gene            complement(328..1989)
                     /locus_tag="VI935_11900"
     CDS             complement(328..1989)
                     /locus_tag="VI935_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HLE26330.1"
                     /translation="MPHRSLSLLIILVFTTVLISCEPGGSETKSSSEEYSKYSDTEPV
                     EGDWLIYHIGAEPATLNPITATDAYQSIVNAGNIYETLVKRDNETLEIIPLLADRWEI
                     SEDKLVYTFFIKKGIEWHDGVPFTSHDVVFSYEKIMDPKVDAPHLRNYYQDIKRVEAL
                     DDYTVRFTYARPYFLALEFCGGMDIVPKHIFEKGDFNTNPAGRHPIGTGPYKFIKWDT
                     GKEIILERNESYWGKKLNIKRIIFKIITDPTVTLQVLKRGEIDFSGLTPIQWSRQTSS
                     ERFKKHFEKLSYFTPNYSFIGWNTNTPFFKDKRVRTAMTYLVNRKMILEKILYDLGEI
                     VTNPFYINSPEYDKSIKPYSFNPSMAKQLLDEAGWVDHDGDGIRDKEGVKFDFEFLLP
                     SGSVTGEQISTILKEELKKVGIRMNIRRLEWAVFTKQLDERKFDAVTLSWAMGVESDP
                     YQIWHSSQAERGSNFVGFRNREADQLIEQARTEFNRKKRIELYRRFAEIVYQDQPYTF
                     LFCRKSTVALQRRFKGVKVHPLGLDPLEWFVPLPLQRYGEVAMSY"
     gene            complement(2023..>2183)
                     /locus_tag="VI935_11905"
     CDS             complement(2023..>2183)
                     /locus_tag="VI935_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HLE26331.1"
                     /translation="LAEASDILSRNPMSLQLRYLQTLIEVSSEKNSTLVFPIPIDLLR
                     PFYEKANK"
BASE COUNT          627 a          457 c          409 g          690 t
ORIGIN      
        1 agagtagcta tttgcttcaa ccatatccag tagcgtatgt aaattggttt atcaagaccg
       61 tataactttt tagtctgctc tataatatcc ttcgtaatag cctcagcttt aactccttca
      121 ccaacctgga gctttctaga aacagggttt cctggggcaa gctgaatgac aaagaaggtg
      181 acaagggtta tacccagaag cgttggtata atgaggaata aacgttttaa taaataggtt
      241 ctcatattgt gattcgtgaa tcgtggctca tggttcgaat cacgaaacac taatcacgaa
      301 ccactccaca tagacaatct atatgattca gtaactcata gccacttctc catacctttg
      361 tagaggaagt ggtacaaacc actcaagtgg gtctaatccg agtggatgaa ccttaacccc
      421 tttgaatctt ctctgcaggg ctaccgttga tttacggcaa aagagaaagg tgtacggctg
      481 atcttgataa acaatttcag caaaccttct ataaagctct atcctctttt ttctgttaaa
      541 ttctgttcgg gcttgttcta ttaattgatc agcctctcta tttctaaatc cgacaaagtt
      601 agatcctctt tcagcttgag aggaatgcca gatttgatat gggtcagact ctacacccat
      661 agcccatgaa agggtaacgg catcgaattt tctttcatcg agctgctttg taaatactgc
      721 ccattcaagc cttcttatat tcatcctgat tcctaccttc ttgagctctt ctttcaatat
      781 cgttgaaatc tgctctcccg taacagaacc gctaggaagt aaaaactcaa aatcaaactt
      841 aactccttct ttatctctga ttccatcacc atcatgatcc acccatccag cctcgtcaag
      901 gagttgcttt gccatcgagg gattaaatga atagggcttt attgatttgt catactcagg
      961 gctatttata taaaatgggt ttgttacaat ctcacctaga tcatacagaa ttttttcgag
     1021 aatcattttt ctatttacaa gataggtcat tgcggttctc acacgcttgt ctttgaaaaa
     1081 aggtgtattc gtattccatc ctataaaact atagttcggt gtgaaatagc ttagcttctc
     1141 aaagtgtttt ttaaaacgct ccgatgaagt ttgtctcgac cactgtatag gagtaagacc
     1201 tgagaaatca atctcgccac gttttagtac ctggagtgta actgtagggt cggtaattat
     1261 cttaaatata attcgcttaa tattaagttt tttcccccag taactttcgt ttctctcaag
     1321 gattatctct tttccagtgt cccattttat aaacttatac gggcctgttc caatcgggtg
     1381 tcttccagca ggattggtat taaaatcgcc cttctcaaat atatgctttg gaactatgtc
     1441 cattccgcca cagaactcaa gcgccaaaaa gtagggccta gcatatgtaa accgaacagt
     1501 atagtcatca agggcttcaa ccctttttat gtcttggtaa taatttctaa gatgaggagc
     1561 atcaaccttt ggatccatta ttttctcata tgagaaaacc acgtcatgtg aggtaaatgg
     1621 aacaccgtca tgccactcga ttcccttttt aataaagaag gtgtagacga gtttatcttc
     1681 tgaaatctcc catctatcag ctagaagagg aatgatctca agagtctcat tatctcgttt
     1741 aacaagagtt tcataaatgt ttccagcatt cacaatagat tgataagcat ctgttgctgt
     1801 gatggggttt agtgtggctg gctctgcacc aatatgataa ataagccagt caccttcaac
     1861 aggctccgta tctgagtatt ttgagtactc ttcgctagat gattttgtct cgctaccgcc
     1921 gggctcacat gatatcaaaa ctgtagtgaa aactagaatg attagtaggg aaagggatct
     1981 atgtggcatc tataccgtac tttatcgaac aagaccagtt tatcacttat tagctttttc
     2041 ataaaagggt ctgagaagat caattggaat cggaaagaca agtgtggaat tcttttcaga
     2101 tgatacttcg ataagagtct gaaggtacct aagctggagc gacatcggat tccgagaaag
     2161 tatatctgag gcctctgcaa gcc
//
LOCUS       DATDRL010000356         6789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2264328, whole genome shotgun sequence.
ACCESSION   DATDRL010000356 DATDRL010000000
VERSION     DATDRL010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2264328"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VI935_11910"
     CDS             complement(<1..1277)
                     /locus_tag="VI935_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26332.1"
                     /translation="MKIPEGLDVAFNFGLLDSIFSRRSRRFGLGMEIKEGTLKHKSRF
                     EPIPLTELEEAFLIWAGTGITGLSLGDLPRTGISWLFQWTGRSWPCSCNSHSTELFYT
                     NDTGVYMVKLFNLMPEQGETAIFSGKSEEEKLDKILRLFRKSLIKLQDGRADLPKGEP
                     GLFDFNVWNTNKPGTTFFIPVTNITVEYMVLLFIYFGSKYGFNIIDELNGGRSCGLDK
                     WIEKGYIRNDVRMSLFDLELRVLTTLNVEQAFICQNMNLALQALGLGGWAFTGLLPHY
                     ALGVNPSYKGLGFRFIKPEKSLRSMLNPVPVGRDGFFEALCPPYVKDMNEAVDKFLKI
                     RGDFWKEGNPYPYQNPQGVLQDEYHPSEVRIQIVKDFCTYVYENFGRFPAFLDPMFAR
                     LVFQAHHLDLEFYDKHYTEGAYTEVHRNHFKLW"
     gene            1542..2951
                     /locus_tag="VI935_11915"
     CDS             1542..2951
                     /locus_tag="VI935_11915"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HLE26333.1"
                     /translation="MPLTSTEVGFIFIILVLLFFLFSLPSKTIAQAQSEADERSKEIE
                     RIKSEIQRIKEEQQKQIEELQKRIGELEAETQKEREKEYVEPSKPPKEVEEAPAKTYV
                     IEWWKNIEVGYSNGFFIRTQDDLFSLKFNIRSQFLFFVEDEGDDTATSFDIRRLWISF
                     RGNAFRPWFKYLLILEASGDVELLDYFMDAAKITAVVPRAGQYRVPFNREELTDPFNL
                     QLVDNSILNREFSLGRHIGVGLGGVGNRFVTYGVGVFNNAGRNSINENSNLLYVGRVM
                     FTPTGEPRYSSINPFPVSGEYAYSQGSFGDTETPLIAISAAVAFLPGYKPVNDSPDNV
                     VINNRMFALGSDKSNIFQFSADISVKYSIFSLEAEYDLRNINPDEAGIDSALSQGVRV
                     QSGVFLIPKLIEVAGRFAIIDLGNDFGDDKIWEITPGLSFYFIKNHNLKLQFDYSFIR
                     DELLDTDINRARAQLTVSF"
     gene            2999..3712
                     /locus_tag="VI935_11920"
     CDS             2999..3712
                     /locus_tag="VI935_11920"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HLE26334.1"
                     /translation="MKKWIIVLTSSILTFCISISGYTQNSKSTKGYGKITGTVKVFEK
                     NLLRDFKEKDDLSGVIVYLTGLETKPPKEVPNLVQQNKAFNPTLLPIVAGQTVSFPNH
                     DGIYHNVFSVSPVKSFDLGQYKSTDPPKDITFDKAGLVPVFCNIHPQMISFVLVLENN
                     AFGESDKEGKFVIPNVPPGSYTINAWKPKTQRMSSEVEVLPGQETVINFELKEVEKIP
                     PHKRKDGSNYPGYQDDWES"
     gene            3713..5761
                     /locus_tag="VI935_11925"
     CDS             3713..5761
                     /locus_tag="VI935_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLE26335.1"
                     /translation="MFSKLPLSFRTKIFLVLGAIVFLSIATVLLVLQQTTKNRVEENI
                     KKRFDSTIYAFRELQELRTQFASDEINSLTMSNPQFRTILSTASVGTEDFGFGAPTDH
                     NEVLKDANLRLNSILPFLPIYQKSDIFLVTNSEGELLFTKADPRKFGNELSDLSLFRK
                     TFEEGEAEGIWSSRLFDEKGVKIFPQGVKESIYRLVAKPVAFGEEIHGVVFWGSRIDK
                     KTLESIKEISSVDIALYSKEDINATTLPPEKEEELSKLVSSKILEFDKGDKDFVSEVS
                     LGKERFIIRWASILPGMPMQQSGFIVLKSLTEELSFLRKLQITLLIIGVTILVVAIGL
                     SFLVAGGVTKPVRALASAAKKIGKGELETKVDIRTGDELEELGGAFNEMVKGLKEREF
                     IKSTFERYVSKTVASELIKNPEMVRLGGIKKELTVMFSDIGEFTNLSETLSPEEVVKH
                     LNDYFEGMSSSILEFDGTINQFQGDAIVAFWGAPIPQDNHAILACLAALRCREFLKNL
                     QAKWVSQGLPERTFRFGINTGEMVVGNIGSSSRFEYTVIGDEVNLASRLEGANKIYGT
                     QILISDGTYKFVKEEVIARELDIIRVVGKTEPVRIYELVSQKGELDDKKMGVIEKFSL
                     GVDLYRERKWNEAKEYFEKILELDPSDKPSQEYLRRCIEYENSPPPGEWEGVFDLKSK
                     "
     gene            complement(5794..6078)
                     /locus_tag="VI935_11930"
     CDS             complement(5794..6078)
                     /locus_tag="VI935_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26336.1"
                     /translation="MKKKKKLTEEKIDEIVTKEADDLTRWEEPILVKPTNVTSIRLSP
                     ETIRKAKYLAKIHKFRGYQTWLKQIIEERIRLEEVILTDLKQEFRKDSSR"
     gene            complement(6075..6323)
                     /locus_tag="VI935_11935"
     CDS             complement(6075..6323)
                     /locus_tag="VI935_11935"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HLE26337.1"
                     /translation="MDWSRFIPFDFEYDWEKDKLAKHGVSFEEAVETFFNGYEVRRNK
                     RFRDRYQLIGRTNGGRRLKIVFQLKPRNVVRIITGWEV"
     gene            6429..>6789
                     /locus_tag="VI935_11940"
     CDS             6429..>6789
                     /locus_tag="VI935_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLE26338.1"
                     /translation="MAVRSRLVRKSIHLNINLANRLEGANKIYGTQILISDETYKLAK
                     EEVIARELDIIRVVGKTEPVKIYELVPQKSELDEKKTSVLDKFQVGVNLYREQKWVEA
                     KEYFEKILELDPSDKPSQ"
BASE COUNT         2074 a         1369 c         1410 g         1936 t
ORIGIN      
        1 tgccagagtt taaagtgatt tctatgaacc tctgtatatg ctccttccgt gtaatgcttg
       61 tcatagaatt ccagatcaag atgatgggcc tgaaaaacta atcttgcaaa catgggatca
      121 agaaaggcag ggaatcttcc aaagttttca taaacatagg tgcagaaatc ctttacaatt
      181 tgtattctca cctctgaggg atggtattca tcctgaagca ctccctgtgg attctgatat
      241 ggatatggat ttccctcttt ccagaaatcc cctctaattt taagaaattt atctactgcc
      301 tcgttcatat ccttaacata agggggacag agcgcttcaa aaaagccgtc tcgccctact
      361 ggaaccggat tcagcatgct ccttagactt ttttcaggtt ttatgaatct gaaacccaat
      421 cctttgtaag aggggttcac acccaatgca tagtggggaa ggagtccagt aaatgcccat
      481 ccgcctagtc caagtgcttg aagggcgaga ttcatatttt ggcagataaa agcctgttca
      541 acattcagcg ttgtgagaac acgaagctca aggtcaaaga gagacattct cacatcgttt
      601 cttatataac ccttctcaat ccatttatct aaaccgcagg acctcccacc gtttagctca
      661 tcaattatat tgaacccgta ttttgagccg aagtagataa aaagaagaac catatactca
      721 accgttatgt ttgttacagg aatgaaaaag gtagttccag gcttattcgt attccatacg
      781 ttgaaatcga aaagccctgg ctcgcctttt ggcaaatccg ccctcccatc ctgaagcttt
      841 atcaaactct ttctaaagag acgaagtatt ttatctagtt tttcttcctc tgatttgcct
      901 gaaaaaatcg ctgtctctcc ctgctctggc atgaggttaa agagttttac catatataca
      961 cctgtatcat tcgtgtagaa aagctcagtt gaatgcgagt tgcacgaaca gggccagcta
     1021 cgtcctgtcc attgaaaaag ccaagagata ccggttctag gtaagtcacc cagcgaaagc
     1081 ccagtgattc ctgttccagc ccagattagg aatgcttctt ctaactcagt taggggtatt
     1141 ggttcaaacc tggatttgtg ttttaatgtg ccttctttta tctccattcc caaaccaaat
     1201 cttcttgaac gccttgaaaa tatagaatca agaaggccga aattgaatgc aacgtctaac
     1261 ccctctggaa ttttcatagt tttctcctcc agcttatacc gtaatacaat tgagtgttat
     1321 acatcaaggg gggattgctt cgtcacttcg ttcctcgcaa tgactcaaag gatgtcattg
     1381 cgagccgaag cccagggcct agcgaagggg cagcgaagca atctcatctt tttcaaccat
     1441 ctgtagcaca cccaactgta ggctgctata atttgctaca tatgattgta tacaataaga
     1501 tgtatgaata aaactaatag acctcactct gaaccatgcg aatgccgcta acgtcaacag
     1561 aggtaggatt cattttcata atccttgtgc tcctcttttt cttattttct ttaccctcta
     1621 aaaccattgc acaagcccaa tccgaagccg acgaaaggag taaagaaatt gaaaggatca
     1681 aatccgagat acagaggatt aaagaggagc aacaaaagca aatagaggaa ttacaaaaac
     1741 gaattgggga acttgaggct gagacccaaa aggaaaggga aaaggaatat gttgagccaa
     1801 gtaagccgcc taaggaagta gaggaagccc ctgcaaaaac ctacgttatt gagtggtgga
     1861 aaaatattga agttggttac agtaatggat tttttatcag aacgcaggat gatttattct
     1921 cccttaagtt taacatccgt tcccaattcc tcttctttgt cgaagatgag ggagatgata
     1981 ctgcaacaag ttttgatata agaaggttgt ggatctcttt tagaggaaat gcatttcgtc
     2041 cctggtttaa gtatttgttg atactcgaag caagcggtga tgtggaactt cttgattatt
     2101 ttatggacgc ggccaaaatc actgctgtag ttccaagagc gggacaatat agggttccct
     2161 ttaatagaga ggagctgacc gaccctttta acctccaact tgttgataac tctatcctca
     2221 atcgtgaatt tagcttaggt cgtcatatag gtgttgggtt aggaggtgta ggtaatagat
     2281 tcgtcacgta cggcgtcggt gtattcaaca acgctggaag gaattctata aatgaaaact
     2341 ccaacctgct ttatgttgga agagttatgt tcactccaac cggagaacct agatactcgt
     2401 ctattaaccc attcccagtt tcaggggaat acgcttattc gcaaggctcc tttggagata
     2461 cagaaacacc cctaattgct ataagtgccg ccgtagcgtt tcttcccgga tataaacccg
     2521 tgaacgatag ccctgacaac gttgttatta ataataggat gtttgcctta ggatctgata
     2581 aaagcaatat atttcagttc tcagccgata tttcggttaa atattcaatc ttttccctcg
     2641 aggcagaata cgatctgaga aatattaacc cagatgaagc aggcatagac agtgcgcttt
     2701 cacagggagt tagagttcag tccggcgtgt ttcttattcc taagcttata gaggttgcag
     2761 gaaggtttgc tatcatagac ctgggtaacg attttggtga tgacaaaatc tgggagatta
     2821 cacccgggct tagcttttat ttcataaaga atcataactt aaagcttcag tttgattatt
     2881 catttattag agatgagttg cttgatacag acataaacag ggcaagggct caacttacag
     2941 taagttttta aacgtatcgt taagaatggt ataattttat aatttcaaga gagtctgaat
     3001 gaagaagtgg attattgttt taaccagctc aattttaaca ttctgcattt ctataagcgg
     3061 ttacacacag aactcaaagt ctactaaagg ctacggtaag ataacaggca ctgtaaaggt
     3121 ttttgaaaag aatttactaa gggacttcaa agagaaagat gatctgagcg gggttattgt
     3181 ttaccttacc ggacttgaaa ctaaaccccc aaaagaagtt ccaaatctag tccagcaaaa
     3241 taaggccttt aaccctacgc tcctcccaat agtcgccggg cagactgtta gcttccctaa
     3301 tcacgatggt atatatcata atgtcttttc tgtatcgccc gttaagtcct ttgacctcgg
     3361 tcaatacaaa tctactgacc cgccaaagga tattactttc gacaaagccg gtttagttcc
     3421 tgttttctgt aatatccacc cccaaatgat ttctttcgtt cttgtacttg agaacaacgc
     3481 ctttggggag tccgataaag aaggcaaatt cgttataccc aacgtaccac caggtagcta
     3541 tacgataaac gcatggaaac ccaagaccca gaggatgagc agcgaagtag aagtccttcc
     3601 aggacaagaa acagtaataa attttgaact caaagaggtt gaaaagatac cacctcacaa
     3661 gagaaaagac ggctccaact atccaggata tcaggatgat tgggagtctt aaatgttttc
     3721 taaactaccg ctcagcttca gaacaaagat tttcctcgtt cttggcgcga ttgttttcct
     3781 atctattgca accgttctcc tagtccttca acaaacgaca aaaaaccgtg ttgaagagaa
     3841 cattaaaaaa agattcgata gcactatata tgcctttaga gaactccagg agctcagaac
     3901 tcaatttgcc tctgacgaga ttaactcgct cacaatgagc aaccctcaat ttcgcactat
     3961 actttccacc gcatccgttg gaactgagga ctttggtttt ggagcaccta cagaccacaa
     4021 tgaggtacta aaggacgcaa acctcaggct taactcaatc cttccatttc tacccatata
     4081 ccaaaaaagc gatatttttc tcgtaaccaa ctctgagggt gagcttctct ttactaaagc
     4141 agacccaagg aaatttggca acgaattaag cgacctctct cttttcagaa agacatttga
     4201 ggagggagaa gcagagggta tttggagctc acgcctattt gatgaaaaag gggtaaaaat
     4261 ttttcctcaa ggagttaaag agtctatcta tcgattagtt gcgaaacccg tagcctttgg
     4321 tgaggagatt catggagtag ttttttgggg aagcagaata gataaaaaga cacttgagag
     4381 tataaaggaa atatcctccg ttgatatagc cctctactca aaagaggata ttaatgctac
     4441 aacccttcca cctgaaaagg aagaagaact atcaaaactt gtcagctcga agatattaga
     4501 gtttgacaag ggagataagg attttgttag tgaagtatca ctcggtaaag aaaggtttat
     4561 aattaggtgg gcatccatcc ttccagggat gccaatgcaa caaagtgggt ttatagttct
     4621 taaatccctt accgaagaac ttagttttct tagaaaacta caaatcaccc ttctgataat
     4681 aggagttaca atcttagtgg tagctatagg tcttagcttt ctagtagcag ggggagtcac
     4741 aaagccggtt cgggcccttg cttcagctgc gaaaaaaatt ggaaagggag agcttgaaac
     4801 aaaggttgac attcgtacgg gcgatgaact tgaggagctt ggtggagcat tcaatgaaat
     4861 ggtgaagggt ctaaaggaga gggaatttat caaatctaca tttgagcgat atgtttcaaa
     4921 gacggttgct tcagaactca ttaaaaaccc tgagatggtg cgtcttggcg ggataaagaa
     4981 ggagcttacg gtaatgtttt ctgacattgg agaattcact aatttgtcag agacattatc
     5041 acccgaggaa gtggtaaaac atcttaacga ttatttcgaa ggaatgagct cttcgattct
     5101 cgagtttgac ggaactatta atcagttcca gggtgacgca atagttgctt tttggggagc
     5161 accgattcca caggacaacc acgctattct cgcctgtctt gcagcactca gatgtcggga
     5221 gtttctcaaa aacctccaag ctaaatgggt atcacaagga cttccagaga ggacatttcg
     5281 ctttggaatc aacacgggtg agatggtagt tggtaacatt ggttcttcct cccgctttga
     5341 atacacggta ataggggatg aagtaaatct ggcaagcagg ttggagggag cgaataaaat
     5401 ttatggaact caaattctta tttctgatgg aacctacaag tttgtaaaag aagaagtcat
     5461 agcaagagaa ttagatatta ttcgcgttgt aggaaaaaca gaacccgtaa gaatctatga
     5521 actcgtttca caaaaagggg aattagatga taaaaaaatg ggagttatag agaaattcag
     5581 cctaggtgtt gatctctaca gagaacgtaa gtggaacgag gctaaagaat attttgaaaa
     5641 aattctggaa ctagacccat ccgataaacc ctcccaagaa tacttgagac gttgtattga
     5701 atacgaaaac tctccaccac ccggtgaatg ggaaggagtg tttgacctaa agagtaagta
     5761 atcctctcac cagattgatc gtctttattg gcattatctg ctgctatctt ttctgaattc
     5821 ttgcttgaga tcagtgagaa taacttcttc taaacgaatt ctctcctcta tgatttgctt
     5881 taaccaggtc tgataacccc taaatttatg gatcttagcg agatacttgg cttttctgat
     5941 tgtctcagga gaaaggcgga tggaagtcac attggtgggc ttgacgagta tgggctcctc
     6001 ccaccttgtg agatcgtctg cttctttcgt aacaatctcg tcaatttttt cttcggtaag
     6061 ttttttcttc ttcttcatac ttcccatcct gtaataatcc gaactacatt tctgggcttg
     6121 agttggaaaa cgatttttaa ccttcttcct ccgtttgtcc ggccaatgag ttgataacga
     6181 tcgcggaatc ttttgttcct tctgacctca tagccgttga aaaaggtttc caccgcctct
     6241 tcaaagctta ctccgtgttt tgcaagctta tccttctccc agtcatattc gaagtcaaag
     6301 ggtatgaagc gactccagtc catctatgtc tcattgttgt ttgtaaatac atattaccag
     6361 aaaatagaag agtttcaaaa agattacatt acttagtgta gctacatatt ctagctcctt
     6421 tttgaagtat ggcagttaga tcacggctag ttcgtaagtc catacacctt aacataaatc
     6481 tggctaacag gcttgaggga gcgaataaga tttatggaac tcaaattctt atttctgatg
     6541 aaacctacaa gcttgcaaaa gaagaggtca tagcaaggga attagatatt attcgtgttg
     6601 tgggtaaaac agaacctgta aaaatctacg aactcgttcc gcaaaaaagt gaattagatg
     6661 agaaaaaaac ttcggtctta gataaattcc aggtaggggt taatctctat agagaacaga
     6721 agtgggtcga ggctaaagaa tattttgaaa aaattctgga actagaccca tccgataaac
     6781 cctctcaag
//
LOCUS       DATDRL010000357         2365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_686437, whole genome shotgun sequence.
ACCESSION   DATDRL010000357 DATDRL010000000
VERSION     DATDRL010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686437"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="VI935_11945"
     CDS             <1..1290
                     /locus_tag="VI935_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLE26339.1"
                     /translation="GEVEKRVVAQLLALMDGLKDRGRVIVVGATNLPSMLDSALRRPG
                     RFDREVSLDVPDRNGRLEIFEVHTRGMPLADDVDLERLADITHGFVGADIENLCREAA
                     MGSIRTVLPEVDFEGGNLPYDKLMELRVEMEDFIDALRGVEPSAIREIFVEVPKVNWK
                     DIGGLDEIKERLTEAVIWPIKYRELFEATQTKAPKGILLYGPPGNGKTLLAKAIANES
                     GVNFISIKGAELLSKYVGESEHAVREVFKKAKQVAPCIVFFDEIDAVAPCRAGGDNTH
                     VSERVVSQLLIEMDGVEELRGILVLAATNRIDMVDPALLRAGRFDLSLIIPYPDEEAL
                     FEILLIHTRGKPLARDVNLREIAGSIKGLSGADVELLCQRASLIAIREHLNRGKGVLR
                     ITHLHFEEALKETGGRISGPILPPNISKGKFGGETLS"
     gene            1302..2150
                     /locus_tag="VI935_11950"
     CDS             1302..2150
                     /locus_tag="VI935_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HLE26340.1"
                     /translation="MEQTIASIGSSALIIPAFIFGSVVGSFLNVCIYRLPRRESIIFT
                     HSHCTSCGAPIKLYDIIPIASYLILFGRCRACGSRISPTYLVVELLSGVVSALLVLRF
                     GISLTSLFYLIFLFSLIVVTFIDLEHRIIPNVITLPGIIVGLIYNALNTNWVTLQMEL
                     SYFQFTLSDFLRISDKVDIIGSILGIILGGGILYFIGFIYEAIKKREGMGMGDVKLLA
                     MIGAFIGWKGVIFVAFLSSLFGVLVGIPIIFFKKGDLKYAIPYGPFLSLAAALYCFTG
                     GFSFRF"
BASE COUNT          669 a          410 c          549 g          737 t
ORIGIN      
        1 ggggaggtgg aaaaaagggt tgtggcccag cttcttgcac ttatggatgg gctgaaggat
       61 agaggtaggg taatagtagt tggggcgaca aatcttccga gcatgcttga ctctgctttg
      121 agaagaccag gaagattcga tagggaggta agtttagacg tccctgatag aaatggaaga
      181 cttgagattt ttgaggttca tactcgcggt atgcccctag ccgatgatgt agacctcgaa
      241 cgccttgctg atatcacaca tggctttgtc ggtgcagaca ttgaaaacct ctgccgcgag
      301 gcagcgatgg gatctattag gacggtgcta ccagaggtag attttgaggg cggtaatctt
      361 ccatatgata agctcatgga acttcgggtt gagatggaag attttatcga tgcattaaga
      421 ggagtcgagc catcagcgat cagagagata tttgtcgagg ttcctaaagt caattggaag
      481 gatataggtg gccttgatga aataaaagag aggcttacgg aagccgtcat atggcctata
      541 aaatataggg agctttttga agcaacacag acgaaagccc caaaaggaat ccttctctat
      601 ggaccgcctg gaaatggaaa aacccttctt gcaaaggcta tagcaaatga aagcggagta
      661 aattttattt cgataaaggg tgcagaactc ctttcaaaat atgtaggcga gtctgaacat
      721 gctgtaagag aggtgtttaa gaaggcaaaa caggttgccc catgcatagt cttctttgat
      781 gagattgacg ccgttgcccc ctgccgtgct ggtggcgata acacacatgt ttcagaaagg
      841 gtggttagtc aactactgat agaaatggat ggcgtagaag agctaagggg tattctcgta
      901 ctcgctgcta caaatcggat tgatatggta gatcctgcgt tattgagggc cgggagattt
      961 gacctatctc tgataattcc ataccctgat gaagaggcgc tgtttgaaat cctgctgatt
     1021 catactcgtg gaaaaccact tgcaagggat gtgaatttaa gagagatagc tggaagtatt
     1081 aagggtttat ctggtgccga cgtcgagcta ctttgtcaaa gggcttcgct tattgcaata
     1141 agggagcact tgaatcgtgg aaagggtgtt ttaagaatca ctcaccttca ttttgaagag
     1201 gccttaaaag agacaggtgg aaggatatca ggacctatac ttcctccaaa catttcgaag
     1261 ggaaagtttg gaggagagac tctctcataa ctctaaacca attggaacaa accatagctt
     1321 ctatcggttc ttcagcactg attatccctg ccttcatttt cggttctgtt gtagggagtt
     1381 ttcttaacgt ctgcatctac agattaccaa ggcgcgaatc gataatcttt actcattctc
     1441 actgcacgtc ttgtggcgct cccatcaagc tatacgatat tatccctata gctagctacc
     1501 tcattctttt cggaaggtgt agggcctgtg gctcaaggat ttctccaacg tatttggttg
     1561 tagagcttct ttccggtgtt gtttctgccc tcttagtctt gagatttggt atatctctaa
     1621 catctttatt ttacttaatt tttctttttt cacttatcgt cgtgactttt atagatttag
     1681 aacataggat aattcctaat gtaataactc ttcctgggat catagtaggg cttatttata
     1741 atgctttgaa taccaattgg gtaacattgc aaatggaact tagttatttt cagtttacat
     1801 tgtcagattt tcttagaatc tctgataaag tagatattat tggctcaata ttagggataa
     1861 ttcttggcgg aggaatactc tattttatcg gatttattta cgaggctata aaaaaaagag
     1921 aaggaatggg gatgggtgat gtaaaacttc ttgcgatgat tggggctttt attggctgga
     1981 agggagtaat ttttgttgca tttctaagct ccttatttgg agttcttgtt gggataccca
     2041 taattttttt taaaaaaggc gacttaaaat atgccatccc atatggtcca ttcctctctc
     2101 tggctgctgc attgtactgc tttaccggag gatttagttt taggttttag ttgaacagtt
     2161 tttcatatat aagacaattc aaattaagtt taaaacctat aaaattaagg aagttgactt
     2221 atggaaaagc ttactcatgg agttaataac caatcccttt gtagcgacta ctatcttacc
     2281 aaacttgtta atgttgacga tttctttaag aaaaatacga ctaaatagtg attgttaatt
     2341 aaataattta aaattatttt attaa
//
LOCUS       DATDRL010000358         2841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1996608, whole genome shotgun sequence.
ACCESSION   DATDRL010000358 DATDRL010000000
VERSION     DATDRL010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996608"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VI935_11955"
     CDS             <1..643
                     /locus_tag="VI935_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26341.1"
                     /translation="IMKCILCEQRKAKRYCPAKRTYICAICCGEKRGVEINCPLDCVY
                     YIEGQKYQQDKITRQRIMKEGTEPYIRRAELYSKNPEIFAKIELALVGFFRNDNKLKN
                     EEVAEALELVLKTLDTEKKGILYEYRAESSVVNEVALRVLTVIREYKDMAELRRGRIT
                     LDYAKDVIEEFLKEIKFYMEIERNPQSYLIHISRYHPERVETRQGGGSLIISS"
     gene            complement(1001..1180)
                     /locus_tag="VI935_11960"
     CDS             complement(1001..1180)
                     /locus_tag="VI935_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26342.1"
                     /translation="MPKTNPIGSVIICLVLLSLIGYAFSRALNLDWKLYFTVAGIIVL
                     IAAITGIAAAGGNKS"
     gene            1985..>2841
                     /locus_tag="VI935_11965"
     CDS             1985..>2841
                     /locus_tag="VI935_11965"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HLE26343.1"
                     /translation="MYDENEFKISVIGAGSWGTTIAKHLAEKGFEITLWVKEERLRES
                     ILRTRENHLYLPKIKLPDNIHPTNLLWEALTGRDLIIFSVPSQYVRGVLEDGSRFIRD
                     DSLIINTAKGIELTTHKLMHEVFEEVLSQGIRERYASLSGPSFALEVAKHKPTAVSIG
                     SKNLNAGKIGQKVFSNEYFRAYVTEDVVGVEIGGSLKNVIAIAAGIADGIGLGYNSKS
                     ALITRGLAEITRLGLKMKANPMTFLGLSGVGDLVLTCTGELSRNREVGVRIGKGERLE
                     DILSNMRMVA"
BASE COUNT          905 a          526 c          653 g          757 t
ORIGIN      
        1 aatcatgaaa tgcatccttt gtgaacaaag aaaagctaaa agatattgtc ctgcaaaaag
       61 aacgtacatc tgcgcaatat gttgcggtga aaaacgtggg gttgagataa attgccctct
      121 ggattgtgtc tattatatcg agggccaaaa atatcagcag gataaaatca caaggcaaag
      181 aataatgaaa gagggcaccg agccttatat cagacgtgcc gaactatata gcaagaaccc
      241 tgagattttc gctaagattg agcttgctct agttggtttc ttccgtaatg acaataagct
      301 taaaaacgaa gaggtcgcag aggcgcttga gctcgttctt aaaaccctag acacagagaa
      361 gaaaggaata ctatatgaat acagggctga aagcagtgtt gttaatgagg ttgcgttacg
      421 ggtgttaact gttataagag aatataaaga tatggccgaa cttcgtaggg gaagaataac
      481 gcttgattat gcaaaggacg taattgaaga gtttttaaag gaaattaagt tttatatgga
      541 aatagaaagg aatcctcaaa gctacttaat ccacatctca cggtatcacc ctgaaagggt
      601 tgaaacaaga caaggcggtg gttcattaat tatctcttct taaatattaa tccacctttg
      661 aaaaggggga ttttgaaaat tctccccttt tttaaagggg agttggaggg gattccacac
      721 cattacaaat ggggttatat tttaaaattt tagtgcaact ttaatggact attacctaat
      781 tcaattcaat aaccctgcag caagctgcag ggtatggtct taaaaatata ccgttatagc
      841 tgtcattgcg aggagcaaag cgacgaagca atctaaaatg agagattgct tcacttcgtt
      901 cgcaatgaca atacactatt atggaaaccc tgtagcaagc tacagggaat tctcaagttc
      961 aagctatctt acaaaagcaa aaggaaaaag acaaacaagc ttatgactta ttaccgcccg
     1021 ctgctgcaat gccagttata gcagcaatta gaacgattat cccggcaacc gtgaaatata
     1081 acttccaatc tagattgaga gcacgggaga acgcataacc aatcagtgaa aggagtacca
     1141 agcatattat aacgcttcca attgggtttg tttttggcat ctctgatcct ccgatgttct
     1201 tgatccagta tgcacgtgat tttttttcag agaaatattc tatctggatg tgaagtaata
     1261 tacaaatttg ttggtaaatg ggagatagtc tacaatgtca atttgatttt tcgatattgc
     1321 ataacgaagc ccgtaagtaa tcttccgacg gagtttacac tgagcaagaa atcccctcta
     1381 tctccccttt gagaaagggg agaaaaacta taatcaccct ttgtaaaggg ggatttagtg
     1441 gggtatttaa taaaggctgt ggatggggat aaaagtgaaa agccgacccg actttggccc
     1501 ttagcagttc gacagttaga ttcttctgga cactcagggc tcactcctca ctacgagctt
     1561 agtcgaacgg taaactccgt gacgaagcaa tcccatggtt tatcctgtca tcgcgtgtaa
     1621 cgaagcgatc tgacgcatgg cgtcatcctg aaggagcaaa gtgactgaag gatctcttga
     1681 tttatcttgg gatccttcgc cagactcttc acggagttta tcctgaggta tcgaaggatc
     1741 agagcttcgg ctcaggacga cattcagtgt cggattgctt cgtccttcca ctcctggcaa
     1801 tgacaatcag gggcagggga ttgccacccc ctcgcttcgc tctgggcttc ggctcacacg
     1861 ctcgcagcaa ctcctttttg aacgcactac tgattgatag aatttggaac gaacaaaaga
     1921 actatgagtt ctagtcatct cactcttgaa ccattatatc aaatatgtaa acattattga
     1981 tactatgtac gatgaaaacg agtttaaaat tagcgttata ggggctggaa gttggggaac
     2041 aactattgca aaacaccttg cggaaaaggg ctttgaaatc actttatggg taaaagagga
     2101 gagactaaga gaatcaattt taaggacaag ggaaaatcat ctatatcttc ctaagataaa
     2161 acttcctgat aacatccatc caaccaacct tctatgggag gcattaacgg gcagggacct
     2221 gattatattc tcggttccat ctcaatatgt aaggggggtt ttggaagatg ggtcaagatt
     2281 cattagagac gattctttaa tcattaacac agcgaaggga atagaattaa ctacacataa
     2341 gctcatgcac gaggtttttg aggaggtgct ctctcaaggc attcgtgaaa gatatgcttc
     2401 actctcaggt ccgagttttg cactagaggt agcgaagcat aaaccaacag ccgtgtctat
     2461 tgggtcaaag aaccttaatg cggggaagat cggacagaag gttttctcaa acgagtactt
     2521 tagggcttac gtgacagaag atgttgtagg agtagaaata gggggctcac tcaaaaacgt
     2581 aatcgcaatt gcggcaggga tcgcagatgg cattggactt gggtacaatt caaagtccgc
     2641 tcttatcaca agaggacttg ctgaaataac tagattaggg ttaaaaatga aagcaaaccc
     2701 tatgacattt ctaggactat ctggggttgg agatcttgtt ctaacatgta ccggggagct
     2761 tagtaggaat agagaggttg gggtaagaat tggtaagggc gagagattag aagatattct
     2821 ttcaaacatg agaatggtag c
//
LOCUS       DATDRL010000359         2690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3761824, whole genome shotgun sequence.
ACCESSION   DATDRL010000359 DATDRL010000000
VERSION     DATDRL010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3761824"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VI935_11970"
     CDS             <1..656
                     /locus_tag="VI935_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26344.1"
                     /translation="EMIPVSDDGSTHTTVFVPAGTNRLFEAKFIDGDGILMAFCSAFA
                     DINFGVENNVVLPCSAFVCTECDDNPIVETVCDGERCDFENETKVCVNGECITPTPPP
                     TPSPTPTPAPTPTPTPRPILGCCDLGQTCANSSNLQCADMRGFFFPGAMCFANGCSTT
                     PPTPPPTPTPPPFPVCCEYDDTCAENSNVECDMLGGTSFFPASCYNCDGSCIFFCGD"
     gene            complement(1160..1342)
                     /locus_tag="VI935_11975"
     CDS             complement(1160..1342)
                     /locus_tag="VI935_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26345.1"
                     /translation="MNKKSKVIYSINIEDLQTVAEQELERELTDEEIKDVEKRLGDYI
                     DWYGAIASTLNEAIKK"
     gene            complement(1417..2286)
                     /locus_tag="VI935_11980"
     CDS             complement(1417..2286)
                     /locus_tag="VI935_11980"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HLE26346.1"
                     /translation="MQQFWLLVGSEKNWKVAFENKNLWGLKDFRELRSLWNMLREGDG
                     LLFYVSKPVHGIVGFGHVVTKFRQTNPLWPEEIKRNEVVWPLRFEFDIEYCLPPSLWK
                     SHRYTSHNLQMISRMVFQCYPIEEVNAARISLDLKLLTETTSLPPTEFSISTETTTLK
                     HDDVKAYLAEIGRVQGYIADEEYPLESTRLDVVWRRVERSVPTFVFEVQIGGDIYHAM
                     AKLKHAYDLWNSHIFLVAAMGDKSKYQELLSGTFHEVAKQMRFIEVSSVKELLGKKRD
                     YKEMERGLGIFKR"
     gene            complement(2333..>2690)
                     /locus_tag="VI935_11985"
     CDS             complement(2333..>2690)
                     /locus_tag="VI935_11985"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLE26347.1"
                     /translation="KLRVTYQDACHIAHAQRIKEHPRKIIKHIPGIEFVELPESDLCC
                     GSAGIYNLIEPDMAEKLLERKISKLKETGVQVLVAGNPGCLLQIGMGIKRHGLNIKTA
                     HPVELLDWAYQGRNYF"
BASE COUNT          775 a          591 c          493 g          831 t
ORIGIN      
        1 tggaaatgat tccggtttct gatgatggtt ctacacacac gacggttttc gtaccagcag
       61 ggacaaatag gctattcgaa gccaagttta tagacggaga cggcatactg atggcttttt
      121 gtagcgcttt tgcagatatt aactttggag tagaaaataa tgtagttctt ccgtgttccg
      181 catttgtatg caccgaatgc gatgataacc caattgttga gacggtttgc gatggagaga
      241 ggtgtgattt tgaaaatgag actaaagtgt gcgttaacgg cgagtgcata acgcccacac
      301 ctccaccaac cccatcacca acgccaacac cggcaccaac tccaactccg actccaagac
      361 caatactcgg atgctgtgac ttaggacaaa catgtgcgaa cagctcaaat ctccaatgcg
      421 ctgatatgcg aggtttcttc ttccccggcg ctatgtgctt tgcaaatgga tgctcaacga
      481 cgcctccaac acctcctcca accccaactc ctccgccatt tccagtctgc tgtgaatatg
      541 acgatacctg cgctgagaat tcaaacgtag aatgtgatat gttaggaggt acctctttct
      601 tccctgcatc ttgctataat tgtgatggca gttgcatttt cttttgtggt gattaatacg
      661 cccaatcaag tagctcaaca ggatgggcgg ctatttcagt tttacatatt acgtcagaaa
      721 ctattctcat aaagtttatt gaaaatttta aattaaacag acattccctt cggtcattgc
      781 gaggaaccct gagcgaagct ttctttgaac cgcagtgtgg ttcaaaggga aataaaaagc
      841 agcgagggga cgaagcaatc taacacgaag tgtcattccg aaccctgtcc tgagtctaag
      901 cgaaggagaa gtgagcaatc taagatttct cgctatgctc gaaatgacga gaaaaagaat
      961 atttacaatg acacgatgac agtggaaacc ctatagcaag ctacaggtaa ttcttaagtt
     1021 aaaataactg gacttgttgc attttaaata agaaagagtt cttgataaat atccaatatt
     1081 tttaggttgt tgattctata aattaaaatt tatcttgatt ttgaaaaaga ggagttaaaa
     1141 tttactaaag cattccaatc tattttttta ttgcttcatt tagagttgaa gctattgccc
     1201 cataccaatc aatatagtca ccgagacgtt tttcaacatc ctttatctcc tcatctgtaa
     1261 gctctctttc tagctcttgc tccgcgacag tttgtaaatc ttcaatattt atggaatata
     1321 tgaccttact ttttttattc atagtccaaa cctcctattg actcaaggga atatctaatt
     1381 tagttagtga tgcaaaaaac gtttcaaaca tttagattat ctcttaaaga ttcctagtcc
     1441 tcgctccatc tctttgtaat ctcttttctt ccctagtaat tccttcactg atgaaacctc
     1501 aataaatctc atctgttttg ccacttcatg gaaggttcct gaaagtaact cttggtactt
     1561 actcttatcg cccatagcag caacaagaaa aatatggcta ttccaaaggt cgtaagcatg
     1621 ttttagcttt gccattgcat gataaatgtc tcccccgatt tggacctcga acacaaaagt
     1681 aggaactgag cgctcaaccc tacgccaaac tacatcaagg cgtgtacttt caagtggata
     1741 ttcctcatca gcaatgtagc cttggacacg gcctatctct gccaaataag cttttacatc
     1801 atcatgcttt aatgtggtag tttcagttga aatactgaat tcagttggag gcaaagaagt
     1861 agtttcagtt agaagtttta aatccaagga aatccttgca gcattaactt cttcaatagg
     1921 ataacactga aaaaccatcc tacttatcat ctgtaaatta tgtgaagtat atctgtgaga
     1981 tttccataag ctcggtggaa gacaatactc aatatcgaat tcaaagcgga gtggccaaac
     2041 tacttcattt ctctttattt cctccggcca gagaggattc gtttgtctga acttagtgac
     2101 cacatgtcca aagccgacta ttccatgtac tggttttgac acatagaaaa gtaaaccatc
     2161 tccttctcgc agcatgttcc acaaagaccg aagctctcgg aagtctttta atccccaaag
     2221 gtttttgttt tcaaaagcca ctttccagtt cttctctgat ccaactaaaa gccagaattg
     2281 ctgcattatt tgtcacctca ggtagtttcc tcaattcatg ttaactgaag gcttagaaat
     2341 agttccttcc ttgatacgcc caatccaata gctcaacagg atgggcggtt ttaatattta
     2401 atccatgacg tttaataccc atccctattt gcagcagaca tccagggttt cctgcaacta
     2461 aaacctgtac tcctgtttct ttgagcttag agatttttcg ctcaaggagt ttttctgcca
     2521 tatcaggttc tataaggtta taaatccctg cgcttccaca gcagaggtcg gattcgggaa
     2581 gttcaacaaa ctcgattccg ggaatatgtt ttattatttt tcttggatgt tcctttattc
     2641 tctgcgcatg agcaatgtga caggcatcct gatatgtaac ccttaatttc
//
LOCUS       DATDRL010000360         2946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1339964, whole genome shotgun sequence.
ACCESSION   DATDRL010000360 DATDRL010000000
VERSION     DATDRL010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339964"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VI935_11990"
     CDS             complement(<1..196)
                     /locus_tag="VI935_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26348.1"
                     /translation="MACNEMIRKFLNKLYGNAVVFANLRGQSRVPYLPEEKLGELRDA
                     RLKEIVKYAAETVPYYRSLFK"
     gene            complement(239..1834)
                     /locus_tag="VI935_11995"
     CDS             complement(239..1834)
                     /locus_tag="VI935_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26349.1"
                     /translation="MKISRVKQLARITWGLHDYLKSPMTPERAESDIRKRIDNRQGNF
                     LTHAKKLIYENQLSPYRKLLLWAGCEYKDLEDSVTHQGLEKTLEKLRDEGVYITLEEF
                     KTKVPICRKGLTFEISQTDFDNPFLMGKSIQGTTSGSRSGGTRVMYDWDFITEQSANE
                     LMLYETHGLLNVPLALWLPGLPSLSGILNLLMIIKFRRPPEKWFSHLGTGMIGNTVKN
                     RVSIEYLALCCRVFGLSIPRPEFTDIRSATKVAEWMERTKKNKGISVVRTFTSSAVHI
                     VQAATEQGIDISGSVIFTGGEPLTERRHKFIESAGVKVFPRYVATETGLIGASCGNRN
                     YPDDMHLYTDRLAIIQRHRKTLIGGHSVNTYLFTSLSPSTGKVLLNTEIGDFGRLNIK
                     PCSCLFSDLGMNVHVSEVRSYDKLTGEGMTLLGSELDETVGEMIENAGGGPDDYQFWE
                     TQDSRGLNKLIIAISPDIRNLKEKDFIDAILKKLNSKDIGSNIASQIWRQADTLQVVR
                     AYPKLTKGHKMLPIIKEPKQSNL"
     gene            complement(1983..2279)
                     /locus_tag="VI935_12000"
     CDS             complement(1983..2279)
                     /locus_tag="VI935_12000"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HLE26350.1"
                     /translation="MRTNQKILNIQEMGTEDIQKRIKEVLDTVLIPMGSCERHGNPYT
                     PLGLDGLVSGAVVERTARKTEVLHTPLMPFGYAPEHFGRVDEGFRVFEEAAMIV"
     gene            complement(2566..>2946)
                     /locus_tag="VI935_12005"
     CDS             complement(2566..>2946)
                     /locus_tag="VI935_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26351.1"
                     /translation="QRFYLDWPVEWGIHWRAILLEYAKWPYMLLASLNVIMSRRFPYA
                     LTPKVKKKFRNHPLFWPHLVVSIAIGVSSFIGLVSGHVINPFILTCAAIIIITSLALI
                     LTEFLNFPDPYDKKLRNNLNVRHV"
BASE COUNT          774 a          668 c          556 g          948 t
ORIGIN      
        1 ctttaaaaag gctgcggtag taaggcacag tttccgccgc gtatttcacg atctctttta
       61 gcctggcatc acgaagctcg cctaatttct cttctgggag atatggaaca cgactctgtc
      121 ctcgcaagtt ggcaaatacc acagcgtttc cgtagagctt gttgagaaac tttctaatca
      181 tttcattaca cgccattaag tttgttctgc ttctaaggga gccccgactt tcaagatatt
      241 ataagttcga ctgtttaggt tcctttataa ttggtaacat tttgtgtccc tttgtcagct
      301 ttggatatgc tctaacgact tgtaatgtat ctgcttgtct ccatatttga gaggctatat
      361 tactaccaat atctttactg ttcagctttt ttaaaattgc atctatgaaa tccttttcct
      421 ttagattacg tatatctgga ctgatggcta taataagttt atttaaaccc ctactgtctt
      481 gagtttccca gaactgataa tcatccggac ccccgccggc gttttcaatc atttcgccta
      541 ctgtttcatc gagttctgag ccaagcagtg tcattccctc accagttagt ttgtcgtaac
      601 ttcgaacttc tgagacatga acattcatac ctaggtcgct gaataaacag ctacacggtt
      661 taatgtttaa tctgccaaaa tctccaatct cggtattcaa caaaactttc cccgtgcttg
      721 gagaaagaga tgtaaataaa tatgtattga cactatgtcc tccaattagc gttttacgat
      781 gtctttgaat aattgccagc ctgtccgtat aaaggtgcat atcatcgggg taatttcgat
      841 ttccgcagga agcccctatc aaccctgttt cagtagcgac atagcggggg aataccttta
      901 cccctgctga ttcaataaat ttgtgtcttc tttccgtaag aggttcgccg cccgtgaata
      961 tcacactgcc gcttatatct attccttgct cagttgcggc ttgaacaatg tgaacggctg
     1021 aactcgtaaa agtccttacc acgctgattc ctttgttttt tttagtcctt tccatccatt
     1081 ctgcaacctt agttgcacta cggatatcag tgaactcagg tcgcggtata gaaagaccaa
     1141 acacgcgaca acacaatgct aagtactcga tagagacgcg atttttaaca gtgtttccaa
     1201 tcattccagt gcccaagtga gaaaaccact tctcaggcgg cctgcgaaat ttaataatca
     1261 tcagaaggtt aagaatccca gataagctcg gtaatccggg taaccacaaa gctaaaggaa
     1321 cgtttaacag tccgtgagtc tcgtagagca tcaattcatt ggcgctttgc tctgtaatga
     1381 aatcccaatc atacataact cgtgtaccac ctgaacgact tccacttgtt gttccttgaa
     1441 tgcttttacc cataaggaag ggattgtcaa aatcggtctg gctaatttca aaagtcaatc
     1501 cttttcggca aatcggaacc tttgttttaa attcctcaag tgttatgtaa accccttcgt
     1561 ctcgtagttt ttctaatgtc ttctctagcc cttgatgcgt gacgctatcc tcaaggtctt
     1621 tgtattcgca tccggcccat agaagaagct tgcgataagg gctgagttga ttttcataga
     1681 taagtttctt cgcatgagtt aagaaattgc cctggcgatt gtctatccgc ttcctaatgt
     1741 cactttctgc ccgttcggga gtcattggac ttttaagata atcgtggagc ccccaggtaa
     1801 tacgggctag ctgtttgact cggcttattt tcatatcgga gatctttatt aagaaaagtt
     1861 aaaccactga aatctgtttg aggtttatca atataaccta gcttcataac aagagagaaa
     1921 cccttccaag ccctcttatt ttgaatataa actcttctcg tccgtaaggg atttttttct
     1981 tttcaaacaa tcatagcggc ttcttcaaaa accctgaacc cttcgtctac tcgaccaaag
     2041 tgctctggag cgtaaccaaa aggcatcaaa ggcgtgtgca ggacttcggt ctttctcgcg
     2101 gttcgctcga cgaccgcgcc actcacaagg ccgtcaagcc ccaagggagt atatgggttc
     2161 ccgtgccgct cacagctccc cattgggata aggacggtat caagcacttc cttaatccgc
     2221 ttctgtatat cttccgttcc catctcttgg atgtttagga ttttctggtt tgttctcatc
     2281 gaattctcct accttcttcc tcggcaattt gcatcatgtt tagtttttta aggctttaaa
     2341 tatttcctgt aagtttctca ttttcatttt acgctattta gttaggattt ctgagagttt
     2401 tctcgaaact ctaaatttga taattttgtc tggtttcatt ttgttattac cgcttaagat
     2461 aacgttaaat gaataaaatc gtgtcttact ccgatggatc atggatccat ttcagtcatg
     2521 cttgctttct acagagacta taagctctcc atagagctcc aagtcttaca catgtctcac
     2581 attcaaattg tttcggagtt ttttatcgta tggatcaggg aaatttaaaa actccgttaa
     2641 gattaaagca agtgaagtta taataattat tgcagcacat gtaagaatga atgggtttat
     2701 aacgtgccca gagaccaaac cgatgaacga agaaactccg atggcgatac taacgaccaa
     2761 gtgtggccaa aacaaggggt ggtttctgaa ttttttcttc acttttgggg ttaacgcata
     2821 cgggaatcgg cggctcatta taacattaag ggatgctaaa agcatatacg gccattttgc
     2881 atactctaac aatattgctc tccaatggat tccccattct acaggccaat ctagataaaa
     2941 tcgctg
//
LOCUS       DATDRL010000361         2826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2008878, whole genome shotgun sequence.
ACCESSION   DATDRL010000361 DATDRL010000000
VERSION     DATDRL010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2008878"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VI935_12010"
     CDS             <1..845
                     /locus_tag="VI935_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HLE26352.1"
                     /translation="NNIWQMGRLSKRMPQTTILFLIGALALSGIFPFSGHFSKDEILV
                     WMYVKGFRIHFFILLFTAFLTALYMFRTFFIVFMGDKEAREHPHESPWVMTLPMWILG
                     LLALIAGFPKESIFKFLGHDSESYHSYIAYLPLSFSILEIMTAWSFYQMKFFKPSNIA
                     EALSPITSALEKKLWLDDIYEWFYRGILDGLSYLCGWFDRYIVDGIVNATSWYIKQAS
                     SQLREIQTGRIQDYLYGIIAALLLLIWLTLGVSPVKDRINEEIVKPKDHIQAPSQYLE
                     GEKQ"
     gene            848..2401
                     /locus_tag="VI935_12015"
     CDS             848..2401
                     /locus_tag="VI935_12015"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HLE26353.1"
                     /translation="MTSETLPILSLITLSPFIGAVILLLQPERWYLPIRLTALSFSII
                     SFLGSIIAVLYYDSELGGFQFNEYIPIVHSLGIGYHLGADGISLVLILLTGFILVTGV
                     FASWTIPFRSKEFYILLLLLVTGVFGVFVSLDLFLLFLFYELAVLPMYLLIGIWGSST
                     KIAAKGPFARAIMDMGLGSKEYGAMKLTLYLLFGSAFILVAILGLYSLSGLKTFDYIA
                     LEGVVLPSSIQNWLFLLLYVGFGSLAGIWPIHTWSPDGHAAAPTATSMMHAGVLMKLG
                     AYGIIRIGMGLLPEAAQTLAPIIGTIAVINIVYGAFGAMWQRDLKYVVAYSSVSHMGI
                     VMLGAATLNEMGLNGSVLQMFSHGIMTGLLFALVGLIYEKAHSREILQMGGFAKRMPG
                     IATFFAIGALTSLGLPGLSGFVAELLVFLGAWKSPYFWWLIPAVFGAFITAVYALRVL
                     KWIFFGTDKEPEINHKGNPNGLKDAIGVEWIAPIILSAVLIVVGIYPKPLLQLMDSSV
                     IDFLERVLK"
     gene            2426..>2826
                     /locus_tag="VI935_12020"
     CDS             2426..>2826
                     /locus_tag="VI935_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HLE26354.1"
                     /translation="MFKLLTLEIALIIGIFVIFLLDLFLQRERKRVLGFLTFLLLIIV
                     LGISMSKSIYGTAFSGTFVSDSFTVTLNQIFLIATGLAVLGSISHTEKKLKGREGEYY
                     LLLLFSLLGMMITVSSRELLLLLVGFELMSIP"
BASE COUNT          720 a          595 c          569 g          942 t
ORIGIN      
        1 ctaataatat ttggcaaatg ggaaggctct caaagaggat gcctcagact accatactct
       61 ttcttatagg tgcattagct ctttctggaa tatttccctt ctccggtcat ttttcaaaag
      121 acgagatttt agtctggatg tatgttaagg gattcagaat ccactttttt atccttcttt
      181 tcaccgcctt tctcaccgcc ctttatatgt ttcgtacatt ttttatcgta tttatgggag
      241 ataaagaagc cagagagcat ccgcacgaat ctccttgggt aatgactctc ccaatgtgga
      301 ttcttggact acttgctcta attgccggat ttccaaaaga aagcatattt aaattcctgg
      361 gacatgattc agaatcttat cattcctata tagcatacct tcccctatca ttctcgattc
      421 ttgaaattat gacagcgtgg tcattttacc aaatgaaatt ctttaaacca tccaatatag
      481 cagaagccct ctcacccatc acatcagccc tggagaagaa actctggctc gatgacattt
      541 acgaatggtt ctacagaggt attcttgatg gtctttcata tctttgtggc tggtttgata
      601 ggtacattgt tgacggaata gtaaacgcta cttcttggta tatcaaacag gcttctagtc
      661 agctaagaga aattcaaaca ggacgcatcc aggattacct ttatggaata atcgcagcgc
      721 ttcttctcct tatctggctt acactaggag tatcccctgt taaagaccga ataaacgagg
      781 agatagtaaa acccaaagat cacatccagg cgccttctca atatcttgaa ggagaaaaac
      841 aataaacatg acgagtgaaa ccttacctat tctttcttta attacacttt ccccgtttat
      901 cggagctgta attcttcttc ttcaacctga aagatggtat cttcccataa gacttactgc
      961 cctcagtttt tcaattatat cgttccttgg aagcataatt gcagttttat actatgattc
     1021 tgagcttggt ggatttcagt ttaacgaata catcccgatt gttcatagtc taggaatcgg
     1081 ttatcactta ggggcggatg gaataagcct cgtactaatt ctgcttactg gttttattct
     1141 agttacaggg gtttttgcat catggacaat accctttaga agcaaagaat tttatatctt
     1201 gctccttctt ttagttacag gtgtgtttgg ggtttttgtt tcccttgacc ttttcctgct
     1261 ctttttgttc tacgaactcg cagttctccc tatgtatctt ttaataggga tatggggctc
     1321 ttcaaccaag atagcagcaa agggtccatt tgcaagagca attatggata tgggtcttgg
     1381 gagtaaagaa tacggggcta tgaagctcac cctttacctg ctttttggct ccgcttttat
     1441 tctagttgcg attctaggac tctatagcct ctcaggactc aaaacatttg attatatagc
     1501 acttgaagga gtcgtacttc cttcgtctat tcaaaattgg ctttttctcc ttttgtatgt
     1561 cggttttgga agccttgcag gtatttggcc tatccacacc tggtccccag acggtcatgc
     1621 agcagcaccc actgctactt caatgatgca tgccggagta cttatgaagc ttggcgccta
     1681 tgggatcatt cgcattggta tgggacttct tcctgaggca gcacaaacac ttgcccctat
     1741 tatcggaact attgctgtaa taaatatcgt atacggagcc ttcggagcga tgtggcagag
     1801 ggatttgaag tatgtagttg catattcatc tgtttcacac atgggaatcg tgatgcttgg
     1861 cgccgcaacg cttaacgaaa tgggtcttaa tgggtcggtt ctacagatgt tttcacacgg
     1921 tattatgacc ggactccttt ttgcactagt cgggcttatc tacgaaaagg cccacagccg
     1981 cgaaattctc caaatgggtg ggttcgcaaa gcgtatgcct ggcatagcta ccttctttgc
     2041 tattggcgct ctcacttcct tagggcttcc tggtcttagt ggttttgtcg cagaattgct
     2101 tgtttttcta ggagcttgga aatctccata tttctggtgg ttgatccctg cagtctttgg
     2161 tgcttttatc acagcagttt atgcgcttcg ggttcttaaa tggatctttt tcggtactga
     2221 caaagaaccc gagattaacc ataagggaaa tcctaatggt cttaaggacg caattggcgt
     2281 tgagtggatt gcccctataa ttctttcagc ggttctaatt gtggtaggaa tctatccgaa
     2341 accgctatta caactgatgg atagctctgt aatagatttc ttagagaggg ttctaaagta
     2401 agaatgctta ggggaaacat agcccatgtt taaactgctc actttagaaa tagcacttat
     2461 tataggaata ttcgtaatct tccttttaga cctttttctc caaagagaga gaaagcgggt
     2521 cttgggtttc cttacgtttc ttttattaat tattgtttta ggaataagta tgtcgaagtc
     2581 aatttatgga acggcctttt caggtacctt tgtgagcgat tcctttacag tcactctgaa
     2641 ccaaattttc cttatagcaa caggactagc cgttttaggc tctataagcc atacagaaaa
     2701 aaaacttaag ggacgagaag gcgaatacta tcttcttctt cttttctcac tccttggcat
     2761 gatgataact gtttcttcaa gagagttact ccttctctta gtcggattcg aacttatgag
     2821 tattcc
//
LOCUS       DATDRL010000362         5960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_692547, whole genome shotgun sequence.
ACCESSION   DATDRL010000362 DATDRL010000000
VERSION     DATDRL010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692547"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VI935_12025"
     CDS             complement(<1..197)
                     /locus_tag="VI935_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26355.1"
                     /translation="MPETPTLRTGNLGLGILLTVFAFLSVAIMSALGKAASPSVSSGV
                     LVFFQNSISLVLLLPWVIRDG"
     gene            complement(321..1427)
                     /locus_tag="VI935_12030"
     CDS             complement(321..1427)
                     /locus_tag="VI935_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLE26356.1"
                     /translation="MKAITIIPETTTVRLQDREEPSISATDEIKIQMRRVGICGTDRE
                     EVAGGRALAPQGQKELIIGHEMFGQVVEVGKDVTSVKPGDYAVFTDRRGCGECIPCGM
                     NRSDMCNTGKFRERGIWGLDGFQTEYVVDKEQYVVRVPAELEPVGVLTEPLSVAEKAI
                     DVALRVQIARLPDAQTKPDWLFERRCLVAGLGPIGLLAAVALRLRGAEVYGLDIVEPN
                     STRPLWLEHIGGKYIDSHQMPVDRIDQAIGSMDLIFEATGVPKLQFNILDALAYNGVY
                     VVTGIPGGDRPLEIPGAELMRQLVLKNQLLLGSVNNPRAYFQMAVDDLSIAQLKWGDH
                     IKKLITNRYPYTEFVKPLTQHEADEIKVVLEWKV"
     gene            complement(1842..2231)
                     /locus_tag="VI935_12035"
     CDS             complement(1842..2231)
                     /locus_tag="VI935_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015162911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HLE26357.1"
                     /translation="MSRTSFRLTDQQVETSIGNLLRISVIIAAFVVLLGGIIYLVRFM
                     GAHPEYRVFTGEPRDLRTVSGIFMDALEIHSRGIIQLGLLILIATPVARVILALFAFA
                     LQRDRIYVVVSLIVLTVLIYSLSGGRP"
     gene            complement(2232..3068)
                     /locus_tag="VI935_12040"
     CDS             complement(2232..3068)
                     /locus_tag="VI935_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015162912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLE26358.1"
                     /translation="MNVLEFSILIWLASLLAGFLGALTGLGGGVVIVPLLALGFGVDI
                     RYAIGASLISVIATSSAAAAAYVKEGFSNIRIGMFLEIATTTGAVVGASIASRMPTST
                     IAIIFGVVLLISAYLSSVTRRERTGQSEPDPLATHLRMNASYPTPQGQEQYQVHAVPA
                     GFSLMFIAGALSGLLGIGSGALKVIAMDQVMRIPFKVSTTTSNFMIGVTAAASAGVYL
                     NRGYVDPGLAMPVMLGVLIGSLMGTRLLVTAKTRILRIIFALVIFVMALEMLYNGITG
                     RL"
     gene            3545..3787
                     /locus_tag="VI935_12045"
     CDS             3545..3787
                     /locus_tag="VI935_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26359.1"
                     /translation="MHDDDFEIIGEITNIETIAVGNAIRDIKRLHKSYGTDRWRKLKG
                     IAKIRLTSRRIRTAEVHWYEAHGIGKKEMKRKRYLD"
     gene            3792..4046
                     /locus_tag="VI935_12050"
     CDS             3792..4046
                     /locus_tag="VI935_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002751846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26360.1"
                     /translation="MKKPKNTTQLFAVCVDNSGYPASLELFKIYRVLPDDDAAVDGDL
                     RVVDESGEDYLYTADRFVLIDLPQVLIKRIINKTVADETD"
     gene            complement(4419..4823)
                     /locus_tag="VI935_12055"
     CDS             complement(4419..4823)
                     /locus_tag="VI935_12055"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HLE26361.1"
                     /translation="MTLSSRLRYKSLYEAQVPTRDESPAHYLGEIQNALPLLGEHRFS
                     LSSTVGHLELQELLTSGIAIDLSGGKQLTIGVLRSDSPCVSKPVKSDCLNLGDEDVHM
                     VAILRENKVLLPHPEMVLQSGHRLMMITSPNA"
     gene            complement(5194..5811)
                     /locus_tag="VI935_12060"
     CDS             complement(5194..5811)
                     /locus_tag="VI935_12060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLE26362.1"
                     /translation="MSDKLSFILNPVINKLNAISPAFIKRLFDLFWIKTARFQRNMKS
                     GEISIRLYSLKDLYTLYSLFNQGNLINFDTTRYGVFNYLSHYFRLMFDKFHVFYLIKA
                     REKDVSRIIGFMGLYNIVIGQTVFLSIAIFKPDDRRQGYGKGAIELLLNNLKKNNIVK
                     SIHVEVLTTNEPSLCFFKGLGFEICRQYNDRFLLEKNLENTQQKS"
BASE COUNT         1833 a         1313 c         1174 g         1640 t
ORIGIN      
        1 aatccatctc ttatcaccca tggcaataga agaacaaggc ttatcgaatt ctgaaaaaag
       61 acaaggacac cagatgaaac cgatggagaa gccgccttcc ctaatgcgct catgatagcg
      121 acagaaagga aggcaaaaac agtaagaagg atgccaagcc caagattgcc agttcttagc
      181 gtgggtgttt caggcataaa tccaagaccc ctcctggatg atgatatctg taaaccaaat
      241 aagttttgat gtttctgtca ttttaatctt ggcagaccac tactattacc tattctggtc
      301 gccgcgtgca ataactaagc ttaaaccttc cactccaaca ccaccttgat ttcatcagct
      361 tcatgttgag taaggggctt aacaaactca gtgtagggat agcggttggt tataagcttt
      421 ttaatgtgat caccccattt caattgcgca attgataggt catccacagc catctgaaaa
      481 tatgcacgag ggttattaac gcttccaagg agaagctgat tctttaggac caactgacgc
      541 ataagttccg cgcctggaat ctctaacgga cgatctccac ccggaatgcc tgtgactacg
      601 tataccccgt tataagccaa ggcatcgagt atgttgaatt gaagtttcgg aaccccagtg
      661 gcttcaaata taaggtccat agaaccgatc gcttggtcaa tacggtctac cggcatttga
      721 tggctatcta tgtacttacc gcctatgtgc tctaaccata ggggacgagt agagttaggt
      781 tcaactatgt ccaagccgta tacctcagcg ccccgaaggc gaagcgccac tgcagccaag
      841 agaccgatag gtcctagtcc ggccacaagg caacgccttt cgaagagcca atcaggttta
      901 gtctgtgcat ccggaagtcg tgcaatctga actcggagag ccacatctat agccttttct
      961 gctacggaaa ggggctcagt tagcacccca accggctcca gttcagcagg cacacgcacc
     1021 acatactgtt ccttatctac aacgtattcg gtttgaaacc cgtccagtcc ccatattccg
     1081 cgttcgcgga atttacctgt attgcacata tcagaacgat tcatgccgca aggaatgcat
     1141 tcaccacaac cacgacgatc tgtaaacaca gcataatctc ccggcttaac gcttgttaca
     1201 tccttaccaa cttcaactac ttgtccaaac atttcgtggc caatgatgag ctctttttga
     1261 ccttgaggag caagagccct tcctccagcg acctcttccc tatctgtgcc acatattcca
     1321 acacgtctca tttgaatctt gatctcatca gtggcactga tggatggctc ttcacggtct
     1381 tgaagtcgaa ctgtggtcgt ttccgggatt atagttattg ctttcatcta tttctcctaa
     1441 acatcagata ttttatctaa ttcatagaca ccttaactgc ttaacctgct aattaacgtg
     1501 agttcaatag cttttcaaga gcaatgtagc gaaggccata tcgtttctaa aaatttaatc
     1561 aggcaacaaa tgtcctggta acaaaccgcc cgggttgacc accagttcct gccctgagcg
     1621 aagtcgaagg gttgttggtg gtctaggtgg caacgagcaa gcctgtcctg agttataacg
     1681 aaggaactcg ttgcctaacc acttatgtta tttattactt gattaatgat taaatgacca
     1741 ttagacctcc ataaatgtaa tcctaaagga ttccgctaca attattctct tacgttaatt
     1801 cagtttgatt ataattctgt gtaagaaact gactgtcttt atcacggacg accacctgaa
     1861 agactataga tgagcacagt gagcacgatt aaacttacaa cgacatatat acgatcgcgt
     1921 tgtaatgcaa aggcaaaaag agccaatata acacgtgcca caggtgttgc aattaaaata
     1981 aggagtccta gctgaataat tccacgactg tgtatttcca aggcgtccat aaaaattcct
     2041 gaaaccgtgc gcaagtcccg gggctctcct gtaaaaactc gatattcagg atgcgccccc
     2101 atgaaacgta caaggtatat gatccctcct agcaaaacga caaaggcagc aattattacc
     2161 gatattctca acaaattccc tatgcttgtc tcgacctgct gatctgtcaa cctaaagcta
     2221 gttctagaca tttaaagcct cccggttatg ccattataga gcatctctag agccattaca
     2281 aagataacaa gggcgaatat aattcttaat atccgtgtct tagcagttac cagtaaacgt
     2341 gtccccatca atgaaccaat caaaacgcca agcatgacgg gcattgcaag cccaggatca
     2401 acatagccgc ggtttaggta aacaccggca ctagctgcag ccgtcacgcc aatcatgaag
     2461 ttgcttgttg ttgttgaaac cttaaatggt attcgcataa cctgatccat tgcgattacc
     2521 ttgagggcgc ctgatccaat tccaagcaag ccagaaagtg cacccgcaat gaacattagg
     2581 ctaaatcctg cgggcacggc gtgtacctgg tattgctctt gtccttgagg tgtagggtag
     2641 cttgcgttca tccttagatg tgtggctagt ggatccggct cactctgccc tgttcgctca
     2701 cgtctagtaa cactcgatag ataagccgag atgagaagaa ccaccccaaa gattatcgca
     2761 attgtactag taggcatcct agaggctata gaagccccga caacagctcc agttgtagtt
     2821 gctatttcta gaaacattcc tatgcgaata tttgagaaac cctctttgac ataggctgct
     2881 gccgccgccg aagaggttgc aataacagag ataagcgagg ccccaatggc atacctaata
     2941 tcaactccaa agccgagagc cagtaatgga acaatcacaa cgccgcctcc gagccccgtc
     3001 aacgcaccca aaaagcctgc taacaaagat gccaaccaaa taagtatgga aaattcaagc
     3061 acattcatcg gaatagttat cctaacatta gcattgtatt attccaatta ttaaggtcaa
     3121 aagattccaa tgctatagtt ccagaggttt atagtaggag caccgttccc ggtgcgacat
     3181 aattatcgca gcaagatgcc gctcctacgg ttgattggga actccatcat aatctgtagg
     3241 acagacttcc cagcctaaat tattgaaccg agattggata acctcgacta tattttgggg
     3301 ggtattttat gaagaaaccg ttcaaactga gaaaacactg taggcacgaa cttgtttgtg
     3361 ccaatgatag cacaggcaag tctgtgccta caatatttga acagttatca attagaaacg
     3421 gtattatagt tcactgagat tgcattattg ctattatgtt gttatgttgt atattcaaaa
     3481 ttattagtca aaaagcttct cgttgcgttg ctgcttgaat tgattatagt cttacatgtt
     3541 ttagatgcac gatgatgatt ttgaaataat tggcgagatc acaaacatcg aaacgattgc
     3601 ggtaggtaat gcaatacgag atataaagcg cctgcataag tcctacggca cagacaggtg
     3661 gcggaaactt aaaggaattg ctaaaattcg tcttacctct cgcaggatac gcactgccga
     3721 agtacattgg tatgaagccc atggcatagg caagaaagaa atgaaacgta aacgttattt
     3781 ggattagtta aatgaagaaa cctaaaaaca ctacacagct atttgccgtt tgtgttgata
     3841 actctggtta cccagcttca ttggagttat tcaagattta tcgagtgctg cccgatgatg
     3901 acgccgcagt agacggggac ttacgagtag ttgatgaaag tggggaagac tacttatata
     3961 ctgctgaccg tttcgttttg attgatttgc cacaggtatt aatcaagcga attataaaca
     4021 aaacagttgc agacgaaacc gattgaaaaa aaacaataga ggtcgcagcg ctgagccgcg
     4081 ctagcgacct agcaacacta aaattttttt atatcttgta ccatttccaa taaggaaatg
     4141 accgatgggc attgtaggca cgaacttgtt cgtgccaatg atagcacaga caagtctgtg
     4201 cctacataat tgaacagtta tcacatggaa gtggtataaa attataatac atagaatgaa
     4261 agcgactgca gatttctaaa tgtagggctc gttccccgaa cgagcctccg atgaattcat
     4321 cgggggtggc tgatgaggac ataagcccct acttaatctt cttcatgatg cctctttctc
     4381 taagacgata gcgaggagag gtgctttgat agccgtcctc aagcattggg tgaagtaatc
     4441 atcatcaatc gatggcctga ctgcaagacc atctctggat gaggcaaaag caccttattc
     4501 tctcttaaga tggcaaccat atgcacatcc tcatcgccta aattaagaca gtccgatttt
     4561 accggcttac taacacaagg actatctgac ctcagcaccc caatggttaa ctgcttgcca
     4621 cctgagaggt caattgcaat tccagaagtc aaaagctcct gaagttcaag atgtccaact
     4681 gtactggata aagaaaaacg atgctcacca agtaatggta aagcgttttg tatctctcct
     4741 aaataatggg ctggtgactc atccctggtc ggtacttgag cctcatagag acttttatac
     4801 ctaagacgac tagataaagt cacctggaag aaataactga tcataataga taaagcagca
     4861 gggtatggtc ttaaaaatat accgttatag ctgtcattgc gaggaggaaa acgacgaagc
     4921 aatctaaaat gagagattgc ttcacggagc ctgcactgaa cgaaatgaac gtgttcgcaa
     4981 tgacaataca ctattgtgaa aaccctgtag caagctacag ggaattctca agttaaattt
     5041 gctaaggttg taatcttatg attaattgtc gaatatttct atgccgcctt cataaatcca
     5101 acattaccgt agaccttata agctcaattt aatacaacgt ttttaacctg aattaacaga
     5161 ataactatac gagaaggtca agtttattcg aaatcaagat ttctgttgtg tattttcaag
     5221 attcttctcc aacaaaaacc tgtcattata ttgcctgcaa atttcaaaac ctaatccttt
     5281 aaaaaagcat aatgaaggct cattggttgt taatacttca acatggattg attttacaat
     5341 attgttcttt ttaagattat tcaggaggag ttcgatagcc cctttcccat acccctgtcg
     5401 tcgatcgtcc ggcttaaaaa ttgcgatcga taagaaaaca gtttggccaa ttacaatatt
     5461 gtagagaccc atgaacccta ttattcggct aacatccttt tccctagcct tgattaaata
     5521 aaaaacatga aatttatcaa acatcaatct aaaataatgt gagaggtagt taaatacacc
     5581 atatcgtgtt gtatcaaaat taataagatt tccctgatta aacaatgaat ataaagtata
     5641 tagatccttc aagctataga ggcgtattga aatttcaccg ctcttcatat tacgctgaaa
     5701 tcttgcagtt ttgatccaaa ataaatcaaa aagccttttg ataaatgctg gacttattgc
     5761 atttaatttg ttgattacag gattgagaat aaatgataac ttgtcactca tgatgctaga
     5821 catgtattaa atcatttttt gtacattgtc tcaaatgaca ttgtacatta ctcaatatct
     5881 ctgctatttg aaatttaaag tatttagtgt tatcacttga catatataca ttataatttt
     5941 tataattgat gcgatttaaa
//
LOCUS       DATDRL010000363         3348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2270061, whole genome shotgun sequence.
ACCESSION   DATDRL010000363 DATDRL010000000
VERSION     DATDRL010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2270061"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VI935_12065"
     CDS             <1..350
                     /locus_tag="VI935_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26363.1"
                     /translation="KRPHEALEMRVPAQRYRRSTRVYQGGINKKTQIKYPEGYKVRRV
                     RRSGEIRWKGKTRFVGAALKGVKVGIKQVEEGHNQVYFDYILLGDLYDIDYTGLRPMV
                     GVHQEDEKKGEKV"
     gene            complement(446..616)
                     /locus_tag="VI935_12070"
     CDS             complement(446..616)
                     /locus_tag="VI935_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26364.1"
                     /translation="MLQGVVIDDSKLFKERLRECGNFYNFQRPHGFLSGQTPFERLKE
                     KLKEFSLTEVTL"
     gene            934..1128
                     /locus_tag="VI935_12075"
     CDS             934..1128
                     /locus_tag="VI935_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLE26365.1"
                     /translation="MIPEDHICFLVDSKVESMDFASYDIRYSGAEHPAYHLRILLKIL
                     IMGILNRVRSSRRLAKNTLR"
     gene            1461..1640
                     /locus_tag="VI935_12080"
     CDS             1461..1640
                     /locus_tag="VI935_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26366.1"
                     /translation="MKVSKKYPFVLARLLIPVKNLRVTNNLKGNRLVIDDVLNIPKSE
                     KEAAKKVGSRMQYNK"
     gene            1687..2148
                     /locus_tag="VI935_12085"
     CDS             1687..2148
                     /locus_tag="VI935_12085"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HLE26367.1"
                     /translation="MSDCNFLNTCMKKESYAVDTVVRHEKTVNSSLAEKDIETFLPIR
                     DVVSHWKDRRKRVKFSFFPGCLFVNMILQDRWSVLNTRGVVRILGVYGNTTPVPFDQI
                     DAIIREQAGIRSLSFFTQGREIVVINGPPQPEVGPKKPPQGGKKCILTLIT"
     gene            2124..>3348
                     /locus_tag="VI935_12090"
     CDS             2124..>3348
                     /locus_tag="VI935_12090"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HLE26368.1"
                     /translation="MHTYFNYLKEQVSNSRITKCLLILYATILFHPISVLAEVITLTP
                     VKDTYINQKNSGSSYGSSTLLQTKFREGSKREALLQFDLFGIPQGAQITTAYLKLYSD
                     YKRQFRKDTATRVYATLGSWGESTTWNTKPPRASSYEDSIAVTSTRQYIQWNITNLVQ
                     DWVDGEETNYGVTIRSEGGGELYFTSRDSSSNKPILYIEYSILTLSPTLHVTSYGAKG
                     DGTTDDTAAFIQVLAIAANRTVIIQPGTFRITSNITVNSNVTLYFQNGAKLLPDFVTI
                     TINGPIIAGLYQIFTGSGDIRLNSFAANMVVPQWFGAVGDGVAIDTVALRKWAASIQT
                     GGGDLFLPEGTYMYNSYVPIPSNTRLTCADADSAVFKNNGTAQGLFNFASPATSDVTV
                     ENCGFDANGYNVEDFQ"
BASE COUNT         1067 a          597 c          747 g          937 t
ORIGIN      
        1 agaagcggcc gcacgaagca cttgagatga gagtgcctgc tcaaaggtat cgtcgttcca
       61 caagagtgta tcaaggaggg ataaataaga aaacacagat caagtatcct gagggatata
      121 aagtaagacg agtaagaagg agtggagaga ttagatggaa aggaaagacg agatttgtag
      181 gagcagcttt aaaaggtgta aaggtaggaa taaaacaggt agaagagggt cataaccagg
      241 tgtattttga ttacatactc ttgggagatc tatatgacat tgattataca ggactacgac
      301 ccatggtcgg agttcaccaa gaagatgaaa agaagggaga aaaagtgtaa cctgtgttcc
      361 cggtttaaag tgtaacctat atccccgttc gcacgagttt aaacggagac ctgggtaact
      421 gtacgttagt aagacaccgg taacatcata gagtaacctc ggttaaagaa aattctttca
      481 gtttttcttt caacctttca aaaggggttt gaccagaaag aaacccgtgt ggcctttgaa
      541 agttatagaa gtttccacat tctcgaagcc tttctttaaa gagctttgaa tcatcaatga
      601 caaccccttg aagcattcga tagaattcct cctcatcgat tcgatcagat cgttctactt
      661 ttcctgagtc ttggtgtatg aggtttaatc tatctatgtt caatcccttt agctagagtg
      721 tgtcactgga attgtggtgc aaactcagct ccattgtcag tttggattga gcttgagaat
      781 tccctgtagc ttacaggctg tccgataatt aggatagata gattataata actgctgtca
      841 aggaaaaaaa tcttcagaga ggaggaatta aagctggcat acattgaatc atataaagga
      901 cagcagtggt tgttaccgcc gagcctggag gagatgatac cagaagacca catatgtttt
      961 ttggtagata gcaaagtaga gtcaatggat tttgcttcct atgatataag gtattcaggg
     1021 gccgaacatc cggcatatca tctcaggata ctacttaaga tattgatcat gggaatactg
     1081 aacagggtac ggtcatcgag gaggctggca aagaataccc ttagataatt atcagacagc
     1141 ctgcttaccg cagggagctt cattgaacag aggatggaag tttagctaga tcataatcca
     1201 caaattcaat agaattcttt tgattgaacc tattgtaaat attaagaatc ctgtacttta
     1261 tggagttcgg gtaggattgg tgaagctcga ccaatagcgc aatagcagac gagccagccg
     1321 agaagccagg tcttttttcg gtccatctca ggttttttcc ctatgtttct caggtttttt
     1381 accgatagta gcttagatta atttagatta atatcaagaa ctagaggtta aaaattaatt
     1441 taacaaggag gtttagaaaa atgaaagtat caaaaaaata tccattcgtt ttagcgcgtc
     1501 ttttaatacc ggttaaaaac ctaagagtta caaataatct caagggtaac cgtttggtta
     1561 tagatgatgt tttgaatatc cctaagtctg aaaaagaagc tgcgaagaag gtcggctcta
     1621 gaatgcagta caataagtga tcgattaaag agttaaatta caagattgaa gataataggg
     1681 tagaagatga gtgattgtaa ttttcttaat acctgtatga agaaggaatc gtatgccgtt
     1741 gatacggtag ttagacacga aaagacagta aattcatctc ttgctgaaaa agacatagag
     1801 acgtttcttc caattagaga tgtggtaagt cattggaaag atagaagaaa aagagtcaaa
     1861 ttctcttttt tcccaggatg cttgtttgtt aatatgatcc tccaagatag atggagtgtt
     1921 ttaaatacac gaggcgttgt taggattctt ggcgtatatg gaaataccac tccagttcca
     1981 ttcgaccaga ttgatgcaat aattagagag caggcaggaa tacggtcctt atcctttttt
     2041 actcaaggaa gagagattgt tgtcataaat ggaccccctc agccggaggt tgggccaaag
     2101 aaaccaccac aaggaggtaa aaaatgcata cttactttaa ttacttgaaa gagcaagtct
     2161 ctaatagcag aatcactaaa tgtcttttga ttctatatgc aacgattctg tttcatccga
     2221 tctcggtact ggctgaagtg attactttaa ctccggtaaa agacacatat ataaatcaaa
     2281 agaactcggg gagttcgtat ggaagcagca ctttattgca aacaaaattt agagagggta
     2341 gtaagagaga agcgttatta caatttgatt tattcggtat cccccaaggc gctcagatta
     2401 caacagcgta ccttaagctt tacagtgact ataaacgaca atttcgtaaa gacaccgcca
     2461 ctagggttta tgcaaccttg ggttcctggg gcgaatccac aacctggaat acaaaaccgc
     2521 ctcgagcttc atcgtatgaa gatagcattg ctgtaacaag tacacgtcaa tacattcaat
     2581 ggaatataac aaatctggtt caggattggg ttgacggaga agagacaaat tatggagtaa
     2641 cgattcgttc ggaaggaggt ggtgagttat actttactag tcgtgattct tcgtcaaata
     2701 agcctatttt gtacattgag tattccattc ttaccctttc accaactctt catgtaacaa
     2761 gctatggtgc aaaaggagat ggcacaacgg acgataccgc tgcttttatt caggtacttg
     2821 caattgctgc aaatcgcacc gtcattatac aacctggcac ttttcggata acatcgaata
     2881 taacagttaa ctcgaatgtc acattgtact ttcaaaatgg agcaaagctt ttacctgatt
     2941 ttgtgactat caccatcaat ggtccaatta tagcaggctt ataccaaatt ttcaccggaa
     3001 gtggagatat ccgcttgaac agtttcgctg caaatatggt ggtgcctcag tggttcggag
     3061 cagtaggaga tggagtagca atcgacacag tggcactacg gaagtgggca gcctctattc
     3121 aaacgggagg gggcgatctc tttctgccag aagggacata tatgtataat agctatgtac
     3181 ctataccttc aaatacccgt ctaacctgtg ctgatgctga ttctgccgtg tttaaaaata
     3241 acggcacggc gcagggactc tttaactttg caagtcctgc tacttccgat gtaaccgtcg
     3301 agaactgtgg attcgatgca aatggttata acgtagaaga ttttcaat
//
LOCUS       DATDRL010000364         2059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3060732, whole genome shotgun sequence.
ACCESSION   DATDRL010000364 DATDRL010000000
VERSION     DATDRL010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3060732"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VI935_12095"
     CDS             <1..259
                     /locus_tag="VI935_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26369.1"
                     /translation="DELKERLESLSQKFKDFRQTKLGKTIYWLASNSLGFIPWIGTPA
                     SLGLSFYDEFLVKDFLPKGGAMTFINNKLPTVYKETQEVKP"
     gene            295..726
                     /locus_tag="VI935_12100"
     CDS             295..726
                     /locus_tag="VI935_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HLE26370.1"
                     /translation="MITVPKLTLDDAKIIVDGAEKKAKEIGVDMDIAVVDDGGNLLAF
                     YRMDKAKITSIDVAINKAFTAAGARKATHEYAEIAGAGGPAFGIHVSNRGRFMIFGGG
                     LPIFVDGHIVGGVGCSSGTVEEDRIVAQAGIDTLMKHLKKY"
     gene            complement(768..1166)
                     /locus_tag="VI935_12105"
     CDS             complement(768..1166)
                     /locus_tag="VI935_12105"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLE26371.1"
                     /translation="MDKVVLDAFAILTLLGKENGYEIVKDYLERSYSGKVRAYISLIN
                     WGEVYYTLIRRGKTGDAEEFWEERRSFPIVFLEPTARRIRSASKVKATYPVSYADAFC
                     IALAMELEAKVITGDEEFKAVNGLEIIWIK"
     gene            complement(1154..1408)
                     /locus_tag="VI935_12110"
     CDS             complement(1154..1408)
                     /locus_tag="VI935_12110"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HLE26372.1"
                     /translation="MKSSVGERGQIVIPKQIRDRLRLEKGMILEVETSGDDGAIVLRP
                     IRKRRKDWRDWIGVFAGDKLTQKYLRDKKKEKEKEVRWIK"
     gene            complement(1590..1844)
                     /locus_tag="VI935_12115"
     CDS             complement(1590..1844)
                     /locus_tag="VI935_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019951959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HLE26373.1"
                     /translation="MGIISWIILGLVAGVIAKWILPGRDPGGIVLTIVIGIVGAVIGG
                     YIGTLLGFGKATGFNLHSLVIAVAGSIILLLIYRMFARRA"
BASE COUNT          630 a          424 c          339 g          666 t
ORIGIN      
        1 tgatgaacta aaggaaagac tagaatcttt atcacaaaaa tttaaggatt tccgacaaac
       61 taagcttggt aaaaccattt attggttggc atctaatagt cttggattca ttccatggat
      121 tgggacaccg gcatcccttg gcctgagctt ctacgacgaa tttttggtaa aagacttttt
      181 gccaaaaggt ggagctatga cattcataaa caacaaattg ccaactgttt ataaagaaac
      241 tcaagaagtg aaaccttgag ggctaaatat aactactagc agaggtaaac aaaaatgata
      301 actgtgccta aactcacatt agatgatgca aaaattattg ttgatggagc tgagaaaaaa
      361 gcaaaagaga taggtgttga tatggacatc gcagttgtgg atgatggtgg gaatctacta
      421 gctttttata gaatggataa ggctaagatt acaagcattg atgttgcaat taacaaagca
      481 tttacagcgg ccggcgctag aaaggcaaca catgaatacg cagagatcgc tggggctggc
      541 ggtcccgcct ttggtatcca tgttagtaac cgaggaaggt ttatgatatt cggcggtgga
      601 cttccgattt tcgtagatgg tcacatagta ggaggagttg gatgcagttc aggcactgtt
      661 gaagaggaca gaatcgtagc ccaagcaggg attgataccc taatgaaaca cttgaaaaag
      721 tattagagga taaaaaatac atacttaatt gatttctttt tataatttta cttaatccat
      781 attatctcca atccatttac cgctttaaat tcctcatctc ctgttatcac ttttgcttca
      841 agctccatag ccaaagctat acaaaaagca tccgcatatg aaactgggta ggtagccttt
      901 actttagatg cggatcttat cctccttgcg gttggctcta aaaaaacaat cggaaagctc
      961 cttctttctt cccaaaactc ctccgcgtct ccagttttcc ctcttcttat aagtgtataa
     1021 taaacctcgc cccaatttat caggcttata taggctctta cttttccact gtaacttctt
     1081 tcaagatagt cttttacaat ttcatatcca ttttctttcc ctaacaaagt aagtatagca
     1141 aatgcatcca gaactacttt atccatctaa cttctttttc cttttctttc ttcttatccc
     1201 ttaggtattt ttgggttaat ttgtctcccg caaaaactcc tatccagtct ctccaatctt
     1261 ttcttctttt tcttataggc cttaacacta tagcaccgtc atctccactg gtctcgacct
     1321 ctaatatcat acccttctca agcctgagtc tatcccttat ctgcttaggt attacaatct
     1381 gtcccctttc tcctactgat gatttcatat acatacctcc tatgtatgaa tataaatttt
     1441 aaaattaaaa aggtcaatat gctaaagtta gaatttaata tatcagttta ttaagcttta
     1501 tcgtacggtt atttaatcag ttttattaga cttaagcgtt agcgtattat gaatttgccc
     1561 tcttaaacca tccgagtcta caaattcact tatgcccttc tggcaaacat acggtagata
     1621 agaagaagaa tgattgaacc agctacggct ataaccaagc tatgcaaatt aaaaccggta
     1681 gctttaccaa acccgagaag cgttccaata tagcctccaa tgactgcacc aacgattcct
     1741 attacaattg ttaagacgat ccctccaggg tctcttcccg gaagaatcca cttagcaata
     1801 acgcctgcaa ctagtcccaa tattatccaa gaaataattc ccatgatagt tcctcctttc
     1861 atttgaatag gattaagaca agcgctatta ttatctatta aagattaagc acttgtcaat
     1921 aaaccgttga tcatatgtgt tgataacatg ctaattattt tatgtgcctc ttccctttaa
     1981 tttatctatt atatttctaa agttgagttg caatagcggt aataagttta gtatttctat
     2041 cataattgtc ctgcttata
//
LOCUS       DATDRL010000365         4133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3324214, whole genome shotgun sequence.
ACCESSION   DATDRL010000365 DATDRL010000000
VERSION     DATDRL010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3324214"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VI935_12120"
     CDS             complement(<1..788)
                     /locus_tag="VI935_12120"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26374.1"
                     /translation="MNLKKSRSEKGIVLIIVLIVIAILTTLVVDLMYFTHIDIEISSN
                     TRDELKSRYIAKSGVYVIAGTLKNEPLEEITTFASNFGDQVGDSKGYWAIQIPFLPFG
                     DGSLSIKVIDERSKINLNALINQTTNDVDRQVHAELTELFRMLGVDNGKSSLFIASLT
                     NWLDRPISGSRNDQNPAGANGDFYAGLENPYRIKDGSLDSLEEIRQIHGMDDEFFNLI
                     KDYVTVYPADKKINFSTAPKIVIKAAVKGSAVSAKQGQESASPE"
     gene            complement(819..1562)
                     /locus_tag="VI935_12125"
     CDS             complement(819..1562)
                     /locus_tag="VI935_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HLE26375.1"
                     /translation="MKSHWRCLISNRGSLNTLPLDFVVSSSTSSLQAQSNDRDPRSLG
                     FTLIEFLLAVFIGAIVLTVLYASFFQIINAKDSAENELELYHEARVVHSKITEDLVMA
                     FPMGKIYSVKVSSPSSFFVGEVEGKNSSVAFTSLSHKRGINSPDSDQAVISYFLEPIP
                     ESSLFFLMRGENPRIGSTSVAIQYPLSESVVGFNLTYLSGDGEEFVDVWDSSQTNSLP
                     KAVEVTLTMRSRRGEDVVFNSLILIPSAN"
     gene            complement(1528..2040)
                     /locus_tag="VI935_12130"
     CDS             complement(1528..2040)
                     /locus_tag="VI935_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HLE26376.1"
                     /translation="MSILNYSRNKGVISTVDCRSCIENRGLCIELRITSHESRTPKSQ
                     IRNTQYTITGFTLLEVLVAVAILGAALAVLLGAVNKDLILASQSKNLVIASALAQRKL
                     SEIELKGFPEEVEEEGEFTEAPGFKWYSSVTHLGLPGLDTEIRVVRLLITWDEGKKDY
                     EVTLAMSNLK"
     gene            complement(2012..2521)
                     /locus_tag="VI935_12135"
     CDS             complement(2012..2521)
                     /locus_tag="VI935_12135"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HLE26377.1"
                     /translation="MRDQKGFTLIELVVVIFLVGVFFFVAIPKFKDITEVNIKSASRR
                     LTGTIRYLYSEAVFKKRVYKLVFEIDTSEYWVEVIEGNEFVAPADTFLQRKRLPVGVF
                     FKDITTQRSLGKTEKGSGEFILFMPTGYVEPAVIHLVTEDGQAYTLATKPYTGGTIIF
                     DEYIELFQK"
     gene            complement(2518..2946)
                     /gene="gspG"
                     /locus_tag="VI935_12140"
     CDS             complement(2518..2946)
                     /gene="gspG"
                     /locus_tag="VI935_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HLE26378.1"
                     /translation="MRSEGGFTLIEIIVVLVIIAGLAYVVSTNVIGRLEKARVETTKI
                     QIKQLETGLKQFKLDNAFYPETQQGLEALIAPPTTGRIPQRYAREGYLEGGQVPKDQW
                     GNDYVYIGPDQTQDSSYEIISPGEDGVYPSDDDISSRNIQ"
     gene            complement(3061..>4133)
                     /locus_tag="VI935_12145"
     CDS             complement(3061..>4133)
                     /locus_tag="VI935_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLE26379.1"
                     /translation="KGKTRRLFIFKGISTAELAVTTRQFSTLISAGFPLEASLVALSE
                     QTGDARMSQVLTQVRDRISEGSSLASALKEHPNVFSDLYINIVKAGEASGTLDIVLLR
                     LSDFLEKQMALRSRVRSALVYPIFMFFIGGGVLFFMMTYVIPRIAKIFEESRKALPLI
                     TVVLIEISKFLSQNLFALFIFLIGLFFIAYRFSKTERGKMFFDRLTLRLPIFGRLARM
                     ILISRFTRTLGTLLASGIPLLDAIEIGEAVVGNRVYVETLKGVRENVREGTSLAEPLR
                     ESGIFPPLVTRMITVGEQTGELEEMLTKIADTYDLQVEITVSTLTSLLEPVMILLIGA
                     VIGFIVFAILLPIFDLTSTIGR"
BASE COUNT         1206 a          933 c          751 g         1243 t
ORIGIN      
        1 tcttcgggcg atgcgctttc ttgtccttgt tttgcagaga ctgcagagcc cttgacagcc
       61 gcctttatca caatcttggg ggctgtgctg aaatttattt ttttgtcagc aggataaact
      121 gtgacgtagt cttttatcaa attaaagaat tcatcatcca ttccgtgtat ctgtctaatc
      181 tcttctaaac tgtcgagcga accgtctttt atccgataag gattttcaag acctgcatag
      241 aagtccccgt ttgcacccgc aggattttga tcatttctcg agcctgatat cggacggtca
      301 agccagttag ttaaacttgc aataaaaagt gaagacttac cattatccac accaagcatt
      361 ctaaaaagct ctgtaagctc agcatgcacc tgacggtcga catcattagt ggtctgattt
      421 ataagtgcat ttaggttgat ctttgaacgc tcgtcaataa ccttgataga aagggagcca
      481 tctccaaatg gtaaaaaggg gatctggata gcccaatacc ctttagaatc tcccacctgg
      541 tcgccaaaat tactagcgaa tgtggtaatc tcctcaagtg gctcgttctt tagtgtccca
      601 gcaataacat aaaccccgga ctttgctatg tagcgcgatt ttaattcatc ccttgtatta
      661 gaagatattt ctatatcaat atgtgtgaaa tacataagat caactacaag tgtcgtaaga
      721 attgcaataa caataagcac tattattagt acgatgccct tttctgatct actcttcttg
      781 agattcatat tcttaaaatt aatgcttctt attaattttt aatttgcaga aggaattagt
      841 attagagaat taaacactac atcttctccc ctcctactcc tcattgttaa tgtaacctca
      901 actgcctttg gaagggagtt tgtttgggat gaatcccaaa catctacaaa ttcctcccca
      961 tcacccgata ggtaagtaag gttaaacccc actacgcttt cggatagtgg atactgaatt
     1021 gcaacagagg tgcttccaat tcttggattt tcacctctca taagaaaaaa aagactgctt
     1081 tcaggaattg gttcaagaaa gtaactgatc acagcctgat ctgaatctgg agagtttata
     1141 cctcttttat gggataggga ggtaaaggca actgagctat tctttccttc tacctcacca
     1201 acaaaaaatg aagacggaga actaacctta actgaataaa tcttacccat tggaaatgcc
     1261 ataaccaagt cctcggttat cttggagtgc acaacccttg cctcatggta gagttcaagc
     1321 tcattttcag cggaatcctt tgcgttgatt atctgaaaga aggatgcgta aagaacggtt
     1381 aaaactatag cccctataaa aacagcaaga agaaattcaa ttagagtaaa ccctagcgat
     1441 ctggggtcgc gatcgttcga ctgagcctgt agtgagcttg tcgaactgct cacaacgaag
     1501 tctagcggga gggtatttag ggaacctcta tttgagatta gacatcgcca gtgtgacttc
     1561 atagtctttt ttaccctcat cccaagtaat taataatcga acgaccctta tttctgtgtc
     1621 taaacctgga agacctaaat gggttactga actgtaccat ttaaaaccag gagcctctgt
     1681 aaattcgccc tcctcctcta cttcttctgg aaaccctttt agttcaattt ctgaaagctt
     1741 tcgttgggcg agggcgctcg caattactaa attctttgac tgggaggcaa gaattaaatc
     1801 tttatttaca gcccctaaaa gtactgcaag tgcggcgccg agtattgcaa ccgctactag
     1861 aacctcaaga agagtaaacc ctgtgattgt gtattgagta ttgcggattt gagatttcgg
     1921 ggttcgtgat tcatgactcg tgattcttaa ctcgatacac aaaccacgat tctcgatgca
     1981 cgacctgcaa tctacagtcg agataacccc tttatttctg gaatagttca atatactcat
     2041 cgaatataat agtccccccc gtgtagggtt tcgtagctag tgtgtaggct tgtccatctt
     2101 cagttacaag atgaatcaca gccggctcaa catacccggt cggcataaat aatataaatt
     2161 ccccacttcc cttctctgtc tttccaaggc ttctctgtgt tgtaatatct ttaaaaaaaa
     2221 cgccgacagg aagcctcttt ctttgaagaa aggtatcagc aggcgctaca aattcatttc
     2281 cctctataac ttcaacccaa tactcacttg tgtctatttc aaataccagc ttatagaccc
     2341 tttttttaaa aacagcttca ctatataggt atcttatagt accagtcagt cttctcgagg
     2401 cactttttat atttacctct gttatatctt taaattttgg aattgctaca aaaaagaaaa
     2461 caccaaccaa gaaaatcact acaacaagct ctataagggt aaaacctttt tgatccctca
     2521 ttgtatattc cggcttgaaa tgtcatcatc acttggatat acgccatctt ctccaggaga
     2581 tatgatttca taagagctgt cttgagtttg atcaggtcca atatagacat aatcgtttcc
     2641 ccattggtcc ttaggaactt ggccaccttc gagatatcct tctcttgcgt atcgctgtgg
     2701 aattctccca gtagtgggcg gagctattag agcctcaagc ccttgctgtg tctctggata
     2761 aaaggcgtta tcgagcttaa actgttttag accagtctcg agctgtttga tttgaatctt
     2821 tgtggtttca accctggcct tttcgagtct cccaataaca tttgtgctga caacataagc
     2881 aagacccgca attataacca gaaccacgat gatttcaata agcgtaaaac caccttctga
     2941 cctcatttta aacctcctaa acaaaatgat gcataattca tgatacatga tgcacgattt
     3001 atcctatatc ttaggtcttg catcttgtat cctgcatctt gaatcgtgct ttttaccctt
     3061 ctatctacca attgttgaag ttaaatcgaa tataggaaga agaattgcaa aaactataaa
     3121 gccgatcacc gcacctatta gcagtatcat aacaggctca aggagagagg taagagtaga
     3181 aaccgtaatt tcaacctgta gatcgtaagt atctgcaatc ttagttagca tttcttcaag
     3241 ctccccagtt tgctctccta cggttatcat tcttgtaaca agtggcggga atattccgct
     3301 ttctctaagt ggctctgcaa ggcttgttcc ttctcttaca ttttcacgga caccctttaa
     3361 tgtttcaaca tacaccctgt ttcctacaac tgcctctcct atttcaatcg catcaagaag
     3421 gggaatccca cttgcaagaa gggtcccaag ggtgcgtgtg aatctcgata ttaaaatcat
     3481 ccttgctaac cttccaaata taggaagcct aagggttaaa cgatcaaaga acattttccc
     3541 gcgctcagtc ttactgaatc ggtaggcaat aaaaaaaaga ccgattaaaa aaatgaatag
     3601 agcaaaaagg ttttgactca agaatttact aatttctata agcactactg taataagtgg
     3661 aagggccttt cggctttctt caaatatctt cgctatcctg gggataacat aggtcatcat
     3721 aaaaaataga accccacctc ctataaaaaa cataaagatc ggataaacaa gggcgctcct
     3781 tacgcgtgag cgaagagcca tctgcttttc aagaaaatca gaaagcctaa gaagcacaat
     3841 atcgagtgtt ccacttgcct cacctgcttt tacaatattt atataaaggt cagagaacac
     3901 gttaggatgc tcctttagcg cagaggctaa ggagctcccc tcacttatcc tatctcttac
     3961 ctgagttagt acctggctca ttcttgcatc tccagtctgt tccgatagcg caacaaggga
     4021 tgcttcaagt ggaaatccag cagaaataag agtagaaaac tgccttgttg ttactgcaag
     4081 ctctgccgtg ctgatcccct taaatatgaa taaccttctc gtctttccct ttt
//
LOCUS       DATDRL010000366         4859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3328392, whole genome shotgun sequence.
ACCESSION   DATDRL010000366 DATDRL010000000
VERSION     DATDRL010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3328392"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1032)
                     /locus_tag="VI935_12150"
     CDS             complement(196..1032)
                     /locus_tag="VI935_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013202224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HLE26380.1"
                     /translation="MIAYLILVHRYPRQFKRLFRAIFHPANYYLVHVDKRSGVGLQTE
                     IQDFLSSFANASLLKSRNTLWGGYSLVDAELRGIEELLKIGSEWEFFINLSGQDFPLK
                     SQTHIQDFLGRNIGNDFIKVANQSKIRPDTLSRIEDYCIEFSNSVLRIPIKRQYLRGV
                     TPYIGNQWMILSRKLCEHICYNPEVKRFKRFYRHTFIPDEGFFQTVIMNTSYEGTVLN
                     DDKRTIVWVPIGTIKLRPKDFTSKDAEFLLSSQGLFARKFDETVDAGILSILELNLSS
                     QF"
     gene            2065..4266
                     /gene="purL"
                     /locus_tag="VI935_12155"
     CDS             2065..4266
                     /gene="purL"
                     /locus_tag="VI935_12155"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HLE26381.1"
                     /translation="MDNLKLALNHGLKEEEYKKIVENLGREPNPTEIGIFAAMWSEHC
                     SYKSSKVHLKKLPTKGERVIQGPGENAGIVDIGDGICVAFKMESHNHPSFIEPYQGAA
                     TGVGGILRDIFTMGARPIALLDSLRFGDPKLPKTKYLVEGVVSGIAGYGNCIGIPTVG
                     GEVYFEECYNGNPLVNVFALGVTRRNEIFRGYASGIGNPVIYVGSKTGRDGIHGAVMA
                     SETFTREAEEKRPAVQVGDPFTEKLLLEACLELFKTGVVVGIQDMGAAGLTSSSVEMA
                     GRGGTGITLNIDKVPRRETAMTPYEVMLSESQERMLLVVQKGKQEVAEKIFRKWALDF
                     AVIGEVIDSQRLKVIENGKIAVDLPIKPITSEAPVYRRPCRKPVYQRKVNNLKLSSLP
                     IPNDMNAVLLGLISSPNLCNKRWIYEQYDYMVRTNTIVTPSSDAAVIRIKGTDKALAL
                     TSNCNSRYCYLDPYAGAAIAVAESARNLACSGAKPLAITDCLNFGDPEKPEIMWQFKE
                     SVLGMAYAAILLGTPVVSGNVSFYNETENKAIYPTPTVAMVGLIENQRKHITQWFKKQ
                     GDIIVLLGETKEELGGSEYLKLIHELVSGPPPYIDLEAESRLINTLLEACEKGTINSA
                     HDLSEGGLAVAIAESCFTPNGPAGVKINPFYGRIREDAFLFGESQSRVIVSLDRKNIR
                     ILENITSKFGVPMEVIGEVGGSSLNISDLINIQISKAYETWAFGFEDVLRQ"
     gene            4383..>4859
                     /locus_tag="VI935_12160"
     CDS             4383..>4859
                     /locus_tag="VI935_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HLE26382.1"
                     /translation="MPKLKEECGVFGIYGHPEAANLTYLGLHALQHRGQESAGIVTSN
                     GTNLFTHREMGLVNDIFTEEALNKLPGRNSIGHVRYSTTGSSHLKNSQPIVVTYARTE
                     LAIAHNGNLTNALTLKEELEQSGAIFQSTTDTEVIVHLIARSREQSLIKRIIEALMR"
BASE COUNT         1457 a         1010 c         1080 g         1312 t
ORIGIN      
        1 gactttataa ctttggttgt atgtattctc ccaattgcat ccttagattt aattctacag
       61 gttcgaggta gaataattca agccactgac ttagattaca attctttggt tcggacttcg
      121 gcataacgtg acaagctcaa ccgcgcggta gcgcggtcgg ctccagccgc gtgttagccc
      181 gctctccgtg ataagtcaaa attgcgatga tagattcaac tccaatatac tcaagatccc
      241 tgcatcaaca gtttcgtcga atttacgggc aaacaagcct tgacttgata gcaagaattc
      301 ggcgtctttg gatgtgaagt ctttgggacg taactttatt gttcctatcg gcacccacac
      361 aattgtcctt ttatcgtcgt tcaggacagt tccctcgtag ctagtattca ttattaccgt
      421 ctgaaagaat ccttcatcag gtatgaaggt gtgccgatag aatctcttga accttttgac
      481 ctcagggttg tagcatatat gttcacataa cttcctgctc aagatcatcc actgatttcc
      541 gatataagga gttacgcctc gaagatactg tcgcttgata ggaatgcgta gtacactgtt
      601 gctaaactcg atacagtagt cctcgattcg agatagtgta tcagggcgta tcttgctctg
      661 gttagccacc ttgatgaaat cgtttcctat attgcggcca agaaagtctt ggatgtgcgt
      721 ctgcgacttt agggggaaat cctgtccgct aaggtttatg aagaattccc attccgagcc
      781 gatcttcaat aactcctcga tgcctcgcaa ttcagcatct accaagctgt atccccccca
      841 cagagtgttt cggcttttca gcaaggatgc gttggcaaag cttgacaaga aatcttgtat
      901 ctccgtctgt aatccaacgc ctgacctctt gtccacatgt accaagtagt agttggcagg
      961 atggaaaatt gccctaaaga gtcttttgaa ctggcgggga taccgatgca ccaagatgag
     1021 gtatgcgatc atgtccttaa cgatctccta taatctgtat ctctcgaatt tttatcatgg
     1081 caagcctttc atggttaaga tatggctatg tcagaattat attccggata cgacggcttg
     1141 gccgaaaaat gggcgacgcc tttgcataca acgagcgggc taacggagcg gcggttgaac
     1201 ggtgtgagtg gggtgcttgc atcccagcga agcatccgct ccagccgctt gttcgacggc
     1261 gcaaagcgtc cgaactaact actgattagg cggatttttt tccgcataat caccctatac
     1321 ggagtgttat acggaaatcc ttgcgccttt tccctttctt tgggagtata cttaaagtgt
     1381 ctccctagtt caatagatac gagggaaact tatggaacaa cgccctaaga aacttcttaa
     1441 cctcatatgc gaagttatcc aactaaaccg tacactctga tttgtttaac agatatattg
     1501 tcaaatctag ggaaatttcg gaaaggataa tttaaaacgt accacactta aaacccaact
     1561 tgagccgccg cttgttacgg agctcgctct cagattcaat gagtccagac gacgaagcga
     1621 atgtcagtaa gtatctgcga aacccgcagt tgatcgtgct atttcttctt cgaaccttgg
     1681 gccttgctct ttcgttgtac atcacgtcta tgagcgcgct cgtacgtctt tatgtttacg
     1741 aacctgccct tcttatctcg gaccgcgtaa tacttcttgc ccgaactgct tcgttgagtc
     1801 gtcctctttg tagccattct ctcctcctct tgccatgcct ggctattaat tagcctggaa
     1861 tggtgcagta taatacctca aagcgcatgt tataccgcaa attatagtta acccgattgt
     1921 cacacgtata atagaaaaag tcaatagaaa aattaaaaac agtcccaaaa aaagcttgtt
     1981 gacccaatac gtgatactat ctgactgtgc catacactac aacttatcta acaggtatat
     2041 ttacgaatca aggaaagtgc ctgaatggat aatctaaaac tcgctctaaa ccacggactt
     2101 aaagaggagg agtataaaaa aatagtagag aacttgggca gagagccaaa tcctacagaa
     2161 atcggtatct ttgcagctat gtggtctgag cactgttcgt ataaaagctc taaggtgcat
     2221 ctaaaaaaac tgccaaccaa aggggaaagg gtaattcaag gtcctgggga aaatgcaggg
     2281 attgtagata ttggtgacgg catttgtgtt gccttcaaga tggaaagcca caatcatccc
     2341 tcttttattg aaccctatca gggggcagca acaggtgttg gtggaatcct gagagacata
     2401 tttactatgg gagcaaggcc cattgcgctt cttgattctc ttagattcgg agacccaaag
     2461 cttccaaaga caaaatatct ggttgaaggt gtagtttcag gaattgcagg atatggaaat
     2521 tgtatcggca tcccaacagt tggcggagag gtttatttcg aggaatgtta taacgggaac
     2581 ccacttgtaa acgttttcgc acttggtgta accaggcgaa atgagatttt caggggttat
     2641 gcaagtggta taggaaaccc agtcatatat gtaggatcaa aaacaggaag agacggaata
     2701 cacggcgctg ttatggcgtc agagacattc actagagaag cggaagaaaa aagaccagca
     2761 gtacaggtag gggacccatt tacagaaaag cttttactag aagcctgcct cgaacttttt
     2821 aagacagggg tagtagttgg tatacaggat atgggggcgg cagggcttac atcctcttct
     2881 gtcgaaatgg cgggtcgtgg aggaacaggc attacattga atatcgataa ggtaccaagg
     2941 cgagagactg ctatgacccc ttatgaagtt atgctttcag aatcccagga gagaatgctt
     3001 cttgtagttc aaaaaggaaa acaggaggtt gctgaaaaaa tattcagaaa gtgggcgctg
     3061 gattttgctg ttatcggaga ggtcatagat agccaaagat taaaagtaat tgaaaatggt
     3121 aaaatagccg ttgaccttcc aattaagcca attacaagcg aagctcctgt ttatagaaga
     3181 ccatgtagaa aaccagttta tcaaagaaaa gttaataacc tcaagctatc ttctcttcca
     3241 attcctaatg acatgaatgc tgttctcctt gggctcattt cttctcctaa cctttgtaat
     3301 aaaagatgga tttatgaaca gtatgattac atggttagaa caaacacaat cgtgacacct
     3361 agttcagatg ccgcagtaat aagaatcaag ggaacagaca aggcattagc actaacatca
     3421 aactgcaact caagatactg ctatcttgac ccatatgctg gtgcagctat tgctgtggca
     3481 gaaagcgcaa gaaaccttgc ctgctctggg gcgaaacccc ttgctatcac agactgccta
     3541 aattttgggg atcctgaaaa gcctgagatc atgtggcaat ttaaggaatc ggtattggga
     3601 atggcatatg cggcaatact attaggaacc ccagtagtaa gtggtaatgt tagcttctat
     3661 aacgagactg agaataaggc catataccca accccgactg ttgcaatggt agggcttatc
     3721 gaaaaccaaa gaaaacacat tacccaatgg tttaaaaagc agggtgacat tatagtgctt
     3781 cttggagaaa ctaaggagga gctcggtggt agtgaatatc tgaaactaat tcacgaatta
     3841 gtttcggggc cacctcctta tattgattta gaagcagaat cgagacttat aaacaccctt
     3901 cttgaagcat gtgaaaaggg aacaatcaac tcagcacacg atttatctga aggtgggtta
     3961 gcagttgcaa tcgccgaatc ttgttttaca cctaatggtc ctgctggagt gaagataaac
     4021 ccattctatg gtagaattag agaagatgct tttctatttg gagagtctca gtcaagagtt
     4081 atagtttcct tggatagaaa aaacattcgc atattagaga acatcacttc aaaatttggg
     4141 gttccaatgg aggtaattgg tgaagtagga ggaagtagtc ttaatatctc cgatctgatt
     4201 aacattcaaa tttcaaaagc atacgaaacg tgggcttttg ggtttgagga cgttttacgg
     4261 caataaattt agctacgaaa taacacggat gaacacgaaa agaaaaaact tgaatgggaa
     4321 ggatttaaaa aattatttta tttgggtaaa ttcgtgccca ttcgtggcta atttaaaacg
     4381 ctatgcctaa actcaaagaa gagtgcggag tttttggtat atatgggcac cccgaggctg
     4441 caaacctcac ctatcttggg cttcatgcac ttcaacaccg tggacaggaa agcgctggaa
     4501 tagtcacatc aaacggaacg aatctcttta cacatcgcga aatgggcctg gtaaatgata
     4561 tatttacaga agaggctctc aataaactgc ctggaagaaa ttcaataggc catgtaagat
     4621 actcaactac cggctctagc caccttaaaa actcacagcc tatagtcgtt acatatgcgc
     4681 gcactgagct tgcaatcgcc cataatggta accttacgaa cgcattaaca cttaaagagg
     4741 aactcgagca aagcggggcg atctttcaat ctacaactga tacagaagta atcgtacatc
     4801 taattgctag atcaagggag cagagtctta taaagaggat aatcgaagcc cttatgaga
//
LOCUS       DATDRL010000367        17179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2277688, whole genome shotgun sequence.
ACCESSION   DATDRL010000367 DATDRL010000000
VERSION     DATDRL010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 17179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17179
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2277688"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VI935_12165"
     CDS             <1..234
                     /locus_tag="VI935_12165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE26383.1"
                     /translation="PVITTNVTGIPEIVQDGVSGILVTPNDENALAEAMIKLIEDHKL
                     REKLGENARKKVEEKFDISRNIVKYVSLFKGVS"
     gene            282..1187
                     /locus_tag="VI935_12170"
     CDS             282..1187
                     /locus_tag="VI935_12170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019493219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE26384.1"
                     /translation="MKPFFSIIIPTYNRPIQLRNCLHSLTLLDYPRNCFEVIVVNDGG
                     ETSPEAVVDPFHNQINLSFITHTQAGPAAARNSGAGKAMGEFLAFTDDDCMADPDWLK
                     TLAARFKEATDCIIGGRTINHLTNNIYSTTSQLILDVVYGHYNAKPNQSRFFASNNMA
                     LPAHLYFTIGGFDPNFTTSEDREFCNRCLHLRFKMIYEPKAIIYHAHSLNLHTFSKQH
                     FNYGRGAFRYHLTRLRRGYGGFFEDLKFLLNFRNWLFYPFSQVQVSQVLTLAWLLIIW
                     QLVNAAGFFWEAGNHSIRNILRIPG"
     gene            1494..2468
                     /locus_tag="VI935_12175"
     CDS             1494..2468
                     /locus_tag="VI935_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26385.1"
                     /translation="MSDGSSPDISVILVTLDHYERIRKTIKYLRSQTVSDSMEVVIVA
                     PSSDKLGLNESELKDFLKFQVVEVGEIESTGGCIAAGVHKSCAPVISYVEEHVYPEAG
                     WAEALINAHRKPYAAVGAVIYNANPGSMISWASLFTDFGPWVEQSLSIKNRGLAPHQT
                     SYKRSVLLEYGTELKTMLEVETILHRDLLSKGYQLYLEPAAKVNHLNPSLLSSYLGSE
                     FQGGRLFGTARAHYEHWSIGKRLLYIIGMPLIPLIRLRRVLKEIRRTAVKPNLLPSIL
                     PALIMGLISHSLGEITGYAFGVGDAPQRRLTIELERSRHIKEQDKYRE"
     gene            2473..3465
                     /locus_tag="VI935_12180"
     CDS             2473..3465
                     /locus_tag="VI935_12180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE26386.1"
                     /translation="MNDLNYPDISVIIVTPDSFETISKTIDCLRKQTAKDRIEVVIVA
                     PSADRLNLNNSVLNDFPKMKVVQIGTIESRAVGNAAGVRQATAPVAVLAEDHSFPDPR
                     WAEALIRAHRQPWAAVGPVVRNANPDSALSWADLVISYGPWLDPTPAGVASHLPGHNC
                     SYKRGILLEYRDKLESMLEAESVLHWDLRSKEFQIYLEPEAKISHINFSLLSSWIRVR
                     FIAGRYFAASRSINWPLFKRIMYTVATPLIPFIRFSRTILNMYRPGRLQNLPLSMKLR
                     IVVAMILGLILDGAGQSTGYAMGYGDSKEKMLKYEFHRYRHLSKKDKVRYDFKD"
     gene            3449..4273
                     /locus_tag="VI935_12185"
     CDS             3449..4273
                     /locus_tag="VI935_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26387.1"
                     /translation="MISKTRLLKLVFNPSLILIGLWKKIPFGPFKLRLALDVFNRPHY
                     AYCTYHAALEAKLLGINRISVIEFGVAGGLGLLDLEEVSAHVSRETNIDIDVYGFDSG
                     TGLLNPLDYRDLPYVFQKGFYKMDIDLLKKRLKKAILVIGDVKDTVPEFFEKYKPAPL
                     GFVAIDLDFYSSTVHALGLFNTDDQNQLPRVFCYFDDLIGDDYALHSEFAGELLAIRE
                     FNDQSEYRKLGKINGLSYKRIINQRWWEQVYALHNFKHPLYSKYIYEKKDRQLILK"
     gene            4315..5388
                     /locus_tag="VI935_12190"
     CDS             4315..5388
                     /locus_tag="VI935_12190"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLE26388.1"
                     /translation="MTSLKLGLIGCGRIAQLVHLNVIRQLPNSELIALAEPDPKRREE
                     ASKLVPKAIAFESYNELLSMPEVEAVIICLPNNLHAEATVTALEHGKHVYLEKPLATS
                     LKEAQGVLEAWRRADLVGMVGFNYRFNAFYKAARQHIESGRLGELVCVRSVFSTATRT
                     LPTWKKARERGGGALLDLASHHIDLVRFISGEEIQEVFARVWLQQSEDDCAMLQLRLA
                     NGLLVQSFFSLSAVEEDRFEIYGMEGKLTIDRYLSFDVEITDPTAEFARLKWLGRMFQ
                     SLFHSPYLKERIFSPGSEPSYKAALNHFVTAVRKKQPTSPDFWDGYQSLAVIEAAEES
                     ARIQRVVSLADFVKEDFNYQYSE"
     gene            5463..7115
                     /locus_tag="VI935_12195"
     CDS             5463..7115
                     /locus_tag="VI935_12195"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HLE26389.1"
                     /translation="MKANNRGNKGSTDKDRIIVIGLDACDADLVEQLSKEGRMPFLTS
                     LMKSGLWVRLTSTKVFFNDSPWPSFNAGVSPAKHGYYNYLQIKRGTTEIERINAHHCR
                     YLPFWSLLCDSGKQVAVFDVPKTFPIEGIDGIQISAWSEHSLTLKQPTSNPPEFIRDI
                     ITHYGKYSHPREIITPRSTRQERRIYKTLLSNLEKKLQSTEFLMRQGDWDLFFTVFSE
                     GHTAGHQFYHRFNEGHWAYEPDAPDEIRNALLHIYTQLDAALARLFSRISDQTTFFIV
                     SVHGMAINYSANHMMPKVMEKLGFQVPATKKKQLNTIGSLLKRTTFARNLIPLSLRDF
                     INTHIVSETVHDKAVSSAFSSGIDWKKTKAFFLPSDHFQGYISLNLKGREPFGIVEHG
                     AEFDELSDEIRYELKHLTNPETGKPAVHDVVRTSDIYQGPNLYELPDLVVLWAEDAPI
                     NQLQHPRFGVISAKGYKLRKAQHVTDGFMIAGGKYINKNAYLSGASTFDIAPTILYLM
                     EQVIPDEMDGKVLLELIEEDFKRNNEPIYGKRPAIIPKAMRT"
     gene            7257..8150
                     /locus_tag="VI935_12200"
     CDS             7257..8150
                     /locus_tag="VI935_12200"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002758863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HLE26390.1"
                     /translation="MLIPNFLIIGSSRCGTTSLYRYLKQHPQIYMSPVKEPSFFSYEG
                     MKPNHRGPSDEKFNRTAVTKIEDYGALFKDVTTETAIGEASTNYIYNPKASERINHYI
                     PNARLLAILRHPVERAYSSFLYLIRDGREPLRDIAQAIEEEPQRIRQNWAPIWNYKHM
                     GFYYDQLKRYFDQFDRSQIRVYLYDDFSTNPSRLLQDIFQFIGVVDTFVPDTSLRHNV
                     SGIPRSRVLHKLLTQQSMIKYSVERLIPEGWRRRLSLQKRNLIRPTLPADLRRQLIEE
                     YREDILKLQELIQRDLSKWLE"
     gene            8268..9467
                     /locus_tag="VI935_12205"
     CDS             8268..9467
                     /locus_tag="VI935_12205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLE26391.1"
                     /translation="MKILLVNDYGTPTGGTELLTLALRDGLRERGHDARLFTSSARPG
                     IWDSHADYECIGTTSRFRTLLQTANPWAYWQLRRVLAEFQPDVVHVGIFLTQLSPLIL
                     PLLKDVPSLYHAHWYRPICPIGTKTLPDGNPCEVPAGAVCYHNRCLPLRDWLPLMLQM
                     KLWRRWRNSFNLIVANSEAVKSRLVEEGIEPVEVVWPSIPTRPLRPPLSTPQTVAFAG
                     RLVREKGVDVLIKAFAIVAKQIPVSKLLIAGDGPEWKSLNMQIKNLGLSSHVTMLAHL
                     SRPEVERLFDGAWVQVVPSRWAEPFGLVAAEAMMRGTAVVASASGGLSEIVQDGETGF
                     LVQPGNIGALAEALLKLLQDRALSEKVGKVGREIALAKFSEAAYVDRFIQLYQILCQS
                     RRKANDN"
     gene            9457..11004
                     /locus_tag="VI935_12210"
     CDS             9457..11004
                     /locus_tag="VI935_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HLE26392.1"
                     /translation="MTIDTKYSPLVILGIDAGDPVFIQHWAKEGCMPTVASIMNRGCW
                     GQTAGPELLSEHGVWKSLFSGISRSQHGYYYFRQLKPGTYDLKTVTGDDIEASPFWSY
                     LHGQDKKIAIIDAPDGRPLPGLPGIQLANWATHNNLDPHRFTTTSEPTELLEEVRRNF
                     GPKLVTMENHESTFDEDSRIYRQLMNQVEKKGALCRHLLASDRFDLIVIVFAESHVAN
                     HQFWKYYIEAKDGKTTEHNLTHAIRNVYKGIDREIGLLLKQLPNNSNVFTVSSVGMED
                     DYPTAGLIEAFCRKLGYQASSESNGGSFKPTNLVRRMVPESCRIALSRYLPREKRERL
                     LAEQFRSGTNWRKTTAFSIPASYTSFIRVNLKGREPEGIVEPGAEYMALLQRIEADLM
                     QLVDHQTNELAVTIVAKTVELFGCEPHVSLPDLFVEWKPGRFMQRVVHPRAELVQKKP
                     DFYRRSDHSSKGFVAAAGPYIHMRGALGNVEVLNLAPTFLYLMDEPIPRVMTGQVIKQ
                     IIMTDVP"
     gene            11037..11927
                     /locus_tag="VI935_12215"
     CDS             11037..11927
                     /locus_tag="VI935_12215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLE26393.1"
                     /translation="MWFRIARSAYRWFVSVVGRYYLKRMIEDGLPERLKLPLLFLLTK
                     SLSQEDQLIVSRIESVRSKMANRGDVYVDIYPSPSPGSVGMVTSPEVKPAPGKVKKVP
                     LSRIANTTSILPYWGTFLYLCANVNRATTILELGSCAGISGCYMASGKYCKRFITIEG
                     SRALATLAESNIRQVADNFAVITALFDDGFDETLPKLKDGLDMVYFDGQHEKIATIHY
                     LERLTPHLNAGSILILDDIRWTSDMWEAWQALCKWKGMAWTIDVGRFGICVWDGVATQ
                     PKNYDFSKFTDLWRKRKSRN"
     gene            12060..12914
                     /locus_tag="VI935_12220"
     CDS             12060..12914
                     /locus_tag="VI935_12220"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015956155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HLE26394.1"
                     /translation="MLPNLIIIGAQKCGTTRLHYYLGLHPQIFMSKEKELEFFIEERN
                     WPNGLAWYASNFKGRAKIYGESSPNYSYYPRFTGVPARMHAIEPKAKLIYIVRDPIER
                     MISSYIQNCSGGRENRSIEEALRSPNNQYRYRSLYYMQLMQYLAHYPASQIVVITNED
                     LECNLPSTLRKVFEFLDVDPDFCSRWYALRRHSTARKRRKTGIGMRLTQTPPMKLLRR
                     IPLRLRWPIEDLLYFPFSRKIGRPVLDDSLRQELIDYLRDDINRLREFTGNNFENWCL
                     EFYRHMNI"
     gene            13022..13933
                     /locus_tag="VI935_12225"
     CDS             13022..13933
                     /locus_tag="VI935_12225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLE26395.1"
                     /translation="MEEKQPFFSIIIPTYGRPSQLAACLQSITRLHYPGHSFEVIVVD
                     DDSKMPPEAVVASFQDQLNVTLLKQIHSGPAAARNTGAARAKGEFLTFIDDDCAPASN
                     WLQTLAARFAKSPNSAIGGRTINALPNNLYSTASQMLIDYLYTYYNADHNQGRFFTSN
                     NLAMPIVKFNMLGGFDTGIPRAGGEDRDLCDRWLHHGYQMIYAQEVLVYHAHALTLDT
                     FWRQHFNYGRGAFYFHQAHARRGQERIKVEPLPFYLNLLRYPFSPARGLQTLLLAVLL
                     GVSQMANAIGFLWERMLQTKASRWRIL"
     gene            14082..15377
                     /locus_tag="VI935_12230"
     CDS             14082..15377
                     /locus_tag="VI935_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26396.1"
                     /translation="MKTAVNYLLGSWSRTSTYMLRTWWDTHGSMLMRIAIALMVTVAI
                     AWLGYQFWRLLWQQDPIWPTSPSGAVDLNLRYKELHRWFAGEPVPPVYPPASYIILWP
                     LLGWLPFTPVKWFWAVTTVAALVWLVFLVVKESGANTAIERVFVALMPISMYATGATI
                     GNGQLIIHILPAILAGLLILQNRNCTFGKELLAVALIIFSLVKPNVSIPFLWVAILAP
                     GRLRPALLVALGYIALTLFALSLRETPEINLLSPENIQESPQDIPNITIPESFADITK
                     LYTWSATWAERDRYSDLPVLLTTLGLKAWIIPVTLFIMALQGLWTFRYRNGDIWLLVS
                     VAAIIARVWAYHRWFDDLLILLPMVALFRIANRERSTNSRKVVAGVLLAITLLSTLAP
                     GGLYLFPPPWNSLYAVGQSVIWVIVLIFLLDQARCEKKA"
     gene            15834..16889
                     /locus_tag="VI935_12235"
     CDS             15834..16889
                     /locus_tag="VI935_12235"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLE26397.1"
                     /translation="MEYQPFISVIIPVYNGCKYLNRCLDALMTSSYRQFEIIVVDDAS
                     TDDSVDICRKKGIAVVQMPHQSGPAAARNYGAQKARGDVLLFIDSDVLVQQETITLVA
                     ADFINNPDIVAIFGSYDDSPDASDFLSQFRNLFHHFIHQNSHQEAKTFWTGCGAIYKE
                     IFYQLKGFDEDWFSIEDIELGHRIWDNGYRIMLDKEIQVKHLKQWGLKNWLKTDILYR
                     AVPWSQLILSSGYMPWDLNLQTPHIISAVLVGLLTLTTFLIFLNPINLFGVHLNVILL
                     ILSFLLIFTIIILNRGLYGFFLQKRGIKFTILAIPIHFIYYFYSGVAFTLSWTLNKLD
                     NLIPGFRKFNRSHKRKA"
     gene            16995..>17179
                     /locus_tag="VI935_12240"
     CDS             16995..>17179
                     /locus_tag="VI935_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26398.1"
                     /translation="MGLLVMKFANSSTSRLRIWWHKHASMLLSFAVALMSVFALMKLG
                     EEFWRLTLDSSSIGAIDL"
BASE COUNT         4925 a         3657 c         3768 g         4829 t
ORIGIN      
        1 ccagtcatca cgacgaatgt aacgggtata cctgagattg tacaagatgg agtcagcgga
       61 atattagtca ctccaaatga cgagaatgct ttagcggaag caatgataaa gcttattgaa
      121 gatcataaat tgagagaaaa actaggggaa aatgcaagaa agaaagttga agaaaaattt
      181 gacataagta gaaatatcgt taaatacgtt tctttattta aaggagtaag ttaaagaata
      241 atctgacgtt ttgtattaga cacggttttc ggaacttttg catgaagcct ttcttctcaa
      301 ttattattcc gacttacaac cgcccaattc aattaaggaa ctgccttcac tcccttactc
      361 ttttggacta tccacgcaac tgctttgagg tgatcgtagt aaacgatggc ggagagacgt
      421 caccagaagc tgtagttgat ccattccata atcagatcaa cctaagtttc atcacacata
      481 cacaagccgg tcctgcagcc gcacgcaata gcggcgcagg aaaagcaatg ggcgaattcc
      541 tggcttttac agatgatgat tgtatggctg accctgattg gcttaagacc ttagccgcac
      601 gttttaagga ggcgacggat tgcattatag gtggaagaac gataaatcac ttgactaata
      661 atatctattc gactactagc cagcttattt tagacgttgt ctatggacac tataatgcca
      721 agccaaatca gtctcgtttt ttcgcctcaa ataatatggc tttacccgca catctttatt
      781 ttactattgg cggatttgat ccaaacttta ccacctcaga ggaccgtgaa ttttgtaatc
      841 gctgtttaca tctaagattt aaaatgattt atgaacccaa agcgataatc taccacgctc
      901 attcactgaa cctacatact ttctctaaac aacacttcaa ttatggtcgt ggggcctttc
      961 gttatcacct aactcgcctt cgaagaggtt atggtggttt ttttgaagat cttaaatttc
     1021 ttttaaactt ccgtaattgg cttttctatc ctttctcaca agtgcaagtc tctcaggtat
     1081 taacacttgc gtggttgctt attatttggc aactagttaa cgccgctgga tttttttggg
     1141 aagctggaaa tcatagtata agaaacatct tgcgaatccc aggatagtgt aatttgcgtt
     1201 gtattaacaa caaacaatac tatgaagtta gtcattctat aaaatgaaac catttaataa
     1261 gttaattcat ttccaaaatg tatactcgta atgggataca aaaccaatat gataccatct
     1321 caagcatgtt tcataagcaa ttcaggcttt gatagttctt cgttcaattg atattctcat
     1381 cgttagattt catattaacc ggtctaactg cgtggaatga ctcttgaaga tctttcagcc
     1441 taatcatcga aaatcacgaa acaatgaaca agcttatcaa gggtgcaatt attatgagtg
     1501 atggtagttc tcctgatatt tcggtgatac ttgtaacact agatcactat gagaggattc
     1561 gcaaaacaat caaatatcta cggtcacaaa cagtcagtga tagcatggag gttgtaatcg
     1621 tggcaccttc atcagataag ctcggtctaa atgaatcaga gcttaaagac tttctaaagt
     1681 ttcaagtggt agaagtcggt gaaatcgagt caacaggtgg gtgcatagct gccggtgttc
     1741 acaagtcatg tgcgccagta attagttacg ttgaagaaca cgtttatccg gaagcaggtt
     1801 gggctgaggc actaatcaat gcacaccgaa aaccctatgc cgcggtaggg gctgtaattt
     1861 ataacgctaa tcctggaagc atgattagtt gggctagcct ttttacagat tttggaccct
     1921 gggttgaaca atccctgtca attaagaatc ggggattagc cccccatcaa acatcttata
     1981 aacgttcagt tcttttggag tacggaactg aactaaaaac catgctagaa gtggagacca
     2041 tactacacag ggatctactc tcgaagggat atcaactcta tcttgagcca gcagcaaaag
     2101 taaaccattt aaacccctca ttactatctt cttatttagg atcagagttt caaggaggac
     2161 gcttattcgg gactgcacgt gcgcattatg agcattggtc tatcgggaaa cggctacttt
     2221 acattattgg tatgccctta attccgctaa ttcgtcttag gcgtgttcta aaagagattc
     2281 ggaggactgc agtaaaacct aatctgctcc cttctatttt gccagcatta attatgggtc
     2341 taatttcaca ttcgttgggt gagataacag gctacgcttt tggagtgggg gatgctccac
     2401 aacgtcggct tacaattgaa cttgaacgtt cccgtcacat caaagagcaa gacaaatata
     2461 gagaataaga aagtgaacga tcttaattat cctgatatat cagtgataat tgtcactccg
     2521 gatagtttcg aaacaatcag taaaacaata gattgtctaa ggaagcaaac tgcaaaagat
     2581 cgaatcgaag ttgtgattgt cgcaccatcg gctgaccgcc ttaatctgaa taattcagtg
     2641 ttaaatgact ttcctaagat gaaagtggtt cagatcggca ccatagagtc cagggctgtc
     2701 ggaaatgcgg ctggtgttcg tcaggctact gcgcctgtcg cagttcttgc cgaagatcat
     2761 tcttttccag atccaagatg ggctgaggct ctaatcagag cgcacaggca accttgggca
     2821 gcagtcggac cagtagtaag aaacgctaat ccagatagcg ctcttagctg ggctgacctt
     2881 gtgattagtt atggcccctg gttagatcct acaccggcag gggttgcaag ccatttgccc
     2941 ggacataact gtagctataa acggggaata ttgcttgaat atagagataa actggaatca
     3001 atgcttgaag cagaaagtgt tctacactgg gatttgcgct caaaagaatt tcaaatttat
     3061 cttgaaccag aggcaaaaat ctcacatata aatttttctt tgctttcgtc ctggatacgc
     3121 gtgcgattta tcgcgggaag gtactttgca gcgtcacgtt caataaactg gccactgttc
     3181 aaacggatta tgtatacagt tgcaacccca ctcataccat ttatacgttt tagccgcact
     3241 attttaaata tgtatagacc gggtagactg caaaaccttc cactctctat gaaactccga
     3301 attgttgtag caatgatttt gggattgatt ttagatggag caggacaatc tactggttat
     3361 gctatgggat acggcgattc caaagagaag atgctaaaat atgaattcca tcgctatcgt
     3421 catctttcta aaaaggataa ggtacgctat gatttcaaag actaggctgc ttaagcttgt
     3481 tttcaatcct agtttaatat tgatagggtt gtggaaaaaa ataccctttg gcccatttaa
     3541 gttaagattg gcactagatg tatttaatcg cccccattat gcctattgca cctatcatgc
     3601 tgctctagag gccaaactat taggtattaa tcgaatcagc gtcattgaat tcggcgttgc
     3661 aggtggctta ggtctcttgg atctagaaga ggtttcagca cacgtttcaa gagagactaa
     3721 tatagatatt gatgtttacg gctttgactc cggcacaggt cttttaaatc ctctcgacta
     3781 tcgagacctt ccctacgtat ttcagaaagg attttacaaa atggatattg acttactcaa
     3841 aaaaagattg aagaaagcga ttctggttat tggcgatgtt aaggacacag ttccagagtt
     3901 ctttgagaaa tataaaccag ctccgcttgg ctttgtcgct atcgacctgg acttttatag
     3961 ttccaccgtc cacgcattag gcttgtttaa tacagatgat caaaatcaat taccgagggt
     4021 tttttgttat tttgatgatt taattggtga tgattatgca ctccattcag aattcgcagg
     4081 agagctgcta gcaatacgtg agtttaatga tcaaagcgaa tatagaaagc taggtaaaat
     4141 aaatggatta tcttataaaa gaataataaa tcaaagatgg tgggagcagg tatatgcctt
     4201 acataatttt aaacatccgc tttactcaaa atacatatat gagaaaaaag ataggcagtt
     4261 aatacttaaa taatttctgt gccttctaag aaactgaagg agaactagca gccgatgacg
     4321 tcactcaaat taggtctaat tgggtgtggt cgtattgccc agttggttca ccttaatgtc
     4381 attagacaat taccaaacag tgaactaatt gcgctcgcag aaccagatcc taaaagacga
     4441 gaagaagcta gcaagcttgt acctaaagcc attgcatttg agagttacaa cgaattgctt
     4501 tcgatgccag aggttgaagc tgtcatcatc tgcttgccaa ataaccttca cgctgaggcc
     4561 acagtaacag cattagagca tggtaagcac gtttaccttg agaaaccgct tgcgactagc
     4621 ctcaaagagg cacaaggtgt gcttgaagcc tggcgacgcg ctgatttggt cggaatggtt
     4681 ggttttaatt atcgatttaa tgcattttat aaagcagcca ggcaacatat cgaatccggt
     4741 aggctaggcg aacttgtttg tgttagatcc gtattttcaa ccgcgacgcg aacattaccc
     4801 acatggaaaa aagcccgaga aagggggggt ggcgctctgt tagatttggc ttcgcaccac
     4861 atcgacctcg tacgttttat ctctggtgaa gaaattcaag aggtatttgc acgtgtttgg
     4921 ttacagcaga gtgaagacga ctgtgcaatg cttcaactac ggctagctaa cgggcttcta
     4981 gtccagtctt ttttctcgtt aagcgcagta gaagaggatc gctttgaaat ctacggtatg
     5041 gagggaaagc tgacgatcga taggtatctt tcttttgatg tagaaattac cgatcctacc
     5101 gccgagtttg ccaggcttaa gtggcttggc cgcatgtttc aatcactgtt tcatagtcca
     5161 tatcttaaag agagaatctt ctcacctgga agtgagccat cctataaggc agcccttaat
     5221 cattttgtaa cagcggttcg aaaaaaacag ccaaccagtc cagatttttg ggatggctac
     5281 cagagcctcg ctgtaattga agcggcggag gaatcagcta gaatacaacg ggtagtttca
     5341 cttgcagatt ttgttaaaga agattttaat tatcaatact ctgagtagcc cacgcttaag
     5401 ctttatatgt ggcatcaatc tacttaacaa acccataaac gctataaagc aggaagaaag
     5461 caatgaaagc taataacagg ggcaacaaag gttcaaccga taaagatcgg ataatcgtaa
     5521 tcggattaga tgcctgtgac gctgatttag tagagcagtt gtcaaaagaa gggcgtatgc
     5581 cctttcttac ttccttgatg aaatcaggcc tctgggtccg actcacttcg acgaaggttt
     5641 tctttaatga ttctccctgg ccatcgttca atgccggggt ttctccagca aagcatggtt
     5701 actacaacta cctgcaaata aagcgaggca caactgaaat agaaagaata aatgctcatc
     5761 actgtcgtta ccttcctttc tggtccctcc tttgtgattc cggtaagcaa gtagctgtct
     5821 tcgacgttcc taaaactttt cccatcgaag gaatagatgg aatacaaatc tccgcctgga
     5881 gtgagcactc tttaaccctg aagcagccaa cctcaaatcc tcctgaattt atcagggata
     5941 taatcacaca ttacggaaaa tattcccatc ccagagagat cattacgccc agatcaacca
     6001 gacaggagag acgaatctac aaaacccttt tgtccaacct agaaaaaaag cttcagtcaa
     6061 ctgaatttct catgcgacag ggagattggg atctcttttt cactgtcttt tcagagggac
     6121 ataccgcagg gcatcaattc tatcaccgtt tcaacgaagg acactgggct tatgagccag
     6181 acgctcctga cgaaatccga aacgcactcc tacacatcta tactcagctt gatgcggctc
     6241 tcgccaggct gtttagccgt atctctgatc agacgacatt ttttattgtt tccgtgcatg
     6301 gcatggctat aaactattcc gcgaatcaca tgatgcctaa ggtaatggag aagctcggtt
     6361 ttcaggttcc tgctacaaag aaaaaacagt tgaacacgat aggatcttta ttgaaacgga
     6421 ctacgtttgc gcgaaatctt attccgctgt ccctaagaga ttttatcaac acgcatattg
     6481 tatctgagac tgttcacgac aaggcagtgt ctagtgcctt tagcagcggt atagattgga
     6541 aaaaaactaa ggcattcttt ttgccaagcg accacttcca gggatatata agcttaaatc
     6601 tcaaagggcg ggaacctttt ggcattgttg agcatggcgc agaatttgat gaactcagtg
     6661 acgagattcg ttatgaacta aaacatctga ctaacccgga gactggaaag cctgcggtac
     6721 atgatgttgt acgtacctct gacatatatc aaggacctaa cctgtatgag ctgcccgatc
     6781 tcgttgtcct atgggctgaa gatgcaccaa ttaatcaact ccaacatccc cgttttggtg
     6841 tgatctcagc aaaaggttat aaactgcgaa aggcacaaca tgtaacggac ggctttatga
     6901 ttgccggagg taaatatata aacaaaaacg catacttgag cggagccagc acctttgata
     6961 tagctcctac tattctctac cttatggaac aagtgatacc agatgagatg gatggaaagg
     7021 ttttgctcga actcatcgag gaagacttta aacggaataa cgaacccatc tatggaaaac
     7081 gaccagccat aatccctaaa gcgatgagga cgtaaggaaa aatgaaagcc ctgtctaaac
     7141 tgatgcgatg tgcgaccgct tcagcgtcag attaccgggt acgtttttca attgctggtc
     7201 ttgaaagaac ccttgcgaat cactatgctt ggctatggat ttcctgaggt gatattatgc
     7261 ttatccctaa ttttctcatc attggttcat caagatgtgg aaccacatca ctataccgtt
     7321 atctaaagca gcatcctcaa atttacatga gccctgttaa ggagccgtct tttttttcct
     7381 atgaaggtat gaaacctaac caccgcggcc ctagcgatga aaaattcaac cgcactgcgg
     7441 taacgaaaat cgaagactac ggggctctgt tcaaagacgt gactaccgaa accgctatcg
     7501 gggaagcttc gaccaattat atatacaatc caaaagcttc cgagcgtata aaccactaca
     7561 tacccaatgc aagactctta gccatccttc gtcatccggt tgagcgggca tactcatcgt
     7621 ttctatatct gattcgtgat ggacgcgaac cattaaggga tattgcacaa gcaattgaag
     7681 aagaaccaca acgcattcgc cagaattggg caccaatttg gaattataag cacatggggt
     7741 tttattacga tcagctcaaa cgctatttcg atcagtttga cagaagccag ataagagttt
     7801 atctctacga tgatttcagc actaatccga gcaggttgct acaagacatt tttcaattta
     7861 ttggagtagt tgatacattt gtcccagaca cttcgttgag acataatgtg tctggcattc
     7921 ctagaagcag ggtccttcac aaacttttga ctcaacaaag catgataaaa tactctgttg
     7981 aacggctcat cccagaagga tggcgtcgga gattaagcct gcaaaaacga aacctcatca
     8041 gaccgacgtt gccggcagac ttgcgtcggc aattgattga agaataccga gaggacattt
     8101 tgaagctcca agaacttatc cagcgagacc tttcaaaatg gctagagtga atagcggtac
     8161 cttttttata gggtgtcgag gatttacacc caccagcatt acaaaagcta taaggattcc
     8221 ctaagggcga aatatcccgc ctaatatctt actaacaaag tttatccatg aaaattctgc
     8281 tcgttaatga ttatgggacg ccaactggcg ggactgaact cctcacactg gcactcagag
     8341 acggacttag agagcgagga cacgatgccc ggctctttac cagtagtgca cgacccggaa
     8401 tatgggatag tcatgctgac tatgaatgca ttggtacgac ttcacgcttc cgcaccttac
     8461 ttcagacagc aaacccttgg gcttactggc agcttcgccg agtgttggcc gaatttcagc
     8521 ccgatgtggt gcatgttgga atattcttaa cccaactttc ccctttgatc ctccctttgc
     8581 tcaaggatgt gccgagcttg tatcatgccc actggtaccg accgatctgc ccgattggca
     8641 caaaaacatt gcctgacgga aacccctgcg aggtgcccgc aggagccgtc tgttaccata
     8701 accgatgcct gccgctgcgt gattggctgc cgctgatgct acaaatgaag ctctggaggc
     8761 gctggcggaa ttctttcaac cttattgtcg ctaacagtga agcggtaaag agtcggttag
     8821 ttgaagaggg cattgaaccg gtagaagtcg tttggccctc tattccgaca cgcccgctac
     8881 gtccacctct ttcaacacct caaactgtag ccttcgccgg tcggcttgtt cgtgaaaaag
     8941 gagtagacgt attaataaaa gcctttgcta ttgtagccaa gcaaattcct gtgtccaagc
     9001 tcctcatagc aggtgatggc ccggaatgga aaagtttgaa tatgcagatt aaaaacctgg
     9061 gattatcttc ccatgtcact atgctagctc atctatcgcg cccggaggta gagcgtcttt
     9121 tcgatggggc ttgggtgcag gtcgttccat ctaggtgggc ggaaccattt ggacttgtcg
     9181 ctgcagaagc tatgatgaga ggaacagctg tggtagctag cgcctccggt gggttaagtg
     9241 aaattgttca ggacggcgag actggttttc tcgtacaacc cggcaatatt ggtgcacttg
     9301 ccgaagcact cctgaagctt cttcaggatc gcgcgctgtc agaaaaggtg ggcaaggtcg
     9361 gacgcgaaat tgctctagca aaatttagtg aagcagctta cgttgatagg tttattcagt
     9421 tatatcagat cctctgccag agtaggagga aggcgaatga caattgatac aaaatactcg
     9481 ccattggtta ttctcggaat tgatgccgga gacccagtat ttattcaaca ttgggcgaag
     9541 gaaggttgca tgcctacagt cgcatcaatt atgaatcgag gttgctgggg ccaaactgcc
     9601 ggtccggaac tgttaagcga acacggcgta tggaaatctc tttttagtgg catctcacgc
     9661 agccagcacg gttactacta cttccgccag ttaaaacctg gcacctacga tctcaaaacc
     9721 gttactggag atgatattga agcatccccc ttctggtctt atctgcatgg gcaagataaa
     9781 aagattgcga taatagacgc acctgatggc cgtcccctgc ccggactacc cggaatccag
     9841 cttgcgaact gggctacaca taataacttg gatcctcacc gctttaccac gacttcggag
     9901 cctaccgagc ttctggaaga agtgagacgt aatttcgggc ccaagcttgt cacaatggaa
     9961 aatcatgaga gcaccttcga tgaagatagt aggatttatc gtcagttaat gaatcaggtt
    10021 gagaagaaag gcgcattgtg ccgccatctg ctcgcaagtg accgctttga tctgattgtc
    10081 attgtgttcg ccgagtcgca tgttgccaat catcagttct ggaaatacta catagaggct
    10141 aaggacggta agactactga acacaattta acccatgcca ttcgcaacgt ttacaaggga
    10201 attgacagag aaataggttt gctactaaaa caattgccta acaactccaa cgtcttcacc
    10261 gtgtcttcag taggtatgga agacgattat ccaactgcag gactcatcga agccttttgt
    10321 cggaaattag gctaccaggc gtcttctgaa tctaacggag gttcatttaa accgacgaat
    10381 cttgtgcgcc gcatggtgcc agagtcctgt cgtatagctc tcagtcggta tctacctaga
    10441 gagaaacggg aacgcttgtt ggctgagcag tttcgcagcg gaacaaactg gcgaaagact
    10501 acagcatttt ctatcccggc ctcttataca agctttatac gtgttaattt aaaaggacga
    10561 gagccagaag gtattgtaga acctggtgct gagtacatgg cattgttaca acgtatagaa
    10621 gcagacctca tgcaactcgt ggatcatcag actaatgagc tggctgtaac gattgttgcg
    10681 aagaccgttg agttgtttgg gtgtgaaccc catgtttcct tgcctgacct gtttgtagaa
    10741 tggaagcccg gccgatttat gcaacgcgtt gtgcatccca gagcagagtt ggtgcagaaa
    10801 aagccagact tttacaggcg tagcgaccac tcatctaaag gcttcgttgc cgctgcaggt
    10861 ccttatattc atatgcgagg agcgctaggt aatgttgagg tgctgaacct agctccaact
    10921 tttctttacc ttatggatga acctattccg cgggtcatga cgggtcaagt gataaagcag
    10981 attataatga ccgatgtgcc ctgacaaatt tagcataata aattagtcgt gtagtaatgt
    11041 ggtttcgaat tgcaagatca gcatacagat ggtttgtttc tgtagtaggt agatattacc
    11101 tcaagagaat gatagaggat ggattacccg aacgtttgaa gttgccgctt ctctttcttc
    11161 tgacaaaaag cctaagccaa gaagaccagt tgatcgtaag taggatcgaa tctgttcggt
    11221 ccaagatggc aaatcgtggc gatgtttatg tcgacattta tccttctccg agtcccggct
    11281 cagtgggcat ggtaacctca cctgaagtga agcccgcgcc tggtaaagtt aaaaaagttc
    11341 ccttgagtcg gattgcaaac acgacgagta tattaccata ctggggtacg tttctgtatc
    11401 tttgtgcaaa cgtcaatcga gcaacaacca ttttggaact gggaagttgt gctgggattt
    11461 caggttgtta tatggcttct ggtaaatact gcaaaagatt tattaccata gagggttctc
    11521 gtgccctagc cacccttgca gagtcaaaca tccgtcaagt agcagacaat ttcgcggtga
    11581 tcactgcctt gtttgacgat ggttttgatg aaactctgcc aaagctcaag gacgggctcg
    11641 acatggttta ttttgacggt caacatgaga aaatcgccac aatccactat cttgagcgct
    11701 taactcctca cttgaacgct ggttccattc ttatacttga tgacattcgc tggacttcag
    11761 atatgtggga agcatggcaa gctctttgca aatggaaagg catggcctgg acaatagacg
    11821 taggacgctt tggaatatgc gtttgggatg gggtggccac acaacccaag aattatgatt
    11881 tctccaaatt tacggattta tggaggaaga gaaagtctcg taactgataa gggtaaccta
    11941 atagattaat ctattggagc aatagctaac cgaaacagag attgaaacga tggccgcgat
    12001 taagtgtctg tgccgaggaa tttctacaaa gaacgaagaa ttaggataga ggaaactata
    12061 tgttaccaaa cttaatcata atcggcgctc aaaagtgtgg aacaaccaga ttgcattatt
    12121 acttgggtct tcatccgcaa atattcatgt cgaaggagaa agagttagaa ttcttcatag
    12181 aggagcgaaa ttggccaaat ggactggcat ggtatgcatc caattttaag ggaagggcta
    12241 aaatttatgg agaatcttct cccaactaca gctattaccc gcgtttcact ggcgttcctg
    12301 cacgaatgca tgctatagaa ccaaaggcta aattaattta catcgtccga gacccgattg
    12361 agcgaatgat ctctagttat atccaaaatt gtagtggtgg ccgtgaaaac aggtcgatag
    12421 aggaggcgct tcgaagtcca aataatcaat acagataccg tagcctatat tacatgcaat
    12481 taatgcaata cctagcgcac tatcctgcct ctcagatcgt tgtaattacc aatgaagacc
    12541 tggagtgcaa tctgccatcg actctccgta aggtattcga attcttagat gtggacccag
    12601 acttttgctc tcgctggtat gcattaaggc ggcacagcac tgcccgtaag aggcgaaaga
    12661 cgggaatcgg gatgcgcctt acgcaaacac cccctatgaa gttgctcagg cgtattccgc
    12721 tacgcctgcg gtggcctata gaagatcttc tctatttccc attttcacga aagattggac
    12781 gacctgtatt ggatgatagt ttaagacagg agttaattga ttatcttaga gatgacatta
    12841 atcgcctcag ggagtttacc ggcaataatt ttgaaaactg gtgtctagaa ttttaccgac
    12901 atatgaacat atagtaatga acaaccctgt agcaaactac aggtaattat caagttaaag
    12961 tagatgtgaa atctgtaaac ctggtgagga tatatcccac ctcgttttaa agacaattaa
    13021 aatggaagaa aaacaacctt tcttctctat aattattccc acttatggtc gcccaagtca
    13081 actagcagct tgtcttcagt ctattactcg tctccattat cccggtcata gcttcgaggt
    13141 catcgtggtg gatgatgaca gtaagatgcc gccggaggct gtggttgctt cttttcagga
    13201 ccagcttaac gtaacgcttc tcaagcaaat acacagcggc ccagcagccg cgcgcaacac
    13261 cggcgccgca cgagcaaaag gtgagttcct gaccttcatt gatgatgact gtgcacctgc
    13321 gtctaactgg ttgcaaaccc tagcagcacg ctttgctaag tcaccgaatt ctgcaattgg
    13381 tggacgtaca atcaatgcgc tccctaacaa cttgtattca acagccagcc agatgcttat
    13441 cgattacttg tacacatatt acaacgcaga ccacaatcag ggacgattct ttacttctaa
    13501 caaccttgct atgcctatag ttaagttcaa catgttgggg ggttttgaca cgggcatacc
    13561 ccgtgctgga ggagaggatc gcgacctgtg tgatcgctgg ctgcatcatg gctaccagat
    13621 gatttacgcc caagaagtct tggtctacca tgcacatgcg ttgacacttg acaccttctg
    13681 gcggcagcac ttcaactatg gccgcggcgc tttctacttc caccaggcgc acgcacgtcg
    13741 cggtcaggaa cggattaagg tggagcccct accgttctat ctgaatttgt tgcgttatcc
    13801 gttttcgccg gctcggggtc tacaaacgtt actgcttgca gtgttgcttg gtgtgtcaca
    13861 gatggcaaat gcaattggtt tcttatggga gagaatgctt caaacaaagg cgtctagatg
    13921 gcggatcctt tgatataaga aacttagaac cactcaaggc ccctagtctg ttgaagtaat
    13981 aaacgctttt agctatgctt aggccgcagg ctattaagtg acctaatttt aaataaccat
    14041 tacattacat tctacttaag aaggtaaagt acaatctcaa catgaaaacc gcagtaaatt
    14101 atcttttagg aagttggagc agaacgagta cctacatgct tagaacttgg tgggataccc
    14161 atggttcgat gctcatgcgt atagctatag cacttatggt caccgttgcg attgcatggt
    14221 taggatatca gttttggagg cttctctggc aacaagatcc tatatggcca acatccccta
    14281 gcggcgccgt tgatctaaac ctccgctaca aagaactaca taggtggttc gctggagagc
    14341 ctgttccacc agtctatccg ccagcaagct atataatctt atggcctctt ttaggctggc
    14401 tacccttcac gccggtaaag tggttctggg cagtcacaac tgtagcagcc ctcgtatggt
    14461 tagtcttttt ggttgttaaa gaaagcgggg ctaatacagc catagagcgg gttttcgttg
    14521 cattgatgcc tatatcaatg tatgcaacag gcgctaccat tggaaatggc cagctcatca
    14581 tacatatatt gcctgcgatc ctagccgggc ttttgattct acagaatagg aattgtacgt
    14641 tcggtaaaga acttctagct gtagcgctta taatattttc tctggttaaa ccaaacgtat
    14701 ctattccctt tctttgggtc gcgatcttag ctccaggcag attacgccct gctcttctcg
    14761 tagcgttagg ttacatagcg ctgacccttt ttgctctgtc tctcagagag acccccgaga
    14821 ttaatctctt gtcaccagaa aatatacaag aatcaccgca agatatacct aacataacaa
    14881 taccagaatc gtttgctgat ataacaaaat tatatacttg gagcgcaaca tgggccgaga
    14941 gggatagata ctctgaccta cctgtgctct tgacaactct cggcctgaag gcttggatca
    15001 ttcctgtcac gctgtttata atggcattgc aggggctatg gaccttccga tatcgtaatg
    15061 gtgatatctg gcttctcgtt agtgttgctg cgattatagc ccgggtctgg gcgtatcatc
    15121 gctggttcga tgaccttctt atccttctac ccatggtagc cttgtttcgt atcgctaata
    15181 gggagcggtc aactaacagt cgtaaggttg ttgctggtgt actactggca attacacttc
    15241 ttagtacgct agcacctgga ggtttgtatc tcttcccacc gccgtggaat tcgctttacg
    15301 cggtggggca gtcagtaata tgggtaatcg tgttgatttt cctccttgac caagcaaggt
    15361 gtgagaagaa ggcataatat ttattgtttt taactaaagg ttggtataca aagaaatcgg
    15421 gttttccaag tgtgccaaat agcttgagtt ttataggggt tttgattcac tttattactc
    15481 tctaacgctc ttagggtgat acttgaatat agaagggaag aaatagctga cgctcgtttg
    15541 ctggtaattt acagtaataa aaacatttac gagaaactgt ctagcaacaa tataaaaaca
    15601 ttctatctca tacacaatag atattggtca ggaaatttta agagtttatt aaaagatgtg
    15661 atgaaggatc taagatttta acttatggac actcagtctt ttaaaagtct tttaaaggat
    15721 tagaaactta taaatttatg ttagtataga aagaatatgt atgagtaaga tttcttctta
    15781 taaataaaag aactattggt tgaattgtta ggtttattaa taaagtacag cacatggagt
    15841 atcaaccttt tatatcagtt atcatcccag tatacaacgg ctgtaaatat ctgaatcggt
    15901 gcttagatgc cttaatgaca tcatcttatc gacaattcga gatcatcgta gtggatgatg
    15961 cctctactga tgacagcgtt gatatctgcc ggaaaaaagg aatagctgtt gttcagatgc
    16021 cgcaccaatc cggtcccgct gccgcacgta attatggtgc ccaaaaagca cgaggagacg
    16081 tattactatt tatcgactca gatgtgttag ttcagcaaga aactattact ctagttgcag
    16141 ccgattttat caataatcct gatatagtgg ccatatttgg ctcatacgat gatagcccag
    16201 atgcttcaga ttttttgtct cagtttcgaa acctttttca tcattttatc caccagaatt
    16261 ctcaccaaga agcgaaaaca ttctggacgg gatgcggtgc aatatataag gaaatatttt
    16321 atcaattaaa gggttttgac gaagactggt tctcaataga agatatagaa ctaggccacc
    16381 ggatttggga taatgggtat cgaatcatgc tggacaaaga aatacaggtt aagcacctta
    16441 aacaatgggg gttgaaaaat tggttgaaaa cagacatatt ataccgtgca gttccctggt
    16501 cgcaattaat tctctcaagc ggctacatgc catgggatct gaatttacaa acacctcata
    16561 taataagcgc agttctagtt ggcttgttaa cccttacgac ttttcttatt tttttgaatc
    16621 ccataaatct cttcggtgtg catttaaatg ttattttatt aatactctca tttcttctaa
    16681 tcttcaccat aataattctt aatcgaggct tatacggctt ttttttacag aagagaggga
    16741 taaaattcac aattctcgct atcccaatac atttcatata ttatttttat agtggtgtag
    16801 cttttactct cagctggact cttaataagc ttgacaatct aattcccggc ttcaggaaat
    16861 ttaatcgctc acataaaaga aaagcataat atattcttct actgtttcgt ctcatctctt
    16921 tgaaatcgaa cgtgcaataa catacatatt ttaaatagat ttgtatatca aattaagtgg
    16981 ttagagggta aataatgggt ctcctagtaa tgaaattcgc taattctagc acctcgagac
    17041 ttcgaatctg gtggcataag cacgcttcta tgctactaag cttcgcagtc gctcttatgt
    17101 ctgtatttgc gttgatgaag ctaggagagg agttctggcg gcttactttg gactcgagca
    17161 gtattggcgc tattgatct
//
LOCUS       DATDRL010000368         2719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3597376, whole genome shotgun sequence.
ACCESSION   DATDRL010000368 DATDRL010000000
VERSION     DATDRL010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3597376"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..426
                     /locus_tag="VI935_12245"
     CDS             196..426
                     /locus_tag="VI935_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26399.1"
                     /translation="MTTQNYYKGVQHTVYISTSMGVGCEHCRTQIAADRFAESTNHYI
                     EQHGYKLLHVGAETSRDMDGKPWHSTVAVLGK"
     gene            550..2091
                     /locus_tag="VI935_12250"
     CDS             550..2091
                     /locus_tag="VI935_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015205596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HLE26400.1"
                     /translation="MSGEETNQPINSTPIIRADGITDSERYLKRLCDRTFLSLWSYPG
                     IYRDQGLQQRKEGKEVCDLLVVFEDHIIIFSDKDCKFPDSGKITIDWNRWFRRALQKS
                     ANQVWGAERWIRAYPDRLFLDRDCKKQFPLELPDMEKAKFHLIVVAHDASQRCKKELG
                     GSGSLMVHSPIKGEAHYVTEGAIGTPFAIGDLNPYETFAHVFDDTTLDIVMNTVDTIS
                     DFVNYLTCKERFLRSGRTIIATGEEDLLAFYLKNINDEGVHDFVVPSNVSAVFLDEGH
                     WDIFSRSPERQAQIAANEISYVWDRLIETFIKHALAGTHYFATHPDIKYSEKIYRFIA
                     REPRIRRRLLARSLVGLIEKTPPTLRATRIMMPSHKGDPYYVFLLLSQPNNIPYTEYR
                     EGRVNLLVSYCKVTKLKFPEASDIIGIATEPGNELHRSEDVLYFDVREWTEEDQFDAK
                     SLQEEFGLLNKLTMLDGWEKEYPDIDYQAEQAPARPAFKLPHNPRNKPCPCGSGKKYK
                     RCHGR"
     gene            complement(2372..>2719)
                     /locus_tag="VI935_12255"
     CDS             complement(2372..>2719)
                     /locus_tag="VI935_12255"
                     /inference="COORDINATES: protein motif:HMM:NF014640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HLE26401.1"
                     /translation="EKSKLIQELNDLRERTVFGLKRLVEFYAKDISRIQSELGRFIRI
                     KLDSSILELRALSGKLDALSPLNVLERGYSITYKLPLMTVIKESKELKTGDKVSVRFH
                     EGKARCLVEDIED"
BASE COUNT          830 a          569 c          599 g          721 t
ORIGIN      
        1 gtatccacat taaaaaaatg ggaaaccaat aaccgtgtgc ccgaaggccc gaccaaggct
       61 tatctgacgg tcattgagaa aaacccgcat gcggtaaaaa aagccctgca cagcgctgtg
      121 tgataacaga cggtatggaa acttaaattt gtaggtaaaa taaatagtta tgtataaagt
      181 aaggagcaaa ttaaaatgac aacgcaaaat tactataagg gagttcagca tactgtttat
      241 ataagcacga gtatgggggt tggctgcgaa cactgtcgta cccaaatcgc cgctgatcga
      301 tttgctgaat ctactaacca ctacattgaa cagcacggtt acaaacttct acacgtaggc
      361 gcagaaacga gccgtgatat ggatggtaag ccgtggcata gcacggttgc ggtgcttggg
      421 aaataagaaa gataaaaatt ctgacatcta atacggaata caaaatcttt aagtgaaaaa
      481 cagacaaaaa tttccacata ttgagttgtt agacatagat tgagatagag gaagaaaaag
      541 acaatttatt tgagtgggga agagactaac caacctatta atagcacacc tatcatccgt
      601 gctgacggta tcacggactc agagcgttat ctaaagcgcc tgtgtgatcg aactttcttg
      661 tccctctgga gttacccagg catttatcgc gatcaaggtc ttcagcagag gaaagaggga
      721 aaagaggtat gcgacctgct cgtcgttttt gaagaccata tcattatttt ttctgataag
      781 gactgtaaat ttccggacag tgggaaaata actatcgatt ggaaccgatg gttcagacga
      841 gccctccaga agtctgcaaa tcaggtttgg ggagctgaaa gatggattag ggcttaccct
      901 gatcgccttt tcttggaccg ggattgtaaa aaacagtttc cactggaatt gccggacatg
      961 gaaaaggcaa aattccatct tatagtcgtt gctcatgacg cttcacaacg atgtaagaaa
     1021 gaacttggcg gcagcgggag cttgatggtc cactcgccaa tcaaagggga ggctcattat
     1081 gtcactgagg gtgctatagg gacgcctttc gcaatcggag accttaaccc ttatgaaacc
     1141 tttgcccacg tatttgatga cacaacgttg gacatcgtta tgaataccgt agatacaatt
     1201 tcggacttcg ttaactatct gacatgtaaa gagagatttt tgcgctctgg aagaacaatc
     1261 attgctacgg gcgaagaaga cctgctggcg ttctatttga aaaacatcaa tgatgaaggc
     1321 gtgcatgatt ttgttgtacc ctctaacgtt agtgctgtat tcttggatga aggtcactgg
     1381 gatatttttt ctcgaagtcc agaacgtcaa gctcaaatcg ctgctaatga aatcagctat
     1441 gtatgggaca gattgattga aactttcatc aagcacgcgc ttgcaggcac ccactatttt
     1501 gctacccatc ccgacattaa atattcagag aagatttacc gcttcatagc gagagagccg
     1561 cgcatcaggc ggcgattact ggcccgctca ctagttggac tgattgaaaa aacgcctcca
     1621 acattacgtg caacgcgtat aatgatgcct tcccataagg gcgacccgta ctacgttttt
     1681 cttcttcttt cgcaacctaa taatattcct tataccgagt atcgtgaagg cagagttaac
     1741 ctcctagtgt cctattgcaa agtcaccaaa ctaaaatttc cagaagcttc agatataatt
     1801 ggaatcgcaa ccgagccagg aaacgagctt cataggtcag aggatgtgct gtattttgat
     1861 gtgagagaat ggactgaaga agaccaattc gatgccaaaa gtctacaaga ggaatttggc
     1921 ctattgaata agctgacaat gctcgacggg tgggagaagg aataccccga tatagattat
     1981 caggcagaac aagcaccagc acgtcctgca ttcaagctgc ctcataatcc acgtaacaaa
     2041 ccctgtccgt gcggaagtgg caaaaagtac aagcgctgtc atggaaggtg atagtattaa
     2101 gggccgaaaa agctaaaaat acagcaggct gcaaggtgtt gggtagttta tttaaatcta
     2161 cactgaaaca ctattaccaa ttctgagtaa aattccgtcg atcgacagcg ttgcataagg
     2221 taacctttta gctacaaaga cactaggaca cgaagttaga atacagcaaa aatgagtttt
     2281 cttctggctt ccgaactctg tattctgtct cctgaatctg ccctactcat atccctcttt
     2341 gtgccttggt gtcttagtgg caagataacc tctaatcttc aatatcctca actaaacacc
     2401 ttgcctttcc ttcatgaaac cttacagaaa ccttgtcacc ggtttttaac tctttagact
     2461 ccttgataac tgtcattaaa gggagtttat atgtgatgct gtatccacgt tctaggacat
     2521 taagcgggct gagggcatcc aatttacctg agagtgctct gagttctaat attgatgaat
     2581 cgagcttaat tcgaatgaat cttccaagct cagattgaat gcgggaaatg tccttagcgt
     2641 aaaattcaac cagccttttt agtccgaaaa cggtacgttc tcttagatca ttaagttctt
     2701 gaatgagctt actcttttc
//
LOCUS       DATDRL010000369         5862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2540956, whole genome shotgun sequence.
ACCESSION   DATDRL010000369 DATDRL010000000
VERSION     DATDRL010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2540956"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VI935_12260"
     CDS             <1..284
                     /locus_tag="VI935_12260"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF024684.4"
                     /note="catalyzes the NADP-dependent rearrangement and
                     reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to
                     2-C-methyl-D-erythritol 4-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HLE26402.1"
                     /translation="ELSFEDADLGKFPVLSLAYDALEEGGTMPAVMNAANEMAVQHFL
                     KGDLKFLNIIKVVKQVMNLHKTHTGGSLDEILESDNWARSTAESIINRM"
     gene            505..2103
                     /gene="rseP"
                     /locus_tag="VI935_12265"
     CDS             505..2103
                     /gene="rseP"
                     /locus_tag="VI935_12265"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HLE26403.1"
                     /translation="MTVILAFLFVIGILVFIHELGHFILAKWSGVKVEKFSLGFGKKI
                     LGFRRGETEYLISLLPLGGYVKMYGEGGEGGFIIENLEPSSPAEKVGFKSGDKIISID
                     EINLTAYSRWKELEFTLGKDLEREYTFVIERDEKKLKLKVKPEGLNGIKAFSEKDYPR
                     SFSNQSILNRFKIVFAGPFMNLALPFLLLPIVFMLGIGVPAYLETPPVIGYVEPDSSA
                     SQAGFQKGDKILTIDGENINTWRDANIAFQSNPGALLKVKVERGGEMKIFEFKASPSP
                     EGIVEIGIREPLEAKVGSVIDGTPAERAGLKKGDKVVAINGIIVSDWYHMKSIIENKT
                     NQEITLLIERGSSRLEVKVTPEPMQGGSQGAIGITQYIEEIQKKYGFFESIVEGIKEA
                     AKLVVEITVLLLSFLYKLITGKLSLSAAGKSIAGPILIAKVSGSAAEGGLATLLQFTA
                     FISINLGLINLFPIPMLDGGHILYLSIESIRRRPLSQRTLEICQRVGFTFLIFIMFLA
                     IYNDLSKLKGSIIESIGRLLEAFR"
     gene            2313..3215
                     /locus_tag="VI935_12270"
     CDS             2313..3215
                     /locus_tag="VI935_12270"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HLE26404.1"
                     /translation="MNLLKLGKDNWARTRPTKEGIIFLILTLFVGFAALNTGNNLLYL
                     VFGMMLSFVAASGIMSMINLSGIEVKTIAPEDVFALTPIPFRFSVRNLKPLIPSYSLT
                     IDIEGKSTFITYLPPKSTKNVTINNFFKKRGWNKIPKAILFTRFPFGFFKKWIKINED
                     DEVILVYPKVENAHMELNEFQEKFGELETEAIGFGSDLRSIRTFNQGANPKFIHWKTT
                     AKTGRLMYRELQDDKSKRYIIEFTPTKDENHLEFEITRAASVLLELLQHGYDVEFIAP
                     DRTFTSSQIGRFPRPVLRYLALYN"
     gene            complement(3378..3752)
                     /locus_tag="VI935_12275"
     CDS             complement(3378..3752)
                     /locus_tag="VI935_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26405.1"
                     /translation="MSKEADNFLRAEQSAQDLVEALTQLKGETVSYKTSTKELDAVRQ
                     KLVELIDSVQAVAKDSHEVIHILKEIGGPEILRSTDGLSKKLDELSFKHSQQLQRLRV
                     FVIVALSTSVLSLIGIVMLLLR"
     gene            complement(3765..>5862)
                     /locus_tag="VI935_12280"
     CDS             complement(3765..>5862)
                     /locus_tag="VI935_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HLE26406.1"
                     /translation="QLASFLLPGRTVVIDPIIALMDDQIDNLQSVGIDRAIAITSQIN
                     DPLEKERVLSLFGQGEYLFAYIAPERLQTVEFRKSLSGLTTHTPISLIAVDEAHCISE
                     WGHDFRTAYLNIGRTSRTYCELSGVMPPLLALTGTASRAVLKDVQRELQIEDFDATIT
                     PKSFDRPELKFHVVTSSSAEKVSRLLGYLGQTLPSRFTMPPASFFQTHGKATYSGLVF
                     CPHVNGEYGVVEVSEKIRASLSIPLGIYSGKEPKFYSEHDWKSAKQRVTRQFKHNQIP
                     LLVSTKAFGMGIDKPNIRYTVHYGIPPSIESFYQEAGRAGRDRKTAYCCIIVSVDDAE
                     RAKHLLDPNTKPEEVLSTVKNLSWDDNDDVTRALFFQTNAFPGIAVEKSRVAQVIRSL
                     PDLSTRAARSIKFSDLARPEAEKALHRLVVLGIISDYTINYNTDEFTVKLAGATTDSI
                     IETYGRYVAGYLGARRQAEIDKASKLTNLPLTGFAFEMVDLLLHFIYDVIERGRRRAL
                     SEMLLASTATPSDSAIRQRILRYLEATEYSEALEAILSQSDAGLTKTKKIVEAIRSPN
                     EAAELRGQVARYLESYPDHPGLLMLRSLAEAFSRDKNATIVQQNFSASISSASENYSV
                     DEAELFNFIVWSITKVADRNLILAMELQADMLRQYQNRRFARILIEQSPIELASTPAW
                     FLLDNLTKRSKEVLSI"
BASE COUNT         1746 a         1174 c         1320 g         1622 t
ORIGIN      
        1 gtgagctttc ttttgaagat gcagatcttg gaaagtttcc tgtgctttcc ctcgcttatg
       61 acgctttgga ggaaggtggg acaatgcctg cagtaatgaa tgccgcaaat gaaatggctg
      121 ttcaacattt tcttaaaggg gatttaaaat ttttaaacat tataaaagta gttaagcaag
      181 taatgaattt acataaaacc cacactggag gttcacttga tgagatactt gaaagcgata
      241 actgggcgag aagtacagcg gagtcaatta taaataggat gtaaccattt agccacaaaa
      301 tcagatgcgg caggttgggt aattcggcgt agccaattaa cactaaggat caggtatgaa
      361 aataaacacg atacaggata ttcgatgcgg gatacaggat ggatataact ttcaagtatc
      421 atgcatcacg tatcttgtat catttattcg cgtaaattct tgtttatctg ttattaaaca
      481 cttgcaacgg gagaagaaaa aacaatgact gttatattag cgtttctttt tgttattgga
      541 attttagtat tcatacatga acttggccac ttcatcttgg caaaatggag cggggtaaag
      601 gtagagaaat tctctctagg gtttggaaag aaaatcctcg ggttcaggcg tggtgaaaca
      661 gaatacctta tctcattact cccccttggc ggctatgtaa aaatgtatgg tgaaggagga
      721 gaaggaggtt tcattattga gaatttagaa cctagttccc ctgctgaaaa ggttggattc
      781 aaatcaggag acaaaataat aagtatagat gagataaact taaccgcata ttctagatgg
      841 aaggaacttg agtttacact aggtaaggat cttgaacgtg aatacacatt tgtcatagaa
      901 agagacgaaa aaaaattgaa actcaaggtc aaaccagaag gcttaaatgg tataaaagcc
      961 ttctcagaga aagattatcc ccggagcttt tctaaccagt caattctaaa caggtttaag
     1021 atagtctttg cgggaccctt tatgaatctt gcgcttccat ttctgcttct tcctatagtt
     1081 ttcatgcttg gcattggtgt gccagcttac cttgaaaccc cccctgtaat cggttacgtt
     1141 gagccggatt catccgcaag ccaagcagga tttcaaaagg gagataaaat actaacaata
     1201 gacggcgaga atataaacac ttggagagat gctaatattg catttcaatc aaacccgggt
     1261 gcccttctca aggtaaaggt agaaagaggc ggagagatga aaatcttcga gtttaaggct
     1321 tccccctctc ctgaaggaat tgtagaaata gggattagag aaccgcttga agctaaggtt
     1381 ggaagtgtaa tagatggaac cccagcagaa agagcaggac tcaaaaaggg ggataaggtt
     1441 gtcgcaataa acggcataat agtttcagat tggtaccata tgaaatccat tattgaaaat
     1501 aaaactaatc aagagatcac tctccttatt gagagaggta gcagtcggtt agaggtcaaa
     1561 gtgaccccag aacctatgca gggaggcagt cagggggcaa taggcattac ccaatatata
     1621 gaagagattc aaaagaagta cggttttttt gagtcaattg ttgagggaat taaagaagcg
     1681 gcaaaactcg tagtcgagat taccgttctc ctactttcat ttctctacaa gcttatcaca
     1741 ggaaaattgt cactgagtgc tgcgggaaaa agcattgccg gccctatcct tatcgctaag
     1801 gtttcggggt ctgccgcaga gggtggacta gcaacacttc ttcaatttac tgcctttatc
     1861 agtataaacc taggtcttat aaatctcttt ccaattccta tgcttgacgg agggcacatt
     1921 ctctacctct caatagaatc aataaggaga cgacccctta gtcaaagaac actcgaaatc
     1981 tgccagcgtg taggttttac cttcttaatc ttcatcatgt ttctcgcaat ttataatgac
     2041 ctttctaaac taaagggctc gattattgaa tccattggga gactactcga ggcttttagg
     2101 tagctatcta agtgcaaatt cttaggataa agaataaaca atactcaagg atgtagacaa
     2161 gaagtaaggt agttttatct taaccatttc catacaaaaa cgtgggataa aatctatatc
     2221 aatatttgta ggggcgaacc tcgtgttcgc ccagaatgaa acaatcatta tataaatggg
     2281 caatcacaag gattgcccct acaatataaa atatgaatct gcttaaactc ggtaaagaca
     2341 attgggctag aacacgacct acaaaggaag gaattatctt tctaatactt accttattcg
     2401 ttggttttgc ggctttaaac acagggaata atctacttta cctcgttttt ggtatgatgc
     2461 tgagcttcgt agcggcttct ggaattatgt ccatgattaa tctttcagga atcgaggtaa
     2521 aaacaattgc tcctgaagac gttttcgcat taactccgat acccttcaga ttttcagtta
     2581 gaaaccttaa accccttata ccatcctatt cactgacaat tgatatagaa ggaaaaagca
     2641 catttataac ttaccttcca ccaaaatcta caaaaaacgt tacaataaat aactttttca
     2701 aaaaacgggg atggaacaaa attcccaaag ctatattatt tacaagattt ccctttggct
     2761 tctttaagaa atggataaag atcaacgaag atgatgaggt gattctagtt tatccaaagg
     2821 ttgaaaatgc acatatggaa ttaaacgaat ttcaagagaa gtttggtgaa ttagaaactg
     2881 aagcaatagg gtttggaagt gacctaagat caatcagaac ctttaatcaa ggagccaacc
     2941 ccaaatttat tcactggaaa accacagcca agacgggaag gttaatgtac agagaacttc
     3001 aagatgacaa atcaaaaaga tatataattg agtttacacc cacaaaagac gaaaaccatc
     3061 ttgagtttga aataacacgt gcagcatcag tcctattaga gttactccaa cacggctatg
     3121 atgtcgaatt catagcgccg gatagaacat ttacctcatc acaaattggc aggttcccaa
     3181 gacccgtgct cagatacctt gcgctttata attaaaacga attgctctta tctataagag
     3241 cactgaaatg acgagtctta gtaagataaa atagatttaa gtccgtggga agttaacgac
     3301 caatcttacc agcggctatg gagcgcagag gaataaccgt ccggtgcagc gctttgttag
     3361 ctgcagttac cgtttctcta tcgtaataac aacatgacaa ttccgatcag cgaaagaaca
     3421 gatgttgaga gcgcaacgat tacgaagacc ctcaatcgtt gtaattgctg agagtgttta
     3481 aaagacagtt catctaattt cttggagagt ccatcagttg agcgtaggat ttctgggcct
     3541 ccaatttctt ttagtatgtg aataacctcg tggctatcct tagccaccgc ctgaactgaa
     3601 tcaatgagct cgaccagttt ctgtcggacg gcgtcaagtt cttttgtgct ggtcttgtat
     3661 gaaacggtct cgcccttaag ttgcgtgagt gcttcgacca agtcttgagc agattgctcg
     3721 gcccgcagga agttgtcggc ttcttttgac attagttgac ctccctatat tgacaagact
     3781 tctttcgaac gcttggttag attatccagc aggaaccaag ccggagttga cgcaagttca
     3841 attggcgact gttcgatgag tatgcgggca aagcgccgat tctgatattg acgtaacata
     3901 tcggcctgta actccattgc gaggatcagg tttcggtctg ccactttcgt aatagaccaa
     3961 acgatgaaat tgaacagctc agcctcatcc acgctgtaat tctctgaagc ggatgaaatg
     4021 gaagcagaga agttttgctg aacaatggtt gcgttcttat ctcttgaaaa tgcctctgct
     4081 aaagaccgca acataagaag gcccgggtgg tctggatagg attcaagata acgagcgact
     4141 tgtcctctca gctccgcagc ctcattagga gagcgaatgg cttcgacaat cttttttgtc
     4201 ttcgtaagcc cggcgtctga ttgggaaaga atggcctcga gtgcttcaga atactcagtc
     4261 gcttcaaggt agcgaaggat ccgttggcgt atggccgaat cactgggtgt tgcagttgaa
     4321 gcaagtagca tttcagacaa tgcacgtctc ctgcctcttt ctatgacgtc gtaaatgaaa
     4381 tgtagaagaa gatcgaccat ttcgaaagcg aaccctgtca atgggagatt ggtcaacttg
     4441 cttgccttgt cgatttcggc ctgtcgtctt gcccctaaat aaccagcgac ataccttcca
     4501 taagtctcga tgatagaatc cgtagtggct ccggccagct ttactgtgaa ttcatctgta
     4561 ttgtagttga tcgtgtagtc agagatgatt ccgagaacca ctaaacggtg aagtgccttt
     4621 tctgcttcag ggcgagcaag gtcggaaaat ttaattgacc gtgcagccct cgtggataag
     4681 tctggaagtg agcgaatgac ctgggcgact cgtgactttt cgacggcgat tcccgggaag
     4741 gcattggttt ggaaaaatag agcccttgta acgtcgtcgt tgtcatccca gcttaggttt
     4801 ttaacggttg ataacacctc ctcgggtttc gtgttgggat cgagtaaatg tttagctctt
     4861 tcagcgtcgt ccactgagac gataatgcaa cagtacgcgg tctttcgatc tctgccggct
     4921 cttccagctt cttgatagaa agactcaata gaaggtggta tcccatagtg aactgtatag
     4981 cggatgttag gcttgtctat gcccatcccg aaagctttgg ttgaaaccag caatggaatt
     5041 tgattatgct tgaattgacg tgtgaccctc tgctttgctg atttccaatc atgctcggaa
     5101 taaaacttag gctcctttcc actgtagata ccgagaggga tactgagtga agccctgatc
     5161 ttttcggaca cctcaacaac cccatattca ccgttgacgt gcggacaaaa aaccaagccg
     5221 gagtatgttg cctttccatg tgtttgaaag aaagatgccg gcggcattgt gaatctgctg
     5281 ggtagcgtct ggccaagata gccaagaagg cgtgaaacct tctctgcgga tgatgaagtg
     5341 accacgtgga atttaagttc gggacggtcg aaagactttg gagtgatggt tgcgtcaaaa
     5401 tcctcaattt ggagttcacg ttgcacatct ttgaggacgg ctcgcgatgc ggttcctgta
     5461 agagccaaaa gcggcggcat gactccactc aattcacagt aggtgcgact tgtgcggcca
     5521 atgtttagat aagcggtacg gaaatcgtga ccccattcag agatgcaatg ggcttcgtcg
     5581 accgcaatca acgagattgg cgtatgggtt gtaagcccgc taagtgattt cctgaattct
     5641 actgtttgta atctttctgg tgcaatgtat gcaaacagat actccccctg accaaaaaga
     5701 gaaagcactc tttctttctc tagtggatcg ttgatttgac ttgttattgc gattgcacga
     5761 tcaatgccga ctgattgaag attgtctatc tggtcgtcca tcaatgcgat gattggatca
     5821 atgactactg ttcgccctgg aagaaggaaa gaggcaagtt ga
//
LOCUS       DATDRL010000370         4123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3519570, whole genome shotgun sequence.
ACCESSION   DATDRL010000370 DATDRL010000000
VERSION     DATDRL010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3519570"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1371
                     /locus_tag="VI935_12285"
     CDS             163..1371
                     /locus_tag="VI935_12285"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HLE26407.1"
                     /translation="MGDIRKIILAYSGGLDTSVILRWLIERYKAEVIAFVADLGQGED
                     LEEIKEKAIKTGASKVYVEDLKDEFVRDFVFTALRANAVYEGMYLMGTSLARPLIAKK
                     QIEIAKKENADAVSHGATGKGNDQVRFELTYYALNPAIGVIAPWREWDFKSRKDLITY
                     AKSHGIPVPVTKEKPYSCDRNLFHTSYEGGILEDPWAEPPENMFEMTVSPDKAPNEPS
                     YIDIDFEKGVPVRVDREELSPVELLHKLNKIAGKNGVGRVDMVENRFVGIKSRGVYET
                     PGGTILHAAHRAVESITMDREVMHLRDMLTPKFSELIYYGFWYAPEMEMLKAAINESQ
                     QNVSGTARMKIYKGAVSVAGRRSPFSLYQKDLATFEEERVYNQRDATGFIRINALRLS
                     IKKLLHKVKR"
     gene            1867..2874
                     /gene="queA"
                     /locus_tag="VI935_12290"
     CDS             1867..2874
                     /gene="queA"
                     /locus_tag="VI935_12290"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HLE26408.1"
                     /translation="MKTADFNYNLPHELIAFYPVSNREESRLMVVYRNNQKIEHRRFL
                     ESPQILKEGDILVLNNTKVIPSRLFGKKDKGQQVEVLLTERISPCFWKAIMKNPKHDL
                     MIEFDGGLKGKVLKDGKDEWIIEFEEGADDYIEHSGRMPLPPYIKRMPEENDRIFYQT
                     VYADKKGAVAAPTAGLHFTRRLLDEIRQKSVEILYITLHVGAGTFKPVKTENVEEHKV
                     HQEYREISNEAALAINRAKREGKRIVAVGTTVVRTLEGALDKNGDVQPALGYTDLFIY
                     PGFKFQIVDVLITNFHLPRSTLLMLVSAFTGRELILKAYKEAIEKGYRFLSYGDAMII
                     I"
     gene            2898..>4123
                     /locus_tag="VI935_12295"
     CDS             2898..>4123
                     /locus_tag="VI935_12295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HLE26409.1"
                     /translation="MQVEIEKLTFGGAGMARVNGKVVFVKGGLPGDVLKVKITKDKGS
                     YAEAIIEEVLRTSPERTQAPCPVFGECGGCQLQHLKYPSQLIAKENILRETLERIGGL
                     RGIEIEPIIPSLEEYRYRNRVTLSIWFQKGRYRLGFYEEGSHKRVPIEGCPIASSIIN
                     EAISRLDKFLSSIDNPRFPLVRIHIASDGTSAHISLVPSSEKDPKRLNGLRDHIKKSL
                     EAGSVSVAGYNEEEFEFNQSGLKFYSSPSVFIQSNREINERLVETLVEWSDLKGHERA
                     LDLYCGVGNFLLHLAKRAKEVVGVDVSAKAIKLAKKGAEANSITNVNFDCSPSELYAE
                     ESLIRGDKFDLVILDPPREGAKGILKALGLLSPRKIIYVSCDPPTLARDLKTLTEIGY
                     KLIKIRPYDMFPETYH"
BASE COUNT         1268 a          743 c          986 g         1126 t
ORIGIN      
        1 gcaacattct atttttatga ccttagggga agtcaactgg ataaacagat gagcaaatga
       61 aatctttatt tgtttagact acctgatttt aaaagtctaa agatttgctt acacctttgc
      121 ctttagcttt cttcaaaatg aattcttgga ggctttttga aaatgggaga tattaggaaa
      181 ataatactcg cttactctgg cggccttgat acatccgtga tactcagatg gcttatagaa
      241 cgatacaaag cagaggttat tgcgtttgtt gctgatctag gtcaggggga ggatttagag
      301 gagataaaag aaaaggctat aaaaacaggc gccagtaaag tgtatgtaga ggatttgaaa
      361 gatgaatttg taagggattt tgtttttaca gcccttcgcg cgaacgcagt ttatgaaggg
      421 atgtatctaa tggggacctc tcttgcacga cctttgattg ccaagaagca gattgaaatt
      481 gcaaaaaaag aaaatgcgga tgcggtatca catggcgcaa caggaaaggg caacgatcaa
      541 gtaaggtttg aattaaccta ctatgcgctt aacccagcta ttggcgttat agctccgtgg
      601 cgtgagtggg attttaaatc gagaaaggat cttattacct atgcaaaaag tcatggaatt
      661 cccgttcccg taactaaaga aaaaccctat agctgcgata ggaacctgtt tcatacaagc
      721 tacgagggtg gaatacttga agacccttgg gctgaaccgc ctgaaaatat gtttgagatg
      781 acagtttccc ctgataaagc cccaaacgag ccttcctaca ttgacataga ttttgaaaag
      841 ggtgtgccgg taagggttga ccgggaggaa ctttctccag tagaactact tcataaacta
      901 aacaaaattg ctggaaaaaa cggcgttgga agagtagaca tggtggaaaa tagattcgtt
      961 gggataaaat cacgaggtgt ttacgagacc ccgggtggta caattcttca tgctgcacac
     1021 agggcagttg agtctattac aatggacaga gaggtcatgc atctaagaga catgcttact
     1081 cctaaatttt ctgagctaat ctattatggg ttttggtatg cacctgagat ggaaatgctt
     1141 aaggctgcta taaatgagtc tcaacagaat gtgtctggaa cggctagaat gaagatttat
     1201 aaaggagctg tatcagttgc aggcaggcgt tctccatttt ctttatatca gaaggatttg
     1261 gctaccttcg aggaagaaag ggtctataat cagagggatg caacagggtt tataaggata
     1321 aatgcattaa gactttcgat aaagaaactt ttacataaag ttaaaaggta agtaataaag
     1381 ggcgaaccta tgtgttcgcc ctttattaca caggccgaat caaatacacg agatattatt
     1441 ggtagggcga cccttgcctg gggcgagccc ggtcgagccg cggacaccaa aataatcgtg
     1501 caagcgctag gtctgctcgt tacccattgg taacttgata aatttcaact tgttaatacc
     1561 tcgataggca gggttttcta accctgccag ccaagacaag gaagtctcgg ctatcgtcgt
     1621 tttactcggt gggcaaccta actgtcccct cgacttactc ggggcaggct tgctagtggg
     1681 cgaggaggtc aggcaactgt aatggcaaaa taccagagag ttataccaac acacaattag
     1741 acgaattcaa ccttatacca aatcatgttt aatgtagggc aggtttcaaa cctgcttctc
     1801 ctcctacagt aatcatttgt tttccttaaa gacacactca tggtctcttc tggtagaata
     1861 aatcagatga aaacagccga ttttaattat aatctccctc atgaactgat cgcattctat
     1921 ccagtttcta atagggagga atcaaggttg atggttgttt atagaaataa tcaaaaaatc
     1981 gaacacagaa gatttcttga atcacctcaa atcctaaaag aaggtgacat acttgtctta
     2041 aataacacaa aggtcatccc ttccaggctt ttcgggaaaa aagacaaagg ccagcaagtg
     2101 gaggttctgc ttacggaaag gataagtcct tgtttttgga aggccattat gaaaaatcct
     2161 aaacacgact tgatgatcga atttgatgga ggtttgaagg ggaaggtatt aaaagatgga
     2221 aaagatgagt ggataattga gttcgaagag ggtgccgatg attacattga gcactctggg
     2281 aggatgcctc tcccacctta cattaagcgc atgcctgaag agaacgatag gattttctat
     2341 cagacggttt atgcggataa gaagggcgct gttgctgccc cgacagcagg gcttcatttc
     2401 acaagaaggc ttttagacga aatacgtcaa aagagcgtcg agatcctata tattacactt
     2461 catgtagggg ctggtacctt taagccggtt aagactgaga atgtagaaga gcacaaagtc
     2521 catcaagaat atagagaaat ctctaatgaa gcggctcttg ctataaacag agctaagaga
     2581 gagggcaaaa ggattgtcgc tgtgggtacc actgtagtaa gaaccctaga aggcgcactt
     2641 gataaaaatg gagatgtgca gcctgccctc ggctatacag atcttttcat ttatcctggt
     2701 tttaagttcc aaattgttga tgttttgata acgaatttcc atcttccccg ttcgacactt
     2761 cttatgttgg tctcagcatt tacaggcagg gagcttattc ttaaagccta taaagaggca
     2821 atagaaaagg gatacaggtt tctgagttat ggggatgcga tgattataat ttaggttgtt
     2881 gggttaaagg ttatatcatg caagtcgaaa ttgaaaagct cacattcggc ggggcaggaa
     2941 tggctagagt caatggtaag gtggtttttg taaagggtgg gctaccaggc gatgttttaa
     3001 aggttaaaat cacaaaggac aaaggtagtt acgctgaggc aattattgaa gaggttttaa
     3061 gaacctctcc tgaaaggact caggcgccct gtcctgtttt tggggaatgt ggagggtgcc
     3121 agttgcaaca tcttaagtat ccttctcaac tcattgctaa agaaaacatc ctaagggaaa
     3181 cccttgaaag aattggtgga ctaagaggca tcgagattga gcccataatt ccttctctag
     3241 aagaatacag gtatagaaat agggttaccc tttcaatatg gtttcaaaaa ggaaggtatc
     3301 gcctggggtt ctatgaagag gggagtcaca aacgagtccc gatagaaggt tgtccaattg
     3361 cttcgagtat tataaatgaa gcgatctccc gtcttgataa atttctttct tcgatagata
     3421 atccacgatt ccctttggtt agaattcata tagcatcgga tggaacatct gcccacattt
     3481 ctcttgttcc atccagcgag aaggatccaa agaggcttaa cggacttcgt gatcacataa
     3541 aaaaatcgct ggaggcgggg agtgtatcgg ttgcgggata taatgaggag gagtttgagt
     3601 ttaatcaatc tgggctaaag ttctattcat ctccttcggt tttcatccag tcaaatagag
     3661 aaattaatga gcgattagtt gagacgttag ttgaatggtc agatcttaaa ggacacgaga
     3721 gggctttgga cctatactgc ggggttggaa atttcttact tcatcttgca aaaagggcta
     3781 aagaggttgt aggagttgat gtcagcgcaa aggctattaa gcttgcaaaa aagggtgcag
     3841 aggcaaactc aataactaat gttaactttg attgctctcc atctgagctt tatgctgagg
     3901 aatcgctaat aaggggagat aaattcgatt tggtaatact tgacccaccg agggaggggg
     3961 cgaaagggat attgaaagca ctgggacttc tctctccgag gaaaatcatt tatgtttctt
     4021 gcgatccgcc aaccctggca agggatttaa agacgcttac tgaaattggc tataaactta
     4081 taaaaatccg tccatatgat atgttcccag agacatatca tat
//
LOCUS       DATDRL010000371         8180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_964487, whole genome shotgun sequence.
ACCESSION   DATDRL010000371 DATDRL010000000
VERSION     DATDRL010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8180
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964487"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VI935_12300"
     CDS             complement(<1..570)
                     /locus_tag="VI935_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HLE26410.1"
                     /translation="MVKIENLTLKYSGTPALIDISMEIPKYKATAFIGPSGCGKSTLI
                     RCLNRLNELIDGVTIEGSIKISGADIFDPKLDITELRKRVGMVFQKSNPFPKSIYDNV
                     AYGPRIAGIKSHSVLDEIVEKSLRSAALWDEVKERLNESAIGLSGGQQQRLCIARAIA
                     VEPEVLLMDEPCSALDPIATGKIEDLITEL"
     gene            complement(759..2390)
                     /gene="pstA"
                     /locus_tag="VI935_12305"
     CDS             complement(759..2390)
                     /gene="pstA"
                     /locus_tag="VI935_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HLE26411.1"
                     /translation="MKKFWKSGDPFIWFTGGALTFSLLMIGGLLFLIMAKGLGFFWPS
                     DIAEVQLKDGSKLLGQITGREEIPQPGAPPGTLERYRIQLQIGNRDIYGLDFRWVNEE
                     EVAKIDYPRNAVLFERREWGNMYGFIKEIREGEVVVAQGNKESWIAIKDLLPQTQGIY
                     KTIKRIEKKDIGDFNFKIEKLRLEIRKLELQGGDQASMQANIKSLEDQIEVQKKLYRN
                     KESELAGLYEKLAEDKIIVSTIDGREREMSLGAIVRAYRPNDMSLLNKALLYISKVWE
                     FISGEPRESNTEGGIFPAIFGTVMMVVIMSVVVLPFGVLAALYLREYAKQGLLLRIVR
                     ICVNNLAGVPSIVFGVFGLGFFIYAIGGTIDTLFYPESLPTPTYGTGGIIWASLTLAL
                     LTVPVVIVATEEGLAGVPSAIREGSFALGATIFETIWRVVIPSAMPAILTGLILAIAR
                     ATGEVAPLMITGVVKLAPSLPIDGDFPFIHLERKFMHLGFHIYDVGFQSPNVEAAKPM
                     VYATILLLIFIVVVLNFTAIVIRNRLRKRYATSVF"
     gene            complement(2524..4833)
                     /locus_tag="VI935_12310"
     CDS             complement(2524..4833)
                     /locus_tag="VI935_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLE26412.1"
                     /translation="MTALPKLKDQEQAAFKAFSLKRRRLADKLARVVITFGGISIILS
                     IIAILVFISLEVYSLWKGAKGELAGRFDLNKSLVISSHSVNPTSVSDFPILALGIDEY
                     REIVFVITRNGLVDFISLKDGNTVKQIPLRKIEGKKITSALGSLDNEEYTLGTDDGYI
                     LPVEIDFKVSFNDNDERIIEPEVVEGGLIKVDALPLTHVAYKALDDDTKGVAAVTSDW
                     RLVFHSQLATSSLVGEEKKEGLKYDLTPELKGSIVTAIALDQFLDNLYVGTKTGELYY
                     WDIRDNTNPRLVEVIDATENPETAITALGFLIGDRSLVVGDGSGNVSIWFQVEDKTSP
                     VGRRLKKAHTLSSHNGLVTAVSASPRDRGLLSADSRGGIILNYPTSERKLLELEGEGH
                     PIKALAFAPKADGAVAVDERGTLYDWRIENPHPEINLKALFGKVWYEGYSKPEYVWQS
                     TGGTDDFEPKFSLTPLAYGTFKGTIYALVFAIPLAILGAICVSQFMHPSLRNLIKPVI
                     EIMAALPTVVLGFLAGLWMAPLVEKILPAVFLMPAILTIMTLCSVFLWRLMPKLIRGR
                     FKEGTELLFLLPVFILGIQFCLWLNLPVENLFFGGDYKASLFNNLGLQYDQRNALIVG
                     FAMGFAVIPIIFTISEDALSNVPKHLISGSLALGATKWQTAIKVVLPTASAGIFSAIM
                     IGFGRVVGETMIVLMATGNTPIMDWSIFNGFRALSANIAVEIPEAPHGGTLYRVLFLA
                     ALLLFLVTFLVNTAAEIVRMRLRKKYAQL"
     gene            complement(4846..5874)
                     /locus_tag="VI935_12315"
     CDS             complement(4846..5874)
                     /locus_tag="VI935_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HLE26413.1"
                     /translation="MSELKTLLHLRNACLIEGVLVFILTFGSNIGFADEATKVDPKIP
                     SYTKVSGISGNINSIGSDTMNNLMTLWCEGFTKFYPNVKCQIEGKGSSTAPPALISGT
                     AQIGPMSRPIKGSEIDEFEKKFGYKPTDIRTSLDSLGVYVNKDNSLQCLTLPQVDAIF
                     SKTRKCGETEDVTTWGQVGLTGDWANRPISLYGRNSASGTYGFFKEHALCKGDYKDQV
                     KEQPGSASVVQGVTEDRFGIGYSGIGYKTSGVRTVAVSPGGGKPCKAADYQNVLDGSY
                     PLGRYLYVYINKAPNKPLDPLVGEFVKFILSKEGQQVVIKDGYDPLPESKVGEELQKI
                     KSTESARQ"
     gene            complement(6215..6694)
                     /locus_tag="VI935_12320"
     CDS             complement(6215..6694)
                     /locus_tag="VI935_12320"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HLE26414.1"
                     /translation="MKRKGVEIIDLNVKELIKDLNKALAAEGLAAYWYKLLSKVASGL
                     NSIHIAEEFEKMSEEEWKHMGVFMERIVQLDGEPIANASDWVKHSYSAYKEPPKDPYD
                     LEKMIRDSLEAEREAIVFYNNLYQKTQHADPVTADLVVKILADEVEDEDKLLRLLSK"
     gene            complement(6921..7874)
                     /gene="cyoE"
                     /locus_tag="VI935_12325"
     CDS             complement(6921..7874)
                     /gene="cyoE"
                     /locus_tag="VI935_12325"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HLE26415.1"
                     /translation="MKQEAKVKSAEIAPKRILVSTLLLLKPRIILSVAFTGFAGMVLA
                     NRGFPSLDIIIFGIVSLLLSAAGAAILNNILDKQIDVLMKRLGRRVEALNVVGEKTAL
                     FIALLSILISLFISFYFLNFVNSILIIAALLSYTFLYTLYLKRSSPYGTIPGGLPGAL
                     PVLIGYSAVKPNLGIDGIILFLIMILWQPPHFWALAQTYKNDYKNAGVPVMPVALGTK
                     YTNIFMLLYSLSLFPLSLSLWFLGYCSSLYAIVAIIVGIYFEYVMIRSVLTNSNYGKA
                     FGVSILYILVIMASLIIDISLGSADMVLQSTEISRPLKTIP"
     gene            complement(7947..>8180)
                     /locus_tag="VI935_12330"
     CDS             complement(7947..>8180)
                     /locus_tag="VI935_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26416.1"
                     /translation="FAAVLLYGFSFLNAELNYKRSKILILILLTILQVIVGISVIHSK
                     LYFLATAFHLAIALFILSIVLYIWFQVMVEKRV"
BASE COUNT         2486 a         1756 c         1464 g         2474 t
ORIGIN      
        1 aagctctgtt attaggtcct cgatttttcc tgtagcaata gggtcaagag cagaacatgg
       61 ctcatccata agaagcacct caggctcaac agcaattgct cttgcaatac aaagcctctg
      121 ctgttgtcct cctgaaagcc cgattgcgct ttcatttagt ctttccttta cctcatccca
      181 aagcgcagca cttcttagac tcttttccac tatctcatct aaaacggaat gacttttaat
      241 tccggcaatt ctaggaccgt atgcaacgtt atcataaatt gattttggaa aaggattcga
      301 tttttgaaaa accataccga ctctttttct cagttctgta atatctagct ttggatcaaa
      361 tatatccgcg ccacttattt taattgagcc ttctattgtt acaccatcaa tgagttcgtt
      421 tagacgattc aagcaacgaa taagtgtgga ctttccacat cctgagggcc caataaaagc
      481 ggtagcctta tactttggga tttccatgct tatatcaatt aaagcgggtg tgccactata
      541 ctttagggtg agattttcaa tcttgactat cgggtttgga atatctactt gttgacccac
      601 tcttttctct tcgaattcat cggtctttat aatagtatca tactcttcat tttcctctaa
      661 cttcttattt tctaagagaa attccacaat gccctccttt tttcggggtt attgtctcgg
      721 ggttcgattc acgattcacg aatcacgata cacgcatttt aaaacactga tgtagcgtat
      781 cttttcctaa gacggttcct tataactatt gcagtaaaat ttaaaaccac aactataaaa
      841 atcaggagta atattgttgc ataaaccata ggcttagctg cttcaacatt tggagattga
      901 aacccaacat catagatgtg gaagccaagg tgcatgaatt ttctttcaag gtgtataaaa
      961 ggaaagtcgc cgtcaattgg gagtgaaggg gcgagtttta caacccctgt aatcataagg
     1021 ggtgcaacct ctccagttgc tcttgctata gctaagatta gacccgtaag aatcgctggc
     1081 attgcgctcg gaatgactac tctccaaatg gtttcaaata tcgttgcgcc aagggcgaat
     1141 gaaccttctc ttattgcact tggaacccct gcaagcccct cctctgtagc aacaattact
     1201 accggcacag taagaagagc aagcgtaagc gatgcccaga tgattcctcc tgtgccgtaa
     1261 gtcggtgttg gtagggactc aggataaaaa agggtgtcaa tagttccccc aattgcgtat
     1321 ataaaaaaac cgagtccaaa aacgccgaat actattgagg ggacccctgc caggttatta
     1381 acgcagattc ttacgattct taagagcaac ccttgctttg catactctct taaatataag
     1441 gcagcaagaa ccccaaaggg taaaacaaca acgctcatta ttactaccat catgactgta
     1501 ccaaaaatgg cagggaagat accaccttcc gtgtttgatt ctctgggctc accagaaata
     1561 aactcccaaa cctttgaaat ataaagaagc gctttattaa gcaagctcat atcatttggc
     1621 ctataagctc gaacaattgc gcctaaagac atttcccttt ctcttccatc tatagttgaa
     1681 acgattatct tatcttcagc gagcttctcg tatagaccgg caagttcgga ttctttgttt
     1741 cgatatagct ttttttgaac ttcaatctga tcttcaaggc ttttgatatt agcttgcata
     1801 gaagcttgat ccccgccttg aagctctagt ttccttattt ctagcctaag tttctcgatt
     1861 ttaaagttaa aatcccctat atcctttttt tcaatccttt ttatcgtttt gtaaatccct
     1921 tgtgtttgag gaagaaggtc tttaatagca atccaggact ctttattccc ctgagcaact
     1981 acaacttctc cttcccgaat ctccttaata aacccataca tattccccca ttcacgcctt
     2041 tcaaaaagaa ctgcgttacg tggataatct attttagcaa cctcttcctc gttaacccat
     2101 ctaaaatcca aaccatatat atcccggttt ccaatctgga gctgaattcg atagcgttca
     2161 agggttccag gaggcgcgcc aggttgtggg atttcttcac gacccgttat ttgtccaagg
     2221 agtttacttc catccttaag ttgtacttcg gcgatatcag agggccagaa gaaacccaac
     2281 cccttggcca taattagaaa aaggagccct cctatcatga gcaggctaaa tgtgagcgcc
     2341 ccgccagtaa accatataaa agggtcaccg cttttccaga actttttcat cttttcaact
     2401 aaccctaatt aaattgctac taatgtacac agatttacac gaataaaata atttttaagt
     2461 ctttcatatt taattttttt ctttttattt gtgttcattc gtgttaatcc gtggccaatt
     2521 tatttataac tgagcgtatt ttttcctgag tctcatcctt actatctccg ccgctgtatt
     2581 tactaagaac gtcactagga aaagaaggag tgcggcaagg aataaaaccc tatagagggt
     2641 tccaccatgt ggtgcttcag gaatctcaac tgcaatattg gctgacagtg ccctaaatcc
     2701 attaaatata ctccagtcca taattggcgt gttccctgtt gccataagaa caatcatcgt
     2761 ttcaccaaca acacgaccaa aaccgatcat tatagcagaa aagattcctg cacttgctgt
     2821 tggaagcact accttgattg cggtttgcca cttagtcgcg ccgagcgcta aggaacctga
     2881 aattaggtgc ttcgggacgt tagatagtgc atcctctgaa atagtaaaaa taataggaat
     2941 aacggcaaac cccatagcaa atcctacaat caaggcgttt ctttgatcat attgaagccc
     3001 cagattgtta aaaagtgatg ccttgtagtc tcctccgaaa aacaggtttt caactggtag
     3061 atttaaccag agacaaaatt gaatcccaag gataaatacg ggtaaaagaa aaagtaattc
     3121 agttccctct ttaaatcttc cccgaattaa cttaggcata agtctccaga gaaatacaga
     3181 gcataaagtc ataatggtaa gaatcgccgg catcaaaaaa acagctggta gtatcttttc
     3241 aacaagtggt gccatccata ggcctgcaag aaaacccaaa acaactgttg gtagtgcagc
     3301 cattatctca ataacaggtt taattaagtt tcttaaagaa ggatgcataa attgagagac
     3361 acaaatggcg ccgagtatag ctaatgggat ggcaaaaacg agtgcgtaga ttgtcccctt
     3421 aaaggttcca taggcgagtg gggtgaggct aaatttaggc tcaaaatcat cagttcctcc
     3481 ggttgattgc cagacgtatt caggtttgct ataaccttca taccaaacct ttccaaaaag
     3541 ggcctttaaa tttatctcag gatgcgggtt ttcgattctc caatcatata aagtacccct
     3601 ttcatcaacc gccactgcac cgtctgcctt aggagcaaag gcaagtgcct ttataggatg
     3661 cccttccccc tccagttcaa gaagcttccg ctccgaggtt gggtagttaa gaataattcc
     3721 ccctcttgaa tctgcgctta aaagacctct gtctcttggg gaagcggaga cagccgttac
     3781 taatccgtta tgtgaagaaa gtgtatgagc ctttttaagc cttctgccta caggtgatgt
     3841 tttatcttca acctgaaacc agatagaaac attgccagaa ccatccccaa ctacaagaga
     3901 acggtctcca attaggaatc caagggcagt tatcgctgtc tctggatttt ccgtagcatc
     3961 aataacctcc acaagccttg gattcgtatt atcccttatg tcccagtaat aaagctcacc
     4021 cgtttttgta cctacataca gattatcgag aaattggtca agggctatag ccgtaactat
     4081 gctaccctta agctctggag tgagatcata ttttaatccc tctttttttt cttcaccaac
     4141 taaagaacta gtggccagtt gagaatgaaa aacgagtctc caatccgagg tgactgctgc
     4201 aacccctttt gtgtcgtcgt ctaaggcttt ataagcaaca tgtgtaagag gaagagcatc
     4261 aaccttaatc agtcctccct ctactacctc aggctctatt attctttcgt cattatcatt
     4321 aaagctcact ttaaaatcaa tctcaacggg taaaatataa ccatcatctg ttcctagggt
     4381 atattcctcg ttatctagtg agcctagagc cgatgttatc ttctttcctt ctatttttct
     4441 tagagggatt tgcttaacgg tgtttccatc cttaagagat ataaaatcaa ctaagccatt
     4501 cctggttatc acaaatacta tttccctgta ctcatctata ccaagcgcaa gaatggggaa
     4561 atcagacact gaggtaggat taacggagtg tgatgaaatt acgagagatt tattaaggtc
     4621 gaatctacca gcgagctctc cctttgcccc tttccagagt gagtaaacct ctagagaaat
     4681 aaagacaagt atagcaataa tgctgaggat aatagaaata ccaccgaaag tgattactac
     4741 gcgtgctaat ttatctgcaa gccgccggcg tttaagtgaa aaggctttaa aggcggcttg
     4801 ttcttgatcc tttagctttg gaagagctgt cataatagaa tagacttatt gtcttgcaga
     4861 ttcggttgat tttatctttt gaagctcttc accaacttta ctctctggaa gtgggtcata
     4921 accatctttg attacaacct gctgaccttc tttgctcaga ataaacttaa caaattcacc
     4981 aacaagtgga tcaagaggtt tatttggagc tttgttgata taaacgtaca ggtatcttcc
     5041 cagtggatat gagccatcca gaacgttttg gtaatctgct gccttgcaag gcttaccacc
     5101 ccctggagaa accgcaactg tacgaacccc tgaggtttta tagcctatgc cactgtaacc
     5161 tattccgaaa cggtcttctg tcacaccctg cacaactgaa gcagagcctg gctgttcttt
     5221 gacttggtct ttatagtctc ctttacaaag ggcatgctct ttgaagaacc catatgtccc
     5281 tgatgcagaa ttcctaccgt ataaacttat tggacggtta gcccaatcac ctgtaagacc
     5341 aacctggccc catgttgtta catcttcagt ttcaccgcat tttcttgttt tcgagaatat
     5401 tgcgtcaact tgtggcaagg ttaggcattg tagagaatta tctttattaa catagactcc
     5461 aagagagtca agagaggtac gaatatccgt cggcttatag ccaaacttct tttcaaattc
     5521 atctatttct gaccctttta tagggcggga cataggacct atctgagcag ttcctgaaat
     5581 caacgctggg ggagcagtac tcgaaccctt tccctcaatc tggcatttta catttggata
     5641 aaacttagta aatccttcac accataatgt cataaggtta ttcatcgtgt ctgaaccaat
     5701 actgtttatg tttccagata taccgctaac cttcgtgtaa gatggtatct taggatcaac
     5761 ctttgtagcc tcatcggcaa aaccaatatt acttccaaag gtgagaataa aaaccaaaac
     5821 gccttcgatt aagcaagcgt ttcttagatg taacaatgtt tttaattcac tcataactat
     5881 gattctcctt actaactaat tttcttttac tatctataaa ttaaacttta aaatgttaag
     5941 aaattgttaa gagtttatta acttcacgtt aatagagaat ctaataccat ttccatctat
     6001 taagtgttca aaaaaatttt ctgaatttca tcgtaggagc gtaatccctt cgctaagcct
     6061 ttgctgagtt tatcgaaggg ctcagaacga acgggtctta agccttgtac ctaatagcag
     6121 cattataaac tgtttcttgt tggtaatagt attactagga ataaccaagt tttcaggata
     6181 aaagaatgac tcgataaatt cactaatcct atatctactt actcaagagt ctaagaagct
     6241 tatcttcatc ctcaacttca tcagcaagaa tttttactac caagtccgcc gtaactggat
     6301 ctgcatgctg tgtcttttga taaagattat tatagaatac aatcgcttcc ctttcagcct
     6361 ctaagctgtc tcgaatcatc ttctctagat cgtacgggtc ctttggaggc tctttatagg
     6421 cagaataaga atgttttacc caatccgaag catttgcaat tggctcacca tcaagttgaa
     6481 caattctttc cataaaaact cccatatgtt tccattcttc ctcggacatc ttttcaaatt
     6541 cttcagctat atgaattgag tttagtcctg aagctacctt tgaaagcagc ttataccagt
     6601 atgcggcaag tccttctgcc gctaaggctt tatttagatc cttaattaac tctttgacat
     6661 ttaagtctat aatttcaact ccctttctct tcattttcta attcctcctt ataaacggta
     6721 ataattacca tataataatt ggtgtgattt gtcaatacat tattgaacgt tatgttcact
     6781 gctacagata tttaatgtat taaatacgat tatacatccc cattccttaa cggataatgt
     6841 aagttaaatt taaaataatt tgtaattgaa tcgtttagaa ttaaatcagg aactgagtta
     6901 gcgatcccgt ggattgtagt ttaaggaatc gtttttagag gtcttgagat ctcagtagat
     6961 tgcaaaacca tgtctgctga gcctaaagaa atatcaatta taagagaggc cataattacc
     7021 aatatatata aaattgacac accaaaggct tttccatagt tagagttggt aagaacactt
     7081 ctaatcatta catattcaaa ataaatacca actataatag cgactattgc atataaacta
     7141 gagcaatatc ctaagaacca taaagataaa gaaagaggaa aaagggaaag cgaatagagg
     7201 agcataaata tatttgtata ttttgtgcca agtgcaaccg gcatcacagg cactcccgca
     7261 tttttataat cattcttata tgtctgagca agcgcccaaa aatgaggggg ttgccagaga
     7321 atcataataa gaaataggat tatcccatca attcctagat taggttttac cgcagaataa
     7381 ccaataagaa ctggaagggc accagggagt ccaccgggaa tcgttccata tggagaactg
     7441 cgcttcagat aaagcgtata aagaaatgta tagcttagaa gagcagcgat aatgagaatg
     7501 ctatttacaa aattaagaaa ataaaaagag ataaataggg aaattaatat ggataaaagt
     7561 gcaataaaaa gggctgtttt ttctccgact acgttaagag cttcaacacg cctgcctaat
     7621 cttttcatta aaacatctat ttgcttgtca agtatattgt ttaaaatcgc agccccagcc
     7681 gcgctcaaaa gcaaagacac aatgccgaaa ataatgatat ctaaagaggg aaacccccta
     7741 tttgcaagta ccattccagc aaatccagta aaagctacac ttagtataat tctaggtttt
     7801 aaaagaagta gggttgaaac gagaatcctc ttaggagcta tttccgctga ttttacctta
     7861 gcttcttgct taattttagg ccctccttag tggattgtga ctcgtggttc gtgtctagtg
     7921 attcgaaaca caattcacga gataaattat acacgtttct ccaccataac ttgaaaccat
     7981 atatagagaa ctatggagag tatgaaaagg gcaatcgcta ggtgaaaagc agttgctaag
     8041 aaataaagtt tagagtggat cacactaatc cctactataa cttggagaat agtgagaaga
     8101 attaaaatta aaatttttga gcgtttataa ttaagctcag cgtttaaaaa tgaaaagccg
     8161 taaagtaaca cagcagcaaa
//
LOCUS       DATDRL010000372         8403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1758452, whole genome shotgun sequence.
ACCESSION   DATDRL010000372 DATDRL010000000
VERSION     DATDRL010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758452"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2122)
                     /locus_tag="VI935_12335"
     CDS             complement(<1..2122)
                     /locus_tag="VI935_12335"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
                     /protein_id="HLE26417.1"
                     /translation="MRCPSCGFENPLEIKFCGECGASLKNICPNCGFKNPPGFKFCGE
                     CATPLIDQAQVSKSTDIDRQPYKQEDKGAQIIPIVSERKAPEAERRQLTVMFCDMVGS
                     STLSELLDPEELRDVMRNYQDVCSDVIRRHDGHIAKYLGDGLLVYFGYPLAHEDDAQR
                     AVRAGLEIVGAVRELPLHTRLQQPLQIRIGIHTGLVVVGEMGAGDVREKMAVVGETPN
                     IAARLQELAETNSVVVSSTTHRLIQGFFDGRELGPHTLKGFSQPMEVYQVLYESGVQS
                     RFEVAVTKGLTPLVGREQEVELLLERWERVKEGEGQVVFLIGEAGIGKSRLVQVLKEH
                     VAAESHTRIESLCSPYYQNSSLYPVIDHLQRLLEFRREDSPQEKLNKLERVLERYGFS
                     LQEMVPLFASLLSLPIPESYPSLTLTPQRQKQKTLEALLTWLLKEAEKHPVLRIVEDL
                     HWMDPSTLEYLSLLVEQVPKTRIFALFTFRPDFNPPWGARSHVTQITLNHLTWKQVEV
                     MVEREAGGRALPAEVVQQVVAKTDGVPLFVEELTKMVLESELLRERGGHYELMGPLPP
                     LAIPSTLQDSLMARLDRLATVKEVAQLGATLGREFTYELLHAVLPLDEETLRRELAKL
                     VEAELLYQRGLPPQARYFFKHALIQEAAYQSLLKSKRRQYHQKIAQVLEERFAEIRET
                     QPELLAYHYTEAGLSGQAIVYWHRA"
     gene            complement(2365..3108)
                     /locus_tag="VI935_12340"
     CDS             complement(2365..3108)
                     /locus_tag="VI935_12340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLE26418.1"
                     /translation="MSYEEIENKVALITGGTEGIGFGIAKSFVEAGAKVAITGLSKLQ
                     TAKEEFGDKVLTINADITKVKLCKVTLDKVLGEYGKLDILVNNAGTNIYKPVSQTTIQ
                     EFDIIFNTNVRGLFALTQAAIPELKKTHGNIINIGSVFGFRGMPIYSVYSASKAAVIM
                     LTQLWAKELAPDVRVNTISPGGIDTAIFKKMYGEEKHQQVLEFVSGRHLMKRMGQPEE
                     IARMALYLATENWVTGSNFVVDGGLFHLI"
     gene            complement(3330..4709)
                     /locus_tag="VI935_12345"
     CDS             complement(3330..4709)
                     /locus_tag="VI935_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26419.1"
                     /translation="MASYEEIRQQHIAVLTGMIPEHLERLNWSRRQIDAERQSRLREL
                     VGVAKERSPWHAKRLTHINADTVTEVHLEAIPPMTRDDLMSNWNDIVTDRRLSLELVE
                     GHLDSHDNHDYLMDEYTVCASGGSSGRRGVFVYGLKGCATAYMAGLRHRIRKQVELAG
                     ESSGTNRLAYLGSHHPAHMAYVFHQIFGFPGIDSLLLLGVQDPIEEVVCRLNEFKPTH
                     LIGYPTALQLLAREQKEGRLCISPSHVSASSEPLFNEIREALTEVWGVVVLNIWAMTE
                     AGACGHSIGQAPGLMMNDDLSIIEAVDSEGRYIPRGMCSNKIFITNLFNHVLPLIRME
                     VTDEITLLVEGDPGGSGHQMIAEVEGRKDDLFIYNDGKHIVHPHVFRSVLGRERSIIE
                     YQVRQTPNGAQILVVTAAQVGIQELERKIADALTRIGIEKPGIVISHVDKIDRSATGK
                     LKRFVSLSI"
     gene            complement(4874..5899)
                     /locus_tag="VI935_12350"
     CDS             complement(4874..5899)
                     /locus_tag="VI935_12350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLE26420.1"
                     /translation="MKFGIGPFSMRKHPENPGSHAEIYRQTLEQVKLAEEVNFDSAWI
                     AEHHFLEDGFCPSSIVAASAMAPVTKRIKIGTCALLLPLHNAVRVAEDAAVVDNISNG
                     RFILGVGMGYNREEFNGFGIPMNQRPSRMEEGINVIVKSWTENNFSYEGKRYRFSNLN
                     VTPKPVQKPHIPIYIGAVEEPAIRRAGRLGYPLLINPIRTMQMIRDTINIYNYAAGKE
                     GKNPNSTPHILIREVCIAENKKKEKEEGDRFLKSRNKFYSGLGVKFIVRGKQITGLDD
                     PLFDYFAEDRFIIGDPDDCIREVKRYRDELGINYITCLMALPTATHETISRCIELFGR
                     EVIPSIS"
     gene            complement(5994..>8403)
                     /locus_tag="VI935_12355"
     CDS             complement(5994..>8403)
                     /locus_tag="VI935_12355"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLE26421.1"
                     /translation="GLGQVVTIIGDAGIGKSRLLYEFRKLLEDEKILYLEGRCISYGK
                     SISYLPIIDIIKIRFRIDKLDTERTIRKKIEKGISDIDPNLERSIPFLYDILSIETDA
                     PLLKSLDLRDKRKRTLEALKNIIIADSNLRPLVVAVENLHWLDGASEEFLPFLIDNIP
                     SSKIMLILTSRPGYRAKWSEKSYHIQIAPRRLSDKEVEEMIKSILGSEKATGELIKFV
                     TNKADGIPFYVEEIIKSFTEAGIIVKGEDGYSVREGIAGVDVPDTIQDIIMARIDRLE
                     ENLKKTLQYASIVGREFSYKLLKEIMEIGEELQDYLTQLKGLELVYEKSLFPDLEYRF
                     KHSLTQEVAYNSLLIKKRKELHGKIGEIIEDLSGDKLEENYELLAYHYGRSTSDEKAV
                     DYLTLAGDKSAKIYSTEDAIGNYEEALKRLDNMPDARANKEKRVDIFIKQARVMRLMG
                     KFKEHIKTLEKNLPIVEELGDKDRLAEYYFKMGFYYSVMGSTEDAIQYCTKAIELAEL
                     TKNERIIGLASLRLGYSYWYKSEPRKGFPFAKKAINIMEKLGDIYWVGRAFQTLGVLH
                     WMMGDWDKSIEYMETFLRKSKEVSDVNLLSLAYYSIAMPLVEKGEWDVAIDYCKKCLD
                     ISPTPLFAVYGLGFLGYGYCKKGEWERGIKDLEKAIQQSKDFGLGHQEAQFSAYLAEA
                     YLTINERDKALERTKSALEISKESGFRHLEGMAYRVLGEIYSEAKSNEPKKYIEDSIR
                     ILNEVGAKNELAKSYLSLGRLYKEKGEKAKAKKYLTQALHIFEKLGTLHEPEKARKLI
                     HELK"
BASE COUNT         2103 a         2078 c         1609 g         2613 t
ORIGIN      
        1 ctgccctatg ccaatagaca atggcctgcc cgctgaggcc tgcctccgtg tagtgatagg
       61 caagaagctc aggctgagtc tctctgatct ctgcaaaccg ctcctccaat acctgtgcaa
      121 tcttttgatg gtactgccgc cgtttgctct ttagcaatga ctgataggct gcttcctgaa
      181 tcagggcatg tttgaagaag tacctagcct gaggtggaag tcccctctgg tagagaagtt
      241 cagcctccac taactttgca agctctctcc gtagagtctc ctcgtccaga ggcaaaacag
      301 catggagtaa ctcataggta aactcccgcc ccaaggtggc tcctagttgt gccacctcct
      361 tgactgtggc tagccgatct aacctggcca tgagtgagtc ttgtagcgtg gaaggaatcg
      421 ctagtggggg taggggaccc ataagctcat aatggcctcc tcgctccctg agcaattccg
      481 actctagcac catctttgtc aactcttcta caaatagagg tactccatcg gtcttggcca
      541 ctacctgctg aactacctca gcaggaagag ccctgcctcc tgcctccctt tcgaccatca
      601 cctctacctg tttccaggtt aagtggttta gcgtgatctg ggtcacgtgt gagcgggcgc
      661 cccatggcgg gttaaaatcc gggcgaaatg tgaacaaagc aaagatacga gtctttggta
      721 cctgttctac aaggagactt agatactcaa gagttgaggg atccatccag tgtaggtctt
      781 ccactatacg cagcaccgga tgtttctccg cttccttcag cagccaagtc agcaaggctt
      841 ctagggtctt ctgtttctgc ctctgaggtg tgagggtgag agatgggtag ctctcaggaa
      901 tcggtagaga aagcaaagag gcaaaaagtg gcaccatctc ctgaagggaa aaaccatacc
      961 gctccagaac cctctcaagc ttattgagct tctcctgcgg ggaatcttcc ctcctaaact
     1021 caagcagccg ctgcaaatga tcgattacgg ggtatagaga actattctga taatagggcg
     1081 aacagaggct ctctatcctc gtatgtgact ctgccgccac gtgttctttt aacacctgta
     1141 caaggcgtga tttgccaata cctgcctcac cgattagaaa caccacctgc ccctcacctt
     1201 ccttcacccg ctcccagcgc tccaaaagaa gctctacctc ctgttccctt cccaccagtg
     1261 gggtcagacc tttagtgaca gctacctcga agcggctctg gacaccactc tcatagagca
     1321 cctggtacac ctccatcggc tgggagaagc ctttgagggt gtgaggacct aattcccgac
     1381 cgtcaaaaaa tccctgaata agcctatggg ttgtggagct aaccacgact gagttagtct
     1441 ctgctagttc ttgcagcctg gcagcaatgt ttggagtttc tcctactaca gccatttttt
     1501 cacgaacgtc tcctgccccc atttctccca caacaacaag accagtatga atgccaatgc
     1561 gtatttgtag gggttgttgt aaccgcgtgt gtaggggcag ttcgcgaact gcccctacaa
     1621 tttccaatcc cgctctgacc gccctttggg cgtcgtcttc atgtgctaag ggataaccaa
     1681 agtaaacaag cagaccatct cctagatatt ttgcaatatg tccatcatgg cgccttataa
     1741 catccgagca gacatcttga taattgcgca tcacatcccg tagttcttct ggatctagca
     1801 gttccgaaag ggtggaagag cctaccatat cgcaaaacat tacggtaagt tggcgacgtt
     1861 cggcttcggg cgctttacgt tcagacacta tcggtatgat ctgagcgccc ttgtcttctt
     1921 gtttatacgg ctgtctatct atatcggtgg atttggagac ttgagcctga tctatgaggg
     1981 gagtggcaca ttctccacag aacttgaacc ctggtggatt cttaaaaccg cagttcgggc
     2041 agatgttctt aagtgaagca ccacactcac cacagaactt aatctctaac ggattttcaa
     2101 agccacagct tggacagcgc attcacccaa cccccaaagc ctggtttaaa catattctat
     2161 catatatatg ctatttacac ttacttccta cttcttcaag gttccacttc cgtaatcgtt
     2221 ttaactttta gcttcggcaa ttgaatggag ctatagtttc ggtctcatct tctccaaagt
     2281 cccctacgtt gtcattctct cccttatatt cctaaaaacg tgaaggccag tgtcagacat
     2341 ggttacttgt tttggtggag aggttcagat taggtgaaat agcccgccat caacaacgaa
     2401 attacttcct gttacccagt tttcagtagc gaggtaaagt gccatccgtg caatttcttc
     2461 aggttgtccc attcttttca taagatgacg accactcacg aattcaagta cctgttgatg
     2521 tttctcttca ccatacatct ttttaaagat ggcagtgtct attccaccgg gacttattgt
     2581 attcaccctc acatcagggg cgagttcttt agcccacaac tgtgttagca taatcactgc
     2641 ggctttggaa gcagaataaa cgctatagat aggcatcccc ctaaaaccaa agactgagcc
     2701 gatgttgatt atattcccat gggtcttctt aagctctgga atcgcagcct gggttagtgc
     2761 aaagagacct ctaacattgg tgttaaaaat gatgtcgaat tcttgaattg ttgtttgaga
     2821 tactggtttg tagatattgg ttcctgcgtt gtttacaaga atatccaatt tcccgtactc
     2881 cccgagaact ttatcgagag ttactttaca aagctttact ttggtgatgt cggcatttat
     2941 ggtcagaact ttatccccga actcttcttt agccgtctga agtttactta gtcccgtaat
     3001 tgctacttta gcgcctgcct caacaaaaga ttttgcaata ccaaaaccaa ttccttctgt
     3061 ccctccggta ataagggcaa ctttgttttc tatctcctcg taactcatga tgcacccccc
     3121 tcctttattt caaacaaaaa ttaaaaaaac tttataagcg gttattgctt ggtttaatat
     3181 tggatattac cctagatccc ttaatatctt ccgtgccttt tcgggctcct atttagttgg
     3241 ctatagggga agcaatctac taattcctca aaaggtggaa tctataatct cttcttattc
     3301 ccatttcgca tgctaccacc tgtttacgct tatattgaaa gcgatacgaa ccgtttaagt
     3361 tttccggttg ccgagcgatc tattttatct acgtgactaa tgacaatccc aggcttctca
     3421 atcccaatcc tagtcaaggc atcagcaatc ttgcgttcta gttcctgaat cccaacctgt
     3481 gcggcagtaa caaccagtat ttgtgcccca tttggggtct gccgtacctg atactcgatt
     3541 atactccgtt cacgaccgag aaccgacctg aatacgtggg gatgaacgat gtgcttccca
     3601 tcattataaa tgaagagatc atcctttcta ccctcgacct ccgcaatcat ctggtgcccg
     3661 gaaccacctg gatcgccttc cactagaaga gttatctcgt cggttacctc catacggata
     3721 aggggaagta catgattaaa aagatttgta atgaaaatct tattagaaca cattcctcta
     3781 gggatgtacc ttccctcact atccactgcc tcaataatcg agaggtcgtc attcatcatc
     3841 aaaccaggcg cctgtccgat actatgacca catgcacccg cctcagtcat tgcccatata
     3901 tttagcacca ctactcccca tacctcggtg agagcttcac gaatctcatt aaatagtggc
     3961 tcacttgatg cagatacgtg gctaggggag atacaaagac gaccttcttt ctgctcacgt
     4021 gcaaggagtt gaagtgcagt gggatagcct attaggtgag taggcttgaa ttcgtttagt
     4081 cggcacacca cctcttctat cgggtcttgt actccgagca gcaagaggct atcgattccg
     4141 gggaagccga atatctggtg aaagacataa gccatatgtg cagggtggtg ggagccgaga
     4201 taagctagcc gattggttcc gctactttcc cccgctaatt ccacctgctt ccggatacgg
     4261 tgtcgcaatc cagccatgta agcggttgcg caacccttta gaccataaac gaagactcca
     4321 cggcgcccac tcgaaccacc tgatgcacaa accgtatact cgtccatgag gtagtcatgg
     4381 ttgtcgtgtg aatcgagatg accttcgaca agttccagac ttagacggcg gtcggtgacg
     4441 atatcgttcc agtttgacat gagatcatct ctggtcatgg gggggattgc ttcgaggtgc
     4501 acttcggtga ctgtgtcagc gttgatgtgg gtaagcctct tagcgtgcca tggcgatcgt
     4561 tcctttgcaa ccccgaccag ttctcgcaaa cgggactgac gttccgcatc gatctgccgg
     4621 cgagaccaat ttagacgttc caaatgttcg ggaatcatcc cggtaagtac tgcaatgtgt
     4681 tgctgtctga tctcttcgta gctagccatc accttttact tataaatatg ggactgtatt
     4741 taaatacctt attctcaata atctttggat tttttcgaag aaaaataatc cattcctaaa
     4801 actgaatccg aactttttta gagcctactt tatgcatcaa cctttccttt tttggtatgg
     4861 aataagataa ctattaacta atgctaggaa taacttccct tccaaaaagc tctatgcacc
     4921 tcgatattgt ctcgtgtgtt gctgtgggga gtgccattaa acaggttatg taattaattc
     4981 caagttcgtc tctatatctc tttacttccc ttatacaatc atctggatcg ccgattataa
     5041 aacggtcttc agcaaagtaa tcaaataaag ggtcatctaa acctgtaatt tgttttcctc
     5101 ggacaatgaa tttaactcct agaccagaat aaaacttatt cctgcttttt aagaatctat
     5161 caccttcctc cttctccttc ttcttgtttt cggcaataca aacttccctt ataagaatat
     5221 gcggagtact attgggattt tttccttcct ttcctgcagc ataattatag atatttattg
     5281 tgtctcttat catctgcatt gttctaatgg gattaatgag aagcgggtaa cccaatcttc
     5341 ctgcgcgcct gatcgcgggt tcctcaaccg cacctatata aattggaata tgcggtttct
     5401 ggactggttt tggtgtaacg ttaaggttac taaacctgta acgctttccc tcatatgaga
     5461 agttgttttc ggtccatgat ttaacaatta cattaattcc ttcttccatt cgagagggcc
     5521 tttgattcat gggaattcca aatccattga attcctctct attgtaaccc atgccaacac
     5581 ctagaataaa tcttccattc gatatgttat ccacaactgc ggcatcttct gctactctta
     5641 cagcattgtg aagcggaagt aaaagggcgc acgttccgat ttttatccgc tttgtcactg
     5701 gagccattgc agaggccgca acaatcgagg atggacagaa accatcctcg aggaagtggt
     5761 gttctgcgat ccatgctgaa tcaaaattca cctcttctgc gagttttact tgttcaagtg
     5821 tttgtctgta aatctcagca tgagaaccgg gattttcagg atgtttccgc atgctaaatg
     5881 gtccaattcc aaatttcatc tatttacctc tctttactag aggggtttag ccattttttg
     5941 gtaaaaaact tttctaattt tctttcccga aactaaaata gagacatctg tctctatttt
     6001 aattcatgta ttagcttcct tgccttttcg ggctcgtgta gtgtacccag cttttcaaag
     6061 atgtggagag cctgggtgag atatttcttc gccttagctt tttcgccttt ttctttgtat
     6121 aaccttccta agctaaggta actcttagca agctcgtttt ttgcccctac ttcgttaagg
     6181 attctaatgc tatcttcaat gtacttcttg ggctcattgg atttagcttc actgtatatt
     6241 tcccctaaga ctctataagc cattccctcc aaatgcctaa agccactttc tttactaatc
     6301 tctaaagcac tttttgttct ttccagagct ttatcccttt cattaatggt taaataagcc
     6361 tctgctagat aagcactaaa ttgagcttct tggtgtccca aaccaaaatc tttagattgt
     6421 tggatagctt tctccaaatc cttgattcct ctctcccatt cacctttttt gcaatacccg
     6481 tacccgagga aacccaaccc gtatactgca aataagggag taggagatat atccaaacat
     6541 tttttacaat aatcaatcgc tacatcccat tccccttttt ccacaagggg catggctata
     6601 gaataatatg ctagagatag taagttaaca tcagagactt ctttgctttt ccttagaaat
     6661 gtttccatat actcaatact tttgtcccag tcacccatca tccaatgaag tacccctaaa
     6721 gtctgaaacg ctctgccaac ccagtagatg tctcctagct tttccattat atttatagct
     6781 ttcttagcaa agggaaatcc cttacggggt tcacttttat accaatagga atatcctagc
     6841 ctgagtgatg ccaatcctat gattctttca ttttttgtta actcagccag ctcaatcgct
     6901 ttcgtacagt actgaattgc atcttcagta cttcccatca ctgagtaata aaatcccatt
     6961 ttaaagtagt attctgctag cctgtctttg tctccaagct cttctactat tggtaggttc
     7021 ttttctaagg tttttatgtg ttccttaaat ttacccataa gacgcataac ccttgcctgt
     7081 tttatgaata tatctaccct cttttcttta tttgccctgg catcaggcat gttatccagt
     7141 ctcttaaggg cttcttcata gttgcctatc gcatcctctg tagagtaaat ctttgcggac
     7201 ttatccccag ctaaggtcaa ataatcaaca gccttttcat cactggtgct tcttccatag
     7261 tggtatgcca atagctcata attctcctcc agtttatctc cagataagtc ctctattatc
     7321 tctcctatct tcccgtgtaa ttcttttctt tttttaataa ggaggctgtt ataagctacc
     7381 tcctgtgtaa ggctgtgttt gaatcggtat tctaagtctg gaaagaggct cttctcgtat
     7441 actagctcta agccttttag ttgagtaaga taatcctgaa gctcttctcc tatctccatt
     7501 atctctttta gcaatttata gctgaattcc ctaccaacga tagaggcgta ttgaagggtc
     7561 tttttaagat tctcctccaa cctatcaatc cttgccatta taatgtcctg tatagtatca
     7621 ggtacatcca ccccggctat tccctcccta acactatagc catcttctcc tttaactatt
     7681 atccctgcct cagtgaaaga tttaataatc tcttctacgt aaaagggaat tccatcggct
     7741 ttgtttgtaa caaatttgat tagctcccca gttgcttttt cactacctaa gattgatttt
     7801 atcatctcct ctacttcctt gtcagacaga cgtcttggag caatctgaat gtgataggat
     7861 ttctcactcc atttagccct ataaccgggg cgacttgtaa gaattagcat tatcttactg
     7921 ctgggtatgt tatctataag gaagggtaag aactcctcag acgccccgtc taaccagtga
     7981 agattctcaa ccgctacaac aagcggtctc aagttgctat cggctataat tatattcttt
     8041 aaggcctcaa gtgtcctttt ccttttatcc cttaagtcta agctcttaag gagaggggca
     8101 tctgtctcaa ttgaaagtat gtcgtagagg aaaggaatac ttctttctaa gtttgggtct
     8161 atatcgctga ttcctttctc tatcttcttt cttatcgttc tttcggtgtc tagcttatct
     8221 atgcgaaatc taatttttat gatatctata ataggcaaat aggagattga ttttccatag
     8281 gaaatacatc taccctctag ataaagtatt ttctcatctt ctaggagttt cctaaattca
     8341 taaagaagtc gagatttacc tatacccgca tcacctatta tggttacaac ctgtcctaac
     8401 cct
//
LOCUS       DATDRL010000373         3384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2666261, whole genome shotgun sequence.
ACCESSION   DATDRL010000373 DATDRL010000000
VERSION     DATDRL010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2666261"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..1318
                     /locus_tag="VI935_12360"
     CDS             329..1318
                     /locus_tag="VI935_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26422.1"
                     /translation="MSKNLIINGLLKEDLETLASQIALWENMTIWNVAHKKLKGVYTS
                     IDEINKEIEEDKRTLFRKHKRNPSALEAKILKSLCDHLSIDTTKVKSIGDLVDLGEEI
                     EKRVILLQKKISKDKFRGDSIQELIQFNLKRMFDDLSKNFATKGVEDQKEIVKRIVKV
                     LEEMPDEQKEILRKKLNVDKITDQAVAKAIITGALGVGFAAVVEVAGFSAFIFAVEAL
                     AATAGLVGLTLPFAAYTTLTSIMAALANPFLIIPATLGLGYFLTERANKQIHDALLPI
                     LITQIAVSSSKEGNIISKDEKLIRKLKSISKKENTYFTKAGSWIKSLRKRSKK"
     gene            1315..2637
                     /locus_tag="VI935_12365"
     CDS             1315..2637
                     /locus_tag="VI935_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26423.1"
                     /translation="MKDINLTGRIDELLSKSKNLGEILDAPVSPYTQEEKLLSILKVS
                     DEGKTDIAFAGLTIGDFIYDALRVDPMVIEAVDFARAEDVGNIFKFAVYSDILEDLSI
                     TTLEGNIHNLKGYVAERFVAQELQSEGMEVEFPKDPNQEGFDLLVNGDPFQVKCLADQ
                     TGVLEHIERFPNIPVLINEELANSLEDVPNVYPVPGLTNEGIETATRDSIEAGEEILD
                     YEIPFIAISVAIGKNVFSILNGKTDLKHGSINVAYDVAGGIAGGELGSSGLALCGSLL
                     GPYGTVVGGITGAIIGSMYGRRIVSKIKRKIHTLKEEELAESALREFVIKSYVSSKKS
                     QKIFEKKAKLLLDGLLEKGNDMEFLTNYVKKRLNHERRYLKDKIQQLSKFRKYPRSID
                     PQTNDILVAGINAITLSLRAKVHPYSVKEPMDDLMDSLKTVQEKKAKL"
     gene            2967..3200
                     /locus_tag="VI935_12370"
     CDS             2967..3200
                     /locus_tag="VI935_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26424.1"
                     /translation="MDKQFNGYTAMQTAEGFHVVFHDSSKTPLDVKFNDFQEFANKYS
                     QELLAGKIPKLSDQEAVLLSLWEMVLIPANTIH"
BASE COUNT         1155 a          529 c          683 g         1017 t
ORIGIN      
        1 aatatacaga attataaaat cgttaataac ttgagtcaga atttgaacca gtggggtcta
       61 gtctctatta aacttttctt cgttaataaa cttcttctat ttcttcgtat ttcctttact
      121 cctactaatg tcaaaagttg agaccccaga atcttcttgc ctgaatccga ggaggctgtt
      181 aacattttcg attggcaatt agcgattaga agcatggggg ctttcgatgt tgctaggctt
      241 atgagccgcc cctaaaacgt gctattatta agattagagt caaattgagg tattagaaat
      301 tgcaagaaaa tcttgaggtg ctttcaatat gtcaaagaac ctaattatta atggattact
      361 caaagaagat ttggaaactt tagcttccca gatcgctcta tgggaaaaca tgactatatg
      421 gaacgttgct cacaagaaat tgaagggcgt atatacctca attgatgaaa taaataaaga
      481 gatcgaagaa gacaaaagga ctctatttcg caagcataaa aggaatccat cagccttaga
      541 ggcaaaaatt ttgaagtccc tttgtgacca tttatctatc gatacaacaa aagttaagag
      601 cattggcgac ttagttgacc ttggagaaga aatcgaaaaa agagttattc tgttacagaa
      661 gaaaatatct aaggataaat tccggggaga ttcaatacag gaactaatcc agttcaacct
      721 aaaaaggatg ttcgatgatc tttctaaaaa ttttgcgaca aagggtgtgg aagatcagaa
      781 agaaatagtt aaaagaatcg taaaagtgct ggaagagatg cccgatgagc aaaaagagat
      841 cctacgaaaa aaacttaatg tcgacaaaat tacagatcaa gcagtagcta aagctattat
      901 tacaggagcc ttaggggtcg gttttgccgc tgtagttgag gttgccggtt tttccgcctt
      961 tatttttgca gttgaagcct tagctgctac agctggtcta gttggattga cattaccttt
     1021 tgctgcatat actacattga catctataat ggctgcttta gccaatccct ttttaatcat
     1081 tccagctact ttaggtcttg gatatttcct aacagaaagg gcaaataagc agattcatga
     1141 cgcattgtta ccaatattga taactcagat tgcggtttct tcatcgaaag aaggtaatat
     1201 tatttcaaaa gacgaaaaat tgataagaaa gttgaaatcg atatcaaaaa aggaaaatac
     1261 atattttact aaagctggaa gctggattaa gagtctaagg aagagaagta aaaaatgaaa
     1321 gacatcaatc ttacaggacg gatagatgaa ttattatcga agtcaaagaa tttaggagag
     1381 attttggatg ctccagttag tccatatact caagaagaaa aattgttatc tatattaaaa
     1441 gtctccgacg aaggtaaaac tgatatagca tttgctggct taacaattgg ggatttcatt
     1501 tatgacgctt tgagagtaga tcccatggtt atagaagcag tggattttgc tagagcggaa
     1561 gatgtgggaa atatatttaa atttgcggtt tattctgaca tattagaaga tctatctatt
     1621 actaccttag aaggaaacat tcataatcta aaagggtatg ttgccgaaag gtttgtagcc
     1681 caagaattac aatcagaagg aatggaagta gaatttccaa aggatcctaa tcaagaagga
     1741 tttgatttat tagttaatgg agatcccttt caagtaaaat gcttggctga tcagacaggt
     1801 gtcttggaac atattgaaag atttcccaac attccagttc ttataaatga ggaattagct
     1861 aactctttgg aagatgttcc aaatgtatac ccggttccgg gtctaactaa cgaaggtata
     1921 gaaactgcaa ctcgtgattc aatagaagcg ggtgaggaaa tccttgatta tgaaattcct
     1981 tttatagcta tcagtgtagc gattgggaag aatgtttttt caattcttaa cggtaaaaca
     2041 gatttaaagc atggttcaat taatgttgct tatgatgtag ccggtggaat tgcaggggga
     2101 gaattgggat cttctggatt agctttgtgt ggaagtcttc ttgggcctta tggaactgta
     2161 gttggaggaa taacaggtgc aattattgga agtatgtatg gaagacgtat agtttctaaa
     2221 attaaaagaa aaattcatac tcttaaagaa gaagagctag cggaatctgc acttcgagaa
     2281 ttcgttataa agtcttatgt gtctagcaag aaatcacaga agatatttga aaagaaagct
     2341 aagttattac ttgatggtct tcttgagaaa ggaaacgata tggagttcct taccaattac
     2401 gttaaaaagc gtcttaatca cgagaggaga tatcttaagg ataagattca acagctatcg
     2461 aaattcagaa aatatccaag gtccattgac ccacaaacta atgatatcct ggtggcaggt
     2521 attaatgcta taacactctc attaagagca aaggttcatc cttattcagt aaaagaacct
     2581 atggatgatt taatggacag tctgaagaca gttcaggaaa agaaagcgaa attatagcct
     2641 ttaaatatcc ttaagacaat cgtacgtatc ctgaagcgta aaatgtaacc gaaagagcat
     2701 tggttgtgca atgtaaaaaa gtaaacgaaa taaagaggct acaatcttgg actcatgtct
     2761 tggattgtga attattttta ttcactatct tggttattgt gctgtagtgc aaatccatag
     2821 ctctaccaat atctagaagc gtgtacacat aattcaagtg atccgcctgc ctaaaaaaat
     2881 tcttatcagc atgaactgga taaaaaaaat ttttcctctt taaaattgat atttagtgat
     2941 tatattcaaa gttaaggtga taagatatgg acaaacaatt taatggctat acagctatgc
     3001 aaactgcgga gggtttccac gtggtgtttc acgattcttc gaaaaccccg ctggatgtaa
     3061 aatttaacga ttttcaagaa ttcgctaata aatatagcca ggaattactg gctgggaaaa
     3121 tcccaaagct ctctgatcag gaagcagttt tgctttcgct atgggaaatg gtgcttatac
     3181 cagctaacac aatacattaa ggtaatgaga tctgggttct atccatattt atcgagattt
     3241 agatcaacct tatttaaact ggccttttct tgaatgggtt tgtaaataga tagcatccta
     3301 cgtcattatt actcttgaca ctttcgggat tgatgcatat cttatactca gcactcatct
     3361 gactagagtg ctaatcccat ttat
//
LOCUS       DATDRL010000374         2653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3865579, whole genome shotgun sequence.
ACCESSION   DATDRL010000374 DATDRL010000000
VERSION     DATDRL010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3865579"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /gene="rplS"
                     /locus_tag="VI935_12375"
     CDS             complement(<1..273)
                     /gene="rplS"
                     /locus_tag="VI935_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HLE26425.1"
                     /translation="MNVIDEIERANMRKGLPSFRSGDTVAVHYIIKEGDRDRIQVFEG
                     VVIAKKGGGSRETFTVRKISYGVGVERIFPLHSPLVKMIDVKRKGKV"
     gene            complement(296..1585)
                     /gene="trmD"
                     /locus_tag="VI935_12380"
     CDS             complement(296..1585)
                     /gene="trmD"
                     /locus_tag="VI935_12380"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HLE26426.1"
                     /translation="MRYDILTIFPEFFKSFFSVGILKKAEEKKLIKISIHDIRRFTAD
                     KHKTVDDNPYGGGSGMVIKPEPLGSALEEVKTSGLKSLVILTTPQGERFSDKISRELS
                     KYEQLILISGRYEGVDERVRELYVDREISIGDYVLTGGEYPVTIIIDAVSRFIPGVLG
                     NEASPYDDSFKEGLLEYPQYTRPETYKGKTVPEILLSGNHKEIEKWRRRESIKRTFFR
                     RPDLIDRTRLTLEDIKLINQLKKENPHYFKVYIALIHYPIYNKDLKIITTAFTNLDVH
                     DIARAGKTYGVKGFFLIHPVKEQRELVKRVIWHWMEGPGVSYNPTRNEALSLVRIKSS
                     IDEALKEIEKIEGLKPKIVVTDARLRENMIGYTELKERILLGKEPYLVLFGTGWGIAN
                     EIIESSDYVLKPVEGVTDYNHLSVRSAAAIILDRLLG"
     gene            complement(1662..2171)
                     /gene="rimM"
                     /locus_tag="VI935_12385"
     CDS             complement(1662..2171)
                     /gene="rimM"
                     /locus_tag="VI935_12385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HLE26427.1"
                     /translation="MGLIPFGKVSKTHGLSGEVRIFPFSRKVENLSTLERIFIQKKRE
                     EKPLEFKIVRKRPEKHSAIVGLEGIKSVDAAEELQGCIVLVEASDLPETEENEYYWFQ
                     LVGLRVYTTEDKYVGKVEDLIESASQSLLVVKDERKEHLIPMINTIIREINLKHARIV
                     IFPLKGLLD"
     gene            complement(2194..2433)
                     /locus_tag="VI935_12390"
     CDS             complement(2194..2433)
                     /locus_tag="VI935_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HLE26428.1"
                     /translation="MEKLKELVSYIAKSLVDNPEKVEVREVAGEQTSVLELKVSQEDL
                     GKIIGKQGKTAKAIRIILSAAAAKIRKRAVLEILE"
     gene            complement(2497..>2653)
                     /gene="rpsP"
                     /locus_tag="VI935_12395"
     CDS             complement(2497..>2653)
                     /gene="rpsP"
                     /locus_tag="VI935_12395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HLE26429.1"
                     /translation="PRDGKFLEILGYFDPRKSPQTLQVNIERVKSWIERGAKPTERVE
                     KLLSKLM"
BASE COUNT          682 a          604 c          422 g          945 t
ORIGIN      
        1 tacctttcct tttcttttaa catctatcat tttcactaga ggcgaatgta gagggaaaat
       61 tctttcaact cctacaccat aagaaatctt tctcactgtg aaagtctccc ttgaaccgcc
      121 accttttttg gcaatcacaa caccttcaaa gacttgtatt ctatccctat ctccttcttt
      181 gattatgtaa tgcacagcta ccgtgtcacc tgaacgaaat gatggcaaac cctttctcat
      241 attagccctt tcaatttcat caataacatt catttttgtt tctccttttc atattttaac
      301 cgagtaacct atcgagtata atagccgcgg cacttcttac cgagagatga ttataatctg
      361 ttactccctc taccggctta agaacgtaat cagagctttc gatgatctca ttagcaatac
      421 cccagccagt gccgaatagc actagatagg gttcctttcc taaaagaatc ctttctttga
      481 gttctgtgta tcctatcata ttctctctta gcctcgcatc agtcacaact attttgggtt
      541 ttaacccctc tattttttca atctctttaa gagcctcatc aattgagctt tttatccgaa
      601 caaggctcag cgcctcattc cttgtagggt tataggaaac acctggtccc tccatccagt
      661 gccatatcac tcttttaact agctctcttt gctctttaac aggatgaata agaaaaaaac
      721 ctttcactcc ataagtcttg cccgcccttg ctatatcgtg aacatcgagg ttcgtaaaag
      781 cggtagtgat aattttaagg tctttattgt aaatgggata atgaattaag gcaatatata
      841 ctttgaaata atgtggattc tcttttttga gctgatttat taattttata tcttcgagag
      901 tgagtctagt tctatctatt aaatccggtc ttcgaaaaaa tgtccttttt atactttctc
      961 tccttctcca cttttcaatt tccttatgat ttcctgaaag tagaatttca ggaacggttt
     1021 ttcctttgta ggtctcgggt cttgtgtact gtgggtattc tagaagccct tctttaaatg
     1081 aatcatcgta gggtgatgcc tcgtttccga gaacaccagg aataaatctt gatactgcat
     1141 ctataattat agtgacaggg tattctcctc ctgtaagaac ataatcacca attgaaattt
     1201 ccctatctac gtatagttct ctaactcttt catccacccc ctcatacctt ccggagatta
     1261 agattagttg ttcatattta gaaagctctc ttgatatttt atctgagaac ctttctcctt
     1321 gcggtgtagt aagaataaca agagatttta aaccacttgt ttttacttct tctaaagcac
     1381 tgccaagagg ctcaggtttt attaccatac cgcttccgcc gccgtatggg ttatcatcta
     1441 ctgttttgtg tttatccgca gtgaaccttc ttatgtcgtg gatgcttatt tttataagct
     1501 ttttctcctc ggcttttttt agaattccaa cggaaaagaa ggatttaaag aattctggga
     1561 aaattgtaag aatatcgtat ctcatgaatt agccatcagt tatcagccgt cagtaaaggc
     1621 ttttgctgat ggctgacggc tgattactga ttgatattca tttaatctag aagtcctttc
     1681 aatggaaaga taactattcg tgcatgttta aggtttattt ctcttattat tgtattaatc
     1741 attgggatta ggtgttcctt cctttcatcc ttaactacaa gaaggctttg cgatgcactt
     1801 tcaatcaaat cttcaacctt acctacatat ttatcctcag tagtatagac cctaagtcca
     1861 actagttgaa accagtaata ttcattctct tctgtttctg gaaggtcgga ggcctcaacc
     1921 aatacaatac atccttgaag ttcctctgcg gcatcaactg atttaatgcc ctcaagccct
     1981 actatggctg agtgtttctc tggccttttt cttacgattt taaactcgag gggtttttct
     2041 tcccgttttt tttgtataaa tattctttct agtgttgaaa ggttttctac ctttcggcta
     2101 aacgggaata ttctaacttc cccagaaagc ccatgtgttt ttgaaacctt tccaaagggg
     2161 atgagaccca attccgcctt attgaaaggt ttattactca agaatctcaa gaaccgcgcg
     2221 ctttcttatc ttggcagcag cagcgcttag tataatcctt attgctttag cagtttttcc
     2281 ctgctttcca ataatcttcc ctaaatcctc ttgggaaacc ttcaactcta acacagatgt
     2341 ctgctcacca gcgacttccc taacctcaac cttctcaggg ttatcaacaa gcgatttcgc
     2401 gatgtagctc acaagctcct ttagcttctc catatcgttt acctcctggt ttttagaaac
     2461 cttcttcagc tctgacgtct gattctaact cagttcttac atgagttttg aaagaagttt
     2521 ctctaccctt tctgtaggtt ttgcaccacg ctctatccaa ctctttaccc tttcgatatt
     2581 tacctgaagt gtttgagggc tctttcgtgg gtcaaaataa cctaaaattt ccaaaaactt
     2641 tccatcacgg gga
//
LOCUS       DATDRL010000375         3829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3634369, whole genome shotgun sequence.
ACCESSION   DATDRL010000375 DATDRL010000000
VERSION     DATDRL010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3634369"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..1409
                     /gene="metK"
                     /locus_tag="VI935_12400"
     CDS             249..1409
                     /gene="metK"
                     /locus_tag="VI935_12400"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HLE26430.1"
                     /translation="MALGDFVFTSESVTEGHPDKMADQISDAVLDAIIIKDPPARVAC
                     ESLLTTGLIVIAGEITTNANIDFPGVVRRTVKDIGYTDSSMGLDADSCGVIVAIGKQS
                     PDIAIGVNPSEEKEQGAGDQGLMFGYAIDETPELMPAPIMYSHKLARRLSEVRKKGTL
                     PFLRPDGKTQVTMRYKDQKPVSVDAIVISAQHSPEVSNHTIYEGIIEEVIKPVIPGDL
                     LSTKTKIYINPTGRFVIGGPRGDTGLTGRKIIIDTYGGWGRHGGGAFSGKDPSKVDRS
                     VSYMARYIAKNIVASGIAKRCEVQIAYAIGIAEPVSIMVDTFDTGVVPDDKISKAIRE
                     VFELKPAEVIKSLNLLRPIYQKTAAYGHFGREEQEFTWERTDKIEQLRSAVE"
     gene            1478..2734
                     /gene="ahcY"
                     /locus_tag="VI935_12405"
     CDS             1478..2734
                     /gene="ahcY"
                     /locus_tag="VI935_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HLE26431.1"
                     /translation="MKYDVKDPTLAAHGKLRVEWAAEEMPVLAQIQDRFKKEKPLKGI
                     RIAACLHITTETVNLAITLKAGGAELALCASNPLSTQDDAAACLVKAHRISVFAIKGE
                     DTKTYYRHIESVLDVAPHITMDDGADLVSTIHKSRTELIDNIVGGTEETTTGVIRLRS
                     MAERGVLRYPIIAVNDAQTKHLFDNRYGTGQSTVDGVIRATNRLIAGTNFVICGYGWC
                     GKGVALRTRGMGANVIVTEVDPLRALEAVMDGLRVLSMDQAAKIGDFFCTVTGNTGVI
                     RKEHFIKMKDGAIICNAGHFNVEIDLKGLRGISRGRRKLREYVEEYTLSNGRRINVLA
                     EGRLVNLSSAEGHPSSVMDMSFANQALCVEYIVKNAKKMDNKVYDVPEKVDKTVAKMK
                     LRALGIKIDQLTQEQRNYLHSWEMGT"
     gene            2810..3697
                     /gene="xerD"
                     /locus_tag="VI935_12410"
     CDS             2810..3697
                     /gene="xerD"
                     /locus_tag="VI935_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HLE26432.1"
                     /translation="MDQFIDSFLTYLTVVKGLSKNTVESYQRDIQKLSLFLEKRGVSN
                     IEQVEYKHILDLLSDLKEKDLRARSVARALVSIRQFFKYLLIENIIKNDPTFLIRTPK
                     TKRTLPQVLSIEEVERLLASPDETTLKGLRDKAMIEILYATGIRVSELVGLGLNDVNF
                     ELGYLVAYGKGSKERIVPIGEKAKNKLREYLDAGRPSLLKSKTSVNLFITRLGKKMTR
                     QGFWKIIKSHTIRSGITKKISPHTLRHSFATHLLERGADLRAIQIMLGHSDISTTQIY
                     THIDRERLKEVHKKYHPRS"
BASE COUNT         1297 a          651 c          858 g         1023 t
ORIGIN      
        1 aattttgcat cttgttaatc aaatatccta tgcttttatg ttattattta atgaaactat
       61 attagggata actgccttat tgtattgtta tattatctag tgtttataat tactttgaat
      121 atagcatgtc acttgacata cgaacttaaa gatatatagt ttttccgctt ttatacgaac
      181 taaactaggt ataatcaaaa ttgaaataaa tactatttaa gaaatggcaa gtataggagg
      241 catgttatat ggcattaggc gattttgtat ttacctctga atctgttact gaaggccatc
      301 cagataagat ggcagaccag atttctgatg cggttttgga tgcaataatt attaaggatc
      361 ctccggcaag ggttgcctgt gaatctcttc ttacaactgg acttattgtc attgcaggag
      421 aaattacaac caacgctaat atagactttc caggtgttgt tagacgtact gtaaaagaca
      481 ttggctatac agatagctcc atgggattgg atgccgacag ttgcggcgtc atagttgcga
      541 ttggaaagca gtctcctgac attgctattg gagtaaaccc ctcggaagaa aaggaacagg
      601 gcgcaggaga ccaagggctt atgttcggct acgcaataga cgaaacccct gagctcatgc
      661 ctgcacctat aatgtattcg cataagcttg caaggcggct ttcagaggta aggaaaaaag
      721 gaactcttcc gtttcttagg cctgatggaa aaactcaggt gacgatgaga tataaagacc
      781 aaaaaccggt aagcgttgat gctattgtta tctctgctca acactcccct gaagttagca
      841 atcatactat ttatgaaggg ataatagaag aagtaataaa acctgtaatt cctggagacc
      901 ttctatctac aaagacaaaa atctatatta accctacagg aaggtttgta attggtgggc
      961 ctagaggtga tacaggttta actgggcgaa aaattataat agatacgtat ggcggatggg
     1021 ggagacatgg gggtggggca ttttctggaa aggacccgtc aaaggtagat agaagcgtca
     1081 gctacatggc acgctatata gcaaagaata ttgtggcttc ggggattgca aaaaggtgtg
     1141 aggttcaaat cgcttacgct atcggcattg cagaacctgt atctataatg gtagatactt
     1201 ttgatactgg agtagttcca gatgataaaa tttcaaaagc cataagagag gtatttgagc
     1261 taaaacctgc cgaggttata aaatcgttaa accttcttag gccgatttat caaaaaaccg
     1321 ctgcatatgg acattttggt agagaagaac aagaattcac atgggaaagg acagacaaaa
     1381 ttgagcaact tcgatctgca gttgagtaag ccagatcata tatattagat attaccaaat
     1441 actcatcagc aacacataat caaaaacggg ggtagagatg aaatacgatg taaaagaccc
     1501 aactcttgcg gcacacggta aactcagagt tgagtgggcg gcagaagaaa tgccagttct
     1561 tgcccaaata caggatagat tcaaaaagga aaaacctttg aaaggtataa ggatcgcagc
     1621 atgcttacat ataacgacag aaactgttaa tcttgctatc acattaaagg caggcggtgc
     1681 tgaactggca ctttgtgcat caaacccact cagcacacag gatgatgctg cggcatgtct
     1741 ggtaaaagct cataggatct cggttttcgc aataaagggt gaagatacta aaacatacta
     1801 caggcacata gaaagtgttc ttgatgtagc gccccatata actatggatg atggagcaga
     1861 tttggtttca actatacata aaagtaggac cgaactcata gacaatattg taggtggaac
     1921 agaagagacg acaacaggcg taatacgttt gagaagcatg gctgaacgag gcgtgctcag
     1981 gtacccgatt attgctgtaa acgatgccca aacaaagcat cttttcgaca atcgctacgg
     2041 aactggtcaa agcacagttg atggagttat tagagcaaca aataggctta ttgcaggaac
     2101 aaactttgtg atttgtgggt atgggtggtg tggaaaggga gtggctctga gaacacgtgg
     2161 aatgggtgca aatgtaattg taacggaggt tgatccactg agagcgcttg aagccgtaat
     2221 ggatggatta cgggtcctgt caatggatca agcagcaaag attggagatt tcttttgtac
     2281 agttaccggt aatacgggcg tcataagaaa agagcacttt attaagatga aagatggggc
     2341 aattatctgt aacgctggcc actttaatgt tgagattgat ttaaaggggc ttcgtggtat
     2401 ttcaagagga aggagaaaat taagggagta cgttgaagaa tacacacttt caaacggaag
     2461 aagaataaat gttcttgctg agggaaggct cgtcaacctc tcatcagccg agggtcatcc
     2521 atcaagtgtt atggatatga gctttgcaaa ccaagccctt tgtgtagagt atatagtcaa
     2581 aaatgcgaaa aagatggata acaaggtata tgacgtccca gaaaaggtag ataagactgt
     2641 tgctaagatg aaattaaggg cccttggaat aaaaattgat caacttacac aagaacaaag
     2701 gaattacctg cactcatggg agatgggtac ttagtatagg gtcaaatata ttagtcgtta
     2761 gtcactcgac aatgtaaaga caaataacga tcgactaatg acagatttta tggaccaatt
     2821 tatagactcg tttcttacct accttactgt tgtcaaaggg ctttctaaga ataccgtaga
     2881 atcatatcaa agggatatac aaaaactctc tttgtttctt gaaaagaggg gtgtcagtaa
     2941 tattgaacaa gtggaatata aacacatact cgatctcctt tccgatttga aggaaaaaga
     3001 cttaagagct aggtctgtag caagggcttt agtatctata agacagttct ttaagtatct
     3061 tcttattgaa aatattatta agaatgatcc tacgtttcta atacgcacac ctaaaacgaa
     3121 gagaactctc cctcaggtac tctcaataga agaggtggag aggcttcttg cttctccaga
     3181 tgaaacaacc cttaaaggcc taagggataa agctatgata gagattcttt atgcaacagg
     3241 cattagggta tcagagcttg taggactagg acttaatgat gtaaactttg agttaggcta
     3301 ccttgtagct tatggaaagg gctcaaaaga gaggatagtt cctattggag aaaaggctaa
     3361 gaacaaattg agagagtact tagatgcagg aaggccaagt cttcttaaat ccaaaacctc
     3421 agttaatctc tttattacta gattaggcaa aaagatgacg cgtcagggtt tctggaagat
     3481 aatcaagagc catacaataa gatcaggcat aacgaagaaa ataagcccac ataccctcag
     3541 acattcattt gcaactcatc tactcgaacg aggcgcagat ttacgggcta tccagataat
     3601 gctaggccat tcagatatat ccacaacaca aatttacacc catattgata gggaaaggct
     3661 caaagaggtg cataaaaaat atcatccgag atcataaaat attggtatct gataatttgg
     3721 ctaattcaaa agtcaatatc aaaaagccta aacttggata tcagacgcta ggtagtgtta
     3781 atttgagagc gtacataata agataataaa tatatggatt ctaattatt
//
LOCUS       DATDRL010000376        10577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1091374, whole genome shotgun sequence.
ACCESSION   DATDRL010000376 DATDRL010000000
VERSION     DATDRL010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10577
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091374"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..582)
                     /locus_tag="VI935_12415"
     CDS             complement(97..582)
                     /locus_tag="VI935_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HLE26433.1"
                     /translation="MQTIWAPWRIEYLTKEREDGCIFCEKPKEKKDRRNLILYRGETG
                     FIIMNRYPYSNGHLMAVPYRHIRELTQLNQKERLELINLTIRCVEILKGFKTEGFNIG
                     MNIGKAAGAGIDDHIHFHVVPRWSGDTNFMPVIGNIKVIPEYLDQSYSKLTQQIKTTK
                     R"
     gene            complement(704..1582)
                     /gene="sppA"
                     /locus_tag="VI935_12420"
     CDS             complement(704..1582)
                     /gene="sppA"
                     /locus_tag="VI935_12420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HLE26434.1"
                     /translation="MRGLFTIILFLLFILFSFIVGIGVGLIFSREDGFPVGERVAVLE
                     VEGIILDSQPYLDTLSKIKKDDGIKAIVLRIDSPGGAVGPSQEIYSEILKLREKKPVI
                     ATLGSVGASGGYYIACAAQKILANPGTITGSIGVIAQFVSYEQLLKWAKLDVEVIKSG
                     EFKDVGSPFKKMTETEKQYMQQLIDNVYSQFKLAVSKARGIDSKEMDKIADGRIFTGE
                     QAKNLKLIDELGTLSDAVNLAGSLSGIKGEPNVSYYPKKKINFLDFILSKFETEIISG
                     LPLKERFGFFYLVDIM"
     gene            complement(1592..3271)
                     /locus_tag="VI935_12425"
     CDS             complement(1592..3271)
                     /locus_tag="VI935_12425"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HLE26435.1"
                     /translation="MEEKNFSELLEESLRVPDRGEIFKGRVVRVDDEEVFIDFGFKSE
                     GIAPKVEFYGKNGSLKVNVGDEVEVILEKWRGEEGLPTLSKRRADLFKESERLEQSFA
                     SGKLVTAKIIEKVKGGLIADVGDDVEIRAFLPSSQIDLKPHGDLDQYIGKILEAKILK
                     LSNEGLVLSRKAYLEEQREIQRKRVLSSLKEGRIVSCKVIKIVDRGVIVDLVGVEGFI
                     PISELSWGRVKHPSDIVSVNEEIKVRVLRIDEDGKIYLGLKQIKPDPWNFTDIRYKPG
                     VKIRGKVVSTTDFGVFVELEPGVEGLVHVSEITWTKRFRHPKELVHVGSRVEVIVLEV
                     DVEKRRIGLSLRRIEPSPWELFKERNPSGTRIKGKIKNVTDKGIFVEVEEDLVGLVHP
                     SDISWKGRVSPRDMFTVGDTIDVSVIDVDVKNQRIKLGMKQLSKDPWEDALESYKPQE
                     TILSGKVTDVKEAGILVELENGLQGFIRASEFSREGSRELSKPIKIGDDLTALVMGLD
                     KPKRQVNLSKRKYEEWLEKERVSSFLSSQGEGSVKLGDVLGEKLKSIIKEV"
     gene            complement(3413..4264)
                     /gene="ispH"
                     /locus_tag="VI935_12430"
     CDS             complement(3413..4264)
                     /gene="ispH"
                     /locus_tag="VI935_12430"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HLE26436.1"
                     /translation="MSKKIILAETAGVCFGVERALKKSYEVTDKSEVDVYSLGPIIHN
                     PQVVRHLEEKGVKVIDKFEDAPKGTVMIRAHGVPLGTLSVAKDRGLNVVDLTCPIVKK
                     LQHSVKKLAEEAYFIVIVGDKKHPEIIGAVSYADSENVMVVESKSELEDQVFLKKRVG
                     IVAQTTIAFDRFREVVDEFLQRSVEEIKIFNTICDDIFNKQLEAVEIAKSVDVMIVIG
                     GKNSSNTTKIAKLCHEVNPNTYQIETADEVSSLCLNLHEKTIGVTAGASTPSWIIEEI
                     LTRLEKA"
     gene            complement(4261..4944)
                     /gene="cmk"
                     /locus_tag="VI935_12435"
     CDS             complement(4261..4944)
                     /gene="cmk"
                     /locus_tag="VI935_12435"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HLE26437.1"
                     /translation="MKRGIIITVDGPSGAGKGTLAQAIAKRLGYSYMDTGAMYRVIAL
                     LVHRENVNIDDKKRLSEILLQTHITFGRDGNSNLRVFLNREDVTEEIRTPVISKLSSE
                     IASNKVVRDYLRGMQRRIARKGNIVAEGRDMGTYVFPNADFKFYIDASLPERARRRWI
                     QLKESGIKVGIEEVKSDIERRDKQDKERVESPLHPAPDAVIIDTTNLTVDTAVEKIMK
                     TIMEGHNLK"
     gene            complement(5033..6322)
                     /gene="aroA"
                     /locus_tag="VI935_12440"
     CDS             complement(5033..6322)
                     /gene="aroA"
                     /locus_tag="VI935_12440"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HLE26438.1"
                     /translation="MSQENIKGDSIPLRGEFTPPGDKSISHRAVIIGSLAKGVTSVKG
                     FLTCGDTLSSAKAMRMLGIPIEMHESNVEIRGKGLLGLNEPEDTINAENSGTTARLLI
                     GLLSGQKFFATLTGDKYLRVRPMERVVKPLTLMGASIWGRDGGKRLPLAIQGTKLSGI
                     RYELPVASAQVKSALLLAGLYAKGETEVIEPTPTRDHTERMLSHFGVYIERNGSSVKT
                     RSGEDFKGVEISIPADISSAAFFIVAALINPGSEILIKNVGINPDRIGAIEILRQMGG
                     SIKVINKRDESGEPVGDILVKSSPLKAVEIKGEIIPKAIDELPVIAVAACFAEGETII
                     KDAKELRVKETDRIKAMTTELKKLGAEVEELDDGMVIMGKENLKGAKCSSWGDHRIAM
                     ALAVAGTRAKGTTVIDDGDCVSVSFPGFFDVMKRLRG"
     gene            6494..7432
                     /gene="cofD"
                     /locus_tag="VI935_12445"
     CDS             6494..7432
                     /gene="cofD"
                     /locus_tag="VI935_12445"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HLE26439.1"
                     /translation="MITALGGGVGAAKFLNGLSQILKDEPLSVIVNTGDDIVISGLRI
                     SPDIDTIIYRLSGVVDKEKGWGLEEDTFHCLEAVERFGLETWFKLGDSDLATHIYRTY
                     LRNSGLVPSEIATKIAKAFGINNIKIIPMTDEDVETWIVTDKGEMHFQEYLVKKKMTP
                     EVRGIFIKGIEKAGPAPGVIQAIEEASGIIVCPSNPIISIGPILQVKGIREALKRTRA
                     KVIAISPLVGGAPLKGPADKLMRGLGLEVSSAQIARLYVDFLDEIVIDEKDAYEAEEI
                     RSIGIKPVVADTVMSNDEKAKRLAGRVLELFNEVQV"
     gene            7845..8051
                     /locus_tag="VI935_12450"
     CDS             7845..8051
                     /locus_tag="VI935_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26440.1"
                     /translation="MEKVKLELKGVTVELTLEALAEGLRKLSNEELEELEMLLVLDEL
                     KKRSKEVKQGKYVHLSDLKSLKNV"
     gene            8058..8333
                     /locus_tag="VI935_12455"
     CDS             8058..8333
                     /locus_tag="VI935_12455"
                     /inference="COORDINATES: protein motif:HMM:NF027117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLE26441.1"
                     /translation="MSSQVDKELEQLPPEVRRIINDVHFPAITAEPYKAGERLSGLLK
                     GFWKRSFRRSRVDCRIAYEIDEKEKAVYILMVGKRERFYERLERRAR"
     gene            8364..9119
                     /gene="cofE"
                     /locus_tag="VI935_12460"
     CDS             8364..9119
                     /gene="cofE"
                     /locus_tag="VI935_12460"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HLE26442.1"
                     /translation="MTNSISIIPLLGIPEIKPGDDLVKMVIDAAFKQDVTIEDRDIFV
                     FAQKIVSKSEGRIVKINDVSPSPFAIETGKLLDKDPRIVEIILRESRRIVKMDRGRLI
                     VENTKGIVCANAGVDMSNVSGGMHATLLPKDPDESAKRFAEGFKKELGVDVAVIITDT
                     VGRPWRDGLVQVAIGCYGIRPLKDYRGRKDSKGYDLRATALAVADEISSASGLVMGKT
                     EGMPVAIVRGYEYEKSKEGVKLLLRDPDNDLFR"
     gene            9103..10401
                     /gene="cofG"
                     /locus_tag="VI935_12465"
     CDS             9103..10401
                     /gene="cofG"
                     /locus_tag="VI935_12465"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HLE26443.1"
                     /translation="MTSFVNGAQEFLGSERIREMFKHFGIDDASLRVPSARLEEILIK
                     INSGKTIDREGALFLMNLNREEIVFLVLAASEVRERGKNKTVTFSKNVFVPLTRLCRD
                     HCGYCTFRIEPEEGELFVPPDEVINAAREGASVGCKELLFVTGDKPELVYTVYRDALE
                     RLGHKTTAEYLVAMSEAVLKENIFPHTNLGIANHDELSALRESNASMGLMLENISPRL
                     LQKGEAHYRSPDKVPKLRIRTMEYAGELRIAWTSGILVGIGETLEERVDSLFAIKRLS
                     QDYGHIQEIIIQNFSPKPGIPMELHPVPTFLDMLKTVGISRLMFGEKMNIQVPPNLNP
                     KAFPLLVLAGANDLGGVSPMTIDYVNPEAPWPQVERMERVLNELELNLRERLPIYPEF
                     ISEEFLDKRVLERMTKVIDSSGYVAQEVQNGNGRGKTKNN"
     gene            10373..>10577
                     /locus_tag="VI935_12470"
     CDS             10373..>10577
                     /locus_tag="VI935_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26444.1"
                     /translation="MEEVKQKITENRSLALDSLLSQVNGRTAEIINKSFNGYEISVEE
                     GEHLFNIQDVDELSLLAIAADEMR"
BASE COUNT         2973 a         2266 c         2100 g         3238 t
ORIGIN      
        1 aaattttgta ctaaaaacct cctgattttg gatgattaga gtaagggcaa gtatgaagaa
       61 aagagctatg ataattatct ttgcaaattt catttctcac ctctttgttg tttttatttg
      121 ctgggtaagt ttagagtaac tctggtcaag gtattcagga attactttta tgtttccaat
      181 aactggcata aaattagtat ccccgctcca tctgggaacg acatgaaaat ggatatgatc
      241 gtcaatccca gctccggcag ctttaccgat attcattccg atattgaacc cttcagtttt
      301 aaaacccttc aatatctcca cacatctgat ggtaagattt attagttcta atctctcttt
      361 ttgattaagt tgtgtaagct cccttatgtg tctatacggt acagccatga ggtgaccgtt
      421 agaataaggg tagcggttca tgataataaa tccggtttca cctcgataaa gtataaggtt
      481 tcttctgtcc tttttttctt taggtttttc gcaaaagata cacccatctt ccctttcctt
      541 ggtcaggtat tcaatacgcc aaggagccca gattgtttgc atattgtttt tatcatttcc
      601 ttctggtgca caccgtttat taccttccga tgcagactgc gcatcgggta acacagcacc
      661 agatacttat tatttccctc tggtgcacac tgcgcaccag atactacata atatcaacga
      721 gatagaaaaa cccaaaccgc tcttttaatg gcaaaccgct aattatctca gtttcaaact
      781 ttgaaagaat aaaatcaaga aaatttattt tcttctttgg gtaataagaa acatttggct
      841 cgccctttat tccactcaag gatcctgcaa gattaaccgc atcgctaagg gtgcctaatt
      901 catcaataag ctttagattc ttagcctgct ctccggtaaa tattctccca tcagctatct
      961 tatccatctc cttagagtca atccctcttg cctttgagac ggcaagctta aactgagaat
     1021 aaacattgtc aattagttgc tgcatgtatt gcttttctgt ctcagtcatc tttttaaaag
     1081 gcgagcccac atccttaaac tctccactct ttattacctc aacatccagt ttagcccatt
     1141 taagaagctg ctcgtagctc acaaattggg cgattacacc tatactgcct gtaattgttc
     1201 caggatttgc aaggattttt tgagcggcgc atgcgatata atacccacct gaagcaccta
     1261 ctgaacctaa ggtagctatt acgggtttct tttctcttaa ttttaaaatc tcgctgtaga
     1321 tttcttgcga gggaccaact gcaccgcccg gagaatctat ccgtaaaaca atggccttaa
     1381 tcccgtcatc ctttttgatc ttagagaggg tatcaagata tggctgggaa tcaagaatta
     1441 tcccttcaac ttctagcacg gcaacccttt cgccaactgg gaaaccgtct tctcgagaga
     1501 atatcaaccc gacaccgatt ccaactataa aggaaaataa tataaacaaa aggaatagta
     1561 taattgtaaa taggcctctc aatactctgc ctcatacctc cttgattatg gattttaact
     1621 tttctcctaa cacgtctcca agctttacag aaccctctcc ttgagaagat aggaaactag
     1681 aaaccctctc tttctctagc cattcttcat acttcctctt gctgagatta acttgacgct
     1741 tcggcttatc aagccccatt accagtgctg tgagatcgtc ccctatttta atgggtttag
     1801 ataattccct tgacccttct cgactgaatt ccgaggctct tataaatcct tgtaacccgt
     1861 tttcaagctc gaccaatatt ccagcttctt ttacgtctgt tacctttcca cttaggatgg
     1921 tttcttgagg cttgtaactt tcaagggcgt cttcccacgg gtcttttgaa agttgcttca
     1981 ttcctagttt gattctttgg ttctttacat caacatcaat aacagaaacg tctattgtat
     2041 ctccgactgt gaacatatcc cttggactta ctctaccttt ccatgaaata tcggagggat
     2101 gaactaatcc taccaaatcc tcttcaacct caacgaatat acctttatca gttacgtttt
     2161 taatcttccc ttttattctt gttcctgatg gattcctttc tttgaataat tcccatgggc
     2221 ttggctcaat acgtcgtagg ctgagtccga ttcttctttt ctcaacatca acttctagca
     2281 cgatcacctc tacacgactc ccaacatgta ctaactcttt tggatgtctg aacctcttag
     2341 tccatgttat ctcagagacg tggactaagc cttcaacacc cggttcaagc tcaacaaaca
     2401 caccaaaatc cgttgttgaa actaccttac ccctaatctt tacccctggc ttatatctta
     2461 tatcagtaaa attccagggg tcaggtttta tctgtttgag tccgagatag attttcccat
     2521 cttcatcaat ccttaatact ctaaccttta tttcttcatt taccgataca atatcactcg
     2581 gatgttttac ccttccccac gatagctcac ttattggtat gaatccttca actccaacca
     2641 ggtcaacaat tactccacgg tcgacgattt ttataacctt gcatgaaacg attctcccct
     2701 cttttaggga tgaaaggacc ctctttcttt gtatctccct ttgttcctct aagtatgcct
     2761 ttcttgatag gaccagcccc tcgtttgata gttttaaaat ctttgcttcc agtattttcc
     2821 caatgtattg atcaagatca ccgtgaggtt taaggtctat ctgggacgaa ggaagaaagg
     2881 cgcgtatttc aacatcatcc ccaacatccg ctataagacc tccttttacc ttttcaatga
     2941 tttttgcagt gactaatttc ccgctagcga atgattgctc taacctttca gattccttaa
     3001 aaagatctgc tcttcttttc gacagcgtag gaagaccctc ttcgcccctc catttctcaa
     3061 gaatcacctc aacctcgtct ccaacgttaa ccttaaggct cccattcttt ccataaaatt
     3121 cgacttttgg tgcgatcccc tcggacttaa aaccaaaatc aataaatacc tcttcatcgt
     3181 caacgcgtac aacccttcct ttaaatatct cccctctatc tgggacgcga agactttctt
     3241 caagaagctc tgaaaaattc ttttcttcca tatgcatctc ttaattggtt atatctaacc
     3301 gcatagcctc cagtagttat cgttgcaaac gcttgatttg tgaatcgtga tttgttgttt
     3361 tggttcgagt cacgattcac aaatcacgag ccccgaatca taaactataa tcctaagcct
     3421 tctcgagtcg agtgagtatt tcttcaatga tccaggacgg ggttgaggca cctgcagtaa
     3481 ccccgatcgt tttttcatgc aggtttagac ataaggaaga aacctcatct gctgtttcta
     3541 tctgatacgt atttgggttt acttcatgac agagctttgc gatcttagtt gtatttgagc
     3601 tattctttcc gcctattaca atcatcacat ccactgattt tgctatctca acagcttcaa
     3661 gttgcttgtt aaaaatatca tcgcatatgg tattaaatat ctttatttct tctacacttc
     3721 tttgaagaaa ctcatccacc acctcacgga atctgtcaaa ggcaatagta gtttgcgcga
     3781 cgatcccaac ccttttcttc aaaaatacct gatcttctaa ttcagatttg ctctctacta
     3841 ccataacgtt ttcagagtct gcgtagctta cagcgcctat aatttcggga tgctttttat
     3901 caccgactat aactataaaa tatgcctcct cagcgagttt cttcacagag tgttgaagct
     3961 tcttaacgat tgggcatgta aggtcaacga cattaagtcc tctatctttt gctacgctta
     4021 gagttcctaa aggcacgcca tgtgcacgaa tcatgacagt acccttgggt gcgtcttcaa
     4081 atttgtctat gacctttaca cccttttctt caaggtgcct tacaacctgg ggattatgga
     4141 taatgggacc aagtgaatat acatctactt ctgatttatc cgttacctca taagattttt
     4201 ttaaggccct ttccactcca aagcataccc cggcagtctc tgccaatatg atttttttac
     4261 tcatttaaga ttatgaccct ccataatggt tttcattatc ttttctaccg ctgtgtcaac
     4321 cgtaagatta gttgtatcta ttatgactgc atcgggagca ggatgcaagg gtgactcaac
     4381 gcgttcttta tcttgcttat cccttctttc tatatcgctt tttacctctt caatacctac
     4441 cttaattccg ctttccttta gttgaatcca cctccttctt gcacgttcgg gtaatgaagc
     4501 gtctatgtag aacttaaaat ctgcgttagg gaatacatag gttcccatat ctcttccctc
     4561 agctactata ttaccttttc gggcaattct cctttgcatt cccctaagat agtcccttac
     4621 caccttgtta gaagcaattt ctgaagagag tttggagatc actggagtgc ggatttcttc
     4681 tgtcacatcc tctctattta agaataccct gagatttgaa tttccgtctc ttccaaaagt
     4741 aatatgggtc tgtaacagta tttctgatag tctttttttg tcatcaatat tgacattctc
     4801 tctatgaact aacagtgcaa taactctata catagccccc gtatccatat atgaatatcc
     4861 aagccttttc gctatggctt gagcaagcgt accctttcct gcccccgaag gtccgtcaac
     4921 ggttattata atgcctcttt tcatcctaac tgtttagcca cggagaacac agagatcaca
     4981 ggtaggaaag ttttattctc tttgtactcc gtgacctctg tggctagttt tttcaccccc
     5041 ttagcctttt catcacatca aagaatccgg ggaatgatac agatacacaa tcgccatcat
     5101 caataacagt agtaccctta gcacgggttc ctgcgactgc aagggccatg gcaattctat
     5161 ggtctcccca actcgagcac tttgctcctt ttagattttc ctttcccata attaccatac
     5221 catcatctag ttcctcaacc tcagctccca gtttttttaa ctctgttgtc atagccttaa
     5281 tccggtctgt ttctttaact cttagttcct ttgcgtcttt tattatagtt tcaccctcag
     5341 cgaagcatgc agcaactgcg attacaggaa gctcatctat agccttagga attatctccc
     5401 cttttatctc cacagccttt aatgggcttg atttcaccaa tatgtccccc acaggttctc
     5461 cgctttcatc ccttttatta atgactttga tggaaccacc catttgtctt aatatttcta
     5521 tagcgccaat tctatctgga tttatcccga cattctttat taagatttct gaaccagggt
     5581 tgattagtgc ggcaactatg aaaaaggctg ctgaagagat gtcggcaggg attgatatct
     5641 caaccccttt aaaatcttct ccgcttctgg ttttaacgct agagccattt ctctcaatat
     5701 aaaccccaaa atgagaaagc atcctttctg tatggtctct agtaggtgtt ggctcaataa
     5761 cctcggtctc tccttttgca taaagcccgg caaggagaag agcggatttt acctgagcgc
     5821 ttgctactgg caactcatac cgtatgcctg acaacttcgt tccttgaata gccaagggaa
     5881 gccttttccc tccatctcgt ccccagatac tcgcccccat aagggttagg ggtttaacaa
     5941 ccctctccat gggtctaact ctcaaatatt tatcccctgt gagtgtggcg aagaattttt
     6001 gtccacttag tagtccgata agcagtcttg ctgtggttcc tgagttttct gcattaattg
     6061 tatcctcggg ttcattaagt ccaagaagcc ctttccccct gatttcaaca ttgctttcgt
     6121 gcatttcaat tgggattccg agcattctca tcgcctttgc gctggatagg gtatcgccgc
     6181 aggtaagaaa acccttgaca ctggttacac cttttgcaag ggaaccaatg ataactgctc
     6241 tatgagagat ggatttgtcc ccgggagggg tgaattctcc ccttaaagga atagaatcgc
     6301 ctttaatatt ttcttgagac acagaattaa aaattaccat ttcttgaaaa aaaggtaaaa
     6361 cgtgttaaca aacgatttgg cttgacagtc agtgtcacct agcataatct aaggctaaaa
     6421 ttggggatgg ggatagaata attccaaagt agatttcttg tcctataaaa aaaaactaaa
     6481 gaaggtctaa caaatgatta cagcccttgg cggaggggtt ggagccgcta agtttctgaa
     6541 tggtctttct cagatcctca aagatgagcc gcttagtgta atagtcaata cgggagacga
     6601 tatagtaata tctgggctaa ggatatctcc ggatattgac acaataattt acaggctttc
     6661 aggagttgtg gataaagaaa aagggtgggg gctagaagag gatacattcc actgtcttga
     6721 ggcagttgaa agattcggtc ttgaaacctg gtttaagtta ggagacagcg atctggcaac
     6781 ccatatttac cgaacatatt taagaaatag tggactagtt ccatctgaga ttgctaccaa
     6841 gatagctaag gcctttggaa tcaataatat aaaaattatc cctatgactg atgaggatgt
     6901 agagacatgg atagtgacag ataaagggga gatgcatttt caagagtacc ttgttaaaaa
     6961 gaagatgacc cctgaggtac gagggatatt cataaaagga atagagaaag ctggacctgc
     7021 tcctggggtc atccaggcga tagaggaggc aagtgggatc atcgtttgcc caagtaatcc
     7081 tatcattagt attggcccaa tattacaggt aaaaggaatt agagaagcgc ttaaaagaac
     7141 acgggcaaag gtcatcgcaa ttagccccct tgtaggtggg gcgcccttaa agggacctgc
     7201 agacaagctg atgaggggtt tagggttaga ggtttcatca gcacagatag caaggctata
     7261 tgtggatttt ttagatgaga ttgtaattga tgaaaaggat gcctatgagg cagaagagat
     7321 aaggtcaatc ggaataaaac ctgtggtagc tgatacagtc atgtcaaatg acgaaaaggc
     7381 caagaggctt gcggggaggg ttttggaact ttttaatgag gtacaagttt aattaatgac
     7441 ttagatgggg ctgtggattt ctatccccca tggatggaat tctaagtggt tcgagtgcca
     7501 accagcaaga ctggttggct acccattttt aattctataa tttgggccac gagttacctc
     7561 gactgagctc gggacaggct tggcgcgata ttatcgcggc tagaagccgc tcctacggat
     7621 tttttgtgcg ttaagaataa tacccacatt gtaggagcac catcccttcg atgctctcag
     7681 gacaggcttg gtgggacaat aattttctca tccctgtgta ttctttgagg tgggggaaat
     7741 ctagtttttg taaaatcgtg ttgttattcc taggctataa tagcacatcc atatttactt
     7801 ttcattcaaa aaggaataag attgaaataa tgggaaaggc agtaatggaa aaagttaaat
     7861 tggagctaaa aggagtaacg gtcgaactaa ctttagaagc ccttgccgag ggactcagaa
     7921 agctctctaa cgaagagttg gaagagcttg agatgctact tgttttggat gagctaaaga
     7981 aaagaagtaa agaggttaag caaggtaaat atgtacatct tagtgatcta aaatccctaa
     8041 agaatgtatg agcttagatt tcatctcagg tagataagga gttagagcag ttacctccgg
     8101 aagttcggag aattattaat gatgtgcatt ttcctgcaat tactgctgag ccatataaag
     8161 caggtgaaag gctttcggga ctacttaaag gtttttggaa acgaagtttt cgccgatcca
     8221 gagttgattg ccgaatagct tatgaaatag atgaaaagga aaaggctgtt tacattttaa
     8281 tggtaggaaa aagagagaga ttctacgagc gcttagaacg aagagcaaga taagtctata
     8341 cccaaggcct ctcgtatata taaatgacga attctatttc aatcatacca cttttgggaa
     8401 ttcctgagat taaaccagga gatgatctgg taaagatggt gattgatgct gcgtttaaac
     8461 aagatgtgac tatagaagac agagacatct ttgtatttgc tcaaaagata gtttctaaat
     8521 cagaagggcg gattgtaaag attaatgatg tttctccgtc tccttttgcc atagagaccg
     8581 gaaaactact tgacaaagac ccccgaattg tggaaataat actaagagaa tcccgaagaa
     8641 ttgttaaaat ggataggggt aggctaatcg tggaaaacac taaaggaatt gtttgtgcta
     8701 atgcgggagt tgacatgtcg aatgtaagtg gaggtatgca tgcaactcta cttccaaaag
     8761 accctgatga atctgcaaaa aggtttgcag aaggattcaa aaaagaacta ggagttgatg
     8821 tggcagttat aataacggat actgtaggaa gaccatggag agatgggctt gttcaggtag
     8881 caattggatg ctacggaatt aggccattaa aggactatag gggcaggaaa gattctaaag
     8941 ggtacgacct tcgtgcgaca gcgctcgctg tagcagacga aatctcctct gcttcaggac
     9001 ttgttatggg aaagactgaa ggaatgccag ttgcaatagt gaggggatat gagtacgaaa
     9061 agagtaaaga aggcgtaaaa ttgcttttga gggatccaga taatgacctc tttcgttaat
     9121 ggtgctcagg aatttttggg ctctgaaaga attagagaaa tgttcaagca ctttggtata
     9181 gatgatgctt cattaagagt tccatctgca agattagaag aaatactaat caaaataaac
     9241 tcaggtaaaa caatcgatag agaaggcgcc ctcttcctta tgaacctgaa tagagaagaa
     9301 atagtttttt tagttctcgc agcatcagaa gttagggaga gaggcaagaa taaaacggtt
     9361 acattctcta agaacgtgtt tgtacccctc acaagactat gtcgtgacca ctgtggttac
     9421 tgcacgttta ggattgagcc cgaggaagga gagctttttg ttcctccgga tgaggtcata
     9481 aatgcagcaa gggaaggggc aagtgttggt tgtaaagaat tactttttgt aactggagat
     9541 aagcctgagc ttgtctatac tgtctatcgt gatgcacttg aaagactagg gcataaaact
     9601 accgcagaat atttggtagc tatgtctgag gcagtactta aagagaatat ctttccacac
     9661 acaaatcttg gtattgcaaa tcatgatgaa ctctctgcac tcagagaatc aaacgcaagc
     9721 atggggctta tgcttgagaa cataagccca aggcttcttc agaagggtga ggcccactac
     9781 cgttctcccg acaaggtgcc taaactcagg attaggacaa tggagtatgc aggggagcta
     9841 aggattgcat ggacatctgg aatacttgta ggcataggag aaacacttga agagagggtg
     9901 gattcgcttt ttgctataaa gaggctctct caggattatg gccacattca ggagataatt
     9961 attcaaaact tcagcccaaa acctggaatt cctatggaac tgcaccctgt cccgactttt
    10021 cttgatatgc ttaaaactgt ggggatttca aggctaatgt ttggtgaaaa gatgaatatt
    10081 caagtacccc ctaatcttaa tcccaaagcg tttcctcttc ttgttctggc tggggctaac
    10141 gatttgggtg gggtatcccc aatgacgatt gattatgtaa atcctgaggc cccctggccc
    10201 caggtagaaa ggatggaaag ggtactaaat gagcttgagt taaaccttag agaaagactc
    10261 cctatttatc ctgagtttat tagtgaggag tttttggaca aaagggtttt agagcggatg
    10321 acaaaggtta ttgactcttc gggttatgta gcacaggagg tgcaaaatgg aaatggaaga
    10381 ggtaaaacaa aaaataactg agaataggtc tcttgcactg gattcacttc tttctcaggt
    10441 taatggaaga acggcggaga tcattaataa atcctttaac ggatatgaaa tctctgtaga
    10501 ggaaggagaa caccttttta acatccagga tgtggatgag ctttccttac tcgctattgc
    10561 cgcagatgag atgaggc
//
LOCUS       DATDRL010000377         3156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3600829, whole genome shotgun sequence.
ACCESSION   DATDRL010000377 DATDRL010000000
VERSION     DATDRL010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3600829"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..824)
                     /locus_tag="VI935_12475"
     CDS             complement(39..824)
                     /locus_tag="VI935_12475"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HLE26445.1"
                     /translation="MKKFIPLLGLLIVFAWGCGKSEKKEETEKASTTVKEVTKSNKKI
                     VARVNGRPVYEEDLMGRPTKEVIVDEILYEEGLKRGLDKKFEERIERYRRSLIINELK
                     TEIIKTIPKKVDPKEIEGYYKENESRYKYLRVIEVSVEDKNLAEEIHQKTLEGQDLEK
                     IASDYTNSGKKVTVRDSGFRKNYHDIFGGKDIGSVSEVIQEGDGFKILKLVEVREVPL
                     SKLNKPIKSNLLAKKRAEAIQEFTEKLKEENKIKVEIIEEEGK"
     gene            complement(1174..1932)
                     /locus_tag="VI935_12480"
     CDS             complement(1174..1932)
                     /locus_tag="VI935_12480"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLE26446.1"
                     /translation="MRKLLPFLALLILFVWGCGKSEKKEEAQKSETTTDKTVVARVNE
                     RPIYENDLKGKPLEDVIDYEILYEVGLKQGLDKKVEKQLEDYRKRLIIAALQKDIMAN
                     LPKEEVSEQEIEEYYKQNVGKYKYMSFKEIVVIDQNIANEIHKRALKGEDFEKIASDY
                     SESGTNVQVRDLRFNKKYNERFIGKEIGSVSEIIQEGNNFVILKLTEVKEIPFNKTAP
                     AIKYSIIATKRAQAVHNFAEKAKNENNIKVEILK"
     gene            2342..2599
                     /locus_tag="VI935_12485"
                     /pseudo
     CDS             2342..2599
                     /locus_tag="VI935_12485"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            complement(2582..>3156)
                     /locus_tag="VI935_12490"
     CDS             complement(2582..>3156)
                     /locus_tag="VI935_12490"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HLE26447.1"
                     /translation="YEAGLKQGLDKKVEKAVEDYRKRLILTALQKEIMNNLPKEENVS
                     DEEIEKYYKENESKYRYLRIKEIAVEDKNLAEEIHKRALKGEDFEKIASDYSKSGTRI
                     TVRDLRFNMTYNDFFSGKDVGSVSEVVQEGNQFKILKLTEVKEIPHYKVMEAIRHTIL
                     AKRKAQAVHEFAEKFKNENKIEVEILEEKE"
BASE COUNT          796 a          660 c          497 g         1203 t
ORIGIN      
        1 gcacaagata tgacgataga taattagaga ggggggtttc attttccttc ctcttcaata
       61 atctccactt tgatcttgtt ttcttccttt agcttctccg taaattcttg tattgcttca
      121 gccctttttt ttgccaatag attagactta ataggtttat ttagtttgga aaggggaacc
      181 tcgcgcacct caacgagttt taggatctta aatccgtcac cttcctgaat gacctcactg
      241 acactgccta tatcttttcc accaaagata tcgtgataat ttttcctaaa acccgaatct
      301 cttacagtca cttttttgcc ggagttagta taatctgatg caattttttc tagatcttgt
      361 ccctcaagtg ttttttgatg tatttcctcg gcaagatttt tatcttctac agatacttct
      421 attacacgca ggtacttata tctactttca ttttccttat agtaaccctc gatctcttta
      481 gggtctacct ttttgggtat agtttttata atttcagtct taagttcatt tatgatcagg
      541 ctccttctat aacgctcaat tcgttcttca aattttttat caaggccacg tttcaaacct
      601 tcctcgtata gaatctcatc aactattacc tctttagtgg gtcttcccat taaatcctct
      661 tcatatacag gccttccatt aacacgggca acgatctttt tgttactttt agttacttct
      721 ttaactgtag tagaagcttt ttctgtttct tcttttttct ctgatttacc acagccccaa
      781 gcaaatacga taagtagacc taatagcggg ataaactttt tcatgtatct ggtccctcca
      841 tattgaattt taaggtaaac aatataactg gaaattgacg tagtgcaata cttgtctgga
      901 attaatatta agcccacttt tttaaaagag cttgagaaaa accagagata tgatacttac
      961 aatttaggcc gaggctttag gttttggcaa gtgactccat atttccttgc gtttcttagt
     1021 ttgataccaa aacccttcat taatctcaat agtgttatct taatgccttg ttcttgcaaa
     1081 aaccttagag gggagtgctt tgcaatcaga tcatccccca aaggttctaa tacaatatct
     1141 attatgaagc ttttattttt gacgcctttt cttctatttt aaaatttcta ccttaatatt
     1201 gttttcgttc ttcgctttct cagcaaaatt atgtacggct tgggcccttt ttgttgcaat
     1261 aatgctatat ttaatagccg gcgctgtttt attaaaaggt atctctttaa cttcggtgag
     1321 ctttaaaatt acaaagttgt ttccttcttg aatgatctca cttacactac ctatctcttt
     1381 tccaataaaa cgttcgttgt atttcttatt aaaccttaaa tcccttactt gaacattcgt
     1441 tcccgactcg gagtaatcag aagcgatttt ttcaaaatct tctcctttga gtgctctttt
     1501 atgtatttca tttgcgatat tttgatctat aacaactatt tctttaaaac tcatatactt
     1561 atatttaccc acattttgct tatagtattc ttcaatttct tgctcgctta cttcttcttt
     1621 tggcagatta gccataatat ctttctgtaa agcggctatt attaatctct ttctgtaatc
     1681 ttctaattgc ttttcgacct ttttatcgag accctgtttc aaaccgactt cgtataggat
     1741 ttcataatct atgacatctt caaggggttt tcccttcaag tcattctcat aaattggcct
     1801 ttcattaacc cgagcgacga ccgttttgtc ggtagtcgtt tcagattttt gggcttcttc
     1861 ttttttctct gattttccgc atccccaaac aaataaaata agtagagcta agaaggggag
     1921 aagctttctc atgtttctga ttcctccata atgaatttta ggataaataa tataaattga
     1981 aatttgatgg aatgcaaccc ttgcccagga ttaatattaa gctcacttat ggtttttttt
     2041 gtgcgatttt aattatactg tttaggtgaa gttgccttta gttcttcatt taaaaagcgt
     2101 tcaagaagaa aattaaagga ggtttgacat gaagcctttt cttacattaa ttcttatttt
     2161 agttttggta agtcttggac tgagtggttg aactcttgta ggggtttcct cccttaaagg
     2221 cgatataaaa agccagtgtg aaaaggtagc cggtgctttt ggagggagtt aaaaggatag
     2281 cctctcgttc tgccgctcct gctcagccga ccaaggttac tgtaagcata acgatgaccc
     2341 tgtaaagggc gacccaaagg cgcctatgac catagttgac ttcagcgact accagtgccc
     2401 gttttgtaga aggtttcacg agcaagttct gtcctccctt gagggtgagt atataaataa
     2461 aggaaatgta agatacatct ttatggactt cgccctggca tttcaccagc aggcactccc
     2521 tgctgctatt gccgcaaact gcgcaggcgc aggggaacag ggaaaatacc tgttgttggc
     2581 ttcattcctt ttcttctaaa atctctacct cgattttatt ttcattctta aatttctcag
     2641 caaattcgtg cacagcttga gcctttcttt ttgctaatat ggtatgcctt atagcctcca
     2701 ttactttgta atgcgggatc tcttttactt cagtgagctt taaaatctta aattggtttc
     2761 cttcctgaac gacttcactt acactaccta catcttttcc actaaagaag tcattatatg
     2821 tcatattaaa tctcaaatcc cttacggtga tcctcgttcc ggatttagaa taatcagatg
     2881 cgattttttc aaaatcctct cccttgagcg ctcttttatg tatttcttcg gcaagattct
     2941 tatcttcaac ggctatctct tttatacgta ggtacctgta tttactctcg ttttccttat
     3001 agtatttctc aatctcttcg tcgctgacgt tttcttcttt aggcaggtta ttcataattt
     3061 ctttctgtag agcggttaat attaatctct ttctatagtc ttctaccgcc ttttcaacct
     3121 ttttatcaag tccttgcttt aaccctgctt cgtata
//
LOCUS       DATDRL010000378         7231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_1641891, whole genome shotgun sequence.
ACCESSION   DATDRL010000378 DATDRL010000000
VERSION     DATDRL010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641891"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VI935_12495"
     CDS             <1..288
                     /locus_tag="VI935_12495"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HLE26448.1"
                     /translation="IGITWTTIFVLINILQPGSFYINPPNNIDNVQDWSDFIYYSYAT
                     LTTLGYGDITPVTSQARSVGVLETIVGQLYLTILLARLIGLHLTKSIKRES"
     gene            complement(327..605)
                     /locus_tag="VI935_12500"
     CDS             complement(327..605)
                     /locus_tag="VI935_12500"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HLE26449.1"
                     /translation="MTIQASLSGSVGHEADVATMRSSIVTYARPDKGLFAGVDLQGTS
                     ISYHGKTIEKVYGVLMGPYEALYKQQSFPATVRAFHDILTKFAPSQKN"
     gene            691..1488
                     /locus_tag="VI935_12505"
     CDS             691..1488
                     /locus_tag="VI935_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015124049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLE26450.1"
                     /translation="MPNKRIMGTANIKLDPTKRFSTRAQYYNKYRPNYPEAIIKLLKI
                     ECGLSPSSVIADIGSGTGILSCLFLKNRNIVFGVEPNKEMRGIANKFLKDYSNFKSVN
                     GSAESTNLKDQSVDFITSGQSFHWFDTKKSRKEFLRILKPDGWVVLIWNDRKTDATPF
                     LKAYDNLLYKFGIDYIDVSQQIPGVKVFTEFFGHDSFQLKIFHNLQIFNYERLKGRVL
                     SSSYTPMKGHPNFEPMMNELRRTFLEFQTNGKVRFDYDTKLYYGQFK"
     gene            1532..2362
                     /locus_tag="VI935_12510"
     CDS             1532..2362
                     /locus_tag="VI935_12510"
                     /inference="COORDINATES: protein motif:HMM:NF018963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase-like
                     domain-containing protein"
                     /protein_id="HLE26451.1"
                     /translation="MIKALKSLLIISFVVIIMEATSHASTSNLTDPTVANEELISLGK
                     WNEKQLDHIIRESAKIHELDKKIDFLSGQFLNVDYKESTLIGNIDTPEVFVINLRGVD
                     CFTYVDYIEAMRLSSSFLEFKDKLRTIRYQSGNINFLNRNHFFTDWEYFNSLYVVDVT
                     EKIGGDETKMVLKTLNDKNDGTYFLSGIPPKKRKIQYIPLDAIDDEMVSKLISGDYIG
                     IYSNQDGLDVSHVGIIIKEGDKVYFRHASSSKGNRKVVDEDFMNYISDTPGILILRPK
                     "
     gene            2484..3947
                     /locus_tag="VI935_12515"
     CDS             2484..3947
                     /locus_tag="VI935_12515"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HLE26452.1"
                     /translation="MDSIEALRSTLLIGPELALIITGIIVMVIDALIRGKAKTELFWI
                     ALLGLAVGFVLNLERFHQVSSAFAGALSLDEFAAYFNIIFIVGAFLAIVLSKDYIAKM
                     DLKANEYYALLLFSTSGMMILSSAKEFMSLFLGFEIMSISVYILSGFNRRSTRSSEAG
                     IKYLVLGGFASAILLYGIALIYGASGTIFLDEIAKRFDINNPLYLAGTALILVGFIFK
                     IGAFPLHQWVPDIYEGAPMAVTGFMSVGVKAAAFAILLRVLFEGFYQIETSWTQVLWV
                     VSVFTMTIGNLAALGQRSIKRMLAYSSIAHAGYVLVGVVAAYGEKKLGLSSVLYYLFS
                     YTFMNLGAFGVLTYLSMKGRECETLDDISGLWEKKPLTAVALGIFMFSLAGIPPTIGF
                     FAKYRVFLSGVRAEFYWLVVIGILNSVLSAYYYLRVLVYAYMREEKVEFPSFKPTSAI
                     ALTILTIGTIILGIFPFYSWDLALKAADSFLLALQAR"
     gene            4117..4683
                     /locus_tag="VI935_12520"
     CDS             4117..4683
                     /locus_tag="VI935_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26453.1"
                     /translation="MKREYNNIQALLKENLKIKEHVPTQNLMNELKDVIYKGYFTKDE
                     FLRMGMWKSPRPKNRYLSNSENKIIEISKKVFSTKFEKRKIELLTQLKGVSIPVASAI
                     LTLTDTKNYGVIDIRVWQVLYLYGSVKVKQTGRNFSFKNWYNYLMKLRYFAKKFGVKA
                     RDIERAIFLYHYKIQKGKLYDKGATALR"
     gene            4748..5806
                     /locus_tag="VI935_12525"
     CDS             4748..5806
                     /locus_tag="VI935_12525"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HLE26454.1"
                     /translation="MFDYQSLKKYLFKLDPETTHNIAEYALRTAKHFPFCLNLIKRKN
                     LITNDRLKQNIFSKDFTNPIGLAAGFDKNGTMIEAMPALGFGFVEVGTVTPEPQPGNQ
                     KPRLLRFPEQESLENSMGFNNDGMLTIQGRLRKVYPFTLPIGVNIGKNKTTPQEKAID
                     DYVNLIHGFKDVCDYLVINISSPNTPGLRDLQNEAFTSTIFSTAQQITSKPILLKISP
                     DLEASQAVNLSVIAVQNGAAGIIATNTSIDYTILTNAKKIGGISGRAIQDKSFYIFNE
                     IAKELYGKTLLISVGGIDSGAEAYRRIKAGASLVQVYTALIFHGPSLVRKMNEVILEL
                     LNKDGFDHISEAIGANRR"
     gene            6284..>7231
                     /locus_tag="VI935_12530"
     CDS             6284..>7231
                     /locus_tag="VI935_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HLE26455.1"
                     /translation="MPKSKTQSKEDSSRHIARETIAAFLFTIGVFSSLSLLFYSSKSG
                     LDVKGAMGTIGVFISSMIGKAFGLCSFVIPIILFYTAVVVFANRTGSRLYQKAICAFI
                     FLFASTTLFGLIFRNKNLLGYSPPGGWLGNLFSSLLRDKITGSFGSYIIVTILLLLSL
                     IIISNLSLSGTLNWLGSLGKVLTFTIERLISVFKFTLRQVEVMIENVYDLFSKPKPKI
                     SKESTQARPKPIRQVVEEELPPKLEPATLPPIIVEQQKEKSGDKPLFKPVFQANFKLP
                     STDLLDPKRESEIKIDKDVVYEKAKLIEEKLADFGVSGKV"
BASE COUNT         2388 a         1255 c         1334 g         2254 t
ORIGIN      
        1 attggtatta catggaccac catatttgtt ttgataaaca ttcttcaacc cgggtcattt
       61 tacattaacc caccaaataa tattgataac gttcaagact ggtccgattt tatctactat
      121 agctacgcga cattaactac cttgggatac ggtgatataa cacctgtaac ctctcaagca
      181 agatccgttg gagtacttga aactatagtc ggccaattat atttgactat attattagct
      241 cgactgatag gtctacacct tacaaaatca ataaaacggg aaagttaaca catgctaacc
      301 gttccgtgtt taggctattt tccttactaa tttttttgac tcggggcaaa cttggtaagg
      361 atatcgtgga aggctctgac agtagcagga aagctttgct gtttgtataa tgcttcgtac
      421 ggccccatta acacgccata caccttttca attgtcttcc catgatatga gatggaagtg
      481 ccttgaaggt ctacgccggc gaaaagcccc ttgtcgggtc ttgcatatgt aacaattgat
      541 gatcgcatcg tggctacatc ggcttcgtga ccaacactcc cggataaaga cgcttgaata
      601 gtaatctaca tgctcacaaa gtgtcaagaa atataggttt aggtaaactt ataaatatta
      661 ttgattaact cattaaagat atattcctaa atgcctaata aacgaataat gggaacggcg
      721 aatattaaat tagatccgac taaaaggttt tctactagag cccaatatta caacaaatac
      781 cgtcctaact atcctgaagc aatcattaaa ttactgaaaa tagagtgtgg tctttcgcca
      841 tcttcggtca ttgcggacat tggttcgggt actggaattt tatcttgtct gttcttaaaa
      901 aataggaata ttgtttttgg tgttgaacca aataaagaaa tgagagggat tgctaataaa
      961 ttcttaaaag actattctaa ttttaaaagt gtaaatggtt ctgccgagtc tacaaatcta
     1021 aaagatcaaa gtgtagattt cataacttct ggacaatcct ttcattggtt tgatacaaaa
     1081 aaatcaagaa aagaattttt acgaatccta aaacctgatg gttgggttgt acttatctgg
     1141 aatgacagaa aaactgatgc tacaccattt ttaaaggctt acgataactt actttataaa
     1201 ttcgggattg attacataga tgtaagtcag caaatcccag gtgtaaaagt atttactgag
     1261 ttctttggac atgattcctt tcagcttaag atttttcaca atttacagat tttcaactat
     1321 gaacgtttaa agggaagagt tttatcatcg tcttatacac caatgaaagg acatcctaac
     1381 tttgaaccta tgatgaacga actacgcaga acctttcttg aatttcaaac caacggcaaa
     1441 gttagatttg attacgacac aaaattgtat tatggacagt tcaaataatg aagaatcagc
     1501 tcagccaatt attaggattt ttagtaaagg catgataaaa gcattgaaat cactgttaat
     1561 tatatctttt gtcgtaataa ttatggaagc tacctcccat gcttctacat caaatctaac
     1621 tgatccaact gtagccaatg aggaattaat ctccctaggc aaatggaatg agaaacaact
     1681 ggatcatatc ataagagaat ccgctaagat tcatgaatta gataagaaaa tagacttcct
     1741 atcgggacaa tttttaaacg tggattataa ggaatcaact ttgattggaa acatagatac
     1801 acctgaagta tttgttataa atttacgagg agttgattgt tttacctatg tggattacat
     1861 cgaagcgatg cgtctttcaa gctcttttct tgagtttaaa gataaactaa ggactataag
     1921 ataccaatct ggcaatatta attttctaaa taggaatcac ttctttactg attgggaata
     1981 ttttaattca ctttatgttg tcgatgttac ggaaaagatt ggtggagatg agacaaaaat
     2041 ggttttgaaa acacttaatg ataaaaatga tggtacttat ttcctgtctg gaattcctcc
     2101 taaaaaacga aagattcaat atattccttt agatgccatt gatgatgaga tggtttcaaa
     2161 actaatatcc ggggattata tcgggatata ttcgaatcaa gatggactcg atgtttcaca
     2221 tgtaggaatc attattaaag aaggagataa ggtttatttt aggcatgctt catcatcaaa
     2281 ggggaataga aaggttgttg acgaggattt tatgaactat atctctgata caccaggcat
     2341 acttatttta agacctaaat aataaaaaaa gattccacac aatggccgca actatttgga
     2401 taaacttgac aacccccctg cccttaccaa tactagacta ctcaaactgt cacctgagtt
     2461 tccaataaat ccaaggatat tatatggatt caatcgaagc acttagaagt acgctcctta
     2521 taggccctga gcttgcttta ataattactg gcataattgt gatggtaatt gacgctttga
     2581 taagggggaa agcaaaaaca gagctatttt ggatagcact tcttggactt gcagtcggct
     2641 ttgtattaaa tcttgaaaga tttcatcagg tatcaagtgc gtttgccggg gctctttcat
     2701 tagatgagtt tgctgcatat ttcaacatta tttttattgt tggtgccttt cttgccattg
     2761 tactatcaaa ggattatatt gcaaaaatgg atttaaaggc taatgagtat tatgcccttc
     2821 ttctcttctc aacctccggg atgatgattc tcagttctgc aaaggaattc atgtctcttt
     2881 tccttggttt tgaaattatg tctatctcgg tatacatact ttctggtttt aatagaagat
     2941 caactcgttc atccgaggcc gggataaaat accttgtttt aggggggttt gcctcagcga
     3001 tacttcttta tggaatagca ttaatttatg gagcatccgg cacaatattt cttgacgaga
     3061 ttgcaaaaag atttgatata aataacccac tatacctcgc aggaactgct cttattctag
     3121 tagggttcat attcaaaatc ggcgctttcc cacttcatca atgggttcca gacatctacg
     3181 aaggagcacc tatggcagta accgggttta tgtctgtagg tgtaaaggcg gctgcatttg
     3241 caattcttct cagggttctt tttgaaggat tctatcaaat tgaaacaagt tggactcagg
     3301 ttctatgggt tgtttctgtt tttacaatga caataggaaa tcttgctgcg ctaggccaaa
     3361 gaagcattaa aagaatgctc gcttattcaa gtattgccca cgccggatat gtgcttgttg
     3421 gagttgtagc tgcctatgga gaaaaaaaac ttggtttaag cagtgtgctt tattacctat
     3481 tttcatatac attcatgaat cttggagcat ttggtgtatt aacctatctc tcaatgaagg
     3541 gaagagaatg tgaaacgctt gatgatatat cagggctctg ggaaaaaaag ccacttaccg
     3601 ctgtagccct tggaatattc atgttttctt tggctggtat tccaccaaca attggtttct
     3661 ttgccaaata cagagtattt ctctcaggtg tgagagctga gttctactgg ctagttgtaa
     3721 taggaatttt aaacagcgtt ctttctgctt attactacct tcgagtgctt gtctatgcct
     3781 atatgcgaga agagaaggta gagtttcctt cttttaaacc aacctccgca attgcactca
     3841 ccattctaac aataggaact attatcctag ggatctttcc tttttattcc tgggacctcg
     3901 ccctcaaagc cgcagactcg tttcttctgg ctctacaagc gagataacaa cttaatctca
     3961 agtaattaat cttttgaaat cagggagggt tatatgatat ttcagattat gtataaaatc
     4021 tatgtaacaa gaatgaaatg aaaagactaa atcatgaatt cggccaaagg atatcaaaat
     4081 actgatttga ataagttatc ttaagttatg atacaaatga aaagggaata taacaacatt
     4141 caggcattat taaaagaaaa tcttaaaatt aaagaacatg tcccgactca aaatctaatg
     4201 aatgaattaa aagatgtaat atacaaaggc tactttacaa aggatgaatt tttaagaatg
     4261 ggaatgtgga aaagtcctcg tcctaaaaat cggtatttat ctaattcaga aaacaagata
     4321 attgaaatat caaagaaagt tttttctacc aagtttgaaa agagaaaaat tgaactgtta
     4381 acacaactaa agggtgtttc tattcctgtt gcatccgcaa ttcttacttt aactgacacc
     4441 aagaattatg gagtaataga tattagagtc tggcaagttc tctatctata tggttcagta
     4501 aaagtaaagc aaacgggaag aaactttagt tttaagaatt ggtacaatta cttgatgaaa
     4561 cttcgttatt ttgcgaaaaa attcggggtt aaagcaagag atattgaaag ggcgattttc
     4621 ctttatcatt acaagatcca gaaaggcaaa ttatatgaca aaggtgctac agcattaaga
     4681 taatcagttt tctacttaga taacaatctg tctttaatta gtaaagaaaa aacggatgtg
     4741 ccacaggatg tttgactacc aatcattaaa aaaatatctc ttcaaattag acccggaaac
     4801 aactcacaac atagcagagt atgctttaag aaccgctaag catttcccct tttgtttaaa
     4861 tctcataaaa agaaaaaacc ttattacaaa cgataggcta aagcagaata tttttagcaa
     4921 agatttcacg aatcctattg gcttagctgc gggttttgat aaaaatggga ctatgattga
     4981 ggcaatgcct gcacttggtt tcggttttgt tgaagttggc accgtcacac cagaacctca
     5041 accaggaaac caaaagccaa ggcttttaag atttcctgaa caagaaagcc tggaaaactc
     5101 aatgggtttt aataatgacg ggatgttaac aattcaagga agacttcgta aggtttatcc
     5161 atttactttg ccaatcggag tcaatatcgg aaaaaataaa acgactcctc aagaaaaagc
     5221 aatagatgat tacgtaaatc taatccacgg ttttaaggat gtatgtgact atctagttat
     5281 aaacatatca tctccaaata cgcctggctt aagagacctt caaaacgagg cttttacaag
     5341 tacgattttc agtactgcac aacagataac ctcaaaacct atcttactaa aaatctcccc
     5401 cgatttggaa gcttcccaag ccgtgaattt atctgtaatc gctgttcaaa atggagcagc
     5461 agggattata gcaacaaata caagtataga ttatacaatt cttacaaatg ctaaaaaaat
     5521 cgggggaata agcgggagag caatccaaga taagagtttt tatattttta acgaaattgc
     5581 taaggagctt tatggaaaaa ccttgctgat tagtgttgga ggtatagatt caggtgccga
     5641 agcttataga agaattaaag ccggtgcatc tttggttcag gtgtataccg cacttatatt
     5701 ccatgggcca tctcttgtta ggaaaatgaa cgaagtaata cttgaacttt tgaataaaga
     5761 cggatttgac catataagcg aagctattgg tgcaaatagg agataattaa ataatgatga
     5821 aaaaactaag ctgatgctta taataatgct gatttatatt tttagattaa tgaaatgacc
     5881 tgtagtaagc taaaggatat cagcattcaa ccagattttt ccttttgcca ttgcaaacaa
     5941 tctcaaaaaa attagatcgc ttcgctggga tatgtgatag cgatcaagag gggggaagtc
     6001 agggaaccgt agtgcttcac gatgacagtg gaaactctgt agcaagcaaa agaaaatagt
     6061 cgggttaaat cattttatta attctaaaac ccaaacccac aatcctaaga tgaaatctcc
     6121 tctctctttt cagcccataa gcaaaactat ggaagtccta ctttttattt gacaatttct
     6181 attaactccg ctattttgat aatcagttta tgcaagacgc agtgcttagg ctctctttga
     6241 ttggtatagt ttttatgtaa aagactatca aaataaggta gaaatgccta aaagcaaaac
     6301 tcagtcaaaa gaagattcct ctagacatat tgcaagagaa actatcgctg catttttatt
     6361 taccataggt gtattctcat cccttagtct cctattttac agttctaagt ctggacttga
     6421 tgtaaaaggc gccatgggga caataggagt ttttatctct agtatgatag ggaaggcttt
     6481 tgggctttgt tcctttgtga ttccgatcat tttgttttat acagcagtag ttgtttttgc
     6541 gaatagaaca ggaagcaggc tttatcaaaa agctatttgt gcatttattt tcctatttgc
     6601 ttctactaca ttatttggtc ttatatttag aaacaaaaac ctcttaggtt atagccctcc
     6661 tgggggatgg ctaggtaatc tattctcctc gctacttaga gataaaatca caggttcctt
     6721 tggatcatac ataattgtaa ctattcttct tctattaagt ttaattataa tatcgaatct
     6781 cagcctgtct gggaccctta attggttagg ttcactaggc aaggttttaa ctttcacaat
     6841 cgaaaggcta atttctgtct tcaaatttac actgcgtcaa gtggaagtga tgatcgaaaa
     6901 tgtttatgat ctcttttcaa aaccgaagcc aaaaattagt aaagagtcta cacaagcaag
     6961 acctaaacca attagacaag ttgttgagga ggaactgcca ccaaaactcg agcctgcaac
     7021 tttaccccca attatagttg agcagcaaaa agaaaaatcg ggcgataaac cattgttcaa
     7081 accagtattc caagcgaact tcaaactccc ttctactgat ttacttgatc cgaaaagaga
     7141 gtcagaaatt aaaattgata aagatgtcgt ttatgaaaaa gctaaactca ttgaggaaaa
     7201 gcttgccgac tttggagtat cagggaaggt t
//
LOCUS       DATDRL010000379         3469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_3601504, whole genome shotgun sequence.
ACCESSION   DATDRL010000379 DATDRL010000000
VERSION     DATDRL010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3601504"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VI935_12535"
     CDS             complement(<1..205)
                     /locus_tag="VI935_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26456.1"
                     /translation="MQKYTLSSASQSIKIVTTIFLLFMLIGIATNLLISYAKTGFSYN
                     GLIEYYRGVESGYPKTYLELLETT"
     gene            complement(226..1218)
                     /locus_tag="VI935_12540"
     CDS             complement(226..1218)
                     /locus_tag="VI935_12540"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HLE26457.1"
                     /translation="MKLIPIIAIFLALPQVIVERAFFVMGTMLEFRLYCENKEACDKA
                     ILKAYSEVKRLDDIFSNYKNNSVLSRVNSFAGDGRISVPPEFVKLTSRAIFFSGLTDG
                     AFDITVGKAIEIWKVSEEKNLMPNRDELQRAQDCIGFDKIKLYPREKQIELQSPCLSL
                     DFGGIGKGYALDRAVKILRSYGIKRGIVNFGGNIYAMQPPPGEDGWDVGIRHPRDEAE
                     VLTLLKVEDIAVSTSGDYERYFEVDGRRFSHIIDPRNGLPVESVPSVTVIAHDATDAD
                     ALSTALSVMKRDNAIKFIERLKDVGVMIVREKKGKLCIYKNPSYSKLEVIKTIN"
     gene            complement(1363..1941)
                     /locus_tag="VI935_12545"
     CDS             complement(1363..1941)
                     /locus_tag="VI935_12545"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HLE26458.1"
                     /translation="MKSRISAFAFTFFLVLVLRCLAISADKIYLSEEEAPKMVFPEAD
                     EFEIRVVESTPELREKVKGLIGKVEPSIWEKEYKTFIAKNDSKVIGYAVIVDEIGKHR
                     PITFIVGVTPEGKAENIAVMVYREPYGSEIRAKRFLKQYEGKTLDDPIRTRKDIVNIS
                     GATLSVRAANVGVKKALALIGVVYLQGENAVE"
     gene            complement(2237..>3469)
                     /locus_tag="VI935_12550"
     CDS             complement(2237..>3469)
                     /locus_tag="VI935_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26459.1"
                     /translation="NTFTLRRLVLTADARPVDRISIYTELEFERFLQIEVEESTTSQE
                     GGLTFEQEIESSNESEIALEQAWLQFDYAENHGLRLGAILVPLGRFNLYHDDNLYDFN
                     RRTITDRQGPVLPVAAAWDELGAGLNGNFDLGDKGGLNYQFYVMNGATLEGQIETLIE
                     TTPDESKIELEGTLDLSPGGFSADLNGNKAFTGRIAYSPFLGAEVGGSFYVGQYTPDF
                     LGINKYITAFGGDTKFSIGPFDIEAEYIYTHFEDAQQVVERFAELTVNSEVEGEIDDL
                     IVELAFESPMIAKNKQGFWLSLTYHFWPEFLNKTPLGSPFENPDLRLVGRYEIVNFDD
                     LFTEVEFGDGVITAFETDDRTQQLITLGLSYRPIPLIGLIFNVEIGDLIEGDALIFPQ
                     DVEADKFFAFTAGGVFGF"
BASE COUNT          988 a          817 c          602 g         1062 t
ORIGIN      
        1 gggttgtctc taatagttcg aggtaggtct ttgggtaacc gctttcgaca cctctgtagt
       61 attctatcaa tccattatag ctaaagcctg tttttgcata agagattaat agatttgttg
      121 cgatacctat caacataaaa agaagaaaaa tagtcgttac gatttttatg ctctgtgaag
      181 cgctcgaaag cgtatacttc tgcataaagt tctgcatgtc gctcttcaat taatagtttt
      241 aattacttcg agtttactat aagaaggatt tttatatata caaagctttc cttttttttc
      301 tctaactatc ataacaccta catcctttaa acgctctatg aattttattg cattatccct
      361 cttcatcacg gaaagagctg ttgaaagtgc atccgcatcc gttgcatcat gagctatcac
      421 cgttacagac gggacagatt caactggcaa tccattgcgt gggtcaataa tatgtgagaa
      481 gcgccttccg tccacctcaa agtatctctc ataatctcca gatgtagata ctgctatatc
      541 ttcaacctta agtaaggtaa gcacttcggc ttcatccctt gggtgcctaa tcccgacatc
      601 ccatccatcc tcccctggag ggggctgcat agcatagatg ttcccgccga agttgacaat
      661 accccttttg ataccatagg atctaagaat ttttacggcc ctatccaggg cataaccctt
      721 acctatccct ccaaaatcga gcgaaaggca aggggattgt aactctattt gtttttccct
      781 tgggtataac tttattttgt caaaccctat acaatcttga gctctttgta actcatcacg
      841 attaggcatt aagtttttct cttcacttac cttccagatt tcgattgcct ttcctacagt
      901 tatgtcaaaa gctccgtcgg ttaaacctga gaaaaaaata gcccgacttg ttagcttgac
      961 gaactccggt ggaactgaaa tcctgccatc tcctgcaaat gagtttactc gtgagagcac
     1021 gctattgttt ttataattac tgaaaatatc gtctagcctc tttacttctg aataggcttt
     1081 taagatagct ttatcacaag cctctttatt ctcgcagtaa agcctaaact caagcatcgt
     1141 ccccattaca aaaaaggctc tttcaacaat aacctgtgga agggctagaa agattgcgat
     1201 aatgggtata agcttcatct tatctctcaa agggcaagtc agaagtcttg cttctaccgc
     1261 ccaccacaaa gactggtttc ctaccctgtt taaactgcct cctctttagt tgtgtaattt
     1321 ccatcttaac ctaaaaaagt ttttgcaaaa gccttcattt tcctattcca ccgcattttc
     1381 cccttgtaaa taaaccacac ctattagggc cagtgccttt ttaactccca catttgcagc
     1441 cctcacagaa agggttgcac ctgatatatt cacaatatcc ttcctagtcc ttatgggatc
     1501 atcaagcgtt tttccctcat attgtttgag gaaccttttt gcccttatct cgcttccata
     1561 tggctctctg tagaccataa cggctatatt ttcggcctta ccctcaggag taacacctac
     1621 gataaacgtt atgggtctat gtttgccaat ctcatcaaca ataaccgcat atccaatgac
     1681 tttgctatca ttctttgcaa taaacgtttt atattctttc tcccatatag atggttcaac
     1741 cttccctatc aaacccttta ccttttcacg aagctcagga gtagactcaa ctactcttat
     1801 ttcaaattcg tctgcctctg gaaagaccat cttcggtgct tcttcttcag aaagatatat
     1861 cttgtctgca ctgattgcca gacagcgtaa aacaagaacc aagaaaaagg tgaaggcgaa
     1921 cgcacttatt cttgatttca tctatcatcc atactgtagg ttgggattag tcgttgtgag
     1981 cctttaatca tatcatgatg aatggagaaa ccaaacacac ttgagaagag aataaacaat
     2041 aatacggact taattatcgt tttaattata ctgaacacta atgccattag tattttcctt
     2101 tcatattttt agagagagcc gcctctcctc atggcacaag tcatatagag ccaatacaag
     2161 gttctagcct agaagtatta tcagaattag atttaagtcc aggagtctca tttaagatct
     2221 taaacctatc taacctttaa aatccaaaca cgccaccagc agtaaaggca aagaatttat
     2281 ctgcctctac gtcctgtgga aatataagcg cgtcgccttc tataagatca cctatttcaa
     2341 cgttaaatat aagaccaata agtggaatgg ggcgatagct aagtccaagg gttataagct
     2401 gctgagttct atcatcagtt tcaaaagctg ttatcactcc atctccaaat tccacttctg
     2461 taaagaggtc atcaaaattt actatctcat aacgaccgac aagcctcagg tcaggatttt
     2521 caaatggcga tccgagaggg gttttattaa gaaactcagg ccagaagtga taggtaagag
     2581 ataaccaaaa accttgcttg ttttttgcaa tcatggggga ttcaaatgca agttctacaa
     2641 taaggtcatc aatctctccc tcaacctcgg agttgactgt aagctcagcg aacctttcta
     2701 ccacttgctg agcatcttca aaatgtgtat atatgtactc tgcttctata tcgaatggac
     2761 cgattgagaa tttagtgtca cccccgaatg cagttatgta cttgttaata cctaggaagt
     2821 caggggtata ctgaccaaca taaaaagacc ctccgacctc agcaccaagg aatggactat
     2881 acgcaatcct tccggtaaac gccttattac cgtttaagtc cgctgaaaaa cctcctgggg
     2941 acaaatcaag cgtcccttca agctcaatct tactctcatc tggggtagtt tctatcagag
     3001 tctcaatctg accctcgagt gtagccccgt tcattacgta aaactggtag ttaagtcctc
     3061 ctttatctcc taaatcaaag ttcccgttaa gcccagctcc aagttcatcc cacgccgcgg
     3121 caacaggtag cactggaccc tgtcggtctg ttatggtgcg gcgattgaaa tcatagaggt
     3181 tatcatcatg gtacagatta aatctaccta atgggacaag aatagcccca agtctgaggc
     3241 cgtgattctc agcataatca aattgtagcc atgcttgctc taaggcaatc tcgctttcat
     3301 ttgaagattc aatctcctgt tcaaaggtaa ggcctccttc ctgcgatgtt gtgctctctt
     3361 caacctctat ttgtaaaaat ctctcaaact caagctcagt atatatgcta attctatcta
     3421 caggtcttgc atcggctgta agaacaagac ggcggagtgt aaaggtatt
//
LOCUS       DATDRL010000380         5641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U7976
            k141_2021803, whole genome shotgun sequence.
ACCESSION   DATDRL010000380 DATDRL010000000
VERSION     DATDRL010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962740
            Sequence Read Archive: SRR3989307
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 11:37:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,480
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 6 of 24
            Pseudo Genes (multiple problems)  :: 6 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2021803"
                     /isolate="SMAG_U7976"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1004
                     /locus_tag="VI935_12555"
     CDS             225..1004
                     /locus_tag="VI935_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HLE26460.1"
                     /translation="MKLNGKVSIVTGSASGIGFAIAQALAKGGANIVIADIDEKGGKK
                     AVKTILKIGRNSLFVKTDVSNRVDCKKLIDKTVRKFGRIDILVNNAGLQHVAPITDFQ
                     EDKWDLLINVMLTGTFLCTKYALPYMIKQKSGRIINISSVNGLIGMKFKSAYVSAKHG
                     IIGFTKTLALEMAEHNITANAICPTFVRTPLVDKQIDDQARAHGILREEVIEKVILAE
                     APMKRLLEPEEIADLAVYLASDSARSITGSAITIDEGWTAQ"
     gene            1260..1598
                     /locus_tag="VI935_12560"
     CDS             1260..1598
                     /locus_tag="VI935_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLE26461.1"
                     /translation="MKKVLMILLAWVMLFGNLVFPTTTFAEPGSKVINKRLYRQKGRI
                     FHGINNGSLTRREARRLGAEQAKIRVDKFYAKHNDGHIGPRELIYLNHELNHSSRHIY
                     RLKHNDWSRW"
     gene            2069..3682
                     /locus_tag="VI935_12565"
     CDS             2069..3682
                     /locus_tag="VI935_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HLE26462.1"
                     /translation="MKFKYQNLCRSWKWEIPSYFNIGRDCTDKYAKSESHGNKVALYW
                     ENEDGETKKYTFTEVARLTNQIANALLSLGFQKGDRLLIRLPNLPEFPLVFLGAIKIG
                     AVPIPSSTMLTAEEIDYLLYNSKSKGVITTPELYEAVEVNRGHHKEFNHVLIDGQPIP
                     SQCVDFTELINKCSKNLSVAETKADDLAYICYTSGTTGFTKGVVHAQRALIGHDPAAR
                     YWQALKPDDIVMHAGKLNWTYTLGTGCLDPWRLGCSTVIYGGEHNPKKFFELITKYGV
                     NVFMAVPTVYRQMLRVSDEIDCDLSSLRHALSAGEHLSEELFHSWKNELGVELYDGLG
                     MSELSYYVSNMPGMPIKPGSPGKPQPGHRSTLIDEEGKEVDVGEIGVLATPKDDPGIM
                     LRYWEKPEETDNMFCGDWFVSGDFFYKDEEGYFWLVGREDDIITTFGYRVSPFEVERV
                     LSEHPIVKECAVTGIDVGEDKTITTAFIVLKDSRHELEKLKEELLDYSHRRLAKYKCP
                     REIIFLESLPKTPNGKIKRKVLRESHSIL"
     gene            complement(3701..4642)
                     /locus_tag="VI935_12570"
     CDS             complement(3701..4642)
                     /locus_tag="VI935_12570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLE26463.1"
                     /translation="MRRIATVSPVWGCSVEDLRAIAREAEEAGFEAIFSPEVPPYSAI
                     ANAQVFAEATSKIKVGTWITSIYMRQAVMCASEALTVQEVSGGRMVLGLGVSHKPVVT
                     DRFGIDMGDPIEEMRKYVKAIRSFADGSSSLLTIKRQLPAFPIYTAVLTEKAAELAGE
                     VSDGIMPYMATPEYIKKLASAVRRGAQKAGRSPSDIDITDGIPSFISDDLDAARKAAK
                     RGLSGYARFPYYQRLIKNIGFGDVIEKIKSGANPADVITDELVDSVALIGPAGRCCER
                     LEALREAGVRLPIIVPNPVGKQSNIEVMQIMIKALVS"
     gene            complement(4762..>5641)
                     /locus_tag="VI935_12575"
     CDS             complement(4762..>5641)
                     /locus_tag="VI935_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLE26464.1"
                     /translation="MPLFRPPSEAKSFILQATIGCSHNECTYCAMYREETQKFRIRPT
                     DEIKAIIDEASETDDFDRVFIADGNALSIPQKRLVEIMEYLNKKCPHLERISMYANIG
                     DILRKGVENLKALRELGLQMVYIGFESGDEVVLERIKKGANYEQTVRACKMLKEAGIK
                     NSAMVLLGVGGVDRSLEHAEATGKLLTDTDPEYVGALSLQIRPGAPIYQESLEGTFQL
                     PDKFQMIKELEIIVENTNLTDGYFFANHISNYLPVKAKFPEDKEMVLEEIRRVLRNRD
                     ESVLRPEYYRDVINQY"
BASE COUNT         1728 a         1173 c         1139 g         1601 t
ORIGIN      
        1 agcgagagcc atgcccatga taagtctcat ttaagcttac ctcccgtatc cgatttatta
       61 cttagtgagg atagttactg tatgatattg ccatgctcca acgcctattt caatcactat
      121 gcactaaata tcatttttgc aagcttttat tcttcttgta agatatacca aaataagttg
      181 tatgagattt tgttatctaa aagtaataaa ttaaggagtt aacaatgaag cttaacggca
      241 aggtcagcat agtaacaggc tcagcaagcg gcataggctt tgcaattgca caggcactcg
      301 cgaagggggg tgctaatata gtcatagcag acatagatga aaaaggtggg aagaaagctg
      361 ttaagacgat tttaaaaata ggcaggaatt cactttttgt aaaaactgat gtgtcaaacc
      421 gtgttgattg taagaaacta atagataaaa ccgtgagaaa attcggtcgg attgatatcc
      481 tagtaaataa tgcaggcctt cagcacgtag ctccaataac cgatttccag gaagataaat
      541 gggatttact tatcaatgtg atgctcaccg gaacctttct ttgtacgaaa tatgccttgc
      601 cttatatgat caaacaaaaa agtggaagaa ttataaacat ctcatcagtt aacggactta
      661 tcggtatgaa attcaaatcc gcctacgttt ctgcaaaaca tggcataata ggttttacaa
      721 agaccctggc ccttgaaatg gctgagcaca atataacagc taatgctatc tgtccaacct
      781 tcgtcagaac tcctctcgtt gataagcaaa tagatgatca agcaagagca cacgggattc
      841 taagagaaga ggtcattgaa aaagtcatcc tagctgaagc gcccatgaaa aggttgctcg
      901 aacctgagga gattgcagac cttgctgtct atctagcgag tgattcagca cgtagtataa
      961 cagggtcagc gataactatt gatgaaggat ggacagcgca gtaataaatt agtgaagaga
     1021 aataaataca gatattactt tttatctcaa aaaactcctt ttaatttcaa gcattcaata
     1081 aacctaattg taaaaatcgc cttatacaaa actccatcca acttataaat catcttacga
     1141 aattgcttaa acaaataacc tcgaaacagt acgcttccta atagtaattt gttttatgaa
     1201 accttttgct aagaagataa gttaatattc aacaaacaaa gcagaaggag ggaataaaga
     1261 tgaagaaggt tttaatgatc ctgttagcct gggtaatgct gtttggtaat ttggtttttc
     1321 ctacgacaac gtttgcagag cctggttcaa aggtaataaa caaacgacta tatcgtcaga
     1381 aagggcgaat ttttcatggg atcaataatg gttcactaac acgacgtgag gcaagacgcc
     1441 ttggagctga acaagcaaag attagagtag ataaattcta tgcaaagcat aacgatggtc
     1501 acataggtcc acgcgagtta atctatctaa atcacgagct taaccattcg agccggcata
     1561 tttaccgcct gaagcacaac gattggagtc gctggtagca attagcttaa ataggatagt
     1621 gaagaatata tcttatagct tcactctcca ccatcttgtt tccttttttg caccttgcaa
     1681 atgtttctct caaatgggat ctcgatcacg agccagactt ttcgcctaca ccgagaatgt
     1741 gatgttacga aatcagagcg ggacaagaat gtcccgccta tcaggttcgg tgggcgaata
     1801 ccgcgatagg cggggttttc taaccccgct acaaacatag gattacttca cggagtctac
     1861 attgagcaca ttcgcttcaa tcagtgcaca ctgcgcgaat gtgttcgcaa tgacatctga
     1921 aaccctgtag aaagctaaag gaaattaatt cgttaaaatt agtcatatta attaccaact
     1981 ggacggctgg gcaaggcctt taaaaatttg aaattttatt taatcaatgt aattttctaa
     2041 cgattaaagc acctcaaaca tatatcagat gaaatttaaa tatcaaaacc tatgccgttc
     2101 ttggaagtgg gaaattccta gttactttaa catcgggcgt gattgcacag ataaatacgc
     2161 aaaaagtgaa tcccatggaa acaaggtcgc attatattgg gagaatgaag acggtgagac
     2221 taaaaagtat acattcacgg aagtcgcaag gcttacaaat caaattgcaa acgccttact
     2281 ctctcttgga tttcaaaaag gggaccgtct tcttatacga ctacctaacc ttccagaatt
     2341 tccccttgtt ttcttaggag cgattaaaat tggtgctgtt ccaattccaa gtagtaccat
     2401 gcttacagcc gaagagattg actatctgct ctataacagt aaatcaaaag gagttatcac
     2461 tacaccagaa ttatatgagg ctgtagaggt taatcgagga catcataaag aattcaatca
     2521 cgtgttaata gatggacaac ccataccttc tcaatgtgta gatttcaccg aattaataaa
     2581 taaatgttct aaaaatctaa gtgttgctga aaccaaagct gatgacttag cttatatctg
     2641 ctatacatca ggtacaacag gttttacaaa gggcgtggtt catgctcaac gcgcattaat
     2701 tggacatgat ccggctgctc gatactggca ggcactaaaa ccagatgata ttgttatgca
     2761 cgcagggaaa ttaaactgga cctacacctt gggcacaggt tgccttgacc catggcgtct
     2821 gggatgttca acagtaatat acggagggga acataacccg aaaaaatttt ttgagttaat
     2881 tactaaatat ggagtaaacg tatttatggc tgtccctacg gtttatagac agatgcttcg
     2941 ggtatcagac gaaattgatt gtgacctttc tagtcttcgg catgctctta gtgcaggtga
     3001 gcatctaagt gaagagttat ttcattcttg gaaaaacgaa ctaggcgttg agctttatga
     3061 cggacttgga atgagtgagc tcagttatta cgtatcaaat atgccaggta tgccaataaa
     3121 acctgggtct ccgggaaagc cacaaccggg acaccgctcc accctgattg acgaggaagg
     3181 aaaagaagtc gatgtgggag aaatcggtgt actggctaca cctaaagacg acccgggaat
     3241 tatgctccgc tactgggaga aacctgaaga aactgacaac atgttttgtg gagattggtt
     3301 tgtaagcggt gatttcttct ataaagatga agagggatac ttctggcttg taggaaggga
     3361 agacgacata ataactacat ttggttatcg tgtctctcct tttgaggtag aaagggtttt
     3421 aagcgagcat ccaatagtaa aagaatgtgc tgtgaccggt attgatgtcg gagaagataa
     3481 aacaattaca acagcgttta ttgtacttaa agattcaagg cacgaattag aaaaattaaa
     3541 agaggagctt ttagattact ctcatcgccg gctggccaaa tataagtgcc ctagagaaat
     3601 aatcttcctt gaatcacttc ctaaaactcc aaatggaaag attaaacgaa aggtgttaag
     3661 agaaagtcat agtattctgt aacacttttc gaataatcga ttaactcacc aatgccttaa
     3721 tcatgatttg cattacttct atattactct gcttaccgac tggattaggc actataattg
     3781 gtagccgaac ccctgcctct cttaaggcct cgagcctttc gcaacatcgt ccagcaggac
     3841 caattagagc gactgaatca actaattcgt cagtaatcac atcggcagga ttcgcaccag
     3901 actttatctt ttctatgaca tccccaaatc ctatgttctt aataagacgc tggtaatagg
     3961 ggaatctcgc atacccagaa agtccccgtt ttgccgcctt tcgggctgca tccagatcat
     4021 ccgaaatgaa gctgggtatt ccgtcagtga tgtctatatc tgatggagag cgaccggctt
     4081 tctgagcccc tcttcgaaca gcgctagcaa gtttcttaat atactcagga gttgccatgt
     4141 agggcatgat tccatctgaa acctctcccg ctagctccgc cgccttttcc gtcagtacgg
     4201 cagtgtatat tgggaatgcc ggtagttgtc tcttaatggt cagtagtgaa gagcttccat
     4261 ccgcaaatga tcttatcgct tttacgtatt ttctcatctc ctcaatcggg tctcccatat
     4321 caattccaaa acggtctgtt acaactggct tatggctgac cccaagcccc agcaccatcc
     4381 tccctccgga tacctcctga accgtaaggg cttcagaagc acacataact gcttgacgca
     4441 tataaatact agtaatccaa gtaccgactt ttattttgga tgttgcctcg gcaaagactt
     4501 gtgcattagc gatagcacta tagggtggaa cctctggtga aaatattgct tcaaatcccg
     4561 cttcttcagc ttcacgggca atggcacgga ggtcttcaac actacatccc catacaggcg
     4621 aaacagttgc aattcgtctc atttatcttc tcctttttta attgtagcca cgaattttca
     4681 cgaatgagta cgaatataac cgaataatta tgcacgatgc atgatagaga taatacattt
     4741 catcttgcat cctgcatcct gttaatactg attaattaca tccctgtaat actcaggacg
     4801 aagaactgat tcatcacggt ttcttaatac ccttcgaatt tcttctaaaa ccatttcttt
     4861 atcctcaggg aattttgcct taactggaag atagttagaa atgtgattag caaagaaata
     4921 tccatcagta agatttgtat tttcaactat aatctcaagc tcttttatca tctgaaactt
     4981 gtcagggagt tgaaaggttc cttcaagcga ttcttggtag attggagcac caggacgaat
     5041 ctgaagcgag agtgctccta cgtattcagg atccgtgtca gtaagcaatt ttccagttgc
     5101 ttcggcatgc tcaaggcttc tatctactcc gccgacgcca aggagtacca ttgccgagtt
     5161 cttaattcca gcttctttaa gcatcttgca ggcccttaca gtttgctcat aattggcgcc
     5221 ttttttgatt ctctcaagaa caacttcatc gccgctttca aatccgatat agaccatctg
     5281 aaggccaagt tccctaagag ctttcaaatt ctcaacccct tttcgcagaa tgtctccgat
     5341 gttggcatac atgctaatcc gctccaggtg cggacacttt ttatttagat attccataat
     5401 ctccacgagt ctcttttgag gaattgaaag ggcatttcca tcggcgataa agacccggtc
     5461 aaaatcatct gtctctgaag cctcatcaat aatcgcttta atctcatctg tcggacgaat
     5521 gcggaatttt tgagtttctt cgcggtacat cgcgcagtat gtgcactcat tgtgtgagca
     5581 gccaatagtt gcttgaagta tgaagctttt tgcttcactg ggcggtctaa aaaggggcat
     5641 t
//
